LOCUS       DAOSYE010000001         9072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_4887_length_9072_cov_3.028502, whole genome shotgun sequence.
ACCESSION   DAOSYE010000001 DAOSYE010000000
VERSION     DAOSYE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_9072_cov_3.028502"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLU43_00005"
     CDS             <1..378
                     /locus_tag="PLU43_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO26816.1"
                     /translation="TLQIFYYSSCMFIFVLGLCYVTCSIIIFFRDLGQIISIALQVGI
                     WATPILWNLNIMAESHPKLKVIFKLNPMNYIVEGYRNALIDKIWFWETFYSTAYFWIL
                     TLCVFALGALIFKRLKIHFADVL"
     gene            395..1669
                     /locus_tag="PLU43_00010"
     CDS             395..1669
                     /locus_tag="PLU43_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO26817.1"
                     /translation="MEVSTAIQIDHLTKVYKLYDRNRDRLKEALHVGKNIHCREHYAL
                     NDVSLRVHTGETVGIIGTNGSGKSTILKIITGVLNPTAGDIQINGRISALLELGAGFN
                     MEFTGIENIYLNGTMIGFTEEEINEKLQDILDFADIGEFVNQKVKTYSSGMFVRLAFA
                     VAINIDPEILIVDEALSVGDVFFQNKCYRKFEDFKKQGKTILFVSHDLSSISKYCDRV
                     VLLEKGRKIAEGEPKEIIDVYKKVLVGQLDKKADTCKSAKLAGSRWKEQMNLNPNFDE
                     YGSGLAEFEDYCAYDNTGTITNTIIKGEEFTVKLKIRFFESIQDPIFAVSFKNMQGTE
                     ITGTNTMFEKITTGTPKPGDVMTATFTQEMSLQGGEYLISLGCVGYREGNFTVYHRLY
                     DVFNLTVISSKNTTGYYDMNSIVTVRKEDEDE"
     gene            1662..3995
                     /locus_tag="PLU43_00015"
     CDS             1662..3995
                     /locus_tag="PLU43_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO26818.1"
                     /translation="MNKEKMDAIGNVVINYSYYSGEDVYSEGISEDILLDLVQRYRES
                     EFEHVIQNSRSWSVMYHLSYIRENIVSWLPIEKTAKVLEIGSGCGAITGKLAEMAEKV
                     TCIELSKKRSLINATRHKEYDNIEIIVGNFMDIEKEITETYDYITLIGVLEYAESYIG
                     GTDPYRGLLRLIGKHLAPGGKIVVAIENRFGLKYFAGCKEDHTGGYFDGIEGYPNSQG
                     VKTFSKEKLALIMEEAGFSAKFYYPYPDYKLPHTIYSDEYLPKAGELTTNLRNFDADR
                     VVSFDESKVFDSLIEEGLFSHYSNSFLVLASAEDIWDISPVIPVFAKYANDRSVQFRV
                     ATMIMQDKTGNKFVYKAALNTKTNPHISAIYSNYQTLTGIYEGTKLKPNICKFIQGVE
                     AAPPIAGVSSKAKHKVELTYLRGITLEKYLDMLEAYSDYDKMLALIREYQALIAQVGG
                     QGVFTMTEEFKEIFGECRFEKEYAASFICNYDMIFSNIVLDETTKENGEWNVLDYEWL
                     FKFPIPFQFIVYRSLYYYFENRGSSGFLTYLDEKGTDIYKECGIDEKEQELFAEMEHR
                     FQVYIISGVASLEVMQVMMPSTTINLGRLLVLGSYLRNLNTPKIYFSCGEGFFTENQI
                     HVLANVTEGRLVTMEIPLAHNMVGLRIDPTEYPCVVHINKVELTCLDGSIEDITRYLI
                     NGYAISEDTLLFDTDDAQIILEKIPHGARKLCVEYQVTMFTWIFFNEIRDMLIRKREC
                     ERVKPTILDRILIKCRMKKWEELPEGFSYNKESEEKE"
     gene            3998..6031
                     /locus_tag="PLU43_00020"
     CDS             3998..6031
                     /locus_tag="PLU43_00020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO26819.1"
                     /translation="MGLFDRKKLYTAFFNELKKQTQPYAYFLMEEENEARGVQYKSSP
                     LVCMIGSFFTEYIVENYEWMDEEYLVFIYKNGEPAKGAVAQLFSKTDKLKPDLIYCDE
                     DFITVPERRRHTPWMKPDWSPDTLLSLNYIGSLFAVKKSEAEQIRLLTGEECAGDDVR
                     MYDFLLKYTEKINKITHVSKILFHRFGKEEQITQMITDEYLSEKYKAVRENAFLRRGF
                     QKERRDRVSIIIPSKDHARILLQCLESIKNKSKFNNLDTEASAEKTLEIIIVDNGSCE
                     EEKNKITDYIKKNPAMQIFYLYEPAPFNYSAMCNQGAREANGEYLLFMNDDVEVKDSD
                     FIEKLLYYAAAPHTGAAGAKLYYPGSDLIQHTGVTNLDCGPSHKLAMHTDAAVYYFGR
                     NRFNMDVLALTGACMMMSKEKYFKVGGFSDKMRVSYNDIDLCVSLYEQGFYNVIVNDC
                     VLYHHESLSRGADQADQAKYERLAKERELFYTKHPWLRNRCDPFYNRNLIQDTLDYRV
                     NVQADYEKRDFRNPAAVMKRHLFIRTERKLKFNIERTGREQAFDKKAEDAYGFEGWAL
                     LQKGDNALYERYLCLIPKMQVSKRAAFINIKTKNSLQASVSPKYRTDVKDVFGNAENA
                     ELAGFCCRIPVSLIEEEQIYKVGMLFVSKISNRKYMTLGDYYATGRGYFTEEV"
     gene            5994..8045
                     /locus_tag="PLU43_00025"
     CDS             5994..8045
                     /locus_tag="PLU43_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO26820.1"
                     /translation="MPQEEDILLRKYEYYKALYEKELAKNAELTEALVDVEAQNADLT
                     YKLDKIRKSRLWKSIYPLRLLWSHTKNFFIRVRRYGNLKNLMRKIESKRIEKKACKSY
                     GTLSMPSPETVKAQRNTEFSKNIKFSILVPLYNTPEKFLREMLASVENQTYQNWELCL
                     ADGSDGEHADVGRICAEFAAQDEKAAGSAGFSESRVKYVKLAENLGISGNTNACFALA
                     TGEYIGLFDHDDLLHPCALYEYMKVICEQNADYIYCDETTFCGSSIDHMITLHFKPDF
                     AIDNLRANNYICHFSVFSKELLAQTGFFRSEFDGSQDHDMILRLTMAAKKIVHVPMIL
                     YYWRSHAGSVAADINAKTYAIDAAKGAVSAHLTACGFEKFKIESSRAFETIFRIRYHL
                     TEKPLISILIPNKDHVGDLRRCMDSIIERTSYENHEIIIIENNSTDPDTFSYYEALEK
                     HPSVRVVTFKGSFNYSSVNNYGAQFAAGRYLVFLNNDTKVVTRSWLEELLMYAQRQDI
                     GAVGCMLYYEDYSIQHAGIVLGLGAHRTAGHSHYKMSKDNLGYMGRLCYAQNVSAVTG
                     ACMMTKKVLFDELGGFSEEFAVALNDVDYCLRLRERGYLNVFTPFAELFHYESKSRGT
                     DVSDQATKENAARYDRESELFRNKWKTVLEAGDPYFNPNFSLDYSNFVLKGNPKSMVE
                     N"
     gene            8067..>9072
                     /locus_tag="PLU43_00030"
     CDS             8067..>9072
                     /locus_tag="PLU43_00030"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOO26821.1"
                     /translation="MDYMSQYKFWREDDYFDTATKEELAGLEGNDAEIQDRFYKELEF
                     GTGGLRGVIGAGTNRMNLYTVRKATQGLANYIIKQGAQAKGVAIAYDSRFMSPEFADE
                     AALCLCANGIKAYVFSSLRPTPELSFAVRYLGCTAGIVVTASHNPPEYNGYKVYWEDG
                     AQITAPKDTDIITEVKNVTDYHDVKTIGKEYAIADRLYSVIGETIDDAYMEQLKKLII
                     HPEIIKEAANDIRIVYTPFHGTGNLPVRRILSELGFKHVFVVPEQELPDPNFTTLEYP
                     NPEDPKAFTLALKLAKEKDADIVLATDPDADRLGIYAKDAKTGEYVSFTGNMSGMLIA
                     E"
BASE COUNT         2761 a         1559 c         2296 g         2456 t
ORIGIN      
        1 acccttcaga tattttatta ttcttcctgt atgtttattt ttgtgcttgg tctttgctat
       61 gtgacctgtt cgatcattat ctttttcagg gatctcgggc agatcatatc aattgcgctg
      121 caggtcggga tctgggcaac accgatttta tggaatctga atattatggc cgaaagtcat
      181 ccgaagctga aagtcatttt caagctgaat ccaatgaact atatcgtaga gggatacagg
      241 aatgcgctga tcgataaaat atggttctgg gaaacttttt attcaacggc ttatttctgg
      301 attttgacac tctgtgtttt tgcactgggt gcgctgatct ttaagagact gaaaattcat
      361 tttgcggatg tgttataaac agacaggaga ctatatggaa gtatcaacag caattcagat
      421 cgaccattta acaaaggtat ataagctgta tgaccgcaac agggaccggc tgaaggaagc
      481 gctgcatgtc gggaaaaata ttcactgcag ggaacattat gcgctgaatg atgtcagtct
      541 tcgggtacat acgggagaga cagtcggtat tatcggaacg aatggttccg gtaaatccac
      601 gattttaaaa atcattaccg gtgttttgaa tccgacggcc ggtgatattc agatcaatgg
      661 ccgtatttcc gctcttttgg aattgggagc cggttttaat atggaattta caggaattga
      721 gaatatttat ctgaatggta cgatgatcgg tttcacggaa gaagagatca atgagaaact
      781 gcaggatatt ttggattttg cagatatcgg tgagtttgtc aatcaaaaag taaagaccta
      841 ttcaagcggt atgtttgtac gtctggcatt tgccgtagca atcaatattg atccggaaat
      901 actgatcgtt gatgaagcac tttccgtggg cgacgtattt tttcagaata agtgctatcg
      961 gaaatttgag gactttaaaa aacagggaaa aacgatattg tttgtcagtc atgatctaag
     1021 cagtatcagt aaatactgcg acagggtggt cctgcttgaa aagggcagga aaattgctga
     1081 gggtgaaccc aaggaaatca ttgatgtcta caaaaaggtg cttgtcggac agcttgataa
     1141 aaaagcagat acatgtaaga gcgcgaaact ggcgggaagc agatggaagg aacagatgaa
     1201 cctgaatccg aattttgacg aatacggaag cgggcttgcg gaattcgaag attattgtgc
     1261 gtatgacaat acaggcacga tcacgaatac gatcatcaaa ggcgaagagt ttaccgtgaa
     1321 gctgaagatc cggttttttg agagtattca ggatccgatc tttgccgtct cttttaaaaa
     1381 tatgcagggg actgaaatta cgggaacaaa tacaatgttt gagaagatca cgacggggac
     1441 gccgaaaccg ggtgatgtca tgactgcgac ctttacgcag gaaatgagtc tgcagggagg
     1501 cgagtacttg atctcgcttg gctgcgttgg ttatagagag ggcaatttta cggtctatca
     1561 cagattatat gacgtattta acctgacggt aatttcttct aaaaatacga caggctatta
     1621 tgatatgaat tcaattgtta cggtgagaaa agaagatgaa gatgaataaa gaaaaaatgg
     1681 atgcaatcgg aaatgttgta atcaattaca gctattattc aggagaggac gtttattcgg
     1741 aagggatcag cgaagatatt ctgctcgatc ttgtacagcg ttaccgcgaa tcggaatttg
     1801 aacatgttat tcaaaacagc cggtcgtggt cggtgatgta tcatctttcc tatatcagag
     1861 agaatatcgt aagctggctg ccgatcgaaa agacagcgaa agtgcttgag ataggttccg
     1921 gctgcggagc gatcacagga aagcttgccg aaatggcgga aaaagtgacc tgtattgagc
     1981 ttagtaaaaa gcgcagtctg atcaatgcga ccaggcataa agaatatgat aatattgaga
     2041 tcattgtcgg taattttatg gacatcgaaa aggagatcac ggaaacatat gattatatta
     2101 cactgatcgg tgttttggaa tatgcggaaa gctatatcgg gggaactgac ccgtatcggg
     2161 gcttgctgcg gcttatcgga aaacatctgg ctccaggggg aaagatcgtt gtagctatcg
     2221 aaaacaggtt tggactgaaa tactttgcag gctgcaagga ggatcatacc ggaggatatt
     2281 ttgacggtat cgaaggctat cccaattcgc agggtgttaa gactttttca aaggaaaagc
     2341 ttgccttgat catggaggaa gccggttttt ctgcgaaatt ttactatccg tatccggact
     2401 ataagcttcc gcatacgatc tattcagatg agtatctgcc taaggccgga gagctgacta
     2461 caaatttaag aaattttgat gcggacagag ttgtgtcttt tgatgagagt aaagtatttg
     2521 attctctgat agaggaaggg ttgttttcgc attattcaaa ttcttttctt gtactggcgt
     2581 cggcagaaga tatatgggat atttctcccg tgatccctgt gtttgcaaag tatgccaatg
     2641 accgatccgt acagtttcgg gtcgccacga tgatcatgca ggataagacc ggtaataaat
     2701 ttgtatataa agctgcttta aatacgaaaa cgaatccgca tatcagtgcg atctattcaa
     2761 actaccagac gctgaccgga atttatgagg ggacaaagct gaaacccaat atctgcaagt
     2821 ttatacaggg ggtggaggcc gcaccgccga tcgcgggagt gagttcaaaa gcaaagcata
     2881 aggtggagct tacctatcta agggggatta cacttgaaaa atatctggac atgctggaag
     2941 catattccga ttatgacaaa atgcttgctc tgatccgcga gtatcaggcg ctgatcgcac
     3001 aggtgggcgg acagggtgtc ttcacgatga cagaagaatt taaggagatt ttcggagaat
     3061 gcaggtttga aaaagaatat gccgcttcgt tcatctgcaa ttatgacatg atcttttcta
     3121 atatcgtact tgatgagacc acaaaagaaa acggagaatg gaatgtttta gactatgaat
     3181 ggctgttcaa atttccgatt ccgtttcaat ttattgtgta tcgttctttg tattattatt
     3241 ttgaaaacag aggatcaagc ggatttttga catatcttga tgaaaaagga actgatattt
     3301 acaaggaatg tggaattgat gagaaggagc aggagctgtt tgctgaaatg gaacatcgct
     3361 ttcaggtgta tatcatcagc ggggtggcat cgcttgaagt tatgcaggtc atgatgccgt
     3421 ctacgacgat caatctggga cgcctgcttg tacttggttc ctatttgaga aatttaaata
     3481 cgccaaagat ttatttcagc tgcggagaag gattttttac tgaaaatcaa attcatgtgc
     3541 tggcgaatgt tacagaaggc aggcttgtga caatggaaat tccgcttgct cataatatgg
     3601 tagggcttag gatcgatcca acagagtatc catgtgttgt tcatataaat aaagtagaac
     3661 tgacgtgttt ggatggaagc atagaagata tcacccgtta tttaataaac ggatatgcaa
     3721 tttcagaaga tacgctgtta ttcgatacgg atgatgcaca gattattttg gagaaaattc
     3781 cacatggtgc gaggaagctt tgcgtggaat accaggtaac gatgtttaca tggatatttt
     3841 tcaatgagat tcgggatatg cttatcagga aaagagaatg tgaacgggta aaacctacaa
     3901 tccttgacag aattctgatt aagtgcagga tgaaaaaatg ggaagagctg ccggaaggat
     3961 tttcttataa taaagaaagt gaagagaagg aatagaaatg ggcctgtttg atagaaagaa
     4021 gctttatacg gcattcttta acgaactgaa aaaacagaca cagccgtatg cgtatttttt
     4081 aatggaagag gagaacgaag caagaggggt acagtataaa agtagccctc ttgtttgcat
     4141 gataggaagt ttttttacgg aatacatagt tgaaaattat gaatggatgg atgaggaata
     4201 tcttgtcttt atctataaga atggagaacc ggcaaagggt gctgttgcac agctgttttc
     4261 taaaacagat aagttaaagc ctgatttgat ttattgcgat gaagatttta taactgttcc
     4321 ggagcgccgc aggcatactc cgtggatgaa gccggactgg tctcctgata cgttattgtc
     4381 actgaattat atcggaagcc tttttgcggt aaagaaatcc gaagcagaac agatcagact
     4441 gttaacagga gaggagtgtg ccggtgatga tgtcagaatg tatgattttt tgctgaaata
     4501 tacggaaaag ataaataaaa taacgcatgt ttctaaaata ttatttcaca ggttcggaaa
     4561 agaagaacag attacgcaga tgatcacgga tgaatatctg agcgaaaaat ataaggctgt
     4621 cagggagaat gcgtttctta gaagaggatt tcaaaaagaa agaagagacc gggtatctat
     4681 tatcattcca tctaaggatc atgcaaggat tttgctgcaa tgtctggaaa gcattaaaaa
     4741 taaatcaaaa tttaataatt tggacactga agcttccgcg gagaaaacgc tggaaattat
     4801 tattgtagat aacggaagct gcgaagaaga aaaaaataaa ataacggatt atattaaaaa
     4861 gaatccggcg atgcagatct tctatctata tgaacctgcc ccttttaatt attctgctat
     4921 gtgcaaccaa ggagcacggg aagcgaatgg tgaatatctg ttgtttatga atgacgatgt
     4981 ggaagtaaaa gacagcgatt ttattgagaa acttctttat tatgcggctg cgccgcatac
     5041 aggagcagcc ggagccaagc tttattatcc tggatctgac ctgatccagc atacgggcgt
     5101 gacgaatctt gactgtggtc cgtcacataa gcttgcaatg catacggacg ctgcagttta
     5161 ttattttgga aggaaccgtt ttaacatgga tgtcctggca ctgacagggg catgcatgat
     5221 gatgtctaaa gaaaaatact ttaaagttgg cggttttagt gataaaatga gagttagcta
     5281 taatgatatt gatttatgtg tcagtctcta tgagcaggga ttttataatg tgatcgtgaa
     5341 tgactgcgtt ctgtatcatc atgaatccct ttccagggga gctgatcagg cagaccaggc
     5401 aaaatacgaa agacttgcaa aagagcggga gcttttttat acgaaacatc cgtggcttcg
     5461 gaaccggtgt gatccgtttt acaacaggaa tctgattcag gacacgcttg attacagagt
     5521 aaatgtacag gcggattatg aaaagagaga tttcagaaat cctgcggcag taatgaaaag
     5581 gcatttgttt atacggacgg aaagaaagct gaaattcaat atagagagga ccggccgtga
     5641 gcaggcattt gataaaaagg cagaggatgc gtacggtttt gaaggctggg cgcttttgca
     5701 aaagggagat aatgcgctgt atgaacgtta tttgtgtctg attcctaaaa tgcaggtatc
     5761 aaaaagagca gcttttataa atataaaaac aaaaaacagt ctgcaggctt ctgtcagtcc
     5821 caaataccga acagatgtga aggatgtgtt tggtaatgct gaaaatgccg aactggcggg
     5881 gttctgctgt cgaattccgg tttccctgat tgaggaagag cagatctaca aagtcggaat
     5941 gctgttcgtt tcaaaaattt cgaacaggaa atatatgacg ttaggggatt attatgccac
     6001 aggaagagga tattttactg aggaagtatg agtattataa agcgctttac gagaaagagc
     6061 ttgctaaaaa cgcagagctg acagaggcgc ttgttgatgt ggaagcacaa aacgcggatc
     6121 tgacatataa acttgacaag atcagaaaga gcaggctttg gaagtcgatc tatccgctgc
     6181 gtctgctgtg gagccataca aagaattttt ttatacgtgt caggagatac ggaaatttaa
     6241 aaaatctgat gcgtaagatc gaaagcaagc ggatcgaaaa aaaggcctgt aaaagctacg
     6301 ggacgctcag catgccgtcg ccggaaacag tgaaagcaca gagaaacact gaattttcaa
     6361 aaaatattaa gttcagtatt cttgtaccgc tttataatac accggaaaag tttcttcgtg
     6421 agatgctggc gtctgtggag aatcagacat atcagaactg ggaattatgt cttgccgacg
     6481 gctccgacgg ggagcatgcg gatgtcggaa ggatctgtgc ggaatttgcg gctcaggatg
     6541 aaaaagcggc cggaagcgcc ggtttttctg aaagccgggt caaatatgta aaacttgcag
     6601 agaatttagg aatatcgggg aatacaaatg cctgctttgc acttgcaaca ggtgaataca
     6661 tcgggttatt cgatcatgac gatctgctgc atccgtgtgc gctgtatgaa tatatgaaag
     6721 tgatctgcga acagaatgcg gattatattt attgtgatga aacgacgttt tgcggcagca
     6781 gtatcgatca tatgattacg ttgcatttca aaccggattt tgcgattgat aatttaagag
     6841 caaataatta tatctgtcat ttttccgttt tttctaaaga acttttggca cagaccggat
     6901 ttttccgcag cgaatttgac ggaagccagg atcatgatat gattctgcgg cttacgatgg
     6961 cggcaaaaaa gatcgtgcat gtgccgatga tcctgtatta ctggcgttcc catgcaggca
     7021 gtgtggccgc cgatatcaac gcaaaaacgt atgcgatcga tgcggccaag ggtgcggttt
     7081 cagcgcatct gaccgcctgt ggttttgaaa aattcaaaat cgaaagttcg agggcttttg
     7141 aaacgatttt tcgtatcaga taccatctga ccgagaaacc gcttatttcc attctgatac
     7201 cgaacaagga tcatgtcggt gatctaagaa gatgtatgga ttcaataata gaaaggacct
     7261 cctatgaaaa tcatgaaatt atcataattg agaataacag cactgatccg gatacgtttt
     7321 cctattatga agcgcttgaa aagcatccgt ctgtcagggt cgttacgttt aagggaagct
     7381 ttaattattc gtcggtcaac aattacggag cacaatttgc cgcgggcaga tatctggtgt
     7441 ttttgaataa tgatacgaag gttgtgacac gcagctggct tgaagagttg ctgatgtatg
     7501 cgcaacgcca ggatatcggt gcagtcggct gtatgctgta ttatgaggat tattcgattc
     7561 agcatgcagg gatcgtactc ggactcggcg cgcatcggac agccggacat tcccattata
     7621 aaatgtctaa ggataacctg ggatacatgg ggcggctctg ctatgcccag aatgtatcgg
     7681 cggtgacagg agcctgtatg atgacaaaaa aagttctgtt tgacgaactc ggcggatttt
     7741 ctgaggagtt tgcggttgcg ctgaatgatg tggattattg cctgcggctt cgtgaaagag
     7801 ggtatttgaa tgtatttact ccattcgccg aactgttcca ctatgaatca aaatcaaggg
     7861 gcacggacgt atccgatcag gccacaaaag aaaatgccgc ccgctatgac agagaaagtg
     7921 aattatttcg aaataaatgg aagacagtgc ttgaagccgg ggatccctat tttaatccta
     7981 atttctcatt ggattacagt aattttgtac ttaagggaaa tccgaagagc atggtcgaaa
     8041 attaaatatt tgaaggagga ctgactatgg attatatgtc acagtacaaa ttttggagag
     8101 aagacgatta ttttgatact gcaacaaagg aggagcttgc cggactggaa ggaaacgatg
     8161 cggaaataca ggatcgtttt tataaggagc tggagttcgg aacaggcggt ctgcggggcg
     8221 tgatcggtgc cggaacgaac cgcatgaatc tttacacggt cagaaaggca acccagggac
     8281 ttgccaatta tattatcaag cagggcgcgc aggccaaggg tgttgcgatt gcctatgatt
     8341 cccgctttat gtcaccggag tttgcggacg aagcggcatt atgtctttgt gcgaacggga
     8401 tcaaggccta tgttttttcc tcactgcgtc cgactccgga gctttctttt gccgtgaggt
     8461 atcttggatg tacagccggc attgttgtga ctgcaagcca taatccgccg gaatataacg
     8521 gatataaagt gtattgggag gacggagcac agatcacggc accgaaggat accgatatca
     8581 taaccgaggt aaagaacgta acggattatc atgatgtgaa aacgattgga aaagagtatg
     8641 cgattgccga cagactgtac agtgtgatcg gggaaacgat cgatgatgct tatatggagc
     8701 agctgaaaaa gctgatcatt catccggaaa ttatcaaaga agctgcaaat gatattagga
     8761 tcgtttatac accgtttcat ggaaccggta atcttcctgt cagaaggatc ctttcggagc
     8821 ttggctttaa gcatgtattt gtcgttccgg aacaggagct gcctgatccg aactttacga
     8881 cactggaata cccgaatccg gaagacccaa aggcatttac gctcgcactg aagcttgcga
     8941 aggaaaagga tgccgacatc gtgctcgcga cagatccgga tgccgacaga ttgggaattt
     9001 atgcgaaaga tgcaaaaacg ggcgaatatg tatcctttac cggaaatatg tcagggatgc
     9061 tgattgcgga at
//
LOCUS       DAOSYE010000002         8353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_5482_length_8353_cov_3.602314, whole genome shotgun sequence.
ACCESSION   DAOSYE010000002 DAOSYE010000000
VERSION     DAOSYE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5482_length_8353_cov_3.602314"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2753
                     /locus_tag="PLU43_00035"
     CDS             <1..2753
                     /locus_tag="PLU43_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOO26822.1"
                     /translation="ESYRIILSAFTGDQNFRLIMDSSLKELDKKTEKYYYDAAVPYEV
                     ARLLPESDRAYLTIMEAVNESLNLLDLRKEYSKEYYSSLVQAQDYITKEFYDKYCGSS
                     MEQVYCVGHTHIDIAWLWTLAVTEDKAVRSFSTVLELMKQYPEYIFMSSQPQLYKYVK
                     KNAPAIYEEIKKRVAEGRWETEGGMFVEADCNLSSGESLVRQFLYGKRFFREEFGKDN
                     EILWLPDVFGYSAALPQIMKKSGIKYFMTTKISWNEFNKMPYDTFEWEGIDGTRILTH
                     FSPSRDYQKEAVEGGTETEHFTTYNAYINPSQVKGAWERYSQKYLNKEVLMSFGHGDG
                     GGGPTKDMLENQRRIAKGIPGCPRTVMSTAGDFFRKLETEVSGKKYLPLWVGELYLEY
                     HRGTYTSMARNKKYNRKSEFLFENAEAFGVLAERLKQTVYPKQEIEDAWEVILRNQFH
                     DILPGSSIKEVYEDSKKEYEEITRTGNRLIKHAFTQIVQNINAKESSVVVFNPNSFTG
                     EGIVSFSCPENIKFPVIADKDTIFPAQKTEDGSFIFCAAAVPSKGYQTFSIGEGMIKT
                     PLKADNEHLENELVRVTFHENGQISSIYDKCRMREVLQENRNANVLMTYEDRPHNFDA
                     WDVNNYYVEKAWEITELDSIKLVEEGPVRATICVKRSYLDSTIAQYISLLYNSPEIII
                     RNEIDWRENHIFMKSIFPVDIHTDEAVYEIQYGNVKRKTHYNTLWDYAKFEVCMHKWL
                     DVSEDGYGVSMLNDCKYGCHVHNGEIGISMLKSATYPNPQADKEHHSFIFSIYPHSGD
                     WKAAGTIKRAYAMNNPMCAVIKENKNGQLPEAYSLVSVDAENAVIEVVKKAEEGDALI
                     VRLYETYNKRTAVNMTFSEDIKKITECNMLEEEEAELPFDKKTASFILKPYEIKTCRI
                     II"
     gene            2772..4055
                     /locus_tag="PLU43_00040"
     CDS             2772..4055
                     /locus_tag="PLU43_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HOO26823.1"
                     /translation="MKKAATVEELIEVVKAEFADDKKIGKMFEKCFSNTLDTTVKKLP
                     DNTSFVITGDIPAMWLRDSVCQLRPYLILSGNNEEIAAVIAGLIKRQMEFIQKDPYAN
                     AFNEEANGMGHQEDHTEMKPFLWERKYEIDSLCFPLQFSYLYWKNTGKTNIFTEEWKQ
                     AAQTIVDTFRREQNHERNSAYFFERDHCPFTDTLSRSGKGALVRPNIGMTWSGFRPSD
                     DACVYGYLVPANMFASVVLCDLQEIAQTVYQDPALADSAGELGNEIKQSIADYAVLKH
                     AEYGEVYAYEVDGYGQYNLMDDANVPSLLSMPYLGFCRENDEIYQNTRRMILSETNPF
                     YFEGKALTGIGSPHIPGKYVWHISKAMEGLTCTDRTRKREIIEQLKNTDAGTFMMHEG
                     VYADDAAQYTRAWFSWANAMFSELVLSYCGYEVIR"
     gene            complement(4117..4779)
                     /locus_tag="PLU43_00045"
     CDS             complement(4117..4779)
                     /locus_tag="PLU43_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26824.1"
                     /translation="MKKQTNRKKTLAAAFITLIVLTISLSACSQETVNASVRQNQGTK
                     SGLSNGRGTGNKNTGNSSVTVSDELQASDSADSSSYGAAGALQEEDLTLREMLTYAIQ
                     DESLAHAEYEQIIAAYGNNNPYTNIIQAEETHISSLTTLFETYDIPVPEDLSSSYTIP
                     AATLLQAAQTGVTAEINNIEMYELFLTYDLPADVEKVFTSLMDASKNHLTAFEKQADR
                     LD"
     gene            4947..5642
                     /locus_tag="PLU43_00050"
     CDS             4947..5642
                     /locus_tag="PLU43_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO26825.1"
                     /translation="MKMLIADDNRQIVSILTEYGKKEGFETVPAYDGKQALELALTGN
                     FDMILLDVSMPVMDGFEVCRQIRRSLSVPVIMITARGEDFEKIMGLDIGADDYIIKPF
                     SPGEVMARVRAILRRIEKSTVSDGQVWNRQGLYLSLDDYTVRINGKEVGLTKKEMELL
                     WTLAVNSNKVFSRENLLNSVWGYDYFGDLRTVDTHIKRLRAKLDRFEHSGFELRTIWG
                     VGYKLEIMQGDGV"
     gene            5639..7123
                     /locus_tag="PLU43_00055"
     CDS             5639..7123
                     /locus_tag="PLU43_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO26826.1"
                     /translation="MKKISWKHQLTVRLVGYFFLVFLVFSVIIGFTFFYFFRANTIEL
                     HRAELEQRAEKIAETLGAMSADGIGRKQGNGYGAYLKYLGDIAMSDVWLIDKDRTIIT
                     DGDSVNSHVSYTDLPEAAENMIDEVFSGETLYSSDFDSVLSVPVITVGTPVISSTGEI
                     TGALLLHSSVDHVDLAVRNAFLIFLLSITAAFLIGMILAVRLSRKFTKPVCILSDITT
                     TMSKGEYGVRAGFDRKDEIGLLGNSIDILAQRLEELERQRQHLFSSVSHELRTPVTVV
                     QGSLEALIDGVVSGEDQKKEYFYQMLAETKNLSGLIDDILELSRLQNQEFVLTKGECF
                     INDIFSDLKRSLAVVAKRKGVGLVFEQECPAYGFYCDYNRIRQMFFVIYDNAIKYSDA
                     GKTVTTTFQLIFGRLQVTVSDQGIGIGKEELPHLFERFFRSERTGDIAGSGLGLSIAA
                     GIAKRHDIELSVTSEEGKGTAFSFLFPESGEKKSAAGGQKQHIV"
     gene            7149..8120
                     /locus_tag="PLU43_00060"
     CDS             7149..8120
                     /locus_tag="PLU43_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26827.1"
                     /translation="MFDLDQILGFYPLISSIVLLMLVITLCFVNMYLLARYRKSFFTI
                     MVHIGSFFVASGLIFYIVAHAIFAIPLSRFYARLAETALWTAVVICAAGFGYIAVNGK
                     KNDLRVLPFSITEVLQNIEDVVFVIDTDGIITHINHPALFQKMFGRIDTLEQLERHLQ
                     VSCFDTDRADGVLPDTGELYKCEGYFESLRMYAAFQIAPIMSLKGKSGYMIVIEDVTK
                     VRESEGALFKQNESLRNANERLGSYIKAAGQLEAEKERLVILKRIQETLLQDIEGALS
                     LIQGMKRSAFQDESYAGAMTGLASVLRSTYQKVRNAVSQIAGKGENV"
     gene            8117..>8353
                     /locus_tag="PLU43_00065"
     CDS             8117..>8353
                     /locus_tag="PLU43_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO26828.1"
                     /translation="MIRIVIADDHLLFRSMLEEILKRDPQLEVIGTCADGEEVLANYK
                     VWDPDLILLDIGMPKRGGIETLKTIKEKTPQIKVV"
BASE COUNT         2476 a         1488 c         2218 g         2171 t
ORIGIN      
        1 gggagtcata cagaattatt ttatcagcct tcacaggaga tcagaatttc aggctgatca
       61 tggattcctc gctgaaggaa ctggataaaa aaacagaaaa atattactat gatgcagctg
      121 ttccttatga ggttgcacgt ctgctgcctg agagtgaccg tgcatatctt acgataatgg
      181 aagctgtcaa tgaatcactg aatcttcttg atctgcgcaa ggaatattcc aaagaatatt
      241 acagcagtct tgtacaggca caggattata ttacaaaaga attttatgat aaatactgcg
      301 gcagcagtat ggaacaggtc tattgtgtcg ggcatacgca tattgatatt gcatggctct
      361 ggacacttgc cgttaccgag gataaagcag tcagaagctt ttcaacggta ttggaattga
      421 tgaaacagta tccggaatat atatttatgt caagtcagcc gcagttgtat aagtatgtga
      481 aaaagaacgc gccggcaata tatgaggaaa taaagaaaag agttgcggaa ggcaggtggg
      541 aaacagaagg cggtatgttt gtggaagcgg actgcaactt gtcgtccgga gaatcgcttg
      601 tacggcagtt cctgtatgga aagcgttttt ttagagaaga attcggaaaa gataatgaaa
      661 tattatggct tcctgatgtt ttcggatatt cggcggcgct gccgcagatc atgaaaaaaa
      721 gcggaatcaa atattttatg acgacaaaga tcagctggaa tgaattcaat aaaatgccgt
      781 atgatacgtt tgaatgggaa ggaattgacg gaacaaggat cctgacccat ttcagcccaa
      841 gccgtgacta tcagaaagaa gcggtggaag gcggaacgga aacggagcat tttacgacat
      901 ataatgcgta tatcaatccg tcccaggtaa aaggggcatg ggagagatac agtcagaaat
      961 atttgaataa agaagtcctg atgtcgtttg gccatggaga cggcggagga ggaccaacaa
     1021 aagacatgct tgagaaccag cgcagaattg caaagggaat tcccggatgt ccccgtacgg
     1081 ttatgagtac tgcgggagat tttttcagga agctggagac ggaagtcagc gggaaaaaat
     1141 atctgccttt atgggtaggc gaattgtatc ttgaatacca caggggtacg tatacgtcta
     1201 tggcaagaaa taaaaaatat aacagaaaat cagagtttct gtttgaaaat gcggaagctt
     1261 ttggagtgct tgcggaacgg ctgaagcaga ctgtatatcc gaagcaggaa atagaagacg
     1321 cctgggaagt catactccgc aaccagtttc atgatattct gcctggttct tctattaagg
     1381 aagtatatga ggattcaaag aaagaatacg aagaaattac aagaacagga aatcgactta
     1441 tcaaacatgc atttacacag atcgtacaaa atataaatgc aaaggaatct tccgttgttg
     1501 tattcaatcc gaacagtttt acaggagagg gaatcgtatc cttttcctgt cctgaaaaca
     1561 taaaatttcc ggtcattgcg gataaagata ctatttttcc tgcgcagaag acagaagacg
     1621 gttcttttat tttttgtgcg gctgcagttc cttccaaagg gtatcagacg ttcagtatag
     1681 gggaaggcat gataaaaacg ccgttaaaag cggataatga gcatctggaa aacgagctgg
     1741 tgcgggtaac ctttcatgaa aacggacaga tctcttctat ttatgataaa tgccggatgc
     1801 gtgaagtatt gcaggaaaac agaaatgcga atgtcctcat gacgtatgaa gaccggccgc
     1861 ataatttcga cgcatgggat gtcaataatt attatgttga aaaagcatgg gagatcacag
     1921 agcttgacag tataaaactg gttgaagaag gaccggtgcg ggcaacaatc tgtgtgaaga
     1981 gaagctatct ggattcaacg atcgcgcagt atatctcact gctgtataat agcccggaaa
     2041 taatcatacg caatgaaatc gactggcggg aaaatcatat tttcatgaaa tccatatttc
     2101 cggtcgatat tcatacggat gaggctgtct atgagatcca gtatggaaat gtaaagagaa
     2161 aaacgcatta taatacgctg tgggattacg cgaagtttga agtatgcatg cataagtggc
     2221 tcgatgtttc ggaagacgga tatggtgtca gtatgctgaa tgactgtaaa tacggatgtc
     2281 atgtgcacaa tggggaaatt ggaatttcca tgctgaaatc ggctacttat ccgaatccgc
     2341 aagccgataa ggagcatcac agttttatat tttccatata tccgcacagc ggtgactgga
     2401 aagctgccgg aacgattaag cgggcatatg cgatgaacaa tccgatgtgt gcagtgatca
     2461 aggaaaacaa gaacggacaa ctgccggagg catattcgct tgtcagcgtg gatgcggaaa
     2521 atgccgttat tgaggttgta aaaaaagcag aagagggtga tgcgctgatc gtccgtctct
     2581 atgagaccta taataaaagg actgctgtaa acatgacgtt cagcgaggat attaaaaaaa
     2641 taacagaatg caatatgctt gaggaagagg aggcagagct tccttttgac aaaaagacgg
     2701 cttcctttat tctaaagccc tatgaaatca aaacatgcag aataatcatt tgatacggaa
     2761 ggaatcaaat tatgaaaaaa gcagcaacag ttgaagaact gatagaagtt gtaaaggcag
     2821 aatttgcgga tgataaaaaa attggaaaaa tgtttgaaaa atgtttttcc aatacgcttg
     2881 atacaactgt aaaaaaactg ccggacaata ccagctttgt gatcaccggg gatattccgg
     2941 ccatgtggct gcgggattcc gtgtgtcagt taaggcccta tttgatattg tcagggaaca
     3001 atgaggagat cgcggctgtg atcgcaggac tgataaagcg gcagatggag tttatacaaa
     3061 aagatccgta tgccaatgct tttaatgagg aagcaaacgg aatggggcat caggaagatc
     3121 atacggagat gaagccgttt ctatgggaga ggaagtatga gatcgattcg ctgtgctttc
     3181 cgcttcagtt cagttatctt tactggaaaa atacagggaa gacaaatatt tttacagagg
     3241 aatggaagca ggctgcgcag acgatcgtcg acacgttcag aagagagcag aatcatgaac
     3301 gaaattccgc ctattttttt gaaagagatc actgtccgtt tacagacaca ctttccagaa
     3361 gcggaaaagg ggcgcttgta aggccgaata tcggaatgac atggtccggg ttccggccaa
     3421 gtgatgatgc ctgcgtgtat ggctatctgg ttcctgccaa tatgtttgca agcgtagtgc
     3481 tttgcgatct gcaggaaatt gcgcagacgg tctatcagga tccggcactg gcggacagcg
     3541 ccggagagct tggaaatgaa ataaaacagt cgatcgcaga ctatgcggtt cttaagcatg
     3601 cggaatatgg tgaagtatat gcttatgaag tagacggcta cggacaatac aatcttatgg
     3661 atgatgcgaa tgttccgagt ttgctgtcca tgccgtatct cggattttgc agggaaaatg
     3721 atgagatcta tcaaaacacc agaaggatga ttttaagcga gaccaatcca ttctattttg
     3781 agggaaaagc attaacggga atcggaagtc cgcatatacc tggaaagtat gtatggcata
     3841 tatccaaggc aatggaagga ctgacctgta cggaccggac gaggaaaaga gagatcatcg
     3901 agcagctgaa aaatacggat gccggcacct ttatgatgca tgagggcgta tatgcggatg
     3961 atgccgcgca atatacgaga gcgtggtttt cctgggcaaa tgcaatgttc agcgaactgg
     4021 tgctgtcgta ttgcggatat gaggtcatac gataaaagaa gaaggggctg ttctcctgtg
     4081 ttttcggaga aacagcctct ttttaaaccc tttcattcag tccagcctgt cagcctgttt
     4141 ttcaaaagcg gtcagatgat ttttagaagc gtccattaag gaagtaaata ctttttccac
     4201 atcggccggc aggtcatagg tcagaaacag ttcatacatt tcaatattat tgatttcggc
     4261 cgtaacaccg gtctgtgctg cctgcagaag cgttgcagca ggtatggtgt aagaagagct
     4321 gagatcttct ggaacaggga tatcataagt ttcaaacaga gttgtcagcg aagaaatgtg
     4381 agtttcttcc gcctgtatga tatttgtgta cggattgttg tttccatacg cggcaatgat
     4441 ctgttcatat tccgcatgtg caagagattc atcctggata gcataagtaa gcatttccct
     4501 aagagtcaga tcttcttcct gcagggcgcc tgccgctccg tatgagctgc tgtctgcgga
     4561 gtcagaagct tgtaactcgt cagagactgt gacggaagag tttccggtat ttttattacc
     4621 ggttccccgg ccatttgaca ggccgctttt ggtaccctgg ttttgtctga cggaggcgtt
     4681 tacggtttcc tgactgcagg cagacaggga aattgtcagt actatgaggg tgatgaaagc
     4741 tgcggcaagt gtttttttac ggttcgtttg ttttttcata atggatgctc ctttcctttt
     4801 ctttgctttt atagtattct gaacctgtgg cagaattgtg acaaaaacag gacaagaatc
     4861 gtacgtttta cgcgtcaggc agtgcgaagg caggaaaata tgctaaaata aaagcaaagt
     4921 atcgccaaag agaaggagag aggcttatga aaatgctgat agccgacgac aacaggcaga
     4981 tcgtatcgat tttaacggaa tacgggaaaa aggaagggtt tgaaacagtg cctgcctatg
     5041 acggaaaaca ggcgctggag ctggcactga caggaaactt tgatatgatc cttctggatg
     5101 tatccatgcc cgtgatggat ggatttgaag tatgcaggca gatcagacgc agtttaagtg
     5161 ttccggtgat catgataacg gcaaggggag aggattttga aaagatcatg gggctggata
     5221 tcggtgcgga tgattatatt ataaaaccgt tttctccagg ggaggttatg gcaagagtgc
     5281 gtgcgatcct gcgcaggata gaaaaaagca cggtatcaga cgggcaggta tggaaccggc
     5341 agggacttta tttaagcctt gatgattata cggtcaggat caacggaaaa gaggtcggcc
     5401 tgacaaaaaa ggaaatggaa ctgctttgga cgcttgcggt gaacagcaat aaagtatttt
     5461 caagggagaa tctgcttaac agcgtatggg gctatgatta ttttggtgat ctgcgtacgg
     5521 tagatacaca tataaaaaga ctgcgtgcaa agctggatcg gtttgaacac agcggctttg
     5581 agctccgaac gatctgggga gtcggatata agcttgaaat catgcaggga gacggcgtat
     5641 gaaaaaaata tcgtggaagc atcaattaac agttcgtctg gtaggctatt ttttccttgt
     5701 atttcttgtt ttctccgtaa tcatcggatt tacgtttttt tattttttcc gggcgaacac
     5761 gatagagctg catcgggccg aattggaaca gcgggctgaa aaaatagcgg agacattagg
     5821 agcaatgtcg gcagatggta ttggaagaaa acaggggaat ggttacgggg catatttgaa
     5881 gtatctggga gatatcgcaa tgtcggatgt atggcttatc gataaggaca gaacgattat
     5941 tacagacggg gacagtgtga acagccatgt ttcgtatacc gatctgccgg aagctgctga
     6001 aaatatgatc gatgaagtgt tcagcggaga aacgctgtat tcttcggatt ttgattcggt
     6061 tctttcagtt cctgtaatta cggtaggaac accggtaatc agcagcacgg gagagattac
     6121 cggcgcgctg ctgctgcatt cgtccgtaga tcatgtcgat ctggcagtca ggaatgcgtt
     6181 tctgatcttt cttttaagca ttacggcagc atttttgatc ggcatgattc tggcggtccg
     6241 tctgtcacga aaatttacaa agccggtatg tatcctgtcc gatattacaa caacgatgtc
     6301 aaaaggcgaa tatggagtac gtgccggatt tgacaggaaa gacgaaatag gccttttagg
     6361 gaattcgata gatattctgg cacagcgtct ggaagagctc gaacggcaga gacagcattt
     6421 gttttcttct gtttcccacg agcttcggac accggtaaca gtcgttcagg gctctctgga
     6481 agcactgatt gacggtgtcg tttccggaga agaccagaaa aaagaatatt tttatcagat
     6541 gcttgcggaa acgaaaaatc tgtccggcct gatcgatgat attttggaat tgtcgaggct
     6601 tcagaaccag gaatttgtgt taacgaaagg agagtgcttt ataaacgaca tcttttccga
     6661 tctaaagaga agtcttgccg tcgtggccaa aagaaagggt gtggggctcg tatttgagca
     6721 ggaatgtccg gcatacggtt tttattgtga ttataacaga atcaggcaga tgttttttgt
     6781 tatttatgac aacgccataa aatattctga tgccgggaaa accgttacga ccacatttca
     6841 gctgatcttt ggcagactgc aggtgacggt ttccgatcag gggatcggaa tcggaaaaga
     6901 ggaacttccg catctgtttg agcggttttt ccggtcggaa cggacaggag acattgcagg
     6961 gagcgggctt ggcctgtcga tcgcagccgg gatcgcaaag cgccatgaca tagagctcag
     7021 tgtaacatca gaagaaggaa agggaacggc attttcattt ttgtttccgg aatcagggga
     7081 aaagaaaagt gctgctggcg gacagaaaca gcatatagta taataatata tacattagtg
     7141 agggggaggt gtttgatttg gaccagatcc ttggatttta tccgttgatt tcgtctatcg
     7201 tgctgctgat gcttgttata acgctttgct tcgtcaatat gtatttactg gccagataca
     7261 gaaaaagttt ttttacgatc atggttcata taggttcgtt ttttgtcgca tccggattga
     7321 ttttttatat agtcgcgcat gcgatttttg cgattccgct gtcacgtttc tatgcaagac
     7381 ttgcagagac ggcattgtgg acggcggtgg tgatatgtgc ggcaggcttt ggatatatag
     7441 ctgtaaatgg aaagaagaac gacttgcggg tactgccgtt cagtattacg gaagtgcttc
     7501 agaatattga agatgtggtg tttgtaattg atacggacgg gatcattacc cacatcaatc
     7561 accctgcgct gtttcagaaa atgtttggcc gaattgacac gctggaacag ctggagaggc
     7621 atctgcaggt gagctgtttt gatacggacc gagcagacgg cgttttgccg gatacgggag
     7681 aactatataa atgtgaagga tattttgaat cgcttaggat gtatgccgcc tttcaaatcg
     7741 caccgatcat gtctttaaaa ggtaagtccg gttatatgat cgtaatagag gatgttacaa
     7801 aagtccggga gagtgaaggc gcgcttttta aacaaaatga aagtttaagg aatgcaaatg
     7861 aacggctcgg cagctatatc aaagcggccg gacagctgga ggctgaaaag gaacggctgg
     7921 taattttaaa acggatccag gaaacgcttt tgcaggatat tgaaggggca ttgtctctta
     7981 ttcaggggat gaaacgcagt gcgtttcagg acgaaagcta tgcaggtgca atgaccggac
     8041 tggcctctgt gctcagatcg acttatcaga aggtgagaaa tgctgtttct cagattgcgg
     8101 gaaaaggaga aaatgtatga ttcgaattgt tatagcagac gatcatctgc tgtttcgttc
     8161 catgctggaa gaaatactga agcgtgatcc gcagctggaa gtgatcggta cgtgtgcgga
     8221 cggcgaagag gtgttggcaa actataaagt ctgggatccg gatctgattc tgctggatat
     8281 cgggatgcca aaaagagggg ggatcgaaac actgaaaacg atcaaagaga aaaccccgca
     8341 gatcaaagtg gtt
//
LOCUS       DAOSYE010000003         7733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6071_length_7733_cov_3.151993, whole genome shotgun sequence.
ACCESSION   DAOSYE010000003 DAOSYE010000000
VERSION     DAOSYE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_7733_cov_3.151993"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PLU43_00070"
     CDS             <1..1024
                     /locus_tag="PLU43_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO26829.1"
                     /translation="LCICMAFLLNGCEFAANDKDNTTLGMEALEALDYSTALDYFEAA
                     LAAGEDQQLVCRGQGMAYLGLAQYTDAITAFEKALAQSDGRVHKIEYDISYYLAVAEY
                     KNGDLDAAYDTYSSILSMNEKDADAWYLRGKVQLAQGEKDAAVADFEQAVELEPENYD
                     LYIKIYEDLAEADDTSDGEAYISRAMENNEKMSDYQVGIFSYYLGNYEDARNYLEKAR
                     ESDDSKELVLYLGKTYEALGDVNYAVSLYTAFLDKDQQAAAVYNQLGLAQMELADYED
                     ALTAFENGIALEDITYMQSLKFNEIVAYEYLTEFKKAAVQMEEYLKLYPDDEKALREY
                     EFLKTR"
     gene            1036..2277
                     /gene="hflX"
                     /locus_tag="PLU43_00075"
     CDS             1036..2277
                     /gene="hflX"
                     /locus_tag="PLU43_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOO26830.1"
                     /translation="MMEETCREKERVILFAVSLSNEEDCRQSLAELSELAQTAGADTI
                     GMLLQNRGEIHPVTYLGSGKLEELDEFVRETKATGVICDDELSPLQFNKLSEILHVKV
                     MDRTLVILDIFAKHASSAEGKLQVELAQLAYTQARLKGFGHAMSRLGGGIGTRGPGEK
                     KLETDRRLINKRMAVLNRELKDVGKHREETRKKRKKNGLFVIALVGYTNAGKSSILNM
                     LTGASVLQEDKVFATLDPTTRIWCLPDKQEVLLTDTVGFIRKLPHHLINAFRSTLEEA
                     RYADIIFHVVDASSPYMDMQMHVVYETLTELGISNKRIVTLFNKQDKIKDEIHVRDLK
                     AEAVLKISAVKREGAEQLGQLMIRLLTEGSMLFERLVPYSEVFVIQKIRKNGRLLKEE
                     YQAEGIYVKAYVPKELYGALV"
     gene            2468..4858
                     /locus_tag="PLU43_00080"
     CDS             2468..4858
                     /locus_tag="PLU43_00080"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOO26831.1"
                     /translation="MFSVVKRDGEIANFDLTKINDAVMKAFVACEKQYNNDIIDLLAL
                     RVTADFQTKMTEGLVHVEDVQDSVETVLEQAGYTDVAKAYILYRKQREKIRNMKSTIL
                     DYKDVVNSYVKVEDWRVKENSTVTYSVGGLILSNSGAVTANYWLSEIYDQEIAEAHRN
                     ADIHIHDLSMLTGYCAGWSLKQLIKEGLGGISGKITSAPASHLSVLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFVKVDDLTYREVKKCIESFVYGVNTPSRWGTQAPFSNITL
                     DWTVPNDLAELPALVGGKEMDFKYKDCKKEMDMINKAFIETMIEGDANGRGFQYPIPT
                     YSITKEFDWLDTENNRLLFEMTSKYGTPYFSNYINSDMEPSDVRSMCCRLRLDLRELR
                     KKTGGFFGSGESTGSVGVVTINMPRIAYLSANKDDFYKKLNRMMDISARSLKIKRGVV
                     SKLLKEGLYPYTKRYLGNFDNHFSTIGLIGMNEVGLNANWLRADMTDKKTQEFTKEVL
                     NHMRNRLSDYQEEYGDLYNLEATPAESTTYRLAKHDKKRWPNIKTAGKPGDTPYYTNS
                     SHLPVDYTSDIFDALDIQDDLQTLYTSGTVFHAFLGEKLPGWSAAAALVKKIAENYKL
                     PYYTLSPTYSVCKQHGYLVGEQKTCPHCGAATEVYSRITGYYRPVQNWNDGKAQEFKN
                     RIVYDIENSSLKRPTATMVTLSGLDNDDEVNVTPVEDVKYLFTTKTCPNCKFAKDYLK
                     DEKYLLIDAEENRELASRYGVMQAPTLVIVNGEEYKKYVNASNIRKYAEEQKEVFV"
     gene            4941..5864
                     /gene="pyrF"
                     /locus_tag="PLU43_00085"
     CDS             4941..5864
                     /gene="pyrF"
                     /locus_tag="PLU43_00085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO26832.1"
                     /translation="MIDKLIANIKKTNAPIVVGLDPMLSYIPAHILKKAFEDYGETLE
                     GAAEAIYRYNTGLVDAIYDLIPAVKPQIAMYEQFGVPGLAAFGRTVDYCREKGLVVIG
                     DIKRGDIGSTSAAYAAGHLGKVQVGGSRISVFNEDFATINPYLGTDGIKPFIDVCKEE
                     QKGLFILVKTSNPSSGEFQDRLIDGRPLYEHVGEHVARWGEECMGEHYSHIGAVVGAT
                     YPRIGKVLRDIMPKAYILVPGYGAQGGEGKDLKPFFNEDGLGAIVNSSRGIITAYKSG
                     RYASYGALNYADASRKAVLDMQKDIAEGVGL"
     gene            5940..6641
                     /gene="pyrE"
                     /locus_tag="PLU43_00090"
     CDS             5940..6641
                     /gene="pyrE"
                     /locus_tag="PLU43_00090"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO26833.1"
                     /translation="MEQYKQEFIEFMVDSDVLKFGEFTLKSGRRSPFFMNAGAYVTGS
                     QLRKLGEYYAKAIHDNYGLDFDILFGPAYKGIPLAVATTMAIHELYGKEIRYCSNRKE
                     VKDHGDTGILLGSGPKDGDRIVMIEDVTTSGKSIAETFPLIKAQADVTIRGLIVSLNR
                     MERGLATSMSALDEIRQNYGFETNAIVSMQEVVAYLYDRPYRGRVLIDRAMKESIDRY
                     YEQYGVKQDGEAQKD"
     gene            6619..6960
                     /locus_tag="PLU43_00095"
     CDS             6619..6960
                     /locus_tag="PLU43_00095"
                     /inference="COORDINATES: protein motif:HMM:NF039667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6142 family protein"
                     /protein_id="HOO26834.1"
                     /translation="MAKPKKIKKRKSFMFTSRHQSEIGIMSFVLGAASFTGMAAAVSV
                     SFAARGETAPGLGAVGFFAALANIIGIVAGILSLQERDIFLWLPRTALIMNILALALW
                     IVLIILGVRGV"
     gene            6962..>7733
                     /locus_tag="PLU43_00100"
     CDS             6962..>7733
                     /locus_tag="PLU43_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOO26835.1"
                     /translation="MASERYQLSIDRIREILEEETVKEPFLSCFKKTASFFILLETVS
                     ELEEDGSLCKLSIEKLRDLNDRLYEDILPGNYEGSCANPTYMEKLCKQYGCSKKYGQY
                     FSFLYTEIRGLIPEAFEGHRDIMTLYQELFVEIYDLFVFAQADALVTESDGMKQNLPK
                     LEELHQSIYWFESDNCDILVPKRLDEQLDPKCDFAAGLVMNSDLEDLRYLYLFGEYIT
                     ENECRIAAYLNGMPKEQIDAMASTYTEGYRIGFVKGNKD"
BASE COUNT         2280 a         1359 c         2069 g         2025 t
ORIGIN      
        1 actttgtata tgcatggcgt ttctgttaaa cggctgtgaa tttgctgcaa atgacaaaga
       61 taataccaca cttggcatgg aggcgcttga ggcgcttgat tacagcacgg ctttggatta
      121 ttttgaagct gcgcttgcag ccggcgaaga tcagcagctt gtctgcaggg gccagggaat
      181 ggcatattta ggtcttgcgc agtatacgga tgcaattacg gcatttgaaa aggcacttgc
      241 gcagtccgac ggcagggtgc ataagatcga atatgatatc tcctattatc ttgcggttgc
      301 cgaatataaa aacggagacc tggatgcggc atatgatacc tattcttcta ttctttccat
      361 gaatgaaaag gatgcggacg cctggtacct gcgtggaaag gtacagctgg cacagggaga
      421 aaaagacgct gcggtcgctg attttgaaca ggctgtggaa ctggaaccgg aaaattatga
      481 tctgtatatc aaaatttatg aagacctggc agaagcagat gatacaagcg acggagaagc
      541 ttatatcagc cgggccatgg aaaataatga aaagatgagt gattatcagg tcggtatttt
      601 cagctattat ttaggcaact atgaggatgc aagaaattat ctggaaaaag cgcgggaatc
      661 agatgattca aaagagcttg ttctgtatct tggaaagact tatgaggcgc ttggcgatgt
      721 caattatgca gtttctcttt atacggcatt tttagataaa gatcagcagg ctgccgcagt
      781 ttataatcaa ctggggcttg cgcagatgga gcttgcggat tatgaagatg cgctgactgc
      841 ttttgaaaac gggatcgcat tggaagatat tacttatatg cagagtctga aatttaatga
      901 aatagtggct tatgagtatc tgaccgagtt caaaaaggcg gcagtacaga tggaagaata
      961 tctgaagctg tatcctgatg atgaaaaagc gctgcgggaa tatgaatttt taaaaacaag
     1021 ataagggatg cagatatgat ggaagagacg tgcagggaaa aagaacgcgt gattttgttt
     1081 gcggtcagtc tgtcgaatga ggaagactgc aggcagagtc ttgcagaact gtctgagctt
     1141 gcgcagactg ccggtgccga tacgatcggg atgcttttac agaacagggg agaaattcat
     1201 ccggttacat atctggggag cggaaaactt gaggaattag atgagtttgt aagggaaaca
     1261 aaagcaacag gtgttatttg cgatgatgaa ctatctccgc tgcaatttaa taaattgtcc
     1321 gagatactgc atgtcaaagt gatggacaga acacttgtta ttcttgatat ttttgcaaaa
     1381 catgcgagca gcgccgaagg gaagctgcag gtcgagcttg cacagcttgc ttatacacag
     1441 gccaggctca aaggattcgg acatgccatg tcaagactgg gcggcgggat cggaacaaga
     1501 ggtcccggtg aaaaaaaact ggaaacggac agacgtctga tcaataagcg aatggcagtg
     1561 ctgaacaggg agcttaagga tgttgggaag caccgtgagg aaacgagaaa aaagagaaag
     1621 aaaaatgggc tttttgtcat tgcccttgtc ggatatacga atgcggggaa atcctccata
     1681 ttaaatatgc tgaccggagc ttccgtatta caggaagaca aagtatttgc cacactggat
     1741 ccgactaccc gtatctggtg tcttccggat aagcaggagg tgctcttaac ggatacggtc
     1801 ggttttatca gaaaactgcc gcatcatctg atcaatgcgt ttcgaagtac gctggaagag
     1861 gccagatatg cggatattat ttttcatgtt gtcgatgcct caagtcctta tatggacatg
     1921 cagatgcatg tagtttatga gacgttaaca gagcttggga tctccaataa aagaatcgtt
     1981 acactcttta ataaacagga taaaataaaa gatgaaatac acgtcaggga cctgaaggcg
     2041 gaggcagtgc tgaaaatttc ggcagtgaag cgggaaggtg cggagcagct gggccagctt
     2101 atgatcagac tgctgaccga gggcagcatg ctttttgaga gactggttcc ttattcggaa
     2161 gtttttgtta ttcagaaaat cagaaagaac ggcagactgc tgaaagagga ataccaggcg
     2221 gaaggaattt acgtaaaggc ctatgtacca aaggagcttt acggagcgct tgtataggtg
     2281 cgcatactat atcttgtgct attaatgaaa cactgacata atatatggtg ttttttgttg
     2341 acttttctat tttgcgatgc tataattttt ttatagttgg tttttagtca ctcggcaggg
     2401 gaatcagaat ttgccgagtg atttgcgcca aacagcagaa tatataaaaa gggaagggag
     2461 taatgggatg tttagtgtag taaaaagaga tggggaaatt gcaaattttg atctgacaaa
     2521 gatcaacgac gcggttatga aagcattcgt tgcgtgcgag aagcagtata acaatgatat
     2581 aatcgatctt ctggcgctga gagtcacggc ggattttcag acgaagatga cagagggtct
     2641 tgttcatgtg gaagacgtgc aggacagtgt ggagaccgta cttgagcagg ctggctatac
     2701 ggatgtggca aaggcatata tcctttatcg taaacaaaga gaaaaaattc gtaatatgaa
     2761 gtccaccatt cttgactata aggatgttgt aaacagttat gtaaaagtcg aggactggcg
     2821 tgtaaaagag aattctacgg tcacctattc tgtaggaggc ctgatcttaa gtaattccgg
     2881 agccgtaacg gctaattact ggctgtctga gatctatgat caggaaatcg ccgaggcaca
     2941 cagaaatgcg gatatccata ttcatgatct gtccatgctg acaggctact gcgcgggctg
     3001 gtctttaaag cagctgatca aggaaggact tggaggcatc agcggcaaga tcacatcagc
     3061 accggcgtct catctttctg tgctttgtaa ccagatggtc aattttctcg gcattatgca
     3121 gaatgaatgg gcaggtgcac aggctttttc ttcgtttgac acctatcttg ccccgtttgt
     3181 aaaggtggat gaccttactt acagggaagt gaaaaaatgt atcgagtcat ttgtatatgg
     3241 agtgaatacg ccaagccgct ggggcacaca ggcaccgttc tcaaatatta cgcttgactg
     3301 gacggttccg aatgatcttg cagagcttcc ggcacttgtc ggcggcaagg agatggattt
     3361 caaatataag gactgtaaaa aagaaatgga tatgatcaac aaggcgttta ttgagacaat
     3421 gatcgaagga gacgccaatg gacggggctt ccagtatccg atccccacgt attccattac
     3481 aaaggaattt gactggctcg acacggaaaa caatcgtctg ctgtttgaga tgacttccaa
     3541 atatggaacg ccgtattttt cgaattatat caacagtgac atggagccga gtgatgtcag
     3601 gagcatgtgc tgcagacttc gtcttgatct aagagagctt cgcaagaaga ccggcggatt
     3661 tttcggttca ggcgagagca cgggttctgt tggcgtcgtt acgatcaata tgccgcgtat
     3721 tgcatatctt tccgccaata aagacgattt ttataaaaaa ctgaatagga tgatggacat
     3781 ttctgcccgc agcttaaaaa tcaaaagagg ggttgtttca aagcttttga aagaagggct
     3841 atatccttat acgaaacggt atcttggtaa ttttgacaat catttttcca cgatcggtct
     3901 gatcggcatg aatgaggtcg gtttaaatgc gaactggctg cgtgcggata tgacagacaa
     3961 aaaaacacag gaattcacaa aggaagtact caaccatatg agaaaccgcc tttctgatta
     4021 tcaggaggag tacggggatc tgtacaacct tgaagcgaca cctgccgaaa gtacgactta
     4081 ccgtctggca aagcatgaca agaagcgctg gccgaatatc aaaacggcag gcaagccggg
     4141 agatacaccg tattatacaa acagttctca tctgccggtg gattatacat ctgatatttt
     4201 cgatgcgctc gatattcagg atgatctgca gacattatat acgtccggta ccgtatttca
     4261 tgcgttcctc ggagaaaagc ttcccggctg gagtgctgcc gctgcgcttg tcaaaaagat
     4321 cgctgaaaat tataaactgc cgtattatac gttatccccg acttattcgg tatgtaagca
     4381 gcatggatat cttgtagggg aacagaagac ctgtccgcat tgcggagcag cgaccgaagt
     4441 ttacagcagg attacaggat attacaggcc ggtgcagaac tggaatgacg gcaaggcgca
     4501 ggagtttaag aacaggatcg tttacgatat tgaaaattcc agcctgaaaa gaccgacagc
     4561 aacaatggtg actttgtcag gacttgataa tgacgatgaa gtcaacgtta cgccggtgga
     4621 ggatgttaaa tatctgttca cgacgaagac ctgtccaaac tgcaagtttg caaaggacta
     4681 tttgaaagat gaaaagtatc ttttgatcga tgcggaagaa aaccgggaac ttgcttccag
     4741 gtatggggtc atgcaggcac cgactctcgt tattgtgaac ggagaggaat ataaaaagta
     4801 tgtgaacgct tccaatatta gaaaatatgc cgaagagcag aaggaagtat tcgtatagac
     4861 catggattac tgattgatta ataaagaagg agatgcggtt aagcatttcc ttcttttggg
     4921 aaaagaagga gaatggtgaa atgatcgata aattaattgc caatatcaaa aaaacgaatg
     4981 ccccgatcgt agtgggactt gatccaatgc tttcttatat accggcgcat attttaaaaa
     5041 aagcgtttga agattacggg gaaaccctgg aaggggcagc ggaagctatt taccgttata
     5101 atacgggact tgtggatgcc atatatgatt tgattccggc agtaaaaccg cagatcgcga
     5161 tgtatgaaca atttggggtg ccgggactgg ctgcttttgg aaggacagtg gattactgca
     5221 gggaaaaggg tcttgttgtg atcggagata tcaagcgggg tgatattggt tcgacttccg
     5281 ctgcctatgc ggcaggacat ttggggaagg tgcaggtagg aggcagcagg atatcggtat
     5341 ttaacgaaga ctttgcaacg atcaatccat atcttgggac ggatgggatc aaaccgttta
     5401 tagatgtctg caaagaagaa cagaaaggac tgtttattct tgtgaaaacg tccaatccga
     5461 gcagcgggga attccaggac aggctgatcg acggacgccc gctttatgaa cacgtgggag
     5521 agcatgtcgc aagatggggt gaggagtgca tgggagagca ttacagtcat atcggagctg
     5581 tggtcggtgc tacttatcca aggatcggta aggttttgcg tgacattatg ccaaaagcct
     5641 atattctggt acctggctat ggagcgcagg gcggagaagg aaaggatctg aaaccatttt
     5701 ttaatgaaga tggtctcgga gcgatcgtta attcctccag aggtattatt accgcataca
     5761 aatcggggag atatgcgtcc tatggagccc tgaattatgc ggatgccagc cggaaagccg
     5821 tgcttgacat gcaaaaggat attgcggaag gcgttggtct gtagaatata attgctgatc
     5881 aattggtagt atgatataat gtgtgaggct gcggacggtt tttaaggaga aaaaagcgga
     5941 tggaacagta taagcaggaa tttattgaat ttatggtaga cagcgatgtg ctgaaatttg
     6001 gagagtttac gcttaaaagc ggcagaagat ctccattttt tatgaatgcc ggagcgtatg
     6061 tgaccggttc acagcttagg aaacttggcg aatattatgc gaaagcgatc catgataatt
     6121 acggactgga ttttgacata ctgttcgggc cggcatataa ggggattccg ctggcggttg
     6181 cgacaacgat ggcgatccat gaattatatg gaaaggaaat cagatactgt tctaacagaa
     6241 aagaagtaaa ggatcatggg gatacgggta ttcttcttgg cagcgggcca aaagacggcg
     6301 acaggatcgt tatgatcgag gatgtgacta cttcagggaa atccattgca gagacatttc
     6361 cgctcatcaa agcgcaggcg gatgtgacga tcagaggact gatcgtatct ttgaaccgta
     6421 tggaacgggg attggccaca tcaatgagtg cacttgacga gatcaggcag aactatggat
     6481 ttgagacgaa tgcgatcgtt tccatgcagg aggtcgttgc atatctgtac gatcgtccgt
     6541 atcgcggcag agtgctgatc gaccgggcaa tgaaggaatc tattgacaga tattatgagc
     6601 agtatggagt gaagcaggat ggcgaagccc aaaaagatta aaaagagaaa aagctttatg
     6661 tttacttcaa ggcaccagtc tgaaatcggg atcatgtcat ttgtgcttgg agcagcatct
     6721 tttacgggga tggcggcagc tgtttccgtt tcttttgcgg cacgcggcga aacagcacca
     6781 ggtcttggcg cagtcggttt ttttgccgca cttgcaaata taattggaat tgtagcaggt
     6841 atattgagtt tgcaggagag agatatcttt ttgtggcttc cgcggaccgc actgattatg
     6901 aatatacttg cgcttgcttt atggatcgtt ttaattattt taggtgttcg aggtgtatag
     6961 tatggcgtca gaaagatatc agctttctat tgacagaatc agagaaattt tagaggaaga
     7021 aacggtaaag gagccttttt tgtcatgctt caaaaaaacg gcttctttct ttattttatt
     7081 ggaaacagtc agtgaacttg aggaagatgg atcattatgt aaacttagta ttgaaaaact
     7141 gagggatctc aatgatagat tatatgagga tattttgccg ggaaattatg agggtagctg
     7201 tgcgaatcca acgtatatgg aaaagttatg taaacaatat ggctgttcga aaaaatatgg
     7261 acagtatttt tcatttcttt atacggaaat ccgtggtctg ataccggaag catttgaagg
     7321 ccatcgggat attatgacac tttatcagga gctttttgtt gaaatttatg atctgtttgt
     7381 ttttgcacag gcagatgcat tggtgacaga gtcagacggg atgaaacaaa atcttccaaa
     7441 actggaggag cttcatcaga gtatttactg gtttgagagt gataactgcg atatcctggt
     7501 tccgaagaga cttgacgagc agcttgatcc aaagtgtgat tttgcagctg gtctggtcat
     7561 gaattccgat ttggaagatc tgcgttattt gtatttgttc ggtgaatata ttacggaaaa
     7621 tgaatgcagg atcgctgctt atctgaatgg aatgccaaag gaacagatcg atgcgatggc
     7681 gtcgacttat acggaggggt atcgtattgg ctttgtaaaa ggaaataagg atc
//
LOCUS       DAOSYE010000004         7680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6149_length_7680_cov_2.752131, whole genome shotgun sequence.
ACCESSION   DAOSYE010000004 DAOSYE010000000
VERSION     DAOSYE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_7680_cov_2.752131"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PLU43_00105"
     CDS             <1..1225
                     /locus_tag="PLU43_00105"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOO26836.1"
                     /translation="EIESGWYHWEDIMHHSESLKRDYVRIFEEMLPQIASTYAPQTFY
                     WRSSPSSGGGFDNPDDETRGDCHYWDVWHGQKPFSAYKDHYMRFCSEFGFQSFPSIKT
                     VKTFTREEDRNIFSEVMESHQKNTGANGKLLYYLSENFLYPKDFESLLYVTQILQAMA
                     VKYGVEHWRRQRGRCMGTLYWQLNDNWPVASWSSIDYFGRWKALQYMAKNFYSQIAGS
                     IERTGSTASVYIQNESRDYESRRVKISLQTLDFRLLHEAEYEVEIKPFEALKVCENDY
                     APYITGIENRVFMEAVFIDEDDTVVSSEIEIFVPYKRLALGTSSISYSVIELADEYVI
                     RMMSGGFAAFVELDLKQADAVFSENYFHLTSKREKAIQLKKTDIRYLKHGEPEIQNGY
                     ELEQQLVIRTLRDTY"
     gene            1279..2289
                     /locus_tag="PLU43_00110"
     CDS             1279..2289
                     /locus_tag="PLU43_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO26837.1"
                     /translation="MAKAVKLADIAKRVGVSTVSVSKALSGKGGVSSQVQEQIEQVAK
                     ELGYVSKADAKKRKSGSTGNIGVLLPQRFVGQNTFYWGLYQEIVNALALRGFYGILEL
                     LSFEDEKNQVIPKMILDKKVDGVIVLGQARAEFTTFLYDLKAVPVIFLDYYVGKEEYD
                     TVISDGFHGMYRLTNYLIDMGHREIGFVGTTLATSSITDRYLGYIKALMEAGIEPKTE
                     WLIPDRELGTDTLVDLVLPKTLPTAFACNCDVIAYRLLRKLKERGLSVPEDISIVGYD
                     NYAYEDDAQDPVTTYDVGMRRMAETCVRALVSKIYGDRYYKGVQIVTGHIVYKKSVRR
                     IR"
     gene            2299..2478
                     /locus_tag="PLU43_00115"
     CDS             2299..2478
                     /locus_tag="PLU43_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HOO26838.1"
                     /translation="MAEIPKDPVMLLSFVNLKLRDRYKNPDELCEDLGIDRQALTDRL
                     LEIDYFYDEKSNQFR"
     gene            2510..3838
                     /locus_tag="PLU43_00120"
     CDS             2510..3838
                     /locus_tag="PLU43_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO26839.1"
                     /translation="MDLFAYMRENHMEKESPLAARLRPDTLEEVVGQRHIIGKDKLLY
                     RAIKADKLSSLIFYGPPGTGKTTLAKVIAHTTSAEFTQLNATTAGKKDMEAVISQAKD
                     MLGMYGKKTILFIDEIHRFNKGQQDYLLPFVEDGTVVLIGATTENPYFEVNGALLSRS
                     VIFELKPLEKEDIRTLLTRAVYDKEKGMGAYDAVIDEDALEFLSDISGGDARAALNAV
                     ELGIMTTQRAADGKIHITSEVASECIQKRVVRYDKTGDNHYDTVSAFIKSMRGPDPDA
                     AVFYLAKMLYAGEPVTFIARRIMICASEDVGNADPMALTVAVAAAQAVERIGMPESQL
                     ILSQAVTYIATAPKSNAATQAIGEAMKAVETAKDMTIPVHLQDAHYKGAAKLGHGEGY
                     QYAHDYPNHYVEQQYLPYELSGREFYHPSGNGYEKKIREHMKWLKEQASQ"
     gene            complement(3830..4342)
                     /locus_tag="PLU43_00125"
     CDS             complement(3830..4342)
                     /locus_tag="PLU43_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOO26840.1"
                     /translation="MTDPLTLYKLIVLYMLDQVDFPLTKAQIFDFILEKEYTNYFTLQ
                     QVTCELIDTGLVESNTLRNSSHLKITEEGRNTLYYFKGRISDGIKADIHTFFRDNELE
                     LRNEVSIQSSYYKATTGEYVAELTAKETHTDLLNIKITMPTEEAASNICENWQKKNQD
                     IYAFLMENLL"
     gene            complement(4374..5306)
                     /locus_tag="PLU43_00130"
     CDS             complement(4374..5306)
                     /locus_tag="PLU43_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248094.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOO26841.1"
                     /translation="MQKQWGQKSYYSFDYYVKSHFGQKLYKLSLNGHMTCPNRDGTLD
                     TRGCIFCSEGGSGEFTPSYLTSVTAQIEEAKKLISGKYHSDRYIAYFQAYTNTYAPVP
                     RLRKLYMEAVLHPDIAALSIATRPDCLGGDVLALLAEINRIKPVFVELGLQTIHEDTA
                     VFIRRGYPLARFTDAVTKLHALAIPVVVHLILGLPGETPAQMLSTVQYMNMLPISGVK
                     LQLLHVLKHTTLASLYEAGLYTPLTQEAYTSILIDCIGHLCESIAIHRITGDAPKQLL
                     IAPLWSADKKKVLNNIHKELKKQQIWQGKYLTQN"
     gene            5515..6882
                     /gene="rho"
                     /locus_tag="PLU43_00135"
     CDS             5515..6882
                     /gene="rho"
                     /locus_tag="PLU43_00135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO26842.1"
                     /translation="MTREKYESLSLASLKGIAKARGIKNVSAMKKAEVVEIMLAEDEK
                     ETGNEENKPAEKTKSSAQNNSSLSVDIDISEEKKCLDSGIEANGILEVLTEGYGFIRS
                     ENYLPGENDVYVAPSQIRRFGLKTGDIIVGNTRVKSQNDKFGALLFVKLINGMTPQEA
                     SKRKNFEDLVPIFPDERLKLETQGGSIAMRIMDLMSPVGKGQRGMIVSPPKAGKTTLL
                     KDVAKSIKRNNPEMHLIILLIDERPEEVTDIKEAIEGPNVEVIYSTFDELPEHHKRVS
                     EMVIERAKRLVEHGRDVIILLDSITRLTRAYNLTVPPSGRTLSGGLDPAALHMPKRFF
                     GAARNMRGGGSLTILATALIETGSKMDDVVYEEFKGTGNMEMVLDRKLSERRVFPAID
                     IPRSGTRREDLLLDREELEAINVMRKALNGLKPEEAVEKILDMFVRTRNNREFVDMVK
                     KIRFI"
     gene            6981..7187
                     /gene="rpmE"
                     /locus_tag="PLU43_00140"
     CDS             6981..7187
                     /gene="rpmE"
                     /locus_tag="PLU43_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOO26843.1"
                     /translation="MKEGIHPKYYQATVTCNCGNTFVTGSTKEDIHVEICSKCHSFYT
                     GQQKSVRADGRIDKFNRKYGVESK"
     gene            7250..>7680
                     /locus_tag="PLU43_00145"
     CDS             7250..>7680
                     /locus_tag="PLU43_00145"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOO26844.1"
                     /translation="MAKRKKRAKKDHYSGIGGQAVIEGIMMKNKDKYSVAVRTQSNEI
                     EVVLEEYESFAPSERLTQIPFIRGVFNFIDSMILGIRTLNYSASFYEEVDEKDKKKQK
                     PADKMLGDNSEKIMTGLTVFLSIVIAVGLFMLLPYFLSQLL"
BASE COUNT         2154 a         1482 c         2072 g         1972 t
ORIGIN      
        1 tgaaatagaa tcgggctggt atcactggga ggatatcatg catcattccg aatcgctgaa
       61 acgggattac gtcaggatct ttgaagagat gcttccgcag atcgcctcga cgtatgcgcc
      121 gcagactttt tactggcgtt cttccccgtc ttccggggga ggctttgaca atccggatga
      181 tgagacaaga ggggactgtc attactggga tgtatggcat ggacaaaaac ctttttccgc
      241 atacaaagac cattatatga gattttgttc ggagtttggc tttcagtcgt ttccctcgat
      301 caaaacggtc aaaacgttta caagggaaga ggacaggaat attttttcag aggtcatgga
      361 gagccatcag aaaaacactg gggcaaatgg aaaactgctg tattatcttt ccgaaaactt
      421 tttgtatccg aaggattttg aaagtctgct ttatgtgacg cagatcctgc aggccatggc
      481 agtcaaatat ggagtggaac actggcgcag acagcgcgga cgctgtatgg ggactcttta
      541 ctggcagctg aatgataact ggccggttgc ttcctggtcc agtatcgact atttcggtcg
      601 ctggaaggcg ctgcagtata tggcaaagaa tttttattca cagatcgccg gttccattga
      661 acggacaggc agtacggcat cagtctacat ccagaatgaa tcgcgcgatt atgagagcag
      721 acgtgtaaag atcagcctgc agacgctgga tttccggctg ctgcatgaag cggaatacga
      781 ggtggagatc aagccgtttg aagcgctgaa ggtatgtgaa aatgactatg caccgtatat
      841 tacgggaatt gaaaacaggg tgtttatgga agcggtattt attgatgagg atgatactgt
      901 tgtttcctca gagatcgaga tctttgttcc gtataagcgt ctggcgcttg gaacgtcgtc
      961 tatttcctat tctgtaatag agcttgcgga cgagtatgtg atccgtatga tgtcaggcgg
     1021 gtttgctgct tttgtagagc ttgacctgaa gcaggcagat gcggtattca gtgagaatta
     1081 tttccacctg acaagcaaac gggaaaaggc gatccagctg aaaaaaacag atatccgtta
     1141 tttaaaacat ggggaaccgg agatccaaaa cggatatgag ctggaacagc agcttgtgat
     1201 caggacactg cgtgatacct attaaggaca ggcggattaa agcataagaa tgacagatga
     1261 aaagaggaac gggagattat ggcgaaagca gtgaaattgg cggatattgc gaagagagtc
     1321 ggtgtcagca ccgtatccgt ttccaaggct ctttcgggaa agggaggcgt gagcagccag
     1381 gtacaggagc agatcgaaca ggtggctaag gagcttggct acgtttccaa agcagatgca
     1441 aagaaaagaa agagcggcag tacggggaat atcggagtat tgctgccgca gcggttcgta
     1501 ggccagaata cgttttactg gggattgtac caggagatcg tcaacgccct tgccctccgc
     1561 gggttttatg gtattttgga gctgctttcc tttgaggatg aaaaaaatca ggtgattccc
     1621 aaaatgatac tggataaaaa ggtggacggc gtgatcgtct taggccaggc acgggcggaa
     1681 ttcacgacat ttctctacga tctgaaggct gttcctgtga tctttttgga ctattatgtt
     1741 gggaaagaag aatatgacac tgttatatcg gatgggtttc acggcatgta ccgcctgacg
     1801 aattatctga tcgacatggg tcatcgtgag attggatttg tcggtaccac gcttgcaacg
     1861 agcagtatta cggacagata tctggggtat atcaaggcgc tgatggaagc cggcattgag
     1921 ccgaaaacgg aatggctgat tccggacagg gagcttggta cggatacact tgtggatctg
     1981 gtgctgccca aaacgctgcc gaccgcattt gcctgcaact gtgatgtgat cgcttaccgg
     2041 ctgcttcgga agctgaagga gcgggggctg tccgtaccgg aagatatttc gatcgtcggt
     2101 tatgataatt atgcctatga ggatgatgcg caggatccgg tgacgactta cgatgtcggt
     2161 atgcgccgaa tggcggaaac gtgcgtgcgt gcacttgtga gcaagatcta cggagaccgt
     2221 tattataaag gtgtccagat cgtgacgggg catatcgtat acaaaaagag tgtcaggaga
     2281 atcagatagg agaagtttat ggcagagata ccaaaggatc cggtcatgct tttaagtttt
     2341 gtgaatctaa agcttaggga tcgttataaa aatccggatg aattgtgtga agaccttggg
     2401 atcgacaggc aggcactgac ggaccgtctt ttggagatcg attattttta tgatgagaaa
     2461 agcaatcagt tccgctgaca gcgggtgctt tgcggatagg gaagatcaga tggatttgtt
     2521 tgcatatatg agagaaaatc atatggagaa agaatcgccg ctggcagcca gactgcgtcc
     2581 ggatacactg gaggaggtag tcggccagcg gcatattatc gggaaagaca agctgcttta
     2641 cagggcgatc aaagcggaca agctaagttc acttatcttt tacggaccgc cgggcaccgg
     2701 aaagacaacg cttgccaagg tgatcgcaca cacgacaagt gcggagttta cgcagctgaa
     2761 tgcgacgact gccggaaaaa aggatatgga ggcagttatt tcccaggcaa aggatatgct
     2821 tgggatgtac ggaaaaaaga cgatcctgtt cattgacgag atccatcggt tcaacaaagg
     2881 gcagcaggat tacctgcttc cgtttgtcga agacggaaca gtcgtgctga tcggcgctac
     2941 aacggaaaat ccttattttg aagtgaacgg tgcgctgttg tcccgttccg tgatatttga
     3001 gctgaagcct ttggaaaaag aagatatcag aacactattg accagagcgg tttatgataa
     3061 agaaaagggc atgggagcct atgacgcggt cattgatgag gatgcactgg aatttttgtc
     3121 cgatatttca ggaggggatg ccagagcggc actcaatgcg gtcgagcttg gcattatgac
     3181 gacacagcgc gcagccgacg ggaaaatcca tattacatcg gaggtcgctt ccgaatgtat
     3241 tcagaaacgg gtcgtcaggt atgataaaac aggtgacaat cactatgata cggtttcggc
     3301 atttattaag agcatgcgtg gtcccgatcc ggatgcggca gtattttatc tggcaaaaat
     3361 gctgtatgcg ggagagccgg ttacgtttat tgcccgcagg atcatgatct gcgcgtcgga
     3421 agatgttgga aatgcggatc cgatggcatt gaccgtggcg gttgccgccg cacaggctgt
     3481 ggagcggatc gggatgccgg aatcgcagct cattttatcg caggcggtca cgtatattgc
     3541 cacggctcca aagagcaatg cggcgacaca ggcgatcggc gaggcgatga aagcggtaga
     3601 gacagcaaaa gacatgacga ttcccgtgca tttacaggat gcgcattata aaggtgccgc
     3661 aaaactcggg catggcgaag gctatcagta tgcgcatgat tatccgaacc attacgtaga
     3721 acagcagtat cttccgtatg aactaagcgg cagggaattt tatcatccgt cgggaaacgg
     3781 ttatgaaaaa aagatcagag agcatatgaa atggctgaaa gaacaggcat cacagtaagt
     3841 tttccatcag gaaagcgtag atatcctgat tctttttctg ccagttttcg catatgttgg
     3901 atgcagcttc ttccgtaggc attgtgatct tgatattgag cagatcggta tgtgtttcct
     3961 tggccgtcag ttccgctacg tattctccgg tcgtagcttt gtaatagctg gactggatgg
     4021 atacttcatt gcgcagctcc agttcattat cccggaagaa tgtatggata tcggctttga
     4081 tcccatctga aatacgccct ttaaaataat aaagggtatt gcggccttct tcggttattt
     4141 tcagatgaga gctgttgcgc agtgtatttg actcgacaag tcctgtgtcg atcagctcgc
     4201 aggtcacttg ctgcagtgta aaataattgg tatattcttt ttcaagtata aaatcaaaga
     4261 tttgcgcttt tgtaagcgga aaatccacct gatcgagcat ataaagaacg atcagcttat
     4321 agagtgtcaa aggatccgtc ataattttcc cacctcctgt agtttctata tgcttaattt
     4381 tgtgtcaggt atttaccctg ccagatctgc tgcttcttca gctctttatg tatgttgttc
     4441 agaacttttt tcttgtcggc gctccataag ggcgctatca acagctgctt cggtgcgtcg
     4501 ccggtaatcc ggtgaattgc gatgctttca caaagatgcc cgatgcagtc gatgaggata
     4561 ctggtgtagg cttcctgcgt cagcggtgta tagagtccgg cttcgtagag ggaagcaagt
     4621 gtggtgtgtt tcagtacatg cagaagctgg agcttgacgc cggatattgg cagcatattc
     4681 atgtattgca cggtggaaag catttgtgcc ggtgtttcgc ccggcaggcc gagtatcaga
     4741 tgtacgacga ccggaatggc cagggcatgc agctttgtga ccgcgtccgt aaagcgggcc
     4801 agaggatatc ctcttctgat aaaaacagcg gtatcttcat ggatcgtctg cagtccgagt
     4861 tccacaaata ccggtttgat acgattgatt tccgcaagca gtgccagaac atcaccgcca
     4921 agacagtcag gccgtgtggc aatggataat gcggcgatgt caggatgcaa aacagcttcc
     4981 atatataatt ttcttaaccg gggaacgggt gcatatgtat tcgtatatgc ctgaaaatag
     5041 gcgatatatc gatcggaatg atatttgcct gaaataagtt ttttggcttc ctcgatctga
     5101 gcggtgaccg acgtaaggta cgacggcgtg aattctccgc tgcctccctc gctgcaaaaa
     5161 atacaaccac gcgtatcaag cgtaccgtct ctgttcggac aggtcatatg tccgttcaaa
     5221 gaaagcttat atagtttttg accaaaatga ctctttacat aataatcgaa agaataatat
     5281 gacttttgac cccattgttt ttgcatttgc atgtagtttc ccctctttct atcataaatg
     5341 attagaggga tgaaatcaag attgttgttg cttttttaaa aataatcatg tataatagca
     5401 aatggttgga aatacatatt tttacgattt tgtttcaaat ccagatataa tcccaataat
     5461 tgatgataga tatccattat aattattcat gtactcttgt aggagggaga aagtatgacg
     5521 cgtgaaaagt atgagtcttt gtcacttgcg tccttgaagg gcattgcgaa ggcacgggga
     5581 attaaaaatg tctctgccat gaagaaggca gaggttgtgg aaataatgct tgctgaggat
     5641 gaaaaggaaa ccggaaatga agaaaataaa ccggcggaaa aaacaaaatc atcggcacaa
     5701 aataacagca gcctttcggt ggatatcgat atttccgaag agaaaaaatg ccttgacagc
     5761 gggatcgaag caaatggtat tctggaagtc ctgacggaag gatatggttt tatcaggagc
     5821 gagaattatc tgccgggaga aaatgatgtt tatgtggcac cgagtcagat ccgtcgtttt
     5881 ggtttaaaga caggggatat tattgttggg aataccaggg taaaatcaca gaatgataaa
     5941 ttcggcgcac ttttatttgt gaagctgatc aatggtatga cgccgcagga agcgtccaag
     6001 agaaaaaatt ttgaggatct ggttccgatc tttccggatg aaagactgaa gctcgaaaca
     6061 cagggcggca gtattgccat gcggatcatg gacctgatgt ctccagtcgg caagggacag
     6121 cgcgggatga tcgtatctcc gccgaaagcc ggaaaaacaa cgttgttaaa ggatgttgcc
     6181 aagtctatta aaaggaataa tccggaaatg cacctgatca ttcttctgat cgacgaacgt
     6241 ccggaggaag tgacggatat caaggaggcg atcgaaggcc ctaatgtgga agtgatctat
     6301 tctacgtttg atgagctgcc ggagcatcat aaaagagttt ccgaaatggt gatcgagaga
     6361 gcaaagcgtc ttgtagagca cggcagagac gttattattc tgcttgacag catcacgcgt
     6421 ctgacaagag cctataatct gacggtgccg ccaagcggac gtacactttc aggcggtctt
     6481 gatccggcgg cgcttcatat gccgaaacgt ttttttggtg cggcaagaaa tatgcgcggc
     6541 ggcggttccc tgacaatact tgccacggca ttgatcgaga caggaagcaa aatggatgat
     6601 gtcgtatatg aagaattcaa gggtaccggt aacatggaaa tggtacttga cagaaagctt
     6661 tccgaaagaa gagtatttcc tgcgatcgat attccaagat ccggtacaag acgggaagac
     6721 ctgctgcttg acagggaaga gcttgaagcg atcaatgtga tgcggaaagc gctgaacgga
     6781 ctgaagcccg aggaagcggt cgaaaagatt ctggatatgt ttgtccgcac ccgcaataac
     6841 agggaatttg tggatatggt caaaaaaatc cgttttatat aaaaaatgct tgcactggta
     6901 atcaagctat ggtacaataa ctaagctgtt tcatgattga ttgttaatta ggaacgaatg
     6961 aatatagaga ggtgaaagaa atgaaagaag gaatccatcc aaaatattat caggcgaccg
     7021 tgacatgtaa ctgcgggaat acgtttgtaa cgggttctac gaaggaagat atccacgttg
     7081 agatctgttc aaagtgtcat tctttttata ccgggcagca gaaatcggta agagccgacg
     7141 gacgtataga taagttcaac aggaaatatg gtgttgaaag caaatagtga gattagaaaa
     7201 ggttgaggtt tgaaatctca acctcttttt catttcagag gatcattata tggcaaaaag
     7261 aaaaaaaaga gcaaagaagg accactattc cggtatcgga ggtcaggctg tgatcgaagg
     7321 tatcatgatg aaaaataagg ataagtattc ggttgcggtc aggacgcaga gcaatgagat
     7381 cgaagtcgtc ctggaagaat acgaatcgtt tgcaccgtct gagcgtctga cacagattcc
     7441 atttatcagg ggtgttttta attttatcga ttccatgatt ttaggcatca gaacgctgaa
     7501 ttattccgcc tctttttatg aagaagtgga cgagaaagac aaaaagaagc aaaagcctgc
     7561 ggacaagatg cttggggata attccgagaa gatcatgaca ggtctgaccg tgtttctttc
     7621 gatcgttatt gcagtcggtc tgtttatgct gcttccgtat tttctgtcgc agcttttgag
//
LOCUS       DAOSYE010000005         7397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6489_length_7397_cov_3.607192, whole genome shotgun sequence.
ACCESSION   DAOSYE010000005 DAOSYE010000000
VERSION     DAOSYE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6489_length_7397_cov_3.607192"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1239
                     /locus_tag="PLU43_00150"
     CDS             109..1239
                     /locus_tag="PLU43_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO26845.1"
                     /translation="MGKKIYLASPTMHGQEIEYIREAFDTNWIAPLGPNVNEFEKEFA
                     AYLGDGCYAAALSAGTAAIHLALIICGVKRDDIVFGSSLTFAATVNPITYIGAKPVFI
                     DSETDTWNMDPKALEKAFSLYPSVKAVISANLYGTPAKLDEISCICKKHGVPFIEDAA
                     ESLGAAVNGKQTGLFGDIGIFSFNGNKIITTSGGGMLVSRDESIVRRARFLATQAREP
                     ERHYEHKEIGFNYRMSNIVAGIGRGQLPFLEEHKRKKKELYEKYREAFSDIAQIHMNP
                     YEENTEPNFWLSCITIDEACDVKPLDIMLALEKEAIESRPIWKPMNLQPVFAANGFVQ
                     TVEGKSIGERVFDSGVCLPSDIKNTDEDMERIISIIRGLFGK"
     gene            1236..1844
                     /locus_tag="PLU43_00155"
     CDS             1236..1844
                     /locus_tag="PLU43_00155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO26846.1"
                     /translation="MIYKKYLKRVLDIVLSFLAIIILSPVYLVLAVLVRVKLGSPVLF
                     CQDRPGRYGKIFSMYKFRSMTDKRGADKELLSDEERLTRFGKKLRATSLDELPELFCI
                     LKGDMSLVGPRPLLVRYLPLYNERQRHRHDVRPGLTGLAQVNGRNAISWTKKFEYDVE
                     YTETLSFLLDMKILFKTVANVVKKEGISSENAATMEFFTGNE"
     gene            1881..2879
                     /locus_tag="PLU43_00160"
     CDS             1881..2879
                     /locus_tag="PLU43_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO26847.1"
                     /translation="MNTIHVLILSAGRRVELVNCFRSAAKELGIKSNILAADCSKTAP
                     ALYFADKHVIVPRIDSGNYIEAIIAVCKEEKVALIVPTIDTELLLLSEHKELIESSTG
                     AKLLVSDPAVIGICRNKAATQTFMEENSFLMPRLFTEEELAEGKLPFPLFIKPIDGSS
                     SINAFKVHNQEELDIYRKLIEKPLVQEYIEGEEYTVDVFLDFDATLITVVPRLRIATR
                     SGEILKGRIVRDREIIADVTRLMKLLKPIGHITVQCKKTDRGIAYIEINPRFGGGAPM
                     SIKAGADSCKNLYRLLNNEKLVYNEDYRENITFLRFDSCIMLNGNMEPENEKSRNF"
     gene            2857..3540
                     /locus_tag="PLU43_00165"
     CDS             2857..3540
                     /locus_tag="PLU43_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOO26848.1"
                     /translation="MKKAVIFDLDDTLAPEEEYVKSGYRCVAGHIEGKFLRGITAEKL
                     YEELYSLYETDHKNVFNRLLEAHSVFYTKENIRELVDVYRTHRPVLSFYEDVRPVLTA
                     LRERKIGTGIISDGYAMTQTQKLEVLGAKQLFDKIILTDELGREYWKPAYPVYEMMRL
                     FFQIDFSEMIYVGDNPQKDFYIGKEYPILTVRIDRGDGVYAHCDYKDGIREKVRIRSL
                     MELLPLTER"
     gene            3559..4782
                     /locus_tag="PLU43_00170"
     CDS             3559..4782
                     /locus_tag="PLU43_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO26849.1"
                     /translation="MEILYLTLSDPQLQKPGIYADLVNGLTERGHHVTIAFAAGPADT
                     EKTVLVTECGVRIVKVLVGELFRVNFIKKGINTLKIEPYLKRAVRKYFPKEQFDLVLY
                     ATPPVTFANVVKYCKSTYGCRAFLMLKDIFPQNAVDIGLFSENGLLHRFFQKKERELY
                     KVSDVIGCMSPGNMEYIRKHNPETEAKKLILFPNTVKVTPLLKDGQDIKARKDTVSFV
                     FGGNLGKPQAVDFLIRVINSKRLRACRAAKFLFVGNGSERDKVKAAFANSPNAEFIDF
                     MPPAEYRELMRRCDVGMISLDARFTIPNYPSRALSYMAEAKPILACTDRNTDIRELLT
                     EEADCGLWCASDDEEAFCENVERLCRDEGLREQLGNNGRRYLEEHFDVSRSVGLIEQY
                     GCHNKKETMEGRDDV"
     gene            4775..5803
                     /locus_tag="PLU43_00175"
     CDS             4775..5803
                     /locus_tag="PLU43_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOO26850.1"
                     /translation="MFEGKTLMITGGTGSFGNAVLNRFLDTEIREIRIFSRDEKKQDD
                     MRHQYHNTKIKYYIGDVRDPQSVKDAMNGVDYIFHAAALKQVPSCEFFPMEAVRTNVI
                     GTDHVLSAAIEAGVKKVICLSTDKAAYPVNAMGTSKAMMEKVFVAKSRTVDPEKTLIC
                     GTRYGNVMCSRGSVIPLFIEQIKAGKPLTVTEPAMTRFIMSLEEAVELVLFAFENAAA
                     GDIMVQKAPACTIEVLAQAVKELFHADNEVKIIGIRHGEKMYETLLTNEECSRAIDMG
                     AFYRVPADQRDLNYDKYFKEGDQKRTKLTEFNSNNTQILTVEQVKEKLLKLAYIRDEI
                     KAWDAEDI"
     gene            5805..6926
                     /locus_tag="PLU43_00180"
     CDS             5805..6926
                     /locus_tag="PLU43_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOO26851.1"
                     /translation="MNILVTGANGFIGKNLILELENAGYSSVYPYDVDTKEELLDDYA
                     RCCDFVFHLAGVNRPKDEKEFMEGNFGFTSVLLEKLKKYHNPAPILITSSIQAQLDNP
                     YGKSKRAGEELLLSYAEETGARVIIYRLPNVFGKWCRPNYNSVVATFCHNIANGLDIR
                     LDDPKTVMNLVYIDDVIREFFAAMDGKEHRNGDFSSVPAVHTVRLGEIAESLYRFRNG
                     RSDLSVPNLSNEFEKKLYSTYLSYLPKDDFSYPLHMHTDERGSFTEFLRTPDRGQVSV
                     NISRPGVTKGNHWHHTKCEKFLVVKGTGCIRFRKIGEEEIITYHVSDKNLTVVDIPTG
                     YTHSITNEGDTDMITVMWANEAFDPDRPDTYFEKVEVKP"
     gene            6926..>7397
                     /locus_tag="PLU43_00185"
     CDS             6926..>7397
                     /locus_tag="PLU43_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOO26852.1"
                     /translation="MEKLKVMTIVGTRPEIIRLSAVIKCADKYFDHILVHTGQNYDYT
                     LNQIFFEDLKLREPDHYLESVGADLGETMGNIIAGSYRLMQAEKPDAVLILGDTNSAL
                     SAISAKRLKIPIFHMEAGNRCWDWNVAEMINRKIVDHISDINLPYTEHARRYLLS"
BASE COUNT         2137 a         1417 c         2019 g         1824 t
ORIGIN      
        1 cgaagagctg aaagcagaat ctaaagaaga agtcgataat gatgcgatca gggaatgggt
       61 cagaaagctt gtaccgactt accaaataaa aaaggctgga gaatgagcat gggaaaaaag
      121 atttatctgg catctccgac gatgcacgga caggaaatag agtatatcag agaagcattc
      181 gatacaaact ggatcgcacc gctcggaccg aatgtcaatg aatttgaaaa ggaatttgcg
      241 gcgtatctgg gagacggctg ttatgcggcg gcactcagtg ccggtacggc agcgatccat
      301 ctggcactga tcatctgcgg tgtgaaacgg gatgatatcg tgtttggttc ttccctgaca
      361 tttgccgcca cagtgaaccc gatcacttat atcggcgcaa agccggtgtt tatagacagt
      421 gaaacagata catggaatat ggaccccaag gcgctggaaa aggcattttc tctgtatccg
      481 agcgtaaagg cggtcatttc tgccaatcta tacggaacgc ccgcaaagct ggatgagatt
      541 tcctgtatct gcaaaaagca tggggtgccg tttatagagg acgcagccga gtctctcgga
      601 gctgcggtaa acggaaagca gaccggtctt ttcggagata tcggtatttt ttcgtttaat
      661 gggaacaaga tcattacgac ttccggcggc ggcatgctgg tcagcaggga tgaatcgatc
      721 gtcaggagag cccgttttct tgcaacacag gcaagggagc cggaacgtca ttatgagcac
      781 aaagagatcg gttttaatta cagaatgtca aatatcgtgg ccgggatcgg acgcggacag
      841 ctgccgtttc ttgaagaaca taaaagaaag aaaaaagagc tttacgagaa atacagggag
      901 gctttttcag atatcgcgca gatccatatg aatccttacg aagaaaatac ggagccgaat
      961 ttctggcttt cctgcattac gatcgacgaa gcctgtgatg taaagcccct tgatatcatg
     1021 cttgcacttg aaaaggaagc gatcgaatca aggccgatct ggaagccgat gaacctgcag
     1081 cctgtttttg cagccaacgg atttgtgcag acagtggaag gaaaaagtat cggtgagcgt
     1141 gtgtttgaca gcggggtctg cctgccttcc gatatcaaaa atacagatga ggatatggag
     1201 cgtattattt caattatcag gggattattt ggaaaatgat ttataaaaaa tatttgaaac
     1261 gcgtattgga tattgtactt tcttttctgg caattatcat tttatcgccg gtctatttgg
     1321 ttttggcagt actggtccgc gtaaagctgg gcagtccggt gctgttttgt caggacaggc
     1381 cgggcagata cggtaaaata ttttccatgt acaaatttcg ctccatgacc gataaaagag
     1441 gtgcggacaa agaactgctt tccgatgaag aacggctgac gcggtttggg aaaaaactgc
     1501 gtgcaacaag tctggatgag ctgcctgagc tgttctgcat cctgaaagga gatatgtcgt
     1561 tagtcggtcc ccgcccgctg cttgtaaggt atctgccgct ctataacgaa cgtcagcgtc
     1621 acaggcatga tgtcagaccg ggtcttaccg gacttgcaca ggtgaacgga agaaatgcga
     1681 tcagctggac aaagaaattt gagtatgatg tggaatatac ggaaacgctt tcgtttctcc
     1741 tcgatatgaa aattttgttt aagacggtcg ccaatgtcgt aaaaaaggaa gggatcagtt
     1801 ccgagaatgc ggcgacaatg gagtttttta ccggcaatga ataggaacct gcaggcagaa
     1861 ggataaacag gaggaagcgc atgaatacga ttcatgtttt gattttaagt gcgggacgga
     1921 gagtggaact ggtaaactgt tttcgctccg ccgcgaaaga gctcggcata aagagcaata
     1981 ttctggctgc ggactgcagc aagactgccc ccgcccttta ttttgcggat aagcacgtca
     2041 tcgttccaag aattgattcc ggtaattata tagaggcgat cattgctgtt tgcaaggaag
     2101 aaaaggtggc actgatcgtt ccgacgatcg acacggaact gctgctgctt tcggagcata
     2161 aggagctgat cgagagcagt accggagcaa agcttttagt ttcggaccct gctgttatcg
     2221 gaatatgcag gaataaggca gcaacgcaga cttttatgga agaaaacagc tttctgatgc
     2281 cgcgtttgtt tacggaagaa gagcttgcgg aagggaaact gccgtttcct ctttttatta
     2341 aaccgatcga cggcagttcc agcatcaatg cgttcaaggt gcataatcag gaggagcttg
     2401 acatatacag aaagctgatc gaaaaaccgc tcgtacagga atatatcgaa ggggaagaat
     2461 atacggtcga tgtgtttttg gactttgatg ctacgcttat tacggtcgta ccgcggcttc
     2521 ggatcgcgac acgaagcgga gaaatactaa agggccggat cgttcgtgac agggagatca
     2581 ttgcggatgt taccaggctg atgaaactgc tgaagccgat cggccatatt acggtgcagt
     2641 gtaaaaaaac agacagggga attgcctata ttgaaatcaa tccgcgtttt ggcggcggtg
     2701 caccgatgtc gatcaaggcc ggcgccgatt cctgcaagaa cctttacaga ctgcttaata
     2761 atgagaaact tgtttataat gaggattaca gggaaaatat tacgtttttg cggtttgaca
     2821 gctgtataat gttaaacggg aacatggagc cggaaaatga aaaaagccgt aatttttgat
     2881 ttggatgata cgcttgctcc tgaagaggag tatgtaaaga gcggttaccg atgcgtggcc
     2941 ggtcatatcg aaggtaaatt tctgcgcggg atcactgcgg agaaacttta tgaagagtta
     3001 tattcgctgt atgaaacgga tcataaaaat gtattcaacc gccttcttga agcgcacagc
     3061 gtattttata caaaggaaaa catacgggag cttgtagacg tctatcggac acacaggccg
     3121 gtgctgtctt tttatgagga tgtgcgcccg gtattgactg cactccggga aagaaagatc
     3181 ggaacaggca ttatttcaga cggctatgca atgacacaga cccaaaagct ggaggtgctt
     3241 ggtgcaaagc agctgttcga caagatcata ctgaccgatg aactggggag ggaatactgg
     3301 aaacctgctt atccggtcta tgaaatgatg agattgtttt tccagatcga tttttcagaa
     3361 atgatctatg tcggtgataa tccgcaaaag gatttttaca tcggaaaaga gtatccgatc
     3421 ctgactgtca ggatcgatcg cggggacggt gtttatgcgc attgtgatta taaagacggt
     3481 atcagggaaa aggtgaggat ccgctcgctt atggaattgc tgccgcttac ggagagataa
     3541 tggaacggaa gggaatttat ggagatactg tatttgacat tgagtgatcc gcagcttcaa
     3601 aaacccggta tttatgcgga tttagtgaac ggactgaccg agcgcggaca tcatgtgacg
     3661 atcgcatttg ccgcaggtcc cgccgatact gaaaagacgg ttcttgttac cgaatgcggc
     3721 gtcaggatcg taaaggttct ggtcggggaa ctctttcgtg taaattttat taaaaaaggg
     3781 atcaatacgt taaaaataga gccctatctg aaaagggctg tcagaaagta ttttccaaag
     3841 gaacagtttg atcttgtgct ttacgcaacg ccgcctgtga cgttcgcaaa tgtcgtaaaa
     3901 tattgcaaaa gcacttacgg atgcagggca tttctgatgc tgaaggatat ttttccgcag
     3961 aatgcggtcg atatcggact tttttcggaa aatgggctgc tgcatagatt ttttcagaaa
     4021 aaagaacggg agctgtacaa agtatccgat gtgatcggct gcatgtcacc gggcaatatg
     4081 gagtatatca ggaaacacaa tccggaaacg gaagcaaaaa agctgatcct ttttccgaat
     4141 acggtgaaag tgacgccgct tttaaaagac ggccaggata taaaggcgcg aaaggatacg
     4201 gtaagtttcg tattcggagg gaatcttgga aaacctcagg cagttgattt tctgatccgg
     4261 gtcattaatt caaaacggct tagagcctgt cgggcagcga aatttctgtt tgtcggaaac
     4321 ggcagtgagc gggacaaggt aaaagccgct tttgcaaata gtccgaatgc ggaatttatc
     4381 gattttatgc cgcctgcaga atatcgggaa ttgatgaggc gctgtgatgt cggtatgatt
     4441 tccctggatg cgcggtttac gataccaaac tatccgtcaa gggcgcttag ttatatggcg
     4501 gaagccaagc cgatccttgc ctgtacggac agaaacacgg atatcaggga gcttctgaca
     4561 gaagaggcgg actgcggact gtggtgtgct tcggatgacg aggaagcctt ttgtgaaaat
     4621 gtggagcggc tttgcaggga cgaagggctc cgggaacagc ttggaaataa cggaaggcgt
     4681 tatctggaag agcattttga cgtgagccgt tcggtcggac tgatcgagca gtatggctgc
     4741 cataataaga aagaaacgat ggaaggaaga gacgatgttt gaaggaaaga cattaatgat
     4801 cacgggaggg accggttcgt tcggcaatgc ggtattaaat cggtttttgg atacggagat
     4861 cagagagatc cgtattttca gcagggatga aaaaaaacag gatgatatga ggcatcagta
     4921 tcacaataca aagatcaaat attatatcgg tgatgtcaga gatccgcaga gtgtaaagga
     4981 cgccatgaac ggtgttgatt atatttttca tgcggcggcg ctcaagcagg tgccgagctg
     5041 tgaatttttc ccgatggaag cggtacggac aaatgtgatc ggcacggacc atgtactgag
     5101 tgcggcgatc gaagccggtg taaaaaaggt catctgcctt tccacggata aggccgctta
     5161 tcccgtgaat gcaatgggaa cgagcaaggc gatgatggaa aaggtattcg ttgcaaagtc
     5221 aagaacggtc gatccggaaa aaacactgat ctgcgggaca agatacggaa acgtcatgtg
     5281 ttcgagaggt tctgtcattc cgctgtttat cgagcagatc aaggccggaa aaccattgac
     5341 ggtaacggag cctgcgatga caagatttat catgagtctg gaggaagcgg tggagcttgt
     5401 gctctttgct tttgaaaatg ccgcggccgg agatattatg gtacagaaag caccagcctg
     5461 tacgatcgaa gtactggcgc aggcagtgaa agaattattc catgcggaca atgaggtcaa
     5521 gatcatcggg atccgtcacg gagaaaaaat gtatgaaaca cttcttacga atgaggaatg
     5581 ttcccgtgcg atcgatatgg gcgcgtttta cagggtgccg gccgaccaga gagatctgaa
     5641 ttatgataaa tatttcaaag aaggcgatca gaaacggaca aagcttactg agttcaattc
     5701 caataatacg cagatcctta ctgtggaaca ggtcaaggaa aaactgctga agctggctta
     5761 tatcagggat gaaataaagg catgggatgc agaggatatt tgatatgaat attttagtga
     5821 cgggtgcaaa tggatttatt ggaaaaaatc tgatacttga attggaaaat gcgggttatt
     5881 catctgtata tccatatgat gtcgatacaa aggaagaact gctggacgat tatgcaaggt
     5941 gctgtgattt tgtattccat ctggcaggtg ttaaccggcc gaaagacgaa aaagaattta
     6001 tggaaggcaa tttcgggttt acttctgtgc tgcttgaaaa attaaagaag tatcataatc
     6061 ccgcgccgat actcattacg tcatcgatcc aggcgcagct tgacaatccg tacggaaaga
     6121 gcaagcgtgc aggggaagag cttttgcttt cctatgcgga agaaacagga gcgcgggtga
     6181 tcatttacag gctgccgaat gtattcggaa aatggtgccg tccgaattat aacagcgttg
     6241 tggcgacatt ctgtcataat atcgcaaatg gtctggacat cagactggat gatccgaaga
     6301 cagtcatgaa cctggtttat atcgatgatg tcatccggga gttttttgcg gccatggatg
     6361 gaaaggagca caggaacggg gatttttcgt cggtgccggc cgtgcatacg gtcaggctcg
     6421 gggagatcgc tgagtcgtta taccggttcc ggaatggcag aagtgatctg tcggtgccga
     6481 atctgtcaaa tgaatttgaa aaaaagctgt acagtaccta tcttagttat ctgcctaaag
     6541 atgacttttc ctatccgctg catatgcaca cggatgagag gggatcattc accgaatttc
     6601 ttcgtacgcc ggatcgggga caggtgtctg ttaatatcag cagaccgggt gtgacgaaag
     6661 gaaatcactg gcatcatacg aaatgcgaga aatttcttgt cgtaaaagga accggatgca
     6721 tcagattccg caaaatagga gaagaggaga tcataaccta tcatgtttcc gacaaaaatc
     6781 tgacggtggt ggatatcccg acaggatata cacacagtat taccaatgaa ggcgatacgg
     6841 atatgataac ggtcatgtgg gcaaatgaag catttgatcc ggacagaccg gatacttatt
     6901 ttgaaaaggt ggaggtaaag ccgtaatgga gaaactgaag gtaatgacaa ttgtcggaac
     6961 aaggccggag atcattcgtc tgtctgccgt tatcaaatgt gcggataaat atttcgatca
     7021 cattctggta catacgggac agaattatga ttatacgctg aatcagattt tttttgagga
     7081 tcttaaactg agagagcctg atcattatct ggaaagcgtc ggtgcggatc ttggtgagac
     7141 aatgggcaat attatcgcgg gctcttatcg tctgatgcag gcggaaaagc cggatgccgt
     7201 actgatactc ggcgatacga actccgcact ttctgcgatt tccgcaaaac gtctgaaaat
     7261 accgattttt catatggagg caggcaaccg ctgctgggat tggaatgtag cggaaatgat
     7321 caaccggaag atcgtcgatc atatttccga tatcaacctg ccgtatacgg agcatgcaag
     7381 acgctatctg ctttcag
//
LOCUS       DAOSYE010000006         7397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6490_length_7397_cov_3.436121, whole genome shotgun sequence.
ACCESSION   DAOSYE010000006 DAOSYE010000000
VERSION     DAOSYE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6490_length_7397_cov_3.436121"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLU43_00190"
     CDS             <1..294
                     /locus_tag="PLU43_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO26853.1"
                     /translation="VMPTEGALLCSQVFLLCVMPTAIMQFNGLIKGRTARITTNTVCV
                     IFAAVAVVGRLLSGVHWFTDILGGVLLSSALVMLYYSVNILIESKKTQGNHIL"
     gene            complement(328..2679)
                     /locus_tag="PLU43_00195"
     CDS             complement(328..2679)
                     /locus_tag="PLU43_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO26854.1"
                     /translation="MKKKNLSGKTGVKIIWVLISYIGILSLSFGLTVYATVYERTGYG
                     TLTDIFSSEDQEYLDSDYFNGVYLDTVDSLADYIRYQKVFETENVYDGNKLIDIDTYV
                     NNVINRNGVPDAVEGNKNGESPCYYLDDLLDWSESGYEFLSDTEETEADAGSGLLLNE
                     QFLPVNAGSLYDFIETEEEKEQLEETLTTVLAHISEEFIYYKELESRFSDTNFKYYVY
                     DEEKDNYYANTELVNTQTEAASELKKYGRFVETDSKEKGYETNLNIGYRELYDRFCSF
                     DKLFEGDYQIVTVVDTSFPIEDVFTQQDMPYDKLLPWLKILFIVMIFGAAAVIVSLIA
                     MTGCAGAVSDSEETRLNWFDKIKTEFAIVIMLFLGGISLFAGVRAFPYNADDSPFLQV
                     MIYTALFTALFMCLEHTWFLIGYTSLVRRIKAHTMWSNSLCCWIITGVSSVLSAIKVT
                     GRLILFYTGFVLFQIIFVYFCIRAGMLFWMLILIAVDLLAGIRILEEAVGRQKIKDGL
                     RKIADGNLEYKIPLENLGNENRDMAEAVNRVGEGLHNAVEESLKNERLKTDLITNVSH
                     DIKTPLTSIINYVDLLKRENIGNEKIKGYISVLDSKSQRLKQLTEDLVEVSKVSSGNI
                     NLKMERLNFVELIHQTIGEFNEKFQIKNLELITNLPKESVFIIADGRRLWRVIENLYN
                     NIAKYALENTRVYADLTAAEGVAQFSLKNISAQALNFSGDELTERFIRGDVSRSTEGS
                     GLGLSIAKNLTESQGGEFHIYLDGDLFKVTVRFAVAKMQEGEL"
     gene            complement(2720..3418)
                     /locus_tag="PLU43_00200"
     CDS             complement(2720..3418)
                     /locus_tag="PLU43_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO26855.1"
                     /translation="MEQMNILVCDDDREIVEAIQIYLENEGYHIFCAYDGEQAIRLLE
                     KEEFHLLIIDVMMPKLDGIRATLKIREISSIPIIILSAKTEDTDKILGLNVGADDYLA
                     KPFNPLELVARVKSQLRRYTKLGAIAGDNKSIFSVGGLMINDDLKEVRVEDELVKLTP
                     IEYNILLLLVKNIGKVFSINQIYENIWNEDAIGADNTVAVHIRHIREKIEINPKEPRY
                     LKVVWGVGYKIEKQ"
     gene            complement(3521..4006)
                     /locus_tag="PLU43_00205"
     CDS             complement(3521..4006)
                     /locus_tag="PLU43_00205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO26856.1"
                     /translation="MKNTEIEIRPLEYKEWADAMQLAWDTFLVFEAPEYSPEGVRNFH
                     DFVRDPMLKKMFLRGKYQTLGAFFNGTIIGILGVRSKNHISLLFVDSGYHHQGIATAL
                     LKKMFYYVRNELSIHEMTVNSSPYAIDFYHKIGFFDLGNEITTDGIRYTPMQIDLKDE
                     S"
     gene            complement(4024..5025)
                     /locus_tag="PLU43_00210"
     CDS             complement(4024..5025)
                     /locus_tag="PLU43_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HOO26857.1"
                     /translation="MKRAICLLLVLTMVFGMTTAVFAEQTIVFDEEGNGTVSGNSEET
                     ASVTTDKAYLALGSDLTQDQLTTVLGLMGISSAELGNYNVVYVTNEDEHNYLDQYISS
                     DVIGTKSLSSVLVRPAEEGYGVVVTTKNINYCTTNMYRNALITAGVENADIMVVAPVQ
                     ISGTAGLIGALKAYEQMSGTTVTDEALDTALDELVSTGELSETVGSSSEAEDLISYIK
                     AQIAANDLDTEEEIEAAIRQGMKDKNISLTDEEVQEIVDLMMKIKSMGIDFDVLAEQA
                     DDIYAKYKTQIDNGTFQISDVDINDLGIGKIISNAASNFIENVGNSVKSFFSKLFSK"
     gene            5268..5624
                     /locus_tag="PLU43_00215"
     CDS             5268..5624
                     /locus_tag="PLU43_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO26858.1"
                     /translation="MNNLKLLRESRGLSQQKLAEKFNLSQQSVYKYENQLAEPDLTTL
                     MQFAEFFHTSVDYLIGYAKDDKPYSDASLMENLLTPNEAHHLVMYRHLSADNRKHLDS
                     ILESFAPDDNYKTKFQ"
     gene            complement(5636..>7397)
                     /gene="metG"
                     /locus_tag="PLU43_00220"
     CDS             complement(5636..>7397)
                     /gene="metG"
                     /locus_tag="PLU43_00220"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOO26859.1"
                     /translation="VKGLWDLMNTSYDKFIRTTDADHELQIQKIFKKLYEQGDIYKGS
                     YEGLYCTPCESFWTESQLVDGKCPDCGREVAPAKEGAYFLKLSKYADRLIEHINQHPE
                     FIQPVSRKNEMMNNFLLPGLQDLCVSRTSFQWGIPVDFDNKHVVYVWLDALSNYITGI
                     GYDAKGNSTQQYKKLWPADLHLIGKDIVRFHTIYWPIILMALGEPLPKQVFGHPWLLQ
                     GGEKMSKSKGNVIYADDLVKLFGVDAVRYFVLHEMPFENDGVITWELVVERFNSDLAN
                     ILGNLVNRTISMSNKYFDGIVKATGETEAVDADLKTVVTGTRDKVAEKMGTLRVADAL
                     TEVFHLFRRCNKYIDETEPWVLAKDVNDVSKKARLSEVLYNLVESICIGANLLKPFMP
                     ETTDKILSQMNDKERTYETLSEYGLYNSGTKVIEKPEILFARLDVKEIMPKVEEIRAA
                     QKAEYEAENSAAASDAEPEEAVIEIEKKPEITYEDFGRLQFQVGRIIACEEVPKSKKL
                     LCSKVQIGKEVKQIVSGIKQYYSAKEMVGKNVMVLVNLKPATLAGVLSEGMLLCAEDG
                     EGKLALVVPEKPMPAGAEIC"
BASE COUNT         1975 a         1855 c         1247 g         2320 t
ORIGIN      
        1 gtcatgccca cagaaggagc attgttgtgc agtcaagtat tccttctatg tgtcatgccc
       61 acggcgatca tgcagttcaa cggtctgatc aaggggcgaa ctgcaagaat tacgaccaac
      121 accgtctgtg tcatatttgc tgccgtcgct gtagtcggac ggttgctgtc aggtgttcac
      181 tggtttaccg atattcttgg cggtgtgcta ttgagttcgg cgcttgtcat gctgtattat
      241 tcggttaata tattgattga atccaaaaaa actcagggca atcatatcct ttgatataat
      301 tgccctgggt ttatttcctg tttactatta cagttcccct tcctgcatct tcgcaacagc
      361 gaaccggacc gttaccttaa acagatctcc gtccagataa atatgaaact ctccgccctg
      421 ggattctgtc agattttttg cgatcgataa cccaagaccg ctcccttcgg tacttctgga
      481 aacgtctcct ctgataaagc gttctgtcag ttcgtctcct gaaaaattaa gcgcctgtgc
      541 cgatatattt tttaaagaaa actgtgctac accttctgct gctgtcagat ccgcataaac
      601 tcttgtattt tccagtgcat attttgcgat gttgttatat aggttttcaa tgactctcca
      661 tagtctccgc ccgtcggcaa taataaacac cgactctttc ggcagatttg taatcagttc
      721 cagatttttt atctgaaatt tctcattgaa ttccccgatc gtctgatgga tcagttccac
      781 aaaattcagc cgctccatct ttaaattgat atttccggag ctcacttttg aaacttctac
      841 cagatcctcc gtcagctgtt tcagtctctg tgatttgctg tccagcacgc tgatatagcc
      901 ttttatcttt tcattgccga tattttcccg tttcagcaga tccacataat tgataatgga
      961 agtcagcggt gttttgatat catgggaaac attcgtaatc aaatccgttt tcagccgttc
     1021 atttttcagg ctttcctcca ctgcgttatg aagtccttcc cccacgcggt tcactgcttc
     1081 cgccatgtcc ctgttctcat tgcccaaatt ctcaagcggg atcttatatt ccagatttcc
     1141 atctgcaatt ttacggagtc cgtcttttat tttctgtctg cctaccgctt cttctaaaat
     1201 cctgatacct gccaggagat caactgcgat cagtatcagc atccagaaaa gcatgccggc
     1261 cctgatacaa aaatatacga aaatgatctg aaacaataca aaacccgtat aaaagagaat
     1321 aaggcggccg gtaactttta tggcggacaa taccgaacta actcctgtaa tgatccagca
     1381 gcaaagacta ttgctccaca tcgtatgtgc cttgatcctt ctgacaagac ttgtatatcc
     1441 tatcagaaac catgtatgct ccagacacat aaacagggct gtaaaaagag ccgtatagat
     1501 catcacctgc aaaaaaggag aatcatccgc attatacggg aaagcgcgta cgccagcaaa
     1561 cagggaaatc cctcccaaaa acagcatgat cacgattgca aactctgttt ttattttatc
     1621 aaaccaattc agtctggtct cttcagaatc cgaaacagct ccggcacagc ctgtcattgc
     1681 aattaatgaa acaatgaccg ccgccgctcc gaatatcatt acgataaaca agattttaag
     1741 ccatggaagc agcttgtcat acggcatatc ctgctgcgta aaaacatcct ctattggaaa
     1801 gcttgtatca acaacagtca cgatctgata gtccccttca aacagtttat caaagctgca
     1861 gaatctgtca taaagctcac gatatccaat attcagattt gtttcatatc ctttttcctt
     1921 tgaatccgtt tctacaaatc tgccgtattt ttttaattcg gaagcagctt ccgtctgtgt
     1981 gtttacaagc tccgtatttg cataatagtt atctttctct tcatcataaa cataatattt
     2041 gaaatttgta tccgaaaatc tgctttccag ttctttataa taaataaact cctcagatat
     2101 atgtgccagt acggtcgtaa gtgtctcttc cagctgctct ttttcttcct ctgtctctat
     2161 aaaatcataa agactgccgg cgtttaccgg taagaactgt tcatttaaga gcagtccgct
     2221 gccggcatct gcctccgttt cttctgtatc ggaaagaaat tcatatccgc tttccgacca
     2281 gtccagcaga tcgtccagat aataacacgg actttctccg tttttgttcc cttcaacagc
     2341 atccggtacg ccgtttctat ttatgacatt atttacatat gtgtcaatat cgatcagttt
     2401 atttccatca tatacatttt ctgtttcaaa tactttctga tagcggatat aatcagcaag
     2461 actgtcgacc gtatcaagat aaacaccatt gaaatagtcg gaatccagat attcctgatc
     2521 ttcagaacta aaaatatccg tcagcgtgcc ataaccggtt cgttcataaa cggttgcata
     2581 tacggtaagt ccgaatgaca ggcttaaaat acctatataa gaaataagca cccatattat
     2641 tttcacccct gtctttccgc tcaaattttt tttcttcatt attgattcct ctttctttta
     2701 ccgcatgcag gcctgctgcc tattgctttt cgattttata tccgactccc catactactt
     2761 tcaaatacct tggttctttt ggattgatct ctattttttc cctgatatgc ctgatatgca
     2821 cggcaaccgt attatcggcg ccgatcgcgt cttcattcca gatgttttca taaatctgat
     2881 tgatagaaaa aacttttccg atatttttta caagcaagag caaaatatta tactcaatcg
     2941 gcgtcagctt tacaagctca tcctcaacac ggacctcttt caaatcgtcg ttgatcataa
     3001 gtcctcccac actaaatatg gacttgttat cgcccgcgat cgcaccaagc tttgtataac
     3061 gcctaagctg tgattttact ctggccacaa gctccaatgg attgaacggc ttcgccagat
     3121 aatcgtctgc tccgacattc agtccgagaa tcttatccgt atcctccgtt ttagcagata
     3181 aaataatgat cgggatactg cttatttcac gaattttcag tgttgcacgg atcccgtcca
     3241 gtttcggcat catgacgtca ataatcaaaa ggtgaaactc ttctttttcc agcagccgga
     3301 tcgcctgttc tccgtcataa gcgcaaaaaa tgtgataccc ttcattttcc agatagatct
     3361 gaatcgcctc cacaatttcc ctgtcatcat cgcaaaccaa tatattcatc tgttccataa
     3421 ttcctccctg caccatggcc ggtaatcgcc tgacctcctt accatactca gtaaaacata
     3481 ttttttttaa aatcaaattc ggatttttat taagagttct ttaagattcg tcttttagat
     3541 cgatttgcat cggtgtatag cggattccgt ccgttgtgat ttcatttccc aagtcaaaaa
     3601 accctatttt atggtagaaa tcaatggcat atggtgagga attcactgtc atttcatgaa
     3661 tagaaagttc atttcttaca taataaaaca tcttttttaa aagagcggtc gcaattccct
     3721 gatgatgata tccgctgtcc acaaagagca gggaaatatg atttttgctg cgaacaccca
     3781 aaatgcctat gatcgtcccg ttaaaaaagg ctcccagcgt ctgatatttc cccctcagaa
     3841 acattttttt caacatcgga tcccttacaa aatcatgaaa atttctgaca ccttccggcg
     3901 aatattccgg cgcttcaaat accagaaacg tatcccatgc caactgcatt gcgtccgccc
     3961 attctttata ttcaagcggg cgaatttcaa tttctgtgtt tttcatttca tctctccgcg
     4021 attttatttt gaaaataatt tactgaagaa acttttgacc gaatttccga cattctcaat
     4081 aaaattactg gctgcattgg aaattatttt ccctatgcca agatcgttga tatcaacatc
     4141 actgatctga aaagttccgt tgtcgatctg tgttttatat tttgcataaa tatcgtccgc
     4201 ctgctcggca agcacatcaa aatcaattcc cattgatttg atcttcatca tcagatcaac
     4261 aatttcctgt acctcttcat ctgtcaggct gatattttta tctttcattc cctgtctaat
     4321 tgctgcttcg atttcttctt ccgtatccag atcatttgcc gcaatctgcg ctttaatata
     4381 agaaatcaaa tcctctgctt cgctgctgct tcctactgtc tcagataatt cccccgtaga
     4441 aacaagctca tccaatgcag tatccagtgc ttcgtccgtt accgtcgtac cggacatctg
     4501 ctcataagcc ttcagtgcac cgatcagtcc tgccgtaccc gaaatctgca cgggtgcgac
     4561 taccataatg tccgcatttt ccacacccgc cgtgatcaat gcattccggt acatattcgt
     4621 cgtacagtaa ttaatgtttt ttgtcgtaac aacgacacca tatccttctt ctgccgggcg
     4681 caccaatacc gatgacaatg atttcgtccc gatcacatcc gagctgatat actgatccag
     4741 ataattatgt tcatcttcat ttgtaacata aaccacatta taatttccaa gctcggccga
     4801 agaaattccc atcaggccaa gtacggtagt aagctgatcc tgcgtcagat cagaacccag
     4861 cgccaaatat gccttgtctg tcgttacaga agccgtttct tcagaattac cggaaaccgt
     4921 tccgtttcct tcctcatcaa aaacaattgt ttgttcagca aataccgctg ttgtcattcc
     4981 aaacaccatt gtcaaaacca ataacaggca aattgctctc ttcataaata gattcctcct
     5041 tattattcat acgtcccgcc gcatatttgc aagatacttc tttttattca aattgtcaat
     5101 actttctatt aaatattaaa actatcttca gaaaccggcc attttataaa acaacctgtg
     5161 gttgttttta aaaacaacaa tttgtcttta tataggacaa aattccgttg ttacaataaa
     5221 tagagaagat tctattttat ttttggaatt gtgcgggagg ttctgacatg aacaatttaa
     5281 aactattgcg ggaatcaagg ggcttaagcc agcaaaaact tgctgaaaaa ttcaacttga
     5341 gtcagcagtc cgtatataaa tatgaaaatc agcttgcgga accggatctt acaaccctga
     5401 tgcagttcgc ggaattcttt catacatccg ttgattatct gatcggatat gcaaaagatg
     5461 acaagcccta ttcggacgct tccctgatgg aaaatctgct gactccgaat gaggcccatc
     5521 accttgtcat gtatcgtcat ttatctgctg ataaccgaaa acatctggac agtattctgg
     5581 aaagttttgc tcctgacgac aattataaaa ccaaatttca ataatccata tgctttcagc
     5641 agatttccgc tccggcgggc atcggttttt ccggtactac aagcgcaagt tttccctccc
     5701 cgtcttcggc acataaaagc attccttccg acaatacgcc ggccagtgtc gccggtttta
     5761 aattgacaag caccatcaca tttttcccga ccatctcttt tgcactgtaa tactgcttaa
     5821 ttccggatac gatctgtttt acttcctttc cgatctgaac tttggagcag agcagttttt
     5881 ttgactttgg aacttcttca caggcaatga tcctgcctac ctgaaactgc agcctcccaa
     5941 aatcctcata tgtgatctcc ggtttctttt caatctcgat cactgcttct tccggctcag
     6001 catcactggc cgctgcgctg ttctccgctt catactctgc tttctgtgca gccctgatct
     6061 cttccacctt tggcatgatc tctttaacat ccagtctcgc aaataatatt tccggctttt
     6121 cgatcacttt cgttcctgaa ttgtacaatc cgtattcgga aagtgtctca tatgttcttt
     6181 ctttatcatt catctgcgac agaattttat ccgtagtctc cggcataaac ggttttaaca
     6241 aatttgcgcc gatacagata ctttcgacca ggttataaag aacttccgaa agacgggctt
     6301 tctttgacac atcatttaca tcttttgcaa gcacccacgg ctctgtttca tcgatatatt
     6361 tattgcagcg tctgaataaa tggaaaactt ccgtaagcgc atccgccacg cgcaaagtcc
     6421 ccatcttctc tgcaacttta tctcttgtgc cggtcacgac tgtcttcaga tcggcatcta
     6481 ccgcctcagt ctctccggtg gcttttacga tcccgtcaaa atatttgtta ctcatcgata
     6541 tcgtacggtt tacaagattc ccgagtatat tggccaaatc cgaattaaaa cgctcaacta
     6601 ccagctccca tgtaatgacc ccgtcatttt caaaaggcat ttcatgcagt acaaagtaac
     6661 ggacagcatc tacaccaaac agctttacaa gatcatccgc atagatcaca ttccctttgg
     6721 atttactcat tttttccccg ccctgcaaaa gccatggatg gccgaatacc tgtttgggaa
     6781 gcggttcccc aagtgccatc agaataatcg gccagtagat cgtatgaaaa cggacaatat
     6841 cttttccgat caaatgcaga tcggcaggcc aaagcttttt atactgctgt gttgaattgc
     6901 cttttgcatc ataaccgatc ccggtaatat agttcgaaag cgcatccagc catacataaa
     6961 ccacatgctt attgtcgaaa tcgaccggaa tcccccactg aaacgacgtc cttgagacac
     7021 acagatcctg cagacccggc agcagaaaat tattcatcat ttcatttttt ctggataccg
     7081 gctgaataaa ttccggatgc tggttgatat gctcgatcag tctgtccgca tatttgctga
     7141 gttttaaaaa atacgctccc tcttttgcgg gcgcaacttc ccgtccgcag tccgggcact
     7201 tcccgtccac aagctgcgat tcagtccaga acgactcgca cggcgtacaa tacagtcctt
     7261 cataagaacc tttataaata tctccctgtt cataaagttt tttaaatatc ttctgtatct
     7321 gcaattcatg atcggcatct gtcgtcctga taaatttgtc ataggacgta ttcattaaat
     7381 cccaaaggcc ctttacg
//
LOCUS       DAOSYE010000007         7367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6520_length_7367_cov_3.442834, whole genome shotgun sequence.
ACCESSION   DAOSYE010000007 DAOSYE010000000
VERSION     DAOSYE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6520_length_7367_cov_3.442834"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLU43_00225"
     CDS             <1..208
                     /locus_tag="PLU43_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102920.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOO26860.1"
                     /translation="VEDTCIGCGLCAEKCPVNAIKIQNGKPVWVREQCVMCLKCLHHC
                     PKFSIQYGRHTKKHGQYRNPNVRG"
     gene            251..1804
                     /locus_tag="PLU43_00230"
     CDS             251..1804
                     /locus_tag="PLU43_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOO26861.1"
                     /translation="MENNPIIYSDFPDPDVIRVDDTYYMISTTMHFMPGGVILRSYDL
                     VHWEIFTYVYDSLDDTPGQRLEGAAAVYGKGMWAATLRRHKGIFYVCFVANDTKKTYL
                     YTSKDITGPWKKQNIEGFYHDCSLLFDDDDRIYIVYGNAEIKLTELKADLSGPKPDGL
                     HRTIIKDSPGVRLGYEGSHIYKMNGIYYVFLIHWLADGSKRRVEACFCAKSLEGEFTG
                     GDVFDDDIGFQNAGVAQGGIVDTPDGDWYAMLFQDHGAVGRIPVLLPIRWENGFPIFG
                     ADKKTPLYIEPKSTRPDYKYRPLTGDDAFDYEPDENGTVHLKEFWQWNHTPDNSLWSM
                     TEKPGAFRLYSDKLCVNIVQAKNTLTQRMTGPACEMIVTVDGSGLKDGDYAGICALQG
                     CYGFIALTKASGKNYLVMTAKEMDPQESTWGEPGGDQKPGKEFERLPVTDDRITLKIR
                     ADFKDGIDEAEFYYLDKTKWNKLGITHKLYFRLDHFVGCRVGLFLYSTMAAGGRADFD
                     HFAYRTEGA"
     gene            1931..2551
                     /locus_tag="PLU43_00235"
     CDS             1931..2551
                     /locus_tag="PLU43_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO26862.1"
                     /translation="MKVLLMNGSPHEKGCTYTALSEAGKTLQENGVQTEFLWIGTKPV
                     AGCIACGACSGTGLCVFEDDVNVLLKKLDEYDGFIFGSPVYYAGPSGQLCAFLDRLFY
                     CGGKRMAGKPGAAVVSCRRGGASAAFDRLNKYFTICNMPVAASQYWNQVHGSRPEDVL
                     QDEEGMQTMRTLGQNMAWLLKSVAAGKENGTEVPVYEAKIATNFIR"
     gene            2585..3448
                     /locus_tag="PLU43_00240"
     CDS             2585..3448
                     /locus_tag="PLU43_00240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO26863.1"
                     /translation="MSIRVIASDLDGTLLNENHELDQIAYETLVRAEAAGIRFITATG
                     RNYPMTLDAFGKFDVRCEYILASGAETRNRQGDVVESLPLSSVKVREIADALEPTGIP
                     LIYNTAGKSFLTGETEKVRKGVIYEAENYYSKVGNPGFSKEELIQKILSRTVIVSAVD
                     DILQPVYKISINTGEKNAIGEMRKKLTDISGLAVASSFPNNIEITDEKAQKGPVLERY
                     IHQLGYTMEDVIVFGDSMNDYSMMCMEFGCTVAPENADPRIKAAAKMISPPNTEHGVV
                     RIIEKILEGTF"
     gene            3468..3836
                     /locus_tag="PLU43_00245"
     CDS             3468..3836
                     /locus_tag="PLU43_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26864.1"
                     /translation="MKKRMKGYLGKIDKILAEDAPETDYEELSREQLVQIGFFMHERL
                     VHLLVTLTFSVLTFAVFFLLLFQFFPALLLLFILLLVLLVPYIMHYYMLENGVQRLYG
                     QYDEMKRRINKGGKEIDHSL"
     gene            3820..4149
                     /locus_tag="PLU43_00250"
     CDS             3820..4149
                     /locus_tag="PLU43_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO26865.1"
                     /translation="MIIHYDTMEETVIPNFRGGEKEFITKMYTDDKNKIMRGKLKPGA
                     SIGFHCHDDGSEIVFVLSGTAKALYDDGEEALKAGICHYCPKGHSHSIINTGEEDFLF
                     FAVVACQ"
     gene            4168..4683
                     /locus_tag="PLU43_00255"
     CDS             4168..4683
                     /locus_tag="PLU43_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOO26866.1"
                     /translation="MSLKIVKGDITEAKTTVIVNAANCSLLGGGGVDGAIHRAAGPEL
                     LKECRTLNGCRTGQAKMTKGYRLAAQYVIHTPGPMWTGGRDGEAELLASCYRNSLKLA
                     QESGLTDISFPSISTGIYGYPLKAAATIAVNTIMEFPRMDVTMVCYDDRTKSAYENAY
                     EEYQQKSGSSF"
     gene            4815..5042
                     /gene="acpP"
                     /locus_tag="PLU43_00260"
     CDS             4815..5042
                     /gene="acpP"
                     /locus_tag="PLU43_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO26867.1"
                     /translation="MLERLKKMIEEQLNTDVEITEKTSFKEDLGADSLDLFELVMSME
                     EEFGVEIPTEDLEKISTVGDIMDYLKEHGAV"
     gene            5074..5997
                     /gene="fabK"
                     /locus_tag="PLU43_00265"
     CDS             5074..5997
                     /gene="fabK"
                     /locus_tag="PLU43_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOO26868.1"
                     /translation="MKTRVTELLSIEYPIIQGGMAWVAEHCLAAAVSNAGGLGLIGAA
                     SAPAEVVRDEIRKTKELTDRPFGVNVMLMNPNAREVAQVIVEEGVRVVTTGAGNPAKW
                     IPVWKKSGVKVIPVVASVAMAKMMEKAGADAVIAEGMESGGHIGSATTMTLVPQVVDA
                     VKIPVIAAGGIADGRGAAAAFMLGAEAIQMGTRFVVAKESIVHPDYKERILKAKDIDS
                     EVTGRSTGHPIRVLRNQMTKEYLQMEKEGVTFEELERLTLGSLRKAVMEGDIKNGSLM
                     AGQIAGLVRKEGSCEDIIKEIMQDAEKLLGA"
     gene            6037..6951
                     /gene="fabD"
                     /locus_tag="PLU43_00270"
     CDS             6037..6951
                     /gene="fabD"
                     /locus_tag="PLU43_00270"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOO26869.1"
                     /translation="MKKIVYMFPGQGVQYVGMGKAFYDHFAACKTIFQTASAVTGLDL
                     EALCFEANEKINITEYTQIAILTVEAAILKVMEEKGLRPDYTAGLSLGEYGALLASHV
                     LSYEDAFRLVRKRGIYMQQAAQTGGAMSAVLGLDADRIEAVCEQTEGIVSIANYNCPG
                     QIVITGETDAVAKASEELVKAGAKRIVPLHVSGPFHSPLLHGAGEQLAEELNKTNIHK
                     ITVPYVSNVTAQFVTEEDEIRGLLVQQVSSSVRWQQSMELLLAKGAEEFVEIGPGRTL
                     SGFMKKISRDAKVYHIEKPEDLESYVNR"
     gene            6938..>7367
                     /locus_tag="PLU43_00275"
     CDS             6938..>7367
                     /locus_tag="PLU43_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO26870.1"
                     /translation="MLTGKIALVTGASRGIGKEIAKTLADAGAYVYVNYNGSKQAAEE
                     TVLEIEKSGGRAEAIGCSVADFDAVKQMLGRIVEKQGRIDILVNNAGITKDNLLMKMS
                     EKEFEQVLAVNLKGAFHTIRHASRYFLKQKSGRIINISSVS"
BASE COUNT         2231 a         1318 c         2044 g         1774 t
ORIGIN      
        1 tgtggaggat acttgcatcg gctgtggtct gtgtgcggaa aaatgtccgg tgaatgcaat
       61 aaaaattcaa aacggaaagc ctgtctgggt cagggaacag tgtgtgatgt gtttgaaatg
      121 cctgcatcac tgccccaaat tttcgatcca gtatggaaga cacacaaaaa agcatgggca
      181 gtacagaaat ccaaatgtca gaggatgaca ggcataaaaa tacaatagtt ttacagggaa
      241 ggataattat atggaaaaca atccgattat ctattctgat tttccggatc cggatgtgat
      301 cagggtagac gatacatatt atatgatcag tacgacaatg cattttatgc cgggaggagt
      361 aatcctgcgg tcttatgatc tggttcactg ggagatattc acctatgtgt atgacagtct
      421 ggatgatacg ccggggcaga gactggaagg agctgcagct gtttatggaa aagggatgtg
      481 ggcagccacg cttcgccgcc ataaaggtat tttttatgtg tgttttgttg cgaacgatac
      541 gaaaaaaaca tatctgtata catcaaaaga tattacagga ccatggaaaa aacaaaatat
      601 agaaggcttt tatcatgact gttcattatt atttgatgat gatgacagga tctatattgt
      661 ttacggcaat gcggaaataa aactaacgga actgaaggca gatctgtcgg gtccaaaacc
      721 ggacggactg caccggacga tcataaaaga cagtccgggg gtacgattgg gatatgaagg
      781 ttcccatatt tacaaaatga atgggatcta ttatgtgttt ctgattcact ggcttgcaga
      841 cggctcaaag agaagagtgg aagcctgttt ttgtgctaaa tccctggaag gtgaatttac
      901 agggggggat gtatttgatg atgatattgg tttccagaat gcaggcgttg cgcagggcgg
      961 tatcgtagat accccggatg gagactggta tgccatgctg tttcaggatc atggggctgt
     1021 tgggcggata ccggtgcttc ttcctatccg ctgggaaaac gggttcccga tatttggtgc
     1081 ggacaaaaaa acgcctcttt atatagaacc aaagagcaca cgtccggact acaaatacag
     1141 accgcttaca ggcgatgatg cgtttgacta tgagccggat gaaaacggaa ccgttcattt
     1201 gaaggagttc tggcagtgga atcatacgcc ggataacagc ctttggtcca tgacggaaaa
     1261 gcctggggca ttccggctgt attcggataa gctgtgtgta aatattgtac aggcaaaaaa
     1321 tacattaaca cagcgaatga caggtcctgc ctgtgaaatg atagtaacgg tagacggaag
     1381 cggtttaaag gacggcgatt atgccggtat ttgcgcactg cagggatgct atggttttat
     1441 tgcgctgaca aaggcatccg gtaaaaacta cctggtaatg actgccaaag agatggatcc
     1501 gcaggaaagt acatggggag agccgggagg agatcagaag cctggaaaag aatttgaaag
     1561 gcttcctgtg actgatgaca ggatcacact aaaaatcagg gcggatttta aggacggcat
     1621 tgacgaggct gagttctatt atttggacaa aacgaaatgg aacaaactcg gtattacgca
     1681 taaattatat ttcagacttg atcattttgt aggctgccgt gtgggcttgt tcctgtattc
     1741 gaccatggct gccggcggca gggcggattt tgatcatttt gcttaccgga cggaaggggc
     1801 ataatttata tacagatata tccggaacaa aggaaaatca agttcagagg agaagaatta
     1861 ccgccgtgga attgaaattt tctctgtttg gcgttaaaat aaaagaaata taaaacgccg
     1921 ggaggaaaaa atgaaagtat tattgatgaa cggaagccct catgagaagg gatgtactta
     1981 tacggcgctg agcgaagcag ggaaaacact gcaggaaaac ggcgttcaga cagaattttt
     2041 atggatcggt acaaagccgg tggcaggctg tattgcctgc ggagcctgca gcggcacagg
     2101 gctgtgcgtt ttcgaggacg atgtaaatgt acttttgaaa aaactggacg aatatgacgg
     2161 attcatattt ggttccccgg tctattatgc cggaccgagc ggacagctct gcgcttttct
     2221 tgacaggctg ttttactgcg gagggaaacg gatggcagga aagcccggag cggcggtcgt
     2281 atcgtgcaga cgcgggggcg ccagtgcggc gtttgaccgc ttgaataagt attttacgat
     2341 ctgtaacatg ccggtcgcag catcccagta ctggaatcag gtacatggaa gcaggccgga
     2401 agatgtttta caggatgaag aaggaatgca gacgatgcgt acgcttggtc agaacatggc
     2461 atggctgctc aaaagcgtcg ctgccggtaa ggaaaacggg acagaggtgc ccgtatacga
     2521 agcaaagatt gcgacgaatt ttataaggta aaggccacag atcaaaaatc agggagtgtg
     2581 actgatgtca attcgtgtca ttgcgtccga tctggacggg acattattaa acgaaaatca
     2641 tgagctcgat cagatcgcct atgagacgct tgtccgtgca gaagctgcgg ggattcgttt
     2701 tataacggcg acgggcagaa attatccgat gacactggat gcgtttggga aatttgacgt
     2761 aaggtgtgag tatattcttg cgagcggagc ggagacgaga aaccgtcagg gagacgtggt
     2821 cgaaagtctg ccgctttcgt ctgttaaagt ccgtgaaata gcagatgcac tggaacccac
     2881 aggtattccg cttatttaca acacggcagg aaaaagtttt ctgacgggcg agacggagaa
     2941 ggtcagaaaa ggcgttattt atgaggcgga gaattattac agcaaagtag gaaatccggg
     3001 attttcaaaa gaagagctta tacagaaaat cctcagccgg acagtgatcg tttcggcagt
     3061 ggacgatatt ttgcagccgg tatataaaat ttcgataaat accggggaga aaaatgccat
     3121 cggcgaaatg cgaaaaaagc tgacggatat ttcaggactt gccgttgcgt cttcttttcc
     3181 aaataatatt gagatcacgg atgaaaaagc gcagaagggt cctgttttgg agcgctatat
     3241 ccatcagctc ggctatacga tggaggatgt gatagtattc ggagacagta tgaatgatta
     3301 ttccatgatg tgcatggagt ttggctgtac ggttgcaccg gagaatgcag atccccggat
     3361 aaaagcagcg gctaaaatga tttctccccc caatacggaa catggtgttg tgagaataat
     3421 agaaaaaata ttagagggaa cattttgagc aggagaaagc tgttactatg aaaaaaagaa
     3481 tgaaagggta tttgggaaaa attgataaaa tccttgcaga ggatgcaccg gaaacggatt
     3541 atgaagagct ttccagggaa cagcttgttc agatcggatt ttttatgcat gagagacttg
     3601 tgcatctgct tgtgacactg acattttccg ttctgacatt tgcagtattc tttctgctgc
     3661 tgtttcaatt tttcccggca ctgctgcttc tttttattct tctgcttgtt ttgctcgttc
     3721 cttatattat gcattattat atgctggaaa acggcgtgca gaggctttat ggacaatatg
     3781 atgaaatgaa acgaagaatc aataaaggag gaaaggaaat tgatcattca ttatgatact
     3841 atggaagaaa cggtcatccc caattttcgc ggcggggaaa aagaatttat tactaaaatg
     3901 tatacggacg ataaaaataa aatcatgcgg gggaaactaa aaccaggcgc ttcgatcggt
     3961 tttcactgcc atgatgacgg cagcgagatc gtatttgtgc tgagcggaac ggcaaaggcc
     4021 ctgtatgatg acggggagga agcgttgaag gctggaatct gtcactactg tccgaaagga
     4081 cattcgcaca gtattatcaa tacgggagaa gaagattttc tgttttttgc agtcgtagcc
     4141 tgtcagtgag aaatgggggc tggtaaaatg tcactgaaga tcgtaaaagg agatattaca
     4201 gaagcaaaaa ccacggtaat cgtaaatgcg gccaactgtt cactgcttgg agggggcggt
     4261 gttgacgggg caatacaccg ggcagcagga cctgagcttt tgaaggaatg caggacatta
     4321 aacggatgca ggacagggca ggccaaaatg acaaaaggat atcgtctggc agctcagtat
     4381 gtgatccata caccgggacc gatgtggacc gggggaaggg acggtgaagc ggaactgctt
     4441 gcctcctgtt atcggaacag tttaaagctt gcacaggaaa gcgggctgac cgatatttcg
     4501 tttccgtcga tcagtaccgg aatctatggg tatccgctaa aagcggcagc gacgatcgct
     4561 gtaaatacga tcatggaatt cccgcggatg gatgtaacaa tggtctgcta tgacgacaga
     4621 acaaaatccg cttatgaaaa tgcctatgag gaatatcagc agaaatccgg ttcatctttt
     4681 taaaagatgg gccggatttc tatttatttt acttgacaaa aaagaaaatc aggcataata
     4741 ttttgaatgt caaacaattt gaatatcaaa ctatttgaca ttcaaataaa attgaaaaac
     4801 aaaggagata aaaaatgctg gaaagattaa aaaaaatgat cgaagagcag ttgaatactg
     4861 atgtggaaat tacggaaaaa acttctttta aggaggatct tggagcggat tcccttgact
     4921 tatttgagct tgtaatgtca atggaggaag aattcggtgt ggaaatacca acggaagatc
     4981 tggaaaagat cagtacggtc ggagacatta tggattattt aaaagaacac ggcgcagtat
     5041 gatctgaaat cctcaaatcg acaggagagc atcatgaaaa caagagtaac agaacttcta
     5101 tcaatcgaat atccgatcat tcagggcgga atggcatggg tcgcagaaca ttgtcttgca
     5161 gcggctgttt caaatgccgg aggacttgga ctgatcggag cggcaagcgc accggcagag
     5221 gtcgtgcgtg atgagatcag aaaaacaaaa gaattgacgg accggccgtt tggggtcaat
     5281 gttatgctga tgaatccaaa tgcgcgggag gttgcacagg taattgttga agaaggagtc
     5341 agggtcgtaa cgacaggtgc cggaaatccg gcaaaatgga ttcctgtatg gaaaaaatcc
     5401 ggtgttaaag tgatccctgt tgttgcgagc gttgccatgg caaaaatgat ggaaaaagca
     5461 ggcgcggatg cggttattgc agaaggaatg gaatccggcg gacatattgg ctctgccaca
     5521 acgatgacgt tagtgccgca ggtagtggat gccgtaaaaa ttccggtcat tgcagcgggc
     5581 ggaattgcag acggaagggg tgctgcggct gcgtttatgc ttggagccga agctattcag
     5641 atgggaaccc ggtttgtagt ggcaaaggag tccattgttc atcctgatta taaagaacgg
     5701 atcttaaagg caaaagatat tgattccgaa gtgacgggaa ggtcaacagg tcatccgatc
     5761 cgtgtgctgc gcaatcagat gacaaaggaa tatctgcaga tggaaaaaga aggggtaacg
     5821 tttgaagaac tggaacggct tactctgggt tctctgcgga aagctgtcat ggaaggggat
     5881 attaagaatg gaagcctgat ggcagggcag atcgcaggct tggtcagaaa agaaggttcc
     5941 tgtgaagata ttataaaaga aatcatgcag gatgcagaga aactgcttgg tgcatgagaa
     6001 atgcagcggg agaaacagcg gaaggagagc agcactatga aaaaaatcgt ttatatgttt
     6061 ccgggacagg gagtacagta tgtcggaatg ggaaaggctt tttacgacca ttttgccgca
     6121 tgtaaaacaa tatttcaaac ggcgtcggcg gtaacgggtt tagatctgga ggctctctgc
     6181 tttgaagcaa atgaaaaaat caatataaca gaatatacac agatcgccat cttgactgta
     6241 gaagcggcaa ttctgaaagt gatggaagag aaaggattac gccctgatta taccgcgggc
     6301 ctgagtcttg gtgaatacgg agcactgctc gcatcgcatg ttttaagcta tgaggatgca
     6361 ttccgtcttg tcagaaaacg cgggatctat atgcagcagg cggcgcagac aggcggcgca
     6421 atgtcggccg tacttggact ggatgcggac cgtatagaag cggtctgtga acagacggaa
     6481 gggatcgtct cgatcgcaaa ttataactgt ccgggacaga tcgttataac cggagaaacg
     6541 gatgcggtgg caaaagcatc ggaggaactt gtaaaggccg gggcaaagcg gatcgtaccg
     6601 ctgcatgtga gcggtccttt tcattcaccg ctgctgcatg gagctggtga acagctggca
     6661 gaggaactca ataaaacgaa tattcataaa attacagttc catatgtttc taacgtaacg
     6721 gcgcagtttg tgacagaaga agatgaaata agaggccttc tcgtacaaca ggtttcctct
     6781 tccgtacggt ggcagcagag catggaactg ctgcttgcga aaggtgcgga agaatttgtg
     6841 gagatcggac ccgggcgtac gctgagcggg tttatgaaaa agatcagcag agatgcaaag
     6901 gtatatcata ttgaaaaacc ggaggatttg gaaagctatg ttaacaggta aaattgcact
     6961 tgtaacaggt gcttcaagag gaattggaaa agagatcgca aagacgcttg cggacgccgg
     7021 tgcttatgta tacgttaatt ataacggctc aaaacaggct gcagaagaaa cggttcttga
     7081 aattgaaaag agcggcggca gggcggaagc aatcggatgc agtgttgcgg attttgatgc
     7141 ggtcaaacaa atgctgggcc gtattgttga aaaacaggga aggatcgata ttcttgtgaa
     7201 caatgcggga attacaaaag ataatcttct gatgaaaatg tcagagaagg aatttgagca
     7261 ggtgctggcc gttaatttaa aaggtgcctt tcatacgatc cggcatgcgt ccagatattt
     7321 tctgaaacag aagagcggac ggatcataaa tatttcttcg gtatccg
//
LOCUS       DAOSYE010000008         7134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_6838_length_7134_cov_3.258511, whole genome shotgun sequence.
ACCESSION   DAOSYE010000008 DAOSYE010000000
VERSION     DAOSYE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6838_length_7134_cov_3.258511"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLU43_00280"
     CDS             complement(<1..223)
                     /locus_tag="PLU43_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOO26871.1"
                     /translation="MLLKAAINDGKKIRIIGDYDIDGVCAAYILFRGLTHCGANVDVR
                     LPDRVRDGYGLNETMIRDAVNDGVGFMLTC"
     gene            complement(214..417)
                     /locus_tag="PLU43_00285"
     CDS             complement(214..417)
                     /locus_tag="PLU43_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26872.1"
                     /translation="MKRWVVAAKRSDFNKTAEKFHISPMLARIIRNRDVIGDAAVDRF
                     LNGTPEDLHAPSLMKDMMRQPCF"
     gene            complement(516..2798)
                     /gene="secD"
                     /locus_tag="PLU43_00290"
     CDS             complement(516..2798)
                     /gene="secD"
                     /locus_tag="PLU43_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOO26873.1"
                     /translation="MKKRNAIITLAVLILLIAGFGYMTMFGVGTEKTGAAEQIKLGLD
                     LAGGVSITYQAVGDEKPSSEDMSDTIYKLQQRVENYSTEAQVYKEGDDRINIEIPGVN
                     DANAVLEDLGKPGSLYFISQTDSDGNENYQYTGQVDENGKAIYELTKTLDELEADGSI
                     VMTGTDVKAAQAGSYKNDMNNTEYVVELTLNEDGTQKFADATTAAYASGESIMIYYDG
                     KAVSVPRVESAITGGTAQITGMSDYEEAENLASTIRIGGLKLELEELRSNVVGAQLGT
                     EAISTSIKAGMVGFAIVILFMIGVYFVPGVAASLALCLYIVLMMVCLNLFDITLTLPG
                     IAGIILSVGMAVDANVITFARIREEIASGKTVRSAIKIGYEKALSAIIDGNVTTLIAA
                     IVLGILGSGSIKGFASTLALGIILSMFTAVVITKLILNAFYTLGIQNEKFYGKAKDRK
                     NINFLSGKFIFMGASIAVILAGFIVMGVNSARGVHALNYSLEFLGGTSTNVTFNEDMP
                     LEDIDAQVVPLIEEITGDGNVQVQKVAGSTEVIFKTRTLSVEERQAFTDAMTNNFSVN
                     AEKITTETISSVISSEMQKDAVIAVLVATICMLIYIWFRFKDIRFGASSVLALTHDVL
                     VVLAFYAFVRISVGSTFIACMLTIVGYSINATIVIFDRIRERLSEMKSKDSLEEVVNG
                     CVNQTLSRSIFTSLTTFIMVAVLYLFGVSSIKEFALPLMAGIICGTYSSVCLASAIWY
                     LLRTKAVKKAPVKAVKGKKK"
     gene            complement(2881..4314)
                     /gene="scfB"
                     /locus_tag="PLU43_00295"
     CDS             complement(2881..4314)
                     /gene="scfB"
                     /locus_tag="PLU43_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOO26874.1"
                     /translation="MIHQYKNNGFNIVMDVNSGSIHVVDDLVYTMIQALDSLDDENLF
                     RCVLDNDEIKAEPFEKPGKILKETFPDVPEEDLREAYAEISELIEAETLFSEDIYEQY
                     VEEFTNRESVVKALCLHIAHDCNLACRYCFAEEGEYHGKREIMSYEVGKAALDFLIQN
                     SGGRYNLEVDFFGGEPLMNFQVIKKLVAYGRSMEKPHHKKFRFTLTTNGVLLNDEVIA
                     FANKEMSNLVLSIDGRREVHDLMRPHRGGQGSYDEILPKFIKAAESRNQMDYYVRGTF
                     TRNNLDFSNDAIHLADLGFEQISIEPVVADEKEEYAIRREDIPVILEQYDILAQELLR
                     RKKEGRGVNFFHFMIDLSGGPCVAKRLSGCGSGTEYLAVSPSGELYPCHQFVGIPEFL
                     LGTVDTGVTNMECRDKFKSCNVYAREKCRTCFAKFYCSGGCAANAYNFSGSIMDTYDI
                     GCELQKKRIECAIMMKAAELTDDSAEN"
     gene            complement(4377..4520)
                     /gene="scfA"
                     /locus_tag="PLU43_00300"
     CDS             complement(4377..4520)
                     /gene="scfA"
                     /locus_tag="PLU43_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOO26875.1"
                     /translation="MKHIRTLNTRDFKKSMKKGGCGECQTSCQSACKTSCTVGNQSCE
                     KVK"
     gene            complement(4620..5729)
                     /locus_tag="PLU43_00305"
     CDS             complement(4620..5729)
                     /locus_tag="PLU43_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HOO26876.1"
                     /translation="MSESTDFENIKTAERTQTMEETDRKCPQCGGTMDFDPETGGLHC
                     PYCDYKEEIREVPQEPEKAKELDFSTAEQTGNCDWGMNQKTVICKSCGAQSVYDALEI
                     ASVCPYCGSNQVMEANDVSTLAPGGVVAFKITAKQAGSAFYKWIKRKWFCPRAAKESA
                     KAEAFQGIYLPYWTFDADTTTNYTAEYGINREVRRGQETKTVTDWHRTSGVFVQFIND
                     QLVSGTTNHEESTLHRIEPYDTEDNKAYKPEYIAGFAAERYSLGLKDAWEKARDFIKS
                     RLEALIENKISDEHHADEVKNVVMDTAYRNITYKYLMLPVWISSFTYKGKVYQFMVNG
                     QSGKVGGHSPISAIRVAVAVLIGLAAVALICWLSN"
     gene            5976..>7134
                     /locus_tag="PLU43_00310"
     CDS             5976..>7134
                     /locus_tag="PLU43_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOO26877.1"
                     /translation="MDTDGVIQCISLVLLLGFSAFFSSAETALTTVNRVKLQSMAEKG
                     SKRAATTLKLLDCPAKMLSTVLIANNIVNISASSLATTIALKLWGSSAVAIVTGVLTL
                     LVLIFGEITPKTAASLYSEKLSIAYGGTILFLSRLLTPVIYAVDKLSNGLLKLFRIDA
                     AKARSAMTEYELRTLVDVSHEDGVIESEERQMIYNVVDFGDSLAKDIMIPRIDMSCVN
                     VDATFEELKEVFYEEKYTRIPVYENSTDNIIGIVNMKDVLFYEDPAQFHIRDIMREAS
                     FTFEYKKTSELMLEMRENSISMILVLDEYGATVGLISLEDLLEEIVGEIRDEFDENEK
                     DLIQDIGAGEYLVEGSLKLDDINDALDLSLDSEDYDSIGGLIIEKLDRLPEA"
BASE COUNT         1870 a         1869 c         1328 g         2067 t
ORIGIN      
        1 cacatgtaag cataaatccg acaccgtcat ttacggcatc cctgatcatt gtttcattga
       61 gtccgtagcc gtccctaact ctgtccggca gacgcacatc cacatttgcc ccgcagtgtg
      121 tcaatccgcg gaataaaata taagcggcac agacaccatc aatatcataa tctccgataa
      181 tgcgaatctt tttaccgtca ttgattgctg cctttaaaag cacggctgcc gcatcatatc
      241 tttcatcaga gaaggggcat gcagatcttc aggcgttccg ttcaaaaaac gatcgactgc
      301 cgcatccccg atcacatccc tgttcctgat aattctggcc agcatcgggg aaatatgaaa
      361 tttttctgcc gttttattaa aatcagaccg ttttgcggcc acaacccagc gtttcataca
      421 ttttccatct cctgttccta tgttaaaagt tttcaaaaaa ggactgtccg gtaagacagt
      481 ccttttgatt ttcaattatt gcatgctgca gtttattact tttttttgcc ctttaccgcc
      541 ttgaccggtg ctttcttaac agccttggtc cgcagcagat accagatcgc acttgcaaga
      601 catacggaag aatatgttcc gcatataata cctgccatca gcggcagcgc aaattccttg
      661 atcgagctga cgccaaacag atacagcaca gcaaccataa taaatgttgt cagggacgta
      721 aaaatacttc ttgataatgt ctggttcaca catccgttca cgacctcctc cagactgtcc
      781 tttgacttca tctctgaaag ccgttccctg atcctgtcga aaataacgat cgttgcattg
      841 atggaatacc cgacgatcgt cagcatacag gcaataaacg tactgccgac agaaattctg
      901 acaaacgcat aaaaagcaag tacaacaagc acatcatgtg tcagtgcaag aacagaactg
      961 gcaccaaacc ggatatcctt aaaccggaac cagatataga tcagcataca gatcgtcgca
     1021 acaagtacgg ctatgaccgc atccttctgc atttccgaac tgatgaccga gctgatcgtc
     1081 tccgttgtga tcttttcggc attgacggag aaattattcg tcatggcatc cgtaaacgcc
     1141 tgtctctctt ctacggataa ggtccgcgtc ttaaaaataa cttcagtgct tcctgctacc
     1201 ttctgcacct gaacatttcc gtcacccgta atctcttcga tcagcggaac aacctgtgca
     1261 tcaatatcct ccagcggcat atcttcatta aacgttacat ttgtcgacgt accgcccaaa
     1321 aactcaagac tgtaattcaa ggcatgcact ccgcgtgcgc tgttgacacc cattacgata
     1381 aagccggcca gaatgaccgc gatcgaagcc cccataaata taaattttcc ggaaagaaaa
     1441 ttaatgttct ttctgtcctt tgcctttccg taaaactttt cattctgaat acccaatgta
     1501 taaaaggcat tcaatatcaa tttcgtaatt acaactgccg taaacatgga aagaataata
     1561 ccaagggcaa gtgttgaagc aaaccctttg atcgagccgc tgcccagaat ccccagtacg
     1621 atcgccgcga tcagtgttgt tacatttccg tctatgatag cagataacgc cttttcatag
     1681 ccgatcttga ttgcggaacg cacggttttc ccggatgcga tctcctctct gatacgtgca
     1741 aacgtaataa cattggcatc aaccgccata cctacggaaa ggataatacc cgcaatacca
     1801 ggaagtgtca gcgtaatatc aaacagattc agacatacca tcatcagtac gatatacagg
     1861 cataatgcaa gggatgccgc tactccagga acaaaataaa caccgatcat aaaaaggatc
     1921 acaatggcaa atccgaccat acctgcctta atactcgtag aaatggcttc cgtaccaagc
     1981 tgtgcaccca cgacattcga acgcagctcc tcgagttcca gcttcaggcc accgattcga
     2041 atcgtagaag ccagattttc tgcctcttca taatcgctca tcccggtaat ctgtgccgtt
     2101 ccgcccgtga ttgccgattc tactctcgga acactgacag ccttgccgtc gtaatagatc
     2161 atgatcgact ctccggacgc ataagctgct gttgttgcat cggcaaattt ctgcgtacca
     2221 tcctcgttca atgtcaattc aacaacatat tccgtattgt tcatgtcatt tttataactg
     2281 cctgcctgtg ccgcctttac atccgttccc gtcattacga tgcttccgtc tgcttccagc
     2341 tcatccagcg tttttgtcaa ttcataaatc gccttgccgt tttcgtcgac ctgaccggta
     2401 tactgataat tttcatttcc atcactgtcg gtctgactga taaaatagag ggaaccgggt
     2461 tttcccagat cctcaagtac agcattggca tcattcacac ccggaatttc gatgttgatc
     2521 ctgtcatcgc cctccttgta tacctgtgct tccgtactgt aattttccac acgctgctgc
     2581 agcttgtaga tcgtatcgct catatcttcc gaagacggct tttcgtcccc gactgcctga
     2641 tatgtgatgc tgacgccgcc ggcaaggtca agtcccagct tgatctgctc tgcagcaccc
     2701 gttttttccg taccaactcc aaacattgtc atataaccaa agccggctat caacaaaatc
     2761 agtacggcaa gtgtaatgat cgcattccgt ttcttcatta cgatttctcc ctttctgctt
     2821 tctctctgtt tatttcataa ctgtcctgat atccagttaa atcatacgaa ctgccgcttt
     2881 tcagttttcc gcgctgtcat ccgtaagctc tgcggccttc atcataatgg cacattcgat
     2941 ccgctttttc tgaagctcac agccaatatc atacgtatcc ataatactgc ctgaaaaatt
     3001 atacgcattt gccgcacagc cgccgctgca gtaaaacttg gcaaaacatg tcctgcattt
     3061 ttctctggca taaacattac aggacttaaa tttatccctg cattccatat tcgtaacgcc
     3121 ggtatccaca gttccaagca gaaattcggg aattccgaca aactgatgac acggatacag
     3181 ctctcccgac ggggaaacag ccagatattc cgtaccgctt ccacatcctg acaacctctt
     3241 tgccacacat ggtccgcctg acagatctat cataaaatga aagaaattaa cgcctcttcc
     3301 ttcttttttt cttcgcagca attcctgcgc aagaatatca tactgctcta aaataaccgg
     3361 aatatcttct ctgcggatgg catactcttc tttttcatcc gccacaaccg gttctatcga
     3421 tatctgttca aagccaagat cagcaagatg tatcgcatca ttggaaaaat ccagattatt
     3481 tctcgtaaat gtgcccctga cataatagtc catctgattt ctgctttccg cagctttgat
     3541 gaacttcggc agaatctcat catagcttcc ctgtccgccc ctgtgcgggc gcatcaggtc
     3601 atgcacttcc ctgcggccgt ctatgcttaa aacaagattg ctcatctcct tattcgcaaa
     3661 tgcgatcact tcgtcattca acaggactcc attcgtcgtc agggtaaacc gaaacttttt
     3721 atgatgcggt ttctccatac tcctgccata agcaacaagc ttttttatga cctggaaatt
     3781 cataagcggt tccccgccaa aaaaatccac ttcaagatta tagcggccgc cggaattctg
     3841 gataagaaaa tccaatgccg ccttcccgac ctcatagctc atgatctccc gctttccgtg
     3901 atattccccc tcttccgcaa agcaatacct gcatgcgagg ttgcagtcat gcgcaatatg
     3961 cagacaaaga gcctttacaa cactctctct gtttgtaaat tcctcaacat actgctcata
     4021 aatatcctcg gaaaaaagcg tttccgcctc gatcagctct gaaatttccg catacgcctc
     4081 cctaagatct tcttccggga catcgggaaa tgtttctttc agtatttttc ccggcttttc
     4141 aaatggctcg gctttaatct catcattatc taatacgcac ctgaacaggt tctcatcgtc
     4201 aagcgaatcc agcgcctgaa tcatcgtata gaccaggtca tccacaacat gaatggaacc
     4261 gctgtttaca tccatcacga tattaaatcc gttattttta tattgatgta tcaatttgtt
     4321 tcctcaattc cgaataataa gcagcgacct ctgccggcag ctgcttaact tttttcttat
     4381 tttacttttt cgcagctttg atttcctacc gtacaggaag tcttgcaggc tgactggcag
     4441 gaagtctggc attcaccgca tccgcctttt ttcatggatt ttttaaaatc tcttgtattt
     4501 aatgtccgaa tatgcttcat atttccgctc ctccttataa aaacgctcag ataagtatac
     4561 cataaaccaa ttctattgaa aagtgttttt tacaccatga ttttgcaatg agccgaccgc
     4621 taatttgaca gccagcagat cagcgcaaca gctgcaagac cgatcaatac cgcaacagcg
     4681 accctgatcg cggaaatcgg tgaatgtccg cctactttac cggactgacc attcaccata
     4741 aactgataga cctttccttt gtatgtaaaa cttgagatcc atacaggaag catcagatac
     4801 ttataggtaa tgttccggta cgccgtatcc attacgacat tttttacttc atccgcatgg
     4861 tgttcatccg aaattttatt ttcgatcagc gcttccagac ggctttttat aaaatccctg
     4921 gccttttccc acgcatcctt caagccaagc gaatatcgtt cggccgcaaa cccggctata
     4981 tattccggtt tatatgcctt attatcttcc gtgtcgtacg gctcgatcct gtgaagcgta
     5041 ctttcctcat gatttgttgt gcccgataca agctgatcat tgataaactg gacaaaaaca
     5101 ccggatgtac ggtgccagtc tgttacagtt ttcgtttcct ggccacgcct tacttctctg
     5161 ttgatcccat attctgccgt ataatttgtc gttgtatccg catcaaatgt ccagtacgga
     5221 agatagatcc cctgaaaagc ttctgctttc gcactttcct ttgccgctct cggacaaaac
     5281 cattttcttt ttatccattt ataaaatgca ctgcctgcct gctttgccgt gattttaaat
     5341 gccacgacgc cgcccggcgc aagtgtcgaa acgtcatttg cctccatgac ctgattggac
     5401 ccgcaatagg gacaaacact tgcgatctcc agtgcatcat ataccgactg cgccccacag
     5461 gatttgcata taactgtttt ctgattcatc ccccagtcac aatttccggt ctgctctgca
     5521 gtcgaaaaat ccagttcttt agctttttcc ggttcctgcg gcacttctct gatttcctct
     5581 ttataatcac agtacggaca gtgcagaccg cccgtttccg gatcgaaatc cattgttcct
     5641 ccgcactgag gacatttacg gtctgtttcc tccatcgtct gtgtccgctc cgccgtctta
     5701 atattttcaa aatctgttga ttcactcatt tttcaccctc cctgttacca tttcatactt
     5761 aaatttcagc cgcatatgat tttttttatc gtatcacaat ctgtccgcga agtccaaccg
     5821 catttgaccc tgtccgctta tatttgttaa atgtttgtaa tatttactat cttttcttga
     5881 catgatacag aactgtacgt tatcatttat attgaaaaat tggattctgt actgatttcc
     5941 ttaattagta caaacaaaga atggaggctg ttttcttgga taccgatggt gtcatacaat
     6001 gtattagttt agttttatta ttaggttttt ccgctttctt ttcttcagcg gagaccgcac
     6061 tgacgaccgt aaaccgcgtg aagctgcagt caatggcgga aaaaggcagc aaacgcgccg
     6121 ctacaacact gaaactgctt gactgccctg ccaaaatgct gagtacggtc ctgattgcaa
     6181 ataatatcgt caacatttcc gcctcatctc tcgccactac gattgcctta aagctttggg
     6241 gcagcagcgc cgttgcgatc gtaaccggag tacttacatt attggtttta atattcggcg
     6301 aaatcacacc caaaaccgct gcatctcttt acagcgaaaa attatcgatc gcctatggcg
     6361 gaacgattct gtttttaagc agactgctga cacctgttat ctatgcggtc gataaattat
     6421 caaacggact gctgaagctt tttcgcatag acgcagccaa ggcacgcagt gccatgacgg
     6481 aatatgaact gcgtacactt gtggatgtca gccacgagga cggagtcatt gaaagtgaag
     6541 aacggcagat gatctataat gttgtggatt ttggagattc tctcgcaaaa gatattatga
     6601 taccgcgtat cgacatgtcc tgtgtgaatg tcgatgccac ttttgaagaa ttaaaagaag
     6661 tattttatga agaaaaatat acgcgtattc ctgtatatga aaattctacg gataatatta
     6721 tcgggatcgt caatatgaaa gacgttctct tctacgaaga tccggcacag tttcatatcc
     6781 gtgatatcat gcgcgaggcc agttttactt ttgaatataa aaaaacatcc gagcttatgc
     6841 tggagatgcg cgaaaattct atcagcatga ttcttgttct tgacgaatac ggcgctacgg
     6901 tcggtcttat cagcctggaa gacctgctgg aggagatcgt tggagagatc agggatgaat
     6961 tcgatgaaaa cgaaaaagac ctgatccagg acatcggcgc tggggaatac cttgtggaag
     7021 gctcactgaa gcttgacgat atcaatgacg ctctggacct ttcgcttgac tccgaagact
     7081 atgattccat cggcggactg atcatcgaaa agctggaccg tcttcctgaa gccg
//
LOCUS       DAOSYE010000009         7000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7008_length_7000_cov_2.991217, whole genome shotgun sequence.
ACCESSION   DAOSYE010000009 DAOSYE010000000
VERSION     DAOSYE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_7000_cov_2.991217"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PLU43_00315"
     CDS             complement(<1..1281)
                     /locus_tag="PLU43_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO26878.1"
                     /translation="MSKKIKFKGQIRSYIQWPLRFTILIAVFNICIYFVDIYAGFVMS
                     AFAVIYSSIALFLYFRNKAIVINELVSFATQYGQIQKQLLRDLELPYALLDETGKVIW
                     TNEAFEHTLHVKKGYRKSIMSLIPKMHKGILPGIESETELMIMHEEKDYRVSMKKVSM
                     KAMLATSTIVDTFDYDSYLIAFFMFDETEINRLQRENDEQKLATGLIYIDNYDEALES
                     VEEVRRSLLVALIDRKINKYFSTMDALVKKLEKDKYLVILQKKYLKTLTEKRFDLLED
                     VKTVNIGNEMALTLSIGFGIETGSYQHNYDYARTAIDLALGRGGDQAIIKRPGDITYY
                     GGKSQKTEKNTRVKARVKAHALKEIIESNDKIIVMGHKILDVDAFGAAIGIYRAAKSF
                     GKKTHIVVNEITTSIRPLMDGLLAAGDYEEDIFLN"
     gene            complement(1515..3149)
                     /locus_tag="PLU43_00320"
     CDS             complement(1515..3149)
                     /locus_tag="PLU43_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOO26879.1"
                     /translation="MPITELLERNSREYGSDICLVEINPEVKEDHRVTWKEYELIEPT
                     PQTHYRREITWSVFDEKANRFANLLISRGVKKGDKVGILMMNCLEWLPIYFGVLKTGA
                     LAVPFNFRYSSDEIMYCAKLAKIDVLVFGPSFIGRIEEIADELIKRRLLIYVGENCPS
                     FAEDYNSHTANCSSRKPDIVITDEDDAAIYFSSGTTGFPKAILHKHLALVHSCKVEQN
                     HHGQTREDVFLCIPPLYHTGAKMHWFGSLISGSRAVLLKGTKPEFILDAVSKEHCTIV
                     WLLVPWAQDILEAIESGHVKLSDYELSQWRLMHIGAQPVPRSLIKKWKEYFPNHQYDT
                     NYGLSESIGPGCVHLGVENIEKVGTIGKAGYGWETKIVDESRKEVKRGDVGELAVRGP
                     GVMVEYYHDRKATEEVLSDGWLYTGDMAKEDEDGFILLVDRKKDVIISGGENIYPVQI
                     EDFIRTNEAVHDVAVIGLPDKRLGEISAAIVELKQGYILTEEDMDEFCHQLPRYKRPH
                     KFIFADIPRNPTGKIEKPKLRELYGGKGLIEAQNIG"
     gene            complement(3323..3601)
                     /gene="rpsR"
                     /locus_tag="PLU43_00325"
     CDS             complement(3323..3601)
                     /gene="rpsR"
                     /locus_tag="PLU43_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOO26880.1"
                     /translation="MAYNRLQSDSDRSGSPMRRRGGGMRRRKKVCVFCGKDKVIDYKD
                     VNLLKRYVSERGKILPRRITGNCAKHQRALTVAIKRARHIALMPYVMD"
     gene            complement(3624..4064)
                     /locus_tag="PLU43_00330"
     CDS             complement(3624..4064)
                     /locus_tag="PLU43_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOO26881.1"
                     /translation="MNKVILMGRLTRDPDVRYSSGDSATAVARYSLAVDRRFKRNNDD
                     QTADFINCVAFGRSGEFAEKYFRKGTKVLVTGRIQTGSCTNRDGQKVYTTDVVVEEQE
                     FAESKAAAGNGEGGYVPARQEPSAEINDGFMNIPDGIDEELPFN"
     gene            complement(4083..4370)
                     /gene="rpsF"
                     /locus_tag="PLU43_00335"
     CDS             complement(4083..4370)
                     /gene="rpsF"
                     /locus_tag="PLU43_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOO26882.1"
                     /translation="MSKYELAVVLNAKIEDEERAAAMEKVKGYIARFGGTVTNVDEWG
                     RKRLAYEVQKMKEGYYYFIQFDAETTAPIEIENRVRIMENVIRYLCVKQEA"
     gene            complement(4474..4668)
                     /locus_tag="PLU43_00340"
     CDS             complement(4474..4668)
                     /locus_tag="PLU43_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOO26883.1"
                     /translation="MPEKLVYDVGDIVKLKKKHPCGSFEWEILRVGADFRLKCLGCGH
                     QIMIARKLVEKNTKELRKKA"
     gene            complement(4682..5404)
                     /locus_tag="PLU43_00345"
     CDS             complement(4682..5404)
                     /locus_tag="PLU43_00345"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOO26884.1"
                     /translation="MKELETLQKWIFDSNNIVFFGGAGVSTESGIPDFRSVDGLYNQK
                     YEYPPETILSHSFYRSKTEEFYKFYRDKMLCTTAKPNKAHLKLSELEKAGKLKAVITQ
                     NIDGLHQAAGSKTVLELHGSVMRNYCEKCGKFFDEEYIIHSTGVPVCDACGGKIKPDV
                     VLYEESLNQKTLKDSMDYIKNADILIIGGTSLAVYPAAGLIDYYRGHKLVLINKSATP
                     MDRRADLLIQGSIGDVFGQIAV"
     gene            complement(5466..5882)
                     /locus_tag="PLU43_00350"
     CDS             complement(5466..5882)
                     /locus_tag="PLU43_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOO26885.1"
                     /translation="MNHYKYEEIEVGHTESFTVTVTEEAQNRFCEITGDVNPLHCDLV
                     YAKELGYNQCVVFGMLTASYLSALAGVYLPGERSLLHSVRTKFSKPVYAGDTLTVEGT
                     VAEKNDTFNLLIVKAVIRNQDGVKVCKAELQVGVRP"
     gene            complement(5912..6160)
                     /locus_tag="PLU43_00355"
     CDS             complement(5912..6160)
                     /locus_tag="PLU43_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO26886.1"
                     /translation="MTREQVFYRVQEIFRDVFDDDELMITGETNADDIEDWDSLEHIT
                     LIVAMEKEFHMKFDIREVNKLENVGEMIDLIEHRLREK"
     gene            complement(6193..>7000)
                     /locus_tag="PLU43_00360"
     CDS             complement(6193..>7000)
                     /locus_tag="PLU43_00360"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOO26887.1"
                     /translation="YGEILIPGKSEQEIFLSSYICHPSMANNECSGPALLIWLANYIK
                     QMPDRKYSYRLILIPETIGAITYLSRHLAEMKKNIIAGFNLTCVGDDRTYSLVHSRYA
                     NTLADKVLANILHDHYPNYDTYSYLKRGSDERQYQAPGVDLPLVCFCRSKYHVYPEYH
                     TSGDNLDIISPDGLAGSYAVMQKCLEALEKNAKYRVTCLCEPQLGKRGLVPTMSSKET
                     YRQTLALKDILAYADGTNDLFELSNLIEQPLPVVLPIIEKLLAAGLIEEC"
BASE COUNT         1770 a         1831 c         1232 g         2167 t
ORIGIN      
        1 attcaggaaa atatcttctt catagtcacc tgcggcaagc aggccgtcca tcagcggacg
       61 gatcgacgtc gtgatctcgt ttacgacaat atgtgttttc tttccgaagc tcttggctgc
      121 acgataaatg ccgattgccg ccccaaatgc atccacatcc agtattttat gtcccataac
      181 gataatttta tcattactct caatgatttc ttttaaggca tgcgctttta cccgtgcttt
      241 aactcttgtg ttcttttcag ttttctggct ctttcctccg taataggtaa tatcgcccgg
      301 tctttttata attgcctggt ctccgcctct tccaagcgca agatcgatcg ctgtccgcgc
      361 ataatcataa ttatgctgat aggaacccgt ttcaatacca aatccgatgc tgagtgtcaa
      421 tgccatctca ttaccaatat taactgtctt gacatcctca agcaggtcga aacgcttttc
      481 cgtcaatgtc tttaaatatt ttttttgcag aatgactaaa tacttatcct tttccagctt
      541 ctttacaaga gcatccatcg tggaaaaata tttgtttatt tttctgtcga taagcgccac
      601 aagcagtgaa cgtctgacct cctccacact ttccagtgct tcgtcataat tgtcaatgta
      661 aatcagcccc gtagcaagct tctgctcatc attttcccgc tgcagtctgt ttatttccgt
      721 ttcatcaaac ataaaaaaag caatcagata gctgtcatag tcaaacgtat ctacgatagt
      781 agatgtggca agcatcgctt tcatagagac ctttttcata ctgacgcgat agtctttttc
      841 ctcatgcatg atcatcagtt ctgtttcgga ttcaatccca ggaagaattc ccttatgcat
      901 tttagggatc agcgacatga tcgatttccg gtagcctttt tttacatgca gcgtatgttc
      961 aaacgcttca ttcgtccata ttaccttacc ggtttcatca agcagggcat acggcagctc
     1021 cagatcccgt aaaagctgtt tttgtatctg accgtactgt gtggcaaagc tgacaagttc
     1081 atttatgaca atcgctttat ttctgaaata caaaaacaag gcgattgaac tatagatcac
     1141 tgcaaatgca ctcattacaa agccggcata aatatcaaca aaataaatac agatattaaa
     1201 aaccgctatt aaaatcgtaa accttaaagg ccactgaata taagaacgga tctgaccttt
     1261 aaatttgatt tttttactca ttctgactcc tttggtactt ttcaccggac accggaccat
     1321 tcccgatacc gacgggaaag attcatttca ctattatagc atttttcagg aaagttctca
     1381 actaccataa ttggtcgata tttcttgaaa aacactccat tttgttttct gcagccgata
     1441 aatatgaaaa cacatcttta tgaaacagca aaagaacgaa ccgtcatctg gtccgttctt
     1501 ttactgttcg ttcgctatcc tatattctgc gcctcgatca gacccttgcc gccatacagt
     1561 tcacgcagct ttggcttttc gatcttcccc gttggatttc ttggtatatc tgcaaaaata
     1621 aacttatgcg ggcgtttgta tcttggcagc tgatgacaga actcatccat atcctcttcc
     1681 gtcagtatat atccctgctt cagttcaacg atcgccgcac tgatttctcc aagccgctta
     1741 tccggcaatc cgataacggc cacatcatgc actgcttcgt tcgtgcggat aaaatcctca
     1801 atctgcaccg gataaatatt ttcaccgccg ctgatgatca catccttttt tctgtcgaca
     1861 agaagaataa acccatcttc gtcctctttc gccatatctc ccgtatataa ccagccgtcc
     1921 gacaatactt cctccgttgc cttccggtca tgataatatt caaccataac acccgggcct
     1981 ctaaccgcaa gttcccccac gtctccgcgt tttacttcct tccggctttc atctacgatc
     2041 tttgtttccc agccgtaacc ggcttttccg atcgtaccga ccttctcgat attttcaacc
     2101 ccaagatgaa cacagccggg accgatcgac tcggaaagac cataattcgt atcatactga
     2161 tgattcggaa aatactcctt ccattttttt atcaggctgc gcggtacagg ctgtgcaccg
     2221 atatgcatca gtctccactg cgacagttca taatccgaca gcttcacatg gccgctctct
     2281 atggcttcca ggatatcctg tgcccacgga acaagcagcc atacgatcgt acagtgttcc
     2341 ttagataccg cgtccaaaat aaattccggc ttcgtacctt tcaaaagcac agccctgctg
     2401 cctgaaatca ggctgccgaa ccaatgcatc ttcgcaccgg tatgataaag cggaggaata
     2461 cataaaaata catcctcccg cgtctgtccg tgatgattct gctcaacctt gcaggaatgc
     2521 accagggcaa gatgtttatg taagatcgct ttcggaaatc ctgttgttcc ggaagaaaaa
     2581 taaatagccg catcatcttc atccgtaatc acaatatccg gtttcctgct tgaacagtta
     2641 gccgtatgtg aattataatc ttctgcaaag gaagggcagt tttctcctac atagatcaaa
     2701 agccttcttt ttataagttc gtccgcgatt tcttcaatac ggccgataaa tgacggccca
     2761 aacacaagaa catcgatctt ggcaagcttt gcacaataca tgatctcatc tgaggaataa
     2821 cgaaaattaa aaggaaccgc cagggccccc gtctttaata cgccaaaata aatcggcagc
     2881 cattccagac aattcatcat taaaataccg actttgtcac cctttttcac acctctgctg
     2941 attaaaagat tcgcaaagcg attggccttt tcatcaaaaa cactccatgt aatttctctg
     3001 cggtaatgtg tctgcggcgt cggctcgatc agttcatatt ccttccatgt tactctgtgg
     3061 tcctccttta cttcaggatt gatttccaca aggcagatat cactgccgta ttccctgctg
     3121 tttctttcca ataattccgt gattggcatt tgctgtaccc ctatctgatg attgctttcg
     3181 tgcacaaacg cgttcaagcg tttaattact aaagcttact ataccgtttt ctttataaaa
     3241 tgtcaatata gcctgctgct gccggaatgc cggcataaaa acagaccggc tgttctatag
     3301 ccggtcttgt ctattattat gatcaatcca ttacataagg catcaatgca atatgtcttg
     3361 ctctcttgat cgctactgta agagctctct gatgctttgc acagtttcct gtgatccttc
     3421 ttgggaggat ctttcctctc tcagacacat atctcttcag caaatttaca tctttataat
     3481 caattacctt atcttttccg caaaatacac aaaccttttt ccttctgcgc attccgcctc
     3541 ctcttctcct catcggagaa ccggatctgt cactatcgct ttgtagtcta ttgtaagcca
     3601 tcttaatcta cctcctgata aacttaatta aatggtaact cttcatcaat tccgtcagga
     3661 atattcatga agccgtcatt gatctctgcc gaaggttcct gtctggcagg cacgtaaccg
     3721 ccttcaccgt taccggcagc agctttgctc tcggcaaatt cctgttcctc cacgacaaca
     3781 tctgtcgtat agactttctg tccgtctctg tttgtgcagc ttcctgtctg aatacgtccg
     3841 gtaactaata cctttgtacc ctttctgaaa tatttttcgg caaactctcc tgacctaccg
     3901 aaagcaacac aattaataaa atcagctgtc tgatcgtcgt tatttcgctt aaatcttctg
     3961 tctaccgcca gagaatatct tgcaactgcc gtagcactat cccctgatga atatctgaca
     4021 tccggatccc tcgtcaatct acccataaga atcactttat tcatacgtac tctctctttc
     4081 tattatgcct cttgcttaac gcataaatat ctgattacgt tttccataat gcggacgcgg
     4141 ttttcgattt caatcggagc cgtagtttcg gcatcaaact gaatgaaata gtaataaccc
     4201 tctttcatct tctgaacttc ataggccaat ctcttcctac cccattcatc aacgtttgtc
     4261 accgtaccgc cgaatcgggc aatataaccc ttgactttct ccatggcggc cgctctttcc
     4321 tcgtcttcaa tcttagcatt aagaacaacg gctaattcat atttgctcat gctggtacct
     4381 ccttttggtc tctggccccc tgactgtgca gggagcaagg aaattatttc attacatcac
     4441 gagtttaaat gttaacatat atttcaagcc ttttcaagcc tttttcctca attccttcgt
     4501 gtttttttca acaagctttc tcgcaatcat gatttggtgt ccgcagccta aacatttcag
     4561 tctgaaatca gcgccgacac gaagaatttc ccactcaaag ctgccgcatg ggtgcttttt
     4621 cttgagcttt acaatatcgc ctacatcata tactaatttc tccggcatgt tccctcctgt
     4681 tctagacagc gatctgaccg aaaacatccc cgatactgcc ctgaataagc aggtcggccc
     4741 ttctgtccat tggtgttgcc gatttattga tcagtacgag cttgtgccct ctgtaataat
     4801 cgatcagacc cgccgccggg taaacggcaa gtgacgtacc tccgataatc aatatatcgg
     4861 catttttaat atagtccatt gaatccttca aagtcttctg attaaggctt tcctcataaa
     4921 gaacgacatc cggtttgatc ttcccgccgc atgcatcgca caccggaacg cccgttgaat
     4981 gaatgatata ctcttcgtca aaaaattttc cgcatttttc acagtaatta cgcatcacac
     5041 ttccgtgaag ctccagcaca gttttgctgc ccgcagcctg atgcagacca tcgatattct
     5101 gtgtaatgac cgctttcagc tttcctgcct tttcaagctc cgaaagcttc agatgcgcct
     5161 tattaggctt tgctgtcgta cagagcattt tatccctgta aaatttgtag aactcttccg
     5221 ttttgcttct gtaaaacgaa tggcttaaga tcgtttcggg cggatactca tatttttgat
     5281 tataaagtcc gtccacactg cggaaatccg gtattccgct ttccgtagat acaccggccc
     5341 cgccgaaaaa cacaatattg ttactgtcaa aaatccattt ttgcaacgtt tcaagttctt
     5401 tcatgaaaat tcctcccgtc ctttctcgtt ctgccaaggc tgtctgaacg ggccgccatg
     5461 ctgctctacg gacgcacacc gacctgcagc tcagctttac agacttttac tccatcctga
     5521 ttcctgatca ctgcctttac gatcaaaaga ttgaacgtat catttttctc ggcaaccgtt
     5581 ccttctaccg tcaatgtgtc acctgcgtag acaggttttg aaaactttgt ccgcacgctg
     5641 tgaagcaggc tcctttctcc aggaagatag acgcctgcaa gtgcggaaag ataggaagcc
     5701 gtcagcatcc cgaatacaac acactggttg tatcccagtt cctttgcata tactaagtca
     5761 cagtgcaggg gatttacatc gccggtgatc tcacagaacc ggttctgcgc ttcttcagtt
     5821 accgtcaccg taaaactttc tgtatgcccc acctcgattt cttcatattt gtaatggttc
     5881 ataaagtcct catttctata aaaattccga actatttttc acgcagtctg tgttcgatca
     5941 gatcgatcat ttcacctaca ttttccagtt tgttgacttc cctgatatca aatttcatat
     6001 ggaattcttt ttccattgca acgatcagtg taatatgctc caaagagtcc cagtcttcaa
     6061 tatcatccgc attcgtttcg ccggtaatca tcaattcatc gtcatcgaat acgtctctga
     6121 aaatctcctg tactctataa aatacctgtt ctctggtcat gtcctgtttt cctttcgctt
     6181 cattaagtat attcagcatt cttcaataag ccctgcggcc agcagcttct caatgatcgg
     6241 aagcacgacc ggcagcggct gttcgatcag attactcagt tcgaacagat cgtttgtccc
     6301 gtccgcatat gccagaatat ccttgagcgc cagtgtctgc cggtatgtct ctttgctgct
     6361 catcgtcggc acaagccctc ttttgccaag ctgcggctca cagagacatg tgacacggta
     6421 tttcgcattt ttttcaagtg cttccagaca tttttgcatg accgcatagc ttccggcaag
     6481 tccgtccggt gaaataatat ccagattgtc tccggaagta tgatattccg gatatacatg
     6541 atattttgaa cggcagaagc atacaagcgg cagatcaacg ccgggcgcct gatactgcct
     6601 ttcatccgat cctcttttca gataggaata cgtgtcatag ttcggataat gatcatgcag
     6661 tatattggca agtaccttat ccgccagtgt attggcatat ctggaatgca caagcgaata
     6721 ggttctgtca tccccgacac atgtcagatt aaatcccgca atgatatttt ttttcatttc
     6781 ggcgagatgg cggcttaaat aggtgatcgc cccgatcgtc tccggtatga gaatcaaacg
     6841 ataactatat tttctgtccg gcatctgttt gatataattt gcaagccaga tcagcagtgc
     6901 cggtccggag cattcattat ttgccatgga cggatgacag atatagcttg aaagaaaaat
     6961 ttcctgctca ctttttcccg gaatcagaat ctctccatac
//
LOCUS       DAOSYE010000010         6846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7210_length_6846_cov_3.551612, whole genome shotgun sequence.
ACCESSION   DAOSYE010000010 DAOSYE010000000
VERSION     DAOSYE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_6846_cov_3.551612"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLU43_00365"
     CDS             <1..535
                     /locus_tag="PLU43_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO26888.1"
                     /translation="LGTAEKATDILLSAFAKSAAAHDWTLKLIGPVKETFRPSIDLFC
                     EQYPQLKERVIFLGKITDKETLSKEYRKAKIFTLPSRSESFGIVLVEAANEGCYLITT
                     DMVPAGYDVNGNGRFGLVVPADNADALANAFSDLCGKNNSRDWNKEAQEISLFAQKTF
                     CWDTIIRQLYKAITECP"
     gene            complement(540..1403)
                     /locus_tag="PLU43_00370"
     CDS             complement(540..1403)
                     /locus_tag="PLU43_00370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO26889.1"
                     /translation="MREGSNVPVVSVITVCYNPGKSLIKTMESILAQTYAGFEYIIQD
                     GGSADGTAELLENYRLRFLEKGITLRCSMEKDGGIYDAMNKALQHAAGTWINFMNAGD
                     CFYSESVLSDIFEERDYANSAVLYGDAVECEYGRYYLFRKAFDSIESRMPFSHQSAFV
                     RKELLARLPFCTDYRIGADYDFLLTVYQKGFFFTDTNNIVCIVSKDGVSSMKLYDTFM
                     ETVHIWEGHGIHRYDERQMKKKAAELKIKQFVVDYFPKRIKKAIRRLQIIVRRQNASF
                     HIPVWEMENPG"
     gene            complement(1490..2206)
                     /locus_tag="PLU43_00375"
     CDS             complement(1490..2206)
                     /locus_tag="PLU43_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO26890.1"
                     /translation="MKAIGKSCLQLLVSLKRDAMLLAACLAPVLFGLLFRFGIPVLEG
                     YLTFRFKQADMLSPYYGLFDLLFSLLAPAMLCFAAAMVILEETDDHIAGYFYVTPLGY
                     RGFLASRVLIPSAAAFILTLLLLPLFVLTPLSAGSMLLMVLCGSMQGVLTTMLVVSFS
                     SNKLEGVAVTKISSLLMLGIFAPYFIKNSWQYLFSALPSYWIAKAVFEKQQLFFIPAV
                     FLSGIWYLCLAERFRKKTVK"
     gene            complement(2203..2886)
                     /locus_tag="PLU43_00380"
     CDS             complement(2203..2886)
                     /locus_tag="PLU43_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO26891.1"
                     /translation="MRWLHILKLDMKFQLKYGFYLVYFIVTVLYVALLAVIPKEYRET
                     TAVALIFSDPAAMGLFFMGAVLLLEKSQRVLNMLAASPVRLAEYMLSKIVSFVIFAEV
                     VAVILALAAGNDHIFMVMAGTAFSSVIFTLIGMIAAAGTNSLNQFILLTIPIEIICFV
                     PALLYLFGIRTNGLRFYPLNFCMSMLMGETGMLPVKLFMIAALIAVLSAAAYRRMNRM
                     FKSMGGMKL"
     gene            complement(2883..3728)
                     /locus_tag="PLU43_00385"
     CDS             complement(2883..3728)
                     /locus_tag="PLU43_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO26892.1"
                     /translation="MITVEHLYFSYTKLPFIENMNFSVEKGEIFGFLGPSGAGKSTLQ
                     KILIGMNTGYQGSAKLNGTECAGYTKSFYETIGVDFEFSTMYEKLTARENLMFFSSLY
                     QKKPRSILELMKAVALENDMDKRVSDYSKGMKARLNFVKAILHDPQILFLDEPTSGLD
                     PTNSRMMKDLILKEKNAGKTIILTTHNMQDATELCDRVAFIVNGEICALDSPHNLIMS
                     KGAATVNYTYEENGEQSGSCPIDRIREDKLLGELIAGNRLLSIHSSEPTLNDIFLDVT
                     GRTLI"
     gene            complement(3876..6323)
                     /locus_tag="PLU43_00390"
     CDS             complement(3876..6323)
                     /locus_tag="PLU43_00390"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOO26893.1"
                     /translation="MFSKESFKKDVKDNIKVLYRKTLEEANEQQIFQAVCYTVKDSII
                     DNWLETQEAYEKEDPKTVYYLSMEFLMGRALGNSLINLKFYKEVAQALNEIGVNLDGI
                     EDQEPDAALGNGGLGRLAACFLDSLATLGYSAYGCGIRYRYGMFKQEIRDGYQVEVPD
                     NWLKDGNPFELRRSEYAKVVKFGGNVRVDVDENGESHFSQENYQSVKAIPYDLPIVGY
                     GNGIVNTLRIWDAQPVECFQLDSFDKGDYRKAVEQENLARNICEVLYPNDNHYAGKEL
                     RLKQQYFFISASIQEAVEKYMRNHRDVHKLYEKVVFQLNDTHPTVTVAELMRILMDDY
                     GLGWDEAWAVTTRTCAYTNHTIMSEALEKWPIELFSRLLPRIYQIVEEINRRFIRQIE
                     AKYPGNQEKIRKMAIIYDGQVKMAHLAICAGFSVNGVARLHTEILEKQELKDFYEMMP
                     EKFNNKTNGITQRRFLLHGNPLLADWVTSRVGDDWITDLPKLSKLKVFAEDARAQQEF
                     MNIKYQNKVRLAKYILEHNGIEVDPRSIFDVQVKRLHEYKRQLLNILHIMYLYNEIKE
                     HPDMGFFPRTFIFGAKAAAGYKNAKLTIKLINTVADVVNHDASIGGKIKVVFIEDYKV
                     SNAEWIFAAADVSEQISTASKEASGTSNMKFMLNGALTLGTMDGANVEIVEEVGAENA
                     FIFGLSSEEVINYEKNGGYYPVDIFNNDADIRKVLMQLINGFYMPDDPEMFRDLYNSL
                     LNTQCTAKADTYFILKDFKPYAEAQKRVEAAYKDEKNWARMAILNVACSGKFSSDRTI
                     REYVNDIWHLNTVVLKK"
     gene            complement(6357..>6846)
                     /locus_tag="PLU43_00395"
     CDS             complement(6357..>6846)
                     /locus_tag="PLU43_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOO26894.1"
                     /translation="KYGKLLGGIRGKLESLDGNSAMDELNAAGELKFDIGGEAVVLSK
                     EDLLIEMVQKEGYVSENDNQMTVVLDTNLTPELIEEGYVRELISKLQTMRKEAGFEVM
                     DQIVVYAKDNEKIAGLFAAYKEEIMSEVLAVDIVTGSAEGYVKEWNINGEQVVLGVKR
                     NQ"
BASE COUNT         1893 a         1773 c         1264 g         1916 t
ORIGIN      
        1 tcttggcact gcagagaaag cgacggacat tttactttcc gcgtttgcca aaagtgcggc
       61 agcacatgac tggacactga aactaatcgg tccggtgaaa gaaacattca gaccgtctat
      121 tgacctgttc tgcgaacaat atccgcagct taaagaacgg gtaatatttt tggggaaaat
      181 aaccgataaa gaaacactga gtaaggaata cagaaaggca aagatcttta cacttccttc
      241 cagaagtgaa agttttggta tcgtacttgt tgaagcggca aacgaaggct gctatctcat
      301 tacgacagat atggtacctg ccggttatga tgtgaatgga aacggacgtt tcggactggt
      361 cgttccggca gataatgcgg acgctctggc aaatgcattt tcagacctgt gcggaaaaaa
      421 caattcccgc gactggaata aagaagcaca ggaaatctct ttatttgccc aaaaaacctt
      481 ctgctgggac acgatcatca gacagcttta caaagcaatt acggaatgtc cataaaaact
      541 catcccggat tttccatttc ccagacagga atatgaaaag aagcgttttg cctgcgaacg
      601 atgatctgca gcctgcggat cgcttttttt atccgttttg gaaaatagtc caccacaaac
      661 tgtttgatct taagttccgc agccttcttt ttcatctgcc gctcatcata ccggtgaata
      721 ccgtgtcctt cccatatatg gacagtttcc ataaacgtat catataactt catggaagag
      781 actccgtctt ttgaaacaat gcagacgata ttgtttgtat cggtaaagaa aaatcctttt
      841 tgatagactg tcagcagaaa atcataatcc gcaccgatcc gatagtccgt acaaaacgga
      901 agcctggcaa gaagctcttt tcttacaaac gctgactgat gagaaaaagg catccgcgac
      961 tcaatgctgt caaatgcctt tcggaacaga taatatctgc cgtattcaca ttccacggca
     1021 tctccgtata aaacagcgga attcgcataa tcacgttctt caaaaatatc ggaaagcaca
     1081 gactcgctgt agaaacagtc tcccgcattc ataaaattga tccatgtacc ggccgcatgc
     1141 tgcagcgctt tgttcatcgc atcataaata ccgccgtcct tttccatgga acagcggagt
     1201 gttattcctt tttccaaaaa acgcaaacga tagttctcaa gtaattctgc cgtaccgtct
     1261 gcagagcctc cgtcctgaat aatatactca aatccggcat atgtttgcgc cagaatagat
     1321 tccattgttt taatcagtga ttttccggga ttataacaga cagttatgac agatacgacc
     1381 ggaacattac ttccttcccg catgacagac tccttttgtg tctcctatca ttttctgtaa
     1441 ctttatccag tataacacga atcacctgat tattcaccgc cgaattttat tattttactg
     1501 tttttttccg aaaccgctct gccagacaga gataccagat gcctgacagg aacaccgccg
     1561 gaataaagaa caattgctgt ttttcaaaca ccgccttcgc gatccagtaa gacggcaacg
     1621 cggaaaataa atactgccag ctattcttta taaaataggg tgcaaaaatg ccgagcatca
     1681 gcagcgaaga aatttttgta acagctaccc cctccagctt gttggacgaa aaagaaacta
     1741 ccagcattgt tgtcagaacg ccctgcatgc tcccgcacag caccatcagc agcatggatc
     1801 cggcagaaag cggtgtcagc acaaataagg ggaggagcag caaagtcagt ataaatgccg
     1861 cagccgatgg aatgagtacg cgtgaagcaa gaaagccccg gtatccgagc ggtgtcacat
     1921 aaaaatatcc cgcaatatga tcatccgttt cctccagtat caccattgcc gccgcaaaac
     1981 aaagcattgc cggtgccaaa agggaaaaaa gcagatcaaa cagaccataa tagggagata
     2041 gcatatcagc ctgcttaaat ctgaacgtca aatatccttc cagtacggga atcccgaaac
     2101 gaaacagaag accgaacaat acgggagcaa ggcatgcggc cagaagcatc gcatcccgtt
     2161 tcaatgaaac aagcagctgc agacagcttt ttcctatcgc tttcataatt tcattcctcc
     2221 catgctttta aacattctgt tcattcttct gtaagcagcc gcagacagca ctgcaatcag
     2281 ggcagcgatc atgaacagct tcaccggcag catccctgtc tctcccatga gcatgctcat
     2341 acagaaattg agcggataaa aacggagtcc atttgtacgg atgccaaaca aatacaataa
     2401 cgccggaaca aaacatatga tttctatcgg gatcgtaagc agtataaact ggttcagact
     2461 gttggttccg gctgctgcga tcattccgat cagcgtaaat attacggaag aaaacgccgt
     2521 tccggccatg accataaaaa tatgatcatt tccggcggcg agcgcaagaa tcacggcaac
     2581 gacttccgca aaaatcacaa atgaaactat tttcgacagc atatattccg caagccttac
     2641 cggtgatgcc gcaagcatat tcaatactct ctggcttttt tcaagcaaaa gcaccgcccc
     2701 cataaaaaac agtcccatcg ccgccgggtc cgaaaaaatc aaagccacag ccgttgtttc
     2761 cctgtattcc tttggaatta cggcaagcag ggcaacatag agcaccgtca ctatgaaata
     2821 aaccaaataa aaaccatact ttaactggaa cttcatatcc agtttcaata tatgcagcca
     2881 tctcatatca gcgtcctccc cgtaacatcc agaaaaatat cgttcagggt cggctcactg
     2941 ctgtgaatgg aaagcagccg gttgccggca atcagctccc ctagcagttt atcctccctg
     3001 atccggtcga tcggacagct tcctgactgt tccccgtttt cctcataagt atagtttacg
     3061 gtcgcggcac cctttgacat gatcagattg tgcggactat ccaatgcaca tatttctccg
     3121 tttacaataa aagcgaccct gtcgcaaagc tccgttgcat cctgcatgtt gtgtgttgtc
     3181 agaataatgg tcttgcctgc atttttctcc tttaagatca gatccttcat catccggctg
     3241 tttgtcggat caagtccgct tgtcggttca tccagaaaca ggatctgcgg atcatgcaga
     3301 atcgccttta caaaattcag tctggctttc atgcctttcg aataatcgga aacccgctta
     3361 tccatatcgt tttccaaagc aaccgccttc atcagttcca aaatggaacg cggtttcttt
     3421 tgataaagcg atgaaaaaaa catgagattt tctcttgccg tcagtttttc atacattgtt
     3481 gaaaactcaa aatccacacc gatcgtttca taaaaacttt ttgtatagcc ggcacattcc
     3541 gttccgttca gtttcgccga gccctgatag ccggtattca tcccgatcag tatcttctgc
     3601 agcgtagatt tcccggctcc ggacggaccg agaaacccaa atatttcacc tttttccacc
     3661 gaaaaattca tgttttcgat aaatggaagt tttgtgtagc tgaaatacaa atgctctacc
     3721 gtaatcattt tatttccttt ctttttgact atattgacca tattattatt tttagtcatt
     3781 ataacgccag aaatgaaaac tgtcaatctt tttctttaaa atataagagc ggaaagcatt
     3841 gcgctccccg ctcagtctaa actataattg tcctgttatt ttttcagcac gaccgtattc
     3901 aaatgccaga tgtcattgac atattcccga atcgtcctgt cggacgaaaa ctttccggaa
     3961 caggcaacat tcaggatcgc catacgtgcc cagttctttt catctttata tgcagcttcc
     4021 acacgcttct gcgcctctgc atacggtttg aaatctttca ggataaagta cgtatccgcc
     4081 tttgccgtac attgtgtatt cagcagcgaa ttatacagat ctctgaacat ctccggatca
     4141 tcaggcatat aaaagccgtt gatcagctgc atcagtactt tgcggatatc tgcgtcgtta
     4201 ttaaaaatat ccacaggata atatccgccg ttcttttcat aattgattac ttcctcagag
     4261 gaaagaccga aaataaaagc gttttctgct ccgacttcct ccacgatctc cacattggct
     4321 ccgtccatgg taccaagcgt cagcgccccg ttcagcataa acttcatatt gctggtcccg
     4381 gaagcttcct tgcttgcggt cgatatctgt tcggacacat cggcagccgc aaagatccat
     4441 tccgcatttg atactttata atcctcaata aagaccactt tgatctttcc gcctatggaa
     4501 gcatcatgat tgacaacatc cgccaccgtg ttgatcagct tgatcgtcag ctttgcattt
     4561 ttatagcctg ctgccgcttt tgcaccgaaa ataaatgttc ttgggaaaaa ccccatatcc
     4621 ggatgctctt tgatctcatt atacagatac ataatatgaa gaatatttaa aagctgtctc
     4681 ttatattcgt gcagacgctt tacctgcaca tcaaatatgg aacgcggatc cacctcgatc
     4741 ccattatgct ccagaatata ttttgccagg cggaccttgt tctgatactt gatattcata
     4801 aattcctgct gtgctctggc atcctcggca aataccttca gcttgctaag ctttggaaga
     4861 tctgtgatcc agtcatcgcc gactctagat gttacccagt ctgccagaag cggattccca
     4921 tgcagtaaaa atcttctctg tgtaataccg tttgttttat tattgaactt ctccggcatc
     4981 atctcataaa aatcctttaa ttcctgcttt tccaaaatct cggtatgcag ccttgccaca
     5041 ccatttaccg aaaaaccggc acagatcgca agatgagcca tcttgacctg tccgtcatat
     5101 ataattgcca ttttacggat cttttcctga ttacctggat actttgcttc tatctgtctg
     5161 ataaaacgcc tgttgatctc ctccacgatc tgataaatac gcggaagaag tctggaaaac
     5221 agctcgatcg gccatttttc aagtgcctcc gacatgatcg tatgatttgt atacgcacag
     5281 gtccgcgtcg taacagccca cgcttcgtcc cagccaagtc cgtagtcatc catcaaaata
     5341 cgcatcaatt ctgcgaccgt aacagtcgga tgtgtatcat tcagctggaa aacaactttt
     5401 tcatacagct tatgaacatc cctgtggtta cgcatgtatt tttccacagc ctcctgaata
     5461 ctggccgaaa taaagaaata ctgctgcttt aagcgaagct ccttgcctgc ataatgatta
     5521 tcattcggat aaagcacctc acagatattt cttgcaagat tttcctgctc aactgccttt
     5581 ctgtaatctc ccttatcaaa agaatccagc tgaaagcatt cgaccggctg tgcatcccag
     5641 atccgcagtg tattgacgat tccgtttcca tagccgacga tcggcaaatc gtaagggatc
     5701 gcttttacgg actgataatt ttcctgtgaa aaatggcttt ccccgttttc atccacatct
     5761 acacggacat ttccgccgaa ttttacgacc tttgcatact cagaacgtct aagctcaaaa
     5821 ggattgccgt cctttaacca gttatccggc acttcgacct gatacccgtc acggatctcc
     5881 tgcttaaaca tcccgtagcg gtatctgatc ccgcagccat atgcactgta gccaagcgtt
     5941 gccagcgaat ccagaaagca ggccgcaagt ctgccgagtc cgccatttcc aagcgctgcg
     6001 tccggctcct gatcttctat cccgtcaaga ttaacaccga tttcgttaag cgcctgtgcc
     6061 acttccttat aaaacttcag attgatcaaa ctgtttccaa gcgctcttcc catcaaaaat
     6121 tccatggaca gataatatac tgtctttggg tcttcctttt cataagcctc ctgtgtttcc
     6181 agccagttat caataataga atcctttacc gtataacaca ctgcctgaaa aatctgctgc
     6241 tcatttgctt cttcaagcgt ttttctgtac aaaaccttga tgttgtcttt cacgtctttc
     6301 ttgaatgatt ccttgctgaa catacgtttc ttaaccaccg gcaaatcctc cttgtcctac
     6361 tggttccttt tcacacccaa tacaacctgc tctccattga tattccactc ttttacataa
     6421 ccttctgcag aaccggtcac aatatcaacg gcaagtacct ccgacatgat ctcttcttta
     6481 tatgctgcaa aaagacctgc gatcttttca ttgtcttttg catatacgac gatctgatcc
     6541 atcacttcaa accctgcttc cttccgcata gtctgaagct tgctgatcaa ttccctgaca
     6601 tagccttcct cgatcagctc cggcgtcaga ttcgtatcaa gcaccactgt catctgatta
     6661 tcattctcag atacgtaacc ctctttctga accatttcta ttagcaaatc ctcttttgat
     6721 aatactacgg cttcaccgcc tatgtcaaat ttcagctcgc ctgcggcgtt cagctcatcc
     6781 atggcggaat ttccgtccag tgattccaat tttccccgaa taccgccgag cagtttccca
     6841 tatttg
//
LOCUS       DAOSYE010000011         6715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7423_length_6715_cov_3.096547, whole genome shotgun sequence.
ACCESSION   DAOSYE010000011 DAOSYE010000000
VERSION     DAOSYE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7423_length_6715_cov_3.096547"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLU43_00400"
     CDS             complement(<1..430)
                     /locus_tag="PLU43_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26895.1"
                     /translation="MNVMIVYDSYFGNNEKAARIMSDAITLAHQVKAVHIKEMQKRLP
                     KETDLLVIGTPTRVFRPSGPVIRFIKSLDRDFLKDRMVSVYDTRMDVGKLDVPILKVL
                     HTCRGSAAAYMEKKLLAKKAVIIGEPAGFVVCDSVGPFEEG"
     gene            complement(445..1221)
                     /locus_tag="PLU43_00405"
                     /pseudo
     CDS             complement(445..1221)
                     /locus_tag="PLU43_00405"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters.; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
     gene            complement(1422..2267)
                     /locus_tag="PLU43_00410"
     CDS             complement(1422..2267)
                     /locus_tag="PLU43_00410"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO26896.1"
                     /translation="MQLTEIKKIFLFIGIGTILLIIFLIYHAVYHMILKPMEQLEYKL
                     AEFSDSGDFPEISEAVYQRQDEIGRIAKSFGNMKKRLCESREETEQYKKELVEIILKT
                     LHEKSPREEQHSRRVAELSERLAAAMGKSKTECRRIYHAGMLHDIGKIGISEEILNKP
                     GRLDDEEYQLIRRHPEIGYCILQVSGETKDLSEIVLYHHERYDGRGYPKNLKRDEIPL
                     EARIISVVDSFDAMTSNRSYRRGLSTEEAVRELLSNAGTQFDSDIVDIFVERVVKYKA
                     IQKVI"
     gene            complement(2273..2797)
                     /locus_tag="PLU43_00415"
     CDS             complement(2273..2797)
                     /locus_tag="PLU43_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO26897.1"
                     /translation="MGPYVYRQDDRIVTTYEYADKKYDYFSQDFYRLPLESKDTVFTD
                     VYYDEALDKYIITCSSPIWGNDRAYLGCTTIDMELDSLETLINNYSGKHIGEMYIISN
                     DGQYLADCDSELVKSGGNIMETACESFQPLVKELMMNDSGQGTYTDNGEKVFVYYDTL
                     AEFGWKTVYEIPEN"
     gene            complement(2846..3148)
                     /locus_tag="PLU43_00420"
     CDS             complement(2846..3148)
                     /locus_tag="PLU43_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26898.1"
                     /translation="MSRHIRTKLILILLPVVIGTTVFMYFFSINYTKRIIMEKTQEQV
                     NADYKAENALISKVLDQSEQIANSLSIYIGETYQKSSLSDYEKIMSAVLTENNTLL"
     gene            complement(3224..5224)
                     /locus_tag="PLU43_00425"
     CDS             complement(3224..5224)
                     /locus_tag="PLU43_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO26899.1"
                     /translation="MKSIKTKIMVGFCVLLLLVCMGLGFSSYFFASEALTKSAGEQLE
                     DIAKQGATVVCRALDEQWSSLEVLAENDVICDPDSSWEEKTEILQKEVERSGAVNVMF
                     ADVQGNTKSLDGEDVSISEREYFQKALEGEFAVSDPIEDKTNPGSMIMTFAVPVKSND
                     QIIGVLVKAASGTMLSEITDSITFGESGQAYMVNGEGTLVANSNRDLVLQMDNVFQDA
                     QEDSSYDKMAEAVQKMITGESGNSHYIYGGVQKYIGYYPVEGTDWFLAVTAPKDDILS
                     GLGTLKISTVSVSTVFMIIGIIVSLILATLIAKPITLLTKKLSVISGGDFSQAVDTKL
                     IGIKDETGMLAKEIEKMRNSVKDVVKAVMQESQDVFDNVTNQEGKVSQLLSQIEDVSA
                     TTEQLSAGMEETAASSEEMNTAADEIEKAVESIAVRAQEGAQTVNEIYERAKEYKENA
                     IKAKGMAQDIYQSSSEELREAIRQSKDVEQINALSEAVLQITEQTNLLALNAAIEAAR
                     AGEAGKGFAVVADEIGRLAESSKSSVAEIQRVTKSVVTSVENLSQNSVQVLDFIKDKV
                     MNDYESLVQISEKYHDDADTVDSLVTDLSATTEELTSSINNIMNSINGITTATNEGAE
                     GTTSIAQNVVIVGQAAKEVVEYASDTKDSGNKLINAVAVFKI"
     gene            complement(5388..6098)
                     /locus_tag="PLU43_00430"
     CDS             complement(5388..6098)
                     /locus_tag="PLU43_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HOO26900.1"
                     /translation="MYEGCRRIVDMHDGMMERLSSVRSQEEVLKIGATDIIYSERFSK
                     NIVNFHESNPNIQMEFVSLGYYDCERYLENDIVDLCITMKLDSLAKYKFKRVWTDLFH
                     LYTDKANPLASKTKVRIKDLANEKIILLSNNTKGGSMLREYFIDKGFIANIILSTSQM
                     SLAFEYVTAQMGVAILPFYGTPQEYCRRKDVVSIEIQDLDCFCEMGILYLKDKMLSRQ
                     ESDFISYFADTVNCWDDL"
     gene            complement(6071..6286)
                     /locus_tag="PLU43_00435"
     CDS             complement(6071..6286)
                     /locus_tag="PLU43_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO26901.1"
                     /translation="MDIMQMRCYMQLYEDRSFTKAASRMFISQQGLSRIIKSMEDEYQ
                     VSFFHRTSEEWSRRRKEILCMKAAEEL"
BASE COUNT         1746 a         1740 c         1073 g         2156 t
ORIGIN      
        1 ctccttcttc aaaaggtcct accgagtcgc agacaacaaa gcccgcgggt tctccgataa
       61 taaccgcctt tttagccaac agcttctttt ccatataggc agctgcactt cccctgcagg
      121 tatgcagtac tttcagtatc ggcacatcca gtttaccaac atccatcctt gtatcataca
      181 cagaaaccat gcggtctttt aaaaaatctc tatccaacga ttttatgaat cggatgacag
      241 gccctgaagg ccggaacact ctcgtcggcg ttccgatcac gagcagatcc gtttcctttg
      301 gaagtctttt ttgcatctcc ttaatatgca ccgccttcac ctggtgagca agcgttattg
      361 catcgctcat tatcctggct gctttttcat tgtttccgaa ataagaatca tatacgatca
      421 ttacattcat gttggttctc ctctctactt tgtctcatcc tccagataag gaggcagttt
      481 cgtgtctttg ataatttcct caaggctgta tccttccttc aatatgcaga atcccatata
      541 accggattcg atcgcacgga ccgggcatcc gtagatacat ctcatgcacc agatacactc
      601 ttttccaaat accggccggt cagcagcaac acggatgttt tttgcaggac agctttggac
      661 gcatttgaaa catccgatac agcggctgtc cgctgttaat gactttccga aaaatttcgc
      721 accggttccg gttccaagac ggccgactcc tgttgataac aactgctgaa gaccgttact
      781 tttatacaat ctccttgttc cattcagaac ttcccctgcc atatgcgctg catttttttc
      841 agcggaaata tacagctgtt ttgccagctg tttgtcatat gcaaaaagcc agttagaacc
      901 tataacaaag agccggtcat aaaagacatc atatcctttc tttctcagtt ttctgatcag
      961 accatctgat gccgcatagt tcagtacgga atcatcaccc gctgttttaa atataaatac
     1021 tttcttttta agcgaaggca gtgtatccgc ccagacccat acatttttcg gtgtcgaaaa
     1081 cgagtacacc ggatgaccga ttccgatcag atcatacccg ctaagatcca tctgccggct
     1141 ttccgatgat tccatacttt ttacggtaac aaaaaaaccg ctccttgtaa aatgctctga
     1201 aaggaatcct gctgccgctt ttgtatttcc ggttccttaa aaataaaaaa taatcacctg
     1261 cttcattttc tgcctgcttt cctttagtac aatattctat attttataac actgttatat
     1321 tatagactac atccatcctt ttgtctacag gtatcaatac attatttaag ccccaatgca
     1381 agcgcagcta tgctgctcac attggggcct gttttatcat cttaaatgac tttttgtatt
     1441 gctttatatt ttacgactct ttccacaaag atatccacga tatcggaatc aaactgtgtg
     1501 cccgcattac taagcagctc tctgacggct tcttccgtgc tcagtcccct gcggtaactc
     1561 cggttgcttg tcatggcatc aaaagaatcc acgactgaaa taattcttgc ttcgagcggg
     1621 atctcatccc ttttcaaatt tttcggatac cctcttccgt cataccgttc atgatggtag
     1681 agcacaattt cagacagatc ttttgtctct cccgacactt gcaaaataca ataaccgatc
     1741 tccgggtgtc tgcgtatcag ctgatattct tcatcatcaa gccttccggg cttgttcaga
     1801 atttcctctg aaataccgat tttccctata tcatgcagca tgcccgcatg atagatccgc
     1861 ctgcactctg ttttgctctt tcccattgcc gcagccagcc tttcgctcaa ctcagccact
     1921 cttctggaat gctgctcttc ccttggactt ttttcatgca gggtctttag aatgatctca
     1981 acaagttctt ttttatactg ctctgtttct tcccttgact cacacagtcg tttcttcata
     2041 tttccaaagg acttggcaat tctgccgatc tcatcctgtc tttgatatac tgcctcagaa
     2101 atttccggga aatcgccgga atcactgaat tcggccagtt tatattccag ctgctccatc
     2161 ggttttaaga tcatatgata aacagcatga taaatcagaa aaataatcag cagaatcgta
     2221 ccgatcccga taaacagaaa gattttcttt atttccgtca attgcaccgt gatcagtttt
     2281 ccggaatttc ataaacggtt ttccatccaa actccgccag cgtatcataa tatacaaata
     2341 ctttttctcc gttatcggta tatgttccct gtccggaatc attcatcatc aattctttta
     2401 caagcggctg aaagctttcg caggctgttt ccattatatt tccgccgctt ttaacaagct
     2461 cagaatcgca atctgcaaga tactgcccgt cattgctgat aatatacatt tccccgatat
     2521 gcttcccgct gtaattattg atcagcgttt ccagcgaatc cagctccata tctatcgtcg
     2581 tacagccaag atacgctcga tcgttccccc agatcggact gctgcaggtt attatatact
     2641 tatccagtgc ctcatcataa taaacgtctg taaataccgt gtccttgctt tccagcggaa
     2701 gcctgtaaaa atcctgcgaa aaataatcat attttttgtc cgcatattca taggtagtca
     2761 cgattctgtc atcctgcctg taaacataag gccccacata ttttctggaa gtgtcaaata
     2821 cattcggctc aaaccatact ccgattcaaa gcagcgtatt attttccgtc aatacggcgc
     2881 tcatgatttt ttcataatcg cttaaactgc ttttctgata cgtttctcca atatagatcg
     2941 acaatgagtt tgctatttgt tcactctgat ccaaaacctt tgaaatcaat gcgttttcag
     3001 ccttataatc cgcattcacc tgctcctgtg tcttttccat aatgattctt tttgtataat
     3061 taatactgaa aaaatacata aatacagtcg ttccgattac cactggtaaa agaatcaata
     3121 ttagtttcgt tctaatatgc ctgctcattt ttcctctcta ctccggtcac aaccttccag
     3181 cgcttatcaa tgtatgagga tagaaccttt tatataaacg atatcagatc ttgaacacag
     3241 cgactgcatt tatcagttta ttcccgctgt ccttcgtatc tgacgcatat tccaccactt
     3301 ctttcgccgc ctggccaact atcactacat tttgcgcaat actcgttgtt ccttctgccc
     3361 cttcattagt tgctgttgta atgccgttta tagaattcat gatattgttg atcgaagaag
     3421 tcaattcctc tgtcgtcgca cttaagtctg tcaccagact gtcgaccgta tccgcatcat
     3481 catgatattt ttcactgatc tgtacaaggc tctcataatc attcatgacc ttatctttta
     3541 taaagtccag aacctgaaca gaattttggg aaagattttc caccgaagtc acaacactct
     3601 ttgtcacccg ctggatctca gccacagagc tcttagagct ttcagcaagt cttccgatct
     3661 catcggcaac gacagcaaat ccctttcctg cctctcctgc ccttgccgct tcgatcgccg
     3721 cattcagggc gagaagattt gtctgctccg tgatctgcag tacggcctcg gacagtgcat
     3781 tgatctgttc cacatccttt gactgcctga tcgcttcccg cagctcttct gaagaagact
     3841 ggtaaatatc ctgtgccatt ccctttgctt ttattgcatt ctctttatat tcctttgccc
     3901 gctcgtaaat ctcgtttaca gtctgggctc cttcctgcgc cctgaccgcg atcgattcca
     3961 ctgccttttc gatctcatct gccgcagtat tcatttcctc agaggacgcc gctgtttctt
     4021 ccatccctgc cgacagctgc tccgttgtag cggaaacatc ctctatctgt gataagagct
     4081 gcgatacttt cccttcctga ttcgtaacat tatcaaaaac atcctgtgac tcctgcataa
     4141 cagctttgac cacgtctttt acagaattcc gcattttttc aatttctttt gcaagcatgc
     4201 cggtttcatc cttgattccg atcaatttcg tgtccactgc ctgtgaaaag tctcctccgg
     4261 atattacact gagttttttt gtcagcagtg tgatcggttt tgcgatcagt gttgccaaaa
     4321 tcagactgac aatgataccg atgatcataa acacggtcga taccgatact gttgatatct
     4381 tcagcgtacc aaggcctgaa agaatatcgt ccttcggtgc tgtgaccgcc agaaaccagt
     4441 cggttccttc aacaggataa taaccgatat atttttggac gccgccgtat atataatggc
     4501 tgtttccgga ctctcctgta atcattttct gcactgcttc cgccattttg tcataactgg
     4561 aatcctcctg tgcatcctga aaaacattat ccatctgcaa taccagatcc ctgttactat
     4621 tagcaaccaa ggtgccttcg ccgtttacca tatatgcctg tcccgattcc ccaaaggtaa
     4681 tgctgtccgt aatctcactc agcattgtac cgctcgcggc cttaacaagc acgcctatga
     4741 tctgatcgtt gctttttacc ggcactgcaa atgtcatgat catggaaccc ggatttgttt
     4801 tatcctcaat cgggtccgac acggcaaatt ccccctccag tgccttttga aaatactccc
     4861 tctcactgat cgatacatct tctccgtcaa gtgactttgt attgccctgc acatccgcaa
     4921 acattacatt gacggcacca cttctttcaa cttccttctg taaaatttcc gttttttcct
     4981 cccaggaact gtccggatca cagatcacat cattttctgc caatacttcg aggctgctcc
     5041 actgctcatc aagcgctctg catactacgg tagcaccctg ttttgcgata tcctccagct
     5101 gctcacctgc cgactttgtc aatgcctcag acgcaaaaaa atatgaggaa aagcctaacc
     5161 ccatacagac aagtaacagt aacacacaaa atccgaccat aatttttgtt ttaatgcttt
     5221 tcatattttg aacccctctc tactttaaat aacgacaaat ttatgaattg aatcgcagaa
     5281 tagcagaata aacagaacag tatgctacct catcacaaga taggataaca cataatataa
     5341 tccaataaaa tcatcaattt gtttattttt tacaacaaat tgttgtttta aagatcatcc
     5401 cagcagttta cagtatcggc aaaataacta ataaaatcac tttcctgcct ggaaagcatc
     5461 ttatctttta gatacaagat ccccatttcg caaaaacaat ccaaatcctg gatctctata
     5521 cttactacat cctttctcct gcagtattcc tgcggagtcc cataaaacgg caggatcgcc
     5581 acacccatct gcgctgtcac atattcaaag gcaagggaca tctgcgatgt tgacaaaata
     5641 atatttgcga taaatccctt gtcgataaaa tattcccgca gcatggatcc gccttttgtg
     5701 ttatttgaca acaaaatgat tttttcattt gccaggtctt tgattctcac ctttgttttt
     5761 gacgccaaag gattcgcctt atccgtatac agatgaaaca gatccgtcca gaccctttta
     5821 aacttatatt ttgcaaggct gtccagtttc atcgtaatac acagatcaac gatatcgttt
     5881 tccagatatc gctcacagtc ataatatccg agcgaaacaa attccatctg tatatttgga
     5941 ttgctttcat gaaaattaac gatatttttt gaaaaacgtt cggaataaat aatatccgta
     6001 gctccgatct taagaacctc ttcctggctc cgcacactgg aaagccgttc catcattcca
     6061 tcatgcatgt ctacaattct tctgcagcct tcatacataa tatttccttc cggcgtcggc
     6121 tccactcttc gcttgttcta tgaaaaaaag aaacctgata ttcatcctcc attgatttaa
     6181 tgatcctgct cagcccctgt tgtgagataa acattctgga tgcagccttt gtaaaggatc
     6241 tgtcctcata tagctgcata tagcatctca tctgcatgat atccataaat aaacgacctc
     6301 caactattat tttttctgac attttgagag ttttatattc aaaataatac cctactcaaa
     6361 tctttcttct attctcatta aaataacata ttggcacgtt tattcccttt gtaataattt
     6421 gttccgagac ttttgagcag ggcatataaa gagttagaga ctaacccagc ctgaattagt
     6481 ctccatatcc atactcccct ctacttcttc tgtcatacaa ttattattac cctaaacaac
     6541 ctgttttcac tttctgaatc gatcctgttt ctgaaattac atctccttcc taacgcagcc
     6601 atctgtatac atgctttatt atttatacaa aatatctaaa aattatatat atatctcatt
     6661 aaaatatcaa tgccatttag ttaggcttaa ttcaatttaa ttttagtggt tttcc
//
LOCUS       DAOSYE010000012         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7636_length_6584_cov_3.206310, whole genome shotgun sequence.
ACCESSION   DAOSYE010000012 DAOSYE010000000
VERSION     DAOSYE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7636_length_6584_cov_3.206310"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLU43_00440"
     CDS             <1..836
                     /locus_tag="PLU43_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOO26902.1"
                     /translation="GLCLVIGGALLYKFKKINLAAILAGCGIFSTFFGFMFGSFFGFE
                     DVIEPVWIRPVSYMATVPFIGKLNYIFIVTVGFGMFLILLLMIFNIINSIKEHDITKA
                     LMDINGVAGLVFFGSAVLVIILLMTGNHLPAGIVAAAMFGIPLILFILKEPISRFVEK
                     KAKLLPDGKGMFVVQTIFELFEVLLSYFSNTLSFVRIGAFAVSHASMMEVVLMLAGAE
                     EGTPNWIVIIVGNLFVCGLEGLIVGIQVLRLEYYEMFSRFYRGTGREFKPYRMQENAL
                     K"
     gene            855..1286
                     /locus_tag="PLU43_00445"
     CDS             855..1286
                     /locus_tag="PLU43_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOO26903.1"
                     /translation="MTETVVKVLLAAALILSIVIPFGAFLIGEHTRGRYKTTIGINAF
                     FFFSAMLIANIFMYSNVSAASTVSTSDLSNGLGYLAAALSTGASCIGGGIAVASAASS
                     ALGAISEDQSILGKSLIFVGLAEGIALYGLIISFMILGKLG"
     gene            1286..1594
                     /locus_tag="PLU43_00450"
     CDS             1286..1594
                     /locus_tag="PLU43_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOO26904.1"
                     /translation="MKMYLISDNIDTYTGMRLAGVEGAVVHERQELLDELNKAIADKE
                     IGIILLTEKFGKNFPDIIDDVRLHRKQPLLIEIPDRHGTGRAPDFITSYVSEAIGLKL
                     "
     gene            1640..2227
                     /locus_tag="PLU43_00455"
     CDS             1640..2227
                     /locus_tag="PLU43_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26905.1"
                     /translation="MTTDEKLQELLDTTMANARKQANEIIKGYNASADRILEDYRQSK
                     REYIESRISAECSALKTAENKEVSATQIVIRKELSYKQEELKGKLFSEIVVLLDNYKK
                     TEEYEKLLVSQIREEKNFAGENPLTVYIDPADAEKKDRLEKETGCVLTVSAYSFIGGT
                     RAVTNDNKILIDNSFQTALEEERDKFILQGGRTNE"
     gene            2220..3992
                     /locus_tag="PLU43_00460"
     CDS             2220..3992
                     /locus_tag="PLU43_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO26906.1"
                     /translation="MNDDRTGIIYGINGPVIYLKGNTGFKMGEMVYVGEEQLVGEVIG
                     LTTEQTTIQVYEETSGLKPGAIVKATGGAIFVTLGPGIINNIFDGIERPLKSIAEKSG
                     IYINRGINAASLDSEKKWDVKIEVKDGQQVEGGTIIAELQETTAIVHKSMVPPYVKGV
                     IKNPVPDGAYTVEDVIATVETEQGEEIPLRLMQKWPIRVPRPVSKRYSTHIPLITGQR
                     ILDTLFPIAKGGTAAIPGGFGTGKTMTQHQIAKWSDADIIIYIGCGERGNEMTQVLED
                     FSKLEDPKSGNKLMARTTLIANTSNMPVAAREASIYTGVTLAEYYRDMGYDVAIMADS
                     TSRWAEALRELSGRLEEMPAEEGFPAYLASKLSAFYERAGLVANLNNSEGSVTIIGAV
                     SPQGGDFSEPVTQNTKRFVCCFLGLDKSLAYARHFPAIHWLTSYSEYLPDLTPWYQEN
                     VSPDFMRYRSRIITILNQESNLLEIVKLIGSDVLPDDQKLILEIAKVIRLGFLQQNAF
                     HQEDTCVPMAKQYKMLDTILYLNDRSRELIAKGMPMSVLKESNIFERVISIKYDVPND
                     RLELFDQYKADIDAFYDEVLKKNQ"
     gene            4041..5426
                     /locus_tag="PLU43_00465"
     CDS             4041..5426
                     /locus_tag="PLU43_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOO26907.1"
                     /translation="MSIQYIGLNKINGPLVVLEGVKNAAYDEIVEITIDGKTNKIGRI
                     VEIYEDKAVIQVFEGTENMSLKNTHTKLTGHPMEFGLSTEILGRSFNGIGHPIDGLGE
                     ITVDEYRDINGLPLNPVSREYPRNYIRTGISAIDGLTTLIRGQKLPIFSGNGLPHDEL
                     AAQIVRQASLGEDSNEEFAIVFAAMGVKHDVAGYFKRVFEESGVSSHVCMFLNLANDP
                     VVERLLTPKIALTAAEYLAFEHDMHILVILTDMTSFAEAMREVSSSKGEIPSRKGYPG
                     YLYSELATIYERAGIASSAKGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDRSL
                     HGQGVYPPISILPSLSRLMKDGIGEGFTRADHQDVANQIYASYAKVQDARALASVIGE
                     DELSPIDKKYIAFGKEFESRFIGQGMQQNRTVIETLDLAWELLGMLPKEELDRIDTKI
                     LNEMYRPTALG"
     gene            5468..6091
                     /locus_tag="PLU43_00470"
     CDS             5468..6091
                     /locus_tag="PLU43_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOO26908.1"
                     /translation="MNVSTVPTKGNLMSLQNSLKLAKQGYELMDKKRNILITELTGLI
                     SEAKSIQSEIDMTFTKAYEALQVANIELGISYVQEIAKAMPVEDSIVIKTRSVMGTEI
                     PRVEMQNIDTKPTYAFYSTRYSLDKAREAFQKVKELTVRLSAVENSAYRLAANIRKTQ
                     KRANALNNITIPTYTALCKNIQAALEEKEREEFTRLKVIKRNKQKEA"
     gene            6215..>6584
                     /locus_tag="PLU43_00475"
     CDS             6215..>6584
                     /locus_tag="PLU43_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861708.1"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HOO26909.1"
                     /translation="MDSKTIQILTAMTIYMAAVIIIGIAFAKKANKSSENYFLGGRTL
                     GPWVSAMSAEASDMSGWLLMGLPGVAYWCGLADAAWTAIGLAIGTYINWLVVSKRLRR
                     YSIKVNAITLPEFFSNRYDED"
BASE COUNT         1955 a         1194 c         1690 g         1745 t
ORIGIN      
        1 agggactgtg tcttgtgatc ggaggcgcac ttttatataa attcaagaaa ataaatctgg
       61 cggcgattct tgccggctgc ggaatttttt ctacgttttt tggatttatg ttcggcagtt
      121 tctttggctt tgaagatgtg atcgagccgg tttggatcag accggtatcc tatatggcaa
      181 ccgtaccgtt tatcgggaaa ctgaattata tttttattgt tacggttggt ttcggcatgt
      241 tccttatttt gctgctaatg atatttaaca ttatcaattc gataaaagaa catgatatta
      301 caaaggccct gatggatata aacggggtgg cgggacttgt atttttcggc tcggcggttc
      361 ttgtaatcat actgcttatg acaggaaatc atcttcctgc cggaatcgtg gctgcggcga
      421 tgtttggcat cccgttgatt ctgtttatat taaaagagcc aatcagcaga tttgttgaaa
      481 aaaaggcaaa gcttttaccg gatggaaaag gaatgtttgt tgttcagacg atttttgaac
      541 tgtttgaagt gcttctgagt tatttttcca atacattgtc ctttgtgcgt atcggtgcgt
      601 tcgccgtcag ccatgcgtcc atgatggaag ttgtgctgat gcttgccggt gcggaggaag
      661 gaacgccgaa ctggatcgta attatagtag gcaatttgtt tgtctgtggt ctggaaggtc
      721 tgattgtcgg cattcaggta ttaaggcttg agtattatga gatgttttca aggttctatc
      781 gcgggacggg aagagaattc aaaccctata gaatgcagga aaatgcatta aaataaaaag
      841 aggaggtcat tcaaatgaca gagacagtag taaaagtttt attggcagcg gcattgattt
      901 tgagtatcgt aattcccttc ggtgcatttt tgatcggaga acacacacgg ggaagatata
      961 agacaacaat tggtatcaac gcatttttct ttttttccgc aatgcttatt gcaaatattt
     1021 ttatgtattc taatgtatcc gcagcaagta cggtatcgac atccgatctt tcaaacggac
     1081 ttggctatct tgcggcggcg ctttccaccg gagcatcctg tatcggcggc ggtattgccg
     1141 tagcctcggc agcaagctcg gcgctcggcg ccatcagcga ggatcagagc attcttggta
     1201 agtccctgat ttttgttggt ctggcagaag gaattgcact ttacggcctg attatttcct
     1261 ttatgatctt aggtaaatta ggctgatgaa aatgtatttg atcagcgata atatcgatac
     1321 ctataccgga atgcgtcttg ccggcgtgga aggggccgta gtccatgaaa ggcaggagct
     1381 tttggatgaa ttgaacaagg caattgcaga taaagaaata gggatcatat tattgacaga
     1441 aaaattcgga aaaaattttc cggacattat cgacgatgta cgtcttcaca ggaagcagcc
     1501 gctgttgatc gaaataccgg acagacatgg gaccggacgc gcaccggatt ttattacttc
     1561 ttatgtaagc gaagcaattg gactgaagct gtagcatgga ataaagcgtt caaaatttga
     1621 aggaaagaag gcgaatctta tgacaactga tgaaaagctg caggagcttt tggatacaac
     1681 gatggccaat gcaaggaaac aggccaatga aatcatcaaa ggctataatg cctctgccga
     1741 tcggatattg gaggactaca ggcagagtaa acgtgaatat atagagagcc gtatttccgc
     1801 agagtgctct gcgcttaaaa cagcggaaaa taaagaagta tccgcaacac agatcgttat
     1861 cagaaaagag ttatcctata aacaggaaga attaaagggt aagctgtttt cagaaatcgt
     1921 tgttttactg gacaattata agaaaaccga ggagtatgag aaattattgg tttcgcagat
     1981 ccgggaagag aaaaattttg ccggtgaaaa tccgctgacg gtttatattg atcctgcgga
     2041 tgcggagaaa aaggacagac ttgagaaaga aaccggctgt gtgctgactg tcagtgctta
     2101 ttcatttatc gggggaacaa gggctgtaac gaatgataat aaaattttga tagataattc
     2161 attccagaca gctttggagg aggagcgaga taagttcatc cttcagggag gcagaacaaa
     2221 tgaatgatga tagaacagga attatttatg gaataaacgg accggtcatt tatttaaagg
     2281 gaaatacagg ctttaaaatg ggtgaaatgg tctatgtagg agaagaacag ctcgtcggcg
     2341 aggtgatcgg actgactacg gaacagacga cgatccaggt ttatgaggaa acgagcggat
     2401 taaaaccggg agcaatcgtc aaggcgaccg gcggtgcgat ttttgtaacc ctgggtcctg
     2461 gtattatcaa taatattttt gatggtattg aacgtccgct aaaatcgatc gcggaaaaat
     2521 ccggtattta tatcaaccgg ggtattaatg ccgcatcact tgattctgaa aaaaaatggg
     2581 atgttaaaat agaagtgaaa gacggtcagc aggtagaggg cggcacgatc attgccgaac
     2641 tgcaggaaac aacagcgatc gttcataaat caatggttcc gccttatgta aaaggtgtta
     2701 ttaaaaatcc ggttccggac ggtgcgtata cggtcgagga tgtgatcgct acggtcgaga
     2761 ctgaacaagg agaagaaatt ccgctcagac tgatgcagaa atggccgatc cgtgtgccga
     2821 gaccggtcag caagcgttat tccacgcaca ttccgctgat tacgggacaa cggatccttg
     2881 atacactgtt tccgatcgca aaaggcggga cagcggcaat tccgggaggc ttcggaacag
     2941 gaaaaacgat gacgcagcac cagatcgcga aatggtcgga tgccgatatt attatttata
     3001 ttggatgcgg agagcgcgga aatgaaatga cacaggtgtt agaggacttt tccaagctgg
     3061 aggatccgaa atccggaaac aagctgatgg caagaacgac attgatcgcg aatacgtcga
     3121 acatgccggt ggcggccagg gaagcttcta tttatacagg tgttacgctg gcggaatatt
     3181 atcgcgacat gggatatgat gtggcaatta tggctgattc cacatccaga tgggctgaag
     3241 cgttaagaga gttatcagga cgtttggagg aaatgcctgc agaggaagga ttcccggcgt
     3301 atctggcttc taaactatct gccttttatg aaagagcggg tcttgttgca aacttaaata
     3361 attcagaagg aagcgtaacg atcattggcg cggtatcccc gcagggcggt gatttttccg
     3421 aaccggtcac gcagaatacg aaacgatttg tatgctgctt cttaggactt gataaatcgc
     3481 ttgcctatgc aagacatttt ccggctattc actggctgac aagctacagc gaatatctgc
     3541 cggatctgac gccttggtat caggaaaatg tgtcgccgga ttttatgcgt tatcggagca
     3601 ggattattac gattttgaat caggaaagca atctgcttga aatcgtaaag ctgatcggca
     3661 gcgatgttct gccggatgat cagaagctga ttcttgaaat tgcaaaagtg atcaggctcg
     3721 ggtttttaca gcaaaatgct ttccatcagg aagacacctg cgtaccaatg gccaagcagt
     3781 ataaaatgct tgatacgatc ctttatttaa atgatcggtc aagggagctg attgcaaaag
     3841 gtatgccaat gtcggtatta aaagaaagca acatttttga acgcgtgatt tctatcaagt
     3901 atgatgtgcc gaacgaccgg ctggaactgt tcgatcagta taaagcggat attgacgcgt
     3961 tttatgatga agtgcttaag aagaaccagt aaatcgtttc agaggagttt tatgaaagta
     4021 tccgaaaaga gggtgaatat atgtcgattc aatatatcgg attaaataaa ataaacggac
     4081 cgcttgttgt tctggaaggt gtaaaaaatg cagcctatga cgaaatcgtg gaaattacga
     4141 tagacggaaa gacaaacaaa atcggccgta ttgtggaaat ttatgaggac aaggcagtga
     4201 tccaggtatt tgaaggcacg gaaaatatgt cgctgaaaaa tacgcataca aagctgaccg
     4261 gacatccgat ggagtttgga ctgtcaacag aaatacttgg aagatcattt aatggaatcg
     4321 gacatccgat cgacggactt ggggaaatta cggtcgacga atacagggat attaacgggc
     4381 ttccgttaaa tccggtgtca agagaatatc cgagaaacta tattcgcacc ggtatatcgg
     4441 cgatcgacgg cctgacgacg ttgatccgag gtcagaagct gcctattttt tcgggaaacg
     4501 gacttccgca tgatgaactg gcagcacaga tcgtacggca ggcatctctt ggtgaggatt
     4561 ccaatgaaga gtttgcaatt gtatttgctg caatgggtgt gaagcatgat gttgccggct
     4621 atttcaaaag ggtatttgaa gaaagtggtg taagcagcca tgtatgcatg tttctgaatc
     4681 tggcaaatga tccggttgtc gagcgtctgc tgacgcctaa gatcgcactt acggctgccg
     4741 aatatctggc ctttgagcat gacatgcaca ttcttgttat tttgacggat atgacatcat
     4801 tcgcagaagc aatgcgggag gtttcgtcct ctaaaggaga gattccaagc agaaaagggt
     4861 atccgggata tttatacagt gaacttgcta cgatctatga acgtgccggc attgccagca
     4921 gtgcaaaagg ctctgttacg cagattccga ttctgacgat gccaaatgat gatattacgc
     4981 atccgatccc ggatcttacc ggatatatta cggaagggca gatcgttctt gaccgttctt
     5041 tacacggtca gggcgtttat ccgccgatca gtattttgcc gtcgctttcc cgtctgatga
     5101 aggatggtat cggggaaggg tttacaagag cggatcatca ggacgtggca aatcagatct
     5161 atgcttccta tgccaaagta caggatgcca gagcacttgc ttctgttatc ggagaagatg
     5221 aactgtcacc gatcgataaa aaatatattg ccttcggaaa agaattcgaa tcgagattca
     5281 ttggtcaggg catgcagcag aaccgtacgg tgatcgaaac gctggatctg gcatgggagc
     5341 ttcttggtat gctgccgaag gaagagcttg accgaatcga cacgaagatt ctgaatgaaa
     5401 tgtatcggcc aacagccttg ggataattta aagccatata tcatatatcc ggaaaggagg
     5461 gtcttttatg aatgtaagta cggttcctac caaaggtaat ttaatgtctt tgcagaattc
     5521 actgaagctt gcaaagcagg gttatgaatt gatggataaa aagcggaata tcctgatcac
     5581 agaattgaca ggcctgattt ccgaggcaaa aagtattcag tctgagatcg atatgacgtt
     5641 cacaaaggca tatgaagcac tgcaggttgc aaatatcgag ttaggaatca gttatgtcca
     5701 ggagatcgca aaagcgatgc cggtcgaaga ttctattgtt attaaaacac gaagcgttat
     5761 gggcactgaa attccgagag tggaaatgca gaatatcgat acgaagccga catatgcgtt
     5821 ttacagtacc agatattctc ttgataaagc aagagaagca tttcaaaaag taaaggaact
     5881 gacagtccgg ctttccgccg tagaaaattc tgcttatcgt ttggcggcta atatccggaa
     5941 gacgcaaaaa cgtgcaaatg cactgaacaa tattacgatt cctacttata cggcgttatg
     6001 taaaaatatt caggccgcac tggaggaaaa agaacgggaa gagtttacga gattaaaggt
     6061 tataaaaagg aataaacaaa aagaagcctg atttgaaggc ttcttttttt gtgaacaaac
     6121 ttaagaaaaa aaggttttaa cttgccagtc atataaattt cgcctatact actaaaggag
     6181 tattttatca tttatagaaa taggaggagg gaacatggat tctaaaacaa tccagatttt
     6241 aactgcgatg acgatctata tggcggcggt gatcattatc ggaattgcat ttgcaaaaaa
     6301 ggctaataaa agttctgaaa attatttttt agggggcaga actttagggc cgtgggtaag
     6361 tgccatgagt gccgaggctt ccgatatgag cggctggctt ttaatgggac tgccaggcgt
     6421 tgcgtattgg tgcgggcttg cagatgcagc atggacggca atcggactgg caatcggtac
     6481 ttatattaac tggctcgttg tttctaaaag gcttcgccgc tacagtatca aggtaaatgc
     6541 gatcacactg ccggagtttt tttcaaaccg gtatgatgaa gaca
//
LOCUS       DAOSYE010000013         6486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7806_length_6486_cov_3.483595, whole genome shotgun sequence.
ACCESSION   DAOSYE010000013 DAOSYE010000000
VERSION     DAOSYE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_6486_cov_3.483595"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLU43_00480"
     CDS             complement(<1..316)
                     /locus_tag="PLU43_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26910.1"
                     /translation="MKKKLLSVLLSAAMVAAMLTGCGASGSAASEDTDTAEAASETEA
                     ADSTEAETADTAAAEETADAAEAESSASGDVTIWYYWETEGHQKALNHIIEEYNNSQD
                     SIK"
     gene            complement(426..1973)
                     /locus_tag="PLU43_00485"
     CDS             complement(426..1973)
                     /locus_tag="PLU43_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO26911.1"
                     /translation="MRIVVVEDEIRIREGICKLIKRIDATHQIAGVAETGEEGLALIL
                     EKKPELVITDVKMPKMDGLQMLKALKQAGFSTKVIILSAYSEFEYAKQAMMLDVKEYL
                     LKPIDIVELKAALSRIAEQYRFELQENKNTVKSLPYLCTGIILGTLSVSPEIESILQK
                     EYLLYPKTKLLLFQIYLGKAYERKRKKTERYVEKFYETHQEIRGCVLKLPKDKSILLI
                     VYGYEDMHQWERRFQFQIMNDCRQSVADEKHREEMSFGFTECGGITALREAYQTLYHY
                     MEWNIILGDEVMVSYPKVSHINTVPCTYPIDIAGRLKTALCIGDLEKVRKIIEDFQNY
                     FSDGSVYQPKEIKETYIRFLWTLISIAKDINLIKEGQIEMQAVLEQVTEAKTAGELHA
                     VMRQVWEQFEGEKEEEAKLSLTVKRAQSMIHEFYQTGITLEEISNKLNITPEYLGTQF
                     KKEIGVSFSGYIREYRLNKAKKLLLSTQLKLYEIAEAVGYTDPKYFSRQFKEYVGQSP
                     GDYQKTH"
     gene            complement(1973..2323)
                     /locus_tag="PLU43_00490"
     CDS             complement(1973..2323)
                     /locus_tag="PLU43_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO26912.1"
                     /translation="MECRIHKLLLQPFIENTIIHGFEKSSEIHKLFVGMKEEGEFLCI
                     TIADSGKGMEEALVKQLNEGVLLQTEGKYSIGIRNAVSRIKMYYGEVSKVTITSSPGK
                     GTKIEIHIPKEAML"
     gene            complement(2375..3778)
                     /locus_tag="PLU43_00495"
     CDS             complement(2375..3778)
                     /locus_tag="PLU43_00495"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO26913.1"
                     /translation="MKKINFKKYNTQLILSFLCVSVIPILIVSMLSYYIGMRTIRNSM
                     NEMIEMNLKQTQNSLEIWLDSYEDILFQLYTDEDVIALVEDINNQKDTAVARSQLKRK
                     LRSMFFTKDYVKAITIISRTGEIIDYDMLVSSTEETSWMDHYDMSREELYEKLSDGNK
                     VKILPTEYATTFANEEYYLFHIGNRLINYKKPDQDIGVIIVSIDEKLLQGICLTEDED
                     SNKNNFHFLVDNDGNIISYIDTSKIHLKISDTSRSNDEKLQDYLQFIKQTKEFEKTDV
                     SVSSCLAQKPLWNIVHVSNQEQYVKELRDKQKLIILVIIVSMLGVVMIITLLSRKLTQ
                     SINRIVEAIKRAGTGNLSEPIAPDKRMPEEIETIAVQFNHMLEELDRSMTREKKAYDR
                     QRIAEIAALEAQINPHFIYNTLDTINWMAIDHDEYEISNAINALAKILRYGIDNSNGV
                     VTIREEAEWLKQYIFLQ"
     gene            complement(3765..4661)
                     /locus_tag="PLU43_00500"
     CDS             complement(3765..4661)
                     /locus_tag="PLU43_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO26914.1"
                     /translation="MTKNSVLLQLNQMPLVMVADRIFAKKPFLHTDRTADFHVLIYVL
                     KGRISVIEGREEHHVSAGKLLLLKAGIRHYGVTFSAPDTEWIYVHFLLEQPEQPIMEF
                     QPYTSHLQEQIFSDKDYAYTLELPKMSDIGKGSVTDRKLRNLVGIFHSKGPLRAGYMN
                     PLFVEILLDCYQSVKEEADVQNTDKVYEIIQYLENHTRENFDTALLEEQFHLSYKHIG
                     RFFKAATGRTLLEYHTKLRMNEAARLLRESTEQISVISRDMGYPDPFYFSNVFRKWMG
                     VSPKFYREQYIRKGYGAIYEED"
     gene            4823..>6486
                     /locus_tag="PLU43_00505"
     CDS             4823..>6486
                     /locus_tag="PLU43_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOO26915.1"
                     /translation="MLNFMKNFQMNDPFFSRYETLIKEAVIPYQEKALNDEIPGAEKS
                     HAIANFKIAAKYLQSGEQPEDFYGMVFQDSDIAKWLEAVSYSLILFPDKELEARADAL
                     IDLIGSAQQADGYLNTYFTLHDDKKHFSNLHEAHELYCAGHMMEAAVAYFESTGKRKL
                     LDIMLKMTDCIYQHFIVEKAPGYPGHPEVELALMRMYDVTGSKKCLTLAAHFINVRGV
                     DPDYFEKERKTTGWMIWGGDPSDHDYAQNAAPLREQSDAVGHAVRAVYLYTGMAALAK
                     ETGDTKLKNACIRLWESITKKRSYITGGIGSTYLGEAFTTDYDLPNDTAYAETCASIG
                     LIFFARKMLDLECDSTYSDMMERTLYNGVLSGMQLNGQRFFYVNHLESIPGISGTIAT
                     HKHTLPERPQWFGCACCPPNIARLLPSISEYAWGETASTVFSHLYLSGDLTLERKQVT
                     IRTQSAYPYDDVVHYSFIPKQESAKFTFAIRIPDWSRHTQVLLNNNVIYEKTNTSESI
                     LPQNSADVSAASDKPDDFQTTCKNGYLYITRSFSKEDVLTLKLDMTP"
BASE COUNT         1652 a         1601 c         1253 g         1980 t
ORIGIN      
        1 ctttaatgct gtcctgagag ttattgtatt cttcgataat atgatttaat gctttctgat
       61 gtccttctgt ctcccagtaa taccagatgg tcacatcccc ggatgcgctg ctttccgctt
      121 ccgctgcgtc ggctgtttct tctgctgccg ccgtatcggc tgtttctgcc tctgtgctgt
      181 ccgctgcttc cgtttcggac gctgcctctg ccgtatccgt atcttccgat gccgcagaac
      241 ccgatgctcc gcatcctgta agcatggcag ctaccatggc agccgataac aatactgata
      301 ataatttctt tttcatactg acctccttgt ttgtcagaat cacactgacc gtctttgtta
      361 tgaaataact ataacggttc tttttgcagg tataaacgca gtatttctaa gaaaggataa
      421 caaatctaat gtgttttctg ataatctcct ggcgactgcc cgacatactc tttaaactgc
      481 cggctgaaat atttcggatc cgtatagcct actgcttccg ctatttcata aagcttcagc
      541 tgtgtggaaa gcagcagttt ttttgcttta ttcaaccgat attcacgaat ataaccgctg
      601 aatgaaacac cgatctcctt tttgaactgt gttcctaaat attccggcgt tatattcagt
      661 ttattcgaaa tttcctccaa tgtgatgcct gtctgataaa actcatggat catgctctgc
      721 gctcttttga cagtcagact cagctttgcc tcttcctctt tttcaccttc gaattgttcc
      781 cagacctgcc tcataacggc atgcagttca ccagctgttt ttgcttccgt cacctgctct
      841 aagaccgcct gcatttctat ctgtccttct tttatgagat tgatgtcttt cgcaatactg
      901 atcaacgtcc agagaaatct gatatatgtt tctttgattt cctttggctg gtaaacactg
      961 ccgtcagaaa aataattctg gaaatcctcg ataattttcc tgaccttttc cagatcaccg
     1021 atacataatg ccgtcttcag tcttccggcg atatcgatcg gataagtaca gggaaccgta
     1081 ttgatatgag atactttcgg ataggagacc atgacttcat cgccgagtat gatattccat
     1141 tccatataat gatacagtgt ctggtaggct tctctcaaag ctgtgatccc gccgcattcc
     1201 gtaaagccaa agctcatttc ttccctgtgc ttttcatccg caacactttg cctgcagtca
     1261 ttcatgatct gaaactgaaa ccggcgctcc cactgatgca tatcctcata tccgtaaacg
     1321 atcagcaaaa tgcttttatc tttcggaagc ttcagtacac agcctctgat ctcctgatgc
     1381 gtttcataaa acttttccac gtaacgctct gtcttttttc tttttctttc atatgctttc
     1441 ccaagataga tctgaaaaag aagcagcttt gttttcggat agagcagata ttctttttgc
     1501 aggatacttt ctatttccgg gcttacggac agggtcccga gaatgattcc ggtacacaga
     1561 tacggaaggc tttttaccgt atttttattt tcctgcagct caaatcggta ttgttccgct
     1621 attctggaaa gtgcagcttt cagttcaacg atatcgatcg gtttcagcag atattctttt
     1681 acatccagca tcattgcctg ctttgcatat tcgaattccg agtaggcact taatataatg
     1741 acttttgtgc tgaagcctgc ctgcttaagc gccttcagca tttgcagacc gtccatcttc
     1801 ggcatcttca catcggtgat cacaagttcc ggctttttct ccagtatcaa agccagtccc
     1861 tcttcgcctg tctccgccac gccggcgatc tgatgcgtcg catcaattcg cttgatcagc
     1921 ttgcagatcc cttcccgaat tcgtatttcg tcttctacta caacgatcct catcagagca
     1981 ttgcctcctt tggtatatgt atttcaattt ttgtgccttt cccggggctg cttgtaatcg
     2041 taacctttga cacttctccg taatacattt ttattctgct tacggcattt cggattccaa
     2101 tactgtattt tccttctgtc tgtaaaagca ctccttcatt cagctgtttg acaagcgctt
     2161 cttccatgcc ctttccgctg tctgcaatcg taatgcaaag gaactcccct tcctccttca
     2221 tccctacgaa cagtttatgg atctcggagg atttttcaaa tccgtgaatg atcgtatttt
     2281 ctatgaacgg ctgcaaaagc agcttgtgta ttctgcattc catcagctgc ggctctatcg
     2341 aaatttcgct ttgaaaagaa ctcttcagac gtgtctactg taaaaagata tattgcttca
     2401 gccactccgc ttcttccctg atcgtaacga ccccattgct gttgtcgatc ccatagcgca
     2461 gtatttttgc aagcgcattg atcgcattgc tgatctcata ttcatcatga tcgattgcca
     2521 tccagttgat cgtatcaagc gtattatata taaaatgcgg attgatctgt gcttccagcg
     2581 cagcaatttc ggcgatccgc tgcctgtcat atgccttttt ttcacgcgtc atggatcgat
     2641 ccagctcctc cagcatatga ttgaactgga ccgctatcgt ctctatctcc tccggcattc
     2701 ttttatccgg tgcgatcggc tccgacaaat tacccgttcc tgcacgtttg atcgcctcta
     2761 caatacggtt gatggactgt gtcagctttc ttgatagcag cgttatgatc atgacaacgc
     2821 cgagcatgga aacgataatg accagaatga tcagtttctg cttatcccgc agttctttta
     2881 catactgctc ctgattggat acatgcacaa tattccataa aggcttctgc gccaggcagc
     2941 tgcttacgga aacatccgtc ttttcaaact cctttgtttg tttaatgaat tgcaggtaat
     3001 cctgcagctt ttcatcattg cttctgcttg tatcacttat tttgagatgt atttttgacg
     3061 tatcaatata tgaaataata tttccatcgt tatctactaa aaagtgaaaa ttatttttat
     3121 tggaatcttc atcttcagtc aggcaaatgc cctgcagcaa tttttcgtca atgctgacaa
     3181 taatcacgcc aatatcctga tccggttttt tataattgat cagccggttc ccgatatgaa
     3241 acagatagta ctcctcattg gcaaatgtcg tcgcatactc ggtcggcaag atcttcactt
     3301 tattgccgtc ggacagcttc tcatacaatt cttccctgct catatcgtaa tgatccatcc
     3361 acgacgtttc ctccgtagaa gatacaagca tatcatagtc gataatttct cctgttctgg
     3421 aaatgatcgt aatggccttt acataatctt tcgtaaaaaa catgctgcgc agctttcttt
     3481 tgagctgact tcttgccact gccgtatcct tctgattatt gatatcttcc acaagtgcga
     3541 tcacatcctc gtccgtatag agctggaaaa ggatatcttc ataggaatcc agccatattt
     3601 ccagactgtt ctgcgtctgc ttcagattca tttcgatcat ttcattcatg ctgttacgga
     3661 tcgttcgcat accgatataa taagaaagca tggaaacgat caggatcgga ataacggaga
     3721 cgcataaaaa ggacagaatc agctgggtat tatacttttt aaaattaatc ttcttcatag
     3781 atcgcgccgt acccctttct gatatactgc tctctgtaga atttgggaga aacgcccatc
     3841 catttccgaa atacattgct gaaataaaat ggatccggat agcccatatc acggctgatc
     3901 acagatatct gctccgttga ttccctaagc agcctggcgg cttcgttcat ccgcagcttt
     3961 gtatgatatt ccagcagtgt cctgcccgtc gctgccttga aaaaacggcc gatatgttta
     4021 tagcttaaat gaaactgctc ttccagcagc gcagtatcaa aattttctct tgtatgattt
     4081 tcaagatatt gtatgatctc gtaaacctta tccgtattct gcacatcagc ttcttctttc
     4141 acggactgat aacagtccag cagaatttcc acaaaaagag gattcatata tcctgcgcgc
     4201 agaggtccct tagaatgaaa aatcccgaca agattacgca gcttcctgtc tgttacactt
     4261 cccttcccaa tgtccgacat tttaggaagc tccagtgtat aggcataatc cttatcggaa
     4321 aagatctgct cctgcagatg cgacgtatat ggctgaaatt ccatgatcgg ctgttccggc
     4381 tgttccagca gaaaatgcac atagatccac tccgtatccg gcgcactgaa cgtcactcca
     4441 taatgcctga tcccagcctt caaaaggaga agctttccgg cactcacatg atgttcttca
     4501 cggccttcga taacggaaat acgccctttt aaaacataga tcagaacatg aaaatcggcg
     4561 gtcctgtccg tatgaagaaa cggttttttc gcaaaaatac ggtcggcaac cattacaagc
     4621 ggcatctgat tcaattgcag caatactgaa ttttttgtca tttttccgta ctcctttctt
     4681 tccatttccc aatatcagtt ccgttttctt gaaaaagcga ttattttata tgttattgca
     4741 attattacct atttttctcc ctgtttaata tgattataat acatatcgta acgaaaatac
     4801 aatagataag gagatattcg ccatgttaaa ttttatgaaa aactttcaaa tgaatgatcc
     4861 ttttttcagc cgatatgaga ccctgataaa agaagccgtt attccgtatc aggaaaaagc
     4921 gctgaacgat gaaattccag gcgcagaaaa aagccatgca attgcaaatt tcaaaattgc
     4981 tgcaaaatat ctgcagtcag gagaacaacc ggaagacttt tacggcatgg tctttcagga
     5041 cagtgacatt gcaaaatggc tggaagcagt ttcctacagc ctgatcctgt tcccggacaa
     5101 agaactggaa gcccgggccg atgcactcat tgatctgatc ggaagcgcgc agcaggcaga
     5161 tgggtatctg aatacttatt ttacactgca tgatgataaa aaacattttt ccaatctgca
     5221 tgaggcacat gagctctact gtgccggaca tatgatggag gcagctgttg cctattttga
     5281 aagcaccggc aagcgcaaac tgctggacat catgctgaaa atgacagact gtatctatca
     5341 gcatttcatt gttgaaaagg caccgggata tccgggacat cctgaggttg agctggcact
     5401 catgcgcatg tatgatgtga ccggttctaa gaaatgcctg acacttgccg cacattttat
     5461 caacgtacgc ggcgtggatc ctgactactt tgaaaaagaa agaaagacga ccggatggat
     5521 gatatggggc ggtgacccaa gcgatcacga ttacgcgcaa aatgccgctc ccctgcggga
     5581 acaaagtgat gccgtcggac atgccgtacg ggcggtatac ctctatacgg gcatggctgc
     5641 tctggcaaaa gaaacaggag acaccaaact gaaaaacgca tgtatccggc tgtgggaaag
     5701 tatcactaaa aaacgctctt atattacagg cgggatcggt tctacctacc tgggagaagc
     5761 ttttacgaca gattatgacc tgccgaacga taccgcatat gcggaaacct gcgcttccat
     5821 cggactgatt ttctttgccc ggaaaatgct tgacctggaa tgtgacagca cctattccga
     5881 catgatggag cgtacactct ataatggtgt cctttccggc atgcagctca atggccagcg
     5941 attcttttat gtaaaccatc tggagtccat ccccggcatt tccggaacga tcgcaacgca
     6001 taaacacacg cttccggaac gtccgcaatg gtttggctgc gcctgctgtc cgccgaatat
     6061 tgcacgcctg ctgccttcca tttccgaata tgcatggggc gaaactgcat cgactgtttt
     6121 ctctcatctt tatctttccg gcgacttaac gctggaacgc aagcaggtga cgatccgtac
     6181 gcaaagcgct tatccttatg atgatgttgt tcactacagc tttatcccca aacaggaatc
     6241 tgcaaaattc acatttgcga tccgtattcc ggactggagc cgtcataccc aggtattatt
     6301 aaataacaat gttatttatg aaaaaaccaa cacttcagag tcgatcctgc cgcagaattc
     6361 tgccgacgtc tctgcagcgt cagacaagcc ggacgacttt cagacaacat gtaaaaacgg
     6421 ttatttatat attacacgca gcttttcaaa agaggatgtc ctcacactga aattagatat
     6481 gactcc
//
LOCUS       DAOSYE010000014         6468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7838_length_6468_cov_3.109933, whole genome shotgun sequence.
ACCESSION   DAOSYE010000014 DAOSYE010000000
VERSION     DAOSYE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_6468_cov_3.109933"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3436
                     /locus_tag="PLU43_00510"
     CDS             <1..3436
                     /locus_tag="PLU43_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOO26916.1"
                     /translation="ERIMEKYRYNRNVLRNAYPSVEECLCYGENSAFLWNRSTEDQDF
                     FVYRLGVGNMALETSITLPKERFSVTEDTLKLLPMHLKEKYGVLRDVPVCIDLKEEHL
                     HGILGSSEEELREVLLLLAMQIAVTSSAQQVCFAFLFLDKKRYKKCMEVFKWLPHVWL
                     GEERLIAWESAHIAETVRRLEQTKKCLEPDMRHLFIFTDHYDLPGHELQCCEEVSVLL
                     FTDDYGEIPGCCHSMILRTAAFSGKLTINDKHTERNEIYFDRFSLKKAERYARQLCGL
                     KLEEVKTQKKIPEKVTIFELLGISDIDWRDIYDNWQVNRSEAGLPVGIGMAEGGRICI
                     LDLHEKGHGPHGLIAGMTGSGKSEMLQTIIISLAMKFSPWEVGFFLIDYKGGGMAGLF
                     SKLPHLLGSISNLSGSMIYRAMVSIRSENERRQRIFLKAGVNQISDYHKLYRNGKAAF
                     TLPHIFIMIDEFAELKREEPDFMRELISVAQVGRSLGIHLILATQKPSGTVDDNIWSN
                     ARFRICLKVQDRQDSSDMLHKPDAAYIKNPGRAVLQVGNHELYEMFQGAYTMEPCAEK
                     ETLHETETVCLLDEFGRQSSRYLPPGTYQGEAAEQREPQIIKMLEAVFTAAVHCKFEK
                     LTGLWLPPLDSRIFLSELVLHDSKGDQADNSTDRMIYRISVGKYDHPKKQQQGIFQIS
                     LPDGGHHVICGTAACGKSTFLQTVLYSFIYQENPETLQIYIIDYSSGLLAGFAKSALV
                     GGVIAEEEGDELPKLFCMLARLHAERKRLLRGSSFCQCLNMPAVLLVIDNYGSFREKT
                     GACYDSLLQELAKTGENDGIYLFLTASGVGNNGLPGRIFENVRTGICLSMNDKYRYCE
                     ILRISHLQLRLPENIKGRGMALIGGEVLEFQCALCAEGENDFERMENIQKMIDVRNRT
                     YCGRRVPKIPRIPKNSTLTKFFEVLEERQTDMSAGLPAGYEEISGKIYLIPVFEIKHV
                     MITGRRRTGKKNMLSVIKKTAEKYGFTCSEIKTAAGLAACIKKNEKWNGKGFLYIIND
                     FAEFINEFYSVSYEQEIERLLMDIFGRKSLHKIVAAVKNEDIGKLAGRGLFEVFHSDV
                     YGIHMGGRLDSQNLLDFSYLPFSRQSIAKKAGQGTVSKLFDPLFCGDISIPLDEDL"
     gene            3460..3690
                     /locus_tag="PLU43_00515"
     CDS             3460..3690
                     /locus_tag="PLU43_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26917.1"
                     /translation="MVFVDIRIPQLAKTYDFRLDEKTETAVLIRQTSVILFGKEVQGA
                     LFDLTQKQILCGNRTLTGQGTVSGHTLLLLFI"
     gene            3718..4017
                     /locus_tag="PLU43_00520"
     CDS             3718..4017
                     /locus_tag="PLU43_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26918.1"
                     /translation="MDIFKAEFAYMNRKAFRLAGLCASLELRIEELRESAGRLELFWD
                     GEANAAFILAMEEDFFKICVLQTYVKQAAGLLIMAVMDYQKTELIIQQKIGGIKI"
     gene            4014..4313
                     /locus_tag="PLU43_00525"
     CDS             4014..4313
                     /locus_tag="PLU43_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26919.1"
                     /translation="MKSKEEIEIDFSRAIAQAQELEDIAKELSQIASAHIAGALEMLA
                     LNWQGENAKQYADKGKILTKDMFETADDLIKVAKNIRLTAGIVYDAEKTAVGIGY"
     gene            4359..4658
                     /locus_tag="PLU43_00530"
     CDS             4359..4658
                     /locus_tag="PLU43_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOO26920.1"
                     /translation="MSFIQVTASELRNRAEELRGLNARFKSQAETLETCEQTLKSMWE
                     GQANDVFHSAFIRDKGQMDGFYGAIEQYVSALLVIAEKYEEAETKNTATASARSY"
     gene            4695..5009
                     /locus_tag="PLU43_00535"
     CDS             4695..5009
                     /locus_tag="PLU43_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOO26921.1"
                     /translation="MEGILKVTPEKLIQTSNEFGTTGKAISSLTQEMTAIVNGLKSIW
                     QGDAASTYGNKFSGLQDDIEKINRIIQEHVDDLSLMAREYQNAENAGVEESSRLLTEV
                     VS"
     gene            5037..6335
                     /locus_tag="PLU43_00540"
     CDS             5037..6335
                     /locus_tag="PLU43_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO26922.1"
                     /translation="MEERYKTLILVDNSLSVKKGSQQIIKETLTYLFEHAGDEEDFSL
                     AVFGEEISYLKEYGTDRQELITAAEQIVFENKDTYITDVLMNVVNEWREADFACRSIL
                     LVTDGLTEESSEYPDEELYFTLDKTDYPVYVIGCVQNDNQTVMKHLAAIARISSGEIF
                     YTEFEDSEASVECKIGDAVLAAMLNRHNEERADENYRSEEEEPLSAEEKVSENSLLLS
                     DSMDTEAVIYEKEISKDELFLHGRSGPMLFLGITSVLLVLLSVCFFMVKKNLKRKRHE
                     KKFMEDLKSEVKRHSQYLKSDDEEQYGVTQALHEKTAGVDENATRCLFGPSEMQDITL
                     EDRSDPTKFFRTSCIDRLVLGRSLNMCDLVIDYDDSVSGRHCEICMRESGWFVRDLQS
                     SNGTLVNGQKVYQELELHSGDVLQLGRLRFIVRMQDTGYK"
BASE COUNT         1990 a         1085 c         1779 g         1614 t
ORIGIN      
        1 agaaaggatc atggagaaat atcgttataa cagaaacgta cttagaaatg catacccttc
       61 ggttgaagaa tgtctgtgtt atggagaaaa ttcggctttt ctatggaatc gttcgacgga
      121 ggatcaggat tttttcgtat atcggcttgg ggtcggaaat atggcattgg aaacgtcaat
      181 cacactcccg aaagagaggt tttcggttac ggaagatacg ctgaaactgc ttccgatgca
      241 tttgaaagaa aaatatggag tgcttaggga tgttccggtt tgtattgatt taaaggagga
      301 gcacctgcat ggtattttag gaagcagtga agaggaattg cgggaagttt tgctcttgct
      361 tgccatgcag atcgctgtta caagcagtgc gcagcaggta tgttttgcgt ttttgttttt
      421 ggataaaaaa aggtataaaa agtgcatgga ggtatttaaa tggcttcccc atgtatggct
      481 gggagaggag agactgatcg catgggaaag tgctcatatt gcagagacag tcagacgtct
      541 ggaacagacg aaaaagtgtc tggaaccgga tatgaggcat ctgtttattt ttacggatca
      601 ctatgatctg ccggggcatg agcttcaatg ctgcgaagaa gtttccgtgc tcttgtttac
      661 ggatgattac ggagaaattc caggctgctg tcattccatg atattgcgga cagcggcatt
      721 cagcggaaaa ttgacaatta atgataagca tactgaacgg aacgaaattt attttgacag
      781 attcagtctg aaaaaggcgg agcgttatgc caggcagtta tgcggactga aactggagga
      841 agtaaagacg caaaaaaaga ttcccgaaaa agtaacgata tttgaactgc tgggaatttc
      901 tgatattgac tggagggata tttatgataa ttggcaggta aatcgttcgg aagcaggtct
      961 gccggtcggg atcgggatgg cggagggagg acggatctgt attcttgacc tgcatgaaaa
     1021 gggacatggt cctcatggac tcatagcagg aatgacagga agcgggaaaa gcgagatgct
     1081 ccagacaatc atcatttcgc ttgccatgaa attttcgcca tgggaggtcg gcttttttct
     1141 gattgattat aaaggcggcg gaatggccgg tctgttttca aagctgcctc atcttcttgg
     1201 cagtatttca aacctttccg gcagtatgat ttaccgggcc atggtttcaa tacgaagcga
     1261 aaacgaacgc agacagcgta tttttttgaa ggccggagtc aatcagattt ccgattatca
     1321 taaattatat agaaatggaa aagcagcgtt tacattgccg catattttta ttatgatcga
     1381 tgagtttgcg gaactgaaaa gggaagagcc ggactttatg agggaactga tcagtgtggc
     1441 acaggttggc agaagtcttg gaatccatct gattcttgcc acgcagaaac catccggtac
     1501 ggtagacgac aatatatgga gcaatgccag atttcgtatc tgtcttaaag tacaggacag
     1561 acaggacagc agcgacatgc tgcataagcc ggacgccgct tatattaaaa atcccggcag
     1621 ggcggttttg caggttggca accatgaatt atatgaaatg tttcagggag cgtatacgat
     1681 ggagccgtgt gccgaaaaag aaacgcttca tgaaacagag accgtgtgtc ttcttgatga
     1741 gttcgggcgg caaagcagcc ggtatctgcc gccggggaca tatcaaggag aagctgcaga
     1801 gcagcgggaa ccacagatca ttaaaatgct ggaggcggtt tttacggcag cggtgcactg
     1861 taaatttgaa aaacttacag gattatggct gccgccgctt gacagcagga tatttttatc
     1921 tgagcttgta ttgcatgaca gcaaagggga tcaggcggac aattcaacag atcgtatgat
     1981 ttatcgaata tcggtcggaa aatatgatca tccgaaaaaa cagcagcagg gtatttttca
     2041 gatcagtctg ccggatggcg ggcatcacgt gatatgcggc actgccgcat gcggaaagag
     2101 cacatttctg caaacagttc tttattcatt catttatcag gaaaatccgg aaacattaca
     2161 aatttatata attgattaca gcagcggact gcttgccggc tttgcaaaaa gcgcgcttgt
     2221 aggaggtgtc attgccgaag aagaaggaga tgagcttcct aaattatttt gtatgcttgc
     2281 aaggctgcat gcggagagga agcggcttct gcggggaagc agtttttgtc agtgcctgaa
     2341 tatgccggca gtgctgcttg tgatagacaa ttacggaagc tttcgggaaa agacaggtgc
     2401 ctgttatgac agcctgctgc aggagcttgc aaaaacagga gagaatgatg gaatctattt
     2461 gtttttgaca gcgtccggag taggaaataa cgggcttccg ggaagaatat ttgaaaatgt
     2521 caggacaggg atctgtctgt ccatgaatga taagtaccgt tattgtgaaa tattgcggat
     2581 ctcacattta cagcttcggc tgcccgaaaa tatcaaggga aggggaatgg ctctcatagg
     2641 gggagaagta ctggaatttc agtgtgcgct ttgtgcggaa ggagaaaatg attttgagag
     2701 aatggaaaat atacaaaaga tgatcgatgt ccgtaaccgg acatattgtg ggcggcgtgt
     2761 tccaaagatc ccaagaattc caaaaaactc cacgctgaca aaattttttg aagtattgga
     2821 ggaacggcag accgatatgt cggcaggact gcctgccggg tatgaggaaa taagcgggaa
     2881 gatttatttg attcctgttt ttgaaatcaa gcatgtcatg atcaccggaa gacggcggac
     2941 agggaagaaa aatatgcttt ctgttattaa aaaaacagca gagaaatatg gatttacgtg
     3001 cagtgagatc aaaacagcgg ccggacttgc agcatgcatt aagaaaaatg aaaagtggaa
     3061 tggaaaagga tttttgtata tcatcaacga ttttgcagag tttatcaatg aattttacag
     3121 tgtttcttat gaacaggaaa tcgaacggct tcttatggat atttttgggc ggaaaagctt
     3181 acataaaatt gtcgcggcgg taaagaacga ggatatcggg aagctggcag gcagaggttt
     3241 gtttgaagta tttcactcgg acgtttatgg gatccatatg ggaggcaggc ttgacagtca
     3301 gaatctgctg gatttctctt atctgccttt cagccgtcag agtattgcga aaaaggccgg
     3361 acaggggacc gtatcaaagc ttttcgatcc gctgttctgc ggagacatca gcataccgct
     3421 ggatgaggat ttgtaacaga ggatgggaag gaagagaata tggtgtttgt ggatattcgc
     3481 attccgcagc ttgctaaaac atatgatttc aggctggatg aaaaaacaga aacagcagta
     3541 ctgatacggc agacatctgt aattttattt ggaaaggagg tgcagggggc attgtttgat
     3601 ctgactcaga aacagatcct gtgcggaaac cggacgctga ctgggcaggg aaccgtaagc
     3661 ggacatacgc tgctgctgtt atttatatag atcaatagta aacgaaggag gctcaaattg
     3721 gatatattta aagcggagtt tgcatatatg aaccgtaagg cgttcagact tgccgggtta
     3781 tgtgccagtc tggagcttcg tatcgaagaa ttgcgggaaa gtgccggccg tcttgaactt
     3841 ttctgggacg gcgaagccaa tgcagctttt atacttgcaa tggaagaaga tttttttaag
     3901 atctgcgtat tgcagactta tgtgaaacag gcagccggtc tgcttataat ggctgttatg
     3961 gattatcaga aaacagagct gatcattcag cagaaaatag gaggaattaa aatatgaagt
     4021 caaaagaaga aatagaaatc gatttttcaa gggcaattgc acaggcacag gagcttgagg
     4081 atattgcaaa ggaactaagt cagatcgcga gtgcacatat tgccggcgca ttggaaatgc
     4141 ttgcgctgaa ctggcaggga gaaaatgcaa agcagtatgc ggacaaaggg aagatcctga
     4201 caaaggatat gttcgagaca gcagatgacc tgattaaagt agctaaaaat atccggctga
     4261 cagcaggaat tgtctatgat gcggaaaaaa cggctgttgg gattggatac tgaacgaatt
     4321 tctgatcagg taaataaagg aagaagggag aaaggaaaat gtcatttata caggtaacgg
     4381 catcggagct taggaatagg gctgaagaac tgcgggggct gaatgcccgt tttaaatcac
     4441 aggctgaaac acttgaaaca tgtgagcaga cgttgaaatc catgtgggaa ggacaggcga
     4501 acgatgtttt tcacagtgcg tttataagag ataaaggaca gatggatgga ttttacggtg
     4561 ctattgagca gtatgtttcc gcgctgcttg tgattgcgga aaaatatgag gaggcagaaa
     4621 caaaaaatac ggcaaccgca tctgcaagaa gctattgaaa acaggaacta tgaaaacaaa
     4681 aaggaggaag taaaatggaa ggtattttaa aagtaacgcc tgagaaactg atacagacat
     4741 ccaatgaatt cgggacaaca ggaaaggcga tcagttcgct gacacaggaa atgactgcga
     4801 tcgtaaacgg gttaaaatct atctggcagg gcgatgcagc cagcacctac ggcaataaat
     4861 tttccggact gcaggatgat atcgaaaaga tcaaccgtat tatccaggaa catgtagatg
     4921 atttaagtct gatggcgaga gagtatcaga atgcggaaaa cgcaggtgtt gaggagagca
     4981 gcagattgct gacagaagta gtcagctaaa agaaagaaga gaaagctggg cagaatatgg
     5041 aggaacgcta taaaacactg attttggtag acaattccct gtctgtcaaa aaaggcagtc
     5101 agcaaattat aaaggaaacg cttacctatc tgtttgagca tgcaggggat gaagaggatt
     5161 tttcgctggc ggttttcggt gaagaaatca gttatctgaa agagtatgga acagacaggc
     5221 aggagctgat cactgcggcc gaacagatcg tctttgaaaa caaagacacc tatattacgg
     5281 atgtgctgat gaatgttgtc aatgaatgga gggaggcaga ttttgcatgc aggagcatat
     5341 tgcttgttac ggacggactg acggaagaat cgtctgaata tccggatgaa gaattgtatt
     5401 ttacgctgga taagacagac tatccggttt atgtgatcgg ctgtgtgcaa aatgataatc
     5461 aaaccgttat gaaacatctt gcggcgattg cgaggatcag ctccggcgaa atattctata
     5521 cggaatttga agacagtgag gcttccgtag agtgtaaaat cggtgatgcg gtgcttgcag
     5581 ccatgctgaa cagacataat gaggaaagag cagacgaaaa ttacaggagt gaagaggagg
     5641 agccgttgtc tgcagaagag aaagtgtcgg aaaacagtct gctgctttca gacagtatgg
     5701 atacggaagc agttatctat gaaaaggaga tatcaaagga tgaactgttt ttacacggac
     5761 gcagcggtcc gatgttgttt ttgggtatca catcagtgct gctggtcctg ctttcagtct
     5821 gcttttttat ggtaaaaaag aatctgaaaa ggaaaagaca tgagaaaaaa ttcatggaag
     5881 acttgaaatc tgaagtaaaa agacatagtc agtatctgaa aagtgatgac gaggaacagt
     5941 atggggtgac acaggcgctg catgaaaaga ctgcgggagt tgatgaaaac gcaaccagat
     6001 gcctgtttgg cccgtccgag atgcaggata ttacactcga agacaggtcg gatcctacga
     6061 aatttttccg tacctcctgt attgaccggc ttgtactggg acgttcttta aatatgtgtg
     6121 atcttgttat cgattatgat gattctgtat caggcaggca ttgtgagatc tgtatgaggg
     6181 agagcggatg gtttgtaaga gatttgcagt catcaaatgg gacgctggtc aatggacaaa
     6241 aagtttatca ggagctggag ctgcattccg gggatgtttt gcagcttggc aggctccggt
     6301 tcatagtcag aatgcaggat actggttata agtaaggagg tagtcatata tgcggcattt
     6361 gaacggaata ttgctggctg tattgattat ggcaacagga gtaagcggat gtatgaaaaa
     6421 tgaaagggaa gcgaacagga aaaaagaagc tgccaggcag tacctcaa
//
LOCUS       DAOSYE010000015         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7874_length_6445_cov_3.251487, whole genome shotgun sequence.
ACCESSION   DAOSYE010000015 DAOSYE010000000
VERSION     DAOSYE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_6445_cov_3.251487"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLU43_00545"
     CDS             <1..588
                     /locus_tag="PLU43_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO26923.1"
                     /translation="MGARNYIARELHDILGHSLVVTIKLLEVGKVFYKKNRQRACESL
                     EKAEDAIKKGFEEMKEITGRDSGRTYHTEALEREIRSMLKAVDASGIQVSFFMRGLSM
                     SIDEKVYDTLKRIATELVTNTLKHADAGKLLLLITVDRQRIVLQTMDNGRGVKNFVKG
                     NGLNGIDSRLALVSGEAKYTAEENEGFSSTISIPL"
     gene            765..1733
                     /locus_tag="PLU43_00550"
     CDS             765..1733
                     /locus_tag="PLU43_00550"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HOO26924.1"
                     /translation="MKKRFWVVLSLMTLGLTLSACSKEVQETVAEAGMEEPLIVISDE
                     EKNQGNETENTAVTQTLAGEAECGGIYEDRDLSYSVIGVRTNEYEDGTRYLIVKLEVY
                     NHSSADISFSPFDTLTLYGGETEYPVDILADVDIDLEGTITPGNKIIGEAAFDITESG
                     DDNYVLYTGSGFEYSPAIKISAGDIGKTFAEQFEASGVKSDYTTGVPVESEQLTILLN
                     SAQVIPSDEEGREILLLDISVTDNEDEDLSFMLGLNFGGVYTAEGIQLESEVTEWTLP
                     LDVIAHETINGIASFYIDSGTTEFYMTVTPNLDDNLNKENIVFSVQ"
     gene            1749..2570
                     /locus_tag="PLU43_00555"
     CDS             1749..2570
                     /locus_tag="PLU43_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26925.1"
                     /translation="MKNKMISSLIIMFLLSLSLTACGKEAEEVSVQTDDSAQSTVEEQ
                     TADEPSQEPAETAETAEPEEEQVNDDNAGIDVSLEGQALLASLSNSRPDKFMISATLT
                     SYGMTSQTTIYYDDKNSRTETDVPGMGKSVLISLPDENVMYSYTEGSSIGIKMTGTDD
                     PYADEGDFGVDEEMISGIVEDPSENIVARADTLNGEDVVYVETTESEEDMGEVLIKLW
                     YSAKYSVPLKYEMYVGDQKMVTYEVTEIADGSSIDSALFQPPSDVDFQESAAWEY"
     gene            complement(2567..3163)
                     /locus_tag="PLU43_00560"
     CDS             complement(2567..3163)
                     /locus_tag="PLU43_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO26926.1"
                     /translation="MGIIRTKTPDIGDLRSSLAAYRPCCLQEAKDKDRMLYCIDRYPD
                     ILSRQNELAHFTASSWIVNHQKTKILMVFHNIYHAWAWTGGHADGDADLCTVALREAY
                     EETGIFIPMYANDIFSLEAVSVPGHMKNSGWVSAHIHLNVTYVFFADEALPLKIKPDE
                     NSGVQWFPVDQAVAASTEPEMQVIYQKLNNKLFGYSMF"
     gene            3637..4968
                     /gene="aspS"
                     /locus_tag="PLU43_00565"
     CDS             3637..4968
                     /gene="aspS"
                     /locus_tag="PLU43_00565"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOO26927.1"
                     /translation="MEFLTGVNRKDTLEISDLSAGEYLGKSVKVNGAVHTIRDMGTVA
                     FIILRKREGLLQCVFEEGVTEFDLGALKEASTIEAEGELCSQEKAPNGIEVRLKKIKV
                     LSEPTEAMPIPIAKWKMTASLETLLDLRPISMRNIRERAKFKLQEGIIRGFRDFMYKE
                     GFTEIRTPKIAAKGAEGGSNLFRLDYFNKKAVLSQSPQFYKQMLVGVFDRVYEAAPVF
                     RAEKHNTPRHLNEYTSLDFEMGYIDSFEDIMAMETAALQYIMALLEKEYAKELKLLNV
                     SLPRTDKIPTVRFDEAKKQVSEKYNRKIKNPYDLEPEEEVLIGRYYKEEYDSDFVFVT
                     HYPSKKRPFYAMDDPADPTFTLSFDLLFRGMEITTGGQRIHDYQMLLEKIAARNMETE
                     GMDCYLSAFKHGMPPHGGLGIGLERLTMKLLGESNVRETTMFPRDLSRLEP"
     gene            5023..5316
                     /gene="gatC"
                     /locus_tag="PLU43_00570"
     CDS             5023..5316
                     /gene="gatC"
                     /locus_tag="PLU43_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOO26928.1"
                     /translation="MKNVISNETIDYVGILAKLELSDAEKEQAKQDMGNMLDYIDKLN
                     ELDTTGVEPMSHVFAVNNVFREDVVTNGDDREEILKNAPAAKSGSFKVPKTVE"
     gene            5328..>6445
                     /gene="gatA"
                     /locus_tag="PLU43_00575"
     CDS             5328..>6445
                     /gene="gatA"
                     /locus_tag="PLU43_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOO26929.1"
                     /translation="MDLMSLTAVELGKKIKAGEVSVTEAAEAALQKIEEKEETYHALI
                     TVSRDRALQRAKEVQAKIDAGELTGPLAGVPVIIKDNMCTKDVLTTCASKILENFVPT
                     YDAEAVLNLEKAGAVIIAKSNMDEFAMGSTTETSYYGVTRNPHDSAHVPGGSSGGSCA
                     AVALEECSCALGSDTGGSIRQPSGFCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPIAK
                     DVTDCATLLEAIASYDKKDSTSIKRESYDFTTALHEDVKGMKIGIPRDYLSDGIDEEV
                     KEAILAAAQQFKKLGAVVEEFDLSLVEYAIPAYYVIASAEASSNLARFDGIKYGYRTE
                     EYEGLHHMYKKTRSEGFGAEVKRRIMLGSFVLSSGYYDA"
BASE COUNT         1893 a         1230 c         1792 g         1530 t
ORIGIN      
        1 atgggcgcgc gcaattatat tgccagagaa cttcatgata ttttagggca ttctctggtc
       61 gttacgatca aactgctgga ggtcggcaag gttttttaca aaaaaaatcg ccaaagagcc
      121 tgcgagagtc ttgaaaaagc agaagacgca ataaaaaaag gctttgagga aatgaaagaa
      181 attacgggca gggacagcgg ccggacgtat cataccgaag cgctggaaag ggagatccga
      241 agtatgctga aggctgtgga tgccagcggc atccaggtca gtttttttat gagagggctc
      301 agcatgtcca tagatgaaaa agtgtatgat acgctcaaac ggatcgctac ggagcttgtg
      361 accaatacgc tgaagcatgc cgatgcaggc aagttactgc ttctgataac agtggaccgg
      421 caaaggatcg tattgcagac gatggacaat ggcagaggcg tgaaaaattt tgtgaaagga
      481 aacggattga acggcataga cagcaggctt gccctggttt ctggagaagc aaaatatacg
      541 gcggaggaaa acgaaggctt ttcatcaact ataagcattc cgctgtgaaa atagcattat
      601 tttaagatcc ggatcatttc cgggtctttt tttagacagg cggccaataa atggtgccgg
      661 ccggccattt tcaaatgact gccggtatct acaaattgac agcgtacggc gttaaaatta
      721 aagttggtct ttttatttta aagcttaatc ggaaggagag agaaatgaaa aaaagatttt
      781 gggttgtatt gagtctgatg actttgggcc tgacgctgtc tgcctgttct aaggaggtgc
      841 aggaaactgt ggcagaggca gggatggagg agccgcttat tgtaatttca gatgaagaaa
      901 agaatcaggg aaacgagact gaaaatactg ctgttacgca gactttggca ggcgaagcgg
      961 aatgcggcgg tatctatgaa gacagggatt taagttatag cgtgatcgga gtgaggacaa
     1021 atgagtatga ggacgggacc agatatctga tcgtcaagct ggaagtgtat aatcacagca
     1081 gtgctgatat ttcattttcc ccatttgata cattgacctt atatggagga gaaacagaat
     1141 atcctgtaga cattctggct gatgtggata ttgatctgga gggaacgatc acaccgggga
     1201 ataagatcat cggtgaagct gcttttgata ttaccgaaag cggggatgat aattatgttt
     1261 tgtatacggg cagcggcttt gaatattcac ctgcaattaa aatcagtgcc ggcgatattg
     1321 gaaagacatt tgccgaacag tttgaagcaa gcggagttaa aagtgattat acgacagggg
     1381 tacctgtaga gtctgagcag ctgactattt tactcaacag tgcacaggtg attccgagtg
     1441 acgaagaagg cagggagatc ttgcttctgg atatttccgt tacggataat gaagatgagg
     1501 atctcagctt tatgcttggt ctcaatttcg gcggcgtata tacggcggag ggcatacagc
     1561 ttgaatcgga agtgacggaa tggacgctgc cgttagacgt gattgctcat gagacaataa
     1621 acggcattgc ttcattctat attgacagcg gtacaacaga attttatatg acggtgacac
     1681 cgaatcttga tgacaatttg aacaaagaga acattgtatt ttccgtacag taagaaagga
     1741 gaacataaat gaaaaataag atgataagca gtctgataat catgttttta ttatccctgt
     1801 cacttacggc atgtggcaaa gaggcagagg aggtttccgt gcagacggat gattcggcac
     1861 agagtactgt ggaagagcag acggcggacg agccgtcaca ggagccggca gagacagcag
     1921 aaacggcgga gccggaggaa gagcaggtaa acgatgataa cgcagggatc gatgtaagtc
     1981 tggagggcca ggcactgctg gcgtcattgt caaacagcag accggataaa tttatgatat
     2041 ctgcgacatt gacttcctat ggaatgacat cgcagacaac gatctattat gatgataaaa
     2101 attcaagaac ggaaacggac gtaccgggta tgggcaaaag cgttttgatc agcctgccgg
     2161 atgaaaatgt gatgtacagt tacacagagg gcagttcgat cggcataaaa atgacaggca
     2221 ccgatgatcc ttatgctgac gaaggggatt ttggagtcga tgaagaaatg atttccggta
     2281 ttgtggaaga tccttctgaa aatattgttg ccagagccga tacgctgaac ggcgaggatg
     2341 tcgtttatgt agagacaaca gagtcagagg aggatatggg cgaggttctg atcaagctgt
     2401 ggtactcagc caaatattct gttccgttaa aatatgagat gtatgtagga gatcagaaaa
     2461 tggtcacgta tgaagttacc gagatcgcag acggaagctc catagacagc gctttatttc
     2521 agccgccgtc ggacgtggat tttcaggaat cggccgcatg ggagtactaa aacatgctgt
     2581 agccgaacag cttgttgttc agtttctggt agatcacctg catttccggc tctgttgagg
     2641 cggcaacggc ctgatcgaca ggaaaccatt gaacgccgct gttttcatcg ggcttgattt
     2701 taagaggcag tgcctcatcc gcaaaaaaaa cataagtgac gtttaaatga atgtgcgcgg
     2761 atacccagcc gctgtttttc atatgtcctg ggacgctgac agcttcaagg gaaaaaatat
     2821 cgtttgcgta catgggaata aaaatgcctg tttcttcata ggcttcccgc agggctaccg
     2881 tacataagtc ggcatctccg tcggcatggc cgccggtcca ggcccaggca tgataaatat
     2941 tatgaaaaac catgaggatc tttgtctttt gatgattgac gatccaggag gaagcagtga
     3001 aatgcgcaag ttcattctga cgggacagaa tatcgggata cctgtcaatg cagtacagca
     3061 tccggtcttt gtctttggct tcctgcaggc agcacggccg gtaagcggca agactgctgc
     3121 gcagatctcc gatatccggt gtttttgtcc gaatgattcc cataatgcga ttccctcctc
     3181 catgtattta cctatgtaaa agcatagcac tatttttccg ctatttcaat attgtagcgg
     3241 acataattgc ataaatacgg gcagaataca ggcagaactt tgaaaaaatg cttgcatgtc
     3301 aaaaaaaagt atgctagtat aagcaggatt aaggaaaggc attgaaagag attctgttcc
     3361 acgagcctga caggaatacg gcgtcaccga ctgagagcgc tgtttgggta tacggatacg
     3421 ggaaacgctc cggagccggc tgttcgaatc agacagacca gaataggtca tggctgtgta
     3481 gggcggcacg tgacacgcgt taagtgatgc aagaggtcag acagcgtctg gcaaagcggg
     3541 tggtaccgcg gattatttga ttgattcgtc ccggaatagt ttcattctat tccgggattt
     3601 tttgtgtttt acaaacagta aaaaggagag attgttatgg agttcttgac aggtgtaaac
     3661 agaaaggaca cgctggaaat cagtgattta tcagcgggag agtatttggg aaaaagcgta
     3721 aaggtaaacg gtgccgttca tacgatccgg gatatgggta cggtagcctt cataatttta
     3781 agaaagaggg aaggactttt acagtgcgtg tttgaagaag gcgtaacaga atttgatctt
     3841 ggtgcgctga aggaagcttc cacgatagag gcggaaggcg agttatgcag ccaggaaaaa
     3901 gcgccgaacg gcattgaagt ccggctgaaa aaaataaaag tgctttcgga gccgacggaa
     3961 gccatgccga tcccgatcgc aaaatggaaa atgacggctt ccctggagac actgcttgat
     4021 ttgagaccga tctccatgag aaatatcagg gagcgcgcaa aattcaagct gcaggaaggc
     4081 atcatacgcg gttttcgtga ttttatgtat aaggaaggat ttacggagat ccggacgccg
     4141 aagatcgctg caaagggagc agagggcggt tccaatttgt tccggcttga ttactttaat
     4201 aaaaaagcag tgctttccca gagtcctcag ttttataaac agatgctggt cggtgtattt
     4261 gacagggtat atgaggcagc gccggtattc cgtgcggaaa agcacaatac acccagacat
     4321 ctgaatgagt atacgagcct tgattttgaa atgggctata ttgacagctt tgaagatatt
     4381 atggcgatgg agacggcagc cctgcagtat attatggcgc ttcttgagaa agaatatgca
     4441 aaagaactga agctgcttaa cgtatcactg ccgcggacag ataagatccc aacagtccgt
     4501 tttgatgaag ccaaaaagca ggtctccgaa aaatataaca gaaagatcaa gaatccatat
     4561 gaccttgagc cggaggagga agtactgatc ggccgctatt ataaggaaga atatgattcg
     4621 gattttgtat ttgtcacaca ttatccttcc aagaaacgtc cgttttatgc aatggatgat
     4681 cctgcagatc cgacatttac gctaagcttt gacctgctgt tccgcggaat ggaaatcacg
     4741 acaggcggtc agcgtattca tgattaccag atgctgcttg aaaaaattgc ggcaagaaat
     4801 atggaaactg aaggaatgga ctgctatttg tcggcattca agcatgggat gccgccgcac
     4861 ggcggacttg ggatcggtct tgagagactg acgatgaagc tgcttggaga gagcaatgtt
     4921 cgggaaacaa caatgttccc cagagatcta agcaggttgg aaccataggc agagacagat
     4981 gaaaaaagta aacagggaaa tcaggagaag gaggcagcac gtatgaaaaa tgttatttca
     5041 aacgagacga tcgactatgt gggaattctt gcaaagctgg agctttccga tgcggagaag
     5101 gaacaggcaa agcaggacat gggaaatatg ctggattata tcgacaagct gaacgagctg
     5161 gatacgacgg gtgtagagcc gatgtcccat gtgttcgcgg tcaacaatgt atttcgtgag
     5221 gacgttgtaa cgaacggtga cgaccgtgag gaaatcttaa agaatgcacc ggctgcaaag
     5281 agcggcagct tcaaggtgcc gaagacagtg gaataggggg catttagatg gatttgatga
     5341 gtttgacggc cgtagagctg gggaaaaaaa taaaggcagg agaagtttcg gtaacggaag
     5401 ccgcagaggc ggccctgcag aaaatagaag aaaaagaaga gacgtatcat gcactgatca
     5461 cagtcagccg ggatcgggca ttgcagcgtg cgaaggaagt gcaggcaaag atcgatgccg
     5521 gggaactgac cggtccgctt gcaggagtac cggtgatcat caaggacaat atgtgtacga
     5581 aagatgtcct gacgacctgt gcctccaaaa tactggagaa ttttgtaccg acctatgatg
     5641 cggaagcggt tttgaattta gaaaaagcgg gggcagtcat catcgcaaag tccaatatgg
     5701 atgaattcgc gatgggaagc acaacagaaa cttcctatta cggtgtgaca agaaatccgc
     5761 atgattctgc acatgtacca ggcggttctt ccggcggcag ctgcgcggca gtggcacttg
     5821 aggaatgttc ctgtgcactt ggttccgata cgggcggctc tatcagacag ccgagcggtt
     5881 tctgcggagt ggtcggcctg aagccgacgt acggtacggt atccagatat ggtctgatcg
     5941 cttatggttc ttcccttgac cagatcggtc cgatcgcgaa agacgtaaca gactgtgcga
     6001 cgctgttgga agcgatcgct tcttatgata aaaaggattc cacttccatc aaacgcgaaa
     6061 gctatgattt tacaactgcg ctgcacgaag acgtaaaagg catgaagatc ggtattccaa
     6121 gggattatct gtcggacggg atagatgagg aagtaaagga agcgattctg gctgctgcgc
     6181 agcagtttaa aaagcttggc gcagtggtgg aggaatttga tctaagcctc gtggaatatg
     6241 cgattccggc ttattatgtc attgcatccg cggaagccag ctccaatctg gcacgttttg
     6301 acgggatcaa atacggctat cgtacggagg aatacgaagg cctgcatcat atgtataaaa
     6361 agacccgttc cgaaggcttt ggtgcagaag taaagcgcag gatcatgctc ggttcgtttg
     6421 tcttaagctc cgggtattac gatgc
//
LOCUS       DAOSYE010000016         6426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_7900_length_6426_cov_3.075655, whole genome shotgun sequence.
ACCESSION   DAOSYE010000016 DAOSYE010000000
VERSION     DAOSYE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7900_length_6426_cov_3.075655"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1844
                     /locus_tag="PLU43_00580"
     CDS             27..1844
                     /locus_tag="PLU43_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOO26930.1"
                     /translation="MAEATIINQELRPWEQYRNYFIVIGVVFVLLAIFVFILIIDNMK
                     RRKIQKEISVLYQKYETAIYGTNSAVWELHLDSMTVETSRNFEEIFGKKIPYKGEMHE
                     ILEKLTDNDHGRQIIEAVQEYMKGEQTEINLRIPLADDREEQRWILIRGSGVYDLNGT
                     LTDMTGIFLDITSEKVKENYIAYFAKHDFLTGLPNRMDFEETLQKELDAGNSGVLMLF
                     DVDDFKSINDTLGHAFGDEILKQVADRLKNLCTGDRYVARMGGDEFLLILRNAKEKED
                     VLSCLEDINRVIADVFCYDEIEEYISFSAGITFYPKDNNNINQLMMNADAAMYRVKRS
                     GKNNYQFYYEEMKRELKEKKEIETVLRQAVKEGTFELYYQPQVDAETGVINGFEALIR
                     IKDFHVSPGVFIPIAEETGDIIEIGRWVAKEAISQMAEWKKKGFPEKRIAINYSAKQM
                     RDKGYLSYVNQLLQEYEIDPQNIEIEITEGILLEKTEATMEFLKRIRSMGMRLALDDF
                     GSGYSSLSYLTFIPVNKIKLDKSINDKFLDIDNMRVMESIISLAHSLELTITAEGIED
                     RDKFLKLKQCGCDMIQGYLFDRPMPAEKLEGIYNQVYGV"
     gene            1871..2806
                     /locus_tag="PLU43_00585"
     CDS             1871..2806
                     /locus_tag="PLU43_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO26931.1"
                     /translation="MGKEQEIPQRMKEAINGVFIGKEEIVHKSLICLLAGGHLLIEDV
                     PGIGKTTLAGTLAKSTGCSFGRIQFTPDTLPGDITGMSVYNMKSGEFEYRAGVIMHQI
                     LLGDEINRTSPKTQSSLLEAMAEGQVTVDGVTYLLPDPFMVIATQNPVEFLGTYPLPE
                     AQMDRFMMRLTIGYPDKEQEILMAAQFIAGNTPDKICAVCGPGDIIRLRGLVQTVTVA
                     DSVLEYMEGIVERTRKEERFRLGASPRALLALIRASQGNAFLQERSYVTPDDVKYVAD
                     SVLAHRLVLSPEAKIRKEDVSGILRSLILKEKVPV"
     gene            2817..3983
                     /locus_tag="PLU43_00590"
     CDS             2817..3983
                     /locus_tag="PLU43_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO26932.1"
                     /translation="MRIRRIVLFAVWVLSVILISFYGGTVSYGLFFAVSLILPVSFIY
                     TLCVFFRFRLYQQIQSRNIICRQPVSYYFALQNEDFYGFASIRAVMFSSLSYVEDVPD
                     NMEYELLPGDRYTYRTKLICKYRGEYEVGVREIIITDFFRLFQLKYKIPGTIKALVQP
                     RVIYLEELRSISEIAAFVRKENDLAATEPDAVVRDYIAGDRMKYIHWKASAKEQKLKT
                     RTVTGEEKQMLFLFFDTKRYGRDMTKYLPPENQVLETVLALGMFFTKRHLSFSLLQEQ
                     AGTDNRLINGLQDFEAYYKKIADTRFDSDIDERLLVNNALEQGMVNQCNMIIGVLQSV
                     TKEMLPMFASVLERGVAVVLYVVTNDPIGELLKQGNERLRIIKVPIEADLSDVL"
     gene            4010..6352
                     /locus_tag="PLU43_00595"
     CDS             4010..6352
                     /locus_tag="PLU43_00595"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO26933.1"
                     /translation="MKKGENNQKLWNYIIYRFFYAVLLFGIALSGFSGYFLIYKPNYR
                     HVLAGVTVFFLLLMWNDTGLRGRLLTILLVLAVLAAAVVFFTPARTGEFLAGYFRWLF
                     AGEKQEADRLAAYGILQTVLLSVGCYLLEVFSEHFKKVRYAAAAGFLIVMLVMMFNKD
                     RFSHMGAVLSIWYIVLSCMEWTKEHWKKEKSQDTKSYMIRMLPFCVLYVLLLSLLPSI
                     QKPFDWNFVKTAYYGVKESVTAVIENISKGDSEDFQMAFTGFSEDGSLGGNIFETKQP
                     LMTVQGNYNIKTTLYLTGKLYDTFDGRTWSTKAEPVENDGLIDTLETVYAAQRYDEKE
                     VRNYISDAKISVRYRYFKSGYLFAPIKTFDISGCAYSAVGGTFLFPDKKGYGTEYEAS
                     FWQLNLDMQEFDDFLEAELPADEMLWNTLSARYVPSAAGRPALAGLADRRDRIHETYT
                     AEPGLSFETKEYLKEIVQGSVSDIEKLRAIERELASFTYTDTPGEIPASIKNESDFLD
                     YFLEKKEGYCSYFATAFVLLAREEGFPARYAEGFCVPASSKEEEIVYSDMSHAWPEVY
                     FDGIGWIPFEPTPGYGEFRYMSWGKSSEGTAAVSGNTHVPDTDREQTAAFEESETGDR
                     QIDKRLVKIIEITGALVIILILLIFAADKMITEIRYRHMDLEHQYLTEVRKNLWLLEK
                     IGLERKSFETLRELKARGEGLLESGSAAAADHLHFLDLYEKYLYGKITITKEMLQETK
                     EQQTQLLLWLKQQRKWYYYRIRLVSGLGSLKESGKRGRKS"
BASE COUNT         1830 a         1155 c         1730 g         1711 t
ORIGIN      
        1 aataaaataa ttcctgtatt gctcccatgg ccgaagcaac gatcattaat caggagcttc
       61 ggccatggga gcaatacagg aattatttta ttgtaattgg agttgttttt gtattacttg
      121 caatatttgt ttttattctg atcattgata atatgaaacg ccgcaaaata caaaaagaga
      181 tcagcgtgct ttatcagaag tatgaaacgg ctatttatgg tacgaacagt gccgtatggg
      241 agctgcatct tgatagtatg accgttgaaa catccagaaa ttttgaggag attttcggga
      301 aaaagattcc gtacaaagga gaaatgcatg agattctgga aaaactgacc gacaatgacc
      361 atgggcggca gatcatagaa gcggtgcagg aatatatgaa aggcgaacag acagagatca
      421 accttcggat tccgctggcg gacgaccggg aggagcagag atggattttg atacgcggaa
      481 gcggagtcta tgatctgaat ggaacgctga cggatatgac cgggatcttt ttggatatta
      541 cgagtgaaaa agtcaaggaa aactatattg catattttgc caagcatgat tttctgaccg
      601 gcctgccgaa ccgtatggat tttgaagaga ccctgcagaa agaattggat gccggaaaca
      661 gcggagttct gatgctgttt gatgtggatg attttaaaag tatcaacgat acgctgggac
      721 atgcatttgg ggatgagata ttgaaacagg tagcggaccg gctgaagaat ctctgcaccg
      781 gcgaccggta tgtcgccaga atgggcggag atgaatttct tctgatcctg cgtaatgcaa
      841 aagaaaaaga ggatgtgctc agctgcctgg aagatatcaa cagggtgatc gcagacgtgt
      901 tctgttatga cgaaattgaa gaatacatca gcttcagtgc aggtattacg ttttatccga
      961 aggacaataa caatatcaac cagctgatga tgaacgcaga tgcggcgatg taccgcgtta
     1021 agagaagcgg taaaaataat tatcagtttt attatgagga aatgaagcgt gaactgaagg
     1081 aaaaaaagga gatagaaacc gtacttagac aggcagtcaa agaagggacc tttgaactgt
     1141 attatcagcc tcaggtcgat gcggagacag gtgtgatcaa tggatttgag gcactgatcc
     1201 gaataaagga tttccatgtc agtcccggtg tatttatccc gatcgcagaa gaaaccggag
     1261 atatcattga gatcggcagg tgggtggcaa aagaagcaat ttcacagatg gcggaatgga
     1321 aaaagaaggg atttccggaa aagcggatcg ccatcaatta ttccgcaaag cagatgcggg
     1381 acaagggata cctgtcttat gtaaaccaat tactgcagga gtatgaaata gatcctcaga
     1441 atatagaaat agaaatcacg gaagggatcc tgcttgaaaa aacagaggcg accatggaat
     1501 ttctaaaaag gatccgcagt atgggaatgc ggctggctct tgatgatttt ggttccgggt
     1561 attcttcact gagttatctg acctttatcc cggtcaacaa aatcaaattg gataaatcaa
     1621 ttaatgataa atttcttgat atcgataata tgcgggtcat ggagagtatt atttcccttg
     1681 cacacagtct ggagctgaca attacggcag aagggatcga ggacagggat aaatttctca
     1741 agctgaagca gtgcggatgc gatatgatac agggatatct gtttgacaga ccgatgcctg
     1801 cggaaaaatt agaaggtatt tataaccagg tatacggcgt atagagcagg ataagcaagg
     1861 gagaactttt atgggaaaag aacaggaaat tccgcagagg atgaaggaag ccattaacgg
     1921 ggtgtttatc ggcaaagagg aaatcgtaca taaaagcctg atctgtctgc ttgcgggcgg
     1981 acatctatta attgaggatg tgcccggtat cgggaaaaca acgctggcag gcacactggc
     2041 aaagagtacc ggctgttctt tcggaagaat tcagtttacg ccggatacat tgcctggaga
     2101 tattacggga atgtccgtat ataatatgaa gtccggggag tttgaataca gggccggcgt
     2161 cattatgcat cagattttgt taggggatga gatcaaccgg acttctccaa agacacagtc
     2221 cagtctgctg gaggccatgg cggaaggcca ggtgacggtg gatggtgtga cgtatctgct
     2281 tccggatccg tttatggtaa ttgccacgca gaatcctgtg gagttcttag gaacgtatcc
     2341 gctgccggaa gcacagatgg accgctttat gatgcggctt acgatcggtt atccggataa
     2401 ggagcaggaa attttgatgg cggcgcagtt tattgcggga aacacaccgg acaagatatg
     2461 tgcagtctgt ggaccgggag atatcatccg gctcaggggt cttgtgcaga cagtgactgt
     2521 tgcagacagt gtgctggaat acatggaggg aatcgttgag agaacaagga aggaagagag
     2581 gtttcgtctg ggtgccagtc cgagagcact gcttgcgctg atcagggcgt cccagggcaa
     2641 tgctttttta caggagcgtt cctatgtaac gccggatgac gtaaagtatg tggcagacag
     2701 tgttttggcc cacaggctgg tgctgtcgcc ggaggcgaag atcagaaaag aagatgtgtc
     2761 cggtatttta agaagtctga tactgaagga aaaggtgccc gtataggagg aatgtcatgc
     2821 ggatacggag aatcgttttg tttgcagtat gggtattgtc cgttatattg atcagttttt
     2881 acggcggcac ggtcagttat gggctttttt ttgctgtctc tctgatcctg ccggtttctt
     2941 ttatttatac gctgtgcgta tttttccggt ttcggctcta tcagcagata cagagcagga
     3001 atattatctg cagacagccg gtgtcgtatt attttgcctt gcagaatgaa gatttttatg
     3061 gatttgcaag tatcagggcc gttatgtttt cctcactttc ctatgtggaa gatgttccgg
     3121 acaatatgga gtatgaactt ttgccgggag atcgctatac ttatagaaca aagcttatct
     3181 gcaaataccg gggagaatat gaggtcggtg tcagggaaat aatcattacg gactttttca
     3241 gactgtttca gcttaagtat aaaataccgg gaacgatcaa ggcgctcgtg cagccaaggg
     3301 tcatttatct ggaagagctg cgcagtattt ccgagattgc ggcgtttgtc aggaaagaaa
     3361 atgatcttgc cgcaacagaa ccggatgcgg tcgtcagaga ttatattgcg ggagaccgta
     3421 tgaaatacat tcactggaaa gcttcggcaa aggaacagaa actgaaaaca agaacagtta
     3481 caggggaaga aaagcagatg ctctttcttt tttttgatac gaaacgctat ggcagggaca
     3541 tgaccaaata cctgccgccg gaaaatcagg ttcttgaaac agtactggcg ctcggtatgt
     3601 tttttacgaa acggcatctt tccttttccc tgctgcagga gcaggcgggt acggataacc
     3661 ggcttataaa cggactgcag gattttgaag catactataa aaaaattgcg gatacccggt
     3721 ttgattcaga tattgatgaa cggctgcttg tcaataatgc cctggaacag gggatggtaa
     3781 atcaatgtaa tatgatcatt ggggtcctgc agtctgtgac aaaggaaatg cttccgatgt
     3841 ttgcatcggt tttagaaaga ggagttgctg ttgtgcttta tgtagtgaca aatgacccga
     3901 tcggggaact gctgaaacag ggaaacgaac ggctgcggat cataaaggtt ccaatcgaag
     3961 cggatttgtc cgatgtgttg taaggatatg cgaaagaccg gaggctgtca tgaaaaaggg
     4021 tgaaaataat cagaagttgt ggaactatat catatatcgc tttttctatg ctgttcttct
     4081 tttcggcatt gcgttatccg gattttcggg atattttctg atctacaaac caaattaccg
     4141 gcatgtactg gccggagtga ccgtattttt tttgctgctc atgtggaatg ataccggttt
     4201 gaggggccgt ttgctgacaa tcctgcttgt tttggccgta cttgcagcag cagttgtttt
     4261 ttttacgcct gcgcggaccg gggaatttct ggccggctat tttcgatggt tgtttgccgg
     4321 cgaaaaacag gaagcagaca ggctggctgc ctatgggatc ctgcagacgg ttcttctttc
     4381 tgtcggctgt tatctgctcg aggttttttc cgagcatttt aaaaaggtac ggtatgcggc
     4441 agcagccgga tttttgatcg ttatgctggt tatgatgttt aacaaagatc ggttcagtca
     4501 tatgggtgcg gttctttcaa tatggtatat tgtgctaagc tgtatggaat ggacgaagga
     4561 gcattggaaa aaggaaaaga gtcaggatac aaagtcctat atgatccgga tgctgccttt
     4621 ttgtgtttta tatgtgctgc tgttaagcct tctgccttcc attcaaaagc cgtttgactg
     4681 gaactttgta aagaccgcat attacggagt gaaagaatcc gtgactgcgg tgattgagaa
     4741 tatctccaaa ggagattctg aagattttca aatggcattt accggttttt ccgaagacgg
     4801 aagtctgggg ggaaatattt ttgaaacaaa gcagccgctt atgacggtgc agggaaatta
     4861 caatataaag accactttgt atctgacagg taaactgtat gatacgtttg acggcagaac
     4921 atggagcaca aaagcagagc ctgttgaaaa tgacggactg atcgatacac tggagactgt
     4981 atatgcagcg cagcgctatg acgaaaaaga ggtcaggaat tatatttcgg atgcgaaaat
     5041 atctgtccgc tatcgttatt tcaaatccgg ttatttattt gcaccaatca aaacgtttga
     5101 catttcgggc tgtgcgtatt ctgctgttgg cgggaccttt ttgtttccgg ataaaaaagg
     5161 atacgggaca gaatatgaag cttctttctg gcagctcaat cttgacatgc aggagtttga
     5221 tgatttttta gaagcagagc ttccggcgga tgaaatgctg tggaataccc tgtctgcccg
     5281 gtatgtgccg tctgcagcag gcaggcctgc ccttgccgga ctggcggacc gcagggacag
     5341 gattcatgaa acatatacgg cagaaccggg actttctttt gaaacaaagg aatatctgaa
     5401 agagatcgta cagggcagtg tttccgatat tgagaagctg cgagccattg agagagaact
     5461 ggcttctttc acgtatacgg atacgcctgg tgaaataccg gcttccataa aaaacgaatc
     5521 tgattttctg gattattttc tggaaaaaaa ggaaggctac tgttcttatt ttgcaacagc
     5581 ctttgttctg ctggcgcggg aagagggatt tccggcccgt tatgcggaag gattttgtgt
     5641 tccggccagt tcaaaggaag aagagatcgt ttattccgat atgtctcatg catggccgga
     5701 agtatatttt gacgggatcg gctggatccc gtttgagccg acacccggat acggggagtt
     5761 tcgatatatg tcatggggaa aaagcagtga gggaacggct gcggtgtcag ggaataccca
     5821 tgtgccggat acagatcggg aacagacagc agcttttgag gaatcagaaa caggggacag
     5881 gcagatcgat aaacgactgg taaaaattat cgagatcaca ggtgctcttg tgattattct
     5941 gatattgctt atttttgcag cagataaaat gataactgaa attcgttatc gtcatatgga
     6001 tctggaacat caatatctga cggaagtcag aaaaaattta tggctgcttg agaagatcgg
     6061 actcgaacgg aaaagctttg aaacgcttag ggagctgaaa gccagaggag agggtctttt
     6121 ggaaagcggt tctgctgcgg cagctgatca tctgcatttt cttgatcttt atgaaaagta
     6181 tttgtatgga aagataacaa tcacaaaaga gatgcttcag gaaacaaaag aacagcagac
     6241 acagcttttg ctgtggctga aacagcaaag aaaatggtat tattaccgga tcaggctggt
     6301 atcaggcctt ggcagtttaa aagaatccgg caaaagaggc aggaaatctt gaaatcagtt
     6361 tcctgcctct ttgtatcgga ctgttatttt gtattgataa tatactggtt tacaagtgct
     6421 tccagc
//
LOCUS       DAOSYE010000017         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_8035_length_6353_cov_3.773261, whole genome shotgun sequence.
ACCESSION   DAOSYE010000017 DAOSYE010000000
VERSION     DAOSYE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8035_length_6353_cov_3.773261"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLU43_00600"
     CDS             complement(<1..784)
                     /locus_tag="PLU43_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO26934.1"
                     /translation="MAVKIVNLVKRYDRTIILDHLSVEAQEGEIFGLFGTNGSGKTTL
                     VNCLMGILKYDKGEITIDGEKLFPGNLELKRKIGFVPQEIAVLKELTVYENIAYFCSL
                     YVDERSREKELAEEAVKMCGLSEVRTLLPSKLNFSFLKRLNMACGIAGAPKLLVVDEP
                     VSGVDSMTKELLLQAIRVLNKQGTTVLYASNDIEELAHICSRMAILFKGRVILTASEE
                     ELKALVGEDEKVVIEVFRITSSVLEEIEKMPGVSYVNCLNNEV"
     gene            866..1939
                     /locus_tag="PLU43_00605"
     CDS             866..1939
                     /locus_tag="PLU43_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO26935.1"
                     /translation="MNSLVHKIILILCCICFSVILNDSFDIVFPLLLVIIISSLTGYF
                     SNRILSTFLFVFYLILCCFFPLLIFFLPIVCYDIFLEKLQFIFGLSCIPFLVQYQYLN
                     GLNLAVILVLCMLSYILKHNYYNLKQFQNNYIKLRDETAEMANSLSKKNKELLEKQDY
                     EVNLATLNERNRIAREIHDNIGHMLSRSILQVGALLAITKDETTKEALEDIKTALTSG
                     MDSIRNSIHNIHEDSIDLKTKLEAIVHEFTFCSISFSYYVANDFTIKAKYSILYIVKE
                     ALNNVIKHSNATKVSISIDEHQNLYQIIISDNGTKKPEQGSSGSMGINSMQERIASLN
                     GTIHIDKDMGFRIFISLPKENKP"
     gene            1936..2556
                     /locus_tag="PLU43_00610"
     CDS             1936..2556
                     /locus_tag="PLU43_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO26936.1"
                     /translation="MNILIVDDDKLVCQALKTILETQEDIHVAAIGNDGTEAIDLYFR
                     HRPDILLMDIRMIAMTGIEAGKQIIAKDPLARILYLTTFEDNEYIIDALKLGAKGYLL
                     KQNFESIVPAIHAVYIGQRVFGDEIVNKLPDMMTSASKISFERYDLTEKEIEIITLIA
                     EGLSNREIAGNSFLSEGTVRNYISTILEKLQLRDRTQLAIFYYKNK"
     gene            complement(2635..2889)
                     /locus_tag="PLU43_00615"
     CDS             complement(2635..2889)
                     /locus_tag="PLU43_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO26937.1"
                     /translation="MTKKEVQIQLENGLEARPVALLVQVASQYESSIYIEVGDKKVNA
                     KSIMGMMTLILSAGDTVVVAAEGKDESAAVGGIESYLSGK"
     gene            complement(2936..3844)
                     /gene="whiA"
                     /locus_tag="PLU43_00620"
     CDS             complement(2936..3844)
                     /gene="whiA"
                     /locus_tag="PLU43_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOO26938.1"
                     /translation="MSFSGNVKEELGRHTSASRHCQIAELAAIYSFCKVETGNTKKDE
                     ILQFETENEITARKCFTLLKKTFNIYTNMCLNEQSLQKKGSTFQISIDQAEAADRIIQ
                     AVNNPVVTQKACCRRAYIRGAFLSAGSVSNPEKSYHFEIVCQNREQAILLQGLFHTFE
                     IEAKMIVRKKYFVVYIKEGSQIVEALNVMEAPVALMEFENLRILKEMRNSVNRRVNCE
                     TANIGKTVLAAVKQRSDIEAIIGTREYKELPDSVKAIAELRIRYPNASLKELGELSDP
                     PIGKSGVNHRLRKLSELAEKKQQSHN"
     gene            complement(3855..4730)
                     /gene="rapZ"
                     /locus_tag="PLU43_00625"
     CDS             complement(3855..4730)
                     /gene="rapZ"
                     /locus_tag="PLU43_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOO26939.1"
                     /translation="MRFVIVTGMSGAGKSTALKMFEDAGYYCVDNLPVPLIQKFSELV
                     SMPNSEIEKVALGVDVRTGQAFEELNHVLDHMKQAGFLFEVLFFEANDDVLIKRYKET
                     RRIHPLAGRDRVDQGIKREREKVASIKKRADYILDTSQMLTRELKAEIDRIFVNNQNY
                     KNLFITIVSFGFKYGIPTDADLVFDVRFLPNPYYFEELKYKNGNDKEVQDYVTGFPET
                     EVFLDKLEDMLIFLLPNYILEGKTQLVVTIGCTGGKHRSVTLANALYERMLKYLDYGF
                     KIEHRDIEKDSKRKK"
     gene            complement(4727..5650)
                     /gene="murB"
                     /locus_tag="PLU43_00630"
     CDS             complement(4727..5650)
                     /gene="murB"
                     /locus_tag="PLU43_00630"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOO26940.1"
                     /translation="MKQSVKEALNEILDGKNILWQEPMSAHTTFHVGGPADCFVTPVT
                     EQEVSAVLRLFSNEKEPFFILGNGSNLLVSDQGYRGTIIQFAGNYNEITIKGDRIIAQ
                     AGALLSEIAQRAYGQGLTGIEFASGIPGTLGGAAVMNAGAYDGEMKQVIEAVRLFDLQ
                     SGSIICKGNEEMKFGYRRSLLKEQPMLALEVTLKLQGGDPIKIKERMEELKASRAAKQ
                     PLEYPSAGSTFKRPEGYFAGKLIHDTGLQGYRVGGAMISEKHSGFVINAGDATAADVL
                     ALIRSVREKVYERFHVLLEPEVCMLGEDMTV"
     gene            complement(5675..>6353)
                     /locus_tag="PLU43_00635"
     CDS             complement(5675..>6353)
                     /locus_tag="PLU43_00635"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HOO26941.1"
                     /translation="NLGWPVMNLENELWELTKLPVKAGNDANVAALGEMWKGGGKGYD
                     DMVLVTLGTGVGGGIIVNGKILTGSNGAGGEIGHIHVEDEEAEACNCGNKGCLEQYTS
                     ATGIVRLANRKLAQTDAPSILRREEVTAKTVFDAVKENDTLAVEIAEEFGEYLGKGLA
                     AIAGVSNPDIFVIGGGVSKAGEILISYIQKYYKKYVFHASREAKFVLATLGNDAGIYG
                     AAKLVLD"
BASE COUNT         1668 a         1634 c         1124 g         1927 t
ORIGIN      
        1 tcacttcgtt attcaagcag ttaacataac ttacgcccgg catcttttca atttcttcca
       61 gtacagagga ggtgatccgg aatacttcta tcacgacctt ttcgtcttcc ccgacaagtg
      121 ctttcagttc ttcctcagac gctgtcagga taaccctgcc cttaaacagg atcgccattc
      181 tgctgcagat atgagccagc tcctcaatat catttgaagc atacaaaacc gttgttccct
      241 gtttattcag aacacggatc gcctgaagaa gcagctcttt tgtcatactg tccacaccgg
      301 ataccggctc gtccacaaca agaagctttg gtgccccggc aattccgcat gccatattca
      361 gccgttttaa aaacgaaaaa ttcaatttcg aaggcagcag tgtcctcact tcagaaagac
      421 cgcacatttt aactgcttct tccgcaagtt ccttctccct gctgcgctca tcaacataca
      481 gactgcagaa atacgcaata ttttcgtata cggtcagttc tttcagcacc gcaatttcct
      541 gtggaacaaa tccgatcttt ctttttaatt ccagattccc aggaaataac ttttccccgt
      601 caatcgtaat ttccccttta tcatacttta aaatacccat caggcagttc acaagtgtcg
      661 ttttccccga accgttcgtt ccgaacagtc caaagatctc cccctcctgt gcctcaacgc
      721 tcaaatggtc caatatgatc gtcctgtcgt atcgcttaac aagatttact atttttactg
      781 ccatgattcc ctccgatatg atatactaca aatataaagg aagcggcgcc gtcagacgcc
      841 aaacaattga atctgaggtt ttattatgaa tagcctggta cacaaaatca tccttatatt
      901 atgctgtatt tgtttttccg ttattttaaa cgacagtttt gacatcgtct ttccattact
      961 gcttgttatc attatcagtt ccctgacagg atatttttcg aaccggatac tgagtacatt
     1021 cctttttgtt ttttatttga ttctctgttg ttttttcccg ctgctgatct tttttctgcc
     1081 gatcgtctgc tatgatatct ttctggaaaa actgcagttt atttttggac tttcctgtat
     1141 tccgtttctt gttcagtatc aatatctaaa cggactgaac ttggctgtca ttcttgtcct
     1201 ttgtatgcta tcttatattt taaagcataa ctattataat ttaaagcaat ttcagaacaa
     1261 ttatatcaaa ttaagggatg aaacggcaga aatggccaac tccctcagca aaaaaaataa
     1321 ggagcttttg gagaagcagg actacgaagt caatctcgcc acattaaacg aacgcaatcg
     1381 tattgcaagg gaaatccacg acaatatcgg ccatatgctt tcccgttcta ttctgcaggt
     1441 aggcgctctt ctcgccataa caaaagatga aacgacaaag gaagccctgg aagacataaa
     1501 gactgccctt acttccggta tggacagtat ccggaacagt attcacaata ttcacgaaga
     1561 ttccatagac ctgaaaacaa agctggaggc gatcgtacac gaatttactt tctgcagtat
     1621 cagtttcagc tattacgtgg caaatgactt tacaataaaa gcaaaatact ctattctgta
     1681 tattgtaaag gaagccctga acaatgtaat caagcattcc aatgcgacca aagtctctat
     1741 ttccattgac gaacaccaga atctttatca gatcattata tctgataacg gaacaaaaaa
     1801 accggaacag ggatcttccg gttccatggg aatcaacagc atgcaggagc ggatcgcttc
     1861 cctgaacggt acgatacata ttgacaagga tatggggttc cgtattttta tttctttacc
     1921 aaaggagaat aaaccatgaa catattaatt gtggatgatg acaagcttgt atgccaggca
     1981 ttaaagacca ttttggaaac acaggaggat attcatgtcg cagctatcgg aaatgacggt
     2041 accgaggcca tagatcttta tttcaggcat agacccgata ttctgctgat ggatatccgt
     2101 atgatcgcca tgacaggtat cgaagccggc aaacagatca ttgcaaagga tccccttgcc
     2161 aggatcctgt acctgactac atttgaagac aacgaatata ttattgacgc tttaaagctc
     2221 ggggcaaaag gatatctgct taaacagaat tttgaaagca tcgttcctgc cattcatgcg
     2281 gtctatattg ggcagcgtgt gttcggtgat gaaatcgtca ataagcttcc ggatatgatg
     2341 acaagcgctt ccaaaatttc ttttgagcga tatgacctga ctgaaaagga aattgagatc
     2401 atcaccttga tcgcagaagg actgagcaat cgggaaattg ccggaaacag ctttttaagt
     2461 gaaggcaccg tacggaacta tatcagtacg atcctggaaa agcttcagct gcgtgaccgt
     2521 acccagcttg ctatctttta ttataagaat aaatagcttt cgcacataaa acaggacact
     2581 gccgcggcaa tcggcagtgt cctgttttat gttttccact atattaatga taaattattt
     2641 tccgctcaaa taactttcta tcccgccgac agctgccgat tcgtcttttc cttccgctgc
     2701 cacaacaacc gtatcgccgg ccgacaagat caatgtcatc atacccataa tacttttggc
     2761 atttactttt ttatctccga cctcaatata aatagaactt tcatattgac ttgccacctg
     2821 tacaagtagt gctaccggcc tcgcctcaag accattttct aactgaatct gtacttcctt
     2881 ttttgtcata atgcttgctc ctccttttat aatgcagcgt ttcttttttg acccttcaat
     2941 tatggctttg ctgtttcttt tctgccagct cgcttaattt acgcagcctg tgattaaccc
     3001 ctgattttcc tatcggtgga tcgctcagct caccaagttc ctttaaggag gcgttcggat
     3061 atcggatcct aagctctgcg atcgctttga cgctgtccgg cagttcttta tattccctcg
     3121 tgccgataat cgcctcaata tccgatctct gcttcactgc ggctaaaaca gtcttcccga
     3181 tatttgccgt ttcgcaattt actctgcggt ttaccgaatt tctcatctcc ttcaatatcc
     3241 gcagattttc aaactccatt aaagcaacgg gagcctccat cacgttcaat gcttccacga
     3301 tctgagaacc ctctttgata tatacgacaa aatacttctt gcgaacaatc atttttgctt
     3361 ctatctcaaa cgtatgaaaa agtccctgaa gaagaatcgc ctgctccctg ttttgacata
     3421 cgatctcaaa atgataagat ttttcaggat tgctgacaga gcccgccgat aaaaaagcgc
     3481 ctctgatata tgcgcgcctg cagcaggcct tttgagtcac gaccggattg tttaccgcct
     3541 gtatgatccg gtctgccgct tccgcctggt cgattgatat ctgaaacgta ctgccttttt
     3601 tctgcaatga ctgctcattt aagcacatat tcgtatatat attaaatgtt ttttttagta
     3661 atgtaaagca ttttctagcc gtgatctcat tttcggtctc gaactgcagg atttcatcct
     3721 tctttgtatt tcccgtttcc accttgcaaa aagaataaat tgcagccaat tccgcaatct
     3781 gacaatgcct ggatgcactc gtatgcctcc cgagttcttc cttgacattt ccggaaaaag
     3841 acataacagc acctctattt ctttcgtttg gaatctttct caatatctct gtgttctatt
     3901 ttaaaaccat agtccaaata tttcagcatc cgttcataca gtgcattcgc cagcgtaaca
     3961 cttcgatgct ttcctcctgt acagccgatc gtaacgacga gctgcgtctt tccctccaat
     4021 atataatttg gaagcaaaaa gataagcatg tcctccagct tatcaaggaa aacttctgtt
     4081 tccggaaatc ccgtcacata gtcctgcact tctttgtcat tcccattttt atatttcagc
     4141 tcttcaaaat aatacggatt cggaaggaaa cgtacgtcaa aaaccagatc cgcatcagtt
     4201 gggatcccat acttaaagcc aaaggaaacg attgtaataa acagattttt ataattctga
     4261 ttattcacaa agatcctgtc gatctcagcc ttcagctctc ttgtcagcat ctggctcgtg
     4321 tccaatatat aatccgcacg tttctttatg gacgctacct tttcccgttc tcttttgatt
     4381 ccctggtcca ccctgtcacg ccctgcaagc ggatggatcc tccttgtttc tttatatctt
     4441 ttgatcagta catcgtcgtt tgcctcaaaa aaaagtactt caaaaaggaa cccggcctgt
     4501 ttcatatggt ccaatacgtg attcagctct tcaaaggcct gtccggttct gacatcaacg
     4561 ccaagtgcca ccttttcgat ctcgctgttc ggcatggata caagctcgga aaacttctgt
     4621 atcagaggaa ccggcagatt gtccacgcag taatagccgg catcctcaaa cattttcaat
     4681 gccgtacttt ttcctgctcc tgacattccg gtaacgatta cgaatctcat actgtcatat
     4741 cctctccgag catacacact tccggctcca gcaaaacatg aaaccgctca tatacctttt
     4801 ccctgacact tctgatcaaa gcaagaacat ccgctgctgt tgcatcgcct gcattaatga
     4861 caaatccgct gtgcttttcc gatatcattg caccgccgac acgatatccc tgtaatccgg
     4921 tatcgtgaat cagtttcccg gcaaaatatc cttccggacg cttaaaagtg ctccctgcgc
     4981 ttggatattc caacggctgc ttcgcagcac gtgaagcttt cagttcttcc attctctctt
     5041 taatttttat cggatctccg ccctgcagct ttaatgtaac ctccagtgca agcattggct
     5101 gctcctttaa aaggctcctg cgataaccga atttcatctc ttcattgccc ttgcagataa
     5161 tactgccgct ttgcagatca aacaatctga ccgcttcgat gacctgtttc atttccccgt
     5221 cataagcacc ggcattcata acagctgccc cgccaagtgt tccgggaata cctgatgcaa
     5281 attcgattcc tgtcagtccc tgtccatacg ctctctgcgc gatctctgag agaagcgctc
     5341 ctgcctgtgc gataatacga tctcctttta tggtaatttc attataatta ccggcaaact
     5401 gaatgatcgt tccccgatag ccctgatcac tgactaaaag attacttccg tttcctaaaa
     5461 taaaaaacgg ttctttttca ttagaaaaaa ggcggagcac agccgatact tcctgttctg
     5521 taacgggggt cacaaagcaa tctgccggac caccgacatg aaaggtcgta tgcgcagaca
     5581 tgggctcctg ccataatata ttctttccgt ccagtatttc gttcaacgct tcctttaccg
     5641 actgtttcaa attaagactc cttatgatat ggcattaatc cagtacaagc tttgccgcgc
     5701 cataaattcc cgcatcattt ccgagtgtgg ccaacacaaa ttttgcttcc ctgctcgcat
     5761 ggaatacata ttttttataa tacttctgaa tataagaaat caaaatctct ccggcttttg
     5821 aaacaccgcc gccgattaca aaaatatcag gattgctgac ccctgcgatc gcagcaagac
     5881 cttttcccag atattctccg aattcttccg caatctcaac tgccagcgtg tcattttcct
     5941 tcactgcatc aaacacggtc ttcgcagtta cttcttccct gcggagtatg gacggcgcat
     6001 ctgtctgcgc aagcttcctg ttagccagac gcacgattcc cgttgccgac gtatactgct
     6061 caagacagcc tttatttccg caattacatg cttctgcttc ctcatcttcc acatgaatgt
     6121 ggccgatctc cccgccggca ccatttgacc ctgtcagtat ttttccattt acaataatac
     6181 cgccgccaac tcctgttcca agcgttacaa gcaccatatc gtcatagcct tttccgccgc
     6241 ctttccacat ttctccaaga gcggcgacat tggcatcgtt tcctgctttt acgggaagtt
     6301 ttgtgagttc ccaaagttca ttttccagat tcatgaccgg ccagccaaga tta
//
LOCUS       DAOSYE010000018         6257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_8208_length_6257_cov_3.560464, whole genome shotgun sequence.
ACCESSION   DAOSYE010000018 DAOSYE010000000
VERSION     DAOSYE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8208_length_6257_cov_3.560464"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLU43_00640"
     CDS             complement(<1..630)
                     /locus_tag="PLU43_00640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOO26942.1"
                     /translation="MKNAVQYQLVRAKRKTVSVCVKDDGTVEVRAPGWVSNARIRRFV
                     EEKSDWIIQKKMEIEQRNRAAGIRYTEGAVFTCLGTEYRLHIEESGKNAVLLQDDLLI
                     IKTRAADEASIKGQMIKWCGEQLACYIRLKAEEYLPLLSETAVWKQEKQTVITGITIR
                     NMKSRWGSCSAKGRLSFNVKLVLAPPKITDYVIIHELCHLQYMNHKKEFW"
     gene            complement(750..2774)
                     /locus_tag="PLU43_00645"
     CDS             complement(750..2774)
                     /locus_tag="PLU43_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOO26943.1"
                     /translation="MEKMYYGGDYNPEQWTPDIWEEDMRMFKLAGIDILTLNVFSWAL
                     LQPSEEEYDFSLLDRIVKLVTDSGMKICMATSTAAHPAWMAKRHPDICRVEWNGMKRK
                     FGGRHNSCPNSPTFQKYSVRLAEKLAERYRDQKNIVAWHVSNEYSGKCYCENCEKAFR
                     IWLQRKYGTLEKLNYEWNTAFWGHTFYDWDEIVAPSGLTEEFDQQCQYQERTTFQGIS
                     LDYMRFCSDSLIENYIAEYKAIRKHIPDAKITTNLMGTYKGMDYQKLGKYLDFASYDN
                     YPASTETPAFIGMKHDLMRGLKQGQPFWLMEQTPSVSNWLQNNSLKRPGVLRLWSYQA
                     VAHGADAVMYFQMRRSTGACEKYHGAVIDHVGNENTRVFREVSALGRELQQLGDEILG
                     SYLPKETAVIFDWDNWWATEYSAGPSTSLKYVDEIYRYYEALHGEHIPVDLISCEDRL
                     EDYKIVFAPLHYMVKPGVADRIKKYVEAGGRFVTTFFSGYVNENDHVTVGGYPGELRE
                     LLGIWVEEIDALPDERENHFSYNGVRYPAKLLCDLLHPEGANVRSSYEEDFYEGMPVL
                     TENSYGKGTAYYIATSSNEAFYHTYLKELCGEAGVIPLISSGTKQLEVTLREKPGVNY
                     YFFLNHSPQEIVFPAPAAGVDLLTEQGVKAEEAITLTGYGVMILKAAAQK"
     gene            complement(2960..3544)
                     /locus_tag="PLU43_00650"
     CDS             complement(2960..3544)
                     /locus_tag="PLU43_00650"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOO26944.1"
                     /translation="MAKLYFRHGAMGSSKTANALMVEYNYYERGKKALLLKPQIDSRD
                     GEKVIRSRMGLERPCNYVEDLVKMSEEEIRAYDCIIVDEAQFCKKSDIELFIHIVDDL
                     SIPVICYGLRTDFRREPFEGSIWLLAWADVIEELKTVCWCGQGASCNIRLNEKGEVVK
                     SGEQICLGANDKYMALCRRHYKEENLGPDFELPH"
     gene            complement(3562..5430)
                     /locus_tag="PLU43_00655"
     CDS             complement(3562..5430)
                     /locus_tag="PLU43_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOO26945.1"
                     /translation="MYCTRKVKDDLIWVGGNDRRLSLFEGVYSVPHGVSYNSYLLLDE
                     KTILFDTVDKAIGKTFFENLEYGLGGRPLDYVVVHHMEPDHSASLSELLLRYPDVKVV
                     CNEKTKTMIKNFFDKEVSVYLVKETDTLSTGKHSFRFYMAPMVHWPEVMVTYDETDKT
                     LFSADAFGTFGALNGALFADEVDFEHDYLDEARRYYANIIGKYGPQVQAFLKKADALD
                     IAMICPLHGFVWRENLEFYIEKYKKWSSYIPEETCVLMPYASIYGNTENIADIVSCRL
                     RDKGIQTVMFDVSVAPASEIIAAAFKYSHIVFASATYNAGIFVSMEELIRDLRAHNIQ
                     NRTIGFIQNGSWAPASGRLMKEILADCKDMKVLEQTITVISSAKKEQEEEIEAFVNAV
                     AETIPEADRSTGLPEKKTGVLDEKALFKLQYGLFVLTAKDGEKDNGCIVNTVQQVTSM
                     PNRITVAVNKQNYTHDMILKSGMFNVSVLTESAAFELFQNYGFRSGRDCDKFEGIETV
                     RRSANGLLYLTKNTDAVLSGKVVGSYDYGTHTIFIAEVTEAAILSEEPSVTYTYYFEH
                     IKPKPEKKTSKGKVWVCVICGYQYDEEKEGTPFEELPADWLCPICKHPKSDFVLQE"
     gene            complement(5466..>6257)
                     /locus_tag="PLU43_00660"
     CDS             complement(5466..>6257)
                     /locus_tag="PLU43_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO26946.1"
                     /translation="AILEADQSDETGIFEQRKRVEELKKKLSSDNSGEAKQLLSLADM
                     LVKKSVWIIGGDGWAYDIGFSGLDHIFASGEDVNILVLDTEVYSNTGGQASKSTNTGA
                     SAEFAISGKTGKKKSLAGIAMSYEDVYVAQVSMGANNAQCVKAFSEAESYHGTSIVIA
                     YAPCILHGIAGGMTYSQTEEKKAVESGYWNLFRFDPRLREEGKNAFVLDSKDPQLDYQ
                     EFLKGEVRFNALARSNPEHAKELFEASEKYANHRLENLKALAAEK"
BASE COUNT         1535 a         1676 c         1156 g         1890 t
ORIGIN      
        1 ccaaaattct ttcttatgat tcatgtattg caagtgacac agctcgtgta tgattacata
       61 atccgttatt ttaggtggcg ccagcacaag ttttacatta aaactgagac ggcctttggc
      121 agagcagctg ccccagcggc ttttcatatt gcgtatggta ataccggtaa tcacagtctg
      181 cttctcttgt ttccaaacag ccgtctctga cagaagcggc agatattcct ccgctttcag
      241 ccgtatataa caggccagct gctccccgca ccatttgatc atttgtcctt ttatagacgc
      301 ttcgtccgca gcacgcgttt ttataataag aagatcgtcc tgtaaaagca ctgcattttt
      361 cccgctttct tcaatatgca gcctatattc ggttccaaga catgtaaaaa cagcaccttc
      421 cgtatatcgg attcctgcag cccgatttct ttgctcgatt tccattttct tttgtatgat
      481 ccagtctgat ttttcttcaa caaagcgccg gatccgggca tttgacaccc agcccggggc
      541 acgcacttcc actgttccgt catcttttac acaaaccgaa actgtttttc gctttgcgcg
      601 gaccaattga tactgtactg catttttcat cctttttcct ttcctgcttt ttcatttttg
      661 cctggactgc aaaaacagca gaaagcaccg cctcatgacc ggatatagtc agtggaagtg
      721 ctcttgtatt ttctataatt atagtttatt tatttctgtg ctgcggcttt cagtatcatg
      781 acaccatagc cggtaagtgt aatggcttcc tctgctttta caccctgttc tgtcagaagg
      841 tccacccctg ctgccggtgc aggaaacacg atttcctgcg gcgaatgatt cagaaagaag
      901 taataattca cgcccggctt ttcccgcagg gtgacttcca gctgttttgt cccgctgcta
      961 atcagcggta ttacacctgc ctcaccgcaa agctccttca ggtacgtgtg ataaaaggct
     1021 tcgtttgatg aggtcgctat gtaatatgcc gttccctttc cataggagtt ttccgtgagc
     1081 accggcattc cctcataaaa atcttcttca taactgctgc gcacattcgc tccttccgga
     1141 tgcaaaagat cacagagcag ttttgcaggg tatctgacgc cgttatagct gaaatgattt
     1201 tccctttcat caggaagagc atcgatctcc tctacccaga ttccaagcag ttctcgcagc
     1261 tcgcccggat aaccgcccac ggtaacatgg tcattttcat tcacatagcc gctgaagaaa
     1321 gtcgttacaa aacggcctcc ggcttccacg tattttttga tgcggtcggc aacgcccggc
     1381 tttaccatat aatgcagcgg agcaaaaaca atcttataat cctccagcct gtcctcacag
     1441 gaaatcaggt ccaccggaat atgctccccg tgcagtgctt cataataacg gtatatctca
     1501 tccacgtatt ttaaggaagt gctcgggccg gcagaatact ctgtcgccca ccagttatcc
     1561 cagtcaaaaa taacagctgt ttcttttgga agataacttc ccaatatttc atctcccagc
     1621 tgctgcagtt cccttcccaa ggcagaaacc tcacggaata cccttgtgtt ttcgtttccc
     1681 acatgatcaa tgacagcccc atggtatttt tcacaggctc cggtactgcg ccgcatctga
     1741 aaatacatca ctgcgtccgc tccgtgcgct acagcctgat agctccagag ccgaagtact
     1801 ccagggcgct tcagactgtt attttgcagc cagttggata cacttggcgt ctgttccatc
     1861 agccaaaagg gctgtccctg cttcagaccg cgcatgagat catgcttcat tcctataaaa
     1921 gccggtgttt ccgtactggc cggataattg tcataagagg caaaatccag atatttgccc
     1981 agtttctggt aatccatgcc tttataggtt cccatcagat tcgtggtaat ttttgcatcc
     2041 ggaatgtgct tcctgattgc tttatattcc gctatataat tttcaatcag ggaatcgctg
     2101 cagaatctca tataatcaag ggagatcccc tgaaaggtcg tacgttcctg atactggcac
     2161 tgctggtcaa actcctcagt cagtccgctc ggcgcaacga tctcatccca gtcataaaac
     2221 gtatgtcccc aaaatgccgt attccactca taattcagtt tttccagcgt gccatacttt
     2281 ctctgaagcc agatccgaaa ggctttctca caattttcac aataacattt tccgctgtac
     2341 tcattgctca catgccatgc cacaatattt ttctgatcac gatatctttc cgcaagcttc
     2401 tccgcaagcc ttacggaata tttctgaaag gtcgggctgt tcggacagga attatgacgt
     2461 ccgccgaatt ttcgtttcat gccgttccac tccacccggc agatatccgg gtgacgcttt
     2521 gccatccatg ccggatgtgc cgctgtggaa gtggccatac agatcttcat tccactatct
     2581 gtgaccagct tcacgatccg gtctaacagc gaaaaatcat actcctcctc actgggctga
     2641 agcagtgccc agctgaacac attcagtgtc aaaatatcga ttccggccag cttgaacatc
     2701 cgcatatcct cttcccagat atccggtgtc cactgctccg gattgtaatc tcccccataa
     2761 tacattttct ccatgtcaaa tacctctctg cataaaattt ttgaagtctt ctctatttta
     2821 tcatagatat ctgccgatat ctatttaaca tttgcataaa taatatttct gatcctcctt
     2881 gtatcacaaa aaaacactga cagcagtata tggataaata ccagtcagtg tttttatgat
     2941 ccggatactg ccattccgat taatgcggca gctcaaaatc cggtcccaga ttttcttctt
     3001 tataatgcct gcggcaaagc gccatatatt tatcatttgc gccaagacag atctgctcac
     3061 cgcttttcac gacttctcct ttttcgttca gacggatatt gcagcttgct ccctgcccgc
     3121 accagcagac tgtttttaat tcctcgatca catcggccca ggccagaagc cagatagacc
     3181 cttcaaacgg ctctcgccga aaatccgtac gcaggccata acagatcacc ggaatactta
     3241 aatcatcaac aatatgaata aacagttcta tgtcggattt tttgcaaaac tgcgcttcgt
     3301 caacaataat acagtcatat gccctgattt cttcctcact catttttaca aggtcttcga
     3361 catagttaca cggccgctca agtcccatac ggcttctgat cactttttcg ccgtccctgc
     3421 tgtcgatctg cggtttcaat aacagtgctt tcttcccgcg ttcataataa ttatactcga
     3481 ccatcagtgc gtttgccgtc ttggaacttc ccattgctcc atgcctgaaa taaagctttg
     3541 ccatatcgtt gtctctcttt tctattcctg aagaacaaaa tccgattttg gatgcttgca
     3601 gatcggacat aaccagtctg ccggcagctc ttcaaacggt gtgccttctt tttcttcgtc
     3661 gtactgatac ccgcatatga cacataccca gacttttcct ttggaagtct tcttttcagg
     3721 ctttggttta atatgctcaa aataatatgt gtacgttacg gaaggttctt cggacaaaat
     3781 ggccgcttct gttacttccg caataaatat cgtatgcgtt ccgtaatcat aagaaccaac
     3841 tactttaccc gataacaccg catcggtatt cttcgtcaga tacagcaggc cgttagccga
     3901 ccgccgtact gtttctattc cttcaaactt atcacaatca cggccgctac ggaaaccata
     3961 gttctgaaac agttcaaacg cagcgctttc agtcagcacc gacacattga acatgccgct
     4021 ctttaagatc atgtcatgcg tataattctg tttgttgacc gcaacggtga tgcggttcgg
     4081 catactcgtt acctgctgga ccgtattgac aatacagccg ttatcttttt ctccgtcttt
     4141 tgccgtcagc acaaacagac cgtactgcag cttgaacagc gccttttcat ccagcactcc
     4201 cgtcttcttt tccggcagtc ccgtacttct gtcggcctca ggtatcgttt ctgccactgc
     4261 atttacaaac gcctcgattt cttcctcctg ctcctttttc gcagaagaaa tgactgtgat
     4321 cgtctgctct aaaactttca tatccttgca gtccgcaaga atttctttca ttaatctgcc
     4381 gcttgccggt gcccaggaac cattctggat aaatccgatc gtgcggttct ggatattatg
     4441 agctcttaaa tccctgatca gttcttccat gctcacaaaa attccggcat tgtatgttgc
     4501 agaagcaaat acaatatgac tgtacttaaa tgccgctgca ataatttcag aggccggggc
     4561 taccgaaaca tcaaacatca ctgtttggat ccccttatct ctcagtctgc acgaaacaat
     4621 atccgcgata ttttccgtat ttccataaat agaagcataa ggcatcagca cgcacgtttc
     4681 ttcgggaatg taactgctcc attttttata tttttcaata taaaattcca ggttttctct
     4741 ccatacaaat ccatggagag gacagatcat tgcaatgtcc agtgcatccg cttttttcag
     4801 aaatgcctgc acctgcggtc cgtatttccc tataatattt gcataataac ggcgtgcttc
     4861 atccagatag tcatgctcaa agtccacttc atcggcaaac agtgcaccgt tgagtgcacc
     4921 gaacgtacca aacgcatctg ccgaaaaaag tgttttatcc gtttcatcgt atgtaaccat
     4981 gacctccggc cagtgcacca taggtgccat ataaaaccgg aagctgtgtt ttcccgtact
     5041 cagtgtatcc gtttctttta caagatacac tgagacttct ttatcaaaga agttctttat
     5101 catcgttttt gttttttcgt tacatacgac ttttacgtcc ggatagcgca gaagtaattc
     5161 actcaggctc gcggaatgat ccggctccat atgatgaacg accacataat ccaaaggtct
     5221 tccgcccaaa ccatattcca ggttttcaaa aaaggtcttt ccaattgctt tatccaccgt
     5281 atcaaaaaga atcgtctttt cgtcaagcag cagataggaa ttataggaaa ccccatgcgg
     5341 tacggaatat actccttcaa acagagacag tctgcgatca tttcctccta cccatatcaa
     5401 gtcgtctttt actttccttg tgcaatacat gactttcctc cttatgcttc ttaatcattc
     5461 aggttttatt tctctgctgc cagcgctttc agattctcaa gtctgtgatt tgcatatttc
     5521 tccgaagctt caaaaagctc ttttgcatgt tccggattgg aacgagccag tgcgttgaat
     5581 cgtacttcgc cctttaagaa ttcctgatag tcaagctgcg gatctttcga atccagtaca
     5641 aacgcattct tgccttcttc tctcagtctc gggtcaaaac ggaacagatt ccagtatccg
     5701 ctctctactg ctttcttctc ctctgtctga gaatatgtca ttcctcctgc aattccatga
     5761 aggatacaag gtgcatatgc aattacaata gaagttccgt gatagctttc ggcttcgctg
     5821 aatgccttga cgcactgtgc gttgttagca cccatggaga cctgggccac ataaacatct
     5881 tcatagctca tggcaattcc tgcaaggctc ttcttcttgc cggtttttcc gctgattgca
     5941 aattcagccg acgcgccggt atttgttgac ttggaagcct gtccgcctgt attggagtaa
     6001 acttccgtat ccagtacgag aatatttaca tcttcgcccg atgcaaaaat atgatccagg
     6061 cctgagaaac cgatatcata agcccagccg tcaccgccga tgatccagac gcttttctta
     6121 acaagcatat cagcaagact taaaagctgt ttggcttcgc ctgaattatc tgaagataat
     6181 tttttcttta attcctcaac tctttttctc tgctcaaaaa tcccggtttc atcagactgg
     6241 tcagcttcca atatagc
//
LOCUS       DAOSYE010000019         6154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_8403_length_6154_cov_2.844073, whole genome shotgun sequence.
ACCESSION   DAOSYE010000019 DAOSYE010000000
VERSION     DAOSYE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8403_length_6154_cov_2.844073"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLU43_00665"
     CDS             <1..1197
                     /locus_tag="PLU43_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOO26947.1"
                     /translation="PGGIIVLPIGEEIESFTPIQHPANDMTTDIVTTHFDYHSIDHNL
                     LKLDILGHDDPTMIRRLQDLTGTDPQRVPFDDKEVMSLFQNTKALGILPSDIGGCPLG
                     SLGIPEFGTDFAMQMLLDTHPTSFSDLVRIAGLAHGTDVWLGNAQTLIQDGTATISTA
                     ICTRDDIMTYLIGMGMESGLSFTIMEAVRKGKGLKDDWETAMQEAGVPDWYIWSCNKI
                     KYMFPKAHAAAYVMMAWRIAYYKINYPLAYYAAFFSIRATGFSYEIMCQGQERLAYYL
                     ADYKKRSDSLTKKEQDVMRDMRIVQEMYARGYEFIPLDLFRAKAHECQIIDGRIMPFI
                     GSIEGLGDKAADAVVEAVKDGPFLSKDDFRERTKVSKTVIETMDSFGLLGSLPESNQL
                     SLFDFG"
     gene            1284..1355
                     /locus_tag="PLU43_00670"
     tRNA            1284..1355
                     /locus_tag="PLU43_00670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1317..1319,aa:Glu,seq:ctc)
     gene            1608..2072
                     /gene="rimP"
                     /locus_tag="PLU43_00675"
     CDS             1608..2072
                     /gene="rimP"
                     /locus_tag="PLU43_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOO26948.1"
                     /translation="MSRREEYERRTEELILPIIEEYSFELWDVEYVKEGSDWYLRVYI
                     DKPGGITIDDCVLVSRRLSDQLDKEDYMEDAYILEVSSPGLGRQLKKDKDLKRCIGQC
                     VDLKTYRMMDGRKDFTGILRAFDKESITIEINESNVSFQRGDVALVKLSIDF"
     gene            2100..3257
                     /gene="nusA"
                     /locus_tag="PLU43_00680"
     CDS             2100..3257
                     /gene="nusA"
                     /locus_tag="PLU43_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOO26949.1"
                     /translation="MNKELMEALDILEREKDISKETLFDAIENSLLTACKNHFGKADN
                     VKVEIDRETCDFRVFAEKTAVIEPEDPVMEISLADAKEIDPDVKEGDVIEVDIKSKEF
                     GRIATQNAKNVILQKIREEERTVLYNQYYGKEKDVVTGIVQRYVGRNISIDLGKADAL
                     LAENEQVKNENLKPTERVKVYILEVKDTTKGPKILVSRTHPELVKRLFESEVTEVHDG
                     TVEIKAIAREAGSRTKIAVWSNNPDVDPVGACVGMNGTRVNAIVDELHGEKIDIINWD
                     ENPGILIENALSPAKVVFVIADGDEKTAKVVVPDTQLSLAIGKEGQNARLAARLTGFK
                     IDIKSETQAKDAPGFRLEDYYDDEEEYDEDLYEEESYEDEYTEDEKAAQTE"
     gene            3291..3560
                     /locus_tag="PLU43_00685"
     CDS             3291..3560
                     /locus_tag="PLU43_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOO26950.1"
                     /translation="MAKKVPLRQCVGCGEMKSKKEMMRVIKTADDEIVLDKTGKKNCR
                     GAYLCMNKECLERAVKQKGLERSLKTAIPEEVVESLKKEFDGSEA"
     gene            3544..3867
                     /locus_tag="PLU43_00690"
     CDS             3544..3867
                     /locus_tag="PLU43_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOO26951.1"
                     /translation="MEVKPDKVLQLVGMAQRAHYAAGGEFMTETMVKSKKASVVIIAE
                     DASDNTKKHFFDMCRYYKVPCFVYGTKESLGHAIGKEMRASLSVTNEGLAKEIISKID
                     GGSKE"
     gene            3864..>6154
                     /gene="infB"
                     /locus_tag="PLU43_00695"
     CDS             3864..>6154
                     /gene="infB"
                     /locus_tag="PLU43_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO26952.1"
                     /translation="MRVHELAKKIDRTSKDVLTCLEQAGIMGKTASSSLEEAQIAVVR
                     KKFPSAPPAGKEPVKAEEKPPEKEKPKAADALPKKKKSNIIFVSNPHNSQVQGRNNAG
                     FGQRVVKGTPVRPGSQQSNAKPQKLPPRSSDAVQSSDRTPGSTQSLGGDQNKQIRTQS
                     QENVKNNKTNVQRSGSSVRTGNYNNYDNQRNEGYRNQTERVRQPYQGNRDTQRQGQYQ
                     NRDGQNRGSYQNRDGQNRGSYQNRDGQGQNRGSYQNRDGQNRGPYQNRDGQNRGPYQN
                     RDGQNRGPYQNRDGQNSRPGQPYAQGNRNFQNNRGNVNGGFSKDAKESVKEQEKHRDE
                     QEKRRQNQDKRKKEQTYKNEDGFRGGRTAKGTKFTKLGRPQEKQVDIVKEEEKIKVII
                     LPEVMTIRELADKMKIQPAVIVKKLFLQGKVVVVNQEITYEQAEEIAVEFDVLCEKEV
                     VVDVIEELLKEDEEDQSEMTSRPPVICVMGHVDHGKTSLLDAIRKTHVIDKEAGGITQ
                     HIGAYVVDVNGRRITFLDTPGHEAFTAMRMRGANSTDIAILVVAADDGVMPQTIEAIN
                     HAKAAGIEIIVAVNKIDKPSANIDRVKQELSEYELVPEDWGGSTVYASVSAKTGEGIE
                     NLLEMILLSSDILELKANANRRARGLVIEAQLDKGRGPVATVLVQKGTLSVGDFIAAG
                     SCHGKVRAMIDDKGKRVKEAGPSTPVEILGLNDVPNAGEIFVSPENDKEARNFAATFI
                     SQNKVKLLDDTKAKMSLDDLFTQ"
BASE COUNT         1934 a         1083 c         1688 g         1449 t
ORIGIN      
        1 cccggcggaa tcattgtgct gccgatcgga gaggagatcg aatcgttcac accgatccag
       61 catccggcca atgacatgac aactgatatt gttacgacgc attttgatta tcattccatt
      121 gaccataatc tgctgaagct ggatatactg ggacatgacg atccgacaat gatccggcgt
      181 ctgcaggatc tgacaggaac cgatccgcag cgtgtgcctt ttgatgataa ggaagtaatg
      241 tcactgtttc aaaatacaaa ggcgcttggc attctgccgt ccgatattgg cggctgtccg
      301 cttgggtctc tcgggatccc ggaattcgga acggattttg ccatgcagat gttactggat
      361 acgcatccga cttcgttttc cgatcttgtg cggatcgccg gccttgccca tggtacggat
      421 gtatggcttg gcaatgctca gacattgatt caggacggaa cggcgacgat ttccactgct
      481 atttgtacgc gtgatgatat tatgacatat ctgatcggta tgggaatgga gagcggactt
      541 tcctttacaa ttatggaagc agtgcgaaaa gggaaagggc tgaaggatga ctgggaaacg
      601 gccatgcagg aggcaggtgt gccagattgg tatatctggt cgtgcaataa gatcaaatac
      661 atgtttccca aagcgcatgc agcagcctat gttatgatgg catggaggat cgcctattat
      721 aaaatcaatt atccgctggc atattatgca gcgtttttca gtatccgtgc gacaggattt
      781 tcgtatgaga tcatgtgcca gggacaggaa cggcttgcgt attatctggc cgattacaaa
      841 aagcgttccg acagccttac gaaaaaggag caggatgtta tgcgcgatat gcgcattgta
      901 caggaaatgt atgcaagagg ttatgagttt ataccacttg atctgttccg ggcgaaagca
      961 catgaatgcc agatcataga cggcaggatc atgcccttta tcggcagcat tgaagggctt
     1021 ggggataaag cggcggacgc ggttgtggaa gctgtaaagg acggcccgtt tctttccaag
     1081 gatgattttc gcgaacgcac aaaggtcagc aagaccgtta ttgaaacaat ggatagtttt
     1141 gggctgcttg gctctcttcc ggaatccaat cagttgtccc tgtttgattt tggctgaact
     1201 ccatatgcaa ttaaaaatcc agttaatatt tttataaaaa gtacttgcat ttgcaaataa
     1261 aataatataa aattagcaag tacggctcgt tggtcaagcg gttaagacgc cgccctctca
     1321 cggcggaaac aggggttcga ttcccctacg agctgttgac tgttattaaa cagcccaacc
     1381 cgaaaagttc ttggcaccgg cagctatgct ggtttcagga attttttatt tgatgaattt
     1441 ttgcttgcat ttttccttat gtagtggtat tattacgttg attgatactg gatgataaga
     1501 gtggacttgc ggtccactct tattatgtgt gacggcaatt ttaataaaag cagatacaga
     1561 gcagtaattt gaagagtatg gagctgctgt tataaggagg aagacagttg tcaagaagag
     1621 aagaatatga acgcaggaca gaagaactga tcttgccgat cattgaggaa tattcttttg
     1681 agttgtggga tgttgaatat gtaaaagaag gttctgactg gtatttacgg gtttatatag
     1741 ataaaccggg cggtattacg atagatgact gcgtgcttgt cagccgccgg ctttccgatc
     1801 agctggataa agaagattat atggaagatg cttatattct ggaggtcagt tcgccggggc
     1861 tcggaagaca gcttaagaaa gacaaggatc tgaaaagatg tatcggccag tgtgttgatt
     1921 taaaaacata tcgtatgatg gatggcagaa aagatttcac gggcatatta agagcctttg
     1981 ataaagaaag tattacaatt gagatcaatg aaagcaatgt cagcttccaa agaggggatg
     2041 ttgcactcgt aaaattatcg attgattttt aattctattc aaacttagga ggtcaggaaa
     2101 tgaacaagga attaatggaa gcgcttgata ttctggagag agaaaaagat atcagcaaag
     2161 agacattatt tgatgcgatt gaaaattcgc tgctgacagc atgcaaaaat cattttggca
     2221 aagcggataa tgtaaaagta gaaattgaca gggaaacatg tgattttcgt gtgtttgcgg
     2281 aaaagactgc tgtaattgag ccggaggatc cggtaatgga aatcagtctt gcggatgcaa
     2341 aagagatcga tccggatgta aaagaaggtg atgtgatcga agttgacatc aaatcaaagg
     2401 aattcggacg tattgcgaca cagaatgcaa aaaacgttat tctgcagaag attcgtgaag
     2461 aagaaagaac ggttctatat aatcagtatt atggaaaaga gaaagatgtt gtgacaggta
     2521 tcgtgcagcg gtatgtagga agaaatatca gtattgatct tggaaaggct gatgcgctgc
     2581 ttgctgaaaa tgagcaggtg aaaaatgaga acctgaagcc gaccgaaagg gtaaaagtat
     2641 acattcttga agtgaaagat acgaccaagg gtcctaaaat tcttgtcagc cgtacgcatc
     2701 cggagcttgt gaagcgtctg ttcgaatcgg aagtgacaga ggtccatgac ggcacggtgg
     2761 aaatcaaagc aattgcgaga gaagcaggga gcaggacaaa aattgctgtc tggtctaaca
     2821 atccggatgt tgatccggtc ggtgcctgtg taggtatgaa cggtacaagg gtcaacgcaa
     2881 ttgtggatga actgcatggt gagaaaatag atattattaa ctgggatgag aatcctggta
     2941 ttttgattga aaatgcattg tctccggcca aggtcgtttt cgtgatcgct gatggagatg
     3001 aaaagacggc taaggtcgtt gttccggata cgcagctttc gcttgcgatc ggcaaggaag
     3061 ggcagaacgc acggcttgct gcgagactga ccgggtttaa gatcgacatc aagagcgaga
     3121 cgcaggcaaa agatgcgcct ggattcaggc tggaagatta ttatgatgat gaagaagaat
     3181 atgatgaaga tttatatgag gaggaaagct acgaagacga atatacggaa gatgaaaaag
     3241 ccgcgcagac ggaatagcag gatgattttt tatggaagag aggtgtttta atggcaaaaa
     3301 aggttccgct cagacaatgt gtcggttgtg gtgagatgaa aagcaaaaag gaaatgatgc
     3361 gtgtaatcaa aacggcagac gatgaaattg tcttggataa aacgggaaag aagaattgca
     3421 gaggtgccta cctgtgtatg aacaaggaat gtcttgaacg ggctgtgaaa cagaagggac
     3481 tggaacgctc attaaaaact gcgattcccg aggaagtcgt tgagagtctg aaaaaggagt
     3541 ttgatggaag tgaagcctga taaagtattg cagcttgtcg gaatggcgca gcgcgctcat
     3601 tatgcggcag gcggagaatt catgacagag actatggtaa aatcaaaaaa agcgtcagtg
     3661 gtgattatag cggaagatgc ttcggataat acgaaaaaac atttttttga tatgtgcaga
     3721 tattataaag ttccatgctt tgtttatgga acaaaggaaa gtctcgggca tgcaatcgga
     3781 aaagaaatgc gggcatcgct ttcggtaacg aatgagggct tggcaaaaga gattataagt
     3841 aaaatcgacg gaggtagcaa agaatgagag tacatgagtt ggcaaagaaa atagatcgaa
     3901 caagtaaaga tgttcttacc tgtctggaac aggccgggat catggggaaa acggcgtctt
     3961 cttcattaga ggaagcgcag atagcagttg tcaggaagaa attcccctct gcaccgccgg
     4021 caggaaaaga accggtaaaa gcggaagaaa aaccgccgga aaaggagaaa ccaaaggcag
     4081 cggatgcgtt acccaaaaag aagaaatcaa atatcatttt tgtaagcaat ccgcataata
     4141 gtcaggtgca gggcagaaat aatgcaggat tcgggcagcg tgtagtaaag ggaacgcccg
     4201 taagacctgg gagccagcag agtaatgcaa agccgcagaa gctgccgccc agatcttcag
     4261 atgctgtcca gtcttcagac aggacgccag gaagcacgca gagtttggga ggcgatcaaa
     4321 ataaacagat tcgtacacaa agtcaagaaa atgttaagaa caataaaacg aacgtacaaa
     4381 ggagcggcag ttctgtcagg actggtaatt ataataatta cgataatcag cgaaatgaag
     4441 gatatcgaaa ccagacggaa cgggtaagac agccgtatca gggtaaccgt gatacacagc
     4501 gtcagggtca gtatcagaat cgtgacggcc agaacagagg atcctatcag aatcgtgacg
     4561 gtcagaacag gggctcctac caaaatcgtg acggccaggg ccagaacagg ggttcctacc
     4621 agaatcgtga tggtcagaac aggggacctt accagaatcg tgatggtcag aacaggggac
     4681 cttaccagaa tcgtgacggt cagaacaggg gaccctatca aaaccgtgac ggccagaaca
     4741 gcaggccggg gcagccttat gcacagggaa acagaaattt ccagaataac cgcggaaatg
     4801 ttaacggagg attttccaaa gatgcgaaag aaagtgtaaa ggaacaggag aagcatcgcg
     4861 acgaacagga aaagcgcaga caaaatcagg ataagcgtaa aaaagaacag acttataaaa
     4921 atgaggacgg tttcagaggc ggaagaacgg ccaaaggtac gaagtttaca aaattgggaa
     4981 ggccgcagga aaaacaggtt gatatagtaa aagaagaaga aaaaatcaaa gtaattattc
     5041 ttcctgaagt aatgacgatc agagaacttg cggataagat gaagatccag ccggctgtta
     5101 ttgttaaaaa gctgtttttg cagggcaagg tcgttgttgt gaaccaggag atcacctatg
     5161 agcaggcaga agaaattgcc gtggaatttg acgtcctctg tgaaaaagaa gttgttgtcg
     5221 atgtaataga ggagcttttg aaggaagatg aggaggatca gtctgaaatg acatcccgtc
     5281 cgcctgttat ttgtgtgatg ggtcacgttg atcatggaaa gacgtcgctt ctggacgcga
     5341 tcagaaaaac ccatgtgatt gacaaggaag ccggcggcat tacacagcat attggtgctt
     5401 atgtggtaga tgtaaacggc cgcaggatca catttcttga tacgccggga catgaagcgt
     5461 ttacagccat gcgtatgagg ggagctaatt ctacggatat tgccattctg gttgtggctg
     5521 ctgatgacgg tgtgatgccg cagacaatag aagcgatcaa tcatgccaag gctgccggaa
     5581 tcgagattat tgtggcagtt aataaaattg ataaaccaag tgcgaatatt gacagagtga
     5641 agcaggaact ttccgaatat gagcttgttc cggaagactg gggcggcagt acggtctatg
     5701 cgtcggtatc ggcaaagaca ggagaaggta ttgaaaatct tctggagatg atattgttat
     5761 cttccgatat tcttgaactg aaagcaaacg caaacaggcg tgcaagaggt cttgtgatcg
     5821 aagcacagct ggataaagga cgcggacctg ttgcaacggt tcttgtgcag aaaggaacat
     5881 taagcgtcgg tgactttatc gcggccggct catgtcacgg taaagtacgt gcaatgattg
     5941 acgataaagg aaaacgtgta aaagaagcgg gaccttccac gcctgtagag attctcggtc
     6001 tgaatgacgt gccgaatgcg ggtgagattt ttgtatcccc ggagaacgac aaggaggcaa
     6061 ggaattttgc agctacgttc atatcgcaga acaaagtaaa gctgcttgat gatacgaagg
     6121 caaaaatgtc tcttgacgat ctgtttacgc agat
//
LOCUS       DAOSYE010000020         6093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_8530_length_6093_cov_3.265485, whole genome shotgun sequence.
ACCESSION   DAOSYE010000020 DAOSYE010000000
VERSION     DAOSYE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_6093_cov_3.265485"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PLU43_00700"
     CDS             <1..1173
                     /locus_tag="PLU43_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO26953.1"
                     /translation="GLGSLRISTFTFAIIYLVVGIIFSFILSTLISRPIIALTKKLDI
                     IAEGDFSQEVEGNLIKIKDETGMLARAIEKMRDSVSSVIKTVMNESKAVFENVSSQGE
                     KVSHLQAQIEDVSATTEQLSAGMQETAASSEEMNTVMNEIEESVESIALRTQEGSKTV
                     NEIYERAKIFKEKAVKSKEIAYNIYETSSEHLQDAIRQSQDVEQINTLSESVLQITAQ
                     TNLLALNAAIEAARAGEAGKGFAVVADEIGRLAESSKNSVAEIQKVTKTVVASVENLS
                     ENSMQVLDFIKDKVMDDYDSLVEVSEKYHSDADTVDNIMKDFSAKTEELTSSIDKIMN
                     SISGITIATNEGAEGTTSIAQNVVAVSNAAKEVVDYASDTKNNATKLVEAVEVFKV"
     gene            1324..2568
                     /locus_tag="PLU43_00705"
     CDS             1324..2568
                     /locus_tag="PLU43_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO26954.1"
                     /translation="MKLTKEEKSWIMYDCGNSAYSMAVTTALLPIVFGMFQNVESSMD
                     LGYFNSIASLIVALLSPIFGTIADYKDKKKRFFLFFAVLGIFTTAALALVSPSGGKWQ
                     ILILFFILSVIGFNGSCIFYDAFLPDVTSDDRMDGISSRGFAFGYIFSVIPFGISLLI
                     IFLLGMDQAAGYQIGFVITALWWGLFTIPMIRNVKQRYYIEPEPAPVKNSFIRLFRTL
                     KNIIQYKYVFIFLLAYFFYIDGVDTIIKMVVPYATAILGGNAVSTFTLLGILLIIQIV
                     AFPCAILYGNLAKKYSARVMIIAGIFTYIVACIAAFFISSVWHIFILGVLIGSAQGGI
                     QALSRSYFAKIIPKERSNEFFGFYNVFGKFSAIIGPFLMALTTNMTGNARYSILSIIP
                     LFIIGFFVFLTLPKSAAAVIKE"
     gene            2574..3905
                     /locus_tag="PLU43_00710"
     CDS             2574..3905
                     /locus_tag="PLU43_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOO26955.1"
                     /translation="MKKNVNQKKYLIVISYDAFSEDNWEFASKLPHLSSLIGRGVHST
                     DLCSIYPTLTYAVHTTMVTGEYPDVHGVIHNNPFQPFVEENDQRWYWYRNEVKAPTVY
                     DVCREHGLTTAGLLWPVTGKSSIQYNIPEVKAIGKENQIIKMLRSGSPVFSILMELKH
                     GKTRKGISQPYLDDFTAQCCADTVVKKQPNVMMLHFTDLDTQKHDFGTKSRQVEEAMI
                     RMDARIGLILDAIKRSGNEASTTVMILGDHGQMDVRYQIRLNAAFYREGLIYKEQGTL
                     NWRAYVQGADGCAYLYVRAHDEEAKEKAFAIIHEMMKEKNSGIELLFTGEDIREKHLV
                     PSMEAVLEARNGFYFNDSIEGPVIFDLEKAGLRHATHGYLPQKPGYKCCFLMAGNGIR
                     QGQELKGIHMTDIAPTMAAVMGVEFPYGKGRILKVFTAEDSNTNPEHRKST"
     gene            complement(3856..4413)
                     /locus_tag="PLU43_00715"
     CDS             complement(3856..4413)
                     /locus_tag="PLU43_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO26956.1"
                     /translation="MIYLQLFISFIQVGLFSIGGGYAAIPLIQTQVVEERGWLSMNEF
                     TNLITIAEMTPGPISVNSATFVGIRIAGIGGAVIATFGCILPSCFIVSLLAYLYYKYK
                     GVAALENILSCLRPAVVALIAAAGLSILTLVLFNGAAIKAENVDWIGAGIFIAALFVL
                     KKWKMNPILVMCFCGALGLCLNLLL"
     gene            complement(4410..4997)
                     /locus_tag="PLU43_00720"
     CDS             complement(4410..4997)
                     /locus_tag="PLU43_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO26957.1"
                     /translation="MENEIQSYGKTKTLVTLFLSTLYISAFTFGGGFVIVTFLKKKFV
                     DKLQWIDEKEMLDLTALAQSSPGAIAVNAAMLVGWRVCGITGTVVAIIGTILPPMIIL
                     SIVSGFYTAFSSNLYVALVLKGMQAGVAAVILDVSCSLGKAAVKEQSLINYLIIAAAF
                     VADFFLDVNVVYIILAAALIGVGKEIIQRRRRIKT"
     gene            5104..5991
                     /locus_tag="PLU43_00725"
     CDS             5104..5991
                     /locus_tag="PLU43_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO26958.1"
                     /translation="MTVRHMKIFIQVFQLQNITRASELLHMTQPAVTRAIREIENYYG
                     VCLFERINRRLLATESAKQLYAQAVHVVDAFDAMEKGLRNWDAFGVIRIGASITLGNF
                     LLPELVCQFQKERPNIKIEVMISNGETLEKALLDNKLDIALIEGRVGESELHSEVIGE
                     DCLVLILPPGHSLLTHKKIELEELPEYPMLLREKGSVGRTYLDNVFAVRGITIHPIWE
                     SVSTQALVKAVSFGIGISFLPELLVRKDIMNGVVCTREVADERFLRKRYIVWHKNKYL
                     TSSMLDFISLCETQPLRDD"
BASE COUNT         1814 a         1131 c         1457 g         1691 t
ORIGIN      
        1 ggacttggtt cactgcgaat atccacattt acatttgcga tcatatatct ggtcgtcggc
       61 attattttca gtttcatttt atcaacattg atttcccggc cgatcattgc actgacgaaa
      121 aagcttgata ttatagcaga aggtgatttt tcacaggaag tggaaggaaa tcttataaaa
      181 atcaaagatg aaacaggcat gcttgcaaga gcgatcgaaa aaatgcgtga ttcggtcagc
      241 agcgtgatca aaacggttat gaacgagtcg aaagcagttt ttgaaaatgt atccagtcag
      301 ggtgaaaagg tttcgcatct gcaggcacag atagaagacg tgtcggcaac gacagagcag
      361 ttatctgcag gcatgcagga gacagcggct tcttcggaag aaatgaatac ggtcatgaat
      421 gagatcgagg agtccgtaga atctattgca ttgaggacac aggaaggttc taaaactgta
      481 aatgagattt atgagagagc aaagatattt aaggaaaagg cggtaaaatc aaaagaaatc
      541 gcttataata tttatgagac gtcttccgaa catttgcagg atgcgatcag gcagtcacag
      601 gatgtggaac agatcaatac gctgtcggaa tcggtactgc agattaccgc acagacaaat
      661 cttcttgcat tgaatgcggc aatcgaagca gccagggcag gagaagccgg aaaaggcttt
      721 gcggtcgttg ccgatgagat cggaagactt gccgagagtt ctaaaaattc cgtagcggaa
      781 attcagaagg ttacgaaaac ggttgttgcg tccgtggaaa atctttcgga aaattcgatg
      841 caggttttgg attttattaa ggataaggtt atggatgact atgacagtct tgtagaggtc
      901 agtgagaaat atcacagtga tgcggatact gtagacaaca tcatgaagga tttcagtgca
      961 aagaccgaag aactgacttc ctcgatcgat aagatcatga attcgatcag cggaattaca
     1021 attgcgacaa acgaaggtgc ggaaggaacg accagcattg cgcagaatgt tgttgcggtc
     1081 agcaatgctg caaaggaagt tgtcgattat gcatctgata cgaaaaataa tgcgacgaag
     1141 cttgtggagg cggtcgaggt atttaaggtc taagaacgat ccataaacgg cagcaggttt
     1201 tggaagtaag tgtcctccct gtgattttta taaaaacggg gaggacattt tactgtgaat
     1261 ttacatcata ataaaagaag tatgatataa taaaaacatc ttgtttcgga aaggaacctg
     1321 tcaatgaagc taactaaaga agaaaaatca tggatcatgt atgattgcgg gaattctgcc
     1381 tattcgatgg cagtgacgac agctctgctg cctattgtat tcggaatgtt tcaaaatgtt
     1441 gaaagcagta tggacttagg gtattttaat tccatagcga gtttgatcgt tgcgttatta
     1501 agtccgatat tcggaacgat agccgattat aaggacaaga aaaagaggtt ttttctgttt
     1561 tttgctgtac ttggcatttt tacgactgcc gcgcttgcgc ttgtttcgcc gtccggtgga
     1621 aaatggcaga ttctgattct gttttttatt ttatccgtaa tcggatttaa cggttcctgt
     1681 attttttatg atgcgtttct tccggatgtg acgtcagacg acaggatgga tggtatatct
     1741 tcacgaggtt ttgcatttgg atatattttc agtgtgattc cattcggaat aagccttctt
     1801 attatttttc ttctggggat ggaccaggcg gccggttatc agatcggatt tgtgatcact
     1861 gcgctatggt ggggattatt cacgatcccg atgatcagaa atgtgaaaca gagatattat
     1921 attgagccgg agcctgcgcc ggtaaaaaac agttttatcc gtttgtttcg tactttaaaa
     1981 aatatcatac agtataaata tgtatttatt tttctgctgg cttatttttt ctatattgat
     2041 ggggtggata cgattataaa aatggtagtt ccgtacgcta ccgcgattct tggcggcaat
     2101 gccgtgagta cgtttaccct gcttggaatt cttctgatca ttcagatcgt ggcatttcca
     2161 tgcgcgatcc tgtacggaaa tctggcaaag aagtacagcg cacgcgttat gatcattgcc
     2221 ggtattttta cgtatatcgt tgcctgtatt gcggcattct ttatttcttc cgtatggcat
     2281 atttttatac ttggggtatt gatcggttct gcacagggag ggatccaggc attgagccgt
     2341 tcgtattttg caaagatcat tccgaaggag cggtcaaatg aatttttcgg attttataat
     2401 gtattcggca aattttcagc tattatcgga ccttttctta tggctctgac aacaaatatg
     2461 acgggaaatg cgaggtatag tattttatca attattccgt tgtttattat tggatttttc
     2521 gtatttctaa cgttgccgaa gtcggcagct gccgtaataa aggagtaatt attttgaaga
     2581 aaaacgtgaa tcaaaaaaaa tatctgatcg taatttccta cgacgctttt tcagaagata
     2641 actgggaatt tgcatcaaag ctgcctcatc tttcctcgct gattggcagg ggtgtccaca
     2701 gtacggatct ttgcagtatt tatccgaccc tgacatatgc ggttcatacg acgatggtta
     2761 ccggagagta tcccgatgtg cacggtgtga tccataataa tccgtttcag ccgtttgtgg
     2821 aggaaaatga ccagcgctgg tactggtaca ggaatgaagt gaaggcgccg acagtttatg
     2881 atgtatgcag ggagcatggt cttacgacag cgggtctgct gtggccggtc acggggaagt
     2941 cttccattca atataatata ccggaagtga aggcgatcgg aaaggaaaac cagatcataa
     3001 aaatgcttcg cagcggaagt cctgtattca gtattttaat ggaactaaaa cacggaaaaa
     3061 ccagaaaagg gatcagccag ccctacctgg atgattttac ggcgcaatgc tgtgcggata
     3121 cggtcgtaaa aaaacagccg aatgttatga tgctccattt tacggatctg gacacccaga
     3181 agcatgattt tgggacgaaa agcagacagg tggaagaagc gatgatcaga atggatgccc
     3241 gcatcggtct gatattggat gccataaaaa gatctgggaa tgaagcatct acgacagtta
     3301 tgatattggg cgatcatggc cagatggatg tcagatatca gatccgtctg aatgctgcct
     3361 tttaccggga agggttgatt tataaagaac agggtacgct gaactggcgg gcgtatgtgc
     3421 agggggcgga tggatgtgcc tatttatatg ttcgtgcaca tgatgaagag gctaaggaga
     3481 aagcatttgc aattattcat gaaatgatga aagagaaaaa ttccggaata gaattgcttt
     3541 ttacagggga agatatcagg gaaaagcatc ttgttccttc catggaagct gttctggaag
     3601 cacgtaacgg cttttatttt aatgattcca tagagggacc ggtcatattt gatctggaaa
     3661 aagcaggtct gcgccatgca acacatggat atctgccgca gaagccaggc tacaaatgct
     3721 gttttctgat ggcaggaaac ggaatcaggc aagggcagga actgaaagga attcatatga
     3781 cggatattgc gccgacaatg gcagcggtta tgggagttga atttccttat ggaaaagggc
     3841 ggatattgaa agtttttaca gcagaagatt caaacacaaa cccagagcac cgcaaaagca
     3901 cataactaaa atcggattca ttttccactt tttaagtaca aataaagcgg caataaagat
     3961 gccggctcct atccagtcta cattttcagc cttaatagcc gcaccattga aaagcaccag
     4021 agtcagaata gaaagccctg ccgccgcaat cagagccaca actgcgggtc ttaggcagga
     4081 caggatattt tccagtgctg caacaccctt atatttataa tacaaatatg ccaataagga
     4141 aacgataaaa caggacggca gaatacagcc aaaagttgct atgacagcgc ctccgattcc
     4201 ggctatccgt atgccgacaa aagttgcgga attaacagat atggggcctg gtgtcatttc
     4261 ggcaatcgtg ataagattgg taaactcatt catactgagc cagccgcgtt cttccacaac
     4321 ctgtgtctgt ataagaggaa ttgccgcata tccgccgccg atactaaata atccgacctg
     4381 gataaagctg ataaaaagct gcagataaat catgttttta tcctccttct gcgttgaatg
     4441 atttctttgc cgactccgat cagagcagca gcaaggataa tataaacaac gtttacatca
     4501 agaaaaaaat ctgcaacaaa tgctgccgct attatgagat agtttatcag agactgctct
     4561 ttcacggcag cttttccaag actgcatgac acgtccagaa tgaccgcagc cacacctgcc
     4621 tgcattccct ttaaaacaag tgcaacgtat agattactgg aaaaagcggt atagaaaccg
     4681 gagacgatcg ataaaattat catagggggc agaatcgtgc cgataattgc cacaacggtt
     4741 ccggttattc cgcagacccg ccagccgaca agcattgcag cgttgaccgc aattgctccc
     4801 ggagaggact gcgccagggc tgtcagatca agcatttctt tttcatctat ccattgcagt
     4861 ttatcgacga attttttctt taaaaatgtt acgataacaa agcctccgcc aaaagtgaat
     4921 gcgctgatat agagtgtgct taaaaacaac gtaaccagtg tttttgtttt accataagac
     4981 tgtatttcat tttccatgca aattctcctt ttataataag tttaaagctt gtcacttaat
     5041 atgtaaaatg ctattatgtt ataatattaa taccaatatt gatatagttg gaggacaaat
     5101 gcaatgacag ttcgtcatat gaaaatattt atacaggtat ttcagctgca aaatataaca
     5161 agggcatcgg aactgctgca tatgacccag ccggcagtaa ccagggcaat tcgcgagata
     5221 gaaaattatt atggtgtatg tctttttgag cggatcaaca ggcgtctttt ggcaacggaa
     5281 agtgccaaac agctttatgc acaggcggtt catgttgtgg acgcatttga cgcgatggaa
     5341 aaaggattgc gaaactggga tgctttcgga gtaatacgca ttggcgcgag tattactctt
     5401 ggtaattttc ttcttccgga attagtatgt cagtttcaaa aagagcgccc caatataaaa
     5461 attgaagtta tgatctccaa cggcgagact ttggaaaaag cactgctcga caacaagctg
     5521 gatattgctt taatagaagg gcgggtcggc gagtctgaac tgcatagtga ggtgatcggt
     5581 gaagactgtc tggtactcat tctgcctcct gggcatagtc tgcttacaca taagaagatt
     5641 gaattagaag agctgcctga gtatccaatg cttctaaggg aaaaaggaag tgtcggcaga
     5701 acttatctgg acaatgtttt tgccgtacgc ggtattacga ttcatccaat atgggaaagt
     5761 gtcagcacgc aggctttagt gaaagctgtc agcttcggca tcgggatatc attcctgccg
     5821 gagctattgg tgcgcaagga tattatgaat ggtgttgtat gcacacggga agttgcggat
     5881 gaacggtttt tgagaaaacg ttatatagtt tggcataaga acaaatatct gacatcttct
     5941 atgctggatt ttatctctct atgcgaaacg cagcctcttc gtgatgatta aaataaaaga
     6001 atccaattca gaaaattatg caacgtaaaa aagccggcag ataaagaaat gatctaccgg
     6061 ctttccgttg ataagtttcc tttactctat ttt
//
LOCUS       DAOSYE010000021         5933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_8848_length_5933_cov_4.150902, whole genome shotgun sequence.
ACCESSION   DAOSYE010000021 DAOSYE010000000
VERSION     DAOSYE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8848_length_5933_cov_4.150902"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLU43_00730"
     CDS             complement(<1..286)
                     /locus_tag="PLU43_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO26959.1"
                     /translation="MSDKKIRAAVITSSDSGYQGIREDVSGPEIKKLLEQSGYEITAQ
                     ILLPDEQDCLSEKMKELCDKGTADLIITTGGTGFSERDRMPEATKAIIERE"
     gene            complement(293..1297)
                     /gene="moaA"
                     /locus_tag="PLU43_00735"
     CDS             complement(293..1297)
                     /gene="moaA"
                     /locus_tag="PLU43_00735"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOO26960.1"
                     /translation="MRDALGREINYIRISVTDRCNLRCGYCIPKEGVRLCTHENLLCF
                     DEIERLVRIFAGLGIDRVKLTGGEPFVRRDFPKLAQKIKEVDGIKKLTVTTNGTFFTR
                     ENAEWALECFDGINISLDTPDRGVYEEMTGVDVFDNVYDGLQRLLFLNAGRDPGTLLK
                     LNCVLTRKNKDDITALAGLAKEQKLAVRFIELMPIGQGKIFLENGKGVLQEAEAVKLL
                     EEAYGKLIPCEENSGNGPAVYYRPEGFCGKIGFISALSHKFCETCNRIRLTTTGYLKL
                     CLQYETGIDLKTLLRNGASDEEIRAVVLQTIKEKPAGHAFCSGKIVQEENRGMSEIGG
                     "
     gene            complement(1290..1766)
                     /gene="moaC"
                     /locus_tag="PLU43_00740"
     CDS             complement(1290..1766)
                     /gene="moaC"
                     /locus_tag="PLU43_00740"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOO26961.1"
                     /translation="MENELTHFDKAGNAIMVDVSKKEVTKREAVAEGKIHVSLEVMAA
                     VLGYTSKKGDVLGVARVAGMMAVKQTSALIPMCHILPINTCSIDFEPDEEKLTISAVC
                     TVKTEGKTGVEMEALTGVSVTLLTIYDMCKAIDKRMVLSDIHLVKKSGGKSGEFYA"
     gene            complement(1827..2804)
                     /locus_tag="PLU43_00745"
     CDS             complement(1827..2804)
                     /locus_tag="PLU43_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO26962.1"
                     /translation="MATLKDVAKETGLTVTTVSRVLNNRGYISEETRRNVYAAMKKLN
                     YHPNEVARSLSKQTTNTIGVIVPHIRHPYFADLISNLENEAYLHKYKILLFNSQGKNE
                     KETEYLEMCTSNRVSGVIICSGTVAVEKFKGLNVPLITLERYLENGTAAVECDNFQGG
                     RLAAQHLIDSGCRKLLHLSGVHETSMPADRRAQGFSEVCETNGVFYETIETNVNQYNA
                     LEYHEFIETILKEHPASDGIFASSDLIAAQIIQVCAKLKIRIPEELKIIGFDDVNISS
                     LTTPRITTIHQPIKEMAEAAVELLIKASDQKLVPNRTTLPVTLIKRETT"
     gene            complement(2986..4443)
                     /gene="gtfA"
                     /locus_tag="PLU43_00750"
     CDS             complement(2986..4443)
                     /gene="gtfA"
                     /locus_tag="PLU43_00750"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HOO26963.1"
                     /translation="MKKLENKCMLITYADSMGKDLKDLRLVLNSYFKEAAAGVHILPF
                     FPSSGDRGFAPTDYKKVDPVFGGWSDIEKLAEEYYLMFDYMINHISAQSSYYQDFLKR
                     KDESEYKDLFIRYQEFWKDGEPTEEEVDAIYKRKPKAPYTTACFQDGTSEKVWCTFDE
                     EQIDINCRSAAAKRFIRENLEELCRHGAALIRLDAFAYSTKKAGTSCFFLEPDVWELL
                     EECEEIINPFGAHLLAEIHEHYSMQKKLEEKNYYVYDFALPMLLIHALYYGKTDYLKN
                     WLTICPRKQFTTLDTHDGIGVVDVKDLLPDEEIKKTKEDIFKFGANVKKVYNTAVYNN
                     LDIYQVNCSYYSALGNNDKKYLIARAVQFFAPGIPQVYYVGLLAGKNDLELLEKTKVG
                     RNINRHYYTLQEIEEEVKRPVVKKLLGLMKFRNTHPAFDGGFQMEKCKDTQLHLIRRH
                     KEHKAELKVDFATDKMEIFYTENGIMKRFDPDKEA"
     gene            complement(4456..5322)
                     /locus_tag="PLU43_00755"
     CDS             complement(4456..5322)
                     /locus_tag="PLU43_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO26964.1"
                     /translation="MSETGRKVGGVKRKVLELLGFIGVIILFFLYMFPFLLIILNSFK
                     YKRDIIKNPFSFIVEKGYTFDNFTKAFDKMHFMRAFGNSLFITGLSTLAVIILSAMVA
                     YYFVRAKNGLSKFFFALMAASMIIPFQAIMIPLVSIYGAQLNLLNHRLTLVFLHTGFA
                     MSMSVFIYQGFIKSNIPISLEEAACLDGCNKVQTFFKVVFPLLKPTTSTLVILNVLAF
                     WNDYLLPSLVLGKKQLYTLPLSTYTFYGTYSADYGVIMAALVLTVAPILILYLFLQKQ
                     IINGVVAGAVKS"
     gene            complement(5319..>5933)
                     /locus_tag="PLU43_00760"
     CDS             complement(5319..>5933)
                     /locus_tag="PLU43_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO26965.1"
                     /translation="FVLAYALTNGMKGQNFFRTGFFTPNLIGGVILGFIWQFVFSRVL
                     VNIGDATNWGIFSTSWLSDPTKAFWSLVIVTVWQLSGYMMLIYIAGFTGLSTDVLEAA
                     SIDGATGFSKIKDIILPLMVPSFVICIFLTLSRAFMVYDVNLTLTGGEPYGTTRLVAM
                     HVYEKAFTSRNYGVGQAEALVLFILVACISGAQIYIGKKREVEA"
BASE COUNT         1490 a         1561 c         1126 g         1756 t
ORIGIN      
        1 cttctctttc aataatggct ttggttgcct ccggcatgcg gtctctttcg gaaaaccccg
       61 taccgcctgt tgttatgatc agatccgctg tccccttatc acagagttcc ttcatttttt
      121 cggaaaggca gtcctgttca tccggcagga gtatctgtgc cgtaatttca tatccggact
      181 gttccagtaa ttttttgatt tcaggcccgc ttacatcttc tctgattccc tgatatccgg
      241 aatcactgga tgtaatgacc gcagcccgta tttttttatc gctcatcccg cttcagcctc
      301 cgatctccga catgccccga ttttcttcct gcacgatctt tccgctgcag aatgcatgcc
      361 ctgccggctt ttcttttatt gtttgtaaaa caacagccct gatctcctca tctgacgcgc
      421 cgtttctaag aagtgtcttt agatcgatcc cagtttcata ctgcagacat aatttcaaat
      481 atcctgttgt tgtcagacgg atacgattgc acgtctcgca gaatttatgg gaaagggcac
      541 tgataaaccc gatcttcccg caaaagcctt ccggtctgta ataaacagcc ggaccatttc
      601 cgctgttttc ttcacaggga atcagtttcc cataggcttc ctccagaagc tttactgctt
      661 ccgcttcctg caaaacgccc tttccgtttt ccagaaagat ttttccctgt ccgatcggca
      721 tcagttctat gaaacgcact gccagtttct gttcctttgc gagtcctgcc agcgctgtaa
      781 tatcatcttt atttttacgg gtcaggacgc agttcagctt gagcagtgtc cccggatccc
      841 gtccggcatt cagaaaaagc agcctctgca gaccgtcata aacgttgtca aacacatcaa
      901 cgcctgtcat ttcttcataa acaccgcggt ctggcgtgtc aagactgata ttgatcccgt
      961 caaagcattc cagtgcccat tctgcattct cccgggtgaa aaaagttccg tttgtcgtaa
     1021 ccgtcagttt tttgatcccg tcgacttctt tgattttctg tgccagcttg ggaaaatccc
     1081 ttcttacgaa cggctccccg cccgtcagct ttaccctgtc gatgccaaga cctgcaaaaa
     1141 tccgtacaag cctttctatt tcatcaaaac agagaagatt ttcatgtgta caaagccgta
     1201 caccttcttt cggaatacag tatccgcacc ttaaattaca tctgtccgtt actgaaattc
     1261 tgatataatt gatttctctg cccaatgcat cacgcataaa attcgccgct ctttccgccg
     1321 ctctttttta caaggtgaat atcagagagc accatccgct tatcgatcgc cttgcacata
     1381 tcatagatcg taagcagcgt gaccgacacg cccgtcagcg cttccatctc caccccggtc
     1441 ttcccttcgg tttttaccgt acaaaccgca gagatcgtca gcttttcttc atccggctca
     1501 aaatcgatgc tgcatgtatt gatcggcaat atatgacaca tcggaatcaa tgcacttgtc
     1561 tgcttgactg ccatcatacc ggcgactctc gcaacaccga gtacatctcc ttttttcgaa
     1621 gtataaccaa gaactgctgc catgacctcc aaacttacat ggatctttcc ttctgccaca
     1681 gcttctctct tcgtaacttc tttttttgaa acatctacca taatcgcatt tcccgcctta
     1741 tcaaaatgcg tcagttcatt ttccataatt gttcccttcc attctcgccc cgctgctgcc
     1801 attttatcat actctatcgg tttttgctat gtcgtttccc gttttatcag cgtaacagga
     1861 agcgttgttc tgtttggaac cagtttctga tcacttgcct tgatcagcag ttccaccgcc
     1921 gcttccgcca tttccttgat cggctgatgg atcgtcgtga tccggggcgt tgtcagagaa
     1981 gaaatattga catcatcaaa acctataatt tttaattcct ccggaatacg gatctttaat
     2041 tttgcacaca cctgtatgat ctgcgccgca atcagatcac tgctcgcaaa aatgccatcc
     2101 gaagccggat gctctttcaa aatggtttca atgaattcat ggtattccaa agcattgtac
     2161 tgatttacat ttgtttcgat cgtctcataa aaaactccgt ttgtttcgca tacctcactg
     2221 aatccctgcg cgcgccggtc cgccggcata gacgtttcat ggacaccgct taaatgaagc
     2281 agcttcctgc agccgctgtc tatcaaatgc tgtgcagcca gccggcctcc ctgaaaattg
     2341 tcacactcca ccgcagccgt tccattctcc agatacctct ccagcgtgat cagcggtacg
     2401 ttcaggccct tgaatttctc cacggccacc gttccgctgc agatgataac gcccgatacc
     2461 cgattgctcg tgcacatttc cagatattct gtttcttttt cattttttcc ctgggaatta
     2521 aacagtaaaa tcttgtactt atgcagatat gcttcatttt caagattact gatcaaatcg
     2581 gcaaaatacg gatggcggat atgcggcacg atcactccga tcgtgttcgt cgtctgtttg
     2641 gaaagggagc gcgccacttc attcggatga taattcagct tcttcattgc cgcataaacg
     2701 tttctgcgtg tttcttcgct gatatacccg cgattattca atactctgga aaccgtagta
     2761 accgtcagac ccgtttcctt tgccacatcc tttaaagtcg ccatttcttc tctcctgttg
     2821 ccgcattgtt atttgccgtt cacagcgttt ccgccaaaat tgatcaaaag ctgtataaat
     2881 tataccacga ttcacaccgc aattgccata taaaaacaga agaagggacg ttccaaggcc
     2941 tctgcttttt caaagccttt taaacgtccc tccaaaatta tgcgcttacg cttccttatc
     3001 cggatcaaat ctcttcatga tgccgttttc agtataaaaa atttccattt tgtctgttgc
     3061 aaagtccact ttcagctctg ctttatgctc tttatgtctc cgtatcagat gcagctgtgt
     3121 atctttgcat ttttccatct gaaagccgcc gtcaaatgca ggatgcgtat ttctgaattt
     3181 catcagacca agcagttttt ttacgaccgg ccgtttcact tcttcttcga tttcctgcag
     3241 tgtataataa tgtctgttga tattacggcc gacctttgtc ttttctaaaa gctccagatc
     3301 gttttttccg gcaagcagcc ccacataata cacctgcggg atacccggtg caaaaaactg
     3361 tacggcacgt gcgatcagat attttttatc attatttccg agcgcggaat aataagaaca
     3421 attcacctga tagatgtcca gattattata gacggccgta ttataaactt ttttgacatt
     3481 tgcgccgaat ttaaaaatat cctcttttgt tttctttatc tcttcatccg gaagcagatc
     3541 ttttacatcc acgaccccga tcccgtcatg agtatccagc gtcgtaaatt gttttctcgg
     3601 gcagatggtc agccagtttt tcaaataatc ggtctttccg taatacagtg catggatcag
     3661 aagcatcgga agtgcaaaat catatacata gtaatttttt tcttccagct ttttctgcat
     3721 ggaataatgc tcatggatct ccgcaagcag atgcgcacca aagggattga tgatctcctc
     3781 gcattcctct aaaagctccc agacatccgg ttcgagaaag aaacagcttg tacccgcctt
     3841 tttcgtggag tacgcgaatg cgtccaggcg gatcagtgct gctccatgcc tgcagagctc
     3901 ttcaaggttt tctctgatga accttttggc cgcagcagat ctgcagttga tatcgatctg
     3961 ttcctcatca aaggtacacc agaccttttc agatgttccg tcctgaaagc aggctgtcgt
     4021 atagggtgcc ttcggctttc ttttatagat cgcgtcaacc tcttcctctg tcggttctcc
     4081 gtctttccag aactcctgat agcggataaa cagatccttg tattccgact cgtccttcct
     4141 tttcaaaaaa tcctggtaat agctgctctg tgcggaaata tgattgatca tataatcaaa
     4201 catcagataa tactcttccg ccagtttttc aatatcactc cagccgccaa ataccggatc
     4261 tacttttttg tagtccgtcg gcgcaaaccc tctgtctccg gaagacggaa aaaaaggtaa
     4321 gatatgcacg cccgcagctg cttctttaaa atagctgttt aaaaccagcc gcagatcctt
     4381 taagtctttt cccattgaat ccgcataggt aatcagcata catttgtttt ccagtttttt
     4441 catctctgtt cctctttatg attttaccgc acctgcgacc acaccgttga tgatctgttt
     4501 ctgcaggaat aaatacagga tcagtatcgg tgcaacagtc agtaccagtg ctgccataat
     4561 tactccgtaa tccgccgaat aggttccata aaatgtatag gtggacagcg gaagcgtata
     4621 cagctgcttt ttgccgagca ctaacgacgg cagcagatag tcgttccaaa aggccagtac
     4681 gttcaatatc acgagcgttg atgtcgtcgg cttcagaagc ggaaacacaa ccttgaaaaa
     4741 tgtctgtacc ttgttgcatc cgtccaggca ggctgcctcc tccagtgaga tcggaatatt
     4801 gcttttgata aatccctgat agataaatac cgacatactc atggcgaatc ccgtatgcag
     4861 aaatacgagc gtcagccgat gattcagcag attcagctgt gcgccgtaaa tggatacgag
     4921 cggaatcatg atcgcctgaa acggaatgat catcgaagca gccatgagtg caaagaaaaa
     4981 tttcgaaagg ccgtttttgg ccctgacaaa ataatatgcc accattgcag atagtatgat
     5041 gactgccagc gtactaagac cggtaataaa taatgaattt ccaaacgctc gcataaaatg
     5101 catcttatca aatgcttttg taaaattatc aaaggtatat cctttttcca caataaagga
     5161 aaatggattt ttgataatat ctcttttata tttaaatgaa ttcaatatga tcagaaggaa
     5221 cggaaacata tacagaaaaa acagaattat gactccgata aaccctaaga gctccagtac
     5281 ttttcttttc acaccgccta cttttctgcc cgtctcactc atgcttccac ctcccgcttc
     5341 ttaccgatat agatctgcgc tccgctgatg catgccacaa gaatgaaaag gacaagtgct
     5401 tccgcctgac cgactccgta atttctggag gtgaatgcct tttcatacac gtgcattgcc
     5461 acgagccttg tcgttccgta aggctccccg ccggtcagtg ttaagtttac atcatacacc
     5521 atgaaggccc gtgaaagtgt caaaaaaata cagatcacaa aagacggaac catcagcggc
     5581 aggataatat cctttatttt ggaaaatccg gtcgcgccgt cgatgctcgc agcctcaagt
     5641 acatccgtgg acaatccggt aaatcccgca atatagatca gcatcatata accggacagc
     5701 tgccatacgg tcacgatgac aagggaccag aacgcttttg tcggatcgga cagccatgag
     5761 gtggaaaaga tcccccagtt tgtcgcatcc ccaatattga caagtactct ggaaaatacg
     5821 aactgccaga taaaaccgag gataacgcct ccgatcaggt ttggcgtaaa aaagcccgtt
     5881 ctgaaaaaat tctgtccttt cattccgttc gtcagcgcat acgcgagaac aaa
//
LOCUS       DAOSYE010000022         5783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9202_length_5783_cov_3.774267, whole genome shotgun sequence.
ACCESSION   DAOSYE010000022 DAOSYE010000000
VERSION     DAOSYE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_5783_cov_3.774267"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLU43_00765"
     CDS             complement(<1..554)
                     /locus_tag="PLU43_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO26966.1"
                     /translation="MSDYILEMNHITKDFSGVKALDDVNLQVKRGEIHALCGENGAGK
                     STLMNVLSGVYPHGTYIGDIVYKGEACKFHNLKDSEAKGIVIIHQELALSPLLSIAEN
                     VFLGNEQTSSRGIIDWTKTRKHAQEVLSKVGLEHEDVNAPVNSLGVGKQQLIEIAKAM
                     AKKVELLILDEPTAALNDEESRKLL"
     gene            complement(666..1856)
                     /gene="chvE"
                     /locus_tag="PLU43_00770"
     CDS             complement(666..1856)
                     /gene="chvE"
                     /locus_tag="PLU43_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO26967.1"
                     /translation="MKKKVLGALLSVAMVASLLAGCGSTATEEAAPAAAEETEATEEA
                     APAAAEETEAAEEETAAADTTGMKVAVAMPTQSSERWINDGANMKEKLEALGYEVDLQ
                     YAEDDVQAQVSQIENMIASGANCLVIAAVDSSALVNVEAQAKEAGIPIIAYDRLLMDT
                     DAVSYYATFDNKGVGTAIGKYIEEAKDLPNAEANGESYTVEFFMGSPDDNNAVMLYNG
                     LMEVLQPYLDSGVLVCESGRTSFDDTCILRWSQETAQQNCENYLTGFYADKKLDICAT
                     AFDGFAYGCKAALEGAGYTVGDDWPLITGQDAELMATKNIISGAQTMSIYKDTRLLAE
                     KCVTMVQAVLEGSEPEINDTEQYDNGKIVVPSYLCTPVAVDKSNYEEIIVDGGYYTEE
                     QLAE"
     gene            complement(2069..3067)
                     /locus_tag="PLU43_00775"
     CDS             complement(2069..3067)
                     /locus_tag="PLU43_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO26968.1"
                     /translation="METVYTANEKRYEKMVYNRVGKSGLKFPAVSLGFWHNFGSRDNF
                     DTMRAMCRTAFDAGITQFDLANNYGPIYGSAEENAGRILSTDFKPYRDELVITTKAGY
                     DMWPGPYGNWGSKKYLTASLDQSLKRLQQEYVDIFYHHRMDPETPLEETMEALAAAVR
                     SGKALYAGISNYDREQTAKAAGILKELHCPCIVNQRSYSIFNRDIEKDGVKTYCKEAG
                     IGIIAFSPLSQGLLTDKYLHGIPADSRIAKDGRFLKQSALTPERLKQIEALNELAVMR
                     GQTLAQMALSWILKDQDVCSVLIGASRPEQITENIGIVSQTQFTLEEIRKIENICI"
     gene            complement(3102..3623)
                     /locus_tag="PLU43_00780"
     CDS             complement(3102..3623)
                     /locus_tag="PLU43_00780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOO26969.1"
                     /translation="MKRVLIVVDMQNDFIVGSLGTREAQGIVENVKCKIKQYEDRGDT
                     VIFTRDTHTNEYLFTLEGKNLPVEHCIRGTAGWMIADGLEGAQSYIYVDKPSFGWLGW
                     KKLFEENKLDPSEIELCGLCTDICVVSNALILKAVFPETPLSVDASCCAGVTPKTHSA
                     ALETMRMCQVTIL"
     gene            complement(3655..5163)
                     /locus_tag="PLU43_00785"
     CDS             complement(3655..5163)
                     /locus_tag="PLU43_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOO26970.1"
                     /translation="MIDQSIAALAEYAVQQGLITEEEKTYSVNLLLDILRLDEFKEPE
                     DRKKTVQSAKNLEQILKDICDYAYENDLIEENSVVYRDLFDTRVMNCFVPRPSEVIRI
                     FYEKYAHSPKEATDYYYHLSQASDYIRTYRIAKDIKWTTDSKYGPIDITINLSKPEKD
                     PKAIAAAKNAKQSGYPKCLLCVENEGYAGRINHPARENHRIIPITICGQKWGFQYSPY
                     VYYNEHCIVLNTQHVPMTINREVFEKLFDFLRQFPHYMICSNADLPIVGGSILSHEHF
                     QGGNFEFPMARAMVEKRIVVPGFEEIEAGILHWPLSAIRIRSKNQKRLTALADHILTA
                     WRNYTDEDAFIFAKTDGVPHNTITPVARKAGGLYELDLTLRNNITTEDRPLGVYHPRG
                     EYHHIKKENIGGIEVMGLAILPSRLKSELEGLAAEILAGREISADETLKKHAAWVKNF
                     LPRYEGVINAENIMDILKEETGKVFVKVLEDAGVFKCTKEGRVHFMKFINSL"
     gene            complement(5205..>5783)
                     /locus_tag="PLU43_00790"
     CDS             complement(5205..>5783)
                     /locus_tag="PLU43_00790"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO26971.1"
                     /translation="TNTYGETKLSMEKMFKWVGKAYGLRYVSLRYFNACGAHESGVIG
                     EAHNPESHLIPLILQVPNGQREHISIFGEDYATKDGTCVRDYIHVTDLAQAHMLAVKY
                     LMDGKESDLFNLGNGIGFTVKEVIEAARKVTGYPIQAAVEQRRAGDPAFLIASSEKAK
                     KILGWKPQFADIETIIASAWKWHAAHPVGYEK"
BASE COUNT         1384 a         1534 c         1030 g         1835 t
ORIGIN      
        1 agaagcttcc ggctttcctc atcgttcaag gctgccgtag gctcatccag gatcaaaagc
       61 tccacctttt ttgccattgc tttcgcaatt tcaatcaact gctgttttcc gacaccaagt
      121 gagttaacag gagcattgac atcttcatgt tccagaccca ccttgctaag tacttcctgc
      181 gcatgtttcc ttgtcttcgt ccaatcaata attcctctgg aagaagtctg ttcatttcct
      241 aaaaacacgt tttcagcaat ggacagcagc gggctcagcg ccagctcctg atgaataata
      301 acaatgccct ttgcctcgct gtctttcagg ttatgaaatt tgcatgcttc ccctttatac
      361 acaatatctc ctatgtacgt accgtgagga tatactccgg acaaaacatt catcagagtg
      421 gattttcctg ccccgttttc accacacaac gcatgaattt ccccgcgttt aacctgaaga
      481 tttacatcat ccagtgcttt cacgcctgaa aagtcttttg taatatggtt catttccaag
      541 atgtaatccg acatcttctc agctcctttt ctcatattta aaattttgta tgaaatacgg
      601 gcggcagcaa ttctgccgcc acccatactt tatctttctt ttatagcgtc ttcttaacac
      661 tctgcttact cagccagctg ttcttccgta taatatccgc cgtctacaat aatttcttcg
      721 taattgctct tgtcaacagc taccggtgta cacagataag aaggaactac gatcttacca
      781 ttgtcatact gctccgtatc gttgatctcc ggctccgaac cttccagaac tgcctgtacc
      841 atcgttacgc acttctcagc aagaagacgt gtgtctttgt aaatagacat tgtctgagcg
      901 ccgctgataa tattcttcgt tgccataagc tctgcatcct gacctgtaat aagcggccag
      961 tcatctccta ccgtatagcc ggcaccttca agagctgctt tacatccata agcaaaacca
     1021 tcaaaagctg ttgcgcagat atcaagcttc ttatccgcat agaaacctgt cagataattc
     1081 tcacagttct gctgtgctgt ttcctgtgac catctcaaaa tacaggtatc atcaaaagat
     1141 gttctgccgg attcgcaaac aagcacgccg ctgtcaagat acggctgaag aacttccatt
     1201 aaaccgttgt ataacattac ggcattatta tcatccggtg aacccatgaa gaactcaact
     1261 gtataagatt ctccgtttgc ttctgcattc ggcagatcct ttgcttcttc gatatacttg
     1321 ccgattgccg taccaacgcc tttgttatca aatgttgcat aataggaaac ggcatccgta
     1381 tccattaaca gacgatcata tgcaatgatc gggattcctg cttcttttgc ctgtgcctct
     1441 acatttacaa gtgcggaaga gtcaaccgct gcgataacaa gacagtttgc accgcttgcg
     1501 atcatatttt caatctgaga aacctgagcc tgtacatcat cctctgcgta ctgaagatct
     1561 acttcataac caagtgcttc caatttttct ttcatgttgg caccgtcatt gatccatctt
     1621 tctgatgact gcgtaggcat tgcaacagca accttcatac ctgtcgtatc tgctgcagct
     1681 gtttcttctt ctgctgcttc tgtttcttct gctgctgcag gagccgcttc ttctgtcgct
     1741 tctgtttctt ctgctgctgc aggagctgct tcttctgttg ccgtgctgcc gcaccctgcc
     1801 agaagagatg ctaccattgc tacgctgagt aatgcaccta aaactttctt cttcatacta
     1861 aataatcctc cttatttttt ggcggctgct aaatttagca cccatattcc ttaactacat
     1921 gctcagtata acaatgtgac tttttaaaat acatataaaa tcctactgtc taccatgaaa
     1981 attctgcaca tattctccaa aataaaaaat gatgcaaaat atttctattt ttttgcatca
     2041 ttttcttttt tatgattgat ctatgtaatt atatacaaat attctctatt ttccggatct
     2101 cttccaaagt aaattgtgtc tgcgatacga tcccgatatt ttctgtgatc tgttccggtc
     2161 tggatgcccc gatcagtacg gaacagacgt cctgatcctt cagtatccat gacaatgcca
     2221 tctgcgccag tgtctgccct ctcattactg cgagttcatt taaggcttct atctgtttta
     2281 agcgttccgg cgtcagtgcg gactgcttta aaaatcttcc gtcttttgca atcctgctgt
     2341 ctgccgggat cccgtgcaga tatttatccg tcaaaagtcc ctgggacagc ggactgaatg
     2401 caatgatccc tattcccgct tctttacagt aggtttttac cccatccttc tcaatgtccc
     2461 tgttaaatat ggaatagctt cgctgattta caatacacgg gcagtgcagt tcctttaaga
     2521 tcccggcggc ctttgccgtc tgttctctgt cataattgga aatacccgca tacaaggctt
     2581 ttccgcttcg gacagcggct gccagcgcct ccattgtttc ttccagcggc gtttccggat
     2641 ccattctgtg atgataaaaa atatccacat attcctgctg cagtcttttt aaactctgat
     2701 caaggctggc cgtcagatat tttttgcttc cccagttgcc gtaaggaccc ggccacatgt
     2761 catagcctgc ttttgtagtg atcacaagtt catccctgta tggtttgaaa tcagtactca
     2821 atatcctgcc tgcattttcc tcggcacttc catatattgg tccatagtta ttcgcaaggt
     2881 caaactgcgt gatccctgca tcaaatgctg tccggcacat tgctctcatc gtatcaaaat
     2941 tatccctgct gccgaaatta tgccaaaatc caagcgaaac tgcaggaaat ttcaatccgc
     3001 tttttccgac acgattatag accatctttt catatctttt ttcatttgcc gtataaactg
     3061 tttccatgta tcttctccct tcaattctgc ttcgaatttt gttataaaat cgtgacctgg
     3121 cacatccgca tcgtctccag tgccgcacta tgtgtctttg gcgtcacgcc tgcacagcag
     3181 gaggcatcta ccgacagcgg cgtttccgga aataccgctt ttaaaatcaa tgcattggat
     3241 accacgcaga tatccgtaca tagaccgcac aactcaattt ccgacgggtc cagcttattc
     3301 tcttcaaaaa gctttttcca tcccagccac ccaaaggatg gtttatccac atagatatat
     3361 gactgcgccc cttccagtcc atctgcgatc atccagccgg ctgttcccct gatgcaatgc
     3421 tctaccggca gatttttgcc ttccagcgtg aacagatatt cattcgtatg cgtatctctt
     3481 gtaaagatta cagtatcacc gcggtcttca tactgtttta ttttgcattt tacattttca
     3541 acgattccct gcgcctctct cgttcccagg gaaccgacaa taaaatcgtt ctgcatgtct
     3601 acaacgatca gcactctttt cattttccac cggtcctctc ttttcatttt ccggtcaaag
     3661 actgtttatg aatttcataa aatgtactct tccctctttt gtacatttaa agacacctgc
     3721 atcttccagt actttgacaa atacctttcc cgtttcttcc ttcagaatat ccataatatt
     3781 ttccgcattt ataacgccct cataacgcgg taaaaaattt tttacccagg cggcatgctt
     3841 tttcagcgtt tcatctgcgc tgatctccct gcctgcaaga atttccgcag caagtccttc
     3901 caattcagat ttcagacggg acggcaggat agcaagtccc atcacttcga tccctccaat
     3961 gttttctttc ttaatatgat gatattctcc gcgcggatga taaacgccga gcggccggtc
     4021 ttctgttgta atattgttgc gcagcgtcag atccagttca taaagtcccc ctgcttttct
     4081 ggctacgggt gtaatcgtat tatgcggcac accgtccgtc ttcgcaaata taaatgcgtc
     4141 ttcgtccgta tagtttcgcc atgctgttaa tatatgatcc gccagagccg taaggcgttt
     4201 ctgatttttg ctgcggatac ggatcgcgga cagcggccag tgaagaatcc ccgcttcgat
     4261 ctcctcaaaa ccaggcacaa cgattctttt ttccaccatg gctcttgcca tcggaaattc
     4321 aaaattccct ccctgaaaat gctcgtggct gagaatggac cctcccacga tcggcagatc
     4381 cgcattggag caaatcatat aatgcggaaa ctgacgcaga aaatcaaaca gtttttcaaa
     4441 gacctctctg tttatcgtca ttggtacatg ctgtgtgttc aaaacaatgc agtgctcatt
     4501 ataatataca tagggagaat actgaaaacc ccatttctga ccgcagatcg taatcggaat
     4561 gatcctgtga ttttctcttg ccggatgatt gatcctgcct gcatagcctt cattttcaac
     4621 acagagcaga catttcggat atcctgactg cttcgcgttt tttgcagctg cgatcgcttt
     4681 cggatctttt tccggcttag acaggttaat ggtaatatcg atcggtccgt acttactgtc
     4741 ggtcgtccat ttgatatctt ttgcaattcg atacgttctg atataatccg acgcctgtga
     4801 caggtgataa taataatcgg ttgcttcctt cggagaatgt gcatattttt cataaaatat
     4861 ccggatcact tcagacggcc gcggtacaaa acaattcatg actcttgtgt caaataaatc
     4921 ccggtaaaca acgctgtttt cctcgatcag atcattttca tacgcataat cacagatatc
     4981 tttcaggatc tgttcaagat ttttcgcact ctgcactgtc tttttccgat cctctggttc
     5041 cttaaattca tccagcctta atatgtccag caaaagattc acggaatacg ttttttcttc
     5101 ttccgtaatc agtccctgct gcacggcata ttccgctaac gcggcaatac tttgatcaat
     5161 catgcttctc ctctctttcc aaaacagacc catatgttcc ttttctattt ctcataacca
     5221 acaggatgtg cggcatgcca tttccatgca gaggcaatga tcgtctcgat atcggcaaac
     5281 tgcggcttcc agccaagtat ctttttcgct ttttcacttg acgcgatcaa aaatgccgga
     5341 tctcccgcac gtctttgttc cacagcagct tggatcggat atcccgttac ttttcttgcc
     5401 gcctcgatca cttcctttac cgtaaatccg attccgtttc caagattaaa cagatcgctc
     5461 tcctttccgt ccattaagta ttttacggca agcatatgtg cctgtgccag atccgtcaca
     5521 tgaatatagt ctctgacaca tgttccgtcc tttgtcgcat agtcttcgcc aaaaatactg
     5581 atatgctccc gctgtccgtt cggcacttgt agaatcagcg gaatcagatg actttccggg
     5641 ttatgtgcct ctccgatcac tccgctttca tgcgccccgc atgcgttaaa atagcgaagg
     5701 gaaacatatc gcagcccgta tgcctttcct acccatttga acattttttc catcgaaagc
     5761 tttgtttccc cataagtgtt cgt
//
LOCUS       DAOSYE010000023         5772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9236_length_5772_cov_3.529299, whole genome shotgun sequence.
ACCESSION   DAOSYE010000023 DAOSYE010000000
VERSION     DAOSYE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_5772_cov_3.529299"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLU43_00795"
     CDS             <1..474
                     /locus_tag="PLU43_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOO26972.1"
                     /translation="LWKISTILQKFSRFMPDTYEGIMTIRDAIKTPAQEEVLEKIFPT
                     FPSISIDYGIMEKSENIYTVPGTFGWDDVGSWLAVERIKRSNECGNIVNGNIVTINTK
                     NCIIQGDKKLIAAVGLEDLIIVDTEDATLICEKDSTADIKKVLENLKICNRSEYL"
     gene            complement(544..1683)
                     /locus_tag="PLU43_00800"
     CDS             complement(544..1683)
                     /locus_tag="PLU43_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOO26973.1"
                     /translation="MKIFWKTRGKLIVLAMTCMMVMALTGCGNKEAEESTQDPETQTD
                     QTAADSTEEEVSDDTADDPNALEEGQMRSYLSGLPVDEKIGNRRPVAIMLNNLEAAQP
                     MSGISYADVVYEYVVEGAITRLLGVFENYDSLDKIGSIRSCREYFVYTALEFDAIYMH
                     FGQAAYAVDLLDQDYVDNLNGLGEAGDTSFYRTTDRQAPHNVYTSAAGIEAGIKKLGY
                     REDHYDGYKGKFKFCDLDKEVTNEDGLSATHIEPNYKINKPWFDYNEETGKYDRYQYG
                     GPQIDDLTGEQISYDNVILQYNNWVQLDAKDYLAFDCHSGGVIQYCTKGKMIAGIWQR
                     EINEDNYDMSAIRYYDLDGNEIEINNGKTFICVVQDTEIENVVIE"
     gene            complement(1885..3060)
                     /gene="dnaJ"
                     /locus_tag="PLU43_00805"
     CDS             complement(1885..3060)
                     /gene="dnaJ"
                     /locus_tag="PLU43_00805"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO26974.1"
                     /translation="MAESKRDYYEVLGVDRNADEATVKKAYRTLAKKYHPDMNPGNAE
                     AEKKFKEASEAYAVLSDPEKKRQYDQFGHAAFENGGAGGFGGFDFSGADFGDIFGDIF
                     GDLFGGGRSRTNHASNGPMKGANLRTSVRITFEEAVFGVDKEIELNLKDVCGTCHGTG
                     AKPGTSPETCPKCGGKGQVVYTTQSLFGVVRNVQTCPDCQGTGKIVKEKCPNCHGTGY
                     ISNRKKIQVSIPAGIDNGQSVRIRDKGEPGVNGGPRGDLLVEVVVARHPIFQRQDTNI
                     FSTAPISYAQAALGGEVLIDTVDGKVIYEVKPGTQTDTKIRLKGKGVPSLRNRQVRGD
                     HYVTLVVQVPEHLQAEAKDLLRRFDEITVNSLHATDGYEEKKSDDAGEKRKKEFWNK"
     gene            complement(3138..5030)
                     /gene="dnaK"
                     /locus_tag="PLU43_00810"
     CDS             complement(3138..5030)
                     /gene="dnaK"
                     /locus_tag="PLU43_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOO26975.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGKPTVIANTEGARTTPSVVAFTK
                     TGERLVGEPAKRQAVTNAEKTISSIKREMGTDHKKTIDSKKYSPQEISAMILQKLKAD
                     AESYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGLDNE
                     KEQKIMVYDLGGGTFDVSIIEIGEGVIEVLATSGNNRLGGDDFDQRITNYMLSEFKKN
                     EGVDLSNDKMALQRLKEAAEKAKKELSSATTTNINLPFITATSEGPKHFDMNLTRAKF
                     DELTHDLVESTTGPVQSALKDAGLTSSELGQVLLVGGSTRIPAVQDKVKQLTGKEPSK
                     SLNPDECVALGASIQGGKLAGDSGAGEILLLDVTPLSLSIETMGGVATRLIERNTTIP
                     TKKSQIFSTAADNQTAVDINVVQGERQFAKDNKSLGQFRLDGIPPARRGVPQIEVTFD
                     IDANGIVNVSAKDLGTGKEQHITITAGSNMSDSDIDKAVREAAEFEAQDKKRKEAIDA
                     KNDADSFIFQTEKAMEEVGDKLDANDKTAVEAELNSLKELVAKADPENMTEEQVVELK
                     AAQEKAMQTAQKLFEKMYEQTQASGAGPDMSNMGGQAGPDMSQGSGGDNAGYGDDVVD
                     GDYKEV"
     gene            complement(5093..5770)
                     /gene="grpE"
                     /locus_tag="PLU43_00815"
     CDS             complement(5093..5770)
                     /gene="grpE"
                     /locus_tag="PLU43_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO26976.1"
                     /translation="MEKDLTEDILKDMTEEHAEESIRTEGEEAEAVQTEAGQSEAEEE
                     PADEKSEESETGEETGEEKAEENKKYFGRKDKKEKKDKKDEMIEDLTDKVKRQMAEFD
                     NFRKRTEKEKSLMYEMGVKSVVEKLLPVIDNFERGLQTVPEEEKDSSFVEGMNMIYKQ
                     LSGGLEQLGVKQIEAVGKEFDPNFHNAVMQVESEEYESGTVAQELQKGYTYRDMVVRH
                     SMVGVVG"
BASE COUNT         1336 a         1576 c         1028 g         1832 t
ORIGIN      
        1 ttatggaaaa tctctacgat cctgcaaaaa ttcagccgtt ttatgccgga tacttatgaa
       61 ggcattatga cgatcaggga cgcaatcaaa actccggcac aggaagaagt tctggaaaag
      121 atcttcccga ccttcccgtc tatttcgatc gattacggca ttatggaaaa atccgaaaac
      181 atttatacgg tccccggaac cttcggctgg gatgatgtcg gatcatggct tgccgtggaa
      241 cggatcaaac gatccaacga atgcggaaac atcgttaacg gcaacatcgt gacgatcaat
      301 acgaagaact gtatcattca gggtgacaag aaactgatcg ccgcggtcgg tttggaggac
      361 ctgatcatcg ttgacacaga agatgcgact ctgatctgtg aaaaagacag cactgccgac
      421 attaaaaaag tactggaaaa tttaaagata tgcaatcgca gcgaatattt gtaacataaa
      481 agacatggat tccacaagcg ctctccggca tttatggaat ccatgtcttt ttacaatcct
      541 gctttattcg atcactacat tttcgatttc cgtatcctgt actacacaaa taaacgtctt
      601 gccgttattg atctcgattt cgtttccgtc cagatcataa taacggatcg cactcatatc
      661 ataattatct tcattgatct ctctctgcca gataccggca atcattttac cttttgtaca
      721 atactggatc acgccgccgg aatgacagtc aaaagcaaga taatcttttg catcgagctg
      781 cacccagtta ttatactgca ggatcacgtt atcataggaa atctgttctc ctgtcaggtc
      841 atcgatctgc ggtcctccat attggtacct gtcatattta cctgtttcct cattataatc
      901 aaaccatggc ttattgatct tgtaattcgg ctcgatatga gtagccgaaa gcccgtcctc
      961 atttgtcacc tctttatcca gatcgcagaa tttaaatttt cctttatagc cgtcgtaatg
     1021 gtcctctctg taaccaagct ttttaatacc ggcttcaata ccggccgcac ttgtatatac
     1081 gttatgcggc gcctgtctat ccgttgtacg gtaaaacgac gtatcaccgg cttctccaag
     1141 cccattcaga ttgtccacat aatcctgatc cagcagatcc accgcatagg ccgcctgtcc
     1201 gaaatgcata tagatcgcat caaattccag cgccgtatag acaaaatact ctctgcagct
     1261 cctgatcgaa cctattttgt ccaagctgtc ataattttca aaaacaccga gcagtcttgt
     1321 aatagcgccc tcaacaacat attcatagac aacgtccgca taagaaattc cgctcatcgg
     1381 ctgtgctgcc tccagattgt tcagcatgat cgccaccggt cttctgttgc cgatcttctc
     1441 atctaccgga aggccgctga gatagctgcg catctgtcct tcttcaagtg cattcggatc
     1501 atctgccgta tcatccgaaa cttcttcctc tgtactgtcc gcagcagtct gatccgtctg
     1561 agtttccgga tcctgagtac tttcttctgc ctctttatta ccgcatcctg tcaaagccat
     1621 gaccatcatg catgtcatag ctaatacgat caattttcct cttgttttcc aaaaaatctt
     1681 cattttaact gcctctcttt attttccttc ccccaataag gcagaaggac tttcagcaaa
     1741 ataatgtagt aatatggatg tatcattcat catcacaaat cataaaataa tacataaaat
     1801 acgggctgca tgtattcctg ataaatgcac gcaacccata ttataattta tttgaccgta
     1861 taaatcaatc ttttcttttt aatcctactt gttccaaaat tccttttttc ttttttctcc
     1921 ggcgtcatcc gatttctttt cctcatagcc gtcagtcgca tgaagtgaat ttaccgtaat
     1981 ctcatcaaat ctgcgcagca gatcttttgc ctccgcctgc agatgctccg gcacctgtac
     2041 aacaagcgtt acatagtgat ccccgcgaac ctgtctgttt cgaagcgacg gcacgccctt
     2101 gcctttcagc ctgatttttg tatctgtctg tgtacccggt tttacttcat aaataacctt
     2161 gccgtccacc gtatcgatca gtacctctcc gccaagtgct gcctgcgcat aagagatcgg
     2221 tgcggtagag aaaatatttg tatcctgcct ttggaatatc ggatgtcttg caacaaccac
     2281 ttccacaagc agatctcctc tcggtccgcc gttcacgcca ggttcgccct tgtccctgat
     2341 ccgtacgctc tgtccgttat caatacctgc cggtatggat acctgtatct tttttctgtt
     2401 tgaaatatag ccggttccat gacagttcgg acatttttcc tttactatct ttcctgtacc
     2461 ctggcagtcc ggacaggtct gtacatttct gaccacaccg aacagcgact gtgtcgtata
     2521 gacgacctga ccttttccgc cgcattttgg acacgtttcc ggactggtgc ccggctttgc
     2581 tcccgtgcca tgacacgtac cgcacacatc cttcagattc agttctattt ccttatccac
     2641 tccaaatacg gcctcttcaa acgtgatcct gacgctcgtt cgcagattag cgcccttcat
     2701 tggcccattg ctggcatgat ttgtcctgct tctgcctccg ccgaacagat caccgaaaat
     2761 atcaccgaaa atatcaccaa aatcagcgcc gctgaaatca aaaccgccga aaccgccggc
     2821 accgccgttt tcgaatgcag catgtccgaa ctggtcatat tgtctcttct tttccgggtc
     2881 gctcagtacg gcatatgcct ctgaggcttc tttaaacttt ttttcggcct ccgcattacc
     2941 cgggttcata tctggatgat atttttttgc cagtgtacga tatgccttct taactgtagc
     3001 ttcatcagca tttctatcaa cgccaagaac ctcataataa tctcttttcg actctgccat
     3061 aactgtcacc tttcgttact gttttatcgc aagatgactt cgcaaatctg cgaagccacc
     3121 caaaacattt taaactgtta aacttctttg taatctccat cgactacatc atcgccataa
     3181 ccggcattat caccgccgga tccctggctc atatccggcc ctgcctgacc gcccatatta
     3241 ctcatatccg gtcctgcccc ggatgcctgt gtctgttcat acatcttttc aaagagcttc
     3301 tgcgccgtct gcatcgcttt ttcctgcgct gccttcaatt caacgacctg ctcctccgtc
     3361 atattttcag gatctgcctt tgcaacaagt tcctttaagg aattcaattc tgcctctacg
     3421 gctgttttat catttgcatc aagcttatca ccgacttctt ccattgcctt ttcagtctgg
     3481 aaaatgaaag aatccgcatc gttctttgca tcgatcgctt ccttgcgctt cttatcctgt
     3541 gcttcaaatt cagctgcttc cctgactgcc ttgtcgatat cggaatctga catattggat
     3601 cctgctgtga tcgtaatatg ctgttctttt ccggtgccaa gatccttggc agatacattt
     3661 acaattccgt ttgcatcgat atcaaatgtt acttcgatct gcggcacacc gcgtcttgcc
     3721 ggcggaattc catccagacg gaactgaccg agagatttgt tgtcctttgc aaactgtctt
     3781 tcaccctgta cgacattgat atcgacagct gtctgattat cggccgctgt tgagaaaatc
     3841 tgactcttct tcgtcggaat cgttgtattt ctctcgatca gccttgttgc aacaccgccc
     3901 attgtttcaa tggaaagtga cagcggagta acatccagaa gaaggatctc acctgcaccc
     3961 gaatcgcctg caagcttgcc gccctgaatg gaagcaccga gcgccacgca ttcatccggg
     4021 ttcaatgatt tcgaaggttc ttttcctgta agctgtttta ctttatcctg aacagcaggt
     4081 atacgtgtgg aaccgccgac aagcagtacc tgtccgagtt cggatgacgt aagtcccgca
     4141 tcctttaatg cgctctgtac aggtccggtc gtagattcta caagatcatg tgtcagttca
     4201 tcaaatttag cccttgtcag attcatgtca aaatgcttcg gaccttctga agttgcagta
     4261 ataaacggaa ggttaatatt tgtcgttgtt gcagaggaaa gttctttctt tgctttttct
     4321 gctgcttcct tcaatctctg cagcgccatc ttgtcattgg acagatcaac gccttcgttt
     4381 ttcttaaact cggaaagcat ataattcgta atacgctggt caaaatcatc tccgccaagc
     4441 ctgttgttac cgcttgttgc caagacttcg ataacacctt cgccgatttc aataatggaa
     4501 acatcaaatg tgccgccgcc caggtcatat accatgatct tctgctcttt ttcattatca
     4561 agaccatatg ccagcgctgc tgccgtaggc tcattgataa tacgctttac atccaggcct
     4621 gcgatctttc ccgcatcctt tgttgcctgt ctctgtgcat cgttaaagta tgccggtacg
     4681 gtaataaccg cctccgtaac cttttctcca agatagcttt cagcatctgc tttcagcttc
     4741 tgcagaatca tcgcggaaat ctcctgcggt gaatattttt tactgtcgat cgttttttta
     4801 tgatccgtac ccatctctct cttaatggac gaaatcgttt tttccgcatt tgtaactgcc
     4861 tgacgttttg caggctctcc gacaagtctc tcccctgtct ttgtaaaggc cactacggac
     4921 ggtgttgtcc ttgcgccttc cgtatttgca ataacggtag gttttccgcc ttccataaca
     4981 gctacacagc tgtttgtcgt tcctaaatca ataccgataa ttttactcat gatcttatcc
     5041 tcctaatatt attataagtt ctctcatttt tcacattgat cgctttacgg tgctaaccga
     5101 caactccgac catgctgtgt ctgactacca tgtcacggta agtatatccc ttctgaagct
     5161 cctgtgcgac cgtaccgctt tcatactcct cactttccac ctgcatcacc gcattatgaa
     5221 aattcggatc aaactccttg ccgactgctt ctatctgctt tacccccagc tgctcaagtc
     5281 ctcctgacag ctgcttgtaa atcatattca ttccttcgac aaaagaagaa tctttctctt
     5341 cttcaggcac tgtctgcaga cccctttcaa aattgtcaat gaccggaagc agtttttcaa
     5401 ccacggattt tacacccatt tcatacatca atgacttttc tttttccgtc cgcttcctga
     5461 aattatcgaa ctccgccatc tgtcttttta ctttatctgt cagatcttcg atcatttcat
     5521 cttttttgtc ttttttctct ttcttatctt ttctgccaaa gtatttttta ttttcttctg
     5581 ctttttcttc tccggtttct tctccggttt ccgattcttc cgacttttca tcggccggtt
     5641 cttcttccgc ttccgactga ccagcttctg tctgaacagc ttctgcttct tcaccttccg
     5701 tccggatgct ttcttcggca tgctcctccg tcatatcttt cagaatatct tctgttaaat
     5761 ccttttccat ct
//
LOCUS       DAOSYE010000024         5762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9264_length_5762_cov_3.230419, whole genome shotgun sequence.
ACCESSION   DAOSYE010000024 DAOSYE010000000
VERSION     DAOSYE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9264_length_5762_cov_3.230419"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLU43_00820"
     CDS             complement(<1..843)
                     /locus_tag="PLU43_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOO26977.1"
                     /translation="MSRNLFKSSHVVVKSENKRVIDSNSLIAERIQTLSALLKEEELP
                     GDFPEEFSEGLDADQVERLLGDPAEQEADFREHEKSAEKLIQNAKEEAERILEAARED
                     ADGILKAAKAEAKSLKAEAIQAGHGEGYQAGYDEGLQGVEKMKAACVKEQKQLEDSYE
                     KRVNELEPQFVETLTGIYEHIFHLDLSDKKETVLYLLKDAIRNIESGKNFFAHVSKGD
                     YEFVSGRKEELLTGLGSAGTVEVIEDLTLQQGQCFIETEGGIFDCGIGTELELLKKEL
                     KLLSY"
     gene            complement(836..1861)
                     /gene="fliG"
                     /locus_tag="PLU43_00825"
     CDS             complement(836..1861)
                     /gene="fliG"
                     /locus_tag="PLU43_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOO26978.1"
                     /translation="MAGRTSREQSAVSGIQKAAILLIALGPERSALIFKYLKEEEIED
                     LTLEIANTRSVTPQVKESVIDEFYQVCLAQQYIAEGGIGYAKELLEKALGTDKAMDVI
                     GKLTASLQVKPFEFIRKTEASQLLNFIQDEHPQTIALILSYLSPSQAAMIVSALPPDR
                     QADVAKRIALMDRTSPDVIKEVEKILESKLSSLVNQDYTIIGGVDSVVEILNTVDRST
                     EKHIMETLEIDEPELADEIRKKMFVFEDILLLDDRAIQRVLRDVDNSDLAIALKSTNE
                     EVQNAIFNNLSKRLAVMIKEDMEFMGPVRMKDVEEAQQKIVNTIRRLEDSAEIVISRG
                     GGDEIVV"
     gene            complement(1868..3478)
                     /locus_tag="PLU43_00830"
     CDS             complement(1868..3478)
                     /locus_tag="PLU43_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOO26979.1"
                     /translation="MERLRKIPQQVLAWWNKFNAKQKTIIISVAAGVILTLAILVTIL
                     TRPQYEVLITCESTKEASEIIDLLDEDGIEYEVTDDGLIVSVKKEDISTATLLLGANN
                     YPADSYSLDAALGGEFSTTESDKQKTYKLYQEGHLEEIMEAQSAVRAAYVTLSIPDDD
                     GTLLATEEESYASVMLDLNEDLQVGVAENFAKNIATALGNKTTDNITIIDSNGNLLFS
                     GADSSETESSIVNTSNQIALKSEAERIVKQEVTQVLLGSNLYEDVKVAPNLVMDFSAV
                     NETEHTYTQADENGQNVLSHEETYESEGTNGSGGVPGTDTNTEEQTYVTDTGSNTTSS
                     VTQESRDYLPGEKITDKTVPPGAIQYDTSSIAITASHYVIYKESEVKAQGLLDGITFE
                     EFMQQNSDMVKKDVDEEMITLVANATGIPSTNISIIAYDVPIFQANDGSGLKLADIAQ
                     ILLILLILGLLGFVVFSGLRKEKKATEPEAELSVESLLQSTQEHQEIEDIDLESKSEV
                     RVMVEKFVDENPDAAAALLRNWLNEEWG"
     gene            complement(3520..3837)
                     /gene="fliE"
                     /locus_tag="PLU43_00835"
     CDS             complement(3520..3837)
                     /gene="fliE"
                     /locus_tag="PLU43_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOO26980.1"
                     /translation="MDISSLLNVSSDVISEAASKASTSTGLQTDDSFSTMFNTALGNL
                     NETNSLLNDSEEEKIKFALGITDNTHDLAIAAAKASTALSYTVALRDKFIEAYKEIMQ
                     MQI"
     gene            complement(3888..4337)
                     /gene="flgC"
                     /locus_tag="PLU43_00840"
     CDS             complement(3888..4337)
                     /gene="flgC"
                     /locus_tag="PLU43_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOO26981.1"
                     /translation="MSIFKSFGVNASGMTAERYRMDIISQNVANANTTRTEDGTPYKR
                     KVVTFEEKTLNPTEFGTIFAKSMGQDVGDGVKVSGVYEDNTEEMKKVYDPSHPDADED
                     GYVWYPNVNIVTEMTNMIDSSRAYEANATAFTASKTIAMKGLEIGRT"
     gene            complement(4371..4772)
                     /gene="flgB"
                     /locus_tag="PLU43_00845"
     CDS             complement(4371..4772)
                     /gene="flgB"
                     /locus_tag="PLU43_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOO26982.1"
                     /translation="MINTGVFNYVNVLDKAADGSWLKNEAISNNIANVDTPNYKRQDV
                     SFESELQQALKSSKYGTLDEKISNLNQGNNLSKIEAKTYTDSAEYSYRLDGNNVDIDN
                     ENVELASNQIKYNALVQAIDEDFINLKTVIK"
     gene            complement(5000..5761)
                     /gene="codY"
                     /locus_tag="PLU43_00850"
     CDS             complement(5000..5761)
                     /gene="codY"
                     /locus_tag="PLU43_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOO26983.1"
                     /translation="MLINRNINKLLHNNNSSKVVFNDICDVLKEILQSNVLVISKKGK
                     ILGTGAHKDIGEIEGLLKYEIGGIIDQMLNERLLGVLSTKENVNLETLGFELEDSSRY
                     QAIVTPIDIAGERLGTLFIYKVKEQYTIDDIILCEYGATVVGLEMLRAVNEESAEESR
                     KLQVVKSAISTLSFSEMEAITHIFDELSGNEGVLVASKIADRVGITRSVIVNALRKFE
                     SAGVIESRSSGMKGTYIKVLNPVIFEEIEMIKKNK"
BASE COUNT         1452 a         1539 c         1012 g         1759 t
ORIGIN      
        1 ataggacaaa agcttcagtt cctttttcag cagttccagc tccgtaccga taccacagtc
       61 aaaaataccg ccttctgtct caataaaaca ctgcccctgc tgcaaagtca ggtcttctat
      121 tacctcgacc gtaccggcac ttccaagtcc cgtcagaagt tcttcttttc tcccgcttac
      181 aaattcataa tcacccttgg atacatgcgc aaaaaaattt ttaccgcttt cgatattccg
      241 tattgcatct ttcaacagat acagaacagt ctccttttta tcggataagt ccaaatgaaa
      301 aatgtgttcg taaataccgg tcaaagtctc aacaaactgc ggttccagtt cattgaccct
      361 tttttcataa gaatcctcca gctgcttttg ttcttttacg caggcagctt tcatcttttc
      421 taccccttgt aatccctcgt cgtagccggc ctgatagcct tccccgtgtc cggcctgtat
      481 cgcctcggct tttaagcttt ttgcctccgc cttagctgcc ttgagaatac catctgcgtc
      541 ctcccgtgcc gcctcaagta tccgttctgc ttcttccttc gcattttgta tcaatttttc
      601 cgcagacttc tcatgctccc tgaaatcagc ctcctgctcc gcaggatcac caagaagacg
      661 ctctacctgg tctgcatcca gaccttccga aaactcctcc gggaaatccc ccggcagttc
      721 ttcttcctta agcagcgcac tcagtgtctg aatcctctca gcaatcagag agttggaatc
      781 aatcactcgt ttgttttcac ttttcactac cacatgactg gatttaaata aatttctaga
      841 caacgatctc atcacctccg ccgcgagaaa tcacaatttc agccgaatcc tcgagacgtc
      901 tgatcgtgtt gacgatcttc tgctgtgctt cttcaacatc tttcatacga acaggaccca
      961 taaattccat atcttccttg atcataacag caagacgttt ggaaaggtta ttaaagatcg
     1021 cattctgcac ctcttcattt gtgctcttca atgcaattgc aagatcactg ttatccacat
     1081 cacgcagtac acgctggata gctctgtcgt caagcagcaa aatatcttca aatacgaaca
     1141 tcttcttccg gatctcatcc gcaagctccg gttcgtcgat ttccaatgtt tccataatat
     1201 gcttttccgt actccggtct accgtattca aaatctcgac aaccgaatcc acgccgccga
     1261 taatcgtata atcctgattg acaagagacg aaagtttgga ttccaatatt ttttccacct
     1321 ctttaataac atccggcgac gtacggtcca tcagggcaat acgttttgca acatccgcct
     1381 gtctgtccgg cggaagagcc gaaacgatca ttgcagcctg gctgggactt aaataagata
     1441 aaatcaatgc aatcgtctgc ggatgctcat cctgtataaa attaagaagc tgggaagctt
     1501 ctgtctttct gataaattca aatggtttca cctgaaggga tgccgtcagc ttgccgatca
     1561 catccattgc cttatccgta ccaagcgcct tctccaaaag ttccttggca tacccgatac
     1621 cgccttccgc aatatactgc tgcgcgaggc agacctgata aaactcatca attaccgatt
     1681 cctttacctg cggtgtcacg cttcttgtat tggcgatttc aagggtcagg tcctctatct
     1741 cttcttcctt caaatattta aaaatcagcg cagacctttc cggtcctaaa gcaatcagca
     1801 gaattgcggc tttctggata ccggaaaccg cagattgttc tctgctcgtg cgtcctgcca
     1861 ttactcttta cccccattcc tcattcagcc agttacgaag caatgctgcc gccgcatccg
     1921 gattttcatc cacaaatttc tcaaccataa cccggacttc cgattttgat tccaaatcaa
     1981 tatcctcgat ctcctgatgc tcctgtgtgg actggagcaa tgattccaca gaaagctccg
     2041 cttccggttc cgtcgctttt ttctcttttc gcagtccgga aaacacgaca aaaccaagca
     2101 gccctaagat cagtaagatc aacaggatct gcgcaatgtc ggccaatttg agtcctgaac
     2161 cgtcgtttgc ctggaaaatc ggcacatcat atgcaatgat tgaaatattt gttgagggaa
     2221 tacctgtcgc attcgcgaca agtgtgatca tttcttcatc tacatccttc tttaccatat
     2281 cactgttctg ctgcataaac tcttcaaacg tgatcccgtc aagaagtccc tgcgccttta
     2341 cttcgctttc cttataaatc acataatgag aggccgtaat tgcaattgaa gaggtatcat
     2401 actgaattgc tccgggagga accgtcttgt cggtgatctt ctcacccggc agataatccc
     2461 ttgactcctg cgtaacactg gaagtggtat tggaacctgt atccgtgaca tacgtctgct
     2521 cttccgtatt cgtatcggtc cccggcacgc cgcccgagcc gttcgtgccc tcggattcat
     2581 aagtttcctc gtgggaaagc acgttctgtc cgttttcatc tgcctgtgta tacgtatgct
     2641 ccgtctcatt gaccgcagaa aaatccatga ccagattcgg tgcgactttc acatcctcat
     2701 agagattgga accgagcagt acctgcgtga cttcctgttt tacgatacgc tctgcttcag
     2761 atttcagcgc gatctgattg gaggtgttga caatgctcga ttccgtttcc gaggaatcgg
     2821 cgccggaaaa taataaattc ccgtttgaat caataatcgt aatgttatct gttgttttat
     2881 tgccaagtgc agtcgcaata tttttcgcaa aattttccgc aacccccacc tgcagatctt
     2941 catttaaatc cagcatgacg cttgcataag actcctcttc cgttgcaaga agcgtaccgt
     3001 cgtcatccgg aatagacaat gtaacatatg ctgccctgac cgcagactgt gcttccatga
     3061 tctcttccag atgcccttcc tgatagagct tataagtctt ttgtttatcc gattccgtag
     3121 tggaaaattc tcctccgaga gccgcatcca gactgtagct gtccgcagga taattattgg
     3181 cgccaagcag aagcgttgca gttgaaatat cctctttttt gacggaaacg atcagaccgt
     3241 catccgtcac ctcatattcg atgccgtcct catccagaag atcaatgatt tccgacgcct
     3301 ccttagtaga ttcgcacgta attaagacct catactgagg tcttgtaaga atcgtcacca
     3361 aaatggcaag tgtcagaata acacctgccg ccacactgat gataatcgtt ttctgttttg
     3421 cattaaattt attccaccac gctaaaacct gctgtggaat ctttctgagt ctttccactt
     3481 tcacatctcc gtctataaga ccagttaatt accgtaccat cagatctgca tctgcataat
     3541 ttctttataa gcctcaataa atttatcccg cagcgccacc gtataactga gtgctgttga
     3601 agcttttgcg gctgcaatcg ccaaatcatg tgtattgtcc gtgattccga gcgcaaattt
     3661 gatcttttcc tcttccgaat cattcaaaag actgttcgtc tcattcagat ttccaagcgc
     3721 cgtattaaac attgtactga aactgtcgtc ggtctgaagc cccgtacttg tcgaagcctt
     3781 ggaagctgcc tccgatatta cgtccgaact gacattcagc aatgaagata tgtccatatt
     3841 aattcctccc gccccaaatt attgactatg ccaatcatgc gatctcacta tgtcctgccg
     3901 atctcaagtc ctttcatcgc gatcgtcttg cttgccgtaa acgctgtcgc gtttgcttcg
     3961 tatgcccgcg aagaatcgat catatttgtc atctccgtta cgatattgac attcggatac
     4021 cagacataac catcttcatc cgcatccgga tgggaagggt cataaacctt tttcatttct
     4081 tccgtattat cttcatatac gccggaaacc ttaaccccat ccccgacatc ctgtcccatg
     4141 ctctttgcaa aaatcgtgcc gaattccgtc ggattaagtg ttttctcttc aaacgtcaca
     4201 accttgcgtt tataaggtgt cccgtcttct gttcgtgttg tattggcatt ggccacattc
     4261 tgtgagataa tatccatccg gtatctctca gccgtcatac cggatgcatt cacgccaaat
     4321 gacttgaaaa tactcataat gatccaccgt cctctctgtc tgccaaacca ctacttaatt
     4381 acagtcttca gattaataaa atcttcatcg atcgcctgca caagcgcatt atatttgatc
     4441 tgattggacg caagctctac attttcatta tcgatatcga cattattgcc gtcaagacga
     4501 tacgaatatt ccgcagaatc cgtataagtt ttcgcttcaa tttttgacag gttgttaccc
     4561 tggttaagat tggagatctt ttcatccaag gttccgtact ttgaagattt caaggcctgt
     4621 tgtaattccg attcaaatga cacatcctgc cgtttgtaat tgggcgtatc gacattcgca
     4681 atattattgg aaattgcttc gtttttaagc caggagccgt ccgccgcctt gtcaagtaca
     4741 ttgacataat taaatactcc cgtatttatc atattcttca cctaccttac tatattataa
     4801 attatttgta gaaaaacaca agaacttttt caaaaaaaac cactttatat tgtgtatttt
     4861 actatttatg catacaagat gatggatgtt atgtaaatca atgttctaaa taaaacggat
     4921 ctatcagccg ctgttacgtc agataaatcc gtttatccat gctttataac tccgtttaca
     4981 aagcctcttt attcttttgt cacttatttt tcttgatcat ttcgatttct tcaaaaataa
     5041 ccggattaag cactttgata taagttccct tcattccgga agatcgggat tcgatcacac
     5101 cggcgctttc aaacttgcga agggcattta caatcacaga acgggtgatt cctaccctgt
     5161 ccgcgatctt gctcgcaaca agcacacctt catttccgct cagctcatca aagatatgcg
     5221 tgatcgcttc catctcggaa aatgacagcg tactgatcgc cgacttgact acctgcagct
     5281 tccggctctc ttccgcactc tcttcattaa ctgcccgcag catctccaat ccgacaactg
     5341 tcgctccata ttcacataat ataatatcgt cgattgtata ctgttcctta accttataaa
     5401 taaataatgt tccaagacgt tcccctgcaa tatcgatcgg cgtcacaatt gcctgatacc
     5461 tgctgctgtc ctccagttca aaaccaagcg tttccaggtt gacattctct tttgtggata
     5521 atacgcctaa aagacgttcg ttcaacatct ggtcaataat gccgccgatt tcatatttta
     5581 aaagaccctc gatctctccg atatccttat gcgctccggt tccgagaatc ttgccctttt
     5641 tgctgatcac aagtacgttc gactgaagaa tctcctttaa cacatcacaa atatcattaa
     5701 aaaccacctt gctggaatta ttattatgca acagtttgtt gatgttcctg tttataagta
     5761 ta
//
LOCUS       DAOSYE010000025         5716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9356_length_5716_cov_3.474474, whole genome shotgun sequence.
ACCESSION   DAOSYE010000025 DAOSYE010000000
VERSION     DAOSYE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_5716_cov_3.474474"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLU43_00855"
     CDS             complement(<1..816)
                     /locus_tag="PLU43_00855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO26984.1"
                     /translation="MTVESNEIEDWPEGPLLGAESAILMDADTGVVLYEKNVHEQLYP
                     ASTTKLLTALLAIENSSMDDIVTFSHDAIFNIDQDSSRIGIDVGEQLTMEQCLYGLLL
                     GSANEVAYAIAEHVGGDMETFIDMMNARAVSLGCTDTHFANANGLPESDHYTSAYDLA
                     LIARACFQNETLAKISGTSHYTIPPTNIQSEERCLENHHQMLSGEKYAYDGIIGGKTG
                     YTNDARQTLVTCAERDGIRLICVIMKEESPDQFLDTAALFDYGFDNFQKLNISK"
     gene            1143..1811
                     /gene="trmB"
                     /locus_tag="PLU43_00860"
     CDS             1143..1811
                     /gene="trmB"
                     /locus_tag="PLU43_00860"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOO26985.1"
                     /translation="MRLRNITGSRELIAENRFVIHAPEKIKGNWRSEVFQNQNPLQIE
                     IGMGKGQFIMKLAEKNPQINYIGIEKYSSVLLRAIQKMEDKQEELKNLRFIRMDANTI
                     TDIFESREVDKIYLNFSDPWPKDRHAKRRLPSKEFLACYRKILKDGAKLEFKTDNRQL
                     FDFAMNEAPAAGWQIEQYTYDLHHEPEMAKDNIMTEYEEKFSSAGNPICKYVISYRNA
                     SFLF"
     gene            complement(1797..2885)
                     /locus_tag="PLU43_00865"
     CDS             complement(1797..2885)
                     /locus_tag="PLU43_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOO26986.1"
                     /translation="METKTRLDYFDMAKGIGMILVLLGHLQGDAIFSYSPYILPLCTW
                     IFSFHMPLFFIISGMLMNYKHDLQKDMAVLCKKRFRGIMIPYFWFSLFYISVVFYALF
                     FGGSIAPETLYVNLWYIFSMYGMNVLWFLPALFFGELLFLFLTKKLGTKKSIAAIVIL
                     TILASAASCWLQTLHYETPLQERLHEFSLVLLRPILACTFICIGYYVFYLFKERERFC
                     MKELLLGILFLGIGIAFTHVNHGVDFRSLVQKNLFFYYLCALSGSFGLILVCKNIKPL
                     GLIKFWGINSLAFMAIHNNKTILYSGMQCAMYLNQYITRARGYISYITVILIILIYVT
                     LMIWIINRFFGFIVGKPSPVTDLLLKKK"
     gene            complement(2905..3978)
                     /locus_tag="PLU43_00870"
     CDS             complement(2905..3978)
                     /locus_tag="PLU43_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOO26987.1"
                     /translation="MDMNTKRLTYFDMAKGIGIILVVLGHIEYISEPLRAWISSFHMP
                     LFFIVSGMLIHYKDETSKDLRKTLQKKWHSIIIPYFWFSLLYFLIDILNVILHKIDLH
                     TFLTDQIHSISFYGISVLWFLPALFIAESGFLFFTKKLPRILSICAILFTALLSYFIQ
                     IGISRIYTAYETSLLITSLIDFVRVFLRGAIAMSFVCIAYYLFYLLKRNDGFSIKELL
                     LGIVLFGLNIPLSQVNTCVDFHYIILKNVPLFYICALSGSAGLILLCKNIKNIRLLGY
                     FGKNSLIIMSTHVNCYILYAAILIAWQIDTFVTRAKSYVFLFNIMLFTFLFEIVLIEL
                     INRFFPFILGKKRTKQAVCREKK"
     gene            4212..4532
                     /locus_tag="PLU43_00875"
     CDS             4212..4532
                     /locus_tag="PLU43_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26988.1"
                     /translation="MDVMISKLSEIELAATSIVENAKQKKDDIFRETDEKKKDIDKDA
                     EEEIVQRIEKLKKEMEEKKEKDITELKKCVQSSIRNISDTYNNQHEQIAGSIAERIIK
                     NVQP"
     gene            4562..5629
                     /locus_tag="PLU43_00880"
     CDS             4562..5629
                     /locus_tag="PLU43_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOO26989.1"
                     /translation="MSGLKTYSGISTKIRAKQKKMFHQGTYDRLTASKSVQEVLRILN
                     ESEGYHNLFAGKDLNSLHRGAIEQILKECYYRDYISLYKFSDEKQRVFLKMYFRKLEL
                     SFLKACLRNLLNKDKEYEKLTISEDVGRLFLKYSKLDMELLIKASTIDNLIDALKGTE
                     YYRSLKTVHEQGGNSLFEYEVSMDLYYFTRLWKMINQQFSGLDLEILIRSYGYKIDIL
                     NMQWIYRCKKYYKVTTADICAMVIPINYKLHKEELTNLIESANIEEFDRIMKSTAYGK
                     LYPDISGDNLEEIYIYLLDKINDTDSDRYPYSIAIINTYLYKKEYEVNKITTILECIR
                     YGIKYDKILQYSGLKGGAVRD"
BASE COUNT         1971 a          996 c         1211 g         1538 t
ORIGIN      
        1 ttttgaaata ttcagtttct ggaagttatc aaatccataa tcaaataatg cagccgtgtc
       61 taaaaactga tcagggcttt cttctttcat aataacgcag atcagacgga tgccgtctct
      121 ttccgcacag gttacaagtg tttgcctggc atcgtttgta tacccggttt ttcctccgat
      181 gatcccgtca tacgcgtatt tttctccgga aagcatttga tgatggtttt caagacaacg
      241 ttcttctgac tggatatttg tcggcggaat cgtataatgt gaggtaccgg atattttagc
      301 cagcgtttca ttttgaaaac aggcgcgtgc aattaatgca agatcatagg cagacgtata
      361 atgatccgat tccggcaggc cgtttgcatt tgcaaaatgt gtatctgtac agccaagcga
      421 aacagcacgc gcattcatca tatcgatgaa ggtttccata tcaccgccga catgttccgc
      481 tattgcatag gcgacttcat ttgccgaacc taaaagaagg ccatacaggc actgttccat
      541 ggtcagctgt tccccaacat cgattccaat acggctggaa tcctggtcaa tgttaaaaat
      601 tgcatcatgc gaaaaggtga cgatgtcgtc catcgatgaa ttttcgatcg ccagaagcgc
      661 ggtcagcagc tttgtcgtac ttgcaggata aagctgttca tgtacatttt tttcataaag
      721 aacaacgccg gtatcggcgt ccatcaagat agcggattcc gcaccaagca aaggaccctc
      781 cggccagtct tcaatttcat tactttctac agtcatggtt tttctttctt ctgcctgctg
      841 cgttaaatcg tctgttggaa ccgtattaac ggcaaaaacc ggaaggctgt taaaaagtat
      901 agctataaaa cacagaaaaa cacctgttaa tttttttaaa gaaaagcgtc tcatgtaaac
      961 tcctgtataa ttatgataat gtgacagaaa aagtacctta tttatcatac aacattccga
     1021 attaaatgcc aaaggatttt ctgtgaacat tcttttttgc gttttgcaaa cagcttgtca
     1081 gaaaacaggt tttatattat actgattctg ttatgaaaac aggaactgtt acggagaaaa
     1141 ctatgcgttt aagaaatatt acaggatcac gggaattgat tgcggaaaat cgttttgtca
     1201 ttcatgcacc tgaaaaaata aagggaaatt ggcggtccga agtttttcag aatcagaacc
     1261 ccctgcaaat agaaattggt atgggaaagg gtcagtttat tatgaagctg gctgaaaaaa
     1321 atccgcagat caattatatt ggaattgaaa aatattccag tgttttactt cgggcaattc
     1381 aaaaaatgga agataagcag gaagagctga aaaatctgag atttatccga atggatgcga
     1441 atacgattac agatattttt gaaagcaggg aagtggataa aatttattta aatttttccg
     1501 atccatggcc gaaagaccgg catgcgaaac gccgccttcc gtccaaagaa tttttagcct
     1561 gttatagaaa gatcttaaaa gacggcgcaa agctggaatt taaaacagac aacaggcagc
     1621 tgtttgattt tgcaatgaat gaagcgcctg cagccggatg gcagatagag cagtatacgt
     1681 atgacctgca tcatgagccg gaaatggcaa aagataatat tatgacagaa tatgaagaga
     1741 aattctcttc agccgggaat ccgatctgca aatacgtgat cagttacagg aatgcatcat
     1801 ttctttttta acagaagatc cgtaacagga gatggttttc caacgatgaa cccaaaaaac
     1861 ctgtttatga tccagatcat gagcgtcaca tagatcaaaa taatcagaat caccgttatg
     1921 tagctgatat aaccgcgggc acgggtaata tactggttca gatacatggc gcactgcatt
     1981 ccggagtaaa gaatcgtttt attattatgt atggccataa atgcaaggga attgatgccc
     2041 cagaatttga tcagccccag cggtttgata tttttacaga ccagaatcag tccgaaggaa
     2101 ccggaaagtg cacataaata ataaaagaaa aggttttttt gaacaagaga acgaaaatca
     2161 acaccatgat tgacgtgagt aaaggcaatt ccaatgccta ggaagagaat tccaagaagt
     2221 aattctttca tacagaatcg ttctctttcc ttaaacagat aaaacacata atagccgata
     2281 cagataaacg tacatgccag aatcgggcgc agaagcacaa gagaaaattc atgcagtctt
     2341 tcctgcagag gtgtttcgta atgcagtgtc tgcagccagc agctggctgc tgatgcaagg
     2401 atcgtcagaa ttactatggc tgcgatcgat tttttcgttc cgagtttttt tgtcaggaac
     2461 agaaacagaa gctcgccgaa aaataatgcc ggcagaaacc aaaggacatt catgccgtac
     2521 atactgaaaa tataccatag atttacatac agcgtttccg gtgcaataga gccgccaaaa
     2581 aataatgcat agaatacgac actgatataa aacaggctga accaaaaata gggaatcata
     2641 attcccctga atcgtttttt acataaaaca gccatatcct tttgaagatc gtgtttataa
     2701 ttcatcagca taccggatat aataaaaaac aatggcatgt gaaaagaaaa gatccatgta
     2761 caaagaggca gaatataggg agaatagcta aaaatagcat ctccctgcaa atgtcctaaa
     2821 aggacaagga tcatgccaat gccttttgcc atatcaaagt aatcaagtcg cgttttggtt
     2881 tccataatac ctccggtgtc cggtttattt tttttctctg cagacggcct gtttcgttcg
     2941 tttttttcca aggataaacg ggaagaaacg attaatgagc tctatcagaa cgatttcaaa
     3001 gagaaaggta aacagcataa tattgaataa aaaaacatag ctttttgctc ttgtcacaaa
     3061 agtgtcgatc tgccaggcga tcaggatggc agcataaaga atatagcagt tgacatgggt
     3121 cgacatgata atgagtgaat ttttgccgaa atatccgagc aatcggatat tttttatatt
     3181 tttacagagc aggatcaggc cggcggagcc ggacagggcg cagatgtaaa acagaggtac
     3241 gttttttaaa ataatgtaat gaaaatccac acaggtgttg acctgtgaaa gagggatatt
     3301 cagaccgaaa agaacaattc caagcaaaag ctcctttatc gagaatccat cgtttcgctt
     3361 taacagatag aacagataat aggcgatgca gacaaaagac attgcgatcg caccgcgcag
     3421 aaagactctg acaaagtcga tcagggaagt gatcagcagt gatgtttcat atgcggtata
     3481 tatccggctg attccgatct gtataaaata ggaaagcagg gctgtaaaaa ggatggcgca
     3541 aatggaaagg atccgcggca gcttcttcgt aaaaaaaaga aaaccggatt ctgcaataaa
     3601 taaggcaggc agaaaccata aaacggaaat accgtaaaag cttatggagt gaatttggtc
     3661 tgtaagaaat gtatgcagat caattttatg caggatcaca tttaaaatat cgatcagaaa
     3721 ataaagcagg ctgaaccaga agtagggaat gataatggaa tgccattttt tttgaagcgt
     3781 tttgcgcaga tcttttgagg tttcgtcctt ataatggatc agcatgccgg atacaatgaa
     3841 aaacagcggc atatgaaagg aagagatcca tgctctgagc ggttcactga tatattcgat
     3901 atgtccgagt acgacaagaa taataccaat accctttgcc atatcgaaat aagttaagcg
     3961 ttttgtattc atatccatag tataaaatct ccgtaagtga aaattccatg aatgtaaaat
     4021 gtttttaata gtactttatt tatggaatta tttaagttct attttagaag agattagaaa
     4081 aaaagacaag aaaaaataag aaaataaaca caagggaaat ttttacattg aaattttact
     4141 tatttcaggc tataatcagt tatatttacc tattctaaat gagagagttt tcagaaaaag
     4201 agggaattgt catggatgtt atgatttcaa agctatccga aatagaactg gccgcaacaa
     4261 gcattgttga gaatgccaaa cagaaaaagg atgatatctt tcgagaaaca gacgaaaaga
     4321 aaaaagatat agataaagat gcagaagaag aaattgttca gaggatcgaa aaattaaaaa
     4381 aagaaatgga agaaaaaaag gaaaaagaca ttacggaact gaaaaaatgt gtacaaagct
     4441 ccatacggaa catttcggat acttataaca atcagcatga acagatagcc ggcagtatag
     4501 cggagcggat tattaaaaat gttcagccct gatgcagggg gaacaggaag gtgaagcgtc
     4561 tatgagcgga ttaaagactt atagcggaat ttctacaaaa atccgggcaa aacaaaagaa
     4621 aatgtttcat cagggaacat atgacaggct cacggcttca aaatcagtac aggaagtttt
     4681 acgtattttg aatgaaagcg aaggttacca caatcttttt gcaggcaaag atttgaattc
     4741 ccttcacaga ggtgcgatcg aacagatcct gaaggaatgt tattacaggg attatatcag
     4801 tctttataaa ttttccgatg aaaaacagag agttttttta aaaatgtatt ttcgcaagct
     4861 ggaattaagc tttttaaagg cgtgcctgag aaatctgctt aataaggata aggagtatga
     4921 aaaactgacg atttccgaag atgtaggcag gctttttttg aaatactcca aactggatat
     4981 ggaacttctt ataaaggctt ctacgatcga taatctgatc gacgcgctga aagggacgga
     5041 atattacaga tcattgaaaa cagttcatga gcagggcgga aactctttgt ttgaatatga
     5101 agtctccatg gatctgtatt attttacaag gctctggaaa atgatcaatc aacagttcag
     5161 cggcctggat cttgagatcc tgatcagaag ttatggatat aaaattgata ttttaaatat
     5221 gcagtggatc tatcgctgta aaaaatatta taaggttaca actgccgata tctgtgcaat
     5281 ggtgataccg atcaattata agcttcacaa ggaagagctg acaaacctga tagaatccgc
     5341 caatatcgaa gaatttgacc ggatcatgaa aagtacggcg tacggaaagc tatatccgga
     5401 tatttcaggc gataatttgg aagaaatata tatttatctg ctggataaga tcaatgatac
     5461 ggattccgac agatatccgt attcgattgc gattatcaat acctatttat ataaaaaaga
     5521 atatgaggtc aataagatta cgaccatact ggaatgtatc cgctatggta taaagtatga
     5581 taaaatcctg cagtattcgg gactgaaagg aggagcggtg cgtgattgag catatgagtt
     5641 tcatcaatat tatgggtccg aaggatgatt ttgaccgggt agtccagaat tatctgagaa
     5701 agtatgacat gcagct
//
LOCUS       DAOSYE010000026         5711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9370_length_5711_cov_2.917610, whole genome shotgun sequence.
ACCESSION   DAOSYE010000026 DAOSYE010000000
VERSION     DAOSYE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9370_length_5711_cov_2.917610"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLU43_00885"
     CDS             <1..585
                     /locus_tag="PLU43_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO26990.1"
                     /translation="VPGPVQDWKLILVNKQNPVPDDYEMELVSINGSMQVDKRIAKEL
                     AKMLEAAKNDDVSLMICSAYRSYERQTTLFENKIRKSMKSGMSYLDAYAEASYSVTVP
                     GTSEHQLGLALDIVTPSYTTLDKGYENTDAGKWLKAHSSAYGFILRYPEGKEHITGIT
                     YEPWHFRYVGKQAAEIITENGLTLEEYLKQNGNN"
     gene            587..1717
                     /gene="tgt"
                     /locus_tag="PLU43_00890"
     CDS             587..1717
                     /gene="tgt"
                     /locus_tag="PLU43_00890"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOO26991.1"
                     /translation="MYQILHTEGRAKRAQFHTVHGTIQTPVFMNVGTAAAIKGAVSTE
                     DLLQLKTQVELSNTYHLHVRPGDEIIKKLGGLHKFMVWDKPILTDSGGFQVFSLSSLR
                     KIKEEGVYFHSHVDGRKIFMGPEESMRIQSNLASTIAMAFDECPSAKAERAYVQASVD
                     RTTRWLERCQTELHRLNSLEDTINQNQLLFGINQGAVFSDIRIEHAKQISELDLDGYA
                     VGGLAVGETHEEMYHILEEVVPYLPQNKPTYLMGVGTPANILEAVERGVDFFDCVYPT
                     RNGRHGHLYTNHGKLNLFNAKYELDDRPIEEGCGCPTCRRYSRAYIRHLLKAKEMLGM
                     RLCVLHNLYFYNTMMEEIRTALEQGCFAAYKRKKLENMENGI"
     gene            1833..2147
                     /gene="yajC"
                     /locus_tag="PLU43_00895"
     CDS             1833..2147
                     /gene="yajC"
                     /locus_tag="PLU43_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOO26992.1"
                     /translation="MYTFLTETTASSTANIFMIVLYVVVIGGFVYFAMIRPQKKEQSR
                     MNAMLSELEAGDSVLTSSGFYGVVIDITDDTVIVEFGNNKNCRIPMQKTAITQIEKPS
                     AE"
     gene            2315..3397
                     /gene="leuB"
                     /locus_tag="PLU43_00900"
     CDS             2315..3397
                     /gene="leuB"
                     /locus_tag="PLU43_00900"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOO26993.1"
                     /translation="MNYKIACIPGDGIGPEIVAQAKKVLDKVAEKYGHTFIYMDLLMG
                     GASIDIHGVPLTDDTINQAKASDAVLMGSIGGNTSTSPWYKLAPELRPEAGLLALRKE
                     LHLFANLRPAILYNELSAACPLKEEIIGDGFDMVIVRELTGGLYFGERKTIEENGIKK
                     AIDTLTYDENEIRRIAIKGFETAMKRRKKVTSVDKANVLDSSRLWRSVVNEVSEEYPE
                     VTLEHMLVDNCAMQLVRDPAQFDVILTENMFGDILSDEASMVTGSIGMLASASLNDTS
                     FGLFEPSHGSAPDIAGMDKANPIATILSASMLLRYSLGLEKEADAIDASVKQVLSEGF
                     RTGDIMSEHCRLVGTKEMGTQIAERI"
     gene            3429..5096
                     /gene="ilvD"
                     /locus_tag="PLU43_00905"
     CDS             3429..5096
                     /gene="ilvD"
                     /locus_tag="PLU43_00905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOO26994.1"
                     /translation="MKSDAVKTGTQQAPHRSLFNALGFTKEEMERPMIGVVSSYNEIV
                     PGHINIDKIVEAVKMGVAMAGGTPVVFPAIAVCDGIAMGHSGMKYSLVTRDLIADSTE
                     CMALAHQFDALVMVPNCDKNVPGLLMAAARVNIPTVFVSGGPMLAGHVHGKKTSLSSM
                     FEAVGAVSAGKMTEEELEEYEEKVCPTCGSCSGMYTANSMNCLTEALGMGLKGNGTIP
                     AVYSERIKLAKHAGMAVMEMYRKNICPRDIITKDAVLNALTVDMALGCSTNSMLHLPA
                     IAHEIGFDFDIAIANEISAKTPNLCHLAPAGPAYIEDLYEAGGVYAVMKELADIGLLN
                     TDCMTVSGKTIGENIAGAVNKNPEVIRTVDHPYSKTGGLAVLKGNLAPDGSVVKRSAV
                     VEEMMVHEGPARVFDCEEDAIAAIKSGKIVEGDVVVIRYEGPKGGPGMREMLNPTSAI
                     AGMGLGSSVALITDGRFSGASRGASIGHVSPEAAVCGPIALVKEGDMIKINIPENQLN
                     FDISEEELAKRKAEWQPRKPKITSGYLSRYERLVTSGDKGAVLKNPE"
     gene            5144..>5711
                     /locus_tag="PLU43_00910"
     CDS             5144..>5711
                     /locus_tag="PLU43_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOO26995.1"
                     /translation="MLLTGAQIVMECLKEQGVDTVFGYPGGTILNIYDELYKYQDTIK
                     HYLTSHEQGAAHAADGYARATGKVGVCMATSGPGATNLVTGIATAYMDSVPMVAITAN
                     VILPLLGKDTFQEVDIAGVTMPITKHGYIVKDVKVLADTIRKAFVIARSGRPGPVLVD
                     ITKDVTADKTEYTPVKPVLLPSPSGYTKK"
BASE COUNT         1721 a         1090 c         1480 g         1420 t
ORIGIN      
        1 gtaccgggtc ctgtacagga ctggaagctg attcttgtca ataagcagaa tcccgtaccg
       61 gacgattatg agatggaact ggtgtccatt aacggcagta tgcaggtcga caaacgaatc
      121 gcaaaagagc ttgccaaaat gcttgaggcc gcaaagaacg acgatgtcag tctgatgatc
      181 tgttctgcat acagaagcta tgaaagacag acgacattat ttgaaaataa gatacggaaa
      241 tccatgaaaa gcggaatgtc ttatctggat gcctatgcgg aagcttccta ttccgtgacg
      301 gttccgggaa caagtgagca tcagctcgga cttgcgctgg atattgtaac cccttcctat
      361 acgacgctgg ataagggtta tgaaaatacg gatgcaggaa aatggctgaa agcgcattct
      421 tctgcatatg gatttatttt gcgttatccg gaaggaaaag agcatattac cggaatcacc
      481 tatgaaccgt ggcatttcag atatgtcgga aagcaggcgg ctgaaattat tacggagaat
      541 ggtctgacgc tcgaagaata tttgaaacag aatggaaata actagcatgt atcagatttt
      601 acatacggaa ggcagagcaa aacgcgcaca attccatacg gttcacggaa ccattcagac
      661 accggtattt atgaatgtcg gtacggctgc tgcaatcaaa ggggcagtgt cgacagaaga
      721 tctgttacag ttaaaaacgc aggtggagct ttccaacaca tatcacctgc atgtacgtcc
      781 gggcgatgaa atcataaaaa aacttggggg tcttcataaa tttatggtat gggataaacc
      841 gatactgacc gactcgggtg gttttcaggt gttttcctta agcagtctgc gcaagatcaa
      901 agaagaaggt gtttattttc attcccatgt ggacggaaga aaaatattca tgggtcctga
      961 ggaaagtatg aggatccagt caaaccttgc ttccacgatc gcaatggcgt ttgatgaatg
     1021 tccgagtgcg aaagccgaac gggcttatgt gcaggcttcc gttgacagaa caacaagatg
     1081 gctggaacgc tgccaaacag aactgcatcg tctaaacagt cttgaagata cgatcaatca
     1141 gaaccagctg ctgtttggaa tcaatcaggg ggcagtgttt tctgatatcc gtatcgaaca
     1201 tgcaaaacag atttcagagc ttgatcttga cggttatgcc gtaggcggcc tggccgtagg
     1261 ggaaacgcac gaggaaatgt accatattct ggaggaagtt gttccatacc ttcctcaaaa
     1321 taaaccgacg tatttaatgg gcgtcggaac accggccaat atattggaag cggttgaacg
     1381 cggcgtcgac ttttttgact gtgtttatcc gacacgcaat gggcgtcacg ggcatctcta
     1441 tacaaatcac ggtaaactga atctgtttaa tgcaaaatat gaactggatg accgtccgat
     1501 agaagaaggc tgcggctgtc cgacatgcag gcgatattcc agggcttata tcagacatct
     1561 tttgaaagca aaggaaatgc tcggcatgag gctttgcgtt cttcataatc tttattttta
     1621 caatacgatg atggaagaga tcaggacagc actcgaacag ggctgctttg ccgcatacaa
     1681 acggaagaag cttgaaaata tggaaaacgg aatataatct ataaccacag gtaatattga
     1741 ttaaaatatt cataaaatac ttgatgtatg aacaaatatt gtgtatgata taaaagcaca
     1801 ggtttttaga aagtatattc aggaggaatc atatgtatac atttttaacg gaaacgacgg
     1861 cttcttcaac agccaatatt tttatgatcg ttctttatgt ggtagtaatc ggtggttttg
     1921 tgtattttgc catgatccgt ccgcagaaga aagagcagtc aagaatgaat gcaatgcttt
     1981 cagagctcga ggctggagac agtgttttga catcaagcgg tttttacggc gttgtcattg
     2041 atattacgga cgataccgtt atcgtggagt tcggaaacaa taaaaactgc aggattccaa
     2101 tgcagaagac tgcaattacc cagattgaaa aaccgtctgc agaataaatc tgaaaagttt
     2161 atccgatgat acgatagaca aaatgtggaa aatagcggga tgctccaatc ggagcatctt
     2221 gctttatata tttcgataca aaaaataatt ggtgccaaat aaaattattt ggtcatgcca
     2281 tttataaaca atagaactta taggagtgat gaaaatgaat tacaaaatag cctgtattcc
     2341 gggcgacggt atcggaccgg aaattgttgc ccaagcaaaa aaagtgcttg ataaggtggc
     2401 agaaaaatat ggtcatacat ttatttatat ggatctgtta atggggggcg cttccattga
     2461 tatacatggt gtgccgctga cagatgacac gattaatcag gcgaaagctt ccgacgcggt
     2521 attgatgggg tcgatcggcg ggaatacttc cacatccccc tggtataagc tggcacctga
     2581 attacgaccg gaagcaggac tgctggcact tagaaaagag cttcatttat ttgctaattt
     2641 aagacctgcc attttatata acgaattatc ggcagcctgt ccgctgaaag aggaaattat
     2701 cggagacggg tttgatatgg tgatcgtcag ggaactgaca ggcgggcttt atttcggaga
     2761 acgaaaaaca attgaagaaa atgggattaa aaaagcgatc gatacattga catatgatga
     2821 gaatgagatt cgcagaatcg ccataaaagg atttgaaacc gcaatgaaac gcaggaaaaa
     2881 agtcaccagt gtcgataagg caaatgtttt ggattcctcc agactttgga gaagtgttgt
     2941 caacgaggtg tctgaagagt atccggaagt gacgctggag catatgcttg ttgacaattg
     3001 tgcaatgcag cttgtcaggg atcccgcgca gtttgatgtg attctgacgg aaaatatgtt
     3061 tggagatatt ttatccgatg aagcgagtat ggtgacgggt tcgatcggta tgcttgcttc
     3121 cgcaagctta aatgatacaa gttttggact gtttgaacca agtcatggtt cagcgccgga
     3181 tattgcaggt atggataaag cgaatccgat cgctacgatc ctgtcggctt cgatgctgct
     3241 gagatattct ctcggactgg aaaaagaagc ggatgcgatc gacgcctccg taaaacaggt
     3301 cctttctgag ggctttcgga caggtgacat tatgtccgaa cattgcagac ttgtcggcac
     3361 aaaggaaatg ggcacacaaa tagcagagag aatctgaaca aaatcaaata aggaaagagg
     3421 gaaatatgat gaaaagtgat gcggtaaaaa caggaaccca gcaggctccg cacagatcgt
     3481 tgtttaatgc acttggtttt acaaaagaag aaatggaacg gccgatgatc ggtgttgtca
     3541 gttcttacaa tgagatcgtt ccgggacata tcaatataga taagatcgta gaggccgtca
     3601 aaatgggggt ggccatggca ggcggaacgc cggtcgtatt tccggcgatc gcagtctgcg
     3661 acgggattgc aatgggacac agcggtatga agtattcgct tgttacaaga gatctgattg
     3721 ccgattcaac agaatgcatg gcacttgccc atcagtttga cgcgcttgtt atggtgccga
     3781 attgcgataa aaatgtaccg ggacttttaa tggcagcagc aagagttaat ataccgaccg
     3841 tatttgtatc cggaggtcct atgctggcag gacatgtaca cggaaaaaag acaagtctgt
     3901 ccagtatgtt tgaagcagtc ggagccgtaa gtgcaggcaa aatgactgag gaagaattag
     3961 aggaatatga agaaaaggtc tgtccgacat gcggttcctg ttccggtatg tatacggcaa
     4021 acagcatgaa ctgcctgacg gaagcactcg gaatgggact gaaaggaaac ggtacgatcc
     4081 cggcagttta ttctgaacgg atcaagcttg caaaacatgc cggcatggcg gttatggaaa
     4141 tgtaccgtaa aaatatctgc ccgcgtgata ttattacaaa agatgcggtt ctgaatgcgc
     4201 tgaccgtaga tatggctctt ggatgttcta caaattccat gctgcatctg cctgcgatcg
     4261 cacatgagat cggttttgat tttgatattg ccattgcaaa tgaaatcagt gcaaagaccc
     4321 ctaatttatg ccatcttgcc ccggcaggtc cggcctatat cgaagatctg tatgaagcag
     4381 gcggtgttta tgcagttatg aaggaattgg ccgatatcgg tcttctcaac acggactgca
     4441 tgacggtatc aggtaaaacg atcggtgaaa atatcgccgg tgcagtcaat aaaaatccgg
     4501 aagtgatcag aacggtggat catccgtaca gcaagaccgg aggcctggcc gtattgaaag
     4561 gaaatctggc accggacggc agtgttgtaa aacgttccgc agttgtggaa gaaatgatgg
     4621 tgcatgaagg accggcaagg gtatttgact gtgaagagga tgccattgct gcgatcaaga
     4681 gcggtaaaat cgttgaggga gatgttgtcg tgatccgtta tgaaggacca aagggtggtc
     4741 ctggtatgcg ggaaatgctg aatccgacat cggcgatcgc cggtatggga cttggctcca
     4801 gtgttgccct gattacggac ggccgttttt caggagcttc ccgcggtgct tccatcggcc
     4861 atgtttcacc ggaggcagct gtctgcggac cgatcgctct tgtaaaggaa ggagatatga
     4921 ttaagatcaa tattcctgaa aatcaattaa attttgatat ttccgaagaa gagcttgcga
     4981 aaagaaaagc ggaatggcag ccgagaaagc caaagatcac aagcggatat ctgtccagat
     5041 atgaaaggct tgttacaagc ggcgacaagg gtgcagtact caaaaatccg gaataaaaat
     5101 tatgacagga actttatata ataatcagat aggagtgtaa actatgctgt tgacgggtgc
     5161 gcagattgta atggaatgct tgaaagagca gggagtcgat accgtattcg gttatccggg
     5221 cggtacgatt ttaaatatat atgacgaatt gtataaatac caggacacga tcaaacatta
     5281 tctgacctca cacgaacagg gtgccgctca tgcggccgac ggatatgcaa gggcaaccgg
     5341 caaagtcggt gtctgtatgg caacatcagg tccaggagca acgaatcttg tgactggaat
     5401 cgcaacggcc tatatggatt ccgtgcctat ggtggcaatt actgctaatg tcatactgcc
     5461 gctgcttggg aaagatacct ttcaggaagt cgatattgca ggtgttacga tgccgatcac
     5521 aaagcacggg tatattgtca aggatgttaa agttttagcg gatacgatca gaaaggcgtt
     5581 tgtgatcgcg cgttccggaa gaccggggcc tgtccttgtt gatattacaa aggatgttac
     5641 ggcagacaaa acagaatata caccggtaaa accggttctg cttccgtcac cgtccgggta
     5701 tacgaaaaaa g
//
LOCUS       DAOSYE010000027         5623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9556_length_5623_cov_3.090876, whole genome shotgun sequence.
ACCESSION   DAOSYE010000027 DAOSYE010000000
VERSION     DAOSYE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_5623_cov_3.090876"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLU43_00915"
     CDS             complement(<1..612)
                     /locus_tag="PLU43_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26996.1"
                     /translation="MGKENSNKVIKSGIWYTISSISIRAIAIVTTPIYTSMLTTADYG
                     RANTFNSWIDLFNILTCLCIVYSIGRAKIDFGDRFDEYMSALQGLSSSFAFGVLILAL
                     IFREQLSAWIKYEVPLVIVLFVYLIISPSVEYMQQKCRYEYRYRENIVISIVSCIGTI
                     FFSVTLMLLFNEQRYFGKILGTLMTTFLMGILFYIRILKKGRIF"
     gene            complement(612..1682)
                     /locus_tag="PLU43_00920"
     CDS             complement(612..1682)
                     /locus_tag="PLU43_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOO26997.1"
                     /translation="MESMIINRLEKGKFVLIAEIGVNYYDIAEKEGITPLEAAKEMIR
                     AANDAGIHAVKFQTYQAGKLAAKASPSYWDLAEEPTTSQYELFKKFDHFGYEEYRELK
                     VYADSLGIEFLSTAFDFEAADYLDELMAVYKISSSDLSNLPFIKYQAQKNKPILLSVG
                     ASQLLEIEAAVDAIRMYSEQPIVLMHCVLEYPTPPEDANLLKIVSLTKQFPELYIGYS
                     DHTKPTKNCDVIKTAYNLGALCVEKHFTLDKTLKGNDHYHAMDQEDAKRILKGIQELD
                     LIRGKGDLVCLETEQTARKNARRSIVAKSAIAKGTAVTEEMLTFKRPGTGISPDRLSQ
                     LTGRIALEDIAEDTILMDWMFE"
     gene            complement(1682..3331)
                     /locus_tag="PLU43_00925"
     CDS             complement(1682..3331)
                     /locus_tag="PLU43_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO26998.1"
                     /translation="MNRECCGQEAGTVRSECMQVQDFKNKKILFVCRETYSMPLWFLA
                     RELKKDNDVAAFFIMSTECSYHKCYYNENSYYKFKEELPDVVLYDVKDICGQYTEGLK
                     RPGSPVDIGYLEKIEQEYSHFKNLNMQLMSSQENTRHYHFRYYWSLTNYDENMYWLEL
                     NYKKILSIFKEFEPDMILDFDNAELQRTIINEIAYKKGIPYITVEYSKFGYYKYPTFQ
                     NTIGIDAYVVRTYQENLKKSKEALAAGYAYVQEFRSKTTIMNKEFAGTVTSQYERDSL
                     LWILRVMHGKWNYFWNMDITNKNLKIKRTNKILYAPSVPYFFHYLQVLLLKRKFMGKN
                     RYFENPVPGEPYVYMPLHLIPESTVFVKASYYVDECNLIEQVSKSLPAGWKLYVKEHQ
                     AMLGERSVKFYKKVKELHNVRLVQVNYYHDPKPWIINAKGVITIVGTTAYEEALLGRK
                     SIIFGDVPFELIDGITRVRSFEDLPELIRDFGEIDNIHSCAAYLQTIMEVGFEIDLFY
                     LMAKAEKVLAGKEQEDDKFKEQLYRLMEFYKAGFARYQEEH"
     gene            complement(3285..4334)
                     /locus_tag="PLU43_00930"
     CDS             complement(3285..4334)
                     /locus_tag="PLU43_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO26999.1"
                     /translation="MPKVSIIIIIYRVERFLEQCLKSVTEQTYENLEIICVVGKGDSA
                     CEEICDRTAAGDPRVIVLKKEPQGTAVARNQGLDAASGEYIGFVDGDDYIAPDMIEIM
                     VNAAKKYQADISVVGKFYTYENCIDGTTEDKEYVYHTQQAFETILYQEGFFLHLWDKL
                     YKRELFRSIRFLPGQLVEDRQIACSILLQADRIVYHTASKYYFRVSEDSGSHIEKNLM
                     LSLQADYEICEKLLELYPGLQDAVDFFLVYENMSVIQNSILYGAFSKEHDQEYLSYVA
                     THAKKVKKDPRVPGSIKMKMTLCNISGTLFSIVTRYRRNKFLKNHRLYRTGIDWTATF
                     HEQGMLRSGSRNGKE"
     gene            complement(4341..5423)
                     /locus_tag="PLU43_00935"
     CDS             complement(4341..5423)
                     /locus_tag="PLU43_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOO27000.1"
                     /translation="MKIMCMYLPQYHTFEENDKWWGEGYTEWTAVRQAVPLYKGHMQP
                     RIPLDQRYYDLVKQGVETWQWQAFLAKQYGIYGFSIYQYWFLGKQLMGKPMELLLAHP
                     EIDIRYSICWANETWTRTWYGLADQILMKQDYGAEPDWRTHFEYLLPFFKDERYVKED
                     GKPVFQIYRSFDIPCLQEMLACFHKWAKEAGFPGIYVVSGKTAGLQEQRRQLIDAYYY
                     FEPGYTLKHDFGRFHQSCYNGSVLLRSLFNKVSGKKILERRIPGGWILDRIAGRHYEE
                     NEFPGLIPDWDNTPRRSYKGLVYKNTNPAAFEKVLRTLKEKVSGDRADYIFINAWNEW
                     GEGAMLEPDEVKGYGYLEAIRRVAEE"
     gene            complement(5429..>5623)
                     /locus_tag="PLU43_00940"
     CDS             complement(5429..>5623)
                     /locus_tag="PLU43_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27001.1"
                     /translation="LLIFIKSTGRLKDCYVELGIISVALLQSIGSNILSFQLAAPIFW
                     FAVGRCAFLIQTRTEPTRSQ"
BASE COUNT         1456 a         1449 c         1068 g         1650 t
ORIGIN      
        1 aaaaatgcgg ccttttttca aaatgcgaat atagaacaaa atacccatca aaaaggtcgt
       61 catcagtgtg ccgagtattt tcccgaaata ccgctgttca ttgaacagaa gcatcaatgt
      121 cacggagaaa aaaatcgtgc cgatacagga aacgatcgag atcacaatat tttcccggta
      181 tctgtattca tatctgcact tttgctgcat atactctacg gaaggggata tgatcagata
      241 aacgaacaga acgatcacaa gcggaacttc gtacttgatc catgccgaaa gctgttccct
      301 gaaaatgaga gcaaggatca gcacaccaaa agcaaacgaa ctcgagagtc cctgcagtgc
      361 ggacatatat tcgtcaaacc ggtccccgaa atcgattttc gcacgtccga tactgtatac
      421 gatacacaga caggtcagta tgttaaacag atcgatccat gagttaaacg tattggcacg
      481 tccgtaatcc gcagtcgtca gcatacttgt atagatcggt gtcgtaacga tcgcgatcgc
      541 gcggatggaa atactcgaaa tcgtatacca gattccggac tttatcactt tattactgtt
      601 ttcttttccc attattcaaa catccagtcc atcagaatcg tatcttccgc aatatcctcc
      661 aaagcgatcc tgcctgtcag ctgcgaaagc cgatccgggg aaatccccgt tcccggccgc
      721 ttgaaagtca gcatttcttc cgtaacggct gttcctttgg caatggcgga ttttgctacg
      781 atcgaacgtc ttgcattttt tcgtgcagtc tgttctgtct caaggcagac cagatcgccc
      841 ttgccgcgta tcagatccaa ttcttggatc ccttttaata tccgttttgc atcttcctga
      901 tccattgcat gataatgatc gttgcctttc agtgttttgt caagtgtaaa atgcttttcg
      961 acacagaggg cccccaaatt atatgctgtt ttgatcacat cacagttttt tgtcggtttt
     1021 gtatgatcgg aatatccgat atacagctcc ggaaactgct ttgtcaaaga tacgatcttc
     1081 agcagattgg catcctctgg cggtgtcgga tactccagta cgcagtgcat cagtacgatc
     1141 ggctgttcac tgtacatccg gatcgcatcg actgctgcct cgatctccaa aagctgtgaa
     1201 gcaccgaccg acagcaggat cggcttgttc ttttgggcct gatacttgat gaacggcagg
     1261 tttgacaggt ccgaagaaga aattttgtag actgccatca gttcatccaa ataatccgca
     1321 gcttcaaaat caaacgcagt ggaaaggaat tcaatgccaa gactgtcggc atataccttc
     1381 agttctctgt attcttcata tccgaaatga tcaaattttt tgaacaattc atattgggaa
     1441 gttgtcggtt cctccgccag gtcccaatat gaaggagacg cttttgccgc aagttttccc
     1501 gcctgatagg tctgaaattt cacggcatga ataccggcgt catttgccgc ccttatcatt
     1561 tcttttgccg cctctaacgg tgtgatgcct tctttttctg caatgtcata ataattaacg
     1621 cctatttcgg ctatcaggac aaattttccc ttttccagcc ggttgattat catactttcc
     1681 atcaatgctc ctcctgatat cttgcaaatc ccgctttata aaattccatt aaccggtaca
     1741 gctgctcctt aaatttatcg tcctcctgct ctttgccggc aagtaccttt tccgcttttg
     1801 ccatcagata aaacagatca atctcaaaac ccacctccat gatcgtctgc agatacgcag
     1861 cgcaggaatg aatgttgtcg atctccccga aatccctgat caattccggc agatcctcaa
     1921 agcttctgac ccttgtaata ccgtcgatca gttcaaacgg cacatctcca aagatgatcg
     1981 acttgcgtcc gagcagcgcc tcttcataag ccgttgtccc aacgatcgtt atgaccccct
     2041 tcgcgttgat gatccacggt ttcggatcat gataataatt cacctgtaca agcctgacat
     2101 tgtgcagctc tttcactttt ttgtagaact ttacgctcct ttcgccaagc atggcctgat
     2161 gctccttgac gtaaagcttc catccggcag gcagtgattt ggatacctgt tcgatcagat
     2221 tgcattcatc cacataataa gaggccttta cgaataccgt tgactccggg atcagatgca
     2281 gaggcatata tacatatggt tcccctggga ccggattttc aaaatacctg ttttttccca
     2341 taaattttct tttgagcagc aaaacctgca gatagtgaaa aaaatatgga acactcggcg
     2401 cgtacaaaat tttatttgtc cgtttgatct tcagattttt atttgtaatg tccatgttcc
     2461 agaaatagtt ccatttgccg tgcatcactc gcaggatcca gagcagactg tcccgttcgt
     2521 actgactcgt taccgttcct gcgaattcct tgttcatgat cgtcgtttta cttcggaatt
     2581 cctgtacata ggcatagccg gcagcaagcg cttcctttga ttttttcaaa ttttcctgat
     2641 aggtcctgac aacataggca tcgataccga tcgtattttg aaacgtcgga tatttatagt
     2701 acccaaactt cgaatattcc accgtgatat acgggattcc ctttttatag gcgatctcat
     2761 tgatgatcgt gcgctgcagc tccgcattgt caaagtccag gatcatatcc ggttcaaact
     2821 ctttgaaaat agagaggatc tttttatagt tcaattcaag ccagtacata ttttcatcat
     2881 aatttgtaag tgaccagtaa tacctgaaat gatagtgtct cgtattttcc tgcgaactca
     2941 tcagctgcat attcaggttt ttaaaatgag aatactcctg ctcgatcttt tccaaatagc
     3001 cgatgtcaac aggggaaccc ggacgcttca gtccttccgt atactgtccg caaatatcct
     3061 ttacatcata cagcactaca tcaggaagct cctccttgaa tttataatag ctgttctcat
     3121 tataatagca tttatggtag ctgcattctg tggacataat aaaaaaagcg gccacatcat
     3181 tgtctttttt caattccctg gcaagaaacc ataaaggcat ggaataggtt tccctgcata
     3241 caaaaagtat ttttttattt ttaaagtcct gcacctgcat gcattcactc cttaccgttc
     3301 cggcttcctg accgcagcat tccctgttca tgaaacgtcg ctgtccagtc aatgcctgtt
     3361 ctgtataaac gatgattttt taaaaattta tttctgcgat accgggtaac gatcgaaaac
     3421 aatgtgccgc ttatattgca caatgtcatt ttcattttga tactgcccgg aacgcgcgga
     3481 tccttcttta ctttttttgc atgtgtcgcc acataggaga gatactcctg atcgtgttcc
     3541 tttgaaaaag cgccgtatag aatactgttt tgaatcacac tcatattttc ataaacaagg
     3601 aaaaaatcga ctgcatcctg cagtcccgga tacaattcca gcagtttttc gcagatctca
     3661 taatccgcct gcagggaaag catcaaattt ttttctatat gagaaccgct gtcttcactg
     3721 accctgaaat aatatttgga agccgtatga tagacgatcc tgtctgcctg taaaaggatg
     3781 ctgcaggcaa tctgcctgtc ctccacgagc tgtcccggta aaaaacggat cgaccggaac
     3841 agttcccttt tataaagctt gtcccataaa tgcaggaaaa agccttcctg atacaaaatc
     3901 gtctcaaagg cctgctgtgt atgataaacg tattctttgt cttccgttgt tccgtctata
     3961 cagttttcat acgtatagaa ctttccgacc acggaaatat cggcctggta cttttttgca
     4021 gcattcacca tgatctcgat catatccggc gcaatgtagt catcgccgtc cacaaatccg
     4081 atatactctc cggatgccgc atcaagcccc tgatttctcg caacagccgt accctgcggc
     4141 tccttcttca gtacgataac acgcggatcc ccggcagctg ttctgtcaca gatctcctca
     4201 caggcactgt caccctttcc gactacgcaa atgatttcca gattctcata tgtctgttcc
     4261 gtcacgcttt tcagacactg ttctaaaaaa cgctctactc tataaataat aatgataata
     4321 ctgacctttg gcattttgct ctattcctcc gccacgcgcc tgattgcttc cagatacccg
     4381 taccctttta cttcatccgg ttcaagcatt gcgccttcac cccattcatt ccatgcattg
     4441 ataaaaatat agtccgctct gtccccggat accttttctt ttaatgtccg aagaaccttt
     4501 tcgaatgcgg ccggatttgt atttttataa acaagtcctt tataactcct gcgcggcgta
     4561 ttatcccagt caggaatcaa tcctggaaat tcattctctt catagtgtct cccggcaatc
     4621 cggtcaagta tccagccgcc cgggatcctg cgctccagta tttttttccc gctcacttta
     4681 ttgaacaggg acctaagcag cacggaacca ttataacagc tttgatgaaa tctgccaaaa
     4741 tcatgtttca gcgtatagcc gggttcaaaa taataatagg catcgatcag ctgccgtctt
     4801 tgttcctgca aaccggcagt ctttccggaa acaacataaa tgcctggaaa tccggcctcc
     4861 ttcgcccatt tgtgaaagca tgccagcatt tcctgcaggc aggggatatc aaagctccga
     4921 tagatctgaa ataccggttt tccgtcttct ttcacatacc gttcatcttt aaagaacggc
     4981 agcagatact caaaatgcgt acgccagtcc ggctccgccc cgtaatcctg tttcatcaat
     5041 atctgatcgg ccagcccata ccaggttctt gtccatgtct cgtttgccca gcagatacta
     5101 tagcggatat cgatttccgg atgcgcaagc aggagttcca tcggctttcc catcagctgc
     5161 tttcccaaaa accaatactg ataaatactg aacccatata tcccgtactg ctttgcaaga
     5221 aaagcctgcc actgccacgt ctccaccccc tgctttacaa ggtcataata cctctggtcc
     5281 agcggaatac gcggctgcat atgtcctttg tacaggggaa cagcctgcct gaccgcagtc
     5341 cattcggtat atccttctcc ccaccactta tcgttttcct caaacgtatg gtattgagga
     5401 aggtacatgc acataatttt cataatccct actgactcct tgttggttct gtccttgtct
     5461 gtatcaaaaa agcgcagcgt ccgacggcaa accagaagat cggagccgcc agctgaaatg
     5521 acaaaatgtt ggaaccgatc gactgcagca gggccacaga aatgattcca agttccacgt
     5581 agcagtcttt cagccttccg gtacttttta taaagatcag cag
//
LOCUS       DAOSYE010000028         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9698_length_5568_cov_3.106838, whole genome shotgun sequence.
ACCESSION   DAOSYE010000028 DAOSYE010000000
VERSION     DAOSYE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9698_length_5568_cov_3.106838"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLU43_00945"
     CDS             complement(<1..275)
                     /locus_tag="PLU43_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO27002.1"
                     /translation="MLNYLFAVLFPGKPKEKKKGNLKNHKQIFSFTNNSVFATASADG
                     VPNIVPIHSKHLVPGNKVLISDQFMNKTKHNILQNPYGTLLIRDSGQL"
     gene            complement(345..800)
                     /locus_tag="PLU43_00950"
     CDS             complement(345..800)
                     /locus_tag="PLU43_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO27003.1"
                     /translation="MKNSKKIISLYFRSMKRFEELERVPQNFGTEHLLYSSEIHTIQA
                     IGDHPNINLTSLADSLGISKSGASKFIGKLLQKGLITKNKQINNDKEVVFNLTKTGYI
                     AYQKHEEFSKECFKPVYELLSGFDETQTAFLETFLKDLNTTIDQIKILQ"
     gene            complement(944..1261)
                     /locus_tag="PLU43_00955"
     CDS             complement(944..1261)
                     /locus_tag="PLU43_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO27004.1"
                     /translation="MILAEKILALRKSNSWSQEELAEKMNVSRQSISKWESAAAIPDI
                     NRIPELAKLFGVTTDYLLKDDIETTAYSDEYEEDGRIRVSLQESSEFIKSSTGYGKQH
                     AIY"
     gene            complement(1564..2538)
                     /locus_tag="PLU43_00960"
     CDS             complement(1564..2538)
                     /locus_tag="PLU43_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27005.1"
                     /translation="MKQIEELYNPVFKKCGFILKPQTKTEDHSICYELRPDKGQGSYR
                     IYYYQDMFEISFKNFFFYEDYFMECPEVEFLSVDYYSSVSGEEFHPYYQLSPDSLRVH
                     IGEKNKLFQAIYHKNVPIRSVGISMMPNFYSQYLRKKFSYDDFIPSEAFRQFQSGTDF
                     PQLAALLKQIEHYRGTGLAAKMFYEGKVLEAIALIIEKAKLIQKNRTKISLTETDSEG
                     LMNVVTYIDNHYAFQIPLERLCRISFMGSTKLKAAFKSLMGCTISEYILQKKIGHAQH
                     LLIGTDLTIAEIAKAVGYKRPDSFAKQFQKATGLLPREYRGLTTGPII"
     gene            2733..3446
                     /locus_tag="PLU43_00965"
     CDS             2733..3446
                     /locus_tag="PLU43_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO27006.1"
                     /translation="MFKVSKPDARILILQIILASYLSFSFNNLIALFLLFAIIDILVL
                     VWRGWRHCVRFLCIYAVFIMITGCLTFLNLSLLSTIFQLLSLLLIRIYPIFLLAGILL
                     AVTPMNELLTALEKAHVPKTLLIPLMVIYRYIPTLIREIRYVRESLKMRNSDISLSVF
                     LQHPAKYAEDFLVPLLYRSEKIAEELAAAAICKGLSVKRSRSCCTDVKLGPVDICYAA
                     GMTAVAAALICVNAFAPVI"
     gene            3474..3773
                     /locus_tag="PLU43_00970"
     CDS             3474..3773
                     /locus_tag="PLU43_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27007.1"
                     /translation="MVKIPDRSEIEFEKFSFTYEGSNAPALSDVSLKIGKGEFLVLTG
                     ESGCGKTTITRCINGLLPDFFEGMIKGHCSVRGMNIPFGRSGILPLHGFAAASKH"
     gene            3713..4804
                     /locus_tag="PLU43_00975"
                     /pseudo
     CDS             3713..4804
                     /locus_tag="PLU43_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4797..>5568
                     /locus_tag="PLU43_00980"
     CDS             4797..>5568
                     /locus_tag="PLU43_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27008.1"
                     /translation="MFKTIKRIIAWIVPYKKRLQLGMLCSFFAALFTAIPVMTSAWAL
                     SQAISDMRGEMPFRTEVIWIAFLAIAVSTFLRYLFSYGRARLQDSIGTEMASSERIRI
                     GDMLGRVSLGYFEKNSTGEILADLTGEFTQLETNGMKMIDIFVNGYIKFAALVLCLAV
                     FSPQAALVAVCSALLSIFALQGLNRQSRKQSPRNRQTNEALAETVIEYVRGLPVVKSF
                     GQEGAAIRALHNACRESREVNIVIAKAYQKWNVLNLFVL"
BASE COUNT         1488 a         1137 c         1282 g         1661 t
ORIGIN      
        1 agttgtccgg agtcccggat caacagtgtt ccataaggat tctgaagaat attgtgtttg
       61 gttttgttca taaactgatc actgatcaat actttatttc ccggcacaag atgtttagag
      121 tggatcggaa caatgttcgg cacgccgtct gcagaggcag ttgcaaatac ggaattattt
      181 gtgaagctga aaatctgttt gtgatttttg agatttcctt tctttttttc ttttggtttc
      241 ccaggaaata aaacagcaaa taagtaattt agcatcaaga gactcctttc tattaaccag
      301 gtgaataaaa tgaagatttt tttgccggct gtaaagccgg cgaattactg taaaattttg
      361 atttgatcga tcgtcgtatt cagatccttt aaaaaagttt ctaaaaaagc tgtctgagtt
      421 tcatcaaaac ctgacaacag ttcataaaca ggcttaaaac actcttttga aaactcctca
      481 tgtttctggt acgcgatata tcctgttttg gtcagattaa aaacaacttc tttatcattg
      541 ttgatctgtt tgttttttgt gatcagacct ttctgcagca gtttaccaat aaacttggaa
      601 gcgccgcttt tggatattcc caggctgtct gccaggctgg tcagattgat atttggatga
      661 tctccgatcg cctgaatcgt atggatttca gagctgtaga gcaaatgttc ggttccgaaa
      721 ttctggggca cgcgttctaa ttcttcaaag cgtttcatgg aacgaaaata cagactgata
      781 attttttttg aatttttcat agtatcacca taacaatggt acaccaggtt aacaataatg
      841 tcaagtcttt tcgtttcaaa gcatatttct gcaacggtgc gaacactttt gcgaacaaac
      901 aggatataat aatcgcagac gagtatacga ttgtcgtttc cggtcaatat attgcatgtt
      961 gttttccgta accggtactg ctttttatga actcgcttga ttcctgtaac gaaacacgaa
     1021 tacgtccatc ttcttcatat tcgtcggaat atgccgtcgt ttcaatatcg tcctttaaaa
     1081 ggtagtctgt agttacacca aaaagttttg ccaattccgg tattctgttg atgtcaggaa
     1141 tagcggcagc actttcccat tttgagatcg actgtctcga cacattcatt ttttcagcca
     1201 gttcttcctg agaccagcta ttgctttttc tcaatgctaa aattttttct gctaaaatca
     1261 tattgctccc tcacttttga attttatatt cgtatcattg caggaaaagc atactgtatt
     1321 ttggggttac atgctatcaa gacggcttta aaattcagca actgacagtt gcaatttaat
     1381 aataacaggt atctttagta agtccatcta attctataat catagatgga tacatttgtt
     1441 acaaaaattc tattatcaaa gaaataccgg tatctgcgga atacccattg ttcttctgga
     1501 tgagccgacg agcggactgg atggtggaca cctgtgacag agttctttgc ctgaacaagg
     1561 gccctatatg attgggccgg ttgtcaggcc tctgtactcc cgaggtaata gacccgtagc
     1621 tttttgaaat tgttttgcga aactgtcggg ccttttatag ccaacggctt ttgcaatttc
     1681 agcaatggtt aagtccgtgc cgatcagcag gtgctgcgcg tgaccgattt ttttttgcag
     1741 aatatattct gatattgtgc agcccattaa cgacttgaaa gctgccttga gctttgtact
     1801 tcccataaaa gaaatgcggc acaggcgttc caatgggatc tgaaaagcat aatggttatc
     1861 aatatatgta actacgttca taagaccttc tgaatcagtt tcagtcagag aaattttagt
     1921 tctgtttttc tggatcagtt ttgccttttc aataatcaat gcaatcgctt caagaacttt
     1981 cccctcataa aacatctttg cggcaaggcc cgtgcctctg taatgctcaa tctgtttcag
     2041 gagcgctgcc agctgtggaa aatcagttcc ggattggaat tgtctgaagg cttcactggg
     2101 aataaaatca tcataggaaa attttttccg taaatattgg gaatagaaat ttggcatcat
     2161 ggaaattcca acagatcgga tcgggacgtt tttatgatag atcgcctgaa acagcttatt
     2221 cttttccccg atatgcacac ggagactgtc gggcgaaagc tgataataag gatggaattc
     2281 ttcccctgaa acggaagaat aataatccac gctcaggaac tcgacttcag ggcattccat
     2341 aaaataatct tcatagaaga aaaaattttt aaaggaaatc tcaaacatat cctggtagta
     2401 atagatccgg taactgccct gtcctttgtc aggacgcagt tcataacaga tggaatgatc
     2461 ttctgttttt gtctgcggct ttagtataaa tccgcatttt ttaaaaacgg gattgtagag
     2521 ttcttcaatt tgcttcatgc ggtttgctcc atcatacgtc tgccggacgc aatcatacgt
     2581 ttgggggacg cagcatctta aggaatgttg aaaaaaatca atagagaagt atacttaagc
     2641 ttgttggtta gacaatacta accaatggca ttataacatt cattttatat ttcgacaaga
     2701 aaaacaagga taggagaagg tgcgtggagt tagtgtttaa agtttcaaag cctgatgcaa
     2761 gaattttaat tctgcagatc atccttgcat cttatctgag ttttagcttt aacaatctga
     2821 ttgcgctgtt tttgcttttt gcgataattg atattctggt gttagtgtgg cgcggatggc
     2881 ggcattgtgt acggttttta tgtatatacg cagttttcat aatgataaca gggtgtttga
     2941 cgtttttaaa cctgtcgctg ctttccacaa tttttcagtt gttatctttg ctgctgattc
     3001 gcatatatcc gatatttctg cttgcaggga tacttctggc cgtgactccg atgaatgaac
     3061 tccttacggc gctggaaaaa gcacatgttc ctaaaacgct gctgattccg cttatggtca
     3121 tttaccggta tatcccaaca cttatccgtg agatcaggta tgtccgcgaa agtcttaaaa
     3181 tgagaaattc cgacatttca ttgtcagtat ttctgcagca tcctgcaaaa tacgcggaag
     3241 actttcttgt tccccttttg tatcgcagcg agaaaatagc cgaagagctt gcggcggcag
     3301 caatatgtaa aggacttagt gtgaagcgca gtcgttcctg ctgtacagat gttaagctcg
     3361 ggccggtcga tatctgctat gcggccggta tgacagccgt ggcagcggca ctgatctgtg
     3421 tgaatgcttt cgcccccgtc atttaaacag gaggaaaggg agtacgtcaa aatatggtaa
     3481 agatacctga tcgcagtgaa atcgaatttg agaagtttag ttttacatat gaagggagta
     3541 atgccccagc gctgtcagat gtcagcctga aaatcggcaa aggggagttt ctggtgctga
     3601 caggggaaag cggctgcgga aagacgacga ttacccgctg tataaacgga ttgctccccg
     3661 acttttttga aggaatgata aaggggcatt gttctgtgcg cgggatgaat attccattcg
     3721 ggcgttcagg gattttgccc ttgcatggtt tcgcagcagc ttcaaaacac tgacgcttat
     3781 ttcttcggtc gtacctacta cgctgcttgc aacggttcct gtcggcctgc tgctctaccg
     3841 ggacggcagc ctgagcgcac cggaactgac tatgtgctgt attctggcgc tcggtcttgt
     3901 tccgccgctt ctcaagctta caacatacct caatctcggg aaaatgattc aatatgcgat
     3961 cttggcggcg gatgagcttt tagatctgcc ggagcttccg gaaaaaaaca gcggcaagct
     4021 ccctctggaa tactctattt gtttaaaata cgtatcattt tcttatttgg gaggaaacga
     4081 agtcttacat aaaatcaatc tggatattcc acaaggcagc gtcaccgcac ttgtcggacc
     4141 atccggcggc ggaaaatcca caatagcaaa attgatcact cgtttttggg atgtggatga
     4201 aggggcaatc tgtatcggag ggaccgatat cagagagata cctcttgcaa agctgtccaa
     4261 aatggtcagc tttgtttcac aggataattt tctgtttaac tgttccctta aggaaaatat
     4321 ccggcttgga gattctgccg cctcagatga agaggtgttt aaggctgcga gggcggccag
     4381 gtgcgaagag tttattacga ggctgcctgg gagctgggac actccggcag gtgaggcggg
     4441 gaaatgcctt tccggcggcg aaaaacagcg tgtttccatt gcccgggcga tcctgaaaaa
     4501 tgcgccgatc gtcattctgg acgaggccac ggcctttgcc gatcccgaga acgagaataa
     4561 aatacagcag tccattatgg aattagccaa aggtaaaacc ttattggtaa ttgcacaccg
     4621 cctttcaacg atcaggaatg cagagcagat tatcgttatg gaaaacggcg gcgttgccgc
     4681 ttctggaaca catgagcagc ttttaaagaa ttgcggcctg tatggccgtc tctggaatgc
     4741 ccatatgggt gcgcgccggt gggcagtgtc ttcagggaaa caggaggaag agaaacatgt
     4801 ttaaaactat aaagagaata attgcatgga tcgtacctta taaaaaacgc ttacagcttg
     4861 gaatgctgtg ttcttttttt gcagccttat ttacagcaat ccctgtaatg acttctgcgt
     4921 gggcgctcag ccaggcaata tcggatatgc ggggagaaat gcctttcagg acagaggtca
     4981 tctggattgc atttttggca attgcagtat ccacattcct gcgttatctg ttttcctatg
     5041 gccgcgcgag actgcaggac agcattggta cagagatggc ctccagtgaa agaattcgta
     5101 ttggagacat gctggggcgt gtatctctgg gctattttga aaaaaacagt acgggtgaga
     5161 ttcttgccga tcttaccgga gaatttacac agcttgaaac caacggaatg aaaatgatcg
     5221 acatatttgt caatgggtat atcaaatttg cggcactggt tctctgtctt gcggtattca
     5281 gcccgcaggc agccctggtc gctgtgtgca gcgcgcttct gtcaattttt gcgctgcagg
     5341 gcttaaaccg gcagagcaga aagcagtccc cgcgtaaccg gcaaacgaat gaagccctgg
     5401 cggaaactgt gatcgaatat gtccgtggtc tgccggtagt caaatctttc ggacaggaag
     5461 gagcagctat ccgggcactg cacaatgcgt gcagggaaag ccgtgaggtc aatattgtga
     5521 ttgcaaaagc ctatcagaaa tggaatgtgc tgaatctttt tgttttaa
//
LOCUS       DAOSYE010000029         5544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9761_length_5544_cov_3.412461, whole genome shotgun sequence.
ACCESSION   DAOSYE010000029 DAOSYE010000000
VERSION     DAOSYE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9761_length_5544_cov_3.412461"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..661
                     /locus_tag="PLU43_00985"
     CDS             179..661
                     /locus_tag="PLU43_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO27009.1"
                     /translation="MDISTIILYTVAIVLLVVSIIKDREKTKKAVRKGWMAFVNILPI
                     LIPLFLIVGVALSVLTPQIIRQVLGEESGIFGVFTGMIVGSAAFMPPFVTYPLGAELL
                     KSGAGYPQVAAFITTAMAVGLVYWAAETKYFGTKAVALRNGLAFVASGTVAFVVWGVL
                     "
     gene            complement(1117..2064)
                     /locus_tag="PLU43_00990"
     CDS             complement(1117..2064)
                     /locus_tag="PLU43_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27010.1"
                     /translation="MKLLIVNDAVLEAETMKRDIAWNNYHIDEVYLAFSAEEGRRIVL
                     EKQVDILLCDIEMPGENGISFIRWIKKNQYDIDCILLTCHADFSYAREAVSLGCQDYI
                     LLPAKYDEIGETVHTVVKRRRLKLENLQLQELGQSWLTAKAESIVSYPAKSARDIVDE
                     CQRYILDNISNDNLNISDISSKLYLSPTYLNRLFKKEKGISISQWILKERMELAAFLM
                     KDTSCSAFTIAEQVGYLNYPYFSTVFKKFFGQSPCSILKTYLRSPIYKPFDSRPLCAF
                     RKCIDLVFFFGGKQLFNKQCPDQIYGKQGDIRYDRIRNP"
     gene            complement(2091..3170)
                     /locus_tag="PLU43_00995"
     CDS             complement(2091..3170)
                     /locus_tag="PLU43_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO27011.1"
                     /translation="MINLQNDLTDIKNSITYKNTTLFFDSSPSGKKSGYMAVSSASSK
                     TDLYINLLIPTSAFTKNVAPMKKVIIGAFAVYLFIIPLLYFLFKREVVHPLHTLNDAH
                     QQLVLGNEDYHIEVPALSSDFHYAYRSFNTMSDTLKRLRIENLNKELFAKQLHLDNLK
                     LQIRPHFLLNTLNLLYTLIQDHQDVPAQELTLYLSKYFRYMFRNEEKLAMFDSELSLI
                     KEYLKISAIHYPNEFQVSYQIDPVISYMRLPPLLLHNFFENIIQHSLIKGHVIHVVLF
                     AEYENETVTIQISDDGRGMSPEEVQRINDKEFLSTNSGQHIGIRNSINRLKYYYGDDA
                     GLSVESESGRGTTFTLTIPYNLEDV"
     gene            complement(3197..3730)
                     /locus_tag="PLU43_01000"
     CDS             complement(3197..3730)
                     /locus_tag="PLU43_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27012.1"
                     /translation="MKIIKKKHTEKNHYHSLVVKTVISLLLLILPLNTLSLMTANSMI
                     RDAQEILQNSAVLPLNSHMNIIDNKITNSNYFLYNLPSSNADCIAMLAQENDWTYTYH
                     RTLVFQTVYDSLSMTNSADHLFFYMKEKNDILNIANNSSEKYQTNTCMAYWLLKNCLT
                     PNGICLIMKGSATCSVR"
     gene            3915..5522
                     /locus_tag="PLU43_01005"
     CDS             3915..5522
                     /locus_tag="PLU43_01005"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27013.1"
                     /translation="MKKKILTVILALTAALGLMACGAADKEADVQTAESTESTAEEGQ
                     ETAEQSDDATYHVEMQILTWGTVPTGLEAVEQAINEVTEPEIGVTVTLNPVAAWDLAS
                     ESSMAITSGEKMDLICILPYGSGLDSVANYTSKNMLTPLDDLYAEYGQDIAAHVDADV
                     LSLGYQGGNLYAVPVNGILGVTPCFVARTDILDELGISIDEDKVYSLDELEEMFDAYK
                     ETYGNGYYCVAAFGGMDMYNMINRIDNLGTDDSDGVLIGAGMDGDTTVVDKFETKEYE
                     DFTYKMYEWYQKGFFSPDVTTITDGTTLILAQGNYFGTFGGISPGIGFETMKAGCGYD
                     LTIIKTAESYTTSGIASMCLWGIPVTSENPEKTMQFLNLLYQDREIGKDIDTMLSIGL
                     EGSDYEVVEEVEGSKAIVKYPEGLDLNTVSWAFTAPIYGNQLTIPNYEPLTTDIYNEY
                     ETYNENVLAEGNKSCVFGYVYDSSNMSTQKAAVASVISQYQPILSCGSVDPAEALPEF
                     IQALKDAGIDELVADNQAQLDAWIADR"
BASE COUNT         1603 a         1024 c         1361 g         1556 t
ORIGIN      
        1 aacagaccct tatagggtca gagcaaacca cccgttggac gggtggttat gatttgtcgt
       61 agtatgcctt catatcacaa aaggctccct gttgtttatg acaaagtcac agaaaaataa
      121 aaaataacaa aatataataa ctaactaaga aaaataatca gaaaaaggag gagcatttat
      181 ggatatttca acgattattt tatatacggt tgcaattgtt ttgttagttg tatcgattat
      241 aaaagacagg gaaaagacaa aaaaagcagt cagaaaagga tggatggcat ttgtaaatat
      301 acttccgata ttgataccgc tgtttttgat cgtcggtgtt gcgctcagtg ttttgacacc
      361 gcagataatc agacaggtac tgggggagga gtccggcata tttggtgtgt ttacaggaat
      421 gattgtcgga tctgcggctt ttatgccgcc gtttgttacg tatccgcttg gtgcggagct
      481 tttgaaaagc ggtgccggct atccgcaggt ggcggcattt ataacaacag caatggctgt
      541 cgggctagta tactgggcag ctgagacaaa atatttcggg acgaaagccg ttgcactgag
      601 aaacggactg gcctttgttg cttccgggac tgtagctttc gttgtatggg gggtattgtg
      661 atgaaaaaag aaaaagaaat tctgctttct gtttttaaaa gcaaaatgtt ttatatttcg
      721 atcgccatgc tggcggtatt gtttattctg gatgccctgc atatcagact tggggcatct
      781 gatacggaaa cctcattagg agttacagct gccggttccg cgctgtctca gttcatttcc
      841 atgttaaaga tcgttccgcc gatatttctg atgatcgggc tgctggatat ctgggtgcca
      901 agagaaacga tgatccgtct gatgggagaa ggctccggca tgcgcggtat cctgatcgcc
      961 ctgtttattg gaacgatgtc ggccggtccg cttgtagcgg cttttccggt tgcacaggtc
     1021 atgctgaaaa agggcgcccg gtattccaat gtcttattct tcctgactgt ctgggccagt
     1081 gcaaaactgc cgatcctgtt ttttcaggca acggaattag gggttgcgta tacggtcata
     1141 acgaatatca ccctgcttgc cgtatatctg atcgggacac tgtttattga aaagctgctt
     1201 tccgccgaag aaaaagacaa gatctatgca cttgcggaaa gcgcataaag gacgagagtc
     1261 aaagggttta tatatcgggc tcctgagata tgtttttaat atactgcaag gagactgtcc
     1321 gaaaaatttt ttaaataccg tggaaaaata cggataattc agatatccta cctgttccgc
     1381 tatggtgaac gcggaacagg aggtgtcttt cattaaaaat gcggccagct ccattcgttc
     1441 ttttaatatc cactggctga tgctgatgcc cttttctttt ttaaagagcc ggttcaggta
     1501 agtaggactt aaatataatt ttgaagaaat atcggaaata tttaaattat cattactgat
     1561 attatctaaa atataccgct ggcattcgtc aacaatatcc ctggcagatt tggcagggta
     1621 agagacgatg gattcagctt ttgcagtcag ccagctttgg ccaagttcct gaagctgcag
     1681 gttttcaagc ttcaggcggc gtctttttac aacggtgtgc actgtttcac cgatctcatc
     1741 atattttgcg ggaagcagga tataatcctg acagccaagg gagacggctt ctcttgcata
     1801 ggaaaaatca gcatgacaag tcaataagat acaatcaatg tcatattggt tctttttgat
     1861 ccaccggata aaggaaattc cgttttctcc gggcatttca atatcacaaa gaagaatatc
     1921 cacttgtttt tccaaaacga ttctgcgtcc ttcttccgcg ctgaaggcaa gatacacttc
     1981 atcaatatga tagttattcc aggcaatatc ccgtttcatc gtttccgctt ccaaaacagc
     2041 atcatttacg atcagtaatt tcataacaac ctcttttctt tataagcaga ctaaacatct
     2101 tccagattat aaggaatcgt cagtgtaaaa gtagtacccc gcccggattc ggattccacg
     2161 gacagtccgg catcgtcacc gtaataatat ttcagtcggt ttatggaatt gcggattccg
     2221 atatgctgtc cggaatttgt tgaaagaaat tctttatcgt ttatccgctg cacttcttca
     2281 ggggacatgc ctctgccgtc atcggagatc tgaatagtga ctgtttcgtt ttcatattcg
     2341 gcaaagagga caacatggat aacgtgtcct ttgatcaggg agtgctgtat gatattttca
     2401 aaaaagttat gcagaagaag gggcggcagc cgcatatagc tgattacagg gtcgatctga
     2461 taagatacct gaaattcatt tggataatga atggccgata tttttaaata ttctttgatc
     2521 agagataatt cactgtcaaa catggcaagt ttttcttcat ttcggaacat ataacgaaaa
     2581 tatttggata aatataaagt cagttcctgc gcgggtacgt cctgatgatc ctgtatcagc
     2641 gtatataaca ggttcagtgt attcagcaga aagtgcggac ggatctgcag cttcagattg
     2701 tccagatgca gctgctttgc aaataattct ttgttcagat tttcgatccg cagtcttttt
     2761 aaagtatcgg acatcgtgtt aaatgaccgg taggcataat gaaagtctga tgaaagggca
     2821 ggaacttcaa tatgataatc ctcattgccg agaaccagct gctgatgcgc gtcgttcaag
     2881 gtatgcaaag gatgtacgac ttcccgtttg aacaggaagt acagcagcgg aatgataaaa
     2941 agatatacgg caaatgctcc tatgatcact tttttcatgg gggctacatt ttttgtgaac
     3001 gcggaggttg ggatcagtaa attaatatac agatccgttt tggaagaagc ggaagagact
     3061 gccatatatc cgcttttttt gccggaaggg gaagaatcaa aaaaaagtgt ggtattttta
     3121 taagtaatgg aatttttaat atccgtcaga tcgttctgca ggttgatcaa tactccgtag
     3181 cagtaggaag gctggtctac cgtacggaac aggtagctga ccccttcata atcaagcaga
     3241 tgccatttgg agtcaagcag ttctttagaa gccagtaagc catacaggta ttcgtttggt
     3301 atttttccga ggaattattt gctatattta aaatatcatt tttttctttc atgtaaaaaa
     3361 ataaatgatc tgcggaattt gtcatggaaa ggctgtcata aaccgtttga aaaacaagag
     3421 tcctgtgata cgtataggtc cagtcgtttt cctgcgcaag catggcgatg cagtcggcat
     3481 tactgcttgg caggttatac agaaagtaat ttgaatttgt tattttattg tctatgatgt
     3541 tcatatgtga attgagcgga agcacagcag aattttgcag tatttcctgg gcatccctga
     3601 tcatggaatt ggccgtcatc agagacaggg tattgagcgg aagaatcagc agaagcagag
     3661 aaatgacagt ttttacaact aaggaatgat aatgattttt ctctgtgtgt ttctttttta
     3721 taatcttcat aggcagcggc tccgtaaatg ataatagcgt atttgttatg tttactatta
     3781 taaaattcgg cattaaaaat gtaaacagac aaggagaaaa aaggtagaat tttgaaagaa
     3841 tggtttcata tattgaatgt tcaaaccggg gaaggaactt ttataataaa agtatcagaa
     3901 aaggaggttt taaaatgaaa aagaaaattt tgacagtgat actggcactg acagcggctt
     3961 tgggtctgat ggcctgcggg gcagcagata aagaagctga tgtacagact gcggaaagta
     4021 cggagagtac cgcagaggaa gggcaggaaa cagcagaaca gtcagacgat gcgacctatc
     4081 atgtggagat gcagatactg acgtggggaa cagttccaac aggactggag gcagtggagc
     4141 aggcgattaa tgaagtgacg gagcctgaaa tcggcgttac cgtcacactg aatccggttg
     4201 cggcgtggga tctggcatct gaaagcagca tggcgatcac atccggtgaa aaaatggatc
     4261 tgatctgtat tttgccgtat ggttccggac tggattccgt tgcgaactac acaagcaaaa
     4321 atatgctgac accattggat gatctgtatg cggaatatgg acaggatatt gcggctcatg
     4381 tagatgcgga tgtattgtca ttaggatatc agggaggaaa tctgtacgcg gtaccagtaa
     4441 acggaattct tggagtgacg ccctgctttg tagcaaggac ggatattctg gacgaacttg
     4501 gcatcagcat tgatgaagat aaggtctatt ctctggatga acttgaggaa atgtttgatg
     4561 cttataaaga aacgtatgga aacgggtatt attgcgtggc agcgtttggc ggcatggata
     4621 tgtacaatat gatcaaccgg atcgacaatt tgggaacaga tgattccgac ggcgtactga
     4681 tcggtgcagg catggacgga gacacgactg ttgtagacaa atttgaaaca aaagaatatg
     4741 aggattttac ctacaaaatg tatgaatggt atcagaaagg atttttctcg ccggatgtaa
     4801 cgacgatcac ggacggaaca acactgatcc ttgcacaggg aaattatttt ggtacgttcg
     4861 gcggtatttc tcctggaatc ggatttgaaa caatgaaagc cggctgcgga tatgatctga
     4921 cgatcattaa gactgcggag tcatatacga caagcggaat tgcttccatg tgtctgtggg
     4981 ggatcccggt tacaagcgag aatcctgaaa agacaatgca gtttttaaat ctgctgtatc
     5041 aggacagaga gattgggaag gacattgata cgatgctgtc cataggactg gaaggttctg
     5101 attatgaagt cgtagaagag gtcgaaggca gcaaggctat tgtaaaatat ccggaagggc
     5161 tggacctgaa cactgtttca tgggcattta ccgcgccgat ctacggaaat cagctgacga
     5221 ttccaaatta tgagccgctg acaacggata tttataatga atatgaaacc tataatgaaa
     5281 atgtgctggc agaaggcaac aaatcatgcg tattcggcta tgtttacgat tcttccaata
     5341 tgtctacgca gaaagcggca gtggccagcg tcatcagtca gtaccagccg atcctgtcat
     5401 gcgggtccgt ggaccctgcg gaagcattgc cggaattcat ccaggcactg aaggatgcgg
     5461 gcattgatga actggttgcc gataatcagg cacagctgga cgcatggatc gcagacaggt
     5521 gatcgataac gggtaactgc cgtt
//
LOCUS       DAOSYE010000030         5542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9767_length_5542_cov_2.985238, whole genome shotgun sequence.
ACCESSION   DAOSYE010000030 DAOSYE010000000
VERSION     DAOSYE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_5542_cov_2.985238"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLU43_01010"
     CDS             <1..195
                     /locus_tag="PLU43_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27014.1"
                     /translation="QAAMDRLTKGRTSFVIAHRLSTIKNADLILVMKDGDIVESGSHK
                     ELLAKGGFYAELYNSQFEAA"
     gene            419..1843
                     /locus_tag="PLU43_01015"
     CDS             419..1843
                     /locus_tag="PLU43_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27015.1"
                     /translation="MIKNYKKIIAMLLTTAMTVSLCACGSSSAEQTTSDTTTDAAADT
                     TEAAETETADTETAPAAEEAGTDGLTGSFSIFHFHEEDGAGTSKAFWAAANAFSEENP
                     NVTIDYQFTDSDNYQEKLTTLMAGDELPDVFLTKGDMLGTLADAGMIQPLDQYIQADA
                     EWAAAYSDGVFSDCTYDGTEWGAPFQMQANCIGVYNEEIFEECGVDGWPETWDEMLDA
                     CEKIKAKGYTPIAMGNKDQWLAESAVFNTYAYKYVDGDWFDSLKNNTGAKFTDDKFVE
                     ALTGFQEIADKGYLNSDVNSINQDEMYALYYNKQAAMFFNGAWSIGTLTENAPEDVLA
                     NTHLGLMPAVSGQSGTKYDTAAGAGWNYVIRADLEGDDLTAALAFIKQVTTGDYADNA
                     LRNGFFSAAKQSGNVSESELPTLFAEYSALESTMNFLPIFDCQLPAEIGSGVLYADTQ
                     QLIADTITPEDMAADCQSTMEANY"
     gene            1927..2817
                     /locus_tag="PLU43_01020"
     CDS             1927..2817
                     /locus_tag="PLU43_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27016.1"
                     /translation="MNVRQVRSKRQYALIYTLVLIPFLYFLYILIIPLGTSAYYSLTK
                     WKGVGDPKFIGLKNYITLVHDSNFWLVTKNTVILSLLCVLGQVGIALIIAFLMTIRRL
                     KWKSFHRAVIFFPVVMAPIVVGYVWRFIYNSNYGLLNMFLNAIGKSSWIKNWLDDHDI
                     VLRMVSIPVIWQYIGLYMILLMSGISAIPTEVFECAEIDGATGLKKSLYITLPLIWDT
                     LKICIILCASGTMKIYDHLVALTNGGPGKASSSLALYCYDYTFKFGNFGMGAAIAVVI
                     LLLAVGISLLVQLLMGRKEA"
     gene            2819..3655
                     /locus_tag="PLU43_01025"
     CDS             2819..3655
                     /locus_tag="PLU43_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27017.1"
                     /translation="MINRIKRFIADFFINVPILLLSISCIFPVVWLLYSSLKTDQEFA
                     VSPVALPSRLHFENYADAFGRAKFSVFFFNSVFNSLVSLVLVLIISFVLGYILSRYRF
                     PGRNLIYMILMAGIMIPVYALIVPIFIQFKGIGLLNSRFTLIPVYVAFELPTSVFLID
                     GYLKGISVDLEEAATIDGAGMWRIMFSIMMPVCRPIMMTVVILTFMHVWNEFPFAQVM
                     INDELLKTIPIGLTYFTSQYMTQYTLLLAALSMATIPVLVVYLFFHNKVMEGMMAGAV
                     KG"
     gene            3668..4597
                     /locus_tag="PLU43_01030"
     CDS             3668..4597
                     /locus_tag="PLU43_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27018.1"
                     /translation="MISDGYDKIDPFVRMMRIKKALAMSGKWRDIDNVFTYIAQGSAE
                     FFVDGKKYVLGVGDVIIIPPFKTHVVMSLGQEPLVQFIFHFDFFETEISRKLIHRDVL
                     GQGVDYEVPESEKLTKGQVVVSSLNETDRSEIKKLYLNMYKEFSEEKDHRGRILKAYA
                     TLLLILCMRSGMQELEKRDGSNLKKSKSWVHIENAIAYINNHYTEEDLSNDTISAAIG
                     VSPNYLTRVFGDYLGVSLHKYIINLKIEKAQQMLTIGKMNITETAEKAGFSGIHAFSK
                     TFKSVIGMTPSEYMDYVAQKETIIPGGKRPDEK"
     gene            4633..>5542
                     /locus_tag="PLU43_01035"
     CDS             4633..>5542
                     /locus_tag="PLU43_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOO27019.1"
                     /translation="MWIEGNYRRNLMDMHIDDWNEEFLSKINCDEYVEALKDAGVQAA
                     MVKAKPHTGLNYFKGSVGRMHKGLKGHDFLAEMIEKCHAAGIAVEAYFTQVFDNWAYE
                     NHPEWRCIGPDGKTFREYRGAENFKTGRYGIVCPNNLGYREYVKENLQELNRNYQFEG
                     MFLDMTFWPDVCFCPSCRERYYKETGKELPRTIDWSDPEFLEYVYRREQWLADYAKFA
                     TDAVKEINPDVTIEHQFSMITAPWINASSELLMDAVDYAGGDYYGGFLQQTFINKYYK
                     NVSPNLPFIYHTSRCDPELLFHTTTKT"
BASE COUNT         1610 a         1069 c         1412 g         1451 t
ORIGIN      
        1 caggcagcca tggaccgcct gacaaaagga aggacttctt ttgtgatcgc acaccggctg
       61 tccacgataa agaatgcgga cctgattctt gtgatgaagg acggggatat tgttgaaagc
      121 ggaagtcata aggagctgct tgcaaaaggc ggcttttatg cggaacttta caacagtcag
      181 tttgaagcag cataaggagg gttgtacaga acgttccgtt ttagacattt ttaaacaaac
      241 cgcagaaaaa ataaagtgta ggaattgaca aataataata atcctgtcta aataataatt
      301 gtatattatg cattataata caaagcataa gaaacgtttc ttgttaaaaa tatcagaaaa
      361 aataagacaa aatcgaatta tgcgatgtat tgattttaat aaatcaagga gggaaaaaat
      421 gataaaaaat tataaaaaaa taatagcaat gttactgacg acagcaatga cagtctcact
      481 ttgtgcctgc ggcagctctt ccgccgaaca gacaacatct gatacaacaa cagatgcagc
      541 ggcagatacg acggaagcgg ctgaaacaga aactgccgat acggagaccg caccggcagc
      601 agaggaagca ggtacggacg gactgacagg atctttttcg atcttccatt ttcacgagga
      661 agacggagcc ggcacatcaa aagcattctg ggcggcagca aatgctttca gtgaagaaaa
      721 cccgaatgtt acgatcgatt atcagtttac ggacagtgac aattatcagg aaaagctgac
      781 aacgcttatg gcgggagacg aactgccgga tgtattcctg acaaagggcg atatgctcgg
      841 aacgcttgcg gatgcaggca tgatccagcc gcttgatcag tatattcagg ctgatgcgga
      901 gtgggctgcg gcttattcgg acggcgtatt cagcgactgt acgtatgacg gcacggaatg
      961 gggagctccg ttccagatgc aggcaaactg tattggcgtc tataatgaag aaatttttga
     1021 agaatgcggt gtcgacggct ggccggagac atgggatgaa atgcttgacg catgcgagaa
     1081 gatcaaggca aagggctata cgccgatcgc gatgggcaac aaggatcagt ggcttgcaga
     1141 atcggccgta ttcaatacct atgcttataa atatgtggac ggcgactggt ttgacagctt
     1201 aaagaacaat acaggcgcaa aattcacgga cgacaaattt gtggaagcgc tgacaggctt
     1261 tcaggagatt gcagacaaag gctatttgaa cagcgatgtc aattccatta atcaggatga
     1321 gatgtatgcc ctgtattata ataagcaggc tgcgatgttc tttaacggtg catggtcgat
     1381 cggtacactt acggaaaacg caccggaaga tgttcttgcc aatacgcatc tgggactgat
     1441 gccggcagtt tccggacagt ccggtacaaa gtatgatacg gctgcaggcg caggctggaa
     1501 ctatgtgata agagccgatc tggaaggtga tgatcttacg gctgctcttg cattcatcaa
     1561 acaggtgaca acaggcgatt atgctgacaa cgctttaagg aacgggttct tctctgcagc
     1621 aaaacaatcc ggaaatgtca gcgaatcgga attaccgaca ctgtttgccg aatattcagc
     1681 actggagtcg accatgaatt tccttccgat ctttgactgt cagcttcctg ctgaaatcgg
     1741 aagcggcgta ttgtatgcag atacgcagca gctgatcgca gatacgatca caccggagga
     1801 tatggcggct gactgccagt ccacaatgga agccaattat taagattatt aagacaagca
     1861 attcaagagt gcgtaccgga ccggtactcc gggcgcactc tttttttaaa acaggaggta
     1921 aatcagatga atgtacgtca ggtcagatcc aaaagacaat atgcgctcat ttatacatta
     1981 gtcctaattc cgtttttgta ttttttatat attttgatca ttccgttagg aacatccgca
     2041 tattacagcc tgacaaagtg gaaaggtgta ggagatccga agtttatagg actgaaaaat
     2101 tatataacac ttgttcatga cagcaatttc tggcttgtga caaaaaatac ggtcatattg
     2161 tcactgctgt gtgtactcgg gcaggtcggg atcgcactga tcattgcatt tctgatgacg
     2221 atccgaagat taaaatggaa atcctttcac agggcagtca tcttttttcc ggtcgtaatg
     2281 gcgccgatcg tagtaggcta tgtgtggcgt tttatttaca attccaatta cggattactg
     2341 aatatgtttt taaatgcaat cgggaaatct tcctggataa aaaactggct ggatgatcat
     2401 gatattgtgc tgcggatggt ttcaattccc gtaatatggc agtatatcgg actttatatg
     2461 atcctgctga tgagcggtat ttcggcgata ccaacagagg tgttcgaatg tgctgagatc
     2521 gacggggcca caggtctgaa gaaatctttg tatatcacgc ttcccctgat ctgggataca
     2581 ctcaaaatat gcatcattct ctgcgcatcc gggacaatga aaatttacga tcatctggtt
     2641 gcactgacaa acgggggacc cggaaaagct tcctcttcac ttgctctgta ttgctatgac
     2701 tacacgttta aattcggcaa ttttgggatg ggggcggcga tcgctgttgt gatcctgctg
     2761 cttgcggtcg gtatcagcct gcttgtccag ctgctgatgg gaagaaagga ggcctagcat
     2821 gataaacagg atcaaacgct ttatagcaga tttttttata aatgtaccaa tattgctgct
     2881 ctccatttcc tgtatttttc ctgttgtatg gctgctgtat tcgtcattga aaacagacca
     2941 ggaatttgcg gtcagtccgg ttgcacttcc ttccaggctg cattttgaga attatgcgga
     3001 tgcgttcgga cgtgccaaat tcagtgtgtt cttttttaac agtgtcttca acagcctggt
     3061 atcccttgtt ttggtactga tcatttcatt cgtactcgga tacattttgt cccggtaccg
     3121 ttttccggga agaaatctga tctatatgat attgatggca ggaatcatga ttcctgtgta
     3181 tgcgctgatc gtaccaattt ttatccagtt taagggcatc ggcctgctga acagcagatt
     3241 tacgctgatt cctgtctatg tggcgttcga actgccgacg tcggtatttt tgatcgacgg
     3301 gtatttaaaa gggatctccg tggatctgga agaagcggca acgatcgacg gcgcgggtat
     3361 gtggcggatc atgttttcca ttatgatgcc ggtctgccgg ccgatcatga tgactgttgt
     3421 gatactgaca ttcatgcatg tctggaatga gtttccgttt gcgcaggtca tgatcaatga
     3481 cgagctttta aagacgatac cgatcggtct tacatatttt acttcgcagt atatgacgca
     3541 gtatacgctg ctgctggcag cactgtccat ggcaacgata ccggttctgg tcgtttatct
     3601 gtttttccat aataaagtca tggaaggcat gatggccggc gctgtaaaag gataacgagg
     3661 tgtttggatg atttccgatg gatatgacaa aatagatccg tttgtcagga tgatgagaat
     3721 caaaaaggca ttggcaatga gcggaaaatg gagagatatt gacaatgtgt ttacatacat
     3781 tgcgcagggc agcgccgaat tttttgtcga cgggaaaaag tatgtcctgg gtgtcgggga
     3841 tgtgatcatt attcctcctt ttaagacgca tgttgtcatg tcgctcggac aggaaccgct
     3901 tgtgcagttt atttttcatt ttgatttttt tgagacggag atcagcagga aactgatcca
     3961 tcgggatgtt cttggtcagg gtgttgatta tgaagttccg gaaagcgaaa agctgacaaa
     4021 agggcaggtc gttgtttcct ctttgaatga aacagacagg tccgagatca aaaagctgta
     4081 tctgaatatg tataaagaat tttcggaaga aaaggaccac agaggaagaa tattaaaagc
     4141 gtatgcgacg ctccttctga tcctgtgtat gagaagcggc atgcaggagc tggagaagcg
     4201 cgacggcagc aacctgaaaa agtcaaagtc atgggttcat atagaaaatg cgatcgcata
     4261 tatcaacaat cattacacgg aagaagatct aagcaatgat acgatcagcg ctgcgatcgg
     4321 tgtttcccca aattatctga caagagtttt cggcgattat ctgggggttt cgcttcacaa
     4381 atatattatc aatttaaaga tcgaaaaggc gcagcagatg ctgaccatag ggaagatgaa
     4441 cattaccgag acagcggaaa aggccgggtt ttcaggaatc catgcattca gcaagacatt
     4501 taaatccgta ataggcatga caccgagcga atatatggat tatgtcgcac aaaaagaaac
     4561 gattattcca ggcggaaaga gacctgacga aaaataataa aataatgagc agggcataga
     4621 aagaggttaa acatgtggat agaaggaaat tacagacgca atttgatgga catgcatatt
     4681 gacgactgga atgaagagtt tttgtcaaag atcaattgcg atgaatatgt ggaagccctg
     4741 aaagatgcgg gcgttcaggc cgcaatggta aaggcaaagc cgcatacggg cctgaattat
     4801 tttaagggtt ctgtaggaag aatgcataaa ggcttaaagg gacatgattt tctggcggag
     4861 atgatcgaga agtgccatgc tgccggaatc gccgtagaag cttatttcac acaggtattt
     4921 gataactggg cctatgaaaa tcatccggaa tggagatgta tcggtccgga tggaaaaacg
     4981 ttccgggaat accgcggggc ggaaaatttt aaaacgggac gctatggaat cgtctgcccg
     5041 aacaatttgg gataccggga atacgtcaaa gaaaatctgc aggagctgaa caggaattat
     5101 cagttcgaag ggatgtttct ggatatgact ttttggcctg atgtctgctt ctgcccaagc
     5161 tgcagggaaa ggtattacaa ggaaacaggg aaggagcttc caaggacgat cgattggagt
     5221 gatccggaat ttttagagta tgtatacaga agagaacagt ggctggccga ttatgcaaaa
     5281 tttgcgacag acgccgtaaa ggagatcaat ccggacgtga cgatcgagca tcagttttcc
     5341 atgatcacgg cgccctggat caatgccagc agcgaactgc tgatggatgc ggtcgattat
     5401 gcgggcggtg attattacgg cgggttttta cagcagacgt ttatcaacaa atattataag
     5461 aatgtatcgc cgaatcttcc gtttatttac catacgtcca gatgtgatcc ggagcttctg
     5521 ttccatacaa caacaaagac aa
//
LOCUS       DAOSYE010000031         5529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_9801_length_5529_cov_3.320058, whole genome shotgun sequence.
ACCESSION   DAOSYE010000031 DAOSYE010000000
VERSION     DAOSYE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_5529_cov_3.320058"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLU43_01040"
     CDS             complement(<1..394)
                     /locus_tag="PLU43_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOO27020.1"
                     /translation="MTKTFDDLLKKLSECSKKKVAVAAAQDAPILEAVSEAKKRGIAD
                     AILVGDEDKIKEIALSLHMDLSEYEIIDEKDMEQAALTAVGLVHDGKADMYMKGLTDS
                     KTFLKSVLNKEVGLRTGKPLSHVCVFEIE"
     gene            613..3177
                     /gene="secA"
                     /locus_tag="PLU43_01045"
     CDS             613..3177
                     /gene="secA"
                     /locus_tag="PLU43_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOO27021.1"
                     /translation="MKIIDKIFGTHSERELKLIEPLVDKVEALRPEMMALSDAALREK
                     TKEYKKRLADGETLDDLLPEAYATVREAARRVLNMEHYPVQIVGGIILHQGRIAEMKT
                     GEGKTLVSTLPAYLNALEGKGVHIVTVNDYLAKRDAEWMGQVHEFLGLTVGVVLNEMK
                     ADMRKAAYACDITYVTNNELGFDYLRDNMVIYKEQLVLRELAYAIIDEVDSVLIDEAR
                     TPLIISGQSGKSTKLYEVCDILARQLKRGEDVEDLTKMDAIMGIEREETGDFLVNEKD
                     KFVTLTAEGVEKVEKFFQIENLADPENVDIQNNMILALRAHNLMFRDQDYVVKDDQVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKEHVKVKRESKTLATITFQNFFNKFEKKCGMTG
                     TALTEEKEFRDIYSMDVIEIPTNRPIARTDLQDAVYKTKKEKLLAICNEIEKAHATGQ
                     PILVGTITIEASEELSILLKRRGIQHKVLNAKFHELEAEIVADAGIHGAVTIATNMAG
                     RGTDIKLDKESRAAGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESRFFISLEDDLM
                     RLFGSDRLMEVFNALGVPENEQIEHKMLSSAIEKAQMKIENNNFGTRKNLLEYDQVMN
                     EQREIIYAERRRVLDGESMRDVIFKMATDFVENTVDMCIGDDTIPEEWDLPEINQHLL
                     ATVPVKELKTPDIRHLKKNELKHNLKEEVVKLYEAKEAEFPMPEQLRELERVVLLKSI
                     DRKWMDHIDDMDQLRQGIGLQAYGQRDPLVEYKMTGYDMFNAMTDSISEETVRVLLHI
                     RIEQKVEREEVAKVTGTNKDTSLGKKPQKRESVKIYPNDPCPCGSGKKYKQCCGRK"
     gene            3217..4359
                     /gene="prfB"
                     /locus_tag="PLU43_01050"
     CDS             3217..4359
                     /gene="prfB"
                     /locus_tag="PLU43_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOO27022.1"
                     /translation="MVELDQIKTELQTYQEPLKEVKASLSLEDKEKRIEELEREMEAP
                     SFWDNPERSQKYVKELKNYKDTIELVSGLEQQYEDIGMLLQMGYEENDPELVADIRKE
                     FDEFKETLENLRIATLLSGEYDKNNAILRLNAGAGGTESCDWAGMLYRMYSRWAEKKG
                     FSVEVLDYLDGDEAGIKSVTFQINGENAYGYLKSEKGVHRLVRISPFNAQGKRQTSFV
                     SLDVMPAIEEDLDIDINEEDLRIDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQN
                     ERSQFQNKDKAMQMLKAKLYLLKKEENAEKLSDIRGDIKEIGWGNQIRSYVMQPYTLV
                     KDHRTNAEAAQVDSVLDGNLDLFINAYLKWINTGGELQKGREAFEI"
     gene            4499..4942
                     /locus_tag="PLU43_01055"
     CDS             4499..4942
                     /locus_tag="PLU43_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO27023.1"
                     /translation="MSETNAYYIVKQKALPEVLLKVVEAKTMLDSERAMTVQEATEQV
                     GISRSSFYKYKDDIFRFHDNTQGKTITLTVQMDDRPGLLADLLKIIAAFGANILTIHQ
                     SIPINAVASLSISVQVLPATGDIAVMIESMEKQEGVHHVKILAKE"
     gene            4981..>5529
                     /locus_tag="PLU43_01060"
     CDS             4981..>5529
                     /locus_tag="PLU43_01060"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOO27024.1"
                     /translation="MVNIAVLGYGTVGSGVVEVIHTNPDSINKKAGEEINIKYVLDLR
                     NFPGDPVEKVLVHDFDVILNDPEVKIVVEVMGGVEPAYTFVKKCLLAGKSVCTSNKEL
                     VAKHGPELLQIARDNSINFLFEASVGGGIPIIRPLNSSLTADEILEITGILNGTTNYI
                     LSKMSDEGWEFDEALKKAQELGY"
BASE COUNT         1663 a         1007 c         1476 g         1383 t
ORIGIN      
        1 cttcgatttc aaacacgcat acatgtgaca gcggttttcc ggtacggagt ccgacttctt
       61 tgttcagaac actttttaaa aaggttttgc tgtctgtcag tcctttcatg tacatgtctg
      121 ccttgccgtc atgtacgagt ccgactgcag tcagggcggc ctgttccata tctttttcgt
      181 caatgatttc atattctgaa agatccatat gcagggaaag cgcgatttct tttattttat
      241 cttcatcacc aacaagaata gcgtcggcaa taccgcgttt ctttgcttca ctgacagctt
      301 caagaatcgg agcatcctgt gcggctgcaa cggcaacctt tttcttactg cactctgaca
      361 gctttttcag taaatcatca aatgtttttg tcattttaca cacctctggt atttattaaa
      421 tttttcttta tgcggaattc gttaaaataa taacactttt cagctgaaaa agcaattgtt
      481 tactgttttt taatacaaag aaggttgaaa gcatgttcca attctgttac aatacaattt
      541 aggtcatttt actaagatta tgtgaaaaat gtgattaaat ttgttgatca ttggtaaaca
      601 ggagcataga caatgaaaat aattgacaag atattcggaa cccatagtga acgggaactg
      661 aaactgatcg aaccgcttgt agataaagtg gaggctcttc gcccggagat gatggctttg
      721 tcggatgccg cactcaggga aaagacaaag gaatataaaa agagactggc tgacggggaa
      781 acactggatg atcttttgcc ggaggcttat gcaacagtac gtgaggcggc aagaagggtc
      841 ctgaatatgg aacactatcc ggtgcagatc gtgggtggta ttattctgca tcaggggcgt
      901 attgccgaaa tgaagacagg tgaaggaaag acgctcgtat ccacgctgcc ggcttattta
      961 aatgcgctgg aagggaaggg cgtgcatatt gtgaccgtca atgactatct ggcaaagcgt
     1021 gatgccgaat ggatgggaca ggtacacgaa ttcctcggac tgactgtcgg tgtcgtactg
     1081 aatgagatga aagccgatat gaggaaagca gcttatgcct gcgatatcac ttatgtgaca
     1141 aataatgagc ttggttttga ttatctgcgt gataatatgg tgatttataa agaacagctc
     1201 gtacttcgcg agctggctta tgcgatcatt gatgaggtcg attccgtttt gatcgatgag
     1261 gcgagaacac cgcttattat ttccggacag agcggcaaat ccacgaagct ttacgaagtc
     1321 tgcgatattc tggccagaca gctgaagcgg ggcgaggatg tcgaagacct gactaaaatg
     1381 gatgccatta tgggaatcga acgggaagag accggtgatt ttcttgtaaa tgaaaaggac
     1441 aaattcgtaa cgctgacggc agaaggtgtg gaaaaggtcg aaaaattctt ccagatcgaa
     1501 aatcttgccg atccggaaaa tgtagatatc cagaataata tgatccttgc attgcgggca
     1561 cataacctga tgtttcgaga tcaggattat gtggtaaagg atgatcaggt tctgatcgta
     1621 gacgagttta cgggacgtat tatgcccgga cgccggtatt cggatggtct gcatcaggcg
     1681 atcgaggcaa aggaacatgt taaggtaaaa agagaaagca agacgcttgc gacgatcaca
     1741 ttccagaact ttttcaataa atttgagaaa aaatgcggta tgaccggtac ggctcttacg
     1801 gaagaaaagg aattccgtga tatttacagt atggatgtaa ttgagattcc gacaaacaga
     1861 ccgatcgcca ggaccgatct gcaggatgcc gtatataaga caaaaaagga aaagctgctt
     1921 gccatctgca atgagatcga gaaagcccat gctacgggtc agccgatcct ggtaggcacg
     1981 attacgatcg aggcttctga ggagctgagc atcttattaa agaggcgggg gatccagcat
     2041 aaggttctga atgcgaagtt ccatgagctg gaagcagaga tcgtggctga tgcgggtatt
     2101 cacggcgcgg ttacgatcgc gacgaatatg gccggccgtg gtacggatat taaactggat
     2161 aaggaatcaa gggcagccgg cggacttaag atcattggta cggagcgtca tgaatccaga
     2221 cgaatcgata accagctgcg cggccgttcc ggccgtcagg gagatccggg tgaatccagg
     2281 ttctttattt cccttgaaga tgatctgatg cgtctgtttg gttccgatcg tctgatggaa
     2341 gtgttcaatg cacttggtgt gccggaaaac gaacagatcg agcataagat gctttcaagt
     2401 gcgatcgaga aagcacagat gaaaatagaa aacaataact ttggtaccag aaagaatctg
     2461 ctggaatatg atcaggtcat gaacgaacag agggagatca tttatgcaga aagacggcgt
     2521 gtacttgacg gagaaagcat gcgtgacgtt attttcaaaa tggcgactga ttttgttgaa
     2581 aatacagtgg atatgtgtat tggagatgat acgattccgg aagaatggga tctgccggag
     2641 atcaatcagc atctgcttgc gactgtgcct gtaaaagaac tgaagacgcc ggatatccga
     2701 catctgaaga aaaatgaact gaagcataat ttaaaagaag aagtagtgaa attatatgaa
     2761 gcaaaagaag cggagttccc gatgccggag cagcttcgtg aactggaacg ggtcgtattg
     2821 ctgaaatcga tcgaccgcaa gtggatggac catattgacg atatggatca gctgcgtcag
     2881 gggatcggtc tgcaggctta cggacagaga gatccgcttg tagaatataa aatgacagga
     2941 tatgatatgt tcaatgcaat gacggacagc attagtgaag aaacagttcg tgttctgctt
     3001 catatccgta ttgaacagaa ggtagaaaga gaagaggttg ctaaggtgac cggcacaaat
     3061 aaggacacat cacttggaaa gaagccgcag aagagagaat ctgtgaaaat ctatccgaat
     3121 gatccatgtc cgtgcggaag cggtaagaaa tataaacagt gctgcgggcg caagtaatgg
     3181 tcaaaagagc cggcagcagc ggaaacggag atttttgtgg tagaattaga ccagataaaa
     3241 acagaattac agacatatca ggagcctttg aaagaagtaa aggcttcttt gtcgctcgag
     3301 gataaggaaa aacggatcga ggaactggag cgtgaaatgg aagcgccgag tttctgggat
     3361 aatccggagc gttctcaaaa gtatgtgaag gaactgaaaa attataagga tacgatagag
     3421 ctggtgtcag gcctggagca gcagtatgaa gatattggga tgcttctgca gatgggttat
     3481 gaggaaaatg atccggagct ggttgcagat atccggaaag aatttgacga gtttaaggaa
     3541 acacttgaaa atctgcggat tgcaacattg ctttccggcg aatatgataa aaataatgcg
     3601 atcctgcgtc tgaatgccgg agcaggcgga acggaaagct gtgactgggc agggatgctt
     3661 tatagaatgt attcacgctg ggccgaaaaa aaaggttttt ctgtggaagt actcgactat
     3721 ctggacggag atgaggcggg catcaagtcc gttacgtttc agataaacgg tgaaaatgcc
     3781 tacggctatt taaaatccga aaagggcgta caccgtcttg tgcgcatttc tccgtttaat
     3841 gcgcagggaa agcggcagac atcgttcgta tctcttgatg taatgcctgc gatcgaagag
     3901 gatctggata ttgatatcaa cgaggaggac ctgcgtatcg atacataccg aagttccggt
     3961 gcgggcggac agcatatcaa taaaacatcc tctgctattc gtattacgca tcttccgacc
     4021 ggtatcgttg tgcagtgcca gaacgagcgg tcccagtttc agaataaaga caaggcaatg
     4081 cagatgctga aggcaaagct gtatctttta aagaaagaag aaaacgctga aaagctgtcc
     4141 gatatccgcg gggatatcaa agagatcggc tggggaaatc agatccgttc ctatgtgatg
     4201 cagccgtata cgcttgtcaa ggatcacagg acaaatgcgg aggcagcgca ggtggacagt
     4261 gtgctggacg gtaatcttga cctgtttatc aatgcctatt taaagtggat caatacgggt
     4321 ggtgagttac agaaaggcag ggaggcattt gagatataat ttaaaaaagc aggtatttta
     4381 ggataaatac ttgctttttt taatacgtta tgttacgata tctttcagcg aaagacacgt
     4441 gttcgcacca tgaatacaaa acaggatgtg tgttcattca acaaggacag gtgatactat
     4501 gtcagaaacg aatgcttatt atatcgtaaa gcagaaagcg cttccggaag tgctgctaaa
     4561 ggttgtggag gcgaaaacga tgctggactc cgaacgtgcc atgacagtgc aggaggctac
     4621 ggaacaggtg ggaatcagca ggagctcctt ttataaatac aaggatgata tttttcggtt
     4681 ccatgacaat acacagggca agacgattac attgacggtt cagatggatg accgtcccgg
     4741 actgctggct gatctgctga agatcatcgc ggctttcgga gccaatattc tgacaattca
     4801 tcaaagtatt ccaatcaatg ccgttgcatc tttaagtatc agtgtgcagg tgctgcctgc
     4861 gacaggtgat attgccgtaa tgatagagag tatggagaag caggaaggtg ttcatcacgt
     4921 aaagatttta gcaaaagaat aaaatggata atagaaaata tgagataaag gagaataatt
     4981 atggtaaata ttgcagtgct tggatatggt acggttggtt cgggagtagt agaagtcata
     5041 cataccaatc cggacagtat taataaaaag gccggagaag agatcaacat caaatatgtt
     5101 ctggatttga ggaattttcc gggtgacccg gtagaaaaag tgcttgtaca tgattttgat
     5161 gtgatactga acgatccgga agtgaagatc gtagtggagg tcatgggcgg tgtcgagccg
     5221 gcctatacgt tcgttaaaaa gtgtctgctt gccgggaaga gcgtctgcac ttccaataag
     5281 gagcttgttg cgaagcatgg tccggagctt ttgcagatcg caagggacaa cagcattaat
     5341 ttcctgtttg aagcaagtgt cggcggtgga attccgatca tccgtccgct taactcttcc
     5401 ctgacggctg atgaaattct tgaaattacc ggtattctga atggtacgac aaattatatc
     5461 ttaagcaaga tgtccgatga gggctgggaa tttgacgagg cgctgaaaaa ggcacaggag
     5521 cttggctat
//
LOCUS       DAOSYE010000032         5443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_10025_length_5443_cov_2.850780, whole genome shotgun sequence.
ACCESSION   DAOSYE010000032 DAOSYE010000000
VERSION     DAOSYE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10025_length_5443_cov_2.850780"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLU43_01065"
     CDS             <1..628
                     /locus_tag="PLU43_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27025.1"
                     /translation="KEVLKIHNQKMNKEELNQAVDDMMELVGIPANRKNEYPHEFSGG
                     MKQRIVIAIALVCEPKLILADEPTTALDVTIQAQMLGLINNLQKRLNTAMILVTHDLG
                     VVAQTCEKVAVMCAGEVIEIGTARHIFERRLLHPYTQGLFSAIPKLDATAHRLETIEG
                     AVPDPTVKLKGCRFAERCKHATDQCTCVPDMIEVEEGHYIKCHLFKKG"
     gene            655..1608
                     /locus_tag="PLU43_01070"
     CDS             655..1608
                     /locus_tag="PLU43_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27026.1"
                     /translation="MEEKTLIEVRNLKKYFKTSKGMLHAVDGISFKIKKGTTLGVVGE
                     SGCGKSTLGRVLLHLHEATDGQIFYDGVDITNYDAKQLVEIRKKMQMIFQDPSASLDP
                     RKTVKELIGEPLKNYKVYKTKKEYEDRVWEIMKTVGLSERYADSYSHELDGGRRQRIG
                     IGRALAMDPEFIVCDEPVSALDVSIQAQVLNLLMDLQDEKGLTYVFITHDLSVVKHIS
                     HEIAVMYLGQCVEYAKTEELFKNPLHPYTKGLLEAVPIPVLTEKKLGAEVMKGELTSP
                     IEPKPGCRFANRCPFASEKCRNNDIPLKEVEPGHFAACTLF"
     gene            1649..3349
                     /locus_tag="PLU43_01075"
     CDS             1649..3349
                     /locus_tag="PLU43_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOO27027.1"
                     /translation="MKTQEEAAIQRVREEFKKCGTLPFCRDWTSEETMLAMEDGVSLR
                     TLICRPVRQGAVPTIVMRTCYTENDPVYRVTAEEYCKRGFAYIYQYCRGTGGSEGEWE
                     PNVNERKDGKTTIDWICAQDWVGNTGYFGCSYLALTGWTIADILPPKVKTMYLTHYGV
                     HRFTSVYKDGLFRHDVLTAWSMGNAGTKIDADYLESCRYRPQEEVDEALWGVRLNWYR
                     EWITNTNRTDKYWNTGFWKMLKEIPKKVTIPLYIGEGWYDHHLGSAIETWLTLSEESR
                     KKSCLLIGAWDHGYNIKLQDRTGSHFDNNDTVRAFEWFQKLLEEEAEPAGCVTYYVIG
                     GDAWRTEKQFEVKDRKEARLYIGEQKCGKSFGLLPVNGACRGEADFLYDPDDPVMTHG
                     AESLLATQDEQGSLLQQVPGYRNDVISCVSEPFENDMIIFGKINVNLSVSTDADDTSF
                     AVKIMEVTEDGNAYNIRTGITTLGYRNNSDTRISYAPGEKVSVTIDTWDIAWKIKKGS
                     RLRLDITSSDFPQYSIHTNYAGCWAKQKEVRTARQHIYFGGADQSYISLPYIADSNND
                     "
     gene            3379..4428
                     /locus_tag="PLU43_01080"
     CDS             3379..4428
                     /locus_tag="PLU43_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOO27028.1"
                     /translation="MKNTMKAAIMTGIEKVELTERKIPQPKENEVLVKIHHVGICGSD
                     LHYYEQGAIGNYVVKPPFVLGHEAAGTVVEVGSKVTHLKKGDRVALEPGKTCGECEFC
                     KAGKYNLCPDVVFFATPPVDGVFQEYVAHEAGLCFKLPEQMDTVEGALIEPLAVGFHA
                     GLLGGAKIGQTAVVAGAGCIGLVTLLALRAMGVSKIIVVDIMQNRLEKALELGADEII
                     NGKEENVIERVAELTEGKGCDLGIETAGNEITSKQLIHVAKKGSTIVFVGYSASGEVT
                     LPMSLALDKELTFKTVFRYRNIYPMAIEAVASGKINLKSIVTNYFDLDHIQEGLQACI
                     KDKANIVKGVIKISD"
     gene            4503..>5443
                     /locus_tag="PLU43_01085"
     CDS             4503..>5443
                     /locus_tag="PLU43_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HOO27029.1"
                     /translation="MKKIAIGGGQGFWGDSPDAAIHLIKKGNIQYLSCDYLAELTLSI
                     MAKQQLKNPAKGFAPDFASRILKAAGKEAWEKNIKMCTNAGGMNVTGCVDQIREWAES
                     ENLSGYKIGYVTGDSIKDKIPQLLKDGWTFPNFDYDGDFNEIVDSIYNCNAYIGREGI
                     EQCLADGADVVVTGRAADSALFLAPLAYEFGWRDDDWDNLARGIMAGHLLECGGQGSG
                     GNYMYDWKNVPRMDELGFPIAELTPDTFEITKAPDCGGVICEQSCKEQFLYEVLDPSN
                     YVTPDVNVDVSQATIKQVGEDRVRIGNIHGKKKPDTL"
BASE COUNT         1668 a          986 c         1528 g         1261 t
ORIGIN      
        1 caaagaagta ctgaaaatac ataatcagaa aatgaataag gaagaattga atcaggcagt
       61 agacgatatg atggagcttg tcgggattcc ggcaaacaga aaaaatgagt atccgcatga
      121 attttcaggc ggcatgaaac agcgtatcgt aatcgcaatt gcacttgtat gtgaaccgaa
      181 gctgattttg gcggatgaac cgacaacggc attggacgtt acgatacagg cgcagatgct
      241 tggactgatc aacaatctgc agaaacggct gaatacggca atgattcttg ttacacatga
      301 tctcggagtt gtggcacaga catgcgaaaa ggtagctgtc atgtgtgcgg gtgaagtgat
      361 cgagatcgga acagcccggc atatatttga aagacgattg ctgcatccgt acacgcaggg
      421 actgttctct gccattccga aactggatgc gactgcacat cgactggaaa cgatcgaagg
      481 cgcggtaccg gatccgaccg ttaaactgaa gggctgccga tttgcagaga gatgtaagca
      541 tgcaaccgat cagtgtacct gtgtaccgga tatgatcgag gtagaagaag ggcactatat
      601 caaatgtcat ttgttcaaaa agggataggt tgacattacc ggaggaaata tcagatggaa
      661 gaaaaaacac tcatagaagt gagaaactta aaaaaatatt ttaaaacatc caaaggtatg
      721 ctgcatgccg tagacggtat cagttttaaa ataaaaaaag gaacgacact cggcgttgta
      781 ggagaatccg gatgcgggaa gtctacgctt ggaagagtac tgctgcattt acatgaggca
      841 acggatggtc agatctttta tgacggtgtg gatattacaa attatgatgc aaaacagctg
      901 gtggaaataa gaaaaaaaat gcagatgatc tttcaggatc catccgcatc acttgaccca
      961 agaaaaacag taaaggaatt gatcggcgag ccgctgaaaa attataaagt ttacaagaca
     1021 aaaaaagagt atgaagacag agtatgggag atcatgaaga cagtcggatt gtcggaaaga
     1081 tatgcggaca gctattctca tgagctggac ggcggcagga ggcagaggat cggtatcggc
     1141 cgggcactcg ccatggatcc ggaatttatt gtctgtgacg aaccggtttc ggcgctggat
     1201 gtttccatac aggcacaggt attgaatctg ctgatggatc tgcaggatga aaagggactg
     1261 acctatgttt ttattacaca tgatttgagt gtcgtaaagc atatcagcca tgaaatagcc
     1321 gtgatgtacc tggggcagtg tgtggagtac gcgaaaacag aagaattgtt taaaaatcca
     1381 ctgcatccgt atacaaaggg tctgcttgaa gctgttccaa ttcctgtcct gacagaaaag
     1441 aaactgggag ctgaagtcat gaagggtgag ctgacaagcc cgatcgagcc aaaaccaggg
     1501 tgcaggtttg caaacagatg tccgtttgcg agtgagaagt gcaggaataa tgatattccg
     1561 cttaaagaag tagagcccgg acattttgct gcctgtacgt tattttaaag gccaggatgt
     1621 ctgatctata aaaacaagag gtgatttggt gaaaacacag gaagaagctg caatacaaag
     1681 agtcagagag gaatttaaga aatgcggaac gcttccgttc tgcagggact ggacgtctga
     1741 agagacaatg ctggcaatgg aggacggtgt ttccctgcgc acgctgatct gcagaccggt
     1801 acggcaaggg gcggtaccga cgatcgtgat gaggacctgc tatactgaga atgatcctgt
     1861 ataccgggtg acggctgaag aatattgcaa aaggggcttt gcctacattt atcagtactg
     1921 caggggaaca ggcggctctg aaggagagtg ggaaccgaat gttaacgaaa gaaaagacgg
     1981 aaagacgaca atagactgga tatgtgcaca ggactgggtt ggaaataccg ggtatttcgg
     2041 gtgttcttat cttgcgctga ccggatggac gattgccgat attctgccgc caaaagtaaa
     2101 aacaatgtat ctgacacatt acggagttca tcggtttacc tccgtatata aagatggact
     2161 gttccgtcat gacgtattaa cggcatggtc tatgggaaac gcaggaacaa agatcgatgc
     2221 cgattatctg gaatcctgcc gttacagacc gcaagaggaa gttgatgagg cactttgggg
     2281 cgtacggctg aactggtaca gagaatggat cacgaataca aacaggaccg ataagtactg
     2341 gaatacgggg ttctggaaaa tgcttaagga aattccgaag aaggtaacga ttcctcttta
     2401 tatcggggaa ggctggtatg atcatcatct tggctccgca atcgaaacct ggctgacgct
     2461 gtctgaggaa agcagaaaaa aaagctgtct tctgatcggc gcatgggatc atggatataa
     2521 tataaagctg caggatcgaa ccggcagtca ttttgacaat aatgacacgg tacgtgcatt
     2581 tgaatggttt caaaagcttt tggaagagga agcggaaccg gcagggtgtg tcacctatta
     2641 tgtgattggt ggggatgcct ggagaacaga aaagcagttt gaagtaaagg accgaaaaga
     2701 ggcaaggctg tatatcggag agcagaaatg cggaaagtca tttggactgc tgccggtaaa
     2761 cggagcctgc cggggagagg cagatttcct atacgatcct gatgatccgg ttatgacgca
     2821 tggggcggaa tctcttcttg ctacacagga tgaacaggga agtcttttgc agcaggtgcc
     2881 gggataccga aatgatgtga tcagctgcgt ttcagagccg tttgaaaacg acatgattat
     2941 ttttggcaaa ataaatgtga atttatcggt atcgacagat gccgatgata cgtcttttgc
     3001 ggtgaagatc atggaagtaa cagaggacgg aaacgcttat aatatacgga cgggtataac
     3061 gacgctcgga tacaggaata acagtgacac gagaatttcc tatgccccag gcgaaaaggt
     3121 ttctgtaaca atcgacacgt gggatattgc atggaagata aagaaaggtt cgaggctcag
     3181 gctcgatatt acatcttccg attttccgca gtattccatt catacaaatt atgccgggtg
     3241 ctgggcaaaa cagaaagaag tgaggactgc caggcagcat atatattttg gcggtgccga
     3301 ccagtcatac atcagtctgc cgtatattgc tgacagtaat aacgattaac ggggaaaaga
     3361 tcacggaagg agaaaactat gaaaaatact atgaaagctg caataatgac agggatcgaa
     3421 aaggtagaac ttacggaacg aaagatcccg cagccaaaag agaatgaagt attggtaaag
     3481 atccatcatg tgggcatctg cggttcggat ctgcactatt acgagcaggg agctatcggg
     3541 aattatgttg taaagccgcc gtttgtgctt ggacacgaag cagcgggtac ggtagtggaa
     3601 gtcggaagta aggtgacaca tttaaaaaag ggagacagag ttgccttgga gcccggaaag
     3661 acatgcgggg aatgtgaatt ttgcaaagcc ggaaaatata atctgtgtcc tgatgttgtc
     3721 ttttttgcaa caccgcctgt tgacggagtt tttcaggagt atgttgccca tgaggcaggc
     3781 ctgtgtttta agctgccgga acagatggat acggtagaag gagcattgat cgagccgctg
     3841 gcagttggat ttcatgctgg gctgctcggc ggagcaaaga tcggccagac cgcagttgtg
     3901 gcaggagccg gctgtattgg tcttgtgaca ctgcttgcgc tgcgggcaat gggtgtttca
     3961 aaaataatcg tggttgatat tatgcagaac agacttgaga aggcgctgga actcggagcc
     4021 gatgagatca ttaacggcaa agaggaaaat gtgatagaaa gagtagcgga actgacggaa
     4081 ggaaagggct gcgaccttgg tattgaaacc gcagggaatg agattacttc aaaacagctg
     4141 atccatgttg caaagaaagg atccacgatc gtatttgtcg gctacagtgc atccggagag
     4201 gtaacgctgc ctatgagtct tgccctggat aaggagctga cgtttaaaac ggtcttccgt
     4261 tacaggaata tttatccgat ggcgatcgaa gcagtcgcat ccggaaaaat aaatttaaaa
     4321 agtattgtaa caaattattt tgatctggat catattcagg aaggactgca ggcgtgtatc
     4381 aaggataaag ccaatatcgt aaaaggtgtg atcaaaatct ctgactgact tacattacaa
     4441 aaaggaagag ttttcatata aagtacaacg ttgtataaaa taaaatcggg aggaaaaaca
     4501 acatgaaaaa gattgcgatc ggaggaggac agggattttg gggagattcg cccgacgcgg
     4561 ctattcatct gatcaaaaag ggaaatattc agtatttgag ctgtgattat cttgcggaac
     4621 ttacgctttc cattatggca aaacagcagc tgaaaaatcc ggcaaagggc tttgcgcctg
     4681 attttgcctc aagaattttg aaagctgcag gaaaggaagc gtgggagaaa aatattaaaa
     4741 tgtgtacgaa cgccggcggt atgaatgtaa ccggctgtgt agaccagatc agggaatggg
     4801 cggagtcgga aaacttaagc ggatataaga tcggctatgt tacaggagat tcgatcaagg
     4861 ataagatccc gcagctgtta aaggacggct ggacatttcc taattttgat tatgacggtg
     4921 attttaacga gatcgttgac agtatttata attgtaatgc ctatatcggt cgtgaaggga
     4981 tcgagcagtg ccttgcagac ggtgcggatg tggtagtgac cggacgagct gcggacagtg
     5041 ctctcttttt ggcaccgctg gcttatgagt ttggctggcg cgacgacgac tgggataatc
     5101 ttgcacgcgg aattatggca gggcacctgc ttgaatgcgg cggtcagggt tcgggcggaa
     5161 attatatgta tgactggaaa aatgtcccgc ggatggacga gcttggattc ccgattgcag
     5221 aactgacacc ggacactttt gagatcacaa aggcgcccga ctgcggcggg gtgatctgtg
     5281 aacagagttg taaagaacag tttctgtatg aagttttgga tccgagcaat tatgtaacgc
     5341 cggatgtcaa tgtggatgta agtcaggcta ccatcaaaca ggtcggagag gacagggtca
     5401 ggatcggcaa tatacatgga aagaaaaagc cggatacgct gaa
//
LOCUS       DAOSYE010000033         5174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_10741_length_5174_cov_3.336589, whole genome shotgun sequence.
ACCESSION   DAOSYE010000033 DAOSYE010000000
VERSION     DAOSYE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10741_length_5174_cov_3.336589"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="gguA"
                     /locus_tag="PLU43_01090"
     CDS             complement(<1..1020)
                     /gene="gguA"
                     /locus_tag="PLU43_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO27030.1"
                     /translation="MAKILLEMKNITKTFPGVKALDNVNFKVEEGEIHALVGENGAGK
                     STLMNVLSGTYPYGTYEGDIIYDGEVCKFFKIKDSEEKGIVIIHQELALIPYMTIGEN
                     MFLGNERGKKAAIDWTETYGRADELLKIVGLEESSRTLIKDIGVGKQQLVEIAKALAK
                     NAKLLILDEPTSSLNEEDSRALLDLLLTFKEHGMTSIIISHKLNEISYVADTITILRD
                     GATIETLDKATDDVSEGRIIKGMVGRDLTDRFPKREQKNIGEISMEIKNWNVYHPVYT
                     ERKVVDDVSLNVHKGEVVGISGLMGAGRTELAMSVFGKSYGTNISGTLYINGKEMHFH
                     NVKDAI"
     gene            complement(1116..2291)
                     /gene="chvE"
                     /locus_tag="PLU43_01095"
     CDS             complement(1116..2291)
                     /gene="chvE"
                     /locus_tag="PLU43_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO27031.1"
                     /translation="MKKKLLSVVLSAAMVAALLVGCGSTATEEAAPADDTAAATEEAA
                     PADDTAAAEEEAPAAEEETAASGGLIGVEMPTKDLQRWNQDGANMEEQLTAAGYEVDL
                     QYASNDIQTQVSQLETMISNGCQLLVIASIDGDSLGTVLAQAKEANIPVIAYDRLIMN
                     SDAVPYYATFDNYMVGTKQGEYIEDALDLANADGPFNLEIFTGDPGDNNARFFYQGAM
                     DVLQPYIDDGKLVVQSGSIDFEKVATANWATETAQSRMDAIIASYYADGTKLDAVLCS
                     NDSTALGVENALEAAYTGDWPIITGQDCDVANVKNMIAGKQSMSIFKDTRDLAAKTVE
                     MVDAIMKGGEAPVNDTSSYDNGTGIIPTYLCEPVFADVNNYKELLIDSGYYTEADLQ"
     gene            complement(2471..3517)
                     /locus_tag="PLU43_01100"
     CDS             complement(2471..3517)
                     /locus_tag="PLU43_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO27032.1"
                     /translation="MMRKSYLVLLLAAVIFCAGGCKNAEEDNEKTVEVSEDKLEIGMS
                     FDSFIIERWQRDRDVFVSTAKELGAEVNVQTANGEVDEQISQIEYFIDKDVDVIVVVP
                     IDADKLEDVIGKAKKAGIPVMCYDRVARNSNADLFISFDNEEVGALMAAALYGAAGRD
                     ADVVMVCGPETDYNVEMVGEGFDRVTKEYHGNIIGSFHCDAWRAETAYEYVNENMQLF
                     SQADAIMCGNDSLAGEVIRALSEMRLAGKIPVVGQDADLEACQRIVEGTQLMTVYKPV
                     EKLAKKAAEYAVRLAKHEELTDTDTFYDGKYNIPYVKLEPVRVTKDNMDEAVISSGFH
                     LKEDVYMNVSQQNQ"
     gene            complement(3514..5112)
                     /locus_tag="PLU43_01105"
     CDS             complement(3514..5112)
                     /locus_tag="PLU43_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27033.1"
                     /translation="MYKVMLADDEGIVIDSLKFIIEKHFGDVCQVEFAKTGRHVIELA
                     ESYRPDIAIMDIQMPGINGIDAMKEIRKANAATIFIVLSAYDKFDYAKEAINLGVLEY
                     LNKPVEQRRIVEVLKKAMNIINREREKRSNDLIIKEKLETVVPIIENNMIYTILFQDN
                     YEEEIDNFKNLLVITEDYGFFIVLEYGESQEGNHLTNVVGASVRTQSYYKELREIVKE
                     FFVCVIGSLMANRVIVFVPYEKSVLDYNERIEIIEKTRSMIKKLNAHIDVKFRAGIGS
                     TQKLHDCMESYKEAQRALRNTDGTVAHIKDLPVTVSYEEDYPIETEKELFEMTEKGNE
                     NGALVAANHFFDWMVEKYADHITDVKLKALEFVLWAEHLAYEEGGMTYHFLSRQDYLP
                     AILNMQSLDEIKAWFISKILSACRNVVQDKEKQYTSSVEKAKAYMTERYNKDISLDDV
                     SREVDISPYYFSKLFKEETGENFIEYLTGIRIEKAKQLLSGSTLPMKEICAAVGYSDP
                     NYFSRIFKKNVGVTPTEFKEGMTG"
BASE COUNT         1295 a         1375 c          920 g         1584 t
ORIGIN      
        1 gatagcatcc tttacattgt gaaaatgcat ttcttttccg ttgatgtaca gtgttccgct
       61 gatattcgta ccatagctct ttccgaatac actcattgca agttcagtcc gccctgctcc
      121 catcagtccg gatatgccga ccacttcgcc cttatgtaca tttaacgata cgtcatcgac
      181 aactttccgc tcggtatata ccggatgata tacattccag tttttaattt ccatactgat
      241 ctcaccgata tttttttgtt ctctcttagg aaaacgatct gtcagatcac gtccgaccat
      301 gcctttgatg attcgtcctt cggatacgtc gtccgttgcc ttgtccaatg tttcaatcgt
      361 agctccgtca cgaagaatcg tgatcgtatc tgccacatag gaaatctcat ttaatttatg
      421 agatataata atcgaagtca taccatgctc cttaaaagtc agaagcaaat ctaaaagcgc
      481 cctggaatcc tcctcattca gtgaggatgt cggctcatcc agaatcaaaa gcttcgcatt
      541 tttcgcaagg gccttcgcaa tctcaacaag ctgctgttta ccgacgccga tatctttgat
      601 cagtgtcctg gaggactctt caagtcctac tattttaagc agttcgtctg ctcttccata
      661 cgtttccgtc cagtcgatcg ctgccttttt tccccgttca ttgccaagaa acatgttttc
      721 gccgatcgtc atataaggaa tcagggccag ttcctgatga ataataacga ttcccttttc
      781 ttcgctgtct ttgattttga aaaatttaca gacttctccg tcataaataa tatcgccctc
      841 gtaagtacca tatggatagg tgccgctcaa cacattcatc agggtggatt ttccagcacc
      901 gttttctccg accagtgcat ggatctcgcc ttcttcaacc ttaaaattaa cgttgtcaag
      961 cgctttaacc cctggaaaag ttttggtaat atttttcatt tccaataata tttttgccaa
     1021 ttgaattcct cccaatatct aatcagatgg tcacctgtgc atttatacag gcgggttttt
     1081 cacccgcctg cataagcttt attgctttaa attatttact gtaaatctgc ttccgtataa
     1141 tatccggaat cgatcagcag ttctttgtag ttgttaacat cggcaaatac cggctcacaa
     1201 agatacgtag ggataatgcc tgttccattg tcgtaagaag atgtgtcgtt tacaggagct
     1261 tcgccgccct tcatgatagc gtctaccatt tctactgtct tagctgcaag atcacgtgtg
     1321 tctttaaaga tagacattga ctgttttccg gcaatcatgt tctttacgtt agctacgtca
     1381 cagtcctgac ctgtgatgat cggccagtca cctgtataag ctgcttcaag agcattttca
     1441 acaccaagtg cggtagaatc gtttgaacac agaactgcat ccagctttgt tccgtctgca
     1501 taataggaag cgatgatagc atccattctg gactgtgctg tctctgttgc ccagttagct
     1561 gtagcaacct tttcaaaatc gatggaaccg gactgaacta ctaactttcc gtcatcaata
     1621 tacggctgaa gcacgtccat agcgccctga tagaagaatc ttgcattatt gtcgcccggg
     1681 tcacctgtga agatctcaag attgaaagga ccatctgcat ttgccagatc caaagcatct
     1741 tcgatgtact cgccctgttt tgtaccaacc atgtaattat cgaatgtagc gtaataggga
     1801 accgcatccg aattcatgat cagacggtca taagcgataa ccgggatatt tgcttcttta
     1861 gcctgagcca gaactgttcc aagagaatca ccatcgatag atgcgataac aagcagctga
     1921 catccgttag agatcattgt ttcaagctga gaaacctgtg tctggatatc attacttgca
     1981 tactgaagat ctacttcata accggctgct gtcagctgct cttccatgtt ggcaccatcc
     2041 tggttccagc gctgcagatc ctttgtaggc atttccacac cgatcagtcc gccgcttgct
     2101 gcggtctctt cttctgctgc aggtgcttct tcttctgctg ctgccgtgtc atctgcaggt
     2161 gctgcttctt ccgttgcagc tgccgtatca tctgcaggtg ccgcttcttc tgttgctgtg
     2221 cttccacagc ctacaagcaa cgcagctacc attgcagcac taagtactac actaagtaat
     2281 tttttcttca tttcttaaag tcctcccttt aataaaatat aattgacttg taactttgtt
     2341 acaataaaaa tatatcataa atagaatagt acaaatttgt gaacatcttg aattttttat
     2401 gacattccgt tcacaattta aaaagcacta gcattttttt gcgaaaatgc cggtgctttt
     2461 ccaataaatc ctattgattt tgctgagaaa cgttcatata gacatcttcc tttaaatgaa
     2521 acccgctgct gatcacggct tcgtccatat tatcctttgt aacgcgcacc ggttccagct
     2581 tgacatacgg aatattatat tttccatcgt aaaatgtatc cgtatcggta agctcttcat
     2641 gctttgcaag tctcacggca tattctgccg ccttctttgc cagcttctcc accggcttgt
     2701 acactgtcat gagctgtgtg ccctccacga tcctctggca tgcctccaga tccgcatcct
     2761 gtccgacgac cggtatcttt cccgcaagac gcatttccga aagggcgcgg ataacttctc
     2821 ctgcaagact gtcgtttccg cacatgatcg catctgcctg tgaaaacagc tgcatatttt
     2881 cattcacata ttcataagca gtctcagccc gccacgcatc gcagtgaaag ctgcctataa
     2941 tattgccgtg atattctttc gttacccggt cgaatccttc tcctaccatt tccacattgt
     3001 aatcggtctc aggtccgcac accatcacaa catccgcatc acgccccgcc gctccgtaga
     3061 gcgccgctgc catcagggcg ccgacctcct cattgtcaaa ggaaatgaac agatccgcat
     3121 ttgaatttct ggcaacccgg tcatagcaca taaccggaat gccggccttt ttcgcctttc
     3181 caatcacatc ttccagcttg tccgcgtcga tcggaacgac aacgattaca tccacatcct
     3241 tgtcaataaa atattctatc tgggaaatct gctcatccac ttccccgttt gccgtctgca
     3301 cattgacttc ggcaccaagc tccttggccg tggacacgaa tacgtcccgg tctctctgcc
     3361 accgttcaat aataaaggaa tcaaagctca tgccgatctc cagcttgtct tcgcttactt
     3421 ccactgtttt ctcattatct tcctccgcat tcttacagcc gccggcacaa aaaatcacgg
     3481 cagcaagcag cagaaccaga taactctttc tcatcatccc gtcatcccct ccttaaattc
     3541 ggtaggcgtc acaccaacat tctttttaaa aatacggctg aaataattag gatccgaata
     3601 gccgaccgca gcacagatct ctttcatcgg aagcgtggaa ccggaaagca gctgttttgc
     3661 cttttcgata cggatccctg tcagatattc gataaaattc tctcccgttt cctccttgaa
     3721 cagcttactg aaataatatg ggctgatatc tacttctctg gaaacatcat ccaatgaaat
     3781 atccttgtta taacgctccg tcatataagc ctttgctttt tcaacagacg aggtatactg
     3841 cttttcctta tcctgtacaa cattccggca ggcactcagt attttggaaa taaaccatgc
     3901 cttgatctca tcgagcgact gcatattgag gatcgccggc agataatcct gcctggacaa
     3961 aaagtgatag gtcatcccgc cctcttcata ggccagatgc tctgcccaaa gtacaaattc
     4021 caatgctttc agcttgacat cggtaatatg atccgcatac ttttccacca tccagtcaaa
     4081 aaaatgattg gcggcaacaa gtgcaccgtt ctcatttcct ttttcagtca tttcaaacag
     4141 ttccttttcg gtctctatcg gataatcctc ttcataggag acggtaaccg gaagatcctt
     4201 gatatgtgca acagtaccat ccgtattccg aagcgcacgc tgcgcttcct tgtacgattc
     4261 catacaatca tgaagcttct gtgtggaacc gattcctgcg cggaacttta catcaatatg
     4321 ggcattcagc tttttgatca tgctgcgtgt cttctcaata atttctattc tctcattata
     4381 atcaagcaca gacttttcat acggtacaaa aacgatgaca cggtttgcca tcagcgaacc
     4441 gatcacacag acaaaaaact cctttacaat ttcccgcagt tccttatagt atgactgtgt
     4501 gcgtacactt gccccgacaa cgttcgtaag atgattccct tcctggcttt ccccatattc
     4561 cagtacgata aaaaatccat aatcctccgt gatcacaagc agatttttaa aattgtcgat
     4621 ctcctcctca taattatcct gaaacaggat cgtatagatc atattatttt ctatgatcgg
     4681 aacaaccgtc tccagctttt ccttgatgat caggtcattg ctcctttttt cccgctccct
     4741 gttgataatg ttcattgctt ttttcagcac ttcaacgatc cgcctctgct ccaccggctt
     4801 attcagatac tccagcaccc cgagattgat tgcttccttt gcataatcaa acttatcata
     4861 agcagacagc acgataaaga tcgtcgccgc atttgccttt ctgatttctt tcatcgcgtc
     4921 gatcccattg atcccaggca tctgaatatc cataatggca atgtccggac gatagctttc
     4981 cgccagctcg atcacatgcc ggcctgtttt tgcaaactcc acctggcata catctccgaa
     5041 atgtttctca ataatgaatt tcagggaatc gatcacgatc ccttcgtcat ccgcaagcat
     5101 cactttgtac ataataaatc tcctctttaa gtcaagtacc gatttatcag tactttttat
     5161 tttttttgct actt
//
LOCUS       DAOSYE010000034         5169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_10760_length_5169_cov_3.201408, whole genome shotgun sequence.
ACCESSION   DAOSYE010000034 DAOSYE010000000
VERSION     DAOSYE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10760_length_5169_cov_3.201408"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLU43_01110"
     CDS             complement(<1..542)
                     /locus_tag="PLU43_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27034.1"
                     /translation="MDNERKRVYLFLAITFVLTYAIEIGILWPLVGNKSAATTVTEQG
                     IKASMMFIPALSVVITRLITKEGFKNHYMKWTWNRETTKYYIFAWIGPAVLVLCGALL
                     YFLIFPQRFDINMSYLVSVYKENGTAVAAADLRKTIISQIAAAVLIAPLLNCVTCFGE
                     EWGWRGYLLPKLAKKFSLIPL"
     gene            852..1421
                     /locus_tag="PLU43_01115"
     CDS             852..1421
                     /locus_tag="PLU43_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOO27035.1"
                     /translation="MKKKTLAGLIALGVLIVPVLCYTVIVNKNSSGIADEPAFRTSND
                     IANEETVYLDDEAIALADSSGNTTSLRSEALRAYNLVNEQRTAAGLPALTWDSGLETV
                     AAVRAQECSISFSHTRPNGRAWYTVNSAIQGGENLAFGYNDANSALNAWMNSPTHREN
                     ILYPTFTRIAISLYVSDNGTYYWAQEFGY"
     gene            complement(1503..3461)
                     /locus_tag="PLU43_01120"
     CDS             complement(1503..3461)
                     /locus_tag="PLU43_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOO27036.1"
                     /translation="MKGKLLAVLVTAAMLTVLLYGCGQSGETVETGQEDVLEQTEEQN
                     AEDSAAVSAETALDADEMFTDRDYETDYDESECIPIVLSDDGIEAGDGVTVDGTSATI
                     TEEGTYIVSGSLSDGQLIVDTDDTVKVRLILNGVKMSSSDSAPIYVKEADKVFITLAE
                     GTENILSTAGDYAETSEDEDNIDAVIFSKSDLTLNGSGSLSVSTETGNGITSKDDLVF
                     TSGTYTISAGNHGLEGKDSVRVASGTYMITAVQDGIHSNNTEDEAKGFVYIADGEFEI
                     FADDDGIHAERNVTIDCGTITVSDGYEGIEGISIDINGGVINIAVSDDGFNAAGGNDS
                     SGMQGSDFADAGISGGGGGMGGGAPGGGNGGEAPQMPDGESQESSGEESGESMSNADM
                     SADSNTAADNQEGNGGGPGGGMQGDDMQSGDMQSTESSYYIRITGGIINIEAGGDGID
                     SNGALYVSGGETYISGPTNDANGALDYTSTAEITGGIFVAAGASGMAMNFSDSSTQGS
                     LLVTSESSQTGTIALLDEDGNELVSYSPTKEYNSVVISIPEIEEDGTYTVLMGDDTQE
                     ITMDGLLYGSSMGNGMGGGPGGGGQRGGGGQNIGEGTDGNSDMAPSDDSDGNMQPPGD
                     GQGGQGGGPGGQMGPGGGGTPPDQNGNE"
     gene            complement(3480..4169)
                     /locus_tag="PLU43_01125"
     CDS             complement(3480..4169)
                     /locus_tag="PLU43_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOO27037.1"
                     /translation="MLSSLFTGVLESSEETVTAASFLLCVGTSLAIGLFLTFLYSYKS
                     RCSKSFIVTLAILPAVVCMVIMMVNGNLGAGVAVAGTFSLVRFRSAPGSAKEISAIFI
                     AMAAGLAAGMGYLGYAVTFSVIVGVLMAVYSNSNIGNGKSDERQLKITIPENLDYTGV
                     FDDLFEKYTTKCELEGVKTASMGSLFKLTYDITLKNIEEEKLFIDALRCRNGNLEIVM
                     ARKISAAAEEL"
     gene            complement(4162..4935)
                     /locus_tag="PLU43_01130"
     CDS             complement(4162..4935)
                     /locus_tag="PLU43_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOO27038.1"
                     /translation="MEFKNIFERYEIKYLMTRQQQYELYNLMVTHMQPDTFGKSTIYN
                     IYYDTPDRRLIRKSLDGPCYKEKLRVRSYGVAKSESTVFIELKKKYNGIVYKRRMDLN
                     ERLAHNYLEHSMGVGEKSQIGKEIEYFMKFYEQIEPSVFLSYEREAFVGKEDPDFRMT
                     FDKNILMRDYDMDLKLGAYGETLLDDNMILLEIKTACGIPEWLLGFLSENQIYKTSFS
                     KYGCAYQKFLLPRQFCIGAGEENRTTVIEKENGGKKDVA"
BASE COUNT         1393 a         1333 c          869 g         1574 t
ORIGIN      
        1 agcggtatga gactgaattt tttagcaagc ttcggaagca gatatcctct ccatccccat
       61 tcctcaccaa aacacgtaac acaatttaac agcggagcaa tcagaacagc tgccgcaatc
      121 tgactaatta tggtctttct caaatcggcc gccgcaactg cggtaccatt ttccttatat
      181 acggacacca gataactcat attgatatcg aaccgctgag gaaagatcaa aaaataaaga
      241 agcgcgccgc ataacaccaa tacggccgga cctatccagg caaatatata atactttgta
      301 gtttccctgt tccatgtcca cttcatataa tgatttttaa agccttcttt tgtaataaga
      361 cgggtgatga ctacgctaag cgccggtata aacatcatag acgctttgat cccctgctcc
      421 gttacggtcg tagctgcact tttattccct acaagcggcc ataaaatgcc gatctctatt
      481 gcgtaagtaa gtacaaacgt aattgccaaa aataaatata ccctttttct ctcgttatcc
      541 atcgcatatg ctcccttcaa aacaataaac gccatagaca tcaaagtatc tattttattg
      601 acattttccc ctatttatca tatgatttat acaaatcatt gcatttatta tgattataca
      661 ctataaattg catattgcat atcctaaacc agacaattct ttttgttgca tttgatatta
      721 aatgtgatat ttttaaaaaa taattgttgc atttgaggat tgtttgtgac ataatataaa
      781 tgagaaaaag aatacatatt tatatgataa ataaagagat ctcttatcca ggaaaggagg
      841 gtatttatat tatgaaaaag aaaactctcg cagggctgat cgcactcggg gttctcattg
      901 ttccggtttt atgctacacc gtcattgtaa ataaaaacag ttccggcatt gccgatgaac
      961 cggcattccg tacttctaat gatatagcaa acgaagaaac tgtttattta gatgacgaag
     1021 caattgctct tgcagattct tcgggaaata ctacttctct aagaagcgag gccttaaggg
     1081 cttataatct tgtaaacgaa caaagaaccg cagccggtct gccggcttta acatgggatt
     1141 ccggtttgga aacagttgct gccgtacgtg cacaggaatg ttccatatcg ttctcccata
     1201 caagaccaaa tggcagggca tggtatactg taaacagtgc tattcagggc ggtgaaaatc
     1261 tggcttttgg ttataacgac gcaaactccg cattaaatgc ctggatgaac agtcctaccc
     1321 acagggaaaa tattctttac ccgacattca ccagaatcgc catttcttta tatgtttcag
     1381 ataatggcac ttactactgg gcacaggaat tcggttactg attcccatgg atcaaaaaac
     1441 agctcctttt aaaaaggagc tgtttttttg tctgttgtct tgtaaaataa ccgggatcct
     1501 gtttattcat taccgttctg atccggcggt gttcctccac cgcctggtcc catctgaccg
     1561 cccgggccgc ctccttggcc gccctgacca tctccgggcg gctgcatatt gccgtcacta
     1621 tcatccgaag gtgccatatc gctgtttccg tctgttccct ctccaatatt ctggcctccg
     1681 cctcctcttt ggcctccgcc gccaggaccg ccgcccatac cgttacccat actggaacca
     1741 taaagcagac catccattgt aatctcctgt gtatcatcac ccatcaaaac cgtataagtc
     1801 ccgtcttcct ctatttccgg tatactgatc acaacagaat tatactcctt tgtcggtgaa
     1861 taactgacta attcattccc atcttcatcc aaaagtgcga tcgtacctgt ctgtgaagat
     1921 tctgaagtca caagcagtga gccctgcgtt gaagaatccg agaaattcat agccatcccg
     1981 cttgctcctg ccgccacaaa aataccgcct gtaatttccg ccgtactggt gtagtccaaa
     2041 gcaccgttcg catcgttggt cggaccggaa atataagttt caccaccgga cacataaagc
     2101 gctccgtttg aatcgattcc gtctccgccc gcttcaatat ttataatacc gcctgtaatt
     2161 cttatataat aagaggactc tgtgctctgc atatcgccac tctgcatatc atcaccctgc
     2221 ataccaccgc ccgggccgcc tccgtttcct tcctggttat cagctgcggt attactatcc
     2281 gctgacatgt cggcattcga catgctttcg cctgactcct ctccggaaga ttcctgggac
     2341 tcaccatccg gcatctgcgg cgcttcaccg ccatttccac cgccaggtgc tccgcctccc
     2401 attccgcctc cgcctcctga aataccggca tccgcaaaat cactgccctg cattccagag
     2461 ctgtcattgc cgccggctgc attaaaaccg tcatcagaca cggcaatatt gataactccg
     2521 ccattgatgt caatagatat accttcaatt ccttcataac cgtctgaaac ggtaatcgta
     2581 ccacagtcta ttgttacgtt tctttccgca tgaattccgt cgtcatcagc aaaaatttca
     2641 aattcgccgt ctgcaatata tacaaatcct tttgcttcat cctcagtgtt attgctgtgg
     2701 ataccatcct gtaccgcagt gatcatatag gtacctgatg caacacgtac gctgtccttt
     2761 ccttccagac catgatttcc tgcgctgatc gtatatgttc cgctggtaaa tacaagatca
     2821 tcctttgacg tgatcccatt tccggtttcc gtcgatacac tcaggcttcc gctgccattc
     2881 aatgtcagat ccgacttgga aaaaatgact gcatcgatat tatcctcatc ttcggaagtt
     2941 tctgcataat cccctgcagt ggataatata ttttccgttc cttcagccag cgtaataaat
     3001 actttgtccg cttctttcac atagatcggt gctgaatcgg aagaactcat ctttacacca
     3061 tttaaaatca gtctgacctt cactgtatca tccgtatcaa caataagctg gccatccgac
     3121 aggctgccgc tgacaatata cgtaccttct tctgtaattg tcgcactcgt gccgtccact
     3181 gtcacaccat ctcctgcttc aattccatca tcagaaagca caatgggaat acattcactt
     3241 tcatcgtaat cagtttcata atctctgtcc gtaaacattt catctgcatc cagcgcagtt
     3301 tccgccgaca ccgcagcgct gtcctctgca ttttgttcct ctgtctgttc taaaacatct
     3361 tcctgtcctg tttcaactgt ttctccgctt tgtccgcacc catataaaag aacggtaagc
     3421 attgctgcgg ttaccagaac cgcaagtaat tttcctttca tgatttcttc cctccccgcc
     3481 tataattctt ctgccgcagc agatattttc cttgccatga caatttccag attcccattt
     3541 ctgcatctca atgcatctat aaaaagtttt tcttcctcaa tatttttcag cgtaatatca
     3601 taggtaagct taaacagact gcccatactg gctgtcttca caccttccag ttcacatttc
     3661 gtcgtatact tctcaaaaag atcatcaaaa actcccgtat aatctaaatt ctccggaatc
     3721 gtgatcttta actgtctttc atcactctta ccatttccga tattactgtt tgagtaaaca
     3781 gccatcaaca ctccgacaat cacgctgaat gtgaccgcat atcccaaata tcccattcct
     3841 gcggcaagcc cggccgccat tgctataaat atcgcgctga tttctttagc acttcctggc
     3901 gccgaccgaa aacgaacaag actgaatgtc cctgcaacgg caactccggc gccaagattt
     3961 ccgtttacca tcatgatcac catacagaca actgccggta aaatcgcgag tgtcacaata
     4021 aagctctttg agcacctgct tttatatgaa tataaaaatg taagaaacaa cccaattgca
     4081 agcgatgtac ctacacatag cagaaatgag gcggctgtga ccgtttcttc actgctttcc
     4141 aaaactccgg taaataaact gctaagcaac atcctttttc cctccgtttt ctttttctat
     4201 gacagtagtt ctattttctt caccagctcc tatgcaaaac tgccttggca gtaaaaactt
     4261 ctgatatgca catccatatt ttgaaaacga agttttataa atttggtttt cacttaagaa
     4321 accaagaagc cattccggta tcccgcatgc cgttttgatc tcaagaagga tcatgttatc
     4381 atcaagaagt gtttccccat aagccccaag tttcagatcc atgtcgtaat ctctcattaa
     4441 gatgttttta tcaaacgtca tcctgaaatc aggatcctct tttccaacaa atgcctctct
     4501 ctcatatgaa agaaatacgg acggttctat ctgttcataa aacttcataa aatattcgat
     4561 ttcctttccg atctgtgact tctctccaac tcccatggaa tgttccaggt aattgtgtgc
     4621 cagccgttca ttcagatcca tccggcgttt atatacgatt ccgttatatt tcttttttaa
     4681 ctcgataaac actgtgcttt ccgactttgc aacaccgtag cttctgaccc tcagtttttc
     4741 tttatagcac gggccgtcca gtgactttct gatcagccgt ctgtccggcg tatcataata
     4801 aatattatat atggtactct ttccgaatgt atccggctgc atatgtgtca ccataagatt
     4861 gtataattcg tattgctgct gccttgtcat caaatattta atttcatatc gctcaaatat
     4921 atttttaaat tccatagtat tcactcctct cctgatttca aaaaacttac cggtaaagtg
     4981 catttgcttc gtataagttt tattacttta cacagagtat acaaaagtat tctgaaaaaa
     5041 accttaaata tgcaaaaaac ttttttttgt atttttagcc ggattgtgct ttacgaattt
     5101 tacaggctgt gatatgttta tttctagtga ttaaaataga atgaaaggta ttaggggaga
     5161 aaatcatta
//
LOCUS       DAOSYE010000035         5106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_10950_length_5106_cov_2.712334, whole genome shotgun sequence.
ACCESSION   DAOSYE010000035 DAOSYE010000000
VERSION     DAOSYE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_5106_cov_2.712334"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLU43_01135"
     CDS             complement(<1..124)
                     /locus_tag="PLU43_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOO27039.1"
                     /translation="MDLYSGYISKLIAELSILPGIGSKSAQRLAFHIINMPKENV"
     gene            complement(167..520)
                     /locus_tag="PLU43_01140"
     CDS             complement(167..520)
                     /locus_tag="PLU43_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOO27040.1"
                     /translation="MAKRGGFPGGGMPGNMNNLMKQAQRMQRQMEESQKELEEKEFTA
                     AAGGGAVEITVSGKKEITKVKLSEEVVDPDDIEMLEDLIMAAANEAFRKMEEESQNTM
                     SKLTGGLGMGGGLPF"
     gene            complement(554..2137)
                     /gene="dnaX"
                     /locus_tag="PLU43_01145"
     CDS             complement(554..2137)
                     /gene="dnaX"
                     /locus_tag="PLU43_01145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOO27041.1"
                     /translation="MSYTALYRKWRPERFEDVKGQDHIVTTLKNQITADRIGHAYLFC
                     GTRGTGKTSIAKIFAKTVNCEHPVNGSPCGTCASCRSIAAGNSMNVIEIDAASNNGVD
                     NIREIRDEVAYSPAEGKYKVYIIDEVHMLSIGAFNALLKTLEEPPSYVIFILATTEVH
                     KIPVTILSRCQRYDFKRISVDTISARLKELLKEEKVEAEEKAVRYIAKAADGAMRDAL
                     SLLDQCISFYFGQKLTYDHVLEVLGAVDTEVFSTLLRSIMARDILDAIHLLEKIIMQG
                     REMSQFVTDFIWYLRNLLLVKASEDAEDVLDISSENLQHLQEEAEMINQETLMRYIRI
                     FSELSGEIKYASQKRVLVEIAIIRLCKPSMETDLDSMLERIRDLEEQMENNAAAAYGR
                     SERRNDAAAADKEELPIKTEKPELPKALTADIKKLVSMWPGVIAEMPGVIRPYLKMAK
                     LTAREDDKLLIIVQDTLARDFLNKEMQKAAITKVIDERIGSHVEYKILMMEKNEKFQE
                     NYVDLSKVIHMEIEIEDDF"
     gene            complement(2204..3283)
                     /locus_tag="PLU43_01150"
     CDS             complement(2204..3283)
                     /locus_tag="PLU43_01150"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOO27042.1"
                     /translation="MKRVGLLTSGGDCQALNAAMRGVVKALWFALGAQGVEIYGFFNG
                     YRGLIYSNYKKLDFSDFSGILTRGGTILGSSRTPFKLIREPDENGVDKVAAMKQNYYK
                     LNLDALVILGGNGTHKTANLLSEEGLNIVTLPKTIDNDLWGTDMTFGFQSAVNLATNV
                     IDCIHTTASSHSRVFIVEIMGHKVGWLTLNAGIAGGADIILIPEIPYDIDKIVEAIDL
                     RTKRGSTFTILAVAEGAISKEDAELPKKELKEKLAKRTYPSVSYEIAAQIEERTGQEI
                     RVTVPGHMQRGGEPCAYDRVLASRLGTKAAEMIMKEDYGYMVGIINREVAEIPLKEVA
                     GKLKGVSPDATIVKEAKMLGISFGD"
     gene            complement(3352..4275)
                     /locus_tag="PLU43_01155"
     CDS             complement(3352..4275)
                     /locus_tag="PLU43_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27043.1"
                     /translation="MKYRKTIITILTALVVFFGIVMLKNCCSCLIPNHVMGVRSIIAQ
                     KQVDTLFLGSSAYRKGLDMYMLEEELDGESYALTYNGNQPLNMALELKEILNAGTKIK
                     YLIVDFNPSMMDRGADLSDKRLLWDISMEGKLSLWKELAKKEDTDFFTFYDYFVLSNN
                     DYMITYPFSYPLIAKRYYRGGSTQADEASGSTQEELEALEVVEDPGIDELQLQSVGEI
                     ITRCMENDIRLIFMESPRYQTMADNQNYADKSAALKAYLEEQDVETVLTADLGFDNTN
                     PAYYSDLTHMSGEGKREFTALVAEYLKQREK"
     gene            complement(4288..>5106)
                     /locus_tag="PLU43_01160"
     CDS             complement(4288..>5106)
                     /locus_tag="PLU43_01160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO27044.1"
                     /translation="LLCATVFYAVQIYCDFSGYMDIVIGVSELFGIKIAENFNKPYFS
                     KSIAEYWRRWHITLGTWFKDYLYYSVIRSEFCRRIGKKFGKGRLKDIGRTVPTVIGLL
                     VVWFMTGLWHGASWHYVAHGLYHGGIIILSVLLAPVYKKVRIRMHIEDASRPYHVLQM
                     LRTFILVDISYILFRSATMEDAGYIMNRIFFHLQISFGALKEAVLPFTEDNTAVAYFG
                     VVAAAMALLFISEVLDYNHKQILKKHRYINAALMILAVLLFGVFGQSGFIYAAY"
BASE COUNT         1278 a         1387 c          914 g         1527 t
ORIGIN      
        1 caacattttc tttcggcata ttgatgatat gaaaagcgag ccgctgtgcc gattttgaac
       61 cgatccccgg taaaatcgac agctccgcta ttaatttact gatatatcca ctgtatagat
      121 ccattctgcc atctccggat tctcaaaata actgcctgct ggatcattag aatggaaggc
      181 ctccgcccat accaagaccg cctgtcagct tcgacatcgt gttctgggac tcttcttcca
      241 tcttacggaa tgcttcatta gccgcagcca tgatcagatc ttccagcatt tcgatgtcat
      301 caggatccac tacttcttca gacagcttta cctttgtgat ttcctttttc cctgaaacgg
      361 taatctctac cgccccgcct ccggctgccg ccgtaaattc cttttcttcc agctccttct
      421 gactttcctc catctgacgc tgcatccttt gcgcctgctt catcagattg ttcatattac
      481 caggcattcc tccgcctgga aaaccacctc tttttgccat ctcttttcct cctgaagtat
      541 ctgtttataa aaattaaaag tcatcctcta tctcgatttc catatgaatg actttgctta
      601 aatccacata gttttcctga aatttttcgt ttttttccat cataaggatc ttatactcca
      661 catgactgcc aattctctcg tcgatcacct ttgtgattgc tgctttctgc atttccttat
      721 tcagaaaatc ccgcgcaagc gtatcctgca caatgatcag cagcttgtcg tcctctctgg
      781 ccgtcagttt cgccatcttt aaatacggtc tgatcacacc cggcatttcc gcaatgaccc
      841 cgggccacat cgatacaagc tttttaatgt ctgccgttaa agcttttgga agctccggct
      901 tttcagtctt tatcggcagt tcctctttat ctgcagcagc cgcatcattc cttctttcgc
      961 tcctgccata agcagcggcc gcattgtttt ccatctgttc ctccagatct ctgatcctct
     1021 caagcattga atccaaatcc gtttccatgc tcggtttaca aagcctgatt atagcgattt
     1081 ccaccagtac ccgtttttga gaagcatatt tgatctcacc ggacagctca gaaaaaatac
     1141 gaatatacct catcagcgtt tcctgattga tcatctctgc ttcttcctgt aaatgctgca
     1201 gattttcgct tgatatatcc agtacatcct ctgcatcttc cgaagccttt acaagcagaa
     1261 ggtttcgcag ataccatata aaatctgtca cgaactggga catctccctg ccctgcatga
     1321 ttatcttttc cagcaaatgg atcgcgtcca aaatatccct tgccataatg gaccgcagca
     1381 gcgtactaaa tacttccgta tcgactgctc cgagaacctc cagtacatga tcatatgtca
     1441 gcttctgccc gaaataaaac gaaatacact gatccaaaag acttaaagcg tcgcgcatcg
     1501 cgccgtctgc tgcttttgca atataacgga cagctttttc ctccgcctcg accttttctt
     1561 ctttcagcag ttctttcagc cgggctgaga tcgtatccac ggaaattctc ttaaaatcat
     1621 accgctggca cctggacagg atcgtcacag gtattttatg cacttccgta gtcgccagaa
     1681 taaaaataac ataggacggc ggctcctcaa gcgtctttag caatgcatta aaggcgccga
     1741 tcgaaagcat atggacctca tcaataatat agaccttata ttttccctct gccggtgaat
     1801 aagcaacttc atccctgatt tcacggatat tatccacccc attattagac gctgcatcga
     1861 tctctattac attcatggaa ttgccagccg caatcgacct gcagctggca catgttccgc
     1921 aaggacttcc gtttaccgga tgctcacagt ttaccgtttt ggcaaatatt tttgctattg
     1981 atgtttttcc tgttccccgc gttccgcaga acagatacgc atgtccgatt ctatccgcag
     2041 taatctgatt tttcaatgtc gtaacaatat gatcctgtcc ttttacatct tcaaatcgtt
     2101 cgggtctcca ttttctgtac aatgcggtat atgacatgtg cgttcctctt tctcatacga
     2161 aaccattaca aaccacgtaa tgatataact gccggattcc ggcttaatcg ccaaaactga
     2221 taccaagcat tttggcttcc tttactatcg tagcatccgg cgaaacgccc ttcagcttgc
     2281 cggccacttc ttttaatgga atttcggcaa cttcacggtt gatgatccct accatatatc
     2341 catagtcctc tttcatgatc atttccgccg cctttgttcc aagcctggac gccagtactc
     2401 tgtcatacgc acacggctca ccgccccgct gcatatgtcc cggcacggtt actctgatct
     2461 cctgtccggt tcgctcttca atttgagcgg caatttcata agaaacagac ggatatgttc
     2521 tttttgccag tttttctttc agttcttttt tgggcagctc tgcatcttct tttgagatcg
     2581 caccttctgc cactgcgaga atcgtaaagg tgctgcccct ctttgtcctt aaatcgatcg
     2641 cttcaacgat cttatcgata tcatatggga tctccggtat caaaataata tctgcaccgc
     2701 ctgcaatacc ggcattcagc gtcagccagc cgaccttgtg tcccatgatc tccacgataa
     2761 ataccctgct gtgggaggat gctgtcgtat ggatacaatc gatcacattc gtagcaagat
     2821 ttactgcgct ctggaatcca aacgtcatat ccgttcccca taaatcatta tctattgtct
     2881 ttggaagcgt aacaatgttc aaaccttctt cacttaacag atttgccgtc ttatgcgtgc
     2941 cgttgccccc aaggatcaca agtgcatcca ggttcagctt gtaataattc tgtttcattg
     3001 ccgcaacttt atccacgccg ttttcatccg gttcacgaat cagtttaaac ggggttcttg
     3061 aactgccgag aattgtgccg ccgcgcgtca agataccaga gaaatccgaa aaatccagct
     3121 ttttataatt gctgtagatc agccccctat aaccgttgaa aaatccataa atctcaacac
     3181 cctgcgctcc aagcgcaaac cataatgctt ttacgacacc tctcattgct gcattcaatg
     3241 cctgacagtc accgccgctt gtcaataacc caactcgttt cattatgaca cttcctttct
     3301 ttttgttctt gatttccttt taagtatacc ccatttccat ctctttttct actacttctc
     3361 gcgctgtttg agatattccg caaccaatgc ggtaaactcg cgtttacctt caccggacat
     3421 atgtgtcaga tcggaataat aggccggatt tgtattgtca aagcccagat ctgccgtcag
     3481 tacggtctca acgtcctgtt cttccagata tgcctttaac gctgcagact tatccgcata
     3541 attttgattg tctgccattg tttgatatcg cggcgactcc ataaagatca gccgaatatc
     3601 attttccatg cagcgtgtta tgatctctcc tacggactgc agctgcaatt catcgatccc
     3661 cggatcttcc acgacctcca gtgcttccag ctcttcctgt gtgctccccg atgcttcgtc
     3721 cgcctgtgtg ctcccgcccc tgtaatacct ttttgcgatc agaggatagg agaacggata
     3781 ggtgatcata tagtcattat tggaaagtac aaaataatca tagaatgtaa aaaaatccgt
     3841 gtcttccttt ttagcaagct ccttccataa agaaagcttt ccttccatac tgatatccca
     3901 aagcagccgt ttatcgctca gatcggcgcc tctgtccatc atgctcggat taaaatccac
     3961 gatcagatat tttatttttg taccggcatt gagtatttct ttaagctcca gagccatatt
     4021 cagcggttgg tttccattat atgtcagcgc ataggattct ccgtcaagct cttcttccag
     4081 catatacata tcaagacctt ttcgataagc ggaagaacca agaaacaaag tatccacctg
     4141 tttttgggca atgatcgaac ggactcccat cacatgattg ggaatcaggc aggagcagca
     4201 gttcttcagc atgacaatac cgaagaatac aaccagtgct gttaagatcg taatgatagt
     4261 ttttctgtat ttcatcccgt aattcctcta atatgccgcg taaataaagc cggactgtcc
     4321 aaatactcca aacagcagca ctgcaaggat catcagcgcg gcatttatat accggtgttt
     4381 tttcagtatc tgtttatgat tgtaatccag cacttccgaa ataaacagca gagccattgc
     4441 agccgcaaca actccgaaat aggctacggc cgtattgtct tcggtaaacg gcagaactgc
     4501 ctccttcaat gcaccaaaac tgatctgcag atggaaaaag atccgattca taatatagcc
     4561 tgcgtcttcc attgtcgcgg accggaacaa aatataagaa atatctacaa gaatgaacgt
     4621 acgaagcatc tgaagcacat gatacggcct ggatgcgtct tcaatatgca tccggatccg
     4681 cactttttta tacacgggtg caagcagcac gcttaaaatg ataatgcctc catgatataa
     4741 tccatgcgcc acgtagtgcc agcttgcacc atgccacagg ccggtcataa accagacaac
     4801 aagcagtccg atcactgttg gtaccgttct gccgatgtcc tttagtctgc cttttccgaa
     4861 tttctttccg atccgtctgc agaattcgga ccggatcacg gaataatata agtaatcctt
     4921 aaaccatgtc cccagcgtaa tatgccatct tcgccaatat tccgctattg acttcgaaaa
     4981 atagggctta ttgaaatttt cagcaatttt tatcccaaac aattcgctga caccgatcac
     5041 gatatccata tatccggaaa agtcgcaata gatctgaacg gcataaaaca cggtcgcgca
     5101 aagcag
//
LOCUS       DAOSYE010000036         5092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_10987_length_5092_cov_3.497717, whole genome shotgun sequence.
ACCESSION   DAOSYE010000036 DAOSYE010000000
VERSION     DAOSYE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10987_length_5092_cov_3.497717"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLU43_01165"
     CDS             <1..885
                     /locus_tag="PLU43_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27045.1"
                     /translation="MALTAKKAYSINMGPFPAGIYRTGYPYLYRAPKGYTQEEAVQYY
                     IDSLYRLFDEATPAGDVACIIMEPLQGEGGFIPAPISYVKALRKICDENGILLIADEV
                     QTGFCRTGKLFASEYWKEAGILPDIMTMAKSLGGGMPISAITGRNEIMDAALPGTIGG
                     TYCGNPLACASALQAIDIYEKEDLAGRSMEIGDKVRTAYESFQKTYPVIGDIRGLGGM
                     LGIEFVKDPETKEPYPEFVSRMIQNALSRGLLIENAGTYGNVIRFLAPLVITDEQLDA
                     GLAIFEECLLETTKQLVK"
     gene            902..2032
                     /locus_tag="PLU43_01170"
     CDS             902..2032
                     /locus_tag="PLU43_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO27046.1"
                     /translation="MEFMYHLPVNLIFGAGKVKETGKIAAGFGKKVFLVTGRNSTKKS
                     GLLDRVKGSLEKEGLSVLVFDQVTQNPLTTTALEGASIAKTNGCDIVVGLGGGSIMDC
                     AKAIAFLAVNDGDINDYIFNRKSSTTALPIVLIPTTCGTGSEGNGFAVLTNPDNNDKK
                     SLRCNAIIAKASIIDPECMLTMPKGVTASVGFDAFCHCMEAYLSKITNPIAETMCING
                     MQMISEHLVRAYESSQDLEAFEALSLASTYGGMVIHMAGVTLPHGMEHPASGLKDIVH
                     GKGLAALTPVISEASYEAAPDKYETVSKILGGKSGADCGDRIRMLLESIDLQIGLSDL
                     GIEEGDIPWMAENCMKVSAAGISNHPRVFDQKEIEELYRKAL"
     gene            2276..>5092
                     /locus_tag="PLU43_01175"
     CDS             2276..>5092
                     /locus_tag="PLU43_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO27047.1"
                     /translation="MEKNSKSLWLSIIIIYFFIYMMVISFQLDLAAAVLRPLSSFFVL
                     WSLKKFVSLHIVHERTKRTVCLAFLFKFAADLAVLMNEIPILHLDEYRNFITIVRMGL
                     CALFLLHLLVGNFEFYYYLTKKLNRFQVIADVVMIAACVSTEIWLVFIKGNRALLANS
                     ILTFTAQESVEIIFLFIYAVMSLFLLGTLLIVWFYFQKVRMTLGQRLIMLGTACIAFL
                     YLLSAIWPGLFSGSVLGSFFLQTAILLFAIGCIFYDDYPHRFFSGGKEEQTTNSGTWK
                     NAFYLVVYPIFSILASGFKFTVLLFLLYITFYIICCLYVKQISVTSSLLEREKEYNRQ
                     LKVYSSVLEQAPLSVIITDTEGAMEYVNPYFTKVSGYSFQEALGKNPRILKSTKTSAD
                     TYEVLWDCLKNDRKWIGTLVNMDKDGREFEEKAVMLPIKNEFGAVTNYVAIKEDVTEE
                     NKIKRQLNNQNYFTTQLLDTIPSTIFYVSAKDIILGENKECKRIFGIRDDRYVGTKLQ
                     NAPWMDEQKYRHFVQMKKEALENDKAGIKRIWRTLPDGKTLTLLFSVNPFYLSDGQLG
                     GFLGVMTDVSELAKKERELEEALKQANAATEAKSQFLANMSHEIRTPMNAVIGMSYLT
                     LKTDLNTQQRDYVNKIYTAANSLLGVINDILDFSKIESGMLELEETEFDLDRVVANSV
                     DLLVQKANEKQLEFLYHLPPDIPQYLRGDSLRLGQIITNLVSNAVKFTKQGEIEIDVE
                     RVERREDRICIKFSVRDTGIGIPNEKLESLFEPFTQSDSSTTRQFGGTGLGLTICRKL
                     VGMMGGRIWVESDYGKGSTFFFTAWFLIGEEAKLRETPPAYAVHGRRVLIVDDNHNAR
                     EIMSEYLRTMGFLTETADSGEDALHMLFEEDKQESFDVVFMDWKMPGLDGIQTIRKMK
                     ETQTLKDAPSVILITAYDMNEMMKQ"
BASE COUNT         1448 a          949 c         1315 g         1380 t
ORIGIN      
        1 atggcattaa cagcgaaaaa ggcgtattct ataaacatgg gtcctttccc ggcgggcatt
       61 tacagaaccg gatatccgta tttatatcgt gcaccgaaag gatatacaca ggaggaagct
      121 gttcagtatt atatcgacag tctgtaccga ctgtttgatg aagcgacgcc ggcaggggat
      181 gtagcctgca tcatcatgga accgcttcag ggagaaggag gctttatccc tgcaccgatc
      241 agttatgtaa aagcgcttcg caaaatatgt gatgaaaacg ggatcctgct gatcgcagac
      301 gaagtacaga ccgggttctg tcgaaccgga aaactgtttg cgtcagaata ctggaaagaa
      361 gcagggatcc ttccggatat catgacaatg gcaaaatctc ttggaggcgg tatgccgatc
      421 agtgcgatca caggcagaaa tgagatcatg gatgcagcac ttccgggaac aataggagga
      481 acctattgcg ggaatccgct tgcctgtgca tcggctttgc aggcgatcga tatttatgaa
      541 aaagaagacc tggcagggcg atccatggaa atcggagata aagtacgcac tgcatatgaa
      601 agcttccaga aaacctatcc cgtcattgga gatatcagag gacttggcgg aatgcttggc
      661 atagaatttg taaaggatcc ggaaacaaag gagccgtacc cggaatttgt aagccggatg
      721 attcaaaatg cgttaagcag gggactgctg atcgaaaacg cgggtacgta tggaaatgtc
      781 atccgttttc tggcaccgct tgttattacg gatgaacagc tggatgcagg cctggctatc
      841 tttgaggaat gtcttctcga aacgacaaaa cagcttgtaa aataatgaat ggagggtttt
      901 tatggaattt atgtatcatt tgcctgtgaa tctgattttc ggagcaggaa aagtaaaaga
      961 aacgggaaag atcgctgccg gtttcggcaa aaaggtgttt cttgtaaccg gaaggaacag
     1021 cacgaaaaaa tccggacttc ttgacagagt gaaaggttct ttggaaaaag aaggtctttc
     1081 tgttcttgta ttcgatcagg tcacgcagaa ccctcttaca acaacagcac tggaaggtgc
     1141 gtccattgca aagacaaacg gctgtgatat cgtagtgggt cttggcggag gttctattat
     1201 ggactgtgca aaggcgatcg cttttctggc ggtcaatgac ggtgatatca atgattatat
     1261 ttttaacaga aagagcagca cgacggcgct gccgatcgta ctgatcccta cgacgtgcgg
     1321 aacgggcagc gaaggaaacg gatttgcggt tctgacaaat ccggataaca atgataaaaa
     1381 atcactgcgc tgcaatgcga tcattgcgaa agcttccatt attgatccgg agtgcatgct
     1441 taccatgcca aagggcgtaa cggcttcagt tggttttgat gcgttctgcc actgcatgga
     1501 agcctatttg tcaaagatca cgaatccgat cgcggaaacg atgtgtatca acggtatgca
     1561 gatgatctcg gagcacttgg tccgtgcgta tgaaagcagt caggatctgg aggcgtttga
     1621 ggcactgagc ctggcaagta cttacggcgg aatggtgatc catatggcag gggtaacgct
     1681 gcctcatggg atggagcatc cggccagcgg tctgaaagat atcgtacacg gaaagggact
     1741 tgcagcactg acaccggtca tcagcgaggc ttcttatgaa gcagcgccgg ataaatatga
     1801 gaccgtcagt aagatcctcg gcgggaaaag cggagcagac tgcggagatc gtattcgcat
     1861 gctgttagaa agcatagacc tgcagatcgg tctgtccgat ctcggtattg aagaagggga
     1921 tatcccgtgg atggcagaaa actgcatgaa ggtgtctgct gccggaatat ccaatcatcc
     1981 gcgtgttttt gaccaaaaag agatcgaaga gctgtacagg aaagcactgt aaacgaataa
     2041 aaacttttat atattaagaa aatggagaga acggcatccg gctgcggagg ctgttctctt
     2101 tttgcctttt agaacgaaaa ataaaacgga aatccttagt gtacatccgt atcttttgtg
     2161 gtatagtaaa tataaagtgg tatttttacc caattagtat tagatatgat actatttata
     2221 tcaatctgct gccggttgat atattattag agcatacata tgggaggaca gcaaattgga
     2281 gaaaaattca aaatcattgt ggctgagcat aatcattatt tacttcttta tttatatgat
     2341 ggttatttca tttcagcttg atctggctgc tgccgtatta agaccgctat catctttttt
     2401 tgtgctttgg tctctgaaaa aatttgtttc tctgcatatt gttcatgagc gtacaaaaag
     2461 aacggtctgt cttgcgtttc tgtttaagtt tgccgcagat ctggcagtac tgatgaatga
     2521 aataccgatc ctgcatctgg atgagtacag aaattttatt accattgtca ggatggggtt
     2581 gtgtgccctt tttcttctgc atctgcttgt cggtaatttt gaattttatt attatctgac
     2641 aaaaaaactg aaccggttcc aggtcatagc ggatgtcgtt atgatcgcag cctgtgtttc
     2701 tacggagatc tggctcgttt ttataaaagg aaacagagcg ctgctggcaa atagtattct
     2761 gacttttacg gctcaggaaa gtgtggaaat tatttttttg tttatctatg ccgtgatgtc
     2821 actatttttg ctcggaacgc tgctgatcgt ttggttttat tttcaaaaag tcaggatgac
     2881 cctgggacag agactgatta tgcttggcac tgcatgcatt gcatttcttt atctgctgtc
     2941 tgctatttgg cccggtttgt tcagcggatc tgttttaggt tcattttttc tgcagaccgc
     3001 gattcttctt tttgcaatcg gctgtatttt ttacgatgat tatccacacc ggttcttttc
     3061 aggcggaaaa gaggagcaga caacgaattc cggtacatgg aaaaatgcgt tttatcttgt
     3121 tgtttatccg atattctcca ttttggcttc cggatttaaa tttacggtac ttttgtttct
     3181 gctttatatc acgttttata ttatctgctg tctctatgta aagcagattt ccgtgacaag
     3241 cagtctgctg gaacgggaaa aggaatataa cagacagctg aaggtatatt ccagtgttct
     3301 tgaacaggca ccgctttcgg tcatcattac ggatacggaa ggagctatgg agtatgtaaa
     3361 cccttatttc acaaaagtat ccggttattc tttccaggag gcgcttggta aaaatccgag
     3421 gatcttaaaa tccacaaaaa catcggcaga tacatatgag gttttgtggg actgcctgaa
     3481 aaacgaccgt aaatggatag ggacgcttgt taatatggat aaagacggca gggagtttga
     3541 ggaaaaagcg gtcatgcttc ctatcaaaaa tgaatttggc gcagtgacta attatgtggc
     3601 gatcaaggaa gatgttactg aagaaaacaa aatcaaaagg cagcttaata atcagaacta
     3661 ttttaccaca cagctgctgg atacgatacc gagtacgatt ttttatgttt cggccaaaga
     3721 tattattttg ggagagaata aggaatgcaa acggattttt ggaattcggg acgacagata
     3781 tgtcggaaca aagctgcaga atgcaccatg gatggatgag cagaaataca ggcattttgt
     3841 tcaaatgaaa aaagaggcac tggaaaatga taaagcaggt atcaaacgta tctggcgaac
     3901 gctgccggat ggaaaaacgc ttacacttct gttcagtgtc aatccattct atttgtccga
     3961 cgggcagctg ggtggatttt taggtgttat gacggatgtt tcagagcttg caaagaagga
     4021 aagggaattg gaagaagcgc tgaaacaggc aaatgccgca accgaggcca aatcgcagtt
     4081 tctcgcaaat atgagccatg agatccggac gccgatgaat gcggttatcg gcatgtcata
     4141 tctgacgctg aagacagacc tgaacacgca gcagcgggat tatgtcaata aaatttatac
     4201 agccgcaaat tcgctgcttg gagtcattaa tgatattctt gatttttcaa agatcgagtc
     4261 cggtatgctg gagctggaag aaacggaatt tgatctggac agggtcgtag cgaattctgt
     4321 tgatcttctt gtccagaaag caaatgaaaa acaactggaa tttttatatc atcttccgcc
     4381 tgatatcccg cagtatttga gaggggattc gttaaggctc ggccagatca ttacaaatct
     4441 tgtgagcaat gcggtgaaat ttacgaaaca gggagagatc gagatcgatg tagagcgtgt
     4501 ggaacgacgg gaagaccgga tctgtattaa attttcggtc cgtgatacgg ggattggaat
     4561 tccaaatgaa aagctggaga gcctgtttga accgtttaca cagtcagaca gttcaactac
     4621 aaggcagttt ggggggaccg gtcttggact tacgatctgc aggaaactcg ttggcatgat
     4681 gggcggacgt atttgggtgg aaagcgatta tggaaaagga agtacgtttt tctttaccgc
     4741 atggttcctg atcggggagg aagcaaagct gcgggagacg ccgccggcat atgctgttca
     4801 cggcaggaga gtcctgatcg tggatgataa tcataatgcg cgtgagatca tgtccgaata
     4861 tctgcggacg atgggttttc ttacagaaac ggcagattcc ggagaagatg cgctgcatat
     4921 gctgtttgaa gaggataaac aggagtcatt tgatgttgtt ttcatggatt ggaaaatgcc
     4981 tggtctggac ggcattcaga cgatccggaa gatgaaggaa acgcagacgt taaaagatgc
     5041 gccttcggtt atcctgatca cggcatatga tatgaatgaa atgatgaagc ag
//
LOCUS       DAOSYE010000037         5052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_11121_length_5052_cov_3.170903, whole genome shotgun sequence.
ACCESSION   DAOSYE010000037 DAOSYE010000000
VERSION     DAOSYE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11121_length_5052_cov_3.170903"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..75)
                     /locus_tag="PLU43_01180"
     CDS             complement(<1..75)
                     /locus_tag="PLU43_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOO27048.1"
                     /translation="MEKFLFTSESVTEGHPDKLCDAISD"
     regulatory      complement(200..295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            570..1634
                     /locus_tag="PLU43_01185"
     CDS             570..1634
                     /locus_tag="PLU43_01185"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO27049.1"
                     /translation="MKTAEDVYSYNNQMILPRDTVLTDKMITRLEFYSVLAIRITDKN
                     PADTDNEEESGEVWDSSENLSYSQKVKASKQFKEFEKSFLHSTDIFKNKLHSIVEKNA
                     EIDTDQLIHTVTDLISSNMTGISVFDMLHNMRQYDDFTYMHCINVSLICNIFAKWLKL
                     PESDVKTLTLCGLLHDVGKLMIPDNIIRKPSRLSPLEYNIIKTHTLQGYNILKEYPID
                     ENIKESALMHHERCDGSGYPLGLSGDKINPYAKIVAIADVYDAMTAARIYRGPLCPFK
                     VISIFETEGLQKYDSKYILTFLEHIAATYLNNRVRLSNGREGDVVFMNKNCLSKPMIQ
                     CGNDFIDLSRETNLYIETFV"
     gene            complement(1645..2937)
                     /locus_tag="PLU43_01190"
     CDS             complement(1645..2937)
                     /locus_tag="PLU43_01190"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO27050.1"
                     /translation="MEQYIIKGGIPLVGEVEIGGAKNAALAILAASVMSNETVIIENV
                     PDVRDTNVLLQAMESIGVFVEHLDRHTVKVNASAIHNRTIDAGYIKKIRASYYMLGAL
                     LGKYNYAQVALPGGCNIGSRPIDQHIKGFKALGADVKISHGLITAETTRLMGSHIYLD
                     VVTVGATINIMMAASLADGKAIIENAAKEPHVVDVANFLNSMGANIKGAGTDVIRIKG
                     VERLHSTTYSIIPDQIEAGTFMFAAAITKGDVMIRNIIPKHLESISAKLIEIGCEIEE
                     FDDALRIVAARRLTHTHVKTLPYPGFPTDMQPQIAVTLALSDGTSIVTESIFENRFKY
                     VDELARMGANIKVEGNTAIIEGMNRYTGASITAPDLRAGAALVLAGLAADGITLVDDI
                     VYIERGYEDFPQKLTALGAQIERVTAERDIQKFKLKVG"
     gene            complement(3084..4661)
                     /locus_tag="PLU43_01195"
     CDS             complement(3084..4661)
                     /locus_tag="PLU43_01195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO27051.1"
                     /translation="MRRLTKYTKIIKYTHIGAMLFTALLVFLLVPAIGKYSESGNNLI
                     TVFVNGTQVGVVNDSSKVDSMILEARKRIAKENDGLVLINYEVVLSGSRVIFGKTDAK
                     EDVINNIYQVFLRSIMKTKQPVYEVKINEFTVNLKSSNEVLTLLRTAKSKYDENNEFT
                     VDLVLDSSRQLNVLTTKVTKTDEIEEQGEDETSVFPTAGAYQKIQEYYDEALNQETGA
                     FEFGVTNLDFGENVEVVQAYVDSDKISTLDEAIELVTKDQEKSKIYEVEAGDSLSVIA
                     QKNDTTIDEIIAMNKEIIPDENATIRVGDEIKVNMPEPELSVVRTEQVYYEEEYDEDI
                     QYVDNDSWYTTKSQVLQEPVAGFRKVVADITYRNDEEESRELIYEDVVAQAVAKIVER
                     GTKTPPTYMKPVSGGRLSSGFGKRTAPTKGASTYHEGVDWAVPIGTAVVAASGGQVIR
                     AGWGSGYGYCVYIQHPDGIVTRYGHLSKILVKTGQTVKQGEKIALSGNTGVSSGPHLH
                     FEIRVNGTPVNPLNYLN"
     gene            complement(4894..>5052)
                     /locus_tag="PLU43_01200"
     CDS             complement(4894..>5052)
                     /locus_tag="PLU43_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO27052.1"
                     /translation="FLAQVVAVHVDESYMEGARFNLQAADPIVYSHGEYYGLGRLLGS
                     FGYSVRKK"
BASE COUNT         1330 a         1262 c          936 g         1524 t
ORIGIN      
        1 gtccgaaatc gcatcgcaca gtttatccgg atgtccttcc gttacggatt cggatgtaaa
       61 taaaaatttc tccattccaa attcctcctt aaatcaatta atgaaagtgt tcatcacagg
      121 accgtccttt ataaaaattt tcctgtgata taaaaaaaat ccgcttgctt aagcggacct
      181 gataaatgat aatcaaatcc tcttattgtt cgatgactcg ctcaggttgg caccttcctt
      241 ccggggttgc cgtggcttca tagatcctat gtatctccac cactctgaat aagagagaaa
      301 tataatacaa tatggaattt tcttatctgt tttttatcat actctttctc tttctttccg
      361 tcaagatagt tattgggaat tcttttcata aaccttttgg aatcccctga tattattgtc
      421 atatgcccgc atttgtattg acattttcca tattaatttt tatactataa acataaagca
      481 ctctattttt tacaagagct ttacattacc ttgaagggat tgtcggtgaa caaatgaaaa
      541 gagtccgaac ggctgatttg catcctggca tgaaaacggc agaagatgtt tactcttata
      601 ataaccaaat gatactcccc cgtgataccg tcctgacaga taagatgatt acacggcttg
      661 aattctactc cgttcttgcc atcagaatca ctgacaaaaa tcccgcagat acagataatg
      721 aagaagaaag cggcgaagta tgggattcct ctgaaaatct ttcttactcg caaaaggtaa
      781 aagccagcaa gcaattcaaa gagtttgaaa aatccttttt gcacagcacg gatattttta
      841 agaacaagct gcattccatc gtggaaaaaa atgcggaaat cgatacggac cagctgatcc
      901 atacggtcac agacctgatc agcagcaata tgacaggaat ctccgtattc gatatgctcc
      961 acaatatgcg gcaatacgac gattttactt acatgcattg tatcaacgtt tctttgatct
     1021 gcaatatatt tgccaaatgg ctcaaactgc cggaaagtga cgtcaaaaca ctgacacttt
     1081 gcgggcttct gcatgatgtc ggtaagctga tgataccgga caatattatc agaaagcctt
     1141 ccaggctgtc tcctttagaa tacaatatca tcaaaacgca tacgctgcag ggatataaca
     1201 tcctgaaaga atatccgatc gacgaaaaca ttaaagaaag cgccctgatg catcatgagc
     1261 gctgtgatgg ttccggttat cctcttggtc tgtccggaga taagatcaat ccttatgcta
     1321 aaattgttgc gattgccgac gtttacgatg ccatgaccgc agcccgtatt taccggggac
     1381 cgctatgtcc gtttaaagtc atcagcatct ttgaaacaga aggtcttcag aaatatgaca
     1441 gcaaatatat tcttaccttc ctcgaacata tcgcggcaac ttatctgaac aaccgtgtac
     1501 gtttaagcaa cggcagggaa ggtgacgtcg tattcatgaa taagaactgt ctgtcaaaac
     1561 cgatgattca atgcggaaat gattttatcg atctgtccag ggaaacgaat ctttatatcg
     1621 aaacctttgt ataggttttg tcatttaccc tactttcaat ttgaatttct gaatgtcacg
     1681 ctcagccgtg actctttcta tttgtgctcc taaagcagta agcttctgcg ggaaatcctc
     1741 atatcctctt tcaatatata cgatgtcatc cacaagcgtg atcccgtccg ccgcaagtcc
     1801 tgcaagcacc agtgcagctc ccgcacgcag atccggtgcc gtaatggagg ctcccgtata
     1861 tctgttcatg ccttcgataa ttgcagtatt cccttccacc ttaatgttgg cacccatccg
     1921 tgcaagctcg tccacatatt taaaacggtt ctcaaaaata ctttccgtta cgatactggt
     1981 tccgtcggat aatgccagtg ttaccgcgat ctgcggctgc atatcggttg gaaatcccgg
     2041 atacggcaat gtcttaacat gcgtatgtgt aagccgtctt gctgccacga tccgcagcgc
     2101 atcgtcaaat tcctctatct cgcagccgat ctcaatcagt tttgcgctga tcgattccaa
     2161 gtgctttgga ataatatttc tgatcatcac atcgcctttt gtaatcgccg ctgcaaacat
     2221 gaatgtaccc gcttctatct gatccggaat aatggaatac gtcgtactgt gcagtctttc
     2281 cactcccttg atcctgataa catccgtacc ggcaccttta atattggcac ccatactgtt
     2341 caaaaagttg gcaacatcaa caacatgtgg ttcttttgcc gcattttcga taatcgcctt
     2401 gccgtcggcc aaagaagccg ccatcataat attgatcgta gcgccaaccg ttacgacgtc
     2461 cagataaata tggcttccca taagccgtgt cgtttcagcc gtaatcaggc cgtgcgagat
     2521 ctttacgtct gcaccgagcg ctttaaatcc tttgatatgc tggtcgatcg gacggcttcc
     2581 gatattgcag ccccccggaa gtgctacctg ggcataatta tatttgccga gcagggcgcc
     2641 aagcatatag taggatgctc tgattttctt aatatagccg gcatcgatcg tccgattgtg
     2701 tatcgccgat gcatttactt tcacggtatg tctgtccaga tgttccacaa aaacgccgat
     2761 actttccatc gcctgtaaaa gcacattcgt atccctgaca tccggtacat tttcaattat
     2821 cactgtttca ttggacatta cggaagcggc aagaatagca agcgccgcat tttttgcacc
     2881 acctatctcc acttctccta caagagggat tccacccttt ataatgtact gttccactgt
     2941 tcgcacctct atataaagta taaattctat tttagcacaa aatatgtaat ttctcaacaa
     3001 tctaagagct attatatacc tttcgccatg tgttgtaaat caattccctt atttttggta
     3061 tttgtcacaa actcaaaact tttttaattc agataattca gcggatttac cggtgtcccg
     3121 ttcacacgga tctcaaagtg caggtgaggc ccggagctga cgcccgtatt gccgctcagt
     3181 gcgatctttt cgccctgttt cacagtctgc ccggttttaa caaggatctt gctcagatga
     3241 ccgtaacgcg tcacgattcc atccggatgc tggatatata cgcagtaacc gtatccgctt
     3301 ccccagcccg cacggatgac ctgaccgccg gaggctgcca ctacggcggt tccgatcgga
     3361 actgcccagt cgacaccttc atgataggtc gatgccccct ttgtaggtgc tgtacgtttc
     3421 ccgaatcctg aagacagacg tcctcctgat acaggcttca tataagtagg cggtgttttc
     3481 gtacccctct ccacgatttt tgccactgcc tgtgccacta catcctcata gatcaattcc
     3541 ctgctctctt cctcatcgtt gcggtatgta atatccgcaa cgacctttct gaatccggct
     3601 acaggttcct gcagaacctg tgattttgtc gtataccagg aatcattatc cacatactgg
     3661 atatcctcat catattcttc ttcataataa acctgctcgg tacgcacgac cgaaagctcc
     3721 ggctcgggca tattgacttt gatctcgtcc cccacacgga tcgtggcatt ttcatccggt
     3781 atgatttctt tgttcattgc aatgatctca tcgatcgtcg tatcgttttt ctgcgcaata
     3841 acagaaaggc tgtcaccggc ttcaacttca tagatcttgc tcttctcctg atcttttgtc
     3901 acaagctcaa tggcctcatc aagtgtactg atcttatcgc tgtccacata cgcctgcacg
     3961 acctccacat tctctccgaa atccagattt gtcacaccaa actcaaaagc cccggtttcc
     4021 tggttcaatg cctcgtcgta atattcctgg atcttctgat atgccccggc tgtcggaaag
     4081 accgaagttt cgtcttctcc ctgctcttct atctcatctg tttttgtgac ctttgttgtc
     4141 aatacattca gctgccggct tgaatcaagt acaaggtcga cggtaaactc attattttca
     4201 tcatatttgc tttttgccgt cctaagaaga gtaagcactt catttgagct ttttaaattc
     4261 acggtaaact cattgatctt cacttcatag accggctgct ttgtcttcat gatgctccgc
     4321 aaaaacacct gatatatatt gtttataacg tcctcttttg catctgtttt tccgaatatc
     4381 accctggagc cactgagtac gacttcataa ttgatcagga caagtccgtc gttttctttt
     4441 gcgatccttt ttctggcttc cagaatcatg ctgtccacct ttgatgaatc attaacaaca
     4501 ccaacctgcg ttccgttaac aaaaactgta atcagattat ttccgctctc cgaatacttc
     4561 cctatggccg gcacaagcag aaaaacaagc agtgccgtaa acagcattgc tccaatatga
     4621 gtatatttga ttatttttgt gtatttggtc agtctgcgca tattctccca tcacccgaca
     4681 tctttatatg cttgactgat agcggaaatc acatcttttg agctatcatc ggctgctgcc
     4741 cgtaataatt acgtaacaaa tttattctac caacaaatat atctgatgta aagaaaaatt
     4801 gtcgctgttt taccggcttt ccggattgtg gaaaataaat tgatgtatta aaaaaagaac
     4861 aaaaactact gagatatttt tgttcttttc gctttatttt tttcgtacgc tgtatccgaa
     4921 acttccaagc agtctgccaa gtccataata ttccccgtga gaataaacga tcggatcagc
     4981 cgcctgcaga ttaaatctgg ccccttccat ataagactca tccacatgga ccgctacgac
     5041 ctgtgccaga aa
//
LOCUS       DAOSYE010000038         5043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_11153_length_5043_cov_4.082999, whole genome shotgun sequence.
ACCESSION   DAOSYE010000038 DAOSYE010000000
VERSION     DAOSYE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11153_length_5043_cov_4.082999"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLU43_01205"
     CDS             <1..788
                     /locus_tag="PLU43_01205"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOO27053.1"
                     /translation="KALEEAGLAPEELEVIIAATVTPDKTFPFLSGEVQRELGAVHAA
                     AFDIGAACSGFLFALNTAYAYIHAGIYKNALLIGAETLSKIMDWSDRSTCVLFGDGAG
                     AAVLKADEEGVIDIVQGTDGAMGSVLSCEGRPLNNPFVKNEETAAYVQMEGQEVYKFA
                     VKTVPACIHEVLDKAGMKAEDISLFILHQANYRIIEAVAKRLRQPMDKFPTNLQECGN
                     ISAASVPILLDKINQDGKLKKGDKIVLAGFGAGLTWGACVLTW"
     gene            841..1278
                     /locus_tag="PLU43_01210"
     CDS             841..1278
                     /locus_tag="PLU43_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO27054.1"
                     /translation="MVAEKTLNSLLVELFKDIMEIEEKKLITEEFRDITYNDMHVIEA
                     IGLEDSKKMSQIAKLMSVTMGTLTKAVDSLCDKGYVERKRSRRDKRVVLVRLTERGVK
                     AYHHHETFHKEMISHILEEIDEEESKVLITTLSRLVDYFREKY"
     gene            complement(1275..2663)
                     /locus_tag="PLU43_01215"
     CDS             complement(1275..2663)
                     /locus_tag="PLU43_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOO27055.1"
                     /translation="MKTYTIQCETGESLLTALRKQHIKIAAACSGRASCGSCKIRLQS
                     GTIQAGEADRQFFSEKELADGWRLACTSYPQGSCTIEIPDIEQNKGAILTAYTEQPAS
                     IKNEEAPADFLFAAIDLGTTTIAAQLIKGNKILKTAACLNSQCSYGADVISRCEVSQH
                     GDGSILKEAVTNDIRTLLGELASAAGGRKIRRLIIAGNTVMVHLFMGYSCKGLTDFPF
                     TPVTLEQIDGELDKIPFTLFPGISTYVGGDITAGLFSCGFHKSEKVSLFVDLGTNGEM
                     AIGNKEKIICTSAAAGPAFEESKLSRATDVVLAMAGLLNKGIIDETGLLADPYFDSGY
                     PYEINGSTILISQKDIRNFQMAKAAVRAGITLLIQNYGAAYEAIDTLFLAGGMGHSLD
                     IDAAVKLGLLPKELKKRAVFSGNCSLKGTVLYGQNKCPKSLLDRIVSISTEINLANEP
                     DFQQLYFDHMSF"
     gene            3075..4565
                     /gene="pap"
                     /locus_tag="PLU43_01220"
     CDS             3075..4565
                     /gene="pap"
                     /locus_tag="PLU43_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOO27056.1"
                     /translation="MLEKVDLSKKLEKEEYKKRKEELETSLSGLQRKLREKKVPVMIV
                     FEGLGSAGKGEVINKLILPLDPRGFEVFTTGKETQEEEMHPFLWRFWTKTPERGRIAI
                     YDRSWYRKVLLDRYDGLTPQKEMEASYEEINSFEKMLADDGMLIIKFFLYISKKEQKK
                     RIRELLGNRETKWRVSKGDLDRNRHYEEFLRLHDMMLQKTDTSYAPWTIVEAEQKKYA
                     VIKVMSIVEKCFQDKLTEIENAPALVKDGKFSEGTYVTSTLKNVDLSKALSRREYEEK
                     LELLQKKLSRLHNELYTKRIPVILAFEGWDAGGKGGAIKRLTGALDPRGYTVNPTAAP
                     NDIEKVHHYLWRFWTKVPKDGHVAVFDRTWYGRVMVERIEGFCASRDWERAYKEMNDM
                     EKQFSNHGAVVLKFWMQIDKEEQERRFTERQNTPDKTWKITDEDWRNREKWDLYEQAV
                     DEMLIRTSTTYAPWVIVEGNNKYYARIKVLETVIDAIEKRLGKKSV"
     gene            4599..>5043
                     /locus_tag="PLU43_01225"
     CDS             4599..>5043
                     /locus_tag="PLU43_01225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO27057.1"
                     /translation="MMQYQVRFPQGKKKAFTLSYDDGQVYDRKLVDLFDRYGLRATFH
                     LNSGTLGIKNETDEFLRPDEIEELFDGHEISCHGVTHPFFSQLSKGQFISQIWEDRRA
                     LESYAGYPMRGMSYPYGEYSKELIAAVKELGMEYARTVEDTMNFSV"
BASE COUNT         1480 a          970 c         1334 g         1259 t
ORIGIN      
        1 aaaaagcact ggaggaagca ggacttgcgc ccgaagagct ggaggtgatt attgcggcca
       61 cagtaacgcc tgataaaaca tttccttttt tatcgggaga agtacagcgg gagcttggcg
      121 cggtccatgc ggcggccttt gatatcggcg cggcctgttc cggttttctg tttgccttga
      181 atacggcata tgcctatatt catgccggaa tttataagaa tgcgctgctg atcggtgcgg
      241 agacgctttc aaagatcatg gactggtcgg atcgttctac atgcgtactg tttggggacg
      301 gtgccggcgc agcagtatta aaggcagacg aagaaggcgt gatcgatatc gtacagggga
      361 ccgacggtgc aatgggctca gtgctttcct gcgaaggcag gccgctgaat aatccgtttg
      421 taaaaaatga ggagacggct gcctatgtgc agatggaagg gcaggaagtc tataaatttg
      481 cggtaaagac cgtacctgcc tgcattcatg aagttttgga caaagcaggg atgaaggctg
      541 aggatatcag cctgtttatt ctgcatcagg ctaattaccg gatcatagag gctgtggcaa
      601 agcgtttaag gcagccaatg gataaatttc cgaccaatct gcaggagtgc ggaaatattt
      661 ctgcggcaag tgtgccgatt ttacttgata aaatcaacca agatggtaaa ctgaaaaaag
      721 gagacaaaat cgtacttgcc ggattcgggg ctggactcac atggggggcc tgcgttttga
      781 catggtgagg actcaagcgg tttgcctgtt agcggcagat ccagacagaa gggtgatgaa
      841 atggttgcgg aaaagacatt gaacagcttg cttgtggagc ttttcaagga cattatggaa
      901 atcgaagaaa aaaagctgat cacggaggaa tttcgggata ttacctacaa tgacatgcat
      961 gtgatcgagg cgatcgggct tgaggattcc aagaaaatgt cacagatcgc aaaactgatg
     1021 tccgttacaa tgggaacact gacaaaagca gttgattctc tttgtgacaa aggctatgtg
     1081 gagcggaaga gaagcagaag ggataaaaga gttgtacttg tcaggctgac agagcgcggg
     1141 gtgaaggcct accatcatca tgaaactttt cacaaagaga tgatttcaca tattttagaa
     1201 gaaatagacg aggaagagag caaggtgctg attactacgt taagccggct tgttgactat
     1261 ttcagagaga aatattaaaa cgacatatga tcaaaatata attgctgaaa atccggttcg
     1321 ttggcaagat tgatttccgt ggaaattgaa acgatacggt caagcagaga tttcggacat
     1381 ttgttctgcc catacagaac agtgcccttt agagagcagt ttcctgaaaa gactgctctt
     1441 tttttcagct cttttggcag gagtccaagc tttacggcag cgtcgatatc aagactgtgt
     1501 cccatgccgc cggcaagaaa aagcgtgtcg atcgcctcat aagcagcacc gtaattttgt
     1561 atgagcaggg tgattccggc acggactgcg gcttttgcca tttggaaatt acggatgtct
     1621 ttttgagaga tcagtatcgt acttccgttt atttcataag ggtacccgct gtcgaaatag
     1681 ggatctgcca acaggccggt ttcatcaata atacctttat tcagaagccc tgccatggcc
     1741 agtacgacgt cggttgccct gctgagcttt gattcttcaa aggcaggtcc tgccgccgca
     1801 gaggtacata taattttttc tttattgccg atcgccattt caccatttgt cccaagatcc
     1861 acaaacaaac tgaccttttc gcttttatga aatccgcagg aaaacaaacc ggctgtaata
     1921 tcaccgccga cataagtgga gattccaggg aaaagtgtaa atggaatttt gtccagttca
     1981 ccgtcgatct gttctaacgt aacaggtgta aatggaaaat ccgtcagtcc cttacaggag
     2041 tagcccatga acaagtgtac catgaccgtg ttgccggcta tgataagccg ccgtattttt
     2101 cggccgcctg cagcagaggc aagttcgccg agcagtgtcc gaatatcatt tgtaaccgct
     2161 tcctttaaaa tactgccgtc gccatgctgt gaaacttcgc atctggatat gacatctgcg
     2221 ccatagctgc actgagagtt gagacatgcg gcggttttta gtattttgtt tcctttgata
     2281 agctgggctg cgatcgttgt tgttccgagg tcaatggcgg caaataagaa gtctgcgggt
     2341 gcttcctcat tttttatgga agccggctgt tcggtatagg cagtcaggat cgctccttta
     2401 ttttgttcga tatcaggaat ttctatcgta caggagcctt gcggataaga agtacaggca
     2461 agtctccagc cgtctgcaag ttctttttca gaaaagaatt gccggtctgc ttctccggct
     2521 tgtatcgtgc cgctttgcag gcgtatttta cagctgccgc atgaagcgcg tccgctgcag
     2581 gccgccgcta ttttgatatg ctgttttcta agagccgtta acagagactc tcctgtttcg
     2641 cattggatgg tatatgtttt cataaaaata tccttttttc ataaggtaaa aagaaagaat
     2701 gtgcgccgca cattcttccg atcagatcag tgtttcagtc ttcggtatcc tcttcacttg
     2761 caacgacggc tgaaacgacg gaaacaaccg ctgcaattac gactgcaatg acaattacta
     2821 taaatacaat tccgacaaat aaaatcataa ccgtatcctt tctgcaagag tgtatatatc
     2881 tgcatttatt atacacataa atcggaaaaa atcaaagcgt tttcgtaatt gctgaaaaac
     2941 cggtttaaaa aatacgggaa atgaaaaaat gacatattga aaagtgtaaa aatctgctat
     3001 aataatcata acagggttct gttcatatca tacgcgccgt attgcggcgc agcaggaaag
     3061 gcagggaaaa ggctatgctg gagaaggttg atttatcaaa aaaattggaa aaggaagagt
     3121 acaaaaaaag aaaagaagaa ctggaaactt cattgtccgg acttcagcga aaactgcggg
     3181 aaaagaaggt ccccgttatg atcgtatttg aaggtcttgg ttcggccggc aagggggaag
     3241 tcatcaacaa actgatactc ccgctggatc cgagaggatt tgaggtgttt acaacaggaa
     3301 aggaaacgca ggaggaggaa atgcatccgt ttctgtggcg tttctggacg aagacaccgg
     3361 aaaggggacg gatcgccata tatgaccgca gctggtacag aaaagtattg ctggaccgtt
     3421 atgacggact gacgccgcaa aaagaaatgg aggcgtccta tgaagagatc aattcttttg
     3481 agaagatgct ggcggatgac gggatgctga tcatcaagtt ctttttatat atttcaaaaa
     3541 aagaacagaa gaaaagaatc agggaattac ttggaaaccg ggaaacaaag tggcgtgttt
     3601 ccaaaggcga tctggacaga aacaggcatt atgaggaatt tcttcggctg catgatatga
     3661 tgctgcagaa aacagacaca agctatgcgc cgtggacgat cgtagaggcg gaacagaaaa
     3721 aatatgcggt cataaaagta atgtccattg ttgaaaagtg ttttcaggac aagcttacgg
     3781 agattgaaaa cgcgccggcg ctggttaagg acgggaagtt ttccgagggc acctatgtta
     3841 caagcacact gaaaaatgtt gatttgtcca aagcactgtc aagaagagag tatgaggaaa
     3901 aactggagct gctgcagaaa aagctttcca ggctgcataa tgaattatat acgaaaagaa
     3961 ttccagtgat ccttgcgttt gaaggctggg atgccggcgg aaaaggcggt gcgatcaaac
     4021 ggctgacagg tgcgcttgac ccgaggggct atacggtgaa tccgacagcg gcgccgaatg
     4081 atattgaaaa ggtacatcat tatttgtggc gtttctggac aaaagtaccg aaggacggtc
     4141 atgtggctgt ttttgacaga acatggtatg gcagggtcat ggtcgagagg atcgaaggct
     4201 tttgtgcatc tcgtgactgg gaacgggctt ataaggaaat gaatgatatg gagaagcagt
     4261 tttctaacca tggagcggtt gttttgaaat tctggatgca gatagacaag gaggaacagg
     4321 aacggcggtt tacggagcgg cagaacacac cggacaaaac atggaagatc acagatgaag
     4381 actggagaaa ccgtgaaaaa tgggatctgt atgaacaggc cgtggacgaa atgctgatcc
     4441 gtacttccac gacgtatgca ccatgggtca ttgttgaagg aaataataaa tattatgcga
     4501 gaatcaaagt gcttgaaacg gtaatagacg caatagaaaa gagacttgga aaaaaatccg
     4561 tatagaaata aaataatgga gaacaggaag gaaatggtat gatgcaatat caggttcgct
     4621 ttccgcaggg aaagaaaaaa gcatttacgc tgagttatga tgatggtcag gtctacgacc
     4681 ggaagctggt ggatctattt gacaggtatg gtctgcgcgc gacgttccat ctgaattcgg
     4741 ggacgcttgg aataaaaaat gagacggacg agtttctgcg tccggatgag atcgaggagc
     4801 tgtttgacgg acatgaaatt tcctgtcatg gcgttacaca tccttttttt tcacagcttt
     4861 ccaaaggaca gtttatatca cagatctggg aagacagacg ggcgctggaa agctatgcgg
     4921 gctatccgat gcgcggaatg tcttatccgt atggggaata ttccaaagaa ctgattgctg
     4981 cagtaaaaga gctggggatg gagtatgcga gaacggtcga agatacgatg aatttttctg
     5041 ttc
//
LOCUS       DAOSYE010000039         5029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_11192_length_5029_cov_3.398271, whole genome shotgun sequence.
ACCESSION   DAOSYE010000039 DAOSYE010000000
VERSION     DAOSYE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11192_length_5029_cov_3.398271"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..316
                     /locus_tag="PLU43_01230"
     CDS             71..316
                     /locus_tag="PLU43_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO27058.1"
                     /translation="MSDNGMGIPEETIETLLTKKAKTRGKGSGIGLWNVNQRISLYFK
                     GDYGLSISSELDEGTTVTIHLPKIPYEQQHKGEGVPS"
     gene            313..1281
                     /locus_tag="PLU43_01235"
     CDS             313..1281
                     /locus_tag="PLU43_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO27059.1"
                     /translation="MKIGRRFLTICISIILILIVAEFYYYSNYTTKKEPVFNISLIVY
                     GNSTDRWENLKQGAERAADGTSAEINLITMSSDLNAKEQISLINREIENGADALMIAA
                     CDSEKIEEYFKGLSVKIPVVFVQNGFEDNTDTWYISADNYAMGHTLGNVIAANEKSWI
                     KAAIISDRTERKNVADRFDGVYDSLVQYADEVVIWERNENERDSQTMLFLQRELTEEA
                     VDVVVALDTSSMEALMDAIENLNKNIKVYGIANSDKAVYYLDNGKIKALIFQNEFSIG
                     YLGVKVILNETAYNKKRIFNLIKYETVNKDNMYSEEYQKLLFPFVK"
     gene            1300..2376
                     /locus_tag="PLU43_01240"
     CDS             1300..2376
                     /locus_tag="PLU43_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO27060.1"
                     /translation="MKQKKKMRMLTILLALVLLFSLTGCQFGKLLVSKEESDPKSIKI
                     GISIYDEYDTFISSIVQDIDEWSKEKEKETGITITLEVVSASGSQLTQNDQVEKFVDK
                     SCNVICVNLVDRTDASVIIDKAKNADIPVIFFNRELVKEDLERWDKLYYVGAVAEQSG
                     KLQAKVVTDALSDEKIFHETDVNDDGTIQYVMLEGETGHQDALVRTQVSIEEIKKAGY
                     SVEKLGDEIANWNRAQAATKMTALLERYPWQIEMIIANDDDMALGAIDALEAADAEHW
                     PMIVGINGTKDALEMIKTKKMEGTVYNDAKGQAELIMKMAYELAVDRKISDDIELEDG
                     KYGYLPYRMINYDNVQEYIRLNAQ"
     gene            2424..3533
                     /locus_tag="PLU43_01245"
     CDS             2424..3533
                     /locus_tag="PLU43_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO27061.1"
                     /translation="MPENDAGKSKYYVVMENLRQRMMSGKLRAGNKLPSENELVKEFD
                     VSRHTVRKALSILEREGYIEAIHGKGTYCIERLRRKESSHNIAVVTTYISDYIFPRLI
                     QGIDNVLTKEGYSIILKNTGNSQKNEAKALEDIITKNIDGLIIEPSKSEIFCRHMHQY
                     EQLDRLGIAYVFLQGTYPQMKEKPNIVMDDVAGGYLLTKYLIELGHRHLIGIFKIDDS
                     QGVARQKGFIKALNEAGLPYDPDDVILFHTEDRARKPAGMMKQFLDEKKQMDAVVCYN
                     DQIALEVIRVLEAEGLKVPQDLSVTGYDNSFIAENGPVRLTTISHPKERLGEMVAKLL
                     LEKINGVEDKDSKVERVIKPELVIRDSCMDRNRTS"
     gene            3656..>5029
                     /gene="araA"
                     /locus_tag="PLU43_01250"
     CDS             3656..>5029
                     /gene="araA"
                     /locus_tag="PLU43_01250"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOO27062.1"
                     /translation="MKTGKDYKFWFCTGSQDLYGDECLAKVAEHSKIIVEHLNKSGKL
                     PFEIVWKPTLITNELIRKTFNEANADDECAGVITWMHTFSPAKSWILGLQEFRKPLCH
                     FHTQFNQEIPYDTIDMDFMNENQSAHGDREYGHIVTRMGIERKIIVGHWDTDKAKEEL
                     AGWMRTAIGLMESSHIRVCRIADNMRNVAVTEGDKVEAQIKFGWEIDAYPVNEIVEFV
                     KAVPQGEISALTEEYYDKYEILLEGRDAGEFKKHVAVQAGIEIGFEKFLEEKNYQAIV
                     THFGDLGGLEQLPGLAIQRLMEKGYGFGGEGDWKTAAMVRLMKLMTTGIKDAKGTSFM
                     EDYTYNFVPGREGILQAHMLEVCPTIADGPISIKVNPLSMGEREDPARLVFTSKTGPA
                     IATSLIDLGTRFRLIINEVECKKAEKPMPKLPVATAFWTPQPDLMIGAQAWILAGGAH
                     HTAFSYDL"
BASE COUNT         1630 a          915 c         1326 g         1158 t
ORIGIN      
        1 tagcaggaag aactatgttc gtaatccgtc atcccacaaa atttgtgatt gagccgatat
       61 ttatatagaa atttccgata acggcatggg aattcctgag gaaacgatag aaacgctctt
      121 gacgaaaaag gctaaaacaa ggggaaaggg ttccggaatc ggactctgga atgtcaatca
      181 gcgaatcagt ttatatttta aaggcgatta tgggttatcg atcagcagtg aactggatga
      241 gggaacgact gttacgatcc accttccgaa gattccttat gagcagcagc ataaaggaga
      301 gggggtgccg tcatgaaaat aggcagacgt tttctgacga tctgtatttc gatcatttta
      361 atactgatcg tagccgaatt ttattattac agtaactata cgacgaaaaa agaaccggtc
      421 ttcaatatat ccctgatcgt atacggaaac agtaccgacc ggtgggaaaa tctgaaacag
      481 ggcgcggaac gggcggcaga cggtacttcc gcagaaatca atctgatcac gatgtcttct
      541 gacctgaacg caaaagagca gatctccctg atcaacaggg aaattgaaaa cggcgccgat
      601 gcattgatga tcgcagcctg cgacagtgaa aagatagaag aatattttaa gggactcagt
      661 gtaaaaattc cggtcgtgtt cgtacagaac ggatttgaag acaatacgga cacgtggtat
      721 atttccgccg ataattacgc aatgggacac acgcttggga atgtgatcgc ggcaaacgaa
      781 aagagctgga tcaaagccgc tatcatttct gacagaacag agagaaaaaa tgttgcggac
      841 cggtttgacg gcgtatatga ttccctggtc cagtatgcgg atgaagtcgt gatatgggaa
      901 aggaacgaga atgaaagaga cagccagacc atgctctttt tgcagagaga actgacggaa
      961 gaagcggtcg acgttgtggt ggcacttgat acaagctcta tggaagcgct gatggacgcg
     1021 atcgaaaatc tgaataagaa tatcaaggta tatggaattg caaacagtga taaagccgta
     1081 tactatctgg ataacggtaa aataaaagcg ctgatctttc agaatgaatt cagcattggg
     1141 tatcttgggg tcaaggtcat actcaatgaa acggcataca ataagaaaag aatatttaat
     1201 ctgatcaaat atgaaaccgt caataaggat aacatgtatt cggaagaata tcaaaagctg
     1261 ttgtttcctt tcgtaaaata gctttttgtg gggtgggaaa tgaaacaaaa gaaaaaaatg
     1321 agaatgctca caatactgct tgcactggtc ctgctgtttt ccctgaccgg ctgtcaattt
     1381 ggcaaactat tggtcagcaa agaagaaagt gatcccaaaa gcataaaaat aggaatcagt
     1441 atttatgatg aatatgatac attcatttcg agtatcgtac aggatattga cgaatggagc
     1501 aaggaaaaag agaaggagac aggtataacg atcaccctgg aagtcgtgag tgcaagcgga
     1561 agtcagctga cacaaaatga tcaggtggaa aaatttgttg ataaaagctg taatgtgatc
     1621 tgtgtcaatc ttgtggaccg gacagacgca tctgtaatta ttgataaggc aaaaaacgcg
     1681 gatattccgg taattttttt taacagggag ctggtaaaag aggatctgga gcgatgggat
     1741 aagctttatt atgtaggtgc tgttgccgaa cagtccggaa agctgcaggc aaaggtcgtc
     1801 acggatgcat tgagcgacga aaaaatattt catgaaacag atgtaaatga tgacggcacg
     1861 atccagtatg tcatgcttga aggagaaacc ggacatcagg acgcgctggt gcggacgcag
     1921 gtttccattg aagaaatcaa aaaagcagga tattccgtgg aaaagcttgg agatgagatc
     1981 gccaattgga acagggcgca ggcggcgacc aaaatgacgg cgctgcttga aagatatcca
     2041 tggcagatcg aaatgatcat tgcaaatgac gacgatatgg cacttggggc gatcgatgca
     2101 ctggaagctg cggatgctga gcactggccg atgatcgtcg gtattaacgg aaccaaagat
     2161 gcgcttgaaa tgatcaagac gaaaaagatg gaaggcacgg tctataatga cgcaaaagga
     2221 caggcagagc tgatcatgaa aatggcatat gagcttgccg tggacaggaa aatatcggat
     2281 gatatcgaat tagaagacgg aaaatacggg tatcttccat accgtatgat caattatgac
     2341 aatgtacagg aatacatcag actgaatgca cagtagcttt tttggaggat atccggaagc
     2401 aggctgcaat tgaggaaacg aacatgccgg aaaacgatgc gggaaaatca aaatattatg
     2461 ttgtaatgga aaatctgcgg cagagaatga tgagcggaaa actcagggcg ggaaacaagc
     2521 ttccgtctga aaatgaactc gtaaaggaat ttgacgtcag ccgccatacg gtgcggaaag
     2581 ccctttctat tctggaaagg gaaggctaca tagaagcgat ccatggaaaa gggacgtatt
     2641 gtatcgaacg gctccggcgc aaagagagct ctcataatat tgccgttgta acgacgtata
     2701 tttccgatta tatatttccg cggctgatcc aggggatcga taatgtgctg acaaaggaag
     2761 ggtacagcat tattttgaaa aataccggga acagccagaa aaacgaagca aaagcgctgg
     2821 aggatattat aacaaaaaat attgacggac tgatcataga gccgagcaaa agtgagatct
     2881 tctgccggca tatgcatcag tacgagcagc tggaccgcct gggtattgca tatgtgttct
     2941 tacagggaac ctacccgcag atgaaggaga agcccaatat tgttatggac gatgttgcag
     3001 gaggatatct gctgacaaaa tatttaatag aactgggaca ccggcatctg atcggtattt
     3061 ttaaaattga cgacagtcag ggtgttgcca gacagaaagg ttttatcaag gcactgaatg
     3121 aagccggact cccctatgat ccggatgatg tgatcttatt tcatacggaa gaccgggcga
     3181 ggaagccggc cggcatgatg aagcagtttc tggatgagaa aaagcagatg gatgctgttg
     3241 tctgttataa tgatcagatc gcgcttgagg tcatccgggt attggaggcg gaagggttaa
     3301 aagtgccgca ggatctgtct gttaccggct atgataattc atttatagcg gaaaacggac
     3361 cggtcaggct gacgacgatc agtcatccca aggaacggct cggggaaatg gtggcaaaat
     3421 tactgctcga aaaaatcaat ggagttgagg acaaggacag taaagtggaa cgtgtcataa
     3481 agccggaatt ggtcatcagg gattcctgta tggacagaaa tcgaacaagt taataaaaat
     3541 ttattaaaaa gcaggcaatt agagggaaaa acaacttgta cacaaaacca tacaagtttt
     3601 ataatttctt atagaaggta ctataaaaat attaaattca ggagggtttc aaagcatgaa
     3661 gacaggaaaa gattacaaat tttggttttg tacgggttca caggatttat acggagacga
     3721 atgtcttgca aaagtggcgg agcattccaa gatcatcgta gagcatttaa acaaatcagg
     3781 gaagctgccg tttgaaattg tctggaagcc gacgcttatt acaaatgagc tgatcagaaa
     3841 aacatttaac gaggcaaatg cagatgacga atgcgcaggc gttattacat ggatgcatac
     3901 attttctcct gcgaaatcgt ggatcctcgg actgcaggaa ttcagaaaac cgctgtgcca
     3961 tttccacaca cagttcaatc aggaaattcc atatgacacg atcgatatgg acttcatgaa
     4021 tgaaaatcag tccgcacacg gagacagaga atacggacat atcgttacgc gcatgggaat
     4081 tgaaagaaag atcatcgtcg gacactggga taccgataag gcaaaagaag agcttgcagg
     4141 ctggatgaga acagcgatcg gtcttatgga atcttcacat atcagagtat gtcgtattgc
     4201 ggacaatatg agaaatgttg ccgttacgga aggggataaa gttgaggcac agatcaaatt
     4261 cggctgggag atcgacgcgt atccggtcaa cgagatcgta gaatttgtga aagccgttcc
     4321 gcagggagag atcagtgcgc ttacggaaga atattacgac aaatatgaaa ttctgctcga
     4381 gggcagggat gccggcgaat ttaaaaagca tgttgctgta caggccggta ttgaaatcgg
     4441 atttgaaaag tttttggaag aaaaaaatta tcaggcgatc gtaacacatt tcggcgatct
     4501 tggaggcctg gaacagctgc cgggtcttgc tattcagcgg ctgatggaaa aaggatatgg
     4561 attcggcggt gaaggcgact ggaagactgc ggcaatggta cgtctgatga aacttatgac
     4621 gaccggtatc aaggacgcaa agggaacctc gttcatggaa gattatactt ataattttgt
     4681 tccgggcagg gaaggcattt tacaggcgca tatgctcgaa gtatgtccga cgatcgcgga
     4741 cggaccgatc agtatcaaag taaatccgct ttccatggga gaaagggaag atccggcgag
     4801 acttgtattt acgtcaaaga ccggtccggc tattgcaaca tccctgatcg atctcggaac
     4861 ccggttccgt ctgatcatta atgaagtgga atgtaaaaaa gccgaaaagc cgatgccgaa
     4921 gcttccggta gctaccgcat tctggacacc gcagcctgat ctgatgatcg gggcacaggc
     4981 gtggatcctg gcgggcggtg cgcatcatac cgcattttct tacgatctg
//
LOCUS       DAOSYE010000040         4913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_11580_length_4913_cov_2.821120, whole genome shotgun sequence.
ACCESSION   DAOSYE010000040 DAOSYE010000000
VERSION     DAOSYE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11580_length_4913_cov_2.821120"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..926
                     /gene="araD"
                     /locus_tag="PLU43_01255"
     CDS             240..926
                     /gene="araD"
                     /locus_tag="PLU43_01255"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOO27063.1"
                     /translation="MLKRLKEQVYEANMALPKYGLVTFTWGNVSGIDREKGLFVIKPS
                     GVEYDSLKPEDMVVVDLEGEIVEGNYKPSSDTPTHLELYKAFPEIGGIVHTHSSFATS
                     WAQSGRGIPCYGTTHADYMYGEIPCARSLTAEEIHSEYEKNTGLLIIETFKEKDVMAV
                     PAVLCANHGPFTWGKNAKEAVHNAVVLEEVAKMAYRTELINTDVQPAPQVLQDKHYYR
                     KHGANAYYGQ"
     gene            952..1788
                     /locus_tag="PLU43_01260"
     CDS             952..1788
                     /locus_tag="PLU43_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO27064.1"
                     /translation="MNKLELQKTANEIRKGIVTAVHAAKAGHPGGSLSSADIMTYLYF
                     EEMNIDPENPQKEDRDRFVLSKGHCAPALYSTLAQRGYFPKEELVTLRKLGSHLQGHP
                     CMQHTKGVDMSSGSLGQGISVATGMALSAKLSNDAYRVYTLLGDGELAEGQVWEAAMF
                     AGHKKLDNLVVIVDNNGLQIDGPVTEVCNSYPIDEKFKAFNFHVINCSAHDFNELEAA
                     FEEAKNTKGMPTAIIAKSTKGKGVSFMENSVKWHGVAPDDEQYAVAMADLEKVGEALC
                     QK"
     gene            1776..2720
                     /locus_tag="PLU43_01265"
     CDS             1776..2720
                     /locus_tag="PLU43_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOO27065.1"
                     /translation="MSEVKKIATRVSYGNALVELGNEHEDLVVLTADLAAACKTDIFR
                     KAHPDKHINCGIAEANMTCIAAGLAATGKVPFASSFAMFSAGRAFEQVRNSIGYPHLN
                     VKIGATHAGISVGEDGASHQCNEDIALMRTIPGMVIISPADDVEAKAAVKAAYEYKGP
                     VYLRFGRAAVPVFNDEESYQFEMGKGIILKEGKDVTIVATGLTVPESMEAAKLLAADG
                     IDAEVINIHTIKPLDEELIVASAKKTGKVVTAEEHSVIGGLGSAVCDVLCEKLPTPVK
                     KIGMQDVFGESGSASALIHKYQLDAEGIYKQIKTFMEA"
     gene            2882..3283
                     /locus_tag="PLU43_01270"
     CDS             2882..3283
                     /locus_tag="PLU43_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOO27066.1"
                     /translation="MKIVVIDGQGGNIGRMLIERLIKKLPDAQIVAVGTNGIATANML
                     KAGVRLGATGENATIVNARDADYIIGPIGIVIADSLLGEVTPAMAAAVGGSPAKKVLI
                     PSLKCNNYVVGTKEIALDRLIELAAEEITEV"
     gene            3345..3944
                     /locus_tag="PLU43_01275"
     CDS             3345..3944
                     /locus_tag="PLU43_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO27067.1"
                     /translation="MRNLITVFYERIISMNQKTKKLTLSGVCLALCLVLPFLTGQIPQ
                     IGSALSPMHIPVLLCGFICGWQYGLVVGLIAPVLRFLLFQMPPIFPTGTAMAFELAVY
                     GAVTGILYHRLPKKNLNIYIALISAMLAGRIVWGCVQLVLSGVTKTPFTWQAFLSGAF
                     IVAVPGIICHILLIPPIVIALKKAGVIEDEGNRKDYQAA"
     gene            3910..>4913
                     /locus_tag="PLU43_01280"
     CDS             3910..>4913
                     /locus_tag="PLU43_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27068.1"
                     /translation="MKEIEKIIRQHSKQYPKMQPQDVAKLIYQNEFGAGHFVDNAADS
                     LQRLQDEYGRERSNTKEPVIERIGNGYVRYALSGVKENELETLNRLFVLTAGQTGSKK
                     GRKENYEEKLYSVLSFFSCSEFSFTKEEYRAYIKTQKEKGYPAVSHSEIYRQAYTPVY
                     RVIDERFSPYVELLAELEEQYQKKCGGENCLTVGIDGNAAAGKTTLAWCISELYDCEV
                     IHMDDFFLPPHLRTGERLSEPGGNIHYERFLQEVVAGIKSKKDFSYRIFSCHKMDYTG
                     ERTVKRQKMIVVEGAYSMRPEFRDIYDYKIFIRLPLETQLQRIQIRNGPQMCRIFEEQ
                     "
BASE COUNT         1499 a          930 c         1303 g         1181 t
ORIGIN      
        1 attttcttac gatctgacaa cagaacaaat ggttgactgg gcggctgtaa tgggcatcga
       61 aagcgttgta atcgacaaaa atacaacgat ccgcaatttc aagaatgaac ttagatggaa
      121 tgaagcagtc tttagatgat ctgtccgcag cgggaaatac cgggtttgtt taatcaatga
      181 actaattatt gaaacagtta tgatagtgtg ctatactacg tacgaaagga tgaaggaaca
      241 tgttaaagcg tttaaaagaa caggtttatg aggcaaacat ggcgcttcca aagtatgggc
      301 tcgttacctt tacatgggga aatgtcagcg ggatcgacag ggagaaaggt ctttttgtta
      361 tcaagccaag cggtgtagag tatgattccc tgaagccgga ggatatggtc gttgtagatt
      421 tggaaggaga gattgtggaa ggaaattaca agccgtcttc cgatacgccg acacatctgg
      481 aactctataa ggcattcccg gaaataggag gcatcgtaca tacccattct tcctttgcga
      541 cctcctgggc gcagtcggga agagggattc cctgctatgg tacgacacat gcggattata
      601 tgtatgggga gatcccatgc gcgagaagcc ttacggcgga ggaaattcat tccgaatatg
      661 aaaaaaacac aggacttctg atcattgaaa cgtttaagga gaaggacgtt atggcagtgc
      721 ctgctgtact ctgtgcaaat cacggaccgt ttacatgggg aaaaaatgca aaagaggccg
      781 tacataatgc ggtcgtattg gaagaggttg ccaaaatggc atatcgtacg gagctgatca
      841 acacagatgt gcagcctgcg ccgcaggtat tgcaggacaa gcattattac aggaagcatg
      901 gtgcgaatgc ttattacgga caatgatcat aatttagaaa ggacgattat catgaacaag
      961 ttggaattac aaaaaacagc caatgaaatc cgcaagggta ttgtaacggc ggttcatgcc
     1021 gcaaaagccg gacatccggg cggctcgtta tcttcggcag atattatgac atatctttat
     1081 ttcgaagaaa tgaatattga cccggaaaat ccgcagaaag aagacagaga ccgttttgtt
     1141 ttgtcaaaag gacactgtgc gccggcgctt tattctacgc ttgcacagcg cggatatttt
     1201 ccaaaagagg agcttgtgac cctgcggaaa ctcggttctc acctgcaggg acatccatgt
     1261 atgcagcata cgaaaggtgt tgacatgagc agcggttctt taggacaggg gatttccgtt
     1321 gcgaccggta tggcgctttc cgcaaagctt tcaaatgatg cctacagagt ttatacactg
     1381 cttggagacg gcgagcttgc agaaggccag gtatgggaag ctgcaatgtt tgcaggacat
     1441 aagaagctgg acaatcttgt ggtaatcgta gacaataacg gacttcagat cgacggaccg
     1501 gtcacggaag tatgtaattc ttatccgatc gatgaaaagt tcaaagcatt caatttccat
     1561 gtaatcaatt gcagtgcgca tgattttaac gaacttgaag cagcattcga ggaagcaaaa
     1621 aatacaaagg gcatgccgac agccattatt gcaaagagca cgaagggcaa aggggtttct
     1681 ttcatggaaa acagtgtaaa atggcatggc gtagcgcctg acgatgagca gtatgccgtt
     1741 gcaatggcag atcttgaaaa ggtaggtgaa gcgttatgtc agaagtaaag aagatcgcaa
     1801 caagagtaag ctatgggaat gcactggtcg aacttggaaa tgaacatgag gatcttgttg
     1861 tattaactgc ggacttagcc gcagcctgca aaaccgatat tttcaggaag gcacatccgg
     1921 ataaacatat caactgcgga atcgcggaag ccaatatgac atgtattgcg gccggacttg
     1981 cagcaacagg caaagttccg tttgcaagtt cgtttgcgat gttttccgca ggaagagctt
     2041 ttgaacaggt aagaaacagt atcggatatc cgcatctgaa tgtaaagatc ggagcgacac
     2101 atgccggtat ttccgtaggc gaggacggtg catctcatca gtgtaacgag gatattgccc
     2161 tgatgagaac gattccgggg atggtcatta tttctccggc agacgatgtg gaagccaaag
     2221 cggccgtgaa ggcagcttat gaatataaag gaccggtata tctgcgtttc ggacgtgcgg
     2281 cagttcccgt gttcaatgat gaagaatcct atcaatttga aatgggaaaa ggaatcatat
     2341 taaaagaagg aaaagatgtg acgatcgtag caaccggact gaccgttccg gaatccatgg
     2401 aagcagcaaa actgcttgcg gcagacggca ttgatgcgga agtcatcaat attcatacga
     2461 tcaagccgct ggatgaagaa ctgatcgttg cttccgccaa aaagacaggc aaagtcgtta
     2521 cggcagaaga acattcggtg atcgggggac ttggcagtgc tgtatgtgat gtgctttgcg
     2581 agaagctgcc gacaccggtt aaaaagatcg gtatgcagga tgtgttcggg gaatccggtt
     2641 ctgcatccgc actgatccat aaatatcagc tggatgcgga aggaatttat aaacagatca
     2701 aaacgtttat ggaagcataa tagttagaaa tcaaacaggt ttatacagga cataagtaaa
     2761 acagtgcggg atattttccc gtactgtttt taaaataaat cgtaaaaaag tattgacata
     2821 gagccgggtt caggtgatag actaataata aatgagtaca ggaagtactc gcggaggaaa
     2881 catgaaaatc gtagtaattg acggacaagg cgggaatata ggacgcatgc tgatcgagcg
     2941 gctgataaaa aagctgcccg atgcacagat cgttgcggtc ggaacaaatg ggatcgccac
     3001 ggcgaatatg ctcaaagcag gcgtacggct tggtgcaaca ggagaaaatg caacgatcgt
     3061 taatgcacgt gatgccgatt atatcattgg cccgatcggt attgtgatcg cagattctct
     3121 tttgggagaa gtcactccgg caatggcggc agcggtcggc gggagcccgg caaaaaaagt
     3181 tctgataccg agtctgaaat gtaataatta tgtggtcgga actaaagaga tcgccctcga
     3241 caggctgatc gagctggcag cggaagaaat tacggaagta taggtgaagg gataaccctt
     3301 ttataataat accaggaagg tataaaacgc ttcagaagaa gtgaatgaga aacctgataa
     3361 cagtatttta tgaaaggatt ataagtatga atcaaaaaac aaaaaaatta actttatcag
     3421 gagtatgtct ggcgctttgc ctcgtactcc cttttttgac agggcagatt ccgcagatcg
     3481 gaagcgcatt gagcccgatg catataccgg tgctgctgtg cgggtttatc tgcggctggc
     3541 agtatggtct ggtcgtaggc ctgatcgcac ctgtgttaag atttttgctg tttcagatgc
     3601 cgccgatctt tccaacagga acggcgatgg catttgagct tgcagtttac ggggcagtga
     3661 caggaatttt gtatcacagg cttccaaaga aaaatttgaa tatttatatt gcattgatca
     3721 gtgcaatgct ggccggaagg atcgtatggg gctgtgtaca gctcgtgctg tccggcgtta
     3781 caaaaacacc gtttacatgg caggcatttt tatcgggcgc gttcatcgtt gcggtaccgg
     3841 gaattatctg tcatattctg ttgattccgc cgatcgtcat agcactgaag aaagcgggag
     3901 taatagaaga tgaaggaaat agaaaagatt atcaggcagc atagtaaaca atatccgaaa
     3961 atgcagccgc aggatgtggc aaaactgatc tatcagaacg aattcggagc cggtcatttt
     4021 gtggataatg ccgcagattc cctgcaaaga ctgcaggacg aatatggacg ggaaagatca
     4081 aatacaaagg aacctgtcat agaacggatc ggaaacggat atgtgcggta tgcgctgtca
     4141 ggagtgaaag aaaatgaact ggagacgctg aaccggctgt ttgtattgac agccggtcag
     4201 accggatcaa aaaaaggcag aaaggaaaat tacgaagaga aattatattc ggttctgtct
     4261 tttttttcgt gttctgaatt ttcttttaca aaagaagaat acagagctta tataaagacc
     4321 cagaaagaaa aaggataccc tgccgtaagc cacagtgaaa tttacagaca ggcgtataca
     4381 cctgtatacc gtgtgatcga cgaacggttt tctccatatg tggagctgct tgccgaactg
     4441 gaagaacagt atcagaaaaa atgcggcggg gaaaactgtc tgaccgttgg gattgacgga
     4501 aatgcggcag caggcaaaac aacacttgcc tggtgtattt cagaattata tgactgtgaa
     4561 gtgatccata tggatgactt ttttctgccg ccgcatttac ggacaggcga gcgcttgtcg
     4621 gaaccgggcg gaaacattca ctatgagcgc tttttgcagg aagtggttgc aggaattaaa
     4681 agcaaaaaag acttttctta tcggatcttc tcctgccata aaatggacta taccggagaa
     4741 cgtacggtaa aacgacagaa aatgatcgtg gtggaaggtg cgtattccat gcggccggaa
     4801 tttcgggata tttatgatta taagatcttt atcaggctgc cgttagaaac acagcttcaa
     4861 agaattcaga tccggaacgg tccgcagatg tgccgtattt ttgaagagca atg
//
LOCUS       DAOSYE010000041         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_11965_length_4804_cov_3.147399, whole genome shotgun sequence.
ACCESSION   DAOSYE010000041 DAOSYE010000000
VERSION     DAOSYE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11965_length_4804_cov_3.147399"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="pflA"
                     /locus_tag="PLU43_01285"
     CDS             complement(<1..679)
                     /gene="pflA"
                     /locus_tag="PLU43_01285"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOO27069.1"
                     /translation="MKGRIHSLESFGTVDGPGVRYVVFFQGCPMRCLYCHNPDTWTMT
                     GGTEMEAAQIIEQYERNKSFYKNGGITATGGEPLMQIDFLTELFAEAKRRGIHTCLDT
                     SGFPYNESDPVMMEKMDRLMEVTDLIMLDIKHIDPILHKKLTAQPNSNILAFAEYTAM
                     KGTDLWIRHVVVPGFTDDDNYLFQLGYFIGGLTNLKALDVLPYHTMGKAKYKQLNFNY
                     PLADTPPM"
     gene            complement(705..947)
                     /locus_tag="PLU43_01290"
     CDS             complement(705..947)
                     /locus_tag="PLU43_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOO27070.1"
                     /translation="MTENNMQIENLVSLLDGYSANGGHHLNVNVLNRETLLNAQKHPE
                     EYPQLTIRVSGYAVNFINLTPEQQNDVISRTFHDVM"
     gene            complement(979..1182)
                     /locus_tag="PLU43_01295"
     CDS             complement(979..1182)
                     /locus_tag="PLU43_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27071.1"
                     /translation="MDENKMIDDLISVLDHSVGNGVGHLNVEVDPNGDTMRTVETMGC
                     TDCSKTPQACSIPTLHQGLDDYE"
     gene            complement(1203..3251)
                     /gene="pflB"
                     /locus_tag="PLU43_01300"
     CDS             complement(1203..3251)
                     /gene="pflB"
                     /locus_tag="PLU43_01300"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOO27072.1"
                     /translation="MIITKSEWNGFAGGKWENEINVRDFIQKNYAPYDGDDSFLAAPT
                     QNTKDLWAQVLDLQKQERAAGGVLDMDTKIVSTITSHGPGYLDKDKETIVGFQTDRPF
                     KRSLQPYGGIRMAEKACSDNGYEVAPEISEFFSIHRKTHNAGCFDAYNTEMRACRSSH
                     IITGLPDAYGRGRIIGDYRRIALYGIDRLIEDKKAQRSSTGRVMTDDVIRLREELSEQ
                     MTALKMMKEMAASYGCDISGPSKNVQEAIQATYFGYLCAVKEQNGAAMSLGRTSTFID
                     CYAERDLRNGTFTEEQIQEFVDHFIMKLRCIKFARTPEYNQIFAGDPVWVTESIGGIG
                     IDGRHLVTKMSFRYLHTLNNLGAAPEPNLTVLWSPRLPENFKRFCAKTSINTSSIQYE
                     NDELMRITHGDDYGIACCVSSMVIGKEMQFFGARANLAKCLLYALNGGVDEVTKKQIG
                     PKYRPVEGDVLDYDDVLAKFMDMMEWQADVYVNTLNIIHYMHDKYCYERAQMALHDRD
                     VKRYFATGIAGLSIVADSLSAVKYAKVEVIRDEDGIITDFRITGDFPKYGNDDDRVDT
                     IAQHVVSRFMTAIRRHHTYRDGFPTMSVLTITSNVTYGKATGNTPDGRKMGQPFAPGA
                     NPMHGRDSNGAVASLSSVAKLPFDDAQDGISNTFTIIPGALGKEDRVFAGDIEFELSK
                     "
     gene            3605..3805
                     /locus_tag="PLU43_01305"
     CDS             3605..3805
                     /locus_tag="PLU43_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOO27073.1"
                     /translation="MAAITKDMTIGQAITVNQEIIPVLLDIGMHCIGCPSAQGETLEE
                     AAMVHGMDPDELMEKISEAVGE"
     gene            complement(3877..4629)
                     /locus_tag="PLU43_01310"
     CDS             complement(3877..4629)
                     /locus_tag="PLU43_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HOO27074.1"
                     /translation="MKLEKVNDHQIRCTLTKADLADRELKISELAYGTEKAKSLFRDM
                     MQQAAYEFGFEAEDIPLMIEAIPLSADCIVLIITKVEDPEELDTRFSNFAPSVHDEED
                     TGLDEVIQSIGDDTDNVLDLFKKLKQERTPASTGLVEEAKEKLLEKTSAAYELSKAYS
                     FNDIDAVARLAHVMKTFYFGRNSLYRDRKSGRVVLIVSKSIHTQEDFNRICNMISEYG
                     TPEKYSPAAEAYFEEHNELILKEDALQKLLNV"
BASE COUNT         1247 a         1036 c         1031 g         1490 t
ORIGIN      
        1 ccatgggggg tgtgtcggca agcggatagt taaagttaag ctgcttgtat ttggcttttc
       61 ccattgtatg ataaggaagg acatcaagcg ccttgagatt tgttaatccg ccgataaaat
      121 agccgagctg aaacaggtaa ttgtcgtcgt cggtaaagcc cggaacaacg acatgacgga
      181 tccaaagatc cgttcccttc atggcagtat attctgcaaa agcaagaata ttgctgtttg
      241 gctgtgcagt cagttttttg tgcagaatgg gatcaatatg cttgatgtcc agcatgatca
      301 gatcagttac ttccatcagt ctgtccattt tttccatcat aacaggatcg ctttcattgt
      361 atggaaatcc ggaagtatca agacatgtat ggatgccgcg gcgttttgcc tctgcaaaca
      421 gctctgtcag aaagtcgatc tgcataaggg gttcgcctcc ggttgccgtg atacctccgt
      481 ttttataaaa gcttttgttt ctttcatatt gttcaatgat ctgtgcagct tccatttccg
      541 tgccgccggt cattgtccat gtgtcaggat tatggcagta aagacatcgc atggggcatc
      601 cctgaaaaaa aacaacgtaa cgtacaccgg gaccgtctac ggttccaaag ctttctagag
      661 aatgaatgcg gcctttcatg agatcgcctc ctgttgtttt gcaattacat tacatcatga
      721 aatgtccgtg aaataacgtc attctgttgt tccggtgtca gattgataaa gtttaccgca
      781 tatccggaaa cgcgaatcgt cagctgcgga tactcttcag ggtgcttttg tgcatttaat
      841 aaagtttctc tgtttaatac atttacattt aaatgatgcc cgccgtttgc agaatagcca
      901 tccagcagtg atacaagatt ttcaatttgc atattatttt ctgtcataat agtttctcct
      961 ttaaattaga ctttcgtttt attcataatc gtccagaccc tggtggagcg tcggtatgct
     1021 gcaggcctgc ggtgtttttg aacaatctgt gcagcccatt gtttctactg tacgcatggt
     1081 atcaccgttt gggtcaactt cgacgttcag atgtccgact ccattgccaa cagaatgatc
     1141 aagaacagat atcagatcat caatcatttt attttcatcc atggtttcct cttttccagt
     1201 gactatttgc tgagttcaaa ttcgatatca cctgcaaaga ctctgtcttc tttaccaagt
     1261 gcgccgggaa tgatcgtaaa tgtgtttgaa ataccatcct gtgcatcatc gaacggaagc
     1321 ttggcaacgg aagacaatga tgcgaccgca ccattggaat cacgcccatg catcggattg
     1381 gcaccaggag caaacggctg tcccatttta cgcccgtccg gagtgttgcc ggtagctttg
     1441 ccataagtta cattagaagt aatcgtgaga acggacattg ttggaaaccc gtctctatag
     1501 gtgtggtgcc ttctgattgc agtcataaag cgtgacacga catgctgggc gatcgtatcg
     1561 actctgtcgt cgtcattgcc gtatttagga aagtcgcctg ttatcctgaa gtcggtaata
     1621 atgccgtctt cgtcccggat cacttctact tttgcatatt tgacagccga taaggagtct
     1681 gctacgatcg aaagacctgc gataccggtt gcgaaatatc ttttgacatc tctgtcgtgg
     1741 agggccatct gtgcccgttc ataacaatat ttgtcgtgca tgtagtgaat aatgttcagc
     1801 gtattcacat agacatcagc ctgccattcc atcatatcca tgaattttgc aagcacatcg
     1861 tcatagtcaa gcacgtcgcc ctcaaccgga cggtactttg gtccgatctg cttctttgtt
     1921 acttcgtcga caccgccgtt taaagcataa agcaggcatt tggccagatt ggcacgtgcg
     1981 ccgaaaaact gcatttcctt gccgatcacc atggaagaga cacagcatgc gataccataa
     2041 tcgtctccat gtgtgattct cataagctca tcattctcat actggatgga agacgtatta
     2101 atggaagttt ttgcacagaa tcttttaaag ttttccggaa gtctcggtga ccagaggacc
     2161 gtcagattcg gttccggcgc agcaccgagg ttattgagcg tatgcaggta gcggaaagac
     2221 attttcgtga caagatgacg tccgtcaata ccaatgccgc cgatcgattc cgttacccag
     2281 accggatctc cggcaaaaat ctgattatat tccggtgtgc gtgcaaattt gatacacctt
     2341 aatttcataa taaagtgatc gacaaattcc tggatctgct cttcggtaaa cgtaccgttt
     2401 cttaaatccc gttccgcata gcagtcgata aaagtagaag tacggccaag agacattgcc
     2461 gcaccgttct gttcttttac tgcgcataaa taaccgaagt atgtagcctg aatagcttcc
     2521 tgtacatttt tggaaggccc ggaaatgtca cagccataag aggcagccat ttctttcatc
     2581 attttaagtg ctgtcatctg ttccgagagt tcttcgcgaa ggcggatgac atcatctgtc
     2641 ataacgcgtc cggtagaaga tctctgcgct tttttatctt cgatcaatct gtcgatccca
     2701 tagagtgcaa ttcgtctata atcgccaatg atacgtccgc gtccgtaggc atcgggaaga
     2761 cccgtgatga tatgggaaga ccgacaggct ctcatttccg tattgtaagc gtcaaagcat
     2821 cctgcattat gtgtttttct gtgtattgaa aaaaattcac tgatttccgg tgctacttca
     2881 tatccattat ccgagcatgc cttttccgcc atgcggatac cgccatatgg ctgcagggaa
     2941 cgcttaaagg gccggtctgt ctgaaatcct acgatcgttt ccttgtcttt gtccagatag
     3001 cccggaccat gggaagtaat agtagatacg atctttgtat ccatatcaag gacgccgcct
     3061 gctgcgcgtt cctgtttctg cagatcaagg acctgtgccc acagatcttt tgtgttctgc
     3121 gtaggtgcgg ctaagaacga atcatctccg tcatatggcg cgtaattttt ttggataaaa
     3181 tctctgacgt tgatttcatt ttcccatttt ccgcctgcaa aaccattcca ttctgacttt
     3241 gttattatca taatattgcc tcctggtata tatgctgaat gagttttccg gtcaaaattc
     3301 aaccggaaat gtctgcttgt tacaactatg atttatgatt ataggttaat cgtgtataca
     3361 cgtcaataga ggaactgttt tttttctcat acaactgcgg ggcgaaaaag aggcattttt
     3421 tcattaagaa aaaaggtctt aaaagagttc agaaagaatt tagaaaacgt ttagaaaaca
     3481 ttttttgtta tggcttgtaa taaaaacaaa aaaatggtat tctgccaatg tgaggttttt
     3541 agtgtttgta aaaatcatat catgaattgt atattaataa agcagagata aaggaggttt
     3601 taatatggca gcaattacaa aggatatgac gatcggtcag gcgataaccg tcaaccagga
     3661 gattattccg gtccttttgg acataggcat gcattgcatc ggatgccctt ccgcacaggg
     3721 ggagaccctt gaggaagcag caatggttca tggaatggat cctgatgaat tgatggaaaa
     3781 aatcagtgaa gctgttgggg aatagtcatc aggcgctcat gcagtataaa taaaaagtgt
     3841 aagccgaccg gccggagccg atggcttaca cttattttat acgttcagca gtttttgcaa
     3901 ggcatcctct ttcaggatca gttcgttatg ctcttcaaaa taagcttctg ctgccggtga
     3961 atatttctct ggggttccat actcagagat catattgcag atcctgttga aatcttcctg
     4021 cgtatggatt gattttgaaa caataaggac tacccgtcct gattttctat cccggtataa
     4081 ggagttgcgg ccgaaataaa aagttttcat gacatgggcc agtcttgcaa cagcatcgat
     4141 atcgttaaag gagtatgcct ttgataattc gtatgcggca gaagtttttt ccagcagttt
     4201 ttcttttgct tcttcaacaa gtcctgtgga agcaggtgtt ctttcctgtt tcagtttttt
     4261 aaataaatca aggacattat cggtatcgtc tcctatagac tgtataactt catcaagtcc
     4321 ggtgtcttct tcgtcatgta cggaaggcgc gaaattagaa aatcttgtat ccaattcctc
     4381 cggatcttct acctttgtaa taataagtac gatacaatcc gcggataacg gaattgcttc
     4441 tatcataagc gggatgtctt ctgcttcaaa accgaattcg tatgctgcct gctgcatcat
     4501 gtcccggaac aggctttttg ccttttccgt accataggct aattcactga tctttaattc
     4561 tctgtctgcc agatcggcct ttgtcaatgt acagcgtatc tgatggtcat ttactttttc
     4621 gagtttcata gtatcacatc ctttctcgtt tgattctttt ttgtttttat cataatatac
     4681 tttctataat atgaagtcaa tagcctttca gcctgtaaat attaaagttt tgttaaagaa
     4741 aaagaaaagc aatgccattt agttaggctt aattcaattt aattttagtg gttttccctt
     4801 gcag
//
LOCUS       DAOSYE010000042         4775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12054_length_4775_cov_3.498517, whole genome shotgun sequence.
ACCESSION   DAOSYE010000042 DAOSYE010000000
VERSION     DAOSYE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12054_length_4775_cov_3.498517"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..575)
                     /locus_tag="PLU43_01315"
     CDS             complement(96..575)
                     /locus_tag="PLU43_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOO27075.1"
                     /translation="MDKPLLYRKRIIPEECIALKDDIILLQNEDIIITKWRALKPKKD
                     LHHGYSCYFLKEGYKISKFYKEDHSLMYWYCDIITHTYSKETNTYVFIDLLADVLIYP
                     DGFVKVVDIDEISEALQTGKITTEETCRTLNSLDRLLKIIYGNQFYKLQKLIEDVED"
     gene            786..1079
                     /locus_tag="PLU43_01320"
     CDS             786..1079
                     /locus_tag="PLU43_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOO27076.1"
                     /translation="MKYKKEVYRSLALITQFGIHMLVPIFLCTFLGIFLDKIFHKSFL
                     VIIFFFIGALAGFRNVFLSAKQIYEDTDVHHEEIPGVDGDERPDDGEETSGGK"
     gene            1048..1461
                     /locus_tag="PLU43_01325"
     CDS             1048..1461
                     /locus_tag="PLU43_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27077.1"
                     /translation="MMVKKLLAENETLRELLAGILISGFSMQLLLVIFTKHFAYYAIG
                     LWTGIAAALIMAVHMAYALDTALSLGEKGAAAYIRKKSIVRYVLVCILLVLAGITNFG
                     SPVTLLLGVMGLKTGAYLQPVTHKMIIKLNRKQGR"
     gene            1470..2192
                     /gene="atpB"
                     /locus_tag="PLU43_01330"
     CDS             1470..2192
                     /gene="atpB"
                     /locus_tag="PLU43_01330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOO27078.1"
                     /translation="MNMCVLLSGDNVDFMIKGLFSYQIFGQTVWITTSHVCILIVMLM
                     LITFCIAANRVIKRANVEDTPSTFQNVVELIVEMLDKMVTGVMGKNAPKFVNYISTIF
                     LFILISNISGIFGLRPPTADYGVTFPLGLITFALIFFNKIKYQKVSGFIKGLCKPWPI
                     WLPINIIGDIAVPISLSLRLFANVLSGVVMLALVYGLLPIFVKIGFPAALHVYFDLFS
                     GGIQTYVFCMLTMTYVTDAIEG"
     gene            2281..2535
                     /gene="atpE"
                     /locus_tag="PLU43_01335"
     CDS             2281..2535
                     /gene="atpE"
                     /locus_tag="PLU43_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOO27079.1"
                     /translation="MQISGEDFILGCSAIGAGLAVIAGIGPGVGQGIAAGHAASAVGR
                     NPGAKSDITSTMLLGQAVAETTGLYGLLIAMLLLFVKPLV"
     gene            2619..3134
                     /gene="atpF"
                     /locus_tag="PLU43_01340"
     CDS             2619..3134
                     /gene="atpF"
                     /locus_tag="PLU43_01340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOO27080.1"
                     /translation="MERLFDLDFQLLHDAVLAAIAVFCLFLIMSYLLFNPVKDLLKRR
                     QEKIQGDIEQAKKNKEDALSLKEQYDTKIRQAEKESEEILAAARKKALTNEAEIVAEA
                     KEEAARIIKQARIEAGLEKQKAADEMKREMIEIASLMAGKVVSASIDTAVQNALVEET
                     LEEVGDQTWLS"
     gene            3122..3676
                     /gene="atpH"
                     /locus_tag="PLU43_01345"
     CDS             3122..3676
                     /gene="atpH"
                     /locus_tag="PLU43_01345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOO27081.1"
                     /translation="MAKLVSNTYGDALFDLSLEETSMDELFSEVLELKEILKENPDFF
                     KLMCHPKIEKNEKEAIMESVFKGRLSDELTGFLKIIINNSRYAEIYSILDYFIARVKE
                     YKKIGIAYVTTPLPLDAEQKTAVEKKLLGTTIYEKMEVNYLIDRSIIGGMVIRIGDRV
                     VDSSVKTKLNTLANELQKIQLARC"
     gene            3729..>4775
                     /gene="atpA"
                     /locus_tag="PLU43_01350"
     CDS             3729..>4775
                     /gene="atpA"
                     /locus_tag="PLU43_01350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOO27082.1"
                     /translation="MNLRPEEISSVIKDQIKRYAGELEVSEVGTVIQIADGIARVHGL
                     EKAMQGELLEFPGEVYGMVMNLEEDNVGTVLLGADRSVGEGDTVKTTGRVVEVPVGDA
                     MLGRVVNSLGQPIDGKGPIQTEKYRQIERVASGVITRKSVDTPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQNVKCIYVAIGQKASTVAAIVKTLEEYGAM
                     AYTTIVASTASEMAPLQYIAPYAGCAIGEEWMENGEDVLVIYDDLSKHATAYRTLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARMSEEYGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLETEMF"
BASE COUNT         1404 a          845 c         1182 g         1344 t
ORIGIN      
        1 cggtcggcaa ctgcctttat ttcagaactg tcataccgca gatcaacgga cagatcatcg
       61 gcggccatgt attttactag aagcgccgga ataatttaat cttcaacgtc ttcgattaat
      121 ttctgaagtt tgtaaaactg attgccataa atgattttca gaagcctgtc aagactgttt
      181 aaggtccggc aggtttcttc tgttgtgatt tttcctgtct gcagggcttc ggatatttcg
      241 tcaatatcga cgacctttac aaaaccgtca ggatagatca gtacatcagc cagaagatcg
      301 atgaaaacat aggtgttggt ttctttggaa taggtgtggg tgatgatgtc gcagtaccag
      361 tacatcaggg aatggtcttc tttatagaac ttgctgattt tgtagccttc tttgagaaaa
      421 taacaggaat agccgtgatg cagatccttc tttggtttta atgcgcgcca ttttgtaatg
      481 atgatatcct cgttctgtaa aagaatgatg tcgtccttta aggcaataca ctcttcggga
      541 ataatgcgtt ttctgtaaag aagcggtttg tccatagaaa tccctccgaa atagaatatt
      601 ttaatttaat ccagtataac aataaatgct gaaaaagtct ataaaatgta tgaaatgtaa
      661 agattatgtc aaaaacagag taaggtacaa aaaaacattg aaaatttgtt gaaaatgtat
      721 attttttcta gacttttaaa aaaaaactgg tataatatta ttattatatt tgaaaggaaa
      781 tttaagtgaa atataaaaaa gaagtctacc gttctcttgc actgatcaca caatttggaa
      841 tccatatgct tgtcccaatt tttctgtgta cgtttctggg aatttttctg gataaaatat
      901 ttcataaatc ctttctggta atcatatttt ttttcatagg tgccttggcc ggattcagaa
      961 atgtgttttt atctgctaag cagatatatg aagatacgga tgtacatcat gaagagattc
     1021 ctggtgtgga tggagatgag agaccggatg atggtgaaga aacttctggc ggaaaatgaa
     1081 acactgagag aactgcttgc cgggattttg atttccgggt tttcgatgca gcttttgctt
     1141 gtaattttta caaagcattt tgcctattat gcaatcgggc tttggaccgg tattgcggcg
     1201 gcacttatta tggcagtgca tatggcatat gcgcttgata cggcactttc ccttggggaa
     1261 aaaggtgcgg ctgcctatat cagaaaaaaa tcaatagtca gatatgtgct tgtgtgtata
     1321 ttgcttgtgc tggccggaat tacgaatttc ggaagtccgg ttactctttt gcttggagtc
     1381 atgggcctga aaacaggcgc ttatctgcag cctgttacgc ataaaatgat tataaaatta
     1441 aacagaaaac aagggaggtg aagaaataaa tgaatatgtg tgttttattg tcgggcgata
     1501 atgtggattt tatgatcaag gggctgtttt cttaccagat attcggtcaa acagtttgga
     1561 tcacgacgag tcatgtctgc attttgatag tcatgctgat gctgatcacg ttttgtattg
     1621 ctgcaaaccg tgtgatcaaa cgggcaaatg tggaggatac gccgtccacc tttcagaatg
     1681 ttgtagagct tattgtggaa atgctcgata aaatggtaac tggtgttatg ggtaaaaatg
     1741 ctccaaaatt cgtaaactat atcagtacga tatttttatt catattaatc agcaatatat
     1801 caggtatttt tggtctccga ccgcctactg ccgattatgg tgtgacattt ccgctgggcc
     1861 tgattacgtt tgctctgata ttctttaaca agatcaaata tcagaaggta agcggcttca
     1921 tcaaaggatt atgtaaacca tggccgatct ggcttccgat caatattatc ggagatatcg
     1981 ccgttccgat ttccctgtcc ctgcgtttgt ttgcaaacgt attgtcagga gtcgttatgc
     2041 tggcgcttgt ttacggactg cttccgattt ttgtaaagat cgggtttccg gctgcgctgc
     2101 atgtttattt tgatttgttt tccggcggga tccagacgta tgtattctgt atgctgacaa
     2161 tgacttatgt gacggatgcg attgagggat aacgcgatat aggatctttt gcaataggaa
     2221 tgaaagaaaa ttccgtaatt acattataat tcaatatacc aattttaagg aggatttgct
     2281 atgcagattt caggagagga ctttatttta ggatgttccg caatcggagc cggactcgcg
     2341 gtgattgcag gtatcggacc tggtgtaggc cagggtattg ccgcaggcca tgctgcttcg
     2401 gcagttggaa gaaatccggg agcaaaatcg gatattacat ctacgatgct tcttggccag
     2461 gccgttgccg agacaacagg actttatggt ttgctgattg caatgctgtt attatttgtt
     2521 aagcctcttg tataggtatt accgcaaata atctgaaagg aggctaaaac ttggagcaaa
     2581 tatattacgc cgtactgttg agcggcgccg atgaaccgat ggaaagactg ttcgatctgg
     2641 attttcagct tcttcatgat gcggtgcttg ctgccatagc ggtattctgt ctgtttctga
     2701 tcatgtctta tctgcttttt aacccggtca aagatctgct gaaaagacgc caggagaaaa
     2761 tacagggcga tattgagcag gcaaagaaaa ataaagaaga cgcgctgtct ctgaaggaac
     2821 agtatgacac aaaaataaga caggcggaaa aagaatcgga agagattctt gctgccgcaa
     2881 gaaagaaagc cctgacgaac gaagcggaaa ttgtggccga ggcaaaggaa gaggcggcac
     2941 ggatcattaa gcaggcacgt attgaagcgg ggctcgaaaa gcaaaaggcc gctgatgaga
     3001 tgaaacggga gatgatcgag atcgcatccc tgatggcagg aaaagttgtt tccgcatcga
     3061 tcgatacagc cgtacagaac gcccttgttg aggaaacatt ggaagaagta ggtgatcaga
     3121 catggctaag ctagtttcaa atacatatgg agatgcttta ttcgatcttt cgctggaaga
     3181 aacatcgatg gatgaacttt tttcagaagt actggaatta aaggagatct taaaggagaa
     3241 tcctgatttt ttcaagctga tgtgtcatcc caagatcgaa aagaatgaaa aagaagcgat
     3301 catggaatcg gtgtttaaag ggagattatc cgatgaactg accggatttc taaagatcat
     3361 aattaacaac agcagatatg cggaaatata ttcaatactg gattacttta ttgcaagagt
     3421 aaaagagtat aaaaaaatcg gaattgctta tgtaacgact ccgctgccgc ttgatgctga
     3481 acagaagaca gcggtggaga aaaaactgct tgggacgacg atttatgaaa aaatggaggt
     3541 caattatcta attgatcgtt ccattatcgg aggcatggtg atccggatcg gagacagggt
     3601 cgttgacagc agtgttaaaa cgaaactgaa tacgcttgcc aatgaactgc aaaaaataca
     3661 gcttgcccgc tgctgatcaa aaagcaggca gattttgttg ataaaatgaa gaaaggtgcg
     3721 taagatccat gaatttgaga ccagaagaaa taagctcggt aataaaggat caaatcaaga
     3781 gatatgccgg tgaacttgag gtatccgaag tcggtacggt tatccagata gcggacggta
     3841 tcgcacgtgt acatggtttg gaaaaggcca tgcagggaga acttctggaa tttcccggtg
     3901 aagtttacgg catggtcatg aatcttgaag aggataacgt aggtacggtc ctgcttggcg
     3961 cagacagaag cgtcggtgag ggggataccg taaaaacgac aggaagggta gtggaagtac
     4021 ctgtcggcga tgccatgtta ggacgtgttg tgaactctct tggacagccg attgacggaa
     4081 agggtcctat ccagacagaa aaataccggc agatagagcg tgttgcatcc ggcgttatta
     4141 caagaaaatc cgttgatacg ccgctgcaga caggaatcaa ggcaattgat tccatgatac
     4201 ctattggaag aggacagaga gagctgatta tcggcgacag acagaccggt aagactgcga
     4261 ttgcaattga tacgatcatt aatcagaagg gacaaaatgt caaatgtatt tatgttgcga
     4321 tcggacagaa agcatctacc gtggcggcga tcgtaaagac gctggaagag tacggagcga
     4381 tggcttatac tacgatcgtg gcatcaaccg caagtgaaat ggcgccgctt cagtatattg
     4441 caccatacgc aggctgtgcg atcggtgaag agtggatgga aaacggagaa gatgtgcttg
     4501 ttatttatga tgatttaagt aagcatgcaa ctgcatatcg tacgctttcc ctgcttttga
     4561 gacgtcctcc cggacgtgag gcatatccgg gtgacgtatt ctatctgcat tccaggcttt
     4621 tggaacgtgc ggccagaatg agcgaggaat acggcggcgg ttccctgact gcgcttccga
     4681 tcattgaaac acaggcagga gatgtttctg cctatattcc gacaaatgtt atttccatta
     4741 cggacggcca gatctatctg gagacagaaa tgttt
//
LOCUS       DAOSYE010000043         4728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12240_length_4728_cov_3.235181, whole genome shotgun sequence.
ACCESSION   DAOSYE010000043 DAOSYE010000000
VERSION     DAOSYE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12240_length_4728_cov_3.235181"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLU43_01355"
     CDS             <1..1257
                     /locus_tag="PLU43_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOO27083.1"
                     /translation="AAQAPEEDEEKEPEEEEPKEQAILVNLSGYETTDSKIAVFRGED
                     MNESFEIIDADTKQSVYAGSIIRAVRNTKAGEIDAYGDFSDFDEPGTYYISTQNHGES
                     ASFVIEKNHYQKLLDERTQYSMQNRQGGILNAAQLNDCMMQVTDWLLTYEFLTEPSED
                     STLPEVISFARQRIELLQSYQDTADGSIHIKDITENTIYYRYAAVFAMFADIYQTFDE
                     AYADTCREQAIAAWNYAEANTEALEPDSCSCDDERYWASAQLYKLTGEKEYRSIAESY
                     VGETIPVGFSDDETGYLGSLAYLTTTNKTELKLSEQIMKAVFDDAIAIVNASAKDGYM
                     IAAGDAYDEDSVLTAFANARLLTFANIISKSVDYVDTAANHLDYLYGRNPYMINYAGD
                     ETSAYYNEPETFILTGMLRSYIVAGQ"
     gene            1377..2408
                     /locus_tag="PLU43_01360"
     CDS             1377..2408
                     /locus_tag="PLU43_01360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO27084.1"
                     /translation="MKIVVLAGGISTERDVSLSSGTQIYKALKANGHQAILLDVFLGY
                     EGDCAHIFEKETDWIQEVAPVGETAPDIAQVKASRKDQSGVLFGENVIVICQEAELVF
                     MALHGDCGENGKIQAAFDLFDIKYTGTDSLSSALAMDKTLTKQLFVMNGIKTPKSYLL
                     AGPKDTTKPEFPCVVKISGGGSSVGVYIAKNEEEYKAAVKEAFCYEGKVLVEQYIKGR
                     EFTACVIEDKALPVVEIAPLSGFYDYKNKYQAGSTIETCPAQIDEALTKKIQNMAVLA
                     YQVLGISTYARMDFMVDENGECYCLEANTLPGMTPTSLIPQEAAAIGKSFAELCEWII
                     EISMKKYEK"
     gene            2431..3843
                     /gene="murF"
                     /locus_tag="PLU43_01365"
     CDS             2431..3843
                     /gene="murF"
                     /locus_tag="PLU43_01365"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOO27085.1"
                     /translation="MKGMTLQKITEACGGELHIHIGKELVPAVLLKEAASVVIDSRKA
                     EPDCIFIATKGERVDGHSFIPQVLKAGALGIVCEQEPAAGTDGCFIVVQDSFQALKDI
                     AQYYRSVLPVKTVGITGSVGKTSTKEMIASVLSKRFFIWKTAGNFNNEVGVPLTIFGI
                     REEHEIAVLEMGISDFGEMDRLSRIVRPDVCVMTNIGPCHLENLKSLDGVLKAKSEIF
                     HFMDPDGTVCINGDDEKLKTIREVNKKPPVCFGRGMENDVYATDLVSHGLEGTSCTIH
                     TKAGVFDVKIPLPGSHMADNALAAAAVGLVFSMTPDEIKRGIESVEGMSGRSHLIRTE
                     NYLLVDDCYNANPKAMQAAIDLLSQAEGRKVAILGDMFELGEDSDQLHAKTGRYAAVH
                     AVDVLLCVGTQSKYMYEAATAIAAGNDMQLYYYESREELLEKLRKEALLQKGDTILIK
                     ASHGMGFEKVVEFLQTYGIK"
     gene            complement(3861..4595)
                     /locus_tag="PLU43_01370"
     CDS             complement(3861..4595)
                     /locus_tag="PLU43_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO27086.1"
                     /translation="MIRFISAVLFVVLFLILSIPLLIAEWIIGKCNMQVKDKSSLAIV
                     NWAFRCVLSISGAKMHIIGEELVPKDRAVLYVGNHRSYFDVIMTYTRVPRPTGYIAKK
                     EMLHYPLLMHWMKNLHCLFLDRTNLKSGMKTILEGIDLIKNGTSICVFPEGTRNKTDQ
                     MLPFKEGSLKMAEKSGCPIVPIAITNSADIFENHFPKMRKTDVLIEYGKPFYPKDLTK
                     EERKFLGSYTQNLIQTMLDDHKKMSQ"
BASE COUNT         1417 a          899 c         1248 g         1164 t
ORIGIN      
        1 gcggcgcaag cgccggaaga agacgaagag aaggagccgg aggaagaaga gccgaaggaa
       61 caggcgattc ttgtcaattt atcaggatat gaaacgaccg acagtaaaat tgccgtattt
      121 cgcggagagg atatgaatga aagttttgag atcatagatg ccgatacaaa acagtctgtt
      181 tatgcaggat ccattattcg ggctgtgaga aatacgaaag caggggaaat cgatgcttac
      241 ggtgatttca gcgactttga cgagccgggg acatattata tcagtacgca gaatcatggg
      301 gaatccgctt catttgtgat cgaaaaaaat cattatcaga agttgctaga tgagagaacg
      361 cagtattcca tgcagaaccg gcagggcgga attttaaatg cagcacagct gaacgattgc
      421 atgatgcagg tcacagactg gcttttgacg tatgaatttt taaccgaacc gtctgaggac
      481 agtacactgc ccgaagtaat ttcctttgcc agacagagga tcgagctgct gcagtcatat
      541 caggatactg cagacggcag cattcacatc aaggatataa cggaaaatac gatctattat
      601 cgttatgcgg ctgtatttgc aatgtttgcg gatatctatc agacatttga tgaagcatat
      661 gccgacacat gccgggaaca ggcgattgcc gcatggaact atgcggaagc aaataccgaa
      721 gccctggaac cggattcctg cagctgtgat gacgaacgtt actgggcgtc cgcccagctt
      781 tataagctga caggagaaaa ggaatatcgt tctatagcgg aaagctatgt cggggaaacg
      841 ataccggtcg gattttccga cgatgaaacc ggctacctgg gatcgcttgc ctatctgaca
      901 acaacaaata aaaccgaact gaagctgagc gaacagatca tgaaggcggt atttgacgat
      961 gcgatcgcga tcgtaaatgc ttccgcaaaa gacggttata tgattgctgc cggggatgca
     1021 tatgatgagg acagtgtgtt aactgccttt gcaaatgcgc ggctcctgac gtttgccaat
     1081 attatcagta aaagcgtcga ttatgtggat acggcagcga atcatctgga ctatttgtat
     1141 ggcagaaatc cttatatgat caattatgcg ggcgatgaaa catctgctta ttacaatgaa
     1201 ccggaaacat tcatcttaac agggatgctc cgttcttata ttgtagcagg acagtagtca
     1261 gacagcagat tttacaatag ttttgacagc ttttacaata ggtatgaaaa acggaccgga
     1321 ttactatata taagaaagta tttttagaaa aatcgcggaa gggaaaaggc ttgcatatga
     1381 aaatcgtagt tttggcagga ggtatcagta cggaaaggga tgtgtcctta agctcaggga
     1441 cgcagatcta taaagcattg aaggcaaacg ggcatcaggc gatcctgctt gatgtatttt
     1501 taggatatga aggggactgt gcgcatattt ttgagaaaga gaccgattgg atacaggaag
     1561 ttgcgccggt cggagaaacc gcgccggata tcgcgcaggt aaaagcatcc agaaaagatc
     1621 agtcaggggt cctgtttgga gaaaatgtga ttgtgatctg tcaggaggca gagcttgtat
     1681 ttatggcact gcacggagac tgcggagaaa acggaaaaat acaggcagcg tttgatctgt
     1741 tcgatattaa atatacggga acggattctt taagcagtgc acttgcaatg gataaaaccc
     1801 tgacaaaaca gctgtttgtc atgaatggga taaaaacgcc gaaaagctat ctgcttgccg
     1861 gaccaaagga tacgacaaaa ccggaattcc cgtgtgttgt gaagatcagc ggcggcggtt
     1921 caagcgtggg cgtttatatt gcaaagaacg aggaggaata caaggctgcc gtgaaagaag
     1981 cattttgcta tgaagggaaa gtccttgtgg aacagtatat caaaggacgc gaatttacag
     2041 cctgtgttat tgaagacaaa gcgcttccgg tcgtggaaat agctccgctc agcggatttt
     2101 atgactataa gaacaaatat caggcaggca gtacgatcga aacttgtcct gcgcagatag
     2161 acgaagcgct gacaaagaaa atacagaata tggcggtgct tgcctatcag gtgcttggaa
     2221 tcagcacgta tgcaagaatg gattttatgg tagatgaaaa tggggaatgc tattgtctgg
     2281 aagccaatac gctgcccggg atgacaccga cgagcctgat tccgcaggaa gcggccgcga
     2341 tcgggaaatc attcgcagag ctgtgtgagt ggatcatcga gatttccatg aaaaaatatg
     2401 aaaaataaca ataacaggac ggaagatact atgaagggaa tgaccctgca gaaaattacg
     2461 gaagcgtgcg gcggagaact gcatattcat atcgggaaag agcttgtgcc ggctgttctt
     2521 ttgaaagaag cggcatcggt cgtgatcgat tcgagaaagg cagagccgga ttgtatattt
     2581 attgcaacaa aaggggagcg tgtggacgga cattctttta ttccgcaggt tcttaaagca
     2641 ggcgcacttg gaatcgtctg tgaacaggag ccggctgctg gaacagacgg ctgctttatt
     2701 gttgtacaag acagttttca ggcactgaag gatatcgcac aatattaccg cagtgtcctg
     2761 cctgtaaaaa cagtagggat tacgggaagt gtcggcaaga caagtacgaa ggaaatgatc
     2821 gcatccgtac tttccaaacg gtttttcata tggaagactg caggaaattt taataatgaa
     2881 gtaggcgttc ctctgacgat ttttggaatc agagaagagc atgagatcgc agtattggaa
     2941 atggggatca gtgattttgg tgaaatggac aggctaagca ggatcgtaag gcctgatgtc
     3001 tgtgtaatga cgaacatcgg cccgtgtcat ctggaaaatc tgaaatcgct ggacggcgta
     3061 ctgaaagcaa aatccgaaat tttccatttt atggatccgg acggaacggt ctgtattaac
     3121 ggagacgatg agaaactgaa gacgatccgt gaagtaaata aaaaaccgcc ggtctgtttt
     3181 ggaagaggaa tggaaaatga tgtttatgcg acggatcttg tgagtcatgg tctggaggga
     3241 acgtcctgta cgatccatac aaaggcaggc gtatttgatg tgaagatccc gcttcccggc
     3301 agccatatgg cagataatgc gcttgctgcc gctgcagttg gtctggtctt ttccatgacg
     3361 ccggatgaaa taaaacgtgg gatcgaatca gtggaaggca tgagcggaag aagccatttg
     3421 atcagaacag aaaactatct gcttgtagat gactgttata atgcgaatcc aaaagccatg
     3481 caggcggcga tcgatctgct ttcccaggca gaaggaagaa aggtcgccat tcttggagat
     3541 atgtttgagc ttggagaaga cagcgatcag ctgcatgcta aaacaggccg ctatgcggcg
     3601 gtacatgcgg tggatgtact gctctgtgtt ggcacgcagt caaaatatat gtatgaagcg
     3661 gccacagcca tagccgcagg aaatgacatg cagctgtatt attatgagtc gagggaagag
     3721 ctgcttgaaa aactgcggaa agaagccctg ctgcagaagg gggatacgat cctgatcaag
     3781 gcatcacacg gaatgggatt tgaaaaggtc gtggaatttt tgcagacata tggaatcaaa
     3841 taaaaaaaag atgtctcgga ttattgagac atctttttat gatcatccag catcgtctga
     3901 ataagattct gtgtgtagct tccaaggaat ttgcgttctt cctttgtaag atctttcgga
     3961 taaaacggct ttccgtattc gatcaggaca tcggttttcc gcattttcgg aaaatggttt
     4021 tcaaagatgt ccgcagaatt cgtgattgcg atcggtacga tcgggcagcc tgatttttca
     4081 gccattttca gacttccttc tttgaacgga agcatctggt cggtcttgtt gcgcgtgcct
     4141 tccggaaata cgcagatgga cgttccgttt ttgatcaggt cgatcccttc cagaattgtt
     4201 ttcatacctg atttcagatt ggtcctgtca agaaaaagac aatgcagatt tttcatccag
     4261 tgcatcagca gagggtagtg cagcatttct tttttggcaa tatatccggt cggtctcggc
     4321 actctggtgt aggtcataat aacatcaaaa tagcttctat ggtttccgac ataaagtacg
     4381 gcgcggtctt ttggaacaag ctcttccccg atgatatgca ttttggcacc ggaaatagac
     4441 agcacacagc gaaaggccca gtttacgata gcaagggaac tcttgtcctt tacctgcata
     4501 ttgcatttac cgatgatcca ttccgcaatc agcaggggga tgctcagtat aagaaacaga
     4561 acaacaaata atacggcaga aataaaacga atcatggcag tttgtcctcc aggtacgttt
     4621 ctattatagt aaaagaacag ggaaaacaca agaacgtctt cattgtattt tgccgtaata
     4681 tccaatataa taaaaaccag aggacattta gttaatttcg agggagga
//
LOCUS       DAOSYE010000044         4705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12328_length_4705_cov_3.764516, whole genome shotgun sequence.
ACCESSION   DAOSYE010000044 DAOSYE010000000
VERSION     DAOSYE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12328_length_4705_cov_3.764516"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLU43_01375"
     CDS             <1..237
                     /locus_tag="PLU43_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO27087.1"
                     /translation="VAAMFMSACSTSNAFIGKSFSNNFAAVPILAFIVTGPMLDLKNM
                     IMLSEILKKRFLLLLAALVILESALVFGTLSFFM"
     gene            248..1162
                     /locus_tag="PLU43_01380"
     CDS             248..1162
                     /locus_tag="PLU43_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HOO27088.1"
                     /translation="MGRNKNLEAVIQICLLLMIACLLFAVMVSGKISNYIHPRFYFGL
                     WISIIILLLFAGSLFSKIKKARHNVNIRHYFIFMIPIAAALLFPAAGVNNKDMTVAAG
                     SAAGETGQQTESLAGSGQTSSDYTESSLFGNTGANYGASDDLSSSAAADTSSWDTQET
                     AAADDAKEETISMSEKYAPYETDGILYITDDIFADWYCDVYDYLDEFVGKKCSYLAQV
                     YSMDDFKEDQFLAGRYFMVCCAADLVGYGIICESDRRGELTDDEWITVTGTIASCEYN
                     GYMVPLLTDVTITDGEEPEVAYIYYNSY"
     gene            1276..1590
                     /locus_tag="PLU43_01385"
     CDS             1276..1590
                     /locus_tag="PLU43_01385"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOO27089.1"
                     /translation="MPRRDGTGPAGMGAMTGRGAGYCNKDARNIDFGRSFGGYGCQRG
                     YRRQFVQTGVPGYLRDDAMQYEQGTDTKEFWETQEAGLKNRLQFVQERLAGMKKPAAP
                     QE"
     gene            1711..2565
                     /locus_tag="PLU43_01390"
     CDS             1711..2565
                     /locus_tag="PLU43_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOO27090.1"
                     /translation="MELFYDCIPCMIRQALEAARKTTDDVAIHDKVVTETLCLMKDYK
                     KYRNSPDLAREIHRILKEQTGLYDPYLKIKEKDLNAAKSVYSYLKEFMHEKGDDLYWA
                     LKVAATGNNLDAAIYDEVDLEKCIEKEMEKEFSICDLALMEKKLESASNILIIGDNTG
                     ETVFDKVMIECFPKINITYAVRSEPIINDATKEEAVASGLDEVARIVSTGCNAPGAIL
                     EDCSDEFIDIYHKSDIIISKGQGNYEALSDEEGNLFFLLKAKCPMIARRLQVDLNAYV
                     LRYGGIKE"
     gene            2584..3063
                     /locus_tag="PLU43_01395"
     CDS             2584..3063
                     /locus_tag="PLU43_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOO27091.1"
                     /translation="MARPKKWRRIEHIPAIPYFVPSETNTEGLEENILQLEELEAIRL
                     KDLEGLEQGECAERMEVSRPTFQRILLSAREKLADSLINGKMLHIEGGNFTRNICPIR
                     CDDCGYEWKESFENLEAIKNGTYACPNCGSVNITCGHNCRDKFCHKNCHRHRGADFK"
     gene            3105..3596
                     /locus_tag="PLU43_01400"
     CDS             3105..3596
                     /locus_tag="PLU43_01400"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO27092.1"
                     /translation="MQKKEAKKKGALQPAPKVLVSCRGLDGEDNALAVGYCGNCSYAP
                     PMVMVGIVPTRYSYHMIKESGCFVVNIADKAYQETFDYLGSVSKKDSDKLKDKNVRLE
                     DGKVVNAPILADCPVNIECTVVDSIMTGSHEMFVGKVEYVHANADITDAEGNIDFSLI
                     DYI"
     gene            3844..4689
                     /locus_tag="PLU43_01405"
     CDS             3844..4689
                     /locus_tag="PLU43_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOO27093.1"
                     /translation="MNEKEKGCADSSCTKESCEGCEHSRQSMLEELNPYSSVKNVIGV
                     VSGKGGVGKSFVTAMLAVLLNRKGYNVGILDADITGPSIPKMFGINRKAMGNEQGVIP
                     ESTKGNIKIMSLNLLLDKEDAPVIWRGPILANTVKQFWTEVVWGDLDYLFIDMPPGTG
                     DVPLTVFQSIPLDGIVIVTSPQDLVSMIVKKAYHMAEMMDIPVLGLVENMSYVKCPDC
                     GKKISIFGESKLESISEEIGIDILGRIPIDPAIAGLVDAGDFESFSCDCLSEAAEKLS
                     HSDAS"
BASE COUNT         1435 a          836 c         1265 g         1169 t
ORIGIN      
        1 gtggcggcaa tgtttatgtc cgcctgttcg acatccaatg catttatcgg aaaaagcttt
       61 tcaaataatt ttgcagcggt tccgatcctg gcgtttattg taacaggacc gatgcttgat
      121 ttaaaaaaca tgatcatgct ttcggaaatt ctgaagaagc gctttctgct gcttctggca
      181 gcacttgtaa ttcttgaaag tgcacttgtg ttcggaacgc tgtcgttttt tatgtaagga
      241 ggcacgtatg ggcagaaata aaaatctgga agcggtcata cagatatgcc tgctgcttat
      301 gatcgcctgt ctgctgtttg ctgtaatggt cagcggaaaa ataagcaatt atatccatcc
      361 gagattttat tttggattat ggatttctat tataatactg ctgctgttcg cgggaagtct
      421 tttctcaaaa ataaaaaaag caaggcataa tgtaaacatc aggcactatt ttatttttat
      481 gattccgatc gccgccgcac tgttatttcc ggcggcaggc gtaaataaca aagacatgac
      541 tgtggcagcc ggctctgctg caggggagac cggacagcag acggagtctt tggcagggtc
      601 gggtcagaca tcgtccgatt atacggagtc ctccctgttt ggaaatacag gtgcaaatta
      661 cggcgcgtcg gatgacttat cttcttccgc tgctgcggat acctcatcgt gggatacaca
      721 ggaaacagct gctgcagatg acgcaaaaga ggaaacgatc agcatgtcgg aaaaatatgc
      781 gccgtatgag accgacggga tcctttatat taccgacgac atatttgccg actggtattg
      841 tgatgtttat gattatctgg acgagtttgt cggaaaaaag tgcagttatc tggcgcaggt
      901 atattccatg gatgatttta aagaagatca atttttggcg ggcagatatt tcatggtctg
      961 ctgtgctgcc gatcttgtcg gatacggaat catctgtgaa agcgacagga gaggcgaact
     1021 gacggatgac gagtggatca cggtgacagg aacgatcgct tcctgcgaat ataacggcta
     1081 tatggtgccg ctgcttacgg atgtgacaat aaccgacgga gaagagccgg aggtcgccta
     1141 catttattat aacagctact gacagaaaac ttttatttta ttattgacat atgcccaaaa
     1201 tgattgtatg ataattatga gcatatgcta attattaacc tgaaagtaag cagatataag
     1261 aaatggggtg aaattatgcc aagaagagat ggaacaggtc cggctggaat gggagccatg
     1321 acaggacgag gagcgggata ttgcaataaa gatgcccgta atattgactt tggaagaagc
     1381 ttcggcggat acggctgtca gagaggatac agaagacagt ttgtacagac gggagtgccg
     1441 ggatacttaa gagatgatgc catgcagtat gagcagggca cggatacaaa ggaattctgg
     1501 gaaacacagg aagcgggtct taaaaatcgg ctgcagttcg tacaggagcg gcttgccgga
     1561 atgaaaaagc ctgcggcacc gcaggagtag aaatgccgta tatcgtaaat gggaagcctt
     1621 gctgcaaaga cttcccattt acgacggcaa cagcagcaca ggaaatgttt gtgctataaa
     1681 aatttataaa attttgaaac ggagaatgaa atggaattat tttatgattg tatcccctgt
     1741 atgataagac aggcgctgga agctgccaga aagacgaccg atgatgtagc cattcacgat
     1801 aaggtagtga cagaaacgct ttgtctgatg aaggattaca agaaatatag aaattctccg
     1861 gatctggcac gggagatcca cagaatctta aaagaacaga caggactgta tgatccctat
     1921 ctgaaaataa aagagaagga tctgaacgca gcgaaaagtg tctattctta tttgaaggaa
     1981 tttatgcatg aaaaaggaga cgatctttac tgggcactca aggttgcggc aacaggaaat
     2041 aatctggatg cagcgatcta tgatgaggtc gatctggaaa agtgtataga aaaagagatg
     2101 gaaaaagaat tttccatctg tgatcttgcc cttatggaga agaagcttga gagcgcgtcc
     2161 aatatactga tcatcgggga taataccgga gaaactgtgt tcgataaggt aatgatcgaa
     2221 tgttttccaa agataaatat tacgtatgcg gtaaggagcg aaccgatcat caatgatgcg
     2281 acaaaagagg aggcggtcgc ttccggactg gatgaagttg caaggatcgt atctacggga
     2341 tgcaatgcgc cgggggccat tttggaggat tgcagcgacg agtttattga catttaccat
     2401 aaatcagata ttattataag taaaggtcag ggaaattatg aagcactttc ggatgaagag
     2461 ggaaatttgt ttttcctttt aaaggctaaa tgtccgatga tagcacgaag acttcaggtg
     2521 gatctgaatg cctatgtatt gcggtatggt ggtataaaag aatagtacag acagggagat
     2581 catatggcga gaccaaaaaa atggagaaga atcgaacata taccggcaat tccttatttt
     2641 gtaccgtccg aaacgaatac ggagggtttg gaggaaaata ttctgcagct ggaagagctg
     2701 gaagcgatcc ggttaaaaga cctggaagga cttgagcagg gcgaatgcgc ggaaagaatg
     2761 gaagtatcaa gaccgacttt tcagcgtatt ttattgtcgg caagggaaaa gctggcggac
     2821 agcctgatca acggaaagat gctgcatatt gagggcggaa attttacgcg caatatctgt
     2881 ccgatcagat gcgacgactg cggatacgaa tggaaggaaa gctttgaaaa cctggaagcg
     2941 atcaagaacg gaacgtatgc atgtcctaac tgcggttcag ttaatattac atgcggacat
     3001 aactgcaggg ataagttctg ccacaaaaac tgccatcgtc acagaggagc ggattttaaa
     3061 taaagcaggc agcaataaaa ttattatata aaggagtaat gatcatgcag aaaaaagaag
     3121 cgaagaaaaa aggtgcactg cagccggcgc cgaaggtttt agtatcatgc cgcggactgg
     3181 acggagaaga taacgcgctg gcggtcggtt attgcggaaa ttgcagttat gcgccgccta
     3241 tggttatggt cggtatcgtt ccgacaagat attcgtatca tatgatcaag gaatcgggct
     3301 gttttgttgt aaacatagca gacaaggcgt atcaggaaac atttgattat cttgggagtg
     3361 tcagcaaaaa ggattcggat aagctgaagg acaaaaatgt aagactggaa gacggcaaag
     3421 tcgtgaatgc gccgattttg gcagactgtc ccgtcaacat tgaatgtacg gtcgtagatt
     3481 cgatcatgac cggctcgcat gagatgtttg tcggaaaagt agaatatgta catgcgaacg
     3541 cggacataac ggatgcggaa ggaaatatcg acttttcatt gatcgactac atataatgat
     3601 accagggtca aaaggtcatg ttttttatgc ttgcatgaaa aagcatgact ttgagacccg
     3661 caaaaaatga gaaagcagcc cgaaaagggc gaatttcgaa tgtttttagg gttgcggaag
     3721 cacaaagtgc gcagcaaccc gtagtatcaa gggtcggtat ccagggtcgg tatcaagggt
     3781 cagatctatt ttgccccttt cattgtatag atagtaaaca ggataacaga atggagaaaa
     3841 gaaatgaacg aaaaagaaaa aggatgtgct gacagcagtt gtacaaagga gtcatgtgag
     3901 ggctgtgagc acagccggca gagtatgctt gaggagctga atccgtacag ctccgttaaa
     3961 aatgtgatcg gagtcgtaag cggcaaaggc ggagtcggaa agtcatttgt cacggccatg
     4021 ctggcagtgc tgctgaacag aaagggctat aatgtcggta ttctggatgc cgatattaca
     4081 ggaccgtcga ttccgaaaat gtttgggatc aatcgaaagg caatgggaaa tgaacagggc
     4141 gtgataccgg aatccacaaa gggcaatatc aagatcatgt cgctgaatct tctgcttgat
     4201 aaagaagatg cccccgtaat ctggagggga ccgatcttag ccaatacggt caaacagttc
     4261 tggacggaag tcgtatgggg agaccttgac tacttattta tcgatatgcc gccaggtacg
     4321 ggtgatgttc cgcttaccgt atttcagtcg attccgcttg atgggatcgt gatcgtgaca
     4381 tctccgcagg atcttgtttc gatgatcgta aaaaaggcat atcatatggc agaaatgatg
     4441 gatattcctg ttttgggcct tgttgaaaat atgagttatg tgaaatgtcc ggattgcgga
     4501 aaaaagatct ctatatttgg agagagcaag ctggaaagca tttcggaaga gatcggaatc
     4561 gatattttag gcaggatccc gatcgatccg gcgattgccg gactggtcga tgcgggtgat
     4621 tttgagagct tttcgtgtga ctgtctgtcg gaagctgcgg aaaaactcag tcattcggat
     4681 gcttcataga ggctgaccat agctt
//
LOCUS       DAOSYE010000045         4697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12363_length_4697_cov_3.168462, whole genome shotgun sequence.
ACCESSION   DAOSYE010000045 DAOSYE010000000
VERSION     DAOSYE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12363_length_4697_cov_3.168462"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLU43_01410"
     CDS             complement(<1..844)
                     /locus_tag="PLU43_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOO27094.1"
                     /translation="MRKKLKVLVVDDSYVFRKMLSAGLSSDPAIESVITAEDPFDARD
                     KIIEFQPDVMTCDVEMPKMNGIEFIRKLLPQYMLPVVVVSSVSKAVLDAMNAGAVDFV
                     VKPEVQTVKDVKAFIDEVVLKVKIASTAKVCIGTPDSHFHGVQVPIKPSGLNEKEYDR
                     KLIAIGASTGGTEAIYSILMKLPSKCPGIVIVQHIPPVFSKMFADRLNDSTQFSVKEA
                     EDGDFVESGKVLVAPGDRHLKIVKIGERYRTECFKGERINGHCPSVDILFDSVAVSAG
                     ANAMG"
     gene            complement(849..1664)
                     /locus_tag="PLU43_01415"
     CDS             complement(849..1664)
                     /locus_tag="PLU43_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOO27095.1"
                     /translation="MQQITEKEFEKLSEYIKKNYGIFLKKEKMSLVMSRLSGILSEKN
                     MSSFSEYYQYVVSDRTGLAAAELVNKITTNHTFFMREPEHFTYFRDVVLPYLSQNVKD
                     KDLRIWSAGCSSGEEPYTLAMIMNDYFGSEKFIWDTKILATDISGQVLESAKNGIYSM
                     EKVAQLSEMWKRNYFHPYGQETYQISQKMRDEIIFRRLNLNDREFPFRRKFHVIFCRN
                     VMIYFDSETKRQVVEKFYDHMENGGFLFIGHSESLNQDKMKYKYIMPALYRKE"
     gene            complement(1682..3517)
                     /locus_tag="PLU43_01420"
     CDS             complement(1682..3517)
                     /locus_tag="PLU43_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27096.1"
                     /translation="MKKLYDMNIGVKLVIGFVIVALIAGVVGVLGIYFSRTINQADKQ
                     LYEDNTIGLETAANISVCYQRVRFNTIKMLLDELLVENAIEKIEGYREETEAYLLSYA
                     EKISSEEDRQLYEELASTWDIYTGYVDQISKYASSGDTEAGKDLAIGEAATAAENLRI
                     AADNLIEYNSHSAESKSESNDVLAQKALYVMIIIVVIGMILAVVLGMIISRMISVPVN
                     KMKEAANQLALGNVDINLNVDTKDEIGNLAESMRLMIANIREQAMAAEKIAQGDLTAD
                     IRVRSDKDILGQKLYELVEKNNMVLSDIAMASDQVAAGSKQVSDSSIALSQGATEQAS
                     SIEQLTASLEEISSQTKLNADNASEANTLAEVAKDTAIEGNGQMKEMQNAMDEINASS
                     ASISKIIKVIDEIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRNLAARSANAAKE
                     TTDLIEGSIQKVDAGTKIANSTAEALNKIVEDVAKAANLVANIANASNEQATAISQVN
                     QGIMQVSQVVQTNSATAEESASASEELSSQAALLKESVAKYKLKAGKKSGDRFNDLSP
                     EVLSMIKEMENKSSNGHKSNHENEKFGGAGKRQIILNDSEFGKYS"
     gene            complement(3555..4055)
                     /locus_tag="PLU43_01425"
     CDS             complement(3555..4055)
                     /locus_tag="PLU43_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO27097.1"
                     /translation="MAEIMETEMELEIEEDTQKGRFLTFALEKETYGIEIRYVTEIIG
                     IQAITELPELPDYIKGIINLRGKIIPVMDVRLRFKKIPRDYNDRTCIIVIEINEVSIG
                     LIVDSVSEVLTINEQDIIEPPKLNNGQQNRYIKSIGKVGNEVKLLLDCNKLLTENEFQ
                     ELDEVS"
     gene            complement(4063..>4697)
                     /locus_tag="PLU43_01430"
     CDS             complement(4063..>4697)
                     /locus_tag="PLU43_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO27098.1"
                     /translation="NPDEMQDKEIFNLIFAPGFSTKEVVSEYSGRGVGLDVVTKNVES
                     IGGSISIDSIGGKGTVITLKIPLTLAIIDGVNVKVGGAYYTIPTISIKEFFRPDEKDI
                     ITDTDGEEMIMIRGKCYPIIKLHEMYQVKGAKTQYEEGIIIMVEQDGSAVCIFADELL
                     GQQQVVVKALPEYVSLIKKIKGLSGCTLLGNGSISLIIDVLGLINSKRER"
BASE COUNT         1154 a         1245 c          814 g         1484 t
ORIGIN      
        1 ctcccatcgc gtttgcaccg gccgaaacag cgaccgaatc aaacagaata tcgaccgacg
       61 gacaatgccc gtttatccgc tcacccttaa agcattctgt tctgtacctt tccccgatct
      121 tcactatttt aagatgcctg tcaccgggtg ccacgagcac ctttccgctt tcgacaaaat
      181 ccccgtcttc tgcttccttg accgaaaact gggtggaatc atttaaccgg tccgcaaaca
      241 ttttcgaaaa cacgggcgga atatgctgta cgatcacgat cccgggacat ttgctcggaa
      301 gcttcatcag aatactgtag atcgcctccg ttccgcctgt tgacgcaccg atcgcaatca
      361 gcttccggtc atattctttt tcattgagcc cgcttggctt tattggtacc tgcacgccgt
      421 gaaaatgtga atccggagtg cctatgcata cctttgccgt tgacgcgatc tttaccttca
      481 gaacgacctc atcaataaac gccttgacat ctttgaccgt ctgcacctcc ggtttgacga
      541 caaaatccac tgctcccgca ttcatcgcat ccaataccgc cttgcttacg gaacttacaa
      601 caactaccgg aagcatatac tgcggcagca gtttacgaat aaattcaatg ccgttcattt
      661 tcggcatctc cacatcacag gtcattacat caggctgaaa ctcgataatt ttatctctgg
      721 catcaaacgg atcttccgca gttataacag attcaatcgc aggatcagag gaaagaccgg
      781 ccgataacat ttttcgaaat acataactgt catccacaac caatactttt aatttttttc
      841 gcattgtttt attccttcct gtagagtgcc ggcataatgt atttatattt cattttatcc
      901 tgattcagtg attccgaatg gccgataaat aaaaatccac cattttccat atgatcataa
      961 aacttttcaa ccacttgcct ttttgtctca ctgtcaaaat aaatcatgac atttctgcag
     1021 aaaatcacat ggaattttcg tctgaacggg aactccctgt cattcaggtt aagacgcctg
     1081 aatatgattt catccctcat tttctgactg atctgatacg tttcctgccc gtaaggatga
     1141 aaataatttc ttttccacat ttccgaaagc tgcgcaactt tttccatact gtaaatacca
     1201 tttttagcgc tttccaaaac ctgtccgctg atatccgtag ccagtatttt tgtatcccag
     1261 atgaattttt ccgatccaaa ataatcgttc atgatcatgg caagcgtgta aggttcttct
     1321 ccgctggaac agcccgcgct ccagatccgc aggtctttgt cctttacgtt ctgcgacaga
     1381 tacggaagaa cgacatctct aaaataggta aagtgctccg gctccctcat aaaaaacgta
     1441 tgatttgtcg ttattttatt gaccagctcg gctgcggcaa ggccggtccg gtcagataca
     1501 acatactgat aatactccga aaagctgctc atgttctttt cagaaagaat accgctcagt
     1561 ctcgacatca ctaatgacat cttttctttt ttcagaaaaa ttccataatt ttttttaatg
     1621 tactccgaga gcttttcgaa ctctttttcg gtaatctgct gcatatgact cccccgcttt
     1681 attatgaata ttttccaaat tcggaatcat tcagaatgat ctgacgtttt cctgcaccac
     1741 cgaatttttc gttttcgtga ttgctcttat gtccatttga tgacttgttt tccatttcct
     1801 ttatcatcga taatacttcc ggactgaggt cattaaatct gtcgcctgat ttcttgcctg
     1861 cttttaattt atattttgct acagactctt tcagcaatgc cgcctggctg gacagctcct
     1921 cgcttgcgga cgcactttcc tccgctgttg cggaatttgt ctgaacgacc tgtgatacct
     1981 gcatgatccc ctgattgacc tgcgagatag cagtcgcctg ctcattcgat gcatttgcta
     2041 tatttgccac aagattcgct gctttggcaa catcctccac gatcttgttc aatgcttcag
     2101 ccgtactgtt agcgatcttt gtaccggcgt ctaccttctg aatagagcct tcgatcagat
     2161 ccgtcgtctc ttttgcagca ttggccgatc ttgcggcaag gttccgcact tcctctgcca
     2221 caaccgcaaa gcctttccca tgctggcctg cccttgctgc ctctacggcc gcattcaatg
     2281 caagaatatt tgtctggaaa gcgatttcat caatgacttt tatgatcttg gaaatgcttg
     2341 ccgaagacgc attgatctca tccattgcat tctgcatttc cttcatctgt ccgttccctt
     2401 cgatcgccgt atccttggct acttccgcca aagtgttcgc ttcgctcgca ttatcggcat
     2461 tcagctttgt ttgagaagag atctcttcca aagaggctgt cagctgttcg atcgagctcg
     2521 cctgctctgt cgcgccctgt gaaagcgcaa tactggaatc cgaaacctgt ttggaaccgg
     2581 ccgctacctg atccgacgcc attgcgatat ccgacaatac catattattc ttttctacca
     2641 gttcatataa tttctgacca agaatatcct tatcggaacg gacacgaata tctgccgtca
     2701 gatcgccctg tgcgatcttt tcggctgcca ttgcctgttc cctgatattt gcgatcatca
     2761 gtctcatgga ttctgcaaga ttaccgatct catcttttgt atcaacattc agattgatat
     2821 ccacattccc aagtgccagc tgattggctg cttccttcat cttatttacc ggcacgctga
     2881 tcattctgga aatgatcatg ccaagcacta ctgcaaggat catgccgatc actacaatga
     2941 tgatcataac atacaatgct ttttgtgcca gcacatcatt gctttcagac tttgattccg
     3001 cactgtgaga attatattcg atcaggttat ccgctgcgat ccttaaattt tcagcagccg
     3061 ttgcagcctc tccgatcgca agatcctttc cagcctctgt gtctccgctg ctggcatatt
     3121 ttgagatctg atcaacataa cccgtataaa tatcccatgt tgaagctaat tcctcatata
     3181 actgccggtc ttcctcagaa gatatttttt ctgcatagga aagcagatat gcttccgttt
     3241 cctctctgta accttcgatt ttttcaatgg cattctctac cagcaattca tcaagcagca
     3301 tcttgatcgt attgaaacga accctctgat agcagactga aatatttgcg gcggtctcca
     3361 gtccgatcgt attatcctca tacagctgtt tatccgcctg attgatcgtt cttgaaaagt
     3421 aaatgcctaa aactcctact acacctgcga tcaaagcaac aattacaaac cctatcacta
     3481 attttacacc gatattcata tcataaagct tcttcatcat cgtttcctcc tagtaaaatt
     3541 tgtatttaca gttactatga cacttcatcc agctcctgaa attcattttc ggtcagcaat
     3601 ttattgcagt ccagaagcag ctttacttca tttccaactt ttccgatact cttgatataa
     3661 cggttctgct gtccgttatt caattttgga ggctcaatga tatcctgctc gtttatcgtc
     3721 agcacctctg aaacactgtc aacgatcaga cctatggaga cctcattgat ttcaattaca
     3781 ataatgcatg ttctgtcatt atagtctctg gggatttttt tgaaacgcag ccgcacatcc
     3841 atgaccggga tgatcttgcc tcgcagatta atgatcccct ttatataatc cggaagctca
     3901 ggaagttctg tgatcgcctg aattccaatg atctctgtta catacctgat ctctatccca
     3961 tacgtctctt tttccaatgc aaatgttaaa aatcttcctt tttgcgtatc ttcttctatt
     4021 tccaactcca tttcagtttc cattatctcg gccatttgtt catcaccttt cccttttact
     4081 gttaataagc ccaagtacat ctatgatcag acttatactt ccgttcccaa gtaacgtaca
     4141 tccggacaga cctttgatct ttttgataag actgacatat tccggcaatg cctttaccac
     4201 gacctgctgc tgtccaagca gctcatccgc aaaaatgcag actgcggaac catcctgctc
     4261 gaccataatg atgatccctt cttcatattg cgttttggcg cctttgacct gatacatttc
     4321 atgaagcttg atgatcggat agcatttgcc ccggatcatg atcatctctt ccccgtccgt
     4381 gtctgtaatg atatccttct catcaggtct gaaaaactcc ttaatagata tggtaggtat
     4441 cgtataataa gcgcctccga ccttaacgtt cactccgtcg ataatagcca gtgtcaacgg
     4501 tattttcaac gtgatcacag ttccctttcc accaatgctg tcaatggaga tggaaccgcc
     4561 gatcgactcc acatttttag ttacaacatc cagtccgaca ccacgtccgg aatattccga
     4621 gactacttcc ttggtggaaa atccgggagc aaaaatcaaa ttaaatatct ccttatcctg
     4681 catttcatcc ggattct
//
LOCUS       DAOSYE010000046         4682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12424_length_4682_cov_3.008429, whole genome shotgun sequence.
ACCESSION   DAOSYE010000046 DAOSYE010000000
VERSION     DAOSYE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12424_length_4682_cov_3.008429"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..386
                     /locus_tag="PLU43_01435"
     CDS             9..386
                     /locus_tag="PLU43_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOO27099.1"
                     /translation="MTNGKKINNRRIGTDHETLAAAYLEKQGVTIIERNFRCRQGEAD
                     IIGYDEHCYLFIEVKYRRNNEKGSPAEAVNYRKQLKICKVADFYRISRKISAAAQIRF
                     DVIAIQNDTIIWHKNAFYYIGSL"
     gene            402..1262
                     /gene="pgeF"
                     /locus_tag="PLU43_01440"
     CDS             402..1262
                     /gene="pgeF"
                     /locus_tag="PLU43_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOO27100.1"
                     /translation="METIRFIRKTGNSKEVLKVNKKNGVTFLTYPLLSQCGGFRHGFS
                     TRLGGVSKGIFESMNLSFSRGDQAEAVMENFKRIADAIGFSHEAMVLSDQTHTTNVRV
                     VTKKDAGSGIMRPKDFFETDGLITNEPGIVLTTFYADCVPLFFIDPVHKAIGLSHSGW
                     RGTVGRMGEKTLQTMKREFGTKPEEVFAAIGPSICADCYEIGEDVASVFAKEFKENAE
                     QILFPKGNGKYRLDLWKANEQILEQAGILPDRLAVTDICTCCNPKLLFSHRASNGQRG
                     NLAAFLEICE"
     gene            complement(1270..2457)
                     /locus_tag="PLU43_01445"
     CDS             complement(1270..2457)
                     /locus_tag="PLU43_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO27101.1"
                     /translation="MPNIEQLEDAMQVAPLKLIVHPSCTELGQQVDEYLVNYRRQINQ
                     PHKSSPAYYDYERTTYLTDFVCPRFGSGEGKSVIRETVRGRDVFILADVCNHSLTYTV
                     NGLENHMSPDDIFQDIKRIIAAIAGKAHRVTVVMPFLYESRQHKRYGRESLDCAQALV
                     ELKNMGVYNIVTFDAHDPKVQNSIPLCGFDSFVPPYQFIRALIHYEKDLIIDKDNLVV
                     ISPDEGALPRAVYFAGVLGVDTGMFYKRRDYSTIVNGKNPIVAHEFLGDEIDGKDVII
                     IDDMISSGGSMLDTAKQLKTMNAKRVFICCTFGLFTEGLDDFDKAYEKCYFDKIITTN
                     LTYRPIELEDRPYYIEANMSKFLASIIDFLNHDVALSKVHTPTEKIKSILEKYNSREE
                     EEY"
     gene            2687..4195
                     /locus_tag="PLU43_01450"
     CDS             2687..4195
                     /locus_tag="PLU43_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO27102.1"
                     /translation="MRGLDTQVRKYRRQIFREIADLAYNSTNIIDDIEALPYKIVKAD
                     EPEFWENIYRERAVAREHCRLAMGMSLRPENQPVHVSQGVEESNIAEKYYEPPLMQVI
                     PSACNACPENRYVVSNECMGCVARPCKEVCPKDAIYFENGKSHIDYEKCIRCGKCKAV
                     CPYDAISHKVRPCMDACGVNAIESDEYGRAKINPEKCVSCGMCMVSCPFGAIADKSQI
                     FQLIRAMQSGKKIIAEIAPAFVGQFGTDVSPQKVKSALKELGFYEVYEVAIGADAGAM
                     AEAKHYVEEVSTGRLPFLLTSCCPSWSMMAKNLFPETIESISNELTPMVATARYIKET
                     HADAHVVFIGPCAAKKLEASRRAVRSDVDFVITFEELMGMFEAKEITFDTLAEEAVED
                     ATGAGRGYGIAGGVASAIEACIHAYYPGTEVKVEHAEGLADCRKMLMLAKAGRKSGCL
                     IEGMACPGGCVAGAGTNIPITQAAKEVLGFKGQAQKAIPYENTKEESNDESH"
     gene            4182..>4682
                     /locus_tag="PLU43_01455"
     CDS             4182..>4682
                     /locus_tag="PLU43_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO27103.1"
                     /translation="MSHIRTRFAPSPTGRMHVGNLRTALYAYLIAKHEGGSFLLRIED
                     TDQERYVDGAVDIIYRTLEKTGLVHDEGPDKDGGVGPYVQSERQASGIYLKYAKQLIE
                     EGKAYYCFCSKERLESLTRTVGGKEISVYDKHCLHLTKEEIEENLASGKPYVIRQNNP
                     NEGTTTF"
BASE COUNT         1425 a          863 c         1187 g         1207 t
ORIGIN      
        1 ataagcatat gacaaatgga aagaaaataa ataatcgaag gatcggaacc gatcatgaaa
       61 cgctggcggc tgcctatctg gaaaaacagg gtgtaacaat tattgaacga aattttcgct
      121 gcaggcaggg cgaagcggat attatcggat atgacgagca ctgctatctg tttattgaag
      181 tgaaataccg gagaaataat gaaaagggca gtcctgcaga ggcagtgaat tacagaaaac
      241 agctgaagat ctgtaaagtg gctgattttt acaggataag ccgcaaaata agtgctgccg
      301 cacagatcag atttgatgta atagcaatcc agaatgatac gatcatatgg cataaaaatg
      361 cattttatta tattggaagc ctgtaatgag gagagttatg aatggaaacg atacggttta
      421 tcagaaaaac aggaaacagc aaagaagtgc tgaaggtaaa taaaaaaaat ggagtcactt
      481 ttctgacata tccgctgctt tcacagtgcg gcgggtttcg tcatggtttt tccacacgac
      541 ttggcggtgt cagtaaaggg atctttgaaa gcatgaatct aagcttttcc agaggagatc
      601 aggcagaggc tgtaatggag aatttcaaaa gaattgcaga tgcgatcgga ttttctcatg
      661 aagcaatggt tctgtcggat cagacacata cgacaaatgt cagagttgtg acaaaaaagg
      721 atgcgggcag cggcattatg cggccaaaag atttttttga aacggatggg cttataacca
      781 atgagccggg gatcgtcctt acgacgtttt atgcggactg tgtacctctg ttttttattg
      841 atcctgttca taaggcaatc ggcttgtctc attcaggatg gaggggaacg gtcggcagaa
      901 tgggtgaaaa aacactgcag acgatgaagc gggaattcgg aacaaaaccg gaagaggtat
      961 ttgccgcgat cggtccgtcc atctgtgcgg actgctatga gatcggtgaa gatgtggcat
     1021 ctgtttttgc aaaagaattc aaggaaaatg cggagcagat tttgtttccg aaaggaaacg
     1081 gaaaatacag gctggatcta tggaaagcaa atgaacagat actggaacag gcagggatcc
     1141 tgccggaccg gcttgccgta accgatatct gtacatgctg taatcccaaa cttttgtttt
     1201 cacatagggc aagtaacgga cagcggggga atctggctgc ttttctggaa atatgtgaat
     1261 aaagggagat taatattctt cttcctcacg ggaattatat ttttcaagta tggatttgat
     1321 cttttcggtt ggtgtatgca ctttggaaag ggcaacatca tgattgagaa aatcaataat
     1381 ggatgccaga aatttgctca tatttgcttc aatataataa ggacggtcct caagttctat
     1441 cggacgataa gtcagatttg tcgtaataat tttatcaaaa taacattttt cataagcttt
     1501 atcaaaatcg tcgagtcctt ccgtaaaaag tccgaaggta cagcagataa atacccgttt
     1561 tgcattcatt gtctttagct gctttgcagt atcgagcata ctgccgccgg atgagatcat
     1621 atcatcaata atgatgacat cctttccgtc aatttcatca ccgagaaatt catgggcaac
     1681 gatcggattt ttaccgttca caatagtgga ataatcacgt cttttataaa acattcccgt
     1741 atcgacacca agaacacctg caaaataaac agctctcggg agagcgcctt cgtcaggact
     1801 gataacgacg agattgtcct tatcaataat cagatctttt tcataatgga tcagtgcgcg
     1861 gatgaactga tagggcggta caaaactgtc aaagccgcac agtggaatgg aattctgtac
     1921 tttcgggtca tgtgcgtcga atgtaacgat attatagacc cccatatttt tcagctccac
     1981 aagagcctgg gcgcagtcaa gcgattcccg gccataacgc ttatgctgcc tgctttcata
     2041 aagaaacggc ataacgaccg taacacgatg tgcttttccg gcaattgcag cgatgatacg
     2101 cttgatatcc tgaaaaatat catccggaga catatggttt tcaagaccgt taactgtata
     2161 agtcaggctg tggttacata catcggccag aataaaaaca tccctgccgc gaacggtctc
     2221 cctgataact gacttccctt cgcccgaacc aaaacgggga catacaaaat ccgtcagata
     2281 agtggttctt tcataatcat aatatgccgg tgatgattta tggggctgat tgatctgcct
     2341 tctgtaattg acaaggtact catctacctg ctgaccaagc tcagtacagc ttggatgtac
     2401 aattagcttt agcggagcca cctgcattgc atcttctaat tgttcgatat ttggcatatg
     2461 tatttttctc ctgaaattat attgttttgt ccattacatt tatatcattc cagttaattc
     2521 acgtcaaggg ttaatggatg tgtaatggta ttttattttg aacaaagagc cgtcattcca
     2581 tgaatatcgt gtaatgcctt gattttacag aaaatatatg atacaaatga tagagacaat
     2641 ggcagggtga tgtaagcgtc gacaggaggg gactattatg aataatatgc gcggactgga
     2701 tacgcaggtc agaaaataca gaagacagat ctttcgggaa attgccgatc ttgcatacaa
     2761 ttcgacaaat atcattgatg atattgaagc acttccatat aaaatcgtaa aagcggatga
     2821 accggagttt tgggaaaata tatacaggga acgcgcagta gcaagagaac actgccgttt
     2881 ggcaatgggc atgtccctaa gacctgaaaa tcagccggtg catgtatcac agggtgtgga
     2941 agagagcaat attgctgaaa aatattatga accgcctctg atgcaggtga ttccgtcggc
     3001 ctgcaatgcc tgtcctgaaa acagatatgt tgtcagcaac gaatgtatgg gctgtgtggc
     3061 aagaccatgc aaagaagtct gtccaaagga tgctatctat tttgaaaacg gaaaatctca
     3121 tattgattat gaaaaatgta ttcgatgcgg aaaatgcaag gcagtctgtc cctatgatgc
     3181 tatttcccat aaagtaaggc cgtgtatgga tgcctgcgga gtcaatgcaa ttgagagcga
     3241 tgagtatgga agagcaaaga tcaatccgga aaaatgtgta tcatgcggga tgtgtatggt
     3301 cagttgtccg tttggagcaa ttgcggataa atcgcagatt tttcagctga tccgcgccat
     3361 gcagtcgggg aaaaagatca ttgcggaaat tgcaccggca tttgttgggc agtttggtac
     3421 ggatgtcagt ccgcaaaaag taaaaagcgc actgaaggag cttggttttt atgaggtata
     3481 cgaagtcgcg atcggggcag atgccggggc aatggctgaa gcaaagcatt atgtggagga
     3541 agtatctaca ggccgcctgc cgtttttgct gacttcctgc tgtccttcct ggtcgatgat
     3601 ggctaaaaat ctgtttccgg aaacgatcga gagtatttcg aatgaattga caccgatggt
     3661 ggcaacggca agatatatca aggaaaccca tgcggatgca catgtggtgt ttatcggacc
     3721 atgcgcggca aaaaaacttg aagcgagcag acgggccgtt cgttccgatg ttgattttgt
     3781 tattacattt gaagaactga tggggatgtt tgaggcaaaa gaaattacgt ttgatacgct
     3841 cgcggaagaa gcggttgagg atgcgacggg agcgggccgg ggatatggta tcgcgggcgg
     3901 tgttgcatcg gcgatcgaag cgtgtattca tgcgtattat cccggaacgg aagtgaaggt
     3961 tgaacatgcg gagggccttg cagactgcag aaaaatgctg atgcttgcaa aagccggaag
     4021 aaagagcggg tgtctgatag aaggcatggc atgtcccggc ggctgtgttg cgggagccgg
     4081 aacgaatata ccgatcacgc aggcggcaaa agaggtgctt ggttttaaag gacaggcaca
     4141 aaaagcaata ccatatgaga atacgaaaga ggagagtaat gatgagtcac attagaacaa
     4201 gatttgcgcc gagtccgaca ggcagaatgc atgttggaaa tttaagaacc gcattatatg
     4261 cgtatttgat tgcaaagcat gaaggaggaa gttttctgct gcgtattgaa gataccgatc
     4321 aggaacgtta tgtggacggc gctgttgata ttatataccg gacacttgaa aaaacaggtc
     4381 tggttcatga tgagggacct gataaggacg gcggggtcgg tccgtatgta caaagtgaga
     4441 gacaggcttc cggtatttat ttaaaatatg caaaacagct gattgaagaa ggaaaggctt
     4501 attattgttt ttgcagcaaa gaacgtctgg aaagcctgac acgcactgtt ggcggtaagg
     4561 agatcagcgt atacgacaaa cactgcctgc atcttacaaa agaagaaatc gaagagaatc
     4621 ttgcatctgg aaaaccgtat gtgatcagac agaacaatcc gaacgagggg acgactactt
     4681 tt
//
LOCUS       DAOSYE010000047         4639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12576_length_4639_cov_3.021815, whole genome shotgun sequence.
ACCESSION   DAOSYE010000047 DAOSYE010000000
VERSION     DAOSYE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12576_length_4639_cov_3.021815"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLU43_01460"
     CDS             <1..655
                     /locus_tag="PLU43_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOO27104.1"
                     /translation="IRLFRSKDVEGPYVDEAGQTMTMVDAQNPEYNKEYGLKMMGNYD
                     FPSLKQAYMSPGHNSAFIDDDGKMYVVYHQRFDDGSEFHQPRVHQLFTTKDGWLVAAP
                     FETSGETLKTDGYKNKEVAGTYYVVNHELDISKTIHESEEVKLYSDGTIRKSSDGELI
                     EIGGYTMEKGTPYITIQLDGADYEGVFIEMTDEAGNEVVCFTAAGENNKTIWGVHYK"
     gene            674..2179
                     /locus_tag="PLU43_01465"
     CDS             674..2179
                     /locus_tag="PLU43_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO27105.1"
                     /translation="MVKLYVNEKEKKGHINPELYGHFSEHLGRCIYEGLYVGEDSEIP
                     NKNGMRTDVVEALRAIEIPVLRWPGGCFADEYHWKDGIGPKEKRKKMVNTHWGGVVED
                     NSFGTHEFFELCDQLGCKTYVNGNVGSGTVQEMSEWVEYITFDGMSPMADLRKENGRE
                     KPWKIDYFGVGNENWGCGGNMTPQYYANEYRRYQTYVRNYHPENLIKKICGGANVADY
                     YWTKEVLKTAFDNVPECAHGLMDGLSLHYYVHPEGWQIKGSATQFDDAAWYKTLSKAL
                     YMEELINKHGTIMDEYDPEKTIGMIVDEWGCWFTVEEGTNPGFLYQQNTIRDALAAGL
                     TLNIINKHCDRVKMANLAQMVNVLQSVILTEGPKMILTPTYHVFHMYRYHQDADLVAS
                     HLEETEPIGTEEAYMVPQLQESVSVGADGIVNITVNNLSVTDEKELFISFMELKPASV
                     EAFIVTGNMTAHNTFEEPDTVKEEKFEAFEITEQGIKAALPPCSVVLFRVK"
     gene            2181..2435
                     /locus_tag="PLU43_01470"
     CDS             2181..2435
                     /locus_tag="PLU43_01470"
                     /inference="COORDINATES: protein motif:HMM:NF039366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HOO27106.1"
                     /translation="MQGTGCRKCLFDQIDQNSYIKSIQNRIDSLDDEIRTVSKVYEKR
                     LAVCGTCRYLKDDFCGACGCFVKLKAAIAVNRCPYEKWKE"
     gene            complement(2500..3420)
                     /locus_tag="PLU43_01475"
     CDS             complement(2500..3420)
                     /locus_tag="PLU43_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOO27107.1"
                     /translation="MLHIKSLDEGLPIFKALGSDIRIAIIKILLENKGMNMNELASRL
                     NITNGALTSHIKKLEEAGIITISSESSGHGNQKKCSVYVDKLLVDFDSDEDSKNVYQT
                     SIKIGHYSDFNVYPTCGLASSTAIIGEVDDTRYFAHPDRYNADILWFTKGYVEYVIPN
                     FIPSSQKIDQITISTELSSEAPGSNDVWPSDISFFINDVCIGMWTSPGDFGDVKGIFT
                     PDWWYLNWNQYGLLKLLVINKKGTFVDGLQISNVSINDFSLDYRSTIKFKMAVKEDSA
                     HVGGLTIFGKTFGNYNQDINIRINYSPLSE"
     gene            3888..>4639
                     /locus_tag="PLU43_01480"
     CDS             3888..>4639
                     /locus_tag="PLU43_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27108.1"
                     /translation="MKKKLLSVLLSTAMVASLLAGCGSAASTEAASDSDTEAAADDTA
                     DEASTDDATEEDSEEVEPDAVTTVGDANGTHMEMWSFVELHNTYYGKMVEKWNEENPD
                     RTIEVTFTTYPYSDMHNKLLMSLQTGEGAPDLCDVEVGQFPNVVAGVDQWLYPLDDAA
                     SPYMDTMVQARMDTYAGADGNHYGAPFHVGATVMYYNLAALEEAGFTQADVDAIETWD
                     DYTALGEKYIAAVGEDGKYFTSVDTGGVDWMWL"
BASE COUNT         1449 a          790 c         1190 g         1210 t
ORIGIN      
        1 gatccggctg ttccggtcaa aagatgtgga agggccgtat gtagacgaag ccggacagac
       61 aatgacaatg gtagatgcac agaatccgga atataataaa gaatatggac tgaagatgat
      121 gggcaattat gactttccga gtttgaagca ggcatatatg tcgccggggc ataattcagc
      181 attcattgat gacgacggaa aaatgtatgt tgtatatcac cagcgttttg acgatgggtc
      241 ggaatttcat cagccgcggg tgcatcagct ttttacaacg aaagacggct ggctcgttgc
      301 ggcgccgttt gagacatcgg gagagacgct gaaaacagac ggctacaaaa ataaggaagt
      361 ggcaggcact tattatgttg ttaatcatga attggatatc agcaaaacga tccatgaatc
      421 ggaagaagta aagctctatt cagatgggac gatcaggaag agcagtgacg gggaattgat
      481 agaaatcggc gggtatacga tggaaaaggg aaccccgtac attaccattc agttagatgg
      541 cgccgactat gaaggtgttt ttattgaaat gacggatgaa gcgggcaacg aagtcgtttg
      601 ttttacggct gcaggggaaa acaataaaac gatatggggc gttcattata aataaaaaag
      661 gagaagagag tatatggtga aattgtatgt aaatgaaaaa gaaaaaaagg gacatatcaa
      721 tcctgaactt tacggacatt tttccgaaca tcttgggaga tgtatttatg agggtctgta
      781 tgttggggag gattcggaaa taccgaataa aaacggaatg agaacagatg tcgtggaagc
      841 gctcagggcg atcgaaattc cggtactcag atggccggga ggctgctttg cggatgaata
      901 ccactggaag gacggcattg gtccgaaaga aaaaagaaaa aaaatggtaa atacgcactg
      961 gggcggagtt gtggaggata acagctttgg cacacatgag ttttttgaac tgtgtgatca
     1021 gctgggatgt aaaacgtatg tcaacggcaa tgtcggaagc ggtacggtac aggagatgtc
     1081 ggaatgggta gaatatataa catttgacgg gatgtctccg atggcagact taagaaaaga
     1141 gaacggcagg gaaaaaccgt ggaaaataga ttattttggc gttggaaatg aaaactgggg
     1201 atgcggagga aacatgaccc cgcagtatta tgccaatgaa tatcgccgtt accagacgta
     1261 tgtcagaaat tatcatccgg aaaacctgat caaaaagatc tgcggcggtg ccaatgtggc
     1321 tgattattac tggacaaagg aagtgctgaa gactgctttc gataatgtgc cggaatgtgc
     1381 gcatggactg atggacggac tttctcttca ctattatgtt catccggagg gctggcagat
     1441 caaaggcagt gcaacgcagt ttgacgatgc ggcatggtac aagacgcttt ccaaagcgct
     1501 ttatatggag gagctgatca ataagcacgg aacgatcatg gatgaatatg atccggagaa
     1561 aacgatcggt atgatcgtag acgaatgggg atgctggttt acagtggaag aaggaacgaa
     1621 tccaggtttt ctttatcagc agaatacgat cagggatgcg cttgcagccg ggcttacatt
     1681 aaatattatt aataagcatt gtgatcgtgt aaagatggca aatcttgcgc agatggtcaa
     1741 tgtgctgcag tcggtcatcc tgacggaagg gccgaaaatg atattgacgc cgacctacca
     1801 tgtatttcat atgtaccggt atcatcagga tgcggatctt gttgcaagtc atctggaaga
     1861 aaccgaaccg atcggaacag aagaggccta tatggttccg cagctgcagg aatcggtatc
     1921 tgtcggagcg gatgggatcg taaatattac ggtcaacaat ctgtcagtta cagacgaaaa
     1981 agaactgttt atcagcttta tggaattaaa gcctgcgagt gtggaagcgt ttatcgtaac
     2041 gggaaatatg acggcacata atacgtttga agaaccggat acggtaaagg aagagaagtt
     2101 tgaagcgttt gaaataacgg agcagggaat caaggcagct ctgccgccat gcagcgtcgt
     2161 attattcagg gtaaaataaa atgcagggaa caggatgcag aaaatgtctg tttgatcaga
     2221 tcgatcaaaa cagctacata aaaagtattc agaataggat agacagcctg gatgacgaga
     2281 tccggaccgt ttccaaagtg tatgaaaaga ggctggcggt atgcggaacg tgccgttatc
     2341 taaaagacga tttttgcgga gcctgcggct gctttgtaaa gcttaaggcg gcaattgcgg
     2401 tcaacagatg cccgtatgaa aaatggaaag aatagaaaag aaaacagaac ggcagcaggg
     2461 ccttgtgact ctgctgccgt tttgttttct acaatccgat tattcggaaa gaggactgta
     2521 attgatacgg atgttgatat cctgattgta attgccgaat gtttttccga agatcgtcag
     2581 tccgccgaca tgtgccgaat cttcttttac tgccattttg aatttgatcg tactgcggta
     2641 atccagactg aagtcattga tcgaaacatt ggagatctgc agaccgtcta cgaacgttcc
     2701 ttttttgttt atgacgagga gcttcagcag accgtattga ttccagttta aataccacca
     2761 gtccggcgtg aagattcctt ttacatcacc gaaatcgccg ggacttgtcc acatgccgat
     2821 acagacgtcg ttgatgaaaa agctgatatc ggaaggccat acgtcattag agccaggggc
     2881 ttcggagctt aactccgtgg agatcgtgat ctgatcgatc ttttgtgagc tgggaatgaa
     2941 attagggatg acatattcta catatccttt tgtaaaccag agaatatccg cattgtatct
     3001 gtccggatgg gcaaaatacc ttgtatcatc cacctctccg ataatcgctg tcgatgaggc
     3061 caggccgcat gtcggatata cattgaagtc ggaataatgt ccgattttga tagaagtctg
     3121 gtatacgttt tttgaatctt cgtcggaatc aaagtctacg agcagcttat ctacgtaaac
     3181 ggaacacttc ttttgattcc cgtgaccgga ggattcgctg gagatcgtaa tgatgccggc
     3241 ttcctccaat tttttgatgt ggcttgtcaa tgcgccgttt gtaatattta aacggctggc
     3301 aagctcgttc atgttcatcc ctttgttttc cagcagtatt ttgataattg cgattctgat
     3361 atcagaaccc aatgccttaa agatcggaag gccttcatcc agtgatttaa tgtgaagcat
     3421 aaaatattct actcctttga taaaaaattc tattgattgt tgataataaa aaataggata
     3481 ataaaagcag aagtaatata taggcataaa agatgtataa ataaagcttc tggataaaag
     3541 agaaaaaaga tgtttaatat caacattttt agtataacgg gataaaaaaa aatagtcaat
     3601 cataaatagt ttagtttttt ctaaaatttt tataatatat aaaagttaag attaatctaa
     3661 aataatatat attttaataa aatatatgtt gacaagggaa tatacatatt gtacaattct
     3721 tatataagaa cgtaatattt ggaagcgaaa tatacaaaat gcatatattt gttgaatatt
     3781 tataaacaat taattatgta aaaaaattgt ttgttttcaa aacacgttca aaagtaaact
     3841 attttatatt agagcaaatc attgcaaagc aaaaggagga tttattaatg aaaaagaagt
     3901 tacttagtgt tcttttaagt acagctatgg tagcatctct gctggcaggg tgtggttctg
     3961 ccgcatcaac ggaagcagca tccgattcgg atacggaagc cgcggcagac gacacggctg
     4021 acgaagcttc gacggacgat gcgacagagg aagacagtga ggaagtggaa ccggatgcgg
     4081 taacaactgt tggtgatgcg aatggtacac atatggaaat gtggtctttt gtggaactgc
     4141 ataatacgta ttacggaaaa atggttgaaa aatggaatga agaaaatccg gacagaacga
     4201 tcgaagttac ctttactacg tatccttatt cggatatgca caataaactg ctgatgtctt
     4261 tgcagacggg agagggagcg cctgatcttt gcgacgtgga agtgggtcag ttcccgaatg
     4321 tggttgccgg tgttgatcag tggttatatc cgcttgatga tgcagcgtca ccatatatgg
     4381 acacaatggt acaggctcgt atggatactt atgcgggtgc ggacggaaac cattacggtg
     4441 ccccgttcca tgtaggtgca acggttatgt attataatct ggcagcactt gaagaagcgg
     4501 gctttacgca ggctgatgta gatgcaatag agacatggga tgactatacg gcactcggag
     4561 aaaaatatat tgctgcagtc ggagaagatg gaaagtactt cacttctgtt gatacgggcg
     4621 gagttgactg gatgtggct
//
LOCUS       DAOSYE010000048         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12705_length_4604_cov_3.310178, whole genome shotgun sequence.
ACCESSION   DAOSYE010000048 DAOSYE010000000
VERSION     DAOSYE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12705_length_4604_cov_3.310178"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1158
                     /locus_tag="PLU43_01485"
     CDS             133..1158
                     /locus_tag="PLU43_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27109.1"
                     /translation="MKLKHKAVLGLMVLAAAVLLCGCKMSPFYNPVAEESDIDDDEEN
                     LIVVGISQLGSESVWRSANTLSIQSAISKENGYFPIYNNARQKQEKQIKAIRGFISQR
                     VDYIVFAPLVEDGWDTILQEAKDADIPVILMDRKISVEDESLYVTRVGTDARKEGEKA
                     GYWLEEELARTDRGTDDINIVVLQGTRGSSVQIDRTAGFYFVADKHENWHILEQVDAD
                     FTTSKGKEVMEQMLKKYQDIDVLMSQNDDMTFGAIEAINDAGLTTGINGDIMVISTDA
                     VYDALLKVKDGIINADIECNPEQGELVSEVIQKLERGETVKKEYIVDEKVFTIDNVDQ
                     YLDTRTY"
     gene            1173..2801
                     /locus_tag="PLU43_01490"
     CDS             1173..2801
                     /locus_tag="PLU43_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27110.1"
                     /translation="MLKVFLVEDESNMRKGLRDNIPWQQCGYEFVGEASDGEMALPLI
                     RKVIPDVLITDIKMPFMNGIALSQVVTREFPDMKIIIISGYDEFEFARQAIQIGVEQY
                     LLKPITRSTMQKVLLEVREKIENEREQKSYMEKFQDEHHEYEQFSRRYFFEQIFEGKL
                     SVQQIYEEAQKLSLEMDASCYNIVLVSMQEKRANMENRGPDSEICERQQEEMLRFFLR
                     FSEYMVFRWNINTYGILIKSDVVQMKEYTDRCILGIKRICEEAEPYLEWYVAEGTPVE
                     RLSLLSECYRNVNHIFSYRFLVPLRHILSQNIISELLPGNEEGNYESLELAKIDPQII
                     RSFLSKGGKDEVEGFVDSYFLSLQDALKSRLFRDYLILNIRFITLTYIESLGFSQTEF
                     LQEANVDKEQEHSAGLAEIKGYILNIIDSALQFRDRQNNNQGKYILKKALDYIERNYA
                     QETLSLNEVASVINVSANYFSAVFSNEMKITFVEYVTQKRMEKAKRLLRQTDKHSGEI
                     ATEVGYKDAHYFSFVFKKTQGYTPREYRNGKKDF"
     gene            complement(2803..3888)
                     /locus_tag="PLU43_01495"
     CDS             complement(2803..3888)
                     /locus_tag="PLU43_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO27111.1"
                     /translation="MTPKYIRLSNILKEQISSNIRKGIYVLPTEEKLCKKYGVSRQTV
                     RHALEILIDEDLIERRQGSGTYIKDTSPNSAKGQVAVLLTHDNEYIYPSMLGDILFAL
                     NRENYSSTLYLTENKTAKEREFLKHLLNHPVHGIIAEPVRSALPNPNLDLYDLLRERG
                     TVILFLSGLYPNFPAFPYVKSDDFYGGYILGKYLLQRRHHVITGIFRQDDHKGIQRYL
                     GLITALTDASAAFKDENIGWFSSDELTRLQEKQDTGFLAEFIRSRLNTCTAVVCHNDE
                     IAYWLMKELKTAGISVPADVSIVSFDNSYLSNFGAQRITSLSHKTHELGRTAAAGLVS
                     LLQGKPFTSVSLSWHLVTRSSDKIHEN"
     gene            4380..>4604
                     /locus_tag="PLU43_01500"
     CDS             4380..>4604
                     /locus_tag="PLU43_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO27112.1"
                     /translation="MKKKLLSVLLSTAMVAVLLAGCGSSASTEPAATEEAATEEVAEE
                     PAAEEEAAPADDTAAADDTAATESTGELIKV"
BASE COUNT         1414 a          800 c         1170 g         1220 t
ORIGIN      
        1 atgaattgcg gagcggagta tggaatgacg attgattctg aaaagggaaa gggcacgctg
       61 atcaaaatta cgattccggc ggagcattcc gacagtacaa aggggtaagg taaagtgtgg
      121 gtaatgagtc aaatgaaatt aaaacataaa gctgttttag gtttgatggt actggctgcc
      181 gcagtcctgc tgtgcggctg caaaatgtcg ccgttctaca atcctgttgc ggaagagagc
      241 gatatagatg atgatgaaga aaatttgatc gtagtgggta tttcccagct cggcagtgaa
      301 tccgtatggc gttcggccaa tacactttcc attcagagtg cgatttcaaa agaaaacggc
      361 tattttccaa tatacaataa tgcaagacag aaacaggaaa agcagatcaa ggcgatccgg
      421 ggttttattt cacagagagt ggattatatt gtgtttgcgc cgcttgttga agatggctgg
      481 gatacgattt tacaggaggc gaaggatgcg gatattcctg ttattctgat ggaccgcaag
      541 atttccgtag aggacgaatc gttatatgtg acaagggtcg gaaccgatgc aagaaaagaa
      601 ggagagaagg ccggatactg gctggaggag gagcttgcaa ggacggaccg cggaaccgac
      661 gatattaata tcgtagtgct gcagggaaca aggggttcgt ctgtacagat cgaccgtacg
      721 gccggatttt attttgtggc agacaaacat gaaaattggc atattttaga acaggtcgat
      781 gcagatttta caacgtcgaa gggcaaagag gtcatggagc agatgctgaa aaaatatcag
      841 gacattgatg tactgatgtc acagaatgat gatatgacct ttggagcgat agaggcgatc
      901 aatgatgccg gtctgacaac aggaatcaac ggagatatca tggttatttc cacggatgcg
      961 gtatacgatg cactgctgaa agtaaaggac ggcattatca atgcggatat tgaatgcaat
     1021 ccggagcagg gagagctggt ctctgaagtt attcagaagc tggagcgcgg agagacggta
     1081 aaaaaggagt atattgttga tgaaaaggta tttacgatcg acaatgtgga tcagtatctg
     1141 gatacaagaa cttattagga tgggagagtg ttatgttaaa ggtctttttg gtagaagatg
     1201 agagcaatat gagaaagggc ttgagagata atattccctg gcagcagtgc ggctacgaat
     1261 ttgtcggcga ggcaagcgac ggggaaatgg cgctgccgtt aatcagaaaa gtaataccgg
     1321 atgttttgat tacggatatc aaaatgcctt ttatgaacgg gatcgcgtta agccaggtag
     1381 tgacacgcga atttcctgat atgaagatta ttattatcag cggatatgat gaatttgagt
     1441 ttgcccgcca ggcgattcag atcggcgtgg agcaatatct gttaaagccg atcacgagaa
     1501 gtacaatgca gaaagtactg ctggaagtac gtgaaaaaat cgaaaatgag cgggaacaga
     1561 aaagctacat ggagaaattt caggatgaac atcatgagta tgaacagttt tccagaagat
     1621 atttttttga acagatattt gagggcaagc tgtccgtcca gcagatttat gaggaagcac
     1681 agaagctttc cctggaaatg gatgcatcct gctataatat tgttcttgtc agcatgcagg
     1741 aaaagagggc caatatggag aaccggggtc cggattcgga gatctgcgaa cggcagcagg
     1801 aggagatgct gcgctttttt ctgcgttttt ccgagtatat ggtattccgc tggaatatca
     1861 acacatacgg gattctgatc aagtctgatg ttgtgcagat gaaggaatat acggaccgct
     1921 gtattttagg gatcaaacgg atctgtgaag aagcggagcc ttatctggaa tggtatgtgg
     1981 cggagggaac accggtcgag agactgagtc tgctgtcgga atgctatagg aatgtaaatc
     2041 atattttttc ttatcgtttt cttgtacctt tgcggcatat tttatcgcaa aatattattt
     2101 ccgaacttct gccgggcaat gaagagggca attacgaaag tctggagctg gcaaagatag
     2161 atccgcagat tatccgaagc tttctttcaa agggaggcaa agatgaagtg gaggggtttg
     2221 tggacagtta ttttttaagt cttcaggacg cgctgaaatc aagactgttt agggactatc
     2281 ttattttgaa tattcgattt attacattga cctatatcga atcccttggt ttttcacaga
     2341 cagagttttt acaggaagca aatgtggata aggaacagga acattcggcc ggtcttgctg
     2401 aaataaaagg ctatattctg aatataatcg acagtgcgct gcagtttcgc gacaggcaga
     2461 acaataatca ggggaaatat atcctgaaaa aagcactgga ttatattgag cggaattatg
     2521 cacaggaaac gctttccctt aatgaagtgg caagtgtcat aaatgtaagc gcaaattatt
     2581 tcagtgctgt tttcagcaat gaaatgaaaa taacatttgt tgaatatgtc acacagaaac
     2641 gtatggaaaa ggcaaagagg ctgcttagac agacggataa gcactccggg gaaatagcga
     2701 ctgaggtagg ctataaggat gcacactatt tcagttttgt tttcaaaaaa acgcagggtt
     2761 atacgccgcg tgaataccga aacgggaaaa aggatttctg agtcagtttt catggatctt
     2821 atcgctgctt cttgttacaa gatgccagga cagacttacg gatgtgaagg gctttccctg
     2881 cagaaggctg acaaggccgg cggcggcggt tctgcccagt tcgtgtgttt tatgggagag
     2941 agaggtgatc ctctgggctc cgaaattgct taaatagctg ttgtcaaaag aaacaatgct
     3001 gacatctgcc ggtactgaaa taccggcagt ttttaattcc ttcatcagcc agtaagcgat
     3061 ctcatcgtta tgacatacga cggctgtgca ggtatttaac ctgctgcgga taaattctgc
     3121 gagaaagccg gtatcctgct tttcctgcag ccgggtgagc tcatcggagg aaaaccagcc
     3181 gatgttttcg tccttaaaag cagccgaggc gtctgtcaga gcggtgatca gaccgagata
     3241 ccgctggatt cccttatggt catcctgtct gaatataccg gtgatcacgt gatgtcttct
     3301 ttgcagcaga tattttccca aaatgtatcc gccgtaaaaa tcgtcgcttt tcacataggg
     3361 aaatgcagga aagttcgggt acagtccgga caggaacaga ataacggtac cccgttcccg
     3421 aagcagatcg tataggtcca ggtttggatt gggcagagcg gaacggacag gctctgcgat
     3481 gattccgtgt acgggatggt tcagaagatg ctttaaaaat tcccgttctt ttgctgtttt
     3541 gttttcagtc agataaagcg tagaggaata attttccctg tttaacgcaa ataaaatatc
     3601 gccaagcata ctcggataaa tatattcatt atcatgggtc aacagaacag cgacctgtcc
     3661 ttttgcagag tttggagaag tatccttgat ataagtcccg cttccctgtc tgcgttcgat
     3721 caggtcctca tcgatcagga tttccagggc atgacgtacg gtctgtctgc tgacaccgta
     3781 ttttttacac agcttttctt ctgtgggaag cacgtagatg ccttttcgta tattggagga
     3841 gatctgttct tttaatatat tggaaagccg gatgtattta ggcgtcataa aatacgttac
     3901 ctcatctcat taaaattctt aataacaaca tacaatagaa cggagcagtc tgcaagtttt
     3961 cattttttgt atagaaggag attattttcg gatttttaga aaattttctg ccggatacct
     4021 ctataaaaaa atgaaaaaat cctcttctca tatgaaaaaa gtcacctttt cgtttttcca
     4081 ctgatgaaat ttacaactga atattttaca taatatgtaa tgaaaatatg aaaaatagag
     4141 agattttttt aattaaatcg gaaaaaatca tacaaaaaaa agtcaaaaca aatatttgta
     4201 ttataattca tagtttgtta aaataatttg tattgttcaa tcatacaaaa ttagttagtt
     4261 tgtacagaaa tacggaaaaa agtattgact caacggacat tattgggtaa atttgacctg
     4321 tagaaaccaa ttacggtttt taaacaagag tgactcagag aaagggagga aactaaaaaa
     4381 tgaaaaagaa actcttaagt gtacttttaa gcacagctat ggtagcagta ttactggcag
     4441 gatgtggaag ctcagcatct acggagccgg cagcaacaga agaagcagcg acggaagagg
     4501 tagcagaaga gccggcagca gaagaggaag cagcaccagc ggatgacacg gcagcagcag
     4561 atgatacggc agctacagaa tcaacaggag aactgatcaa agtc
//
LOCUS       DAOSYE010000049         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12712_length_4603_cov_2.867414, whole genome shotgun sequence.
ACCESSION   DAOSYE010000049 DAOSYE010000000
VERSION     DAOSYE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12712_length_4603_cov_2.867414"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PLU43_01505"
     CDS             complement(<1..1617)
                     /locus_tag="PLU43_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27113.1"
                     /translation="MRGFNKGNSEKKPKGKRVKLNGKAALQCDPIEWEAFQQKKEQDQ
                     EKKERKKFEWDRPLVDEFWTIDDDLHAPSAEDKAEYVGETMKPVASKQLEPEEARKFE
                     QNIINSTIMEDEFLLMESARREHAAKSCEKAQESIYDKFAKEDQEVEKELAGKLDSVN
                     RTAASKTDVRAIGSSVDHKIIIQSEELKHKRKALNSETSKKKIRRRIPKKAGSELSEK
                     QAGFMSRKKSDNIFGQEKNQPSKFREKSANWKNNDEMKMTDGESMGFAETIKSFFGHF
                     SSFSPLQWATAVMAVIIFFTGITTTAVYANYQSEQNKAVALASLSKFEEEDTETVETT
                     IVDEDTALAAAETEETEEKELSLVLSSVEKDLKIKLVDEDDTLVKGVLWGVTVTDADE
                     NVSENEDDDEDGIIHLTDISAGEYSVQLNENDELSGYILPTGAQTVSVKAKIEYTVIQ
                     DIKEEIKTEKEVDATVEDANGNQEVDVETGAALTDTVEWVESTKTANGDSYVEASVVL
                     ANTAKATQRTGTFVAALNALRCAVSRGIAAD"
     gene            complement(1688..2557)
                     /gene="rfbA"
                     /locus_tag="PLU43_01510"
     CDS             complement(1688..2557)
                     /gene="rfbA"
                     /locus_tag="PLU43_01510"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOO27114.1"
                     /translation="MKGIILAGGSGTRLYPLTKAVSKQMMPVYDKPMIYYPLSILMLA
                     GIQEILIISTPRDLPMFRELFGSGGQLGLRMSYAVQETPRGLADAFLVGEEFIGSDSV
                     ALILGDNIFYGQSFSRLLRVVAAREKGATIFGYYVRDPREYGVVEFDENGIALSIEEK
                     PEHPKSNYAVPGLYFYDNDVVKIAKNVKPSARGEIEITSVNNAYLSRGDLHVETLGRG
                     FAWLDTGSHDMLLDAADFVSAFQKRQGMYISCIEEIAYRRGFISREQLLKLAEPLMKT
                     EYGKYLTDVANGL"
     gene            complement(2557..3627)
                     /gene="rfbB"
                     /locus_tag="PLU43_01515"
     CDS             complement(2557..3627)
                     /gene="rfbB"
                     /locus_tag="PLU43_01515"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOO27115.1"
                     /translation="MRTYLVTGGAGFIGSNYIQYMFQKYGDTIRIINVDALTYAGNLE
                     NLKSVEKSDRYLFVKADICDKEAISKIFVQNDIDRVVHFAAESHVDRSIRNPEVFVQT
                     NVLGTLVLLNCAKEAWETADGFKPDKKFLHVSTDEVYGSLEKEGTFFYETTPYDPHSP
                     YSASKASSDLLVKSYIDTYQFPANITNCSNNYGPYQFPEKLIPLIINNTLHGRPLPVY
                     GDGKNVRDWLYVMDHAKAIDMVTEKGRPGEKYNIGGHNEKQNIEIINTIIDTIYEMLP
                     DSDPRKQHVNKELITYVADRKGHDRRYAIAPDKIKKEIGWVPDTMFEEGIRKTIRWYF
                     ENEDWMKNVTSGEYQKYYKEMY"
     gene            complement(3771..>4603)
                     /locus_tag="PLU43_01520"
     CDS             complement(3771..>4603)
                     /locus_tag="PLU43_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HOO27116.1"
                     /translation="NVDIQSMNLLNYDAIPEDAAGIIVLAPTSDYSEEDAQKVSDYLD
                     NGGKAIFITSWTQEGLPEFTGIFEKYGMTLAEGIVVDPEANYYYQNPYYLLPDVSGST
                     ITSSLVSDKRMVFIPYAQGITVEDTKEDDESDDAPDIKQIFKTSDKAYSKTDVHDMQT
                     WEKEDGDVEGPFALGVYVTQSVGDEEMKFLWITSENFISENVNATVAGSNFELITNMV
                     SQMTEHETSVSIPVKDYEAANLTVPKSVFFAGGAVVMLLIPLITLVAGFMIWFSRKKK
                     "
BASE COUNT         1124 a         1257 c          787 g         1435 t
ORIGIN      
        1 gtccgccgca attcccctgc tgaccgcaca gcgcagcgca ttcagcgcag ctacaaacgt
       61 acccgtcctc tgtgtcgcct ttgccgtatt ggcaagcacc acactagcct ctacatagga
      121 atccccatta gctgtttttg tagattcgac ccactctacc gtatcggtaa gtgcggctcc
      181 tgtttccaca tcaacttcct gattgccgtt tgcatcttcg actgttgcat caacttcctt
      241 ctcagtcttg atctcttctt taatatcctg aattaccgta tattcaatct tcgcttttac
      301 ggaaacagtc tgcgccccgg tcggcagtat ataccctgac agttcatcat tttcattcag
      361 ctgtacggaa tactcccctg cactgatatc tgtcagatga atgattccgt cctcatcatc
      421 atcttcattt tcagacacgt tttcatcggc atccgttacc gtaactcccc acagaacacc
      481 cttaacaagc gtatcatcct catccacaag cttgatcttt aaatcttttt ccacggaaga
      541 caaaacaagc gacagctctt tttcctctgt ctcttcagtc tccgcagctg caagcgccgt
      601 atcttcgtct acgatcgtag tttccacggt ttccgtatcc tcttcctcaa atttactcaa
      661 agaagcaagc gctaccgcct tattctgttc actctgataa tttgcataaa cagctgttgt
      721 tgtaattccg gtaaagaaaa taataacagc catcacagcc gttgcccact gcagcggaga
      781 aaagcttgaa aaatgcccaa aaaatgactt gatggtttct gcaaatccca tactttcgcc
      841 atctgtcatt ttcatctcat cattgttctt ccaatttgct gatttttccc ggaacttact
      901 tggctgattt ttttcctgtc caaaaatgtt gtctgatttt ttgcggctca tgaatccggc
      961 ttgcttttca gacagctcac tgccagcctt tttggggata cggcgtctga tttttttctt
     1021 tgaagtttcg ctatttaaag cttttctttt atgtttcagt tcttccgact gaataatgat
     1081 cttatgatct actgaagatc cgatcgccct tacatctgtt tttgatgcgg ccgtacggtt
     1141 aacagaatca agcttgcccg caagctcttt ttcgacctcc tgatcttcct tggcaaactt
     1201 gtcataaata ctttcctgtg ctttttcaca agattttgcc gcatgttccc ggcgagccga
     1261 ttccattagc aggaattcgt cctccatgat cgtagaattg ataatattct gctcgaattt
     1321 acgggcctct tcaggctcga gctgtttgga agctaccggc ttcatggttt cacccacata
     1381 ttccgcctta tcttccgcac tcggcgcatg taaatcatcg tcaatcgtcc aaaactcatc
     1441 cacaagcggt ctgtcccatt cgaacttctt ccgctccttt ttttcctgat cctgttcttt
     1501 tttctgctga aacgcttccc attcaatggg atcacactgc agcgcagcct ttccgttcag
     1561 ctttactctt tttcccttcg gtttcttttc agaattcccc ttattaaatc ctcgcatcct
     1621 gtcctccctt tcgcaaatcc taagcttgac tattcactat ttatctagca attgtcaatt
     1681 cccccgctta taaaccattt gcaacatccg tcagatactt tccatattct gttttcataa
     1741 gaggctccgc cagcttaagc agctgctccc tgctgataaa tcctcttctg taagcaattt
     1801 cctcaataca ggaaatatac attccctgcc gcttctgaaa cgcactcaca aaatccgccg
     1861 catccaaaag catgtcatga ctgcccgtat ccaaccatgc aaatccccgt ccaagcgttt
     1921 ccacatgcag atctcccctc gaaagatatg cattgtttac acttgtgatc tcgatctcac
     1981 ctctggcaga aggcttcaca tttttagcta tctttactac atcattatca taaaaataca
     2041 atcccggaac cgcataattt gatttcggat gctccggctt ttcctctatg gaaagagcta
     2101 tcccgttctc atcgaattct actacgccgt attctctcgg atccctgaca taatacccaa
     2161 atattgtcgc gcccttttct ctggcggcga caacccgtaa aagccttgaa aaactctgtc
     2221 cgtaaaaaat attgtcacca agtatcaaag ctacactgtc agagccaata aattcttccc
     2281 cgacaaggaa cgcatccgca agtccccgcg gtgtttcctg taccgcatag gacatcctga
     2341 gtccaagctg tccgccgctt ccaaaaagct ccctaaacat cggcagatca cgcggtgtgg
     2401 aaatgatcag tatttcctgt atgccggcca gcatcaatat cgaaagcgga taatagatca
     2461 ttggtttatc atagaccggc atcatctgct tagatactgc ctttgtcaga ggatacagcc
     2521 ttgtaccgga accgccggct aaaataatcc ctttcatcag tacatctcct tgtagtattt
     2581 ctgatattct ccgcttgtta catttttcat ccagtcttca ttttcaaagt accatctgat
     2641 cgttttcctg attccctctt caaacatcgt atcaggcacc cagccgatct cttttttgat
     2701 tttatccgga gcgatcgcgt atcgcctgtc gtggcctttt ctgtccgcca cataagtgat
     2761 cagctcttta ttcacatgct gctttctcgg atcggaatcc ggaagcatct catagatcgt
     2821 atcaatgatc gtattgatga tctcaatatt ctgtttttca ttatgcccgc cgatattata
     2881 cttttcaccg ggtctgccct tttccgtaac catatcgatc gcttttgcat gatccatcac
     2941 atagagccag tcccgcacat ttttaccatc tccatatacc ggcagcggtc tgccgtgcag
     3001 tgtgttgttg ataataagcg gaatcagttt ttccggaaac tgatacggtc cgtaattatt
     3061 actgcagttc gtaatgtttg ccgggaactg atatgtatca atataggatt tcacaagcag
     3121 atctgaagag gccttgcttg cggaataagg actgtgcgga tcatacggag tcgtttcata
     3181 aaaaaatgtt ccctctttct cgagtgaccc gtatacttca tccgtcgata catgcaaaaa
     3241 ttttttatcc ggtttgaaac catctgctgt ttcccatgct tctttcgcac agttaagcaa
     3301 tacaagcgta ccgagcacat ttgtctgtac aaatacttcc gggttgcgga tacttctgtc
     3361 cacatgactt tctgctgcga aatgaacgac tctgtcaata tcgttctgta caaaaatttt
     3421 agagatcgcc tctttatcac aaatatcggc ttttacaaac aaataccggt cacttttttc
     3481 cacacttttc agattttcca gattccccgc ataagtaagt gcatccacgt tgatgatcct
     3541 gattgtatcc ccatatttct gaaacatata ctgtatataa ttcgagccga taaacccggc
     3601 tccgcccgtc actaagtacg ttctcatgct taacctccgc cattgtataa tctcatgcat
     3661 aatctcgtag tattataaca ttaaaaacac aaaaaaacaa cagggcagcc ataattaaac
     3721 aactgccctg ctgttatttg attttcatac tgcatctata actgtgtccg ttattttttc
     3781 tttctgctaa accagatcat aaacccggca accagcgtga tcagcggaat cagaagcatt
     3841 acaaccgcac cgccggcaaa gaacacactc tttggaacag tcaggtttgc ggcctcataa
     3901 tcctttaccg gaatggaaac gctcgtctca tgttccgtca tctggctgac catattcgtg
     3961 atcagttcaa aattactgcc ggcaactgtc gcattgacat tctccgaaat aaagttttcg
     4021 gatgttatcc acaaaaactt catttcttca tcaccgaccg actgcgttac gtagacacca
     4081 agcgcgaacg gaccttccac gtcgccgtct tctttttccc atgtctgcat atcatgaacg
     4141 tctgtcttgg aatatgcctt atcagacgtc ttaaagatct gcttgatatc cggtgcatca
     4201 tccgactcgt cgtcctcttt tgtatcctct acggtaatgc cctgcgcata cgggataaag
     4261 accatccgct tgtcgctgac aagcgaagag gtgatcgtac ttccgctcac atccggaagc
     4321 agataatagg gattttgata ataataattt gcttccgggt ctactacgat accttccgcc
     4381 aatgtcatcc catacttttc aaaaatccct gtaaactccg gcagtccttc ctgtgtccag
     4441 gacgtaataa aaattgcttt tccgccatta tccaggtaat cactgacttt ctgcgcatcc
     4501 tcttccgaat aatccgaagt cggtgccagt acgataatac cggcagcatc ttccggaatc
     4561 gcatcatagt tcagcagatt cattgactgt atatctacat ttt
//
LOCUS       DAOSYE010000050         4582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_12789_length_4582_cov_3.914513, whole genome shotgun sequence.
ACCESSION   DAOSYE010000050 DAOSYE010000000
VERSION     DAOSYE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12789_length_4582_cov_3.914513"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLU43_01525"
     CDS             <1..262
                     /locus_tag="PLU43_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO27117.1"
                     /translation="ELSLVCETKDVPEQTLAREDGWKAFRIQGVLDFSLTGILSKISG
                     LLAGQKIGIFAISTYNTDYVLTKAENFEAALHVLEAEGYSVI"
     gene            complement(271..1149)
                     /locus_tag="PLU43_01530"
     CDS             complement(271..1149)
                     /locus_tag="PLU43_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27118.1"
                     /translation="MLTLNLLKDNSEIVPYNFTHFPVHSTECSLSLYPNLSAASHWHM
                     DLEFITVVKGHMLYAVNGTDYPVKEGECIMVNSRQLHNPHSADNTDCLFHCQLFHPSL
                     LFANENLYHAYVEPVCTDSSMSCILLSEAIDWQSIVISELNRIYEICRQPAAGTELII
                     MSLLYSIWLSLYKNTMPHIGQKNDRRLETLSQMIGYIQKHYPEKIMLHDIARSGGVSR
                     SCCCNLFSELLNQTPNQYLTGYRLEKSTEMLRHTSLSVTEIALQNGFNSSSYYTEVFH
                     RILGCTPSEYLAKLHG"
     gene            1325..2503
                     /locus_tag="PLU43_01535"
     CDS             1325..2503
                     /locus_tag="PLU43_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO27119.1"
                     /translation="MTSVLLLIIYFAFISLGLPDSMLGSAWPSMYGEMGVPVSYAGIV
                     SMIIAGGTIISSLFSDKLIRKIGTGKVTLLSVAMTMVALFGFSFTHSFYGLCLWGLPY
                     GLGGGSVDAALNNYVAKHYKSRHMSWLHCFWGIGATAGPYLMGLCLTNGLRWNSGYRM
                     IGSIQVVLVLALFLSLPLWKLVQKQNQGTNGESRQEDTALTLAQTLQLPGAKSVLTAF
                     FCYCALEITTGLWASSYLVLYKEISAEDAARWAALFYLGITAGRFVCGFITEYFGDKR
                     MVRFGQLIVVIGIVLVLLPVKDPVTLTGLVLIGIGCAPIYPSLLHETPVNFGPENSQS
                     VMGMQMASAYIGSTLMPFLFGFAAEAIGIRFYPIYLFLLIILMFFMVERMNRIQAKRS
                     "
     gene            complement(2500..3189)
                     /locus_tag="PLU43_01540"
     CDS             complement(2500..3189)
                     /locus_tag="PLU43_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27120.1"
                     /translation="MEPNLSKTTAQKLYEQLIRKITFRKALAVILGNSILGIGAAGLR
                     FSLMGNEPYTASNMAISDGLHMGLGNYQLMINILLLIIQLIFGRSYIGFGSIVNMCFL
                     GYIVQFSGFVMELLLKSAAGFSLFQKLIFMLVSLVILSLGVSMYQTADLGVAPYDYLA
                     LGMTDRFPTPYFINRVITDGICVLIIVIAVLSHFLTWETSHLGIGTVLGAFFLGPLIN
                     LFTKWNKRWIR"
     gene            3332..>4582
                     /locus_tag="PLU43_01545"
     CDS             3332..>4582
                     /locus_tag="PLU43_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27121.1"
                     /translation="MIKTEGSSLFYKKAMKLILPIVIQNLLSATISSTDIIMLNYVGQ
                     TAISAVSLASQYTSVLFVFFFGLGTGVTMLCAQYFGKGDMRAIDAVEGIALRFSIVVS
                     AAFAVSALLIPQYMMKVFTNDPELISVGISYLRIVSFSYLCWSVMEIYMAVLKSIGRV
                     AVCTALNMIAFVFNIFLNAVFIFGLFGAPKLGAAGVALGTTLSRIIQLVCCIVVSVKS
                     QDVKLKLSYMFIHNKLLLHDFIHLSLPALGNDVAWGVAFSMYSVIIGHLGSDAVAANS
                     FVIVARNFGTVLCFGVGSATGIILGNEIGENKLEGAREDAKRLMKLTVLSGAIGGLFI
                     LAATPFILQYATLTQTAMHYLKYMLLINTYYVMGAAVNTTLIAGAFRAGGDSRFGFIC
                     DTITMWCYAVPLGFVSAFVIKLPVL"
BASE COUNT         1139 a          914 c         1225 g         1304 t
ORIGIN      
        1 ggaactctct cttgtatgtg aaacgaaaga tgtaccggag cagacacttg cacgggaaga
       61 cggctggaaa gctttccgga tacagggagt actggatttt tcactgacag gtatcttatc
      121 gaaaatttcc gggctgctgg ccggacagaa aattggtatt tttgcaatat cgacgtacaa
      181 tacggattat gttctgacga aagccgaaaa ttttgaggca gcactgcatg tactggaagc
      241 tgagggctat tctgttatat aaaagggctg tcagccatgc agttttgcaa gatactcaga
      301 aggcgtacag ccgagaatac ggtgaaaaac ttccgtatag tagctggaac tgttaaagcc
      361 gttctgcagg gcgatctccg ttacagagag ggaagtatgg cgcagcattt ccgtactttt
      421 ttccagccgg taaccggtca gatactgatt cggcgtctgg ttcagcagtt ctgagaaaag
      481 attgcagcag cagctccggc tgacaccgcc ggatctggca atatcatgca gcatgatctt
      541 ttcaggatag tgtttttgaa tatatccgat catttgggaa agagtttcca gccgcctgtc
      601 gtttttctgt ccgatatgcg gcattgtatt tttgtaaaga gacagccaga tcgaatataa
      661 gaggctcatg atgatcagtt ctgttccggc cgccggctgc ctgcagatct cgtaaatacg
      721 gtttagttcg gaaataacaa tactctgcca gtcaatggct tctgataaaa ggatgcagga
      781 catggaagag tccgtacaga ccggttcgac ataggcatga tagagatttt cgtttgcaaa
      841 gagcagggaa ggatggaaca gctgacagtg aaaaagacaa tccgtattgt cggcagaatg
      901 aggattgtgc agctgtcttg aatttaccat gatacattca ccctctttta caggataatc
      961 cgtgccgttg acagcataga gcatgtgtcc ttttacgacc gtgataaatt ccagatccat
     1021 atgccagtgg ctcgctgcgg acagattcgg ataaagggaa agactgcatt ctgtggaatg
     1081 cacgggaaaa tgggtaaagt tgtatgggac gatttcggaa ttgtccttta acagattgag
     1141 cgtcagcatt gtacagcttc ctttcatggt tttccgtttt tatatttgta cgattgttat
     1201 aacgattttc ataaaagtga tagttcagtc cgaaaatatg gaatataatt atagccaatt
     1261 cattattaca tatttacgga caaatgtcta tttttataag ggaatgtccg ggaaagagga
     1321 taagatgact tctgttttac tgctcattat ttattttgcc ttcatcagtc ttggacttcc
     1381 tgattcgatg cttggttccg catggccgtc catgtacggg gaaatggggg tgcctgtttc
     1441 ctatgcggga atcgtcagta tgatcattgc gggcgggacg atcatttcca gtctgttcag
     1501 cgataagctg atcaggaaga tcggaaccgg aaaggtgaca ctgctcagcg ttgccatgac
     1561 aatggtcgcc ttgtttgggt tttcttttac acattccttt tacgggttat gtttatgggg
     1621 gctgccctat ggtctgggcg gtggaagtgt ggatgcggct ctgaataatt atgtggcaaa
     1681 gcattataaa tcaaggcata tgagctggct gcactgcttc tgggggatcg gggcgacagc
     1741 gggtccctat ctgatgggcc tgtgtctgac gaatggtctg cgctggaaca gcggataccg
     1801 tatgatcgga agtattcagg tcgtgcttgt gcttgcgctg tttctgtcac tgccgttgtg
     1861 gaagcttgta cagaaacaga atcaggggac gaacggagaa agccgtcagg aagatactgc
     1921 cctgacactt gcacagactt tgcagcttcc gggagctaag tctgttctga ctgcgttctt
     1981 ctgttactgt gcactggaga tcacgacagg gctgtgggca agcagttatc ttgtactgta
     2041 taaagaaatt tccgcggagg atgctgccag atgggcggcg ctgttctatt tggggatcac
     2101 ggccggccgg ttcgtctgcg gatttattac ggaatatttc ggagataagc ggatggtaag
     2161 gtttgggcag ctgatcgttg tgatcgggat cgtactggtg cttctgcctg ttaaagatcc
     2221 ggtaacattg accggactgg ttttgatcgg tattggctgt gcgccgattt atccaagcct
     2281 gctgcatgag acccctgtca attttggtcc ggaaaattcc cagtctgtga tgggaatgca
     2341 gatggccagc gcttatatcg gctcgacact gatgccgttt ttgttcggat ttgctgcgga
     2401 agccattggt atccgttttt atccaatcta tctgttttta ctgatcattt tgatgttttt
     2461 tatggtggag cgtatgaacc gaatacaggc aaaaaggagt taacggatcc atcgcttgtt
     2521 ccatttcgtg aacagattga tgagcggccc gaggaagaat gcaccgagta cggtgccgat
     2581 gccaagatgg ctggtttccc atgtgagaaa atgactcaat acggcgatga cgataatgag
     2641 aacgcagatg ccgtcggtga tgaccctgtt gataaaataa ggcgtcggga aacggtcggt
     2701 catgccaagt gccagataat catagggggc aacgccaaga tctgccgtct ggtacatgga
     2761 gacgccaagt gatagaataa caagtgagac aagcataaat ataagttttt gaaagagtga
     2821 aaagccggct gcggatttta gcagcaattc catgacaaag cctgagaatt ggacgatata
     2881 accgagaaaa cacatattga caatggaacc gaagccgata taggatctgc cgaaaatcag
     2941 ctggatgatc agcagaagga tattgatcat cagctgatag ttgccgagtc ccatatgcag
     3001 tccgtcgctg attgccatat tggaggccgt atagggttcg tttcccatca gggaaaagcg
     3061 aagtccggca gcaccgattc caaggatact attgccaaga atcacagcca gcgcttttct
     3121 gaaggttatt ttccggatca gctgttcata cagcttctga gcagttgttt ttgataaatt
     3181 cggttccata ttgattccct ttcgttttgt tttgaaattg ttccgaaaga gcggctgctc
     3241 ttatggaagt cacttataaa gtataagacc gacagaaaaa aatagcaatc atgaatatga
     3301 gaaaaatacg atttcaggaa aggcagtgta catgataaaa acagaaggca gctctttatt
     3361 ttataagaag gcaatgaagc tgatcctgcc gatcgtcatt cagaatctgc tgagtgccac
     3421 gatcagctcg accgatatca ttatgctgaa ttatgtagga cagacggcga tttcagccgt
     3481 atcgcttgca tcacagtata cgagcgtttt atttgtgttt tttttcggcc ttggcaccgg
     3541 tgttacaatg ctgtgtgcac agtacttcgg caagggagat atgcgggcga tcgatgctgt
     3601 tgaggggatc gcgctgcgtt tttctattgt cgtgtcggcg gcctttgccg tatccgcact
     3661 gctgatcccg cagtacatga tgaaggtatt caccaatgat ccggaactga tctctgtcgg
     3721 gatctcttat ctgcggatcg tcagtttcag ctatttatgc tggagcgtga tggaaatcta
     3781 tatggctgtt ttaaaaagta tcggcagggt cgctgtctgc actgccttga atatgattgc
     3841 gtttgtgttc aatatttttc tgaatgccgt gttcattttt ggtctgttcg gagcaccgaa
     3901 gcttggtgcg gcaggcgtgg cgcttgggac gacactttcg aggatcattc agctggtctg
     3961 ctgtattgta gtgtctgtga aaagccagga tgtgaagctg aagctgtctt atatgtttat
     4021 ccataataag ctgctgctgc atgacttcat tcatctgtct ctcccggcac ttggaaatga
     4081 tgttgcatgg ggagttgctt tttcaatgta ctcggtcatt atcgggcatc ttggctccga
     4141 tgccgtagca gcgaattctt ttgttatagt ggcgcgaaat ttcggaacgg ttctctgctt
     4201 tggtgtcggc agtgcgaccg ggatcatttt aggaaatgag atcggggaga ataagcttga
     4261 aggagcaaga gaggatgcaa aacgcctgat gaagctgacc gtattaagcg gtgcgatcgg
     4321 aggacttttt attcttgccg caacaccgtt tatcctgcaa tatgcaacat tgacgcagac
     4381 ggccatgcat tatctgaaat atatgctctt gatcaatacc tattatgtaa tgggagcagc
     4441 ggtcaataca acgctgattg caggagcgtt ccgtgcgggc ggtgacagcc ggttcggttt
     4501 tatctgcgat acgatcacaa tgtggtgcta tgcggtgccg cttggatttg tatctgcgtt
     4561 tgtgatcaag cttccggtgt ta
//
LOCUS       DAOSYE010000051         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13134_length_4495_cov_3.195270, whole genome shotgun sequence.
ACCESSION   DAOSYE010000051 DAOSYE010000000
VERSION     DAOSYE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13134_length_4495_cov_3.195270"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLU43_01550"
     CDS             complement(<1..541)
                     /locus_tag="PLU43_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOO27122.1"
                     /translation="MYQINFEKPICIHFIGIGGISMSGLAEILLEEGFSVSGSDSKTS
                     KLTKMLEDKGATLFYGQRASNIQDCVELVVYTAAIKKENPELEAAAARKIPMLTRAEL
                     LGQIMKNYKVPIAVSGTHGKTTTTSMISSILLANESDPTISVGGILKEIGGNIRIGKS
                     DLFITEACEYTNSFLSFFRK"
     gene            1017..2291
                     /locus_tag="PLU43_01555"
     CDS             1017..2291
                     /locus_tag="PLU43_01555"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOO27123.1"
                     /translation="MIKKEMIAMLLAGGQGSRLGILTSKVAKPAVSFGGKYRIIDFPL
                     SNCINSGVDIVGVLTQYQPLRLNTHIGIGIPWDLDRNIGGVTVLPPYEKSGNSEWYTG
                     TSNAIYQNIDYMEMFNPDYVLILSGDHIYKMDYEVMLDFHKANNADVTIAAMPVPYEE
                     AKRFGIVITDENRKITEFEEKPENPRSNLASMGIYIFNWKTLKESLIVKSEQPALDFG
                     KHIIPYCHKNGARIFAYEYNGYWKDVGTLNSYWEANMELIDIIPEFNLYEEYWKIYTK
                     SEALPPQYVSRDSVVEKSIIGAGTNVYGKIYNSVIGCGVTIGSRSVIRDSIIMNEAVI
                     GEDCELNKAIVAENAVIGNYVKLGVGEEAENETDPNIYAHGITTVGENTVIPDGIIIG
                     KNSIVSGRTEAADYENGMLVGGKTLIKAGDKR"
     gene            2288..3406
                     /gene="glgD"
                     /locus_tag="PLU43_01560"
     CDS             2288..3406
                     /gene="glgD"
                     /locus_tag="PLU43_01560"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOO27124.1"
                     /translation="MRAIGLILAGGNSYRMRELSQKRAVAAMPVAGSYRSIDFALSNM
                     SNSHISRVGVLTQYNARSLNEHLSSSKWWDFGRKQGGLFVFTPTVTIENNFWYRGTAD
                     AIFQNLSFLKNSHEPYVVIVSGDGIYKMDYNKVLEYHIEKKADITIVCKDIAENEEAE
                     RYGIVKLNKDSKIEEFEEKPMIAKSNTISCGIYVIRRRQLIELIEKCAEEDRFDFVRD
                     VLIRYKNLKKIYAYKLNDYWSNIASVEDYFRTNMDFLKPEVRDYFFRQYPDVYSKVDD
                     LPPAKYNVGSNVMNSLISSGSIINGTVEDSVIFKKVFVGNNCEIKNSIILNDVYIGDN
                     THIENCIVESRDTIRANSYYKGDGEIKIVLEKNERYVF"
     gene            3462..3734
                     /gene="spoVG"
                     /locus_tag="PLU43_01565"
     CDS             3462..3734
                     /gene="spoVG"
                     /locus_tag="PLU43_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOO27125.1"
                     /translation="MNITDVRVRKVAKDGKMKAVVSITLDDEFVIHDIKVIEGEKGLF
                     IAMPSRKTAEGEYRDIAHPIRSEAREKIQKVILEGYEKALMEGAAE"
     gene            3883..4461
                     /gene="pth"
                     /locus_tag="PLU43_01570"
     CDS             3883..4461
                     /gene="pth"
                     /locus_tag="PLU43_01570"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOO27126.1"
                     /translation="MYIIVGLGNPKTEYKNTRHNVGFDVIDVLADKYNIVTEIRKHRA
                     FCGKGFVGNQKAVLAMPQTYMNLSGDSVREIVNYYKIDPERELIVVYDDIDLDVGQIR
                     IRKKGSAGGHNGMKSIIGQLGTENFIRIRMGVGAKPKGYDLADYVLGHFSKAERELMD
                     ESIIRATEAIRVIIEDGVDMAMNQFNGSREKE"
BASE COUNT         1403 a          726 c         1134 g         1232 t
ORIGIN      
        1 attttcggaa aaaacttaaa aagctgtttg tatattcaca ggcttccgta atgaacagat
       61 cggatttccc gatccgtatg ttgccgccga tttctttcag tattcctccg acggaaatcg
      121 tcggatccga ttcatttgca agcagaatgg aagagatcat ggaagtcgtc gttgtctttc
      181 cgtgtgtgcc ggaaacggca ataggcactt tatagttttt catgatctgc ccaagcagct
      241 cggcgcgtgt cagcatagga atttttctgg ctgcggctgc ttccagttcc ggattttctt
      301 ttttaatggc ggccgtatag acgaccagtt ccacacagtc ctgaatgttt gaggcccgct
      361 gtccgtaaaa gagagttgcg cctttgtctt ccagcatttt tgtgagtttt gaggttttgg
      421 aatccgaacc cgaaacagaa aagccttctt caagaagaat ttcggcaaga ccgctcatac
      481 tgattccgcc gataccgata aaatggatgc agatcggttt ttcaaaattg atttgataca
      541 tagtttgcac ttcctgacag tttttatttc ggtttttgca taatggaaaa gatgataata
      601 tgcaaaagac aactatgttc atcatagact aaaagaaaca aaaaaacaag aacaatgtag
      661 cgtatccgtc tgaaccaata aaagataagc gaatgtaaat atgtatattt tgtgcaaaaa
      721 atatcaaaag atcaacataa atttgttcac ttttatcaaa aaatatgtta tactataaca
      781 tattaacata aatcagggca aaggaatcga tttccggtat tcaagccggc atgaaatgac
      841 gaaagttgct ttgtttgccg aatgcagcaa gcgggaaatg aaagttttga tatctgcgtg
      901 gagtagtggg ggccaaacat gcagatgaga ggtattttgg ggatacaata cggtatggga
      961 aggttatgac gcagcaaaat ggagcgactc aacaattatg agcaagaggt gattatgtga
     1021 ttaagaaaga aatgattgcg atgttgctgg caggcggcca gggaagccga ctgggcattt
     1081 taacatccaa agtagcaaaa cctgcagtat cattcggagg aaaatacaga atcattgatt
     1141 ttcccctgag taattgtatc aattcaggcg tggatatagt cggcgtattg actcagtatc
     1201 agcctcttcg cctgaacacc catatcggaa tcggtattcc atgggatctt gacaggaata
     1261 tcggaggagt aacggttctt ccgccttatg aaaaaagcgg aaacagcgaa tggtataccg
     1321 gtacgtcgaa cgccatttac cagaatattg attatatgga aatgttcaat cctgattatg
     1381 tattgatcct ttccggtgat catatttaca aaatggatta tgaagtcatg ttggattttc
     1441 ataaagccaa caatgccgat gttacgattg ctgccatgcc ggttccatat gaagaagcaa
     1501 agcggtttgg tattgtgatt accgacgaga acagaaagat tactgaattt gaggaaaaac
     1561 cggaaaatcc aagaagtaat ctggcatcta tgggaattta catatttaac tggaaaactt
     1621 taaaggaatc gctgatcgtg aaatcagagc agcccgcgct tgatttcgga aaacatatta
     1681 ttccttactg tcacaaaaac ggtgcaagga tatttgccta tgagtataac ggctactgga
     1741 aagatgttgg tacgctgaat tcgtattggg aagccaatat ggagctgatc gatattattc
     1801 cggaatttaa tttgtatgaa gaatactgga agatatatac aaaaagcgaa gccctgcccc
     1861 ctcagtatgt gtcgcgtgac agtgttgtgg aaaagagtat tatcggagcc ggtacaaatg
     1921 tgtacgggaa gatttataat tctgtaatag gctgcggggt tacgattggc agccgttcgg
     1981 taatacgcga ttccatcatt atgaacgaag ccgttatcgg ggaagactgc gagctgaata
     2041 aagcgatcgt ggccgaaaat gcggtgattg gaaattatgt aaaattaggt gtcggagagg
     2101 aagcagagaa cgaaactgac cccaatattt atgcgcacgg gattacaact gtcggtgaaa
     2161 atacggtaat tccggacggc attattatag ggaagaacag tatcgtgtcg ggcaggacag
     2221 aagcggctga ttatgagaac ggcatgctgg tcggcggaaa aacattaatt aaggcgggtg
     2281 ataaacggtg agagcgatag gacttatttt ggcaggtgga aacagttaca ggatgaggga
     2341 actttcacaa aaaagggccg ttgcggcaat gcccgttgca ggaagctaca gaagtattga
     2401 ctttgcgctg agtaatatgt cgaattccca tatttccagg gttggtgtgc ttactcaata
     2461 taatgcaagg agtctgaatg aacatctgag ttcttccaag tggtgggatt ttggccgcaa
     2521 gcagggaggt ctgtttgtgt ttaccccgac tgtaacaata gagaataatt tttggtacag
     2581 aggcacggca gatgctattt ttcagaatct ttcctttctg aaaaacagcc acgaaccata
     2641 tgttgtgatc gtttccggcg acggcattta taaaatggat tacaacaaag tactggagta
     2701 ccatattgaa aagaaagcag acattacgat cgtctgtaag gatatcgcag aaaatgaaga
     2761 ggctgagcgc tatggtattg tgaagctgaa taaggattcc aagatcgaag agtttgaaga
     2821 aaagcccatg atagcaaagt ccaatacgat ttcgtgcggt atttatgtca tacgcagaag
     2881 acagctgatc gaactgattg agaaatgtgc ggaagaagac agatttgatt ttgtaaggga
     2941 tgttttaatt cgttataaaa atttaaagaa aatatacgca tataagttaa atgactattg
     3001 gagtaatatc gcgtccgttg aagattattt cagaactaat atggactttt tgaagccgga
     3061 ggtcagagat tatttcttca gacaatatcc ggatgtatat tccaaagttg atgatctgcc
     3121 gccggcaaaa tataacgtcg gttccaacgt catgaacagt ttgatttcaa gcggttccat
     3181 tattaacgga acggtagagg attccgtgat attcaaaaag gtatttgtag ggaataattg
     3241 tgagatcaaa aattctatta ttttgaatga tgtgtatatc ggagataata cacatattga
     3301 gaactgtatc gtggagagca gagatacgat tcgcgccaat tcttattata aaggcgacgg
     3361 ggaaattaaa attgttttgg aaaagaacga acgttatgta ttttaacctt tgatttcagc
     3421 atgtatgaaa tgaatgacaa tgtgggaaag ggagaaggct tatgaatatt acggatgtga
     3481 gggtgcgaaa ggtcgcaaaa gacgggaaga tgaaagcagt tgtatcgatt actttggatg
     3541 atgaatttgt gattcatgat atcaaggtga tcgagggtga gaaagggtta tttattgcaa
     3601 tgccgagccg taaaacggcg gaaggagagt atcgtgatat tgcccacccg atcagatctg
     3661 aagcaaggga aaaaattcaa aaagtaattt tggaaggata tgaaaaagcg ctgatggaag
     3721 gagcggcaga ataggaaggt gttattatga ggcaggtttt taaacctgcc ttattttttt
     3781 ctgtctttct gtggacttct tatttttgtt ttggtatact ttagtggcgc cgggtttgac
     3841 atatggcaaa aggggcaata ccggcaaagg gagagagtac agatgtatat catcgttggt
     3901 ttggggaatc ccaaaacaga atataaaaat acaaggcata atgttggttt tgatgtcata
     3961 gacgtacttg ccgataaata taatattgtg acggaaatca ggaaacatcg ggcgttttgc
     4021 ggaaaaggct ttgttggaaa tcaaaaggcc gtcctggcca tgccgcagac ctatatgaat
     4081 ttaagcgggg atagtgttcg tgagattgtg aattattata agatcgatcc ggagagggaa
     4141 ttaattgttg tctacgacga tatcgatctg gatgtcggac agatccgtat tcgcaaaaaa
     4201 gggagtgccg gcggtcataa cggaatgaaa agtattatcg ggcagcttgg tacggagaat
     4261 ttcataagga ttcggatggg tgtaggtgca aaaccgaaag gctatgacct ggctgattat
     4321 gtgcttggac atttttcaaa ggctgaacgg gagctgatgg atgaaagcat tatcagggct
     4381 acggaggcga tccgcgttat aattgaggat ggcgttgata tggcgatgaa ccaatttaac
     4441 ggcagcaggg aaaaggagtg acggggtgaa agcattactt gatcctttgc atgag
//
LOCUS       DAOSYE010000052         4485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13172_length_4485_cov_3.657788, whole genome shotgun sequence.
ACCESSION   DAOSYE010000052 DAOSYE010000000
VERSION     DAOSYE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13172_length_4485_cov_3.657788"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLU43_01575"
     CDS             <1..943
                     /locus_tag="PLU43_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO27127.1"
                     /translation="GGSLLPAPTNGTSNVYKTTIVLENVGTACAVFSLAIVLSDETDY
                     TYGTTDMAAAAGGYLYIWLPDGAKTTAVQAETGAYTGLVTTITAGTATGLFSKTADTE
                     LPTIETVSPDGTGAPLNGEIAITFGETMHTTTAGSVSFDGGTTVLSSGNWSNSNRTYT
                     VSYTALTNNTVYVISVSGFADISGNIMEADSTHSFTTVVMCGNGAYLVESDGDSAYSA
                     GYTDDGLLHLTVNSGVSGFTFFGVNISVQSGHSGSEVCLFVHTRNGVQRGIMACKGDF
                     DTGGSPYAAFNVRAGDVIEVYFVDGLTNDAGVNPSVL"
     gene            1005..1871
                     /locus_tag="PLU43_01580"
     CDS             1005..1871
                     /locus_tag="PLU43_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOO27128.1"
                     /translation="MLKYGDKVGIVACSDARTKKEEGTLKELTEILTGLGLSLVFSCY
                     IFEKRAVFSGSAEQRAGALNALYKDHDIKAIFDISGGDIANELLAYIDFDLIREDPKP
                     FFGYSDLTTIVNAIYKKTGNASYLYQVNCLVWENGKTQTANFKNSLFNGSNDLYEIKW
                     EFVRGAKVEGIVAGGNIRCFLKLAGTQYMPDLDNKILFLESYGGGVAQMETCLCQLKQ
                     MGVFDKISGLLLGTFTKMEEKDEHPDMTELVLNITGDFDFPIAKTREVGHQNTSKCLI
                     IGKKYVISSLES"
     gene            1946..2158
                     /locus_tag="PLU43_01585"
     CDS             1946..2158
                     /locus_tag="PLU43_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO27129.1"
                     /translation="MPLSLAKMGVPYTIIRIAGREKEKHRLESMGLTPDTIVTIISRF
                     NRYIILDVKGARIGIGAELAKKIILK"
     gene            2239..3372
                     /locus_tag="PLU43_01590"
     CDS             2239..3372
                     /locus_tag="PLU43_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO27130.1"
                     /translation="MSYAYGYAISTGEHYYLIDELLKEADKRMYEKKKLIKSECTAET
                     SRPTVLADYHTFVSIVEKELLTGQSDSRFAFVCSDINNLRCFNDQYGHEEGDHVLAQF
                     IYELGRQPFCLCSYKLYSDKFAFLADISQYTNEQAIECIRDWNTYISGLLNQSYDGNG
                     NFVVKSGLYFVSDPKEPVETMLNKADCARKSSKTSFSDIVLYSEELGQSIKLRSEIIN
                     SFQNAVRKQEFRIFVQPKILRTDKSICSAEVLVRWQRSDGVLLSPNRFVPVFEQTGDI
                     VEMDFYVYEKVFRYLYEKWLAKGAVIPLSVNVSRIHLFMTDDFMERLKELHARYPIPL
                     SLIVFELTESAYIQEIDSAGRFIDKLREMGYQVSMDDFGNGYF"
     gene            3353..3562
                     /locus_tag="PLU43_01595"
     CDS             3353..3562
                     /locus_tag="PLU43_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO27131.1"
                     /translation="METDISEEEAELLLQLIGLVKNMGTTIVCEGVETAENVALLERS
                     ECDIFQGYYYYKPFPLEELSRHLTV"
     gene            complement(3552..4166)
                     /locus_tag="PLU43_01600"
     CDS             complement(3552..4166)
                     /locus_tag="PLU43_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO27132.1"
                     /translation="MKKQTAGRKLYSVREAYMIMYRAFRTMPFFIKAKKNGILQETFS
                     ERLMLAVTEVNQCAMCSYAHTKMALETGMNLEEINAMLSGDLSDVESEEMPAILFAQH
                     YADSRCKPSEKSWNSVTAQYGSQKSIAILGAIRVIMFGNTYGIPFGSLKARFSKKSKK
                     AVDPRSSLFYELAMLVTLLIFIPCTLIQALISAALHLPLISFKP"
BASE COUNT         1298 a          818 c         1129 g         1240 t
ORIGIN      
        1 aggagggagt ctgttaccgg ctccaacaaa tggaacatcg aatgtatata aaacgacaat
       61 cgttttagag aatgtcggta ctgcatgtgc ggtgttttcg ctggcaatcg ttctgagtga
      121 tgaaacagac tatacttatg ggacaaccga tatggctgca gctgccggcg gatatttata
      181 tatctggctg ccggacggag cgaaaacgac agcagtgcag gcggagacag gcgcctatac
      241 gggtttggtc acaacgataa cggcaggaac ggccacgggc ctgttttcta aaacagcaga
      301 taccgagctg ccgacaattg agactgtttc gccggatgga accggggctc ccctgaatgg
      361 ggagatcgcg atcacgtttg gtgaaacaat gcatacaacg accgccggaa gcgtatcttt
      421 tgacggcgga acgactgtac tttccagtgg aaactggtca aacagcaacc ggacctatac
      481 ggtatcttat accgcactga cgaacaatac ggtgtatgtg atcagtgttt ccggatttgc
      541 ggatatctcc ggtaatatta tggaggcaga cagtacccac agctttacga ctgttgttat
      601 gtgcggaaac ggggcctatc tggtcgaatc agacggagat tcggcatatt ctgccggtta
      661 tacggatgat ggtcttttgc atctgacagt gaacagcggt gtatcgggtt tcactttttt
      721 tggcgtaaat atttcggtgc agtcaggcca tagcggcagt gaagtctgtc tgttcgtaca
      781 tacaagaaat ggtgtgcagc gcggaatcat ggcgtgtaaa ggcgatttcg ataccggcgg
      841 cagcccctat gcggctttta atgtccgggc aggtgatgtg atcgaagtct attttgtaga
      901 tggcctgacg aatgacgccg gcgtcaatcc aagtgtgctt taaggcagat tctgtgttat
      961 actaaatgaa aagcgtatat ttgaagacag gagaaggtgc cggtatgttg aaatatggtg
     1021 ataaagtcgg aattgtagcg tgttccgatg ctcgtacaaa aaaagaagaa ggaacgctta
     1081 aggaactgac cgaaatttta accggattag gtctgtcttt ggtattcagc tgttatattt
     1141 ttgaaaaaag agcagtgttc agcggcagtg cagagcagcg ggccggtgcg ctcaatgcat
     1201 tatataaaga tcatgatata aaggcgatct ttgatatttc aggcggagac atagcaaatg
     1261 agttgcttgc atatattgat tttgatctga tcagggaaga tccaaagcct ttttttggct
     1321 acagtgatct gacgacgatt gtgaatgcga tctataaaaa aactggaaat gcatcctatt
     1381 tatatcaggt aaactgtctt gtgtgggaaa atggaaaaac gcagacggcg aattttaaga
     1441 acagtctgtt caatggaagc aatgatctgt atgagataaa atgggagttt gtacgcggtg
     1501 caaaagtcga aggcatagtg gccggcggta atatcagatg ctttctgaaa ttggcaggaa
     1561 cgcagtacat gcctgatctt gacaataaaa tattgttttt agaaagctat ggcggcggcg
     1621 ttgcacagat ggagacatgc ttatgtcagt taaagcagat gggggtattt gataaaatat
     1681 ccggcctgct gttaggtaca tttacaaaga tggaggaaaa agacgaacac cctgacatga
     1741 cggagctcgt tttaaatatt acaggggatt ttgatttccc gattgcaaaa acgagggagg
     1801 ttggtcatca aaatacatca aaatgtttga taatcggtaa aaaatatgta atctcctcac
     1861 ttgaatcata aactaagatg tgttaaaata agcccattag ttagtcaagt ctaactaatg
     1921 ggcttatttt taaggagagg ttattatgcc gttatcatta gcgaagatgg gcgttccata
     1981 tacgatcata cgaattgcgg ggagagaaaa ggaaaagcac cgtctggaaa gcatgggtct
     2041 tacgcctgat acgatcgtca ctataatatc cagatttaac cgctatatta ttttggatgt
     2101 aaaaggtgcc cgaatcggga tcggcgctga gcttgcaaag aaaattattt taaaatagga
     2161 aagtcttatg aaaaaaagta ttttctcgca cgtatcggca gggcggctga aaagatcaac
     2221 agaggcagct ccgtgcaaat gagttatgca tatggctatg cgatcagcac aggagaacac
     2281 tattatctga tcgatgagct tctgaaagaa gcagataaaa gaatgtatga aaaaaagaag
     2341 ctgatcaagt ctgaatgtac tgcagaaaca agccgtccga cagtgctggc cgattatcat
     2401 acatttgttt cgatcgttga gaaagaactg cttaccggtc agagtgattc gcgttttgcg
     2461 tttgtctgtt ccgatattaa taatctcaga tgttttaatg accagtatgg tcatgaagaa
     2521 ggagatcatg ttcttgcgca gtttatttat gaacttggca ggcagccgtt ctgcctgtgc
     2581 tcctataaat tatattccga taagtttgca tttcttgcgg atatttccca atatacaaac
     2641 gagcaggcaa tcgaatgtat tcgtgactgg aacacctata tttccggtct tctgaatcag
     2701 tcctatgacg gaaacggcaa ttttgttgtg aaaagcggac tctattttgt ttcggatccg
     2761 aaggaaccgg tggagaccat gctgaataaa gctgattgtg caaggaaatc ttcgaagact
     2821 tcctttagtg atattgttct gtattctgaa gaactcgggc agtcaataaa actgcgttcg
     2881 gaaataatca attcttttca gaatgcggtg cgaaaacagg agtttcgaat atttgtccag
     2941 ccgaaaatac tgcgtacgga taaaagtatc tgcagcgcgg aagtacttgt ccgctggcag
     3001 agaagtgatg gagtattgct gtctccgaat aggttcgttc ctgtttttga acagaccggg
     3061 gatattgtgg aaatggattt ttatgtatat gaaaaggtat ttcgttatct ttatgaaaag
     3121 tggctcgcga agggtgctgt gattccgctg tcggtcaacg tgtcaagaat acatcttttt
     3181 atgacggatg attttatgga gcggcttaaa gaacttcatg cccgctatcc gataccgctg
     3241 tcgctcattg tttttgaatt gacggaaagt gcttatattc aggagattga ctctgccgga
     3301 cgatttatcg ataaactgcg cgaaatggga tatcaggtgt cgatggacga ttttggaaac
     3361 ggatatttct gaggaagaag cagagctgct gctccaattg atagggctcg tcaaaaacat
     3421 gggcacgacg atcgtctgtg aaggtgttga aaccgcagaa aatgtcgctt tactggagag
     3481 atccgaatgt gacatttttc aggggtatta ttactataag ccgtttccgc tcgaagaatt
     3541 aagtcggcat cttacggttt aaagctgatc agcggcagat gcagggcggc agagatcaat
     3601 gcttgtatca gcgtgcaggg tataaaaata agtaatgtaa caagcattgc aagttcatag
     3661 aaaagactgc tgcgcggatc aacagctttt ttggattttt tggaaaaacg ggcttttaag
     3721 cttccaaatg ggatcccata tgtattgccg aacataatga cacggatcgc acctaatatg
     3781 gcaatgcttt tttggctgcc gtattgcgct gtgacggaat tccatgattt ttcggaaggc
     3841 ttgcagcggc tgtccgcata gtgctgtgca aagaggatcg ccggcatctc ctctgattcg
     3901 acatcagaca ggtcgccgga aagcatagcg tttatttctt ccagattcat tccggtttca
     3961 agtgccattt tcgtatgggc atatgagcac atggcacatt gattgacttc cgttaccgca
     4021 agcatcaggc gttctgaaaa ggtttcctgc agaatgccgt tttttttcgc ttttataaaa
     4081 aatggcatag tacggaatgc ccgatacatg atcatatatg cttctctgac ggaatacagt
     4141 ttccgtccgg cagtttgttt tttcattata gtgaaagacc tcctttttta tccgggtgtg
     4201 acttcttcta tcatagcatt tgaaaaaaca gaaagcaaca gaaaaggtta tatctgaaag
     4261 tctgggattg acatcgaaat agaaaaatgt tatcgtatat tgcgttagac ataactaaca
     4321 gattaattaa tagactaact gaaaaggagg agctacttgg atatacaaat ttatgaatca
     4381 atgctgcgaa aacatcaccc tggcagtcac aatgctgaag tatcctggga tcatatggcc
     4441 aggcagttca gagaacggca gagaaaaggc ggaatcgatc atgca
//
LOCUS       DAOSYE010000053         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13225_length_4471_cov_3.437726, whole genome shotgun sequence.
ACCESSION   DAOSYE010000053 DAOSYE010000000
VERSION     DAOSYE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13225_length_4471_cov_3.437726"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLU43_01605"
     CDS             complement(<1..715)
                     /locus_tag="PLU43_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOO27133.1"
                     /translation="MEKKILIHISQLVTPTGSSAKHGKEMQQVKKIEDAAVFIVDGII
                     KKVGPTADILSEMETEGIDTIDLNGHAVLPGFVDSHTHFLFGGYRPEEFMMRLQGIPY
                     IEIHKKGGGIQNTVQATRSLSEEEMYRLGRKRLMDMLSMGVTAVEGKSGYGLDRDCEL
                     RQLKVLQALNRDLPVSIAGTYLGAHSIPEEYKGQPDAYLTFMIREMLPLIRQEKLAEF
                     VDIFCEKGVFTFAQSRRFLQ"
     gene            complement(731..2746)
                     /locus_tag="PLU43_01610"
     CDS             complement(731..2746)
                     /locus_tag="PLU43_01610"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOO27134.1"
                     /translation="MKMSSEQMLISMDDELPEMPAFVEGIRRAPFRGFHLTKEQTKTA
                     LKNALRYISPAYHEQLIPEFLDELYTRGRIYGYRFRPAGKIYGKPIDQYKGKCMEGKA
                     FQVMIDNNLDFDVALYQYELVTYGETGSVCQNWMQYCLIKKYLEELTEDTTLVVESGH
                     PLGLFKSRPEAPRVTITNAMMVGMFDNMKDWEIAEEMGVANYGQMTAGGWMYIGPQGI
                     VHGTYNTLLNAGRQELGIPNDGDLRGHLFISSGLGGMSGAQPKAVEIAGGVGIIAEVD
                     YSRIETRLKQGWVSEWSDDLAHIFALAKEYMDKKQPRSIAYYGNIVDLLQYAVDHQIE
                     IDLLSDQTSCHAAYEGGYCPQGLTFVERTKLLAKDQEAYKEQVDRSLKKHYELIKICV
                     ERGTYFFDYGNSFLKAIYDAGVKEISKNGVDDKDGFIFPSYVEDIMGPHLFDYGYGPF
                     RWVCLSGKPEDLRKTDKAAMECIDRERRYQDLDNYNWIRDAEENRLVVGTQARILYQD
                     AAGRVKIALKFNEMVRDGEIGPVMMGRDHHDVSGTDSPFRETANIKDGSNVMADMAVQ
                     CYVGNAARGMSLVAIHNGGGVGIGKAINGGFGLVLDGSERVDAVIKNGLLWDVMGGVA
                     RRNWARNEHSMETAVEYNKMYAGESQITIPYLAEEALVAKAVEAHFK"
     gene            complement(2764..4089)
                     /locus_tag="PLU43_01615"
     CDS             complement(2764..4089)
                     /locus_tag="PLU43_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO27135.1"
                     /translation="MKLIIPELLYTEKKFKKEYAVLVDGTKIKRIGNAKKLKADYKGA
                     EVIMLDRQVLIPGTVNAHNHCFQSLLRGIAADKPFLEWRDRALYHYSPLMTLEDIYNG
                     ALFAFGEMLKRGVTCVGDFFYLHNYGLESDEAVMRAAKDLGIRLALVRTMYDWDGAPK
                     GYVETVGQAAERTGKLAEKYQTDPMINIIPAPHSLHAASEDMILAGHELAEKLGTKYH
                     MHVAEEPFEIEQVKKEHHGLAPMDYLDQLGVVDDTMVIIHGVWLKPEEIELFGSKGGS
                     LVYCPSSNMFLADGITDIPRMMKAHVKIGLGSDGGCSNNRISVFEEMRMVSLLQKAKT
                     CDALCVNYEQAFTMGTENGAEILGFHTGKIEEGYEADLTGIYLDDLSMMPVSENLEQV
                     LPNIVYSMEPGAVKTVIVQGKTVILNGTLLTVEEERIRENVKQTMKRLG"
     gene            complement(4090..>4471)
                     /locus_tag="PLU43_01620"
     CDS             complement(4090..>4471)
                     /locus_tag="PLU43_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO27136.1"
                     /translation="IRTYKKEKLEEILVKIQAAVKEIEESRKVTIKRKELLNQPCQKL
                     DDEIRAAIGEGILQTGEVKKQMISMAGHDAANMGLLTKAGMIFVKSVGGMGHCRQEYS
                     RKEDIVKAADALFYTLIKLDKELD"
BASE COUNT         1034 a         1279 c          804 g         1354 t
ORIGIN      
        1 cctgcaaaaa acgcctggac tgcgcaaagg tgaaaactcc cttttcacaa aagatatcta
       61 caaactcggc cagcttctcc tgcctgatca atggaagcat ttccctgatc ataaaggtaa
      121 gatatgcatc cggctgacct ttatactctt ccggtatgga atgcgcacca agatatgtgc
      181 cggcaatgct gacaggaagg tcccggttca gtgcctgtaa gactttcagc tgtctcagtt
      241 cacagtcccg gtccagacca tagccgcttt tcccttccac tgccgtcaca cccatggaca
      301 gcatatccat gagtcttttt ctgccaagcc ggtacatttc ctcctcgctc agtgatcttg
      361 tagcctgtac cgtattttga ataccgccgc cttttttatg aatttcaata tacggaatac
      421 cctgcagacg catcataaat tcttccggcc gatatccgcc gaacagaaaa tgcgtatgag
      481 aatcgacaaa tccgggcaga accgcatgac cgtttagatc gatcgtatcg atcccttccg
      541 tttccatttc cgataaaata tcagcagtcg gacctacttt ttttattatt ccgtccacta
      601 taaaaactgc cgcatcttca atttttttaa cctgctgcat ttcttttcca tgttttgcac
      661 tgctgccggt cggtgtgacc agctgtgaga tatgtatcag tattttcttt tccataaaac
      721 acccgtttcc ttatttaaaa tgcgcttcta ccgcctttgc taccaatgct tcttccgcca
      781 gatacggaat tgtgatctga ctctctcccg catacatttt attgtattcc accgccgttt
      841 ccatggaatg ctcatttctc gcccagtttc ttctggcaac gccgcccata acatcccata
      901 gaagaccgtt ctttatgact gcatccacac gttcgcttcc atccagcaca agaccgaaac
      961 cgccattgat cgctttcccg ataccaacac cgccgccgtt atggatcgct accagactca
     1021 tcccccttgc tgcatttcca acatagcact gcaccgccat atctgccatg acattgcttc
     1081 cgtctttgat attggctgtt tcccggaacg gggaatccgt acctgataca tcatgatggt
     1141 cacgtcccat catgaccggt ccgatctctc catctcgtac catttcattg aatttcaatg
     1201 cgatcttaac acggccggcc gcatcctggt aaagaattct tgcctgcgta cccactacca
     1261 gacgattttc ttccgcgtcc ctgatccagt tgtaattatc cagatcctga tatcgtcttt
     1321 ccctgtcgat acattccatg gccgctttgt ctgttttcct tagatcctcc ggctttccgc
     1381 ttaagcatac ccagcggaac ggcccgtacc cataatcaaa aagatgcggt cccataatat
     1441 cttccacata agacggaaaa ataaagccat ccttgtcatc caccccattc ttggaaattt
     1501 cttttacacc ggcgtcgtag attgctttta aaaaggaatt cccatagtca aaaaagtagg
     1561 ttccccgctc tacacagatt tttataagct catagtgttt ttttaacgaa cgatcgactt
     1621 gttccttata tgcttcctga tctttggcca gcagctttgt tctttccaca aaggtaagcc
     1681 cctgcggaca atagccgcct tcatacgccg catgacagga ggtctgatcg gaaagcagat
     1741 ctatttctat ctgatgatcg accgcatact gcagcagatc cacgatattt ccataatagg
     1801 cgatcgaacg cggctgtttt ttgtccatat attcttttgc caaagcaaat atgtgcgcca
     1861 gatcatcaga ccattccgat acccagccct gttttaatct cgtctcgatc ctggaataat
     1921 cgacttcggc aatgattccg actccgcctg caatctcaac cgctttcggc tgtgcgccgc
     1981 tcataccgcc aagtccggaa ctgataaaca gatgtccacg aagatctccg tcatttggaa
     2041 ttccaagctc ctgccggcct gcattcagca gcgtattata cgtcccgtgt acgatcccct
     2101 gcggtccgat atacatccag ccgcccgccg tcatctgtcc ataattggca acacccattt
     2161 cttcagcaat ttcccagtct ttcatattgt caaacatccc gaccatcata gcattggtaa
     2221 tcgtaacacg gggagcctcc ggtctcgatt tgaacagtcc tagcggatgt ccgctttcca
     2281 ctaccaatgt cgtatcttcc gtaagctctt ccagatattt tttgatcagg caatactgca
     2341 tccagttctg acagacactt ccggtttctc cgtaagtaac gagctcatac tgatacagtg
     2401 cgacatcaaa gtccaggtta ttatcgatca tgacctgaaa agccttgcct tccatacatt
     2461 ttcctttgta ttgatcaatt ggtttcccgt aaatttttcc tgccggccgg aaacgatatc
     2521 catagatccg tcctcttgtg tataattcat ccagaaactc aggaatcagc tgttcatgat
     2581 acgccgggct gatataccgc agcgcatttt ttaatgcggt ttttgtctgt tcctttgtca
     2641 ggtgaaatcc acgaaaaggt gctcttcgta ttccttccac gaatgccggc atttccggca
     2701 attcatcatc cattgagatc aacatctgtt cactgctcat tttcatttcc tcctgtcgtt
     2761 tgttcagccg agacgtttca tcgtctgctt tacgttttcc ctgattcttt cttcttctac
     2821 cgtaagaagc gttccgttca gaatcacggt tttcccttgt acaattacag tctttacagc
     2881 ccctggttcc atggaataca caatattggg aagtacctgt tccagatttt cactgaccgg
     2941 catcatggac agatcgtcca aatagattcc ggtaagatcc gcctcatatc cttcctctat
     3001 tttccctgta tggaatccta aaatctccgc tccgttttcc gtccccatcg tgaaagcctg
     3061 ttcataattc acacagaggg catcgcaggt ctttgctttc tgcagcagcg ataccatccg
     3121 catctcttca aatacgctga tgcgattgtt actgcatccg ccgtcgcttc ccaatcctat
     3181 ttttacatgt gctttcatca tccgcggaat atccgtaatg ccgtcggcca gaaacatatt
     3241 gctggaaggg cagtaaacca ggcttccgcc tttgctcccg aacagttcta tttcttccgg
     3301 cttcagccat acaccatgaa tgatgaccat cgtatcatcc actacaccca attgatccag
     3361 gtaatccatc ggtgcaagtc catgatgctc ctttttcacc tgttcaattt caaagggttc
     3421 ctccgccaca tgcatatgat actttgtacc aagcttttct gccagctcat gtcctgcaag
     3481 gatcatatcc tcagatgccg catgcagact gtgcggcgca ggaatgatgt ttatcatcgg
     3541 atccgtttga tatttttccg ccagctttcc ggtacgttct gccgcctgac cgaccgtctc
     3601 cacatacccc ttcggcgccc cgtcccaatc atacatggtt ctcacaagag ccagccgtat
     3661 cccaagatcc tttgccgccc gcatgacagc ctcgtcactt tccagtccat agttatgcag
     3721 ataaaagaaa tctcctacac aagtaacgcc gcgcttcagc atttccccga aggcaaataa
     3781 ggccccgtta taaatatcct ccagtgtcat aagcggggaa taatgataca gtgcgcggtc
     3841 tctccattcc agaaacggct tgtctgccgc gattccgcga agcaggctct ggaaacaatg
     3901 attatgcgca tttaccgttc cgggaatcaa aacctggcga tcaagcatga tcacttcagc
     3961 tcctttatag tctgctttaa gcttctttgc atttccgatc cgtttgattt tcgttccgtc
     4021 cactaaaacg gcatattctt ttttaaattt tttttccgta tataaaagct ccggtatgat
     4081 cagtttcatt tagtccagct ccttatctaa ttttattaat gtataaaaca gcgcatctgc
     4141 tgctttcaca atatcttctt tcctggaata ttcctgtctg cagtgtccca ttcctcctac
     4201 gcttttcacg aatatcatac cggcttttgt aagcagcccc atattcgcag cgtcatgacc
     4261 ggccatgctg atcatctgtt tctttacttc tcctgtctgc aaaatccctt ctccgatcgc
     4321 cgcccggatc tcatcatcca gcttctggca aggctgattc agcagttctt ttcgcttaat
     4381 agttactttg cgcgattctt ctatttcctt taccgctgct tgtattttta caagaatttc
     4441 ttccagcttt tcttttttat aggtacgaat a
//
LOCUS       DAOSYE010000054         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13293_length_4456_cov_2.877301, whole genome shotgun sequence.
ACCESSION   DAOSYE010000054 DAOSYE010000000
VERSION     DAOSYE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13293_length_4456_cov_2.877301"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..814)
                     /locus_tag="PLU43_01625"
     CDS             complement(11..814)
                     /locus_tag="PLU43_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27137.1"
                     /translation="MLDVIDLNIEFHDRSIPETVVYDFGLHLDEGEIVGLVGESGSGK
                     TMSALAIAGLLSRKHMKKSGRILFEGVDLLTCPREKLRSYQGNEISMIFQEPMTSLNP
                     VKKIGWQVEESLRIHTQLSKEERYQKAIRMLTEVELPEARQLYHKYPHELSGGMRQRV
                     MIAAALICDPKLLIADEPTTALDVTVQAQIIELLKKMNRIHHTSVLFISHDLSLVRKL
                     CGRVVVMYQGRIAEQGAAEDVFLRPQEAYTKKLIAAIPKMEKLKYTGEV"
     gene            complement(832..1653)
                     /locus_tag="PLU43_01630"
     CDS             complement(832..1653)
                     /locus_tag="PLU43_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27138.1"
                     /translation="MNKRKKVLNHNLILGGIIACAVVLIVLIGCFYTPYDPDEMDAAS
                     KFAGISWKHIMGCDNFGRDIFSRILEGSRTTMFVALGTVLIGTVFGTLIGALTGYYGG
                     VPDEILMRFNDAVLAFPSILLALVFISVMGSGKYNVMAALGIVFIPSYARIARGEFMR
                     CRNLDYVQSARLMGAGDIRIMFVHILPNTAAVMLSSIVIGFNNAVLAEASMSFLGIGV
                     QPPDSSLGRMLSEAQSFLFVSPQYAIFPGMMIVLMILGFALLSDGLKEYSAKNRQ"
     gene            complement(1668..2609)
                     /locus_tag="PLU43_01635"
     CDS             complement(1668..2609)
                     /locus_tag="PLU43_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27139.1"
                     /translation="MKYLLKKSAALLLTVLIVSFLVFLAFQVIPGDPAVNMLGTSATP
                     KKLAALREQMGLNRPLIVRYASWLHDFFFGDMGTSYSYGTSVRSMIVNKLPITITLTL
                     LSFLLIILFSIPFGIFTAKHEGSIFTKILDIFNQVTMAVPAFFIGILITYLFGLVCRL
                     FTPGGYVSYTEDAAGFFAYLIFPAVSIALPKAAMNTRLLKTSVLKEAKLDYVRTAYSR
                     GNSTNDVLYRHVLKNALIPVVTFLGMTLADIVAGSLIIEQVFSIPGIGRMLITSISNR
                     DYPVVQAIIAIVAFIVIIINFLVDLLYQLIDPRMKGG"
     gene            complement(2649..4178)
                     /locus_tag="PLU43_01640"
     CDS             complement(2649..4178)
                     /locus_tag="PLU43_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27140.1"
                     /translation="MKRKMKGILSVCVLLTLTIALGGCAGGKSENSSQIVVGIPQDLE
                     DSLDPHKSVAAGTKEVLFNVFEGLLKPDSDGNIIPAVAESYDVSEDGKTYTFTLRKGV
                     KFHNGNLVTAEDVKYSIEKAADITGGEPLIEAFSLIESVNILSEDVVEINLSEEDVDF
                     PAYLAMTTAAIIPADNEDPDTNPIGTGPYAFVSRSPQENIVVKRFDDYWGEKAHIENV
                     TFKIESNSDAIVMDLRGGSIDMYARLTSTQVSQLGDGFQVMEGTMNLVQALYLNHAVE
                     PFDNQKVRQAMCYAIDVDQILELTSDGKGTQIGSSMFPAFKKYYLEELSNAYPHDTEK
                     AKELLKEAGYPDGFSMTITVPSNYQPHIDTAEVIVEQLKEVGIQATIQLVEWNSWLSD
                     VYMDRQYESTVIGVDASSMTARALLERFDSEADGNFINYANPDYDALFEQAITCTDDG
                     EQTELYKQMERILSEDAANVYIQDMAEFVVLNDKYDGYTFYPLYVQDISKIYLKDADS
                     E"
BASE COUNT         1218 a         1190 c          861 g         1187 t
ORIGIN      
        1 cagacctttc ctatacttcg ccagtatatt tcagcttttc cattttggga atcgccgcga
       61 tcagtttttt tgtatacgcc tcctgcggcc tcaaaaaaac atcttcagcc gccccctgtt
      121 ctgcgatcct tccctgatac attacaacaa cacggccgca gagctttctt acaagactca
      181 gatcgtgcga aataaacagc acggacgtat gatggatccg gttcattttt ttcagcaatt
      241 cgatgatctg ggcctgaact gttacatcca gtgcagtcgt cggttcatcc gcgatcaaaa
      301 gcttcggatc acagatcagg gctgcagcga tcattacacg ctgtctcata ccgccggaca
      361 gttcatgcgg atatttatga taaagctgcc tggcttccgg cagttccacc tcagtcagca
      421 tccggatcgc tttttgatat ctctcttctt tagaaagctg cgtatgaata cgcaggcttt
      481 cctcaacctg ccagccgatc tttttgaccg gattcagcga agtcatcggt tcctgaaaaa
      541 tcatactgat ctcgtttccc tgataagaac gcagcttttc acgcggacat gtcaaaagat
      601 ccaccccttc aaacaagatc ctgccgctct ttttcatatg ctttctggat aacagccctg
      661 cgatcgcaag cgcggacatc gtcttgcctg atccggactc tccgacgagc ccgacgatct
      721 ctccctcatc aagatgcagg ccgaaatcat atacgactgt ttccggaata ctacgatcat
      781 gaaattctat attcagatcg atcacgtcaa gcatttttca accccctccg tctactgcct
      841 gttttttgcc gaatattctt tcagcccgtc actgagcagc gcaaagccaa gaatcatcag
      901 tacgatcatc attcctggaa aaattgcata ctgcggcgat acaaacaaaa atgactgtgc
      961 ttcagacagc attctgccaa gacttgaatc aggcggctgt acaccgattc ccaaaaaact
     1021 catgctcgct tccgcaagca ctgcattatt aaacccgatc acaattgaag aaagcatcac
     1081 tgccgccgta ttggggagga tatgcacaaa catgatacgg atatctcctg ctcccatcag
     1141 ccgtgcactc tgcacataat caagattcct gcagcgcatg aattctcccc tggctatccg
     1201 ggcgtaactt ggtataaata cgatcccaag cgctgccata acgttatact ttccgcttcc
     1261 catcacactg ataaacacaa gcgcaagaag aatactcgga aaagcgagca ccgcgtcgtt
     1321 gaaacgcatc agtatttcat cgggtacacc accgtaataa cctgtcagcg cgccgatcag
     1381 cgttccgaaa acagtcccga tcaaaactgt ccccagcgca acgaacatcg ttgttctgct
     1441 tccttccagg atcctgctga aaatatcccg tccgaaatta tcgcagccca taatatgctt
     1501 ccatgagatc ccggcaaatt tgcttgcggc atccatttca tccggatcat atggggtata
     1561 aaaacacccg atcaaaacga tcaaaacgac cgcacatgct atgatcccgc ctaagattaa
     1621 attatgattc agtacttttt ttctcttatt catgagataa ctccccatca gccgcccttc
     1681 atccgcgggt cgatcagctg atacagcaga tccacaagaa aattaataat aatcacgata
     1741 aatgcgacga tcgcgatgat cgcctgcacg accggataat cccggttgga aatagaggtg
     1801 atcagcatcc ttccgattcc cggaatcgag aacacctgct ctatgataag acttccggcc
     1861 acaatatcgg caagcgtcat acctaaaaaa gtaacgaccg gtatgagcgc atttttcaat
     1921 acatgcctgt aaagaacgtc attggtggaa ttccccctgc tgtacgctgt tctgacataa
     1981 tccagtttcg cttcctttaa aacagaggtt tttagaagcc tcgtattcat tgccgctttc
     2041 ggaagtgcaa tggagaccgc aggaaatatc agatatgcaa aaaaaccggc agcatcttcc
     2101 gtataagaca catagccgcc cggcgtaaac agcctgcata cgagcccaaa cagatatgtg
     2161 atcagaatgc cgataaaaaa tgcagggact gccattgtga cctgattaaa aatgtccagt
     2221 atttttgtaa atatgctgcc ttcatgtttt gcggtgaaaa taccgaacgg aatcgaaaaa
     2281 agaatgatca gcagaaaaga aagcagcgtc agcgtgatcg tgatcggcag cttgttaacg
     2341 atcatggacc gcacggaagt tccataggaa taggaagtcc ccatatcccc aaagaaaaag
     2401 tcatgcagcc agcttgcgta tctaacgatc agcggcctgt tcagccccat ctgttcccgc
     2461 agggcggcaa gctttttggg cgttgcactc gtaccaagca tattgaccgc cggatctccc
     2521 ggtatcacct gaaaagcaag gaagacgaga aatgaaacaa tcaaaaccgt cagcagcagt
     2581 gccgctgatt tcttcagtaa atatttcata tcccgcctcc cttttctgat tgaagcatgc
     2641 gcgccgcgct attcgctgtc cgcgtccttt aaataaattt tcgaaatatc ctgcacatac
     2701 agcggataga atgtgtagcc gtcgtacttg tcattcagaa caacaaattc ggccatatcc
     2761 tgaatataaa catttgccgc atcctcagaa aggatccgct ccatctgctt gtacagttct
     2821 gtctgttccc cgtcatccgt acatgtgatc gcctgctcaa aaagcgcatc atagtccgga
     2881 ttggcgtaat tgataaaatt accgtccgct tccgaatcaa aacgctccaa aagagctctg
     2941 gcagtcatgg aagaagcatc cacaccgatg accgtagact catactgtct gtccatataa
     3001 acatcgctca gccatgaatt ccactccaca agctggatcg tcgcctgaat accgacttcc
     3061 ttcagctgtt cgacaatgac ctcagccgta tcaatatgcg gctgataatt ggacggcact
     3121 gtgatcgtca tggaaaatcc gtccggatag cctgcttcct tcagcaattc ctttgccttt
     3181 tccgtatcat gcggatatgc attgctcagt tcttccagat aatacttttt aaatgccgga
     3241 aacatactgc ttccgatctg tgttcccttc ccgtcagatg taagctccag gatctggtca
     3301 acatcgatcg catagcacat cgcctgccgg accttctgat tgtcaaacgg ctccaccgca
     3361 tggttcagat acagtgcctg aacgaggttc atcgtccctt ccatcacctg aaagccatca
     3421 ccgagctgtg atacctgcgt cgatgtcagc cttgcataca tatcgatcga tccgccgcgc
     3481 agatccataa cgatcgcatc ggaattggat tcgattttga aggtgacatt ctcaatatgc
     3541 gctttttctc cccagtaatc gtcgaaacgt ttcaccacga tattctcctg cggagagcgg
     3601 gaaacaaacg catacggccc agttccgatc gggtttgtat caggatcttc attgtccgcc
     3661 ggaataatgg ccgccgtcgt cattgcaagg tatgccggaa aatcgacatc ctcttcagac
     3721 aggttgattt caacaacatc ctccgataag atatttacgc tctcaattaa agaaaaagct
     3781 tcgatcaacg gctctccccc cgttatatcg gcagcttttt caatactgta ttttacatcc
     3841 tctgccgtaa cgagattccc gttgtgaaat ttgacgccct ttctcaaggt gaaggtatag
     3901 gttttcccat cctccgatac gtcatacgat tctgctacgg ccggaatgat atttccatct
     3961 gaatcaggct taagtaagcc ttcaaacaca ttgaacaata cctctttagt tcctgccgca
     4021 actgatttgt gtgggtcaag actatcttct agatcttgtg gtattccaac aactatctgc
     4081 gaagaatttt ctgacttacc acctgcgcat ccgcccagtg caatagtcag tgtcagtaac
     4141 acacacacag aaagaatgcc tttcatcttt cttttcatca tgtgttatct cctcttcttt
     4201 ctatatgaaa tttttaccgg tccctcaatt gataaatatt tgtcaattgt ccggtaatgt
     4261 caatattgta tacaaaaact gtccaaattg caatatcctg ataaaataat ctgtccatga
     4321 atacaaaaaa atacaataca aacctttgca gtccgtattg tatttcacaa aggacaacgt
     4381 cgattgtcca gctatggtat tttatttatc aagcattttt ttctgagaaa aatattcata
     4441 aagttttatt tcatca
//
LOCUS       DAOSYE010000055         4432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13407_length_4432_cov_3.260224, whole genome shotgun sequence.
ACCESSION   DAOSYE010000055 DAOSYE010000000
VERSION     DAOSYE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13407_length_4432_cov_3.260224"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLU43_01645"
     CDS             <1..485
                     /locus_tag="PLU43_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOO27141.1"
                     /translation="LLEVDEKLRRRSFSKMLRELPRQLLPLPVTKKFQSEDKLFRIQS
                     EIIRNLADTESCIIVGKCADYILRDYQNVFRVYIEAPRDYCVKRVISRMHCEEAKAHA
                     LISKTDRYRAAYYEYYTHGNLWTRPENYDFILNSESFGEQNCIQIIKEGVRLKLKKDL
                     "
     gene            complement(512..1408)
                     /locus_tag="PLU43_01650"
     CDS             complement(512..1408)
                     /locus_tag="PLU43_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27142.1"
                     /translation="MSVYTEAEHAIALADTKFWIEHPPVLTKDRYAYGQYHFRLKDYS
                     EAVHWFLIAAESGCEEAWFDIGVCIYYDLLNDSDIPRQYGQYGSCFSKAFAYYENCAS
                     MPSCSSLEKYRLAILYRYRLGTNTDLKKAFQLFQDVTDMHADLTEAHFHAECNYSYEG
                     SDISAGFDVCKLPAGSSYYELAKYYAEGISPVGQDISHARILLKKAHDFHSEEALFYD
                     FTRFGNNFDNYEYQDDIKELYSFLIGLYIRVSEVHPSVKAYERLIRLYENGYPGDTDE
                     RAVSFFQKAERFRKKLHALQDS"
     gene            complement(1419..2156)
                     /locus_tag="PLU43_01655"
     CDS             complement(1419..2156)
                     /locus_tag="PLU43_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HOO27143.1"
                     /translation="MTLEMELYEIVSTIIKNHPKASYYREPVIGYADAEDPLYDQLDE
                     IIGNPQLHPRDMLTGAKTVMVLFLPYSEIVYNSIGRQHLVSSVWSDAYMETNKLLDHI
                     MNVVSEFLSSRGIRSSCEPPTENFDHVSKTARWGHKSNAVIAGIGSFGLNHLLITKKG
                     CMGRMNSLVMDAHIPPTPRTGHEYCLYYQNGGCKACIKNCPSGAIWTDGTIDKKRCDA
                     YLEGKNIHDWQQGCPQCSLGPCALKGF"
     gene            complement(2194..3402)
                     /locus_tag="PLU43_01660"
     CDS             complement(2194..3402)
                     /locus_tag="PLU43_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27144.1"
                     /translation="MEKFIFDDMSFHEDTRLLYEGQHINGMELSPEAFPLFATSAFNM
                     GDLDNVAETYEKKGYTYIRTRNPNRKALADVITFLEQGEDSLIFSSGMGAITTTYFAL
                     LQPGDHFLCNENIYGETFDAIKILLHNYGVETDFAPFDDLDAVRACLKPNTKMLYTEV
                     ASNPTDRLCDIEELAKIAHGAGALLMVDNTFTTPVCVKPLTLGADIIINSLTKFLNGH
                     SDALAGSITVKDKALFDKIHTIRMLTGTSGCPFASWMIFRGIHTADLRIRKQCENAAL
                     LAQALSEHPHVICVNHPSVKSHPQYELGKKLFTSPYAMTGMLSFEMPDDREKINAFMN
                     KLHIAHYAPTLGGIRTTLSYPLLSSHMHVPEKELKKMHISGGLMRVSTGIENADDLIA
                     DFYNALSVFA"
     gene            3614..>4432
                     /locus_tag="PLU43_01665"
     CDS             3614..>4432
                     /locus_tag="PLU43_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOO27145.1"
                     /translation="MNEIKQSLRSIYYHYQKEKVQDMLKDELPQTGSKIKKIVVAGAG
                     LMGASIAQSFPEYGVKTTIYTNQESDYGRARQIIANSQRTLVENQMLTPEQSQKIQEE
                     IGYTTDPQCFADADWIIEAVPEDFQIKKNLLELISDITPEDTLITTNTSAISINALSR
                     CVSSPARFCGTHWLNPPHIIPLVELIRGEETGNFTIELLSELLTGMGKQPVVLKKDIK
                     GFLSNRLQFALLREATYLVENGIADPEDIDRTLKYGNGLRYTCSGPFKIVDFGGI"
BASE COUNT         1254 a          932 c         1027 g         1219 t
ORIGIN      
        1 atctgctgga agtagatgaa aagcttcgca ggcgcagctt cagcaagatg ctgcgtgaac
       61 tgccaaggca gctgctgccg cttccggtaa caaagaaatt tcagtcggaa gacaaactgt
      121 tccggataca gtctgaaatc attcggaatc ttgccgatac ggagtcgtgt attattgtgg
      181 ggaaatgtgc cgactatata ttgcgggact atcagaatgt attccgtgta tatatcgagg
      241 cgccaaggga ttactgtgta aagcgggtca taagccggat gcactgtgaa gaagcaaaag
      301 cccatgcgct gatcagcaag acagaccgtt acagggcagc ctattatgaa tactatacgc
      361 atggaaattt gtggaccaga ccggaaaact atgattttat tttaaacagt gaaagctttg
      421 gagaacagaa ctgtatacag atcataaaag aaggcgtccg cttaaaactg aaaaaggatt
      481 tgtaaggaac atgcactgca tactctgctt atcaggagtc ctgcagtgca tgtagttttt
      541 tgcggaaccg ttcggctttc tgaaaaaagg agacagcccg ttcgtccgta tcgcccggat
      601 atccgttctc atacagcctg atcagacgtt cataggcttt cacggaaggg tggacctcgg
      661 aaacccttat ataaagaccg atcagaaaac tgtaaagttc tttgatgtca tcctgatatt
      721 catagttatc aaaattattg ccaaagcggg taaaatcata gaacagtgcc tcttcgctgt
      781 gaaaatcatg ggcttttttc agcagtattc ttgcatggga aatatcctgt ccgacaggag
      841 agatcccttc tgcgtaatat tttgccagtt cataatagct gctgccggcg ggaagcttgc
      901 aaacgtcaaa gccggctgaa atatcggaac cttcatacga ataattgcat tccgcatgga
      961 agtgcgcttc tgtcagatcc gcatgcatgt cggtaacatc ctgaaacaat tggaaagctt
     1021 ttttaagatc tgtgttcgta cctaatctgt aacgatacaa aatggccaga cggtattttt
     1081 ccaaagagct gcacgacggc atggacgcgc aattttcata ataagcaaaa gcttttgaga
     1141 aacagctgcc atactgcccg tattgccttg gaatgtcgga atcgttaaga agatcataat
     1201 agatacagac tccgatatcg aaccaggctt cttcgcagcc ggactctgct gcgatcaaaa
     1261 accaatggac cgcttcgctg taatctttta aacgaaaatg atactggccg taggcatatc
     1321 tgtcttttgt aaggaccggc ggatgctcga tccagaattt tgtatcggca agtgcgattg
     1381 catgttcggc ttcggtataa acactcatga gtctcctcct agaatccctt taatgcacat
     1441 ggcccgagag agcattgggg acatccctgc tgccaatcgt gtatattctt tccttccaga
     1501 taagcgtcac agcgcttttt gtcgatcgtt ccgtctgtcc agatggcacc ggaaggacag
     1561 tttttgatac aggccttgca gccgccgttc tggtaataca gacaatattc gtgaccggtc
     1621 cgaggggtag gcggaatatg ggcatccatt acgagactgt tcatgcgtcc catacagcct
     1681 ttttttgtta taagcaggtg attcagtcca aaagaaccga ttccggcaat gacagcattt
     1741 gacttatgcc cccatctggc ggttttgctt acgtgatcaa aattttcggt agggggctcg
     1801 cagctgctgc ggatcccccg ggaactgaga aattctgaaa caacgttcat aatatggtct
     1861 aacagtttgt ttgtttccat ataggcatcc gaccatacgg aagatacgag atgctgcctg
     1921 ccgatggaat tgtatacgat ctccgagtaa ggaagaaaaa gcaccataac ggtttttgca
     1981 cctgtgagca tatcccgtgg atggagctgt ggatttccga taatttcatc cagctgatca
     2041 taaagaggat cttctgcatc cgcgtaacca atgacaggct ccctgtaata agaggccttc
     2101 ggatgatttt taataattgt actgacgatt tcatataatt ccatttccag tgtcataagc
     2161 attcctctgc agtttttctc cgatatcgga aacttatgca aatacgctta acgcgttata
     2221 aaagtccgca atcaggtcgt ctgcattttc tattccggtg gatacgcgca taaggcctcc
     2281 ggaaatatgc atttttttca gttctttttc cggtacgtgc atgtgggaag acagcagcgg
     2341 ataagacagt gttgtgcgga taccgcccaa tgtcggcgca taatgggcaa tatgaagttt
     2401 attcataaat gcatttattt tttcacggtc atccggcatt tcgaaactga gcattcctgt
     2461 cattgcatac ggagaagtaa acagtttttt gccaagttca tactggggat gtgattttac
     2521 ggaagggtga ttgacgcata tgacatgcgg atgttcagaa agtgcctgcg caagcagagc
     2581 ggcattttcg cactgttttc tgatgcgcag atctgcagta tgaattccgc gaaagatcat
     2641 ccaggatgca aacggacagc cggaagtacc ggtcagcatt cggatcgtat ggatcttatc
     2701 aaaaagagcc ttgtccttaa ccgtaatgga gcctgcaagt gcatccgaat gaccattcag
     2761 aaattttgtc aggctgttga tgataatatc ggcgcccagt gtcagcggtt ttacacagac
     2821 aggtgttgta aaggtattgt ctaccatgag aagcgcacct gctccgtggg cgatctttgc
     2881 aagctcttca atatcgcaga ggcgatcagt cggattgctt gcaacttccg tatagagcat
     2941 ttttgtattt ggtttcagac aggcgcgaac tgcgtccaga tcatcaaacg gggcaaaatc
     3001 tgtttcaacg ccgtaattat gcagtaatat ttttatggca tcaaaggtct ctccgtaaat
     3061 attttcattg cagagaaaat gatctccggg ctgcagcagg gcaaaataag tagttgtaat
     3121 ggcgcccata ccggaggaaa agatcaggga atcttctccc tgttctaaga aagtgattac
     3181 atcggccaat gccttgcggt ttggatttct tgtacggata taagtatagc cctttttttc
     3241 ataagtttct gccacattgt ccagatcacc catattaaaa gcactggttg caaacagagg
     3301 aaaggcttcg ggagaaagtt ccattccgtt tatatgctgc ccctcataga gcagccgtgt
     3361 gtcttcatga aaagacatgt catcaaatat aaatttttcc atagtaaatc ggactccttt
     3421 tctattgttt cttcatggca ttattataat ctctataaca aatagagagt caatgcttta
     3481 ttttcattta ttttatttgt atgattttac aggaaaagga agtttcaaca atgattatga
     3541 acagacacgg aaaatatcat tgttttgaca gataaattta tatattgact ctgtatttat
     3601 tatagatttt ataatgaacg aaatcaagca gtcattaaga tcgatttatt atcattatca
     3661 gaaagagaag gtacaggata tgttaaaaga cgaattaccg cagacaggat ccaaaatcaa
     3721 aaaaatcgtt gttgcaggag ccggtctgat gggcgcttcc attgcacagt cttttccgga
     3781 atacggtgtg aaaacgacaa tttatacaaa tcaggaatca gattacggca gagccaggca
     3841 gatcattgcc aacagtcagc gcacactggt ggaaaatcaa atgctgacgc cggaacagtc
     3901 gcaaaaaata caggaggaga ttgggtatac gacggatccg caatgctttg ccgatgcgga
     3961 ctggatcatt gaagcggtcc cggaagactt tcagatcaaa aaaaatctgc tggagctgat
     4021 ttcggatatt acgccggaag atacgctgat cacaacgaat acttctgcaa tttctattaa
     4081 tgcactgagc cgctgtgtaa gcagtcctgc acgtttctgc ggaacgcact ggctgaatcc
     4141 gccgcatatc atcccgcttg tagaactgat caggggagaa gaaaccggga attttacgat
     4201 agaactgctt tctgagcttt tgacaggaat gggaaagcag cctgtcgtac tgaaaaaaga
     4261 tatcaaagga tttctttcca atcgtctgca gtttgcactt ttaagggaag ccacgtatct
     4321 tgtggaaaat gggatcgcgg atccggaaga catcgatcgt acgttaaaat acgggaacgg
     4381 cctgcgttat acatgctccg gccctttcaa aatagttgat ttcggaggga tc
//
LOCUS       DAOSYE010000056         4404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13528_length_4404_cov_2.655783, whole genome shotgun sequence.
ACCESSION   DAOSYE010000056 DAOSYE010000000
VERSION     DAOSYE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13528_length_4404_cov_2.655783"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1623
                     /locus_tag="PLU43_01670"
     CDS             118..1623
                     /locus_tag="PLU43_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO27146.1"
                     /translation="MERVTYQEQGVTLLENFSMTIWAGEIMGLVPVNHYGLGALIELL
                     LRNHPLHYGYVYYHEKQVNHWRYPKYGVNRISVIKNKSCLAGKLTVTDNIFVLRPGFK
                     KHVIRQRLLERQLQPFLKDIGIRIRADEYVENLTAFERLVVEILKAVVAGCYLVILED
                     IGTFVSDSELAKLHEILRHYADQGMSFLYIAAHFEEVRQICDRTALMMNGRITKIFQA
                     SDEIPDTLSFECTEDFERHVREQIEKRPDASKLPTAAFEIRHLCYGNIHDLNLRVTQG
                     ECLVVQDMSGSLFQDFLCLLPKGKLKRTEGVLIDGMPCGRAKRHSVAFIQESPVKSML
                     FPDMSYMENLCFTMDHKLPFVWLNKRIQNGIRKEFEQVLGKEVFDMRVEKLSKTQKYD
                     LIYTRILLQNPKVVFCVAPFKGAEVSLRIHIMKLIETLLEKKIAVVILTVNLADSLAL
                     AERVICIRENGEIQSYNKENFGKLPVTTPWLYLYREKNRFSQTADSDTVLE"
     gene            complement(1586..2443)
                     /locus_tag="PLU43_01675"
     CDS             complement(1586..2443)
                     /locus_tag="PLU43_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27147.1"
                     /translation="MKYLEYREKRQQGTFDFPIAFYRVGPAHPRYMMPYHWHPEFELI
                     RILEGNFRMIIDGTPLTAKKGDILFIRDGILHGGTPDACIYECVVFDLKLLLQDNHIC
                     SKQIRSIINHTISIHCSLAGKDPSLLQTADHIFHALREKKNGYEFITQGALYQLFGII
                     LEEHLYDETALPDAASQHHILDFKNVLHYIEEHYTESISLEELAKQAGLSPKYFCRFF
                     RDMTNRTPIDYLNYYRIECACEQLSTTRTSITEVALNCGFNDTSYFSKLFRRYKGITP
                     RQYLNRQFG"
     gene            2632..>4404
                     /locus_tag="PLU43_01680"
     CDS             2632..>4404
                     /locus_tag="PLU43_01680"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOO27148.1"
                     /translation="MAKNRYIGEYPVIGIRPTIDGRRGALKVRESLEEQTMNMAKSAA
                     KLFTDNIKYSNGEPVKVVIADTTIGRVAEAAACEAKFRKEGVAITLTVTPCWCYGAET
                     MDMNPLTIKGVWGFNGTERPGAVYLASVLATHAQKGLPAFGIYGHDVCEADDTSIPKD
                     VMEKLLRFGRAAVAAASMRDKSYLQIGSVTMGIGGSIIDPDFIEEYLGMRVESVDEVE
                     ILRRMEEHIYNEEEYQKALAWIKENCKEGWDKNPDGIKKSDEEKAADWEFIAKMACII
                     EDLYQGNPDLPEGCEEEAVGHNAICGGFQGQRQWTDHWPNCDFPEAMLNTSFDWNGTR
                     EPFILATENDTLNGLGMLFMKLLTNRAQMFADVRTYWSGEAVLKATGYAIEGKAKEAD
                     GFIHLINSGACCLDACGEVKDKDGNAVIKEWYDVTEEDQKTIMDATQWCAADNGYFRG
                     GGFSSRFVTRAQMPATMIRLNLVKGLGPVLQIAEGYTVQLPDEVTDKLWKRTDYTWPC
                     TWFTPKCDGKEGSPFKTAYDVMNNWGANHGAISYGHIGADLITLCAMLRIPVCMHNVE
                     EDKIFRPAAWNAFGMDKEGQDYRAC"
BASE COUNT         1289 a          858 c         1222 g         1035 t
ORIGIN      
        1 gcatgtattt tcaatgcccg gagttgggac cgatgtggag atcatgcttc ctgcgattac
       61 aagcgacagg gaaatcaaaa agcaagaggc gttataatga aggaagaagc gcttaggatg
      121 gagcgagtga catatcagga gcagggcgtc acattgcttg aaaatttcag catgacgatt
      181 tgggcagggg agatcatggg acttgtgccg gtgaatcatt acggcctcgg cgcactgatc
      241 gagctgcttt taagaaacca tccgctgcac tatggctatg tctattatca tgaaaaacag
      301 gtgaatcact ggaggtatcc gaaatacgga gtcaaccgca tcagtgtgat caagaataaa
      361 agctgtctgg ccggtaagct gactgtgacc gataatattt ttgtcctgcg cccgggtttt
      421 aaaaagcatg tcatacggca gcgtctgctg gaaagacagc tgcagccgtt tttaaaggat
      481 atcgggatcc ggatacgggc ggatgaatac gtagagaatc tgacagcatt tgaaaggctt
      541 gtcgttgaaa tactgaaggc tgtcgtagca ggctgttatc ttgtcatatt agaggatatc
      601 ggaacgtttg tcagcgacag tgagcttgca aagctgcatg agatcttaag gcattatgca
      661 gatcagggta tgtcgtttct ttatattgcc gcgcattttg aagaggtcag gcagatctgt
      721 gacaggacgg ctctgatgat gaacggaagg atcacgaaga tctttcaggc atcggatgaa
      781 ataccggata cgctgtcatt tgaatgcacg gaagatttcg agcgccatgt cagggaacag
      841 attgaaaagc gtccggatgc gtcaaagctt ccgacagcgg catttgagat caggcacctg
      901 tgctatggaa atattcatga tctgaatctg cgtgtgactc agggagagtg tcttgttgtg
      961 caggatatga gcggcagcct gttccaggat tttttatgcc tgcttccaaa aggaaaactt
     1021 aagaggacgg aaggtgtatt gatcgacgga atgccgtgcg gcagggcaaa gagacacagt
     1081 gtggccttta ttcaggaatc acctgtgaaa tcaatgctgt ttccggatat gagctatatg
     1141 gaaaatcttt gttttacaat ggatcataag cttccgtttg tctggctgaa caagcggata
     1201 caaaacggga taagaaaaga atttgaacag gtacttggaa aagaagtatt tgatatgcgt
     1261 gttgagaagc tttcaaagac acagaaatat gatctgatct atacgcggat cctgctgcag
     1321 aatccgaaag tcgtcttctg cgttgcacca tttaaaggag cagaagtttc cctgcgtatt
     1381 catattatga agctgataga aacactgctg gagaaaaaaa tcgcggtagt gatactgacc
     1441 gtaaatctgg ccgattcgct ggcacttgcg gaacgagtga tctgtatccg ggaaaacgga
     1501 gaaatacaat catataataa agaaaatttc ggcaagctgc cggtaacgac cccatggctg
     1561 tatttgtacc gtgagaaaaa caggttcagc caaactgccg attcagatac tgtcttggag
     1621 tgatcccttt ataacgccgg aacagtttgc tgaagtagct tgtatcattg aaaccgcagt
     1681 tgagggcaac ctcggtaatg cttgtacgtg ttgtagacag ctgttcacag gcgcattcga
     1741 tccgatagta attcagatag tcgatcggtg tgcggtttgt catgtcgcgg aaaaagcggc
     1801 agaaatattt cggagacaga ccggcctgct ttgcgagttc ctcaagagag atgctttccg
     1861 tataatgttc ttcaatataa tgcagtacat ttttaaaatc caggatatga tgctgtgagg
     1921 cagcgtcagg aagtgctgtc tcatcataga gatgctcttc aagaatgata ccaaacagct
     1981 gatacagagc gccctgtgtg atgaattcat agccgttctt tttttcgcgg agcgcatgaa
     2041 aaatgtgatc ggctgtttgc agcagagacg gatctttgcc tgccaggctg cagtgaatag
     2101 agatcgtatg attaataatg cttcggatct gttttgaaca gatatggtta tcctgcagca
     2161 gcagtttcag atcaaaaaca acacattcat aaatacacgc atccggtgtt ccgccgtgca
     2221 gaattccatc tctgataaac agaatatccc cttttttagc tgtcagcggc gtgccgtcga
     2281 tgatcatacg aaagttgcct tccagaatgc gtatcagttc aaattccgga tgccagtggt
     2341 aaggcatcat atagcgcgga tgcgcaggac cgacacgata gaatgcaatt gggaaatcaa
     2401 aggtaccctg ctggcgtttt tcccgatatt ccaaatattt cacgataggt acgcctccca
     2461 taacaatgaa gataatgaat aattggaaac aatataaaat gtgaatattg ttatacttaa
     2521 ggtccattat aacacatgaa ttccgtaaaa accagcgtta taataagaat atcagtataa
     2581 tcagctgcta taattcatat gatatgacag taaacaaagg aggattttga aatggcaaag
     2641 aacagatata ttggagaata tccggtaatt gggatcagac cgacgatcga cgggcgccgg
     2701 ggagcattga aagtaaggga gtcactggaa gagcagacaa tgaatatggc aaagtctgca
     2761 gcgaagcttt ttaccgacaa cataaaatat tccaacggag agccggttaa ggtagtgatt
     2821 gcggatacaa cgatcggccg tgtggcggaa gccgcggcct gtgaagcaaa gttcagaaaa
     2881 gaaggcgttg cgatcacgct gacggtcaca ccgtgctggt gctacggggc ggagacgatg
     2941 gacatgaatc cgctgacgat caagggtgta tggggattca acggaacaga gagaccggga
     3001 gccgtgtatc tggcatccgt gctggccaca catgcgcaga aaggactgcc ggcattcggc
     3061 atctatggac atgatgtctg tgaggcagat gatacgtcca taccaaaaga tgtgatggaa
     3121 aagctgctca gattcggacg agcggcagtt gcggctgcca gcatgagaga taaatcatat
     3181 ttacagatcg gttctgttac gatgggaatc ggcggttcca ttatcgatcc ggacttcata
     3241 gaggaatatc ttggaatgag agtggagtct gtggatgaag tggaaatact gagacgaatg
     3301 gaagagcata tttacaatga agaggaatat cagaaggcac ttgcctggat caaggagaac
     3361 tgcaaggaag gctgggataa gaatccggac ggaatcaaaa aatcggatga agagaaagca
     3421 gcggactggg agttcatagc aaagatggca tgtatcatcg aggatctgta tcagggaaat
     3481 ccggatctgc cggaaggctg tgaagaagag gcagtgggac ataatgcgat ctgcggaggc
     3541 ttccaggggc agcggcagtg gacagatcac tggccaaact gtgattttcc ggaagcgatg
     3601 ctgaatacat cctttgactg gaacggaaca agggagccat tcatccttgc cacggagaat
     3661 gacacgctga acggtcttgg catgctcttt atgaagctgc tcacgaaccg tgcccagatg
     3721 tttgcggacg tacgtacata ctggagcggg gaagcagtcc tgaaagcgac aggctatgcg
     3781 atcgaaggaa aggcaaagga agcggacgga ttcatccatc tgatcaattc cggagcctgc
     3841 tgtctggatg catgcggaga agtgaaggat aaggacggaa atgccgttat caaggaatgg
     3901 tatgacgtaa cagaagaaga ccagaagacg atcatggatg cgacgcagtg gtgcgcggcc
     3961 gacaacggat acttccgcgg aggcggattc tcctcaagat tcgtaacacg tgcacagatg
     4021 cctgccacga tgatccgtct gaatcttgta aagggactgg gcccggtgct gcagatcgca
     4081 gaagggtata cggtgcagct gccggatgaa gtaacggaca agctgtggaa gcgtaccgat
     4141 tacacatggc cgtgtacatg gtttacacca aaatgtgacg gaaaggaagg aagtccgttc
     4201 aagacagcat atgatgtgat gaacaactgg ggagcgaatc atggagcgat cagctatggt
     4261 catatcggag cggatttgat cacgctgtgt gcgatgttaa gaataccggt ctgcatgcac
     4321 aacgtagagg aggataagat cttcagaccg gctgcatgga atgcattcgg catggataaa
     4381 gagggacagg attacagggc atgt
//
LOCUS       DAOSYE010000057         4379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13648_length_4379_cov_3.163737, whole genome shotgun sequence.
ACCESSION   DAOSYE010000057 DAOSYE010000000
VERSION     DAOSYE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13648_length_4379_cov_3.163737"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLU43_01685"
     CDS             complement(<1..487)
                     /locus_tag="PLU43_01685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO27149.1"
                     /translation="MIADERTEAYIDSLDKGNTVFLNELEAGAKAEHVPVIKRPAQNL
                     LKLLLAMKKPAYILEVGTATGFSALLMAEYGPADCHITTIEKYEKRIPAAKENFKRAG
                     REKQITLLEGEAADILDRLSKEDPESFDLIFMDAAKGQYLRFFPSVLQLLKKGGLLVS
                     DN"
     gene            complement(502..1008)
                     /locus_tag="PLU43_01690"
     CDS             complement(502..1008)
                     /locus_tag="PLU43_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOO27150.1"
                     /translation="MTGKQLTMSFLGTIIRGALLVIAVLVIYKAGMKAYDFGFRIFTE
                     EPMSEAPGRDIEITVSDGDGTKAVSKMLEEKGLIRDAQLFMIQAKLSEYSGDIQPGNY
                     TLNTSMTAEEMLAVLLTDESADAEEETDETDELQEADPLTEAAPADDTGDATEDSVTA
                     EDTETTEN"
     gene            complement(1065..1403)
                     /locus_tag="PLU43_01695"
     CDS             complement(1065..1403)
                     /locus_tag="PLU43_01695"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOO27151.1"
                     /translation="MRMSGNDMDKTIEKLIEQMQRTEAYQNYNSQKETMKNFPELKEK
                     IDTLRRLNLQLQKIPESDSLYEEVERIEQQYEELCEDTRVHDFMQAELDFCRMFQDIL
                     ARITSSMEFE"
     gene            complement(1415..3088)
                     /locus_tag="PLU43_01700"
     CDS             complement(1415..3088)
                     /locus_tag="PLU43_01700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOO27152.1"
                     /translation="MKQQNTNGSRLKIIPLGGLEQIGMNITAFEYEDSIVVVDCGLSF
                     PEDDMLGIDLVIPDVTYLKENEEKVKGFVITHGHEDHIGALPYILKDLNVPVYATRLT
                     IALIDGKLKERNLQKAVKRKIVKFGQSINLGAFRIEFIKTNHSIADASALAIYSPAGI
                     VVHTGDFKVDYTPVFGDAIDLQRFAELGKKGVLALMCDSTNAERPGFTNSERTVGKTF
                     DSIFADHKSTRIFVATFASNVDRVQQIIDSAYKYGRKVVVEGRSMVNIIQTASELEYI
                     KIPENTLIDIEQLKNYPDEQTVIITTGSQGEAMAALSRIATSIHKKVSIKPGDTVIFS
                     SNPIPGNEKAVSKVINELSMKGADVIFQDAHVSGHACQEEIKLLYTLVKPKYAIPVHG
                     EYRHLKAQAKIVQELGFPKENIFVLSSGDVLEFDGEKAAVTGEVPTGAVFVDGLGVGD
                     VGNIVLRDRQHLAEDGILIAVLTLEKGTNQLLAGPDIVSRGFVYVRESDEIMEEARVV
                     VEEALLKLFDRNVTDWGKMKNTMKDALSGYVWKKMKRRPMILPIIIEVE"
     gene            complement(3092..3358)
                     /locus_tag="PLU43_01705"
     CDS             complement(3092..3358)
                     /locus_tag="PLU43_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOO27153.1"
                     /translation="MEKIQFAVDDGKETAEFYVLDQTRINGRDYLLVAESTEDEAEAL
                     ILRDTAPEDAQESVYEIVSDDTELSAVAKVFEEMIGDIEFKQED"
     gene            complement(3358..3804)
                     /gene="ruvX"
                     /locus_tag="PLU43_01710"
     CDS             complement(3358..3804)
                     /gene="ruvX"
                     /locus_tag="PLU43_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO27154.1"
                     /translation="MRIMGLDFGSKTVGVAVSDPLLITAQGVEIIRRDAEKKIRKTLA
                     RIEELIMQYEVDRIVLGFPKNMNASIGERGEKTIEFQQTLIRRTGLEVVLWDERLTTV
                     AADKAMMEGKVRREDRSKYVDKIAAIFILQGYLDYLAGLPGHGEER"
     gene            complement(3806..4069)
                     /locus_tag="PLU43_01715"
     CDS             complement(3806..4069)
                     /locus_tag="PLU43_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOO27155.1"
                     /translation="MQNIGNTQYFNVQTEPETGVKVVLSTVYEALTEKGYNPVNQIVG
                     YIMSGDPTYITSHNNARSLIMKVERDELVEELLTQYIKNNGWK"
     gene            complement(4139..>4379)
                     /locus_tag="PLU43_01720"
     CDS             complement(4139..>4379)
                     /locus_tag="PLU43_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOO27156.1"
                     /translation="IAEAAKRGRRYREWYAGRPLEVLFEEKQEINGQFYQTGYTKEYV
                     KAAVRREEDLCGKIVRGKAERMLTDEILVFSGELE"
BASE COUNT         1027 a         1245 c          765 g         1342 t
ORIGIN      
        1 cattatcaga aacaagcagt cctccttttt tcaaaagctg caggacagac ggaaaaaatc
       61 tcagatattg tccctttgcc gcatccataa atatgagatc aaagctctcc ggatcctctt
      121 tggacaatct gtccaaaata tccgctgctt ccccttccag taacgtgatc tgcttttccc
      181 tgccggccct tttaaagttt tccttcgcag caggaatccg tttttcatac ttttcgatcg
      241 ttgtaatatg acagtctgcc ggtccgtatt ccgccatcaa aagagcggaa aaaccggtcg
      301 ccgttccgac ttccagaata tacgccggtt ttttcattgc cagcagaagc tttagcaggt
      361 tctgtgcagg acgtttgatg accggcacat gttccgcctt tgctccggct tccagctcat
      421 ttaagaatac tgtgtttccc ttgtcgagcg aatcgatata agcctctgtt ctttcatctg
      481 caatcactgt ttgatcctct gctaattttc cgttgtctcc gtatcttccg ctgttacgct
      541 atcctccgtc gcatcgccgg tatcatcggc cggcgcagcc tcggtaagcg gatccgcctc
      601 ctgcaattca tcggtctcat ccgtctcttc ttcagcatca gcagactcat ccgtcagcag
      661 aaccgcgagc atctcctctg ctgtcatcga agtattcagc gtataattac ccggctgaat
      721 atccccggaa tattccgaca gctttgcctg tatcataaag agctgtgcat ccctgatcag
      781 acccttttct tccagcattt tgctgactgc ctttgttccg tccccatcgg aaaccgtaat
      841 ctcgatgtct cttcccggcg cctcggacat cggctcctcc gtaaaaatac ggaaaccaaa
      901 atcatatgcc ttcattccgg ctttataaat aacaagtacc gcaattacta acagcgcgcc
      961 tctgatgatc gtcccgagaa atgacatcgt caactgtttt cccgtcatat agactcctcc
     1021 ttatgcattc cgcagattac cggtctgcca aaaaacaaac gaacttactc gaattccata
     1081 ctggatgtaa tccgggccag aatatcctga aacatccggc aaaaatccaa ttcggcctgc
     1141 ataaaatcat gtacccttgt atcttcacaa agttcctcgt actgctgctc aattctttcg
     1201 acttcttcat acagactgtc ggattctgga attttctgca attgcaaatt cagcctgcgt
     1261 aacgtatcga ttttttcttt tagttccgga aagttcttca ttgtttcctt ctgactgttg
     1321 taattttgat aagcttccgt gcgctgcatc tgttcaatca atttctcaat tgttttgtcc
     1381 atgtcattcc ctgacattct catacaggtc tctcctattc cacttcaata atgatcggca
     1441 ggatcatcgg ccgccttttc atctttttcc atacataacc gctcagggca tctttcattg
     1501 tgttcttcat cttgccccag tcagtaacat ttctgtcaaa cagcttcagc agcgcttcct
     1561 ccacaacaac gcgtgcttct tccataattt cgtctgattc cctgacataa acgaatcccc
     1621 ttgacacaat atcaggaccg gcaagcagct gatttgttcc tttttccaat gtcagcacgg
     1681 caatcaggat cccatcctct gccagatgct gcctgtcacg aagtacgata tttcctacat
     1741 cgcctacacc aagtccgtca acaaatacgg ctcctgtcgg cacttctccc gttacggccg
     1801 ctttctcgcc gtcaaactcc aatacatctc ctgaagataa aacaaaaata ttttccttcg
     1861 gaaagccaag ttcctgtacg atttttgcct gcgctttcag atgacgatat tctccgtgca
     1921 ccggaattgc atatttcggc ttcaccagcg tatagagcag cttgatttcc tcctggcagg
     1981 catgtccgga aacatgcgca tcctgaaaga tcacatccgc gcccttcatg ctcagctcat
     2041 taatgacctt ggaaactgct ttttcgttgc caggtatcgg attggaactg aaaatgaccg
     2101 tatctcccgg cttgatcgat acttttttat gaatgctcgt tgcgatcctg ctcagtgcgg
     2161 ccattgcctc tccctgactg ccggtcgtaa taatgacagt ctgttcgtcc ggataatttt
     2221 tcagctgttc aatatcgatc agcgtatttt cggggatttt aatatattcg agctccgaag
     2281 cagtctgaat gatattgacc atgcttcgtc cttctactac gacctttctg ccatacttat
     2341 aagcagaatc aatgatctgc tgtactctgt ccacatttga tgcaaacgta gccacaaaaa
     2401 tcctggtact tttgtgatcc gcaaaaatac tgtcaaacgt ctttccaacc gtacgctccg
     2461 aatttgtaaa gcccggacgt tccgcattcg tactgtcaca catcagggca agcacgccct
     2521 ttttgccaag ctccgcaaac ctctgcagat cgatggcatc accaaatacc ggcgtataat
     2581 ccactttgaa atcacccgta tgcacaacga taccggccgg cgaatagatc gccagtgcac
     2641 ttgcatccgc aatactgtga tttgttttaa taaattcgat ccggaaagca cccaaattaa
     2701 tcgactgtcc gaatttgacg atttttcttt ttaccgcttt ctgcagattg cgctccttca
     2761 gctttccgtc gatcagcgcg atcgtcagcc tggtcgcgta gaccggtacg ttcagatcct
     2821 tcaatatata gggcagtgcc ccaatatgat cttcgtggcc atgtgtaata acaaagcctt
     2881 ttactttttc ctcgttttcc ttcagatatg taacgtccgg gatcacaaga tcgattccga
     2941 gcatatcatc ctccggaaac gacaagccgc aatccacgac aacaatactg tcctcatatt
     3001 caaaggcagt aatattcatt ccaatctgtt caagacctcc taacggaatt attttcagcc
     3061 tgcttccgtt cgtgttttgt tgtttcaata gttaatcctc ctgcttaaat tcaatatccc
     3121 ctatcatttc ttcaaatacc tttgccactg ctgaaagctc cgtatcatca gacacgatct
     3181 catatacgct ttcctgcgca tcttccggag ccgtatctct taatatcagt gcttctgcct
     3241 catcctctgt tgattcggct acgagcagat aatccctgcc attaatacgt gtctgatcca
     3301 gtacgtaaaa ttcagccgtt tcctttccgt catccactgc gaattgtatt ttctccatta
     3361 ccgttcctct ccgtgtcccg gcagccccgc aagataatcc agatatccct gcaaaatgaa
     3421 gatagcagca attttatcta catacttgct cctatcctcc ctgcgtacct taccttccat
     3481 cattgcttta tccgcagcaa ccgttgtcag gcgctcatcc cagagaacga cttccaaacc
     3541 ggtccttcgt atcaaagtct gctggaattc tattgttttt tctccgcgtt ctccgatcga
     3601 cgcattcatg tttttgggaa acccaaggac gatccggtcc acctcatact gcatgatcag
     3661 ctcttcgatt ctggcaagtg tctttctgat tttcttttca gcatcacgtc tgataatttc
     3721 aactccctgg gcagtaatta acagaggatc gcttacggca acccccactg tctttgagcc
     3781 aaaatccaat cccataatac gcatattatt tccatccgtt gtttttgata tactgcgtca
     3841 gcaattcttc cacaagctcg tctctctcca ctttcatgat cagacttctg gcattattat
     3901 gacttgtaat ataagtcggg tcacccgaca tgatataccc aacgatctga ttgaccggat
     3961 tgtatccctt ttcagtcaac gcttcatata cggtggacaa tactaccttt acaccggttt
     4021 ccggttctgt ctgcacattg aaatactgtg tattcccaat attttgcata tactcacttc
     4081 cctgtttcta ctgattgatc tcatcaaaca cttcgcaatt tacatagtta gtattattct
     4141 actccaactc gccggaaaaa acaaggattt catccgtaag catccgttcc gccttaccgc
     4201 ggacgatctt cccgcaaaga tcctcttccc tccgcacggc cgcttttaca tattcttttg
     4261 tataccctgt ctggtaaaac tgcccgttta tctcctgttt ttcttcaaag agcacttcta
     4321 acggccggcc ggcataccat tcgcggtaac gcctgcctct ttttgcagcc tctgctatc
//
LOCUS       DAOSYE010000058         4338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13832_length_4338_cov_2.814616, whole genome shotgun sequence.
ACCESSION   DAOSYE010000058 DAOSYE010000000
VERSION     DAOSYE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13832_length_4338_cov_2.814616"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLU43_01725"
     CDS             <1..547
                     /locus_tag="PLU43_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HOO27157.1"
                     /translation="YPRVKYSIWNGNSDDVIDRLMNGLCDLAVIMEPYNAEGLNALSV
                     YKEPWVAVIPAKHPLAKQKGTTVALQSLAFEELILPSRKSRVAEILSWFDNMEASPVI
                     RCEMAHMMNVYNLTQRNVGIAIFPASISEIPVNDNFVIKEITEPSFTASYVLVWNKAH
                     TLSPLAERFMHMVKETNSCSS"
     gene            complement(544..2319)
                     /locus_tag="PLU43_01730"
     CDS             complement(544..2319)
                     /locus_tag="PLU43_01730"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HOO27158.1"
                     /translation="MYYSSGNYEAFAHPKKPGGVDCKSAYIVGSGLGALAAACFLVRD
                     GQMKGEHIHVLEKEALPGGACDGYEFSDRGFVMRGGREMDNHFECMWDLFRSIPSIET
                     EGISVLDEYYWLNKEDPNYSLMRATVKQGQDAHTDKKFALSDKGAQEIMKLFFTPDEK
                     LYNKRIDEVFDDEVLRSNFWLYWRTMFAFENWHSALEMKLYIRRFVHHIGGLPDFTAL
                     RFTKYNQYESMILPMIAYLKNAKVQFHYETKVENVEFDINGDVKTAKRIICTCRGEQE
                     CIDLTKNDLVFITNGGCVENATLGDQNHPAEFKPELKEGGGWDMWKKIAVQHPSFGHP
                     EKFCSDPEQSNWMSATVTTLDDKIPPYVQKICKRDPFSGKVVTGGIVTAKDSNWLLSW
                     TFNRQPQFRNQPKDQLVGWIYGLFSDQPGNFVKKTMRECTGQEICMEWLYHMGVPTDQ
                     IEELAENSANTVPCMMPYITAFFMPREAGDRPAVVPKGSVNFAFLGQFAETVRDTIFT
                     TEYSIRTAMEAVYTLLNVDRGVPEVWGSMYDIRDLLHATVEMRDGKTIMESELSLKEK
                     LALKEILKKIEGTDIAKLLKEYEVI"
     gene            complement(2484..3059)
                     /locus_tag="PLU43_01735"
     CDS             complement(2484..3059)
                     /locus_tag="PLU43_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOO27159.1"
                     /translation="MARQYTRQMICDTFIKMLNERPLKEITVKDIVTACEINRNTFYY
                     YFTDIYEIIREIFDLELQKVIDEYNNTLSWEECFLKATSYALENKKAVYHIYYSVQRE
                     VLEKYLFDVAGNVMGRYVERMEEGIKASRTDMALITLMYQGALTQIVIYWISNGMKED
                     PEKMILRIGKLFDGNITLSLKRSEQLDGTWI"
     gene            complement(3124..3690)
                     /locus_tag="PLU43_01740"
     CDS             complement(3124..3690)
                     /locus_tag="PLU43_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HOO27160.1"
                     /translation="METKKLFSFNTKTLVATALGAALFMVLGMFVKIPTGIPNTQIQT
                     SYGVCSFFAALFGPIAGALMAFIGHALSDASQYGSVWWSWVIASAVSGFIYGLAFMKL
                     KVSEGIFSGKDIAVFNIIQVIGNALAWIVIAPVLDIVIYAEPANLVFTQGVVAAISNA
                     VSCAVIGTLLLLVYSKTRSKKGSLTKED"
     gene            complement(3799..>4338)
                     /locus_tag="PLU43_01745"
     CDS             complement(3799..>4338)
                     /locus_tag="PLU43_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO27161.1"
                     /translation="LFSLTNRYVMTQEQLLYQVTKVMKYCAVIPLGIIFILATNPSEM
                     AASLNRIGIPYKATFALALTLRYFPDVIRDYHDISIAQQSRGLDMSKKEKLTVRIKNT
                     VNICVPLIFSTLDRIELISNTMDLRGFGMKKNRTWYSEKKLVRNDIVSIVICSCLFLG
                     SLAMTIFVNHSRFYNPFVG"
BASE COUNT         1154 a         1121 c          822 g         1241 t
ORIGIN      
        1 gtatccgcgc gtaaaataca gtatctggaa cggtaactca gacgatgtca tagaccgtct
       61 gatgaatggg ctctgcgatc tggccgtcat tatggaaccc tacaatgcag aaggtctgaa
      121 cgccctgtcc gtatacaaag agccttgggt agccgttatt cctgcaaaac atccgcttgc
      181 aaaacaaaaa ggcacgactg ttgcactgca aagccttgcc tttgaggaac tgatcctgcc
      241 ttcaagaaaa tcgcgtgttg cggaaatatt atcgtggttt gataacatgg aagcgagtcc
      301 tgtgatccgc tgcgaaatgg cacatatgat gaatgtatac aatcttacgc agcgcaatgt
      361 cggtattgcg atctttcctg cgtctatttc cgaaattccc gttaatgaca actttgtcat
      421 caaagagatc acagagccct cctttaccgc ttcctatgtg ctcgtctgga acaaagccca
      481 taccctaagc ccgctcgccg aacgctttat gcatatggta aaagaaacaa attcctgcag
      541 ttcctaaatt acttcatatt ccttcagcaa ttttgcaata tcggttcctt cgatcttctt
      601 aaggatctcc tttagcgcaa gcttttcttt cagactaagc tccgactcca taatagtttt
      661 accatcacgc atttctacgg tcgcatgcag cagatcgcgg atatcataca tgctgcccca
      721 cacttccggc actcccctgt caacgttaag cagcgtatag acagcttcca tagcggtacg
      781 aatggaatat tcggtcgtaa agatcgtatc acggaccgtt tcggcaaact gtcctaaaaa
      841 tgcaaaattc acgcttcctt tcggtacgac cgcagggcgg tctcccgctt cacgcggcat
      901 gaaaaaggca gtgatatacg gcatcatgca tggaaccgta tttgcgctgt tttctgcaag
      961 ttcttcaatc tgatctgtag gcacgcccat atgatacagc cattccatac agatttcctg
     1021 tccggtgcat tcccgcatcg tttttttgac aaaattgccg ggctgatcac tgaacagacc
     1081 atagatccat cctaccagct gatctttcgg ctgattccga aattgtggct gcctgttaaa
     1141 ggtccagctg agcagccagt tgctgtcctt cgccgtcaca atgccgcctg tcacaacttt
     1201 accgctgaac ggatcccgct tgcagatctt ctgcacatac ggcgggatct tatcatccag
     1261 tgtcgtaaca gtcgcagaca tccagttgct ctgttccggg tcagaacaga atttttccgg
     1321 atgaccaaat gacggatgct gcacagcgat cttcttccac atatcccagc cgccgccttc
     1381 ttttaattcc ggtttaaatt cagccgggtg attctgatca ccaagcgttg cattttccac
     1441 acagccgccg ttcgtaataa atacaagatc attttttgtc agatcaatac attcctgttc
     1501 tcctctgcat gtacagatga ttcgttttgc ggtttttacg tcaccgttta tatcaaactc
     1561 cacattctcc acttttgttt cgtaatggaa ctgcaccttt gcatttttca agtatgcgat
     1621 catcggcagg atcatggatt catactgatt atactttgta aaacgaagtg ccgtaaaatc
     1681 cggaaggccg ccgatatgat gcacaaaacg gcgaatatag agcttcattt ccaatgcact
     1741 gtgccagttc tcaaatgcaa acatcgtacg ccagtacagc cagaaattcg aacgcagtac
     1801 ttcatcgtca aatacttcat caattctttt attataaagc ttctcatccg gtgtaaagaa
     1861 tagcttcatg atttcctgcg cccctttgtc cgacagtgca aattttttat cggtatgcgc
     1921 atcctgtccc tgtttgaccg tcgccctcat cagagaataa ttcgggtctt ccttattgag
     1981 ccagtaatat tcgtcaagta cgctgattcc ttctgtttca atagacggga tcgagcggaa
     2041 cagatcccac atacattcaa aatggttatc catctcccgt cctccgcgca tgacaaaccc
     2101 gcggtccgaa aattcatacc cgtcacaggc accgcccgga agtgcttctt tttccagcac
     2161 gtgtatgtgc tctcctttca tctgtccgtc cctgacaaga aaacaggctg ctgccaatgc
     2221 gccaagtccg gagcctacga tataggctga cttgcagtca acgccgcccg gttttttcgg
     2281 atgtgcgaat gcttcataat ttccgctgct gtaatacata tgtattcctc ctttttattg
     2341 ccatcatttt gtttcctgta aatccagtat aatcacagct gttttttaat aaaacggtca
     2401 ctaaaaaagc aatgtccgaa aatcgaacat tgcccttcct ctgtctgttt ttcagacact
     2461 gcggctttta tgcctaaaag cagtcatatc catgtcccgt ccagctgctc gctccgcttt
     2521 aaagacaagg tgatattccc gtcaaacagt tttccaatac gcaggatcat cttttccgga
     2581 tcttctttca ttccattcga gatccaatag atcacgatct gcgtcagcgc tccctggtac
     2641 atcagggtga tcaacgccat atcggtcctg gatgccttaa taccttcttc cattctttcc
     2701 acataccggc ccatcacatt tccggccacg tcaaacagat atttttcaag tacttcccgc
     2761 tggacagaat aataaatatg gtaaaccgct tttttatttt ccaatgcata ggaagttgct
     2821 ttcaaaaaac attcttccca tgaaagggta ttattatatt catcaatgac cttttgcagc
     2881 tccagatcaa aaatttcccg gataatttca tagatatcgg taaaataata ataaaacgta
     2941 tttctgttga tctcacatgc cgtaacgata tcttttaccg taatttcctt cagcggccgt
     3001 tcgttcagca ttttaataaa tgtatcgcat atcatttgcc tggtatattg gcgagccata
     3061 gaaacccctt tcctgatact ttccctgtat tttatagatc tatctgattt ttaataatcc
     3121 cgatcagtct tcctttgtaa ggctgccctt tttgctgcgt gtcttggaat atacgagcag
     3181 cagcagtgta ccgatcactg cgcaggaaac cgcattgctg atagccgcta ctacgccctg
     3241 cgtaaataca aggtttgccg gctccgcata aattacaata tcaagcaccg gtgctattac
     3301 gatccatgca agcgcattgc cgatcacctg gataatatta aataccgcaa tatcttttcc
     3361 ggaaaaaatg ccttcactga cttttaattt cataaatgca agtccataaa taaatcccga
     3421 taccgcactg gcaattaccc agctccacca taccgaacca tactgggatg catcactgag
     3481 cgcatgtccg ataaatgcca taagcgcccc tgcgatcggg ccgaacaagg ccgcaaaaaa
     3541 cgaacatacc ccatagctcg tctgaatctg tgtattcgga atacctgtcg gtatttttac
     3601 gaacatgccg agcaccataa acagggctgc gcctaaagcc gttgcaacaa gagtcttcgt
     3661 gttaaaagaa aaaagttttt tcgtttccat accctctacc tccgagaacc tttctaaaat
     3721 cgtgcgtcct tacggacatt tcattactta ccaaccttga ttatggcaca cttgatttcg
     3781 caagtcaatc aatttccgtc acccgacaaa cggattataa aatctcgaat gattgacaaa
     3841 tatggtcatt gcaagcgaac ccaaaaacag gcacgaacag atcacaatgg aaacaatatc
     3901 gtttctgaca agcttctttt ccgaatacca ggtccggttc tttttcatgc cgaatccacg
     3961 cagatccatc gtattgctga tcagttcaat tctgtcgagt gtggaaaaga tcagaggcac
     4021 acagatattg accgtattct ttatccgcac cgtaagcttc tcttttttag acatgtccag
     4081 tcctctggac tgctgcgcaa tggaaatgtc atgatagtcc cggataacat ccggaaaata
     4141 ccgtaaggtc agtgcaagtg caaacgtcgc tttatatgga ataccgatac gattcaggga
     4201 agctgccatc tcactcggat tcgtcgcaag aataaaaata atcccgagag gaatgaccgc
     4261 acagtatttc attacttttg taacctggta aagaagctgt tcctgtgtca ttacataccg
     4321 gtttgtcaaa gaaaacag
//
LOCUS       DAOSYE010000059         4308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_13961_length_4308_cov_3.073360, whole genome shotgun sequence.
ACCESSION   DAOSYE010000059 DAOSYE010000000
VERSION     DAOSYE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13961_length_4308_cov_3.073360"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3016)
                     /locus_tag="PLU43_01750"
     CDS             complement(<1..3016)
                     /locus_tag="PLU43_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HOO27162.1"
                     /translation="MRMRKRWVKASIAMLALVAMLTESAYSAFATISTEYSSEESASQ
                     ESAPAEESSGSSSTEETSTPAAESTSSETTSSSSTESTEVPVASSTEPAGSSSTEIPA
                     AVQTPETSSSSTEIIPMSENEAGGTSEEEPAEEETAASVNCYEDRIEVTGKQDLTLYI
                     NTDQMNGTDKFSLALEGSALLQYDSLLAGELSKAESGIYYITGLNEEQFRVWVSAVSD
                     GMSVQYSVREDGNPQITLISAPEEEIEKVLSISADGSRISGEGYEDITLSFEAPDLPD
                     EDYFALHVDTAAEASYNGSKLVNGTISSLSNKTTSVRLSDLDNQAFTIYITGENVDAV
                     DALCSVDSVENGAARMIVSLGEDAQKIQSMTASTDGITGSGYEKVELSMEYIEKVLEE
                     AAEQSDSEEDADSEETEAEAQVFQYSLYIETEAENVSVNGQTLDNGKVDYTDSMESVI
                     VDGLDEEAFHIYAVADDAEDTEEPTAISYSVESEESGTAKISFTYTSAEEDVKRVYEY
                     EDSKVKIKVTLQNPEDLPDDAVLVATEIPAGTETYESLSEMITEQKKEEKGAFLSLGQ
                     AVYDIHFTLDGEEIEPENEVEVNIVYKSPALEDSSSNPSNEEASIYHAKENDGGDVVD
                     VEKVAEDVEKSEDGGIAEATFTVEDFSSFVATLDADGKWSNSHGLTYELNKTTNAFTG
                     TKYYNSDRVLGIAGNFHIVAFDDAHLQVHTNGNVLAKELYAGANFGTNNLTDELSYVQ
                     DYAEVNTSNMPSADHTLALGSDATVSFDGEAADATYFKVNGTKIDRPNNIIQDSDTSA
                     NPFIDLNAVKSSISSLSSDLASVADTGVTASFSDQNSRSITLNTPNGAGYYNMTASEL
                     NGYAGNKFDLKGFESGKNGSIIINVNCSGVSTVTMPQSSNVYVDGSMVSASETTEWSA
                     GKVIWNFYNCSGATIYAQSVFGAIIAVDATLYVNKGNGTFIADTVYVTGETHRTDFTG
                     ITTPTSAKLNVHKTFDGTWPDAGFTFKIEAVTD"
     gene            complement(3221..3293)
                     /locus_tag="PLU43_01755"
     tRNA            complement(3221..3293)
                     /locus_tag="PLU43_01755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3258..3260),aa:Thr,seq:cgt)
     gene            complement(3379..>4308)
                     /locus_tag="PLU43_01760"
     CDS             complement(3379..>4308)
                     /locus_tag="PLU43_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27163.1"
                     /translation="KRRWISGIVTTVVMLSVFMLCFFAKSNQMECTNTELWQAYGGRF
                     TVSSNIETGEQYDVSQIMDEFHASFASQNTAIMDNYYFLTKNNGQTYSTLQAAWFQLG
                     NQLFYMFFIVLAIACVFYLLLGNNQKILPCMLALLGSMGVLFFGTNKENNTYFFVELL
                     IMLGCSSLYYMYEAHYPAKEVPAEEILEEASVSPEEAETEDTRTEEEKEAELLRARAL
                     IFIGENEELYYEIKRQENPNGILKTPEYYMNRKAEESMSCEHEFVKKEVKFIENPLPL
                     PKKHVAKTMEYGFEPDENMDFDYDIKEDDDFDI"
BASE COUNT         1126 a         1094 c          768 g         1320 t
ORIGIN      
        1 cgtcggttac cgcttcgatc ttaaacgtaa agcccgcatc cggccatgtg ccgtcaaatg
       61 tcttgtgaac attgagtttt gcactggttg gtgttgtaat gccggtaaag tctgttctgt
      121 gtgtttcacc tgttacataa acagtatccg caataaatgt accattgcct ttattgacat
      181 ataaagtagc atcaactgct ataattgctc caaatacaga ctgtgcataa atcgtagcgc
      241 cggaacagtt atagaaattc catattactt ttcctgcgga ccattctgtt gtctcactgg
      301 cagacaccat gctgccgtcc acataaacat tggaactctg cggcatagtc actgttgata
      361 cgccgctgca gtttacatta ataataatgg aaccgttctt cccgctctca aatcctttca
      421 gatcaaattt attgccggca tatccattta attcggaagc cgtcatatta taataacctg
      481 ccccatttgg cgtgttcagc gtaatggaac ggctattctg atcgctgaag ctggctgtaa
      541 cacccgtatc tgccacgctt gcaagatcac tggaaagaga agatatggaa cttttaacag
      601 cattcagatc gataaacgga tttgccgacg tatcggaatc ctgaataatg ttatttggtc
      661 tgtctatttt tgttccgtta actttaaaat aagtcgcatc cgctgcctca ccgtcaaaag
      721 aaaccgtagc atcggagccc agtgcaagcg tatgatctgc agatggcata ttggaggtat
      781 tcacctctgc ataatcctgt acataagata actcatctgt gagattattc gttccgaaat
      841 tagcaccggc gtataattct tttgcaagta cattaccgtt tgtatgtact tgcaaatgag
      901 catcgtcaaa tgcgacaata tggaaattgc cggcaattcc aagaactctg tcggaattat
      961 aatacttcgt gccggtaaat gcattcgtcg tcttattcag ttcatatgta aggccatgag
     1021 aattagacca cttcccgtcg gcgtccaatg tcgcaacaaa actggaaaag tcctcaacgg
     1081 taaaggttgc ttccgcaata ccgccatcct cgctcttttc tacatcttcc gctacctttt
     1141 ccacgtcgac cacgtctccg ccgtcatttt ctttcgcatg gtatatggaa gcctcttcat
     1201 tggaaggatt ggatgaactg tcctcaagtg ccggactctt atatacgata ttaacttcca
     1261 cttcattttc cggctctatc tcttctccgt ctaatgtaaa atgaatatca taaacagcct
     1321 gccctaatga caggaaggct cctttttctt ctttcttctg ttccgtaatc atttcagaaa
     1381 gagattcata tgtttctgtt ccggcaggta tttccgtagc gaccaaaaca gcatcatccg
     1441 gcagatcttc cggattctgc agggtgactt taattttcac tttgctgtct tcatattcat
     1501 aaacccgttt tacatcttct tcagctgatg tataggtaaa ggatatcttt gctgttccgc
     1561 tttcctcgga ttccacagaa taagaaattg ccgtcggttc ttccgtatct tcagcatcat
     1621 ccgcaaccgc ataaatatgg aaagcttctt catcaagtcc gtcaacgatc acgctctcca
     1681 tggaatcagt ataatccact ttaccattat caagtgtctg accgtttacc gacacattct
     1741 ccgcttctgt ttcaatatac agagaatact ggaatacctg tgcttcggct tccgtttctt
     1801 cactatccgc atcctcctct gaatcagact gctctgcagc ctcttcaagg actttttcta
     1861 tatattccat ggacagctcg accttttcat aaccggaccc cgtaataccg tcggttgatg
     1921 ctgtcatgga ctgtattttc tgggcatctt ctcccagcga tacgatcata cgtgccgcac
     1981 cgttttctac cgaatccacg gagcagagcg catccaccgc atccacattt tcgcctgtaa
     2041 tataaatagt aaatgcctgg ttatctaaat cagaaagacg aacacttgtt gttttattgg
     2101 agagtgatga gatcgtacca tttaccagtt tgcttccatt ataagaagct tctgccgccg
     2161 tatccacatg cagggcaaaa taatcctcat ctggcaggtc cggtgcttcg aagcttaacg
     2221 taatatcttc atatccttca ccactaatac gacttccgtc agccgatatc gagagtactt
     2281 tttctatttc ttcttccggc gctgaaatca gggtgatctg cggatttcca tcttcccgga
     2341 cagagtactg cacactcatg ccatccgaaa cagcagatac ccatacgcgg aactgttctt
     2401 cattcaatcc cgtaatataa tagataccgc tttccgcctt tgacagttct cctgcaagca
     2461 gggaatcata ctgcagcagc gcactgcctt ccaaagccag actgaattta tccgttccat
     2521 tcatctgatc cgtattgata tacaatgtca gatcctgctt ccctgtcacc tcgatcctgt
     2581 cctcataaca gttcacagat gctgcagttt cttcctctgc cggttcctct tccgaagttc
     2641 cccctgcttc attttcggac atcggtataa tctccgtact cgaactcgat gtttccggtg
     2701 tctgcaccgc agccggtatt tctgtcgacg acgaacctgc aggctctgtc gatgacgcca
     2761 ccggcacttc cgtactttct gtcgaagatg aactcgtagt ctctgacgac gtactctcag
     2821 cagcaggtgt gctggtttcc tccgttgatg atgatccgga actttcctct gccggagcac
     2881 tttcctgcga agcactctct tcagacgaat attcggtcga tatagttgca aacgcactgt
     2941 atgccgactc cgtcagcatg gctaccagtg cgagcattgc aattgaagcc tttacccatc
     3001 tcttcctcat tctcataatc tttctccatc cctttcattt tctaaaaaac aaatatcacg
     3061 gaactattca tatatgatac attttgcaat attaacaaaa aaattttcct ttttatatat
     3121 taaagactac ttttttccgg aaaaagggtc agtattataa aaacattttt ttatataaag
     3181 caaaagtgca gcccataaag actgcacttt tacaaataaa agctcccgag gggattcgaa
     3241 cccctgacct acgcattacg aatgcgtcgc tctaccagct gagccacgga agcctggaat
     3301 caaacgacta cctttgctat tatattaaat ttcatttcat tttgcaacaa caattttatt
     3361 ctttttatgt ttttatattt atatatcgaa atcgtcatct tccttaatat cataatcaaa
     3421 atccatattt tcgtccggct caaacccata ctccattgtt ttggccacat gctttttagg
     3481 aagcggcagc ggattttcaa taaatttcac ttcttttttc acaaactcat gctcacaact
     3541 catgctttcc tcagcctttc tgttcatgta atattccgga gtcttcagaa taccattcgg
     3601 gttctcctgt ctctttatct cataatacag ttcctcattt tcaccaataa agataagggc
     3661 acgggcacgc aaaagctcgg cctccttttc ttcctccgtc cgtgtatctt ccgtctcggc
     3721 ttcttcaggc gaaacggacg cttcttctaa tatttcttcc gcgggcactt ctttagcagg
     3781 ataatgagcc tcatacatgt aatagagact ggaacaaccg agcatgatca gcagttccac
     3841 aaagaaatat gtattatttt ctttattggt cccaaaaaac agcactccca tcgagccgag
     3901 cagtgcaagc atgcatggca gtattttttg attatttccc aaaagcagat aaaatacaca
     3961 ggcaatcgca agcacaataa agaacatata aaagagctga tttccaagct gaaaccaggc
     4021 cgcctgcaag gtagaatatg tctgtccgtt attcttcgta agaaaataat aattgtccat
     4081 gatcgctgtg ttctgactgg caaacgaagc atgaaattca tccatgatct gactgacatc
     4141 atactgctct ccggtttcga tattagatga gactgtaaac cttccgccat atgcctgcca
     4201 taattccgtg ttcgtacatt ccatctgatt gctctttgca aaaaaacaga gcataaaaac
     4261 ggaaagcatc acaactgtcg ttacaatccc gctgatccag cgtctttt
//
LOCUS       DAOSYE010000060         4278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_14098_length_4278_cov_3.352119, whole genome shotgun sequence.
ACCESSION   DAOSYE010000060 DAOSYE010000000
VERSION     DAOSYE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14098_length_4278_cov_3.352119"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1468)
                     /locus_tag="PLU43_01765"
     CDS             complement(176..1468)
                     /locus_tag="PLU43_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO27164.1"
                     /translation="MDENEFQEQAPMIQTSEEQLEELQAKARKKGVKEGVLISLLSVF
                     VIAFICMIVLLIIKVRDGSMFFSAWNSTSDSSDSIVTDEVEEKINVLKSAIDEYYLDE
                     VDDEALQDGMYKGLLEGLDDPYSVYYTKKEYDDMMDSSSGIYYGIGAYLQQDVNTMEI
                     KVTKPIPGTPAEEAGLQPDDIIKEVDGEDISKEDINVVVSKIKGKEDTKVTLGIQREG
                     EDDLLQFEITRQRVEITTVESKMLEDDIGYIWIYEFDDVTRDQFMSAFDDLNEQGMQS
                     LILDLRDNPGGNLDVVVDIADRLLPEGLIVYTQNKYGQKDEFTSDAEHYFDKPLVVLV
                     NGNSASASEILAGAIKDYKLGTLLGTTTYGKGIVQQIVPLSDGTGVKLTVSKYYTPNG
                     YNIHGVGIEPDETLEFDSDAYEKDQTDNQLNRAIELLK"
     gene            complement(1513..2733)
                     /locus_tag="PLU43_01770"
     CDS             complement(1513..2733)
                     /locus_tag="PLU43_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO27165.1"
                     /translation="MTRKFRWVGFACAIMAVFLAAAEVPLIVHASLTNDYIKESEESK
                     KQAEQNRDTLKSGLTDIKELLNSLESAKGNLESYITQLDADLSDISTKIDSLNEMITE
                     KEADIETTQAALSEAKQMEEEQYNAMKQRIRFMYEKGSSSYVEMLFSSGSFVDFLNKA
                     EFINKISEYDREMLDQYIETKNQIAEAEAELEEEQEDLEDTKADLAVEQDAVETLISS
                     KEEEITVYESDISNKEQAVEEYEAEIAAQDQLIKDLEASILAEKKRLLEENKSAIVYD
                     GGQFAWPAPQYTRISDDYGYRIHPILGVQQFHNGVDMAAPSGSPILAAYDGEVVAASY
                     SSTMGNYIMINHGDGLYTIYMHASSLGVSTGQVVARGEQIACVGSTGRSTGPHLHFGV
                     RLNGSYVSPWNYLQ"
     gene            complement(2793..3692)
                     /gene="ftsX"
                     /locus_tag="PLU43_01775"
     CDS             complement(2793..3692)
                     /gene="ftsX"
                     /locus_tag="PLU43_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOO27166.1"
                     /translation="MRISTFFYTFKQGFKNIFRNKVFSLASIATIAACIFLFGLFYAV
                     VINVQYSVKAAQSGVAVTVFFDEGISDEQITEIGEEIKQRPEVSEVDYVSSDEAWEEF
                     KVDYLGEYADGYEGDNPLEGSDNYEIYMNDVAKQAQLVSYLESLDGVREVNRSDVTAD
                     ILSNINTLIGYISLGIIVILLAVSLFLISNTVAMGIAVRKEEISIMKLIGATDFVVRS
                     PFVIEGILIGIIGSALPLVAIYYLYTNVMIYAVEKFSALSDILKFMSVEEMFSKLMPI
                     ALILGVGIGFFGSFSTVRKHLKV"
     gene            complement(3682..>4278)
                     /locus_tag="PLU43_01780"
     CDS             complement(3682..>4278)
                     /locus_tag="PLU43_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27167.1"
                     /translation="VFIVGDSGSGKSTLIKLLLRELTPTHGRVIVNNYDLSRLRRRKI
                     ARFRRNIGVVFQDFRLLKDRNVYENVAFAQRVIEVPTRQIKKNVPAMLSLVGLAEKYK
                     AKPKQLSGGEQQRVALARALVNNPPILLADEPTGNLDPRNSWEIMKLLEEINKRGTTV
                     LVVTHNREIVNAMRKRVITVKKGVIISDEEKGEYIDED"
BASE COUNT         1114 a         1149 c          746 g         1269 t
ORIGIN      
        1 gtaaggagca tgaagcttaa attcgttcat cataagatcc tttctgaatt gtctatccgc
       61 attatatcaa aaaaagaaac aaaagtacag cacaaatcga atgtttgttc tgtacctttg
      121 ttttctattt ttcgttccga aatacggaac ggacgatcta tataaaatat aatctctatt
      181 tcagcagctc gatcgcacgg ttaagctggt tgtccgtctg atctttttca taggcatccg
      241 aatcgaattc cagcgtttca tccggttcaa taccgactcc gtgaatattg tatccgtttg
      301 gtgtgtaata tttcgatacg gtcagcttta caccggtccc gtcactgagc ggcacgatct
      361 gctgtacgat tccttttcca tacgtcgttg tgccaagcag tgtgccgagt ttataatctt
      421 taatggcacc ggccagtatt tcagaagcac tcgcactgtt tccgttgaca agcacaacaa
      481 gcggcttgtc gaaataatgt tccgcatctg acgtaaattc atccttctgt ccgtatttat
      541 tctgtgtata tacgatcagt ccctccggca gcagtctgtc cgcaatatct acgaccacat
      601 ccagattccc tcccgggtta tcacgcagat ccaggatcag tgactgcata ccctgttcat
      661 tcagatcatc aaatgccgac ataaactgat ctctcgtaac atcatcaaat tcatagatcc
      721 agatatatcc gatatcatcc tcgagcattt tagattccac ggtcgtaatt tcaacacgct
      781 gtcttgtgat ctcaaactgt aaaagatcat cttcaccctc tctttgaata ccaagcgtca
      841 cctttgtgtc ttcttttccc ttgattttcg acacgacaac attgatatct tcctttgaaa
      901 tatcttctcc gtctacttcc ttgataatat catccggctg cagcccggct tcctccgcag
      961 gtgtccccgg aatcggcttc gttactttga tctccatcgt attgacatcc tgctgcagat
     1021 aagcgccaat accataataa ataccggaag aactatccat catatcgtca tattcttttt
     1081 tcgtataata tacggaatat ggatcatcca gcccttccag aagtcctttg tacataccgt
     1141 cctgcagtgc ttcatcatct acttcatcca gataatattc atcgatcgca gacttcaaaa
     1201 cattgatctt ttcttctact tcatccgtta caatactgtc ggaagaatca gaggtcgaat
     1261 tccatgcgga aaaaaacata gatccatctc taacctttat gatcagcaaa acaatcatac
     1321 atataaaggc aattacgaat acagacagca gggaaatcaa aaccccctct ttcacgccct
     1381 tttttcgtgc ctttgcctgc agttcttcca actgctcttc cgatgtctgt atcatcggtg
     1441 cctgttcctg aaattcattc tcatccattc tgcttcctcg gttcttttat taataaagta
     1501 ttccctcata atttactgta aataattcca cggactcaca tagcttccat tcaggcgtac
     1561 accaaagtgc agatgcggtc ctgtgcttct tcctgtcgaa ccaacgcagg cgatctgttc
     1621 ccctcttgct actacctgtc ctgtagatac cccaagggaa gacgcatgca tatagatcgt
     1681 atacagaccg tctccatgat tgatcataat ataattgccc atagtggaag aataagaagc
     1741 cgctacgacc tcaccgtcat aggcggcaag tatcggcgag ccggaagggg ctgccatatc
     1801 cacaccgtta tgaaactgct gcacgccgag tatcgggtgg atacggtatc cgtaatcatc
     1861 tgaaattctt gtatattgag gcgccggcca tgcgaactga ccgccgtcat atacgatcgc
     1921 actcttattc tcttcaagca gccgcttttt ttctgcaaga atcgatgctt ccagatcttt
     1981 gatcagctga tcctgtgctg cgatttcagc ctcgtattct tctaccgcct gctctttatt
     2041 gctgatgtcg gactcatata ccgtgatctc ctcttctttg gaggaaatca gtgtttccac
     2101 ggcatcctgt tctaccgcaa gatcagcttt tgtatcctca agatcttcct gctcttcctc
     2161 aagctccgct tccgcttccg ctatctgatt tttggtttca atatactggt cgagcatttc
     2221 tctgtcatat tccgatatct tattgataaa ttccgcttta tttaaaaaat ccacgaaaga
     2281 gccggaagaa aacagcattt ccacataaga actgctgccc ttttcataca tgaagcgtat
     2341 cctttgtttc atggcattat actgctcctc ttccatctgt tttgcttcag aaagagcagc
     2401 ctgcgtcgtc tcgatatccg cttctttttc cgtgatcatt tcattcagac tgtcgatttt
     2461 cgtactgata tcagacagat ccgcatccag ctgtgtaata taactttcca gattgccctt
     2521 ggcgctttca agactgttta aaagctcctt gatatcggtc agtcctgatt tcagcgtatc
     2581 ccgattctgt tcggcctgtt tctttgattc ttcactttcc ttgatataat cattggtcag
     2641 ggatgcatgc acaataagcg gcacttctgc cgctgccaga aaaacagcca tgattgcaca
     2701 ggcaaaaccc acccatcgaa acttccttgt cattttccag ccccttaatc aactacgcgg
     2761 tccgctcccc agcagaccct gtttcattaa cactaaacct ttaaatgttt ccgtacagtc
     2821 gaaaaacttc cgaaaaaacc gattcccact ccaagaatca atgcaatcgg catcagcttg
     2881 gaaaacatct cctcgaccga cataaatttc agaatatccg acagtgcact gaatttttct
     2941 accgcatata tcataacatt cgtatataga taataaatcg cgacaagcgg cagtgcggaa
     3001 ccgataatac cgatcagaat cccttcgatc acaaacggcg agcgtacgac aaaatccgtt
     3061 gcgccgatca gcttcatgat cgatatttct tcttttctga ccgcaattcc cattgctacc
     3121 gtattactga tcaaaaataa ggataccgca agcaggatca caataatacc aagtgaaata
     3181 tatccgatca gggtattgat attggaaagg atatctgccg taacatcgga acggtttacc
     3241 tccctgaccc cgtccagaga ttccagataa gatacaagct gtgcctgttt ggctacgtca
     3301 ttcatataga tttcgtaatt gtcggagcct tccagcggat tgtctccttc ataaccgtcc
     3361 gcatactctc caagataatc cactttaaac tcttcccatg cctcgtccga agaaacatag
     3421 tcaacctcag acacttccgg acgctgtttg atttcctctc caatttccgt gatctgttca
     3481 tcggaaattc cctcgtcaaa aaatacggtg accgccacac ccgactgcgc cgctttcacg
     3541 ctgtactgca cattgatgac aacagcataa aacagcccga acaaaaaaat acacgccgca
     3601 atcgtagcaa tcgaagcaag agaaaacact ttattacgaa aaatattttt aaaaccctgc
     3661 ttaaatgtat agaaaaacgt actaatcctc atctatatat tcaccctttt cctcatcgct
     3721 gataataacg cctttcttta ccgttataac cctcttgcgc atcgcattta cgatttcacg
     3781 gttatgcgtt actacaagca ctgttgtccc tcttttattg atttcctcca gcagcttcat
     3841 gatctcccat gaattccgcg ggtccagatt cccggtcggc tcatccgcca gaagaatcgg
     3901 cggattattc acaagtgccc ttgcaagggc aaccctctgc tgctcaccgc ccgacagctg
     3961 cttcggtttg gctttatact tttccgccag tcccacaagc gataacatgg ccggcacgtt
     4021 ttttttgatc tgccttgtcg gcacttctat gacacgctgt gcaaacgcca cattttcata
     4081 gacattccgg tccttaagca aacggaagtc ctgaaaaacg acccctatat ttcttctgaa
     4141 cctggcaatt ttccggcgtc ttaaccttga cagatcataa ttgttaacga tcaccctgcc
     4201 atgtgtcggc gtcagttcac gaagaagcag cttgatcagc gttgatttac cggaaccgct
     4261 gtccccgaca ataaaaac
//
LOCUS       DAOSYE010000061         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_14277_length_4242_cov_3.128015, whole genome shotgun sequence.
ACCESSION   DAOSYE010000061 DAOSYE010000000
VERSION     DAOSYE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14277_length_4242_cov_3.128015"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PLU43_01785"
     CDS             <1..138
                     /locus_tag="PLU43_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding oxidoreductase"
                     /protein_id="HOO27168.1"
                     /translation="VNFDEAMKRQQLYKTVEGRAYLKEKEGDTHRANHGRGCDNHGGD
                     E"
     gene            135..1523
                     /gene="gltA"
                     /locus_tag="PLU43_01790"
     CDS             135..1523
                     /gene="gltA"
                     /locus_tag="PLU43_01790"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOO27169.1"
                     /translation="MMDVLKKVPVREQDAKVRATNFEEVCLGYNEAEAMEEAARCINC
                     KNAKCIEGCPVAINIPAFIHEVKEGNFEEASKVIGQSSALPAVCGRVCPQESQCEGKC
                     IRGIKGEPVSIGKLERFVADWSREHHIKPEGAEKKNGHKVAVIGSGPAGLTCAGDLAK
                     RGYDVTIFEALHEPGGVLVYGIPEFRLPKQKVVAEEIENVKTLGVKIETNVVIGKSTT
                     IDELMEEEGFEAVFIGSGAGLPKFMGIPGENANGVFSANEYLTRNNLMKAFDSSYDTP
                     IRAGEKVAVVGGGNVAMDAARTALRLGAEVTVVYRRSEEELPARVEEVHHAKEEGIKF
                     NLLTNPTEILVNEDGWVKGLKCVRMELGAPDESGRRRPVVVEGSEFELAVDTVIMSLG
                     TSPNPLISSTTKGLDTNKYKCIVADEESGKTSKDGVFAGGDAVTGAATVILAMGAGKA
                     AAKGIDEYLSNK"
     gene            1631..2488
                     /locus_tag="PLU43_01795"
     CDS             1631..2488
                     /locus_tag="PLU43_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27170.1"
                     /translation="MPWCPNCKNEYKDGITVCSDCGAALIQSLEELNEQEIVKYTFGT
                     KNEMEQLYEFMKFGGLSYVELKCEENAEEAENYFIEIEKKEEKEAERLSSIYYKELNK
                     KEPSDGKTEIEEQNDRTSLPEDTAPVPFVKASERAENYRSSAYALLLIGILGIIALLL
                     IAAGIISISIADNIKVISFITMLLLFLVFTFMGIKALKDAKKFAADSEKEDQLSADIR
                     SWFLEQYTKEVLDEAADVCYTPDIPEEMKYFKRINYMKAAVIKQFGDLDASFLDKNTE
                     DLYTEIYEH"
     gene            2478..2957
                     /gene="rlmH"
                     /locus_tag="PLU43_01800"
     CDS             2478..2957
                     /gene="rlmH"
                     /locus_tag="PLU43_01800"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOO27171.1"
                     /translation="MNIDLICVGKIKESYLKAAILEYEKRLSRYCRLKIIETADEKTP
                     DGQSSVLDNKIRQTEAERILKHIKKESCVISLEIGGEMLDSVELSKKIETLGINGTNN
                     IQFIIGGSLGLHPSVSEMADLKLSFSKMTFPHQLMRVILLEQVYRSYRIMHNEPYHK"
     gene            2991..>4242
                     /locus_tag="PLU43_01805"
     CDS             2991..>4242
                     /locus_tag="PLU43_01805"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOO27172.1"
                     /translation="MLMVDLIRKKREGHTLSKEEIEYMIQGYTKGEIPDYQMSAMMMA
                     IFFRGMDDEETVHLTMAMMHSGDELDLSGINGIKVDKHSTGGVGDKTSLVLTPMVASL
                     GVPVAKMSGRGLGHTGGTIDKLESFPGFHTSITEEQFLHNVNTIKIAIMGQTADLAPA
                     DKLLYALRDVTATVDNMSLIASSIMSKKLAAGADAIVLDVKCGSGAFMKTEADAFELA
                     EKMTSIGNMAGRKTVAVVSDMDQPLGFAVGNALEVEEAIATLKGNGPADLLELCLTLG
                     SLMIIMAGKAKEENEAREMLTAAIADGRALKKLSEFIQAQGGRAEDVYDTSSLPKASI
                     LYELKAEQDGYIGKIFSEDVGNASLLLGGGRRSKTDVIDLAVGLILKKKVGDRIKTGE
                     TLAVLYANDENKLAEARERLAGAIT"
BASE COUNT         1351 a          735 c         1120 g         1036 t
ORIGIN      
        1 gtgaattttg atgaagccat gaagcgccag cagttatata agaccgtgga aggcagagct
       61 tatctgaaag aaaaagaagg agacacccat cgtgccaatc atggcagggg atgcgacaac
      121 cacggaggtg acgaataatg gacgtattaa aaaaagtacc tgtcagagag caggatgcga
      181 aggtgcgcgc aacaaatttt gaagaagtgt gtcttggata taacgaagcg gaagcaatgg
      241 aagaggcagc cagatgtatt aactgcaaaa atgcaaaatg tatcgaaggc tgtccggtag
      301 caatcaatat tccggcattt atacatgaag ttaaggaagg caattttgaa gaggccagca
      361 aagtcatcgg tcagtcttcg gcactgccgg cagtctgcgg acgtgtgtgc ccgcaggaga
      421 gccagtgtga gggcaaatgt atcagaggaa tcaagggaga gccggtatcg atcggtaaac
      481 tggagcgttt tgtggcagac tggtcaagag agcatcatat taaaccggaa ggtgcagaaa
      541 agaagaacgg ccataaagtt gccgtgatcg gttccgggcc tgccggattg acgtgtgccg
      601 gcgatcttgc aaaacgggga tatgatgtga ctatttttga agcgcttcat gaaccgggcg
      661 gtgtgctcgt ttatgggatt cctgaattcc gtcttccaaa gcagaaagtc gttgcggaag
      721 aaatcgaaaa tgtaaagact ctcggcgtaa aaattgaaac aaatgttgta attggaaaat
      781 ctacaacgat cgatgaattg atggaggaag aaggatttga ggcagtcttt attggttccg
      841 gtgccggact tccgaaattc atgggtattc ctggagaaaa tgcaaacggt gtattttctg
      901 ccaacgaata tctgacaaga aacaatttaa tgaaagcgtt cgacagttcc tatgatacgc
      961 cgattcgggc aggtgaaaag gttgctgtcg taggcggcgg caatgtggct atggatgctg
     1021 caaggacagc cttaagactt ggtgcggaag tcactgttgt atatcggaga agtgaagaag
     1081 aacttcctgc cagagtggaa gaagtacatc atgcgaaaga agaaggtatc aaatttaatt
     1141 tgctgacaaa tccgacggaa attcttgtta atgaagatgg ctgggtaaaa ggcttgaagt
     1201 gtgtgagaat ggaacttggt gcgccggatg aatccggcag aagaagacct gttgttgttg
     1261 aaggctctga atttgagctt gcagttgata cggttatcat gtcgcttggt acatctccga
     1321 atcctttgat ttcttccaca acaaagggac tggatacgaa caaatataaa tgtattgttg
     1381 cggatgaaga atccggaaag acttcaaaag atggtgtatt tgccggcggc gatgccgtaa
     1441 cgggtgctgc tacggtcatt ttggccatgg gtgccggaaa agctgcagct aagggaattg
     1501 atgaatattt aagcaataaa tagacctgtt tgacagactg aggcacgttt gaaacgtgcc
     1561 tcaatttgaa aacttattat ccggcacaga caacaggcgg gataattttg aattttaggg
     1621 ggaagaagac atgccatggt gtccgaattg taaaaatgaa tataaggatg gaattactgt
     1681 ctgcagtgat tgcggagctg cactgatcca gtcgcttgag gagctgaatg aacaggaaat
     1741 cgtaaaatat acgtttggga caaaaaatga aatggaacag ctctatgaat ttatgaaatt
     1801 cggcggtttg tcctatgtgg agctgaaatg tgaagaaaac gcagaagaag cagaaaatta
     1861 ttttattgaa atagaaaaaa aagaagaaaa agaagctgaa aggctttctt ccatttatta
     1921 taaagaacta aataaaaaag aaccttctga cggaaagacc gaaatagaag agcagaacga
     1981 caggacatct ttgccggagg atacggcgcc tgtcccattt gttaaagcat ccgaaagagc
     2041 ggaaaattac aggtcctctg catacgcact gcttttgatc ggtattcttg ggatcattgc
     2101 tctgcttttg attgccgcag gtatcatttc gatctccatt gccgacaata tcaaagttat
     2161 ttcttttatt acgatgctgc ttttatttct tgtttttacg tttatgggga tcaaagcgct
     2221 gaaagatgcc aaaaaatttg cggcagactc tgagaaagaa gatcagctgt ctgcagatat
     2281 tcgttcctgg tttctggaac aatatacgaa agaagtactg gatgaagcgg cagatgtatg
     2341 ttatacaccg gatattccgg aagaaatgaa gtattttaaa agaataaact atatgaaagc
     2401 agcagtcata aaacaattcg gcgatcttga tgcatccttt ctcgataaga atacggagga
     2461 tctgtacacg gaaatatatg aacattgatt taatatgcgt tggaaaaata aaagaaagct
     2521 atttaaaagc ggcgattttg gaatatgaaa aacggctttc aagatattgc aggttaaaaa
     2581 tcattgaaac cgcggatgaa aaaacgcctg acggacagag cagtgttttg gataataaga
     2641 tcagacaaac ggaagctgaa cggatactga agcatataaa aaaagaatcc tgtgtaattt
     2701 cacttgaaat cggcggggaa atgcttgatt ccgtggaatt gtcaaaaaaa atagagacgc
     2761 ttggaataaa cggaacaaac aatattcagt ttattatcgg gggttctctc ggactgcatc
     2821 catccgtttc agaaatggcg gatttaaaac tgagtttttc caaaatgacg tttccgcatc
     2881 agctgatgcg ggtgatctta ctggaacagg tttaccggag ctaccggatc atgcacaatg
     2941 aaccatatca caaataatat ttcagctatg caacagggag gtaccgaatc atgctgatgg
     3001 tcgatttgat cagaaaaaaa agagaaggtc atacactttc aaaagaagaa atagagtata
     3061 tgattcaggg atatacaaaa ggagaaatac cggattatca gatgtcggca atgatgatgg
     3121 ctattttctt ccggggaatg gatgatgaag agaccgtcca tcttacgatg gcaatgatgc
     3181 acagcggaga cgagctggac ctgtccggta tcaacggcat aaaagtagat aagcacagta
     3241 ccggaggcgt tggtgacaaa acatcacttg ttttgacgcc gatggtggcc agtcttggtg
     3301 taccggtcgc caaaatgagc ggcagaggac ttgggcatac gggaggtacg atcgataagc
     3361 ttgaaagctt tccgggattc catacttcta ttacggaaga gcagtttctt cataatgtca
     3421 atacgatcaa aattgcaatc atgggtcaga ctgcagacct tgctccggca gacaagcttc
     3481 tttatgcact tagggatgta acagcaactg ttgacaatat gtcattgatc gcaagttcga
     3541 tcatgagcaa aaaattggcc gcaggcgcgg acgcgatcgt acttgatgta aagtgcggaa
     3601 gcggcgcatt tatgaaaaca gaagcggatg catttgaact ggctgaaaaa atgacttcta
     3661 ttggcaatat ggcgggccgg aaaacagttg cagtcgtatc tgacatggat cagccgcttg
     3721 ggtttgcagt cggaaatgcc ttggaggtag aagaagcgat cgccacattg aaggggaacg
     3781 gtccggcaga tctgctggag ctgtgtctga cacttggttc tttgatgatc atcatggccg
     3841 ggaaggcaaa agaagaaaac gaagccagag aaatgctgac agcggcaatt gcagatggca
     3901 gggctttaaa aaagctgtct gaatttattc aggcacaggg aggcagggca gaagatgtct
     3961 atgatacatc ctcccttcca aaagcatcca ttctgtatga actgaaagca gaacaggacg
     4021 gttatattgg gaaaattttc agtgaggatg tcggaaacgc gtctcttttg ctcggaggcg
     4081 gcagaagaag caaaacggat gtgattgatc tggcggttgg tcttattctg aaaaagaaag
     4141 tcggtgacag gattaaaaca ggagaaacac tggctgtttt gtatgcgaat gatgaaaata
     4201 agcttgcaga ggcaagggaa cgtctggccg gtgcaataac aa
//
LOCUS       DAOSYE010000062         4241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_14280_length_4241_cov_3.287148, whole genome shotgun sequence.
ACCESSION   DAOSYE010000062 DAOSYE010000000
VERSION     DAOSYE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14280_length_4241_cov_3.287148"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLU43_01810"
     CDS             complement(<1..379)
                     /locus_tag="PLU43_01810"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222462.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOO27173.1"
                     /translation="MQKGMTMTQKILAAHAGLPYVEAGQLIEAELDLVLGNDITTPVA
                     IREMNRINNNQVFNKDKIALVMDHFVPNKDIKSAENCKCVREFACKHEISHYYDVGDM
                     GIEHALLPEKGLTVAGDAIIGADS"
     gene            545..1447
                     /locus_tag="PLU43_01815"
     CDS             545..1447
                     /locus_tag="PLU43_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO27174.1"
                     /translation="MENNLSLYKIFCAAAESGNISAAAKKLYISQPAVSKAIAKLEQN
                     INATLFIRSSKGVSLTYEGQLLFDSVKSAFDFISAGEAKLQNIVEFGGGHIKFGASTT
                     LCKYILLPYLKDFMKQNPQIQISVESQSTYQTLELLQAGKIDIGLIGKPNVAKIQNGT
                     LTYTPIADIEDIFVATKDYIESQHTTDLLRHSTLLMLNKENMTRQYIDHYLYANQIEP
                     QQLMEITSMDLLIDFAKIGLGTACVIREFVKSELKSQQLVEVPLKSPIPKRSVGFVYT
                     GGTLASVASNRFVKFIHADAPVSF"
     gene            complement(1538..2188)
                     /locus_tag="PLU43_01820"
     CDS             complement(1538..2188)
                     /locus_tag="PLU43_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27175.1"
                     /translation="MKNTIKKMLMAATGFCLCTALFLLVPQKTYAYPCTIDLINEAKA
                     QKSAAEAELLAAQNNKIAADAAVTAAKASGASQLVLQQAYDNASNAANLVRAAVDKVN
                     NAQAFIENAQERLAAEDEYEANVVKWNNQVTIASAKEAATTAKEIADTAASLITIVRQ
                     NIASLTSQLAVSPSLQTQIDQLNASLPSLEADYAANLADANEKAAITDYLEKTLVH"
     gene            complement(2263..3687)
                     /gene="miaB"
                     /locus_tag="PLU43_01825"
     CDS             complement(2263..3687)
                     /gene="miaB"
                     /locus_tag="PLU43_01825"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOO27176.1"
                     /translation="MNFNRTIPPETEPERQYYFMEKAGAYVKAKETETGRKLTFHVTT
                     FGCQMNARDSEKLSGILKTIGYIEEEDEKKADFVLFNTCTVRDNANQRLYGRLGMLNG
                     KKYKKTDRMIGLCGCMMQEPSVIEKIRKSYRFVDMIFGTHNIYKLAELLCTKFETGGM
                     VIDIWKDTDKIVENIPNDRKYPFKSGINIMFGCNNFCSYCIVPYVRGRERSRNPADIL
                     QEIRRLSDDGVKEIMLLGQNVNSYGKNLAHPISFAALLKEVEQIDGIERIRFMTSHPK
                     DLSDELIMVMKESKKICRHLHLPLQSGSTKILTAMNRHYTKEQYVDLALRIRREIPEI
                     AITTDIIVGFPGETREDIDETIDVIKKVQFDGAFTFLYSKRTGTPAAAMENQVSAQFA
                     KEQFDRVLHAVREMSEKMAGRYEGSIEKVLVEGINEQDRTLLTGRLDNNLLVHFKGNE
                     VQIGSFVSVELKECRGFYYVGKQI"
     gene            complement(3684..>4241)
                     /locus_tag="PLU43_01830"
     CDS             complement(3684..>4241)
                     /locus_tag="PLU43_01830"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOO27177.1"
                     /translation="KIRNRILQIRNALSLLECTEKSAQIMHMVKQQEEYTTSKLLFLY
                     ASYQNEVMTGTLILEALREGKRIALPCAAVTNGVPQLDFYEITDISQLADGYRGIPEP
                     DKKNKAVRMVETLPDLLLLPGVAFDRQRNRIGYGMGFYDHYFTRQKQVRAIAVAYECQ
                     VVSQIPVSGFDYRPDKLITEERIYQ"
BASE COUNT         1100 a          989 c          835 g         1317 t
ORIGIN      
        1 gggaatccgc cccgataatg gcgtctcctg caaccgtcag tcctttttcc gggagcagag
       61 cgtgttcaat acccatatcc cccacatcat aatagtggct gatttcatgt ttgcaggcaa
      121 actccctgac acatttacag ttttccgctg acttgatatc tttattcgga acaaaatgat
      181 ccataacgag tgcgattttg tctttattaa aaacctggtt attgtttatc cgattcattt
      241 cccggatggc gaccggtgtt gtaatgtcat ttccaagaac gagatccagc tctgcttcga
      301 tcaactgtcc cgcttccaca tacggcagcc ccgcatgtgc cgctaaaatt ttctgtgtca
      361 tagtcatacc cttttgcatt ttatctttcc tcctgattat tttactggtt aatatttatt
      421 tgctgattac aagaagtata gcataccctg ttccatgaat aaaatatata tttttcatat
      481 ttacgataac ccattgttat ggtatgatta ctctatcaat aggtaggtaa aggagctgtt
      541 atttatggaa aataatctgt cgctttataa aatattttgt gctgccgccg aatcaggaaa
      601 tatctccgca gccgcaaaaa aactttatat cagccagccg gccgtcagta aagcaattgc
      661 aaaactggaa caaaatatta atgcgactct gtttataaga agttcaaaag gcgtttctct
      721 gacttatgaa ggacagctgc tttttgacag tgtaaaatcc gcatttgatt ttatctccgc
      781 aggagaagca aaattacaga atattgtgga attcggcggc ggacatatca aattcggtgc
      841 cagcacaaca ctgtgcaaat atattctgct tccttatctg aaagatttta tgaaacagaa
      901 tcctcagata cagatatccg ttgaaagtca gtctacatat cagacgctgg aattgctgca
      961 ggccggtaaa attgatattg gtctgatcgg aaagcctaat gttgcaaaaa tacagaacgg
     1021 tacgcttacc tatacaccga ttgcagatat tgaggatatc tttgtggcaa caaaggacta
     1081 tatcgagtct cagcatacga cggatctgct gcgtcattcc acacttttga tgctgaacaa
     1141 ggaaaacatg acacgtcagt atatcgacca ttatctgtat gcaaatcaga tagaaccaca
     1201 gcagctgatg gaaatcacct ccatggatct tttgatcgat tttgctaaga tcggccttgg
     1261 tacagcgtgc gtcatcaggg aatttgtaaa atcagaacta aaatcacagc agcttgtcga
     1321 ggtcccgctg aaatcgccga tcccgaagcg ttctgtcggg tttgtttata cgggcggcac
     1381 actggcctca gtcgcctcga acagatttgt gaaatttata catgcagatg cgcctgtttc
     1441 tttttaaaca ataccgcaaa accatgtacc tgcaaaaagc aggcgaccat ttacgatctc
     1501 ctgctttttt cgttatttca aatacgattc agtatcatta atgaaccagt gtcttttcca
     1561 ggtagtccgt gatggctgct ttttcatttg catctgcaag atttgctgca taatcggctt
     1621 caagtgaagg aagcgacgca ttcagttggt cgatctgtgt ctgcagggaa ggactgactg
     1681 caagctgtga tgtcagagaa gcaatattct gcctgacaat cgtgatcagg gacgcggcag
     1741 tatcagcaat ttcttttgcg gtggttgccg cttcttttgc actggcgatc gtaacctgat
     1801 tgttccactt tacgacattt gcttcatatt catcttcggc tgcaagacgc tcctgtgcat
     1861 tttcgataaa ggcctgtgca ttatttacct tatctactgc cgcgcgaaca agattcgccg
     1921 cattggaggc attgtcataa gcctgctgca atacaagctg ggaagcgccg gatgcttttg
     1981 ccgccgttac cgccgcatct gctgcaatct tattattctg tgctgcaaga agctctgctt
     2041 cggccgcact tttttgtgct ttggcttcat tgatcagatc gatcgtacat ggatacgcat
     2101 atgtcttctg cggaaccagc aaaaataatg ccgtacataa acaaaagccg gtggctgcca
     2161 ttaacatctt ctttattgta ttcttcatag gtgttctcct ttcaaaggct tctatatgtt
     2221 attataacaa aataatacgg tttgtcacta taattttata attcagatct gttttccaac
     2281 ataataaaat cccctgcatt cttttaattc aacggaaaca aaagaaccga tctgaacttc
     2341 attcccttta aaatgtacaa gaagattatt gtcaaggcgt ccggtcagta atgtcctgtc
     2401 ctgttcgttt atcccttcaa ccagtacttt ttcaatactg ccttcatatc gtccggccat
     2461 tttttcagac atttcccgga cagcatgcag cacacggtca aactgttctt ttgcgaactg
     2521 tgcagatacc tgattttcca ttgctgctgc cggcgttccg gtccgtttgg aatataggaa
     2581 agtaaaagca ccgtcaaact gcactttttt gatcacatcg atcgtttcat caatatcttc
     2641 ccttgtttct cccggaaagc cgacaataat atccgtggta attgcaattt caggtatttc
     2701 ccgtctgata cggagcgcca ggtcaacata ttgttccttt gtataatgtc tgttcattgc
     2761 agtcagtatt tttgtacttc cggactgcag cggcaggtgc agatgcctgc atatcttttt
     2821 ggattctttc ataaccatga tcaattcatc cgacagatcc tttggatgtg acgtcatgaa
     2881 ccggatccgt tcgatcccgt cgatctgttc gacttctttg agaagcgctg caaaactgat
     2941 tggatgtgca agatttttcc cataggaatt aacgttctga ccaagcagca tgatctcttt
     3001 cacaccgtca tctgaaagcc gtctgatctc ctgcagaata tccgcagggt tccggctgcg
     3061 ttctcttccg cgcacatacg gcacaataca gtaactgcag aaattattgc agccaaacat
     3121 gatattgata ccggatttaa acggatattt ccgatcgttt ggaatgtttt caacaatttt
     3181 atccgtgtcc ttccaaatat cgattaccat gccgcctgtt tcaaattttg tacatagaag
     3241 ctcggcaagc ttataaatat tatgcgtccc aaagatcatg tcaacaaatc ggtagctttt
     3301 cctgattttt tcgatcacgg acggttcctg catcatacag ccgcaaagac cgatcatccg
     3361 atctgttttt ttatattttt tgccgttcag catgccaagc ctgccataca gacgctgatt
     3421 ggcattgtcc ctgaccgtac aggtattgaa taaaacaaaa tcggcttttt tttcatcttc
     3481 ttcctctata taaccgatcg tcttcaaaat tccggacagt ttttcagagt cccgcgcatt
     3541 catctgacag ccaaaggtcg tcacatgaaa ggtcagtttc ctccctgttt cagtttcttt
     3601 tgcctttaca tatgcaccgg ctttttccat aaaataatac tgacgttcag gctctgtttc
     3661 cggtggtatt gttctattga aattcattgg tagatccttt cttctgttat cagtttatca
     3721 ggtctgtaat caaagcctga aacagggatc tgtgaaacga cctggcattc ataagcaacg
     3781 gcgatcgccc ggacctgttt ctgacgcgtg aaataatggt cataaaatcc cattccatag
     3841 ccaatacggt tcctttgcct gtcaaatgca acacctggca gcagcaaaag atccggcagg
     3901 gtttcgacca tgcgcactgc cttatttttt ttgtcaggct ccggaatgcc cctgtatccg
     3961 tccgcgagct gtgaaatatc tgtgatttca taaaaatcaa gctgcggaac gccatttgta
     4021 acggcagcac acggcagagc aattcgtttc ccttcccgca gagcttccag aatcagcgta
     4081 cctgtcatca cttcattttg gtaggaagca tacagaaaca gcagtttgga agttgtatat
     4141 tcttcctgct gttttaccat atgcatgatc tgcgcactct tttccgtgca ctccaaaaga
     4201 ctgagtgcat ttctgatctg cagtatcctg ttcctgattt t
//
LOCUS       DAOSYE010000063         4215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_14423_length_4215_cov_4.756971, whole genome shotgun sequence.
ACCESSION   DAOSYE010000063 DAOSYE010000000
VERSION     DAOSYE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_4215_cov_4.756971"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..2434
                     /locus_tag="PLU43_01835"
     CDS             359..2434
                     /locus_tag="PLU43_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27178.1"
                     /translation="MKDLKMRKKLLILATVVGFIPMIVVGLLSYILADRELENAVDTT
                     NSVYALLAEAQLDTYFSERKGNADTLSSADNLVLNMETYSDAGASESEKAAALANMET
                     YLAAERDAYNYPDIFITDTSGMSILATVYKNELEGADLSIRTYVQSALQGTQNWSELF
                     YSSFLDDNAMVLSSPIYNKDGSQVIGTVNLLINQAKMDEIIHKGADILGTTGDAYLID
                     TNGLLLSNTRLGDYVEGAALNQTIDTKATEVLSDEVAAGNTDFTYTGIYDNYMGNAVC
                     GSLGVVKIGDTNAGLIIEIDESEAFAGVYTLRNASIVIVLFFYIIAILMLILIASSIT
                     KPLAAVVGNAELLAAYDLTQEVEKKHVERKDEIGAIANTVQKVVLNLRSMMQEVSKES
                     SQLAALSEELTAISEQNSSAIMEVANTVNDIAAGAASQAENTLSGSKNLEKLSALIEE
                     EGSHIEKMEDQIIKVSKLIEEGLAVSAQLSAKAKQNTEASNVVFESIRKTNESTVKIG
                     EASSLIASIAQQTNLLSLNAAIEAARAGEHGKGFAVVAGEIGKLAEQSAVNTKYIDEL
                     LKNLKTDVDAAVAKMEETELIVNEQETSVKTTIDKYNEIAGAMRNVENSIGVLEKASR
                     TIEENRAEVQKNIKSLSDVAESNAAATEEVSASVEEQSASMEEISSSTEHLSQQALEL
                     QNLVDKFKV"
     gene            2631..3884
                     /locus_tag="PLU43_01840"
     CDS             2631..3884
                     /locus_tag="PLU43_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27179.1"
                     /translation="MKIRMKIKWKITIVNLLLVLGTAALIEVDSYFGFSLLLVIPFVA
                     LVGFIISFIIGGYIAKGYEAIGRDLSLMSSGDFTIKLDEKAGKRNDEIGIASRALSDM
                     ETGTSRMVLNLKNEVSQIDHLLSESVAYIKEVYTEVEGISATTEQLSAGMQETAASTE
                     EMNATSHEIEAGVQNVAQKAQLGAGSAKEIKERVEKLIKETETSRNNAISLFENTNVK
                     LRNSIEKSEAVNQIQVLSNTILEIASQTNLLALNAAIEAARAGEAGKGFAVVADEIGK
                     LAEDSKNTVTKIQDVSEEVTEVVESLVKDSQEILEFVDKEVIKDYEMMKNTGIQYNDD
                     ADLMSGMMEELSATAEELHASMLNMINVIEEITRAATEGAQGTTMIAEKSTSIVTKTD
                     QVVKDAETNHKSVEKLSKFLERYKI"
     gene            complement(4015..>4215)
                     /locus_tag="PLU43_01845"
     CDS             complement(4015..>4215)
                     /locus_tag="PLU43_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO27180.1"
                     /translation="VTGLKKITPHKLRSTYGTSLYRETGDIYLVADVLGHKDVNTTKK
                     HYAALEDDQRRKAANVVKLREP"
BASE COUNT         1406 a          700 c         1019 g         1090 t
ORIGIN      
        1 cttcgggaaa atagtttcga agcaatggag tttttcttaa caattcaaaa tcaactgcat
       61 ttgtcagaag aagacagcgc tgtcctttcc agaaatataa aattttttga gtacgatgag
      121 gcaattaaaa cgattgaaaa tgcgatgaca gtataattaa aatatataaa tcatccgtca
      181 ggatctaatc cagatggatg attttttatt ttgtatatat atttaaaacc gtgacatatg
      241 acactagtgc atacaaaatg tttgtaatag actgaaattg tttaaagaaa gcatgtatta
      301 tatttaaaat atcattttaa ataaaaggtc aaaatatttg acagaaagga aagagcgtat
      361 gaaagattta aaaatgagaa aaaaattatt gattttagcg actgtagtag gcttcattcc
      421 gatgatcgta gtaggcctgc tgtcgtacat tctggcagac agagagctgg aaaacgccgt
      481 tgacacgaca aactctgtat atgctctgct ggcggaagca cagctagata cttatttttc
      541 ggaacggaag ggaaacgcag atacgctttc ctcagcggac aatcttgtac tgaatatgga
      601 gacctatagt gacgcagggg catcggaaag tgaaaaagct gctgcacttg ccaatatgga
      661 aacctatctg gcagctgaaa gggatgctta taattatccg gatattttca taaccgatac
      721 gagtggcatg agtattcttg ctactgtata taagaatgaa ttggaagggg ccgatctgag
      781 tatacgtact tatgtccaaa gtgcactgca gggaacacag aactggtccg aactttttta
      841 ttcaagcttc ttagatgata atgcaatggt gctctccagt ccaatctata ataaagatgg
      901 cagtcaggtg attggaacgg tcaatttgct gatcaatcag gctaaaatgg atgagatcat
      961 tcataaaggt gcggatattc ttggaacaac aggagatgcc tatctgattg atacaaatgg
     1021 tcttctgctg tcaaacacaa ggcttggaga ttatgtggaa ggagctgcgc tcaatcagac
     1081 gatcgataca aaagcaacgg aagttttatc ggatgaggtc gcggctggaa atacggattt
     1141 tacctataca ggaatatatg ataattatat gggcaatgca gtatgcggct ctcttggtgt
     1201 tgtaaagatc ggagatacaa atgcgggtct gatcatcgag atcgatgaat cggaagcatt
     1261 tgcaggggtt tatacactgc gaaacgcatc gatcgtcatc gtgctgttct tttatataat
     1321 tgcaattctg atgctgattc tgattgcgtc ctccatcaca aagccgctgg ctgctgttgt
     1381 tggaaatgcg gagcttttgg cagcatatga tctgactcag gaagtagaga agaaacacgt
     1441 tgaaaggaag gacgaaatcg gggcgattgc aaataccgtt cagaaggtcg tcttaaattt
     1501 aagatcgatg atgcaggagg tttccaaaga atccagtcag ctggcagcct tgtctgaaga
     1561 gctgaccgcg atctctgagc agaattcttc ggcgatcatg gaagtggcca atacggtgaa
     1621 tgatattgcg gcgggagcag caagccaggc ggaaaatacg ctgtccggtt ccaaaaatct
     1681 tgagaagtta agcgccctga tcgaagagga aggttctcat atcgagaaga tggaagatca
     1741 gattataaag gtaagcaagc ttattgaaga agggctggcg gtttctgcac aattatccgc
     1801 aaaagcaaag cagaatacgg aagcatcgaa cgttgtattt gagagtattc ggaagacaaa
     1861 tgaaagtacg gtcaagatcg gggaagcaag cagtctgatc gcctctatcg cccagcagac
     1921 caatctgctg tcactgaatg cggcgattga agcagcaaga gcaggagagc atggaaaagg
     1981 ctttgctgtt gtagccgggg agattggaaa gctggcagag cagtctgcag taaatacaaa
     2041 atatattgat gaattgctga aaaatctgaa gacagatgtg gatgcggcag ttgcgaaaat
     2101 ggaagagacc gaactaatcg tcaatgagca ggagacaagt gtgaagacga cgatcgataa
     2161 atacaatgag atcgcaggcg cgatgagaaa tgtggaaaat tcaatcggcg tactggagaa
     2221 agcatccagg acaatagaag agaaccgggc tgaagtccag aagaacatca agagcctttc
     2281 tgatgtggca gaaagcaatg cggcggcgac tgaagaggta tcggcttccg tagaggagca
     2341 gtcggcctcc atggaagaga tctcaagctc tacggaacat ctgtctcagc aggcattgga
     2401 gctgcagaat ctggttgata aattcaaagt gtaggattta gaaaacgatt ttctgcaatt
     2461 gatattaaaa attgacgatc gattttccta tatacgatgg tattggttga atattattaa
     2521 aatatcagcg aaaacatgac aaaaaaagga cctataaata aaaaaaaata atatataata
     2581 ataaaaaagc aacccagtcg gattgctttt ttatgggatg aaaaatttgg atgaaaatta
     2641 gaatgaagat caaatggaaa ataacgattg tgaatttatt acttgttctg ggtactgcag
     2701 ctttaattga agtggatagt tattttgggt tttctctgct gcttgtaatt ccttttgtag
     2761 cactggtcgg ctttattatt tcatttatta tcgggggata tatcgcaaaa ggatacgaag
     2821 cgatcggaag agatctatct ttgatgtcaa gcggggattt cacaattaaa ttagatgaaa
     2881 aagcaggaaa acgaaatgat gagatcggaa ttgcatcacg ggcacttagc gatatggaga
     2941 ccggaacatc caggatggtt ctgaatctca aaaatgaagt ctctcaaatt gatcatctcc
     3001 tgtcagagtc tgtggcatat ataaaggaag tttatacaga agttgaaggg atttctgcta
     3061 ctacagagca attatccgcc ggtatgcagg aaacggccgc atcaacggaa gaaatgaatg
     3121 cgacctctca tgaaatagag gcaggcgtgc agaatgtggc acaaaaagcc cagctcgggg
     3181 caggatctgc aaaagaaata aaggaacggg tggagaaact gatcaaagag actgaaacat
     3241 ccagaaataa tgcgatctca ctatttgaaa atacaaacgt aaagcttcgg aattcgatcg
     3301 aaaaatcaga agcggtaaat caaattcagg tattgtcgaa tacaattctt gaaattgcgt
     3361 cacaaactaa tttgctcgct ttaaacgctg ccatagaggc tgcaagagcc ggagaggccg
     3421 gaaaaggatt tgccgtagtt gcagatgaaa taggaaaact ggcggaagac tcaaagaata
     3481 cggtaactaa aattcaggat gtatctgaag aagttaccga agtagtagaa tctttagtaa
     3541 aagactctca ggaaatactc gaattcgtag ataaagaagt gatcaaggac tatgagatga
     3601 tgaaaaatac tggaattcag tataatgatg acgcggattt aatgtcaggc atgatggagg
     3661 agctttccgc aactgcagaa gaactgcatg cgtccatgct gaatatgatc aatgttattg
     3721 aagaaatcac ccgtgcggca actgaagggg cgcagggcac aacgatgatt gccgaaaaat
     3781 ccaccagcat tgtgacaaag acagatcagg tcgtaaaaga tgcggaaaca aaccataaaa
     3841 gtgtggaaaa attaagtaag tttcttgaaa gatataaaat ataaaataaa aagaaaaaag
     3901 aggagtggtt tattcctctt cttttttatt gcaggagatg ttgaaataat tatattttta
     3961 tgaatcattg tgttgaaata tatacttatc gtggtaaaat gaattcttta ttatttacgg
     4021 ttcccgcagc tttacgacat tagcggcttt acggcgctga tcgtcttcaa gggcagcata
     4081 gtgcttctta gtcgtattga cgtctttgtg tccgaggaca tcagctacca gataaatatc
     4141 accggtttcc ctataaagac ttgtaccgta agtgcttcgc agcttatggg gtgttatttt
     4201 cttaagccct gtgac
//
LOCUS       DAOSYE010000064         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_14815_length_4143_cov_3.473092, whole genome shotgun sequence.
ACCESSION   DAOSYE010000064 DAOSYE010000000
VERSION     DAOSYE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14815_length_4143_cov_3.473092"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLU43_01850"
     CDS             <1..423
                     /locus_tag="PLU43_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO27181.1"
                     /translation="DKVIRTSLDGFYNMTKPILKKMVRQKRGAVVSIASLSGLTGNRG
                     QANYSASKAGLIGLTKSTARELASRRITVNAIAPGFIETEMTGVLPDKVKERMLAAIP
                     LGRIGCAKDVAAAVLFLASEQAAYITGQVLGVDGGMAM"
     gene            480..1721
                     /gene="fabF"
                     /locus_tag="PLU43_01855"
     CDS             480..1721
                     /gene="fabF"
                     /locus_tag="PLU43_01855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOO27182.1"
                     /translation="MKRRVAVTGLGAITPIGLSVEEFWDSVKQQKIGFDRITRFDPTE
                     YKCSLAAEVKGFDGKNYMDFKSAKRMELFSQYAVAAAKEAMEDAGIDMEKEDAYRAGC
                     AIGSGIGSLQAMEKEHTKLVEKGPNKLNPLFIPMMISNMAAGNVSIAFGLKGKSINVV
                     TACATGTNCIGEAFRTIQYGDADIMLAGGTESAVTPVGIAGFTALTALSTESDPARCS
                     LPFDKDRSGFVMGEGAAVVVLEELEHARKRGAKIYAEVTGYGCSSDAFHITSPAEDGD
                     GAAMAMKNAMADAGVCPEEIFYINAHGTGTHHNDLFETRAIKKAFGAHAYKLYINSTK
                     SMIGHLLGAAGAIEFVTCVMEMKESYIHATAGYTTPDEEMDLNYCREPYYEKVRHALT
                     NSLGFGGHNTSLLVSEYEKER"
     gene            1721..2161
                     /gene="fabZ"
                     /locus_tag="PLU43_01860"
     CDS             1721..2161
                     /gene="fabZ"
                     /locus_tag="PLU43_01860"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOO27183.1"
                     /translation="MPILTAEQIMEIIPHRQPFMLLDTIEELEPGKRALAKKCVTYNE
                     PYFAGHFPKEPVMPGVLIIEAMAQAGAAAMLSLEEMKGRTAYFAGINSARFKRKVIPG
                     DVLMLDVEIIKIKGPIGIGRGSASVDGKLAASAEILFAIGKEEN"
     gene            2163..3941
                     /locus_tag="PLU43_01865"
     CDS             2163..3941
                     /locus_tag="PLU43_01865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOO27184.1"
                     /translation="MWAVKRSRKKYLTLKKLTGMNRNRIRQEEEDGTAEEKAQGADNI
                     AEQGEDIVLCRHCGASLSKQETVTNKYVCKKCGYYFRVRTNNRIKMIADAGSFEEWFT
                     DIEQTDPLGFPGYQEKLAKVREKTGQDEGVTVGYARIQGEPVVLGVCDSRFFMGSMGH
                     AYGEKITCAFEKAVELKLPVILFCCSGGARMQEGIISLMQMEKTAAAVRKHSDAGLLY
                     IPVLTDPTTGGVTASFAMLGDIILAEPGALIGFAGPRVIEKTIGEKLPEGFQSAEFQL
                     EHGFVDMVVERTHLKGTLYRLVKFYHNITSFHGFFPGKADHAEPDELAREQMAKASSH
                     SAWAKVRAARRVDRPSALDYMELIFDDFLELHGDRYFRDDPAIVGGAAYLDGQPVTVI
                     GIQKGKDINECMRRNYGMCSPEGYRKALRLMKLAEKFHHPVITFINTSGAYCGKEAEE
                     NGQGEAIARNLYEMSALKVPIITMMIGEGGSGGALALAVGNEVWMMENATYSILSPEG
                     FASILWKDAGRVEEAAEVMKLTAEDLKKLYVIEKIIPEFGGADSLTLPSIAMYMKRSL
                     KEYLIMHACEEDKDFAKERCERFRKF"
     gene            3966..>4143
                     /locus_tag="PLU43_01870"
     CDS             3966..>4143
                     /locus_tag="PLU43_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOO27185.1"
                     /translation="MTGRICGTGSCFPKLRVTNDDLSKLMDTSDEWIRSRTGIEARYI
                     AVGETTTDMAAIAAK"
BASE COUNT         1213 a          802 c         1236 g          892 t
ORIGIN      
        1 gacaaggtga tccgaaccag ccttgacggc ttttacaata tgacaaagcc gattctgaaa
       61 aaaatggttc ggcagaaacg gggggctgtt gtttccatcg catcgttgtc cggcctgaca
      121 ggaaaccggg gacaggccaa ttacagtgca tcaaaggccg gactcatcgg cctgacaaaa
      181 agtacggcaa gagaacttgc ctcccgcagg attaccgtca atgcgattgc gcccggattt
      241 attgagacgg aaatgacagg tgtgcttccg gataaagtga aggagcggat gctcgcggcg
      301 ataccgcttg gcaggatcgg atgcgcaaaa gatgttgcgg ctgccgtgct gtttcttgcg
      361 tcggagcagg cagcctatat taccggacag gtacttgggg ttgacggcgg tatggcaatg
      421 tagccggtat gcggcagaac tttatttatg aagacgaaga agcagagcag gaggcagata
      481 tgaaacgaag agtagcggta acaggactcg gggcgatcac cccgatcgga ctgagcgtgg
      541 aggaattttg ggattccgta aaacagcaga aaattggatt tgacaggatc acaagatttg
      601 atccgacaga atataagtgc agtctggcag cagaagtaaa aggttttgac ggcaaaaatt
      661 acatggattt caagtcggca aaaagaatgg agctgttcag tcagtatgcg gttgcggcgg
      721 caaaggaagc gatggaagac gcagggatcg acatggaaaa agaggatgcg tatcgggccg
      781 gctgtgcgat cgggtccggt atcggcagtc tgcaggcaat ggaaaaagaa catacaaagc
      841 ttgtggaaaa aggaccgaat aagctgaatc cgctgtttat tccgatgatg atatcgaata
      901 tggcggcagg aaacgtatcc attgcatttg gattaaaagg aaagagtatc aatgttgtga
      961 cagcctgcgc gacaggaacg aattgtatcg gagaggcgtt tcgtacaatc cagtacggag
     1021 atgcggacat tatgctggca ggcggaacgg aaagcgccgt gacaccggta ggaatcgcag
     1081 gttttactgc tttaacggca ttgtccacgg aatcagatcc ggcacgctgt tcccttccgt
     1141 ttgacaaaga ccgcagtggg tttgtaatgg gagaaggtgc ggcagtcgtt gtgctggaag
     1201 agctggaaca tgccagaaaa cgcggagcaa agatctatgc ggaggtgacc ggatatggct
     1261 gctcctcgga tgcctttcat atcacatccc ctgctgaaga cggggacgga gcggcaatgg
     1321 caatgaaaaa cgcgatggca gatgcgggtg tctgtccgga agaaatattt tatatcaatg
     1381 cgcatggtac cggcacacat cataatgatc tgttcgagac gagagcaatc aaaaaagcat
     1441 tcggcgcaca tgcatataag ctttatatca attcgacaaa atccatgatc ggtcatctgc
     1501 tcggagctgc gggtgcgatc gaatttgtga catgcgttat ggaaatgaag gagtcctata
     1561 ttcatgccac ggcaggctat acaacgccgg atgaagaaat ggatctcaat tactgcaggg
     1621 aaccttatta tgaaaaagtc agacatgcgc tgacgaattc actcggtttc ggagggcata
     1681 atacgtctct gcttgtttca gaatacgaga aggagcgctg atgccgatac ttaccgcaga
     1741 gcagatcatg gagatcattc cgcacaggca gccgtttatg ctgcttgata cgatagagga
     1801 gctggagcct ggaaaaagag cgttggcaaa aaaatgcgtg acttataatg agccgtattt
     1861 tgccggtcat tttccaaagg agccggtcat gccgggcgta ctgatcatag aagcaatggc
     1921 acaggcgggt gcggctgcaa tgctttcact ggaggagatg aaaggaagga ccgcatattt
     1981 tgcggggatc aattccgcaa gatttaaaag aaaggtcatt ccgggggacg tactgatgct
     2041 ggacgtggaa attataaaga tcaaaggacc gatcgggatc ggacggggca gtgccagtgt
     2101 ggacggaaag cttgcagcga gtgcggagat tctgtttgcg atcggaaaag aggaaaactg
     2161 atatgtgggc tgtaaagcga agcaggaaga aatacctgac attgaaaaaa ctgacgggaa
     2221 tgaacaggaa ccgcatccgc caggaggaag aagacggcac ggcggaagaa aaagcgcagg
     2281 gagcggacaa tattgccgaa cagggagaag atattgtttt atgcagacac tgcggggcgt
     2341 ctttgtccaa acaggaaaca gttacgaata aatatgtatg taaaaagtgc ggttattatt
     2401 tcagggtaag aacaaataac aggatcaaaa tgattgcgga tgccggaagc tttgaggaat
     2461 ggtttaccga tatagaacag actgatccac tggggtttcc gggttatcag gaaaaactgg
     2521 ccaaagtacg ggaaaaaaca ggacaggatg aaggggtgac ggtcggttat gccagaatac
     2581 agggagaacc ggttgtgctc ggtgtctgcg attccagatt ttttatggga agcatgggtc
     2641 atgcctatgg tgaaaagatc acctgtgcat ttgagaaagc agtggaatta aagctgccgg
     2701 ttattttatt ctgctgctcc ggcggggcaa ggatgcagga aggaatcatt tctctgatgc
     2761 agatggaaaa aacagcagca gctgtcagaa aacacagtga tgcagggctg ctttacattc
     2821 cggtgctgac ggatccgacg acaggagggg tcacggcaag ctttgccatg ctcggagata
     2881 taatactggc ggaaccgggg gcactgatcg gatttgcagg tccccgtgtt atagaaaaga
     2941 cgataggaga aaaactgccg gaaggctttc aaagtgcgga gttccagctg gaacacgggt
     3001 ttgtggatat ggtcgtggag agaactcatc tgaagggtac cctgtaccgg cttgtgaaat
     3061 tttatcataa tattacaagc tttcacggat tttttcctgg aaaagccgat cacgcggaac
     3121 cggacgaact ggcacgggaa cagatggcca aagcatcaag ccacagtgca tgggcaaaag
     3181 tcagggcggc ccgcagagtg gacagaccat ctgcacttga ctatatggaa ctgatattcg
     3241 acgattttct ggaacttcat ggagatcgtt atttcaggga tgatccggca attgtcggcg
     3301 gcgcggccta tctggacgga cagcctgtca cggtgatcgg tattcaaaag gggaaggata
     3361 tcaacgagtg catgaggaga aattacggaa tgtgttcacc ggaagggtat cggaaagcac
     3421 tgcgtctgat gaagcttgct gaaaaatttc atcaccctgt tatcacgttt atcaatacat
     3481 ccggtgcata ctgcggaaaa gaagccgaag agaacgggca gggagaagcg atcgcgcgta
     3541 atctgtatga aatgtccgca ttaaaagtgc cgattattac tatgatgatc ggagaaggcg
     3601 gaagcggcgg tgccctggcg cttgctgtgg gaaatgaagt gtggatgatg gaaaatgcga
     3661 cgtattccat attatcgccg gaaggctttg catcgatctt atggaaggat gcaggccgtg
     3721 tggaagaagc ggctgaagtc atgaagctga ctgcagaaga tctgaaaaag ctgtatgtca
     3781 tagaaaaaat cataccggaa ttcggcggtg cggacagcct gacactgcct tctatcgcga
     3841 tgtatatgaa acggagcctg aaagaatatc tgatcatgca tgcatgtgaa gaagacaagg
     3901 attttgcaaa agagcgctgc gaaagatttc gtaaatttta aatacggcgg gtacggagga
     3961 taaaaatgac tggtagaata tgcggaacgg ggagctgttt tccgaagctt cgtgtgacaa
     4021 acgatgattt aagtaaactg atggatacat ccgacgagtg gatcagaagc cgtacgggga
     4081 tagaagccag atatattgcg gtcggggaga ccacgacgga tatggctgcc attgcagcaa
     4141 aaa
//
LOCUS       DAOSYE010000065         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15050_length_4100_cov_3.633375, whole genome shotgun sequence.
ACCESSION   DAOSYE010000065 DAOSYE010000000
VERSION     DAOSYE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15050_length_4100_cov_3.633375"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1026
                     /locus_tag="PLU43_01875"
     CDS             361..1026
                     /locus_tag="PLU43_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOO27186.1"
                     /translation="MKAKISHALHFLLGVSKDTRLWGAGVIIGAVLLSISFFIEPESR
                     DLLIQDKIFCADEKATASQTEENFNISSILSRNTEIAAAGVSADMDYIENETDLVLDE
                     DVSISLDDFDALCKIVYSEAGCEDMEGQIMIANVVLNRTKSDIFPDDIQKVIMQPGQF
                     EPVTCGSYFYADPSKMTREAVMSALNGEDLSNGALYFQKSSSKVWDDHVYLFRHGSHS
                     FYK"
     assembly_gap    1108..1117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1118..1876)
                     /locus_tag="PLU43_01880"
     CDS             complement(<1118..1876)
                     /locus_tag="PLU43_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27187.1"
                     /translation="MTLAGAYIATAKNGTIYYRSSFTYKNKHISLGSFESEELAHQAY
                     LDASRLISDSASILSYQADTHILSFDKYVSIVNFRDNNVYFKNPIYLEKHYFLYYLTE
                     ELVYKFDIDDLFYYAGHKISKRGGHLFVADYGMQISLPSRYGIRSHAVMDRGYRFING
                     DCFDFRYENIEIINQFYGVTKICQKNKTRYKAQIHINGMITVGTYDTETAAAVAYNKA
                     VDLVKKKFPERRYNQNYIDTMAPSQYASLYIRIRV"
     gene            2023..3006
                     /locus_tag="PLU43_01885"
     CDS             2023..3006
                     /locus_tag="PLU43_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOO27188.1"
                     /translation="MARSSNQKLKLLYLLKILTEHTNEQHPMNTAALIKALEAYGISA
                     ERKSIYSDIEELIHFGIDIEKSGGRRNAGYYVAGREFELAELKLLVDAVQSSKFITGK
                     KTDSLIRKLGLLTDKYQAAKLKRQVFVANRTKTQNESIFYNIDDIYRAIQDGKEISFQ
                     YFEWSINKELLLKKNGEAYIAGPLALIWDDENYYLVAYDKKAGFRKHFRVDKMKNIQV
                     LESRPDIMDRSFDIAAYSKKMFGMFGGETDDVILRCPNGKIGILLDRFGKEITVRQLD
                     GDFCRVRVQVVVSEQFFGWLCAIGTDISIVAPAQIKEQYRMHLKKILDNYE"
     gene            3180..>4100
                     /gene="nrdD"
                     /locus_tag="PLU43_01890"
     CDS             3180..>4100
                     /gene="nrdD"
                     /locus_tag="PLU43_01890"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO27189.1"
                     /translation="MYKPDRDVRMVKKDGTLEAFNVQKVIDAVGKSAYRALTKFTDQE
                     KEYICQKVIDRVNELGADEIPIPIMHNIVESALEEVKPIVAKSYRDYRNYKQDFVRML
                     DDVYKKSQSIMYIGDKENSNTDSALVSTKRSLIFNQLNKELYQKFFMTTEEIQACRDG
                     YIYVHDMSARRDTMNCCLFDVQSVLTGGFEMGNIWYNEPKTLDVAFDVIGDIVLSAAS
                     QQYGGFTVPSVDYILEPYAEKSYRKYLDKYERLGIDREKAEAEAYADVTREFEQGFQG
                     WEYKFNTVASSRGDYPFITVTAGTGTGRFQK"
BASE COUNT         1267 a          654 c          993 g         1176 t
ORIGIN      
        1 ggaaatatgc agtcggaaca gcagcaggaa gatacggatg ccggaattac aacagattat
       61 ccatatcagt ttacaatgtg gcagtatgca gagtctggta caataaacgg aatcagcggc
      121 tcagtgaatc tggatatttg ctttgtgaac tatgatgaaa aataagcaga gataaaaaaa
      181 gcacatggca atttctaatg atttttcaca aaaacagatg aaaaactttg tgaaattcat
      241 agaaacataa tcttttgtga taaaaaatca ataatttttg ataatttgtg gtatactact
      301 ctttgtcagt ttctttcaat gcttggggag gcattgattg agaaggaagg agtttattgt
      361 atgaaagcaa aaatcagcca tgcacttcac ttcctgctcg gtgtgtcgaa agatacccgt
      421 ttatggggtg ccggcgtaat aatcggagcg gttttgctta gtatcagctt tttcattgaa
      481 ccggagagca gggatctttt gatccaggac aagatctttt gtgcagatga gaaggcaaca
      541 gcatcgcaga cggaagaaaa ttttaatatc agttcaattc tttcaaggaa tacggaaata
      601 gctgcggcag gtgtaagtgc cgatatggat tatatagaaa atgaaacgga tctggttctt
      661 gatgaagacg tgtcgatctc tttagatgat tttgacgcac tttgtaagat cgtttattct
      721 gaagcaggct gtgaagacat ggaaggtcag atcatgatcg cgaatgttgt attgaacagg
      781 acaaaatcag atatttttcc ggatgatatc cagaaagtta ttatgcagcc cgggcagttt
      841 gagccggtga cctgcggttc ttatttttac gcggatccgt cgaagatgac aagggaagct
      901 gttatgagtg cattgaatgg ggaagatttg tcaaacgggg ctctttattt tcaaaaaagc
      961 agttctaaag tatgggatga tcatgtatat ttgttcagac atggaagtca ttcgttttat
     1021 aagtaaaccg aataggaaag cggcagtcct ggatttttgg ggactgcctt ttacgataca
     1081 ggatcgtttt ttttatttaa acgcatgnnn nnnnnnnaac ccggatacgt atataaagag
     1141 aagcatattg ggacggagcc attgtgtcaa tgtaattctg gttatagcgt ctttcgggaa
     1201 attttttttt gaccagatcg accgctttgt tgtaggcaac ggcggcagct gtttccgtat
     1261 cgtatgttcc gactgtgatc ataccgttta tgtgaatttg tgctttgtat ctggttttgt
     1321 ttttttgaca aatttttgtt acgccgtaaa attggttgat gatctcaatg ttttcatatc
     1381 tgaaatcaaa gcagtctccg ttaataaacc gataaccccg gtccattacc gcatggcttc
     1441 tgatcccata tcttgagggc agactgatct gcattccgta gtcggcaaca aacagatgtc
     1501 cgccccgttt tgatatttta tgccctgcat aatagaacag atcgtcaata tcaaatttgt
     1561 agacaagttc ttctgtcaga taatagagaa aataatgttt ttcaagatag attggatttt
     1621 tgaaataaac attgttatca cggaaattaa caatggacac atatttatca aaggacagaa
     1681 tatgtgtatc tgcctgatag gaaagaatgg atgctgaatc ggaaataagc cggctggcat
     1741 ccagataggc ctgatgtgcc agttcttccg actcaaagct tccaagactg atatgtttgt
     1801 ttttgtaggt aaacgaacta cggtaataaa tcgttccgtt ttttgctgtc gctatatatg
     1861 cgcctgctaa tgtcatgaat tcattcgctc cgctgcgtga ttttttatac gattttctgc
     1921 ttttaaaatc agtgtacagg aattcataca ggaattcaat aatgaattcc tgtatgaaca
     1981 gtgctatact gtaactgaaa tcgtctggca ggaggaatta ttatggcaag atcttcaaat
     2041 caaaaactga agctgctgta tttgttgaaa atactgactg aacatacaaa cgagcagcat
     2101 ccgatgaata cggcagccct gataaaagcg ctggaggcat atggaatcag tgctgagcgc
     2161 aaaagtatat atagtgatat cgaagaactg atccattttg gtattgatat tgagaaatcc
     2221 ggaggcagga gaaatgccgg ttattatgtg gcaggcaggg aatttgaatt ggcggagctg
     2281 aaacttcttg tggatgcggt ccagtcttca aagtttatta cagggaagaa aacagacagc
     2341 ctgatcagga aactgggact tctgactgat aaatatcaag ctgcaaagct gaaaagacag
     2401 gtttttgtgg caaacaggac gaaaacacag aatgaaagta ttttttataa tattgacgat
     2461 atttacaggg caattcagga cggaaaagag atcagttttc agtattttga gtggagtatc
     2521 aataaggaac tgctgctgaa aaaaaacgga gaagcatata ttgccgggcc gcttgcgctg
     2581 atctgggacg acgaaaacta ctatcttgtt gcatatgata aaaaagcagg attcaggaaa
     2641 catttcagag ttgataaaat gaaaaatatt caggttctgg aaagcaggcc ggatattatg
     2701 gaccgatcgt ttgatattgc agcatactca aaaaaaatgt tcggcatgtt cggcggggag
     2761 acagacgatg tgatccttag atgtccaaac gggaaaatcg gtattctgct tgatcgcttc
     2821 ggaaaggaaa ttacagtaag gcagctggac ggagatttct gcagagtgcg tgtacaggtc
     2881 gttgtcagcg aacagttttt cggatggtta tgtgcgatcg gaaccgatat ttcgattgtg
     2941 gcacccgcac agataaaaga acaatacagg atgcatctga aaaagatttt ggacaattat
     3001 gagtagtttt tttgtgtact atttctattg acatcataga ggcaacttgc tatattttat
     3061 ttgggtatac tatatgttga tgccgatttg gcgtcaatac taaatgtaga tagagagcgg
     3121 gaggatatca tatgggttcg gaaacggctg tttcagaggg aaaaaagaat tattcgcaga
     3181 tgtataaacc ggacagggac gtcaggatgg tcaagaagga cggtacactg gaggcattta
     3241 atgtacaaaa ggtcattgat gctgtcggaa aatctgcata tcgcgcattg acaaaattta
     3301 ctgaccagga aaaggaatat atatgtcaga aagtgattga cagagtaaac gaattgggtg
     3361 ctgacgaaat tccaattccc atcatgcata atatagtgga aagcgcattg gaggaagtaa
     3421 agccgatcgt ggcaaaaagc tatagggatt atcgtaatta taagcaggat ttcgtgcgca
     3481 tgcttgacga tgtttacaaa aagagtcagt ccattatgta tattggtgac aaagagaatt
     3541 caaatacgga cagtgcgctt gtttctacga aaagaagtct gatatttaat cagctgaata
     3601 aggagctgta tcagaaattt tttatgacga ctgaagaaat acaggcatgc agggacggtt
     3661 atatttatgt tcatgatatg tccgcaagac gtgatacgat gaattgctgt ctgtttgatg
     3721 tacagtctgt tctgacgggc ggttttgaga tgggaaatat ctggtataat gaaccgaaaa
     3781 cactggatgt tgcttttgac gtgataggag atattgtatt atcggctgcg agccagcaat
     3841 acggcggctt cacggtaccg agcgtggatt atatattaga gccttatgcg gaaaaatctt
     3901 atcggaaata tctggacaaa tatgagcgcc ttggtattga cagggaaaaa gccgaagcag
     3961 aggcgtatgc agatgttaca agagaatttg aacagggctt ccagggatgg gaatataagt
     4021 tcaatacagt tgcgtcaagc agaggtgatt atccgtttat tacggtaacg gccggtacgg
     4081 gaacgggccg gttccaaaag
//
LOCUS       DAOSYE010000066         4083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15148_length_4083_cov_3.535253, whole genome shotgun sequence.
ACCESSION   DAOSYE010000066 DAOSYE010000000
VERSION     DAOSYE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15148_length_4083_cov_3.535253"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /gene="yicI"
                     /locus_tag="PLU43_01895"
     CDS             <1..1509
                     /gene="yicI"
                     /locus_tag="PLU43_01895"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HOO27190.1"
                     /translation="GRPALPPAWSFGLWLSTSFTTNYDEATTSSFVQGMFERDIPLSV
                     FHFDCFWMKEFHWCDFEWDERVFPDVKGMLQRYKEKGLKIDVWINPYIAQGTDFFKEG
                     AENGYLLMRADGRGVKQIDTWQAGMGLVDFTNPAAVKWYTDKLKTLLDAGVDCFKTDF
                     GERIPVDVAYYDGSDPVTMHNYYTYLYNQAVFDLLKREKGEGEAVLFARSATAGCQQF
                     PVHWGGDCSASYPSMAETLRGGLSFAMSGFSFWSHDISGFEQTAPPDLYKRWVAFGLL
                     STHSRLHGSTSYRVPWLFDEESSDVVRFFTKLKCRLMPYIFSKAVEAHEKGTPVMRPM
                     IFEYMSDPAANYTDMQYMLGESLLVAPIFNDKGTADYYLPAGRWTHLLTGEKRDGGRY
                     YHDSYDYFSLPLYVRENTILAMGAVDTRPDYAYENGVTLHIYELGDGERGCCDIVSME
                     GTHVMQAEAVRVDDTITLKLTASPENMKVCIHADKTEKKEIPVNSMETIIRL"
     gene            1618..2340
                     /locus_tag="PLU43_01900"
     CDS             1618..2340
                     /locus_tag="PLU43_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOO27191.1"
                     /translation="MKSKGLKALVVTGIIILTAFLVYQAFGEMLPQLFKVLKSGNEKE
                     IADYLENVSTWRGLLCTVLLQFIQVISIVLPGMPIQIAAGMVYGVWRAFVLTYFGYVS
                     ANVTVFLVARHVGSEIVDKLPVKAKDNRLMKRINTVRPEFVVAMACLMPGVPNGIVPY
                     IAANTKISVRNFMAAVAAGSGVPILLSCLTGHFLLTGDYVFSVLSIVILWILIITAFK
                     KQDWILWKLDKIKNNQTKRNAH"
     gene            2502..3554
                     /locus_tag="PLU43_01905"
     CDS             2502..3554
                     /locus_tag="PLU43_01905"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOO27192.1"
                     /translation="MKTRLVRINEEEIDEKAIRCAGHIIKAGGLVAFPTETVYGLGAD
                     ALNKEASEKIYAAKGRPSDNPLIVHIAELSDLCKIADNIPPAAWALADTYWPGPLTMI
                     FQKKDIVPKETTGGLDTVAVRMPGHKVALAFIREAGGFIAAPSANISGRPSPTLAKHV
                     EQDLNGRIEMIIDGGAVNIGLESTIVDMTSEIPTILRPGYISKEMMEEVLGEVAYDQA
                     IFKSVDLKTPPKAPGMKYKHYAPKGELVIVKGAQTEVTEFIRREIKNRQAGGEIVGVI
                     AATETCSLYEAPVVENIGTREDEETIARHLYAVLREFDEKGVTYIFSEEFNTPLMGQA
                     IMNRLMKAAGHRVIKV"
     gene            3620..4054
                     /gene="rpiB"
                     /locus_tag="PLU43_01910"
     CDS             3620..4054
                     /gene="rpiB"
                     /locus_tag="PLU43_01910"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOO27193.1"
                     /translation="MKIAIGSDHGGFDLKETVRAHLGEKGFEVQDFGCYDRNSCDYPE
                     YGRAVAKAVASGECDKGVVICTTGIGISITANKVPGIRAALCGDCASARLTREHNDAN
                     ILAMGAGIIGQNLALDIVDIFFGTEFSGVEKHARRIGKIEED"
BASE COUNT         1138 a          752 c         1146 g         1047 t
ORIGIN      
        1 ggcagaccgg cgcttccgcc ggcatggtcg ttcggtctct ggctctccac ctcgtttacg
       61 accaattatg atgaagcaac gaccagttcg tttgtccagg gtatgtttga acgggacatt
      121 ccgctgagtg ttttccattt tgactgcttt tggatgaaag aattccactg gtgcgatttt
      181 gaatgggatg agcgggtatt tccggatgta aagggaatgc tgcagcgcta taaggaaaag
      241 ggtctgaaaa ttgatgtatg gatcaatcca tatattgcac agggaacgga cttttttaaa
      301 gaaggtgcag agaatgggta tctgctgatg cgtgccgacg gcagaggtgt caagcagatc
      361 gatacatggc aggcaggtat gggtcttgtg gattttacaa atcctgcggc tgtgaaatgg
      421 tatacggata aactgaagac acttcttgat gcgggtgtgg actgctttaa aacggatttc
      481 ggagagagga taccggtgga tgttgcttat tatgatggtt ccgatcccgt aacgatgcat
      541 aattattata cgtatttata taatcaggcg gtatttgacc tgctgaaacg agaaaaggga
      601 gaaggtgaag cggtgctgtt tgcaagaagc gcgacagcag gctgtcagca gttccctgtt
      661 cactggggcg gcgactgctc cgccagctat ccttccatgg cagagacttt acgcggaggt
      721 ctgtcgtttg ccatgtcagg attttcgttc tggagccatg atatttccgg ttttgagcag
      781 accgcaccgc cggatctgta taaacgctgg gtcgctttcg gacttctgtc cacacacagc
      841 aggctgcacg gttccacaag ctatcgtgtg ccgtggctgt tcgatgagga atcaagtgat
      901 gttgtccggt tttttacaaa gctgaaatgc agactgatgc cctatatttt cagtaaagcc
      961 gtagaggctc atgaaaaggg aacgccggtc atgcggccga tgatatttga atatatgtcg
     1021 gatcctgcgg caaattatac ggatatgcag tatatgctcg gagaaagctt gcttgttgct
     1081 cctattttta acgataaagg aacggcggac tattatctgc ctgccggaag atggacacat
     1141 ctgcttacag gggaaaagag agacggcggc cggtattatc atgacagcta tgattatttt
     1201 tcgcttccgc tgtatgtgcg tgaaaatacg atactggcta tgggggcagt cgatacaagg
     1261 ccggattatg cttatgaaaa tggcgtgaca ctgcatattt atgagctcgg tgacggagaa
     1321 cgcgggtgct gcgatattgt cagcatggaa ggaacgcatg tgatgcaggc agaagcggta
     1381 agggtcgatg atacgatcac cctgaagctg acggcatcgc ctgagaacat gaaagtctgc
     1441 attcatgcgg acaaaacaga aaagaaagaa atcccggtaa atagcatgga aacgatcatt
     1501 cggctgtagt gtacggcgat acaaatatat tcggcagata aaacggtttc ggttgaaaaa
     1561 gaagtaaaat aatgatagag tgataggtag tgtagataca gagaaaagga gaaaccggtg
     1621 aagtcgaagg gattaaaggc gcttgttgta acagggatta ttattctaac agcatttttg
     1681 gtctatcagg cattcggtga aatgctgccg cagctattta aggtattgaa atccggaaac
     1741 gaaaaagaaa ttgccgatta tctggaaaat gtcagcacat ggcgggggtt gctttgtacg
     1801 gtgcttttac agttcattca ggtgatttcc atcgttttgc ccggaatgcc gatccagatc
     1861 gcagccggta tggtttatgg agtatggagg gcatttgtcc tgacttattt tggttatgtt
     1921 tcggcaaatg tgacggtttt ccttgtggcc aggcatgttg gttccgaaat agtcgataag
     1981 cttccggtaa aagcaaaaga caaccgtctg atgaagcgga tcaatacggt ccggccggaa
     2041 tttgttgtgg cgatggcatg cctgatgcca ggtgtgccga atgggatcgt accgtatatt
     2101 gctgccaata cgaaaatttc cgtcaggaat tttatggcgg cggtcgcggc gggaagcgga
     2161 gtgccgattc ttttgtcctg tctgacagga cattttctgc tgaccggcga ttatgtgttc
     2221 agtgttttat caattgtgat tttatggatt cttattataa cggcatttaa aaaacaggac
     2281 tggatattat ggaagctcga taaaataaag aataaccaga ctaaaagaaa tgcgcattga
     2341 acaaccaaca gtttacagga aactgttggt tgttttttgg agcctctttt ataatgaaat
     2401 ttcaaaaatg aaacggtatt gcagtttgca ttgatgataa tctgtgctat actattcaca
     2461 gtaggcattt ttgccttcaa ggaataaaaa gaaggatttt catgaaaaca agattggtca
     2521 gaataaacga agaagagatt gacgaaaaag cgatcagatg tgccggccac attattaaag
     2581 cgggcggttt ggtcgctttt cctacggaaa cagtttatgg acttggcgca gacgcgctaa
     2641 ataaagaagc atccgaaaaa atttatgcag caaagggaag accctctgac aaccctttga
     2701 ttgttcatat tgccgagttg tcggatttat gtaaaatagc tgataacatt ccgcctgcgg
     2761 catgggcact cgccgatacc tattggccgg gcccgcttac gatgatcttt caaaaaaagg
     2821 atattgtacc aaaggaaact acgggcggac ttgacacggt tgccgtcaga atgccgggtc
     2881 acaaggtagc gcttgctttt atcagggaag ccggtgggtt tattgccgca ccgagtgcaa
     2941 atatttcggg ccgtccgagt ccaacacttg caaaacatgt ggaacaggat ctgaacggca
     3001 ggatcgagat gatcattgac ggcggagcgg taaatatcgg tctggaatcc acgatcgtgg
     3061 acatgacgtc ggaaatcccg acgattctgc gtccgggata tatttctaaa gaaatgatgg
     3121 aggaagtgct tggagaggtt gcgtatgatc aggcaatttt taaatcggtc gatcttaaaa
     3181 cgccgccgaa ggcacccggc atgaaatata agcactatgc accaaaagga gaactggtca
     3241 tcgtcaaggg tgcacagact gaggtgacgg agttcattcg aagagagatc aaaaacaggc
     3301 aggcaggggg agaaattgtc ggcgtgatcg cagcgacaga gacctgttcg ttatatgagg
     3361 cgccggtagt tgaaaatatc ggaacccgtg aggatgaaga aacgatcgcc agacatttat
     3421 atgcggtttt acgtgagttt gatgagaagg gtgtgactta tattttttcc gaagagttta
     3481 acaccccgct tatgggacag gcgatcatga accggctgat gaaggcggcg ggacatcgcg
     3541 tcattaaggt gtaaagcagg taatggtccg gtatgcaaaa ggatggcggc agccggatga
     3601 cagataaagg aggaaaaaca tgaagatagc aattggcagt gatcacggtg gatttgatct
     3661 aaaggagacc gtaagggccc atttggggga aaaaggtttt gaggttcagg attttggctg
     3721 ttatgacagg aattcgtgtg attatccgga atacggaaga gcggtcgcaa aagcggtggc
     3781 atccggggaa tgtgataagg gagtcgttat ctgtacgaca gggatcggta tttccattac
     3841 ggcaaacaag gttccgggta ttcgtgcggc gttgtgcggc gactgtgcta gtgcgagact
     3901 gacaagagag cataacgatg cgaatatatt ggccatgggt gccggtatta tcggacaaaa
     3961 tcttgcgctt gatattgtgg acattttttt cggtacggag ttttccggtg ttgaaaaaca
     4021 tgccagaagg atcggtaaaa tagaagaaga ctgataaaat aaaacactca ggaggaagaa
     4081 aaa
//
LOCUS       DAOSYE010000067         4066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15244_length_4066_cov_3.298679, whole genome shotgun sequence.
ACCESSION   DAOSYE010000067 DAOSYE010000000
VERSION     DAOSYE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15244_length_4066_cov_3.298679"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2696)
                     /locus_tag="PLU43_01915"
     CDS             complement(<1..2696)
                     /locus_tag="PLU43_01915"
                     /inference="COORDINATES: protein motif:HMM:NF040034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6240 domain-containing protein"
                     /protein_id="HOO27194.1"
                     /translation="MKIELGVQDINQNVDKEKTAYSNPSQKDTARTAETTSGYSLDIS
                     GTVTDNAAYGTGDLKSAKDIMQDAGQKDIALQRDYMAVMSNSMSTEDFAELEKDGYSP
                     LNTEIETQVTVVDKIKATLAEAGVVVTGYNDDLSSEQLTEITGSTGRADQIEKTLREN
                     DLPVTEDNIRQMDAVLTQAENIRPLSDDAVKYMLNNKLEPTIGNLYKAEYSAGNDSGK
                     QARGFYQDDTAGYYAKKADTVEWEQIEKQVESIVEKAGLPADEQTMDNARWAVEKGVL
                     LTQDTLVRMDEIKSISIPLEETQLLSAMATAITDGRSPEQASLVQTESIAKQAVSLVN
                     DVAAISDEAVRQVVTNDDELTIRNLKEYDRQIGEAQSEETGSLTEESNSDSLVSAKLF
                     LEETRLQMTVEANIRLLKKGISVDTTSLEKLVEELKTAEQEYYQPLLGNESDDQSETE
                     NSTLSGKISLYKETDSLLSQIGSVPAAVLGKLTAENRTLTLSTVYEEGSTLKSTYEKA
                     GQTYEALMTAPRADMGDSIEKAFQNTDELLDEMDLETTEANRRAVRILGYSNTQIDRD
                     SVETVRLADQAVTNVIEKMTPAKTLQMIRDGENPINTDIYELSEQLDQKNEDEEAEKY
                     SEFLWRMEKNDEITADERSAFIGMYRLFNQIEKSDGELIGNVLSSNEPLTLKNLLSAS
                     RSNRATGMDVSIDDDFGGLEELIQSGESISEQITAGFGTGEHRSQSYYEGLARDVFDS
                     LTPEGLQNTEITADITLESFADELSQNAGNESYAMEYLNEQLSDIKTAAQVEDAVVKA
                     LQDYDEPVTINQLLAADMLMNQRGNAYEKLMNQSKEKSIRKEKLENAISQLQEGFNSE
                     EEAAAAYEEMTDAAKEIVSDELYLADNSVDVKEMNLLFKQLSV"
     gene            complement(2871..>4066)
                     /locus_tag="PLU43_01920"
     CDS             complement(2871..>4066)
                     /locus_tag="PLU43_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27195.1"
                     /translation="IGIGFIALSLRIPPKDDMSAETKHTGFKSGALIVSTYLLQGILG
                     LLISISLAFTVLPDLFKASGVLLPMGYGQGPGQANNIGSTYEQLGFKGGQSFGLSISA
                     AGFLCACIIGVIYINYLVRHKKLKRQEAQFVSGSITVGDFQDKNEIPVSESIDKLSVQ
                     IAFVLLLYLVTYFAAFGLTMLLRAVAPGLAKNLTSIIWGFNFIIGSILAIGCRAALHG
                     LKKVKLMTRQYQNNYLLSRISGMAFDFMTVAGISAIDFQELKGLWIPFILMTVLGGLV
                     TFLYCRWMCRRIYPGYEEEGLISMFGMLTGTISSGVLLLREVDSGFRTPAANNLLTGS
                     SFAIVLGIPMLVLIGMAPESDTKLFITLGLMLIYFVFLLFLIRKLSDHKKNKNFLEKD
                     EELSK"
BASE COUNT         1001 a         1052 c          752 g         1261 t
ORIGIN      
        1 acggaaagct gcttaaataa taaattcatt tctttcacgt ccacagaatt gtccgccaga
       61 taaagttcat ctgaaacgat ttcttttgca gcatctgtca tctcttcata ggcagcggcg
      121 gcttcctcct ccgaattaaa tccttcctgc agttggctga tcgcgttttc cagtttttct
      181 ttccggatgc tcttctcctt tgactgattc atcagcttct cataggcatt tccgcgctgg
      241 ttcatcagca tatccgcagc aagcagctga ttgattgtga ccggctcgtc ataatcctgc
      301 agtgccttta ccacagcatc ttcgacttgt gccgccgttt ttatatcaga aagctgctca
      361 ttcaaatatt ccatcgcata gctttcattt cctgcattct gactcagctc atcagcaaac
      421 gattccagcg ttatatctgc cgtgatttcc gtattctgca gaccttccgg cgtcaggctg
      481 tcaaaaacat cccttgccag tccttcataa taagactgtg atcgatgttc gcctgttcca
      541 aaacctgcgg taatctgttc cgaaatagat tctccgctct gtatcagctc ttccagtcct
      601 ccaaaatcat catcaattga aacatccatt cctgttgccc tgtttgaacg cgatgcggac
      661 aatagatttt tcagtgtaag cggctcgttg ctgcttaata catttccgat cagctctccg
      721 tcactttttt ctatttgatt gaaaagccgg tacattccga taaaagcact tctttcatcg
      781 gcagtgatct catcattttt ttccatccgc cacaaaaact cactgtattt ttctgcctct
      841 tcatcctcat ttttttgatc aagctgctct gataattcat aaatatcggt attgatcgga
      901 ttttcaccgt cacgtatcat ttgcagcgtc ttcgcgggcg tcatcttttc aataacattt
      961 gtgactgcct gatcggcaag tcgtactgtt tcaacagaat ccctgtcgat ctgcgtattg
     1021 ctgtaaccaa ggatccggac tgcccttcga ttcgcttctg ttgtttcaag atccatttca
     1081 tcaagcagtt catccgtatt ctgaaatgcc ttttctatag agtcgcccat gtccgctctc
     1141 ggcgctgtca tcaatgcctc atacgtttgt cctgcttttt cataagtact ctttagtgtg
     1201 gatccttctt catataccgt tgaaagtgtc agcgtcctgt tctctgccgt aagctttcca
     1261 agcacggctg caggaactga tccgatctgc gacagcaggc tgtctgtttc tttatataga
     1321 ctgatttttc cggaaagcgt gctgttttcg gtctcacttt gatcatcgct ttcatttccc
     1381 agaagcggct gataatattc ctgctcagct gtttttaatt cttcaacgag cttttccaaa
     1441 gatgtcgtat ctacggaaat tccctttttc aaaagcctga tattcgcttc aaccgtcatc
     1501 tgaagcctgg tttcttccaa aaaaagtttt gcagacacca gtgaatcact gtttgactct
     1561 tcggttaaag atcccgtttc ttccgactgc gcttccccga tctgcctgtc atattctttt
     1621 aaattccgga tcgtcagttc atcatcattt gttacaacct gccggactgc ctcatcagaa
     1681 atcgccgcca catcatttac aagggaaacg gcctgcttag caattgattc agtctgcaca
     1741 agagatgcct gttccggcga tctgccgtcg gttatggctg tggccattgc gctcagcagc
     1801 tgtgtttctt ctaatggtat cgagatactt tttatttcat ccattcgcac taacgtatcc
     1861 tgcgtcagta aaacaccctt ttcgactgcc catctggcgt tgtccattgt ttgttcatcc
     1921 gcaggaagac ccgccttttc aacgatcgac tcgacctgct tttcaatctg ttcccactcg
     1981 accgtatctg cctttttcgc ataataacct gccgtatcgt cctgataaaa acctcttgcc
     2041 tgttttcctg aatcgtttcc ggcactgtac tctgccttat atagattccc gatcgtcggc
     2101 tccagtttat tgttgagcat atatttgact gcgtcgtccg acaatggccg gatattttct
     2161 gcctgcgtca gcactgcatc catctgccgg atattgtctt cagtaacagg aagatcattt
     2221 tctctcagcg ttttttctat ctggtctgct ctgcccgtac tccctgtgat ttcagtcagc
     2281 tgttcactgg aaaggtcatc attatagcca gttacaacta caccggcttc cgcaagcgtt
     2341 gccttgatct tatcaacaac tgtcacctgt gtttctattt ccgtattaag cggtgaatac
     2401 ccgtcttttt caagttccgc aaaatcttct gttgacatgg aattagacat aactgccata
     2461 taatctctct gtaaggcaat gtccttttgc cctgcatcct gcataatgtc ctttgccgat
     2521 tttaaatccc cggttccata tgcagcgtta tccgtaactg tgccagaaat gtccaatgaa
     2581 tatcccgatg tcgtttctgc tgtccttgca gtatcttttt gactcgggtt actgtatgca
     2641 gttttttctt tgtcaacatt ttgatttata tcctgtacgc cgagttctat tttcatatgc
     2701 ttcttcctcg atcaaaatgt atcgtcttcc ttgaatgaat ttgaattatc tgtactatat
     2761 ttcggcagtc taaacgaatt tctttaccct aaaataattg gaaacagaac aacccgccga
     2821 taaacagctg cctgcttccg gcgggtttcc ttttaaacag atcgattcag ctatttggaa
     2881 agctcttcgt ccttttccag gaaattttta tttttcttat gatccgataa ctttcgtata
     2941 agaaacagca ggaacacaaa atagatcagc atcagaccaa gcgtaataaa cagctttgtg
     3001 tctgattccg gtgccatccc gatcaagaca agcattggaa ttccaagtac gatcgcaaaa
     3061 ctgctgccgg tcagcagatt attggcggca ggcgtacgaa acccggaatc gacttcccgc
     3121 agtaaaagga cgcccgaact gatcgttccg gtcagcatcc cgaacatgga aatcagacct
     3181 tcttcttcat agccgggata aatacgtctg cacatccatc tgcagtataa aaaggtaacg
     3241 agacctccaa gtactgtcat taaaataaat gggatccaaa gtcctttcag ttcctgaaaa
     3301 tcaattgcgg aaatacccgc aactgtcata aaatcaaatg ccattccgga aatccggctc
     3361 agcagataat tattctgata ctggcgtgtc attaacttta ctttctttaa tccatgcaga
     3421 gccgccctgc agccgatcgc aagaatcgaa ccgataataa agttaaagcc ccatataata
     3481 gatgtcagat tttttgcaag tcccggagcc accgcgcgaa gcagcatcgt cagaccaaac
     3541 gcggcaaaat atgttactaa ataaagcaga aggacgaacg ctatctgaac ggacagttta
     3601 tctattgact ctgagaccgg aatctcattt ttatcctgaa aatcccctac ggtaatagaa
     3661 ccggaaacaa actgagcctc ctgccgtttt aactttttat gccgtacaag ataattaata
     3721 taaatgactc cgataataca ggcacacaaa aatccggcgg cggaaataga aagtccgaag
     3781 gactgccctc ccttgaatcc aagctgctca tacgttgatc caatattgtt cgcctgaccg
     3841 ggcccctgcc catatcccat cggaagcagg acgccggaag ccttaaacag atcgggaagc
     3901 actgtaaaag ccaaacttat tgaaatcagc agacctaaaa ttccctgaag cagataagta
     3961 ctgacgatca gcgctccgct tttaaatccg gtatgttttg tttctgccga catatcatct
     4021 ttaggcggta tccgcaggga aagcgcaata aatccaattc cgatac
//
LOCUS       DAOSYE010000068         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15254_length_4064_cov_3.423298, whole genome shotgun sequence.
ACCESSION   DAOSYE010000068 DAOSYE010000000
VERSION     DAOSYE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15254_length_4064_cov_3.423298"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLU43_01925"
     CDS             <1..823
                     /locus_tag="PLU43_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO27196.1"
                     /translation="KSFNELAEVTFGLDFENWYKNGYWTDRYVPYSIITGNKVVSNVS
                     VNKMEFAVNGENKKYMQLGTVMTDEKYRNQGLIKRIMQEIEKDFQDSVDGFYLFANDR
                     VLDFYPRFGYRKGIEYQYSKVLRSHLHRQAKQISMNHKEDWLILEKAIELSVSNSAFE
                     MRNNKELIMFYVTQFMQENVYYIEEEGAYVIAEINGNELFLHNIFAEKEVNMETIIDA
                     FGDGLDQVVFGFTPLTSQGYQVKRLCEKDTTLFLKGRDFDLFEQKRGMFPSLSHA"
     gene            863..1537
                     /locus_tag="PLU43_01930"
     CDS             863..1537
                     /locus_tag="PLU43_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO27197.1"
                     /translation="MEKNIVYEAKLYMEQLTKDAAHDREHIYRVVNMALDIAKTEMLI
                     DMEILLTACILHDIGREKQFENPKLCHAREGAKMAYKFLREKGVSPEEAIKVKACIET
                     HRFRSERQPESIEAKILFDADTLDVTGALGIARSLLYEGRMGIPLYHVDSRGSIIDGK
                     DDYQDSFLKEYDYKLFNVYNRLYTKRGKEIAQERKKLTTAFYEELYREISFGRGNMEA
                     MLEIDK"
     assembly_gap    1733..1832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2312..3409
                     /locus_tag="PLU43_01935"
     CDS             2312..3409
                     /locus_tag="PLU43_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOO27198.1"
                     /translation="MIFGGLESLPNIKKGKSRAINAENPYGEKGKGGMTSSVLGPSRK
                     GSPCIEQLESGSITTLGEIGGAGVIQHIWVTVTDRTEEDFYVLRDLVLRIYWDEEEAP
                     SVETPLGDFFCCGFGRGCIVNSMPIAVNPARGMNCYFPMPFNKKAKITIENQHSCAIP
                     AFFYQIDYLLLDEPQTDVAYFHARWNREKLTKTKRDYTILDQVKGKGHYVGTYLALTT
                     LERYWWGEGEVKMYLDGDEEYPTICGTGTEDYFGGAWSFASQKDGKTVENTYCTPYLG
                     YPYYSSHDDSVHNFYHNDDIMPQRGFYRWHIADPVLFEEDIRVTIQQIGVCHKGLFER
                     QDDVASVAYWYQTEPHVPFQPLLKKEDRWPR"
     gene            complement(3485..>4064)
                     /locus_tag="PLU43_01940"
     CDS             complement(3485..>4064)
                     /locus_tag="PLU43_01940"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO27199.1"
                     /translation="MYQTKLLESKSMRGNTINALMTTLDEKSEETKAHTLRLSDICLK
                     IAEQLEQHTEIKNRLALLAVLHDIGKVGIPDHILGKPEALTEDEWQIMKTHPEIGYRI
                     ASNVPELHAVANEILHHHEKWDGTGYPSGLKGEKIPINCRILSVVDSYDAMTNDRVYR
                     KARTHEQAMDELRRFAGIQFDPIIVAAFAAIY"
BASE COUNT         1242 a          668 c          994 g         1060 t
ORIGIN      
        1 aaaaagcttt aatgaactgg cagaggtgac gtttggcctt gattttgaaa attggtataa
       61 aaatggatat tggacagatc gctatgtccc ctattcaata atcacaggga acaaagtagt
      121 atccaatgtt tccgtaaata aaatggaatt tgcggtcaat ggagaaaata aaaaatatat
      181 gcagcttggc actgttatga cagatgaaaa ataccgtaat cagggactga tcaagaggat
      241 catgcaggaa attgaaaaag attttcagga cagcgtcgat ggcttttatc tttttgccaa
      301 tgatcgtgtg ctggattttt atcctcgatt cggatataga aaagggatag aatatcagta
      361 ttccaaagtt ttacgcagtc acttgcacag acaggcaaaa cagatttcaa tgaatcataa
      421 agaagactgg ctgatattag aaaaggcaat agagctttct gtaagcaaca gtgcatttga
      481 aatgagaaat aacaaggaat tgatcatgtt ttacgtcaca caattcatgc aggaaaatgt
      541 ttattatatt gaagaggagg gggcttatgt cattgccgag atcaatggaa acgaactgtt
      601 tttacataat atttttgcag aaaaggaagt aaatatggaa acaattattg atgcgttcgg
      661 agatgggctc gatcaggtgg tatttggttt tacgccgctg acaagtcagg gatatcaggt
      721 taaacggctg tgcgagaaag atacaacact gtttttaaaa ggcagagact ttgatctgtt
      781 tgagcagaag agggggatgt ttccaagcct ttcgcatgcg tagcacaaga aaagaatgtt
      841 ttcagaaagg gtaagaaagc atatggagaa aaacattgtt tatgaagcaa aattatatat
      901 ggagcagctg acaaaagatg ctgcacatga tagggaacat atttacagag tggtgaatat
      961 ggcattagat attgcgaaaa cagaaatgct gatagatatg gaaatattgc ttactgcgtg
     1021 catcttgcat gatattggaa gagaaaagca atttgaaaac cccaaattat gccacgccag
     1081 ggaaggggct aaaatggcct acaaattttt acgtgaaaaa ggggtaagcc ctgaagaagc
     1141 aataaaggtc aaggcgtgca tagaaacaca tagattcagg agtgaaagac aaccagaaag
     1201 tatagaagca aaaatattgt ttgatgcgga tactttggat gtcacagggg cgcttggaat
     1261 tgcaagaagc ttactgtatg agggcaggat gggaatacca ttatatcatg tggacagcag
     1321 gggcagcatt atagatggaa aagacgatta ccaggattct tttttaaagg agtatgatta
     1381 taagttattc aatgtttata acagactcta tacgaaacgg ggaaaagaaa tagcacaaga
     1441 gcgaaaaaaa ttaacgacag ccttttatga agagctttac agggagattt cttttggccg
     1501 aggaaacatg gaagcaatgc tggaaataga caaatagata tatagataga aagaagttta
     1561 ctgttgtaaa tgatacaaag gtattagacc taagatatga aaagaataaa ttgcgtttag
     1621 ccataattac ttgtcgtttc atatcatttt ttagatatca atagcataat tttatgttgt
     1681 tatttattca gtcgttccct acgcgggaac gtggattgaa attggcttag gannnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaccagtg tgcggccgga ttacagtcgt
     1861 tccctacgcg ggaacgtgga ttgaaattgg cttaggacac cagtgtgcgg ccggattaca
     1921 gtcgttccct acgcgggaac gtggattgaa atacagatac tatgcgcgag gctcccctct
     1981 ttcgcaggtc gttccctacg cgggaacgtg aattgaaatt tttcgggaga aggcgattct
     2041 gccgttaggt ctgtcgttcc ctacgcggga acgtggattg aaatgctaaa acagatttga
     2101 gagatatacc gtctgccgtc gttctctacg cgaaaacgtg ggttgaaata tcctttttta
     2161 attcaaaaaa tgcaggaata gtatagatga aaaataataa tttattggct atgcggataa
     2221 taaccttttc gctataatag agttattcaa gattactaga atagtaagcg gaaacaaaaa
     2281 cagcacaagt gttctgaaag gagaaacaaa catgattttt ggaggattag aatcattacc
     2341 aaatataaaa aagggaaaaa gccgggcgat caatgcggaa aatccatacg gagaaaaagg
     2401 aaaaggcggg atgacttcca gtgttcttgg gccatcaaga aaaggcagtc cgtgtattga
     2461 acagctggag tcgggaagta tcacgacact tggtgagatc ggcggggcgg gtgttattca
     2521 gcatatctgg gtgacagtga cagacagaac agaagaagat ttttatgttc tgcgggatct
     2581 ggtgcttagg atctactggg atgaggaaga agcgccttcg gtcgagacgc cgctgggaga
     2641 ctttttttgc tgcggttttg gaagaggatg tattgtaaac tccatgccga tagcagtgaa
     2701 cccggccagg ggaatgaatt gttattttcc aatgccgttt aataaaaagg caaagatcac
     2761 gatagaaaat cagcattcat gcgcgattcc ggcatttttt tatcagatcg actatctgct
     2821 gcttgatgag ccgcagacgg atgtagcata ttttcatgcg agatggaatc gggaaaagct
     2881 gaccaaaaca aaaagggatt atacgatctt ggatcaggtg aaagggaaag ggcattatgt
     2941 gggaacatac cttgcactga caacgctgga gagatactgg tggggagaag gagaagtgaa
     3001 aatgtatctt gacggggatg aggagtatcc gacgatctgc gggaccggta cggaagatta
     3061 ttttggagga gcctggagct ttgcttctca aaaggatgga aaaacagttg aaaatacgta
     3121 ctgtacgccg tatttaggat atccgtatta ctccagtcat gatgacagcg tccataattt
     3181 ttaccataat gacgatataa tgccgcagcg cggcttttat agatggcaca tagcggatcc
     3241 ggtattgttt gaagaagaca tacgggttac gatacagcag ataggcgtat gtcataaagg
     3301 attgtttgaa cggcaggatg atgtggcttc cgttgcatat tggtatcaga cagaaccgca
     3361 tgtaccgttt cagccattgt taaaaaagga agacagatgg ccaaggtgaa taaagacatg
     3421 ccgccatcaa aggcggcatg ttaaaagaat attaactaaa cggtacataa ctgctgtttt
     3481 ggattcagta aattgccgcg aaggcagcaa cgatgatcgg atcgaattga atgccggcaa
     3541 agcgtcgcaa ttcgtccatt gcctgttcgt gtgtacgggc tttgcggtag accctgtcgt
     3601 ttgtcattgc gtcataggaa tctacaacgg aaaggatcct gcagttgatc gggatctttt
     3661 ccccttttag tccggaagga taaccggtgc cgtcccattt ctcatgatgg tgcaggatct
     3721 cgttggcgac tgcatgcagt tccggaacat tggatgcgat gcggtatccg atttcaggat
     3781 gcgttttcat gatctgccat tcgtcttcag tcagagcctc tggcttccca agaatatggt
     3841 ccggaatgcc tacttttccg atatcatgca ggacggccag aagcgccaga cgatttttta
     3901 tttccgtatg ctgctccagc tgttctgcaa tttttaagca gatatcggaa agcctgagcg
     3961 tatgtgcttt tgtttcctcg cttttttcat ctaaagtggt catgagtgca ttgatcgtat
     4021 ttccccgcat acttttactt tccagcagct tggtctgata catc
//
LOCUS       DAOSYE010000069         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15350_length_4048_cov_3.832206, whole genome shotgun sequence.
ACCESSION   DAOSYE010000069 DAOSYE010000000
VERSION     DAOSYE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15350_length_4048_cov_3.832206"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLU43_01945"
     CDS             <1..836
                     /locus_tag="PLU43_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27200.1"
                     /translation="IPFLRPAEYTTDTALDFDVFFHALTFLEKEEGYIPELVVQLRPT
                     YPIRNVADIDHMIEQMLADRTIDSMRCIAPANEVPYKMWLCEEEASASSKITPLIKDV
                     KECYNMPRQQLPKVYYQNACIDVIRTEVITKHHSMSGQKIMGYKMKDNFDIDTEAEFK
                     KAEEYLKMQNGGRRFVFDIDGVVAGFRKGLDYGSAPPNRKMIDIINQLYAWGNEIILF
                     TARGYVTGIDWSETTKQQLQEWGVRYHQLLFGKPNADYYVDDKMFDMKALYELFGQSA
                     L"
     gene            847..2598
                     /locus_tag="PLU43_01950"
     CDS             847..2598
                     /locus_tag="PLU43_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOO27201.1"
                     /translation="MNRKELFQKNIATIDLVQEMIMVFRRQNFNRGDRLFPEWIRNFS
                     SLVESLFEKKKEYNTFGDIVDETRVAQMLRELMQAQEQKDYVLLADLLELKVLSFLLS
                     VQDVIRMQESGDLLPDFFEKNCSCLGKQQLVKILLDRKRVMKEDMQMPDGGMITEYTD
                     KERSCTYYIEQTSQGAPTLRLTDRAGDFYFHSNHSPLSEARLLVSNYYKPEGKEYCIY
                     GLGLGYHISALFAECRGSAPITVFESDPHIFVLALQSLDFTRFLSNGVTFVYDPDFTL
                     FMKYLQEHPGEVPLIHYPSLRNISCQSVRKRMEELFVQDSSVRNQLGEMLSNFRSNIR
                     NCSRYIDELSGSIAKKDIFLVSAGPSLDKNVRLLKEKPKSAVIVSVGTVYKKLIKEGI
                     RPDFAVFLDASDRIYGQVAGLENETVPLWIASTACGKIAARCRGETYLICQEDFHEAE
                     QYAGGHGYRTYTTGGSVSTIAFDVCIRLGAKRVIAIGLDLAYTGNKIHASNAGRRELS
                     GDVSGLLAVRASDGSTVYTTQAMEIYRQWFEKRIRQAVKETKNGEPAEFINATEGGAY
                     IAGMKYCTLQEAINNAI"
     gene            2615..3730
                     /gene="glf"
                     /locus_tag="PLU43_01955"
     CDS             2615..3730
                     /gene="glf"
                     /locus_tag="PLU43_01955"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOO27202.1"
                     /translation="MKVYDYLIVGAGLFGSIFAHEMKKAGKTCLVIDKRDHIGGNIYT
                     KEMEGIHVHMYGAHIFHTSSQTVWNYINTFAEFNRYTNSPVARYKDELYNLPFNMNTF
                     HALWGVKTPEEAKAKIEEQKAEGAGNEASEPANLEEQAIRLIGRDIYEKLIKGYTEKQ
                     WGKRATELPAFIIKRLPVRFVYDNNYFNDKYQGIPEGGYTGIIEKMLDGIEVKLGEDF
                     FADRAGYEAAADKIIFTGMIDEFYDYRFGELGYRSLRFETKLLDMDNFQGNAVVNYTE
                     YEVPYTRIIEHKHFEFGTQEKTIITKEYPADWKKGDEPYYPMNDEKNNALFKKYKALA
                     EKETRVIFGGRLGEYKYYDMHQVVARALEAASGELYD"
     gene            3723..>4048
                     /locus_tag="PLU43_01960"
     CDS             3723..>4048
                     /locus_tag="PLU43_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27203.1"
                     /translation="MIDTEGVMDDDIRVQQIEALRMADEYLKKLIPSIEEVITELDGE
                     MKEDSVDYLEQIVEGFNFMIETFNVTMDLINEERVIIDKDDINRAVLNLSDALLSNNY
                     KQAATI"
BASE COUNT         1198 a          713 c         1079 g         1058 t
ORIGIN      
        1 agattccgtt tctgcgtccg gcagaatata caacggacac tgcgcttgat tttgatgttt
       61 tttttcatgc cctgacgttt cttgaaaaag aagaagggta tattccggag cttgttgttc
      121 agctgcgccc gacgtatccg atcagaaatg ttgcagatat cgatcatatg atcgaacaga
      181 tgcttgctga ccggacgatc gattccatgc ggtgcattgc tccggcaaac gaggtccctt
      241 ataaaatgtg gctgtgtgag gaggaggctt ctgccagttc aaagatcaca ccgcttataa
      301 aagatgtaaa agaatgttat aatatgccgc gtcagcagct tccgaaggtt tactatcaga
      361 atgcctgtat tgatgttatc agaacggaag tcattacaaa gcaccattcc atgtccggtc
      421 agaagataat gggctataag atgaaggaca attttgatat cgacacggaa gcagagttta
      481 aaaaggcgga ggaatatctg aagatgcaaa acggcggcag gcgttttgta tttgatattg
      541 acggagttgt ggccggattt cgcaaaggac ttgattacgg ctcggcaccg ccgaaccgga
      601 aaatgataga tattattaac cagctgtatg catggggcaa cgaaatcata ttatttacgg
      661 cacgcggtta tgtgaccggt atagactgga gtgagacgac aaagcagcag ttgcaggaat
      721 ggggtgttcg gtatcatcag ctgcttttcg gcaaaccgaa cgcagactac tatgtggatg
      781 ataaaatgtt tgatatgaaa gcattatatg agcttttcgg acagtcggca ttatagaggg
      841 gacaggatga acaggaaaga actctttcaa aaaaatattg ccacgatcga tcttgtgcaa
      901 gagatgatca tggtgttccg caggcagaat tttaacaggg gagaccggct gtttccagag
      961 tggatacgga atttttcttc ccttgtggaa agtttgtttg aaaaaaagaa agaatacaat
     1021 acatttggcg atattgtgga tgagacccgt gtggcacaga tgcttcggga gcttatgcag
     1081 gcgcaggagc agaaggatta tgtgctgctg gcggatctgc tggaactgaa ggtgctttct
     1141 tttctgcttt ccgtgcagga cgtgatccgt atgcaggaat cgggtgatct gctgccggac
     1201 ttttttgaaa aaaactgctc ctgtctgggg aagcagcagc ttgtcaaaat actgctggac
     1261 agaaaacgtg ttatgaagga ggacatgcag atgccggatg gcggtatgat tacggaatat
     1321 accgataaag aacgcagctg tacctattat atagaacaga cttcccaggg agcgccgact
     1381 ttgaggctga ccgaccgggc aggagatttt tattttcaca gcaatcacag ccctctgtct
     1441 gaagcgcggc tgttagtgtc taattactat aagccggagg gcaaagaata ttgtatttat
     1501 ggtttaggtc ttgggtatca tatttctgca ttgtttgcgg aatgcagggg cagcgcgccg
     1561 atcactgttt ttgagagcga tccgcatata ttcgtgctgg cgctgcagtc acttgatttt
     1621 acacggtttt tgtcaaatgg tgtgacattt gtttatgatc cggattttac acttttcatg
     1681 aagtatctgc aggaacatcc cggagaggtg ccgctgatcc actatccgtc gctgcgcaat
     1741 atttcttgtc agtctgtcag aaaacggatg gaagagcttt ttgtgcagga cagctctgtc
     1801 cgcaatcagc ttggggagat gctctcgaat ttcagaagca atatcagaaa ctgcagccgt
     1861 tatattgacg aactttccgg cagtattgcc aaaaaggata tatttcttgt ctctgcgggt
     1921 ccgtcacttg ataaaaatgt ccgtctgttg aaagaaaagc cgaaaagcgc tgttatcgtg
     1981 tcggttggaa ctgtttacaa aaagcttata aaggaaggga tccgtccgga ttttgctgtc
     2041 tttctcgatg catccgacag aatttacggt caggtcgccg gactggaaaa tgaaacggta
     2101 ccgctgtgga tcgcatccac agcatgcgga aaaatagccg cacgctgcag gggagagact
     2161 tatctgatct gtcaggagga ttttcatgag gcagaacaat acgccggcgg gcatggatat
     2221 cggacatata cgacaggtgg ttcggtttca acgatcgcat ttgatgtatg tatccggctt
     2281 ggtgcaaaaa gagtgattgc cattggactt gatttggctt atactgggaa taagattcat
     2341 gcttcaaatg caggacgccg agaactgtcg ggggatgtgt ccggtctgct tgcggttcgt
     2401 gcatctgacg gaagcacagt ttatacaacg caggcaatgg agatctatcg tcaatggttt
     2461 gaaaaaagga tcaggcaggc tgtaaaagag acaaaaaacg gagagcctgc ggaatttatc
     2521 aatgcgaccg agggaggcgc ctatattgcc ggcatgaaat attgtacgct gcaggaagcg
     2581 attaataatg caatataggg aaaagggaaa ataaatgaaa gtatatgact atctgatcgt
     2641 tggagccggt ctgtttggct cgatatttgc gcatgaaatg aaaaaagcag ggaagacctg
     2701 tcttgttatt gacaaaaggg atcatatcgg cggaaatatt tatacaaagg aaatggaagg
     2761 aattcatgtt catatgtatg gggcacatat ttttcatacg agcagtcaaa cagtgtggaa
     2821 ctatataaat acatttgctg agttcaaccg ttatacgaat tcgccggtgg cgcgttataa
     2881 ggatgaactt tataatctgc ctttcaatat gaatacgttt catgcgttat ggggtgttaa
     2941 aacaccggag gaagcaaagg caaaaattga agaacagaag gcggaaggtg ccggaaatga
     3001 ggcgtctgaa cctgcaaatt tggaagaaca ggcgatccgt ctgatcggca gggatattta
     3061 tgagaaactg atcaagggtt acacggaaaa gcagtgggga aaacgtgcaa cagagctgcc
     3121 ggcatttatt attaagcggc tgccggtccg ttttgtttat gataataatt attttaacga
     3181 caagtatcag ggaattccgg aaggcggtta taccggaatc atcgaaaaaa tgctggatgg
     3241 tattgaagtg aagctgggag aggatttctt tgcggaccgg gcaggatacg aagctgctgc
     3301 ggataaaata attttcacag ggatgatcga tgagttttat gattatcggt tcggagaact
     3361 cgggtacagg agccttagat tcgaaacaaa actgctcgat atggataatt ttcagggtaa
     3421 cgcggttgta aattatacgg aatatgaggt gccttataca aggattatcg aacacaagca
     3481 ttttgagttc ggcacgcagg aaaaaacgat aattacaaaa gagtatcctg cagattggaa
     3541 aaagggcgat gaaccctatt atccgatgaa tgatgaaaaa aacaatgcct tatttaaaaa
     3601 gtacaaagcg ctggcagaaa aagaaacaag ggttattttc ggcggacgtc ttggagaata
     3661 taaatattat gatatgcacc aggttgtagc aagggcgctt gaagcagcgt caggagaact
     3721 gtatgattga tacggaggga gttatggatg atgatatcag agttcagcaa atagaagcac
     3781 tgaggatggc ggacgagtat ttaaaaaagc tgattccaag tatagaagag gtaatcacgg
     3841 agcttgacgg tgaaatgaaa gaagacagtg tggattatct tgagcagatt gttgaaggct
     3901 ttaactttat gatcgagaca tttaacgtga caatggatct gatcaatgaa gaaagggtaa
     3961 tcatagataa ggatgatatt aaccgtgctg tcctgaattt aagcgatgcg ctgctttcca
     4021 ataactataa gcaggcagct actatatt
//
LOCUS       DAOSYE010000070         4038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15399_length_4038_cov_2.685162, whole genome shotgun sequence.
ACCESSION   DAOSYE010000070 DAOSYE010000000
VERSION     DAOSYE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15399_length_4038_cov_2.685162"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLU43_01965"
     CDS             <1..625
                     /locus_tag="PLU43_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO27204.1"
                     /translation="SSMLVCELAGIQPGDTVIDVCAAPGGKALHAADLLQGTGLVDAR
                     DITEAKTELIREHVVRCGFSNIQVRIQDAKELTKESIACADVVLADLPCSGLGIIGRK
                     GYIKYHVTAEKLSSLIALQHEILKTVGQYVKPGGILMYSTCTIHRAENEETAAWIRQH
                     LPFEPVSLKESLPKGIQSLTASEGFVQLLPGIHETDGFFIAKFRKDG"
     gene            643..1689
                     /gene="rlmN"
                     /locus_tag="PLU43_01970"
     CDS             643..1689
                     /gene="rlmN"
                     /locus_tag="PLU43_01970"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOO27205.1"
                     /translation="MEKTDIRSLHYTELEKAFETLLEKKFRAKQVYDWLHCKQADDFS
                     EMTNLSKDLRKKCGDYFDIYTVRPVEILESQIDGTKKYLFRLHDDHIIESVFMRYEYG
                     NSVCVSSQVGCRMGCRFCASTIDGLNRDLTSGEMLGQIYGIMKETGERISHVVVMGSG
                     EPLDNYENFLKFITMLTDEHGLHLSQRNVTVSTCGLAAEIKRLAEEKLQITLAISLHA
                     PTDKKRSELMPITQKYPLAELMEACRYYFDRTGRRLTFEYSLIGGVNDTPEDAAALTG
                     LLRGLNCHINLIPVNPVKERSYIQSEREVIRAFKNKLEKNHLNVTVRREMGRDIDGAC
                     GQLRRSYMEDTRMC"
     gene            complement(1686..2516)
                     /locus_tag="PLU43_01975"
     CDS             complement(1686..2516)
                     /locus_tag="PLU43_01975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOO27206.1"
                     /translation="MNIDISKLKKIASGFTADVYQLDETKVLKLYYEGWEKICIENEY
                     HVDLLINSYSIKAPKTYEMVQAGNRTGIIFQKLQNTTMKDMISQNKKNSLYYAKILAR
                     EHFKINSILDEQHGLLPQKDAYQEIISSRTSLNKQQKEKLLHLLEELPSDNRICHGDF
                     HPLNLLFESDDIYIIDWVGALSGNPLADVAGSYMIIKLMGTETKKPAGFLKNIYSRIG
                     INSFANTYLKEYLTVSNYSRKELYEWIPIRAATYLDFGLPDHVNKKLQHMIDQSLQHI
                     "
     gene            2738..3331
                     /locus_tag="PLU43_01980"
     CDS             2738..3331
                     /locus_tag="PLU43_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOO27207.1"
                     /translation="MKYYATTYLTKAAIGGSLKELLNARALNKISVSEICKNCGISRK
                     TFYYHFTDIYDIVSWLFEQEIGVQEESRSAADGIRYALQFSQKNRGLCRNLMKDAAGY
                     EMTRKYLKSIASDAHDTLLHALCESAFSHVPDEFKEFLIDYYSEGFSAYIIKTINGKE
                     LDVEEIENAVCYLTTMMEITMPAVLQAMELRKISGQI"
     gene            3363..>4038
                     /locus_tag="PLU43_01985"
     CDS             3363..>4038
                     /locus_tag="PLU43_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine polyphosphate pyrophosphohydrolase"
                     /protein_id="HOO27208.1"
                     /translation="MKKLNDYLYSGDTVLKILQRYSEDLRQSSKESHNQIDLVHCNFL
                     LQIAELLQHNDFLTSQSQRIREFYKLMANEYPFLAFTFKGRIKSLIRAEAKFNGYIVE
                     YIYEYYIENGTYPSLPELKNYLNCFRDLIAYRIVISLPKCHLKEGKICADEEIKYLYD
                     VANQLLDFMEERGFSAEMSSLTGQKRAPLLKESVSPYYRDYIANAESSGYRSLHITFY
                     DNIARCY"
BASE COUNT         1180 a          762 c          961 g         1135 t
ORIGIN      
        1 cagctccatg ctggtctgtg agcttgcggg cattcaaccg ggagacacgg tcattgatgt
       61 ctgtgcggcc ccgggcggca aagcgctgca tgcggcagat ctgctgcagg gaacaggact
      121 tgtggatgca cgggatatta cggaagcaaa gacagaattg atcagggagc atgtcgtacg
      181 ctgcgggttt tccaatattc aggtgcggat acaggatgca aaagaactga caaaggaatc
      241 gatcgcatgt gcggatgtgg tgctggcaga cctgccatgt tccggtcttg gaatcatagg
      301 caggaaaggc tatattaaat atcatgtcac agcagagaag ctcagctctc tgattgcttt
      361 gcagcatgaa attttaaaga cggtcggcca atatgtaaaa ccgggcggta ttctgatgta
      421 cagcacctgt acgatacacc gggctgaaaa tgaagagaca gctgcctgga tcagacagca
      481 tctgcctttt gagccggttt cattaaaaga aagcctgcca aaggggatac aatccttgac
      541 cgcatcggaa ggatttgtgc agctgcttcc cggaattcat gaaacagacg gtttttttat
      601 tgcaaaattc agaaaagacg gataggatgc caaggcaaaa ttatggaaaa aacagatatc
      661 aggtcattgc attatacgga gctggaaaaa gcatttgaaa cgcttttaga aaagaaattc
      721 cgcgcaaagc aggtttacga ctggctgcac tgtaaacagg cggacgattt ttcggaaatg
      781 acgaatcttt caaaggatct gcgtaaaaag tgcggagact attttgatat ttatacggtc
      841 agaccggtcg aaatactgga gtcccaaatc gatgggacaa aaaaatactt attcagactg
      901 catgacgatc atattataga gagtgtattt atgcgctatg aatacggtaa ttctgtctgc
      961 gtgtcgtcgc aggtcggctg caggatgggc tgtcgttttt gtgcgtccac gatagacggt
     1021 ctgaatcggg atttgacatc cggtgaaatg ctcggacaga tctacgggat catgaaagaa
     1081 acaggggaac gtatttctca tgtcgttgta atgggttccg gtgagccact tgataattac
     1141 gaaaattttt tgaagttcat cacgatgctg acagacgagc acggacttca tttaagccag
     1201 cggaacgtaa cggtatcgac ctgcggtctt gctgcggaaa taaaacgcct tgcggaagaa
     1261 aagctgcaga tcacgctggc aatttccctt catgcgccga cagataagaa gcgaagtgaa
     1321 ctgatgccga tcacgcagaa atacccgctt gcagaactga tggaagcatg caggtattat
     1381 tttgacagga cgggaagacg gctgacgttt gagtacagcc tgatcggagg cgtgaacgac
     1441 acacctgagg atgcggctgc gcttacgggg cttttaagag gactgaactg ccatataaat
     1501 ctgatacctg tgaatccggt caaagagcgc agttatatac agtcggagcg tgaagtcatc
     1561 agggcattta aaaataagct cgaaaaaaat catttaaatg ttacggtcag acgtgagatg
     1621 ggccgcgaca tcgacggcgc ctgcggtcag cttcgaagaa gctatatgga agataccagg
     1681 atgtgttaaa tatgctgcag cgattgatcg atcatatgct gcagtttttt attgacatga
     1741 tcgggcagtc cgaaatccaa ataggtggcg gctctgatcg gtatccactc atacagttcc
     1801 ttgcgagaat agttggaaac agtcagatat tcctttaaat aggtgtttgc gaatgagttg
     1861 attccgatac ggctgtatat atttttcagg aagccggccg gtttttttgt ttctgtgccc
     1921 atcagcttta tgatcatata ggagcctgcg acatctgcca gcgggttgcc gcttaaggca
     1981 ccgacccagt ctatgatgta aatatcgtct gattcaaata acagattcag gggatgaaaa
     2041 tccccatggc agatcctgtt atcggatgga agctcttcaa gtaaatgaag cagtttttct
     2101 ttttgctgtt tatttaaaga agtcctgctt gaaatgattt cctgatatgc atctttctgc
     2161 ggcagaagcc catgctgttc atctaagatg ctgtttattt taaaatgttc tcttgccagt
     2221 attttcgcat aatatagact gtttttttta ttttggctga tcatatcttt catggtagta
     2281 ttttgaagct tttgaaagat gatgcccgtg cgattgcctg cctggaccat ttcatatgtt
     2341 tttggagctt taatggaata ggaattgatc agaagatcca catgatactc gttttcaata
     2401 caaatttttt cccaaccctc ataatacagc tttaatacct ttgtttcatc taactgataa
     2461 acatccgcag taaatccgga tgctattttt tttagttttg agatatcgat attcatactg
     2521 tccctccgtt tttttggctg acacgatttt gcctgtctgc tatataaagt atagaatata
     2581 cgaaaaataa ataaaacaca caatcctgtt atgatgtatc aaatttacac agattcagga
     2641 aattgtttct tttttctttt ggttttttac tgaatcgatt ataatagata tgtgcggata
     2701 ccattttaca gtttattttt gcagcggagg aacgcggatg aaatattatg ccacaacata
     2761 tcttacgaag gcagccattg gcggaagctt aaaagaactg ctgaacgcaa gggcactcaa
     2821 taaaatatca gtcagtgaaa tatgtaagaa ctgcgggatc agcagaaaga cgttttacta
     2881 tcattttacg gatatttatg atattgtttc gtggctgttt gagcaggaaa taggggtgca
     2941 ggaggagagc cggagtgccg cagacggtat tcgctacgca ctgcagttca gccaaaagaa
     3001 cagaggctta tgcaggaatc ttatgaaaga tgcggctgga tatgaaatga cgaggaaata
     3061 tttgaaaagc attgcttcag atgcacatga tacgcttctg catgcactat gtgaatctgc
     3121 gttttcacat gtgccggatg agttcaaaga gtttttaata gactattaca gtgaaggatt
     3181 ttcagcatat attataaaaa cgatcaatgg aaaagaactg gatgtggagg aaatagaaaa
     3241 tgcggtctgt tatcttacca caatgatgga gatcacaatg ccggccgtat tacaggcaat
     3301 ggagcttcga aagatatcgg gtcaaatttg attttcagac atttgggaaa ggaaaagcag
     3361 ctatgaaaaa attaaatgat tatttatact ccggagatac cgttcttaaa attttacaga
     3421 gatattccga ggatcttagg cagtcgtcga aagaatccca taatcagatc gatcttgtac
     3481 actgtaattt tctgcttcag atcgcggagc ttttacagca taacgatttt ctgacgtcac
     3541 agtctcagcg gatccgggaa ttctacaagc ttatggcaaa tgaatatccg tttcttgcat
     3601 ttacttttaa gggaaggatc aaatctctga tacgcgcgga agcaaaattt aacggataca
     3661 ttgtagaata tatttatgaa tattacatcg aaaacggaac ctatccgtcc cttcctgaac
     3721 tgaaaaatta tttaaattgc ttccgggacc tgattgcata caggatcgtg atctctcttc
     3781 caaaatgtca tctgaaagag gggaagatct gtgcggatga agaaataaaa tatctgtatg
     3841 atgttgccaa tcagctgctt gattttatgg aagagcgcgg atttagtgcg gaaatgtctt
     3901 ctttgacagg gcagaaaaga gcgcccctgt taaaggaatc cgtaagtccg tactatcggg
     3961 attatattgc aaatgcagag tccagcggct atcgttccct gcatatcacc ttttatgata
     4021 atattgcccg ctgctatg
//
LOCUS       DAOSYE010000071         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15440_length_4032_cov_3.219764, whole genome shotgun sequence.
ACCESSION   DAOSYE010000071 DAOSYE010000000
VERSION     DAOSYE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15440_length_4032_cov_3.219764"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..443
                     /locus_tag="PLU43_01990"
     CDS             63..443
                     /locus_tag="PLU43_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO27209.1"
                     /translation="MKITHVGIYTSDLERMKVFYEKYFQAVGNEKYENKSGFSSYFMT
                     FESGARLELMSHKDLITREIVDKMKGIAHLAFSVGSREAVDALVKQLTEDGYCLLSPV
                     RETGDGYYEGCIADPDGNRIEITI"
     gene            486..1409
                     /locus_tag="PLU43_01995"
     CDS             486..1409
                     /locus_tag="PLU43_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO27210.1"
                     /translation="MKKTASKKFKRKTFIKTGMILLLLVAGLIAYPGGSTEEQEAENS
                     IGTEDSDLEVHFIDVGQGDSTLIKSGEHAMLIDAGDDSKGTKVQNYLKKQGVTELDYL
                     ILTHPDTDHIGGAPVIITKFSIDNLFMSDYEKDNKSYKKLLQSLADKSLTWSTPDVGA
                     VYTLGTASFTVIAPNKTYSDPNNSSVGILLKNGRNRFVFTGDAEEEAESDIVANGIDI
                     TADVYQVGHHGSRTASSQEFLSAMDPEYAVISCGEGNSYGHPHAATLNSLRAMGVKVF
                     RTDEQGSIIAVSDGTDITWNCAPSETWQTGE"
     gene            1492..2001
                     /locus_tag="PLU43_02000"
     CDS             1492..2001
                     /locus_tag="PLU43_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27211.1"
                     /translation="MNTTAGDFQKEFMLQMNKENAKRLQVIAVVLFLVAAFQAVIYSF
                     SDNNMFTDGLIKTKWIIIVLGVFVIGALQLVKRGVKQAVDFSGSIIFISAFLMIFFSV
                     INTFYAQGISNDISIYLLVLLAVIAANRMDFKSTLIMLAVNYFVFAVGMRFFQSDPRY
                     LFSHRSMVP"
     gene            2061..2585
                     /locus_tag="PLU43_02005"
     CDS             2061..2585
                     /locus_tag="PLU43_02005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO27212.1"
                     /translation="MDKMDIDQKNKKLKELSERDDLTGLYNRRTIYLRLTDLIKEAEK
                     TKKNIYLGILDLDHFKEINDRYGHLYGDEVLKTVAGKILENIREEDIAGRYGGDEFVI
                     ILYEVDYESAGTMMKKLLQEVNELDFDTYKLSFSCGIAAWNGESSEELFERADAYMYH
                     VKKLGKNDIQIERT"
     gene            2596..>4032
                     /locus_tag="PLU43_02010"
     CDS             2596..>4032
                     /locus_tag="PLU43_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOO27213.1"
                     /translation="MARQSFDYDWEFTERFEETLCGASVPAGKLESIDLPHSNTKMPY
                     HYFPVSCYQMVCGYRKLFFAENTWKGRRVFLTFEGVAHAAEVYLNAQKIEEHQGGYTS
                     FCVELTKQLLYGEKNVLAVKVDSRESLDIPPFGNVIDYMTYGGIYRSVILEVKETVYI
                     QDVYIQTPEVLPEKKKIDVQITVNEEAEHLTVRQSILDGTNRIHVQEAPVTGLVTKMT
                     AELLPVRLWDIEDPKLYSLLTVLYKDGKELDRKETVFGFRTCEFRKDGFYLNEKRIKI
                     RGLNRHQCYPYCGYAMPKAMQEEDARILKEELAVNAVRTSHYPQDQSFYDACDRLGLL
                     VFTELPGWQHIGGEAWKETACASVREMVLQNRNHPSVILWGVRINESQDDDEFYKKTN
                     QIAHQLDPSRQTSGVRFFHKSSLLEDVYAYNDFSHTGDNPGVEEKKEVTPDPDQAYLI
                     SEYNGHMFPTKAFDDEGHRLAHALRHARV"
BASE COUNT         1259 a          648 c         1039 g         1086 t
ORIGIN      
        1 agtagcaaaa aaaataaaaa gtactgataa atcggtactt gacttaaaga ggaggttatt
       61 atatgaaaat cacacatgtg ggaatttata catcggattt agaaagaatg aaagtattct
      121 acgaaaaata ttttcaggct gtggggaatg aaaaatatga gaataagagc ggattttcca
      181 gctattttat gacatttgag agtggtgcca gactggaatt gatgtctcat aaggatttga
      241 ttacaagaga gatcgttgat aagatgaaag ggatcgctca tcttgcattt tcggttggaa
      301 gcagggaggc tgtggatgcg cttgtaaagc agctgaccga agacggatac tgtttgttaa
      361 gtcctgtcag ggaaaccgga gacgggtatt atgaaggctg tattgcggat cctgacggaa
      421 acagaataga aattacgata taaaattcat cagcgggtaa atttttagaa tagtgggaaa
      481 cagaaatgaa aaaaacagca tctaagaaat ttaaacgtaa gacattcata aaaacaggta
      541 tgatcctgct tctgctggtt gccggtctga ttgcttatcc aggtggaagc acagaagagc
      601 aggaagcaga aaattctatc ggaacagagg attcggatct ggaggtgcat tttattgacg
      661 tcgggcaggg agactctaca ttgatcaaaa gcggagagca tgcgatgctg atcgatgccg
      721 gcgatgattc aaagggtaca aaggtccaga attatttgaa gaagcaggga gtcacggaac
      781 ttgattattt gattctgaca catccggata ccgaccacat aggtggtgcg cctgtgatca
      841 ttacgaaatt ttcaattgat aacctattta tgtcggatta tgaaaaagat aataagagct
      901 ataaaaaatt attgcagtcg cttgcggata aatctcttac gtggtcaacg ccggacgttg
      961 gagcggtata tacgcttggg actgcttcgt ttactgtgat tgcgcctaat aaaacctata
     1021 gcgatccaaa taattcctct gttgggatat tattaaagaa tggaaggaat cgttttgtat
     1081 ttacgggcga tgctgaagag gaggcggaaa gtgatattgt ggcaaatgga attgacataa
     1141 ccgcagatgt ttatcaggtc ggacatcacg gaagcaggac ggcaagcagc caggagtttt
     1201 tgtccgcaat ggatccggag tatgcggtta tcagctgtgg ggaggggaat tcttatggcc
     1261 atccgcatgc cgcaacatta aacagcttac gggcaatggg cgtgaaagtt ttcagaacag
     1321 atgagcaggg cagtatcatt gcagtatctg acggaaccga tatcacatgg aattgtgcac
     1381 cttctgaaac atggcagaca ggagaataaa gcagatgaac ggattataaa aagtatagta
     1441 ttatagaacg ttgtacagta taatgttgaa taggaaaaga ggacataatg aatgaatact
     1501 acagcagggg attttcagaa agagtttatg ctgcagatga ataaggaaaa tgcaaagaga
     1561 ctgcaggtga tcgctgtcgt gttatttctg gttgcagctt ttcaggctgt aatttatagt
     1621 ttttctgata acaacatgtt tacggacgga ctgatcaaaa caaaatggat tatcatcgta
     1681 ttaggcgtgt ttgtcattgg ggcattgcag ttagtaaaga ggggtgtaaa acaggcagtt
     1741 gatttttcag gcagtattat ttttatatct gcctttttaa tgatattttt ttctgttatc
     1801 aacacatttt atgcacaggg gatttctaat gatatttcta tttatctgct tgttctgctg
     1861 gctgttattg cagcgaatcg aatggacttt aagtcaacac tgatcatgct tgcggtcaat
     1921 tattttgttt ttgcagttgg aatgcgtttt tttcaaagcg atccgagata cctgttctct
     1981 caccgttcaa tggtgccatg agtactgtcc tggcttttat catatcaaga atgttttacc
     2041 gatacggtat gaaagacttt atggataaaa tggatattga tcagaaaaat aaaaaattaa
     2101 aggagctgtc tgagagagat gatctgacgg gattatacaa tagaagaacg atttatttac
     2161 gtttgacgga tctgattaaa gaagcggaaa aaactaaaaa aaatatttat ctggggattt
     2221 tggatctgga tcattttaag gagatcaatg acagatacgg acatctttat ggtgatgaag
     2281 tacttaagac agttgcagga aaaattcttg aaaacatcag agaagaagat attgctggca
     2341 gatatggcgg agatgaattt gttataattt tatatgaggt agactatgaa tctgccggga
     2401 ctatgatgaa aaaacttctg caggaagtca atgagctgga ttttgacacg tataaattgt
     2461 cttttagctg cggtattgca gcctggaatg gtgaaagcag tgaggagctt tttgaaagag
     2521 ctgatgcgta tatgtatcat gtgaaaaagc ttggcaagaa tgatatacag atcgagagaa
     2581 cataaagggg agactatggc aaggcagagc tttgattacg attgggaatt tacagagcgg
     2641 tttgaggaga cgctgtgcgg cgcatcggtt ccggcaggga aactggaatc gatcgatctg
     2701 ccgcattcca atacaaaaat gccgtatcat tattttccgg tatcctgtta tcaaatggtc
     2761 tgcggttaca ggaagctttt ttttgcagaa aatacatgga agggcagacg tgtgtttctg
     2821 acatttgaag gtgttgcgca tgcggcagag gtttatttaa atgcacagaa aatagaggag
     2881 catcagggag gctatacgtc cttttgtgtc gaactgacaa aacagctgct gtacggagag
     2941 aagaatgtcc tggcagtgaa ggtcgacagc cgggaatcgc ttgatattcc accctttgga
     3001 aatgtgatcg attatatgac atacggcggg atctaccgtt cggtcattct ggaagtgaag
     3061 gaaacggttt atattcagga tgtttatatt caaacaccgg aagtattgcc ggaaaagaaa
     3121 aaaatagatg tacagatcac tgtgaatgaa gaggcagagc atcttacggt cagacaaagc
     3181 attctggacg gaacaaacag gatccatgta caggaggcgc cggtcacagg acttgtcaca
     3241 aagatgacgg cagagctttt gccggtccgg ctctgggata ttgaagatcc aaaactgtat
     3301 tccctgctta cagtattgta taaggacgga aaagaactgg atcgtaaaga aacagtgttt
     3361 ggattcagga catgtgaatt tcgaaaagac ggattttatc tgaatgaaaa aaggataaaa
     3421 atacggggtc tgaacaggca ccagtgttat ccatactgcg gttatgcaat gccgaaagca
     3481 atgcaggaag aggacgccag gatcctgaaa gaagagcttg cggtaaatgc ggtcagaacc
     3541 tcacattatc cgcaggatca gtctttttat gatgcctgtg acaggctggg tctgcttgta
     3601 tttacagagc ttcccggatg gcagcatatc ggcggggagg cgtggaagga aactgcctgc
     3661 gcgtctgtga gggaaatggt tttacagaac agaaaccatc cgtccgtgat cttatgggga
     3721 gtgcgcatta acgaatcaca ggacgacgat gaattttata aaaaaacaaa tcagatcgct
     3781 catcagctgg atccgtccag acagacgagc ggtgtccggt tttttcacaa aagcagtctg
     3841 ctggaagatg tttatgcata taatgatttt tcccataccg gagacaatcc gggagtggaa
     3901 gagaaaaaag aggtgacgcc tgatccggat caggcctatt tgatttccga atataacgga
     3961 catatgtttc cgacaaaagc gtttgacgat gaagggcatc gtcttgcaca tgcactgcgg
     4021 catgccaggg tg
//
LOCUS       DAOSYE010000072         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15465_length_4027_cov_3.484139, whole genome shotgun sequence.
ACCESSION   DAOSYE010000072 DAOSYE010000000
VERSION     DAOSYE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15465_length_4027_cov_3.484139"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1482)
                     /gene="clpX"
                     /locus_tag="PLU43_02015"
     CDS             complement(217..1482)
                     /gene="clpX"
                     /locus_tag="PLU43_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOO27214.1"
                     /translation="MSGKTNDDKIRCSFCNKTQDQVRKLIAGPDGAFICDECVEICMD
                     IIEEEFEQEETNDNDRNPDINLLKPQEIKSFLDENVVGQEEAKKVLSVAVYNHYKRVT
                     SAQKSDVELQKSNIIMLGPTGCGKTLLAQTLAKIINVPFAIADATALTEAGYVGEDVE
                     NILLKVIQAADYDIERAQYGIIYIDEIDKITRKSENVSITRDVSGEGVQQALLKIIEG
                     TVASVPPQGGRKHPHQELIQIDTTNILFICGGAFEGLDKIVEARLDKKSIGFNAEIKD
                     KKESELSVLLSQVQPEDLVKFGLIPEFVGRVPICVSLEALDEKALVKILTEPKNAITK
                     QYKKLFELDGVSLDFEKEAIFEIAKRAYSKKTGARGLRAILENMMTDLMFKIPSDDTI
                     KQCVITKEAVDGTGAPLLVHYEDSLKKAE"
     gene            complement(1489..2073)
                     /gene="clpP"
                     /locus_tag="PLU43_02020"
     CDS             complement(1489..2073)
                     /gene="clpP"
                     /locus_tag="PLU43_02020"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOO27215.1"
                     /translation="MSLVPYVIEQTSRGERSYDIFSRLLKERIIFLGEEVNATTASLI
                     VAQMMFLEAEDPDKDIHFYINSPGGSVTDGFAIYDTMHYVKCDVATYCMGMAASMGAF
                     LLAGGTKGKRFALPNSEVMIHQPLGGAKGQATEIEIAAKQILRTKETINRILSENTGN
                     PIDIIAADTERDNWKSAQEALEYGLINKIITTRM"
     gene            complement(2128..3417)
                     /gene="tig"
                     /locus_tag="PLU43_02025"
     CDS             complement(2128..3417)
                     /gene="tig"
                     /locus_tag="PLU43_02025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO27216.1"
                     /translation="MSLKVEKLEKNMAKLTIEASAEDFEKAIEAAYHKNKGKMSVPGF
                     RKGKVPRAMIEKMYGTGVFYEDAANALIPEAYEKELSENKELEVVSQPKIDVVQIEKG
                     KPFIFTAEVAVKPEIELGKYKGVKVEKEDMEVTDEEIMKEIDKERESNARTINVEDRA
                     VQDKDMTIIDFEGFIDGVAFEGGKGENHPLTIGSGAFIPGFEEQLIGAELNKETEVKV
                     AFPEEYQAKELAGKDAMFKVTVKEIKEKELPEVDDEFAAEVSEFDTLEEYKADIKTKL
                     TDKKTADAKAKKEDAVIEAIIKDSNMEIPDPMLDTQTKQMAQDYAGRLQQQGLSLEQY
                     FQFTGMDMNKFMEQMRPGALKKIQSRLVLEAVVKAEDIKVSDEEFDAELAKMAEMYQM
                     EKEKLQEMIGDYEKEQISEDIAIQKAVEFVVSNAKEK"
BASE COUNT          952 a         1082 c          694 g         1299 t
ORIGIN      
        1 aagaaagaca tgctgatctt tcagcattgc tttttccaca gcgtataccg aatgttctct
       61 gttcaaatca aaatgaataa tcatccccgg cattacggta agtcctctaa gcgcaactgc
      121 cggcattttt ttcttcataa ctcttgccat acatactccc tgtaaattat acacataacg
      181 ccgccttgcg gcggcgtcca gatttttccg aatattttat tccgccttct tcagtgaatc
      241 ttcataatgc actaaaagcg gagcccccgt accatctacg gcttcctttg taattacaca
      301 ctgcttgatc gtatcatccg acggaatctt aaacatcaga tccgtcatca tattttccag
      361 tatggcgcgc agacctctcg cccccgtctt tttggaatat gcccgctttg caatctcaaa
      421 aatcgcttct ttctcaaaat caagacttac cccatccagc tcaaacagct tcttatactg
      481 ctttgtgatc gcatttttcg gttctgtcag aatctttacg agtgctttct catccagcgc
      541 ttccagtgaa acgcagatcg gcacacgccc tacaaactcc ggaatcagac caaatttcac
      601 aagatcctcc ggctgtacct gtgaaagcag cacacttaac tcgctctctt ttttatcttt
      661 gatctctgca ttaaagccga tcgacttttt atccagcctt gcctcaacga tcttgtcaag
      721 cccctcaaaa gcgccgccgc agataaacag aatatttgtc gtatcgatct gtatcagctc
      781 ctgatgcgga tgtttcctgc ctccctgcgg aggtacggaa gcaaccgttc cctcaatgat
      841 cttaagaagc gcctgctgca caccttctcc ggatacgtct cttgtaatgg aaacattctc
      901 ggattttctt gtgatcttat cgatctcatc aatataaata ataccatact gcgcacgctc
      961 gatatcataa tccgcagcct ggatcacctt taataaaata ttttcaacat cttcgcctac
     1021 ataacctgct tccgtaagcg cagtcgcgtc tgcgatcgca aacggcacat tgatgatctt
     1081 cgcaagtgtc tgcgccaaaa gtgtctttcc gcagcccgta ggacccagca taataatatt
     1141 gctcttctgc agctccacat ctgatttctg cgcggaggtc acacgcttat aatgattata
     1201 gacagctacg gacagcactt ttttggcttc ctcctgaccg actacgtttt catcaagaaa
     1261 actcttgatc tcctgtggct tcagcagatt aatatccgga ttcctgtcgt tatcgttcgt
     1321 ttcctcctgc tcgaattcct cttcaatgat atccatgcag atctccacac actcatcgca
     1381 gataaaagca ccatcaggtc ctgcaatcag tttgcgtacc tgatcctgtg tcttattgca
     1441 gaaagaacac cttattttat catcgttcgt ttttcctgac atatcttttt acatcctcgt
     1501 tgttatgatc ttattgatca gaccgtactc caaagcttcc tgcgccgatt tccagttatc
     1561 acgctcggta tccgccgcta tgatgtcgat cgggttgcct gtattctccg ataagatccg
     1621 attaatggtt tcctttgtcc ttaaaatctg ttttgcagcg atctcaattt ccgtcgcctg
     1681 cccttttgct ccgccaagcg gctggtgaat cataacttcg gaattcggca gggcaaaccg
     1741 cttgcccttt gtgccgcccg caagtaaaaa tgcacccatg ctggctgcca tgcccataca
     1801 ataggtagcc acgtcacatt tcacataatg catcgtatca tagatcgcaa aaccgtccgt
     1861 gaccgaaccg ccgggactgt tgatataaaa atggatgtcc ttgtccgggt cttccgcctc
     1921 taaaaacatc atctgtgcta cgatcaggct ggcggtcgtt gcattcacct cttctcccaa
     1981 aaagataata cgttccttta acaatctgga aaaaatatca taagaccgct ccccgcggct
     2041 cgtctgttca atgacatatg gcactaaact catttttgtt cctcctaaat ctgcttctct
     2101 gaataccgct gtatccctgt tatctgttta tttttctttt gcattggaaa caacaaactc
     2161 tacggctttc tggatcgcaa tatcttccga gatctgctcc ttctcataat caccgatcat
     2221 ttcctgcagt ttttccttct ccatctgata catctcggcc atcttcgcaa gctccgcatc
     2281 gaattcctcg tcggaaactt tgatatcttc agcctttaca actgcctcca gtacgagtct
     2341 gctctggatc tttttcagcg cacccggcct catctgttcc atgaacttgt tcatgtccat
     2401 gcctgtaaac tggaagtact gctctaaaga caggccctgc tgctgcagtc tgcctgcata
     2461 atcctgagcc atctgctttg tctgcgtatc cagcatcgga tccggaatct ccatattgga
     2521 atccttaatg atcgcttcga tgaccgcatc ttctttcttt gctttggcat cggctgtctt
     2581 tttatccgta agttttgttt taatatctgc cttatattcc tcaagcgtat caaattcaga
     2641 aacttccgct gcaaactcat catcaacttc cggcagttcc ttttccttga tctccttaac
     2701 cgttaccttg aacatcgcat cctttccggc aagctctttc gcctgatact cttccggaaa
     2761 agcgactttg acttctgttt ccttatttaa ttccgctccg atcagctgct cctcgaagcc
     2821 tggaataaac gcaccggagc cgatcgtcag cggatgattt tcccctttgc cgccttcaaa
     2881 tgcaacaccg tctataaagc cttcaaaatc aatgatcgtc atatctttat cctgaacggc
     2941 cctgtcttcc acattgatcg tccttgcgtt gctttctctt tccttgtcga tctccttcat
     3001 gatctcttca tcggttactt ccatatcttc tttttcaact ttgacacctt tatatttgcc
     3061 aagctcgatc tccggcttta ctgccacttc tgccgtgaaa ataaaaggct ttcccttttc
     3121 gatctgtaca acatctattt tcggctgtga tacgacttcc agctccttat tttcggaaag
     3181 ctctttctcg tatgcttcag gaatcagtgc attggcagca tcttcataaa aaactccggt
     3241 accatacatt ttttctatca ttgcacgggg taccttgcct tttctgaaac caggtacact
     3301 cattttgcct ttattcttat gataagcagc ttcaatcgct ttttcaaaat cctcggcaga
     3361 tgcttcgatt gtaagctttg ccatattctt ttctaatttc tcaaccttta aactcatttt
     3421 actttcgttc ctccttaatt tctgtttccg ccggtcggtt ttttcggatt ccggatatta
     3481 cagtcattat aagattatat cacacacatg tttaaaattc cagtgaaaaa gtattaaaag
     3541 tgcgtaaaat atagggttgt ctaatttccc gtatacgtat ataatattat acgaaaagtg
     3601 cctgctttgc actttatcat ttagttttca aactctcagc ccggcatata gactggattt
     3661 atattttggc ctgaccgttt tcgttttcat ataatcattc attttctgaa acaaaggatt
     3721 cctgcgctaa gaaggtggaa catatttttg ctgttttgtt tccccctaca atgaatcacg
     3781 cgtgctcctt ctgttctcgg aaggggtttt tttatataaa acagacgatc ctgtatgtat
     3841 ttgtcggtat gacagaaagg aggatccatg gagaataaaa tcgccgttat cggaatcttt
     3901 atccgcgata tgcaggcagc ggccgccgtt aacgaaattc tgcacgactt tgccgataag
     3961 gtcgtcggca gaataggaat tccgtaccgg gaaaagaatc tgcatatcat ctccgtcatt
     4021 atgaatg
//
LOCUS       DAOSYE010000073         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15494_length_4022_cov_3.403075, whole genome shotgun sequence.
ACCESSION   DAOSYE010000073 DAOSYE010000000
VERSION     DAOSYE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15494_length_4022_cov_3.403075"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLU43_02030"
     CDS             <1..659
                     /locus_tag="PLU43_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO27217.1"
                     /translation="NTQILCVLNGVDSEEILIREFGEAHVLYSYMRMSVVMKDGVADF
                     DPYWGKICFGEKQNKIYSERVEKVKEVFEDSDIPCTIEEDMLKGIWFKYMCNIGENMT
                     CALFGIPFGAFRRSADANAVRVSAMREVAAIAQKKGIDIGETEIKLQENTVKTIPYTN
                     KPSTLQDLEAGKKTEIELFAGTVVRMGKELSIPTPINEIFYHGIRVLEEKNEGLCEVN
                     "
     gene            661..1431
                     /locus_tag="PLU43_02035"
     CDS             661..1431
                     /locus_tag="PLU43_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO27218.1"
                     /translation="MFSIAICDDDKKALDELKKKLENNKEFPKNAQIYAYQSGKELIE
                     EKAEFDLIILDMQMKELDGRETARRLRNEKGEGFILGFFTGKEAPNSRDFLLKPSRYI
                     MKFSEEEEILEDIGALLLEMKDRQREEYFLCTYGGKTVKLNIKDILYISNSKNGAEVA
                     ICKWNAGKMETEVCKCNKKLNQIFAELPQGRFTHVQKLFLGNMEHIIKIHGNQIWFAN
                     NMPVNLSRTYKEEFKKNLAQYHRRYFSMKGNTETDSDD"
     gene            1424..2737
                     /locus_tag="PLU43_02040"
     CDS             1424..2737
                     /locus_tag="PLU43_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO27219.1"
                     /translation="MIEIIYNIPISIFEIYMFYDFFQGGFRYRNEKKKIHLFVLIGLF
                     FAITFVNAFQNSVINILLLPFLYFFASILLFREPVKKLLFHNLIFYIIMAGIEVGFEV
                     AFSIVSGDILISVYQEPVSRMMIVLFEKLISFLVLKAVKNSRRPRSFPMEKSLYRLVY
                     LLPFVSFLIYGGITYSEINLGVFSIQKGVLVLGCFLLVPINIVVFYLLERLSDTMEKT
                     KNLELAALKQELEEVHYERVDEINEEHKKYLHDMQHYFKMIGDLAQLGEDGEIIKIIK
                     DMDIEIEKIRRKRFCRHPIVNAMLCETEEKARRNEISYHVKMDPITDFSLIDDFSLIC
                     MTGNLLQNAFEAAVRSEQNRFIHIEAHLASNKKFIVFCIKNGFDDAPVLENGEYLTSK
                     QDKAKHGIGIRNVRERAESYGGFLITESEDHVFSATLELAAETSK"
     gene            2909..>4022
                     /locus_tag="PLU43_02045"
     CDS             2909..>4022
                     /locus_tag="PLU43_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27220.1"
                     /translation="MKKIQLFLQGAIIFMLACTAYLCGAYVTEYRNIQQDNESTVATI
                     AVVNTDNGVTADGEYINYAEKLISYPDVNFEAASLTEAREGIENDRYAAFILIPGTFS
                     ESIQSVNTKPQKANITYAVNSNLREDVKIKTVNDIHNFILNLSTNISYIYVDSILKEV
                     HAVQDDSDAIMENDTKDMDDRAGNLIRTEDALGRTRELTYNKRGKAETIRQADGSELT
                     FTYDAHGNTQSITDAYGTTLTYKYDALNRLVSAGDNSGQRLQLAYDRKDFVTAVTNAA
                     GSRREYQYDENGNPVCITDFDGSIIRREYNALSLLSKETDAEGNETEYSYDTMWNIKE
                     IKRELLFYLDPMTGESNILFQTENESTRIVGYEDGDI"
BASE COUNT         1343 a          646 c          992 g         1041 t
ORIGIN      
        1 ctaatacgca gatcctgtgt gtactgaacg gagtggattc tgaggagatc ctgatcagag
       61 aatttgggga agcgcatgtt ctttattctt atatgcggat gtctgttgta atgaaggatg
      121 gagttgctga ttttgatcca tattggggaa agatctgttt tggggaaaag cagaataaaa
      181 tatattcgga acgggtggaa aaagtaaaag aagtatttga agacagcgat attccctgca
      241 cgatcgaaga agatatgctg aaagggatct ggtttaagta tatgtgcaat atcggggaaa
      301 atatgacgtg tgcgctgttt ggcattccgt tcggtgcttt caggaggagc gcggatgcga
      361 atgctgtccg cgtaagtgca atgcgtgaag tagcggcgat cgcacagaaa aaaggaattg
      421 atatcggaga gacggaaatc aagctgcagg aaaataccgt aaaaacgatt ccttatacaa
      481 acaagccgtc tacactgcag gatctggagg ccggaaagaa aacagagatc gaactgtttg
      541 ccggaacagt agtccgtatg gggaaggagc tttccatacc gacgccgatc aatgagatct
      601 tttatcatgg gatccgtgtt ttggaggaaa aaaatgaggg actgtgtgag gtgaactgaa
      661 atgttttcga ttgcaatttg tgatgacgat aaaaaagctt tagatgagtt aaaaaagaaa
      721 ctggagaaca ataaagaatt tccaaaaaac gctcaaatat atgcgtatca gtccggaaaa
      781 gaactcatag aagaaaaagc agaatttgac ctgatcatat tggatatgca gatgaaagag
      841 ctggacggca gagagacggc aagaaggctg agaaacgaaa agggtgaagg atttattctg
      901 ggctttttta ccggaaagga agcgccgaat tcgagagatt ttttgttaaa accgagcagg
      961 tacattatga aattttcaga agaagaggag atcttagaag atattggagc tcttttgctg
     1021 gaaatgaaag accggcagcg ggaagagtat tttctgtgta cctatggcgg aaaaactgta
     1081 aagctgaata taaaagacat cctgtatatt tcaaattcga aaaacggagc agaagtggcg
     1141 atctgcaaat ggaatgcagg gaaaatggaa acagaggtct gcaaatgcaa taaaaaatta
     1201 aaccagatct ttgcagaact gcctcagggc agatttacgc atgtccagaa gcttttcctt
     1261 ggaaatatgg aacatattat aaagattcat ggaaatcaaa tatggtttgc gaataatatg
     1321 cccgtaaatt tatccaggac ctataaagaa gaattcaaga aaaatcttgc acagtatcat
     1381 cggcggtatt tttccatgaa agggaatacg gagacggata gtgatgattg aaattattta
     1441 taatataccg atcagcatat ttgagatcta tatgttttat gatttttttc agggcggttt
     1501 cagatatcgg aatgagaaga agaaaataca tctgtttgtg ctgatcggtc tgttttttgc
     1561 aattacgttc gtaaatgcat ttcagaattc cgtaatcaat attcttttgc tgccgtttct
     1621 gtattttttc gcgtccatat tgctatttag ggaaccggta aaaaaactgc tgtttcataa
     1681 cctgattttt tatattatta tggcaggtat tgaggttgga tttgaagttg cattcagtat
     1741 tgtttcagga gatatattga taagcgttta tcaggagccg gtcagcagga tgatgatcgt
     1801 tctgtttgaa aaactgatca gctttttagt gctgaaggca gtcaaaaaca gcagaagacc
     1861 cagaagcttt ccaatggaaa aaagtcttta ccggcttgtt tatctgctgc cgttcgtttc
     1921 ttttttgatt tatgggggaa tcacatattc ggaaatcaat ctcggcgttt tttctattca
     1981 aaaaggtgtg cttgtgctcg gctgcttttt gcttgttcca atcaatattg ttgtatttta
     2041 tttactggag agattgtcgg acacgatgga gaagacaaag aatcttgagc tggcagcatt
     2101 aaagcaggaa cttgaagaag tccattatga acgggtcgac gagatcaatg aggaacataa
     2161 aaaatatctg cacgatatgc agcattattt taaaatgata ggtgatcttg cgcagcttgg
     2221 cgaagatggg gaaatcataa aaataataaa ggatatggac atagaaatag aaaagatcag
     2281 aaggaaaaga ttctgccggc atccaattgt aaacgcgatg ctgtgcgaaa cggaagaaaa
     2341 agcacgtcga aatgagatat cctatcatgt caaaatggat ccgataacag atttttcatt
     2401 gatcgatgat ttttccctga tctgtatgac tggtaatctg cttcaaaatg cgtttgaagc
     2461 agctgtcagg tcggaacaaa acagatttat tcatattgaa gcacatttgg cttccaataa
     2521 aaaatttatc gttttttgta tcaaaaatgg atttgatgat gcgccggtac tggaaaatgg
     2581 ggaatatctg accagcaagc aggacaaggc aaagcatgga atcggaatca ggaacgtcag
     2641 ggagcgtgcc gagtcgtatg ggggattttt aataacggaa tcggaagatc atgtattcag
     2701 tgccacttta gaactggcag cggaaacgag caaataaata tccaataaat tacatatatt
     2761 tcatatcata gtaaaattca tgtaaaatca gacttaaatt gtgtaaaatt agcctatccc
     2821 attgcgtaat ttacaaaaat tagttaaagt aaaagaaagg aagcgaaggg gatttacgac
     2881 gctattacca aaataaggaa tgggaaaaat gaaaaaaata cagctgttcc tgcagggagc
     2941 aattattttt atgctcgcct gtacggcata tttatgcggc gcatatgtaa cagagtaccg
     3001 gaatattcag caggataacg aaagtacggt ggcaaccatt gcggtagtaa atacggataa
     3061 cggggtgacg gcagacgggg agtacataaa ttatgcggag aagctgatca gctatccgga
     3121 tgtaaatttc gaggcggcaa gcctgaccga agcgagagaa ggaattgaaa atgacagata
     3181 tgcggcgttt attttgattc cggggacctt ttcggagtct atccaaagtg tgaatacaaa
     3241 accgcagaaa gcgaatatca cctatgcggt caattcaaat ctaagagaag atgtcaaaat
     3301 aaaaacagtc aatgacatcc acaacttcat tttaaatctc agtacaaata tttcctatat
     3361 ttacgtggac tccatattaa aagaggtgca cgctgttcag gacgattcag atgccattat
     3421 ggagaacgat acaaaagata tggatgaccg tgcagggaac ctgatcagga cagaggatgc
     3481 cttggggagg acaagggaac tcacctataa caaaagagga aaggcggaaa caatcaggca
     3541 ggcggatgga agtgagctta catttactta tgatgcgcat gggaatacac agagcattac
     3601 ggacgcatac ggaacaacgc tgacctataa gtacgacgcc ttaaaccgtc tggtgagtgc
     3661 aggggacaac agcgggcagc ggctgcagct tgcctatgac cggaaggact ttgtcacagc
     3721 agtcacaaac gcggcgggca gccgcaggga ataccagtat gatgagaatg gaaatcctgt
     3781 ttgtataact gattttgacg gaagcataat ccggcgggaa tacaatgcac tttccttact
     3841 gtcaaaggag acggacgcgg aaggaaatga aacggaatac agctatgata cgatgtggaa
     3901 tatcaaagag atcaaaagag agctgctgtt ctatctagat ccaatgacgg gagagagcaa
     3961 catccttttc cagacagaaa atgaaagtac aaggatcgtt ggatatgagg atggagacat
     4021 ct
//
LOCUS       DAOSYE010000074         4018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15510_length_4018_cov_3.232400, whole genome shotgun sequence.
ACCESSION   DAOSYE010000074 DAOSYE010000000
VERSION     DAOSYE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15510_length_4018_cov_3.232400"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLU43_02050"
     CDS             <1..1335
                     /locus_tag="PLU43_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO27221.1"
                     /translation="LQVTRTGSDTTLSKIVKLMEDAQGKKAPISKLADTVAGYFVPAV
                     MFIAVAAAIVWALLGHDLAFVLTIFVSVLVIACPCALGLATPTAIMVGTGLGAGNGIL
                     IKSGEALEILHKANAVVLDKTGTITEGKPAAMELVCASGSADIRENQVLDDTMRHILM
                     IAASLEQVSEHPLGRAVVEAAARYDIVSEPVTDFVSITGQGIGGRIGSKLYYAGNKTL
                     CAWKQFSMGAYEAHAEELAGKGQTPIYIADDEQGVFGLVSVADPVRETSRSAVEKLHE
                     LGMQVYMLTGDHALTAGYIGDKVGVDRVISEVLPQDKASVIESLQKEGNCVLMAGDGI
                     NDAPALVQADIGVAIGNGSDIAIESGDVVLMKSDLTDVYKAVKLSRATIRNIRQNLFW
                     AFFYNSCGIPLAAGLFYAINGTLLSPVFAGLAMSLSSVTVVGNALRLRRLKL"
     gene            1381..1680
                     /locus_tag="PLU43_02055"
     CDS             1381..1680
                     /locus_tag="PLU43_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOO27222.1"
                     /translation="MEEEKCCVRKKHREETEYKDLVTRLNRIEGQVRGIKRMVEDERY
                     CVDILTQVTAIQAALNAFNKKLLNSHIHSCVIEDIKQDKEGAVDELCDIIQKLMK"
     gene            1707..1904
                     /locus_tag="PLU43_02060"
     CDS             1707..1904
                     /locus_tag="PLU43_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOO27223.1"
                     /translation="MMTTIQVPDMHCEKCVERITKLLTEDGLAFTVSLTDKSVTVDGC
                     DHCVKTAVEDLEDLGFTPSVQ"
     gene            complement(1961..2608)
                     /locus_tag="PLU43_02065"
     CDS             complement(1961..2608)
                     /locus_tag="PLU43_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HOO27224.1"
                     /translation="MINDEFEPDFSSDTDHISQEEYDELIKPYIEALTVIQLRLNSLN
                     EEYRSRSIDYPIHNIQNRIKSRKSITKKLMKKTRKITIESAKDSLTDIAGIRVICYFE
                     QDIYNVVSAIKKQGDLIVIKESDYVRCPKQNGYKSYHIVLGIPVFSLEETEYYPVEIQ
                     IRTLTMDLWASMEHRICYKNGHGIEVTKETKNMLLLYAQGLEKMEKDMQKQSIQA"
     gene            complement(2725..>4018)
                     /locus_tag="PLU43_02070"
     CDS             complement(2725..>4018)
                     /locus_tag="PLU43_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO27225.1"
                     /translation="PSKRSFAKNLDISVITVENAYAQLIAEGYIYSIPKKGFFVQAVT
                     GQPDVFRTASSDSKKTENKLPETSCAADFISNRNNPSNFPFSVWAKLMRETISERGSE
                     LMIPSPSAGVFELRTAICKHLHAFRGMSVVPSQVIIGAGTEYLYSLLIQLLGRDKIYA
                     VENPGYQKLSQIYESNGVACCYLPMDENGICLPALTSSDADVLHISPAHHFPTGIVTS
                     IVRRYELLGWAAGMDSRYIIEDDFDSEFRMHGKPIPMLQSIDVLEKVIYMNTFSKSLS
                     STIRVSYMVLPPHLAAAFHEKLGFYSCTVSTFEQYTLARFINEGYFEKHINRMRTYYR
                     TLQGKLLSSIKNTSLSSKVTILAENAGLHFLLRINTSLSDKELVAKAARSGLLVSCLS
                     QYCFDDEVADSHTLIINYSGIDTDILKKFPDLLRKCIS"
BASE COUNT         1063 a          798 c         1070 g         1087 t
ORIGIN      
        1 ctgcaggtga cacgtacggg ctctgatacg acactttcta aaatcgtgaa actgatggaa
       61 gatgcacagg ggaaaaaggc gccgatctca aagcttgccg atacggttgc cggatatttt
      121 gtaccggcag tgatgttcat tgcagttgca gccgcgatcg tctgggcgct gcttggacat
      181 gatctggcgt ttgttttgac gatttttgta tccgtgcttg tgatcgcctg tccgtgtgca
      241 cttggtcttg cgacaccgac tgcgatcatg gtcggcaccg gactcggagc agggaacggg
      301 atcctgatca agagcgggga agcactggag atccttcata aagcaaatgc agttgtactc
      361 gacaaaacag gaacgatcac ggagggaaaa ccggcggcca tggaactggt ctgtgcgtct
      421 ggaagcgcgg acatcaggga gaaccaggta cttgacgata cgatgcgtca catattgatg
      481 attgcggcat cgcttgagca ggtatcggaa catccgcttg gccgtgcggt cgtggaggct
      541 gctgcgcgct atgatatcgt gtctgaaccg gtgaccgatt ttgtgagcat tacgggacag
      601 ggcatcggag gcaggatcgg cagtaaactg tattatgccg gtaataagac tctttgtgca
      661 tggaagcagt tttcgatggg agcttatgaa gctcatgcgg aggagcttgc gggaaaagga
      721 cagacaccaa tttatattgc cgatgatgag cagggggtat ttggtcttgt cagtgttgcg
      781 gatccggtca gggaaacgag cagaagtgcc gttgagaagc tgcatgagct tggcatgcag
      841 gtctatatgc tgacaggaga tcatgcgctg actgcaggtt atattggaga taaagttgga
      901 gtggaccgtg ttatttccga agtgcttccg caggataagg catctgttat agaatctctg
      961 caaaaagaag gaaactgtgt tctgatggca ggtgacggga tcaatgatgc gcctgcactc
     1021 gtgcaggctg atattggtgt ggcgatcgga aacggcagcg atattgcaat tgaatccggg
     1081 gatgttgttt taatgaaatc agatctgacg gatgtataca aagcagtaaa gctcagcagg
     1141 gcaacgatcc ggaatatcag gcaaaatctg ttttgggcat tcttttataa cagctgcgga
     1201 attccgctgg ctgcaggatt gttttatgcg atcaacggaa cactgttaag tccggtgttt
     1261 gcaggtctgg ccatgtcgtt aagttcggtg accgtagtcg ggaatgcact gcgcttaaga
     1321 cggctgaagc tgtaatggca gtaggacgaa aaggtttaca taaagtggag gaactgttgt
     1381 atggaggaag aaaaatgctg tgtacggaaa aaacatcggg aggagacgga atataaagac
     1441 ctggtcacac gtctgaacag aatagaagga caggtgcgcg gaatcaaaag gatggttgag
     1501 gacgaacggt attgcgttga tattctgaca caggtcacgg cgatccaggc agccttgaat
     1561 gccttcaaca aaaaattgct gaacagccat atccattcct gcgtgatcga agatatcaaa
     1621 caggataagg aaggcgctgt agacgaatta tgtgatatta ttcagaaact gatgaagtaa
     1681 cgattgattt tcagagagga gcgtgtatga tgacaacgat tcaggtaccg gatatgcatt
     1741 gtgagaaatg cgtagagagg ataacaaaac tgctgactga agacggactg gcatttacgg
     1801 tcagtcttac ggataaatcc gttacggtcg acggctgtga tcactgcgtg aaaactgcgg
     1861 tggaggatct tgaagatctg ggatttacac cgtccgttca ataataaaaa cagaggaagc
     1921 ggatactgcc gattcctctg cttatgggca tatgggccga ttaagcctgt atgctctgtt
     1981 tttgcatatc tttttccatt ttttcaagtc cctgcgcata gagcaggagc atattttttg
     2041 tttcttttgt tacttcgatc ccatgcccgt ttttataaca gatccggtgc tccatgcttg
     2101 cccacagatc cattgtcagt gtgcggatct ggatctctac cggataatac tctgtttctt
     2161 ccagggagaa aacagggatg ccgagtacaa tatggtagct cttatagccg ttttgttttg
     2221 gacatctgac ataatcggat tccttgatca cgatcagatc accctgtttt tttatggcgg
     2281 acacaacgtt gtaaatatcc tgttcaaaat agcagataac gcgaatcccg gcaatatcgg
     2341 tgagagaatc ttttgcgctc tctatggtga ttttgcgggt ttttttcatt aatttttttg
     2401 taatgctttt tcttgattta atgcggtttt gaatattatg tattgggtaa tcgatactgc
     2461 ggcttctgta ttcttcattc aggctgttga gccgcagctg tatgactgtc aatgcctcaa
     2521 tatatggctt tatcagctca tcgtattctt cctgggaaat atggtccgta tcggaagaaa
     2581 aatccggttc aaattcatca tttatcaagt ggtatgcacc tccccaattt tttttcgtgc
     2641 agatacgatg acaatattta ttttaataaa aatatgtgaa aaaacaatgg caaatacatg
     2701 aatttaataa ttctttacca aattttaaga aatacatttt cgcagcaggt ccggaaattt
     2761 tttcaggatg tcggtatcaa tgccggaata gttgatgatc agcgtatggc tgtctgcaac
     2821 ctcgtcgtca aaacaatatt gggaaaggca ggaaaccaaa agtccgctgc gtgctgcctt
     2881 tgccaccagt tccttgtcgg acaggctggt gtttatacgg agcagaaaat gaagtccggc
     2941 gttttccgca aggatcgtca ctttggagga aagggaagta tttttgatcg aggacagcag
     3001 ttttccctgt aaggtacggt aatacgtgcg catacggttg atatgttttt caaaatatcc
     3061 ttcattgata aaacgagcaa gcgtatattg ctcaaaagta gacaccgtac aggaataaaa
     3121 gccgagtttt tcatgaaaag ccgccgcaag atgaggcggc agcaccatat agcttacccg
     3181 gatcgttgag gaaagacttt tggaaaaggt attcatataa atgacttttt cgagcacatc
     3241 aatgctctgc agcattggaa tcggttttcc atgcatacgg aattcggagt cgaaatcgtc
     3301 ttcaataata tatctggaat ccataccggc ggcccagcca agcagctcat aacgtctgac
     3361 gatcgatgtg acgatgccgg taggaaagtg atgggccgga gaaatatgca ggacgtcggc
     3421 atcggaggaa gtaagtgcag gcaggcagat gccgttttcg tccattggaa gatagcagca
     3481 tgcgacaccg ttgctttcat agatctgtga gagcttttgg tagccgggat tttcgactgc
     3541 atagatttta tctcttccaa ggagctgtat cagcagactg tagagatatt ccgtaccggc
     3601 gccgatgatg acctgggaag gaacgaccga catcccgcgg aaagcatgca gatgtttgca
     3661 gatagcggtc cgcagttcga aaacacctgc gcttggagaa gggatcatca gttcactgcc
     3721 gcgctcgctg atcgtttccc gcatcagttt tgcccagacg gaaaacggaa agttggaagg
     3781 gttgttgcgg ttgctgataa aatcggcggc gcagcttgtt tcaggcagct tgttttctgt
     3841 ttttttagaa tcagaagaag ctgtccggaa aacatccggc tgtcctgtga cagcctgtac
     3901 gaaaaatcct tttttcggga tggaatagat atatccttct gcaatcagct gagcatacgc
     3961 attttccact gtaatgacac tgatgtccag attttttgca aagctccgtt tcgatggc
//
LOCUS       DAOSYE010000075         4002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15597_length_4002_cov_3.034963, whole genome shotgun sequence.
ACCESSION   DAOSYE010000075 DAOSYE010000000
VERSION     DAOSYE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15597_length_4002_cov_3.034963"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..960
                     /locus_tag="PLU43_02075"
                     /pseudo
     CDS             1..960
                     /locus_tag="PLU43_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439396.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1188..2186
                     /locus_tag="PLU43_02080"
     CDS             1188..2186
                     /locus_tag="PLU43_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO27226.1"
                     /translation="MVYTDIGIDLGTASILVYIRGKGVVLKEPSVVAFDRDTNKIKAI
                     GEDARLMLGRTPGNIVAVRPLRQGVISDYTVTEKMLKYFIQKAIGKKSFRKPRISVCV
                     PSGVTEVEKKAVEDATYQAGAREVAIIEEPIAAAIGAGIDISKPCGNMIVDIGGGTTD
                     IAVISLGGTVVSTSIKIAGDDFDEAIVRYMRKKHNLLIGERTAEDLKIKIGAAFKRPE
                     VDYLEVRGRNLVTGLPRTVKVSSEETEEALRETTSQIVEAIHSVLERTPPELAADIAD
                     RGIVLTGGGSLLRGLEDLIAYKTGINTMTAEDPMTAVAIGTGKFVEFLAGYSDDVD"
     gene            2203..2991
                     /gene="flgF"
                     /locus_tag="PLU43_02085"
     CDS             2203..2991
                     /gene="flgF"
                     /locus_tag="PLU43_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOO27227.1"
                     /translation="MVKGLYTASTGMINEQRRVDVLTNNMANSATTGFKKEGTTSEAF
                     KDVLAYKIKDTSEPWGAKTLGTMNMGVKIGENYTDYSQGALHETGNTFDLALSGDGFF
                     AVEFTAKGEDGEAGETSVKYTRDGSFTLNADGELVTKDGDYLLDVDGDHIVLDPTQEF
                     SVDRMGNIMQDDENVGQIQITDFEDYDYLEKYGENYFQTVDGAAEQDADAAVYSGYLE
                     SSNVQIVQEMVDLITYTRAYETNQKMIQAFDETLSTAVNDVGKV"
     gene            3029..3850
                     /locus_tag="PLU43_02090"
     CDS             3029..3850
                     /locus_tag="PLU43_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOO27228.1"
                     /translation="MVRALWTAASGMIGQQDSVDTIANNLANVNTTAYKTESNEFKSL
                     LYQTIQTKTTTANGENKPVGAQVGLGVRTASVTSQFVQGTLVDSESDTAFAIDGKGFF
                     AVEGEDGNTYYTRNGNFLFSVGVNNKMTLSNSDGLPVLNTAGKPITIPANYVSSEVVV
                     DSDGQLWYPDDDGTLEEIAGMQIGVFQFQNPSGLEKMGDTLYAETAASGAALNEATND
                     NLSKSELYQGYLENSNVEVADEMVNLIIAQRAYEMNSKAVQTADTMMQEANELRR"
BASE COUNT         1215 a          707 c         1114 g          966 t
ORIGIN      
        1 agatattgac gttgagattc cacttggcgt tatgacttgt gtaacgggag tttccggttc
       61 cggaaaaagc tcactggtca atgagatatt atataaaaag cttgcacggg tattgaatcg
      121 tgcaaggacg attcctggaa agcaccggtc ggtcgaagga ttcgaacagc ttgataaggt
      181 gattgcaatc gaccagtcgc cgatcggacg gacacccgtt ccaatccggc aacctatacg
      241 ggtgtgtttg atcagatccg tgcattgttt gcatccaccg cagacgcgaa ggagcgcggt
      301 tacagcaaag gccggttcag ttttaacgta aagggcggca gatgcgaagc atgcagcggc
      361 gatggtatta ttaaaattga aatgcacttt ttgccggata tttacgtgcc gtgcgaagtc
      421 tgcggaggaa agcgttataa cagggaaacg ctggaagtgc gttacaaagg aaaaaatatt
      481 tatgatgtgc tgaatatgac tgtggaagag gcaatgacct attttgaaaa tctgccgtct
      541 atcagaagaa agatacagac actgtatgac gttggtttat cgtatatcaa gcttggacag
      601 ccgtctacaa cattgtcggg aggggaagca cagcgtatta agcttgcaac ggagttaagc
      661 cgcagaagta cgggcagaac gatctatatt ttggacgagc ctacaacagg actgcatttt
      721 gcggatgtcc ataagctgat cgaaattctg cataagcttg cggaaggcgg gaatacggtc
      781 gttgtgatcg agcataatct ggatgtgatc aaaacagcgg attatattat tgatatgggt
      841 cctgaaggcg gtgacggggg aggaactgtg attgcgacag ggacaccgga acaggtcgca
      901 aaggaaaaag catcctatac gggttattat atcgggaaaa tgctgaaaaa aagaaaatga
      961 tactaaagta tttttgtttt attccgataa tataattaga gtgcatggag gcgcgtaaaa
     1021 cggatggaac cgtcccgttt ttcgcgccct tttattattt tatgaaaaaa atataaaacc
     1081 tttgaaaatc aaatagcttt gaggtatact atagtcgtat gtttgtgttt attatttgaa
     1141 ttttttggta aaataaataa gaaaccatgc gaaaggggaa tatgattatg gtttataccg
     1201 atataggaat cgatcttggt acagcaagta tccttgttta tatccggggg aagggtgttg
     1261 tattgaagga gccttccgta gtcgcatttg acagggatac aaataagatc aaggcaatcg
     1321 gagaagacgc aagattgatg ctcggacgta cacccggtaa tattgtggct gtcaggccgt
     1381 tgagacaggg cgttatttcg gactataccg ttactgaaaa gatgctgaaa tatttcatac
     1441 agaaagcaat cggaaaaaag agctttagaa aaccgcggat cagtgtatgc gtaccaagcg
     1501 gcgttacgga agttgaaaag aaagcagtag aggatgcaac ttatcaggca ggagccaggg
     1561 aggttgcaat tatagaggaa ccgatcgctg cggcaatcgg tgcagggatc gatatcagca
     1621 agccatgcgg caatatgatc gtcgatatcg gcggcgggac gactgatatt gcagtgattt
     1681 ccttaggtgg aactgttgtc agtacatcaa tcaagatcgc cggggatgat ttcgacgagg
     1741 cgatcgtcag gtatatgaga aagaaacata acctgctgat cggtgagaga actgcagaag
     1801 atctgaaaat aaaaattggt gccgcattca aaagaccgga agtggattat ctggaagtaa
     1861 gaggccgtaa tcttgtaacc ggcctgccga gaaccgtaaa ggtgtcttcc gaagagacag
     1921 aggaagcgct tcgtgagacg acttcccaaa tcgtagaagc gatccacagc gtacttgagc
     1981 ggacaccgcc cgagcttgcg gcggatattg cggaccgggg tattgtattg acaggcggcg
     2041 gcagtctttt gcggggactg gaagatctga tcgcatataa gacgggaatc aatacgatga
     2101 cggctgagga tccgatgacg gcagtcgcta ttggtacagg taaatttgtg gagtttcttg
     2161 caggatattc ggatgatgtt gattaagtaa agagggatac gtatggtaaa aggattgtat
     2221 accgcgagca ccggaatgat aaatgaacag agaagagtcg atgtattgac aaataatatg
     2281 gcgaactctg cgacgaccgg ttttaaaaaa gagggcacga cttcggaagc gtttaaggat
     2341 gtgcttgctt ataagatcaa ggatacgtcg gaaccatggg gagcaaagac cctcggtaca
     2401 atgaatatgg gtgtaaagat cggagaaaat tatacggatt acagtcaggg agcgctgcat
     2461 gagacgggaa atacatttga cctggccttg tccggagatg gtttttttgc ggtagaattt
     2521 acagccaagg gtgaagacgg agaagcagga gaaacttctg tgaaatatac aagagacggg
     2581 tcctttacat taaacgccga cggggagctt gtaacaaaag acggcgacta tctgcttgat
     2641 gtggatgggg accatatcgt gcttgatcct acacaggaat tcagtgttga ccgtatgggc
     2701 aatattatgc aggatgatga aaatgtcggt cagattcaga taacggattt tgaagattat
     2761 gattatcttg aaaaatatgg agaaaattat ttccagactg tggacggagc tgcggaacag
     2821 gatgcggatg cagcggttta ttcgggctat ctggaaagct ccaatgtaca gatcgtgcag
     2881 gaaatggtcg atttaattac ttatacaagg gcttatgaga caaaccagaa aatgatacag
     2941 gcatttgatg aaacactcag taccgcagtc aatgacgtcg gtaaagttta aaaacctgtg
     3001 acaggaagtt cctgaaagga ggatgtttat ggtaagagca ttgtggacgg cagcatcagg
     3061 tatgatcgga cagcaggaca gcgtggatac gatcgcaaat aatctggcga atgtcaatac
     3121 aacagcttat aagacagaat cgaacgaatt taaatcgctg ctctatcaga cgatccagac
     3181 aaagacgaca acagcgaacg gagaaaataa accggtaggg gctcaggtcg gtcttggtgt
     3241 gcggactgct tcggtcacat ctcaatttgt ccaggggacc ttggtagact ctgaaagtga
     3301 tacggcattt gccattgacg gaaaaggatt ttttgccgtt gaaggggagg acggaaacac
     3361 ctattatacg agaaacggaa actttttatt ttcagtagga gtcaataata aaatgacgct
     3421 gtcgaattcc gacggacttc ctgttctgaa tacggcaggc aagccgatca cgattccggc
     3481 caattacgtt tccagcgagg ttgtcgtaga ttcggacggt cagctgtggt atcctgacga
     3541 cgatggtaca ctggaagaga tagcaggcat gcagattggg gtctttcagt ttcagaatcc
     3601 ttccggtctg gaaaagatgg gggatacatt atatgcggaa actgcagcat caggagccgc
     3661 actgaacgaa gcgacgaacg acaatctgag taagagtgag ctttatcagg gatatctgga
     3721 gaattcgaat gtagaggtcg cagatgagat ggtcaatctg atcattgcgc agcgcgctta
     3781 tgaaatgaat tccaaggcgg tgcagacagc ggatacgatg atgcaggaag caaacgagct
     3841 tagaagatag gggtgatgaa atggatatca gttcacttta cagcaattat ctggatacgg
     3901 tgtcacagac ttcatcgagt acaaacagtc tggaaaatac gttaaattcc gacatgtcgg
     3961 atgcgactga tgaggagttg atgagtgcct gcaaggagtt tg
//
LOCUS       DAOSYE010000076         3976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15740_length_3976_cov_4.035960, whole genome shotgun sequence.
ACCESSION   DAOSYE010000076 DAOSYE010000000
VERSION     DAOSYE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15740_length_3976_cov_4.035960"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLU43_02095"
     CDS             complement(<1..379)
                     /locus_tag="PLU43_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO27229.1"
                     /translation="MKRETVIVLDFGGQYNQLVARRVRECNVYCEIYSYKTDLSKIKE
                     MNPKGIILTGGPNSCYEPDSPTYSKELFELGIPILGLCYGAQLMMHVLGGKVARAKVR
                     EYGKTEVFLNNQTELFHGIEAPAI"
     gene            631..1974
                     /locus_tag="PLU43_02100"
     CDS             631..1974
                     /locus_tag="PLU43_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27230.1"
                     /translation="MEQKNLLFSKKALNKLIGPLVMEQILAVAVGMIDVIMIASVGEA
                     AVSGVSLVDNISILLIGLFSALATGGAVVAGHFLGQNDEENACRAANQIVLFVIVSSV
                     GIMILYLLGRNFILTVVFGKIEEDVMRAASTYLIITGLSIPGIALYNGCAALFRAMGN
                     SKITMWISLVMNVLNAAGNAVLIYLVHIGVAGAAIATTFSRYVAAVTIMILLFNKNNT
                     IHLDRHFEFRFHRAMIRKILYIGIPTGLENSIFQLGKILILSLVSVCGTYAIAANAVA
                     GTIGVLNVIPGNAMSLALLSVVSVCIGADNFEQARYYTKKLIIKVHIFTGIISAMIIL
                     FAPFIVKIYHLSEQTASATETLIRMHAVCAMLFWPESFVLPNTLRASNDVRFTMGISI
                     FSMWVFRIGASYLFVNVFDMNIVGVWMAMFIDWLFRAAVFVTRYIRGRWQSKYTA"
     gene            complement(1986..2333)
                     /locus_tag="PLU43_02105"
     CDS             complement(1986..2333)
                     /locus_tag="PLU43_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO27231.1"
                     /translation="MMNISKLAAAGVDYEDGLNRFCGKEELYQKYILRFPQQKEYHQL
                     LAAIGKKDCKEAFEYAHALKGITGNLSFIELRKILTELTEELKSGNLEAAGKYVPMLE
                     KEYQALVEIIEEQ"
     gene            2494..>3976
                     /locus_tag="PLU43_02110"
     CDS             2494..>3976
                     /locus_tag="PLU43_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO27232.1"
                     /translation="MAKRERLFLFISSIMILVSFFTAFTASAVHAGEQQKTVRIGYID
                     YEGFITKEENGTYSGYGVECLDTISDYTGWNYEYVYDSLENQIENLKTGKIDFFCHAQ
                     KTNEREKDFLFSKYSIGSESGVLYVRSDDTRYFYNDFPNFNNMKIAFIKNSFHNEEFA
                     DYAQNKGFQYTSVEFDTQSECFAALKNKTVDAVAKGSLALNPDFSVVCRYGAYPFYII
                     TNKSSRSLLDDLDDALGQIMAINPSFTSSLYDQYYGTINHSQEIIFTREEASFINTTP
                     EVTVCFLPDRAPFSYIDENGTPAGIMIDIVKLIEERSGLHFLLEMKPDGIRSLDYMEE
                     HPNALIAGIWEDAPEFQANTYLLSDVIYSDEIALACRSGYSYNFSAPDTTYKLAIPNC
                     FNAFKSSISKDYPEFKIVECSSDLDCAKEVLSGNADFMAQNVNVITSLLSDPHYEGLT
                     VLPTFFMDEDFGIVCKTNTDNKLLMSIFNKCIISITDKELSQFT"
BASE COUNT         1082 a          864 c          814 g         1216 t
ORIGIN      
        1 agatcgccgg agcttcgatg ccatgaaaaa gttcagtctg attgtttaga aatacttcgg
       61 tctttccgta ttcgcgtact tttgcgcgtg cgacttttcc gccgagcaca tgcatcatca
      121 gctgtgcacc atagcacagg ccaaggatcg gaatgccaag ttcaaataat tctttgctgt
      181 atgttgggga atccggctcg taacagctgt tcgggccgcc ggtcagaata atgcctttgg
      241 ggttcatttc cttgatttta gaaagatcgg tcttataaga ataaatttca cagtaaacat
      301 tgcattccct gacccttctt gccacgagct gattatactg cccgccaaaa tccagtacga
      361 tgactgtttc tctcttcatg tttcctcctc tgtgccgtat ctgctgccgt aaggctgcgg
      421 ccaattgatg tgaacgatat aagaattaca gggattttac agccttgtgc aaaatgaccc
      481 atgactaatt ataatgaact cacttaaaat atacaagatg ttttcataaa aagcaggttt
      541 tcactcttgc atatttctga atagctgtta gaataaatct tacacggatt ttacatttga
      601 attttatgtt ttgacgggca ggacaataaa atggaacaaa aaaatcttct tttttcaaaa
      661 aaagcgctga ataaattgat cgggccgctt gtaatggaac agattctggc tgtagcagtc
      721 ggtatgatcg atgttattat gatagcaagc gttggtgaag ctgcagtttc cggtgtttca
      781 cttgtcgaca atatcagcat tctgctgatt ggtctgttca gtgcactcgc aacaggcggt
      841 gcagttgttg ccggccattt tttaggtcag aacgacgaag aaaatgcctg cagggcagcc
      901 aaccaaatcg tattattcgt tattgtctct tctgtcggca ttatgatcct gtatctgctc
      961 ggcagaaatt ttattttaac agttgttttc ggaaagatcg aagaggacgt tatgcgtgcg
     1021 gcaagtacgt atttgatcat caccggactt tccattcctg gaatcgcact gtataacgga
     1081 tgtgccgcac tgttccgtgc aatgggaaat tcaaagatca ccatgtggat ctcgcttgtc
     1141 atgaatgtac tcaatgctgc cggaaatgct gttttgatct atctcgtaca tattggggtt
     1201 gcaggcgcag ccattgccac aacattttca agatatgtgg cagctgtcac gatcatgata
     1261 ctgctgttca ataaaaacaa tacgatccat cttgacaggc attttgaatt ccggtttcat
     1321 cgggcaatga tcagaaaaat tctgtatatt gggatcccga ccggactgga aaacagtatt
     1381 tttcagcttg gaaagatttt gatcttaagt ctggtgtccg tctgcggcac gtatgcgatc
     1441 gctgccaatg ccgtcgcagg aacgatcggc gttctgaatg ttattcccgg aaacgccatg
     1501 tcacttgccc tgctttctgt tgtatcggtc tgcatcgggg ccgacaattt tgaacaggca
     1561 cgctattata caaaaaaact gatcatcaaa gtccatattt ttaccggcat catcagcgct
     1621 atgatcattc tctttgcgcc gttcatcgta aagatctatc atttatcgga acagactgct
     1681 tccgccacgg aaaccctgat ccgcatgcat gctgtctgtg ccatgctgtt ctggccggaa
     1741 tcattcgtac tgccaaatac actgcgcgca tcgaatgacg tcagattcac aatgggaatt
     1801 tccatttttt ccatgtgggt attccggatc ggtgccagct atctgtttgt gaatgtcttt
     1861 gatatgaata tcgtcggtgt ctggatggca atgttcattg actggctttt cagggcagct
     1921 gtctttgtta cccgttatat ccgcggcaga tggcagtcca aatatacggc atgatctgat
     1981 gcgccttatt gttcttcaat gatctctacc agtgcctgat attctttttc cagcataggc
     2041 acatattttc cggccgcttc cagattgccg cttttcaatt cttctgtcag ctcggtaagg
     2101 atctttctca attctataaa gcttagattt cccgtaattc cttttaatgc atgtgcatat
     2161 tcaaaggctt ccttacagtc ttttttaccg attgccgcca aaagctgatg atattccttc
     2221 tgttgtggaa agcgaaggat atatttctga taaagctctt cttttccgca aaaccgattt
     2281 aacccatcct cataatcaac gcctgctgcc gctaatttcg aaatattcat catatcgctc
     2341 ccttctgaac taattatcaa ctgagttcta gtaaaatagt atccttgatt acataaaatg
     2401 tcaaataaaa tcaaaaaaat gacatttttt cttgagattt aaaaatgtat gttctatgat
     2461 aaaaatagac ggaacaaaga gaggaggctt cacatggcaa aacgggaacg actttttctt
     2521 ttcatttctt caattatgat cctggtttct tttttcaccg catttacagc atctgctgtt
     2581 catgccggag aacagcaaaa aactgtcagg atcggttata tcgattacga gggctttatt
     2641 acaaaagagg aaaacgggac ttattccgga tacggtgtcg aatgtctgga tacgatctcg
     2701 gattacaccg gctggaacta tgagtatgtt tacgattctc tggaaaatca gatcgaaaat
     2761 ttaaaaaccg gaaaaattga ttttttctgt catgcccaga aaacaaatga acgggaaaag
     2821 gattttcttt tttccaaata ttcaattgga tcggaatccg gcgtcctcta tgtccgcagt
     2881 gacgataccc gttattttta taatgatttc cccaatttta ataatatgaa gatcgccttt
     2941 atcaaaaaca gcttccataa cgaagaattt gccgattatg cccaaaataa aggctttcaa
     3001 tatacttctg tggagtttga cacacaaagt gagtgttttg cggccctgaa aaacaaaacc
     3061 gtcgatgctg tagccaaagg cagtctcgct ttgaatcctg acttcagcgt tgtctgcaga
     3121 tatggtgcgt atccgttcta tattattaca aacaaaagca gccggtccct tctggatgat
     3181 ctggacgatg cgctcggtca gatcatggcg atcaatccct cgtttacatc cagcctgtac
     3241 gatcaatatt atggaacgat caatcatagc caggaaatca tctttacacg ggaagaagct
     3301 tcctttatca atacaacgcc cgaagtaact gtctgctttc tgccggatcg tgcacctttc
     3361 tcctatattg acgaaaacgg aacacctgcc ggtattatga tcgatattgt caaactgatc
     3421 gaagaacgaa gcggtcttca tttcctgctc gaaatgaagc cggacggcat acggtcactg
     3481 gactatatgg aggaacatcc aaacgcactg attgcaggca tttgggaaga cgctccggaa
     3541 tttcaggcta atacatactt actgagtgat gtcatttatt ccgatgagat cgcattggcc
     3601 tgccgatcag gatactccta caatttcagc gcaccggata ccacttataa gctggcgatc
     3661 cccaattgct ttaatgcatt caaatcttct atttccaaag attatccgga atttaaaatt
     3721 gtggaatgca gctctgattt ggactgtgct aaagaagttt tatccggaaa tgctgatttc
     3781 atggcacaga atgtcaatgt catcacatcc ctgctctctg atcctcacta tgaaggtctg
     3841 actgttctgc cgaccttttt tatggatgaa gattttggga tcgtctgcaa aacaaatacg
     3901 gataataaac tgcttatgag cattttcaac aaatgcatta tcagcatcac agataaagaa
     3961 ttatcacaat ttacag
//
LOCUS       DAOSYE010000077         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15926_length_3947_cov_3.670606, whole genome shotgun sequence.
ACCESSION   DAOSYE010000077 DAOSYE010000000
VERSION     DAOSYE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15926_length_3947_cov_3.670606"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLU43_02115"
     CDS             complement(<1..217)
                     /locus_tag="PLU43_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27233.1"
                     /translation="MKTVKKIAGLLLGIFITLSFMTRIAYADTDPGEYQYKVLYISSY
                     SENFITNPDELEGIRSVFDPVGIDLDTE"
     gene            complement(404..2569)
                     /locus_tag="PLU43_02120"
     CDS             complement(404..2569)
                     /locus_tag="PLU43_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOO27234.1"
                     /translation="MKKSMDLMLFPTLRHYKKSYLRGDLQAGIIVLFMSIPISMGYAQ
                     ITGLPAVYGLYGSVLPILFFAMFSTSFQIIFGIDAAPAALVGSFLASQGIEAASKEAL
                     AAVPKIAFLTAVWLFLFSIFKAGRLIKYISGSVIGGFVSGIACSIILMQIPKLMGGTA
                     GTGEFLQIAVHLISSFEKIQLPSFLLGMVSLCIIFLLRKWAPKVPGPVVIMILGALLQ
                     FLFHLDRYGIALLPKVVQELPAFFLPSFSFKTILGNLGISLTVAVVILSETLLTSNDL
                     ALKNKYTIRPGREIFAYAIGNLISALTGNCPVNGSISRTKMSEKYKGKTQAVSLTAGI
                     GMIFVLLFGTKIIGYLPVPVLTAIIIFALYNVLDIDLAVRLYKLDKGELFIFLSAFVG
                     VVLLGTIYGVLLGVALSFISILRKETVPHREFLGVIPGYSGFFSLKRNRNSRPISQVV
                     IYRFNSNLFFANVSVFAEDIEASIKADTKVVIVDASAITDIDLAAVDRLRLLYNSLKQ
                     RGIRFYLTEHIGELNDKLKKIGCHQMMRDGVFRRTIKIALADAGFVRPFVLEEGGEDS
                     REQTDLHKSIISEFEWAFGDQADQEMENYISRYLQDTGKKMETVQQPEKAVGGIKLWH
                     NIGKVEEDDILEHLELHISELAEKFSIKEEVLWKEISRRRENIETNLKAENPHAALML
                     KKHRRELDALMQRRHRQIYDQMIQRRKQYKTDRKKGIKH"
     gene            complement(2655..2786)
                     /locus_tag="PLU43_02125"
     CDS             complement(2655..2786)
                     /locus_tag="PLU43_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27235.1"
                     /translation="MILPKFLGIDGVLWGGPAADALAFVLALAVTIAEVRRLKAIDQ"
     gene            complement(2758..3177)
                     /locus_tag="PLU43_02130"
     CDS             complement(2758..3177)
                     /locus_tag="PLU43_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27236.1"
                     /translation="MTEENVLVKESAFGTEPVGKLISKFAIPCVISLVVNGLYNIVDQ
                     IFIFIVVGIVTGAQPIIGFNRGAGNFERVMKAFKICVISAAVASIAALYSERIPDGSK
                     YFCAGDRASAAGYDTFFVTPDPVPDSGGDDPAEIPWY"
     gene            3375..3788
                     /locus_tag="PLU43_02135"
     CDS             3375..3788
                     /locus_tag="PLU43_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO27237.1"
                     /translation="MHELMSRFSIIHRQSSIYLDHRLKEHHITGSQYTHILVICENPG
                     ISQEEISDKLKIDKGSVARMIKQFASEHYITRLISKEDKRQYQIYPTDKAKAVYEKIH
                     GTAVASENRLTKGFTDVERDILINLLDKIITNLEE"
BASE COUNT         1174 a          958 c          715 g         1100 t
ORIGIN      
        1 attccgtgtc aagatctatc ccaaccggat caaaaacact tctgatccct tccagctcat
       61 ccggatttgt tataaaattt tcactgtagg aactgatata taatactttg tattgatact
      121 caccgggatc cgtatctgca taagcgattc ttgtcataaa tgataaagtt ataaaaatgc
      181 ccagcagcaa acccgcaatt ttttttactg ttttcattta ttcttcctca ctgaccggcc
      241 aaactgttac aaatgtctgt tttcctgcat ccgacaagct gaaatagaag tcttatgatt
      301 ttacacctta cataatttta ttgtatcaaa atttatcaga caacgcaagg tcttgccatt
      361 catttttctg acaatttcat gaaattattt cataaaacat actttaatgt tttattcctt
      421 tttttctgtc tgttttatat tgttttctgc gctgtatcat ctgatcgtag atctgtctgt
      481 gccttctttg catcagggca tccagctctc tcctgtgttt tttcagcata agcgctgcat
      541 gcggattttc tgccttcaaa tttgtttcta tattttcacg ccttctggaa atttccttcc
      601 agagtacttc ttcctttata ctaaatttct cagccagttc ggaaatatga agttccagat
      661 gttccagaat atcatcctcc tccacttttc cgatattgtg ccaaagcttt attcctccga
      721 cagctttttc cggctgctgg acagtttcca ttttctttcc ggtatcctgc agataacgac
      781 tgatataatt ttccatctcc tgatctgcct gatccccaaa cgcccattca aattcgctga
      841 ttatgctttt atgcagatct gtctgctccc tgctgtcctc tccgccttcc tccaatacaa
      901 acggacgtac aaagccggca tctgccagtg caattttgat cgtacggcgg aatacgccgt
      961 ccctcatcat ctgatgacag cctattttct ttaacttatc attcaattct ccaatatgtt
     1021 ccgtaagata gaagcggatc cctctctgtt tcagactgtt ataaagcagc ctgagcctgt
     1081 caactgccgc aaggtcgata tccgttatag cgctggcatc gacgatcacg acttttgtat
     1141 cggcctttat acttgcttcg atatcctcag caaaaacaga aacattggca aaaaataaat
     1201 tactgttgaa tcggtaaata accacctgtg agatcggcct ggaatttcgg tttcttttca
     1261 ggctgaaaaa tccgctgtaa ccaggtataa cacccaaaaa ttccctgtgc ggcactgttt
     1321 ctttccgcag aatggaaata aacgaaagcg caaccccaag gagcactccg tagatcgtac
     1381 ctaacaggac gactccgaca aacgcggaga gaaaaatgaa cagctccccc ttgtccagct
     1441 tatacagacg cactgccaga tcgatatcca aaacattata tagggcaaat ataataatcg
     1501 cagtcagtac cggcaccggc agatacccta tgatttttgt gccaaacagc agtacaaaga
     1561 tcataccaat gccggctgtc agcgagaccg cctgcgtttt tcccttatac ttttccgaca
     1621 tcttcgttct ggatatgctg ccgttgaccg ggcagttccc agtcagggcg gaaatcagat
     1681 tgcctatggc atacgcaaaa atttcccttc ccggccggat cgtatactta tttttcaatg
     1741 ccagatcgtt ggatgtcagc aaagtctctg ataaaatgac gaccgcgacc gtcaggctga
     1801 tgccaagatt gcctaagatc gttttaaaag aaaacgacgg cagaaaaaaa gcaggcagct
     1861 cctgaactac cttgggcagc agtgcgatcc cataacggtc caaatgaaaa aggaactgta
     1921 aaagagcacc taaaatcata attactacag gacccggcac ctttggcgcc cattttctta
     1981 ataaaaaaat aatacacaat gaaaccattc ccaaaagaaa cgacggcagc tgtatttttt
     2041 caaacgaact gatcagatgg acggcaatct gcaaaaattc ccctgtaccg gccgttcctc
     2101 ccatcagctt cggaatctgc ataagaatga tactgcacgc gattccgctt acaaatccgc
     2161 cgattacaga gcctgaaata tatttgatca gtctgcctgc cttgaaaatg ctgaataaaa
     2221 acagccatac agctgtcaga aaggcaattt tcggaactgc cgcaagcgct tccttagaag
     2281 ccgcctcaat tccctgactc gccagaaaac tgcctaccaa agcagcaggt gcggcgtcta
     2341 taccgaaaat aatctgaaaa gatgtggaaa acatggcaaa aaacaggatc ggaaggaccg
     2401 atccgtataa gccgtacacc gcagggagtc cggtgatctg cgcatagccc atggagatcg
     2461 gaatcgacat gaacaatacg atgatacctg cctgcaggtc accccgcaga tatgattttt
     2521 tataatgccg tagtgttgga aagagcatga gatccatact ttttttcata actgtccgtt
     2581 acctccaact aaaagcgtat ctctatatta ccattctctc cttcacttgt atagaaaaaa
     2641 taacggcaaa atttttattg atcgatcgcc tttaacctcc tgacttccgc aatcgtaaca
     2701 gcaagcgcca gtacaaaggc cagcgcatcc gctgccggac cgccccataa aactccgtca
     2761 ataccaagga atttcggcag gatcatcacc gccggaatca ggaacaggat ctggcgtgac
     2821 aaagaaagta tcatagcctg cagcggacgc tcgatcgcct gcacaaaaat acttgctacc
     2881 gtctggaatc cgttcagaat acaaagcagc aatcgaagca accgcggcgc tgatcacaca
     2941 gatcttaaat gctttcatca cacgctcaaa attccctgcc ccgcggttaa aaccgatgat
     3001 cggctgtgca ccggtcacaa taccgacaac gataaaaata aagatctgat caacaatgtt
     3061 atacaatccg tttacaacaa gcgagatcac acaggggatc gcaaacttgg aaatcagctt
     3121 tcctaccggt tctgtcccga atgcgctttc ttttaccaat acattttctt ctgtcataat
     3181 atcctcctat catttagttg cctaagcaac ttgtttttta aaaaaagatg ctttttaatt
     3241 gccaaagcaa ctataattct acgcatgatt catgaaatag tcaagtaaaa caattcattt
     3301 tatttgacag tctttctatt ttcttgtatt atttagttgt ctaggcaacg ttaaatcata
     3361 aagggggtat ccgcatgcac gagcttatga gccgattttc catcatccac cgacagtctt
     3421 ccatatatct ggatcatcgg ttaaaggaac atcacatcac aggcagccaa tacacacata
     3481 ttttagtcat ctgtgaaaac ccaggcattt cgcaggaaga aatctcagat aaactgaaga
     3541 tcgataaagg ctctgtggca agaatgatca aacagttcgc atcggaacat tatattacaa
     3601 gactgatctc aaaagaagat aagcggcaat atcagatcta tccgaccgac aaagccaaag
     3661 ctgtttacga aaagatacac ggtactgccg tagccagtga aaaccggctg acaaaaggat
     3721 ttaccgatgt ggaacgggat atcctgatca atctgctgga caagatcatt acaaacctgg
     3781 aagaataatt gtctttttca acaaaaaaag ctgacaattt tatagaataa ttgtcagctt
     3841 tttttattga cattctgatt tatttttcct ataatgtttt atgtcgaaca gttccatata
     3901 caacattatg aaaaggagat tacaatgtcg gaaaaaagcg ttggtct
//
LOCUS       DAOSYE010000078         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_15929_length_3947_cov_2.945786, whole genome shotgun sequence.
ACCESSION   DAOSYE010000078 DAOSYE010000000
VERSION     DAOSYE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15929_length_3947_cov_2.945786"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLU43_02140"
     CDS             <1..234
                     /locus_tag="PLU43_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27238.1"
                     /translation="FVRWNTVWAILGSYLFGLCYWAYAFVPSITGISVNTDIAQILPY
                     VVTIIVLIAGNMRKNKSHPGPAYLGQSYFREER"
     gene            477..1046
                     /locus_tag="PLU43_02145"
     CDS             477..1046
                     /locus_tag="PLU43_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27239.1"
                     /translation="MPPREKVSKEQVLNTAFEMTREHGFEELTARKLAEKLGSSTQPI
                     FRVYENMEMLKKDLFFKSAEFFSDYMLKKCNKSTPVYLSMGMAYIELAQSESHLFKLI
                     AAIEDYGTDAIKEFLQQGDCMELLEKLPDTKTLTEKKKKELFLMVWMFTHGIATMVTS
                     KRVTLSDKEIKKMLTKAYEGFVAVQKEDG"
     gene            1086..1397
                     /locus_tag="PLU43_02150"
     CDS             1086..1397
                     /locus_tag="PLU43_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27240.1"
                     /translation="MTNAQRKRVRMKKRLKRNVKRTIRMILLAPGKLPRVRLHMLEQA
                     VRVCEISGIIFVVSILTYLLQPDNINIALSIVSALIFMGFLFVIKLEQYQEVLDRQEY
                     L"
     gene            1413..1541
                     /locus_tag="PLU43_02155"
     CDS             1413..1541
                     /locus_tag="PLU43_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27241.1"
                     /translation="MYSAEGALSIDKDASAGGQGKCAGEYFIYDYIAAAFWETSIM"
     gene            complement(1571..2140)
                     /locus_tag="PLU43_02160"
     CDS             complement(1571..2140)
                     /locus_tag="PLU43_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOO27242.1"
                     /translation="MDNKRDVTKILLVNSFKKMVLTKPFEKITIKMITDDAGVIRPTF
                     YNYFQDKYEVLEWIFTHEIADKMNLLLENKMESEAIKMLFICLEKEREFYKRAFLITG
                     PNSFYQLLEDFTYKTFLHLLAEYPLKPEARIHFLTSESIATYYTFSICEAVRAWVIND
                     GFHMTSDEIYEAYIYLIHHSLLDTIDMHK"
     gene            2438..3016
                     /locus_tag="PLU43_02165"
     CDS             2438..3016
                     /locus_tag="PLU43_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOO27243.1"
                     /translation="MHVIEIVLIVIGLSLDVFAYALYKGAMLSKIDKGNVAKCCLIFS
                     VWQVGGLLLGNLITLIPFFTASHEAAKHLWRICAAVIFLAIGLYMIIKGAKKEEIIER
                     KENQFRFGQLAVWACITSIDAVFAGIGFAFLDTQLLVTAFVTALTTIAVVIVGILCGY
                     RLGCGVKNRAIAVGGCILLVAAFDIAIRYISI"
     gene            3116..>3947
                     /locus_tag="PLU43_02170"
     CDS             3116..>3947
                     /locus_tag="PLU43_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO27244.1"
                     /translation="MGDIGHKVKRAAFEGVLEKVLKDVKKDREATLLKLIDMTEDLMK
                     DHIDTAIYPNARKLVKDPDSKWMKYFDTLVDEIDPYVLKMTLMNLGFESMLAGTKQMH
                     KMREKYNCNIPWVILMDPTSACNLHCTGCWAAEYGHTLSLSYEELDRIVTQGKELGVH
                     FYMYTGGEPLVRRADIMKICKKHNDCMFLAYTNGTLVDEEYCKEMQQAGNLALAISLE
                     GYEEVNDGRRGAGVFDKVMHAMDLLKQYGLVFGTSICYTRNNIETVTSDEFLDMIIAK
                     G"
BASE COUNT         1227 a          672 c          991 g         1057 t
ORIGIN      
        1 tttgttcgct ggaatacggt gtgggcaatt cttggttcct atctgttcgg tctttgctac
       61 tgggcgtatg cgtttgttcc gagtataacc ggaatcagtg taaatacgga tattgcacag
      121 atcctgccgt atgtcgtaac gatcattgtg ctgatcgcgg ggaacatgag gaaaaataag
      181 agccatccgg gaccggcgta tcttgggcag tcctatttca gggaagaacg gtaaaaaatc
      241 attgataaaa gtatagagcg gaaaagtccg gggatgggtc tccggacttt tttgcgttag
      301 ggaaaaggct atggaaagtt aacataaatt ataaaaagtg gttgacatag tttccataat
      361 atagtaagat aacattgtta tataacttcg atgtgtaact aagatacgta acatagatac
      421 ataacgtgga tatgtaactt tgatacgtaa caaagttata agacagggag attgaaatgc
      481 ctccacgaga gaaggtttcg aaggaacagg tgctgaacac agcatttgaa atgacaagag
      541 aacatggttt cgaagaactg acagcgagga agctggcgga aaaactgggt agttctacac
      601 agcctatttt tcgtgtttac gaaaacatgg aaatgctgaa aaaagacctg ttttttaaga
      661 gtgctgagtt tttttccgat tatatgctga aaaaatgcaa taaaagcaca cctgtttatc
      721 tttctatggg aatggcctat atcgaactgg cacagagtga gagccatttg ttcaagctga
      781 ttgctgcgat cgaggattat gggaccgatg caataaaaga atttctgcag cagggagact
      841 gtatggaact tcttgagaaa ctgcctgata caaaaacact gactgagaag aaaaagaagg
      901 aattgttttt gatggtctgg atgtttacac acggcattgc aacaatggta acaagcaaac
      961 gggttacact atcggataag gaaataaaaa aaatgctgac aaaagcctat gaagggtttg
     1021 tggctgttca aaaagaggac ggatagccgc atgggctgat gtgaaaatgc aatggaggga
     1081 ttgcagtgac aaacgcacag cgtaaacgcg taaggatgaa gaaacgctta aaaagaaatg
     1141 tgaagaggac gatcaggatg attttactgg cgccggggaa gctgccaaga gtcagacttc
     1201 acatgttgga acaggcagtc agggtctgtg agatcagcgg gatcatattt gtggtctcca
     1261 tattgactta tctgcttcag ccggacaata tcaatatcgc actcagtatc gtgtcggctt
     1321 tgatatttat gggatttctg tttgtgatca agctggaaca gtatcaggaa gttctggaca
     1381 gacaggagta tttgtaaaga aaatggatcg tattgtacag tgcagagggt gcactgtcga
     1441 tcgacaagga cgccagcgca ggcggacagg gaaaatgcgc cggggaatat ttcatatatg
     1501 attacatagc tgccgcgttt tgggaaacgt caattatgta aaaagagcag atgcagggga
     1561 agcgtctgct ctatttatgc atatctatcg tatcaagcag ggaatggtgg atcaggtaaa
     1621 tatatgcttc atagatctca tcggaagtca tatggaaacc gtcatttatg acccatgcgc
     1681 ggacagcttc acaaatactg aaggtataat aagtcgcgat gctttccgaa gtcaggaaat
     1741 gaatacgggc ttccggtttc agcggatatt cggcaagcag atgcagaaaa gtcttataag
     1801 taaaatcctc cagcagctgg taaaagctgt tcggtccggt aattaaaaac gcacgtttat
     1861 aaaattcccg ttctttttcc aaacagataa acagcatttt gatcgcctca gattccattt
     1921 tattttctaa aagaagattc attttatcgg caatttcatg tgtaaaaatc cattccagaa
     1981 cttcatattt gtcctgaaaa taattataaa atgtcggccg gatcacaccg gcatcatccg
     2041 tgatcatctt gattgtgatt ttttcaaatg gctttgtcaa taccattttt ttaaagctgt
     2101 ttacgagcaa aatttttgtc acgtctcgtt tattatccat tacacgcctc caaagctgtc
     2161 ggtaaaagaa aacgtcacat gtctgaagaa gtttcttatc ttatgattag tttaacatga
     2221 aaattcctta aaaaacagta tttcataaga aaagacgaca tattttaaaa tctgtagccg
     2281 gatgtctgac tttttaaagc atatgttcat atttgaaaaa cagacggtcg gggctgtatc
     2341 ttacatgaga agaaaacaga gcctataata aagataacca aatgattcgg atgttctttg
     2401 aggatacgac ggaaaatcgg caggagagag tggttttatg catgtgatag aaattgtcct
     2461 gatagtcatc gggctatcgc tggatgtatt tgcctatgct ttatataagg gagcaatgct
     2521 ttccaagata gacaagggaa atgttgcaaa gtgctgtctg atattttcag tctggcaggt
     2581 gggcggactg ctgcttggaa atctgattac actgatcccg ttttttacag cgtcacatga
     2641 agcggcaaag catttgtggc ggatctgtgc ggcagtgatt tttcttgcga tcgggctgta
     2701 tatgatcatt aaaggggcaa aaaaggaaga gatcatagaa cgaaaagaaa accagttccg
     2761 gtttggtcag ctggccgtat gggcctgtat tacaagcatt gatgctgtat tcgcaggtat
     2821 cggatttgct tttcttgata cgcagctgct tgttacggcg tttgtaacag cactgacaac
     2881 gatcgcggta gtgatcgttg gcattctttg cggatatcgg cttggctgcg gggtcaaaaa
     2941 ccgggcgatc gcggtaggag gctgtatttt gcttgttgct gcatttgata tagcgatccg
     3001 ttatatttcc atctgataaa gcatgcaaat tcccatggga aacgaaaggg catgtgttca
     3061 tataaatgaa agaaaagaaa acagtaaaaa ccggcaggaa aacggaggga taaaaatggg
     3121 tgatatagga cacaaagtaa aaagagccgc atttgaaggc gtgctcgaga aagtattaaa
     3181 ggatgtaaaa aaggacaggg aagcaacact gctgaagctg attgacatga cagaggacct
     3241 gatgaaggat catatcgata cggcgatcta cccgaatgcg cgaaagcttg taaaagatcc
     3301 ggacagtaaa tggatgaagt attttgatac gctggtcgat gagatcgatc catatgtgct
     3361 gaaaatgaca ttgatgaatc tgggctttga atcaatgctt gcaggaacaa aacagatgca
     3421 taaaatgagg gagaaatata attgcaatat tccatgggta attctgatgg atccgacgag
     3481 cgcatgcaat ctgcactgta cgggctgctg ggcggcagag tatggtcaca cgctgtcgtt
     3541 aagctatgaa gagcttgatc gtatcgttac gcagggaaaa gagctgggtg ttcattttta
     3601 tatgtatacg ggcggggaac cgcttgtcag aagagcagat atcatgaaga tatgcaaaaa
     3661 gcataatgac tgtatgttcc tggcatatac gaacggtacg ctggtcgatg aggaatactg
     3721 caaagaaatg cagcaggccg gtaatctggc gcttgccatc agcttggaag gctacgaaga
     3781 ggtcaacgac ggacggcgtg gcgccggcgt atttgacaaa gtcatgcatg cgatggatct
     3841 gctgaaacag tatggtcttg tatttggtac atccatctgc tatacaagaa ataatattga
     3901 aacagtgaca tctgatgaat ttcttgatat gataatagct aagggct
//
LOCUS       DAOSYE010000079         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16002_length_3935_cov_2.896392, whole genome shotgun sequence.
ACCESSION   DAOSYE010000079 DAOSYE010000000
VERSION     DAOSYE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16002_length_3935_cov_2.896392"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLU43_02175"
     CDS             complement(<1..321)
                     /locus_tag="PLU43_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOO27245.1"
                     /translation="MAAEKRMDQVILGFLNHEPMTGYEIKKRIDTTLRFFWSGSFGSI
                     YPTLNALEKKGYVTKAALKEGRREKLVYTITDAGRDSLKEWMLLPALKDELHYETLLK
                     LFFGS"
     gene            complement(323..814)
                     /locus_tag="PLU43_02180"
     CDS             complement(323..814)
                     /locus_tag="PLU43_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOO27246.1"
                     /translation="MKDIVVYYSLEGNTKRAAERIAKELGADLMELKPVKDLSKEGGF
                     RYVKGGFQAMFGFCPKLKETKFDVNVYDRVILGTPVWAGKPAPAMNSFLKKNDIGKKA
                     AAVFTCSGSGDSASTAAHMKKCLPALCEELALIDQNSSKAGENEEKLEAFISRLRQVK
                     DDN"
     gene            complement(814..1551)
                     /locus_tag="PLU43_02185"
     CDS             complement(814..1551)
                     /locus_tag="PLU43_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO27247.1"
                     /translation="MKRQQIRKLLLLVSLLLFPVTMWYFSPAIISMAMLEHVMNGSFF
                     VFMAMLLLSPFFGRVFCGYLCPAGGLQECAAQISGRAAKQGKRDWIKFVIWSVWIVML
                     FLLFLLGKNKVTADFFYMTDHGISIAEIYNYVVYYGVILLIVLPALLHGRRASCHYIC
                     WMAPFMIIGKKIGQLLHVPLLHIEARKEKCIGCGKCSQVCPMGLDVVQMVQKEDGFFG
                     AECIVCGACADNCPKKVLYYSMKRRKR"
     gene            complement(1637..2161)
                     /locus_tag="PLU43_02190"
     CDS             complement(1637..2161)
                     /locus_tag="PLU43_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27248.1"
                     /translation="MKNDYKSTKFLLLFFGITLAWTWLCGFIPVMAGLTGTPAGTFLF
                     YFGGGAPSVTALFLVFLTYPVEVRRDYFHRCFSFRYMGWKWPLFTLLYIPAVILLNFA
                     VTFVYVKTKRSILAGALVHMLANLIGSQLLSPYSMEMSMCIRYVRMACCLAVAVYAMV
                     SHGFRDEVKALFDN"
     gene            complement(2292..3137)
                     /locus_tag="PLU43_02195"
     CDS             complement(2292..3137)
                     /locus_tag="PLU43_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27249.1"
                     /translation="MKTYDECYFLNYDNYDDILLFEVGCQKCLPHYDFGPIIRENFVL
                     HYIIAGCGTLYLSEKAFPVSAGEAFITPPNVLTYYQADAEHPWNYMWLHFNGKKVIEF
                     IRMLGITDTSPIFRSKYSSQKIEACITEILRNHEKELFCIGSMYCLFQQMQEQTELAA
                     EKKDYDSQLLYIRKAIEFIQRKYFEPIRITNIANYCGLNRSYLTKIFKEATGYSPQEY
                     LINYRIIKAKNLLRKHDIPVSYVAHSVGYTDPLAFSKLFKHKVGIAPCDYYEEYLAHK
                     DEVQI"
     gene            complement(3184..>3935)
                     /locus_tag="PLU43_02200"
     CDS             complement(3184..>3935)
                     /locus_tag="PLU43_02200"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO27250.1"
                     /translation="KIFHKYVLGVYSLYERLLEKFPEILFESCSSGGARFDPGMLYYA
                     PQTWTSDDTDAVERLKIQYGTSYVYPISSMGAHVSKSPNLQVGRMTPVETRGNVAMFG
                     TFGYEMDLDLLSENEKEVIVRQVAFVKKHRKLIANGLFYRLKSPFDKNVASWIIVSDD
                     QTEALAAYYQILKIPNGPFRRLSLAGLKEDRKYCVNGDKEHLYYGDELMYVGLMIDDA
                     YLCDNGGDFCSAVFEIEQVWEEKAAGKTVFN"
BASE COUNT         1128 a         1015 c          677 g         1115 t
ORIGIN      
        1 gcttccaaaa aaaagcttca gaagcgtctc ataatgcagc tcatccttta aggccggaag
       61 aagcatccac tcctttaagc tgtctcgtcc tgcatccgtg atcgtataaa cgagcttttc
      121 ccgccggcct tctttcaggg cagcttttgt tacataccct ttcttttcca aagcattcaa
      181 tgtcggataa atactgccga agcttccgct ccaaaaaaaa cggagcgttg tatcgatccg
      241 ttttttaatt tcatagcctg tcattggttc atgattcaaa aatcccaata tcacctgatc
      301 catgcgtttt tcagctgcca tttcagttat catccttcac ttgccgcaga cggctgatga
      361 acgcctccag cttttcttcg ttttccccgg ccttggaaga attctgatct atcagtgcaa
      421 gctcctcaca cagagccggc agacattttt tcatatgtgc cgcagtggaa gcagaatctc
      481 cgcttccact gcatgtaaaa acagccgctg cctttttacc aatgtcgttt ttctttaaaa
      541 aactgttcat ggccggcgcc ggttttcctg cccataccgg agttcctaaa atcacacgat
      601 catatacatt tacatcaaat ttggtctcct tcagttttgg acagaaacca aacattgcct
      661 gaaaaccgcc ttttacgtat ctgaacccgc cctcttttga caagtctttt accggcttca
      721 gttccataag atcggcccca agctcctttg caatccgttc cgccgctctt tttgtgttcc
      781 cctccaggga ataataaaca acaatatcct tcattaccgt ttcctccttt tcatggaata
      841 atataacacc tttttcggac aattatccgc acaggctccg catacgatac attctgcgcc
      901 gaaaaatcca tcctccttct gaaccatttg tacaacatcc agacccatcg ggcagacctg
      961 gctgcatttc ccacacccaa tgcacttttc tttcctggct tctatatgga gcagcggaac
     1021 atgcaacagc tgtccgatct ttttcccaat gatcataaac ggcgccatcc agcagatata
     1081 atggcaggat gcccgcctcc catgcagcag tgccggaagg actataagaa ggatcacccc
     1141 ataatagact acataattgt aaatttcagc aatagaaatt ccatgatccg tcatatagaa
     1201 aaaatctgcc gtcactttat ttttgccaag caggaacaat aaaaacagca tcacgatcca
     1261 gaccgaccat atcacaaact tgatccaatc cctctttccc tgttttgccg ccctgccgct
     1321 gatctgcgct gcgcactcct gcagccctcc tgccggacag agatatccgc agaaaaccct
     1381 gccgaaaaac ggcgacagca gcagcattgc cataaacaca aagaagctgc cgttcattac
     1441 atgctccagc atcgccattg aaataattgc cggggaaaaa taccacatcg taaccggaaa
     1501 cagcagcagt gagactaaaa gcagcaattt cctgatctgt tgtcttttca taacttaaaa
     1561 cctccgatcc tgtcatttta atatatcata ttgatatatc agttcgatat atattatatc
     1621 aaaaaaacaa agactgtcaa ttatcaaata atgccttcac ttcatcccgg aaaccgtgtg
     1681 acaccattgc atatacggcc actgccagac agcacgccat cctgacataa cggatgcaca
     1741 tagacatttc catcgaatag ggggaaagaa gctggcttcc gatcagattt gcaagcatat
     1801 gaacaagtgc acctgccagg atacttcttt ttgttttcac atacacgaat gtcactgcaa
     1861 aattaagcaa aataaccgcc ggaatataga gcagcgtaaa aagcggccat ttccatccca
     1921 tatatcgaaa gctgaagcat ctgtgaaaat aatccctgcg cacttccaca ggatacgtta
     1981 aaaatacaag aaacaacgcc gttaccgaag gggcgccgcc gccaaagtaa aataaaaatg
     2041 ttccggccgg tgtccctgta agtcctgcca ttaccggtat aaatccgcat aaccatgtcc
     2101 atgccagtgt aatgccaaaa aagagaagca gaaattttgt acttttataa tcatttttca
     2161 atcatagtat cctcatttca gttacttttc accgtttatt ttaatgcagt gccaatacaa
     2221 aaacaagaag cggatggcat aaaattacat ctgtcatccg cttctgttac atttattatg
     2281 atttctcaaa ctcagatctg tacttcatct ttatgtgcaa gatattcctc gtaatagtcg
     2341 catggcgcaa tcccgacctt gtgtttaaac agctttgaaa aagcaagcgg atccgtatat
     2401 ccgaccgaat gcgctacata gctgaccggt atatcatgct tccgaagcag atttttggct
     2461 ttgattatcc gataattgat caaatattcc tggggactgt aaccggtcgc ttccttaaat
     2521 attttcgtca gatagcttct gttcagtcca cagtaattgg ctatattcgt gatcctgatc
     2581 ggctcaaaat attttctctg gataaactct attgcctttc tgatataaag aagctgtgaa
     2641 tcataatcct ttttctccgc agcgagctct gtctgttcct gcatttgctg aaaaagacaa
     2701 tacatgcttc cgatgcagaa cagttctttt tcgtgattgc gaaggatttc cgtaatacat
     2761 gcttctattt tctgactgct gtattttgag cgaaagatcg gcgaagtatc cgtaattccc
     2821 agcatccgga taaactcgat aactttttta ccattaaagt gaagccacat ataattccac
     2881 ggatgttctg catctgcctg atagtaagtc agcacattcg gcggcgtgat aaaagcttct
     2941 cctgcagata caggaaaagc tttttcactt aaatacagcg taccacagcc ggcaataata
     3001 taatgcagta caaaattttc ccggatgatc ggtccgaaat cataatgcgg cagacatttc
     3061 tgacagccta cttcaaaaag cagaatatca tcataattat cataattcag aaagtagcat
     3121 tcatcatatg ttttcataaa ataccccttt tgctgattgt atcataaata cccgatcaat
     3181 tcatcaatta aaaacggttt tccctgctgc tttctcctcc catacctgct cgatttcaaa
     3241 cactgccgaa caaaagtctc ctccattgtc acatagatat gcatcgtcta tcataagacc
     3301 aacatacatt aattcatcgc cgtaatacaa atgttctttg tccccgttga cacaatattt
     3361 ccgatcttct ttaagccctg caagagacag gcgtctgaag ggaccattcg gaattttcag
     3421 tatctgatag tatgctgcca atgcctctgt ctgatcatca gaaacaatga tccacgacgc
     3481 cacatttttg tcgaacggcg atttgagccg ataaaacaaa ccgtttgcta ttaattttct
     3541 atgctttttt acaaaagcga cctgcctgac aatgacttcc ttttcattct ctgaaagcag
     3601 gtccagatcc atctcatagc cgaatgtccc aaacattgcg acgtttcccc ttgtttcaac
     3661 aggagtcatg cggcccacct gcagattcgg acttttggac acatgtgccc ccatactgct
     3721 gatcggatac acataagacg ttccatactg gatcttcagc cgttccacag catccgtatc
     3781 atcacttgtc catgtctgcg gtgcataata cagcatcccc ggatcaaaac gtgcaccgcc
     3841 gctggaacag gactcgaaca atatttcagg aaatttttca agcagacgtt cataaagact
     3901 gtacacccca agtacatatt tatgaaatat ttttc
//
LOCUS       DAOSYE010000080         3931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16026_length_3931_cov_3.597523, whole genome shotgun sequence.
ACCESSION   DAOSYE010000080 DAOSYE010000000
VERSION     DAOSYE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16026_length_3931_cov_3.597523"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PLU43_02205"
     CDS             <1..1043
                     /locus_tag="PLU43_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO27251.1"
                     /translation="SNASKELFEFAHRIEAPVCNTLMGKGGYDGLDELYTGMIGMHGT
                     KTANFGVSQCDLLIAVGARFSDRVTGNAKKFAKNAKILHIDIDPAEINKNIKTEASIV
                     GDIKEVLTLLNEKLPQQKHAEWITQITEYKEKYPLKYPKKELTCPYIMEEIYRITNGD
                     AIITTDVGQHQMWAAQFYKYTKPRTFLSSGGLGTMGYGLGACIGAKVGCPDKTVINIG
                     GDGCFRMNMNELATASRYDIPIIQVVINNAVLGMVRQWQTLFYEKRYSNTILKDKVDY
                     VKLAEALGCEAMRVTKKEEVAPAFEKAIAMNKPVVIECVIDSDDKVFPMVPAGKAIDE
                     AFDADDLKNIID"
     gene            1082..2476
                     /locus_tag="PLU43_02210"
     CDS             1082..2476
                     /locus_tag="PLU43_02210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOO27252.1"
                     /translation="MKIKVLRISGIVVSCVVAAACIVYLIIAFYYQNKFLYGTYINGV
                     SVKGMDTACVNDYLAQAYSDYMLVLDEGNEKEETISGSAVGFSADFTNELNEILNKQN
                     ILYWGYYFLYPVSYHIEPVYAYDEMKLNTVMKELDCFYLDTANRDATLEIKKEYLGYR
                     LIDETTNVMDTETATGLIKDALDRGLASVSLADCYVPYTPTEDMQEVYALWKKIDKVQ
                     QSTIVFEDGDLERTLNVGVIGDWIVADQNDMPILDENGQIQLDPEKVAAFAGELAALF
                     DTYGKDRLWHKKNGGTVWLQYSGSGYEVDQEAEIEKLTEDVLAGKTETRKPIYEREGS
                     GRGKDEIGNTYIEIDMSAQKLYYFVDGRIKLTTDVVTGNTSRGHGTPAKICAIYAKQK
                     NRVLRGPNYAAFVYYWMPVSGNIGIHDATWRDEFGGEIYKTDGSHGCINLPKEKAAKL
                     YEMAEIGTPVILYY"
     gene            2519..3610
                     /gene="serC"
                     /locus_tag="PLU43_02215"
     CDS             2519..3610
                     /gene="serC"
                     /locus_tag="PLU43_02215"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOO27253.1"
                     /translation="MKRVYNFSAGPAVLPEEVLKEAADEILDYKGCGMSVMEMSHRSK
                     VFEQIINEAEADLRELMSIPDNYKVLFLQGGASQQFAMIPMNLMKNGSADYIVTGQWA
                     KKACKEASAYGKATIVASSEDKNFSYIPDCSDLPISEDADYVYICENNTIYGTKFKEL
                     PNTKGKPLVADVSSCFLSEPVDVKKYGIIYGGVQKNIGPAGVVIVIIREDLITEDTLK
                     GTPTMLKFKTHADAASLYNTPPAYGIYICGKVFKWLLKMGGLTAMKERNEKKAKLLYD
                     FLDQSKLFIGTVEKDSRSLMNVPFVTGDQELDQKFVKEAKEAGFENLKGHRSVGGMRA
                     SIYNAMPLEGVAKLVEFMRKFEEETIKNV"
     gene            3603..>3931
                     /locus_tag="PLU43_02220"
     CDS             3603..>3931
                     /locus_tag="PLU43_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOO27254.1"
                     /translation="MFKYACLNPIAPIGLDQFTVDYEKCDDITSADCLLVRSASMHDK
                     ELPQTLLAVARAGAGVNNIPLDRCAENGIVVFNTPGANANGVKELVIAGMLLASRDIV
                     GGINWVK"
BASE COUNT         1206 a          710 c         1017 g          998 t
ORIGIN      
        1 tttcaaatgc gtcaaaggag ctttttgaat ttgcacacag gatcgaggcc cctgtctgta
       61 atacgctgat gggaaaaggc ggttatgacg ggctggatga attgtatacc ggcatgatcg
      121 gtatgcatgg cacaaaaaca gcgaactttg gtgtcagtca gtgtgatctg ctgatcgctg
      181 tcggggccag attttccgac cgtgtaacag ggaatgcaaa gaagtttgca aagaacgcca
      241 aaattctgca tattgatatc gatcctgctg aaatcaataa aaacattaaa acggaagcca
      301 gcattgtcgg ggatataaaa gaagtactga cgctgctgaa tgaaaagctg ccgcagcaga
      361 aacatgcgga atggatcacg cagattacgg aatataaaga aaaatatccg ctgaaatatc
      421 cgaaaaaaga gctgacctgc ccctatatca tggaagaaat ctatcggatc acaaatggtg
      481 atgcgatcat tacgacagat gtggggcagc atcagatgtg ggcagcacaa ttttataaat
      541 atacaaagcc ccggacattt ttgtcttccg gcgggcttgg gacaatggga tatggccttg
      601 gcgcctgtat tggtgcaaaa gtcggctgtc cggataaaac agtgatcaat atcggcggag
      661 acggatgttt ccgtatgaat atgaatgaac tggcgacggc aagccggtat gatattccga
      721 tcattcaggt cgtcatcaat aatgcggttc ttggcatggt gcggcagtgg cagactcttt
      781 tttatgaaaa gcgttattcc aatacgatac tgaaggataa ggtcgattat gtgaagcttg
      841 ccgaggcact tggctgtgaa gccatgcgtg ttacaaaaaa ggaagaggtt gcccctgcgt
      901 ttgagaaggc aattgcaatg aacaaaccgg ttgtgataga atgtgtaatt gacagtgacg
      961 ataaggtgtt tccgatggtt cccgcaggaa aagccattga tgaagctttt gacgcggatg
     1021 acctgaaaaa tattatcgat tgacaatatc cccattatgc tgacttgaag gaattacgag
     1081 gatgaaaata aaggttctga gaatttcagg catcgtagtg tcatgtgtag ttgcagctgc
     1141 ctgtatcgtc tatctgataa ttgcattcta ttatcaaaac aaatttttat atggtactta
     1201 tatcaacggc gtatctgtca agggaatgga tacggcctgt gtcaatgatt atctggcaca
     1261 ggcctattcg gactacatgc ttgttttgga tgagggcaat gaaaaagaag agacgatcag
     1321 cggaagcgcc gttgggtttt cagcagattt tacgaatgaa ttaaatgaga ttttgaataa
     1381 acagaatatt ttatattggg gatattattt tttatatcca gtttcctatc acatagaacc
     1441 ggtatatgcc tatgatgaaa tgaaattgaa tactgtcatg aaagaactgg actgctttta
     1501 tctggacacg gcaaatcggg atgcaacact tgaaatcaaa aaagagtatc ttggttatcg
     1561 actgattgat gaaacaacaa atgtcatgga tacggaaact gccacagggc tgatcaaaga
     1621 tgcacttgac agggggcttg cttctgtttc acttgccgat tgctatgttc cgtatacacc
     1681 gacggaagat atgcaggaag tatatgcgct ttggaagaaa atcgataagg tgcagcagtc
     1741 aacgatcgtt tttgaagacg gcgatctgga gcggacattg aatgtcggtg tgatcggtga
     1801 ctggatcgtt gctgatcaga atgatatgcc gatcctggac gaaaacggac agatccagct
     1861 ggatcctgaa aaagttgctg catttgccgg ggaactggcg gcattatttg atacctatgg
     1921 aaaagaccgc ctctggcata aaaaaaacgg gggaacggtt tggcttcaat attccggaag
     1981 cggttatgaa gtcgatcagg aagcagagat cgaaaaattg acggaagatg tactggccgg
     2041 aaaaacagaa acaagaaaac cgatctatga aagagaaggc agcggcaggg gaaaggacga
     2101 gatcggcaat acctatattg aaatcgacat gagcgcgcaa aagctctatt attttgtgga
     2161 tggaaggata aaacttacga ccgatgtggt gaccggcaat acgtcaaggg gacacggcac
     2221 gccggcaaag atctgcgcta tttatgcaaa acagaaaaac agggtgcttc gcgggcctaa
     2281 ctacgcagct tttgtttatt actggatgcc ggtatcgggc aatatcggta ttcatgatgc
     2341 cacatggcgt gatgaattcg gcggagaaat ttataagacc gacggctctc acggctgtat
     2401 caacctgccg aaagagaagg cggcaaagct ttatgaaatg gctgaaatag gaacgccggt
     2461 catattgtat tattaagttt ataaaagaat taacatatca tcaaaggagg agaaagagtt
     2521 gaaaagagtg tacaactttt cagcaggtcc ggcagtttta cctgaagaag tattaaaaga
     2581 agctgcagac gaaattttgg attacaaagg atgcgggatg tccgttatgg aaatgagcca
     2641 tcgatctaag gtctttgaac agatcatcaa tgaggcggaa gctgatctga gagagcttat
     2701 gagtattccg gacaattata aagtcctgtt tttacagggc ggggcaagtc agcagtttgc
     2761 catgattccg atgaatctga tgaagaacgg atccgcagac tatattgtaa cagggcagtg
     2821 ggcaaaaaaa gcctgcaaag aggcttctgc ttacggaaag gcaaccattg tagcttccag
     2881 tgaggataaa aatttttcct atataccgga ctgttccgat ttaccgatct ctgaagatgc
     2941 cgattatgtt tatatctgcg aaaataatac gatctacggc accaaattta aagaattacc
     3001 aaatacaaaa ggcaaaccgc ttgtggcaga tgtttcttcc tgttttctgt cagagcctgt
     3061 tgatgtaaaa aaatatggga tcatctacgg cggagtacaa aagaacatcg gaccggcagg
     3121 tgtcgtgatt gtgatcatac gggaagacct gattacggaa gacacgctta aaggcacacc
     3181 gaccatgctg aaattcaaaa cgcatgcgga tgcggcgtcg ctttataata cgcctcctgc
     3241 atacggtatt tacatttgcg gtaaagtgtt caaatggctt cttaaaatgg gcggtctgac
     3301 tgccatgaag gaacgaaacg agaaaaaggc aaaactttta tatgatttcc ttgatcagag
     3361 caaattgttc ataggaaccg tggaaaaaga ttcccgttcg cttatgaatg tgccgtttgt
     3421 tacaggcgat caggagttgg atcaaaaatt tgtaaaagaa gcgaaagaag ccggatttga
     3481 aaatttaaaa ggacatcgga gtgtcggcgg aatgcgcgcg agcatctata acgcaatgcc
     3541 gttggaaggc gttgcaaagc ttgtggagtt catgaggaaa tttgaggagg aaactattaa
     3601 aaatgtttaa atatgcttgt ttaaatccta ttgcaccgat cggtcttgac cagtttacgg
     3661 ttgattacga aaaatgcgat gatataacat ctgccgattg tctgcttgtt cgaagcgctt
     3721 ccatgcatga taaggaactg ccgcagacgc tgcttgcggt tgcaagggca ggggctggag
     3781 tcaataatat tccgcttgac cgatgtgcgg agaatggtat cgttgtattt aatacgccgg
     3841 gcgccaatgc gaatggtgtc aaagagcttg tgattgccgg tatgctgctt gcgtccagag
     3901 atattgtggg cggcatcaac tgggtaaaaa a
//
LOCUS       DAOSYE010000081         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16093_length_3919_cov_3.289337, whole genome shotgun sequence.
ACCESSION   DAOSYE010000081 DAOSYE010000000
VERSION     DAOSYE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16093_length_3919_cov_3.289337"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLU43_02225"
     CDS             <1..1018
                     /locus_tag="PLU43_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO27255.1"
                     /translation="MGGGHDEREQTLNQLLVEMDGFGINEGIIVMAATNRVDILDPAI
                     LRPGRFDRKVGVGRPDVKGRLEILKVHSRNKPLGDDVDIEQIARTTAGFTGADLENLM
                     NESAIYAARENRAYLQQADIKKAFVKVGVGSEKKSKIITEEDKKITAYHEAGHAILYH
                     VLEGVGPVYMVSIIPTGPGALGYTMPLPERDDVHRTKKQMLNDIMVALGGRIAEQIVF
                     DDVTTGASQDIKQATKTARDMVTKYGMSDNLGMINYAGDDDEVFIGRDLAHTRSHSEA
                     IASEIDEEVKAIVGSCYKKASGLIKENRKILDSCAQLLLEKEKINRAEFEALFEEENA
                     TETV"
     gene            1379..3481
                     /locus_tag="PLU43_02230"
     CDS             1379..3481
                     /locus_tag="PLU43_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOO27256.1"
                     /translation="MDSLWFASEYEKGLYLSSMRPVFNKKALFCDYSEDYIIPLEPSP
                     YGLVKLRFRTGKNNVDYVFLLCNGERLLMEKLKTVDDFDYYEFEYQLNNERITYCFEI
                     NCGKLKCYYSMKGVSKHIDTKYKFSLCPGFKTPAWAKGAVIYQIYIDRFYNGDKSNDV
                     ETNEYRYIGEGTVRVEDWNKYPAVMGVREFYGGDLQGVIDKLDYLSELGIDAIYFNPL
                     FVSPSNHKYDIQDYDYIDPHVGKIVDDRGELLQPGSERFENRFATKYINRVTNKKNLE
                     ASNELFIKVVEEAHKRNIKVILDGVFNHCGSFNKWMDRERIYENSEGYEKGAFVDENS
                     PYRNFFKFHAEGWPYNAAYDGWWGHDTLSKLNYEGSSCLYEYMLHIARKWVSPPFHAD
                     GWRLDVAADLGYGPEINHRFWKDFRRAVKEANPDAIIIAEHYGDPSAWLQGDEWDTVM
                     NYDAFMEPVTWFLTGMQKHSDDYREDLRGNAGFFKNMIEVNTARFSAPSLFVAMNELS
                     NHDHSRFLTRTNRVVGRVSNKGSQAADCGVNKAVFREAVLIQMTWFGAPTIYYGDEAG
                     VCGFTDPDNRRTYPWGNEDKELIRFHKELIRIHKENQVFKTGSGKWLALDYNILAYGR
                     FSIREQCLILVNNNDCEKKIAMKVWAIGVPREGIVRSLIYTDEKGFHMRPEEYIITKG
                     RLEMILPKTSAIILKVVK"
     gene            3513..3752
                     /locus_tag="PLU43_02235"
     CDS             3513..3752
                     /locus_tag="PLU43_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27257.1"
                     /translation="MRLMEFKMERHGLLKEGQQITVTEGVLPASYYYTIDPSLAMSAN
                     YQFRERLKTREGIVKQIEEKPRGYYVTAAFEEEEP"
     gene            3848..3919
                     /locus_tag="PLU43_02240"
     tRNA            3848..3919
                     /locus_tag="PLU43_02240"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3881..3883,aa:Phe,seq:gaa)
BASE COUNT         1160 a          703 c         1078 g          978 t
ORIGIN      
        1 catgggcggc ggacatgatg aacgtgaaca gacgctgaac cagcttcttg ttgaaatgga
       61 tggatttggt atcaacgaag gtattattgt catggcggcg acgaacaggg tggatatact
      121 ggatccggca attctgcgtc cgggccggtt tgacagaaag gtcggtgttg gcagacctga
      181 tgtgaaaggg cgtctggaga tcctgaaagt gcattccagg aacaagccgc tcggagacga
      241 tgtggatatt gaacagatcg caaggacgac cgcaggcttt acgggagcgg atcttgaaaa
      301 tctgatgaat gaatccgcga tctatgctgc ccgtgagaac agggcttatc tgcagcaggc
      361 ggatattaaa aaagcgtttg tcaaggttgg agtcggctct gagaaaaaga gcaagatcat
      421 tacggaagag gataagaaaa tcactgctta ccatgaagca ggacatgcga tcctgtatca
      481 tgtgctggag ggtgtcggac cggtctatat ggtatccatc attcctacgg ggccgggggc
      541 gctcggctat acgatgccgc tgccggagcg tgatgatgtg caccgtacga aaaagcagat
      601 gctgaatgat atcatggttg ctttgggagg ccggatcgct gaacagatcg tgtttgacga
      661 tgtgacaacg ggtgcctcgc aggatatcaa gcaggcaacg aaaacggcca gggatatggt
      721 cactaaatac ggaatgagcg ataatcttgg gatgatcaat tatgccggag acgatgatga
      781 agtgtttatc gggagggatc tggcacatac gagaagccac agcgaggcga ttgccagcga
      841 gatcgatgaa gaagtaaaag cgatcgtagg aagctgttat aaaaaggcat ccggtctcat
      901 caaagagaac aggaagatcc tggatagttg tgcgcagctt ttgcttgaga aggaaaaaat
      961 caacagagcg gaatttgagg cattgtttga agaagagaac gccacggaga cagtataggc
     1021 ccaaaatttg tgcatatgta ccaaaaatga atatcgaaat ttgtaaagtt tgtgcatcct
     1081 aagactagat tttgcagggg gttgatgcta caatacatct tgtaaccccc caatacattg
     1141 tatagttttt aactataccc cataagaaag aaataccttc tccaaaaaag acagcattcg
     1201 tgaggctgtc ttttttactg ctcatacaaa atgtcggttt ggcagcctgc caataccata
     1261 tttggtagga gatgtgagaa aggcttgtac atagtgtatg aaaaatgtat aatatgaatt
     1321 atgatacaga tttagtagaa cgttttagta acgcagaaat taataaagga gttgtagtat
     1381 ggattccctg tggtttgctt cagaatatga aaaggggctg tatttatcaa gcatgcgtcc
     1441 cgtatttaat aaaaaggctt tgttttgtga ttactcggag gattatatca ttccgttaga
     1501 gccaagtcct tatggacttg taaagcttcg tttcagaaca ggtaaaaata atgtggatta
     1561 tgtttttctg ctgtgcaacg gagagcggct tctgatggag aagctgaaaa cagtagatga
     1621 ttttgattat tatgagtttg agtaccaatt gaataatgag aggatcacct attgctttga
     1681 gatcaactgc ggaaagctga aatgctatta cagtatgaaa ggtgtttcaa aacatattga
     1741 tacgaaatac aagttcagtc tgtgtccggg atttaaaaca cctgcatggg caaagggcgc
     1801 tgtgatctat cagatctata ttgacagatt ttataacggc gataagagca atgatgtgga
     1861 gacgaacgaa taccgttata tcggagaggg aaccgtccgg gtagaggact ggaacaagta
     1921 tccggcagta atgggtgtcc gtgagtttta cggcggcgat ctgcagggag tgatcgataa
     1981 gctcgactat ttatcagagc ttgggatcga tgcgatttat tttaatccgt tatttgtatc
     2041 gccttctaac cataaatatg atattcagga ttatgattat attgacccgc atgtcggaaa
     2101 aattgtagac gacaggggag agcttcttca accggggtca gagcggtttg aaaaccgttt
     2161 tgccacaaag tatatcaatc gcgttacaaa caaaaagaat ctggaagcga gcaacgagct
     2221 gtttataaaa gttgtggaag aggcacacaa gcggaatatc aaggtcattc tggacggtgt
     2281 gttcaatcat tgcggctcct ttaacaagtg gatggacagg gaaagaattt atgaaaattc
     2341 agaaggctat gaaaaaggtg catttgtaga tgaaaacagt ccgtacagaa attttttcaa
     2401 atttcatgcg gagggctggc cttataatgc agcttatgac ggatggtggg gacacgacac
     2461 actgtcaaag ctgaattacg aaggttcttc ctgtttatat gaatatatgc tgcatattgc
     2521 gcgcaaatgg gtgtctccac cctttcatgc ggatggctgg agactggatg tggcggccga
     2581 ccttggctat ggtccggaaa tcaaccaccg gttttggaaa gatttcaggc gtgccgtaaa
     2641 ggaggccaac ccggatgcga tcattattgc cgagcattat ggggatccgt ctgcctggct
     2701 gcagggagat gaatgggaca cggtcatgaa ctacgatgca tttatggaac cggtcacatg
     2761 gtttctgacc ggcatgcaga agcacagcga tgattatcgg gaagacctgc gcggaaatgc
     2821 gggatttttt aagaatatga tcgaggtcaa taccgcccga ttttcagcgc cgtctctgtt
     2881 tgttgcaatg aatgaattgt ccaatcatga tcattccaga tttttgacaa ggacgaaccg
     2941 ggtcgtgggc agggtcagca ataaaggctc acaagcggct gactgcggag tgaataaagc
     3001 ggtgtttcgg gaagcggtcc tgatccagat gacatggttt ggcgcaccga cgatctatta
     3061 cggcgatgaa gccggggtct gcgggtttac cgatcctgac aacaggcgga catatccgtg
     3121 gggaaatgag gataaggagc tgatccgttt tcataaggaa ctgatccgca ttcataaaga
     3181 gaaccaggtg tttaagaccg gttccgggaa gtggcttgcg ctggattaca atattcttgc
     3241 atatggacgt ttttcgatac gggagcagtg tctgatcctt gtaaataata atgactgcga
     3301 gaaaaagatc gcaatgaagg tatgggcgat cggcgttcca agggaaggga ttgtacgcag
     3361 cctgatctat acggatgaaa aaggctttca tatgaggccc gaagagtata tcataacaaa
     3421 aggcagactg gaaatgatcc tgcctaaaac ttccgcaatt atactgaagg tcgtcaaata
     3481 gacttacggc cggaaatgac aggtgattta ttttgagact aatggagttt aaaatggagc
     3541 gtcacggact gctgaaagaa ggacagcaga tcacagtgac ggaaggggtt ctgccggcaa
     3601 gctattatta tacgatcgat ccgtcgctgg cgatgtcggc aaattatcag ttccgtgaac
     3661 ggctgaagac gagagagggt attgtgaaac agatcgaaga gaagccgcgg ggatattatg
     3721 tgacggcagc ctttgaagaa gaggagccgt aaaagaaaat aaaaaaaaat agaaaaaata
     3781 cttgccaaag tgttgggaaa tgtatataat agcatttgcg agtgtcgcac cgaacgaaga
     3841 ggtatttgcc cagatagctc agttggtaga gcagaggact gaaaatcctc gtgtcactgg
     3901 ttcgattccg gttctgggc
//
LOCUS       DAOSYE010000082         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16167_length_3908_cov_3.500908, whole genome shotgun sequence.
ACCESSION   DAOSYE010000082 DAOSYE010000000
VERSION     DAOSYE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16167_length_3908_cov_3.500908"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PLU43_02245"
     CDS             complement(<1..1545)
                     /locus_tag="PLU43_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO27258.1"
                     /translation="MSSTKKTITGAEASSFIVRILVSAYTILLFILLPLCYRNNYFDI
                     GDFKYELFFNMTGCLFILLVPALLFYLFTHIHSKTGNTRTVKQFFSDLSVIDRFVLLY
                     LAACLITFFLSPYNTWTFFTKDASNPAWDGYDGWCMGLKSQLMFIALYFLVSRFFIKS
                     WKADLLASILGASSIVFLLGILHRFLIDPLALYEGIDSSYHILFLSTIGQATWYSSYL
                     CTVFPIGLFLFWYSDKPLVRILTGIYTAIGFASLVTQNSDSAFLAMAAFLVLLFALSF
                     QSAKYLQRFLEILLICGLSMRVIGVLQLAFPDRAVPLETLSVFLSQNPLLWIILFIIA
                     LLYFALASANARDIFKVEKVRFIRTLGFILLAVVIPLVVLCIVLTTTGHLPSWLSALK
                     NYDYMIFNENWGNGRGFTWKYSASMFAEYPLKLKLFGIGSDCFASYTYDYHAADVSVK
                     WGDSILANAHNEWFNMLLTTGIFGLFAYAGIFISAFVIFIKEKEKDLFLTAAAACIIS
                     YIFHNLF"
     gene            complement(1624..3231)
                     /locus_tag="PLU43_02250"
     CDS             complement(1624..3231)
                     /locus_tag="PLU43_02250"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOO27259.1"
                     /translation="MNDQEHINLTDSDIDFDFLKKEPPKEATRQPELKKASYKILISD
                     DDDEIHKMTKLVLKGFRMEGVGIEFLDAYSGKETIEILNKVPDIAVVLLDVVMEEPDS
                     GLKVVRYIRETLGNNLTRLILRTGQPGVAPEEKVIVDYEIDDYKAKSELTVQKLMSTM
                     YVCLRAYKNIKALDRHRKGLRRVIHASHDLFKYHSFTEFLNGMLQQVIALYDVEVDSF
                     YVRDERYTMNGMAFVKILDSAKVLAGTGKFEKIIGQSIDIDTLPKDLRKLIMRFENTE
                     DSEMVIREGRYLGIYKQNFDKLVKNYIVLEADVSNENIELIKLFLTNFSLAIDNFVLN
                     VNANETQKEIIFRVSEVVENRANDSTGQHLKRVSKITELLAKNLGFEKDMIEAVTLAS
                     VMHGVGKIGIKDSILLKPGKLTKEEFDEIKTHTTIGYNILKDSKLPVLKLAAKIALHH
                     HERFDGKGYPAGCSGTEIPKECAIVAVADVFDALLSKRCYKDKWKFEDVISYFEKERG
                     HQFTPEVVDALMDNLDQVYEIIEAYPD"
     gene            complement(3233..>3908)
                     /locus_tag="PLU43_02255"
     CDS             complement(3233..>3908)
                     /locus_tag="PLU43_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO27260.1"
                     /translation="LGIGVTTSTYMQKINRDCRKSMAEGRCTRQTLLDYMETMDESLD
                     LLQFNLERGSNIIQSFKKIAVDQTSESLKEFDLNEYIQGIVTSLTHEYKNTGHQITVD
                     CESELTIKSYPGVFAQILTNLIMNSLNHAFKDKKNGRMLITAKKEETNLILVYSDNGC
                     GLNEEQKANLFTPFYTTSKQMGGSGLGLSIIYNLVTQKLNGSIKADSQENAGITFTIV
                     VPLEVN"
BASE COUNT         1168 a          798 c          782 g         1160 t
ORIGIN      
        1 gaacaggtta tgaaaaatat atgaaatgat acaggcagcg gcagcggtca ggaacagatc
       61 tttttctttt tcctttataa aaataacaaa cgcggatatg aaaatccctg cgtaggcaaa
      121 cagtccgaaa ataccggtag tcagcagcat attaaaccac tcattatgcg catttgccaa
      181 tatggagtct ccccatttga cagaaacgtc ggccgcatga taatcatacg tataagaagc
      241 aaaacagtcg gaaccaatgc cgaacagttt gagttttaat ggatattccg caaacatgga
      301 agcactgtat ttccatgtga agcctcttcc atttccccag ttttcattaa atatcatata
      361 atcataattt tttagcgcag aaagccagga aggcagatgt cctgtagtag taaggacaat
      421 gcataaaaca acaagaggaa taaccacagc aagcagtata aatccaagtg tccgtataaa
      481 tctgaccttt tcgactttga aaatgtctct ggcatttgca gatgccagcg caaaatagag
      541 aagagcgatt ataaataata tgatccacag aagaggattt tgggatagaa agacagagag
      601 tgtctcaaga gggacggccc tgtcagggaa agccagctgt aaaacaccga tgacacgcat
      661 tgacagaccg catatcagca gaatttccaa aaaacgctgc agatattttg cggattggaa
      721 ggagagggca aacagcaata ccaaaaaagc ggccattgcc agaaaggcgc tgtcactgtt
      781 ttgtgtgaca agcgaagcga agccgattgc ggtataaata ccggtcaata tacgcacaag
      841 aggcttgtcg ctgtaccaga acaaaaacag tccgatcgga aataccgtac acaaataact
      901 ggaataccag gtagcctgtc cgatcgttga cagaaaaaga atatggtaag aggaatcgat
      961 tccttcatat aatgcaagcg ggtcaattaa aaaacggtgc agaatgccaa gcaggaatac
     1021 aatggacgag gcacctagaa tgcttgccag cagatctgcc ttccagcttt ttataaaaaa
     1081 acgggaaaca agaaaataaa gcgcaataaa catcaactgg ctttttaatc ccatacacca
     1141 gccgtcataa ccgtcccatg ccggattgga cgcatctttc gtaaaaaatg tccatgtatt
     1201 atacggactc aggaaaaaag tgatcagaca tgcagccaga taaagcagta cgaatcggtc
     1261 gataacggaa agatctgaaa aaaactgttt tacagttcgt gtattccctg ttttggaatg
     1321 aatatgcgtg aacagataaa acaacagtgc cggaacaagc agtatgaaca ggcaccccgt
     1381 catattgaaa aaaagttcat atttgaaatc tccaatatca aaataattat ttctgtaaca
     1441 cagaggaaga agaataaata aaagaatcgt ataggcggaa accagtatac gaacgataaa
     1501 tgaagaagct tccgcaccgg ttattgtttt ttttgtggat gacataatat acgctccgtt
     1561 tctttttgat cactgtttcc attataggcg gatgcgtttt aaaatacaag tgcaggcatt
     1621 ttgtcagtca ggatatgctt caatgatttc ataaacttga tccagattat ccattaatgc
     1681 atcgacaact tcaggggtaa actgatgtcc ccgttctttt tcgaaatagg aaatgacatc
     1741 ctcaaatttc catttgtctt tataacagcg ttttgacaaa agggcatcaa atacgtcggc
     1801 aacagcaaca atggcacatt ctttcggaat ctctgtgcct gagcagccgg ccggataccc
     1861 ttttccgtca aatcgttcat gatggtgcaa tgctattttg gcagccagtt tcagtaccgg
     1921 taatttggaa tccttcagta tattatatcc gatcgttgtg tgtgtcttga tttcgtcaaa
     1981 ttcttctttt gttaattttc ccggttttaa cagaatcgaa tcttttattc cgatcttgcc
     2041 tacgccatgc atgacgctgg caagtgttac cgcttcaatc atatcttttt caaaaccaag
     2101 attttttgca agaagctccg tgattttcga tacacgcttt aaatgctggc ctgttgagtc
     2161 atttgcgcga ttttccacga cttcacttac acggaaaata atttctttct gcgtttcatt
     2221 tgcatttaca tttaatacaa aattgtcgat ggcaagagag aaatttgtga gaaacagctt
     2281 gatcaattca atgttttcat tgctgacatc agcttctaaa acaatatagt ttttgacaag
     2341 cttatcaaaa ttttgtttgt agatgccaag gtatcgccct tctcttatga ccatttcaga
     2401 atcttccgtg ttctcgaacc gcataatgag ttttcgcaga tcttttggaa gcgtgtctat
     2461 atcaatggac tgtccgataa ttttttcgaa ttttcctgta ccggcgagta cttttgcgga
     2521 atccagtatt tttacgaaag ccatgccgtt catcgtatag cgttcatccc tgacataaaa
     2581 ggaatcaact tcaacatcgt agagtgcgat cacctgctgg agcattccat ttaaaaattc
     2641 ggtaaaggaa tgatatttaa acagatcgtg tgatgcatga attacccggc gaagaccttt
     2701 tcgatgccgg tcgagagctt ttatattttt atatgcacgt aaacagacat acatggtact
     2761 catcagtttc tgtacggtca gttctgattt tgccttgtag tcatcgatct cataatcgac
     2821 aatcactttt tcttcgggag caacgccggg ctgtcctgtc cgaagaatca gacgggtcag
     2881 gttattccca agagtttcac gaatgtaacg gacaaccttt aaaccggaat caggttcctc
     2941 cataactacg tcaagcagaa caacggcaat atcaggaacc ttgttaagaa tttcaatggt
     3001 ttctttacct gagtaggcgt ccaggaattc aatccctacg ccttccatgc gaaagccctt
     3061 taatactaat tttgtcattt tgtgtatttc atcatcatcg tcggaaatca gaattttata
     3121 agaggccttt ttcagctcag gctggcgtgt tgcttccttt ggaggctctt tttttaaaaa
     3181 atcaaaatca atgtctgaat cagttaagtt gatatgttct tggtcattca ttctaattca
     3241 cctccagggg tacgacaatc gtaaaggtaa tgccggcatt ttcctgactg tctgctttga
     3301 tgcttccgtt tagtttctgt gtaaccagat tatagatgat actcaagccc aatccgctgc
     3361 cccccatttg tttgcttgtt gtataaaagg gtgtgaacag atttgctttt tgttcttcat
     3421 ttaatccgca gccattgtcg gagtaaacaa gaatcagatt ggtttcttct tttttcgccg
     3481 taataagcat gcgtccgttt tttttatctt taaaagcatg attaagagag ttcatgataa
     3541 ggtttgtcag tatttgtgcg aatacaccag gatagctttt aatggtcagt tcgctttcac
     3601 agtcgaccgt gatctgatgt cccgtatttt tatattcatg tgtcagactg gtgacgattc
     3661 cctgaatgta ttcatttagg tcgaattctt taagagattc ggaagtctga tcaactgcga
     3721 tttttttgaa gctttgaatg atattgcttc cgcgctctaa attgaattgc agcagatcca
     3781 gactttcgtc catggtttcc atataatcaa gcagagtctg ccttgtacac cggccttctg
     3841 ccatggattt tctgcaatcc cgattgatct tctgcatata agtggaggtg gtgacaccga
     3901 tccctaaa
//
LOCUS       DAOSYE010000083         3903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16197_length_3903_cov_2.298337, whole genome shotgun sequence.
ACCESSION   DAOSYE010000083 DAOSYE010000000
VERSION     DAOSYE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16197_length_3903_cov_2.298337"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PLU43_02260"
     CDS             complement(<1..1304)
                     /locus_tag="PLU43_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOO27261.1"
                     /translation="MPLNKKLIRLTYTQYIALGFLFLILTGSLLLTLPFMNRSGQWTP
                     FLDALFTATSATCVTGLVVHDTYTYWNTAGQLVILLLIQIGGVGFMTVIALFSLIARR
                     QITLHERKLLMQSAGAIKVGGIVQLVKQILLVTLCIEGAGTLLLATRFCRDPKLGFGR
                     GLYFSLFHSISAFCNAGFDLMGAKEKYSSLTSYMMDPVVNFTVIILIILGGLGFIIWN
                     DLGRNKWHFQRYALHTKLGLTATAILIVGGTILFFIFEFSRTQSGMNLGQRLMVSLFD
                     AITPRTAGFNTSDMTHFSDSSIMLTIVLMFIRANPGSTAGGIKTTTLLVLILSISATC
                     NHSSSISIFKRKLEDNIVRQAAAIATIYLFAIFFCSMIICAIEPYSMKEVLFETTSAA
                     GTVGLTLGITPFLGTVTRLLLILLMYGGRIGGLTLALVLAER"
     gene            complement(1394..2797)
                     /locus_tag="PLU43_02265"
     CDS             complement(1394..2797)
                     /locus_tag="PLU43_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO27262.1"
                     /translation="MLAFSLWESPYYEHWYGCDASFFSMVGRGITQGMVPYRDFFDLK
                     GPYFFFIQALGQLLHKNRLGAFLIEIPFLFFSLLLLYELAKLYLSKKKSIAVLCIFLF
                     MHISTLWGGNTLEEFCLPLSLLCLYLTARHLKKTGLPIWQLPKASAFIIGICFGIVAF
                     SKITVAAPVLGIVCAILFLDLAGKKPGSLLVFLLFFILGTAAAALPVFLYFGYHHAIG
                     EMLYCVFSFALKRSVDFSEKFNIVWEIKLSGCIFAFLFACLHRRQLSGATRIILVAMS
                     AATYLLLHLGNPFIYYFTTVYPSSLFAFILLLSVYDPLILFKNVRQGICLSVLGVCLF
                     YYATCSMDTVRTFLYGKSNSYHEDYYKDASDLATLIPEWERDQVYCFSTDMTWFEANQ
                     ILPCYKYQVNLQFFIALDPQIKGDIITYLNNTPPKWLVVGSSFQNEIPDLFEIVSEKY
                     DCICSNSSGDLYLLRND"
     gene            3042..3494
                     /locus_tag="PLU43_02270"
     CDS             3042..3494
                     /locus_tag="PLU43_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27263.1"
                     /translation="MYGQEQTTREMIIREYREDVEKLLKYLPWLLEKEAKDVAGYYEG
                     DGTQKLIKIPVYDSTLLAFVKEAEKTKFMDKNYPYVYTRHRIKSCGDERRLLQQAKIQ
                     DIDIFKGILSKYVLEGKRKSSCWRDAVGEKIFTTALSCLKELFFSHAA"
     gene            3550..>3903
                     /locus_tag="PLU43_02275"
     CDS             3550..>3903
                     /locus_tag="PLU43_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27264.1"
                     /translation="MEEKYLLVDESIAGFLEAVYLGRPDDMEPLSKAIGVLANIMRIS
                     QICAFSTIQVEQEVTVYKRYDTKLLNMPDWEYTESVYAPEDVRIRFWKEEDREWQDPD
                     FINMKNLARFIILYVG"
BASE COUNT         1280 a          759 c          954 g          910 t
ORIGIN      
        1 cttctttcgg cgagaacaag cgccagtgta aggccgccga tcctgccgcc gtacatcagc
       61 agtatcaaaa gaagtcttgt aacagtacca aggaacggtg tgatgccaag ggtaagacca
      121 actgttccgg cagccgaagt cgtctcaaac agaacctctt tcatggaata aggctcaatt
      181 gcacagatga tcatggagca gaaaaaaatg gcgaacagat agatcgttgc aatggctgcg
      241 gcctgtctga caatgttatc ttccagtttt cttttaaaaa tactgatgct ggaagaatga
      301 ttgcacgtag ctgaaatact taggatcaaa acaagaagcg ttgttgtttt gataccgccg
      361 gcagtagaac cgggattcgc tctaatgaac atcaaaacga tcgtaagcat gatggaactg
      421 tcgctaaagt gagtcatatc gcttgtatta aaaccggcgg ttcttggagt gatggcatca
      481 aaaagggaaa ccatgagccg ctgtccaaga ttcataccgc tttgtgtacg ggagaattca
      541 aaaataaaaa ataaaattgt accgccgaca atgagaatag ccgtagcggt caatcctaat
      601 tttgtatgca gtgcgtaacg ctgaaaatgc catttatttc ttccaaggtc attccagatg
      661 ataaaaccga gaccaccgag aatgatcagg atgattaccg tgaaatttac aacaggatcc
      721 atcatatagg aggtcaggga ggaatacttt tcttttgcgc ccatcagatc aaagccagca
      781 ttgcaaaaag ctgaaatgga atgaaacaga gaaaaataaa gacctcttcc gaagccaagt
      841 ttggggtcgc ggcagaatct ggtcgcaagc agcaatgtac cggcaccttc gatacacagc
      901 gtaacaagaa ggatctgttt gaccagctgt acgataccgc ctactttaat ggcacctgca
      961 gactgcatca gcagctttcg ctcatgcaaa gtaatctgac gtctggcaat cagagaaaac
     1021 agcgcgatga cagtcatgaa tccgacaccg ccgatctgga tcagcaggag aatgacaagc
     1081 tgtccggcag tattccagta cgtatatgta tcgtgaacga ctaatccggt gacacaggtc
     1141 gcggaagttg ccgtaaataa tgcgtcaaga aaaggcgtcc attgtccgga gcgattcata
     1201 aaaggaagcg tcagcagcag gctgccggtc aatatcagaa acagaaagcc aagagcaata
     1261 tattgtgtat aagttaaacg aattaacttc ttgtttagcg gcataatcac aaaccatcca
     1321 atcaaaaatg ctgataagca aagtataaca taaacaaaaa ttaatacaag gaaagaattg
     1381 agaatttaat catttaatca tttcttaaca gatataagtc gccggaactg ttcgaacaga
     1441 tacagtcgta cttttcgctg acgatctcaa aaagatcggg gatctcattt tgaaaggaag
     1501 aaccgacaac aagccatttc ggcggcgtgt tattcagata ggtaataata tcgcccttga
     1561 tctgcggatc cagtgcaatg aaaaactgca gattcacctg atatttgtag cagggcagga
     1621 tctgatttgc ttcaaaccat gtcatatccg tgctgaaaca atagacctga tcacgttccc
     1681 attccgggat cagggtggcg aggtccgatg cgtctttata atagtcttca tggtaggaat
     1741 tgcttttgcc gtacaggaaa gtacggaccg tatccatgct gcaggtggca taataaaaca
     1801 gacagacgcc aagcacggac agacagatcc cctgccggac atttttaaac aggatcagcg
     1861 gatcgtaaac agagagcagc agaataaagg caaacagtga agaaggatag acggttgtaa
     1921 agtagtagat aaacggattg ccaaggtgga gcagcagata ggtcgctgcc gacattgcaa
     1981 ccaggatgat gcgggtcgcg ccggataact gacgtctgtg cagacaggca aataaaaatg
     2041 caaaaatgca gccggacaat ttgatctccc acacgatatt aaatttttcg gagaaatcga
     2101 cagaccgttt cagagcgaag ctgaatacac agtagagcat ttccccgatc gcatggtgat
     2161 aaccgaaata aagaaaaaca ggaagcgcgg ctgctgcggt cccaagaata aaaaacagca
     2221 gaaaaacaag cagggaaccc ggttttttac cggccagatc taaaaacaga atggcgcaga
     2281 cgattcctaa aacaggagca gcgaccgtaa tcttagaaaa agcaacgata ccaaagcaga
     2341 tgccgatgat aaaggccgag gcttttggaa gctgccagat cggcagtccg gtttttttca
     2401 gatgccgtgc tgtcagataa aggcagagca ggcttaacgg aaggcagaat tcttcgagcg
     2461 tattgccgcc ccataatgtg gaaatatgca taaacaggaa aatacagagg accgcaatgg
     2521 atttcttttt ggaaagatag agtttggcaa gctcatagag gaggaggagc gagaaaaata
     2581 aaaacgggat ctcgatcaga aacgcgccga gacggttttt gtggagcagc tgtccgagcg
     2641 cctggataaa gaaaaaataa ggtcccttta aatcaaagaa gtcccggtag ggaaccatgc
     2701 cctgtgtaat accgcgtccg accatggaaa agaaagatgc gtcacagcca taccagtgtt
     2761 cataataggg actttcccaa agggagaatg caagcataaa gacagcggag acaaggaaga
     2821 gtaaaagata ctccagtcct ttttgaatat atgcggttct cttagggttt tcagggaaat
     2881 tcactgtctg ttaccatgct ttctgtgcgt cgttattggg attttttatg taaatcaaat
     2941 ctttacatcg tgacacgtat agattataat aaaaaaagga ttattttgca attcatttgc
     3001 tgacaaaatg ataaaaacgg acgtaaggag ggtatttcgt aatgtatggg caggagcaga
     3061 caacacgtga gatgatcatt agagaatatc gtgaagatgt agaaaaatta ttgaaatatc
     3121 tgccatggct tttggaaaaa gaagcaaaag atgtcgcagg ctattatgaa ggagacggca
     3181 cacagaaact gatcaaaata ccggtgtatg attccacgct gcttgcattt gtaaaagaag
     3241 cggaaaagac gaaatttatg gataaaaatt atccttatgt ttatacgcgc cacagaataa
     3301 aaagctgcgg ggatgaaaga agactgctgc agcaggccaa aatacaggat attgatattt
     3361 ttaaaggaat cttaagcaaa tatgtgctgg aggggaaacg gaagtcttcc tgttggaggg
     3421 atgctgtcgg agaaaagata tttactactg cattaagctg cctgaaggaa ctttttttca
     3481 gtcacgcagc gtgatttcgc aaaggcgcat ggtttggcgg atgttttgta atcagcgggg
     3541 taaagacaca tggaggaaaa atatttatta gtcgatgaat ccatagccgg ttttctggaa
     3601 gcggtatatc ttgggcggcc tgatgatatg gaacccttat caaaagcgat cggtgttctt
     3661 gcaaatatta tgcggatcag tcagatctgc gcgttcagta cgatccaggt cgaacaggaa
     3721 gttactgttt ataagcgcta tgatacgaaa ttactgaata tgccggattg ggaatatacg
     3781 gaaagcgtct atgcaccgga agatgtcagg atccgcttct ggaaagaaga ggacagggaa
     3841 tggcaggatc cggattttat caatatgaaa aacctggcgc ggtttattat tctgtacgta
     3901 ggc
//
LOCUS       DAOSYE010000084         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16255_length_3894_cov_3.548841, whole genome shotgun sequence.
ACCESSION   DAOSYE010000084 DAOSYE010000000
VERSION     DAOSYE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16255_length_3894_cov_3.548841"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLU43_02280"
     CDS             <1..688
                     /locus_tag="PLU43_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO27265.1"
                     /translation="KLPNVSFEETLISCLEKKAYGTQPGHAQFYYPKKYGYGELWSRM
                     AKALGDRILYDTKVREIDFDKKTVNGQFQAEVIVITIPWNVFETYSGLPASYRQAIDT
                     LKYSSVVIEYYPENLATKAHWIYYPDLQLSYHRILVRHNFCPGTDGYWTETNLTRFCP
                     DKKEEGMYFINEYAYPLNTIGKQEKMAALLNWAETKQVYGLGRWGEWQHYNSDVVTAL
                     AIKKADDFCK"
     gene            722..1447
                     /locus_tag="PLU43_02285"
     CDS             722..1447
                     /locus_tag="PLU43_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO27266.1"
                     /translation="MKKTVVVMPVANEESTMEKVLTEIMELPYDNLYVYPVIDSYSKD
                     RTEEIIRRMEEKYERIKLIFYKESKGVITCYLYGFKMALQDNAERIIEMDGGGSHHPK
                     EIPQFIEKLDEGYDCVWGSRFIWGGDITNQPLYRRILSSGGTWLSNLVLGTKLKDMTS
                     GFEAFQREVLERMDLDAFLSRGHMYQTEMRFYCRQLHTIEVPINYTGSKSSIKLKSVT
                     EALKLLFQLKKNESKVMPKEKQA"
     gene            1444..3102
                     /locus_tag="PLU43_02290"
     CDS             1444..3102
                     /locus_tag="PLU43_02290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO27267.1"
                     /translation="MSFQNVLQNKKARIREYLSDDTNKYKFGSLLVFIFIITLLAWQS
                     DDAYHAYVMAKNLVLGNGFVYNIGERVSATSCPLFTLIIAGGYFIFRKMFLVSLLICI
                     IFSSMAYHIVMKYFCRTKKQVIYTFLMLIGSTCFISYTTSGLENCLLFFLAALFLRLY
                     FSQEAYNSRRLFALALLVSLIAMTRMDAVLLFVPMAVYVFLAKRERVSFIKAVGIGVA
                     GLLPFIGWELFSVIYYGFPVPNTAYVKLGTDIPFKEYFIRGIQYLLTSAICDILLIAI
                     PVIVVLITLFLKKAKFIVCSAGIVCYVCYIIYIGGDFMLGRHFTVLFFMSVICYLQMQ
                     NTAFAGYHGKLKFEKIIVTVLVAGLIYTGTTRVITTQFLFGNNFNSPISDERGNYFQY
                     TSLFNNAISYLRTGKLVIRNAWNEQGIEEYREAGYHGGILKMVPGISIYYNSDMYLND
                     LYALGDPFLSKLPAVREENWRIGHMWRESPYGYNDTVLYGENVIENKDLHQYYEIIKL
                     ITRGDIWDKTRLKAVLDMNLGKYEYLVENYKSTLDDNCMQKPKE"
     gene            3114..3746
                     /locus_tag="PLU43_02295"
     CDS             3114..3746
                     /locus_tag="PLU43_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOO27268.1"
                     /translation="MVKKMKINTFLVSLLYVVLGVIMILFPKTTMKTICYIFAGILIL
                     IGLVYTFSYFKRNIVESYYRYDLVYGLVTILCGVLVISKVDLLIDLIPIVMGLLIFGN
                     GIIKLQHAIDLKRVDFSGWFYVLIFSLLCISIGAVLIVQPAFIAETVTMIIGISFTFS
                     GITDLITLLLLSKKIKDFKNGKAVIIDDGPVDESETVDERNDETDRQDEA"
BASE COUNT         1164 a          612 c          921 g         1197 t
ORIGIN      
        1 taagcttcca aatgtatctt ttgaagagac gctgatcagc tgtctggaga aaaaggcgta
       61 cggtacacag ccgggacacg cgcagttcta ttatccgaaa aaatacggat acggagaatt
      121 gtggagccgg atggcgaaag cacttggcga cagaatttta tatgatacaa aggtcagaga
      181 gatcgatttt gataaaaaga cggtaaacgg acagtttcag gctgaagtga tcgtaataac
      241 gattccgtgg aatgtttttg aaacatacag cggccttcct gcgtcgtata ggcaggctat
      301 tgatacatta aagtatagtt ccgttgtcat cgaatattat cctgaaaatc ttgcgacaaa
      361 agcgcattgg atctattatc ccgatttgca gctttcctat catcggatac ttgtccgtca
      421 caatttctgt ccggggacgg acggatactg gacggaaaca aatctgacca gattttgtcc
      481 ggataaaaaa gaagaaggaa tgtattttat aaatgaatat gcctatccgc tgaatacgat
      541 cggcaagcag gaaaaaatgg ctgcgctgtt aaactgggcg gaaacaaagc aggtatatgg
      601 tcttggcaga tggggtgaat ggcagcatta taattcggat gtagtcacgg ccctggctat
      661 aaaaaaggca gatgattttt gtaaataaga aaccgggatg accgggattt gaggtaaata
      721 aatgaaaaaa acagtagtag ttatgccggt agcgaacgaa gagtctacga tggagaaagt
      781 tcttacggag atcatggagc ttccgtatga taatttgtat gtttatccgg tgatagattc
      841 ctattctaaa gacaggaccg aagaaattat tcgcagaatg gaagagaaat acgagcggat
      901 caagctgatt ttttataaag aatccaaagg agtcattacc tgttatttat atggatttaa
      961 gatggcgctg caggataacg cggagcggat cattgaaatg gacggaggcg gaagccatca
     1021 tccaaaggaa attccgcagt ttattgaaaa gctggatgaa ggctatgact gcgtatgggg
     1081 ctctcgtttt atatggggcg gagatattac gaatcagccg ctgtacagaa ggattttaag
     1141 ctctggggga acatggcttt ccaatcttgt cctcgggaca aaattaaaag atatgacaag
     1201 cggatttgag gcttttcaga gagaggtatt ggaacgcatg gatctggatg cttttttatc
     1261 ccgcggacat atgtatcaga ctgaaatgcg tttttactgc agacagctgc atacgatcga
     1321 ggttccgatc aattataccg gctccaagtc cagcattaaa ctgaaatccg tgactgaggc
     1381 attaaaactt ttatttcagc taaaaaagaa tgaaagcaag gttatgccaa aggagaagca
     1441 agcatgagtt ttcagaatgt tctgcaaaat aagaaagcgc ggatcaggga atatttgtcc
     1501 gacgatacca ataaatataa atttggttct ttactggttt ttatttttat tattacatta
     1561 ctggcatggc agagtgatga tgcgtatcac gcttatgtaa tggcgaaaaa tctcgtgctt
     1621 ggaaatggat tcgtttataa catcggagaa cgggtatccg cgacctcctg tccgttattt
     1681 acattaatta ttgcgggcgg ctattttatt ttcagaaaaa tgtttcttgt atctttgttg
     1741 atttgtatta ttttttcttc catggcgtac catatcgtga tgaaatattt ttgcaggaca
     1801 aaaaagcagg tcatttatac atttcttatg ctgatcggaa gtacctgttt tattagttat
     1861 acaacgagcg gactggaaaa ttgtctgctg ttttttcttg cagcattgtt tttaagatta
     1921 tatttttcac aggaagcata taacagcaga cggctttttg cattggcgct gcttgtttct
     1981 ttgatcgcaa tgacaagaat ggatgcggta ttgctgtttg ttccgatggc cgtatatgta
     2041 tttcttgcaa agagagaacg ggtatccttt ataaaagctg tcggaatcgg ggtggccggt
     2101 cttcttcctt ttatcggatg ggagctgttt tccgttattt attatggatt tcccgtaccg
     2161 aatacggcat atgtaaagct tggaacggat atcccgttta aggaatattt tatccgcggg
     2221 atacaatatc tgcttacgtc ggcgatttgt gatattcttt tgatcgcgat tccggtcata
     2281 gttgtattga tcacgctgtt tctgaaaaag gcaaaattta ttgtatgttc ggccggcata
     2341 gtatgttatg tttgctatat tatatatatt ggcggggact ttatgctggg ccggcatttc
     2401 acggtgttgt tttttatgtc ggtcatctgc tatctgcaga tgcagaatac ggcgtttgca
     2461 ggatatcacg gaaaactgaa atttgaaaaa atcatagtaa cggttcttgt tgcgggattg
     2521 atctatacgg gaacaaccag agttatcaca acacaatttt tgtttggaaa taattttaat
     2581 tcgccgatct cagatgagcg cggaaattat tttcagtata ccagtttgtt taataatgcg
     2641 atttcttatc ttaggacagg aaagctggtt atccggaatg cttggaacga acaggggatc
     2701 gaagaataca gggaagccgg ttatcatgga ggtattttga aaatggtgcc cgggatttcc
     2761 atttattata attcggatat gtatttaaac gatttgtatg cacttggcga tccgtttctt
     2821 tcaaagctac cggctgtcag ggaagaaaac tggcgaattg gtcatatgtg gagagaatcg
     2881 ccgtatgggt ataacgatac ggtgctttac ggggagaatg tgatcgaaaa caaagatctg
     2941 catcagtatt atgaaattat caagctgatc acacgtgggg atatttggga taaaacccgg
     3001 ttaaaagcag ttttagatat gaatcttgga aaatatgagt atctggtgga aaattataaa
     3061 agtacattgg atgataactg tatgcagaaa ccaaaagaat aaggaggctc caaatggtaa
     3121 aaaaaatgaa aatcaatacg tttttagtgt cattgctcta tgttgttctt ggtgtaatta
     3181 tgatattgtt tccaaaaacg acaatgaaaa caatctgtta tatttttgca ggaattttga
     3241 tattgattgg acttgtttat acgttttcct atttcaaaag aaatattgtg gaatcctatt
     3301 accggtatga tctggtatat ggacttgtaa caatattgtg cggtgttttg gttatttcta
     3361 aggtggatct gctgatcgat ctgattccga tcgttatggg tctcctgatt tttggaaacg
     3421 gaattatcaa gctgcagcat gcgatcgatt taaaaagagt tgatttcagc ggatggtttt
     3481 atgtattgat tttttcttta ttgtgtattt caattggagc ggttctgatc gtacagccgg
     3541 catttattgc ggaaaccgtt acgatgatta tcggaatcag ctttacattc agtggaatta
     3601 ccgatttgat tacattgctt ctgctttcaa aaaagataaa ggattttaaa aatggaaaag
     3661 cagtgatcat tgacgatggg ccggttgatg aatctgaaac agtagatgaa agaaacgacg
     3721 aaacggaccg gcaggacgag gcgtaggcaa tgagaaagaa aaagacgcat tatacactga
     3781 tttatgaata cggcagcgat attttaaaat cggaagtttt tcaggggcag gatcaattta
     3841 tcaatgtcat ttagataagc attttttaga taaaaatcgt atatcacatt caac
//
LOCUS       DAOSYE010000085         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16344_length_3882_cov_3.677554, whole genome shotgun sequence.
ACCESSION   DAOSYE010000085 DAOSYE010000000
VERSION     DAOSYE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16344_length_3882_cov_3.677554"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>539
                     /locus_tag="PLU43_02300"
     CDS             <1..>539
                     /locus_tag="PLU43_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521976.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HOO27269.1"
                     /translation="CIYGIGSPSDYMNMIVSLRPGMIKDRDAVIHELVNISYERSQID
                     FGRGKFRVHGDTIEIFPAWESDRAVRIEFFGDEIDRITQIDVLTGEKKAEMNHFAVFP
                     ASHYVIPREKIDRAAGTIEAELAERVRYFKREGKLLEAQRISERTGFDIEMLKETGFC
                     SGIENYSRHLYGGKAGDTP"
     assembly_gap    540..549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <550..1547
                     /locus_tag="PLU43_02305"
     CDS             <550..1547
                     /locus_tag="PLU43_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO27270.1"
                     /translation="PDDFLIIVDESHMTVPQIRGMYAGDQARKNSLVEYGFRLPSAKD
                     NRPLNFEEFEGKIDQMMFVSATPAEYEKTHELLRAEQIIRPTGLLDPEISVRPTQGQI
                     DDLIGEINKETAGKNKIMVTTLTKRMAEDLTEYMREVGIRVKYLHSDVDTLERAEIIR
                     DMRLDVFDVLIGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTVGRAARNADG
                     HVIMYADTMTDSMRHAIDETNRRRKLQQAYNEEHGITPQTIKKAVRDLISISREIAKE
                     EIRFEKDPESMNRKELEKLIEQISKKMKKAAAELDFETAAELRDKMISLKKQLKE"
     gene            1592..2356
                     /locus_tag="PLU43_02310"
     CDS             1592..2356
                     /locus_tag="PLU43_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOO27271.1"
                     /translation="MNKISIVVPTYNESENVGNLANQIDYALKSIPYEIIFVDDSTDH
                     TPEVIKRVQAENANVRMEHRESEKGLATAVLKGFSLASGDYIAVMDADLQHPPSILRA
                     MYAVMESGADFCVPSRFIPGGSDGGLGPYRKLVSGVARYIGKILLPCLRKITDPTSGL
                     FMIKKKVIRDCDLQPIGWKILVEVLAMGTYGNVVEIPYKFQARPAGESKLSSKVTAEY
                     LKQVCGLMKRGKKQKQVMVSRWTQEMIKTAEKKYDI"
     gene            2346..3824
                     /locus_tag="PLU43_02315"
     CDS             2346..3824
                     /locus_tag="PLU43_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOO27272.1"
                     /translation="MIFSSVLFLCIFLPVMIIGYYAVPKKFKNAYMLLGSLFFYTWGE
                     PKYIFLIIASVIGNYFFGLAIYRLAKKQEEKKFVYAKKAVLIVSLLCNLGVLVYFKYF
                     VLLVSTADELLHATLTIPEIVLPIGISFYTFKSISYVVDVYRTEGKKDEDGKTLTLVE
                     KNPIDLALYISMFPEILSGPISRYKDVKDSLKEPKLSGAAFSSGIERFIVGLAKKAII
                     ANSIGEVADKIFAMDLSYAGTLAAWLGAILYTLQIYYDFSGYSDMAIGLGKIFGYDLM
                     ENFNYPYISKSITEFWRRWHISLSTWFRDYLYIPLGGNRRGNVYFNLFVVFLATGIWH
                     GAAWGFLIWGLWHGLFMLIERVFRNKKIKLQIPGFIKWVYAMLVVVFGWVLFKLEDIN
                     TALSYIGVMFHTKKYSYVAFSLRYYLDNKMIFILIAAVLAAVPWAQVLPRNFGSVIAA
                     ASIAKPAKPACIVKRILLIALLLLSMIFIVNSTYNPFIYFKF"
BASE COUNT         1077 a          739 c         1014 g         1042 t
ORIGIN      
        1 ttgtatttac ggtatcggca gcccttctga ttatatgaat atgatcgtat cactgcgccc
       61 tggtatgata aaggacaggg acgcggtcat tcatgagctt gtcaatatca gctatgagcg
      121 cagtcagata gattttggac gcggtaagtt tcgcgtgcat ggtgatacga tagagatatt
      181 tcccgcatgg gaaagtgacc gtgcagtcag gatcgagttt ttcggagacg aaatagaccg
      241 gatcacccag atcgatgtgc tgaccggcga aaaaaaggcg gaaatgaacc attttgcagt
      301 ctttccggct tctcattatg taattccgcg tgaaaagatc gatcgtgcgg cagggacgat
      361 cgaggcggag cttgcagagc gtgtcagata ttttaaaagg gaaggaaagc tgcttgaggc
      421 acagcgtatt tcggagcgaa ccggatttga tatcgaaatg ctgaaggaaa ctggtttctg
      481 ttccggcatt gaaaattatt cgcgtcattt gtatggcggg aaagccggag atacgccatn
      541 nnnnnnnnnt tccggatgac tttctgatca ttgtggatga atctcatatg acagtaccgc
      601 agatacgcgg gatgtacgcc ggcgaccagg cacgtaagaa ttcacttgtg gagtacggct
      661 tccgtcttcc gtccgcaaag gacaatcgcc ccttgaactt tgaggagttt gagggtaaaa
      721 tagaccagat gatgtttgtg tcggcaacgc cggcagaata tgaaaaaaca catgagctgc
      781 tgcgggctga acagatcatc agaccaaccg gactgctgga ccctgaaata tcggtgaggc
      841 cgacgcaggg acagatcgac gatctgatcg gcgagatcaa taaagaaaca gccggtaaaa
      901 ataaaatcat ggtaacgacg ctgacaaaac ggatggctga ggatctgacg gagtatatgc
      961 gggaggttgg catccgtgtg aaatatcttc attcggatgt ggatacactg gagcgtgccg
     1021 aaattatccg tgacatgcgt ctggatgtat tcgatgtact gatcggtatc aatcttctgc
     1081 gtgaaggtct ggatatcccg gagatcacac ttgtggcgat tctggatgcg gataaggagg
     1141 gttttctgcg ttcggaaaca tccctgatcc agacggtcgg ccgggctgca aggaatgcgg
     1201 acggacacgt gatcatgtat gcggatacga tgaccgattc catgcggcat gcgatcgatg
     1261 aaacgaacag acgccgaaag ctgcagcagg cttataacga agaacatggt attacaccac
     1321 agacgattaa aaaggcggtt cgcgatctga tcagtatttc cagagaaatt gccaaagagg
     1381 agatacgttt tgagaaggat cccgaatcca tgaaccggaa agagctggag aagctgattg
     1441 agcagatctc aaagaagatg aagaaagctg cggctgagct ggatttcgaa acggcggcag
     1501 aactgaggga taagatgatc agtctgaaaa agcagctgaa ggaatagaag agggcattac
     1561 gggcagagag ccaatagacg gaggatatgc ggtgaataag atttcgattg tagtaccaac
     1621 ctataacgag tcggaaaatg taggaaatct ggcgaaccag atcgactatg cattaaagag
     1681 catcccctat gaaattattt ttgtggatga cagtacggac catacaccgg aagtaataaa
     1741 aagggtgcag gcggaaaatg caaatgtacg gatggaacac agggagagtg aaaaaggact
     1801 tgccacggca gtactgaagg gattttccct ggcctccggc gattacattg ccgttatgga
     1861 tgcagatctg cagcatccgc cgtcaatttt gcgcgcaatg tatgcggtca tggaaagcgg
     1921 tgcggatttc tgtgtaccga gccgtttcat tcccggcggc agtgacggcg gccttggtcc
     1981 atatagaaaa cttgtttccg gcgtggccag atatattgga aagatactgc ttccgtgcct
     2041 gcgtaagatc acagatccga caagcggtct ttttatgata aaaaagaagg ttatcaggga
     2101 ctgtgacctg cagccgatcg gctggaaaat acttgtagag gtgctggcaa tggggacata
     2161 tgggaatgtc gtggagatcc cctataaatt tcaggcaaga ccggccggag aatcaaagct
     2221 ttcttccaaa gtaacggccg aatatttaaa acaggtgtgc ggtcttatga aacgcggaaa
     2281 aaaacagaaa caggtcatgg tctccagatg gacgcaggag atgataaaga cagcggagaa
     2341 gaaatatgat atttagttca gttttatttt tatgtatttt tctgccggtt atgattatcg
     2401 gttattatgc agttccaaag aaattcaaga atgcttacat gcttcttgga agtctgtttt
     2461 tttatacgtg gggagagcca aagtatattt ttcttattat tgcttccgtt atcggcaatt
     2521 atttttttgg acttgcgatc tatcgtcttg caaaaaaaca ggaagagaaa aagtttgttt
     2581 atgcaaaaaa agcggtactg atcgtttcgc ttttatgtaa tctgggcgtt cttgtttatt
     2641 tcaaatattt tgtgctgctt gtttcaacag ctgacgaact gctccatgca acgctgacga
     2701 ttccggagat cgtgcttccg atcggaattt cattttatac gtttaaaagt atatcctacg
     2761 tggtagatgt ctaccgtacg gaagggaaaa aagacgaaga cggaaaaaca cttacacttg
     2821 tagagaaaaa tccgatcgat ctggctcttt atatatccat gtttccggaa atcctgtccg
     2881 gaccgatcag ccggtataag gatgtaaagg attccctaaa agagccgaag ctgagtggtg
     2941 cggctttttc ttccggtatt gaacgcttta ttgtcggcct tgcaaaaaag gcgatcattg
     3001 caaacagcat cggtgaggtt gcggataaga tctttgcgat ggatctaagc tatgcaggga
     3061 cgctggcggc atggcttggg gcaattctct atacgcttca gatctattat gatttttccg
     3121 gatattccga tatggcgatc ggccttggga aaatttttgg ttatgatctc atggaaaact
     3181 tcaattatcc gtacatttcc aaatctatta cggaattctg gcgcagatgg catatttccc
     3241 tttcgacatg gtttcgggac tatttataca ttccgctcgg aggaaaccga agaggaaatg
     3301 tttatttcaa tttatttgtt gtttttcttg ctaccggtat ctggcatggg gctgcatggg
     3361 gctttttgat atggggtctg tggcatggtc tttttatgct gatcgaacgc gttttcagaa
     3421 acaaaaaaat aaaattacag attcccggtt ttatcaaatg ggtttatgca atgcttgtag
     3481 ttgtattcgg ctgggtattg ttcaaactgg aagatatcaa tacggccctg tcctatatcg
     3541 gcgtgatgtt ccatacaaag aagtattcat atgttgcgtt ttcgcttcgt tattatctgg
     3601 ataacaagat gatttttatt ctgatcgcgg ctgtccttgc ggctgtgccg tgggcacagg
     3661 tgcttccgag gaattttggt tccgtaattg cggcagcgtc tattgcaaaa ccggcgaaac
     3721 cggcatgtat tgtgaaacgc atactgctga tcgcgctgct cctgctcagt atgatcttta
     3781 tcgtaaacag cacatataat ccatttattt attttaagtt ctgacgcatt gtgaaaggaa
     3841 tatgtgatga aaaacaaaat agcaaacggt atttttataa tt
//
LOCUS       DAOSYE010000086         3880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16356_length_3880_cov_3.012026, whole genome shotgun sequence.
ACCESSION   DAOSYE010000086 DAOSYE010000000
VERSION     DAOSYE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16356_length_3880_cov_3.012026"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..130
                     /locus_tag="PLU43_02320"
     tRNA            60..130
                     /locus_tag="PLU43_02320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Gly,seq:tcc)
     gene            199..270
                     /locus_tag="PLU43_02325"
     tRNA            199..270
                     /locus_tag="PLU43_02325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Gly,seq:gcc)
     gene            364..705
                     /locus_tag="PLU43_02330"
     CDS             364..705
                     /locus_tag="PLU43_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO27273.1"
                     /translation="MTSEESLQYHMRLRIYRNEICFGPGLAELMELVKEKGSLSGAVK
                     EMNMAYSKAWKIIKRAEEDLGFLLMEGQSGGRHGGRTVLTEKGEELLDKYTGFVRESQ
                     KEVDKIFQKYF"
     gene            828..1748
                     /gene="modA"
                     /locus_tag="PLU43_02335"
     CDS             828..1748
                     /gene="modA"
                     /locus_tag="PLU43_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO27274.1"
                     /translation="MKKKLVSIMTAAVMTAVLLAGCGSTESADTTEDTAVQATETETE
                     PAAEETEEEAVIEETPETEEAVSEEASSEESISGELMIAAAASLENCLTTDVLPMFNE
                     KYPNITVTGSYDSSGKLQTQIEEGAPVDVFFSASTKQTTALTEEGLIEETDIVNLLEN
                     KIVLIVPEGSESEYSTFTDIVNADTIAVGDPESVPAGQYAQESLTNLGLWDEVLAKAS
                     LGTNVTQVLNWVAEGSADAGIVYATDAAQTDKVVVIDAAPADSVKAAVYPVALLKDCQ
                     NPETAKVFIEFLQSPEVIAVFEANGFTSAL"
     gene            1871..>3600
                     /locus_tag="PLU43_02340"
     CDS             1871..>3600
                     /locus_tag="PLU43_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOO27275.1"
                     /translation="MKVVLAAVNAKYIHSNLAVYSLKAYADQGLNGQADIVIREYTIN
                     QDREKVLADLYMQKPELLAFSCYIWNISYVRELAVQIKTLLPKTDLWLGGPEVSYEPE
                     KLFEELPIKGIMLGEGEAVFENLVSVYLRYEEQQREDALRRMKGIACRDFSTAPDTKL
                     LQMDELPFVYESFPEECFENRIVYYETSRGCPFCCAYCLSSIDKQVRFRSFSLVKREL
                     RFFLDRNVSQVKFVDRTFNCNPAHALEIWNYIKEHDNRITNFHFEIAADIMTDEELAL
                     IRTMRPGLIQLEIGVQTTNRETIRAVNRRMDFDKVISVVRRIQEGKNVHLHLDLIAGL
                     PKENFQSFRNSFNEVYALHPNQLQLGFLKVLKGTPIAENAAHDGIRYWTIPPYEVLFT
                     KELTFREVLQLKAVEEMVDLYYNSGQFCHTLRFLETYFGEAFAMYAALAGFYRERGYG
                     TQLPSRVRKYEILLEFAKDIPGVSSEKCGELLTLDLYLRENLKTRPFFSESQQPYKER
                     IRAFYKREEMNRQYLPEYAEFDFKQLMKLTHVEIFDLLADSRQVLLFNYKEKDALTNN
                     AKLITITHEL"
     assembly_gap    3601..3610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3625..>3880
                     /locus_tag="PLU43_02345"
     CDS             3625..>3880
                     /locus_tag="PLU43_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO27276.1"
                     /translation="MQKRTKEILEILDKQYSVEYKCYLNHEAPWQLLIATILSAQCTD
                     ARVNLVTKELFQKYPSVEAFAKADLKELEQDIHSTGFYHNK"
BASE COUNT         1107 a          740 c         1039 g          984 t
ORIGIN      
        1 gtagagcaga ggactgaaaa tcctcgtgtc actggttcga ttccggttct gggcactttg
       61 cgggtgtagt tcaatggtag aacactagcc ttccaagcta gatacgtggg ttcgattccc
      121 atcacccgct ttgtatatgt aacggggtgt cctgattgac aaaccaaatt gattttgtta
      181 cacttttaaa gtagttatgc acgagtggct cagtggtgga gtatcgcctt gccaaggcga
      241 gggtcgcggg ttcgaatccc gtctcgtgct cttttatgaa agttctgctg ttgatcttcg
      301 acgtcagggc ttttcgtttt atttacggaa attgcaggct gcaaaaaagg gacaggtgat
      361 gacatgacat cagaagaatc tctgcagtat catatgcgtt tgagaattta caggaatgaa
      421 atatgctttg gtcccggtct ggcagagctg atggagcttg tgaaggagaa gggctcgctg
      481 tcgggggccg tgaaggaaat gaatatggca tattccaagg catggaagat catcaaacga
      541 gcggaagagg atcttggctt tttgctgatg gaagggcaga gcggaggcag gcacggaggc
      601 aggacagttc ttacagaaaa aggggaagag cttcttgaca aatataccgg atttgtgaga
      661 gaatctcaga aagaagtaga caaaattttt caaaagtatt tttgatccgt gcagagtatg
      721 gcagagacga atggcattga taaatgatgc gaaagctgat ttatcatatg ccatttatat
      781 ataaaaccgt acatcttgta cgagaataac gcaaaggaga gtgcattatg aaaaagaaac
      841 tagtatctat tatgacagca gctgttatga cggctgtgtt gcttgcaggc tgtggttcga
      901 ctgaatcggc agatacgacg gaggatacgg ccgtacaggc aactgagacg gagacagaac
      961 cggcagcaga agagacggag gaggaggctg taattgagga aacgccggaa actgaagagg
     1021 cagtttctga ggaagcttct tcggaggagt caatttccgg agagctgatg attgcggcag
     1081 cggcaagcct ggaaaactgt ctgacgaccg atgttcttcc gatgtttaat gaaaagtatc
     1141 cgaatattac cgtaacaggc tcctatgaca gttccggaaa gctgcagaca cagatcgaag
     1201 agggagcgcc ggtcgatgta tttttctctg cttccacaaa gcagacgaca gcgcttacgg
     1261 aagaaggcct gatcgaggaa acggatatcg tgaatcttct ggaaaataaa atcgttctga
     1321 tcgtaccgga aggctcggaa tcggaataca gtacatttac ggatattgtg aatgcagata
     1381 cgattgcggt cggagatccg gaaagtgttc cggcgggcca gtatgcgcag gagtcactga
     1441 caaatcttgg cctctgggat gaggtgcttg cgaaagccag tcttggaacg aacgtaacac
     1501 aggtattgaa ctgggtagcc gaaggcagtg cggacgcggg aatcgtttat gcgacagatg
     1561 cagcacagac ggataaagtc gttgtgatcg atgcagcacc ggctgacagt gtaaaagctg
     1621 ccgtctatcc ggtagcgctt ctcaaggact gccagaatcc ggagaccgct aaggtattta
     1681 tcgaatttct gcagtcaccg gaagttattg ctgtatttga agcgaacgga tttacctctg
     1741 ctttatagtt ttataaaatt taatgaccgc agatacttca atttgcgaag tggtgtaaga
     1801 tatgatagaa taaagccgat ttattctatc atattttatg taaaaaataa acagattcgg
     1861 ggatgccgcc atgaaagtag tgcttgctgc ggtcaacgca aaatatatcc attccaatct
     1921 tgccgtatac agcttgaagg catatgcgga ccagggactt aatgggcagg cggatatcgt
     1981 aatacgggaa tatacgatca atcaggacag ggaaaaggtg cttgccgatc tttatatgca
     2041 gaagccggag ctgcttgcct tttcctgtta tatttggaat atttcctatg tccgggagct
     2101 ggcggtgcag atcaagacac tgcttcccaa aaccgatcta tggcttggcg gaccggaagt
     2161 gtcttatgaa ccggaaaagc tgtttgaaga gcttccgatc aaagggatca tgcttggaga
     2221 aggagaggct gtttttgaaa atcttgtttc ggtatattta agatatgagg agcagcagag
     2281 ggaggatgcc ttaaggcgta tgaaagggat cgcctgccgg gatttttcca cagcaccgga
     2341 tacaaagctg ctgcagatgg atgagctgcc gtttgtatat gagagctttc cggaagaatg
     2401 ctttgaaaac agaatcgttt attatgaaac gagccgcggg tgtccgttct gctgtgcgta
     2461 ctgtctgtct tctattgaca aacaggtccg ttttcgcagc ttttcgcttg taaaaagaga
     2521 gcttcgtttt tttctggaca gaaacgtttc tcaggtaaag tttgttgacc ggacatttaa
     2581 ctgcaatccg gcacatgcgc ttgagatctg gaattatatc aaagaacatg ataatcgcat
     2641 aacaaatttt cactttgaga tagcagcgga tattatgact gatgaagagc tcgcgctgat
     2701 ccgtacgatg cggcccgggc tgatacagct ggagatcggt gtacagacga caaacaggga
     2761 aacgatcagg gcagttaacc gccggatgga ttttgataaa gtgatttctg ttgtcaggcg
     2821 catacaggaa ggaaaaaatg tacatctgca tctggatctg atcgccggac ttccaaagga
     2881 gaatttccag agcttccgaa attcttttaa tgaagtatat gcgcttcatc cgaatcagct
     2941 gcagctcggg tttctgaagg tcttaaaggg aacaccgatc gcggaaaatg cagcgcatga
     3001 cgggatccga tactggacga taccgccgta tgaagtcctt tttacaaagg aacttacttt
     3061 tcgggaagta ttgcagctga aagcagtgga ggaaatggtg gacttatatt ataacagcgg
     3121 tcagttttgc cataccctcc ggtttttaga aacttatttt ggagaagcgt ttgcaatgta
     3181 tgcggcattg gccggctttt acagggaaag aggctatggt acgcagctgc cttcacgtgt
     3241 gaggaaatat gagatcctgc tggaatttgc caaagacatt cccggtgttt catctgagaa
     3301 atgcggagag cttctgacgc ttgaccttta tttacgggaa aacctgaaaa ccaggccctt
     3361 tttctcagaa tcgcagcagc cctataaaga acggatccgc gcattttata aaagagaaga
     3421 aatgaacagg cagtatctgc cggaatacgc ggagtttgat tttaaacagc tgatgaagct
     3481 gacacatgtt gaaatatttg atttgcttgc ggacagcagg caggtacttc tttttaatta
     3541 taaggaaaag gatgcattga caaataatgc aaagctgatc acgatcacac atgaactgga
     3601 nnnnnnnnnn agggacagac agacatgcag aaaagaacaa aggaaatact ggagattctt
     3661 gataaacagt attcggtcga atataaatgc tatctcaatc acgaggcgcc atggcagctt
     3721 ctgatcgcga cgatcctcag cgcgcagtgt acggatgcga gggtcaatct cgtaacaaag
     3781 gagctgtttc aaaaatatcc gtctgtcgaa gcgtttgcaa aagcggatct gaaagagctg
     3841 gagcaggata ttcattctac cgggttttac cataataaag
//
LOCUS       DAOSYE010000087         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16551_length_3848_cov_2.566570, whole genome shotgun sequence.
ACCESSION   DAOSYE010000087 DAOSYE010000000
VERSION     DAOSYE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16551_length_3848_cov_2.566570"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLU43_02350"
     CDS             complement(<1..182)
                     /locus_tag="PLU43_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27277.1"
                     /translation="MILAAESEKAAYVIGYDLNDMMSQISYYELNRNVPETLISDSEE
                     EKLGIPTVLCKRRKVS"
     gene            complement(197..3763)
                     /locus_tag="PLU43_02355"
     CDS             complement(197..3763)
                     /locus_tag="PLU43_02355"
                     /inference="COORDINATES: protein motif:HMM:NF040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HOO27278.1"
                     /translation="MSGLREIVDKLVNGFFEYDCGKLSFSVSKIEANLPAGEVYEGSF
                     MMKSRTGTLVKGNVYTSSMRLVCRTDYFEEVEKEVAFLFDPTGLEPGDVIKGDVQIVS
                     NAGEYYIPFVFSIVHSVVKSSLGSVKNLFHFTNQAQSNWEEAVQLFYSPEFMQVFEGN
                     DRIHLDKYRGLSNLKMDELCVDEFLVSINKKQPVVYSVDRSVYEFYDVTQELRCEIIL
                     RKSTWGYIHLDLKTDNAFLRMEKESLSDDDFLGNEHRLIFYIEEEQLHEGKNFGRLIL
                     SNTFQTLTVTVVAARRRHSSPAYFENREKKKLTCRLMRQYVAFRTKQISVGTWVRESM
                     KIVERMNALDDKDPVSRLYQAQLLLVEERYNEAKWILEHVENEMNIRQASSECYCYYL
                     YLTTLYQRKEEYVNQIASIVTDIFNKNKNSFRILWTLLYLDEELNRNSSRKIALIERQ
                     FEGRCFSPVLYIEAYNYYTANPVALNKLTDFEIQVLLFAVKSGKLDRDLQKQLIYLAG
                     RQKTFSDQLLKILMMSYKLARDEELVEVICMLLIKGEKTEARYFKWYARGVECGLRIT
                     RLYEYYIFSIPLSYRELLPKQVLMYFGFHNELDHVRLAFLYANLIRHKRMVPELYESQ
                     RENMYIFTIEQIEQEHMDANLAVIYEDILFGEMIKPDMANHLAKLLFACEVTAVCADT
                     KKIVVLQEEFCGEQSYPVENLYAYPYIYSQDSMVFYEDEQGKRSIVNESHVRKLMNET
                     LYIPSIKYYVNNNLFFSLYLCEGRRHYVVVEEENADLCRELVESGEIRENFKRDIRMG
                     LLHYYYDHDQITTLDEFLVNIDAKVLCTKDRAEVINCFVRRGMYEEAYELITIFGTEE
                     VSAKTCVKICSQMIDHKEQAPDVLLVKISYYAFRHGKYDTQTLKYLVDNFDGLTKELR
                     DLWKASRQFDIECYSLTEKLIIQMLYTRTTVGEKEEIFEYYLQRGASTKVELAYLSYN
                     AYDYFAKERMMDPRIFSYIVNNYRIGESLNDACKLALLKYYAEEEKKYSERIKEMLII
                     FIKDFLHKNIYFKFFAAFVGIVPELSAFSDKTIIEYRTNPGYHVVLHYILEGQNSSED
                     IYHTEEMRNMFGSVFSKEFILFFGENLQYYITEEKGGQEQLTVSDSVSISDAVAEGND
                     SRYSLLNDMVVSKTLQDDDTLIKLMEEYVEADYFTEQIFTLL"
BASE COUNT         1078 a          975 c          666 g         1129 t
ORIGIN      
        1 tggctgactt tcctgcgctt gcataatacg gtcggtattc cgagcttttc ttcctcggaa
       61 tcagaaatca gtgtttccgg cacattccta ttcagttcat aatagctgat ctgcgacatc
      121 atatcattca gatcataccc gatcacatat gccgctttct cagattccgc tgccagtatc
      181 aaaataatcc tccgctctat aataatgtaa atatctgttc cgtaaaataa tccgcctcca
      241 catattcttc catcagtttg atcagtgtat cgtcatcctg cagtgtttta gacacgacca
      301 tatcattcag aagactgtag cgtgaatcat ttccctccgc gaccgcatcg gaaatgctga
      361 cggaatcgct gactgtcagc tgttcctgcc cgcccttttc ctccgtgata taatattgca
      421 gattttcccc gaaaaacagg atgaattcct tggaaaacac actgccgaac atgttgcgca
      481 tttcttccgt atgataaata tcctcagaag aattctgtcc ttccagaata taatgcagca
      541 ccacatggta gcccggattt gtccggtatt caataatcgt tttatcggaa aaagcggata
      601 actcaggaac aattccaaca aaggcggcaa aaaacttaaa ataaatattt ttatgaagaa
      661 aatccttaat gaatatgatc agcatctcct tgatccgttc actgtatttt ttttcttctt
      721 ccgcatagta ttttaacagc gccagtttgc acgcatcatt cagagactcc ccgatcctgt
      781 agttgttcac aatatacgaa aagatcctcg gatccatcat tcgttccttt gcaaaataat
      841 catatgcatt ataggacaga tacgcaagct ccaccttcgt actggctcca cgctgcagat
      901 aatattcaaa gatctcttct ttttctccaa cggtcgtacg tgtataaagc atctgaatga
      961 tcagcttttc cgtcagcgaa tagcattcga tatcgaactg tctggaagcc ttccacagat
     1021 cgcgcagctc ctttgtaagt ccgtcaaaat tatcaacaag atatttcagt gtctgcgtat
     1081 catacttacc gtgtctgaat gcataatacg atattttaac aagcagtaca tccggggcct
     1141 gttctttatg atctatcatc tgactgcata ttttcacaca cgtctttgcg gaaacctctt
     1201 ccgtaccgaa gatcgtaatc agttcataag cctcttcata catcccgcgc cttacaaagc
     1261 agttgataac ttccgcccgg tctttggtac ataatacctt ggcgtcaata ttaacaagga
     1321 attcatccag tgtcgtgatc tgatcatgat cataataata gtgcagaaga cccatacgga
     1381 tatcccgttt gaaattttcc cgtatttctc cggactctac aagctcccgg cacaggtctg
     1441 cgttttcttc ctcaacgaca acatagtgac gccttccttc acaaagatat aaggaaaaaa
     1501 acagattgtt gtttacataa tatttaatag aaggaatata taacgtctca ttcatcagtt
     1561 tgcggacatg gctctcgttc acgatcgatc tctttccctg ctcatcttcg taaaacacca
     1621 tgctgtcctg gctgtaaata tacggatatg catacaggtt ttccacagga taggactgtt
     1681 cgccgcaaaa ctcctcctgg agcacaacaa tttttttcgt gtccgcacaa acagccgtaa
     1741 cctcgcaggc aaacaaaagc tttgccaggt gattcgccat atcaggcttg atcatttccc
     1801 caaacaggat gtcttcatag atcactgcca gattcgcatc catatgctcc tgctcgatct
     1861 gctcgatcgt aaatatatac atattttcac gctgagattc gtaaagctcc ggaaccatgc
     1921 gtttatgcct gatcagattt gcataaagaa acgcaaggcg tacatgatcc agttcattgt
     1981 gaaaaccaaa gtacatcaaa acctgttttg gaagaagctc tctgtagctg agcggtatgg
     2041 agaatatata atattcatag agccttgtaa tacgcagacc gcattccaca ccgcgtgcat
     2101 accatttaaa atacctggcc tcggtctttt cccccttgat cagcagcatg cagatcactt
     2161 ccacaagctc ctcatcccgc gcaagcttat aggacatcat cagtatcttc aaaagctgat
     2221 cggaaaatgt tttttgtctg ccggcaagat aaatcagctg tttttgcaga tccctgtcca
     2281 gctttccgct cttgaccgca aagagcagca cctgtatctc aaagtccgtc agcttattca
     2341 atgctaccgg attggccgta taataattgt aggcctcaat atacagcaca ggactgaagc
     2401 atctgccttc aaactgccgc tcgatcaaag cgatctttct ggacgaatta cggttcagct
     2461 cctcgtccag ataaagcagt gtccataaga tccgaaaact gtttttattt ttattgaaaa
     2521 tatccgttac aatggatgca atttgattta catactcttc cttccgctga tagagagtcg
     2581 tcagatataa gtagtagcaa tagcattcgg aagatgcctg cctgatattc atctcgttct
     2641 ctacatgctc cagtatccac tttgcctcat tgtaacgttc ctcaacaagc aaaagctgtg
     2701 cctggtacag tctggatacc ggatccttgt catcaagcgc gttcatacgt tcaacgattt
     2761 tcatggattc cctgacccac gtaccgacac tgatctgttt tgtccgaaag gctacatact
     2821 gtctcattag ccggcatgtc agctttttct tttccctgtt ctcaaaatag gcagggctgc
     2881 tgtgtctgcg ccttgctgca acaacagtga ctgtcagcgt ctgaaaagta ttcgacaaaa
     2941 tcagacggcc gaaattcttt ccttcatgaa gctgttcctc ttcaatataa aagataagcc
     3001 tgtgttcatt cccaaggaaa tcgtcgtccg acaggctttc tttttccatg cgcagaaacg
     3061 cattatctgt tttcaggtca agatgaatgt atccccatgt ggattttcta agtatgatct
     3121 cacagcgaag ctcttgcgtc acatcataaa attcatagac cgaccgatcc acactgtaca
     3181 caacaggctg ctttttattg atggacacaa gaaattcatc cacacacagc tcatccattt
     3241 tcaaattgct cagacccctg tacttatcca ggtggatcct gtcgtttcct tcaaaaacct
     3301 gcatgaattc cggagaataa aaaagctgta ccgcttcttc ccagtttgac tgggcctgat
     3361 ttgtaaaatg aaataaattc ttcacactgc caagagaact tttcacaaca ctatgaacga
     3421 ttgaaaatac aaaagggata taatactcac cggcatttga tacgatctgt acatctccct
     3481 tgatcacatc tcccggttcc aaacctgtcg gatcaaacag aaatgccact tctttttcta
     3541 cttcttcaaa ataatcagtc cggcatacaa gccgcatgct ggacgtataa acatttccct
     3601 tgacaagcgt acctgtccgg ctcttcatca taaaagaacc ttcgtatacc tctcctgccg
     3661 gaaggtttgc ttcaatttta gatacagaaa aactgagttt cccgcaatca tactcgaaaa
     3721 acccattcac caatttgtct acaatttcac gcaagccact caccacactc tgtaaaacag
     3781 ctcccccatt ccaaatatga ctccggacag ctattgcttt ttagttaaaa ttcattataa
     3841 taaagtat
//
LOCUS       DAOSYE010000088         3841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16600_length_3841_cov_2.779186, whole genome shotgun sequence.
ACCESSION   DAOSYE010000088 DAOSYE010000000
VERSION     DAOSYE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_3841_cov_2.779186"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLU43_02360"
     CDS             <1..1288
                     /locus_tag="PLU43_02360"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOO27279.1"
                     /translation="DRITGLGNLAGLTNTIQGLIDQKVVHRYTILYMSAYKFQLISRK
                     YGYELGSQVMLQVSKKLQVFTFAQSKEQMIARLVNDNFVVIVHNSNLQAYLDYLSAME
                     VSFEYNAEMVKQKISFCVGIYKMKEDDKSVQLPIEYATAAYNLASQGGCSTIVYYDEK
                     IHNQFLKEKDMEARMTDALHDGEFLVYYQPKIELSTNRIVGAEALVRWLSNGRILPPA
                     EFIPIFEKNGFVCQIDFFVLESVCKNMRKWLDAGLDVVKISVNFSRMHLLNEQFTNKI
                     VAMLKEYRIPARYIEVEFTESADWDDSALLSVVLEELKNNGIATAMDDFGTGYSSLSL
                     LTNLSFDILKIDKSLLDEKNISERGRVVMNNIIHMVQDLDIDVIMEGVETLEQVEFLK
                     EIHCNMAQGYLFDQPLPIEKFEERLDNGNYDRVFRS"
     gene            1401..2420
                     /gene="hflK"
                     /locus_tag="PLU43_02365"
     CDS             1401..2420
                     /gene="hflK"
                     /locus_tag="PLU43_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOO27280.1"
                     /translation="MAFSEFNGFDQNGQFNAFHKKQKKEKKNSAGPSAGKVFKLVGIA
                     LLLLVVLGLVFSSFYTIQEEEQAVVCTLGVPKAVTEPGLHFKIPLIQTVDKVNTTIQG
                     FAIGYTSDYEYNYNGEEGAEEGMMITSDYNFIKIDFYAEYRITDPVKALYASEDPQLI
                     LTNIAQNCIRTTIGSYNVDAVLTTGKNEIQANIKEMILEKLESLDIGIQLVNITIQDA
                     EPPTDDVVTAFKSVETAKQGKETALNNANKYRNEQIPNAEAQADKILQDAEAEKQARI
                     NEAQGQVARFNSLYNEYVKYPTITKQRMFYETMEEVLPDMKVIIQGTDGSSMQEILPL
                     DSFTE"
     gene            2433..3458
                     /gene="hflC"
                     /locus_tag="PLU43_02370"
     CDS             2433..3458
                     /gene="hflC"
                     /locus_tag="PLU43_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOO27281.1"
                     /translation="MAEAERFHSDGTSFNDNNKKKKSRKTWLIWVVVIAALLLASSSL
                     VICHEDEFKLIRQFGRVDRTISESGMYFKIPFIESVDTIPKEILIYDLPASDVITSDK
                     KTMIIDSYVLWRVTDPLKFAQNLSYSITNAENRIDTLVYNAMKNTISNMKQDEVIKSR
                     DGKMTITNNEADDGETTSNDLVIEDNTEVVEIKSLTDEIMDHIGTIYDQYGIEILTVE
                     VKKLDLPDDNKQAVYTRMISERENIAAQYTAEGASQAQIIQNTTDKEVSIMLSESNAK
                     AEELKAEGEAEYMRILSDAYSDEEKSEFYTFVRSLDAAKASMQGSGEKTLILPADSPI
                     AEVFTQK"
     gene            3479..>3841
                     /locus_tag="PLU43_02375"
     CDS             3479..>3841
                     /locus_tag="PLU43_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27282.1"
                     /translation="MISEKMVELVKGSSVIRAMFEEGQKLAKIYGAENVYDFSLGNPN
                     VAPPAAVKEAIIDLAEHDNPMHLHGYMSNPGFEDVRETIAKSLNRRFGTAYGAENIIM
                     TVGAASGLNVIFRALLNPD"
BASE COUNT         1190 a          683 c         1004 g          964 t
ORIGIN      
        1 tgaccggatc acgggacttg ggaatcttgc cgggcttacg aatacgatac agggtctgat
       61 cgatcagaaa gttgtgcacc gctatacgat tctttatatg agtgcataca aatttcagct
      121 gatcagccgg aaatatgggt atgagcttgg aagtcaggta atgctgcagg tttcaaagaa
      181 gctgcaggtg tttacgtttg cacagtcaaa agaacagatg atcgcacgtc ttgtcaatga
      241 caattttgtt gtgatcgtac acaactccaa tctgcaggcc tatctggact atttatctgc
      301 catggaagtt tcgtttgaat acaatgcgga gatggtaaaa cagaaaattt ctttttgtgt
      361 cggcatctat aagatgaagg aagacgacaa aagtgtgcag cttcccattg aatatgcaac
      421 ggcagcttat aaccttgcca gtcagggagg atgctcgacc attgtttatt atgatgaaaa
      481 aattcataat cagtttctga aagaaaaaga catggaagcc agaatgacgg atgccttgca
      541 tgacggagaa tttttagtat attatcagcc taaaatagag ctcagcacaa accgtatcgt
      601 cggagccgaa gcgctcgtgc gttggctttc aaacggcagg atcctgccgc ctgcagagtt
      661 tattcctatt tttgaaaaga acgggtttgt gtgccagata gatttttttg tactggaatc
      721 tgtctgtaag aatatgagga aatggctgga tgccggcctg gatgttgtaa agatctcggt
      781 caatttttca aggatgcatc ttttgaatga acagtttacg aataagatcg ttgccatgct
      841 gaaggaatac agaattccgg ccaggtatat cgaagtggaa tttacggaaa gtgcggactg
      901 ggatgacagc gcgctgctgt ccgtggtgct tgaggagctg aagaataatg ggatcgcgac
      961 cgcgatggac gattttggaa cgggttattc ctctctaagc cttctgacga acctttcctt
     1021 tgatatttta aagatcgata aatcgcttct tgatgaaaag aacatatcgg agcgcggacg
     1081 ggttgtgatg aacaatatta ttcatatggt gcaggatctg gatattgatg tgattatgga
     1141 aggtgtggaa acactggagc aggtagagtt tctaaaagaa attcactgta atatggcaca
     1201 gggttatttg ttcgatcagc ctctgccgat agaaaaattc gaagagcgac tggataatgg
     1261 aaactatgac agagtttttc gttcataaaa atgaaaatat gatgaaaaag caaaaagtat
     1321 ttatcttttt gcttttttaa tgatataatg agtaggtgtc tggctgattt taaagggaaa
     1381 ataattaggg aggtaaaaag atggcattta gtgaatttaa cggatttgat caaaatggtc
     1441 aattcaatgc tttccataaa aaacagaaaa aggaaaagaa aaacagtgca ggtccttctg
     1501 cgggaaaagt gtttaagctg gtcggaattg cgctgctttt gctggtcgtg cttggactgg
     1561 tttttagttc tttttatacg attcaggaag aagagcaggc agttgtctgt acgcttggcg
     1621 tgccaaaggc cgtaacggag ccgggacttc atttcaagat tccgctcata cagaccgtgg
     1681 ataaagtaaa tacaacgatc caggggtttg cgatcggcta tacaagcgat tatgaatata
     1741 attataatgg cgaggaaggc gcagaagaag gaatgatgat cacctcagat tataatttta
     1801 tcaaaatcga tttttatgcg gagtaccgta ttacggatcc ggtcaaggca ctgtatgcat
     1861 ccgaggatcc gcagttgatt ctgacgaata tcgcacagaa ctgtattcgt acaacgatcg
     1921 gttcttacaa tgtagatgcg gttttaacga caggcaaaaa cgagattcag gccaatatta
     1981 aagaaatgat attggaaaag ctggaaagtc tggatatcgg tatccagctt gtgaatatta
     2041 cgatacagga tgcggagccg ccgacggatg acgtcgttac ggcatttaag tcggtggaaa
     2101 ctgcaaagca gggcaaagag accgcactga acaatgcgaa taaatacagg aacgagcaga
     2161 tcccgaatgc ggaggctcag gcggataaaa tactgcagga tgcggaggcc gaaaagcagg
     2221 cacgtatcaa tgaagcgcag ggacaggtcg ccagatttaa ttccctgtat aatgaatatg
     2281 taaaatatcc gacgattacg aaacagcgga tgttctatga aacgatggaa gaagtgctcc
     2341 cggatatgaa ggtcattatt cagggcacgg acggcagcag tatgcaggaa atactgccgc
     2401 ttgattcttt tacggaataa ggaggggaaa aaatggctga agcggaaaga tttcattcgg
     2461 acggaacatc gttcaatgat aataataaaa agaaaaaaag caggaaaaca tggctgatct
     2521 gggtagtcgt gatcgcagca ctgctgcttg cgagctcttc cctggttatc tgtcatgagg
     2581 atgaattcaa gctgatcagg cagttcggcc gtgtggaccg gacgatcagt gagtcgggaa
     2641 tgtattttaa gatcccgttt attgagtcgg tagatacgat tccaaaggag atcctgatct
     2701 atgatctgcc tgcgtcggat gtaattacgt cggataaaaa gactatgatc atagacagct
     2761 atgtgttatg gcgtgtaaca gatccgctga aatttgcaca aaatctgagc tactcgatta
     2821 caaatgcgga aaaccgcatt gatacactcg tatataatgc aatgaaaaat acaatatcaa
     2881 atatgaagca ggacgaggtc atcaagagcc gtgacgggaa aatgacgatt acaaacaatg
     2941 aagcagacga tggagaaaca acatccaacg atctggtgat cgaggacaat acggaagtcg
     3001 tggagatcaa atcgctgaca gatgagatca tggaccatat cggaacgatc tatgatcagt
     3061 atgggatcga gatactgacc gtggaagtaa agaaactgga tctgccggac gacaataagc
     3121 aggctgttta tacacgtatg atttcggagc gtgaaaatat tgccgcgcag tatacggcag
     3181 aaggtgcatc acaggcacag atcattcaga atacgacgga taaggaagta tccattatgc
     3241 tttccgagtc aaatgccaag gcagaagagc tgaaagcaga aggggaggcc gagtatatga
     3301 ggatcctctc agatgcgtat tccgatgagg aaaaaagtga attttatacg ttcgtgcgtt
     3361 ctcttgatgc ggcgaaggca agcatgcagg gttccggtga gaagacactg atactgccgg
     3421 ccgattcacc gatcgcggaa gtatttacac aaaaataaaa agaagagagg atttttatat
     3481 gatttcggaa aaaatggtgg aactggtaaa gggaagttcg gtcatcaggg cgatgtttga
     3541 ggaaggacag aagctggcaa agatctacgg agctgagaat gtatatgact tcagcctggg
     3601 caatccgaat gtggcgccgc cggcggccgt aaaggaagcg atcattgatc ttgccgagca
     3661 cgacaatccg atgcacctgc acggatatat gagcaatccc ggctttgaag atgtccgtga
     3721 aacgatcgca aagtctttaa acagacgttt tggcactgct tatggtgcgg aaaatataat
     3781 tatgacagtc ggtgcggcca gcggtctgaa cgttattttc cgcgcgctgc tgaatccgga
     3841 t
//
LOCUS       DAOSYE010000089         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_16619_length_3837_cov_4.071920, whole genome shotgun sequence.
ACCESSION   DAOSYE010000089 DAOSYE010000000
VERSION     DAOSYE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16619_length_3837_cov_4.071920"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PLU43_02380"
     CDS             complement(<1..1398)
                     /locus_tag="PLU43_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27283.1"
                     /translation="MKKKLLSVILAVSMTAALLAGCGSSEEAAPAADQPAADEATEET
                     AQEPADEATEEVAESTDTTDSTEATGEDPQLEKTIKILSIWAEDNDNGVLINEICNAY
                     KEVNPNFNFEYEYVSSDDLKQKVATLAASNDLPDLFVYESGKPILDLVDADQIMDVGE
                     ILDEYGCADKLSPSAVSLLKTLSGTEQLYDLPLGLNVEGFWYNKALFEQAGVEVPTTW
                     DEFEDVLKTLNDAGIQPLSCGAADKWGATRIMNAYLVRTAGADAMAKAADGEASYTDA
                     AYVAAAQKLQDWANEGYFGKGVTTVDMNTAGTMLMTGEAAIFYNGSWFTQNLTDTTAN
                     LAGEDGIGFFNVPVVDASISDAESYSMNCGNILCVGKEKYDEATGWFLKYFVENIGNV
                     AMEEQGTVKGYTYDVEPKNNSGYTQLVLDEIDKATSAFTWYEATMGSEVSTVAQENVQ
                     TLLNGDMTAEEYMQSI"
     gene            complement(1566..2609)
                     /locus_tag="PLU43_02385"
     CDS             complement(1566..2609)
                     /locus_tag="PLU43_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27284.1"
                     /translation="MYRMVVVDDEYIVVEGIKTILEREKMNCEIVGFAYDGIEAIHVI
                     KETTPDIVITDIRMPGMDGLSLIEECREFLPEASYVIISGYTEFEYARRALVLDVMNY
                     IDKPITIEKIADVVEQLDKKRQPDSKSEARKQLNLAIDDMVEAAMSRDPALFTEAAKK
                     LPALFEKATISFEQYKQEIFKTMAMLAEIFNEHNKRKDKLFQVSYQEAEQWKDKEEAE
                     EYMNRLFGQLAGTIQGERTGSAHRTVQHLLQYIEENYAENIGLNELADMVGLNPTYLS
                     ILFKEEVGVSFVKYMTELRVEKAKELLDAGYRVGEVSDMVGYSNYRYFCDIFKKHEGK
                     TPNEYRGCIRKQI"
     gene            complement(2584..>3837)
                     /locus_tag="PLU43_02390"
     CDS             complement(2584..>3837)
                     /locus_tag="PLU43_02390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO27285.1"
                     /translation="NVLFDDKNMTFSMVKEIISPSTGDFLGYLIVNVKKEILDEAFGE
                     MDEDYTTNRYLIVRRTDQISQENIEDRIVYFNGKEDDKQVIVNDYLMFGNDTKYLFSS
                     VQNDISGWEIVNVIEKDELSRDSSYIGWIALLIGILMLSVCVLLSTLISRAISKPLVM
                     LESTIKDVGNGNYRVEAEFDESEIGRIGSQFKHMVNNNLELHERLLNSEIKEKEAELL
                     LLQSQINPHFLYNTLDALYFMAVIDQADDVAEMVLALSDTFKLSLNKGDKLIFVRDEI
                     AKIKAYMKVQNMRHHNRFQFQVELDDEMLSEKMLTFILQPLVENAVYHGLEPRIGSGY
                     IRLEGYMEKEEMIFHIRDNGVGIDDLSKLEDGYGVKNIRERIKLFYGEEYDVIFDSRP
                     GEGTTVTIILPVIKEESYVSDGCRR"
BASE COUNT          950 a         1024 c          641 g         1222 t
ORIGIN      
        1 aatagactgc atatattctt ccgccgtcat atcaccattc agcaatgtct gcacattttc
       61 ctgtgcaact gtagatactt cactgcccat cgttgcttca taccatgtaa atgctgatgt
      121 agctttatcg atttcatcaa gtacgagctg cgtataaccg gaattgttct taggctctac
      181 gtcatatgtg tagcctttca ctgttccctg ctcttccatt gctacatttc ctatattctc
      241 gacaaaatat tttaagaacc agccggttgc ttcatcatat ttttccttgc cgacacataa
      301 aatgttgccg cagttcatcg aatagctttc agcatccgaa atagatgcat caacaaccgg
      361 aacattaaag aaaccgattc cgtcttcacc tgcaagattt gccgttgtat ctgtcagatt
      421 ctgtgtgaac caggagccgt tatagaaaat ggcagcttca ccggtcataa gcatcgtacc
      481 tgccgtattc atatcaacgg tcgtaacacc tttgccgaaa tagccttcat ttgcccaatc
      541 ctgcagcttc tgtgctgctg ccacataagc ggcatccgta tagcttgctt ccccatcggc
      601 tgcttttgcc atggcatcgg cgcctgctgt tctgacaaga tatgcattca tgatacgtgt
      661 tgcaccccat ttatcggctg ctccgcagga aagcggctgg atgcctgcat cattcagtgt
      721 cttaagaaca tcttcaaatt catcccatgt tgtaggaact tcgactccgg cctgctcgaa
      781 taatgcctta ttataccaga atccttccac attcagccca agcggcagat cataaagctg
      841 ttctgttcct gacaaggtct ttaataacga tactgccgaa ggactcagtt tatccgcaca
      901 tccatactca tccaaaattt caccgacatc catgatctga tccgcatcaa caagatcaag
      961 gatcggcttt ccggattcat aaacaaaaag atcaggcaga tcgttcgatg ctgcaagtgt
     1021 tgccactttc tgtttcagat catcggaaga aacatattca tattcaaagt tgaaatttgg
     1081 atttacttcc ttgtaagcat tgcagatctc attgatcaaa acaccgttat cgttatcttc
     1141 tgcccagatc gataagatct tgatcgtttt ttcaagctga ggatcttcac ccgtagcttc
     1201 tgtagaatct gtggtatctg ttgactccgc cacctcttcg gttgcttcat ctgccggttc
     1261 ctgcgccgtc tcttcggtcg cttcatctgc tgccggctgg tctgctgccg gggctgcttc
     1321 ttctgaactt ccgcagcctg caagcaatgc tgcagtcatg gaaactgcaa gaataactga
     1381 taataacttc ttcttcattt caaaacctcc ctaattcaat ttttacggtg acaaacaatg
     1441 atctgtctac atttatagac tagcataggc attccatgaa ctctaggcgg taatttctta
     1501 taaattgtac acttttttta gaatttcatt ctttctattg tctgtttttt tgtattatcc
     1561 gttttttata tctgtttcct tatgcatcct ctatattcat tcggtgtttt tccctcatgt
     1621 tttttaaaaa tatcacaaaa ataccgataa ttactgtacc caaccatatc gcttacctct
     1681 ccgacacgat atccggcatc gagcagttct tttgcttttt caacacgaag ttccgtcata
     1741 tatttcacaa aacttacacc gacctcttct ttaaacaaaa tactcagata agtcggattc
     1801 agtcctacca tatccgccag ttcatttaat ccgatatttt ccgcataatt ctcttcaata
     1861 tattgcagca gatgctgcac ggtccggtgc gcactgccgg tccgttcgcc ctgaatcgtc
     1921 cctgccagct gcccgaacag ccggttcata tattcttccg cttcttcttt atccttccac
     1981 tgctcggctt cctgatagga gacctgaaac agcttatcct tccgcttatt gtgttcattg
     2041 aaaatttcag caagcatcgc catcgttttg aatatttcct gcttatattg ctcaaagctt
     2101 attgttgcct tctcaaaaag tgccggcagt ttttttgccg cctcggtaaa caaagccgga
     2161 tcccttgaca ttgccgcctc caccatatca tctattgcaa gattcagctg ttttcttgct
     2221 tcacttttac tgtccggctg ccgtttttta tcaagctgtt cgaccacatc agcaattttt
     2281 tctatcgtta tcggtttgtc gatataattc atgacatcaa ggacgagcgc acgccttgca
     2341 tactcaaact ccgtataacc gctgataatt acataggatg cctccggcag gaattcccgg
     2401 cactcttcga tcagcgacag accatccatt ccgggcatgc ggatatccgt gatcacgata
     2461 tccggcgtcg tttctttgat tacatgtatt gcttcgattc cgtcataagc aaagccaact
     2521 atttcacagt tcatcttttc ccgttccagt attgttttta ttccttccac cacaatatat
     2581 tcatcatcta cgacaaccat ccgatacata cgattcctcc ttaataaccg gcagaataat
     2641 tgttaccgtt gttccttctc ctggcctgga atcaaatatc acatcatatt cctcgccata
     2701 aaacaatttg atcctctctc tgatattttt tactccataa ccgtcctcca gctttgacag
     2761 atcgtcaatt ccaaccccat tgtctctgat atgaaaaatc atttcctcct tttccatata
     2821 cccttccagc cggatgtatc cgctcccgat cctcggctcc agtccatggt agaccgcatt
     2881 ttccacaagc ggctgcaaaa taaatgtcag cattttttcc gacagcatct cgtcatcgag
     2941 ctccacctga aactgaaaac gattatgatg gcgcatattc tgtaccttca tgtaagcttt
     3001 tattttggcg atctcatccc tgacaaagat cagcttatcc cctttattta agcttagctt
     3061 gaaggtgtcc gacagcgcca gcaccatttc cgccacatcg tccgcctgat cgatcactgc
     3121 cataaaatag agcgcatcca atgtattata taaaaaatgc ggattgatct gtgactgcag
     3181 caaaagcagc tctgcttcct tttccttgat ctccgaattc agcaggcgtt catgcagttc
     3241 caggttattg tttaccatgt gcttgaactg acttccgatc cttcctattt cactttcatc
     3301 aaattccgct tccacacgat aatttccgtt ccccacatct ttgatcgtcg attccagcat
     3361 gaccaaaggc ttgctgatcg ccctggatat cagcgttgaa agcagcacac atacggacag
     3421 catcaatatt ccgatcagca gggcgatcca gccgatatag ctgctgtcac ggctcagttc
     3481 atctttttcg atcacattta cgatctccca gccgctgata tcgttctgca cagaactgaa
     3541 caaatacttt gtatcatttc caaacatcag ataatcattc acgattacct gcttatcatc
     3601 ctcttttccg ttaaaatata cgatacggtc ttcgatattt tcctgactga tctggtctgt
     3661 ccgcctgacg atcaaatacc ggttcgtcgt ataatcctca tccatttcgc caaatgcttc
     3721 atccagaatc tcttttttga cattaacgat caaatatccg agaaagtcac cggtacttgg
     3781 tgatatgatc tcctttacca tggaaaaggt catatttttg tcatcaaaaa gaacatt
//
LOCUS       DAOSYE010000090         3770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17078_length_3770_cov_3.025841, whole genome shotgun sequence.
ACCESSION   DAOSYE010000090 DAOSYE010000000
VERSION     DAOSYE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17078_length_3770_cov_3.025841"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="pstA"
                     /locus_tag="PLU43_02395"
     CDS             <1..623
                     /gene="pstA"
                     /locus_tag="PLU43_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOO27286.1"
                     /translation="IGAAIYLNEYARPGRFVRIIEFTTETLSGIPSIIFGLFGMIFFG
                     SVFGLDYSLLTGAFTMTIMILPLITRNTQEALKTVPDTYRSGALGIGATKWYMIRTIM
                     LPSAMPGIVTGVILAIGRIVGESAALLFTAGSGFLLPKSGLGLLEKVTQPGGTLTIQL
                     YLCMSKAQYDEAFGIALILLIIVLIINFLTKYSVKKLDVNKGKNKE"
     gene            635..1402
                     /gene="pstB"
                     /locus_tag="PLU43_02400"
     CDS             635..1402
                     /gene="pstB"
                     /locus_tag="PLU43_02400"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOO27287.1"
                     /translation="MEEMAVNKISIKNLNLYYGENHALKDIDMEIKEKAITAFVGPSG
                     CGKSTFLKTINRMNDLIDSVRIEGEVSLDGENIYDPRVDTTLLRKKIGMVFQQPNPFP
                     MSIYDNIAYGPRIHGIKNKEELDEIIEESLRGAAIFDEVKDRLKKSALGLSGGQQQRL
                     CIARALAVKPDVLLMDEPTSALDPISTLKIEDLMQELKQKYTVAIVTHNMQQAARVSD
                     YTAFFLLGEVVEFDDTDVIFSHPRDKRTEDYITGRFG"
     gene            1480..2139
                     /gene="phoU"
                     /locus_tag="PLU43_02405"
     CDS             1480..2139
                     /gene="phoU"
                     /locus_tag="PLU43_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOO27288.1"
                     /translation="MTRFNFEYELDELHKNMEEMGNRVEQAIDDAIYALEKQDTALAK
                     RIVQNDRLIDDMEKSIEAKCLSLITKEQPVARDLRVVSATLKAVTDIERIGDHASEVA
                     ELVIRNGDSNLYVISDMMPQLVKEAKKMVHRSVSAFMKREIEEAREVIASDDGVDDLF
                     NQVKETIVQSFKDEFINPDMLVDLLMIAKYMERIGDHAVNICEWEIFRETGAVDNTRL
                     L"
     gene            2155..2856
                     /locus_tag="PLU43_02410"
     CDS             2155..2856
                     /locus_tag="PLU43_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO27289.1"
                     /translation="MAKIFVIEDDENISNLIKMVLEGYGYEVFLFEEAESALDMIEII
                     IPDLAIFDLMLPGIDGLTAIEKIRQMDRVKDIPMLILTAKDKEYDKVLGLDKGADDYM
                     IKPFGVMELAARVRSLLRRSARHEENHRVENEKIQIQELVIDTATREVTVNGEPVELT
                     YKEFELLLYLIENNKRVVSREELLNHLWGYECDVETRTVDIHIGTLRKKLKEIGTKYI
                     KTLRNVGYRFVMPEA"
     gene            2858..>3770
                     /locus_tag="PLU43_02415"
     CDS             2858..>3770
                     /locus_tag="PLU43_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO27290.1"
                     /translation="MKKAIRNRFLLILAFALIVSVVISYLTASSRLLENTIDGMLKTL
                     RAIDYSLDYDEDLQTQANQMKTSILGSNAHITVIQTDGTVVADTDQTDTASMENHIGR
                     EEIQEALQDGTGSSIRYSQTLHEQMLYTAMMAKDSRFVLRLSVRYTGARDYIRSLLPA
                     YIIATLLVLAISMVISMRFSDALTRPLKEITDEMSAAQGDIPEFHFKTYQYPELNRIS
                     YTTDKLTNEIKEYVEKVNFEKQIRQEFFSNASHELKTPITSIKGYAEILQNGFAKTTE
                     AKDDCIRRIIKESDHMTNLINDILMISK"
BASE COUNT         1167 a          656 c          964 g          983 t
ORIGIN      
        1 gcattggggc agctatttat ctgaatgagt atgccagacc agggcgtttt gtcagaatta
       61 ttgagtttac gacagaaacg ctttccggta tcccatctat tatattcggc cttttcggaa
      121 tgattttttt tggttcggta ttcggacttg attattcatt gctgaccggt gcgtttacga
      181 tgacgatcat gatcctgccg ctgattacaa gaaatacaca ggaggcattg aaaacggtgc
      241 cggatacata cagaagcgga gcgcttggga tcggtgcaac aaaatggtat atgattcgta
      301 caattatgct tccatcggcg atgccgggaa ttgtaacggg tgtcattctt gcaatcggca
      361 gaattgtcgg agaatcggca gcacttttgt ttacggcagg cagcggattc ctgcttccga
      421 aatcaggtct cggtctgctt gaaaaagtta cgcagccagg cggcacattg acgatccagt
      481 tatatttatg tatgtcaaaa gcacaatatg acgaagcctt tggtattgcg cttattttgc
      541 tgatcattgt tttgatcatc aattttctga caaaatacag cgtaaagaaa ctggatgtaa
      601 acaaagggaa aaataaagag tgaaagaaaa gcagatggag gaaatggctg tgaataagat
      661 cagcataaaa aatttaaatt tatactatgg agaaaatcat gctctgaaag atattgatat
      721 ggaaataaaa gaaaaggcaa ttactgcttt tgtcggtcca agcggatgcg gaaaatccac
      781 atttttaaag acaatcaaca gaatgaatga tctgatcgac agtgtcagaa tagaagggga
      841 ggtcagtctg gacggagaaa acatttatga tccccgtgtg gatacgacac tgcttcgaaa
      901 aaagatcggt atggtctttc agcagccgaa tccgtttccg atgagtattt atgacaatat
      961 tgcatacgga ccccgtattc atggaatcaa aaataaggaa gagcttgatg aaatcataga
     1021 agaaagctta aggggcgccg ctatttttga cgaagtaaag gacaggctga aaaaatcggc
     1081 gcttggtctt tcgggcgggc agcagcagag gctgtgtatt gcacgtgcgc ttgcggtaaa
     1141 gccggatgta cttttgatgg atgaaccgac ttcggcactg gatccgattt ccacgctgaa
     1201 gatcgaggac ctgatgcagg aattaaaaca gaaatatacg gttgcgatcg taacgcataa
     1261 tatgcagcag gcggcgcgtg tgtctgatta tacggcgttt ttcctgcttg gagaagttgt
     1321 tgagtttgac gataccgatg tgattttctc acatccacgg gataaacgta cggaagatta
     1381 tattacggga agatttggct gatcatcgat ttttttaaaa aatttaagaa ggatacttgt
     1441 ctgttttgta aagaataggt atgatagaaa ggaagaaaga tgacaagatt taattttgaa
     1501 tatgaactgg atgagcttca taaaaatatg gaggaaatgg ggaatcgtgt tgaacaggcg
     1561 atcgacgacg cgatctatgc cctggaaaag caggatacgg cactggcaaa acgtattgta
     1621 cagaatgacc ggctgattga cgatatggaa aaatcaatag aagccaagtg tctttccctt
     1681 attacaaagg agcagcccgt agcaagggat ctgcgtgtcg tgtcggcaac gctgaaagct
     1741 gttacggata ttgaaaggat cggggatcat gcttcggaag tggctgagct tgtgatcaga
     1801 aacggagaca gcaatttgta cgtgatctct gatatgatgc cgcagctcgt aaaagaagcg
     1861 aaaaaaatgg tacatcgttc cgtatcggca tttatgaaac gtgaaattga agaagcacgt
     1921 gaggtgatcg cttcggatga tggggtagat gatctgttca atcaggtaaa agaaacgatc
     1981 gttcagagtt ttaaagacga atttatcaat ccggacatgc ttgtcgatct tttaatgatt
     2041 gcaaagtata tggagcgtat cggcgatcat gcggtcaata tttgcgagtg ggaaattttc
     2101 agggagaccg gagctgtaga taatacaaga ctgttatagt gaggagtaat caaagtggca
     2161 aagatatttg taatagagga tgatgagaat atcagtaatt taatcaagat ggttttagaa
     2221 ggctacggat atgaagtctt tttatttgag gaggctgaat ctgcgcttga catgatagag
     2281 atcattattc ctgatcttgc tatttttgat ctgatgcttc cggggataga cggcctgacc
     2341 gctattgaaa aaatcaggca gatggatcgg gtaaaagata ttccgatgct gattttgacc
     2401 gcgaaagata aagaatatga taaggttctt ggacttgaca agggtgcgga cgactatatg
     2461 atcaaaccgt ttggagtcat ggaacttgct gcacgggtac gttcgctttt aagacgaagc
     2521 gcccgtcatg aggaaaacca cagagttgaa aatgaaaaga ttcagataca ggagcttgta
     2581 atcgatacgg cgacaaggga agtgaccgtg aatggggaac ctgtggagct tacctataag
     2641 gaattcgaac tgcttcttta tctgattgaa aataataagc gggtcgtatc cagagaggag
     2701 ctgctgaacc atttatgggg atatgaatgc gacgtggaaa cgcggactgt cgatattcat
     2761 atcggaacac ttcggaagaa gcttaaggaa atcgggacca agtacattaa gacattgcgg
     2821 aatgtcggtt accgctttgt gatgccggag gcttgatatg aaaaaggcaa ttagaaaccg
     2881 ttttttactg atacttgcat ttgcactgat tgtcagtgta gtgatttcct atctgactgc
     2941 cagcagccgt cttttggaaa atacgataga cggaatgtta aaaacacttc gggcgatcga
     3001 ttattcactg gattatgatg aagatctgca gacgcaggcc aatcagatga aaacgagcat
     3061 attaggttcg aatgcacaca ttacggtaat acagacagac ggaaccgtag ttgcggatac
     3121 ggatcagaca gatacggcat ccatggaaaa tcatattgga cgggaagaga tacaggaggc
     3181 gctgcaggac ggaaccggca gcagtatcag atattcccag acgctgcatg aacagatgct
     3241 ttatacggcg atgatggcaa aagacagcag gtttgttctg cggctgtctg ttcggtatac
     3301 cggagccaga gattatattc gttccctgct gcctgcatat attattgcta cgctgctcgt
     3361 acttgcaatt tccatggtga tctccatgcg cttctccgac gcgctgacaa gaccgttaaa
     3421 agaaatcacc gatgaaatgt cggctgccca gggggatata ccggaatttc attttaagac
     3481 ttatcagtat ccggaactaa acaggatttc ctatacgacg gataagctga caaatgaaat
     3541 caaggaatat gtggaaaaag tgaactttga gaaacagatc cggcaggaat ttttttcaaa
     3601 tgcatcccat gaattaaaga caccgatcac atcgatcaaa gggtatgcgg aaatattgca
     3661 gaacggattt gcaaagacga cggaagcaaa ggacgactgc atcagacgga tcattaaaga
     3721 atcggatcat atgacaaatc tgatcaatga tattctgatg atttctaagc
//
LOCUS       DAOSYE010000091         3767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17094_length_3767_cov_2.867996, whole genome shotgun sequence.
ACCESSION   DAOSYE010000091 DAOSYE010000000
VERSION     DAOSYE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17094_length_3767_cov_2.867996"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLU43_02420"
     CDS             complement(<1..1225)
                     /locus_tag="PLU43_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27291.1"
                     /translation="MKRENQKKNKILKRIAVISAAAAILAVSMAGAVVCGFFDRMHTG
                     TTEDADTLQQTEETTDPLQLMTEVVKQKEAGADGGKSTFTILEIVPDTTYAMIGYLIS
                     GKEPIGTFGMDALSNSITGSTNGRYTTYLTTPHIGNAGDVRAVVRSWGTYTGYFEKVT
                     SGEGLYAISTSESTEYDYLPVTAGDGEFKATIKYYVPKDDRPTEDTWEHVLLKYTVNN
                     YDISFKNQTTQTSNTVYYKVDTVTENKGSGYYSFDDTEQIYVYDTTGKSDYDVTFVTT
                     DWNTSGVKNYVVDAISYNPKEGAYCAYIDPDQWIEKTAAVTDTTDYNYQYAISNICMV
                     SKLCKDNFSNTGNFDYVWHDDNSLSLECTTESDFEDGGNIYLYDYLKSKTINNEMFKL
                     FCLAIDGADGENGIHT"
     gene            complement(1236..1913)
                     /locus_tag="PLU43_02425"
     CDS             complement(1236..1913)
                     /locus_tag="PLU43_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO27292.1"
                     /translation="MKDHRGFSLVELLVAMAVGTIISAGIVSLIVISMRMYHNETVDA
                     NMQYEVQSALNQVTDAAMESQGLVISYDSAAETTDYVVLGTFKELADGSYSFTGSVFV
                     PDLTNETVYMMRVKSTALTGSSPQAIASSAVSTIQNASDKEKYLLSESVTKFYVGVAS
                     TGENKPLDATVSPPVYENPIALTIALAFEMDGTNKTIHKQVQDQVAIRNYVTSDIYVN
                     GTAYELK"
     gene            complement(1935..2456)
                     /locus_tag="PLU43_02430"
     CDS             complement(1935..2456)
                     /locus_tag="PLU43_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO27293.1"
                     /translation="MMKKEREKNKLNNNGYTLIELLVVIGIITVLVGGMSIAVSLIFS
                     KDAAKCAASINDQLSNARMLSMSREGVYTMKVNMDPAHEDNYVEISTASGGEYTKIDL
                     GDRVIISFGPDGGTLGTGTVEIVFDKASGAVRSVDGSETIADIYLFHIESVRGTRTSE
                     VELVSGTGRHYIR"
     gene            complement(2481..>3767)
                     /locus_tag="PLU43_02435"
     CDS             complement(2481..>3767)
                     /locus_tag="PLU43_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27294.1"
                     /translation="IDFTGKSGITPADTDKEMETAEAVAVKSNQEIYLIPNEFLAVSN
                     PVTKAVYSSMTDDPFAMTIPSEQDLAPTDWFGYKYIVKTDSVNGDYVEAEVKTMTRGS
                     VEYKYGYLSFNDKYFRQNGSGIYVECSQTDTGAMNAKAAYFDEIMNASADASSEAQPC
                     AYELKQRAMNTMFTNVSGTSMFDLNYLITGDGSTDFHLYAKNALVNYNSSGKTANVLC
                     NTEGIDRYAGYAQNFNHRYQNLYCYLDAKSDIPISQDPGAVEDSVLADTALPISSYID
                     VSAIKAAYSDPEDATLDAICTCVIKKVPTYTITGTFSGIAIIDGDIEVESGAKIDGLL
                     AATGTILFKGNNQVTSDRSLIQKRIDKEVELMKSAPEGTALPDTYLIAYLLDSAGNQM
                     YQITPVGEEEAARIDTDYTKFIRYDNWRKGDTVTVY"
BASE COUNT          901 a         1009 c          718 g         1139 t
ORIGIN      
        1 acgtatgaat cccattttct ccgtccgcac cgtcgattgc cagacagaac agcttaaaca
       61 tctcattatt gatcgttttc gatttcagat aatcgtacag ataaatattt ccgccgtctt
      121 caaaatccga ttccgttgta cattccagag acagactgtt gtcatcgtgc cagacatagt
      181 caaaattccc cgtattggaa aaattatcct tgcataactt tgaaaccatg catatattgg
      241 agattgcata ttgataatta taatctgtcg tatcagtgac cgccgccgtc ttctctatcc
      301 actgatccgg gtcgatataa gcacagtatg ccccctcctt cggattgtag ctgatcgcat
      361 ccacaacata atttttcaca ccgctcgtat tccagtcggt agttacgaac gtgacgtcat
      421 aatcactttt tccggtcgta tcatatacat agatctgctc agtgtcgtca aatgaataat
      481 acccgcttcc tttattctct gtcacggtat ccactttata ataaaccgta ttgctcgtct
      541 gtgtggtctg gtttttaaaa gaaatatcat aattattaac cgtatatttg agcaatacat
      601 gttcccatgt gtcttccgta ggtctgtcat cttttggcac ataatattta atagttgcct
      661 tgaactctcc gtctcctgcc gtaacaggaa ggtaatcata ttccgtactc tccgatgtcg
      721 atatcgcata taacccttct ccagaggtca ccttttcaaa atatcccgta taggttcccc
      781 agcttcttac gactgccctg acatctcctg cattgccaat atgcggggta gttaaatacg
      841 ttgtatagcg tccattggta cttcctgtta tgctgtttga aagagcatcc attccaaaag
      901 tcccgatcgg ttcctttccg ctgatcagat agccgatcat tgcataggtc gtatccggaa
      961 cgatctctaa aatcgtaaac gttgattttc cgccgtcagc accggcttct ttttgtttga
     1021 ctacttcggt cattaactgc aggggatccg ttgtctcctc tgtctgctgt aatgtatcgg
     1081 catcttccgt agtcccggtg tgcattctgt caaaaaaacc gcagacaaca gcacctgcca
     1141 tactcacggc aagtatcgct gctgccgcag atattacggc aatccgcttc agtattttat
     1201 ttttcttctg attttccctt ttcatccgca tatccttatt tcaattcata ggctgttccg
     1261 tttacataaa tatcagacgt cacgtaattg cgtattgcca cctgatcctg cacctgctta
     1321 tgaatggtct tatttgttcc gtccatctca aatgcaagcg cgatcgtaag agcgatcgga
     1381 ttctcatata ccggcgggct gactgtcgca tccagcggtt tattttctcc tgtcgacgca
     1441 acgccgacgt aaaattttgt tacggattct gaaagcagat atttttcctt gtctgaagcg
     1501 ttttggatcg tactgaccgc gcttgacgcg atcgcctgcg gagacgatcc cgtaagtgcc
     1561 gttgatttca cacgcatcat atagacggtt tcattcgtaa gatccggcac aaatacgctg
     1621 ccggtaaatg aatagctgcc gtctgccagt tctttaaaag ttccaagcac aacataatcc
     1681 gtcgtttccg cagcagaatc atagctgatg acaagcccct gtgactccat ggccgcatct
     1741 gtaacctgat tcagtgcaga ctgtacttcg tactgcatat ttgcatccac ggtttcatta
     1801 tgatacatcc gcatggaaat aacgatcagc gaaacgatcc ctgccgatat aatcgtaccg
     1861 actgccattg caaccagtaa ttcaactaat gaaaagcctc tgtgatcctt catatccgat
     1921 ccccctgctg cctgtcagcg tatataatgt cttcccgttc cgctgaccag ttctacctca
     1981 gaggtccttg tacccctgac actttctata tggaacaaat aaatatctgc aatcgtctca
     2041 ctgccgtcaa ctgacctgac tgctccgctt gccttatcaa atacgatttc cactgttcct
     2101 gttccaagcg ttccgccatc cggtccaaaa ctgatgataa ctctgtcccc cagatcgatc
     2161 ttcgtatatt ctcctcctga tgcagttgat atttcaacat aattatcttc atgagccggg
     2221 tccatgttga ccttcatcgt atagacgccc tcgcgtgaca tcgaaagcat ccgcgcatta
     2281 ctgagctggt cattaatgga ggccgcacac ttggcggcat ccttgctgaa gatcaaactg
     2341 acggcaatcg acatgccgcc gacaagcacc gtaatgatcc ctataactac aagcagctct
     2401 atcagcgtat atccgttgtt attcaacttg ttcttttccc gttctttttt catcatacca
     2461 cctgtatccg tctttcacat ctaatatacg gtaaccgtgt ctcccttacg ccagttatca
     2521 taacggataa atttcgtata atccgtgtct atccttgccg cctcttcttc cccgacaggc
     2581 gtgatctgat acatctggtt tccggcactg tcaagcagat aagcgatcag atatgtatcc
     2641 ggcagtgctg tcccttccgg tgcgcttttc ataagctcga cttccttatc gatccgtttc
     2701 tgaatcagtg aacgatcaga cgtcacctga ttgtttccct taaacaggat cgttcccgtc
     2761 gccgccagca gtccgtcgat ctttgcgccg gactctacct caatatcccc gtcgattatc
     2821 gcaattccgc tgaatgtgcc ggtaatcgta tacgtcggca cctttttgat cacacatgta
     2881 cagatcgcat ccagcgtcgc atcttccgga tcggaatagg cagcttttat ggcggacaca
     2941 tcaatataac tgctgatcgg aagagccgta tcggcaagca cgctatcctc tactgccccc
     3001 ggatcctgtg agatcggaat atcgcttttc gcatccagat aacaatacag attctgatat
     3061 ctgtgattga aattctgcgc atatccggca taacgatcga tgccctccgt attgcacagc
     3121 acatttgcgg tctttccgga actgttataa ttgacaagtg cattctttgc atacaaatga
     3181 aaatccgtac tgccgtctcc ggtaatcaga taattcagat caaacatcga agtgccggat
     3241 acatttgtga acattgtatt catagcccgc tgtttcagct cataagcaca cggctgtgcc
     3301 tctgatgatg cgtctgccga cgcattcatg atctcatcaa aatacgctgc tttcgcgttc
     3361 atggctcccg tatcggtctg cgagcattcc acataaatac cgcttccatt ttgtctgaaa
     3421 tatttatcat taaaggacag atacccgtat ttatattcca cgctgcctct tgtcattgtt
     3481 tttacttccg cttccacata atccccgtta acggaatcgg tctttacaat atatttatat
     3541 ccgaaccagt cggtcggtgc cagatcctgc tcggacggaa tcgtcatggc aaacggatca
     3601 tccgtcatgg aactgtaaac agcctttgtc accggattgg acactgcgag gaactcattc
     3661 ggaatcagat agatctcctg attcgatttt acggcaacag cttctgccgt ctccatttcc
     3721 ttgtcggtgt cggccggtgt gattccggac tttcccgtaa agtcgat
//
LOCUS       DAOSYE010000092         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17107_length_3765_cov_2.694070, whole genome shotgun sequence.
ACCESSION   DAOSYE010000092 DAOSYE010000000
VERSION     DAOSYE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17107_length_3765_cov_2.694070"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLU43_02440"
     CDS             complement(<1..514)
                     /locus_tag="PLU43_02440"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27295.1"
                     /translation="MQTELINLKGIGPKTAASYHKLGIYSCKDLLYYYPRDYIKFEEP
                     KPVCEEQLFKLCFFKAEIARQPLMRRAGKLQIVTAELLSGQTVISAVWFHMPYLTKSL
                     TVGKTFVFKSVLQKKGNRYHVEQPLFYTEEQYEKLLHTLQPVYPLIKGISSQAVGKLV
                     SQVLLQADISD"
     gene            complement(539..2239)
                     /locus_tag="PLU43_02445"
     CDS             complement(539..2239)
                     /locus_tag="PLU43_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOO27296.1"
                     /translation="MNKIDAKTFSKMFLAGAKTLESKKEWINELNVFPVPDGDTGTNM
                     TLTIMSAAKEVIALEDPTMQTLSKAISSGSLRGARGNSGVILSQLLRGFTKIIREYDE
                     IDTEILAAAMQKATETAYKAVMKPKEGTILTVARGASDKAMELVAKTDDLSVFMDEII
                     KHADYVLSQTPEMLPVLKQAGVVDSGGQGLVEVLKGGYDAYLGKEIDLTIISPEKTAS
                     EKIAAQAEMDIKFGYCTEFIIMVNNVFNAINEQDLKVYLESIGDSIVCVADGDIVKVH
                     VHTNDPGLAIQKALTYGDLTNMKIDNMREEHREKLFQMAECNAADEAEETSVSDENTE
                     DIQPLPEETDVSEKEIGFIAVSVGEGINAIFRGLGVDYLIQGGQTMNPSTEDMINAIE
                     KVPAETIFILPNNKNIILAANQAQYLVENKTIIVIPTKTVPQGITALINFMADKSPEE
                     NAEIMKEEIKKVKTGQVTYAVRDTVINGLEIKQGNIMGIGDHDILSVGTVVEDTAMEM
                     LTKMVDDDSELISIYFGNEVDEEMAGDFAAEVEKNYPECDVEIHTGGQPIYYYIISVE
                     "
     gene            complement(2259..2615)
                     /locus_tag="PLU43_02450"
     CDS             complement(2259..2615)
                     /locus_tag="PLU43_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOO27297.1"
                     /translation="MKGHVNTQVGNISIDTEVIAQYAGSVAVECFGIVGMASVNMKDG
                     IVKLLRRESITHGINVEIDNNQLILDFHVIVAYGVSIQAVSDNLISNVKYKVEEFTGL
                     TIEKINIYVEGVRVID"
     gene            2882..3067
                     /gene="rpmB"
                     /locus_tag="PLU43_02455"
     CDS             2882..3067
                     /gene="rpmB"
                     /locus_tag="PLU43_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOO27298.1"
                     /translation="MAKCDICGKGVHFGNNVSHSHRRSNRIFKSNVKRVKCKVNGIPK
                     RLHVCTQCLKSNKVERA"
     gene            complement(3115..3498)
                     /locus_tag="PLU43_02460"
     CDS             complement(3115..3498)
                     /locus_tag="PLU43_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOO27299.1"
                     /translation="MAENNFNEVVESLIKGMNGFLSTKTVVGEATKIDDTIIVPFVDV
                     SFGVGAGEFKSDKKENGAGGLSGKMAPSAVLVIQKGTTKLVNIKNQDSMTKILDMIPD
                     FVNKFASKKEEKVSDDDAVNAAFDE"
     gene            complement(3499..>3765)
                     /locus_tag="PLU43_02465"
     CDS             complement(3499..>3765)
                     /locus_tag="PLU43_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOO27300.1"
                     /translation="KPQKFRLALHIGMDDPAQTGQALAILGVVYPFFEKGLTVVPEFE
                     KSVFEFDLSVKGRITVFVFLRAAWKIYFNRDFKRMLHILKKEAA"
BASE COUNT         1042 a          867 c          649 g         1207 t
ORIGIN      
        1 catcggaaat atcggcctga agaagcacct ggctaactaa tttaccgact gcctgactgg
       61 atatgccctt tatcagcgga taaaccggct gcagtgtatg aagcagtttt tcatactgtt
      121 cctcagtata aaataaaggc tgttccacat gataccgatt tcctttcttc tgtaaaacgc
      181 ttttaaaaac aaacgttttt ccgactgtca gactttttgt cagatatggc atatggaacc
      241 ataccgccga tatgacagtc tgaccggaaa gaagctctgc tgttacgatc tgaagtttac
      301 cggccctgcg cataagcggc tgtctggcaa tttctgcttt aaaaaagcaa agcttaaaaa
      361 gctgttcctc acatactggt tttggctctt caaattttat ataatcacgc ggatagtaat
      421 acagcagatc cttacaggaa taaattccaa gtttatggta agaagctgct gtttttggtc
      481 cgattccttt taaattgata agttcagtct gcatatccat ggcttctccg aaaataaact
      541 actccacaga aataatatag tagtagatcg gctgcccgcc cgtatggatc tccacatcac
      601 attccggata atttttttca acttcagcgg caaaatcacc ggccatctcc tcatcgactt
      661 catttccgaa ataaatgctg atcagttctg agtcgtcatc caccattttt gtgagcattt
      721 ccattgcagt atcttcaaca accgttccga cggaaagaat gtcatgatca ccgatcccca
      781 taatattgcc ctgcttgatc tccagtccgt tgatcacggt atcgcgtacg gcataagtca
      841 cctggccggt ctttactttc ttgatttctt ccttcatgat ctccgcattt tcttcgggag
      901 atttatctgc catgaaattg atcaatgcag taataccctg aggaacagtt ttcgtcggaa
      961 taacaataat cgttttgttt tcaactaaat actgtgcctg attggcagcc aaaataatat
     1021 ttttattgtt aggcaaaata aatatagttt ctgccggaac cttttcgatc gcattgatca
     1081 tatcttccgt actgggattc atcgtctgtc cgccctgaat cagataatcg acaccgagtc
     1141 ccctgaagat cgcattgatg ccttccccta cggaaactgc tataaaaccg atttcttttt
     1201 cggaaacatc agtttcttcg ggaagcggct gtatatcttc cgtattctca tcagaaacgg
     1261 atgtttcttc cgcttcgtct gccgcattgc actctgccat ctggaacagc ttttcccgat
     1321 gttcttcacg catattatct attttcatat ttgtaagatc gccataagtc agcgctttct
     1381 ggatcgcaag accaggatca tttgtatgca catgaacttt tacgatatct ccgtctgcca
     1441 cacagacaat ggaatccccg atcgattcca gataaacttt cagatcctgt tcatttattg
     1501 cattgaacac attatttacc ataataatga attccgtaca gtagccgaat ttaatatcca
     1561 tctctgcctg agccgcaatc ttttctgatg cggttttttc cggagatatg atagtcagat
     1621 cgatctcctt gccaagataa gcgtcatagc cgccctttaa tacttcgaca agtccctgac
     1681 cgccggaatc aacaacgcct gcctgcttta atacaggaag catttccgga gtctgactta
     1741 aaacatagtc ggcatgctta atgatctcat ccataaaaac agacaggtca tcagtctttg
     1801 caacaagttc catcgctttg tcggaagccc ctctggcaac tgtcaggatc gtaccttcct
     1861 ttggtttcat aactgcctta tatgcggttt ccgtagcctt ctgcatggca gcggcaagaa
     1921 tttccgtatc gatttcatca tactctctga tgatttttgt aaaacctctt aaaagctgtg
     1981 ataaaataac gcccgagttt cccctggcac ctcttaaaga tcctgatgaa atcgctttgg
     2041 agagcgtctg cattgtcgga tcctcaagtg caattacttc tttagccgca gacatgatcg
     2101 tcaaagtcat attcgtgcct gtgtctccgt ccggcaccgg aaatacgttc agctcattga
     2161 tccattcttt ctttgattca agcgtctttg ctccggccaa aaacatcttg gaaaacgttt
     2221 tggcatcgat cttgttcatc cccacttcta tttcctcctt aatcgattac tctaacacct
     2281 tctacataga tgtttatttt ttctattgta agtcccgtaa attcttcgac tttatatttc
     2341 acattactga tcagattgtc cgatactgcc tgaatactga caccatatgc cacaattaca
     2401 tggaaatcca gaatcagctg attattatct atttctacat taatgccgtg cgtgatgctt
     2461 tctcttcgta aaagcttcac gatcccatct ttcatattaa cagaagccat tccaacgata
     2521 ccaaaacact caactgctac tgagccggca tattgtgcaa ttacttctgt gtctatgctg
     2581 atatttccga cctgtgtatt cacatgaccc ttcatagata ttcctccgtt ctcttctcac
     2641 cgtacgcact attattaaga ttacaatatt gttttaaaaa agtaaacaat aaaaacaaat
     2701 gaattaacca gaaatttata aagcataata attatacacg ttttctaagt gaattggaac
     2761 atacaaaata tatttttctg ttttggcatc ttttgcttgc aattatatag aacttttgat
     2821 agaatatgtt tgttgcgata aaaacaagca attcttatgg acttttaagg aggtgcgacc
     2881 gatggctaaa tgtgatattt gcggaaaagg cgttcatttt ggtaataatg taagtcattc
     2941 tcatagaaga tcgaacagaa ttttcaagtc taatgtgaaa cgtgtgaaat gtaaggttaa
     3001 tggtattccg aagagacttc atgtttgtac acagtgtttg aaatcaaaca aagttgaacg
     3061 tgcataataa aaaagcaatc cgaatgggtt gcttttttat ttatgaaaac cgaattattc
     3121 atcaaatgcc gcatttactg catcatcatc cgaaaccttc tcttcttttt ttgaagcaaa
     3181 tttattaaca aaatcaggaa tcatatcaag aatttttgtc atgctgtcct ggtttttgat
     3241 attaacaagc tttgtagtac ctttttgaat gacaagtacc gcgcttggcg ccattttacc
     3301 gctgagtccc cctgccccgt tctctttttt atcgctttta aattctccgg ctccgacacc
     3361 aaacgataca tctacaaagg gtactatgat tgtatcatcg attttggtag cttcgcccac
     3421 aaccgttttt gtggaaagaa atccattcat acctttaatc aatgattcca cgacttcgtt
     3481 gaaattgttt tcagccattt atgcagcctc cttttttagt atatgtaaca tacgcttaaa
     3541 atccctgttg aaatatattt tccacgcagc ccgcaaaaac acaaatacgg taatgcggcc
     3601 ttttacagat agatcaaact caaatacact tttttcaaac tccggaacga ccgtgagacc
     3661 tttttcgaaa aaaggataaa ccactccgag aattgccagc gcctgccctg tctgtgcggg
     3721 atcgtccatc ccaatatgaa gagccaggcg gaatttttga ggttt
//
LOCUS       DAOSYE010000093         3761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17127_length_3761_cov_3.104965, whole genome shotgun sequence.
ACCESSION   DAOSYE010000093 DAOSYE010000000
VERSION     DAOSYE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17127_length_3761_cov_3.104965"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLU43_02470"
     CDS             <1..424
                     /locus_tag="PLU43_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27301.1"
                     /translation="FMTENFDCAVLPMSNDGGKASIFNGLGNAIAATCEHPDQAWKWV
                     EYLSSKEGQERQAGLGIAISAYNGTADKFTEAYPMFNTKCYIDMVDYAQIRPYTNQTS
                     VWEDKAYELLKDAYAGKEEVSDACAATAEMMNEAIAAE"
     gene            513..1415
                     /locus_tag="PLU43_02475"
     CDS             513..1415
                     /locus_tag="PLU43_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27302.1"
                     /translation="MQKHKIPKKRWNEMCWAWFMVAPTIIGLLILNIIPIFQTFYLSF
                     FKSGAFGKGNIFVGFDNYKTLIHDVQVWYAVRNTLSYTCAVVPITIILALIAAVLLNG
                     KVKGRNIYRTIYFIPMVAAPAAVTMVWKWLYNYQFGLINHFLEAVGLEKVNWIDDPDV
                     AMISIVIIGVWSAFGYCMVLLLAGLQEIPRDYYEASNIDGASPVKQFFSITLPLVSPT
                     LFFVLVTSIIQAMQVFDVIYMMLDVTSPSYDTTVSLVYLFYNNSFKYSNKGYGSAIVM
                     LLLLIIMIITVIQTKLQKKWVNYM"
     gene            1429..2259
                     /locus_tag="PLU43_02480"
     CDS             1429..2259
                     /locus_tag="PLU43_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27303.1"
                     /translation="MRKRKLINSMIIQVVLLLGAAVMILPFVWMVITSLKTITEATSM
                     NPFVFLPKKAQFENYLNVIKQNDFLRLYLNTFAMMVFRVFCAVIFSAMAAYAFARLEF
                     PGKNFLFGLVLFQMMVPVQIFVIPQYLMIDKLGARNTIFALVFPGLVSAFGTFLLRQF
                     FMGLPKELEESSKLDGCNIGQTFYLVMLPLTRSGLVALAIFTALFSFKDLMWPLIVNT
                     DSDAAVLSSALAKISSAYSVNYPELMAASVLAIWPMLLVYIIFQKQFIEGIATSGGKL
                     "
     gene            2287..>3761
                     /locus_tag="PLU43_02485"
     CDS             2287..>3761
                     /locus_tag="PLU43_02485"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO27304.1"
                     /translation="MPIIYEEESREFHIYNNEISYIIQVLDNQTLGNLYYGKRIRHKP
                     DFQYLLQGGRRSLAVYNQKNEYFLSPQYTKLEFPVYGTGDFRYPMIQIKQENGSRITN
                     FEYCSHVIKKGKTGLTGLPATYAESDKEATTLEITMTDPLIHVDCVLSYTVFEWGPAI
                     ARSVSVKNEGTKDVTVTRLLSASVDLPDSDFELLSLSGAWSRERQLKRRKIEQGVQSV
                     YSMCGTSSAEHNPFVVLAREETDERAGEAYGFSLVYSGNHLEQVEVDTSDLTRVSLGI
                     HPDTFEWPLAQGEVFQAPEAVLVYSDHGLNYLSQVYHKLYRTRLARGIWRDKERPILI
                     NNWEATEMNFSEAHILKMAATAKKMGIEMFVLDDGWFGNRDDDTSSLGDWYVKNYEKL
                     PNGIEGLADKIEQLGMKFGLWIEPEMVNMDSDLYRLHPDWILCAPGRTPSPSRNQYVL
                     DFSRAEVVDYIYGLLERLFAKARISYVKWDMNRYLTECYSRA"
BASE COUNT         1081 a          668 c          955 g         1057 t
ORIGIN      
        1 ttttatgacc gagaattttg actgtgcagt gctgccaatg tccaatgacg gaggaaaggc
       61 ttctatcttt aacggacttg gcaatgcgat cgcagcgacc tgcgagcatc cggatcaggc
      121 atggaaatgg gtggaatatt tgagcagcaa ggaaggccag gaacggcagg cagggcttgg
      181 aatcgctatt tccgcttaca atggtacggc agataaattt acagaagctt atccgatgtt
      241 caatacaaaa tgttatatcg acatggtcga ttatgcgcag atcagaccat acacaaatca
      301 gacaagcgta tgggaagaca aggcgtatga actgctgaag gatgcctatg caggaaagga
      361 agaagtttct gacgcctgcg cagcaacagc agaaatgatg aatgaagcaa tagcggctga
      421 ataaacgtta ttcgaaaaat ttagaaaggc atgacaggac ttgccgctgt ggtaagtcct
      481 gtgtgttggt aaggagagaa tttatgtcct ggatgcagaa gcataaaata cccaagaaga
      541 gatggaacga aatgtgctgg gcatggttta tggttgcgcc tacgatcatc ggactgctga
      601 tactaaatat tattcctatt tttcagacat tttatttgag cttttttaag agcggtgcat
      661 ttggaaaagg taatattttc gtcgggtttg ataattataa aacactgatc catgatgttc
      721 aggtatggta tgcggtaagg aatacgcttt catatacgtg cgcagttgtt ccgatcacga
      781 tcattctagc cttaatagcc gcagttctgc tgaatggaaa agtgaaaggg agaaatatct
      841 accgtacaat ttattttatt ccaatggtag cggcgcccgc tgcggtgaca atggtttgga
      901 aatggcttta taattatcag tttggcttaa tcaatcattt tctggaagcg gtaggactgg
      961 aaaaagtaaa ctggatcgat gatccggatg ttgcaatgat atctattgtg atcatcgggg
     1021 tctggagcgc gtttggatat tgtatggttc tgctgctggc agggcttcag gaaattccga
     1081 gagattatta tgaggcgtcc aatatagacg gggcgtcccc tgtaaagcag tttttttcca
     1141 ttacactgcc gcttgtttcg ccgacgctgt tttttgtttt agtaacaagt attattcagg
     1201 caatgcaggt atttgacgtg atctatatga tgcttgacgt aacgagccct tcttatgata
     1261 cgacggtatc gcttgtatat ctgttctata ataattcttt caaatattca aataaagggt
     1321 atggttcggc aatcgtcatg cttctgcttc tgattattat gatcatcacc gtgatccaga
     1381 caaagctgca gaaaaaatgg gtcaattata tgtaaaagga gaattaaaat gcggaaaaga
     1441 aaactgataa attcaatgat tatacaggtg gtattattgc ttggcgcggc agttatgatc
     1501 cttccttttg tctggatggt catcacttct ttaaagacga tcacagaagc gacttccatg
     1561 aatccatttg tattcctgcc aaaaaaagca cagtttgaaa attatctgaa tgtgatcaaa
     1621 cagaatgact ttttacgcct gtatttgaat acgtttgcca tgatggtgtt tcgtgtattc
     1681 tgtgctgtta ttttcagcgc catggctgcc tatgcgtttg caaggctgga atttccgggc
     1741 aaaaattttt tatttgggct tgtattgttc cagatgatgg tgccggttca gatctttgtg
     1801 attccacaat atctgatgat tgataagctt ggggccagaa atactatttt tgcgcttgta
     1861 tttccgggac tcgtcagtgc attcggaaca tttctgctgc ggcagttttt tatgggactg
     1921 ccaaaggaac tggaagaatc atcaaagctt gacggctgta atatcggtca gaccttttat
     1981 ctggtcatgc tgccgcttac cagatcgggg ctggtagctc tggcgatttt cacagcatta
     2041 ttttcgttta aggatctgat gtggccgctg atcgtaaata cagattcgga tgcggccgtg
     2101 ctttcttcgg cattggccaa aatttccagt gcatattctg ttaattatcc ggagttgatg
     2161 gcagcgtcgg tgctggcgat ctggcccatg ctcctggtgt atataatatt ccagaagcag
     2221 tttattgagg gaattgcaac ctctggagga aaattgtaat aatggataaa tgctaaggag
     2281 attattatgc ccatcatata cgaggaagaa tcaagagaat ttcatatata taacaatgaa
     2341 atcagctata ttatacaagt actggacaac cagacattag ggaatctgta ttacggaaag
     2401 cggatacgtc ataagcctga ttttcaatat cttctgcagg gagggagacg ttcgctggca
     2461 gtctacaatc agaaaaatga atatttttta agtccgcagt atacgaaatt ggaatttccg
     2521 gtatatggta cgggagattt cagatatccg atgatacaga tcaaacagga gaatggaagc
     2581 cgtattacaa attttgaata ctgttcacat gtaatcaaaa agggaaaaac aggactgaca
     2641 gggcttccgg ctacttatgc ggagagtgat aaagaagcca ctacattgga gatcacaatg
     2701 acagacccgc tgatccatgt ggactgtgta ctcagttata ctgtttttga atggggaccg
     2761 gcaattgcaa gaagtgtgtc cgtaaaaaac gaaggaacaa aagatgtgac ggttacaaga
     2821 ctcctgagtg ccagtgttga tcttcctgat tctgattttg aattattgag cctgtcggga
     2881 gcgtggtcca gggaacggca gttaaaaaga agaaagatcg agcagggcgt acagagcgtg
     2941 tacagtatgt gcgggacaag cagcgccgaa cacaatccgt ttgtagtgct tgccagggaa
     3001 gaaacggatg aacgggcagg agaagcttat ggattcagcc ttgtatacag tggaaatcat
     3061 ctggaacaag tcgaggtaga tacgagcgat ctgacaaggg tttcgcttgg aattcatccg
     3121 gatacatttg agtggccgct tgcgcaggga gaagtatttc aggcgccgga agcagtgctg
     3181 gtctattcgg atcacggatt gaactattta agccaggtct atcataagct ttaccgcaca
     3241 agactggcca ggggtatctg gcgggataag gaacggccga tcctgatcaa taactgggaa
     3301 gcaacggaga tgaatttttc ggaagcccat atattgaaaa tggctgcaac tgcaaaaaaa
     3361 atgggaattg aaatgtttgt tcttgatgat ggctggttcg gaaacagaga tgacgataca
     3421 agttctcttg gagactggta cgtaaaaaat tatgaaaaac ttccgaatgg aatcgaagga
     3481 ctggctgaca aaatagaaca gcttggaatg aaattcgggc tgtggataga accggaaatg
     3541 gtgaatatgg attctgatct atacagactg catccggact ggatcttatg tgcgccggga
     3601 aggacaccgt ctccgtccag aaaccagtat gtgctggatt tttcaagagc agaggtggtg
     3661 gattatattt acggtctgct ggaacgtctg tttgcaaagg caaggatctc ctacgtgaag
     3721 tgggatatga accgttatct tacggaatgt tattccaggg c
//
LOCUS       DAOSYE010000094         3739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17289_length_3739_cov_3.192182, whole genome shotgun sequence.
ACCESSION   DAOSYE010000094 DAOSYE010000000
VERSION     DAOSYE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17289_length_3739_cov_3.192182"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1082)
                     /gene="fmt"
                     /locus_tag="PLU43_02490"
     CDS             complement(138..1082)
                     /gene="fmt"
                     /locus_tag="PLU43_02490"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOO27305.1"
                     /translation="MKIVFMGTPDFAVKILEAILRSPHEVTAVVTQADKPKGRGMELS
                     QTPVKKFAVEAGIPVFQPEKIKAPESVDMLKTFEADIFVVAAFGQILSGEILRMPSHG
                     CINVHASLLPKYRGAAPIQWAIINGEKETGVTIMQMDEGLDTGDMLFKTVVLIDAEET
                     GGSLHDKLAEAGAGLCVEALDRIAEGRIEPVGQEEGVSSYAKMLTKALGFLQFEKKAA
                     ELERMVRGLDPWPGTYTYYYGKTLKIWKAKAADETLNGRPGTIARVETDAFYINTGEG
                     LLKIMELQLEGKKRMTAAEFLRGRRLSAGELLCEQPHK"
     gene            complement(1079..1573)
                     /gene="def"
                     /locus_tag="PLU43_02495"
     CDS             complement(1079..1573)
                     /gene="def"
                     /locus_tag="PLU43_02495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOO27306.1"
                     /translation="MALRTIRVIGDEVLTKKCKEVKEMTPRTKILIEDMLETMYAEEG
                     VGLAAPQVGILKRIVVIDTGDGPHVLINPKLVETSGEQTGNEGCLSVPGKVGIVTRPN
                     YAKAVALDVNMEPIEIEGTELKARAICHELDHLEGQLYVDKVEGKLMSLEELNEEQEE
                     EEQA"
     gene            complement(1626..>3739)
                     /gene="priA"
                     /locus_tag="PLU43_02500"
     CDS             complement(1626..>3739)
                     /gene="priA"
                     /locus_tag="PLU43_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOO27307.1"
                     /translation="INGYVLEITDRAEFAPEKQKEIAAVVHDSSPADKNMIRLAFWMK
                     ENYGSTMITALKTVLPIKKKIRKKEEKTVRLLLTKEAAIEQLAFFEKKHQTARARILR
                     ELLQETCIDYRLVTGKLNIVPATLQLMAEQKILCVEAKRIYRNTVHGETETEKKILNE
                     FQRPVADEIISDYRENIRKTYLIRGVTGSGKTEIYMEVIEEVVRSGRQVIVLIPEIAL
                     TYQTVMRFYRRFGERVSTLHSKLSDGERYDQFERAKCGEIDVMIGPRSALFTPFERLG
                     LIVIDEEHESSYKSDAMPKYHAREVAQELAGMYGASVILGSATPSMEAYEKALRGEYK
                     LFSLDNRVLGASMPRVSIVDLREELKEGNRSVFSRMLREKIEDRLTKGEQVMLFLNRR
                     GYAGFVSCRSCGHVMKCPHCDVSLSKHGNGRLVCHYCGYEQDDVKVCPECGSSFIGGM
                     RAGTEQIEMQIKKAFPYAAVLRMDADTTKKKGGYDNILSAFANRDADILVGTQMIVKG
                     HDFPYVTLVGILAADLSLHVNDYRASERTFQLLTQAAGRAGRAERPGEVVIQTYNPDH
                     YSILAAAKQDYPAFYKEEIGYRSLMDYPPAGHMLAMLLEGTGEEDTERFSSELADRLK
                     DDILNGRISEKQPGKAVFLIGPADASVRKINDIYRRIIYLKSKDYESLKKLKDSAETF
                     FDREKPQTVRMTFDFDPVSGY"
BASE COUNT          865 a         1081 c          679 g         1114 t
ORIGIN      
        1 cttcgcatac ttggaatacg tggatttcac tcttgccgaa gcccagatcg taagtatcat
       61 gccgattatg acaagaagaa atgtactgtc aaaataataa taattgtaat agcccatatg
      121 gctcatctcc ttttcaacta tttatgcggc tgctcgcaaa gcagctctcc tgccgacagc
      181 ctgcggcccc tcaaaaattc cgctgccgtc atccgtttct ttccttccag ctgcaattcc
      241 atgattttta acagcccttc gcccgtattg atataaaatg catcggtctc cacccttgca
      301 atcgtaccgg gccgcccgtt caatgtctca tctgccgctt ttgctttcca tattttcaat
      361 gtctttccat aataatatgt ataagtaccc ggccatggat ccagtcccct gaccatacgc
      421 tccagttctg cggctttttt ctcaaattgt aaaaatccaa gtgcctttgt cagcattttg
      481 gcataagaag aaactccttc ctcctgtccg acaggttcga tccttccttc tgctatcctg
      541 tcaagcgcct caacacaaag gcccgctcct gcctctgcca gcttgtcgtg aaggcttcct
      601 cccgtttctt ccgcatcgat caaaacaacg gtcttaaaca gcatatctcc cgtatccagt
      661 ccttcatcca tctgcatgat cgtcacaccg gtttcttttt ctccgttgat gatcgcccat
      721 tgtatcggtg ccgctcctct gtatttcggc agcagcgatg catgcacatt gatgcagcca
      781 tgagacggca tccgcagtat ttctcctgat aagatctgcc caaacgcagc aacgacaaaa
      841 atatccgctt caaaagtctt taacatgtcc acagactcag gcgcctttat tttctccggc
      901 tgaaaaaccg gtattccggc ttcgaccgca aatttcttga caggagtctg cgacagttcc
      961 attcccctgc ccttcggctt atccgcctgc gtcacaacag cagtcacctc atgcggactc
     1021 ctaaggatcg cttccaggat cttcaccgca aaatccggcg ttcccataaa cacaattttc
     1081 atgcctgttc ctcttcctcc tgctcttcat tcagctcttc cagactcatc agtttgcctt
     1141 cgaccttatc aacatacagc tgtccctcca gatgatccag ttcatgacag atcgcacgcg
     1201 cttttaattc agtcccttcg atctcgatcg gttccatatt gacatcaagt gcaactgctt
     1261 ttgcataatt aggccttgtc acgataccga cctttcccgg aacgctcaga catccttcat
     1321 ttcctgtctg ttctcccgac gtttccacaa gctttggatt gatcagcaca tgcggaccgt
     1381 ctcctgtgtc gatcacaacg attctcttca gtataccgac ctgcggtgcc gcaagtccta
     1441 ctccttcttc cgcatacatc gtttccagca tgtcttcgat caatattttt gtacgcggcg
     1501 tcatttcctt gacttccttg catttctttg tcaaaacctc gtcacctatt accctgattg
     1561 ttcttaatgc catccttttt ccctctttcc tgcattataa aattctgcct gctgctaata
     1621 tctgcttaat aaccgctcac cggatcaaaa tcaaatgtca tgcggacggt ctgcggtttc
     1681 tctctgtcaa aaaacgtttc cgcactgtct ttcagtttct tcagtgattc ataatccttt
     1741 gatttcaaat aaatgatcct gcggtaaata tcattgatct tacgcaccga tgcatcggcc
     1801 ggaccgatca gaaatacagc ctttcccggc tgcttttccg atatcctgcc attaagtata
     1861 tcatctttta agcggtctgc caattcactg ctgaatcgtt ccgtatcttc ctctccggtc
     1921 ccctctaaaa gcatggcaag catatgtccg gcaggcggat aatccatcaa agaacggtat
     1981 ccgatctctt ctttataaaa tgcaggataa tcctgctttg cggcggcaag aatgctgtaa
     2041 tgatccggat tataggtctg aatgacaact tcacccggcc gttcggctct gcccgcccgc
     2101 ccggccgcct gcgtcagcag ctgaaacgtt cgttccgaag cccggtaatc attgacatga
     2161 agagacagat cggcagcaag tattcctacc agtgtcacat atggaaaatc atgtcctttt
     2221 acgatcatct gggtccctac aagaatatcc gcatcccggt ttgcaaaagc gcttaatata
     2281 ttgtcatagc cgcccttttt ctttgttgta tccgcatcca tccgcagaac ggccgcatac
     2341 ggaaatgctt ttttgatctg catttcgatc tgctccgtac cggcacgcat accgccgata
     2401 aacgacgaac cgcattccgg gcagaccttc acgtcgtcct gttcatatcc gcagtaatga
     2461 cagacaagcc tgccgtttcc gtgttttgac aatgacacat cgcaatgcgg acatttcata
     2521 acatgcccgc aggaacggca cgatacgaat ccggcatacc cccgtctgtt taaaaaaagc
     2581 atgacctgtt cgccctttgt aagccgatcc tctatttttt cccgaagcat tcggctgaat
     2641 accgaacggt ttccttcctt taattcctct cttagatcca caatggagac acgcggcata
     2701 gatgcgccaa gaaccctgtt gtcaagcgaa aatagtttat attctccccg cagcgctttc
     2761 tcatatgcct ccatagacgg tgtggcagaa cccaaaatga cagaagcacc gtacatcccg
     2821 gcaagctcct gtgcaacttc ccttgcatga tatttcggca tcgcatcact tttataagag
     2881 gattcgtgct cctcgtcgat cacgatcaga ccaagtcttt caaacggggt aaacaatgct
     2941 gacctgggcc ctatcatcac atcgatctcc ccgcatttag ccctttcaaa ctgatcatat
     3001 ctttctccgt ccgaaagttt ggaatgaagc gtagataccc gttctccgaa acgacggtaa
     3061 aaacgcatga cggtctgata agtcagtgcg atctccggaa tcaatacgat cacctgtctg
     3121 ccggatctta cgacctcctc gatgacttcc atatagatct ccgttttgcc gcttcccgta
     3181 actccgcgta tcaaataagt ctttcttata ttttccctgt aatcggaaat aatttcatcc
     3241 gccacaggtc tctgaaattc attcagtatt ttcttttccg tctctgtttc cccgtgtacc
     3301 gtattcctgt atatcctttt tgcctcgaca cagaggatct tctgctcagc catcaattgc
     3361 agcgtagcag gcactatatt cagctttccc gttacaagcc tgtaatcaat acatgtttcc
     3421 tgaagcaatt cccgcaaaat ccttgctctt gctgtttgat gttttttctc aaaaaacgca
     3481 agctgctcta tggcagcttc tttagtcagc agcagacgaa ccgtcttttc ttcttttttc
     3541 ctgatttttt tctttattgg aagaacggtt tttaatgccg tgatcattgt ggaaccataa
     3601 ttttctttca tccagaaagc aagccggatc atgtttttat ccgccggact gctgtcatgt
     3661 acaacagccg caatttcttt ctgcttttcc ggtgcaaatt cggcacgatc ggtaatttcc
     3721 agcacatatc cgttgatca
//
LOCUS       DAOSYE010000095         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17324_length_3734_cov_3.556129, whole genome shotgun sequence.
ACCESSION   DAOSYE010000095 DAOSYE010000000
VERSION     DAOSYE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17324_length_3734_cov_3.556129"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLU43_02505"
     CDS             complement(<1..1307)
                     /locus_tag="PLU43_02505"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO27308.1"
                     /translation="MLFSSISFLFFMTVVFLLYWALPHPYRWPALLAANVFFYASWDI
                     RFLAALVFTTAVSYGCALFMEKASASGKKKALLVSGILVTMFFLLFFKYANFTIYSVQ
                     KVLKLFAVPIQDTTLNLIMPFGISFYTFQMVGYLADVYKGKIKAEHHFGKYALFVSFF
                     PNISSGPIERAGRILPQIDKEKQFDYDTAVYGMRLILLGLLKKLVFADSMSKYVDIVF
                     DDVTNHSGICFIIATLLFTFQIYCDFSGYTDIAIGAAKLLGFDLMTNFKSPYWSSSIK
                     EFWSRWHISLSTWFRDYVYIPLGGNRVSKAKRNRNLMVTFLASGLWHGASWTFVLWGG
                     IHGLCQVAEDTIKEFHVKKRGGREHEQSGQKRLFASHLKHAGSILLTFCIVSFAWMFF
                     RANSISDVLYILRHMFTGGSSFSDAMSQMSMSRMSVVKTGAAI"
     gene            complement(1297..1734)
                     /locus_tag="PLU43_02510"
     CDS             complement(1297..1734)
                     /locus_tag="PLU43_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO27309.1"
                     /translation="MKISTKGRYAVRVMLDLAVHNTGEYIKVKQIARRQEISKKYLEQ
                     IIAVLNKAGYVKSVRGAQGGYKLAKEPKEYTVGMILRLTEGSLAPVACLEGETNECER
                     CDTCDTLAIWKQLECAINDVVDHVTVADLVERQQERLGSQYVI"
     gene            complement(1826..2761)
                     /gene="cysK"
                     /locus_tag="PLU43_02515"
     CDS             complement(1826..2761)
                     /gene="cysK"
                     /locus_tag="PLU43_02515"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOO27310.1"
                     /translation="MAKIKTSTTELIGGTPLLEISNYEKKHGLKARVLAKLEYLNPAG
                     SVKDRIALAMIEDAEQKGILKAGATIIEPTSGNTGIGIASVAAAKGYHAILTMPETMS
                     VERRNILKAYGAQVVLTNGTKGMKGAIEKAEELKAQIPGSVILGQFDNPANPAAHERT
                     TGPEIWDDTDGTVDFFVAGVGTGGTLSGTGKYLKQKNPDIKIIAIEPADSPVLSAGTP
                     GPHKIQGIGAGFIPQVLNRDIYDEIITVKNEDAFAAGREIALTEGILVGISSGAALHA
                     AAEIAGRPENEGKTIVVLLPDTGDRYLSTPLFTEE"
     gene            complement(2931..3407)
                     /locus_tag="PLU43_02520"
     CDS             complement(2931..3407)
                     /locus_tag="PLU43_02520"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO27311.1"
                     /translation="MGYRLLAVLLFGVDSQIKSAVEKKVKAGSVKKIWRDRLLIRKHH
                     NKGVMLNAFEKYQKLTAAVSAGLTVLVILLGEETFAGKGRNTAGKLGFACLIGGALSN
                     TFDRIRRGYVVDYMSFNVKRKKIKNLVFNISDFFIIAGVLITALSGEEKTREIKPV"
     gene            complement(3444..>3734)
                     /locus_tag="PLU43_02525"
     CDS             complement(3444..>3734)
                     /locus_tag="PLU43_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27312.1"
                     /translation="LDEVQLEFEEDALEEIAKKALEKDTGARALRSIIEEFMLDIMYE
                     IPKDENIGRVTITKDYILGKGGPLVDLRGGDLSFSERERLNAMGTPSLTAAK"
BASE COUNT          993 a         1003 c          651 g         1087 t
ORIGIN      
        1 aaaatggctg ccccggtctt tactaccgac atccggctca ttgacatctg gctcattgca
       61 tcggaaaacg aagaacctcc tgtaaacata tggcgcaaaa tatataaaac atctgaaatc
      121 gaatttgccc taaaaaacat ccatgcaaag ctgacaatac aaaacgtcag caaaatactg
      181 cctgcatgct tcagatgcga tgcgaataaa cgtttctgtc cggactgctc atgctccctg
      241 ccgcctcttt ttttcacatg aaattctttt attgtatctt ccgctacctg gcagagcccg
      301 tgaataccgc cccacagaac aaaggtccag cttgctccat gccagagacc gcttgccaga
      361 aacgtcacca tcaggtttct gtttctcttt gctttagata cgcggttgcc gccaagaggg
      421 atatatacat aatcacggaa ccatgtggaa agggaaatat gccagcggct ccagaattcc
      481 ttgatcgaag acgaccagta aggagatttg aaatttgtca tcaggtcaaa acccagaagc
      541 tttgctgctc cgatcgcaat atcggtatat ccagagaaat cacaatagat ctggaaagta
      601 aacagcagcg ttgcaatgat aaagcagata ccggaatgat ttgtcacatc atcaaagacg
      661 atatccacat attttgacat ggaatccgca aacacaagct ttttcagcaa gccgagcagg
      721 atcagcctca tgccgtatac tgccgtatca tagtcaaatt gcttttcttt atcaatctgc
      781 ggtaagatcc ttccggcacg ctcgatcggg cctgaagaaa tattcgggaa aaaagatacg
      841 aacagtgcat atttcccaaa atgatgctct gctttgatct tccctttata aacatcagcc
      901 agataaccaa ccatttgaaa tgtgtaaaaa gaaatcccaa aaggcatgat aagattcagc
      961 gttgtatcct gtatgggaac ggcaaagagc tttaagacct tttgcacgct gtatattgta
     1021 aaatttgcat atttaaaaaa caaaagaaaa aacatcgtaa cgagaatgcc ggacacaagc
     1081 agtgccttct ttttcccgga agccgacgcc ttttccataa aaagtgcaca gccgtaagag
     1141 actgccgtcg taaataccag cgccgcgaga aacctgatat cccagctggc ataaaaaaac
     1201 acatttgccg caagcagcgc aggccatctg tatggatgcg gcagagccca atagagcagg
     1261 aataccactg tcataaaaaa caaaaatgaa atcgaactaa ataacatact ggcttcctaa
     1321 cctctcctgc tgacgttcta ccagatccgc aacagtcaca tgatccacca catcattgat
     1381 tgcgcattcg agctgcttcc agatggcaag tgtatcacat gtgtcgcatc tttcgcattc
     1441 attcgtctca ccttccaggc atgcaacagg agcaagactg ccttccgtaa gtcttaaaat
     1501 cattcctacc gtatattctt tcggctcctt tgcaagctta tagccgccct gcgcaccacg
     1561 gacgctcttc acatagccgg ctttattcag cactgctatg atctgttcca ggtatttctt
     1621 cgatatttcc tgacggcgcg cgatctgctt taccttaata tattccccgg tattatgaac
     1681 ggcaagatcc agcatcacac gaaccgcgta ccttcctttt gtggatatct tcatcgtaca
     1741 cctcttcttc ccggcttctt acagacagcc tatgctaata tctccaggat ttttgatagc
     1801 cacgtaccac attttatcat atttttcact cttctgtaaa tagcggtgtg gataaataac
     1861 ggtctcccgt atccggcagc agaacaacga tcgtctttcc ttcgttttcc ggtctgcccg
     1921 caatttctgc agccgcatgc agtgccgcac cagaagaaat tcctactaaa atcccttctg
     1981 tcagtgcaat ttcccggcct gccgcaaacg catcctcatt tttcaccgta atgatctcat
     2041 catagatgtc tctgttaaga acctgcggaa taaaacccgc tccaatgccc tgaatcttat
     2101 gcggccccgg cgtccctgca gaaagtaccg gggaatctgc cggttcaatg gcaattattt
     2161 ttatatccgg atttttctgt tttaaatatt tacctgttcc gctaagcgta ccgcctgttc
     2221 caactccagc gacaaaaaaa tcaacagttc cgtccgtatc atcccagatc tccggaccgg
     2281 tcgtcctctc atgagccgcc ggattcgcag gattatcaaa ctgtccaaga atcaccgagc
     2341 cgggaatctg cgctttcaat tcttccgctt tttcgatcgc acccttcatc ccctttgttc
     2401 cgtttgtcaa tacgacctgc gcaccatatg cttttaaaat atttcttctt tccacactca
     2461 ttgtttccgg cattgtcaat attgcatgat atccctttgc agcagctacc gatgcaattc
     2521 cgatgccggt atttccagat gttggctcaa tgatcgttgc ccctgccttt aaaattcctt
     2581 tctgttccgc atcttcgatc attgcaagcg caattctgtc tttcacactg cctgccggat
     2641 tcagatattc cagcttcgca agcactctcg ccttcaatcc gtgcttcttt tcataatttg
     2701 atatttcaag cagaggcgtt cctccgatca gttccgttgt actcgtttta atctttgcca
     2761 tctttagttc ctccttttca tttcctactt atcttataga catattatga attagattat
     2821 actgatgtct tcttactctg tcaacaattt tataaatttc tcttatagca aaatcagtcc
     2881 tgcaaatccg ataccggaaa ccgcaggact gatttctgaa ttttatttta tcataccggc
     2941 tttatttccc ttgttttttc ttctccagac aatgctgtga tcaatacccc cgctataatg
     3001 aaaaaatcag aaatattaaa tacaagattt ttgatttttt ttctttttac attaaaactc
     3061 atataatcga caacatatcc tcttctgatc ctgtcgaatg tattactcaa agcgccgccg
     3121 atcaaacatg caaaaccaag tttgccggcc gtgtttcggc ctttcccggc aaacgtttct
     3181 tccccaagca gtattacaag aacagtgagt cctgcggata cggcagctgt taatttctga
     3241 tatttttcaa aagcattcag catcacacct ttattatggt gcttgcggat aagcagacgg
     3301 tccctccata tttttttcac actgcctgcc ttaacctttt tttcaaccgc actcttgatc
     3361 tgactgtcca cgccaaacaa cagcacagcc agtaaccgat agcccatcct tctcacccgc
     3421 ttttatctgc ctttccataa aaatcatttt gccgcagtca gggacggtgt tcccattgca
     3481 ttcagccgct ccctctcaga aaagctcaga tctccgcccc ttagatcaac cagcggtccg
     3541 cctttcccca atatataatc tttcgtgatc gtcactctgc cgatattttc gtcttttgga
     3601 atctcataca tgatatccag cataaattct tcaatgatcg aacgaagtgc tcttgctccc
     3661 gtatcctttt ccagcgcctt tttggcaatt tcctcaagcg catcctcttc gaattcaagc
     3721 tgtacttcat caag
//
LOCUS       DAOSYE010000096         3716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17457_length_3716_cov_3.438678, whole genome shotgun sequence.
ACCESSION   DAOSYE010000096 DAOSYE010000000
VERSION     DAOSYE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17457_length_3716_cov_3.438678"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLU43_02530"
     CDS             <1..863
                     /locus_tag="PLU43_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO27313.1"
                     /translation="YDRSRYQLDFRNPEVIAYVNGVMDRIITDYGVGYIKMDYNIEPG
                     IGTELYADSVGDGMLGHERAYIAWLKSIFEKYPDLIIENCSSGGLRMDYAMLSQYSIQ
                     STSDQEYYDRYATISANAPAGLTPEQAAVWSYPMREGDKEEVVFNMVNAMLLRIHQSG
                     HLAELSDERKALVKEGLDYYKTIRNSIKTALPFWPLGLSNYEDEWVSLGLLTQDGTNK
                     IYLSVFRRDGGEETCALPISYTKGKKVSVKCAYPSYEECEYCWNESGKQLSVCFPKRN
                     MARLFELTLE"
     gene            complement(869..2041)
                     /locus_tag="PLU43_02535"
     CDS             complement(869..2041)
                     /locus_tag="PLU43_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOO27314.1"
                     /translation="MDKKEYALSMHEKWNGKIEITSRAELNTMSDLAVAYTPGVAEPC
                     LKISEDTELSYKYTRRGNLVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKRFGNVD
                     AFPLCIRSKDVDEIVNTVALLAGSFGGINLEDIAAPRCFEIEQKLKERCDIPIFHDDQ
                     HGTAVVVLAGLINALKLVNKKMSDIEVVVNGSGAAGIAITKLLMKTGLKNVILCDTKG
                     AIYDGREHLNSIKAEMARISNHAKKKGQLADVIKGADVFIGVSAPGVLTPAMVAGMAK
                     QPIVLAMANPVPEIMPDEAKKAGVAVMGTGRSDFPNQINNVLAFPGIFRGTLDVRARD
                     INDEMKLAAAYAIASIISEKDLSPAYIIPDSFNENVTPAVAKAVAKAARETGAARL"
     gene            2501..3646
                     /locus_tag="PLU43_02540"
     CDS             2501..3646
                     /locus_tag="PLU43_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOO27315.1"
                     /translation="MNKFELIAPCHFGLESVLKKEILDLGYDISAVEDGRVYFWGDEA
                     AVCRANIFLRTAERILIKTASFPAKTFDELFEGTKAITWEQYLPQDAKFWVAKAASVK
                     SKLFSPSDIQSIIKKAIVERLKQTYHVTWFSEDGVDYPLRVFLMKDIVTIGLDTTGES
                     LHKRGYRKLVSKAPVAENLAAGMLLLTPWNRDRILLDPFCGSGTIPIEAAMIAAGMAP
                     GMNRSFLAEDWTQLVGRKNWYRTADEASDLVDMTVKPDIQGFDIDGDIIKAAKENARL
                     AGVEEMIHFQKRPVSELNHPKKYGFLVTNPPYGERIGEKEMLPQLYGELAEAYKRLDL
                     WSAYIISAYADVEKYMGRKADKNRKIYNGMMKTYYYQFLGPRPPKRP"
BASE COUNT         1033 a          749 c          952 g          982 t
ORIGIN      
        1 tgtatgacag aagccgctat cagctggact tcaggaatcc ggaggtcatt gcgtatgtaa
       61 acggtgttat ggacaggatc attaccgatt atggggtcgg ttatattaaa atggattata
      121 atattgaacc ggggatcgga acggaactgt atgcggacag tgtgggtgac ggcatgctcg
      181 gacatgaaag agcatatatt gcgtggctga aatccatatt tgaaaagtat ccggatctga
      241 tcattgaaaa ttgttccagc ggagggcttc ggatggatta tgccatgctt tcccagtaca
      301 gcatccagtc gacgagtgat caggaatact atgacagata tgcgacgatc tcagcaaatg
      361 cgccggcagg actgacaccg gagcaggccg ccgtctggtc gtatccgatg cgtgaaggag
      421 ataaggaaga ggtcgtattt aacatggtca atgcgatgct tttaagaatt catcaaagcg
      481 gacaccttgc ggagctttcc gatgaacgga aggcgcttgt gaaagaagga ctggattact
      541 ataaaacgat tcgaaacagc atcaaaacag cgcttccctt ctggcctctc gggctttcaa
      601 attatgaaga tgaatgggtg tcacttggac ttctgacaca ggacggtacg aataagatct
      661 atctttcggt ctttcgccgc gatggcggcg aagagacctg tgcgctgccg atctcctata
      721 caaaagggaa gaaggtaagc gtgaaatgtg catatccttc ttacgaagaa tgcgaatatt
      781 gctggaacga gtccggtaaa cagctgtccg tatgttttcc gaaacggaat atggcaaggc
      841 tgtttgaact tactctggaa tgatcgagct ataatctggc cgcaccggtt tcccttgcgg
      901 cttttgcaac agcctttgca acagcaggcg ttacattttc attaaaactg tccggaatga
      961 tataagcagg agaaaggtct ttttcagaaa taatggaagc gatcgcatag gcggcggcca
     1021 gtttcatttc gtcattgatg tccctggcac gtacatccag ggttccgcgg aaaatgccgg
     1081 ggaatgcaag gacattattg atctgattcg ggaagtcgct ccttccggtt cccataacgg
     1141 cgacaccggc ctttttggcc tcgtcaggca tgatttccgg aacgggattt gccatggcaa
     1201 gaacaatcgg ctgttttgcc attcctgcta ccattgcagg cgttaagacg cctggtgcgg
     1261 atacgccgat aaagacgtcg gcgcctttta tgacatcggc aagctgtccc tttttctttg
     1321 catgatttga gatccgtgcc atttcggctt tgatgctgtt caaatgttcg cggccgtcat
     1381 aaatagcacc ctttgtgtcg cataatataa cgtttttcag cccggtcttc atcagcagtt
     1441 tggtaatggc aatgccggca gcaccggagc cgtttacgac aacttcgata tcggacattt
     1501 ttttatttac aagctttaaa gcattgatca ggccggcaag tacaacaact gccgtaccgt
     1561 gctggtcgtc atgaaagatc ggaatgtcgc agcgttcttt taatttttgt tcgatttcaa
     1621 agcatctggg tgcggcaata tcttcaagat tgatgccgcc gaaagaaccg gcaagcaaag
     1681 caacggtatt gacgatctca tctacgtcct tggaacggat gcagagcgga aacgcgtcta
     1741 catttccgaa gcgcttaaaa aggacacatt ttccttccat aacaggcata ccggcctccg
     1801 gaccaatatc accaaggccg agtacggcag ttccgtccgt tacgaccgca acgaggttgc
     1861 cgcggcgtgt atatttatag gatagttccg tatcctcgct gattttcaga cagggttctg
     1921 cgacgcccgg tgtatatgcg actgccagat ccgacattgt atttaattcg gctctgcttg
     1981 tgatttcgat cttaccgttc catttctcat gcatgctcag tgcatattct tttttatcca
     2041 tttgaattcc tccatgctta ttaatgtcaa aacaatcttt tctgattata gcatgattgc
     2101 atctgttcgt aaaatggatt tcaattttca cggataaggg agctgtctgg aattgagaaa
     2161 gaaaacgaat ggaaataaaa agtattgtag cccgatgggg gaaccaacgg gctacgatga
     2221 ggggagtata aataattaat aaggggtcgt aaatgagaat cctttgaagg gtagatcctc
     2281 gcttacatta gtaattataa tataatccga taaaaaatac aatagatttt gaaaactagt
     2341 agttataaat atttaaataa caatatttag tgttttttca tgaaaattgt attaaaaaaa
     2401 ggagaaatct gtgttttttt ataaaaaata ttcgctaaag aaagtaatga ttgacaccat
     2461 tcgccggcta ttttaatata attgggaagg caggtacaat atgaacaaat ttgagttgat
     2521 tgctccatgc cattttgggc tggagtccgt attaaaaaaa gaaattctgg acttaggata
     2581 tgacatttcg gcggttgaag acggcagagt gtatttttgg ggtgatgaag cagctgtttg
     2641 ccgtgcgaat atttttttgc gcactgcaga gcgtattttg atcaaaacgg cttcttttcc
     2701 ggcaaaaacg tttgatgaac tatttgaagg gacaaaggcg atcacatggg aacaatatct
     2761 gccgcaggat gcaaaattct gggtggcaaa agcggcaagt gtgaaaagta agctgttcag
     2821 tccgtccgat atccaatcca taataaaaaa ggcaatcgtg gaaagactga agcagactta
     2881 tcatgttaca tggttttcag aggacggtgt ggactatcct ctccgtgttt ttctgatgaa
     2941 ggatatcgtt acgatcgggc tggatacaac aggggaatcg ctgcataaga gaggttacag
     3001 aaagcttgtc agcaaagcgc cggtagcaga aaatctggcg gcaggcatgc ttttgctgac
     3061 gccgtggaat cgggatcgta ttttgttaga tccgttctgc ggcagcggaa cgatcccgat
     3121 cgaagcggca atgatcgctg cgggtatggc gcccggaatg aacagaagtt ttctggcgga
     3181 ggactggaca cagctggtag gcagaaagaa ctggtaccgg acagccgacg aggcgtctga
     3241 tctggtcgat atgactgtga aaccggacat tcagggattt gatatcgacg gggatatcat
     3301 taaggcggca aaggaaaatg ccaggcttgc cggcgtcgaa gaaatgatcc attttcagaa
     3361 gcgtccggtc agtgaattga atcatccgaa gaaatacggt tttcttgtga caaatccgcc
     3421 gtacggggaa cggatcggag aaaaggagat gcttccgcag ctttacggag agctggcaga
     3481 agcgtataag cgtctggatc tctggtcggc atatatcatt tccgcctatg cggatgtgga
     3541 aaaatacatg ggacgcaagg cggataaaaa cagaaagatc tataacggga tgatgaaaac
     3601 gtattattat cagtttttag ggccgaggcc ccctaaacgg ccataaacag aagctgcagc
     3661 aatgaacgga cagctgccgt gaaaggtgtt tcttcttatt ataatgcggc aatagg
//
LOCUS       DAOSYE010000097         3714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17477_length_3714_cov_2.685980, whole genome shotgun sequence.
ACCESSION   DAOSYE010000097 DAOSYE010000000
VERSION     DAOSYE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17477_length_3714_cov_2.685980"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLU43_02545"
     CDS             <1..1085
                     /locus_tag="PLU43_02545"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOO27316.1"
                     /translation="GNTVEQIITKENFVAYFGGDTFLAVVNDSNDKRMEELLKKINER
                     LKNSWVIDGQEFKISMSIGAAVYPVDGKDTGSLLKNADLAMYKAKENGGAQTYICSQA
                     MREEVEKKQIITNYLYKAIERKEIYLVFQPKVNASSEKIEAVEALVRWNNKEIGMIPP
                     AVFIPLAERTGIINQIGEWVFEEACRQSRRWKEMGKKLIPIAVNVSGYQLQDVKIISK
                     MKHIILENMVDPKFIEIEVTESIAMNLAGDVSELLFQLKKIGISIAIDDFGTGYSSLS
                     RLNELPIDVLKIDKKFVDGILKSDRERAMIKTIIDLAKNLNLKIVAEGAETKEQVDFL
                     KQKQCDFIQGYYYFKPLSVEELNTYL"
     gene            complement(1086..1913)
                     /gene="amrS"
                     /locus_tag="PLU43_02550"
     CDS             complement(1086..1913)
                     /gene="amrS"
                     /locus_tag="PLU43_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO27317.1"
                     /translation="MKAKCEICPHHCILDEGQAGLCKGRRNQNGRIISDNYGKLTAAA
                     LDPIEKKPLRRFYPGSMIFSVGSYGCNLGCPFCQNYEISTVGGSEIETAAFTCEELIH
                     KAVMQKNKNNIGIAYTYNEPLIGYEFVRDCAVLAKQAGLKNVVVSNGYICEEPLKELL
                     PVIDACNIDLKGFTENYYHMLRGDLSTVKRSIEIAAAACHVETTTLIIPGENDSEEEM
                     ENLSSWLANISPAIPLHISRFFPMWKMQDRGPTPVKQVYHLADVARKHLKYVYEGNV"
     gene            complement(1910..3295)
                     /gene="amrA"
                     /locus_tag="PLU43_02555"
     CDS             complement(1910..3295)
                     /gene="amrA"
                     /locus_tag="PLU43_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOO27318.1"
                     /translation="MSLLGAFIVPHPPLIIPEVGKGEEEKISDTVNSYLEISKHIAAL
                     KPDTIILTSPHSILYADYFHISPGKSARGDFGRFGAGKITISAEYDAEFAEALGWAAE
                     KAGIAAGPLGERDAALDHGTMVPLYFINQFLSGYKLVRIGLSGLSLCDHYSLGRCIAK
                     TAEKSGRKIIFVASGDLSHKLKEDGPYGFAKEGPKFDKEVTDIIKTGDFLKLLEISPN
                     LCEAAGECGLRSFVIMAGSLSGKAVEPKLLSYEGPFGVGYAVGEFKITGEKKEQRFDE
                     IFAEKQKKLARERQEDEDAYVSLARLSLETYVNTGKYAAFPQNLPEEFLKKKAGVFVS
                     LKKFGNLRGCIGTIAPVSPNIAEEILRNAVSAGTEDPRFPPVTKTELEELIYSVDVLE
                     KPEPIAHMTELDVKRYGVIVTSGRKRGLLLPNLEGVDTVEQQVSIAKRKAGIRDNEHF
                     TMERFEVVRHQ"
     gene            complement(3325..>3714)
                     /locus_tag="PLU43_02560"
     CDS             complement(3325..>3714)
                     /locus_tag="PLU43_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27319.1"
                     /translation="YDENVFRLDYYGTIAEVEQYVTEHLSGELTLESIAKQVYLNPSY
                     LSTMYHKSTGSTLFKFIQKERISKSLYLLCNSTMSIKEISEAVGFKDSNYFSKVFVRE
                     NGCSPRQYLKKVAKGEILQGDLQNEPV"
BASE COUNT         1030 a          781 c          812 g         1091 t
ORIGIN      
        1 tcggaaatac agttgaacag atcataacaa aagagaattt tgttgcatat tttggtggag
       61 atacgttcct tgctgttgta aatgactcca atgacaaaag gatggaagag ttgttgaaaa
      121 aaattaatga aaggctgaag aattcttggg tgattgatgg tcaggagttt aaaatatcaa
      181 tgagcatagg agccgcagta tatcctgttg atgggaaaga tacagggagc ctgctcaaaa
      241 atgcagattt ggcaatgtat aaagcaaaag aaaatggagg cgcacagacg tatatctgtt
      301 ctcaggcaat gagggaggaa gtagagaaaa aacaaataat tactaattat ttgtataagg
      361 caattgaacg aaaggagata tatttggtct ttcagccaaa agtaaatgct tccagcgaaa
      421 aaatagaagc agtggaagcg ctggtgcgat ggaataataa agaaattggt atgattcctc
      481 ctgcggtttt tatcccgctt gcggaaagaa ccggtattat aaaccagatc ggcgaatggg
      541 tgtttgaaga agcctgcagg cagtcacgga gatggaagga aatgggcaaa aaactgatac
      601 cgatagcagt caatgtatca ggatatcagt tacaggatgt aaaaataatc agtaaaatga
      661 aacatataat tttagaaaat atggtagacc caaagtttat tgaaattgaa gtgacagaga
      721 gtattgcgat gaatttggca ggtgatgttt ctgaactgct gtttcagcta aaaaaaattg
      781 gcattagtat tgcaatcgat gattttggaa caggatattc atctttgagt cggttgaatg
      841 aactgccgat cgatgttttg aaaatagata agaagttcgt tgatggtata ctgaaatcag
      901 atagagaacg ggccatgatt aaaacgatta tagatcttgc caagaatctg aatctgaaga
      961 ttgtagccga aggagcagag acaaaggaac aggtcgattt tttaaagcag aaacaatgtg
     1021 attttataca gggctattat tatttcaaac cgctgtctgt tgaggaatta aatacgtatt
     1081 tatgatcaca catttccctc atacacgtat tttaaatgct ttcttgccac gtctgcaaga
     1141 tgatacacct gctttaccgg tgtcggtccc cggtcctgca ttttccacat agggaaaaag
     1201 cgggagatgt ggagcggaat agcagggctg atatttgcca gccagctgct taagttttcc
     1261 atttcctcct cactgtcgtt ttcgccggga ataattaagg tcgtggtctc aacgtggcag
     1321 gccgctgctg cgatctctat cgaacgtttt acggtagaaa gatctccgcg cagcatgtga
     1381 taatagtttt ccgtaaatcc tttcagatca atgttgcatg catctatgac aggcaggagt
     1441 tccttcagcg gctcttcgca gatatatcca ttggatacga caacgttttt cagtcctgcc
     1501 tgttttgcca ggaccgcaca gtcccggaca aattcataac cgatcagcgg ttcattatag
     1561 gtgtaggcaa tgccgatatt atttttgttt ttttgcatga ctgctttatg gatcagttcc
     1621 tcgcacgtaa atgcggcggt ttcgatttct gaaccgccga ccgtggagat ctcatagttc
     1681 tgacaaaatg gacagcctaa attacagcca tagcttccaa ccgaaaatat catgctgccg
     1741 ggataaaatc tccgcagcgg ttttttttcg atcggatcaa gcgccgcggc cgtcagtttt
     1801 ccataattgt cactgatgat ccggccattt tgatttcgtc tgcctttgca caggccggcc
     1861 tgtccttcgt ccagtataca gtgatgggga cagatttcac attttgcttt cattgatgtc
     1921 tcaccacctc aaaacgttcc attgtaaaat gttcgttatc acggatgcct gcttttcttt
     1981 tagcgatgga gacctgctgt tctacggtat ccactccttc cagattcggc agcaggagcc
     2041 cccgttttcg tccggaagtt acgatgacgc cgtaacgttt cacatcaagc tccgtcatat
     2101 gtgcgatcgg ttccggtttt tcaaggacat ccacactata gatcagttct tccagttccg
     2161 tctttgttac gggaggaaat ctgggatcct cagtgcctgc gcttacggcg ttgcgcaata
     2221 tttcttcagc aatatttggg gagaccggcg caattgttcc gatgcatccg cgcaaatttc
     2281 caaatttttt taaagacaca aaaacgcctg cttttttctt taagaattcc tccggcaaat
     2341 tttgcggaaa cgctgcatat ttaccggtgt ttacataggt ttccaaagag agtctcgcaa
     2401 gactgacata ggcatcctca tcctcctgcc gttcccttgc cagcttcttc tgtttttccg
     2461 caaatatctc atcaaagcgc tgttctttct tttcccctgt aattttgaat tctcctacgg
     2521 catagcctac accaaatggt ccctcataag ataaaagttt tggctccact gctttgccgg
     2581 acagagaacc tgccataatg acaaacgatc ttaaaccgca ttcgcctgct gcttcgcata
     2641 aatttggaga tatttcaagc agcttcagaa aatctcccgt ttttataata tccgttactt
     2701 ctttatcgaa tttcggtccc tctttggcaa aaccatacgg tccgtcttct tttaatttat
     2761 gggaaagatc accgctggct acaaaaatga tttttcgtcc tgatttttcg gcagtttttg
     2821 caatacatct gccaaggctg tagtgatcgc aaagtgacaa gccggataaa ccgatccgta
     2881 ctaatttata tccggataaa aactggttga taaaatacag aggtaccatc gtaccgtgat
     2941 caagggcggc atcccgctct cctaaaggac cggccgcaat acctgctttt tctgctgccc
     3001 atcccagagc ttctgcgaat tccgcatcat attctgcgct gatcgttatc ttccctgcac
     3061 caaaacggcc gaaatcaccc ctggccgatt tcccgggaga aatatgaaaa taatccgcat
     3121 acagaatgga atgcggtgag gtaagaatga tcgtatccgg tttcagcgct gcaatgtgtt
     3181 ttgagatttc aagatagctg ttaacggtat cactgatctt ttcttcttcc ccttttccga
     3241 cttccggaat aataagcggc ggatgcggta caataaatgc tcctaaaagt gacatatgat
     3301 acacctccat aagttttgaa acgtttatac aggttcattt tgcagatctc cctgcaggat
     3361 ttcccctttg gcgacttttt tcagatactg ccggggagag cagccgtttt ccctgacaaa
     3421 gactttgctg aagtagttgg aatctttaaa tccgacagct tcggatattt ccttaatgga
     3481 catcgtactg ttgcagagta aatagagaga cttgctgatg cgttctttct ggatgaattt
     3541 gaataaagtg ctgcctgtgg acttgtgata catggtgctg agataagaag gattcaaata
     3601 gacctgcttt gcaatacttt ccaaagtcag ttcaccggaa agatgctcgg tcacgtattg
     3661 ttccacttcg gctatagtgc cataatagtc aagtctgaat acattttcat cata
//
LOCUS       DAOSYE010000098         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17738_length_3676_cov_3.338857, whole genome shotgun sequence.
ACCESSION   DAOSYE010000098 DAOSYE010000000
VERSION     DAOSYE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_3676_cov_3.338857"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLU43_02565"
     CDS             complement(<1..863)
                     /locus_tag="PLU43_02565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO27320.1"
                     /translation="MEGFRAVDLVIYAECILIYIIINIHRSGKRKERSIRLFLYCVYS
                     LLVVNMLEGVTWAINGLIYPGGRQISFITNTVLLLCNSLPAVTWVAYADNKIFSNAKK
                     LKKRVRLYLIPFYMTAVLLILNIWNGFVFTIDENNVYARGPGVYIISSMIYILVICLY
                     FATRKYKKQINGKITQSILLFMLFPVVGGIIQMFAYGVLLVWPSFIFASLIAFFQIER
                     EEILCDPLTGLTLREQMEQRAQYLMNKKIAFFVIMIDMDGFKSINDQYGHHEGDLALI
                     TAASFLRNSVR"
     gene            complement(998..2734)
                     /locus_tag="PLU43_02570"
     CDS             complement(998..2734)
                     /locus_tag="PLU43_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO27321.1"
                     /translation="MGDNKSPASKSGRKMLSHKIAVIVYLLIYFSSFFFLMKISYIDF
                     VYMFGIKINAVVINGVITQIQIMAMIGFITSAAMKKRFLLAHIFNASFFLSSAILVVF
                     FHLEGAVPGFAIAVSLYIIIMLIQKYDARIMLSLQDVQKKNEQLQKMVYFDIMTGLPN
                     RQMIVNRMNFLIQNPYEEQDSFAVVMIDLNNFKQINDLYGHSMGDKFINKTAQILKEF
                     VDERDILGRLVGGDEFILVIQRRLKGSELYQYVDHLRDYLCSSVLDDELKLSISASFG
                     VSTYPEDAQDPEQLLKYADIAMYSAQKAGRNAIEFFNRSLYTTLKQSVAFEQNLKTAV
                     SNEEMFLNFQPQYFAKTKRLRGFEVLLRWTSAQYGFISPAKFIPIAEETGLIVPIGEW
                     VFYQSCKKLAQIMQMPACRNIVMSVNLSVVQIMESGFMQMIDRVLKETKVSPANLEVE
                     ITESVFISSADYVMNILNQIKQRGIRISLDDFGTGYSSLNYLQMLPIDVVKIDKSFVD
                     CIGGMEAQSKIVGSLIELFHRLDLSVVAEGVENEMQKDYLVENACDYIQGFLWGKPLL
                     EKEADELLLQQG"
     gene            complement(2753..3292)
                     /locus_tag="PLU43_02575"
     CDS             complement(2753..3292)
                     /locus_tag="PLU43_02575"
                     /inference="COORDINATES: protein motif:HMM:NF023134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOO27322.1"
                     /translation="MEFINSGWYNQHERFIEVLKDDAFIKEYCEKWELPVPEFNDHSV
                     TKLLHVRKELQRIALKVCHDQTLLPEDFDMINSYLSLGVYRQELVMENESFQINTIPK
                     SSALDWLIYKIAFSFVDLIVHYRLDNLKECENPDCDWIFYDTSKNHSRKWCENKCASL
                     IKVRRFREVQKEKNQQNKQ"
     gene            3437..>3676
                     /locus_tag="PLU43_02580"
     CDS             3437..>3676
                     /locus_tag="PLU43_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO27323.1"
                     /translation="MILLLATIIWGTSLVTQSIGMSYIGPFTFNAVRFFVGAATLIPI
                     IALKGRKGKAILSINVKKTTLSAGIINGTVLFLTAS"
BASE COUNT         1167 a          851 c          616 g         1042 t
ORIGIN      
        1 tcccgcacgc tgtttctcaa aaaagaggct gccgtaatga gcgccagatc accctcatgg
       61 tgtccgtact gatcattaat acttttaaat ccatccatat cgatcatgat cacaaaaaaa
      121 gcgatctttt tattcatcag atactgtgca cgctgctcca tttgttcccg cagcgtcaga
      181 ccggtcagcg gatcacataa tatttcttcc ctctctattt gaaaaaatgc aataagcgac
      241 gcaaaaataa aggaaggcca tacaagcaaa acgccatatg caaacatctg aataatgccg
      301 cccactaccg gaaacagcat aaaaagaagg atagactgcg tgatctttcc gtttatctgt
      361 ttcttgtatt ttcttgtcgc aaaatacaga cagatgacca gaatataaat catcgatgag
      421 ataatatata cacccgggcc tcttgcatac acattatttt catcgatcgt aaaaacaaac
      481 ccgttccata tattcaatat aagaagaacg gctgtcatat aaaatgggat cagataaagc
      541 ctgacccgtt tttttaactt tttcgcattg ctgaaaattt tattatccgc ataagccacc
      601 cacgtaacgg ccggaagcga attgcacagc agcagtaccg tatttgtaat aaaactgatc
      661 tgacgtccgc ccggataaat cagtccgttg atcgcccaag tgactccctc cagcatatta
      721 acgaccagaa gagaatacac acaatacaaa aataaacgaa ttgatctttc cttccgcttg
      781 ccggaacgat gaatattgat gatgatatat ataaggatac attctgcgta tataaccaag
      841 tcaactgccc gaaaaccttc catttctccc gcgctcctat aaacccaact gccgctgcaa
      901 aatgatcccc ttattttata taagctcccc tatttcataa tacgattctg ttagaaaggc
      961 gtcaagatac tgcctgtaaa aaataaattt caatacgcta tccctgctgc aacagcagtt
     1021 cgtcggcttc cttctccaaa agtggtttcc cccataaaaa accctgaata taatcgcagg
     1081 cattttcaac cagatagtcc ttttgcattt cattttccac gccttctgcg acaacagaca
     1141 gatcaagccg gtgaaacaat tcgatcagag aaccgacgat cttgctctgt gcttccatgc
     1201 cgccaataca atctacaaaa cttttatcga tcttaacaac atcgatcggc agcatctgca
     1261 gataattcag cgaagaatag ccggtaccga aatcatccaa agaaatgcgg atcccccgtt
     1321 gtttgatctg gttcaggata ttcataacat agtcggccga tgagataaac acggattcag
     1381 tgatctcaac ctccagatta gccggactga cttttgtttc cttaagaacc ctgtcgatca
     1441 tctgcatgaa tccgctctcc atgatctgaa cgacagagag gttcacagac atcacaatat
     1501 tccgacaggc aggcatctgc atgatctgag caagcttttt acagctttga taaaaaaccc
     1561 actcgccgat cggaacgatc aaacctgtct cctccgcaat cggaatgaat tttgcaggac
     1621 tgataaaacc atactgtgcg gatgtccagc gcagaagcac ttcaaatcct cgcagacgct
     1681 tggttttggc aaaatattgc ggctgaaaat tgagaaacat ttcttcattg gaaacagctg
     1741 tttttaaatt ctgttcaaaa gcaacgctct gctttaatgt cgtatagaga gacctgttaa
     1801 aaaactcgat cgcgtttcgt cctgctttct gagcactgta catcgcaata tcagcatatt
     1861 tcaaaagctg ttccggatcc tgcgcatctt ccggatacgt tgaaacgcca aaactggcac
     1921 ttatcgacaa tttcagctca tcatcaagca cagaagaaca caaataatca cgcagatggt
     1981 ccacatattg ataaagctcg cttcccttga gtcttctctg gatgaccaga ataaattcgt
     2041 cgccgcctac aagacgtccc aatatatcac gttcatcaac aaactccttc aaaatctgcg
     2101 cagtcttatt aataaattta tcgcccatgg aatgtccgta cagatcattg atctgtttga
     2161 aattattaag atcgatcatt acaacggcaa atgaatcctg ttcttcatag ggattttgaa
     2221 tcaaaaaatt catccggttt acgatcatct gcctgtttgg aaggccggtc ataatatcaa
     2281 aatataccat cttctgcagc tgttcattct ttttttgaac atcctgcagg gaaagcatga
     2341 ttctcgcatc atatttctga ataagcatta taataatata aaggctgacc gctatggcaa
     2401 accccgggac agctccctcc aaatggaaaa aaacaacaag aatcgcactt gacaggaaaa
     2461 atgaagcatt aaatatatga gcgagcagaa accgcttttt catcgcagca ctcgtaataa
     2521 atccgatcat ggccatgatc tggatctgcg taatgactcc gttgattaca acagcattga
     2581 tctttatacc aaacatgtaa acaaaatcga tgtatgaaat tttcatcaga aagaaaaaag
     2641 aactaaaata gatcagcagg taaacaatca ctgctatttt atgagacaac atttttctcc
     2701 ctgattttga tgccggactt ttgttatcac ccatgatgcg taacttttcc tttcactgct
     2761 tgttctgctg attcttttct ttttggacct cgcgaaatct tcgtactttt atcagacttg
     2821 cacatttatt ttcacaccat tttctggaat ggtttttgct cgtatcataa aaaatccagt
     2881 cacagtccgg gttttcacat tctttcaaat tatcaagccg ataatgcacg atcaaatcaa
     2941 caaatgaaaa agcaatttta tagatcagcc agtccagtgc actgcttttc ggaatcgtat
     3001 tgatctgaaa actctcattc tccataacaa gctcctgccg ataaacacca agagacagat
     3061 aggaattgat catatcaaaa tcttccggca gcagcgtctg atcatggcat acctttaaag
     3121 caatacgctg aagctccttc cggacatgga gtaattttgt aacagagtga tcgttaaact
     3181 ccggtaccgg cagttcccat ttttcacaat attcttttat aaaagcgtcg tctttcaata
     3241 cttctataaa ccgctcatgc tgattatacc agccgctgtt aataaattcc aaacatagaa
     3301 attccaaaag atcaccttct taaatcatat tgacaattac ttctatatcg tatatggtaa
     3361 attgtaacga tctatattta ttttaacggt tacaactaaa ggaggaaaca atttgcgcaa
     3421 cagatttaaa ggaagtatga ttctgctcct tgcaacaatt atatggggaa catcattagt
     3481 tacacaaagc attggtatgt cttatatcgg accatttacc tttaatgccg taagattttt
     3541 tgtcggtgca gccacattaa ttcccattat agcattaaaa ggaagaaaag gaaaagcaat
     3601 actttcaata aatgtaaaaa aaacaacact gtctgcagga attataaacg gtaccgtact
     3661 gtttttgaca gcctct
//
LOCUS       DAOSYE010000099         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17739_length_3676_cov_3.264844, whole genome shotgun sequence.
ACCESSION   DAOSYE010000099 DAOSYE010000000
VERSION     DAOSYE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17739_length_3676_cov_3.264844"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLU43_02585"
     CDS             complement(<1..543)
                     /locus_tag="PLU43_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOO27324.1"
                     /translation="MIFALVGNQNCGKTTLFNALTGSNQHVGNFPGVTVDQKMGEMKY
                     VKDCSVVDLPGIYSIRPYTTEEIVTRDFVLNQKPDGIINIIDATNIERNLYLSLQLLE
                     LHIPMVIVLNMMDEVRNNCGSINVREMSVRLGVPVIPISAAKNEGISEVAYKIEEVSR
                     KKILPKIMDFCPGGAVHRCIH"
     gene            complement(540..776)
                     /locus_tag="PLU43_02590"
     CDS             complement(540..776)
                     /locus_tag="PLU43_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOO27325.1"
                     /translation="MTINDLKIGEEGVIREVLGEGVLRLRFLDMGLIPRTKVRVQKIA
                     PLGDPIQLSIRGYELTIRREDAGKIMIESEASDR"
     gene            complement(1014..1586)
                     /gene="scpB"
                     /locus_tag="PLU43_02595"
     CDS             complement(1014..1586)
                     /gene="scpB"
                     /locus_tag="PLU43_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO27326.1"
                     /translation="MPVENPKAVMEAILFTMGNSVEIKKLAGVIGRDEKETKELLHEM
                     QAAYDSDSDRGISIMELENAVQMCSKKEMYEHLVKVAAAPKNFVLTDTLLETLSIVAY
                     KQPVTRLEIEKIRGVSCDHAVNRLMEYGLIMELGRLDAPGRPLLFGTTEEFLRSFGVK
                     SIEELPMAAPERLEEFKKEAAEEVQLKLDI"
     gene            complement(1719..2468)
                     /locus_tag="PLU43_02600"
     CDS             complement(1719..2468)
                     /locus_tag="PLU43_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOO27327.1"
                     /translation="MGLTVKLQVFEGPLDLLLHLIEKNKVDIYDIPISLITDQYLEYI
                     RQMETEDLNIMSEFLVMAATLLDIKCRMLLPKETDEDGEEEDPRAELVEKLIEYKLYK
                     YMSFELKDRQIDAVKALFKKPTFPQEVAEYEQPIDYEKLVGDVDLSRLNQIFKSVMKK
                     QEDKIDPLRSQFGKIEKDEVNLEEKTAYIKNYVQAHKRFSFRNLLEEQTSKMEIVVTF
                     LVVLEMMKTGMIMIIQEELFDDIVITSQIAA"
     gene            complement(2483..3349)
                     /locus_tag="PLU43_02605"
     CDS             complement(2483..3349)
                     /locus_tag="PLU43_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO27328.1"
                     /translation="MNVLIIIGILLAVILVCILVFKESSGFTVTNYEFFSDKIKKEKF
                     KIVLLADLHDTDFGNENAVLLQEIDKLDPDIVAFAGDMVTSCMEAEYDYEPTLRFIGR
                     LAAKYPVYYGMGNHEERFRRRPDRFPGKYEDLTKKLRAAGAPILVNEKKEIPDAGVMI
                     YGLDMEHQYYRKIKTCYIPDDYLTRIFGPLDADHVSILLAHNPEQFPKYAKWGPDFVF
                     SGHVHGGVIRIPFLGGLVSPQLKLFPKYDAGEFKEGRSTMILSRGIGSHTTPIRILNK
                     AEIVNIIIRKKK"
BASE COUNT          981 a          957 c          577 g         1161 t
ORIGIN      
        1 atgaatacag cggtgcacag ctccgcccgg acaaaaatcc atgatctttg gcaggatctt
       61 ttttcgtgat acttcttcga tcttatatgc aacttcggaa attccttcgt ttttagccgc
      121 actgatcgga ataaccggca ctccaaggcg cacactcatt tcccgtacat ttatggaacc
      181 gcagttattc cttacttcat ccatcatatt caatactatg accattggaa tatgcagttc
      241 cagcagctgc aggctcaaat aaagatttct ctctatatta gttgcatcaa tgatattgat
      301 gataccgtct ggtttctgat ttaacacaaa atctcttgtg acgatctctt ctgttgtata
      361 aggtctgata gaataaatac ccggcagatc gacaacggaa cagtccttga catacttcat
      421 ctcccccatc ttctggtcta ccgttacccc cggaaagtta ccgacatgct gattagaacc
      481 ggtcagtgca ttgaacagcg ttgtcttccc gcaattctga tttcctacta atgcaaatat
      541 catctgtcag atgcctcact ttctatcatg atctttccag cgtcttctcg tctgatcgtc
      601 aattcatacc cccgaataga aagctgtatc ggatcgccga gcggtgcgat cttctgcacc
      661 ctgacttttg tcctcggaat cagtcccata tccagaaaac gcagccgcag cacaccttct
      721 cccaatactt ccctgataac accctcttct ccaatcttta aatcattgat cgtcataatt
      781 aattttctcc cggctgtcga tttacgcata tttaatgata tagattatca tttgttagcc
      841 tttgctaact aataatagca cattcaatca tagattccaa gcggcatttc aaacagtaaa
      901 atatcttttt ttactgcttt tattgatcat ttttatcttt tctgacgccg gacataaaaa
      961 aactttgcaa atccacctgt tttaagcggt tttgtaaagt cttcatcatt acattaaata
     1021 tccagcttta actgcacttc ttcagctgct tcctttttaa attcttccag ccgttccggt
     1081 gctgccatag gaagctcctc aatggatttc actccgaaac ttctaagaaa ttcctctgtc
     1141 gttccaaata agagaggcct gcccggcgca tccaatcttc cgagttccat gatcagccca
     1201 tactccataa gcctgtttac cgcatgatca cagctgactc cccggatctt ttcaatttca
     1261 agtcttgtaa ccggctgttt atatgccaca atagaaagcg tttccagcag cgtatccgtc
     1321 aatacaaaat tcttcggcgc agccgccact ttaaccagat gttcatacat ctctttcttg
     1381 gaacacatct gtacggcatt ttccagttcc ataatagaaa tacccctgtc actgtcactg
     1441 tcataagcag cctgcatttc atgaagcaat tctttcgttt ctttctcgtc ccggccgatc
     1501 acacctgcca gtttctttat ttccacggaa ttccccatcg taaaaagaat cgcttccata
     1561 acagcctttg gattttctac cggtataaca gattccattt ccactttttc atttgattcc
     1621 ctgatctctt ctttctctga cactctttca aaccccgctg tcctctcata ttgactacag
     1681 atatttataa acccggaccg ataactgttt attttcagct acgccgctat ctgtgatgta
     1741 attacaatat cgtcaaacag ttcctcctgt ataatcatga tcatgcctgt cttcatcatt
     1801 tccagtacca caagaaatgt cactacgatt tccattttgg aagtctgctc ctccaaaaga
     1861 tttctgaaac tgaaacgctt atgtgcctgc acataatttt ttatataagc agtcttctct
     1921 tccagattga cttcatcctt ttcgattttc ccaaactgac ttctgagcgg atcgatctta
     1981 tcttcctgtt tcttcattac agacttaaat atctgattca gcctggacag atccacatca
     2041 cccacaagct tttcatagtc tatcggctgc tcatattccg caacctcctg cggaaatgtc
     2101 ggcttcttga ataacgcctt gaccgcatcg atctgcctgt cctttaattc aaaagacata
     2161 tacttataga gcttatattc gatcagtttt tcaaccagct ctgctctcgg atcttcttct
     2221 tcgccgtcct catccgtctc tttcggaagc agcattctgc atttgatatc caaaagagtc
     2281 gcagccataa caagaaactc gctcataata ttcagatcct cggtttccat ctgcctgata
     2341 tattccagat actgatccgt aatcaatgaa atcggaatat cataaatatc tactttattc
     2401 ttttcaatca gatgcagcaa cagatcaagc ggtccttcaa aaacctgtag ctttactgtt
     2461 aaacccatat gtttcccctc ttttactttt tctttcttat tattatgttt acgatctccg
     2521 ctttattcaa aatgcggatc ggcgtcgtat gcgaaccgat tccacggctt aagatcattg
     2581 tagatcttcc ctctttgaat tccccggcat catatttcgg aaacagtttt agctgcggag
     2641 atacaagtcc tcccaaaaaa ggaatccgaa tcacaccgcc gtgcacatgc cctgaaaaca
     2701 caaaatccgg tccccacttc gcatactttg gaaactgttc cggattgtgt gccaaaagga
     2761 tcgagacatg atccgcgtcc aaaggtccga aaatcctggt cagataatcg tccggaatat
     2821 aacaggtctt tatcttccga taatattgat gctccatatc cagtccgtag atcatgaccc
     2881 cggcatccgg tatttctttt ttctcattaa caaggatcgg tgccccggca gcacgcagct
     2941 tttttgtcag atcttcatac ttgcccggaa acctgtccgg tcttctcctg aaacgttcct
     3001 catgattccc catcccgtaa taaaccggat actttgcggc aagacggccg ataaagcgaa
     3061 gcgtcggctc ataatcatac tccgcctcca tgcacgatgt taccatatct cctgcaaatg
     3121 cgacaatatc aggatccagt ttatcgatct cctgaagcag tacagcattc tcatttccaa
     3181 aatccgtatc atgcagatct gccaatagta cgatcttaaa cttttctttt tttattttat
     3241 cagaaaaaaa ctcataattt gtaacagtaa atccgctgct ttccttaaat actaaaatac
     3301 ataccagtat gacagccaat aggatcccta tgattatcaa tacattcata aatcccccgg
     3361 atcttcacag catcggccgt gttatgaaac gttttattat cactacaata tgttgtatta
     3421 taccataata tttttcataa ataaagtatt ttatcgcaaa ttatactgtc actcaaatat
     3481 gtataaaaac ggcagatact tcacctgttt attgggcagt atcatgggaa agaactccgt
     3541 caaaaaacgg aagcaggtga tccttcatga aatccggcgg taaaatatgt ccgcccgtaa
     3601 acggatagct tgtataggaa attccctctt catcaagcag tccggccaga tattgtgtcc
     3661 cttccggtat ccagct
//
LOCUS       DAOSYE010000100         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17787_length_3670_cov_3.222407, whole genome shotgun sequence.
ACCESSION   DAOSYE010000100 DAOSYE010000000
VERSION     DAOSYE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17787_length_3670_cov_3.222407"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1613)
                     /locus_tag="PLU43_02610"
     CDS             complement(135..1613)
                     /locus_tag="PLU43_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO27329.1"
                     /translation="MNKRKIFGNLTIQTKLIFVFILTSMFIFAVNLYVYVNLNSMIGK
                     IDEIYASNVSLNEMTETLNSVQNSMTDYLKTKSTDALGLYYKSVQTYNRQLTDLNVIT
                     TNNDMKLMEKSIRNMSETYLNITNETVQAKRGRNVERYISGHEEATRLFSYINTYIYS
                     LNNEQFKSNSTNYETLLSSLKYSEVLCLSILVVVAFCNTLIIILVTRSITNPLKELSR
                     QADEIAQGRLDVELLEVKSADEVGVVTKGFNQMLLSIREYISQIRVRMEMESAMKEKE
                     LMMAAHLKDAQLKYLQAQTNPHFLFNTLNAGAQLAMMEGADKTNRYVQNMADFFRYNV
                     KKDKEDVTIAEEIELVDSYIYILNVRFSGDIHFRKEIDESLLQIRIPSMIIQPIVENS
                     VNYGIRNIEWEGLIILSLYREGDKVCISVKDNGVGISKERIEKILAQEIEPSNLSNDS
                     NGIGLANVIARLKLFFGREDIFSIKSDGENKGTETIVCIPIK"
     gene            complement(1606..2736)
                     /locus_tag="PLU43_02615"
     CDS             complement(1606..2736)
                     /locus_tag="PLU43_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO27330.1"
                     /translation="MEKKTENRFSRKEKILTGTLVSLTIVMLLTTLIGLIYFKQQIAG
                     SNLKDEEQYNEYERQYVLITKDIDDTFWDSVYEEMKEAGDETGVYVERLGRNLSMEYS
                     KNDLMEIAIDSHVDGIILEADESDETAALINKADAEGIPVVTIMRDAMGTTRKSFVGI
                     SNYNLGSEYGKLIVNASKELQERSAPEGNDTDQGQTLLPDPEAEADPVQVLILIDKNS
                     TDTSQNIIYTAIQEELEKEGFDKSKAQVDTAAISNDGAFSAEESIRDIFQNSEYQPDV
                     IVCLNELNTKSIYQTVVDQNKVGQIMIIGYYDSETILRAVQRKVIYATITVNTKQMGS
                     YCVKALNEYIDTGHVSDYFSVDFTLIDKDNVNEYLRSLDDNE"
     gene            complement(2839..>3670)
                     /locus_tag="PLU43_02620"
     CDS             complement(2839..>3670)
                     /locus_tag="PLU43_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27331.1"
                     /translation="KDVLLDDTVDLNEIALATAGAVGSDLANMINEAAINAVKNGRGS
                     VNQQDLLESIEVVMMGKEKKDRIMSKEEKKIVSYHEIGHALASALQKNTDPIQKITIV
                     PRTMGALGYAWYVPDEEKHLRSKTELEERIVTALAGRASEEIVFNSVTTGAANDIEQA
                     TGIARSMITQYGMSKNFGLVKLESVEEKYLNGRTVMECSDLTAAQIDKEIVKVLADAY
                     EEAKRLLKENRELMNELAEYLLQRESITGKEFMEIFRRAKGIAVPVKAETVEETVDEP
                     "
BASE COUNT          887 a          950 c          667 g         1166 t
ORIGIN      
        1 tgctgataaa ctgccctgct catttttcaa aaacttggtc gacagggctt aggtaggtgt
       61 tttgcgcttt ttgataaatg gggtaaatat atgtgctttt ctttttattt atcacttgac
      121 atcataccgc tggactattt tattggaata cagacaatcg tttccgttcc cttgttttca
      181 ccatcacttt ttatcgagaa aatatcttcc ctgccgaaaa acagcttcag tctcgcgatc
      241 acatttgcca gtccgattcc atttgaatcg tttgacagat tcgacggctc gatctcctgt
      301 gccagtattt tttcgatccg ttccttagaa atgccgactc cattatcttt tacactgata
      361 cagactttgt ccccttccct gtacagagac aggatgatca gaccctccca ttcaatattc
      421 cggatcccat agttaacact gttctcgacg atcggctgga tgatcatgct cggaatacgg
      481 atctgcagaa gagactcgtc gatttccttt ctaaaatgaa tatccccgga aaaccggaca
      541 tttaaaatat aaatatagct gtccaccagt tcgatctctt ctgcgatcgt aacatcttcc
      601 ttgtcttttt tgacattgta acgaaaaaaa tcagccatat tctgtacgta tctgttcgtt
      661 ttgtcggcac cctccatcat tgccagctgg gctcccgcat tcagcgtgtt aaacagaaaa
      721 tgcggattgg tctgtgcctg cagatatttg agctgggcat ccttcaaatg agctgccatc
      781 atcagctctt tttccttcat tgcactttcc atttccatcc gtacccggat ctgggaaata
      841 tactccctga tgctgagcag catctggtta aagcccttag ttacaacacc aacctcatct
      901 gcggacttta cttccaaaag ttcaacgtcc agacgtccct gcgcgatttc atctgcctgt
      961 ctggaaagtt ccttcagagg attcgtgatc gagcgtgtaa caagaataat gatcagcgta
     1021 ttacagaagg ccacaacaac aaggatcgac agacacaaaa cttccgaata tttcaaagaa
     1081 gacagcagcg tctcataatt cgtggaattg cttttgaact gctcgttatt caggctgtaa
     1141 atatacgtat taatatagga aaacagacgg gtcgcctctt catgaccgct gatataacgc
     1201 tccacattcc gtccgcgctt tgcctgcacg gtctcattcg taatattcag ataagtctcc
     1261 gacatattcc ggatactctt ttccatcagc ttcatatcat tgtttgttgt aatgacattg
     1321 agatccgtca gctgtctgtt gtacgtctgc acactcttat aataaagccc cagcgcgtcc
     1381 gtgctctttg ttttcaaata atccgtcatg ctgttctgta cgctgttcag tgtttccgtc
     1441 atttcattca gactgacatt gctcgcatag atctcgtcga tctttccgat catactgttc
     1501 aaatttacat aaacatacag gtttaccgca aaaataaaca ttgatgtcaa tataaagaca
     1561 aaaatcaatt ttgtctggat cgtaagatta ccgaatattt tccttttatt cattatcatc
     1621 caaactcctc agatattcgt ttacgttatc cttatcgatc aaagtaaaat ctactgaaaa
     1681 atagtcgctg acatgtccgg tgtcgatata ttcgtttaac gccttcacgc aatagctgcc
     1741 catctgcttt gtattgaccg tgatcgtcgc ataaatgact tttctttgaa cggcacgcag
     1801 gatcgtctcc gaatcataat acccgatgat catgatctgg ccgactttgt tctgatcaac
     1861 gaccgtctga taaatacttt ttgtattcag ctcatttaag cagacgatca catccggctg
     1921 atattcggaa ttttgaaaaa tatcacggat cgattcttcg gcactgaaag ctccgtcgtt
     1981 gctgatagcg gccgtatcca cctgcgcttt ggacttgtca aagccttctt tttcaagctc
     2041 ttcctgtatt gccgtataaa taatattctg tgacgtatcg gttgaatttt tatctatcag
     2101 gatcagcacc tgcacaggat ccgcttctgc ttccggatcc ggaagcaggg tctgtccctg
     2161 atccgtatca ttcccttccg gtgcggaacg ctcctgcagt tcttttgatg cattgacgat
     2221 cagctttcca tactcgctgc ccagattata attgctgatc ccgacaaagc tttttcttgt
     2281 agttcccatg gcgtcccgca tgatcgtaac taccgggatc ccttccgcat ctgccttgtt
     2341 gatcagagcc gccgtttcat cgctttcatc ggcttcaagg ataatcccgt ccacatggga
     2401 atcgatcgcg atctccatca gatcgttttt ggaatattcc atcgaaaggt ttctgccaag
     2461 acgctctaca taaacaccgg tctcatcacc agcctctttc atttcctcat aaacggaatc
     2521 ccagaatgta tcgtcaatat cttttgtgat cagtacatac tgccgctcat attcattata
     2581 ctgttcttca tccttcaaat tgcttccggc gatctgctgt ttgaaataga tcagtccgat
     2641 cagcgttgta agcagcataa cgatcgtcag ggatacaagg gttcccgtca atattttttc
     2701 ttttcttgaa aatcggtttt ccgtcttttt ttccattatg gccgaccttt gtaaaaatat
     2761 tctataaaag gcctgaacat aagctgttca agccttttta ttatagcgca gtttatatga
     2821 ttaataaagg aatttgtgct atggttcgtc tacagtctct tccactgttt cagctttgac
     2881 cggtacggca attccttttg ccctccggaa aatttccata aactccttac cggtaatact
     2941 ttctctctgc agcagatact ccgcaagctc attcatcagt tcacgattct ccttcagcag
     3001 gcgctttgct tcctcataag catccgcaag cactttcacg atctccttgt cgatctgtgc
     3061 cgcagtcagg tcactgcatt ccataacggt cctgccgttc agatattttt cttccaccga
     3121 ttccagcttt acaagaccaa aatttttcga catgccgtat tgcgtgatca ttgaacgtgc
     3181 gattccagtt gcctgctcga tatcattggc cgctcctgtc gtgaccgaat tgaaaacgat
     3241 ctcttccgac gcacgtcctg caagcgctgt cacgatccgt tcctccagtt ctgttttgga
     3301 acgcagatgc ttttcctcat ccggcacata ccatgcataa ccaagcgctc ccatcgtacg
     3361 cggcacgatc gtgatcttct ggatcggatc cgtatttttc tgcagtgcac tcgcaagcgc
     3421 atgcccgatc tcatgataag aaacgatctt tttctcttcc ttgctcatga tacgatcctt
     3481 cttttcctta cccatcataa caacttcgat cgattctaac agatcctgct gattgacgct
     3541 ccccctgcca ttcttgactg cgttgatagc tgcttcattt atcatatttg caaggtcaga
     3601 accgacagcc cccgctgttg caagcgcgat ttcattaagg tcgaccgtat cgtccaaaag
     3661 cacatccttc
//
LOCUS       DAOSYE010000101         3666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17811_length_3666_cov_3.050955, whole genome shotgun sequence.
ACCESSION   DAOSYE010000101 DAOSYE010000000
VERSION     DAOSYE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17811_length_3666_cov_3.050955"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLU43_02625"
     CDS             complement(<1..228)
                     /locus_tag="PLU43_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOO27332.1"
                     /translation="MKIIDGGVTAPNGFLAAGVEASIKYKNRKDMAMIYSQKPCVAAG
                     TFTTNVVKAAPVRWDQKIVADSAFAQAVVVNA"
     gene            complement(263..706)
                     /locus_tag="PLU43_02630"
     CDS             complement(263..706)
                     /locus_tag="PLU43_02630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO27333.1"
                     /translation="MIRVMKPEDYKEVYELWMCIKDFAIRSVDDAEEGIDLFLKRNPT
                     TSVVAVENGKLVGSILCGHDGRRGCMYHVCVHPDYRLRGIGKSMVVFAMEALRKEKIS
                     KVSLIAFTKNDIGNAFWNTIGWTKRNDLNYYDFVLNKENIINFNR"
     gene            complement(749..1789)
                     /gene="argC"
                     /locus_tag="PLU43_02635"
     CDS             complement(749..1789)
                     /gene="argC"
                     /locus_tag="PLU43_02635"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOO27334.1"
                     /translation="MIKAGIIGSTGYAGGELVRLLFNHKDVEIKWYGSRSYIDKKYYE
                     VFQNMFQIVADICKDDNLEKLAKEVDVIFTATPQGFLASVLTEEILSDTKVIDLSADF
                     RLKEVFVYEEWYRIKHQAPQFIREAVYGLCEINREDIRKARLVANPGCYTTCSILSIY
                     PLLKEGLIDKDSIIVDAKSGVSGAGRGAKTANLYCEVNESMKAYGVLTHRHTPEIEEQ
                     LGYAAGSGLKVIFTPHLAPMNRGILTTAYADLVKDVSYETVRAAYDKYYENEKYVRVL
                     DKDICPETKWVEGSNYMDVNFKIDNRTNRVVIMGALDNIVKGAAGQAVQNMNIMFGCR
                     ESEGLDLIPMYP"
     gene            2099..3322
                     /locus_tag="PLU43_02640"
     CDS             2099..3322
                     /locus_tag="PLU43_02640"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOO27335.1"
                     /translation="MKEKVILAYSGGLDTTAIIPWLKENYDYEVICVCIDCGQESELD
                     GLEERAQASGATKLYIEDVVDEFCDDYIVPCVQANAVYENKYLLGTSMARPVIAKRLV
                     EIARKENAAAICHGATGKGNDQIRFELGIKALAPDLKIIAAWRDDKWTMDSRESEIEY
                     CKAHNINLPFSADNSYSRDRNLWHISHEGLELEDPSNEPNYEHLLVLGVSPEMAPENG
                     EYITMTFEAGIPKSINGKAMKVSDIIRELNKLGGKHGIGIVDIVENRVVGMKSRGVYE
                     TPGGTILMEAHQQLEELILDRDTYALKKDMGSKLAQIVYEGKWFTPLREAVQAFIEST
                     QKYVTGEVKFKLYKGNIIKAGTTSPYSLYNESIASFKTGDLYDHHDATGFINLFGLSC
                     KVRAMKMAEAENNKK"
     gene            3516..>3666
                     /locus_tag="PLU43_02645"
     CDS             3516..>3666
                     /locus_tag="PLU43_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOO27336.1"
                     /translation="MNVILIIGSYLLGINLLGFFIMGIDKQRAKRQEWRIPETTLFSV
                     ALVGGS"
BASE COUNT         1017 a          882 c          666 g         1101 t
ORIGIN      
        1 cgcatttacg acaaccgcct gtgcaaacgc ggaatccgct actatctttt gatcccatct
       61 gaccggtgcg gcctttacga cattcgtcgt aaaggtgcct gcagcaacgc acggcttttg
      121 gctgtatatc attgccatgt ctttcctgtt tttgtacttg atcgatgctt ccactccggc
      181 cgccagaaat ccgtttggcg ccgtaactcc tccgtcgata attttcattg atttcactcc
      241 tcttattttc tccgtattcc ggttaacgat taaaatttat gatgttttct ttatttaata
      301 caaagtcata atagttcaga tcgtttctct ttgtccagcc gatcgtattc caaaatgcat
      361 ttccgatatc gtttttcgta aaagcgatca gcgatacttt actgattttt tcttttctga
      421 gagcttccat cgcaaaaacg accatgcttt ttccgatgcc tcttaaacgg taatccggat
      481 gaacacacac atgatacata catcctctgc gcccgtcatg accgcacaaa atactgccta
      541 ccagctttcc attttcgact gcaacaacac ttgttgtcgg atttcttttc aaaaataagt
      601 cgattccttc ttccgcatca tccacacttc tgatcgcaaa gtccttaatg cacatccaca
      661 gctcatatac ctctttataa tcctccggct tcattacacg tatcatatta ttcctccctg
      721 tattccatgc tgccgtcata agacttcttt atggatacat cggtatcaga tcaaggcctt
      781 ccgattccct gcacccaaac ataatattca tattctggac agcctgtcct gccgctcctt
      841 ttacaatatt gtcaagtgcc cccatgatca cgaccctgtt tgttctgtta tctatcttaa
      901 aattcacatc catataattg cttccttcga cccactttgt ttccggacat atatctttat
      961 ccaaaacccg cacatacttt tcattttcat aatacttgtc ataggcagct ctgactgttt
     1021 cgtaactgac atcttttaca agatctgcat aggccgtagt caaaatacct ctgttcatcg
     1081 gagccagatg cggcgtgaaa ataactttca gtccggaacc tgccgcatat ccaagctgtt
     1141 cctcgatctc cggcgtatgt ctgtgtgtca gaacaccgta ggccttcatg ctttcattga
     1201 cctcgcagta cagatttgcc gtctttgctc ctctgcccgc accggatacc cctgactttg
     1261 catcaacgat aatggaatct ttatcaataa gtccttcctt taataaagga tagatcgaaa
     1321 ggatcgagca ggtcgtatag cagcctggat tggcaacaag tcttgctttt ctgatatcct
     1381 ccctgttgat ctcacaaagt ccgtatactg cttcccgaat aaactgaggc gcctggtgct
     1441 ttatcctgta ccattcctca tagacaaaga cctcttttaa tctgaaatcc gcgcttaaat
     1501 cgataacctt cgtatcggat aaaatctcct ccgtcaggac cgacgccaaa aatccctgtg
     1561 gagtcgccgt gaaaatcaca tcaacttctt tggcaagctt ctctaagttg tcgtccttgc
     1621 aaatatctgc aacgatctga aacatattct gaaatacttc ataatatttc ttatctatat
     1681 agcttctcga gccataccat ttgatttcca catctttatg attaaaaaga agtctgacaa
     1741 gttcacctcc tgcatatcct gttgaaccga taattcctgc tttgatcata actgttcccc
     1801 tttcgccttt taaactgttc aaatactgcg attgcataat tatacatcta ttatgaatat
     1861 tgtgcaaccc attattttca gatcctatca ttttttattt cattttattc atgtttcttc
     1921 tatatttatg cactttttca tttttgggcg ctttctttct tcttttttgt attttatgca
     1981 taaattttaa tttttatgca taaaacactt gcattgccaa tacagttgtt gtatattagt
     2041 tacagtttac gaatcattat accataaatc aaacaatata ttcaggagga ttttaactat
     2101 gaaagaaaaa gtgatcttag cttactcggg tggacttgat acaacagcca tcattccctg
     2161 gttaaaagaa aattacgact atgaggtcat ttgtgtatgc atcgactgcg gacaggaatc
     2221 tgagcttgac ggccttgagg aacgcgcaca ggcaagcggt gccacaaagc tttatattga
     2281 agatgttgta gacgaattct gcgacgatta tatcgttccg tgcgtacagg caaacgcagt
     2341 ctacgaaaat aaatatctgc tcggtacttc catggcaaga cctgtaatcg ccaagcgtct
     2401 tgtagaaatt gcaagaaagg aaaacgcagc tgccatctgt cacggtgcca ccggtaaagg
     2461 aaacgaccag atccgttttg aactcggcat caaggcactt gctcccgatt taaagatcat
     2521 tgccgcatgg cgcgacgata aatggacaat ggattccaga gaatccgaaa tcgagtattg
     2581 taaagcccac aatatcaatc ttccgttttc agcagataac agctacagcc gtgaccgcaa
     2641 cctatggcac atcagccatg aaggcctgga actggaagat ccgtccaacg aacctaatta
     2701 tgaacatctt ctcgtccttg gtgtcagtcc ggaaatggcg ccggaaaacg gggaatatat
     2761 cacaatgaca tttgaagccg gcattccaaa aagcatcaac ggcaaggcaa tgaaagtctc
     2821 cgatattatt cgtgaactca acaagctcgg cggaaagcat ggcatcggca ttgtcgatat
     2881 cgttgaaaac cgcgtagtag gcatgaaatc gcgcggtgtc tacgaaacac ccggcggaac
     2941 gattctgatg gaagcacatc agcagcttga ggaactgatt ttggaccgtg atacatatgc
     3001 actgaaaaaa gatatgggtt ccaaactggc acagatcgtt tatgaaggca aatggttcac
     3061 accgcttcgc gaagcggtac aggcctttat tgaatccacg cagaaatatg tcacgggcga
     3121 agtaaaattc aagctgtata aaggcaacat catcaaagcc ggcacaactt ctccgtacag
     3181 cttatataat gaaagtattg cttcctttaa aacaggcgat ctttatgacc accatgacgc
     3241 caccggcttt atcaatctgt tcggtctttc ctgtaaagtc cgcgcaatga aaatggctga
     3301 agcagaaaat aataaaaaat aacagcagac cacctgtctg cgacaccata agcaggaccc
     3361 cgcatttaca aaacaagctg tatatacgag gtccttctta tgttttttcc tccttaaaga
     3421 cggtttaggc aattttacac tttatccgta ttttatttta tgctagtata aataagaaaa
     3481 gaaaaaccgt cttcacattt gcaagaggaa tttttatgaa tgtcatactg atcatcggaa
     3541 gttatttact cggtatcaac cttctgggat tttttataat gggaattgac aaacagaggg
     3601 ccaaacgtca ggaatggcgt attccggaaa ccacactgtt ctctgtcgcg cttgtaggcg
     3661 gaagca
//
LOCUS       DAOSYE010000102         3647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_17936_length_3647_cov_2.783686, whole genome shotgun sequence.
ACCESSION   DAOSYE010000102 DAOSYE010000000
VERSION     DAOSYE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17936_length_3647_cov_2.783686"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLU43_02650"
     CDS             complement(<1..1072)
                     /locus_tag="PLU43_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HOO27337.1"
                     /translation="MDIMELRKQLDEIDTQMAALFEERMAVCRDVAEYKIGHGKPVLD
                     KERENQKIASVGGLASNEFNARGLKELFGQIMAISRKLQYQMLQEKGMLKHANFIPVG
                     QLSEDRPRVVFQGTEGAYSQAAMEAYFGGETDHFHVDTFRDAMCAIEEGRADLAVLPI
                     ENSTVGIVDEIYDLLVEFENYIVGEQVIQIRNALIGLPGGTMEEIREVYSHPQALMQC
                     AGYLRKHTAWQQIGCRNTAVAARKILEEKDVTQAAIASPYAAKLYGLEIFEEGISDAA
                     DNSTRFIIVAGRKIYREDAQKVSVCFEVPHAAGSLYHILSHFIYNGLNMTKIESRPIR
                     DRNWEYRFFVDFEGNFSDMPVRN"
     gene            1257..2261
                     /locus_tag="PLU43_02655"
     CDS             1257..2261
                     /locus_tag="PLU43_02655"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOO27338.1"
                     /translation="MQKKLAIGIVIVLIILTGLVISSQFLFKIPENPEGTVGNTAGNL
                     NNSGLFREDGDTVFFSNPYDNDRLYSMNLDGSNKKLLLDVPVEYINSAGDYLYFYQKD
                     SDENVVFGLSGNMHGIYRLKKEGEQSPECLDRTLSGIVVLIDNAVYYQHYDNTEGMTL
                     YRTMTDDTKKEELSSEIINPACVIDGNIYYPDTENSFYLSVFNTESLESVPVVSERVY
                     NPVYEGGYIYYMNVPENYSLYRYSVSEGTDEQLTADRVDTFNVLGSYIFYQKNDAQDP
                     ALIRINTDGSNPEIVAKGNYTNINMTSYYTYFQGFGSTVPLYQTPTSGGIDVTEFSSE
                     "
     gene            complement(2274..3098)
                     /gene="folK"
                     /locus_tag="PLU43_02660"
     CDS             complement(2274..3098)
                     /gene="folK"
                     /locus_tag="PLU43_02660"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOO27339.1"
                     /translation="MDCIKIKNLEVFGRHGVSEEENRLGQKFSVSVQLYLDTRQAGLD
                     DDLQKSVHYGEVCHFITNFMKERTYQLIESAAEYLAQELLLNFKHVKRLDLEVKKPWA
                     PIGLPLEEVSVRIVRGWHTAYVALGSNVGECEEYLQSAVQSLEARTETSVERISKFME
                     TKPYGYVEQDDFLNGMVELKTLLTPEELLNVLHEIEEAAGRERTVRWGPRTLDLDIIL
                     YDDLVLDSEQLVIPHMDMQNRDFVLVPLMELAPNLRHPILNKTIAQLKNELESKLK"
     gene            complement(3085..>3647)
                     /gene="folP"
                     /locus_tag="PLU43_02665"
     CDS             complement(3085..>3647)
                     /gene="folP"
                     /locus_tag="PLU43_02665"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO27340.1"
                     /translation="IPVSIDTYKGNVAEAALAAGADLVNDIWGLKHDDKIAKVISKYG
                     VPCCLMHNRSHVEYVDFLADVKADLEQSLRLAKGAGIAADQIILDPGVGFAKSCEQNL
                     QIIKNMSYFKELGFPMLLGTSRKSVIGLTLALPVEERVEGTLVTTVLAVQAGYSFVRV
                     HDIKENVRAIQMLYALTDGRLDDGLH"
BASE COUNT          973 a          964 c          705 g         1005 t
ORIGIN      
        1 catttctgac cggcatatct gaaaaattgc cttcaaaatc cacaaaaaaa cgatattccc
       61 agtttctgtc cctgatcgga cggctttcga tctttgtcat attcaatccg ttataaataa
      121 aatgagataa aatatgataa agagaaccgg cagcatgcgg cacttcaaaa caaacactga
      181 ccttttgcgc atcctccctg tagatcttgc gtccggctac aataataaag cgcgtggaat
      241 tgtccgcagc atcactgatg ccttcttcga aaatttcaag tccgtagagc tttgccgcat
      301 aaggactcgc gatcgcagcc tgggtaacgt ctttctcctc cagtattttt ctggcggcca
      361 cggcagtatt gcggcagccg atctgctgcc atgcggtatg ctttctcaaa taaccggcac
      421 actgcatcag tgcctgcgga tgagaataaa cttccctgat ctcttccatt gttccgcccg
      481 gcagaccgat cagcgcattg cggatctgga tcacctgctc cccgacaata tagttttcaa
      541 actccacaag cagatcatag atctcatcta cgatcccgac cgtggaattt tctatgggaa
      601 gtaccgccag atctgctctt ccctcttcaa tggcacacat ggcgtcacgg aacgtatcta
      661 catggaaatg atcggtttct cctccaaaat aagcttccat ggcagcctgt gaataagccc
      721 cttctgttcc ctgaaatacg acacgcggtc tgtcttctga aagctgaccg accggaataa
      781 agttcgcatg ctttagcatc cctttttcct gcagcatctg atactgcagc ttacggctga
      841 tcgccatgat ctgaccaaac agctccttaa gaccgcgggc attgaattca ttcgatgcaa
      901 gtccgccgac agacgctatt ttctgatttt cccgttcttt atcaagcacc ggttttccat
      961 ggcctatttt atattcggca acatccctac agacagccat ccgttcttca aacaaagcag
     1021 ccatctgtgt atcgatctca tccagctgct ttctcaattc cataatatcc ataccgcttt
     1081 tgtgtccctc actttatcat tttattgttc aataccatta tatcaatata ttattatatc
     1141 gcacaacctc ttttcggcac aagtaaaagc aatgtaaaat aataccgcac ggcagaccgg
     1201 ataagattgc ataattttct gccatcgctt atactgaaag aaaggagtgg atacttatgc
     1261 aaaagaaact cgcgatcggc attgtcatcg ttttgatcat actgacaggt ttggtaatca
     1321 gttctcaatt cctttttaaa attcctgaaa atccggaagg aaccgtcgga aacacggctg
     1381 gtaatctgaa taattccggt ttgttcaggg aggacggcga tactgttttc ttcagcaacc
     1441 cctatgacaa tgaccgcctc tattccatga atctggacgg ctccaataaa aaacttttat
     1501 tggacgtgcc tgtcgaatat atcaactccg ccggtgatta tctttatttt taccaaaaag
     1561 attccgatga aaatgtcgta ttcggattaa gcggcaatat gcacggcatc taccgtctga
     1621 aaaaagaagg ggaacagtcc cccgaatgcc tggaccgcac gctttccggc atcgtcgtac
     1681 tgatcgataa cgccgtctat taccagcact atgataatac ggaaggcatg acgctgtacc
     1741 gtacgatgac ggacgataca aaaaaagaag agctcagcag cgaaatcatt aatcccgcat
     1801 gcgtcattga cggcaatatc tactatcctg atacggaaaa ttcgttttat ctgtcggtct
     1861 tcaacacgga atcgctcgaa tctgtcccgg ttgtttccga acgggtatac aaccctgttt
     1921 acgaaggcgg ctatatttac tacatgaatg taccggaaaa ttattccctg tatcgctaca
     1981 gcgtatcgga aggcactgat gaacagctga cagcagaccg tgttgatacg ttcaatgtac
     2041 tcggaagcta tattttctat cagaaaaacg atgcacagga tccggctctg atccggatca
     2101 acacagacgg ctccaatccg gagatcgtag ccaaaggaaa ctataccaat atcaatatga
     2161 cttcttatta tacatatttt cagggattcg gcagtacggt gccgctctat cagactccga
     2221 caagcggcgg aatcgacgtg acggaatttt cttccgaata accggttttg tcattatttt
     2281 aatttgcttt ccaattcatt ttttaactgg gcgatcgttt tattcaggat cggatgacgc
     2341 agattaggtg ccagctccat aagcggaacc agtacaaaat cacgattctg catatccata
     2401 tgcggaatca cgagctgttc cgaatcaagc accagatcat catacagaat aatatccaga
     2461 tcaagtgttc tcggtcccca gcgaacagta cgctccctcc ctgccgcttc ctcgatctca
     2521 tgcaggacat tcagcagttc ttccggtgtc agaagtgttt tcagctccac catcccattc
     2581 aggaaatcat cctgttccac atatccgtaa ggctttgtct ccataaattt tgagatccgt
     2641 tccacgctcg tttccgtgcg ggcttctaaa gactgcaccg cagactgcag atattcctca
     2701 cattctccaa cattagaacc caatgccaca taagccgtat gccagccccg cacgatcctg
     2761 accgatactt cctcaagcgg aagaccgatc ggcgcccatg gctttttaac ttccagatcc
     2821 agccgtttca catgcttaaa gttcaaaagc agctcctgtg caagatactc ggcagctgat
     2881 tcgatcagct gatacgtgcg ttccttcata aagttcgtaa taaaatgaca tacttccccg
     2941 taatggaccg acttttgcag gtcatcatcc agtccggcct gtctggtatc cagataaagc
     3001 tgtaccgata cggaaaattt ctgtccaagc ctgttttcct cttcactgac cccatgtctt
     3061 ccgaatactt ctaaattttt gattttaatg cagtccatca tccagcctcc cgtctgtaag
     3121 cgcatagagc atttgaattg ctctgacgtt ttcctttata tcatgaaccc ggacaaaaga
     3181 atatccggcc tgtaccgcca atactgtcgt gacaagcgtt ccctccacac gttcttccac
     3241 cggaagcgcc agtgtcagtc cgatcactga tttgcgtgag gttccaagca gcatgggaaa
     3301 acccagttcc ttaaaataac tcatgttttt tattatttgc aaattctgtt cacaggattt
     3361 tgcaaatccg acacccgggt cgagaatgat ctgatctgct gcaatccctg caccttttgc
     3421 aagacgaaga gactgttcca gatcggcttt cacatctgcc aaaaaatcca catattccac
     3481 atgagagcga ttgtgcatca gacagcaggg cactccgtat tttgaaataa ccttcgctat
     3541 tttatcgtca tgcttcagtc cccatatatc attgacaaga tccgctcctg ccgcaagcgc
     3601 agcctctgcc acattccctt tataggtatc tatcgacacc ggaatat
//
LOCUS       DAOSYE010000103         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18097_length_3626_cov_2.992439, whole genome shotgun sequence.
ACCESSION   DAOSYE010000103 DAOSYE010000000
VERSION     DAOSYE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18097_length_3626_cov_2.992439"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="PLU43_02670"
     CDS             <1..1648
                     /locus_tag="PLU43_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO27341.1"
                     /translation="DGEVLCRLSTGHSVSVKSEMEGSDGRKWYEISYSYNSVAGTGYI
                     CADYVTVEEAVQTTSSSAATGTIKGTSVRIRESAVNGTVLVQLDTGHAVTILGEITGS
                     DGYKWYQVQFTYQDSEKTGYVRSDLVNIITTVSQQTVSDADFEAVIADLPDSYKESLR
                     TLHTAYPNWQFEIVNTNLEWNDVVTAECSVGKNLVSSSSISSWKSTEPQAYNWISDSW
                     YGFDGGSWVSASKELIQYYLDPRNFLDESGVFQFETLEYQDYQNEAGVSNILSSSFMN
                     DSVTDADGTTRSYASMFTEAGSSAGISPYHLASRCLQEQGVYGTSQSISGTVSGYENL
                     FNFFNIGAYTANGNTATINGLIYAQGTDETYYRPWNTRYKSIMGSAKYVSEKYVAKGQ
                     NTLYFQKFNVVNSTNGIYSHQYMSNLLAASSEAARMKTAYSDLNTSLVFKIPYYNNMP
                     ETACTKPTSESNPNNYLAALWIDNQTFTPDFSSIVDTYYLTVDNSVESINIGATPVAS
                     TSSVGGVGQVSLNVGTNVISIVCKAQNGNTKTYTLTVQRN"
     gene            1701..>3626
                     /locus_tag="PLU43_02675"
     CDS             1701..>3626
                     /locus_tag="PLU43_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOO27342.1"
                     /translation="MYRKKWKAAGIIAMVSVFCILSANKVSAAGSVEITADEETAAVG
                     DTVTVTIEAEDPEDAAEAPEISVEYDTNRLSFLECDKDYGGGGGGLITLTDTTAEISF
                     SVLSGGTSEVTVSAILDGDGTDVPTDSVQIEVDGEDTAAAAEETEETETGVEEGTIMS
                     SDGTKVVSTVFADEFMPSLFHKTTATYEEQMVEAAQFDMGDILLLYVTDTSGNNGAFN
                     IYDQATGTLSDFLTINGIENRFIIVLSPDDTVTVPDGFTKATLQWNYQTLEAYMIADT
                     GDSDAETEGEASVSDDSVSSADFFLLYAISSEGNKGWYLYDQAEGTYQRYLQVVRTTV
                     DEDGNILSSLADSVSSEGSDQQANRRLLIIGIMAAVMLVMLIIMLNMLLKLKDFQSYD
                     YIDEEEDEDGEEERHERSSAIQRGTAWEESGMESGMNASMLADMQMNGEPSIQGDEEN
                     EDDGLEIIDDYENDEDFFKPKRKEQKEQKEKKPKKEKRHGKNKVKEEEPKAIDWSELE
                     HTIRESTDDERRPRGNRQEDLPPMYREKDEQPQPSQPLPEQKPQVQQPQPEQKPQPEQ
                     KPQPQPVRQPYRPQVQQPQPQPQPQPYQQPYQQQPQPYQHRTQPQPYRPQVQQPQPQS
                     YQQPRQQAYQQQSYGQQP"
BASE COUNT         1075 a          743 c          982 g          826 t
ORIGIN      
        1 agacggagag gtcctgtgca ggttaagcac aggacattct gtctccgtaa aatcggagat
       61 ggaaggcagt gacggaagaa aatggtatga aatatcctat tcctacaata gtgtcgccgg
      121 aaccggttat atttgtgcgg attatgtaac ggtagaggaa gctgttcaga caacgtcctc
      181 ctctgctgcg acaggtacga tcaaaggaac aagcgtccgt atcagggaga gtgctgtaaa
      241 cggaacagtt ctcgttcagc tggataccgg acacgcagtt acgatcctcg gtgaaattac
      301 aggaagcgac ggatataaat ggtatcaggt tcagtttacg tatcaggaca gtgaaaaaac
      361 aggttatgtc cgttccgatc ttgtgaatat tattacgacc gtatcgcagc agaccgtaag
      421 tgatgccgat tttgaggcag tgatagcaga tctgcccgac agctataagg aaagtcttag
      481 gacgctgcat acagcgtatc cgaactggca gtttgaaatt gtcaatacaa atctggaatg
      541 gaatgatgta gtgacagcag aatgctcagt cgggaaaaat cttgtttcca gttccagtat
      601 ttcttcgtgg aaatccacgg agcctcaggc ctataattgg atcagtgatt cctggtatgg
      661 ttttgacggc ggaagctggg tctccgcatc gaaagaattg atccagtatt atctggatcc
      721 gagaaatttt ttggatgaaa gcggagtttt ccaatttgag actttggaat atcaggatta
      781 tcagaatgag gccggtgtat ccaatatact gtcgtcgtct tttatgaacg attccgttac
      841 ggatgcggac ggtacgacaa gatcatatgc gtctatgttt acagaagccg gttcgtcggc
      901 cgggatcagt ccgtatcatc tggcatcccg ctgtctgcag gagcagggag tctatgggac
      961 gagccagagt atatcgggca cagtttccgg ttatgaaaat ttattcaatt tctttaatat
     1021 aggtgcgtat acggcgaatg gcaatacggc aacgataaac ggactgattt atgcacaggg
     1081 tacggatgag acttattaca ggccgtggaa tacccgctat aagtccatta tgggaagcgc
     1141 caaatatgta agtgaaaaat atgtggcaaa agggcagaat acgctgtatt tccagaaatt
     1201 taatgttgta aacagcacga acggcatata ttcccatcag tacatgagta atctgctggc
     1261 ggcatcgtcg gaagcagcca gaatgaaaac tgcctattcg gatttaaata catcccttgt
     1321 atttaaaatt ccttattata ataatatgcc ggaaacggcg tgcaccaagc caacttctga
     1381 gtcgaatccg aataattatc tggcggctct gtggatcgat aaccagacat ttacgcctga
     1441 tttcagttcg atcgtggata cttattatct gaccgtggat aacagcgtgg aatctatcaa
     1501 tatcggtgcc acaccggttg catccacatc gagtgtaggc ggtgtcggcc aggtgagtct
     1561 gaatgttggt acaaatgtaa tatcaattgt ctgcaaggcg caaaacggaa acacaaagac
     1621 gtatactctg acagtgcaga gaaattaaga aagattgtgc ataaaagctg ttttaaacag
     1681 cgtatgcgaa aggagtttgt atgtatcgga aaaaatggaa ggcggcagga atcattgcga
     1741 tggtttcggt gttttgtatt ttatctgcaa ataaagtatc ggcagcggga agcgtggaaa
     1801 taacagccga cgaagaaaca gcggccgtag gagacacggt gacggtaaca atagaggccg
     1861 aggatccgga agatgccgcc gaggctccgg agatatcggt agaatacgat acgaaccgct
     1921 tatcttttct ggaatgtgat aaggattacg gcggaggcgg aggcggtctg atcacgctga
     1981 cggacacaac ggccgaaatt tcattttccg tcctttccgg aggtacgtca gaagttacgg
     2041 tttccgcaat attggatggg gatggcacgg atgtgccgac cgacagtgtt cagatcgaag
     2101 tggatgggga ggataccgcg gccgcggcgg aagagaccga agagacggag accggtgtcg
     2161 aagaaggtac gatcatgtcg tccgatggta caaaggtcgt ttctactgtg tttgccgatg
     2221 agtttatgcc gtcgctgttc cataagacta cggcgactta tgaagagcag atggtagagg
     2281 ctgctcagtt tgatatgggt gatattttac tgctctatgt tacggataca tcaggaaata
     2341 acggagcttt taatatttat gaccaggcga caggtacttt gtcggatttt cttacgatca
     2401 atggaatcga aaaccgtttt attatcgtac tttcaccgga tgatacggta acggtgcctg
     2461 acggcttcac taaggcaacc ctgcagtgga attatcagac actggaagca tatatgatcg
     2521 cggatacggg cgattctgat gcggaaacag aaggagaagc gtcggtatcc gatgattccg
     2581 tatcaagtgc tgatttcttt ttattgtatg cgatcagctc cgaaggaaac aaggggtggt
     2641 atctgtatga ccaggcagag ggaacctatc agcgttatct gcaggtagtc cgcacaacag
     2701 ttgatgaaga cgggaatatt ttaagctcac tggcggattc ggtctcttcc gaaggaagtg
     2761 atcagcaggc gaacagaagg ctgctgatta taggtattat ggcagcggtc atgctggtca
     2821 tgctcatcat tatgctgaat atgcttttaa aactcaagga tttccagagt tatgattata
     2881 ttgatgaaga ggaagacgaa gacggcgaag aagaaagaca tgaacgaagc tctgctattc
     2941 agagaggaac tgcgtgggaa gagtccggaa tggaatcagg aatgaacgca agcatgcttg
     3001 cggacatgca gatgaacggt gagccgtcaa tacaaggcga tgaggagaat gaggacgacg
     3061 gattggaaat catcgatgat tatgaaaatg acgaggattt tttcaaacca aaacggaaag
     3121 aacagaaaga acagaaagaa aagaaaccta aaaaagaaaa acgccatggg aagaataaag
     3181 taaaagaaga ggaaccaaag gcaattgact ggtctgagct ggaacatacg atcagagaat
     3241 ctaccgacga tgaaaggagg ccgcgtggaa atcgtcagga ggatctgccg ccgatgtatc
     3301 gcgaaaaaga tgaacagccg cagccatcac agccgctacc ggaacagaag ccgcaagtac
     3361 aacagccaca gccggaacag aagccgcagc cggaacagaa gccgcagcca cagcccgtcc
     3421 gtcagcccta ccggccgcag gtacaacagc cgcagccgca gccgcaaccg caaccgtatc
     3481 agcaaccgta tcagcaacag ccgcaaccgt atcaacatcg gacgcagccg cagccctacc
     3541 ggccgcaggt acaacagccg cagccgcagt cgtatcagca gccacggcag caggcgtatc
     3601 agcagcagtc atatgggcag cagcca
//
LOCUS       DAOSYE010000104         3608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18225_length_3608_cov_2.925134, whole genome shotgun sequence.
ACCESSION   DAOSYE010000104 DAOSYE010000000
VERSION     DAOSYE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18225_length_3608_cov_2.925134"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLU43_02680"
     CDS             <1..1205
                     /locus_tag="PLU43_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOO27343.1"
                     /translation="GRKSETIPEDKKYFWGYERLDGRAGVRNELWVVPTTGCVNAVAK
                     AIAEGCLGLVGGSIEAVTAFAHPYGCSQMGEDQEHTRTILADLVHHPNAGGVLIVGLG
                     CENSGIGVLKEYIGKYDDTRVRFLQCQDCGDEVEEGICLLQELACAAGRLQRTKIRVE
                     KLVVGLKCGGSDGLSGITANPAVGQFSDILTELGGSTILTEVPEMFGAETILMDRADD
                     REVFEKTVRLINGFKEYFSSCHQPIYENPSPGNKEGGLSTLEDKAWGCTQKSGSSNVK
                     DVLAYGQLIEKAGLNLLSAPGNDLVASTALAASGAQIILFTTGRGTPFASIVPTIKLS
                     TNTELAVKKAHWIDFDCGRLLHTDITTEMLGRELYEYVLTVAGGMTVKSERAGYRDLA
                     LFKQGVTL"
     gene            1251..2672
                     /gene="uxaC"
                     /locus_tag="PLU43_02685"
     CDS             1251..2672
                     /gene="uxaC"
                     /locus_tag="PLU43_02685"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOO27344.1"
                     /translation="MKAFMDRDFLLRTETAKQLYESYADMQKIPVLDYHCHIDPREIF
                     EDRTYDTITQAWLGGDHYKWRQMRANGINENYITGDANDREKFQKWAETLPKLIGNPL
                     YHWSHLELRYYFGYQGNLNADTAQEVWELCNRKLCSPELSVRNLIRRSNVRLICTTDD
                     PIDSLEWHQKLKADQSFDVQVLPAWRPDKAMHIEKPEFTEYMETLSAVSGIEIKDLTS
                     LYEALKIRMDYFERNGCMVSDHALAYVMYQPADEKTADAVLKKRLQGEPVTERESLQF
                     MTAFMIFCAEQYYKRNWAMQLHYGCKRDNNGQMYRLLGSDTGFDCINNYAPSAQLADF
                     LNALSENGGIPKTIIYSLNPNDDAVISSVIGCFQSGEIKNKIQHGSAWWFNDHYTGMT
                     NQMKAFASVSVFSNFVGMLTDSRSFLSYIRHDYFRRILCDLIGGWVENGEYPYEETRL
                     GEIVKGISYNNAVDYFGFDLKKI"
     gene            2760..3446
                     /locus_tag="PLU43_02690"
     CDS             2760..3446
                     /locus_tag="PLU43_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOO27345.1"
                     /translation="MVNIGVYGDSILKGIQINPTNQKYCVNNHIDMETLSKKYAFQIK
                     NYSSFGCTVTKGKNMLKKGLAKNMECDAIVMDYGGNDCDYNWKNISEDPAGDYLPNTP
                     IELFQETYYDIIDTLKKRGILPILTTLPPLEPQKFFNWFCRDLNKDNILMWLGEIKHI
                     YDHQESYSKVVEKIARDTQVPLVDLRGAFLKSENVNTLLCEDGTHPNTSGQKVITSAF
                     NNFASGFLYA"
BASE COUNT         1086 a          660 c          937 g          925 t
ORIGIN      
        1 ccggcagaaa aagcgaaacc ataccggagg ataaaaaata tttttgggga tatgaacgct
       61 tagacggccg tgcaggagtc agaaatgaac tgtgggtcgt accgacgaca ggctgtgtca
      121 atgcggtcgc aaaagcgatt gcagaaggct gcctggggct tgtcggcggc agtattgaag
      181 cggtcacggc atttgcccat ccatacggtt gttcgcagat gggggaggac caggaacata
      241 cgagaacgat tctcgcagac cttgtccatc acccgaatgc cggcggagtt cttatagtag
      301 gacttggctg tgaaaattcc ggtattgggg tcttaaagga gtatatcggt aaatatgacg
      361 atacacgggt ccggtttctg caatgtcagg attgcgggga tgaagtggaa gaggggattt
      421 gtcttctgca ggagctggct tgtgctgcgg ggcggctgca gcggacaaag atcagagtcg
      481 aaaagcttgt tgtcggtctg aaatgcggcg gttccgacgg attgagcggg ataaccgcaa
      541 atccggcagt cgggcagttt tccgatattc tgacagagct tggaggaagt acgatattaa
      601 cggaagttcc ggaaatgttc ggcgcagaga cgattcttat ggatcgcgcc gatgaccggg
      661 aagtttttga aaagacagtg cggcttatta acggattcaa agagtatttt tcctcctgtc
      721 atcagccgat ctatgagaat ccgtctcctg gaaataaaga aggcggactt tccacattag
      781 aagataaagc atgggggtgc acacagaagt ccggcagttc aaacgtgaag gatgtgcttg
      841 catacggaca gctgatagaa aaggcgggcc tgaatctatt gtcggcaccg ggcaatgatc
      901 tggtggcaag cactgcactt gcagccagcg gtgcgcagat cattttattt acgacaggcc
      961 gtggaacgcc gtttgcaagc attgttccga cgatcaagct ttcaacaaat acggaactgg
     1021 cagtaaaaaa ggcacattgg atcgactttg actgcggacg tctgcttcat acggatataa
     1081 cgactgaaat gcttggaaga gagctgtatg aatatgtact gacggtggca ggcggtatga
     1141 cggtaaaaag tgagagagca ggctacagag accttgcctt gtttaaacag ggcgtaacat
     1201 tgtaaaagcg gtcagattct atatgatcgg cagacgggag gacggcagat atgaaagcat
     1261 ttatggatcg ggattttttg ctgcgtacgg aaacggcaaa acagctatat gaaagctatg
     1321 cggatatgca gaagattccg gtattggatt atcactgtca cattgacccg cgtgaaattt
     1381 ttgaagacag aacttatgat acgatcacgc aggcgtggct tggcggtgat cattataaat
     1441 ggcgccaaat gcgggctaac ggaattaatg agaactatat tacaggggat gccaatgacc
     1501 gggagaagtt tcaaaaatgg gcggaaacgc tgccgaagct tatcggtaac ccgctgtacc
     1561 actggtctca tctggaactg cggtattatt ttggatacca gggaaatctg aatgcagata
     1621 cggcacagga agtatgggag ctgtgtaaca ggaagctctg cagtccggag ctttccgtcc
     1681 gcaatctgat cagaaggtcg aatgtgcgcc tgatctgtac gaccgatgat ccgatcgatt
     1741 ccctggaatg gcatcagaag ctgaaagcag atcaaagctt tgacgtccag gtccttccgg
     1801 catggcggcc ggataaggca atgcatattg agaagccgga atttaccgaa tatatggaaa
     1861 cgctttcggc agtatccggt atcgagatca aagatctgac atcattgtat gaagccctga
     1921 aaattcggat ggattatttt gaaaggaacg gatgcatggt gagtgaccat gcgcttgcct
     1981 atgttatgta tcagccggca gatgaaaaga ctgcggatgc agttctgaaa aagaggctgc
     2041 agggtgaacc tgttacagaa agggaaagtc ttcagttcat gacggcattt atgatctttt
     2101 gtgcagaaca gtattataaa agaaactggg caatgcagct gcattatggt tgcaaacgtg
     2161 ataataacgg gcagatgtat cggctccttg gatcggatac cggatttgac tgtataaaca
     2221 attatgcgcc gtccgcacag cttgctgatt ttctgaatgc gctgtctgaa aacggcggaa
     2281 ttccaaaaac cattatctat tcactcaatc ccaatgacga tgcggttatt tcttcggtta
     2341 tcggctgttt ccagagcgga gagataaaaa ataagataca gcatggttcg gcctggtggt
     2401 ttaatgatca ctataccgga atgaccaacc agatgaaagc attcgcaagt gtgtcggttt
     2461 tcagcaattt tgttgggatg ctgactgatt cccgctcttt tctgtcatat atcagacatg
     2521 attattttcg caggatcctc tgcgatctga tcggcggctg ggtcgaaaac ggagagtatc
     2581 cgtatgaaga aacccggctt ggtgaaatcg ttaagggaat ttcgtataat aatgctgtgg
     2641 attattttgg tttcgatctg aaaaaaatct gattgacgat tgacaaacga atatgaacgt
     2701 tctatcatgt agttataaat actacataat agagcaacaa cagattcgag gtgaatagta
     2761 tggtcaatat aggagtatat ggagattcga tcctgaaagg aattcagatc aatccgacaa
     2821 accaaaaata ctgcgttaat aatcatattg atatggaaac attgagtaaa aaatatgcgt
     2881 tccagatcaa aaattattcc agctttggat gtacggtaac aaaaggaaag aatatgctga
     2941 aaaaaggtct ggcgaaaaat atggaatgtg atgcgatcgt gatggactat ggcggaaacg
     3001 actgcgatta taactggaag aacatttcgg aagatccggc aggagattat ttaccgaata
     3061 caccaataga gctctttcag gaaacctatt atgatatcat cgacacttta aagaaaagag
     3121 gaattctgcc gatactgacg acgctgcccc cgctggaacc gcaaaaattt tttaactggt
     3181 tctgcaggga tctgaataaa gataatatac tgatgtggct tggtgagatc aagcatattt
     3241 atgatcatca ggaaagttat tctaaagtcg ttgaaaagat cgcgcgggat acacaagtgc
     3301 cgttagttga cttaagagga gcgtttttaa aatcagagaa tgtcaatacg ctgctttgcg
     3361 aggacggcac gcatccgaac acatcaggtc agaaagtcat aacatcggcg tttaacaatt
     3421 ttgcaagtgg atttttgtat gcttaggaga gttatttttt tgattgactt tttattttat
     3481 atgaagtaat attataaaca actgcaataa acagtataaa attaatttga cataaatctt
     3541 aatatacact agtaaattat aagtgagaag taaatatata ttaattacat taattaaata
     3601 taaatatt
//
LOCUS       DAOSYE010000105         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18301_length_3598_cov_3.391194, whole genome shotgun sequence.
ACCESSION   DAOSYE010000105 DAOSYE010000000
VERSION     DAOSYE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18301_length_3598_cov_3.391194"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLU43_02695"
     CDS             complement(<1..465)
                     /locus_tag="PLU43_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27346.1"
                     /translation="MASLSLKNVCKVYPNGFEAVKDFNLEITDQEFIIFVGPSGCGKS
                     TTLRMIAGLEEITSGELKIADRVVNDVEPKDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKVPKDEIDKMVKEAAKILDLTPLLDRKPKALSGGQRQRVAMGRAIVRNPKV"
     gene            complement(689..2179)
                     /locus_tag="PLU43_02700"
     CDS             complement(689..2179)
                     /locus_tag="PLU43_02700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27347.1"
                     /translation="MNKQKKDNTNIIQWAIPALLLVLFLIITLLNYSATMRNDAKEKV
                     MSKLESQAESLCEYYEDTLTSLTNIAEITAEYCSISSDVFQTNNIALLSDIVKQTGVK
                     KAYIQKLDNSAVDESGNTYVKISNKSEYLDILSDSSQVTKVIQDEDGENVLLVTAPIK
                     NSSEILGKLILVYKPDKLEEVVSRPDYLLKNTYALVSDEGKVIECVGKETGLFSIGDN
                     LFTKFEEVSFLDGSLKYLTQNISNQKSGEAKIRKNEEIEKYVVYAPVGEYHSSIVIFL
                     NHDQVEKTISEECKSTRTLMYKIGSSFVVFILIILMINIVSRTKYINENRELQSKAET
                     DLLTDLLNKVATEKKIKEYLQNEGKGKTSIMFVLDIDNFKKINDTMGHAFGDEVLRTL
                     GKQIRAEFRVDDIIGRTGGDEFTILLKDLNDDATMKREAKRVEQFFKNFKVGEYTKYF
                     ATASIGAAIYPKDASDFESLYKASDQALYKAKKRGKNQLAFYSDAE"
     gene            complement(2230..2907)
                     /locus_tag="PLU43_02705"
     CDS             complement(2230..2907)
                     /locus_tag="PLU43_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HOO27348.1"
                     /translation="MNRIFDMEGPVFTTLNRLADLVWLNILYIICCIPIFTIGASTTA
                     MYYVTMKMAKNEEGYITRSFFKSFKLNFKQATLLWLLALAAGGILLLDYAIMGGRFGD
                     IVEMNEILRKVVTGILFAVGGLYVFVMIYLFPLLAKFDNSIKNTIKNALLISIRHFPF
                     TLLLIVIPVVPFVLMYFVNQLVLFVFFLVFSLVAFASSYIYVKIFANYLPGEEKTEDE
                     TGEREDA"
     gene            3259..>3598
                     /locus_tag="PLU43_02710"
     CDS             3259..>3598
                     /locus_tag="PLU43_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO27349.1"
                     /translation="MEFNEKLQQLRKQRNLTQEELAETLYVSRTAVSKWESGRGYPSI
                     DSLKAISRLFSVSIDELLSSEELISLAETESKEKAESFSDMVFGILDCTTMLLLCVMP
                     TAIMQFNGLIK"
BASE COUNT         1059 a          842 c          547 g         1150 t
ORIGIN      
        1 gactttcgga ttacgaacaa ttgcacggcc cattgcaaca cgctgtctct gtccacctga
       61 caaagccttt ggctttctgt caaggagcgg tgttaaatca aggatcttgg ctgcttcctt
      121 taccatcttg tcaatttcgt ccttcggtac ttttctaagc ttcagaccaa acgccatatt
      181 gtcatatacc gacatgtgcg gatacaacgc atagttctgg aataccatcg cgatatccct
      241 gtccttaggc tccacatcat ttacaactct gtctgcgatc tttaattccc ctgaagtaat
      301 ttcctcaagt cctgcgatca tacgaagtgt tgttgattta ccacagcctg aaggtcctac
      361 aaaaataata aattcctgat ccgtaatctc aagattgaag tctttgactg cttcaaatcc
      421 gtttggatat actttacaaa catttttcaa agataaactt gccatttctc aattcctcct
      481 aaaaataatt gtgctgcaag gtgtactgta caccttattc ctcatttact gatagtatat
      541 caaaagaggt ttttcttgaa catgccacta ttccacaaag ttttttctat atcctataca
      601 tgttgcctat acccataatg gcactttgcg caaacttttc agctttttca ttcaaaatat
      661 atccatcctg atggaagggt tcctctgcct attctgcatc gctgtaaaaa gcaagctgat
      721 ttttccctct cttcttcgcc ttgtaaagcg cctgatccga tgctttataa aggctctcaa
      781 aatccgaggc atcttttgga taaatagcgg ccccaatact tgccgtggca aaatatttcg
      841 tatactctcc caccttaaag tttttaaaaa actgctccac ccttttcgct tccctcttca
      901 ttgttgcatc atcgtttaaa tctttcaata agatcgtaaa ttcatctccg ccggttcttc
      961 cgataatatc atccacacga aactccgcac ggatctgttt gccaagcgtt cttaatactt
     1021 catcaccaaa cgcatgtccc attgtgtcat tgatcttttt aaaattatca atatccagaa
     1081 cgaacatgat cgacgtcttt ccttttcctt cattctgcag atattccttg atcttcttct
     1141 cggtagccac tttattaagc agatccgtaa gcaggtctgt ttcagccttg ctctgcagct
     1201 ccctgttttc attaatatac tttgttctgc tgacaatatt gatcattaaa ataattaata
     1261 taaacacaac aaaagaactg ccgatcttat acatcagcgt tcttgtactt ttacattctt
     1321 cagaaattgt tttttccacc tgatcgtgat tcaaaaaaat aacaatggat gaatgatatt
     1381 cccctaccgg tgcataaact acatattttt ctatctcttc attctttctg atcttagctt
     1441 cgccgctttt ctgattgcta atattctgcg ttaaatattt cagtgaaccg tccagaaaac
     1501 taacctcttc aaacttggta aacaaattat ccccaattga aaaaagaccg gtttctttcc
     1561 caacgcactc gatcaccttg ccttcgtcag acacaagcgc atatgtattc tttaacagat
     1621 aatccggtct ggatacgacc tcttccagtt tatccggctt ataaaccaat ataagctttc
     1681 ccagtatttc cgaagagttt ttgattggcg cagtaacaag gagtacattt tctccgtctt
     1741 catcctgaat aactttcgtg acctgtgaac tgtcgctcag aatatccaga tattcacttt
     1801 tattgctgat cttcacatag gtgtttccgc tttcatcaac tgcggaatta tccagtttct
     1861 gaatatatgc cttttttaca cctgtttgtt tcacaatgtc cgagagcagc gcaatattgt
     1921 ttgtttgaaa tacatccgac gaaatcgaac agtattccgc cgtaatttcc gcaatattcg
     1981 tcagagatgt cagcgtatcc tcataatatt cgcacagaga ctccgcctga ctctctaact
     2041 tgctcattac tttttccttt gcgtcatttc tcattgtcgc gctgtaattc agtaaagtaa
     2101 taataagaaa aagcaccaga agcaatgctg gtatcgccca ctgtataata tttgtattat
     2161 cctttttctg tttattcatg cataaacacc tataccctca tatttctgat tattccctat
     2221 tctttatttt tatgcatcct ctctttctcc tgtctcgtcc tccgtctttt cctctcccgg
     2281 aagataattt gcaaaaatct ttacataaat ataagaagaa gcaaatgcaa ccagagaaaa
     2341 aacaagaaaa aacacaaaca gcaccagctg attcacaaaa tacattaaaa cgaacggaac
     2401 aacaggaatg acgatcagta ataatgtaaa tggaaaatgt ctgatactga tcagtaacgc
     2461 attctttatc gtattcttta tgctgttatc aaatttggca agcagcggaa acaaatagat
     2521 cataacaaat acatacaggc ctccgacagc aaacaggatt cctgtaacaa cttttctgag
     2581 gatctcattc atctctacaa tgtctccgaa tcggcctccc atgattgcat aatccagcag
     2641 aagaatccct cccgccgcca gtgcaagcag ccaaagtaat gttgcctgtt taaaatttaa
     2701 cttaaatgac ttaaaaaaac ttcttgtaat ataaccttct tcattcttgg ccattttcat
     2761 cgttacataa tacatcgccg ttgtagacgc tccgatcgta aaaatcggta tacagcaaat
     2821 aatatacaaa atattcagcc atacaagatc cgccagtcta tttaacgttg taaatacagg
     2881 cccttccata tcaaaaattc tgttcatcgc aattactcct ttaatttcat tatttaatta
     2941 cctgtacaac aaccatttat tgtcagttat tagtttagtt tcttaatatt attttttatt
     3001 atagcctaac aatttgaaaa ttgcaataaa ataccccccg tatcagccgg tttgagccat
     3061 ttatttaaac aatttttaca gccccctgct attgttatga aggtggttcc catgaaaata
     3121 acactgaaaa gctgcggctg ctattgatca taattcagtg gaaagatcta tgccggcatg
     3181 atatagttaa atagcaatct ttcatatata atcttgttcg gaaaaaccgt aaacacagca
     3241 ggagtgaggc acagagcaat ggaattcaat gaaaaattac agcaattacg aaaacagaga
     3301 aatcttacac aggaagaact tgccgaaacg ctatatgtat ctcgcacggc tgtttccaaa
     3361 tgggaatcgg ggcgaggata ccccagtatt gactccctca aagcaatttc aagactcttc
     3421 tcagtatcaa ttgacgaact gctttccagc gaagaactga tatcccttgc cgaaacggaa
     3481 agcaaagaaa aagcagaaag cttctccgat atggttttcg gaatacttga ctgcacaaca
     3541 atgctccttc tatgtgtcat gcccacggcg atcatgcagt tcaacggtct gatcaagg
//
LOCUS       DAOSYE010000106         3580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18448_length_3580_cov_3.358582, whole genome shotgun sequence.
ACCESSION   DAOSYE010000106 DAOSYE010000000
VERSION     DAOSYE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18448_length_3580_cov_3.358582"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLU43_02715"
     CDS             complement(<1..517)
                     /locus_tag="PLU43_02715"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO27350.1"
                     /translation="MKKLTKIKKKGSKSLYGSFLYMTLLPLFLFGIVTIVFSSKALSS
                     SIQTEVSNNLKNVSRSVLIAYDRMYPGDYNIVIDGDKVLFYKGIHLLSEDYTLIDNIR
                     EQTGVEVSLFFYDTRLLTTISNREERFVNTGANPQIVDEVIKKKSAKFYNNVNINGVP
                     YFAYYEPVFSQD"
     gene            complement(754..3039)
                     /locus_tag="PLU43_02720"
     CDS             complement(754..3039)
                     /locus_tag="PLU43_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOO27351.1"
                     /translation="MDIKKIIREMTLEEKAGLCSGADFWHTKAVERLGLPDIMVSDGP
                     HGLRKQDMEGDHLGMNDSIKAVCFPAACATAASFDRELITTLGEALGDECQAEDVSVI
                     LGPAVNIKRSPLCGRNFEYFSEDPYLAAEIATGHIRGVQSKQVGTSIKHFLANNQEHR
                     RMTSNSEADERTLREIYMPAFEGAVKNAKPWTVMCSYNKINGEYASESYHYLTEVLRD
                     EWGFDGFVMSDWGAVNNRVEGVKAGLDLEMPSSGGINDALLVQAVKDKQLDEKVLDLA
                     AERILTIVDRYKKNRRPDTVWDKEAHHVLARKIESECAVLLKNEGILPLDPSDVTAFI
                     GYFADCPRFQGGGSSHINSTKTESALEAAKDKNVVYAQGYEIDTDRANEELIKKAVDT
                     AKKAKTAVIFAGLPDDFESEGYDRSHMAMPECQNALIEAVAAVQPNTVVVLHNGSPVE
                     MPWIGRVKGVLEAYLGGQAVGGAVVDILYGIVNPSGKLAETFPIKLEDNPSFLYYGGE
                     KDVTQYKEGIFVGYRYYDKKKMDVLFPFGHGLSYTTFAYGNMKTDRQVLKDTETLTVS
                     LDVTNTGKVAGKEAVQIYVEALDSEAIRPVRELREFAKVELKPGETKTVSFVLGKRAF
                     SYYNVQIKDWYAEPGIYEIHAGTSSRELLASAKIEVQSAVKLPVTYHLNSIIGDIQAD
                     PDVAEIIRPLIDKFNAVSPISASAEGTSEAAEAAISSQMEEAMFRYMPVRQILSFAPG
                     VSTLEELQAVLDQMNKKKKGV"
     gene            complement(3064..>3580)
                     /locus_tag="PLU43_02725"
     CDS             complement(3064..>3580)
                     /locus_tag="PLU43_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOO27352.1"
                     /translation="QDTKPNAQPYQVKKSIRLSVANESMQDEKLTVNWELRKNTGEIL
                     LAGEEYAEVPKLSSVWLDKTELLQADLYEDYVSYRLSRGKEILSFGTVIFCAPKHYRF
                     LDPKLSVLEEDGELIVKSEAYARSVEIRNTNDDLILDDNFFDMDPGERRVKVLKGTTD
                     GLKIRSVYQIR"
BASE COUNT          888 a          976 c          681 g         1035 t
ORIGIN      
        1 cgtcctgaga aaaaaccggc tcataatatg caaaatacgg aaccccgttg atattgacat
       61 tattatagaa ttttgcggat ttctttttga tgacctcatc cacgatctgc ggattggccc
      121 ccgtattgac aaaacgctct tccctgttgg aaattgttgt caaaagacgc gtgtcataaa
      181 aaaacagcga gacttccaca cctgtctgtt cccttatatt atctattaaa gtataatcct
      241 cactcagcag atggatcccc ttataaaaca gaaccttgtc cccgtcgatc acaatattat
      301 aatcgcccgg atacattctg tcatacgcga tcaaaacaga cctgctgaca tttttcagat
      361 tattgctgac ttcagtctgg atagaagaag aaagtgcttt ggagctgaaa acgatcgtaa
      421 cgatcccaaa cagaaacagg ggcagcaatg tcatatataa aaaagaacca tagagactct
      481 ttgatccttt tttcttgatt tttgtaagtt ttttcatatt tcggctgtcc tctccggcaa
      541 aaaatattgt tatactatct atttcggctc ataaaaccat attctttact ttatttcatg
      601 aagataccct gctgctccca catatccggt ctgaaacgtc cggacggctg tcattcgtag
      661 tatatcaata atcatgcaaa aaagcaatga cctctccctt cgtacaaagc aaaagggaaa
      721 ggccattatt tcagaaatcc cgtttattaa aactcagact ccttttttct ttttattcat
      781 ctggtcaagc actgcctgca attcctcaag cgtactgact cccggtgcaa agctgagtat
      841 ttggcgaaca ggcatataac gaaacattgc ctcctccatc tgtgaagata tcgctgcttc
      901 tgctgcctcg gatgttcctt ccgcggatgc gctgatcgga ctgaccgcat taaatttgtc
      961 gatcagaggc cgtatgatct cagccacatc aggatctgcc tgaatatctc cgataatgct
     1021 gttcaggtga tatgtaaccg gcagcttcac cgcagactgc acttcgatct tagccgatgc
     1081 aagaagctcc cgtgaagaag tgcctgcatg gatctcatag ataccgggtt ccgcatacca
     1141 gtctttgatc tgaacattgt aataggaaaa tgcccgtttg ccaagcacaa agctgacagt
     1201 ttttgtttcc cctggtttca gctccacctt tgcaaactca cgcagctccc tgaccgggcg
     1261 gatagcctca ctgtccaaag cttccacata gatctggacc gcttcctttc ccgcaacctt
     1321 tccggtattt gtgacatcaa gggaaactgt taatgtttcc gtatctttca gcacctgcct
     1381 gtccgttttc atattcccat acgcaaacgt cgtataactc agtccgtgtc caaacggaaa
     1441 cagaacatcc attttctttt tatcataata acggtagccg acaaatatgc cttctttgta
     1501 ctgtgtcaca tctttttcac cgccgtaata gaggaatgac ggattgtctt ccagcttgat
     1561 cggaaatgtt tcggcaagct ttccgctcgg attgacgatg ccgtataaaa tatcaacaac
     1621 ggctcctccg acggcctgac ctcccagata tgcctccaat acgcctttta ccctgccgat
     1681 ccacggcatt tcaacaggcg aaccgttatg cagtacgaca acggtattag gctgtacggc
     1741 tgccactgct tcgatcagcg cattctggca ttccggcatt gccatatgag aacggtcata
     1801 gccctccgat tcaaaatcat cgggaagtcc ggcaaaaata accgctgtct ttgctttttt
     1861 agccgtatct accgcttttt tgatcagctc ctcatttgcc cgatccgtat cgatctcata
     1921 cccctgtgca tagacgacat ttttatcctt tgcggcttca agcgctgact ccgtctttgt
     1981 cgaattgata tgtgaacttc ccccgccctg aaatctcgga cagtctgcaa aatagccgat
     2041 aaacgccgtt acatcggaag gatccagcgg aaggatccct tcattcttta aaagcactgc
     2101 acattcgctt tctattttcc ttgcaagcac atgatgcgcc tctttatccc aaaccgtatc
     2161 cgggcgcctg ttttttttgt aacggtcaac gatcgttaat atccgctcag ctgccaggtc
     2221 caaaaccttc tcatccagct gtttgtcctt taccgcctgc acaagcaggg catcgttgat
     2281 cccgccggaa gacggcattt ccagatcaag tccggccttt acaccttcca ccctgttgtt
     2341 gaccgctccc cagtcactca ttacaaatcc gtcgaatccc cattcatccc gcaaaacttc
     2401 cgtaagataa tgataactct cggatgcata ttctccgttg atcttattat aggaacacat
     2461 cacagtccat ggctttgcat ttttaacggc tccctcaaac gccggcatat aaatttcccg
     2521 caatgttctc tcgtctgctt cggaatttga cgtcatacgc ctgtgttcct ggttgtttgc
     2581 cagaaaatgt ttgatacttg ttcccacctg cttactctgc acacccctga tatgtccggt
     2641 cgctatttca gcggcaaggt aaggatcctc cgaaaagtat tcgaaatttc tgccgcacag
     2701 gggggagcgt ttaatattga ctgccggccc taatataaca cttacatcct ctgcctggca
     2761 ctcgtctcca agcgcttccc caagcgttgt tataagctct ctgtcaaaag aagctgcagt
     2821 tgcacaggca gccggaaagc agacagcctt aatgctgtcg ttcatcccca gatgatcccc
     2881 ttccatatcc tgtttcctga gtccgtgagg tccgtcgctt accataatgt ccggcaggcc
     2941 cagacgctcg accgcctttg tatgccaaaa gtcggcaccg gaacaaagtc cggccttttc
     3001 ttccagggtc atctccctga ttattttttt aatatccatg atctactcct tcttttaaaa
     3061 aagttaacgg atctgataga cacttcttat tttcagtccg tcagttgtac ctttcaaaac
     3121 ttttacccgt ctttcccctg gatccatatc aaaaaaatta tcgtctaaaa tcagatcgtc
     3181 gttcgtattt cggatctcga ccgaccgcgc ataagcctcc gacttaacga tcagctcccc
     3241 gtcctcttcc aaaacagaca gctttggatc caaaaagcga tagtgcttcg gcgcacagaa
     3301 aataacggtt ccgaacgaca ggatctcttt gccgcggctt aaacgatagc ttacataatc
     3361 ttcatacaga tctgcctgca gcagttccgt tttatcaagc catacggaag aaagctttgg
     3421 cacttctgcg tattcttccc ctgcaagcag gatttccccc gtgtttttcc gaagctccca
     3481 gttgaccgtc agcttttcat cctgcatgct ttcatttgca acggaaagcc ggatggattt
     3541 ctttacctga tagggctggg cattcggctt tgtgtcctgt
//
LOCUS       DAOSYE010000107         3559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18606_length_3559_cov_2.516553, whole genome shotgun sequence.
ACCESSION   DAOSYE010000107 DAOSYE010000000
VERSION     DAOSYE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18606_length_3559_cov_2.516553"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLU43_02730"
     CDS             complement(<1..172)
                     /locus_tag="PLU43_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOO27353.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFNAEGYEVVAINDLTSPTMLAHL
                     LKYDSSQGKYALA"
     gene            407..1216
                     /locus_tag="PLU43_02735"
     CDS             407..1216
                     /locus_tag="PLU43_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOO27354.1"
                     /translation="MSSDFHLHTSFSSDSETPMSSMIEQGIRTGLSYLCFTEHMDKDY
                     LKRTDDDPDFEVDTDAYLTKYRELKEKYKDRITILFGVELGLQPHVADYYRQYTADYP
                     FDFIIGSSHLCHRKDPYFPEFFEGREEHLAYREYFESILENIQTYDGYDIYGHLDYVV
                     RYGPYKNKFYSYQRYADIIDEILRALTASSKGIEINTGGFKYGLGHPNPTEEIIKRYR
                     ELGGEIITIGSDAHEPNYIAYDFDKIPSILKNAGFQYYTIFKERKPAFIKL"
     gene            complement(1222..2298)
                     /gene="mnmA"
                     /locus_tag="PLU43_02740"
     CDS             complement(1222..2298)
                     /gene="mnmA"
                     /locus_tag="PLU43_02740"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOO27355.1"
                     /translation="MKKKVAVGMSGGVDSSVAAYFLKEQGYEVIGATMRIWQDEEEMQ
                     KEENGGCCGLSAVDDARQVAGLLGIPYYVMNFRKEFKEQVIDYFTEEYLRGRTPNPCI
                     ACNRYVKWDSLLKRSLEIGADYIATGHYAQVSRLANGRFTLKAAPSVKDQTYALYNLT
                     QEQLSHTLMPVGAYTKGEIRAVAEKIGIAVANKPDSQDICFIPDNDYGRFICESAPDR
                     VHGEGNFVLEDGTVVGKHKGVVYYTIGQRKGLQLAMGHPVFVREIRPETNEVVIGENE
                     DIYKTELICDKINAMAVPEIEDKKRLRAKIRYSHQGVSCTLHRLDADRIRVVFDEPVR
                     AVTPGQAVVFYEEDYIYGGGTIVC"
     gene            complement(2347..2787)
                     /gene="nifU"
                     /locus_tag="PLU43_02745"
     CDS             complement(2347..2787)
                     /gene="nifU"
                     /locus_tag="PLU43_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOO27356.1"
                     /translation="MYSEKVMDHFSNPRNVGEIEDASGVGTVGNAKCGDIMRIYLDID
                     DDQIIRDVKFKTFGCGAAVATSSMATELVKGKSVKDAMQITNKAVMEALDGLPAVKVH
                     CSLLAEEAIHAALWDYAEKNHILIEGLKKPKSDIHEGEEAEESY"
     gene            complement(2850..>3559)
                     /locus_tag="PLU43_02750"
     CDS             complement(2850..>3559)
                     /locus_tag="PLU43_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO27357.1"
                     /translation="PIKEIGEIAHEKGILFHTDAVQAFGQVPIDVNTYHIDMLSASGH
                     KLNGPKGIGFLYIRSGVRIRSFIHGGGQERGRRAGTENVPGIVGLGKAVERAFSIMKE
                     KTEKEAELRDYLIERVLQEVPYTRLNGDRQKRLPNNANFSFRFVEGESLLIMLDMEGI
                     CGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLTLSEETTKEEIDFTIDKIKTIVE
                     RLRNMSPLYEDFMKDNR"
BASE COUNT          929 a          943 c          641 g         1046 t
ORIGIN      
        1 cagccaatgc atatttaccc tgtgatgaat catacttaag aagatgagcc aacatcgtcg
       61 ggcttgttaa atcattaatt gctactacct cgtatccttc tgcattaaac atctgtctga
      121 atgcaagacg accgatacga ccaaaaccat taattgctac ttttactgcc atttgaaatt
      181 cctcctaaat tatatttaaa acttaagcac acgaaataaa gtgcacaaat tttatcggca
      241 gatttatttt acctaatcta tatggaaatt acaagtataa atttaactaa tcactcaaaa
      301 atatttttat tttctattgt gcaatttgat tatgatttcc ttttttatga ttatcgtgta
      361 tactattgta aaaataaaac ccgcaggagg acgtcccgtc atgaatatct caagtgattt
      421 tcatcttcat acctcttttt caagtgacag cgaaactcca atgtcttcca tgatcgaaca
      481 gggcattcgg accggcctgt cttatctgtg ttttacggaa catatggaca aagattatct
      541 gaaacggact gacgatgacc ctgattttga agtagacaca gacgcttatt taacaaaata
      601 cagggaatta aaagaaaaat acaaggacag gatcaccatt ttatttggtg tggaacttgg
      661 gctgcagccg catgtggcag attattacag gcagtacact gccgactacc cgtttgattt
      721 tattatcggc tcgtctcatc tttgccatag aaaggatccc tatttcccgg aattcttcga
      781 aggacgtgaa gaacatctcg catacaggga atattttgaa agtattctgg aaaacatcca
      841 gacctatgac ggatatgata tttacggaca cctggattac gtggttcgtt atggaccgta
      901 caaaaataaa ttctattcct atcagcgtta tgcggatatc atcgatgaga tcctgcgtgc
      961 gctgaccgct tcctccaaag gaatcgaaat caatacaggt ggttttaaat acggacttgg
     1021 gcacccaaat ccgacggaag agatcattaa acgctacaga gagcttggcg gtgaaatcat
     1081 tacaattggc tccgatgcac atgaaccaaa ctacattgcc tatgactttg ataaaattcc
     1141 atcaatactg aaaaatgccg gctttcaata ttacacgatc ttcaaagaac gaaaacccgc
     1201 attcatcaaa ttataaatcg atcagcatac gatcgtcccg ccgccgtaaa tataatcctc
     1261 ttcataaaac acaaccgcct gcccgggcgt gaccgcccgc accggctcat cgaaaacgac
     1321 cctgatacgg tcggcgtcaa gtctgtgcag cgtacaggaa accccttgat gactatagcg
     1381 gatcttagcg cgcagtcttt ttttatcttc tatttccggc actgccattg cattgatctt
     1441 atcacagatc agttctgttt tataaatatc ttcattctct ccgataacga cctcatttgt
     1501 ttccggacgg atctcccgga caaaaaccgg gtgccccatg gcaagctgca ggcccttcct
     1561 ctgtccgatc gtataatata cgacaccctt atgctttccg acgactgtcc cgtcctccag
     1621 cacaaaattg ccctctccat gcactctgtc cggtgcactt tcacaaataa accgcccgta
     1681 atcattgtcc ggaataaaac agatatcctg gctgtccggc ttattggcca cagcaatccc
     1741 gatcttttca gcaactgccc tgatctcccc ttttgtatat gctccgacag gcatcagcgt
     1801 atgggaaagc tgctcctgtg tcaggttata aagcgcatag gtctgatctt ttacagacgg
     1861 cgctgctttt aatgtaaatc ttccgtttgc aagccttgag acctgtgcat aatgtcctgt
     1921 cgcaatataa tcggcaccga tctcaaggct tctcttcaaa agggagtccc acttcacata
     1981 acggttacag gcaatacatg gattcggtgt cctgccccgc agatattcct ccgtaaaata
     2041 atcgatcacc tgctctttaa actctttcct gaaattcata acataatacg gaattccaag
     2101 aagtccggca acctgccttg catcgtcgac cgcagataac ccgcagcagc ctccgttttc
     2161 ttctttctgc atctcttctt cgtcctgcca gatccgcatc gtcgcaccga tgacctcata
     2221 tccctgttct ttcaaaaaat atgccgccac cgacgaatcc acgcctcccg acattccaac
     2281 tgcaactttt tttttcattc tgttctccag cctaccgcat tccggaaata gagatctgat
     2341 atatcatcaa taactctctt ccgcttcttc cccttcatga atatcagatt tcggtttttt
     2401 cagtccctct atcaggatat gattcttttc cgcataatcc cacagtgcgg catgaattgc
     2461 ctcctctgca agcagtgagc agtgcacctt tacagccggc agtccgtcaa gcgcttccat
     2521 caccgccttg tttgtgatct gcatcgcatc cttcactgat ttccctttca caagctctgt
     2581 cgccatactg cttgtcgcta cggccgcgcc gcagccaaac gtcttaaatt tcacgtctct
     2641 gatgatctga tcgtcgtcaa tatcaagata gatcctcata atgtcgccgc actttgcgtt
     2701 tccgaccgta ccaacaccgc ttgcgtcttc tatttccccg acatttctcg gattcgagaa
     2761 atgatccatc actttttcac tgtacatatc cgtttcctct ctttctgttt ttctgccgtc
     2821 ttcgccatat gccgcttaaa accagtttat taccggttgt ctttcataaa atcctcatac
     2881 agcggtgaca tatttcgaag cctttccaca attgtcttta ttttatctat tgtaaaatca
     2941 atttcttctt ttgttgtttc ctcacttaaa gtaagacgca gcgacccgtg agcaatttca
     3001 tgcgggagtc cgatcgcaag aagaacatga gatggatcaa gagaaccgga tgtacatgcc
     3061 gaaccgctcg aaccgcatat accctccatg tccagcatga tcagcagcga ttctccttcc
     3121 acaaaacgaa aactgaaatt cgcgttattc ggaagacgtt tctgccggtc tccgttcaac
     3181 cgtgtatatg gcacttcctg cagcacacgt tcaatcagat agtccctaag ctccgcctct
     3241 ttttccgttt tttccttcat aatggagaac gcccgttcca ctgcttttcc aagtccgacg
     3301 ataccaggta cgttttcagt tcccgcacgt ctgccgcgtt cctgaccgcc gccgtgtata
     3361 aaggagcgga ttctgactcc gcttctgata tataaaaatc cgatcccttt cgggccgttc
     3421 agtttatgcc cgcttgccga aagcatatcg atatgatatg tatttacatc gatcggcacc
     3481 tggccaaagg cctgcacggc atccgtatga aataaaattc ctttctcgtg tgcgatctca
     3541 ccaatttctt tgattggct
//
LOCUS       DAOSYE010000108         3556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18634_length_3556_cov_2.998857, whole genome shotgun sequence.
ACCESSION   DAOSYE010000108 DAOSYE010000000
VERSION     DAOSYE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18634_length_3556_cov_2.998857"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..811
                     /locus_tag="PLU43_02755"
     CDS             92..811
                     /locus_tag="PLU43_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOO27358.1"
                     /translation="MIKKIYEYLVDLLFPPRCPVCHEIVLPKGEKVCPKCRSRLYYVK
                     EPYCMKCGKPLKNDEKEFCGDCERRAHFFTEGRAVFVYDETMRKSIYQFKYGGRQEYA
                     GFYGEEIEKKLGGIIRRWNADVLIPIPLHKSRQKNRGYNQAELIAGELEKRVGIPVVQ
                     NLLVRVKKTAAQKNLNALERENNLKKAFKIKQNDVKLNSVILIDDIYTTGCTIDTAAK
                     CLREAGIKKIYYIVLSIGHGL"
     gene            823..1236
                     /locus_tag="PLU43_02760"
     CDS             823..1236
                     /locus_tag="PLU43_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HOO27359.1"
                     /translation="MNIRNCRKCGKLFNYISGQPICPACREEMEEKFQEVKQYVQNNR
                     DTSIPAVAEACDIEESQIRQWVREERLVFAEGSAFGIECESCGAMIKTGRFCDKCKAE
                     MVNSLSGAIKKPEMAKPQMKRDTRENPKMRFLDNH"
     gene            1251..1649
                     /locus_tag="PLU43_02765"
     CDS             1251..1649
                     /locus_tag="PLU43_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27360.1"
                     /translation="MLNQERIRLMTKMAAFEEIEGKKDISVNGYFRGDYISFQVLKSA
                     IYATFGFAVALLMYIIYDLETFLTDFYKMDIVAFCHDVLIRYVAVVVVYVLISYFVYS
                     YRYNKARRHIRNYGAQLRELHEMYERERQK"
     gene            1672..2619
                     /locus_tag="PLU43_02770"
     CDS             1672..2619
                     /locus_tag="PLU43_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27361.1"
                     /translation="MTNLLVIKEQLIRIYARFEAYITPVLKFFLMLFSLIIINRNIGF
                     MNKIKNPAIVLIVALLSTFLPLNVMILLAAAFIILHMYALSLECAAVVGLVFLLMFVL
                     YFRFTPKDSVAVLLTPICFALKIPYVIPLSMGFVGGPLSCISVSCGVVVYYILNYVKN
                     NSEQLVGSEDAENALSGFKYVIDSLIKNDTMFLMVIAFSITVILVYMIRRLSINYSWQ
                     IALGAGAIINLLVILIGSVSMDTDISIPGVIFGLFVSVLFVLVLQFFVFNVNYSRTEY
                     VQFEDDEYYYYVKAIPKLSMEAPQVRVKRINPQKNPHRN"
     gene            2656..3510
                     /gene="cdaA"
                     /locus_tag="PLU43_02775"
     CDS             2656..3510
                     /gene="cdaA"
                     /locus_tag="PLU43_02775"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOO27362.1"
                     /translation="MEQIIEKFTTFSERYLTWLSMPSIMWTDIVEIIIISFLVYHVLV
                     WIKNTKAWSLMKGVIVILVFVLLAAIFSMTTILWIAKNIFSVAIMAMVVVFQPELRKA
                     LEQLGRKNVLASLFQFDSMRVSGELFSDKTINEIIRASFEMGRAKTGALIVIEQNDPL
                     IDYERTGIILDSVVSSQLLINIFEHNTPLHDGAVVIRENRIVSATCYLPLSDNMELSK
                     ELGTRHRAGVGISEATDSLTIIVSEETGRVSVAVGGELFRNVDADFLRIKLQLIQNKV
                     VEEKKFRF"
BASE COUNT         1069 a          566 c          883 g         1038 t
ORIGIN      
        1 gaacagatcg ggcggatgat cgaaaatgag gacgaacaaa agcggtttac cgatctttcg
       61 gaaaaaatca gggagatgaa aggaatggcg gatgattaaa aaaatatatg aatatctggt
      121 tgatcttctc ttcccgccaa ggtgtccggt atgtcatgag atcgttttgc cgaaaggaga
      181 aaaggtctgt ccaaagtgcc gttcgcggct gtattatgta aaagagccgt actgcatgaa
      241 gtgcgggaaa ccgttaaaga acgacgagaa ggaattttgc ggtgactgtg aaaggagggc
      301 gcattttttt acggaagggc gtgcagtttt tgtttatgat gaaaccatga gaaaaagtat
      361 ttatcagttc aaatacggcg gcaggcagga gtatgccgga ttttatgggg aagaaattga
      421 aaaaaaactt ggcggtatca tacgcagatg gaatgcggat gtattgatac cgattccttt
      481 acataaaagc agacaaaaga acagggggta taatcaggcg gaactgattg caggagaact
      541 ggaaaagagg gttggaattc ctgttgtgca gaatttgctt gtcagggtaa aaaaaacggc
      601 tgcacagaaa aatttaaatg ccttggaacg cgaaaataat ttgaaaaagg ctttcaaaat
      661 aaagcaaaat gatgtaaaat taaattcagt catattgatt gatgatattt atacgacggg
      721 atgtacaatc gatacagctg caaaatgtct tagagaagca ggaataaaaa aaatatacta
      781 tattgtctta agtatcggac atgggctata ggaggaattg agatgaatat caggaattgc
      841 agaaaatgcg ggaaattatt taattacata tcaggtcagc cgatttgtcc tgcctgcagg
      901 gaagaaatgg aagagaagtt tcaggaagtg aaacagtatg tacagaacaa cagggatact
      961 tccataccgg cagtggctga ggcctgtgat attgaagaaa gccagatcag gcagtgggta
     1021 agggaagaga gacttgtatt tgcggaaggt tcggcttttg gaatcgaatg tgagtcatgc
     1081 ggcgcaatga ttaaaaccgg tcgtttttgc gataaatgca aagcagaaat ggtaaattca
     1141 ttatcaggag cgattaaaaa acctgaaatg gcaaagccgc agatgaaacg ggatacacgt
     1201 gaaaatccca aaatgcgttt tttggataat cattaaaggg acgatatatt atgctgaatc
     1261 aggaacggat caggctgatg actaaaatgg cggcctttga agaaatagaa ggaaaaaaag
     1321 atatttctgt aaatggttat ttcagagggg attatattag ctttcaggta cttaagtccg
     1381 caatttatgc cacgtttggc tttgcggtcg ctcttttgat gtatattata tatgatttgg
     1441 aaacatttct gactgatttt tataagatgg atattgttgc gttctgtcat gatgttctca
     1501 tccgttatgt cgctgttgta gtagtgtatg tattgatttc ctatttcgta tattcctatc
     1561 ggtataataa agcgagaaga cacatcagaa attatggagc acagcttcgc gaattacatg
     1621 aaatgtatga acgcgaacgt caaaagtgac gcggaaagga ataacgggac gatgacaaat
     1681 ttacttgtta taaaagaaca gctgatcagg atctatgcca ggttcgaggc atatatcacg
     1741 ccggttttga aattttttct gatgctgttt tcactgatca ttattaacag gaacatagga
     1801 tttatgaaca aaataaaaaa tccggccata gtactgatcg ttgctctgtt aagtacgttt
     1861 ctgcccctga atgtcatgat tttgctggcg gcggcattta ttattttaca tatgtatgca
     1921 ctttccctgg aatgcgcggc ggttgttggt ttagtgtttc tgctgatgtt tgtgctttat
     1981 ttcagattta caccaaagga ttctgttgca gtgcttctga caccgatctg ttttgcactg
     2041 aagatccctt atgtgattcc gctttccatg gggttcgtag gaggtccgtt gtcctgtatt
     2101 tcggtaagct gcggtgttgt tgtctattat atcctgaact atgtgaaaaa taattccgaa
     2161 cagcttgtcg gcagtgaaga cgcggaaaat gcactgtctg gatttaaata tgtaatagac
     2221 agtctgataa agaatgatac gatgtttttg atggttattg ccttttctat tacggtaatt
     2281 ctcgtttata tgatcaggcg cctgtcaatt aattattcat ggcagatcgc gcttggcgcc
     2341 ggagcaatca tcaacctgct ggttattctt atcggcagtg tttccatgga tacggatatc
     2401 agtattccgg gagtgatttt cggattgttc gtatctgttt tgttcgtttt agtcctgcag
     2461 ttttttgttt ttaatgtaaa ttattccagg acggaatatg tacagtttga ggatgacgaa
     2521 tattactatt acgtcaaggc gatccccaaa ttgtcaatgg aagccccgca ggtaagggta
     2581 aaaaggatta atccgcagaa aaatccgcac agaaattaag agcggaaata ttgagagttg
     2641 gtttgggaag aagtaatgga acagattatt gaaaagttta ctacattttc agaaagatat
     2701 ttgacgtggc tttccatgcc aagcattatg tggacggaca tcgtggaaat tattattatt
     2761 tcctttcttg tttatcatgt gcttgtatgg atcaaaaata caaaagcctg gtcattgatg
     2821 aagggcgtca ttgtaattct ggtctttgtc cttttggcgg caattttcag tatgacaacg
     2881 atcctctgga ttgccaaaaa tatattcagt gtagcaatca tggcaatggt cgtcgtgttt
     2941 cagcccgagc ttcgaaaggc gcttgagcag ctcggccgga aaaatgtgct tgcttcgctt
     3001 tttcagtttg acagtatgcg cgtttcagga gaacttttca gtgataaaac gatcaatgag
     3061 atcatcaggg caagttttga gatgggacgg gcaaagaccg gtgcattgat cgtgatcgag
     3121 cagaatgatc cgctgatcga ttatgaaaga acagggatta ttcttgattc tgttgtttcc
     3181 agccagctat tgatcaatat ttttgaacat aatactccgt tgcatgacgg agctgttgtt
     3241 atacgcgaaa acaggatcgt gtcggcaacc tgttacctgc cgctttccga taatatggaa
     3301 ttaagcaagg aactgggaac aaggcatcgc gcaggagttg gtatttcaga ggcgacggat
     3361 tcccttacga tcatcgtatc ggaggaaaca gggagagtgt cggtcgctgt gggcggtgag
     3421 ctttttagaa atgtcgatgc ggattttctg cgcataaaat tgcagctgat tcagaataaa
     3481 gttgtggaag aaaagaaatt ccgcttctga aaaggcaggt cgaagaatga aaagcaagct
     3541 gacaaataat atcggt
//
LOCUS       DAOSYE010000109         3552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18671_length_3552_cov_3.083214, whole genome shotgun sequence.
ACCESSION   DAOSYE010000109 DAOSYE010000000
VERSION     DAOSYE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18671_length_3552_cov_3.083214"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3552)
                     /locus_tag="PLU43_02780"
     CDS             complement(<1..>3552)
                     /locus_tag="PLU43_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HOO27363.1"
                     /translation="GKTYVLHEITSPAGYGVAGDIVFTINEDGSITTSARTSDSGNGM
                     TLIMEDPIISVYISKEELGTTTELSGASLAISDSTGIIEEWQTNGQSHKITAALKVGE
                     VYTLEETKAPAGYYTAEPVTFTILSNGTLQITKDSSAKSSAMSYPSEYGTSVVKNFLT
                     MYDRPIRVEISKKRLTGGDDDYVEGAELALYGKDDSGEYNTLIYTWTSPASGAVLVPY
                     GKLEVGESYKVVETSVPAGYVKAADVIFTVKDYDEFTQTDEDGNVTQEEVVLDGMTSV
                     IISKQSVSDTEELAGAELKVTDEDGNEIASWTSGTKPVLLSTLASESELSAEEKTNYS
                     GYTIIYGVSLEAGETYTLTEVTAPDGYAVAQSVEFTVSEEGTTSPSPVVMKDAPLEIQ
                     LSKKDLTTQDNLSGAELQLLDKDGSVLHEWTSGEKPVLFTQRTLSASEAAAYDEVVKL
                     VLIETKDETDPYTYTLHEVSAPTGYATADDITFTIKGTDVQQEDGSIREESMYDPRDG
                     EVSLSGTKTWIIPKDAEGNPLANYTYPTVTIELYRDSTTQGVMDTTAYKSIDLTNGEE
                     NYTFGPLEKYKYKDAGIDYEYTYTVKEVMSTAAAKQFTSEEVEDEDGNITGFINRLKQ
                     EKTSLSGEKVWILCRDEDGNIDTSQDYADVNVYLLRDGSRVDADGDGTVDYVTIKNGA
                     VSTDGTESFSFSDLDKYDLDTGREYTYTVEEVGDGSTVFDNDITYREHECQILNVPFH
                     DPFSISGKKLWIDPVGSERPDVTIELYRDGKLFKTTKLNADNTFDFGDLYEYNLGWSD
                     DEDDNKATADGHKFVYELKETGATGYSFEVDFNGEIRTLTSGEQVSEATVTNTITQEY
                     VEISGTKYWDDAGDSSSRPTATINLYATDTAGRSHTLVDTYYLSNSSTTYSFGTTGRV
                     KLPKYDTNGQVITYTVEEEALSGYVSSQSGYDFTNTPSKVRISKVDATTGDELAGAYM
                     KLTRVSTGTVVDSWTSTTTAHYIEGLVMGESYTLSESLAPLGYEIASSVTFTVRTDGV
                     EQTVTMYDEPITGTVTLTKRDTTTRALLTGAVFNLYTSSGTLVRATGSAGSYTYSTSG
                     TTSLAVSSSGTLSVYGLPYGSYYFKEITAPSGYTLSTATESFTIGEADASVSVTFLDT
                     RSTGAVGLIKTNLGGTTALSGAVFELYSRTPRTVGQAA"
BASE COUNT          853 a          931 c          671 g         1097 t
ORIGIN      
        1 gcagcctgtc cgaccgttct cggtgtcctt gaataaagct caaagactgc tcccgaaagt
       61 gctgttgtgc ctcctaaatt tgtcttgatc agaccaacgg ctccggtgct ccttgtatca
      121 aggaacgtca cagacactga tgcatccgct tcaccgatcg taaatgattc tgtcgcagta
      181 cttaacgtat aaccgcttgg tgccgtaatt tccttaaagt aataggagcc atacggcaat
      241 ccgtatactg ataatgtacc acttgaagat actgccagcg acgtcgttcc tgaagttgaa
      301 tacgtgtagg aaccggccga acctgttgcc ctcacaagtg tccctgatga agtatacaga
      361 ttaaataccg cacctgtaag aagtgccctt gtcgtcgtat ccctcttagt cagcgtcacc
      421 gtacctgtaa tcggttcatc atacatcgtt actgtctgct caacaccgtc cgtacgaacc
      481 gtaaatgtta cggaacttgc aatttcatag ccaagcggtg caaggctctc cgacaatgta
      541 taggattctc ccattacaag tccttcaata taatgtgctg tcgttgtcga agtccagctg
      601 tccactactg ttccggtgct tacccttgtg agtttcatat atgcaccggc aagctcatca
      661 ccggttgttg catctacctt tgaaatacga actttactcg gtgtatttgt aaaatcatag
      721 ccgctctggc tggaaacata gcctgaaagt gcttcttctt ccaccgtata ggtgatcacc
      781 tggccgttcg tatcgtactt aggcagtttt actcttcctg tcgttccgaa gctgtatgta
      841 gtactcgaat tactcaaata ataagtatct accaaagtat ggcttcttcc cgccgtatcg
      901 gtcgcataca gattgatcgt agccgtcgga cggctgctgg aatcaccagc atcatcccag
      961 tattttgtac cggaaatctc aacatattcc tgagtaatcg tattggtaac agttgcttca
     1021 gaaacctgtt ctcccgatgt caaagttctg atctctccgt taaagtcaac ctcaaatgaa
     1081 tatcctgttg cgcctgtttc ttttaattca taaacaaatt tgtgaccatc tgccgttgcc
     1141 ttattatcat cttcatcgtc gctccatcca agattgtact cgtacaggtc gccaaaatca
     1201 aatgtattgt cggcattcag ttttgtagtc ttaaataact taccgtctct ataaagctcg
     1261 atcgttacat ctggcctctc ggaaccaacc ggatcgatcc agagcttctt accgctgata
     1321 ctgaacggat catggaacgg cacatttaat atctgacatt catgttctct gtaagtaata
     1381 tcattatcaa atactgtgct gccgtcgccg acttcttcaa ccgtataggt atattccctt
     1441 cctgtatcca gatcatattt atccagatca ctgaatgaga agctttccgt accatctgtc
     1501 gaaacagcac cgttcttgat cgtaacataa tcaacagtac cgtcgccgtc cgcatcgact
     1561 ctgcttccgt ctcttagcag atagacattc acatctgcat aatcctgact tgtatcaata
     1621 ttgccgtctt cgtctctgca gagtatccag actttttcgc cgctgagaga agtcttctcc
     1681 tgcttcagtc tgttaataaa tcctgtgatg tttccatctt catcttctac ttcttcgctt
     1741 gtaaactgct tggccgccgc tgtagacatg acttccttaa ccgtataggt gtactcgtaa
     1801 tctatgcctg catctttata tttatatttc tccaacggac cgaatgtata attctcttca
     1861 ccgtttgtaa gatcgatcga tttatatgcg gtcgtatcca tcactccctg tgtcgtggaa
     1921 tcacgataca gctcgattgt aactgtcgga tacgtgtagt ttgcaagcgg attcccttcc
     1981 gcatcctttg gaatgatcca tgtcttcgta ccacttaacg atacttctcc gtctctcgga
     2041 tcatacatgg actcttcacg gatacttcca tcctcctgct gcacatccgt acccttgatc
     2101 gtaaaggtaa tatcgtccgc agttgcataa ccggtaggtg cagacacttc atgcagtgta
     2161 taggtataag gatccgtttc atcctttgtc tctatcaata ccagttttac gacttcgtca
     2221 tatgccgcag cttccgatgc agataatgta cgctgtgtaa acaatacagg cttttctcct
     2281 gatgtccatt catgaagcac acttccgtcc ttatcaagaa gctgtaattc tgctccggaa
     2341 agattatcct gcgtcgtcag atccttcttg gaaagctgga tctcaagcgg cgcatctttc
     2401 atcactaccg gcgaaggaga ggtcgttcct tcctcactta ctgtaaattc aacgctctgt
     2461 gccacagcat agccatccgg tgccgtcacc tcagtcaatg tatatgtttc tcctgcttca
     2521 agagatacac cgtaaataat tgtataaccg ctgtagtttg tcttttcttc tgcggaaagt
     2581 tccgactctg aagcaagtgt tgataatagt acaggttttg ttccggacgt ccatgatgca
     2641 atttcgttac cgtcttcatc cgtaaccttc aattctgccc cggcaagctc ttccgtatcg
     2701 gaaacagact gctttgaaat aatcacactt gtcataccat ccagtacaac ttcttcctgt
     2761 gtaacatttc catcttcatc cgtctgtgta aattcatcat aatcctttac ggtaaaaatc
     2821 acatcggcag cctttacata accggccgga acagaagtct ctaccacttt gtagctttcg
     2881 cctacttcca gcttgccgta aggtaccagt accgcaccgc ttgccggaga tgtccatgta
     2941 tagatcagcg tgttgtattc accgctgtca tccttgccgt acagagcaag ctctgcacct
     3001 tccacataat cgtcgtctcc tcctgtcagc cgtttctttg atatttctac ccgaatcggt
     3061 ctgtcataca tcgtcaggaa attcttaaca accgaagtcc catattcgga tggataagac
     3121 attgcagaag atttagcaga agaatctttt gtaatctgca gcgtgccatt tgacaaaatc
     3181 gtaaatgtca caggttctgc cgtatagtat ccggccgggg cttttgtctc ttccagtgta
     3241 taaacctcgc ccactttcaa tgctgccgtg atcttatgag actgtccgtt cgtctgccac
     3301 tcctcaataa tacctgtact gtcactgatt gcaagagatg ctcctgaaag ctccgttgtc
     3361 gtaccaagtt cttctttgga aatataaaca ctgattatcg gatcttccat gatcagcgtc
     3421 attccgttgc cgctgtctga tgttcttgca cttgtcgtaa tactgccgtc ttcattgatt
     3481 gtaaatacaa tatccccggc tactccatag cctgcaggtg atgtaatctc atgcagtaca
     3541 taagtctttc cg
//
LOCUS       DAOSYE010000110         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18767_length_3538_cov_2.954350, whole genome shotgun sequence.
ACCESSION   DAOSYE010000110 DAOSYE010000000
VERSION     DAOSYE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18767_length_3538_cov_2.954350"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PLU43_02785"
     CDS             <1..1504
                     /locus_tag="PLU43_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27364.1"
                     /translation="LASNLQGMSDNMGYLNSEMNTASDVLLADFEAVNDQFNVIMLLY
                     TDSIDGVLDMDYTASYEDDSNEVAQECTDATIAECTNNGEVNGDIDASGIAGTMAIEY
                     DFDLESDETGIKDAKANSTYRAKCVMRKDKNNGTVTAQKSYAGGVTGLQEMGTILLCE
                     NYGRIVSNAGDYVGGVAGQSLSYIQKSYAKCSLSGGTYVAGITGSGTNITDCYAMVHV
                     SEAEAFFGAIAGEITEDGQVHHNLFVGSDLAGIDRISYSGKAEPVSYEALLEKSDVPA
                     GFRKLTITFYCDDAVIKKEDCRYGDSIPDSDYPQIQEKEGYYVDWDIDDLSNVTFDQD
                     VTAQYMRYLTTLAGSQTRGNRQSDLLVDGTFEEGDELHATLYSTSSLPMKNVLEHWKL
                     EFPEDGNAQHLLRYQAPDNQTEDVEIYVKNGETWGKEETETMGSYQIFTIDGTAAEIA
                     IVSKTKSLTDYIWIAAVVCGAVTVMIIYSNKKKKHKKKKKNGKTLEEIEI"
     gene            1531..2940
                     /locus_tag="PLU43_02790"
     CDS             1531..2940
                     /locus_tag="PLU43_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27365.1"
                     /translation="MKNRSRKTKKNSWGPVTAAVLILAVLPVLGGCKKQNSGEAAEAE
                     TPVEEESTAAAKELDTDSLPANLQKMVPLMDALNLALCNYDASYDASDAEFVWNSILL
                     AAVNSDWEEYGLSMSEDGMTLLVPKEVTEEIAFAMFADLSELPEVPESLSQAEDGVPA
                     VSVNENGDYTFIVGDRGLSAYRAESFTDNGDGTYTATISLNGAGTEQNEIISTVYTLR
                     ERASEKDDPLFSYAVAAGEPADQLTEDKIAGIPYLDVYMQAYGCELYDESDLMYESVE
                     EIPWFSCFNTEDDTCASLNDRIREEILSVDEQAFEEEGIWPDIRSYFFTDDTYLQAVS
                     TVITYPNYATDGAVYSYNYDKKNKCAMTNEDGLKLAGVTEEALYEMTESAYEPENDGD
                     VYDHAQLCGFRVKEDGTVDFYMELFIDNELADDYDRIAVYQMSDSTLHFYDGQILIPD
                     SETDTMDPPFTHGNYDEKS"
     gene            3041..>3538
                     /locus_tag="PLU43_02795"
     CDS             3041..>3538
                     /locus_tag="PLU43_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO27366.1"
                     /translation="METRIEITEAVREEFDHIVQERLIYSVYQPIVSFETGEIFGYEG
                     LSRIKKENSILNIGELFQAAEQMNKVWELEALCRRRTLKNAVKKPCGVKLFLNVDPNC
                     IRDEKFKSGLTTKYLKQYGLHPSDIIFEITERGAIFDAEAFKNTIFHYRQEQFEIAID
                     DFGTGY"
BASE COUNT         1073 a          668 c          968 g          829 t
ORIGIN      
        1 ccttgcatca aatctgcagg gaatgtccga caatatggga tatctgaaca gtgaaatgaa
       61 tacggcaagt gatgtgctgc ttgcggattt tgaggcggta aatgatcagt tcaatgtgat
      121 catgctgctc tatacggact cgatcgacgg tgttcttgac atggactata cggcgtctta
      181 tgaagatgat tccaatgaag tcgcacagga gtgtaccgac gcgacaatag cggaatgtac
      241 gaataatgga gaagtaaacg gagatatcga tgcttccgga attgccggaa caatggcgat
      301 cgagtacgac tttgatcttg aaagcgatga gacagggatc aaggatgcga aagccaattc
      361 cacttatcgg gcaaaatgcg ttatgagaaa agataaaaac aacggaacgg tcacagctca
      421 gaaaagctat gccggcggcg ttaccggact gcaggagatg ggaacgatac tgctttgtga
      481 aaattacggg aggatcgtaa gcaatgccgg ggattatgtc ggaggtgttg ccggtcagtc
      541 cctttcctac atacagaaaa gctatgcaaa gtgttctctg tccggcggaa catatgtggc
      601 agggattacc ggttcaggaa cgaatattac ggattgttat gcaatggtgc atgttagtga
      661 ggcggaggca ttttttggag cgatcgccgg cgagatcaca gaagacggcc aggtgcatca
      721 taatctgttt gtcggaagcg atctggcggg aatcgaccgt atcagctaca gcggaaaggc
      781 agagccggtc tcctatgagg cactgcttga aaaaagtgat gtgccggccg gttttcggaa
      841 gctgacgatc acattttact gtgatgatgc ggtaataaaa aaagaagact gccgttacgg
      901 agattcgatc ccggattccg attatcctca gatacaggaa aaagaagggt actatgtcga
      961 ctgggacatc gatgatctaa gcaatgttac gtttgatcag gatgtaacgg cacagtacat
     1021 gcgttatctg acgacgctgg caggcagcca gacaagagga aaccggcagt cagatcttct
     1081 tgttgacgga acgtttgagg aaggagacga actgcatgca acgctttatt cgacttcctc
     1141 gctgcctatg aaaaatgtgc tggagcactg gaagctggag tttccggagg acggcaatgc
     1201 acagcacctc cttcgctatc aggcgcccga taatcagacg gaggatgtag agatctatgt
     1261 aaaaaacgga gaaacatggg gaaaagaaga aacagaaaca atgggcagct accagatatt
     1321 cacgatcgac ggtacggcag cagagattgc gatcgtttca aagacaaaga gtttaacgga
     1381 ttatatctgg atcgcggctg ttgtatgcgg cgctgtgaca gtgatgatca tttattccaa
     1441 taaaaagaaa aaacataaga aaaagaaaaa aaacggaaaa acactggaag aaattgaaat
     1501 atgatataaa aagcagggag ggatacaatt ttgaaaaaca gaagcagaaa aacaaaaaag
     1561 aacagttggg gaccggtgac ggcagcagtg cttattttag cagtactgcc tgtccttggc
     1621 ggatgcaaaa agcaaaacag cggggaagca gctgaggcgg aaacaccggt ggaagaggag
     1681 agtacggcag ctgcaaaaga acttgatacg gacagccttc cggcgaatct gcagaagatg
     1741 gttccgctga tggacgccct gaatcttgcc ttatgcaatt acgatgcttc ctatgatgcg
     1801 tccgatgcgg aatttgtctg gaacagtatc ctgcttgctg cagtcaattc cgactgggag
     1861 gaatatggac tgtccatgtc tgaagacggt atgacactgc ttgtgccaaa ggaagtgaca
     1921 gaagagatcg catttgccat gtttgccgat ttgtcggagc ttccggaggt tccggaaagt
     1981 ctttcacagg cagaggacgg agtaccggct gtcagcgtga atgaaaacgg agattacacg
     2041 tttatcgtcg gggacagagg actgagtgct taccgggccg aaagctttac cgacaacgga
     2101 gacggcacct atacggcgac gatatctctg aacggtgcag gaacagagca gaatgaaata
     2161 atcagcaccg tttatacgct cagggagcgc gcatctgaaa aagacgatcc gctgttttct
     2221 tatgcggtag ccgcaggcga gccggccgat cagctgacag aagacaaaat agccgggatt
     2281 ccgtatctgg atgtttatat gcaggcatat ggctgtgaac tctatgatga aagcgattta
     2341 atgtatgagt ccgtagagga gattccctgg ttttcctgct tcaatacgga agacgatacg
     2401 tgtgcttccc taaacgaccg gatcagggaa gaaatactca gtgttgatga gcaggctttt
     2461 gaggaggaag gaatatggcc ggatatcaga tcgtatttct ttacggacga cacctatctg
     2521 caggcagtat ccacggtaat tacgtatccg aattatgcaa ctgacggagc ggtctattcg
     2581 tataattatg ataaaaaaaa taaatgtgcc atgacgaatg aggacggtct gaaactcgca
     2641 ggcgttacgg aggaggcact gtacgagatg acagagtctg cctatgaacc ggaaaatgac
     2701 ggggatgtat atgatcatgc acagctttgc ggatttcgtg taaaggaaga cgggacagtc
     2761 gatttttata tggaattatt tattgacaat gagctggcag atgattacga tcggatcgca
     2821 gtctatcaga tgtccgattc cacactgcat ttttatgacg gacagatact gatcccggat
     2881 tcggaaaccg acacgatgga tcctcctttt acacacggaa attacgatga aaaatcttga
     2941 tttttcataa atcatacata actttacctt attttgatag aatacttttc attcctttac
     3001 taatctgaaa gagtgaaaat cagcggagtg aggtaagcgt atggagacaa gaatagaaat
     3061 tacggaggct gtcagagagg agtttgatca tattgttcag gaaagactga tctattcggt
     3121 ctatcagccg atcgtatcgt ttgagaccgg tgagatattc ggctatgaag ggctgagccg
     3181 gataaagaag gaaaacagta tcctgaatat cggagaatta tttcaggcgg cagaacagat
     3241 gaataaggtg tgggaactgg aagcactgtg caggcggcgt actttgaaaa acgcggtaaa
     3301 aaaaccatgc ggggtaaagc tgtttttgaa tgttgatccg aactgtatca gggacgagaa
     3361 attcaaaagc gggctgacga caaaatattt aaaacagtac gggctgcacc cttcggatat
     3421 tatatttgaa attacggaaa gaggtgcgat ttttgatgcg gaagcattta aaaatacgat
     3481 ttttcattat cggcaggagc agtttgagat cgcgatcgat gattttggca ccggttat
//
LOCUS       DAOSYE010000111         3535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18790_length_3535_cov_3.673563, whole genome shotgun sequence.
ACCESSION   DAOSYE010000111 DAOSYE010000000
VERSION     DAOSYE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18790_length_3535_cov_3.673563"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2142)
                     /locus_tag="PLU43_02800"
     CDS             complement(112..2142)
                     /locus_tag="PLU43_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27367.1"
                     /translation="MLAVILPVIIISMSVLTIISARSSQNIIQEQVADAMDSAIDAQM
                     NNITKELAVVEKSSMDLSRLVGLTYSVEELSTYEKILQKIADDNPLILGCGIWFEPYV
                     YDADKKYVGPYVYKDGDSFVTTYDYESEEYDYLNQEYYLNVANGETAPVFTSPYYDEG
                     MGMTLSSCSMPIFDDADNFIGVITVDIDLTSIQDIVKSIQIGTSGQAMMITGSGTYMS
                     CEDNEKVETQLNIAEDENTSLANAASEIIGTEKGITSYVKGDEQYNLYYDTIDGVGWK
                     LIVEMSQAELNKPVESLIYKLILVCILAVICSILAVLLVVTSISRNLKKVKVFAGLLA
                     EGNFTIDSLEVKSKDELGMMGVSLNDMYENNRAVIQNISKHSETIRLSSTKLSTSSKE
                     LFDRFMKIEEYMSKVNEAMMSSSAATEEVNASVEEVTSSVNILSEETVKSQKLVDEIR
                     VRADKIEEDSQHSFDHASQLSSQFEKSLSKSIENAKIVESIGTLASVISNIAEQINLL
                     SLNASIEAARAGEQGKGFAVVASEIGKLAGETSKAIEEIQHTIIEVQEAFNQLTGESS
                     ALLKFLSETVTPDYHSFTDVAKQYGADAASIEKLSGRISVMSEDIERIMGEVSSAIQN
                     IAESSQDTAENSSKTMNAVNEVAVVVDSVSIMSKEQQDISTELSSVVGKFNI"
     gene            2456..>3535
                     /locus_tag="PLU43_02805"
     CDS             2456..>3535
                     /locus_tag="PLU43_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO27368.1"
                     /translation="MSSEIRDISLAESGERKIEWVKRNCDLLRTLEKEFGETKPFAGK
                     KIALSVHLEAKTAYLCKVLAAGGADMYVTGSNPLSTQDDVAAALVKAGLEVHAWYNST
                     AEEYDRHIEAVLSPGPNIIIDDGGDLVHMMHTKLTHLIPHVIGGCEETTTGILRLIAM
                     DKADELKFPMVLVNNADCKHFFDNRYGTGQSVWDGINRTTNLIVAGKIVVVAGYGWCG
                     KGVAMRAKGLGARVVVTEIDPIKAIEAVMDGFDVMPMNEAAKTGDFFVTVTGCDKVID
                     EEDFAVMKDGAILCNAGHFDCEIDMARLREIAVDTKEMRKNIMGYQLPTGQWIFILAE
                     GRLVNLAAGDGHPAEIMDMSFAIQAL"
BASE COUNT          965 a          732 c          788 g         1050 t
ORIGIN      
        1 cgggcggatt ctggactttc accagttaga aacgtgcgcc gccaggcgca ctaaagaaaa
       61 aaaaagccgc gggatcttta tcctgcggct tttcttgaaa gatggatcat atcatatatt
      121 gaatttgcct acgacactgc ttaattcggt agaaatatcc tgctgttcct ttgacataat
      181 tgatacactg tccacaacga ctgcaacttc atttacggca ttcattgttt tactgctgtt
      241 ttcagctgta tcctgtgagg attcggcgat gttctgaatt gcagaactta cttcgcccat
      301 gatcctttcg atatcttccg acatgacaga gatcctgccg gacaattttt caatggaagc
      361 agcgtctgct ccgtattgct ttgccacatc tgtaaagctg tgataatcag gagtgactgt
      421 ttcagacagg aattttaata atgctgatga ttctcctgtt aactgattga aagcttcctg
      481 tacttcaatg atcgtatgct gtatttcttc gatcgctttg gaggtttctc ctgccagttt
      541 accaatttca cttgcaacga ctgcaaatcc ttttccctgt tcgcctgctc ttgctgcttc
      601 aatggaagcg tttaatgaaa gcaggttgat ctgttcggct atattggaga taacgcttgc
      661 cagtgtaccg atcgattcca cgatctttgc attttcaata ctcttgctca gactcttttc
      721 aaactgactg gaaagctgcg aagcatggtc aaaggaatgc tggctgtctt cttcgatctt
      781 gtcggctcgt acacggatct catcaaccag tttctgactt ttgacggttt cttccgataa
      841 tatattgaca gaagaggtta cttcttcaac agaagcgttt acttcttctg ttgcggcgct
      901 cgaagacatc atggcttcgt taacttttga catatattct tcgatcttca taaaacgatc
      961 aaataattcc ttggaagaag tgcttaattt agtactggac aggcgaattg tttccgaatg
     1021 ctttgaaata ttctggataa ccgcccggtt gttctcgtac atatcattca gggacacgcc
     1081 catcatgcct aattcatctt tggacttaac ttccagcgaa tcgatcgtaa aattgccttc
     1141 tgccagcaga ccggcaaaca ccttgacctt tttcaggttt ctggagatcg aggtaactac
     1201 aagcaaaaca gccaggatag agcagatcac agctaaaata catacaagga taagtttgta
     1261 aatgagactt tccacaggtt tgttcagctc tgcctgcgac atttcaacga tcaacttcca
     1321 gccaacgcca tcgatcgtat catagtagag attatattgt tcgtctcctt ttacatagga
     1381 ggtaattcct ttttccgttc cgatgatttc cgatgccgca tttgcaagag atgtattttc
     1441 atcttccgca atattgagct gagtttctac tttttcatta tcctcacaac tcatatatgt
     1501 accggagccc gttatcatca tagcctgtcc gcttgttccg atctgaatgg attttacgat
     1561 gtcctgaata gaggtcaggt caatatcgac ggtaatgaca ccgatgaaat tgtcggcatc
     1621 atcaaaaatc ggcatggaac aggaagaaag cgtcattccc atgccttcgt cgtaataggg
     1681 cgacgtaaat accggtgccg tctctccgtt agctacgttt aaatagtatt cctgatttaa
     1741 ataatcatat tcttcagatt cgtaatcata ggtggttaca aaagaatctc cgtctttgta
     1801 aacataggga cccacatatt ttttgtctgc gtcatataca tatggctcaa accatatgcc
     1861 gcagcctaag atcagcggat tatcatcggc gatcttttgc agtatttttt cataggtgct
     1921 taattcttcc acagaataag taagaccaac gagtcttgac agatccatgg agcttttttc
     1981 aacaactgcg agttcttttg taatattgtt catctgagcg tcgatcgcgg aatccatagc
     2041 atccgcaacc tgttcctgaa taatattctg actggatcgg gcactgatta ttgttaaaac
     2101 actcatggaa ataataatta ccggaaggat aaccgccagc attttaatac tgattttttt
     2161 tctttttcct ttttcttttt ttcgtgtttt ttcatagata atcctttcca catattattt
     2221 tgtatacatt aaacatttaa acaaattaat catagcatat tagcctttga aaaacaatga
     2281 attgccagta ttaaaattgt aaaatgattg acattcataa aaccagccgg tatagtaaaa
     2341 atgtgccggc acagcaggtt tttgtataaa agcgggcgcc agggtgatga caggacagac
     2401 gaccatcaca ttaataaaaa ttcggctgaa attactactt cggaggaaat tatatatgag
     2461 cagtgaaatc agagacatca gcctggccga gtcaggtgaa agaaaaattg agtgggtaaa
     2521 aagaaattgt gaccttttga ggactcttga gaaagagttt ggcgaaacaa agccgttcgc
     2581 cggcaaaaag attgcactgt ccgtgcatct ggaagcaaaa acagcctatt tatgcaaggt
     2641 tttggcggca ggcggggcag atatgtatgt aactggctcc aatccgcttt ccacacagga
     2701 cgatgtggcg gcagcacttg taaaagccgg ccttgaagta catgcgtggt acaattccac
     2761 ggcagaggaa tatgacaggc atattgaagc cgttctttct ccgggaccaa atattattat
     2821 tgatgacggc ggagatcttg tgcatatgat gcatacaaag ctgacgcatt tgattccgca
     2881 tgtgatcgga ggctgtgaag aaacgacgac cggtatttta agactgatcg caatggataa
     2941 agccgatgaa ctgaagtttc caatggtgct tgtcaataat gcggactgca agcatttttt
     3001 tgataaccgg tacggaacag gccagtctgt ctgggacggt attaaccgta cgacaaatct
     3061 gatcgtggcc ggaaagatcg tagttgtggc aggatatggc tggtgcggca agggtgttgc
     3121 aatgcgggca aaaggacttg gtgcccgggt tgttgtgaca gagatagatc cgataaaggc
     3181 aatcgaggcg gttatggatg gatttgatgt tatgccgatg aacgaagcgg ctaagaccgg
     3241 agattttttt gtgacggtaa caggctgtga taaggtgatt gatgaggaag atttcgcagt
     3301 gatgaaagac ggcgcgatcc tgtgcaatgc gggacatttt gactgtgaga tcgatatggc
     3361 gcgtctgcgc gagatcgcag ttgatacaaa agaaatgaga aagaatatta tgggctatca
     3421 gctgccgacc ggccagtgga tctttattct ggcagaaggc cgtcttgtca atctggcagc
     3481 aggtgacgga catccggcag agatcatgga tatgtctttt gccattcagg cactt
//
LOCUS       DAOSYE010000112         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18918_length_3516_cov_2.774921, whole genome shotgun sequence.
ACCESSION   DAOSYE010000112 DAOSYE010000000
VERSION     DAOSYE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18918_length_3516_cov_2.774921"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLU43_02810"
     CDS             complement(<1..309)
                     /locus_tag="PLU43_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO27369.1"
                     /translation="MELRHIRYFLAVAENLNFTKAAKQLCIAQPPLSRQIQDLEKELG
                     TLLFIRTPHHLQLTPEGRIFKQDAKKILALVSKSAEEVKDAKEGIGGTLYLGSVEGHG
                     P"
     gene            652..1239
                     /locus_tag="PLU43_02815"
     CDS             652..1239
                     /locus_tag="PLU43_02815"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOO27370.1"
                     /translation="MKLLEDRIRRDGIVREGNILKVDSFLNHQMDIPLFCELGREFQK
                     RFATEHINKILTIEASGIGIACIVAQYFKVPVVFAKKSKTKNIAGDVYTSKVESFTHG
                     GIYDIIVSKQFLGAEDRVLIIDDFLANGKALEGLITLVDDAGATLVGAGIAIEKGFQT
                     GGRLIRSRGIHLESLAIVESMDAATGEIIFREDNG"
     gene            1336..2616
                     /locus_tag="PLU43_02820"
     CDS             1336..2616
                     /locus_tag="PLU43_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO27371.1"
                     /translation="MKKENTSFILKGNVCYSKSPKEAAFIKDACIVCSDGICGGVYKT
                     LPEKYKTLPLIDHRDALIIPGLTDLHLHAPQYPFRALGMDLELLEWLNTNTFPEEAKY
                     IDLEYAGKAYTMFVEDIKKGATTRACVFATIHAEATKLLMELLDKSGICAYVGKVNMD
                     RNSPEYLCEASAADSAQDTASWIEDTLHGYERIKPIITPRFVPTCSNELLERLGTLAK
                     NYHLPVQSHLSENPGEVAWVKELVPDAAFYGEVYDRYGLFGKEVPAIMAHCVYSDEKE
                     TERMLQNGVYIAHCPQSNTNIASGIAPIRRYLDMGMHIGLGTDVVDGYSTSILRAMAD
                     TIQVSKLRWRLCDHTLKPVSLEEAFYMGTKGGGSFFGKVGSFEEGYRFDAVVVEDSDL
                     VTPRLLTLRDRLERVVYMSENCKIKAKYVDGVQL"
     gene            2613..3056
                     /locus_tag="PLU43_02825"
     CDS             2613..3056
                     /locus_tag="PLU43_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HOO27372.1"
                     /translation="MKLIIDADACPVIAIAEKAAKEYRIPLLLICDTSHELTSAYGTV
                     MTVDKGADSADFRIIQTGSRGDLVITQDYGVASMALGKGMRVLHQSGMEYTNENIDRL
                     MFERHMAKKLRMGGKIRQKGPKKRTKEDDVKFAHSLYRILSETVY"
     gene            3111..>3516
                     /locus_tag="PLU43_02830"
     CDS             3111..>3516
                     /locus_tag="PLU43_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27373.1"
                     /translation="MGRVEKIIFGAVLSCLAVLAVPKAVKADPTYTIGMTGGQIYCTM
                     DSMDYTDISGTGVTQTADGIEIRLTDGSGNYATSWSTFTSSASGAYTYTMTANDSVDL
                     ITNSYLTQTYPATRTIDVRAVTTSGGSVTMYSA"
BASE COUNT          981 a          661 c          935 g          939 t
ORIGIN      
        1 cggaccgtgt ccttcgacag agccaagata taatgtaccg ccgattcctt cttttgcgtc
       61 ttttacctct tcggctgatt tgctgacaag tgccagtatt tttttagcat cctgtttaaa
      121 aattctgcct tccggtgtga gctgcagatg atggggcgtt ctgataaaaa gcagtgtgcc
      181 cagttctttt tccaggtcct gaatctggcg gcttagaggc ggctgtgcga tacaaagctg
      241 tttggcagct tttgtgaagt ttaagttttc cgcaactgca agaaaatatc ggatatgacg
      301 aagttccata aaaatctccc ttctatacaa aaaaagtatc atgatgtatt tattatatgt
      361 attttacgta taaaggtcaa cctgctatag taaaagtgta gaaaccagat cattttttaa
      421 tgaaaaggag gtttttcata tgagaagact atggagcata atagttgctt tttttgagga
      481 atattacagg gcatttgcca taccgttcga caggtaagaa aagttataat gagagcgctg
      541 caaaatggat gttttacatc tgttctgcag cgctctgtta ttgcaaacag ttcggttttg
      601 tagtatgatt taaatactat atgatacagc cggacgaagg gaaaatagca gatgaaatta
      661 ttagaagaca ggattcgcag agacggtatt gtaagagagg ggaatatact aaaggttgac
      721 agttttttaa atcatcagat ggatattccg ctgttttgcg aacttggacg tgagtttcag
      781 aaacgttttg ccacagagca tatcaataag attctaacga tcgaagcgtc aggaatcgga
      841 attgcctgta ttgtagcaca gtattttaag gttccggtcg tatttgcaaa aaagtccaaa
      901 acaaaaaata ttgcaggtga tgtttatact tccaaagtag aatcgtttac acacggaggg
      961 atttatgaca ttattgtttc caaacagttt cttggagctg aagacagagt actgatcatt
     1021 gacgactttc ttgccaacgg caaggcactg gaaggtctga taactctcgt tgatgacgca
     1081 ggagcgacgc ttgtcggagc cggcattgcg atcgagaaag gatttcagac gggcggaagg
     1141 ctgatccgtt caagaggaat tcatcttgag tcactggcaa ttgtagagag tatggacgct
     1201 gctaccggcg agattatttt tcgggaagat aacggttgag aaacgcacgg ggtttggata
     1261 ttgattacac aggggagggt taatttatat taactctttt tttcgacgtc tattgactgg
     1321 taaggagact ggctaatgaa aaaagaaaat acttcattta tactcaaagg gaatgtctgt
     1381 tacagcaaat ccccaaaaga ggctgcgttt attaaagatg cctgcatcgt ctgttcagac
     1441 ggcatctgcg ggggcgttta taagacgctt cctgaaaaat ataagacgct gccgctgatc
     1501 gatcatagag atgcgctgat cataccgggt ctgacggatc tgcatctgca tgcaccgcag
     1561 tatccgtttc gtgcactcgg catggatctg gagcttttgg aatggctgaa cacgaacacc
     1621 tttccggagg aagccaaata tatcgatctg gaatatgccg ggaaagcgta taccatgttt
     1681 gtggaggata taaaaaaagg agcaacgaca agggcctgtg tttttgcaac gatccatgct
     1741 gaggcgacaa agcttctgat ggaattgctt gataaaagcg gcatttgcgc gtatgtcgga
     1801 aaggtcaata tggatcgcaa ctcaccggaa tatctttgtg aagcaagtgc cgcagactcc
     1861 gcacaggata cggcttcgtg gatagaagac acgctgcatg gctatgaaag gatcaagccg
     1921 atcattacgc cgcggttcgt accgacctgt tcaaatgagc tgctggaacg gcttggcact
     1981 cttgcgaaaa attatcatct tccggtgcag tcacatttat cggaaaatcc cggagaggtt
     2041 gcatgggtaa aggagcttgt tccggatgcg gctttttacg gggaagtgta tgaccggtac
     2101 ggactgtttg gaaaagaggt gcctgcaatt atggcgcatt gcgtatattc tgatgagaaa
     2161 gaaacagagc gcatgctgca aaatggtgta tatattgcac attgtccgca gtccaataca
     2221 aatattgctt ccggtattgc gccgatccgc cggtatctgg atatggggat gcatatcgga
     2281 ctcggtacgg atgtggtaga cggttattcc acttcgatcc ttcgcgcaat ggcggatacg
     2341 atacaggtat cgaaactgcg ctggcgtcta tgtgaccata cgctaaaacc tgtcagtctg
     2401 gaagaggctt tttatatggg gacaaaaggg ggcggttctt tctttggaaa agtcggcagc
     2461 tttgaagagg gataccggtt tgatgccgtt gtcgtagagg atagcgatct tgtgactccg
     2521 cgtttgctga ctttgcgtga ccggctggaa cgtgtagtct atatgtcgga gaattgcaaa
     2581 ataaaggcca agtatgtgga tggtgttcaa ttatgaagct gattatagat gcggatgcct
     2641 gtcccgtaat tgccattgcg gaaaaagcgg caaaagagta ccggatcccg ctgcttctga
     2701 tctgcgatac gagccatgaa ctgacttctg cctatggaac ggtcatgaca gtagataaag
     2761 gtgcggattc cgcagatttc aggatcattc agacagggag caggggagac cttgttatta
     2821 cgcaggatta cggggtcgcg tccatggcac ttggaaaagg aatgcgtgtg cttcatcaga
     2881 gcggaatgga gtatacgaat gaaaatattg accgattgat gtttgaacgg cacatggcaa
     2941 agaagctgcg catgggcgga aaaatacgcc aaaaaggtcc gaagaaaaga acgaaggagg
     3001 acgacgtaaa atttgcgcac agtctgtatc ggatactttc ggaaactgtg tattagcagc
     3061 tgctttatgg aacaataatt gtatattagc cggtgggagg cgaaaagagc atgggaagag
     3121 tcgaaaaaat tatttttgga gctgtgttgt catgtttggc ggtactggcg gtgccgaaag
     3181 cggtaaaagc ggatccgacc tatacgatcg gcatgaccgg agggcagatc tactgtacga
     3241 tggacagcat ggattatacg gatatttccg gaactggggt tacgcagact gcagatggaa
     3301 ttgaaatcag acttaccgac ggttccggta attatgcgac ctcctggagt acgtttacaa
     3361 gcagcgcttc aggtgcatat acctatacga tgacggcaaa tgattccgtg gatctgatta
     3421 cgaacagcta tctgacacag acttatccgg caacgcggac gatcgatgta agggcagtga
     3481 cgacttccgg aggttcggtt actatgtatt cggcat
//
LOCUS       DAOSYE010000113         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18957_length_3510_cov_3.503907, whole genome shotgun sequence.
ACCESSION   DAOSYE010000113 DAOSYE010000000
VERSION     DAOSYE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18957_length_3510_cov_3.503907"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="PLU43_02835"
     CDS             <1..1898
                     /locus_tag="PLU43_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOO27374.1"
                     /translation="DYRYETDAEIVEPLDITGMAEAERTDLPLEKKRTTFLIYMNGSD
                     LESDGEGSASDDINEMQEALTVSDDINIILLTGGTLNWQRPEIPNGTYRIYELQKEGL
                     NFVCDMGSQNIGDPALLAGFINLGLTCFPAEKTGVILWNHGGGSIYGYGDDEITGDSL
                     LLTELNSAFASSEAAVKTLDFVGFDACLMGNLETACMLSPYADYMVASEEFEPGEGWD
                     YSFIGEFDQETEQGTPEECRNIVDSYVKGLNTKTDDWLDTQATLSVINLKTVGYLTDA
                     FDDFSEETSLHMLVGNYNEVARARDQVKSFGASGESDDCYDMVDIDSLAMEFQEISPE
                     KSTALTDALRETVVYSSHNANIDRACGLSVYFPYKSKSDVPEALETYEAMDILPAYTK
                     FMSRFSEKLLNESFDFSSFAAETPVQDEDGNFTAQLTEEELDSACNVYFTLWEQEEEL
                     EPGIYYYIELAETADLTVFDDGTVTTQFDGMWTTLNDNWVCMYEIDRTDTQIRYAIPA
                     YINEQDMDIIVVYDEQNPEGKVVGARPGSESETNMASRQMTEISPGDEITLYYWAELF
                     LDEGTDEELAENYEEGCWYSGEPWTVPDEGLVLARYELEEDEYLYSFALYDTANEVHY
                     TEFMEIE"
     gene            1967..3442
                     /locus_tag="PLU43_02840"
     CDS             1967..3442
                     /locus_tag="PLU43_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27375.1"
                     /translation="MNSTNKAVSEEQRINKMVVICYAILSFVFVASYIAEIFKGTYTF
                     AHTAVDFVFLLAPLVISLVLFFRNPDTVYIKDVLSVGYGLYYLVVMMTENDLSHYVYV
                     IPMLVLTSVFHNWKFTLRTGIGIVGILAAFSIKSLINGTVNAGDVEIALACVILSILF
                     VILASMSTERSYHARLELIRIEQQKEEENVAKLQEVIKGLLEKIDLLNASSKEMEGQS
                     IHSQSAIAEISTGTADVAKNIQKQLVMSNTINEKTEEANELSVELRDQFAVTKELSDG
                     GYDQLVEIDNTSVEFTKACDSVALTMQDLAEKIEQVRTTLDLINGVTTQTGLLSLNAS
                     IEAARAGESGRGFAVVAEQIKKLAEETKVATADIQTIFDALSAQSEKATETVHVLEEI
                     NTKQVGFVTKARNNFETIREDIVHASGQISAQAEHMREIRSSNAEISGNIENMSAFTE
                     ELFANAESTKALSEDTLKSVKKVNDILSDILSDVEKLKSII"
BASE COUNT         1036 a          607 c          954 g          913 t
ORIGIN      
        1 aggattaccg ctatgagact gatgccgaaa ttgtggagcc attagatatt acgggaatgg
       61 cagaggcgga gcgtacggat cttccgcttg aaaaaaagag aacgacattt ttgatttata
      121 tgaacggttc tgatctggaa agtgacggag agggcagtgc aagtgatgat atcaatgaga
      181 tgcaggaagc attgaccgtg tcggacgaca ttaatatcat actgctgacc ggcggtacgc
      241 tgaactggca gcgtcctgaa atcccaaatg gaacataccg gatctatgag ctgcagaagg
      301 agggactgaa tttcgtatgt gatatgggaa gccagaatat cggggatcct gcattgcttg
      361 ccggtttcat aaaccttggt cttacatgtt ttccagcaga aaaaacaggc gttatattat
      421 ggaatcacgg gggaggaagc atttatggtt atggggatga tgagatcacc ggagacagtc
      481 tgctgttgac agagctgaat tccgcgtttg catcaagtga agctgctgtc aaaacgcttg
      541 attttgtcgg atttgatgcc tgtctgatgg gaaatctgga aactgcgtgt atgctgagtc
      601 cctatgcgga ttacatggtt gcatcggagg aattcgagcc gggtgaaggc tgggattatt
      661 cttttatcgg ggaatttgat caggagaccg aacaggggac gccggaagag tgcagaaata
      721 tcgtggacag ctatgtgaaa ggactgaata caaaaacgga tgactggctt gatacacagg
      781 cgacgctttc tgtgatcaat ctgaagacgg tagggtatct gaccgatgca tttgatgatt
      841 tttcagaaga gaccagtctt catatgcttg ttggcaatta taatgaagtt gccagggcaa
      901 gagaccaggt aaaatcattc ggtgcgtcag gcgagtcgga tgactgctat gatatggtgg
      961 atatcgatag tctggcaatg gagtttcagg agatctcacc ggaaaaaagt acggcgctga
     1021 ccgatgcact gcgggaaact gtcgtatatt cgtctcataa tgccaatatt gaccgtgcat
     1081 gcggactttc cgtttatttt ccgtataaat ccaaaagtga tgtgccggag gcattggaga
     1141 cttatgaggc aatggatatt ctgcccgcat atacgaaatt tatgagccgg ttttcggaaa
     1201 agctgctgaa tgaatctttt gatttttcaa gctttgccgc cgaaacaccg gtacaggatg
     1261 aggatggtaa ttttaccgca cagctgacag aggaagaatt agatagcgca tgtaatgttt
     1321 attttacttt atgggagcag gaagaggaac tggaaccggg gatctattat tatatcgaac
     1381 tggcagagac ggcagatctt acggtatttg acgatggaac cgttacaaca cagtttgacg
     1441 gtatgtggac aacgctgaat gacaactggg tctgcatgta tgagatcgac cgcacggata
     1501 cgcagatacg gtatgcgatc ccggcgtata tcaatgagca ggatatggat attatcgttg
     1561 tatatgatga acagaatccg gagggaaaag tagtcggcgc ccgccctggc agcgaatccg
     1621 aaacaaatat ggcatccagg cagatgacgg aaatcagtcc gggcgatgag atcaccctgt
     1681 attactgggc ggaattgttt ctggatgaag gtaccgacga agagcttgcg gaaaattatg
     1741 aggaagggtg ctggtattcg ggagaaccat ggacggttcc tgatgaagga cttgtgcttg
     1801 ccagatatga actggaagaa gacgaatatc tatatagttt tgcactttac gatacggcaa
     1861 atgaggtgca ttatacggag tttatggaga tagaataatg ttggaaattt atgaacgttc
     1921 atgctagaat aaaagcatat gttatatata aaaaaggggg tacatcatga acagtactaa
     1981 taaggctgtc agcgaagagc agcggatcaa caaaatggtc gttatttgtt atgcgatcct
     2041 gtcatttgta tttgtagcgt cttatattgc ggaaatattt aagggaactt atacatttgc
     2101 gcatactgcg gtcgattttg tttttttgct cgcgccgctg gtaatcagtt tggttctttt
     2161 tttcagaaat ccggatacgg tatatataaa ggatgtactg tctgttggtt acggattata
     2221 ttatctggta gttatgatga cggaaaacga tctttcgcat tatgtctatg taattccaat
     2281 gcttgtactg acgtctgttt ttcataactg gaagttcaca ctgcggacag gtatcgggat
     2341 cgtcggaatc ttagcggctt tttcaatcaa aagtcttata aacggtacgg tgaatgccgg
     2401 agatgttgag attgcattag catgtgttat tttatccatt ttatttgtga tccttgcttc
     2461 gatgagtacg gaaagaagtt atcatgccag actggagctg atcaggatcg aacagcagaa
     2521 ggaagaggaa aatgttgcaa agctgcagga ggtgatcaag ggcctgctgg agaagataga
     2581 tttactgaat gcgtcgtcaa aggaaatgga aggacaatcg atccattctc agtcggctat
     2641 tgctgaaatc tcaacaggga cggccgatgt tgcgaaaaat attcagaaac agctggtaat
     2701 gagcaatacg atcaatgaaa aaacagaaga ggcgaatgaa ttatctgttg aactgcggga
     2761 tcaatttgct gttacaaaag aactgagcga cggaggatat gatcagttag tcgagatcga
     2821 taacacgtcg gttgaattta caaaagcctg tgattcggta gcacttacga tgcaggacct
     2881 ggctgaaaag atcgagcagg tccgtacgac gcttgatttg atcaacgggg tgacgaccca
     2941 gaccgggctg ctgagtctga atgcgagcat tgaagcggcc agagcagggg aatcaggacg
     3001 gggctttgcc gttgttgcgg aacagatcaa aaagcttgcg gaggaaacga aagtggcgac
     3061 agccgatatc cagacgattt ttgacgcgct ttccgcacag agcgaaaaag ctacggaaac
     3121 agttcatgtg ctggaagaga tcaacacaaa gcaggtggga ttcgttacaa aggcaagaaa
     3181 caattttgaa acgatcagag aagatattgt gcatgcgtcc ggccagattt ccgcacaggc
     3241 ggagcatatg cgggagatcc ggtcttccaa tgcggaaatc agcggaaata ttgaaaatat
     3301 gagtgcgttt acagaagaat tgtttgccaa tgcggaatca acgaaagcct taagtgagga
     3361 tacactgaag tccgtgaaaa aagtaaatga tattctttcc gatattttat cagatgtgga
     3421 gaaattaaaa tctattattt aagcggaacg attataaagt ttgtataaac cttcagactg
     3481 atgtaagata aacttacact ggatggaggt
//
LOCUS       DAOSYE010000114         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_18958_length_3510_cov_2.865123, whole genome shotgun sequence.
ACCESSION   DAOSYE010000114 DAOSYE010000000
VERSION     DAOSYE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18958_length_3510_cov_2.865123"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PLU43_02845"
     CDS             complement(<1..1323)
                     /locus_tag="PLU43_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOO27376.1"
                     /translation="MLKNITDWIKNVLFSRFFMLIIVFIVLFAILIQRLFVLQIINGE
                     DYLNNFTLTIKREKTLKSARGNIYDRNGQLLAYNELAYSVTIEDNYESGEDKDKKMND
                     TILSTVRLIEECGDKVLNNFNIVLNEYGKYEYTVSDTKLLRFLADVYGHASIDDLKTY
                     ERNATPDQVITYLAGENNYKIGEEETEGTKTVFVPKKGYSDEDVLQLITIRYAMSMND
                     YQKYLSTVIASDVNDETVAAIMENKDDLQGVDIEENTIRKYNDAKYFSHILGYTRNAS
                     SEDLENLADSDHDYTMTDMVGKAGVEKALEEELQGTKGKEIIYVDNVGKVIETTTHID
                     PKAGNDVYLSIDADLQKAIYNMLEQKLAGILVSKIINVKEYNNSEVKSSLMMIPIDDV
                     YFALINNNVININNFSEEDAQPYEAQVYSSFLSKQEEVLASLKEELLTT"
     gene            complement(1327..1833)
                     /gene="mreD"
                     /locus_tag="PLU43_02850"
     CDS             complement(1327..1833)
                     /gene="mreD"
                     /locus_tag="PLU43_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOO27377.1"
                     /translation="MLRKIIVFFMIIIGYLLQCTFFQNISLGGIVPNILIIITSSFGF
                     MRGKKEGMIIGFLAGLILDIFFGDILGFYSLIYMYIGYLNGFFRRIFYPEDIKLPMIL
                     ITLSELLYCFLCYIFLFLLRGKLHFGYYFIHVILPEIVYTILATLIVYKTILWVNEWL
                     ETKEKRSA"
     gene            complement(1859..2731)
                     /gene="mreC"
                     /locus_tag="PLU43_02855"
     CDS             complement(1859..2731)
                     /gene="mreC"
                     /locus_tag="PLU43_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOO27378.1"
                     /translation="MARNRSNRSNKNKFSIPSKYILLVLTIICIAMMILSFTTNVISG
                     PLETAAGYTIVPFQKGIAQVGLWLTDHSDNIKKMKELTAENEDLKTQIDELTIQINDL
                     QQDKYELAELRQLYLLDEQYSDYKKIGARVIGKDAGNWFSSFLIDKGTNDGLDVDMNV
                     MAGSGLVGIITEVGPNWATVKSIIDDTSNLSGMVLSSSDTLIVTGDLELMDEGYIRFS
                     QLIDKDDVVVQGDQIVTSHISNKYLPGISVGYITQINKDANNLTKSGYITPVVDFKHL
                     ETVLILTDLKQQKD"
     gene            complement(2744..>3510)
                     /locus_tag="PLU43_02860"
     CDS             complement(2744..>3510)
                     /locus_tag="PLU43_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO27379.1"
                     /translation="INDSMGGNIKPADYYISVPTDITEVEKRAFFDLVKDSNVRAREI
                     YIVEKAIADGIGLDVDVKNSQGILIVNIGFDTTEISILSLGGIVLSRLIKIGGKKFDE
                     SIRNIIRKEYNLLIGVKTAEKVKIALPELEEAEEDAVVYGRDIVTGLPVERSIPTILI
                     EKAMRENFEAIIDSIRVILERTPPELGADIYKKGIFLTGGSAGIGNLHELIGRSTGLK
                     VNFSPNGQESVAYGLSQVIGNEHFQSVAYSMQDFTN"
BASE COUNT          983 a          776 c          637 g         1114 t
ORIGIN      
        1 cgttgttaac agttcttcct tcagggaagc aaggacctct tcctgctttg aaagaaaact
       61 ggaatagacc tgtgcttcat agggctgtgc atcctcttcg ctgaagttat tgatattaat
      121 gacgttattg ttgatcaggg caaaataaac atcatcgatc ggaatcatca taagactgga
      181 ttttacttcg gaattattat attcctttac atttatgatt tttgaaacaa gaataccggc
      241 aagcttttgt tcaagcatat tataaatagc tttttgcagg tccgcatcaa tggaaagata
      301 cacatcgttt ccggctttcg gatcaatatg agtggtcgtt tcgatcacct tcccgacatt
      361 atccacataa atgatctctt ttccttttgt tccctgcagt tcctcctcca gagccttttc
      421 aacgccggcc ttgccgacca tgtcggtcat tgtataatca tgatcggaat ccgccagatt
      481 ttccaggtct tcagaagacg cattcctcgt ataaccaagt atatgagaaa aatattttgc
      541 atcattatat ttgcggatcg tattctcttc gatatcgacg ccctgcagat catctttatt
      601 ttccataatg gctgctaccg tttcgtcgtt gacatcggaa gcaatgacag tggaaaggta
      661 tttctgatag tcattcatgc tcatcgcata gcggatcgta ataagctgca gcacatcctc
      721 atcagagtac ccctttttag ggacgaacac tgtttttgtc ccttctgttt cttcttctcc
      781 gatcttgtag ttgttttccc ctgcgagata agtgatcacc tgatccggag ttgcgttgcg
      841 ttcataggtt tttaaatcat cgatgcttgc atggccgtaa acatccgcaa gaaaacgaag
      901 cagctttgta tcggaaaccg tatattcata ttttccatac tcattcagga cgatattgaa
      961 gttattcaga actttgtctc cgcactcttc gatcagacga accgtactta agatcgtatc
     1021 gttcatcttt ttatccttat cctcgccgga ttcataatta tcttctatcg ttacggaata
     1081 agccagctcg ttataggcaa gcagctgtcc gtttctgtca taaatatttc ctctggcgct
     1141 tttcagcgtt ttttctctct tgatagtaag tgtaaaattg ttcagataat cctcgccgtt
     1201 aatgatttgt aaaacaaata atcgttgtat taaaattgca aacagcacga taaatacaat
     1261 aatcagcatg aaaaatctgg agaacaatac attttttatc cagtccgtaa tatttttcag
     1321 caaagatcat gcactccttt tttcttttgt ttcaagccat tcgtttaccc ataggatggt
     1381 cttgtaaacg atcagtgttg caaggatggt ataaacgatt tcaggcagaa tgacatgaat
     1441 gaaatagtaa ccaaaatgca gttttcctct taataaaaat aagaatatgt aacaaagaaa
     1501 acaatacaat aattcactta atgtgatcag gatcatcggc agtttgatat cttcaggata
     1561 aaaaattctt ctgaaaaatc cgttcagata gccgatatac atgtaaataa gtgaataaaa
     1621 tccaagaata tcaccaaaaa aaatatccag gatcagaccg gccagaaacc cgatgatcat
     1681 tccttctttt ttaccgcgca taaaaccaaa ggaggaggta atgatgatca atatatttgg
     1741 aacgatcccg ccgagtgaaa tattctgaaa aaatgtacac tgcagcagat atccaataat
     1801 aatcataaaa aaaacaatta tttttctaag catatcatga tttcactctc tttcagattc
     1861 agtccttttg ctgctttaag tctgtaagga tcagcacggt ctccaaatgt ttaaaatcta
     1921 caacaggggt aatatatccc gattttgtca aattgtttgc atccttattg atctgcgtga
     1981 tatagccgac cgaaataccg ggcagatact tgttactgat atgggaggtg acgatctgat
     2041 ccccctgtac gactacgtca tccttgtcga tcagctgtga aaaacggata tagccttcgt
     2101 ccatcagttc cagatctccg gtaacgatca gcgtgtcgga agaagaaagt accataccgc
     2161 ttaagttgga ggtatcgtca ataatggact tgacggtcgc ccaatttggt ccgacttctg
     2221 ttataatgcc gacaagaccg cttccggcca taacattcat atcaacgtca aggccatcat
     2281 ttgttccttt atcaatcaaa aaagaactga accagttccc tgcgtctttt ccgatcaccc
     2341 gtgctccgat ctttttatag tcagaatact gttcatcgag taaatacagt tgtctaagct
     2401 ccgcaagttc atatttatcc tgttgcaggt cattgatctg gatcgtcagc tcatcgatct
     2461 gcgtcttcag atcttcattt tccgcagtca gctctttcat ctttttgata ttatccgaat
     2521 gatctgtcag ccataaccct acttgtgcaa ttcctttctg aaaaggaacg atcgtataac
     2581 cggcagcggt ttcaagcgga ccgctgatga catttgtcgt aaatgataaa atcatcatgg
     2641 cgatgcagat gatcgtcagt accagtaaaa tatatttact tggaatagaa aatttattct
     2701 tattggatct gttggatcga ttccttgcca tttccgggtc cccttaattt gtaaaatcct
     2761 gcatggaata agcgacagac tggaaatgtt catttccgat tacctgggat aatccataag
     2821 cgacactttc ctggccattt ggtgaaaaat ttactttcag ccccgtactt ctgccgatca
     2881 gttcgtgcag gttgccgatg ccggccgaac cgcctgttaa aaagattcct tttttataaa
     2941 tatccgcacc cagttccggc ggtgttcgtt ccagaataac gcggatgctg tcaataattg
     3001 cttcaaaatt ttctctcatc gctttttcaa ttaatatggt tggaatactt ctttcgactg
     3061 gaagacccgt gacaatatct cttccataga cgactgcgtc ctcttccgcc tcttccagtt
     3121 caggaagcgc gatttttact ttttctgcgg tttttacacc aatcagaagg ttgtattctt
     3181 ttctgataat atttcgaatg ctttcatcaa attttttacc gcctattttg atcagacggg
     3241 aaagaacgat tccgccaagc gacaaaatgg aaatttccgt cgtatcaaaa ccaatgttga
     3301 cgatcagaat accctgtgaa tttttcacat ccacatccag tccgatcccg tctgcaattg
     3361 ctttttcaac aatatagatt tcccgggccc tgacattgga atctttaaca aggtcaaaaa
     3421 acgccctctt ttccacctcc gtgatatcgg tcggtacgga aatatagtaa tctgccggct
     3481 tgatattacc gcccatggag tcgttgatga
//
LOCUS       DAOSYE010000115         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19009_length_3505_cov_2.994493, whole genome shotgun sequence.
ACCESSION   DAOSYE010000115 DAOSYE010000000
VERSION     DAOSYE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19009_length_3505_cov_2.994493"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLU43_02865"
     CDS             <1..1118
                     /locus_tag="PLU43_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO27380.1"
                     /translation="GNLLMPGFKNAHTHSGMTLMRSYADGLPLQEWLEQKIFPLEAKM
                     TGEDIALLTKLAILEYLTSGVTSIFDMYLSPEAIAKACEEAGMRVVQVSGINKFGPSV
                     EVLEERYLKLNNRHPLSSFMLGFHAEYTCTKELLEKIAAMAHKYEAPVYTHNSETVTE
                     VSGCMERYGMTPTVFLDSLGMFDYGGGGYHCVHMTEEDIEIFKKRRMSIVTNPGSNTK
                     LASGIAPIQRFLDEGINVAIGTDGPSSNNCLDMFREMFLVTGLAKLREKDAAAADAMD
                     VLKMATTGGAKAMGLKDCDVLAEGKEADMIMIDLHQPNMQPFNDIERNIVYSGSKQNI
                     KLTMIHGKILYEDGKFNIGMDPEDIYKEANNILKRIG"
     gene            1154..1873
                     /locus_tag="PLU43_02870"
     CDS             1154..1873
                     /locus_tag="PLU43_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO27381.1"
                     /translation="MKVTYVYHSCFIVELEKSILIFDYFKGQLPQLSPTKHIYLFASH
                     KHQDHFSMEIFGLFKAPFQVTYILANDIRLSERYLERLGFESSIKKQIVSAGKCMEIS
                     LHEDFTIKTLKSTDAGVAFIIETEGVCLYHAGDLNWWHWKEESDVFNLYQERVYAQQL
                     EKIRGQYFDAAFLPLDYRLGDAKEWGLVKFLEMTEAETVFPMHMWEHYELIDECLANG
                     RLAVYKDRIVRISAENESWTL"
     gene            1873..>3505
                     /locus_tag="PLU43_02875"
     CDS             1873..>3505
                     /locus_tag="PLU43_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27382.1"
                     /translation="MEWVLFFIAVIILIAVMFMNGISRERKAYRAYCDSLREDFGKVP
                     ALDYSETDMDRIAEYHNKMLELNRVSGFALDDITWNDLDMGQIFMQMNVTQSSIGEEY
                     LYHVLRCPYLQKEGLEKWDSLVDFFSENETGRVEVQKLLHKFGKIQKFSLTKIIYTMM
                     ELQKESNLIHIILDVLLAASFCMIFVLPGPGVLFFITMLAVSIITYFRRKNEVSPYFT
                     TFLYILNMLRAAEAINKLQYDVLLEEQERLHAVQKAFDGFEKNTYVLSTSTHTTDNPV
                     ELLLDYIRMLFHADIIKFNSMLQTVQNHINEIEELREILGRLDAAAAAASFKKSLAYA
                     CRPQFVKKELSKRAVLEINEAFHPLVEKPVANSIHAKDGVLITGSNASGKSTFLKTIA
                     ICAILAQTIGIVPAKSYRGPLFRIYSSMALKDNIRTKESYYIVEIKSLKRIVDASAIE
                     NPLLCLIDEVLRGTNTVERIAASSQILKGLLKPYVLCFAATHDIELTHILETCYTNYH
                     FDEEVLENDIIFSYHLHKGRAETRYAIKLLSIIGYE"
BASE COUNT         1058 a          616 c          872 g          959 t
ORIGIN      
        1 aaggaaacct gctgatgcct ggttttaaaa acgcacatac acattcagga atgacactga
       61 tgcgttctta tgcagacggc cttccgctgc aggaatggct cgagcagaaa atttttccgc
      121 tggaagccaa aatgacaggg gaagatattg cgctgcttac aaaattggcg atacttgaat
      181 atctgacaag cggtgtaaca tcgatctttg atatgtatct gtctccggag gccattgcga
      241 aagcatgtga agaggcaggc atgagagttg ttcaggtgag cgggatcaat aaattcggcc
      301 cttctgtgga agtgctggaa gaaaggtatc tgaagctgaa caacagacat ccgctttcca
      361 gttttatgct tggatttcat gcggaatata catgtacaaa ggaattgctt gaaaaaatag
      421 cagccatggc tcataaatat gaggctccgg tctatacaca taattcggaa accgtgactg
      481 aagtcagcgg atgcatggaa cgttatggaa tgactccgac ggtattcctt gattctcttg
      541 ggatgtttga ttacggcgga ggcggatatc actgcgtaca tatgacggag gaggatatcg
      601 aaatatttaa aaagcgccgg atgtccattg taacaaatcc tggttcaaat acaaagctgg
      661 ccagcgggat cgcgccgata cagagatttc ttgatgaggg aatcaatgtt gcaatcggca
      721 cggacggacc gtccagcaac aactgtctgg atatgtttcg tgagatgttc cttgtaacag
      781 gacttgccaa actcagagaa aaagacgcgg cagcagcaga tgccatggat gtgttgaaaa
      841 tggcgactac gggcggagca aaggcgatgg gactcaaaga ctgcgatgtg cttgccgagg
      901 gtaaggaagc ggatatgatc atgatagatc tgcatcagcc caatatgcag ccctttaatg
      961 atattgaaag aaatattgta tacagcggca gcaagcagaa tatcaaactg accatgatac
     1021 atggaaaaat tctttatgaa gacggaaaat ttaatatcgg catggatcct gaagatattt
     1081 ataaagaagc aaacaatatt ttgaaacgga tcggctgaca cttaagagag caaaaaggaa
     1141 gcaacagaca ataatgaaag ttacgtatgt gtatcacagc tgttttattg tagagcttga
     1201 aaagagcatt ttgatatttg actattttaa ggggcagctt ccgcagcttt ccccgacaaa
     1261 gcatatttat ctgtttgcaa gccataagca tcaggaccat ttttccatgg agatctttgg
     1321 tttgtttaag gctccgtttc aggtaacata tatacttgca aatgatatca ggttaagtga
     1381 acggtatttg gagagattgg ggtttgaatc atcgataaag aaacagatcg tctctgcggg
     1441 aaagtgtatg gagatttccc ttcatgagga ttttacgata aaaacgctga aatctacgga
     1501 tgcaggggtg gcgtttatta ttgaaacgga aggtgtgtgc ctgtatcatg ccggagatct
     1561 gaactggtgg cactggaaag aggaaagcga tgttttcaat ctctatcagg aacgtgttta
     1621 tgcacagcag ctcgaaaaga taaggggaca atattttgat gctgcttttt taccattgga
     1681 ttacaggctt ggtgatgcaa aagaatgggg gctcgttaaa tttttagaga tgacggaggc
     1741 agaaactgtt tttccgatgc acatgtggga gcactatgaa ctgatcgatg aatgtcttgc
     1801 caatgggaga ctggcggtct ataaagaccg gattgtccgg atcagtgcag aaaatgaaag
     1861 ttggacgtta taatggaatg ggttttattt tttattgcag tcatcatatt gatcgcagtg
     1921 atgtttatga atggcatatc gcgggaaaga aaggcatatc gggcttactg cgattcgctc
     1981 agggaagatt ttgggaaagt tccggctctt gattattcgg agacggatat ggacaggatc
     2041 gctgagtatc ataataaaat gctggagctc aacagggttt ccggatttgc cctggatgat
     2101 atcacatgga acgatttgga tatgggacaa atttttatgc agatgaatgt aacacagtcc
     2161 tcgatcggag aagaatatct ctatcatgta ttgcgctgtc cttatcttca gaaggaaggt
     2221 ctggaaaaat gggacagtct tgtggatttt ttttcggaaa atgaaaccgg acgcgtggaa
     2281 gtacagaaat tactgcataa attcgggaaa atacaaaagt tttccctgac aaaaattatt
     2341 tatacaatga tggagcttca aaaggagagc aatctgatcc acattatttt agatgttctg
     2401 cttgctgcga gcttctgcat gatttttgtg ctgccgggac cgggtgttct ttttttcata
     2461 acaatgcttg cggtttcgat catcacctat tttagaagaa aaaacgaagt gtcgccttat
     2521 tttacaacct ttctttatat tttgaacatg ctgagggcgg cagaagcaat caataaactg
     2581 cagtatgatg tgcttttgga agagcaggaa cggctgcacg cggtacagaa ggcttttgac
     2641 ggttttgaaa aaaatacgta tgtattatcg acgagcaccc atacgactga taatccggtc
     2701 gaactgctgc tggattatat cagaatgtta tttcatgcgg acattataaa attcaactcc
     2761 atgctgcaga ctgtgcagaa ccatataaat gaaattgagg aattaagaga aatactcggg
     2821 agactggatg cggcggcagc ggcggcgtct tttaaaaaga gcctggcgta tgcatgcaga
     2881 cctcagtttg ttaaaaaaga gctttccaaa agagctgtgc tggaaatcaa tgaggcgttt
     2941 catccgcttg tggagaagcc ggttgccaac agtatccatg caaaggacgg cgttttgatc
     3001 acaggttcga atgcatccgg aaaatccaca tttttaaaga caatagcgat ctgcgcaatt
     3061 cttgcacaga cgatcggtat tgtaccggct aaaagttaca ggggaccgtt gtttcgcatt
     3121 tattcttcaa tggcgctgaa agataatatt cggacaaagg aaagttatta tattgtggaa
     3181 attaaatcgt tgaaacggat cgttgatgcc tcagcaatag agaatccgct tttatgcctg
     3241 atcgatgaag ttttgcgcgg gacgaatacg gtagagcgga tcgcagcctc atcacagata
     3301 ttaaaagggc ttctgaaacc gtatgtcctc tgttttgccg caactcatga catagagctt
     3361 actcatatac tggagacttg ttatacaaat tatcattttg atgaggaagt gcttgaaaat
     3421 gatattattt tcagttatca tctgcataaa ggaagagcgg aaacaagata tgccattaaa
     3481 ctgctttcga ttataggata tgaac
//
LOCUS       DAOSYE010000116         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19044_length_3501_cov_3.562101, whole genome shotgun sequence.
ACCESSION   DAOSYE010000116 DAOSYE010000000
VERSION     DAOSYE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19044_length_3501_cov_3.562101"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLU43_02880"
     CDS             complement(<1..309)
                     /locus_tag="PLU43_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO27383.1"
                     /translation="MLTKRNDIRNIAIIAHVDHGKTTLVDELLKQSGVFRENQEIVER
                     IMDSNDIERERGITILSKNTAVTYKDTKINIIDTPGHADFGGEVERVLKMVNGAILVV
                     D"
     gene            537..1022
                     /locus_tag="PLU43_02885"
     CDS             537..1022
                     /locus_tag="PLU43_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO27384.1"
                     /translation="MSEGLIYIYCGDGKGKTTAAVGQAIRIASQSGTVFIVQFLKGKN
                     PTELDFIRRLEPEIKLFTFEKNDEFYLELSAEEKKEAAANIRNGLNFAKKVLTTGECD
                     CLVLDEALGLIDNEIATIADFQTLIKAKPENASLIMTGIHLPDEICMMADEVSRVVAV
                     K"
     regulatory      1101..1286
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1411..2307
                     /gene="dapA"
                     /locus_tag="PLU43_02890"
     CDS             1411..2307
                     /gene="dapA"
                     /locus_tag="PLU43_02890"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO27385.1"
                     /translation="MAVFTGAGVAIVTPFKDNGEVNYEKFAEQIEYQIANKTDAIIVC
                     GTTGEASCLSHEEHLACIKFCVDTVKKRIPVIAGTGSNCTETAVYLSTEAEKYGVDGI
                     LVVTPYYNKATQNGLIEHFTAVADSVSVPMILYNVPSRTGVNILPETAVTLCRTVKNI
                     VGIKEASGNIGQVARLMSLADGCVDLYSGNDDQIVPILSLGGKGVISVLSNIAPEQTH
                     DIVQTYLDGDAAKSCELQLKAIPLCDALFCEVNPIPVKKAMNLQARGAGVMRRPLTEM
                     EPQNAAKLEKVMKDYGVLETRV"
     gene            2310..3065
                     /gene="dapB"
                     /locus_tag="PLU43_02895"
     CDS             2310..3065
                     /gene="dapB"
                     /locus_tag="PLU43_02895"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOO27386.1"
                     /translation="MVNIIMHGCNGRMGQMITNIAAEDPQICITAGVDINNVQKNEYP
                     VFQTISDCTVEADAVVDFGAAAAVDGLLNWCAEKQVPVVVCTTGLSEEQLAHLEAVSK
                     QVAVLRSANMSLGINMLMKLLKEAANTLAGAGFDIEIVEKHHKMKLDAPSGTALALAD
                     SINEARDHEYTYVYDRSQVRQKRGEKELGISAVRGGSIVGEHEVIFAGQDEVVTFTHT
                     AYSRAIFGKGAVQAAKFLKGKPAGLYDMSDVIG"
     gene            3265..>3501
                     /locus_tag="PLU43_02900"
     CDS             3265..>3501
                     /locus_tag="PLU43_02900"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOO27387.1"
                     /translation="MNKPEKSYLKILAKQYPTIAAASTEIINLQSILNLPKSTEHFMT
                     DIHGEYEQFVHVLKNGSGSVKRKIDEEFGNTLSVK"
BASE COUNT          989 a          658 c          958 g          896 t
ORIGIN      
        1 atccacaaca aggatcgcac cgtttaccat tttcaggacg cgttctactt ccccgccgaa
       61 atcggcatgt cccggagtat caataatgtt tattttagta tctttataag tgaccgccgt
      121 gtttttggac agtatcgtaa tgccccgttc ccgttcgatg tcgttggaat ccatgatccg
      181 ttcgacgatc tcctgattct cacgaaatac gccgctctgt ttcagcagct catcgacaag
      241 tgttgttttg ccgtgatcga catgggcgat gatggcgata tttcggatat cgtttctttt
      301 ggttaacata aaattttcct tcctgtatct gtatctgtaa aaactctgta aatgacggca
      361 ctgacagtgc ctaaccgtaa tagtatagca tattttatca ggaaaacaag aagaatcaaa
      421 ataaaagttg attttgcgga acattgatga tatgatatgc atcgaatatt tctattattt
      481 tgatacatga ggtgtggttt tggaaaggac tgtttccggt ccggttgagg tgacttatga
      541 gtgagggttt gatttatatt tattgcggag acggaaaagg aaagacaact gctgcggtag
      601 ggcaggcgat ccgtattgcc agtcaaagcg gaactgtttt tattgttcag tttctgaaag
      661 ggaaaaatcc gactgagctt gattttatca gacgtctgga gccggagatc aagctgttta
      721 cgtttgaaaa aaacgatgag ttttatctgg agctgtccgc tgaggagaaa aaggaagcgg
      781 ccgcaaatat tcggaacggg ttaaattttg caaaaaaggt gcttactacc ggcgaatgcg
      841 actgtcttgt acttgatgaa gcgctgggcc tgatcgataa tgagatcgcg accattgcag
      901 attttcagac gctgatcaag gcaaaaccgg aaaatgcaag cctgatcatg acagggatcc
      961 accttccgga tgagatctgt atgatggctg atgaagtttc cagggtcgtt gctgtcaagt
     1021 agaaatccgg atgtctgaat cgttcgccgg gtcttgacaa cgttacagag ggatgctatg
     1081 atgcatataa caattttata acagaggata gaggttgcgc tttttatcag tacattttcg
     1141 tacattactc agatggagaa agaagatgga aaggaaacgg cgccgaaaaa gcagatgtga
     1201 gaagtctgtt tttgggcatg catttaacag gtgcatgact gtcatctttc aacagcattg
     1261 ttgtgggatg gggagctatc aaaagagaca atttcatgtg gattgttgat tttaagccag
     1321 cccatctcgg ttggctttta ttaatgcttt cacatttaac aaagccggct gagtttttat
     1381 caatcgaacc atattaagga ggaaaagagt atggcagttt ttaccggagc gggcgttgcg
     1441 atcgtaacac cgtttaaaga caacggagag gtgaattatg aaaagtttgc ggagcagatc
     1501 gaataccaga tcgcaaacaa aacagatgcg atcattgtat gcggtacaac aggggaagcg
     1561 tcctgtttaa gtcacgagga gcaccttgcg tgtattaaat tctgtgtgga cacggtcaaa
     1621 aaaagaatcc cggtaattgc gggaacaggc tccaattgta cggagacagc cgtctattta
     1681 tctacggaag cggaaaagta tggtgttgac ggaattcttg tcgtaacgcc ttactataac
     1741 aaggcaacac agaacggact gatcgagcat tttacagcag ttgcggacag tgtatcggtt
     1801 ccgatgattt tatacaatgt gccgagcagg accggtgtga atattctgcc ggaaacggcc
     1861 gtaactcttt gcagaacggt caaaaatatt gtcgggatca aggaagcgtc cggaaatatc
     1921 gggcaggttg caagattaat gagtctggcg gacggatgtg tggatctgta ttccggcaac
     1981 gacgaccaga tcgtgccgat cctgtcactt ggcggaaagg gagtaatttc cgtattgtca
     2041 aatattgcgc cggaacagac acatgatatc gtgcagacgt atctggatgg tgatgcggca
     2101 aagagctgtg agctgcagct gaaggcgatt cctctttgcg atgcgctgtt ttgcgaagtc
     2161 aatccgatcc cggtcaagaa agcgatgaat ctgcaggcaa gaggtgccgg agtcatgcgc
     2221 cggccgctta cggaaatgga gccgcagaat gcggcaaagc tggaaaaggt catgaaggat
     2281 tatggagttt tggaaacgag ggtttaataa tggtaaatat aattatgcac ggctgtaacg
     2341 gccgtatggg acagatgatc acaaatatcg cagcagaaga tccgcagata tgtattacgg
     2401 ccggggttga tatcaacaac gtacagaaaa atgagtatcc ggtgtttcag acgattagcg
     2461 actgcacagt agaagcagat gcggttgtgg attttggtgc ggctgctgca gttgacggac
     2521 ttcttaactg gtgtgcggaa aagcaggtgc cggtcgttgt ctgtacgaca ggactgagtg
     2581 aggagcagct tgcacatttg gaggcggtca gcaagcaggt agcggtgctg cgttccgcca
     2641 acatgtccct tggcatcaac atgctgatga agcttttgaa ggaggcggcc aatacgcttg
     2701 ccggggcagg ctttgatatt gagatcgtgg aaaagcatca taagatgaag ctggatgcgc
     2761 cgagcggtac ggcgctggca ctggctgatt cgatcaatga agccagagat catgaatata
     2821 cgtatgtata cgacaggagt caggtcagac agaaacgcgg ggagaaggag ctcggtattt
     2881 cagcggtccg cggcggaagc attgtcggcg agcatgaggt cattttcgcc ggacaggacg
     2941 aagttgtgac gtttacgcat acggcgtatt caagagccat attcgggaaa ggtgccgtgc
     3001 aggcagccaa atttttaaaa ggaaagccgg ccggacttta tgatatgagc gatgtgatcg
     3061 gatagcggaa aatgagataa aaatatgaga aaaggactgc tgtaaaataa ccggagagcc
     3121 ggctgttttg cggcggtcct tttctgccgg gataaatttc cggctgcgtg cgctgcaata
     3181 tttgtatgaa aatcacttgt gtccgtttca tatcaagtgt aaaatgaaac cagacacagg
     3241 aaatataatt gggggtatcg agtaatgaat aaaccggaaa aaagttattt aaaaattctt
     3301 gcaaagcagt atccgacgat cgcggcggca tcgacggaaa tcatcaatct gcagtctatt
     3361 ttgaacctgc caaagagcac ggaacatttt atgacggata ttcatgggga atatgaacag
     3421 ttcgttcatg tgctgaagaa cggttccggt tcggtaaagc gaaagatcga cgaggagttt
     3481 ggaaacacac tgagcgtaaa g
//
LOCUS       DAOSYE010000117         3499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19062_length_3499_cov_3.365273, whole genome shotgun sequence.
ACCESSION   DAOSYE010000117 DAOSYE010000000
VERSION     DAOSYE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19062_length_3499_cov_3.365273"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLU43_02905"
     CDS             <1..231
                     /locus_tag="PLU43_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOO27388.1"
                     /translation="ADNRKIVGDADILLFPDIHAGNLVYKAIVRLANVKNGCILTGTK
                     APVILTSRSDTLETKVNSIALAAIVAEELSKA"
     gene            246..1313
                     /gene="buk"
                     /locus_tag="PLU43_02910"
     CDS             246..1313
                     /gene="buk"
                     /locus_tag="PLU43_02910"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOO27389.1"
                     /translation="MSIKSLIINPGSTSTKIGVFEDETLLFEETLRHSTEEIAKYGSI
                     VEQKDFRKEIILNLLKEKNFDINSLQVIVGRGGMLKPIPGGTYAVTDSLLNDLKIGVQ
                     GQHASNLGGILAKEIGDSIGVPSYIVDPVVVDELMPIARYSGVPELPRVSIFHALNQK
                     AVAKRYAKEIGKAYDSLNLIVVHMGGGVSVGPHKNGKVIDIFNALDGDGAFSPERAGG
                     VPSGALIKMCFSGKYSEKEVYKKICGNGGFNAYLQTNDMRDVEKMVDDGDAKAREIRD
                     AFILQVAKDIGSMAAVLDGKVDQIIATGGIAYDKGVIAGLKARCEWIAPFTVYPGEDE
                     LLALVQGALRVMNGEEKAMEY"
     gene            1332..2360
                     /locus_tag="PLU43_02915"
     CDS             1332..2360
                     /locus_tag="PLU43_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO27390.1"
                     /translation="MNCSRKYGKPFGDLNKLKTNFFENNDGMLEMADHMAKIFSAQPR
                     RTNCKICGEPLGEPLFHSHGIGYIECSKCGHLNGEFEDTDDFASKVYIQDDYSKNYSS
                     KDREEYNRRLSTIYIPKAEYLQSCMEKEQLADIRLLDIGAGCGYFVAAAKKLGMQASG
                     IEISENQVNYGNTMADETILTHVGLHDSINFIEKTDANVISAIGVLEHLINLQENLDA
                     IGSNHNIQYLYASVPMFSFSCAFEAANQQCYNRHTGGTHTHLFSNSSIRYMAEQMGFE
                     PVYEWRFGSDINDLYRFLTMMLTKSGNSAFASDFSGKFVPLMDELQLILDKSDFSSEL
                     HFILKRKQ"
     gene            complement(2362..2766)
                     /locus_tag="PLU43_02920"
     CDS             complement(2362..2766)
                     /locus_tag="PLU43_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27391.1"
                     /translation="MEKEAGCFKVWTDQNDRRIIYSKGHGIARAHEIEWLCDTISEFS
                     KEWQDDKGWAYVALIEELHQVSMKGSSRYVKLHETLAADKCKYVVYIEGNSYEVSVQS
                     SKHKQMSNTPETENKYFLTMEEGLLWLKENGF"
     gene            complement(2949..>3499)
                     /locus_tag="PLU43_02925"
     CDS             complement(2949..>3499)
                     /locus_tag="PLU43_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO27392.1"
                     /translation="MIKVIDAAVAKAYGGEKIDPIKGAKAVVEECKVEFVPNKKYVTV
                     KEVVKVVSDTVDIMDAKILISGGRGVGSPENFKILEDLAKAIGGTVSCSRAVVDSGWK
                     PKDLQVGQTGKTVRPNVYFAIGISGAIQHVAGMEEADIIVAINKDEDAPIFDVADYGV
                     VGDLNTIVPQLTEKIKAVMADK"
BASE COUNT         1016 a          804 c          727 g          952 t
ORIGIN      
        1 gccgacaacc gtaagatcgt cggagatgcc gacatcctgc tgtttcccga tatccatgcc
       61 ggcaaccttg tatataaggc aatcgtccgt ctggcaaatg taaagaacgg atgtatttta
      121 acaggcacga aagcaccggt catcctgact tcccgttccg atacgcttga aacaaaagta
      181 aattcgatcg cactggcagc cattgttgcc gaagaattat caaaagcata gaaggagaat
      241 gtatgatgtc aattaaaagt ttaattatca acccgggctc aacttctaca aaaatcggcg
      301 tatttgaaga cgaaacgctc ttattcgaag aaacactcag acactctacg gaggagatcg
      361 caaaatacgg ttcgatcgta gaacagaagg atttcaggaa agaaattatc ctcaatctgt
      421 taaaagaaaa aaatttcgat ataaattcac tgcaggtgat cgtcggccgc ggcggcatgt
      481 taaaaccgat cccaggcggt acatatgcgg taactgattc cctgcttaat gatctgaaaa
      541 tcggcgtaca ggggcagcat gcttctaatc tcggcggaat tctcgcaaaa gagatcggag
      601 attccatcgg cgttccttcc tatatcgtcg atcctgtcgt tgttgacgaa ctgatgccca
      661 ttgcaagata ttccggcgta cccgagctcc cgcgtgtcag tattttccac gccctgaatc
      721 agaaagccgt cgcaaaacgc tatgcaaagg aaatcggcaa agcctatgat tccctgaacc
      781 tgatcgtcgt acatatgggc ggcggtgttt ccgtaggtcc tcacaaaaac ggaaaggtta
      841 tcgatatttt taacgcgctt gacggcgacg gtgccttttc accggaaaga gcaggcggtg
      901 ttccttccgg cgcactgatc aaaatgtgct tttccggcaa atattcggaa aaggaagtct
      961 ataaaaagat ctgcggaaac ggcggcttca atgcttatct gcagacaaac gacatgcgtg
     1021 acgtagaaaa aatggtcgat gatggtgatg caaaagcaag ggagatccgc gatgcattta
     1081 ttctgcaggt cgcaaaagat atcggttcca tggctgctgt tctggacggc aaggtggatc
     1141 agatcattgc aaccggtggt attgcgtatg ataagggagt gattgccgga ctaaaagcca
     1201 gatgtgaatg gatcgccccg tttaccgtat atccgggtga agatgaactg cttgcacttg
     1261 tgcagggcgc tctgcgtgtt atgaacggcg aagaaaaagc aatggaatac taaacataaa
     1321 gggagaatac tatgaattgt tccagaaaat atggaaaacc tttcggtgac ctgaataaat
     1381 taaaaacgaa ttttttcgaa aataacgatg ggatgctgga aatggccgat catatggcaa
     1441 aaatattttc tgcccagcca agacgtacca actgtaagat ctgcggcgaa cctttaggcg
     1501 aaccactgtt tcacagtcac gggatcggct atatcgaatg ttccaaatgc ggccatttaa
     1561 acggtgaatt tgaagatacg gatgattttg caagtaaagt ctatatccag gatgactatt
     1621 ccaaaaacta cagttccaaa gacagggaag aatacaaccg gcgtctttcc acaatttata
     1681 ttcccaaagc agaatatctg cagtcctgta tggaaaaaga acagctggcc gatatcagat
     1741 tactggatat tggtgcaggc tgcggttatt ttgtagctgc tgccaaaaag cttggcatgc
     1801 aggcaagcgg tatcgaaatt tctgagaatc aggtcaacta cggaaataca atggcagatg
     1861 aaacaatact gacacacgtc ggtctgcatg attcgataaa ttttatcgaa aaaacagacg
     1921 ccaatgtcat ctcagccatc ggtgttctcg aacacctgat caatctgcag gaaaacctgg
     1981 atgccattgg cagtaatcac aatattcaat atttatatgc ctctgttcca atgttctctt
     2041 tcagctgtgc attcgaagca gcaaatcagc agtgctataa ccgtcatacc ggcggcacac
     2101 atacacattt attttccaac agttctattc ggtacatggc agagcaaatg ggctttgagc
     2161 cggtatatga atggagattt ggttcggaca tcaatgatct ctaccgattt ttaacaatga
     2221 tgcttacaaa atccgggaac agcgcatttg cttccgattt ttccggaaaa tttgtaccat
     2281 taatggatga attacagctg attctggata aaagcgactt ttcttcggaa ctgcatttca
     2341 ttttaaaaag aaaacagtag atcagaaacc attttcttta agccataaaa ggccttcttc
     2401 catggtcaga aaatatttat tttcagtttc aggtgtattg gacatctgct tatgctttga
     2461 agactgtacg gatacctcat aggaatttcc ttctatgtaa acaacatatt tacatttgtc
     2521 ggcggcaagt gtttcatgca gtttcacata gcgggaactt cctttcatac ttacctgatg
     2581 tagttcctct ataagcgcaa catatgccca tcctttatcg tcctgccatt cttttgaaaa
     2641 ttccgagatc gtatcacaaa gccactcgat ttcatgggct cttgcaatac catgtccttt
     2701 tgaataaata attctccgat cattctgatc agtccagact ttaaaacatc ctgcctcttt
     2761 ttccattcca ttgcctcctt catacacttt catcataaaa atgactaaag taaattgtat
     2821 cggatattgt ggaaaatttc aagggatttt cttacgtaat tctgttcttc cttaagaaca
     2881 aaaaaggaag tatgacaggc atggagcctg ctctacttcc ttttttaata tttcttgccc
     2941 gaaggcactt acttatcagc cataacagcc ttgatctttt ctgtcagctg cggaacgatc
     3001 gtattcagat ctccgacaac gccataatct gccacatcaa aaatcggtgc atcttcatct
     3061 ttgttgatcg caacaataat atctgcttct tccattccgg ctacatgctg gatcgcaccc
     3121 gaaatgccga tcgcaaaata tacattcgga cgaacagtct tacctgtctg acctacctgc
     3181 agatccttcg gcttccatcc ggaatccacg accgcacggg aacaggatac cgttccgccg
     3241 atcgcctttg ccagatcttc caaaatttta aagttctccg gacttccgac tccacggccg
     3301 ccggatatca ggatctttgc gtccataata tcgaccgtat cggaaacaac tttgacaact
     3361 tccttaactg taacatattt tttatttggt acaaactcaa ctttgcactc ctccacaact
     3421 gcctttgcac ccttgatcgg atcgatcttc tcgccgccat aggccttggc aaccgccgcg
     3481 tcgatcacct tgatcatca
//
LOCUS       DAOSYE010000118         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19136_length_3491_cov_3.212456, whole genome shotgun sequence.
ACCESSION   DAOSYE010000118 DAOSYE010000000
VERSION     DAOSYE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19136_length_3491_cov_3.212456"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLU43_02930"
     CDS             complement(<1..1160)
                     /locus_tag="PLU43_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27393.1"
                     /translation="MKGRNIRCNNDRGSTIITVVVAVAFVAILVSVIMSVSMMNYKMK
                     AIDRRTKDDFYYDEKALNDVYTGIGQKCAEIMGESYEDILAQTSVQGASAYQNEEEAY
                     AAYKTEFLNQLRVSYLGTDMNVLQTILAAYIVESGGQKAEIRSIGSVQYESYDGETTY
                     SDPADGRRTRIKDLLITYQDENEYESSITLDIVIDVPSVDFFGSNNQIFDFSLVGDEG
                     IYITGGISSFSGKLYAGTHDAAETLDTQAGAADVYGGLNIYDMSLASLTSDCIVTRGD
                     INVRNAVFQMDNGETSDIWFESMRTVADGSNSAQIKGSGNFYALNDLELNALGSTVEL
                     DGSYYGYSDETFSTPLETKEHENTSLQSLKHTQSSAIIVNGKNSTLNLEGLDT"
     gene            complement(1162..2376)
                     /locus_tag="PLU43_02935"
     CDS             complement(1162..2376)
                     /locus_tag="PLU43_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO27394.1"
                     /translation="MTDLNKQIQSNRRNHDNRGFTLVELLVCIAILAIVFIPITKSFV
                     TAARTNGNAQSLQNATSLAQSVMEEIKMKSIADLKTSYAPTVALGVDVSSMDRYFQTC
                     YDTTDEDADVEYKLFEKDRTSSAGETFDVTATIVTTTYKQATSEAKADASDANSIELP
                     KLEEIDPAENASLSYEINAYDKAAVKNLQEQFQNGETDAPYDLVRAGGIKKLTITMTG
                     NALAGGTVYVTGKVSYSYGGKEIEYIVYNCSFAGDGINSISPDVYLFYSTMQNASSSS
                     YMENEEILIEDNTTGGVTHNAYFIMQDGETTLSPGNSKLTLTISRSSVSDSMDYTGAA
                     QNGLTVGTDPDDQNNMHLYSNLSYTDSGSTMEGHTYQSEAEDRIYEITVTLTKEGDSK
                     IYAQMTSTREVD"
     gene            complement(2389..>3491)
                     /locus_tag="PLU43_02940"
     CDS             complement(2389..>3491)
                     /locus_tag="PLU43_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27395.1"
                     /translation="KPFGDKTEQFVTKTADLKTQYQKIKDDYNKTDYYTQEIVKYQTA
                     TQELLSLFPADISQEHSILFISSTEEEVPMWLNQVRFEQNVSDIDSVDSDAGTQEENT
                     EAAEASTEQADTTVQSDETASALMQGLSGTRSDLTLEYEVSYDGFKKFLDYINHYDER
                     MVIRELTAEYDTDTGKVTGTVKLSEYALSGEGRTEEETEIPDINIGTSNVFQSGYSST
                     SISETGTETDDAAVNEAADFFLKVNAATDNTDGKVIGRSQDSDGETYISSNANKSEIV
                     TFTVEGSEGEYEASYEMAGEQYNNEEFTAEDQIVFKVISSVRTDSGDKVEVELKLVNK
                     ADIPLLVTIQNDDPNSPRVSVRDITGDVVLNQ"
BASE COUNT          844 a          906 c          681 g         1060 t
ORIGIN      
        1 gtatccagtc cttccagatt cagcgtgctg ttcttgccgt taacgatgat ggcactgctc
       61 tgtgtatgtt tcagtgactg cagtgaagta ttttcatgtt cctttgtttc aagcggagtg
      121 gaaaacgttt cgtcgctgta gccgtaatag ctgccgtcca gctccaccgt actgcccagt
      181 gcattcagtt ccagatcatt cagtgcataa aaattaccgc tccctttaat ctgtgcggaa
      241 ttagagccgt ctgcaaccgt acgcatcgat tcaaaccaga tatcggaagt ctctccgtta
      301 tccatctgga atacggcgtt ccttacgttg atatcgcctc ttgtaacaat acagtcactg
      361 gtaagagagg ccagcgacat gtcataaata ttcagtcctc cgtaaacatc ggcagcacct
      421 gcctgtgtat cgagtgtttc tgcagcatcg tgtgttccgg catagagctt cccgctaaag
      481 gaagaaatcc cgccggtaat atagatccct tcatctccta caagagaaaa atcaaagatc
      541 tgattgttgg aaccaaaaaa atcaacactt ggcacatcga tcacaatatc aagcgtgatc
      601 gaagattcat actcgttctc gtcctgataa gtgatcagga gatccttgat cctggtcctc
      661 ctgccgtccg ccggatcaga ataagtcgtc tccccgtcat aactttcata ctgcactgat
      721 ccgatactgc ggatctccgc cttctgtccc ccggactcaa cgatatacgc ggcaaggatc
      781 gtctgcagta cattcatgtc cgtgccaaga tagctgacac gcagctgatt tagaaattct
      841 gttttatatg ccgcataggc ttcttcttca ttctgatagg cagatgcacc ctgcacactt
      901 gtctgcgcaa gtatatcctc ataactttca cccatgatct cggcgcattt ctgtccgatg
      961 ccggtatata catcattcag cgctttttca tcataataaa agtcatcctt tgtacgcctg
     1021 tcaatagctt tcatcttata attcatcatt gacacgctca taataacgga aaccagaata
     1081 gcaacaaatg caacagcaac aactaccgtg ataattgtcg aacctctgtc gttattacac
     1141 cggatgtttc tgcctttcat atcagtccac ctccctggtc gaggtcattt gggcatatat
     1201 tttagaatcg ccttcttttg tcagtgtaac cgtaatctca tagatccggt cttctgcttc
     1261 cgattgatac gtatgtcctt ccattgtcga gccgctgtcc gtatagctta aattcgaata
     1321 aagatgcata ttattctgat catcgggatc tgtgccgaca gtcagcccat tctgcgcggc
     1381 acctgtataa tccatggaat ccgacacact gctccgcgaa atcgtcaggg taagcttact
     1441 gttcccggga ctcagtgtcg tttctccatc ctgcatgata aaataggcgt tatgcgtcac
     1501 accgcctgtt gtattgtctt cgatcaggat ctcttcattt tccatatagg aggaggaaga
     1561 agcattctgc atggtagaat agaacaaata cacatccgga ctgatggaat tgattccatc
     1621 cccggcaaaa ctgcagttat acacaatata ctctatttct tttcctccat aagaatagga
     1681 aactttcccc gtcacataca cggttccgcc tgcaagcgca tttccggtca ttgttatggt
     1741 cagctttttt attccgcccg ccctgactaa atcatacggt gcatcggtct caccattctg
     1801 aaactgttcc tgcagattct tgaccgcagc cttgtcgtat gcattgattt cgtatgaaag
     1861 cgatgcattc tctgccggat cgatctcttc cagctttggc agctcgatcg aattggcatc
     1921 ggatgcatcc gcctttgcct cacttgttgc ctgtttatac gttgtcgtta cgatcgttgc
     1981 cgtcacatca aaggtttctc cggccgaaga cgtccgatcc ttctcaaaca gtttgtattc
     2041 cacgtccgca tcttcatcag tcgtatcata gcatgtctga aaataacgat ccatggaact
     2101 tacatccaca cccaatgcaa cggtcggtgc gtacgatgtt ttcagatcgg cgatcgactt
     2161 cattttgatc tcttccataa cggactgcgc aagcgacgtc gcattctgta aagactgcgc
     2221 atttccattt gtacgtgcgg ctgtaacaaa acttttcgtg atcggtataa atacaatggc
     2281 aagtatcgca atacaaacga gaagctctac aagtgtaaag cctctgttgt catgatttct
     2341 tctgtttgat tgtatctgtt tgttcaaatc tgtcatgaaa cttccttctt actgatttag
     2401 gaccacatct ccggtaatat ccctgacaga tactctcgga gaatttggat catcattctg
     2461 tattgtcaca agcagcggaa tatcggcttt attgacaagc tttaactcta cctcgacttt
     2521 atcaccggaa tcggtgcgga cagaactgat tactttaaat acgatctggt cttctgcggt
     2581 gaattcctca ttattatatt gctctccggc catttcatag cttgcctcat attcgccttc
     2641 cgagccttca accgtaaacg tcacgatttc cgatttgttt gcatttgaag aaatgtacgt
     2701 ttccccgtca gaatcctgac ttcttccaat caccttcccg tccgtattat ctgtggcagc
     2761 attgaccttc aaaaagaagt cggccgcctc attgactgct gcatcatccg tctctgtacc
     2821 tgtttcggat atgcttgtgg atgaataacc ggactggaaa acatttgatg tgccaatatt
     2881 gatatccggt atttcagttt cttcttctgt ccttccttct ccgctgagtg catactcgga
     2941 cagcttaacc gttcccgtta cctttccggt atccgtgtca tactccgccg tcagttccct
     3001 gatgaccatc cgctcgtcat aatgattgat ataatcaaga aatttcttaa aaccgtcgta
     3061 cgatacttca tattccagtg taagatcaga gcgggtaccg gaaagaccct gcatgagagc
     3121 agaagccgtt tcatcagact gtacggtggt atctgcctgt tccgtgctcg cctccgcagc
     3181 ttccgtattc tcttcctgtg tccctgcatc ggaatccaca ctgtcgatat cagatacgtt
     3241 ctgttcgaac cggacctgat ttaaccacat cggaacctcc tcctctgtgg aagaaataaa
     3301 caagatcgaa tgctcctgtg aaatatccgc cggaaacaga gaaagcagtt cctgtgtcgc
     3361 cgtctgatac tttacgatct cctgcgtata ataatccgtt ttattataat catcttttat
     3421 tttttgatac tgggtcttca gatcagccgt ctttgtgaca aactgttccg ttttatctcc
     3481 aaaaggcttt a
//
LOCUS       DAOSYE010000119         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19148_length_3490_cov_2.983406, whole genome shotgun sequence.
ACCESSION   DAOSYE010000119 DAOSYE010000000
VERSION     DAOSYE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19148_length_3490_cov_2.983406"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLU43_02945"
     CDS             <1..460
                     /locus_tag="PLU43_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO27396.1"
                     /translation="LESTEDNRERYRAFLDVMEKNHLIFSQKQYYSGDYREKSGYTAA
                     KIMMLSGSMPEVVVCANDNMALGAIRAFKENGLKVPQDVAVTGFDDCDFAEVAGLTTV
                     NIPNYERGYMAAQCLFDFIRGNADNEILKISTTIKWRKSIGVVNKSEKNK"
     gene            682..2013
                     /locus_tag="PLU43_02950"
     CDS             682..2013
                     /locus_tag="PLU43_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27397.1"
                     /translation="MKKRKVISILLAAGMTISLLAGCGSGTADSSTTAADTDTGTEAV
                     TETADETTDEAEAADADDTVESADKIIVFQSKVEITDQMEALAEDYEEETGVEVEVWG
                     TTGDDYFQQLKTKLANNQGPTVFSLGPGAEADQMASYLTDLSDLSFADQIADGMAYTI
                     DDKVYGIPYTAEGFGLVYNKDLYDPSAVTDTDSLVSYLEEQKAAGISGLGLSSESYFM
                     IGHILNTPFALQDDPEGFLEELSKGEVKLADTEEFREFAELYAAIRENCTNPLEQTYD
                     KEIGDFATGKSATIHQGNWAASMFADYEIDFDMGLAALPLAGNDKLAVSVPTAWFVNS
                     QATPEEQQAGKDFLNWLYTSDTGIDYLMNQFGFIPVVDGMTNDNLDGLSQSVSEYIAD
                     GKTISWPMNDWPAGIVDVYLVPVAEEFFTTDMSAEDFLTKLDDAFAQAYSE"
     gene            2150..3046
                     /locus_tag="PLU43_02955"
     CDS             2150..3046
                     /locus_tag="PLU43_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27398.1"
                     /translation="MNNAKKKRAGKNAAWYILFTFPMVFAFTVIVIIPFIIGIGYSFF
                     SWDGLPLNPKIFVGGKNYVKLFSNTRFLSSAGHTLVFAIIAIVLINILGLAFALMCTS
                     KLKTRNIARTMFFMPYLIGGLILGYIWKFIFTDAFQMIGEATGLTGVFFNWLLDSNFA
                     LAAMLVVSTWQMAGYIMIIYITGITAIPDDVIEAAQVDGAGFWQTLFKIKFPLIMQSF
                     TICLFLTLSNCFKIFDVNLSLTGGGPNNATEMFAMNIYNEIFSLNNYGYGQAKAIVFF
                     LAVAAITMIQVSITKKKEVEMQ"
     gene            3043..>3490
                     /locus_tag="PLU43_02960"
     CDS             3043..>3490
                     /locus_tag="PLU43_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27399.1"
                     /translation="MTKNERKFSVKSVILNIVVIVLGLCYLFPIYIILVNSFKTRSEL
                     YDNVLALPKAFSFEFYEKAMDKMNFLRAFGNSLFVTLVSVLFIIVLSSMCAWMMVRSG
                     NTFSKFLFSVFVSTMLIPFQTLMMPLMQELGWIRDSLHIPVMDTLGG"
BASE COUNT         1060 a          595 c          856 g          979 t
ORIGIN      
        1 acttgaaagt acggaagata acagagaacg gtatcgggca tttttggatg ttatggagaa
       61 aaatcacttg atattcagcc agaaacagta ttattcaggc gattatcggg agaaaagcgg
      121 atatacggcg gcgaagatca tgatgctgtc aggcagtatg ccggaagtgg ttgtatgtgc
      181 aaacgacaat atggcacttg gcgcgattcg cgcatttaaa gaaaatggtc taaaggttcc
      241 gcaggatgtg gcggtgacag gatttgatga ctgtgatttt gcagaggtgg ccggactgac
      301 gactgttaat ataccgaact atgaaagggg ttatatggct gcgcagtgtt tgtttgattt
      361 tatcagggga aatgcagaca atgaaatttt gaaaatttcg actacgatca aatggagaaa
      421 atccataggg gttgtaaata aaagtgaaaa aaacaagtaa actagtaaaa tcaagtaaaa
      481 taattttaat aaagatagta aaaatactaa ataaactcaa caaaacgagt gctatttagt
      541 attttttttg gtaaaaaaca cagatattcg tcataataac ggaaaaacgg cagaatacat
      601 tgacatacag ggcgatatgt cttatcatat tagtataacg gtttaccaat taagttcatt
      661 tgatttaagg aggaaagtac tatgaagaag agaaaagtaa taagtattct actggctgca
      721 ggcatgacaa tatccttatt agcgggatgc ggctccggca cagcagatag cagcacgaca
      781 gcagccgata cggataccgg aacagaagca gtaactgaaa cggcagatga aacaacagat
      841 gaagcagagg cggcagatgc agacgataca gttgaaagtg ctgataagat cattgttttc
      901 cagtcaaagg tagagatcac agatcagatg gaagcattag ctgaagatta tgaggaagag
      961 acgggtgttg aagttgaagt atggggaaca acaggcgatg actacttcca gcagttaaaa
     1021 acaaagcttg caaacaatca gggaccaacc gtattctcac ttgggccggg tgctgaagca
     1081 gatcagatgg cgtcttatct gacagactta agtgatttgt cctttgcaga tcagattgca
     1141 gacggcatgg cctatacgat tgatgataaa gtatatggta ttccatacac agctgaaggg
     1201 tttggtcttg tatataataa agatctgtat gatccttccg cagtaaccga tacggattca
     1261 ctggtcagtt atctggagga gcagaaagca gcgggtatca gcggattagg attatcttcg
     1321 gaaagctact ttatgatcgg gcatatttta aatacaccat ttgcattgca ggacgatccg
     1381 gaaggatttc ttgaggagct ttccaaaggc gaagtaaagc ttgcagatac cgaagaattt
     1441 cgggaatttg ctgaattata tgctgcaatc agagagaatt gcacaaatcc gttagagcag
     1501 acctatgata aagaaattgg tgattttgca accggaaaat ctgctaccat tcatcagggt
     1561 aactgggcag caagtatgtt tgcggattat gagatagatt ttgatatggg actggcagca
     1621 cttccgcttg caggaaatga caaacttgca gtcagcgtac ctaccgcatg gttcgttaat
     1681 tcacaggcta ctccggaaga acagcaggcc ggcaaagact ttttgaactg gttatacaca
     1741 agcgatacgg gtattgacta tcttatgaat cagtttggat tcattccggt cgttgatgga
     1801 atgacaaatg ataatttaga tggattgtcc cagtctgtgt cggaatatat tgctgatgga
     1861 aagacgatct cctggccaat gaacgactgg cctgcaggta ttgtagatgt atatcttgtt
     1921 ccggtagcag aagaattctt tacaacagac atgagtgcgg aagatttcct tactaagctg
     1981 gatgatgcgt ttgcacaggc atatagtgag taattcggat accgatcgat aaataaataa
     2041 tgatctcata cagtggagtg cgaagcgtgg acagtgtccg ttcacactcc gctgttatca
     2101 tccggaggtt ctgcagggaa cagaagcgtc agaaaaggag aggtgcggga tgaacaacgc
     2161 aaaaaagaaa agagcaggaa aaaatgcagc atggtacata ttgtttacat ttccaatggt
     2221 gtttgcgttt actgtgatcg tgatcatacc atttataatc gggatagggt attctttttt
     2281 ttcctgggat ggactgccgc tgaacccgaa aatttttgtt ggaggaaaga attacgttaa
     2341 attattcagt aatacaagat ttttaagttc ggcagggcac acgcttgtat ttgcgatcat
     2401 agcgatcgtg ctgatcaata tacttggact ggcgtttgcg cttatgtgta caagtaaact
     2461 gaaaacaaga aatattgcaa gaacgatgtt ctttatgcca tatctgatcg gcgggctgat
     2521 actcggatat atctggaagt ttatttttac cgacgcgttt cagatgatcg gtgaggcaac
     2581 cggtctgacc ggagttttct ttaactggct gttagactca aattttgcac tggcagcaat
     2641 gctggttgtc agtacatggc agatggcggg ttatattatg attatttaca tcactggaat
     2701 cacggcgatc ccggatgatg tgatcgaggc cgcacaagtg gacggagcgg gtttctggca
     2761 gacacttttc aaaataaagt ttccgctgat catgcagtcc tttaccattt gtttattttt
     2821 gacattatcg aactgcttca agatctttga cgtaaacctt tcgctgacag gcggaggtcc
     2881 gaataatgca acggaaatgt ttgcaatgaa tatttataat gagatctttt ccctgaataa
     2941 ttatggatac ggacaggcaa aagcaatcgt attcttcctg gcagtagcag ctatcacaat
     3001 gattcaggtg tccattacga agaaaaagga ggtagagatg caatgacaaa aaacgaacgc
     3061 aaattttctg taaagtctgt catattgaat attgtagtaa ttgtgctcgg tctatgctat
     3121 ttatttccta tttatataat tctcgtgaat tcttttaaga cgagaagtga gttgtatgat
     3181 aatgtactgg ccctgccgaa agcattcagt tttgaatttt atgaaaaggc aatggataag
     3241 atgaatttcc tgcgtgcgtt tggcaactct ctgtttgtaa cactggtatc tgttctgttc
     3301 attatagtac tctcttcaat gtgtgcatgg atgatggttc gttccggcaa tacgttcagc
     3361 aagttccttt tcagtgtatt cgtttctacg atgctgattc ctttccagac actgatgatg
     3421 cctttgatgc aggaactggg ctggatccgg gattcgctgc acattccggt gatggatacg
     3481 ctgggtgggc
//
LOCUS       DAOSYE010000120         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19164_length_3488_cov_3.245267, whole genome shotgun sequence.
ACCESSION   DAOSYE010000120 DAOSYE010000000
VERSION     DAOSYE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_3488_cov_3.245267"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PLU43_02965"
     CDS             complement(<1..1346)
                     /locus_tag="PLU43_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HOO27400.1"
                     /translation="MRNNYKMLADNYFENISIVFKSLLVGLLAGGVTVLYRITLTYAE
                     ELSPKMYGFLKNNLTFLPLGILVLCLAAYFVGYLVSKNKMIAGSGIPQLEGILKGYFR
                     KRKSWLYTMFGKFVGGAIAIGAGLSLGREGPSIQFGACVAEGLGKKIGNGRLERKILM
                     ASGASAGLSAAFNAPMAGVVFALEEIFKYFSPVILLSMMSASVAADFVSKQVFGLTPI
                     FNFEITEEMPLDSYWLLILLGILLGVMGAFYNYLLLKTKKMFEKIKFLNTRTRVIVPF
                     LLAGVLGFVFPIVLGSGHKIIDELTLKNGVLFLVIIFIVKFLFSMVSYGSGVPGGIFF
                     PLIILGAVLGAAFAGVSINYFGFDKELYNNIIILAMAGYFTAIVRAPITGIVLIMEMT
                     GSFTHMLPLTVVAVTAYVVADLLKSPPVYEDLLDNMLQSENVKEENTNSITRVVLR"
     gene            complement(1477..2400)
                     /locus_tag="PLU43_02970"
     CDS             complement(1477..2400)
                     /locus_tag="PLU43_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOO27401.1"
                     /translation="MILSVSRRTDIPDYYAKWFLNRIREGYVYVRNPMNIHQVSKINI
                     TPDVVDCIVFWTKNPEPMLNKLEQLQEYSYYFQFTLTGYGNAMEPFLPSKRERILPVF
                     QQLSSMIGREKVIWRYDPIIITERYTEEYHLRAFREIASKLNGYCNKVIISFVDLYAK
                     TKKNMGEFPVTDRNQNELRRFSAQLAEIAKANGLKIETCAESIDLSSCGISSGSCIDK
                     KLIEKITGCQLKAGKDKNQRKECGCIESIDIGAYDTCQSGCKYCYANSSPETVRKNIA
                     LYDAFSPLLCSRITEKDKITERTVKSLKAGD"
     gene            complement(2487..2900)
                     /locus_tag="PLU43_02975"
     CDS             complement(2487..2900)
                     /locus_tag="PLU43_02975"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOO27402.1"
                     /translation="MQTERILMVLSGEVALLHEGEAEKVILEPGESYIFDGGSKTESF
                     GTCVDCNLMLKAGARGSMSYLSFNKKKPLTVLADADYIGFYLAEGTAEVELKNDFGKL
                     LRSDFAMIEKERDEEGFSIWVKGTGTVVAVKIFLE"
     gene            complement(2995..>3488)
                     /locus_tag="PLU43_02980"
     CDS             complement(2995..>3488)
                     /locus_tag="PLU43_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO27403.1"
                     /translation="AAELQAVSADHLLMISEQGILDLAQSSTIATLLPCTAFCLNQPY
                     APARKMIDAGCAVALASDYNPGSSFTNSIPLLFALSAIQMKLSLEEILCALTLNGAAA
                     IKKSKEIGSIEPGKRADLVVLSVPQYEFLVYHTCKNMVYEVIKDGKSVYKNTEPELMG
                     EKE"
BASE COUNT          977 a          884 c          586 g         1041 t
ORIGIN      
        1 aaacgcaaaa ccacccttgt aatactatta gtattttctt ctttgacatt ttcactttga
       61 agcatattat ccaaaagatc ctcataaaca ggagggctct ttaaaagatc cgctacgaca
      121 tatgctgtta cggcaacgac tgtcagcgga agcatgtgtg tgaacgagcc tgtcatctcc
      181 atgatcagaa cgatccctgt gatcggtgct ctgacgatcg ccgtaaaata gcccgccatc
      241 gcaagaataa taatattatt gtacagctcc ttatcaaatc cgaaataatt tatggaaact
      301 cccgcaaagg cagcgccaag caccgcaccc agaatgatca gcggaaaaaa gatgccgccc
      361 ggtactcccg aaccataact caccatggaa aaaagaaact tgacaataaa aattataacc
      421 aggaacaaca cgccgttttt caatgtcagt tcatctatta ttttatgtcc gctccccaat
      481 acgatcggaa atacgaatcc aagcactcct gccagtaaaa atggaacaat aactcttgtt
      541 ctcgtattca gaaactttat tttttcaaac attttttttg ttttcagcaa taaataatta
      601 taaaatgcac ccattacccc gagcaggatc ccaagcagaa tcagcagcca gtagctgtcc
      661 aaaggcattt cttccgtgat ctcaaaatta aaaatgggag tcagtccgaa tacctgcttt
      721 gatacaaaat cagccgctac cgatgcagac atcatcgaca gcaggattac cggagaaaaa
      781 tacttgaaaa tttcctccag cgcaaataca acaccagcca tcggcgcatt aaaagccgct
      841 gacagaccgg cgctggctcc gcttgccatc aatatttttc tttccagacg tccgttccct
      901 atcttttttc caagaccctc ggccacacag gcaccaaact gaatagaagg gccttctctt
      961 ccgagcgaaa gacctgctcc gatcgcgatc gctccgccta caaacttacc aaacatcgtg
     1021 tacagccagc ttttcctctt tctgaaatag cctttcagga ttccttccaa ctgcgggatt
     1081 ccactcccgg cgatcatctt atttttcgaa accagatatc cgacaaaata agcggccaga
     1141 cacagcacaa gtattccgag tggtaaaaac gtaagattat tctttaaaaa tccgtacatt
     1201 tttggactca attcttccgc ataggtcagc gtgatcctgt aaagaaccgt aactcccccg
     1261 gcaagcaatc caacaagcag acttttaaag acaatcgaaa tattctcaaa ataattgtca
     1321 gccagcattt tataattgtt tctcatttca tttcactcca aactcctcag ctcatatttt
     1381 ttcataattt tggaagccaa tgcttccaat tctttatttt ctgcctacaa aagtagatta
     1441 tatcaccatc cctgtcataa ttcaaaatat ttcttatcaa tcaccggctt tcagcgattt
     1501 tacggtcctc tccgttattt tatctttctc tgtgatccgg ctgcataaca gcggtgaaaa
     1561 agcatcatac aacgcaatgt ttttcctgac ggtttcagga ctgctattgg catagcagta
     1621 tttgcagccg ctttgacagg tatcgtatgc tccgatatca atgctctcta tacatccgca
     1681 ttctttcctc tgatttttat ccttccctgc cttcagctga cagcctgtga ttttttctat
     1741 cagcttttta tcaatacagc tgccggagct gatcccgcag gaggaaagat cgatcgactc
     1801 cgcacatgtc tcgattttta agccgtttgc ttttgcgatc tctgcaagct gcgccgaaaa
     1861 tcttctgagc tcattctggt ttctgtcggt cacggggaac tctcccatat tcttttttgt
     1921 tttggcatat agatccacaa aactgattat gactttatta caatatccgt tcaattttga
     1981 agcaatttca cgaaaggccc ttaaatgata ttcttccgtg tacctttccg taattatgat
     2041 cggatcatat ctccatatta ccttttccct gccgatcatg gaagataact gctgaaaaac
     2101 aggaaggatc ctctctcttt tagacggcag aaaaggttcc atcgcatttc cgtatccggt
     2161 aagtgtaaat tgaaaataat acgaatactc ctgcagctgt tctaacttat taagcatcgg
     2221 ctccggattt ttcgtccaga atacgataca gtctactacg tccggtgtga tatttatttt
     2281 gctgacctga tgaatattca tgggatttct tacatatacg tagccttctc tgatccgatt
     2341 cagaaaccac ttggcataat aatcaggaat atcagtccgc ctgctgacac ttaaaatcat
     2401 ttccgccacc tcggcaatct ctgcttttat gaaacgatcg ttatatgttt attatattgt
     2461 aaaaaagatt tgtacgtcaa ttatatttat tctaaaaaaa tttttactgc aacaactgtt
     2521 cccgttcctt ttacccatat ggaaaaaccc tcttcgtctc tttctttttc tatcattgca
     2581 aaatcagacc gaagcagctt tccaaaatca tttttaagct ccacctctgc cgttccctct
     2641 gcaagataaa atccaatata gtcagcatct gccaggaccg tcagtggttt ttttttatta
     2701 aatgaaagat agctcatact tcccctggca cctgctttta acatcaaatt gcagtcaaca
     2761 caggtgccga aactttctgt tttacttccg ccatcaaaaa tatatgattc gcccggctct
     2821 aaaatgactt tttccgcttc cccttcatgc agcagcgcca cttctccgga caataccata
     2881 agaatacgct ctgtctgcat taattcccat ccggtggata aagaaataat tccgtcgttg
     2941 tgcctccgga ccatttcgtt gttttctgtt tctcaaacgg gatgattttt agcttcattc
     3001 tttttctccc atcaattccg gttccgtatt tttatatacg ctttttccgt ccttgatcac
     3061 ttcataaacc atatttttgc atgtgtgata aactaaaaat tcatactgcg gaacagaaag
     3121 cacgaccaga tccgcacgtt ttccgggctc aatgctgccg atctctttac ttttcttaat
     3181 tgcagcagct ccgtttaaag tcagtgcaca caaaatttct tccagggaca gtttcatctg
     3241 aatagccgac agcgcaaaca gcagcggaat agaattcgta aaactgctcc ccggattata
     3301 atcacttgcc aatgccaccg cacaccccgc atcgatcatt tttctcgcag gagcatatgg
     3361 ctggttcaga cagaaagccg tacagggcag cagcgtcgcg atcgtgctgc tctgtgccag
     3421 atctaaaatt ccctgttcgg agatcataag aagatgatcg gcggaaacag cctgaagctc
     3481 tgccgcaa
//
LOCUS       DAOSYE010000121         3478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19246_length_3478_cov_2.436459, whole genome shotgun sequence.
ACCESSION   DAOSYE010000121 DAOSYE010000000
VERSION     DAOSYE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19246_length_3478_cov_2.436459"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1268)
                     /locus_tag="PLU43_02985"
     CDS             complement(90..1268)
                     /locus_tag="PLU43_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO27404.1"
                     /translation="MADTRAIDLKYLCTAIGNMSGIPVRIYQNNRLSFFHSLVTLPKD
                     PILLHEHDILAVTDAIGYYITPAFDYYGIAHIQKNTVVIGPSGMSVKTEQELRKEAFE
                     LAVDPEKTEEFLSSMKLIVHMPLESLLQILCTVYYVMTGQKTSLTDLAILASAQKQLS
                     RELTFETSSGMDNPHNTFRTEQQLLDIVRRGDTIALDSWSSQAPAVRPGKVAPDMLRQ
                     IKNTFIITATLVSRAAIRGGMDVDDAFRLSDSYIQKCEPLYRFDQITNLQFHMVRHYT
                     STVEELRYSRNQSELTASVASYIRHHLSDAIKTKDIADTLFISRSRLSTRFKAECGMN
                     LSEYIHYQKMSEAKHLLAHSDKSLLLISTYLGYSSQSHFCRIFKKYAGMPPAEFRSRH
                     "
     gene            1405..2868
                     /locus_tag="PLU43_02990"
     CDS             1405..2868
                     /locus_tag="PLU43_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO27405.1"
                     /translation="MRLLEKKFFCSRVKTAEVLKREKWLGYLLGPSGALLLNAVLATY
                     LNVYYTDVLNLTGVLGGAFLMIFPITSKIIDAVTNVLMGYIIDRTKTKQGKARPWLLF
                     SAPIVCITGILLFTVPTGSTAIKVVWVMLSYNLYYSVGYTMFNMSHGLMVPLSTRNST
                     ERGSLSVFNQIATIMMSGILVALVFPMAVMPYLGVDQSKWICVMSVLSILAMPLIMLE
                     YFFTKERVTEEEMELEEKSVPLKRQLKIILSDRYMLLIYAYFLIYTVGISFKNLSLVY
                     YCNYVLGSYNDGITQTMVSVIGGIPMGIGVFAVWPLAKRFGKRNVTMADFVLYVIGGI
                     ISWSMPSDMGTVLVGQFIKNIGGLPSAYVFMALFADVLDHVEWKGNIRCDGIAMSVYN
                     IIAVAGVGVCTGIFNMVLSKTGYIAPGLDAARETVAAVQNAAVKGAITFSYTGLEVFT
                     GLVLIALLFFLDVEKSIDQKQEEIRKRKGIDVKEEEK"
     gene            2865..>3478
                     /locus_tag="PLU43_02995"
     CDS             2865..>3478
                     /locus_tag="PLU43_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOO27406.1"
                     /translation="MKSVRLRTEYLSNPQGIDIAVPRLSWNCTDGKIQSAYRIKAWDE
                     EGSSVWDTGKVLSGQMVHILWNGRKLKSRDHISWKVRLWEEAEEPGEWSQEAFFEMGF
                     LADHDWKASWITGNYMVNRYRRYPVDCFRKKFHIYKEVKKARLYATACGLYELCLNNT
                     RVGDFVLAPGITDYNKRVQYQTYDVTALLLSQENEMTLMLADGW"
BASE COUNT          944 a          658 c          933 g          943 t
ORIGIN      
        1 gtgtcacagc catgttacgt tttgattata ttttgcagga ttattgcccg ggctttccgg
       61 cagcctgata gcacaggtaa agcggacatt cagtgacggc tgcggaattc cgcaggcggc
      121 ataccggcat attttttgaa gatacggcag aaatggctct gtgaggaata gccaagatac
      181 gtactgatca ataataaact tttatcggaa tgagcgagca gatgttttgc ctcgctcatt
      241 ttttgatagt gaatatattc cgataagttc atgccgcatt ctgctttaaa acgcgtggat
      301 aaacggctcc ggctgataaa cagcgtatct gcaatgtctt ttgttttgat cgcatcagat
      361 aaatgatgac ggatatagga tgcgacactg gcagtcagtt ccgactgatt ccggctgtat
      421 cttaattcct ccacagtact tgtgtaatgc cgtaccatat ggaactggag atttgtgatc
      481 tggtcgaaac gataaagcgg ttcacatttt tgaatatagg aatcacttag acggaaggcg
      541 tcgtctacat ccattccgcc gcggatcgca gctctggaaa caagggtcgc ggtaatgata
      601 aaagtatttt tgatctgacg gagcatatcc ggtgctactt tgcccggacg aaccgcaggt
      661 gcctggctgc tccagctgtc cagcgctatt gtatcgccgc ggcggacgat atccagaagc
      721 tgctgttcag tgcggaatgt attgtgagga ttgtccatgc cggaggatgt ttcaaaagtc
      781 agttcccggc tcagctgctt ttgtgcggat gcaaggatcg ccagatcggt cagacttgtt
      841 ttctgaccgg tcatgacata atagacagta cataatattt gcagaaggct ttctaacggc
      901 atgtgtacga tgagcttcat ggaagacaga aactcctctg ttttttcagg atcaacagca
      961 agctcgaagg cttctttccg aagctcctgt tctgttttta cagacattcc ggaaggaccg
     1021 atcacgaccg tgtttttttg tatatgtgca ataccgtaat aatcgaatgc aggggtgata
     1081 taatagccga tcgcatccgt cacggcaagg atgtcatgct cgtgaagaag gatcgggtct
     1141 tttggaagcg taacaagcga atgaaaaaaa gaaagccggt tattttgata aattcggaca
     1201 ggaattccag acatatttcc aatagctgtg cagaggtatt taagatcgat cgcacgagtg
     1261 tctgccatta tagatgcatc ctttcaaatt gaatgagcag tataaaacaa atatactata
     1321 tataaaacaa atatgcagga cgtgaaaagg aaaacagatt aaaataatgg tgtaagtgat
     1381 gataaatatg caggagaaaa aagaatgaga ctgttggaaa aaaagttttt ttgcagccgg
     1441 gtcaaaacgg cagaagtatt aaaaagagaa aaatggctcg gctatctgct cggaccgagc
     1501 ggggcgctgc tgctcaatgc ggttctggcg acttacctga atgtatatta tacggacgta
     1561 ctgaatctga ccggcgtatt agggggagct tttctgatga tctttccgat cacgtccaag
     1621 ataatcgatg cggttacgaa cgtactgatg ggatatatca ttgaccggac aaagacaaaa
     1681 cagggaaaag cgcgtccgtg gctgctcttt tcggctccga tcgtatgtat taccgggatc
     1741 ctgctgttta cggttccgac cggcagcact gcaataaagg ttgtctgggt aatgctttcc
     1801 tataatttat attattccgt tggatatacg atgtttaata tgagccatgg attgatggtg
     1861 ccgctgtcaa cgcgaaattc gacggaacgg ggcagtcttt cggtgttcaa tcagatcgcg
     1921 acgatcatga tgagcggtat tctggttgcg cttgtttttc cgatggctgt tatgccgtat
     1981 cttggcgtgg atcagtcaaa atggatctgt gtcatgtccg tattgtcgat tctggcaatg
     2041 cctctgatca tgctggaata ttttttcaca aaggaacgtg tgacagagga agagatggag
     2101 ctggaagaaa aatccgttcc gctgaaaaga cagctgaaaa ttattttaag cgaccggtat
     2161 atgcttttga tctatgcata ttttcttatt tatacggtcg gaatcagctt taaaaatctt
     2221 tccttagtat attattgtaa ttatgttctg ggatcttaca atgacgggat cacacagaca
     2281 atggtctccg tgatcggcgg gatcccgatg ggtatcggtg tgtttgcggt atggccgctg
     2341 gcaaagcggt ttgggaaacg aaacgtgacg atggcagact ttgtgctcta tgtgatcggc
     2401 ggaatcattt cctggagcat gccgtcggat atgggcacag tgctggtcgg acagtttatc
     2461 aagaatatcg ggggactgcc gagtgcctat gtgttcatgg cgctgtttgc ggatgtgctg
     2521 gatcatgttg aatggaaagg aaatataaga tgtgacggga tcgcgatgtc tgtttacaac
     2581 atcattgcgg tcgctggtgt aggtgtctgc accggtattt ttaatatggt gctttctaag
     2641 acgggatata tcgcgccggg tctggatgcc gcaagggaga cagtagcggc agtacaaaat
     2701 gctgccgtaa agggggcgat cacgttttcc tatacgggtc tggaagtgtt taccggtctt
     2761 gtactgatcg ctttgctgtt ctttttggac gtggaaaagt cgatcgatca aaaacaggaa
     2821 gaaatcagga aaagaaaagg aatagacgtg aaggaggaag aaaaatgaaa tcagttcgtc
     2881 tgagaacaga atacttatcc aacccgcagg gaatcgatat tgcagttccg cggctgagct
     2941 ggaattgtac ggatggaaaa atacagagcg cttataggat caaggcgtgg gatgaggaag
     3001 gatcttcggt atgggatacg ggaaaggtct tgtccggaca gatggtgcat attttatgga
     3061 acggcagaaa attaaaaagc agggatcata tcagctggaa agtccgttta tgggaggaag
     3121 cagaggaacc gggcgagtgg tctcaggaag ctttttttga aatgggtttt cttgccgatc
     3181 atgactggaa agcaagctgg atcaccggaa attatatggt aaacagatac aggcggtatc
     3241 ccgtggattg ttttcggaag aaattccata tatataagga agtaaaaaaa gcgcggctat
     3301 acgcaaccgc ctgcggtctg tatgaacttt gcctgaataa tacgcgtgtt ggggactttg
     3361 tactggcacc agggatcacg gattataaca agcgtgtgca gtaccagacc tatgatgtga
     3421 ccgcactgct cctttcgcag gagaatgaga tgacactcat gctggcggac ggctggta
//
LOCUS       DAOSYE010000122         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19255_length_3477_cov_2.876973, whole genome shotgun sequence.
ACCESSION   DAOSYE010000122 DAOSYE010000000
VERSION     DAOSYE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19255_length_3477_cov_2.876973"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="gmd"
                     /locus_tag="PLU43_03000"
     CDS             complement(<1..719)
                     /gene="gmd"
                     /locus_tag="PLU43_03000"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO27407.1"
                     /translation="MRNVALITGITGQDGSYLAELLLEKGYEVHGIIRRHSSISTERI
                     DHLYYDREIRDKTFFLHHGDLTDSSNLNRLIEQIQPTEIYNLAAQSHVAVSFEVPEYT
                     AETTGIGTLRLLDAIKKSGVHCKFYQASTSELFGGLPETAPQSEKTPFYPKSPYGAAK
                     LYAYWITVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVASIMADEREKLSLG
                     NLDAKRDWGFAGDYVEGMWRIL"
     gene            887..1789
                     /locus_tag="PLU43_03005"
     CDS             887..1789
                     /locus_tag="PLU43_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO27408.1"
                     /translation="MTSSNSYMLENSHASATSRSDRVIATPTKFAQNNYFYVQEVGRL
                     KSTIPHLSKRQNLNSFLFFIVLSGEGIVTYNGQAFHLEKGGCVFIDCGQNYSHISSEE
                     APWELMWVHFNGTKAHEFYQQYIRVSGQNIFRAVSVVPYEQTLSDLLRINKDRSTMTE
                     LQSNKLITDIITRCFLETRRDAADTADNSMRMKLDQVRSYIDANFTEKILLEDLAERF
                     YISKYHLSREFKKTYGITIGTYLLSCRIGLAKQLLRFSSKPVEEIAASCGIFDTSYFT
                     KVFRKSEQMTALEYRKKWGAAGTR"
     gene            1942..2412
                     /locus_tag="PLU43_03010"
     CDS             1942..2412
                     /locus_tag="PLU43_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOO27409.1"
                     /translation="MSFLRELVNNKVFLIAALGWAVAQLLKTIINLCFTKKFDPERLV
                     GSGGMPSSHSSTVCALATASAMQYGAGSFEFAISAVLAIIVMYDARGVRRETGTQAVV
                     LNQLIDYFSNIENSQHKLEFTQKKLQELVGHTPLQVAAGAILGILIALSLYPNL"
     gene            complement(2407..3282)
                     /gene="rfbD"
                     /locus_tag="PLU43_03015"
     CDS             complement(2407..3282)
                     /gene="rfbD"
                     /locus_tag="PLU43_03015"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOO27410.1"
                     /translation="MKKIIVTGCNGQLGRAINKLYAKNTEYELVNTDFKVPGTIDLDI
                     TDLDAVLKLFLEVKPYAVINCAAHTAVDACEVQADRAYKINAIGPRNISIAAAKTGAK
                     LMHVSTDYVFAGDGNRPYTEFDPAGPKAMYGITKLAGENFVKEFAKDYFIIRTAWLYG
                     DGKNFVKTMLRLSETHQTLTVVNDQIGSPTSAAELARAIACLLPTENYGLFHGTCEGQ
                     CSWADFAKEIFRLAGKPTEVVPVTTKEYLEMNPDSARRPAYSVLDNYMFRLTTDFSFA
                     PWQEAIAEYMKEFKL"
BASE COUNT          937 a          863 c          708 g          969 t
ORIGIN      
        1 aggatcctcc acataccttc tacataatca ccggcaaatc cccagtcacg tttggcatcc
       61 agattgccga gagagagctt ttctctttcg tccgccatga tcgaagcaac tgcacgcgta
      121 atttttcttg ttacgaatgt ttcgccgcgt cttggcgatt catgattgaa taaaataccg
      181 ttgcaggcaa acagattata ggcctccctg taattgaccg tgatccagta agcatacagc
      241 tttgcggctc catacggact tttcggataa aacggcgtct tttcgctctg aggggctgtt
      301 tccggaagtc ctccgaacaa ttccgatgtg gatgcctgat agaacttaca gtggacaccg
      361 cttttcttaa tggcatccag cagccttaat gtaccgattc ccgttgtctc tgccgtatat
      421 tccggaacct caaaggaaac ggcaacatgg gactgcgctg caagattata aatttcagtc
      481 ggctggatct gctcgatcag acgattcaga ttactcgaat ctgtcagatc cccatgatgc
      541 agaaaaaatg ttttatccct gatctccctg tcataataca aatgatcgat cctttccgta
      601 ctgatactgc tgtgcctgcg gataatgcca tgtacctcat atcccttttc caaaagcagt
      661 tcggcaagat aagaaccgtc ctgacctgta attcctgtaa tcaatgcgac atttctcata
      721 tgaaatctcc ttatatgtat tctattttgt aatttttgtt cttttttgca ctggcaaaag
      781 tcagtatact cctttataat acaagtatct acgttaaaaa ttgctatata atagtaaaat
      841 attgcgccaa atgatccgtc tatattttct aatgaaagga acgcctatga catcatcaaa
      901 ctcctatatg ctggaaaaca gccatgcttc ggcaacttcc cgttcggacc gcgtgatcgc
      961 aacaccgaca aaattcgcac aaaataatta tttttatgtc caggaagtcg gacggctgaa
     1021 aagtaccatc ccccatcttt ctaaaagaca gaatctgaat tcctttttgt ttttcatcgt
     1081 tctgtccggc gaaggcatcg tcacctataa cggacaggcc tttcatctgg aaaagggcgg
     1141 ctgtgtcttt atcgactgcg gtcagaatta ttctcatatc agcagtgaag aggccccctg
     1201 ggaactgatg tgggtacatt ttaacggaac aaaggcacac gagttttatc agcagtacat
     1261 ccgcgtaagc ggacagaata ttttccgcgc agtttctgtc gtcccgtatg aacagacgct
     1321 ctcggacctg ctgcgcatca acaaagaccg gtctaccatg actgagctgc agtccaataa
     1381 gctgatcacg gatatcatta cccggtgttt cctggaaacg cggcgtgacg ctgcagatac
     1441 tgcagataat tccatgcgca tgaagctcga tcaggtacgc tcctatatcg atgccaattt
     1501 cacggaaaaa attttactgg aagatcttgc ggaacgattt tatataagca aatatcattt
     1561 gtcaagagaa ttcaaaaaga catacggtat cacaattggt acttatctgc tgagctgccg
     1621 gatcggactt gcaaaacagc tgcttcgttt cagcagcaaa ccggttgaag aaattgcagc
     1681 ttcctgcggc atctttgata caagctattt tacaaaagta ttccgcaaat ccgaacagat
     1741 gactgccctg gaatatcgga aaaaatgggg tgcggcaggt acacggtaat cgtattgaaa
     1801 aagcccggat attttacata gaataaattg tcatcctgca gggtatatgc tatacttaac
     1861 aggcacgagt atatgcggca gccaaaacgg tccgcacaga aatcaaatac ttgcaaggaa
     1921 tgatatgaaa agaggcattg tatgtcattt ttaagagaat tggtcaataa taaggttttt
     1981 ctgatcgctg cactcggatg ggctgttgcg cagcttctga aaacaattat caacctttgc
     2041 ttcacaaaaa aattcgatcc ggaaagactg gtcggttccg gcggaatgcc aagctcccat
     2101 tcctctaccg tatgtgcgct tgccacggct tcagccatgc agtacggcgc aggttccttt
     2161 gaatttgcaa tatcggctgt gcttgccatc atcgtcatgt atgatgcaag aggtgtccgc
     2221 agggaaaccg gcacacaggc cgttgtactg aatcagctca tcgattattt ttccaacatt
     2281 gaaaactccc agcacaagct ggaatttaca cagaaaaaac tgcaggagct tgtaggacac
     2341 actcccctgc aggtagcagc cggtgcgatt cttggaatcc tgatcgcact ttccctttat
     2401 ccgaatctat aatttaaatt ctttcatata ttccgcaatc gcttcctgcc atggcgcaaa
     2461 cgaaaagtcc gttgtcagcc tgaacatata attatccagc accgaatagg caggtctcct
     2521 tgcggaatcc ggattcatct ccagatactc ttttgttgtg accggtacga cttctgtagg
     2581 ctttccagcc agccgaaaaa tttcctttgc aaaatcagcc cagctgcact gcccctcaca
     2641 ggtgccgtga aataacccgt aattttccgt cggaagcaga caggcgatcg cacgggcaag
     2701 ctctgctgca ctcgtcgggg aaccgatctg atcatttacg accgtaagcg tctgatgcgt
     2761 ctcggacagc cgcagcattg tcttgacaaa attctttccg tcaccataca gccatgcggt
     2821 acgaataata aaataatcct ttgcaaactc cttaacaaaa ttctcaccag caagctttgt
     2881 aataccatac atcgcttttg gcccggccgg atcaaactcc gtatacggcc tgtttccgtc
     2941 gccggcaaat acataatcgg tagaaacatg catcagcttt gctcccgtct ttgctgccgc
     3001 aatactgata tttctcggac cgatcgcatt gatcttgtag gctctgtccg cctgcacctc
     3061 acaggcatct actgccgtat gcgccgcaca gtttatgacc gcataaggtt tcacttccaa
     3121 aaacagcttc agcaccgcat ccagatccgt gatatccaga tcgatcgtac caggcacctt
     3181 aaaatccgta ttgaccagtt catactccgt attttttgca tacagcttat taatcgcacg
     3241 tcccagctgg ccattgcagc ccgttacaat aatttttttc attgccctgt ccttctttcc
     3301 ggcacagata aattgccctt ttctcggttt ctaaaatata gtatactgaa agcggtgttt
     3361 atttgcaaat gatttcttat aaagactatt catgacggag gcagctatat gggcatttat
     3421 ttaaaaaaac atcggtatct tttttatatt gcaggggtct cgctcctgca cctttgc
//
LOCUS       DAOSYE010000123         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19256_length_3477_cov_2.318527, whole genome shotgun sequence.
ACCESSION   DAOSYE010000123 DAOSYE010000000
VERSION     DAOSYE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19256_length_3477_cov_2.318527"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..665
                     /locus_tag="PLU43_03020"
     CDS             63..665
                     /locus_tag="PLU43_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HOO27411.1"
                     /translation="MNKDLEKIIKETAGEVGKIEYINSSKIPGIDLYMDQVTTFMEEH
                     LSPSKRYDDDKILTKTMINNYAKNELIPPPVKKKYNKEHILLLTFIYYFKNLLSINDI
                     QKLLSPLVDKFYQNKESDISLTDLYDEIFKMCDEKREILQESVARNLQTAESTFENAA
                     EEDRDYLQLFAFIGILSQDAYAKLLIIEKLLDTLPEPTEP"
     gene            complement(682..984)
                     /locus_tag="PLU43_03025"
     CDS             complement(682..984)
                     /locus_tag="PLU43_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOO27412.1"
                     /translation="MNPKIRTEAVDYLFDAVMQLKSKEECYIFFEDICTINELLSLSQ
                     RFEVASMLREKKTYLDIAKKTGASTATISRVNRSLNYGNDGYDMVLERMKAKTQEP"
     gene            complement(1158..1778)
                     /locus_tag="PLU43_03030"
     CDS             complement(1158..1778)
                     /locus_tag="PLU43_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOO27413.1"
                     /translation="MGAYENLREYHLDVMREVGNIGAGNACTALSVLLGTQIDMSVPS
                     VHLLGFDSTSKYLGGGAHKVLGIRIDVSDDLEGMMLHIIKKRFAERIINTFYEKKLDD
                     FDCMDEMDASVLSEMANITSGAYANSIATLTDMQINIGAPIKTKSMTVGDILKIPFES
                     FSALSDKILIIDEQFTIGDEQITSNMILILENSSLTKLFKKLEIKE"
     gene            complement(1867..2709)
                     /locus_tag="PLU43_03035"
     CDS             complement(1867..2709)
                     /locus_tag="PLU43_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOO27414.1"
                     /translation="MKMRTEAGIYSGFAAGSMAAAGLFLLLLLLIKPNRKRDVSGFKG
                     KMYAHRGLHDDKIPENSMAAFLAAKEHGYGVELDVQMTKDDQLVVFHDGSLWRMCGVR
                     GKLKDYTYKELQRFSLKDTEEKIPLFSDVLKLLDKTDIICELKNNNGNFNYYFCERVC
                     EEIKNYKGNICIESFSPFLLRWFYKNRPEIIRGQLSNHMAHGDGMHFPANLFMTHLLV
                     NRVSRPDFISYRFSDIRTFGYRLCRKLYAPFCVVWTVKGQKQIRDAKKNFDTIIFEKS
                     SANR"
     gene            2886..>3477
                     /locus_tag="PLU43_03040"
     CDS             2886..>3477
                     /locus_tag="PLU43_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27415.1"
                     /translation="MKKKLVSMMLVAAMAAVTLAGCGSSASTADSAADTTETTEAADA
                     ADTTEAAETTEADDTAAAGAAETFLIGGIGPLTGGAASYGISVKQGAEVAIAEINSAG
                     GVTAGDTTYQLALAFEDDEASEEKSPQAYNTLMDQGINALMGTVTSGACLAITDLTYE
                     DNILQITPSGSAQDCTNNPNAFRICFTDPLQGETMAD"
BASE COUNT         1023 a          795 c          647 g         1012 t
ORIGIN      
        1 agtttttaaa actatataat aatatgtgat gatgaaatca aacaagtaat gagggttcgg
       61 acatgaataa ggatttggaa aaaatcataa aagaaactgc cggagaagtg ggtaaaatcg
      121 aatatatcaa ttcgtccaaa attccgggaa tcgatttata tatggatcag gtcactactt
      181 ttatggaaga gcatttaagt ccctccaagc ggtatgacga cgacaagatc ctgacaaaga
      241 cgatgatcaa caattatgcg aaaaacgaac tgatcccgcc gcctgtcaaa aagaaatata
      301 acaaggagca tattcttctg ctcacgttta tttattattt taagaatctg ctttccatca
      361 atgatatcca aaagcttctt tccccgttag tcgacaagtt ctatcaaaat aaagaatccg
      421 atatatcatt aacggatctt tacgatgaga tctttaaaat gtgcgatgaa aaaagggaga
      481 tcctgcagga aagtgttgca cgaaatctgc agaccgccga aagcactttt gaaaatgcag
      541 cggaagaaga ccgcgattac ctgcagctgt ttgcatttat cggtatctta agccaggatg
      601 cctatgcaaa actgctgatt atagaaaagc ttttagatac gctcccagag cctaccgaac
      661 cttgatcgtc caatttgggg atcagggttc ctgtgttttt gctttcatgc gttctagtac
      721 catatcatat ccgtcgtttc cataattcag ggaacggttt accctgctga tcgtagcggt
      781 cgaagcgcct gtctttttag caatatccag atatgtcttt ttttcccgca gcatactggc
      841 cacttcaaaa cgctgtgaca aagacagcag ttcattgatc gtacaaatat cttcgaaaaa
      901 aatataacac tcctctttgc tttttaactg cataacagca tcaaataaat agtcaacggc
      961 ttctgttctg atttttggat tcataattat gcctgccttt cggataaact ataatagata
     1021 acactttaac acataaacat tttatcactc tgaagtggtc tagtaaagcg gtttttcata
     1081 ccccttctgt aataaaaaca aatcaggacc gccggctcta tgcccgcggt cctttccaat
     1141 acttgtcttt ttgatttcta ttcctttatc tccaattttt taaacagctt cgtcagggaa
     1201 ctattttcca atattaaaat catattgctt gtgatctgtt cgtctcctat cgtaaactgc
     1261 tcgtcaatga tcagtatctt atcactcaat gcggaaaaac tctcaaacgg tattttcaga
     1321 atatctccaa ctgtcatgct ttttgttttg atcggtgctc cgatattgat ctgcatatcc
     1381 gtcaatgtcg caatggaatt ggcataagca ccggaagtaa tattagccat ctcactcaat
     1441 acggaagcat ccatttcatc catacagtca aaatcatcca gctttttttc ataaaatgta
     1501 ttgatgattc tctcggcaaa gcgtttcttt ataatatgta acatcatgcc ttccagatcg
     1561 tcactgacat cgatccgtat gccgagcact ttatgcgctc ctcctccgag gtatttggaa
     1621 gtagaatcaa atcccagcaa atgaacactt gggaccgaca tgtctatctg cgttccaagc
     1681 aggaccgaca atgccgtaca tgcattaccg gctccaatat tgccgacttc ccgcattaca
     1741 tccagatgat actctctcag attttcataa gcgcccatat ggcactcctt tctcatcaac
     1801 aaattatggt taatttactt ttctttaaca gtttatcata attttattgt aagggcaatg
     1861 tcagaactac ctgttggcgc tacttttttc aaaaataata gtatcaaaat tcttttttgc
     1921 atccctgatc tgcttttgac ctttcacggt ccatacaacg cagaacggtg catacaattt
     1981 tctgcataaa cgatagccaa acgttctgat atcagagaac cggtaagaaa taaaatccgg
     2041 tctggatacc cggttgacaa gcagatgtgt cataaacaga tttgcaggaa aatgcatacc
     2101 gtctccatgg gccatatgat tggacagctg ccccctgata atttccggtc tgtttttata
     2161 aaaccagcgc agcagaaacg gactaaacga ttcgatgcag atatttcctt tataattttt
     2221 aatttcttca catacgcgtt cacaaaaata ataattgaaa ttgccgttat tattcttaag
     2281 ctcgcagata atatccgttt tatcaagcag cttcagcaca tccgaaaata acgggatctt
     2341 ctcctctgta tccttcagag aaaaccgctg cagctcctta tacgtatagt ccttcagctt
     2401 ccctctcaca ccgcacatac gccacagact gccgtcatga aaaacaacaa gctgatcgtc
     2461 ttttgtcatc tgcacatcca gctccacacc atagccgtgc tctttagcag ccaaaaaagc
     2521 agccatggag ttttccggga ttttgtcgtc gtgaagtccc ctgtgtgcat acatctttcc
     2581 tttgaatccg gaaacgtctc ttttccggtt tggtttgata agcaataata atagaagaaa
     2641 caaaccggct gccgccatgg agccggctgc aaaaccgctg tagatccctg cttctgttct
     2701 cattttcatt atattcaccg ctttcggctt ttaattgtat cactatcccg ttttattttc
     2761 aataaaatct catctcctgc agatattctg tacataaacc aataaataat tgacaatatc
     2821 ttcataaacg gttattatgt gaaggataat atctattcta tcacatttaa caaggaggaa
     2881 acattatgaa aaagaaatta gtaagtatga tgctcgttgc agccatggct gcagtaacac
     2941 ttgcaggatg cggctcctct gcttccacag cagacagtgc ggcagacaca acagaaacga
     3001 cagaggctgc ggacgctgca gatacgacgg aagccgcaga aaccacagag gcagacgaca
     3061 cggcagctgc cggtgcagca gaaacatttt tgatcggcgg tatcggacct ttaacaggcg
     3121 gtgcggcttc ttatggtatt tccgtaaagc agggcgctga ggttgcgatt gctgagatca
     3181 actcagcagg cggcgttacg gcaggcgata caacttatca gcttgcgctt gcatttgaag
     3241 atgacgaagc ttccgaagag aaatccccgc aggcttacaa cacattaatg gatcagggta
     3301 tcaatgctct tatgggaacc gttacatccg gcgcatgtct tgcgatcaca gatctgactt
     3361 atgaagataa catccttcag atcactccgt caggttctgc tcaggactgt acaaataatc
     3421 cgaacgcatt cagaatctgc ttcaccgatc ccctgcaggg tgaaacaatg gccgatt
//
LOCUS       DAOSYE010000124         3462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19365_length_3462_cov_3.118873, whole genome shotgun sequence.
ACCESSION   DAOSYE010000124 DAOSYE010000000
VERSION     DAOSYE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19365_length_3462_cov_3.118873"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..478
                     /locus_tag="PLU43_03045"
     CDS             209..478
                     /locus_tag="PLU43_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27416.1"
                     /translation="MMYLHFCTDCRQTYILSGHQQECVKCGGTLVELKLSYQDYVDYS
                     PAQRRQLILELEDPEELARQKRHYRFSKQTKRYKEWMKRKEDISR"
     gene            complement(500..877)
                     /locus_tag="PLU43_03050"
     CDS             complement(500..877)
                     /locus_tag="PLU43_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HOO27417.1"
                     /translation="MKEEVKLELYVSEVLRELGVPAKLLGYRYLREAIIMLIHDMEIA
                     SSITKILYYDIAKAHNSSTDRVERAIRTAVGISWEQGNPEVFERIFGYSIMSSRKKPC
                     NSEYMVQIADKIHLDRKYNMLVG"
     gene            complement(985..2304)
                     /locus_tag="PLU43_03055"
     CDS             complement(985..2304)
                     /locus_tag="PLU43_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOO27418.1"
                     /translation="MEAGFLGEFPWVLCAYFYTLMESLAIRDSFDIALYRKKIKQSYY
                     ILAVMLLFLWMIIADEAASKSYEKSILILLFAFGMVFILYEGKVWQKILTVIAVSLVC
                     LLFDLVNLQIMSHLLDEDIGTFAGNDVYLYYMALTSKLTQMLLIKMLKCFVGRNKIKA
                     MYKIDRVLCILVPIAIMLNAYTSFMKNLIRKDITEWDIYGILLLVFLGSMLYFIMYRM
                     GEYYDEMSRSKVLDNQMRSEIEYMESLQQTHKKIRQIAHGFENQLSIVNTLLKNEEYD
                     KAKEYLSEISNAVEKNVLPVHTNNIVIDAVLNQMFIVAVSKKITMNFNIQDLGTLRMN
                     VSDMAAILNNALNNAIEACDKLPGGTPRIIEIQISNEEQELIISIQNTTAVEEMEIED
                     NMIITTKSDTENHGLGMESIKTVVEKYHGKLFLECKNYNFHLMVVIG"
     gene            complement(2337..3059)
                     /locus_tag="PLU43_03060"
     CDS             complement(2337..3059)
                     /locus_tag="PLU43_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO27419.1"
                     /translation="MIKIAVCDDEKNIADNICGRIRTYAARNVLDIEIDAFESAEALF
                     EDNRQFDVYFMDIQMQELDGMSAAAMLRKKNRKAVIVFISAYIQYAAKGYQVNAVRYI
                     LKSQMDEEFDDCMDAVMKSLKKEEVLEIKQGTKRVLLDTADIAYIENEKRKVVIHMLN
                     DGMLTYEIYAKITELAEQLHEMGFIYCHAAFLVNAKAIVGIKQNEFILSNGIHIPISR
                     QKYAQAQKEYYFRKMPSHVFHH"
BASE COUNT         1008 a          799 c          549 g         1106 t
ORIGIN      
        1 atttatcaat gctttgaggg tggtttataa agtactgacg tagaatctca gtatatgtga
       61 agtttttgtg tttcgtcaag aacagcctgt ttgatctgtc acaacctttc gttgttatcc
      121 cctgttttct tcctgtttcc ttgactcttt tccatattct gttatacgat agcagtaaat
      181 tgatactctt tgaaggagaa cccttatcat gatgtactta cacttttgca cagattgcag
      241 acagacctat attttaagcg gtcatcaaca ggaatgtgta aaatgcggcg gcactctcgt
      301 tgaattaaag ctttcctatc aagactatgt tgattattca ccggcccagc gcagacaatt
      361 gatcctggaa ctggaagacc ctgaagaact tgcacgtcaa aaaagacatt accgcttttc
      421 caagcagaca aaacgataca aggaatggat gaaacggaaa gaagacattt cccgctgaat
      481 gaaaatgcct tcttttcgtt tagcctacaa gcatattata ttttctgtcc aaatgtattt
      541 tatcggcaat ctgaaccata tactctgagt tacatggttt ttttctgcta ctcatgatcg
      601 aatacccgaa aatcctctca aatacttccg gattcccctg ttcccacgaa atgccgaccg
      661 cagtacggat cgcccgttct accctgtccg ttgaagaatt gtgtgctttc gcaatatcat
      721 aatacagaat ctttgtaatg gagctcgcaa tttccatatc atgaatcagc ataatgatcg
      781 cttcacgcaa ataccggtaa ccgaggagtt ttgccggtac tcccagttcc cttagcactt
      841 ctgatacata cagttccagt tttacttctt ccttcatgca aatccccctt atatatttct
      901 ttcggtcaca aaccaatgta ccgatatact tattttaaca tttattacca tgtatgcaaa
      961 gggattttca ataattttct aaaattatcc gattacgacc atcagatgaa agttatagtt
     1021 cttgcattcc aaaaacagct ttccgtgata tttctctaca acagtcttta tactttccat
     1081 tccaagacca tgattttccg tatccgactt tgtcgtaatg atcatattat cttcaatttc
     1141 catttcttct accgcagttg tattctggat tgagataatc agctcctgct cttcatttga
     1201 gatctgtatc tcaataatac ggggcgtccc gcccggcagc ttatcgcagg cctctattgc
     1261 attattcaat gcattattca gtattgcagc catatcggac acattcatgc gcagtgttcc
     1321 aagatcctga atattgaaat tcatcgtgat ctttttggaa acggcaacaa tgaacatctg
     1381 gttcagtact gcatctatta caatattatt tgtatgaaca ggcagcacat tcttctctac
     1441 cgcgttggaa atttcggaaa ggtattcttt tgctttatca tattcctcat tttttaaaag
     1501 cgtattcaca atggaaagct gattctcaaa cccatgcgcg atctgcctga tctttttatg
     1561 cgtctgctgc aaagactcca tatattcgat ctcggaccgc atctggttat caagtacttt
     1621 ggaacggctc atttcatcat aatactctcc cattcgatac ataataaaat aaagcatgct
     1681 tcctaaaaat acaagcagca ggatcccgta aatatcccat tccgtaatat ctttcctgat
     1741 cagatttttc ataaagctcg tgtacgcgtt cagcatgatc gcgatcggca caagaataca
     1801 gagcacccgg tcgatcttat acatagcctt gatcttattt cttccgacaa agcatttcag
     1861 cattttgatc agcagcatct gagtaagctt ggaagtcagg gccatataat ataaatacac
     1921 gtcattccca gcaaacgttc cgatatcctc atccagcaaa tgactcatga tctgcagatt
     1981 cacaagatca aataaaagac agaccaggga aacagctatc actgtcagta ttttctgcca
     2041 gactttccct tcgtataaaa taaataccat accgaaagca aataaaagaa tcagaatact
     2101 tttttcatag cttttagaag cagcctcatc cgctatgatc atccataaaa aaagcagcat
     2161 gaccgcaagt atatagtaac tctgttttat tttttttctg tataacgcaa tatcaaaaga
     2221 atccctgatc gccagagatt ccatcagcgt ataaaagtaa gcgcataaaa cccatgggaa
     2281 ttcacctaaa aagcctgctt ccattttatc ctcccataaa aatgcatgtt ccgtaattaa
     2341 tgatggaata catgtgaagg cattttcctg aaataatatt ctttttgtgc ctgtgcatat
     2401 ttctgtctgc tgatcgggat atgtatccca ttgcttaaaa tgaattcatt ttgtttgatt
     2461 ccgacgatcg ctttggcatt tacgagaaat gcggcatgac agtaaataaa tcccatctca
     2521 tgcaattgtt ccgcaagttc cgtgatcttt gcataaattt cataagtcag catgccgtca
     2581 ttaagcatat gaatgacaac ttttcttttt tcattttcaa tataggcaat atccgcagta
     2641 tccagcagca cgcgcttcgt tccctgcttg atctccaata cctcttcttt tttcaagctt
     2701 ttcataaccg catccataca atcgtcaaat tcttcatcca tttgtgattt caagatatat
     2761 ctgacagcat tgacctgata gcccttggca gcatactgaa tatatgccga tataaataca
     2821 atcactgctt ttctgttctt tttgcgcagc atggcggcag cgctcattcc atccagctcc
     2881 tgcatctgaa tatccataaa ataaacatca aactgcctgt tatcttcaaa cagcgcttcc
     2941 gcgctttcaa aagcatctat ttcaatatcc agcacatttc ttgccgcata cgtccggatc
     3001 ctgccgcata tattatccgc tatatttttt tcatcatcac acactgctat ttttatcaca
     3061 acgatcaccc tctgttattt tctcataatt accgctgtgt ttgcaagaaa attttatatt
     3121 acacatgaaa caacctgtgg cggataaacc acaggttgtt caaagaaata aacacaggtt
     3181 gttgagattt gaaatctctg tttattttat accatatcag tcataggcaa tacaaccgac
     3241 ttgtaacaaa tcgcacgata attgtaataa ctggcatcga ttccggcatg tttacaacaa
     3301 attcttgtac catatgcaaa cccttctgaa agcttcctgc agtcaatagc aaaaacgata
     3361 taacaaatat tgcccgaatg ttcccgcata ctgcaggtca aaggcactta aatcccaata
     3421 aggctcctct ttggaaccgg ctgtttcaat atcatacagg tt
//
LOCUS       DAOSYE010000125         3462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19367_length_3462_cov_3.082184, whole genome shotgun sequence.
ACCESSION   DAOSYE010000125 DAOSYE010000000
VERSION     DAOSYE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19367_length_3462_cov_3.082184"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLU43_03065"
     CDS             complement(<1..201)
                     /locus_tag="PLU43_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HOO27420.1"
                     /translation="MAKGHRSQIKRERNANKDTRPSAKLSYARVSVQKACFVLDAIRG
                     KDVASALALLTYNPRYASSIIKK"
     gene            complement(233..514)
                     /gene="rpsS"
                     /locus_tag="PLU43_03070"
     CDS             complement(233..514)
                     /gene="rpsS"
                     /locus_tag="PLU43_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO27421.1"
                     /translation="MSRSLKKGPFADASLLKKVDAMNAENKKQVIKTWSRRSTIFPSF
                     VGHTIAVHDGRKHVPVYVTEDMVGHKLGEFVATRTYRGHGKDEKKSKVR"
     gene            complement(540..1382)
                     /gene="rplB"
                     /locus_tag="PLU43_03075"
     CDS             complement(540..1382)
                     /gene="rplB"
                     /locus_tag="PLU43_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO27422.1"
                     /translation="MGIKTYNPYTPSRRQMTGSDFSEITKTTPEKALCTSLKKNAGRN
                     NQGKITVRHRGGGSRRKYRMIDFKRKKDGIPAKVIGIEYDPNRTANIALICYADGEKA
                     YILAPEGLKDGMKIMNGPEAEVRIGNCLPLSAIPVGTQVHNIELYPGKGGQMVRSAGN
                     SAQLMAKEGKYATLRLPSGEMRMVPLNARASIGVVGNGDHSLINIGKAGRKRNMGIRP
                     TVRGSVMNPNDHPHGGGEGKTGIGRPGPSTPWGKPALGLKTRKKNKKSNKLIVRRRDG
                     KTIK"
     gene            complement(1463..1762)
                     /gene="rplW"
                     /locus_tag="PLU43_03080"
     CDS             complement(1463..1762)
                     /gene="rplW"
                     /locus_tag="PLU43_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO27423.1"
                     /translation="MANIQYYDVILKPVVTEKSMEGMGDKKYTFLVHPEANKTQIKEA
                     VEKMFDGAKVRSVNTMNLDGKNKRRGTVTGKTAKTKKAIVQLTEDSKEIEIFSGL"
     gene            complement(1762..2382)
                     /gene="rplD"
                     /locus_tag="PLU43_03085"
     CDS             complement(1762..2382)
                     /gene="rplD"
                     /locus_tag="PLU43_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO27424.1"
                     /translation="MANVSVYNMEGKEVGKMDLSDAVFGVKVNEHLVHMAVVQQLANN
                     RQGTQKAKTRSEVSGGGRKPWRQKGTGHARQGSTRSPQWKGGGVVFAPVPRDYSFKLN
                     KKEKRFALKSALTSRVQENKFLVLDELKLEEIKTKKFQEVLNNLNVSKAIVILDSDDK
                     NVILSARNIPNVITAFTNTINVYDIMKYNTVIATKGAVAKIEEVYA"
     gene            complement(2411..3094)
                     /gene="rplC"
                     /locus_tag="PLU43_03090"
     CDS             complement(2411..3094)
                     /gene="rplC"
                     /locus_tag="PLU43_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO27425.1"
                     /translation="MKKAILATKVGMTQIFSENGVLVPVTVLTAGPCVVTQIKTVEND
                     GYNAVQVGFKDKKDKVINKDKSGKKEIIHRNGVTKAQKGHFDKAGVSSKRFIREFDFD
                     NAAEYALAQEIRADIFEVGDKVDATAISKGKGFQGAIKRHGQSRGPMKHGSKFHRHAG
                     SNGACSSPSRVFKGKKMPGQMGAKRITVQNLEVMRADGENNLLLIKGAIPGPRKSLVT
                     IKETVKTIL"
     gene            complement(3156..>3462)
                     /gene="rpsJ"
                     /locus_tag="PLU43_03095"
     CDS             complement(3156..>3462)
                     /gene="rpsJ"
                     /locus_tag="PLU43_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO27426.1"
                     /translation="VMRITLKAYDHQLVDASAKKIIETVKKNGSQVSGPVPLPTKKEV
                     VTILRAVHKYKDSREQFEQRTHKRLIDIITPTQKTVDSLSRLEMPAGVYIDIKMKNK"
BASE COUNT          758 a          861 c          635 g         1208 t
ORIGIN      
        1 cttcttgata atgcttgatg catatctggg attgtatgtc aaaagtgcca atgctgacgc
       61 tacatctttt cctctgatgg catctaatac gaaacatgct ttctgaacag atactctcgc
      121 ataagataac tttgccgagg gtcttgtatc cttgttcgca tttctttctc tttttatttg
      181 ggatctatgt ccttttgcca tggatgaacc ctcctttcaa aatatatgaa aattatctaa
      241 cttttgactt cttctcgtct tttccgtgtc ctctgtaagt tcttgttgca acgaactcac
      301 cgagcttgtg gcctaccata tcctctgtaa catatacagg cacatgtttt cttccgtcgt
      361 gaaccgcgat cgtatgtccg acaaatgacg ggaaaatcgt agaacggcga gaccatgtct
      421 ttattacctg ttttttgttt tctgcgttca tggcatcaac ttttttcagt aaactcgcgt
      481 cagcgaatgg gcctttttta agcgatcttg acattttatt tcctcctatt atcaaccact
      541 tacttgattg tctttccgtc tctgcgtctt acaatcagct tattggattt cttgttcttc
      601 tttcttgttt tcagaccaag tgcaggcttg ccccatggtg tagacggtcc cggacggccg
      661 ataccggtct tgccttcacc gccgccgtgc ggatgatcat tcggattcat aacagaaccg
      721 cggacagtag gtctgatacc catattgcgc ttacgtcctg ccttaccgat attgatcagg
      781 ctgtggtcac cgtttcctac aacaccgata gaagctcttg cattaagcgg aaccattctc
      841 atttcgcccg aaggaagtct gagtgttgca tacttgcctt ccttagccat aagctgtgcg
      901 ctgttgccgg cagagcgaac catctgaccg cctttgccgg gatataattc aatgttgtgt
      961 acctgtgtac caacagggat tgcagaaaga ggcagacaat taccgattct tacttcagct
     1021 tcaggtccgt tcatgatctt catgccgtcc ttaagtcctt ccggtgcaag aatataggcc
     1081 ttctcgccgt ccgcataaca gatcagcgca atatttgccg tcctgttcgg atcatattca
     1141 ataccgataa cctttgcagg tatcccgtct ttctttctct taaaatcaat cattctatat
     1201 tttcttctgc tgccgcctcc gcggtgtctt acagtgatct taccctgatt attacgaccc
     1261 gcattctttt tcagcgaagt acagagagcc ttttccggtg ttgttttcgt aatttccgaa
     1321 aaatcggaac ccgtcatctg ccttctggaa ggggtatatg ggttataggt tttaattccc
     1381 attgttattt ctcctttaca atttattatc acacttattt tgtgtatagt atggaggaag
     1441 gaatgccttc ccgcgtctta ttttacaatc ctgagaaaat ctcaatttcc ttgctgtctt
     1501 cggtcagctg aacgatcgct tttttcgtct ttgcagtttt acccgtaaca gttccccgtc
     1561 ttttattctt tccgtcaaga ttcattgtat tgacgcttct tacctttgcg ccgtcaaaca
     1621 ttttttcaac agcttctttg atctgtgtct tatttgcttc cggatgtaca agaaacgtat
     1681 acttcttatc tcccatacct tccatgctct tttcagtaac aaccggtttg aggatcacgt
     1741 cataatactg aatatttgcc attatgcata cacctcctcg atcttagcta cggcaccctt
     1801 cgtcgcaatc accgtattat acttcataat atcatataca ttgatcgtat ttgtgaaagc
     1861 agtgattaca ttcgggatat ttctggcaga aagaattaca ttcttatcgt cagaatccaa
     1921 aatcacaatt gcctttgata cgttcaggtt attaagcact tcctgaaatt tctttgtctt
     1981 gatctcctca agcttcaact catcaagtac taaaaacttg ttttcctgca ctcttgaagt
     2041 aagtgcggat tttaatgcaa atctcttttc tttcttgttt aatttgaaag aataatcacg
     2101 cggaaccggt gcgaatacta ccccgccgcc tttccactgg ggagatcttg tcgaaccctg
     2161 ccttgcatgg ccggttcctt tctgtctcca tggctttctg ccgccgccgg aaacttccga
     2221 gcgcgtcttt gctttctgtg ttccctgacg attattagca agctgctgaa caactgccat
     2281 atgcactaaa tgttcattga cctttacgcc gaatacggca tcactaagat ccatcttgcc
     2341 gacttctttg ccttccatat tgtaaacaga tacgtttgcc atctgtgtgt tcctcctttc
     2401 catataaaaa ctaaagaata gtttttaccg tttcttttat tgtcaccaat gatttccttg
     2461 gtcctggtat tgcgcccttg atcagcagca gattgttttc cccgtccgct ctcattactt
     2521 caaggttctg gactgtgatc cgctttgctc ccatttgtcc gggcatcttc ttgcctttga
     2581 acacacggct tggactggaa cacgcaccat tggaacctgc atggcgatgg aacttggaac
     2641 catgtttcat tggtcctctt gactgtccgt gtctcttgat cgcaccctgg aatccttttc
     2701 cttttgaaat cgcagtcgca tccactttat caccgacttc aaaaatatcg gctctgattt
     2761 cctgtgccag ggcatactct gccgcattgt caaaatcaaa ttccctgata aacctctttg
     2821 aacttacgcc tgctttatca aaatgtccct tctgtgcttt tgtaacaccg tttctgtgaa
     2881 tgatttcttt tttcccggat ttgtccttat tgatgacttt atccttctta tccttgaaac
     2941 caacctgtac cgcattatat ccgtcgttct caacggtctt gatctgtgtt acgacacagg
     3001 gaccggctgt tagtacggta accggtacca aaacgccatt ttcactgaag atctgtgtca
     3061 ttccgacttt tgttgctaaa attgcttttt tcatgcttgc ctcctgtttt cgctacagcg
     3121 gaacgcctgt cctgacgttc atactagtag gggtcttatt tgtttttcat tttgatatca
     3181 atataaacac cagccggcat ttccagtctt gacagggaat ctaccgtttt ctgggtcggt
     3241 gtgatgatat caatgagtct cttatgagtt ctctgttcga actgctctct ggaatctttg
     3301 tacttatgaa ccgctcttaa aattgtaaca acttcctttt tcgtaggaag tggaaccggg
     3361 ccgctcacct gtgatccgtt tttctttaca gtttcgatga ttttttttgc cgatgcatct
     3421 actaattgat gatcgtacgc ttttaatgtg attctcatta cc
//
LOCUS       DAOSYE010000126         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19430_length_3456_cov_2.893561, whole genome shotgun sequence.
ACCESSION   DAOSYE010000126 DAOSYE010000000
VERSION     DAOSYE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19430_length_3456_cov_2.893561"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLU43_03100"
     CDS             <1..631
                     /locus_tag="PLU43_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27427.1"
                     /translation="VGRGEIVGLVGESGCGKSTLAKAILHMVKDTEGEVTHYSKMPQM
                     VFQDPYGSLNPSKKIGWILEEPLKIKGGFSDVERSRRVCAMLEKVGLDEKFSERYPHE
                     LSGGQRQRICIALALMLEPKLVIADEPVSALDVTVQAQILQLLLRLNKELGVALLFIS
                     HDLKVVYQLCSRILVMKDGRIIEQGSDREIYYKPKQAYTRELLNSAGIR"
     gene            781..1209
                     /locus_tag="PLU43_03105"
     CDS             781..1209
                     /locus_tag="PLU43_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27428.1"
                     /translation="MAINGLSQYDLFSSYNSINSADRIPKAEPVGIEEQSKNAQTDTS
                     GIPEQESAPQSLSLDLDSIRSRGNASLEDISLSLQSSSEFDMKGRDSNLGSLDMEKAV
                     SDMQKDQALMQYQYFVGDSSNVISSGEDGTVVQKSSDEML"
     gene            1334..2278
                     /locus_tag="PLU43_03110"
     CDS             1334..2278
                     /locus_tag="PLU43_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO27429.1"
                     /translation="MKLTTEKKREVLAGFGLLLIAFIWGFAFVVVKNSLDYIPAVYML
                     AFRFTIAGVALAVVFFKKLKKLNRYLLLHGGILGLLLFTAYLFQTIGCKYTTAGKNAF
                     LTTIYVILVPFFHWIISKKRPDAYCMSAAVLAVIGIGLLSLQGDLTVNIGDILTIVCG
                     IFYALHVIFIDRYTKADDPVILAVLQVVFAALFSWLTAPFIDGGFPQEAFRIDIVIGM
                     LYLGVFSTMIGFLLQTVCQKYTHPSTSALLMSTEAVFGALCAAIFLREVMSGRMITGC
                     LLMFAAIVLAETKPGRKEALRKRRLADPELDNKKIVKE"
     gene            2283..2798
                     /locus_tag="PLU43_03115"
     CDS             2283..2798
                     /locus_tag="PLU43_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOO27430.1"
                     /translation="MLDCFFPDEYEDSVYEIDFDRYYAEGYRGIIFDIDNTLVPHGAP
                     AEERTIALFEGLKQTGYALLLLSNNKEPRVKSFNDKVHAAYIFKAHKPGKDGYIRAMR
                     ELGCNTENTLFIGDQLFTDVWGAKRTGIRNILVKPIHPKEEIQIVLKRKLEKIVLYCY
                     YKKFMRQAGHK"
     gene            2817..>3456
                     /locus_tag="PLU43_03120"
     CDS             2817..>3456
                     /locus_tag="PLU43_03120"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO27431.1"
                     /translation="MEINGETRILGIIGKPIRHTMSPVIHNMISEAQGRNMVYVPFEV
                     ENDAAAAVKGAYALGIIGMNVTVPYKTAVTESLLEIEELAERIGAVNTLVRTETGFKG
                     YNTDISGLERELADENVSLEGRHVLLLGAGGAARAAAFLCVKLRAASVTILNRTADKA
                     EAIAKDVCAYAGEACSDMHTVVKAMPLNQYGTLTGSGYIALQSTKLGMYPKTE"
BASE COUNT          973 a          645 c          922 g          916 t
ORIGIN      
        1 tgtcgggcgc ggcgaaatcg tcggcctggt cggcgaaagc ggctgcggaa aatcgacgct
       61 tgcgaaagcg atccttcata tggtaaagga tacggaagga gaagtaacgc actattccaa
      121 aatgccgcag atggtatttc aggatccgta cggttctttg aatccgtcca aaaaaatagg
      181 ctggatattg gaggaacccc ttaagatcaa aggcggtttt tcagacgtgg agcgcagcag
      241 gagagtgtgc gccatgcttg aaaaggtagg tcttgatgaa aagttttcgg aaagatatcc
      301 gcatgaactg tccggaggac agaggcagag gatctgtatt gcgcttgccc tgatgcttga
      361 accgaaactt gtcattgcgg atgagccggt ttccgcgttg gatgtaacgg tacaggcaca
      421 gatcctgcag ctgcttctgc gtttgaataa agagcttgga gttgcactgc tttttatatc
      481 gcatgacctg aaagtggtct atcagctttg cagccgcatt cttgtgatga aagacggcag
      541 gatcattgaa caggggagtg acagggagat ttattataaa ccaaaacagg cgtatacacg
      601 ggaacttctg aactcggcag gaatcaggta aacgtataag tttttgaaaa aaagtattaa
      661 ttttataacg aaaaatgacg atataatgat tgcaaacata caacagaatg cgttataatc
      721 taaatgtaaa cttattatgt caatctaaga gagaacaagc atgtacaagg aagtgataga
      781 atggcaatta atggattaag tcaatatgat ttgttttcat cgtataacag tataaattct
      841 gcagaccgga tcccaaaggc tgagccggtt ggaattgaag agcagagcaa aaatgcgcag
      901 actgatacca gcggtattcc cgaacaggaa tctgcccccc agtcgttatc cttagacctt
      961 gattctatca gatcccgcgg caatgccagt cttgaagata tatcattaag cctgcagtct
     1021 tccagtgaat ttgatatgaa gggcagggac agcaatttag ggtcccttga tatggaaaaa
     1081 gcggtttcgg atatgcagaa ggatcaggcg ctcatgcagt atcagtattt tgtaggtgat
     1141 tcttcaaacg tgatcagcag cggggaagac ggaacagtcg ttcagaaatc ttcagatgaa
     1201 atgttataaa tctcttgttt ttcatgggca ggagatttat tttttttgtt tttttttggc
     1261 tgttgaagtg tgaatcgtac tttgctataa tggacaaata tgatattgtg taaaacatag
     1321 gatggtaaca gtgatgaaac ttacaacaga aaaaaagaga gaagtactgg ccggattcgg
     1381 tctgctgctg attgcattta tatgggggtt tgcttttgta gtcgtcaaaa attcattaga
     1441 ctatatacct gccgtttata tgctggcatt tcggtttacg atagcaggag ttgcgcttgc
     1501 cgttgttttc tttaaaaaat taaaaaagct gaataggtat cttttattgc acggtggaat
     1561 actcggactg cttcttttta cagcgtatct gttccagacg atcggatgca aatatacgac
     1621 tgcagggaaa aatgcatttc tgacgacgat ctatgtgatc cttgtgccgt tctttcattg
     1681 gatcatcagt aaaaaaagac cggatgcata ttgcatgagt gcggccgtgc ttgcggtgat
     1741 cggtattgga ctgctgtcat tgcaggggga cctgacagtg aatatcggag atattctgac
     1801 gatcgtgtgc ggtattttct atgcacttca tgtaatattt atagaccggt atacaaaggc
     1861 agatgatccg gttattctgg cggttctgca ggtcgtgttt gccgcattgt tttcatggct
     1921 tacagcgcct tttatagacg gtggatttcc gcaggaggcc ttccgtatcg atattgtgat
     1981 cggcatgctg tatcttggtg tgttcagtac aatgatcggg ttcctgctgc agaccgtatg
     2041 ccagaaatat acgcatcctt ccacgtccgc actgctgatg tctacggaag ccgtattcgg
     2101 tgctctgtgt gctgcgattt ttttgcggga agtcatgagc ggacgaatga taaccggctg
     2161 tctgctgatg tttgcggcaa tcgtactggc tgagacaaag cccggcagga aagaagcttt
     2221 gcggaagaga cggcttgcgg atcctgaact ggacaacaaa aagattgtga aggagtagca
     2281 gaatgctgga ttgttttttt ccggatgaat atgaagattc ggtttatgaa attgattttg
     2341 accgttacta cgcggaagga taccgtggaa ttatttttga tatcgacaat acgcttgttc
     2401 cgcacggagc gccggctgaa gagcgtacga tcgctctgtt tgaagggctg aaacagaccg
     2461 gctatgcgct gctgctgctc tccaataata aggagccccg tgtaaaatca tttaatgaca
     2521 aggtacatgc ggcttatatt tttaaggcgc acaaaccggg aaaagacggc tatatcagag
     2581 caatgcgtga gcttggctgc aatacggaaa atacgctgtt tatcggagac cagctgttta
     2641 cggatgtctg gggagccaaa cggacaggga tccggaatat tctcgtaaag ccgatccatc
     2701 cgaaagaaga gatccagatc gtattgaaac gaaagctgga aaagatcgta ttgtactgct
     2761 attataaaaa gtttatgcgc caggcagggc ataaataggg gagggcagat ataccaatgg
     2821 agatcaacgg agaaacgagg atacttggca ttatcggaaa accgatcagg catacgatgt
     2881 cgccggtcat tcataatatg atttcagagg cacagggacg aaatatggtt tatgtcccgt
     2941 ttgaagtgga gaacgatgcg gcagctgccg taaaaggtgc ttatgcactc ggcattatcg
     3001 gaatgaatgt gacggttccg tataaaactg ctgtgacgga aagtctttta gaaattgaag
     3061 aactggcgga aagaataggt gccgtcaata cgcttgtcag aacagaaact ggattcaagg
     3121 ggtataatac ggatatcagc gggctggaga gagagcttgc ggacgagaat gtttctttgg
     3181 agggcaggca cgttctgctg ctcggcgcag gcggggcagc acgcgcagca gcttttttat
     3241 gtgtaaagct gcgggcagct tccgttacaa tactgaacag aacagctgat aaggcggaag
     3301 cgatcgcaaa ggatgtctgt gcatatgccg gtgaggcatg ttccgatatg catacagttg
     3361 taaaagcaat gccgctgaat cagtatggga cactgaccgg cagcggatat attgcacttc
     3421 agagcaccaa actcggtatg tatccaaaaa cagagg
//
LOCUS       DAOSYE010000127         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19452_length_3454_cov_2.564578, whole genome shotgun sequence.
ACCESSION   DAOSYE010000127 DAOSYE010000000
VERSION     DAOSYE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19452_length_3454_cov_2.564578"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..529
                     /locus_tag="PLU43_03125"
     CDS             113..529
                     /locus_tag="PLU43_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOO27432.1"
                     /translation="MFGIFGKFKRKRRIDAEAEKGAYQAWTFGPALLNDDGTAITDFS
                     GISKRMENKNPRSAIGYYEPGHYCLVLVDGRSKGYSCGMTMEELSELFEELGCSTAYN
                     LDGGKSSVMTFQDEIVNQPYEGGRESSDCILIKEAE"
     gene            529..741
                     /locus_tag="PLU43_03130"
     CDS             529..741
                     /locus_tag="PLU43_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27433.1"
                     /translation="MKILKTIYGLFPHMTFILSIMFVTFLILDQYNPMINFVDNSISM
                     VLLLIMSILTLVNSIVIIIFMHQNNF"
     gene            866..1408
                     /locus_tag="PLU43_03135"
     CDS             866..1408
                     /locus_tag="PLU43_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOO27434.1"
                     /translation="MKKIDISQMAVIGVMTAVLCVLAPITLPIGLVPLSLANFVIFIS
                     VYVLGIWKGTVSCILYLLIGLIGVPVFSGFSSGPAKLLGPTGGYLIGYVFMALIAGFF
                     IDRFKCRKLPSLLGMLLGTLVLYLLGTVWLAYQAGMTFMAALWAGVIPFIAGDLAKII
                     IALFLGPILRERLQKAGLYK"
     gene            1555..2280
                     /locus_tag="PLU43_03140"
     CDS             1555..2280
                     /locus_tag="PLU43_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO27435.1"
                     /translation="MTEQTGVRINKYIADAGVCSRREADRLVEQGRVSADGRAAEKGT
                     RILPGMCVTVDGKEIRQSEKKVYLAFHKPKGIVCTSEKREKDNVIDFIGYPARITYCG
                     RLDKDSEGLLLLTNDGSIINQMMRAANYHEKEYVVTIDRPVTKEFLKQMRTGVYLDGL
                     DTVTRPCAVKKTAENTFHMILTQGLNRQIRRMCEALGCRVVKLCRIRVMNILLGDLPA
                     GTYRELSETELMELRKEIMSERK"
     gene            2283..>3454
                     /locus_tag="PLU43_03145"
     CDS             2283..>3454
                     /locus_tag="PLU43_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO27436.1"
                     /translation="MGEIERMKELVSLLNSASKAYYQDSGEIMSNLEYDALYDELVKL
                     EEQTGTVLAGSPTATVGYEAVGELPKEPHESPMLSLDKTKEREALRDWLKGREALLSW
                     KLDGLTIVLTYRDGLLYKAVTRGNGEIGEIITNNAKTFKNLPFTIPYKGELILRGEAV
                     ISYADFKKINEEIPETEAKYKNPRNLCSGSVRQLNNEITAKRNVRFYAFSLVKADGVD
                     FQNARSRQMEFLKQQGFETVEYYLVTEADILARIQFFADSVTQNAIPSDGLVLTYEDL
                     AYGQSLGRTSKFPRDSIAFKWADESRETTLLGIEWSPSRTGLINPVALFEPVELEGTT
                     VSRASVHNISIVKSLALGIGDRITVYKANMIIPQIAENLTRSGELPIPDRFSAICG"
BASE COUNT         1000 a          621 c          929 g          904 t
ORIGIN      
        1 ttatcggctt aactgctatt tatgcatttg catggtgcct gttcaagatg aaacgaaatc
       61 cgttcaaacc gatttcgata gaagaagcgc tggttgagta aggtaagtta gcgtgttcgg
      121 gatatttgga aaattcaaac ggaaaagaag aattgacgca gaggcagaaa aaggagcgta
      181 tcaggcatgg acattcggac ctgctctttt aaatgacgat ggaacggcaa ttacggattt
      241 cagcggtata tcaaaacgga tggagaataa aaatccaaga agcgcgatcg gatactatga
      301 accgggacat tactgtcttg tgcttgtgga tgggcgttcc aaaggatact cttgcggaat
      361 gacaatggaa gagctgtcgg aattatttga agagcttgga tgcagcacag cgtataatct
      421 ggacggcgga aaatcttcgg taatgacatt tcaggatgag atcgtaaatc agccgtatga
      481 aggcggaaga gaaagcagtg actgtatatt gatcaaggag gccgagtaat gaaaatactg
      541 aaaactattt acggattatt tccgcatatg acatttattt tatcgatcat gtttgttaca
      601 tttttgattt tggatcaata taatccaatg atcaattttg ttgataactc gatctcaatg
      661 gtattattgc ttatcatgag tattttgaca cttgtgaatt ctattgtgat catcattttt
      721 atgcatcaga ataatttcta acgattggga ggtcggtttt ttcatggtag atgcctattg
      781 tcacccaata tataaaaata tgttgacaat agaaaagaaa aaatgtaaaa tgaagaaaca
      841 gaagacatta aggaggattt ctattatgaa aaagatcgat atttcgcaga tggcggttat
      901 cggagtcatg acggcagttt tgtgtgtgct tgcgccaatc acgcttccga ttggacttgt
      961 accgctgtcg cttgccaatt ttgttatttt tatcagtgtt tacgtacttg gaatatggaa
     1021 gggtacagtc agctgtatac tttatctgct gatcggactg atcggggttc ctgtattttc
     1081 cggattttcg agcggacctg cgaagctgct tggtccgaca ggcgggtatt tgatcggcta
     1141 tgtgtttatg gctctgatcg cgggcttttt tattgaccgt ttcaaatgca gaaagcttcc
     1201 gtcattgctt ggcatgctgc ttggaacgct cgttctttat ctgcttggga ccgtatggct
     1261 tgcctatcag gcagggatga cattcatggc agcattgtgg gccggtgtga taccatttat
     1321 tgcaggcgat ctggccaaaa taataattgc tctgttttta ggtccgatct taagagagag
     1381 gctgcagaaa gccggactgt ataagtagaa aatagtataa tgaaagagta agatccattc
     1441 gctgagtttg ccgcgaatgg attctttttt tgttccatat caattaaaaa tgaggtatac
     1501 tgtatttaat tgcagcaaaa tcaggaactt gttttagggg agcagaaaag agtcatgaca
     1561 gaacagacag gagtcaggat caataaatat attgcggatg ccggcgtgtg ttcgcgcagg
     1621 gaggcagacc gccttgtgga gcagggacgt gtatctgcag acggcagggc agctgaaaaa
     1681 ggaacgcgta ttctgccggg catgtgcgta acagttgacg gaaaagagat cagacagagt
     1741 gaaaagaagg tctatcttgc atttcataag ccaaaaggga tcgtttgtac aagtgaaaaa
     1801 cgggaaaagg ataatgtgat cgactttatc gggtatcctg cccgtattac atattgcggc
     1861 cgtctggata aggattccga gggactgctt cttttgacga atgacggcag tatcatcaat
     1921 cagatgatgc gggcggcgaa ttaccatgaa aaagaatatg ttgttacaat agaccggccg
     1981 gtaacaaagg aatttctaaa acagatgcgg acgggtgttt atctggacgg actggatacg
     2041 gtgacaagac cctgtgcagt gaagaagaca gcagaaaaca cgtttcatat gatcctgaca
     2101 cagggactta accggcagat ccgcagaatg tgtgaggcgc ttggctgtcg tgttgtaaag
     2161 ctgtgccgta tccgtgtgat gaatattctg cttggcgatc tgcctgcggg aacttaccgg
     2221 gagctgagcg aaacagagct tatggaatta cgaaaagaaa tcatgtctga gaggaaataa
     2281 tcatgggaga gatagagaga atgaaggagc ttgtgagcct tctgaacagt gcgtccaagg
     2341 cgtattatca ggacagcggg gagatcatga gcaatctgga atatgatgcc ctttatgacg
     2401 agcttgtgaa actggaagag cagacgggaa ctgtgcttgc aggaagtccg acggccactg
     2461 tcggttatga agctgttgga gaactgccaa aggagccaca tgaaagtccg atgctgtcgc
     2521 ttgataagac aaaggaacga gaagctttgc gtgactggct caagggcagg gaagcactgc
     2581 tgtcatggaa gctggacggc ctcacgatcg tgctgacgta cagagatggt ctgctgtata
     2641 aagcggttac acgcggaaac ggggagatcg gggaaattat cacgaataac gcaaagacct
     2701 ttaagaatct gccgtttacg atcccatata agggagaact gatactgcgc ggcgaagcag
     2761 tgatcagcta tgcggatttc aaaaaaataa atgaagagat accggaaacg gaggcaaaat
     2821 ataaaaatcc gagaaatctg tgcagcggtt cggtcagaca gctgaacaat gaaataacgg
     2881 caaaacgaaa tgtgcggttt tatgcgtttt ctcttgtgaa ggcagatggt gtggattttc
     2941 agaatgcaag aagccgccag atggagtttt taaaacagca gggttttgaa acggtcgaat
     3001 attaccttgt aacagaagca gatattcttg cgcgtattca gttttttgct gattccgtta
     3061 cacagaatgc gattccctca gacgggcttg tactgaccta tgaggatctt gcatacgggc
     3121 agtcacttgg acgtacatcc aagttcccgc gggattcgat cgcttttaag tgggcagatg
     3181 agagcaggga aacgacgctt cttggaatcg aatggagtcc gagccgtacc ggtctgatca
     3241 atccggtcgc actatttgag cctgtggagc tggagggaac gaccgtcagc cgtgcaagcg
     3301 tgcataatat cagtatcgta aaatcgcttg cacttggtat cggagacagg atcacggtct
     3361 ataaagccaa tatgatcatt ccgcagatcg cagaaaatct gacaaggagc ggggaactgc
     3421 cgattccgga cagattttct gcgatctgcg gaat
//
LOCUS       DAOSYE010000128         3437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19622_length_3437_cov_2.901833, whole genome shotgun sequence.
ACCESSION   DAOSYE010000128 DAOSYE010000000
VERSION     DAOSYE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19622_length_3437_cov_2.901833"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLU43_03150"
     CDS             <1..826
                     /locus_tag="PLU43_03150"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOO27437.1"
                     /translation="NPDIAKLAEKEKKNFAGCEIQDKKLGIIGLGAIGVRVANVAKHL
                     GMEVYGYDPYISVNAAWNLSRDIKHVLNVDEIYEQCDMITIHVPLMDSTKGKINQDAI
                     SKMKDGVILLNFARDLLANEADVVEAVKSGKVAKYVTDFPNTTTAGVDGCIVIPHLGA
                     STEESEDNCAKMAVKELMNYLENGNINHSVNYPECDMGVCSTCGRVAIMHKNVANMIT
                     KFTATFGDLGINISDMLNKSKGEYAYTMLDVESPVTDAIVEKLRAIDGVIRVRAVK"
     gene            complement(888..1154)
                     /locus_tag="PLU43_03155"
     CDS             complement(888..1154)
                     /locus_tag="PLU43_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27438.1"
                     /translation="MTLEERQFLDKINQYAENVLKDIEPQKTLVSTQIEKLKPVMEML
                     AKENDLPLEEIFIKYMDLQSELSAENEQKFKEQTLSGPDFISGL"
     gene            1321..2562
                     /locus_tag="PLU43_03160"
     CDS             1321..2562
                     /locus_tag="PLU43_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOO27439.1"
                     /translation="MADIRPFRAIRPKQGLESRIAALPYDVYNREEAKKETQKEALSF
                     LKIDRAETQFDDSVDIYADVVYRRAHDTLWEMIKDGELIKEKEACYYLYELTMNGRSQ
                     TGIVACASIDDYTANVIRKHENTRADKEEDRIRHVDALDAQTGPIFLAYRAQNEINEA
                     VTQIKKEPAVYDFVSPDNIRHTVWIISQKDTMELIRKKFSDMREIYIADGHHRCASAV
                     KVGLKRRAEKEKYTGNEEFNYFLSVLFPDEQLLIMDYNRVVKDLNGHSEKAVFSLLRE
                     KGTLENHGAVSYRPKKKGEVGIYFENEWYSFVFDDSLKSADPVEGLDVSILQKQILEP
                     VFGISDPKTDKRIDFVGGIRGLEELEKRVHTDSKIAFAMYPTHIDELFAVADAGRLMP
                     PKSTWFEPKLRSGLFIHEIDR"
     gene            2577..>3437
                     /locus_tag="PLU43_03165"
     CDS             2577..>3437
                     /locus_tag="PLU43_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOO27440.1"
                     /translation="MNKKLYKMMDWAEIETVVYSEEDHPHDFLGAHYVSGGILIQAFF
                     PDVKKVYAIVKMNDVIKEYPMELADEEGFYAVLVKSLTKAKYSYHFKVLMKNNTFIEI
                     EDPYRFPSTVKQSDIEKFNAGIHYTIYDVLGAQVRKINGVAGTSFAVWAPNALRVSVV
                     GNFNHWDGRIHQMRRLGESGIFEIFIPGISDGEIYKYELKLKGGMISLKADPYGFGAE
                     LRPGTASVVRNIKNHCWSDEQWLMDRKTGQSGMRPMIVYEVHLGSFKKPEDTREFYNY
                     RELAPMLAEYV"
BASE COUNT         1068 a          602 c          836 g          931 t
ORIGIN      
        1 gaatccggat attgcaaagc ttgcggaaaa agagaaaaag aattttgcag gctgtgaaat
       61 tcaggataag aaacttggaa ttatcggact cggcgcaatt ggtgttagag tggcaaatgt
      121 ggcaaagcat cttggtatgg aggtttatgg atatgaccct tatatttcgg tcaatgcggc
      181 ctggaacctt tccagagata tcaaacacgt tctgaatgtg gatgaaatat atgaacagtg
      241 cgacatgatt acgatccatg tgcctcttat ggattccaca aagggaaaga tcaatcagga
      301 cgccatttca aaaatgaagg atggtgtgat ccttttgaat tttgcaagag atcttcttgc
      361 aaatgaagca gatgtagttg aggccgtaaa gtccggcaag gttgcaaaat atgtgacgga
      421 ttttccgaat acgacaacag cgggtgtgga cggctgtatc gtcattccgc atttgggcgc
      481 ctccacggaa gagtctgagg acaactgtgc gaaaatggct gtcaaagagc ttatgaatta
      541 tctggaaaat ggcaatatca atcattccgt caattatccg gaatgtgata tgggtgtttg
      601 ttccacctgc ggccgggtgg cgatcatgca taaaaatgtg gcaaacatga tcacgaaatt
      661 cacagctact tttggtgatc ttgggattaa catttcggat atgctgaata aatcaaaggg
      721 tgaatatgca tatacaatgc tggatgtgga atctcctgtg accgatgcga tcgtagaaaa
      781 actaagggcc attgacggtg taatccgcgt aagagccgta aagtaattat acaaactgca
      841 taatatcaaa attcccaccg tctttgcagc gatgggaatt tgttgtttta cagaccgctt
      901 ataaagtcag gaccggagag ggtctgctct ttgaatttct gttcgttttc cgcagataat
      961 tcactctgca gatccatata tttgatgaaa atttcttcaa gcggcagatc attttctttt
     1021 gcgagcattt ccataacagg ctttaatttt tcgatctggg tggaaacaag ggttttctgc
     1081 ggctcaatat ccttcagtac attttcagca tattgattga ttttgtccag aaattgccgt
     1141 tcttctaagg tcatggcaaa gcctcctttc tgcagacaaa agtatagcat gcgcagcagc
     1201 ggctgtcaaa atccagtcct gctattgtga aaataaataa attttggtaa aataatgtag
     1261 ggcatatgtt tttattgaaa agacatgagc agtaacgatg attcggagga gtaaaacaat
     1321 atggcagata tcagaccatt tcgggcaatc agaccaaaac agggattgga aagcagaatt
     1381 gcagcattac catatgacgt atacaacaga gaagaagcca aaaaagaaac ccaaaaagag
     1441 gcattgtctt ttttgaagat cgaccgggca gagacacagt ttgacgattc cgtggatatc
     1501 tatgcggatg ttgtatacag gcgtgcccat gatacactgt gggaaatgat aaaagacggg
     1561 gaactgatca aagaaaaaga ggcatgttat tatttgtatg aactgacgat gaacggaagg
     1621 agccagacag gtatcgttgc ctgtgcttcg attgacgatt atactgccaa tgtcataaga
     1681 aaacatgaaa atacaagggc tgataaagaa gaagaccgta tccgccatgt ggatgccctg
     1741 gatgcgcaga caggaccgat ctttcttgcc tacagagccc aaaatgaaat caatgaggct
     1801 gtcacacaaa tcaaaaaaga accggcagtt tatgattttg tatcaccgga caatattcgc
     1861 catacggtat ggatcatctc ccagaaggat acaatggaac tgattagaaa aaaattttcc
     1921 gatatgcggg aaatctatat cgcagacggt catcatcgtt gcgcctccgc agtaaaagtc
     1981 ggtttgaaaa ggcgggccga aaaggaaaaa tacacgggaa acgaagaatt taattatttt
     2041 ctttccgtat tatttcctga cgaacagctg ctgattatgg attacaaccg tgttgtgaaa
     2101 gatttaaacg gacattctga aaaagctgtt ttttcgcttt tgcgtgagaa agggactttg
     2161 gaaaatcatg gagccgtgtc gtacagaccg aaaaaaaagg gagaagttgg tatttatttt
     2221 gaaaatgaat ggtattcctt tgtgtttgat gatagtttaa aatcggctga tcctgtggaa
     2281 ggactggatg tatccatttt acagaaacag atactggaac ctgtatttgg gatttctgat
     2341 ccgaaaaccg ataaaagaat tgattttgtc ggcgggattc gtggacttga agaattggag
     2401 aaacgggtac atacagacag caaaattgca tttgccatgt atccgaccca tatagatgaa
     2461 ttatttgccg tggccgacgc aggcagactg atgccgccga aatccacatg gttcgagccg
     2521 aagctgcgaa gcggtttatt tattcatgaa attgacagat aggagcgagc gtgtacatga
     2581 acaaaaaatt gtataaaatg atggactggg cagaaataga aacagtcgtt tattcggaag
     2641 aagatcaccc gcatgatttt cttggtgccc attatgtaag cggcggaatt ttgatacagg
     2701 cattttttcc ggatgtgaaa aaagtgtatg ccattgtcaa aatgaatgat gtaatcaagg
     2761 aataccctat ggagcttgca gatgaggagg gattttatgc ggtacttgtt aaatccctta
     2821 cgaaagcaaa gtattcttat cactttaaag ttctgatgaa gaataatact tttatcgaaa
     2881 tagaagatcc ttatcgattt ccaagtactg tcaagcagtc tgatatcgaa aaatttaatg
     2941 ccgggatcca ttatacgatc tatgacgttc ttggcgcgca ggtcagaaaa atcaacggcg
     3001 ttgcggggac ctcttttgca gtgtgggcac cgaacgcatt aagggtcagc gttgttggaa
     3061 atttcaatca ttgggacggc cgtattcatc aaatgaggag acttggcgag agcggtattt
     3121 ttgagatctt tattccaggc atcagtgatg gggagattta taagtatgaa ttaaaattaa
     3181 agggcggcat gatcagctta aaggctgatc cgtatggttt tggcgcggag ctgcgtcctg
     3241 gtacggcttc ggtcgtacga aacataaaaa atcattgctg gtcggatgaa cagtggctta
     3301 tggacaggaa aaccggacaa agcggtatgc ggccgatgat cgtttatgaa gtacatcttg
     3361 gttcgttcaa aaaaccggaa gatacaagag aattttataa ttacagggag cttgccccga
     3421 tgcttgccga atatgta
//
LOCUS       DAOSYE010000129         3432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19669_length_3432_cov_3.350607, whole genome shotgun sequence.
ACCESSION   DAOSYE010000129 DAOSYE010000000
VERSION     DAOSYE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_3432_cov_3.350607"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PLU43_03170"
     CDS             <1..1392
                     /locus_tag="PLU43_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO27441.1"
                     /translation="QNKTEDNANASGEGDALAGESAGYSSDSAAAASGPSDSGQADTS
                     SGLSIENTDGEYEAPDISSLVLPDQLKGLTGYEPVQDTGEEIKKKDAEKLKDTIGYGN
                     TGDGLAFDAEMYPYYQMLDESLKSLYRQIYANTNDMTETFVPVETVSASRLKTAFMAL
                     VNDHPELFWLDTAYVYKYTSTGAAEMDLQFNNTADDIDSSKELFNNGANEILSGAKDL
                     SNAYDKEVYVHDRLLDSVSYQLSAPMNQSAYSALVGGKTVCAGYARAFQYLMQQLSVP
                     CYYCTGYAGEDHAWDIIKLDDGYYNVDTTWDDTDPNTYDYFNCSDADYAEDHVRKELS
                     VYLPPCNGGKYRGLLGADAAVSEEEQKRTLQDAGFSEEQVLNDIKEYYDDCYTQLTNQ
                     NETQFTFQNVVKDEALLQEIYAAYKNEGYKSAFMDRFMQEKGIDSCSLNVTVEELDGG
                     YFLISHQVGLPSD"
     gene            1435..2667
                     /locus_tag="PLU43_03175"
     CDS             1435..2667
                     /locus_tag="PLU43_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO27442.1"
                     /translation="MKQLKSYALNTVRYSALQGEFWMSFCVIFSFASVYLLQKGFTST
                     QVGTVIAAAGIISAALQPVVGDIVDKSRHVSLHLAITFFSVLIITAALLLYFLKLPFL
                     LTALVYGVMVAFLQVLTPLVNAIGMHYINRGVDVNFGIARGIGSLCYALISLAVGSLV
                     EHYNESVIPLIIIVIYVLVIAAAVTFRFPGAEAKTEETGETEENEKSSAAGPFYKIYP
                     KFMVLLTGLILLFVSHNMLNNFIFQIVQYYGAGSTEMGRVAGLAAVLELPTMFLFTYY
                     LKIMSAGNLLKISGVFFTLKSLVMYLATGMGMIYAAQFAQMFGFALFVVSSVYYVNIK
                     IERQDQVKGQAYMTMTNTIGSVAGSLLGGWLVDAKGVPAMLLVSTIAAIAGTFIVIFS
                     AEGGYGRVRTGSSARTAS"
     gene            complement(2668..>3432)
                     /locus_tag="PLU43_03180"
     CDS             complement(2668..>3432)
                     /locus_tag="PLU43_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOO27443.1"
                     /translation="YDTLKSGMPNAIRVFKEMREAGLPMPFYGIRMDSGDLAYLSKKV
                     RKMLDDEGFTDAVISASNDLDEFLIDSLKAQGAMITSWGVGTNLITSKDWPAFGGVYK
                     LAAVMGTDGTFIPKIKLSENTEKITNPGNKKIYRVYEKDSHKVKADLICLEEETFHED
                     ESLLLFDPAEPWKKTKMKPGSYTLRELMIPVFKNGTCVYTSPKVMDIRAYCKDELDTL
                     WEETKRLINPHQIYVDLSKKLYDMKISLLDEMSPAE"
BASE COUNT          888 a          676 c          897 g          971 t
ORIGIN      
        1 cagaacaaaa cagaagacaa tgcgaacgca tccggtgaag gagatgcttt ggcaggagag
       61 agcgccggct attcttcgga ttcagccgct gctgcttccg gtccgtccga cagcgggcag
      121 gcagatacgt caagcggact gagtattgag aatacggacg gagagtatga agctccggat
      181 ataagcagtc tggttttgcc ggatcagtta aagggactga caggctacga gccggtacag
      241 gataccggcg aagaaatcaa aaagaaagat gccgaaaagc ttaaggatac aattggttac
      301 ggaaacaccg gagacggact tgcttttgat gcagaaatgt atccgtatta tcaaatgctg
      361 gatgaatcgc tcaaaagtct gtatcgtcag atctatgcga atacgaatga catgacagaa
      421 actttcgttc cggtcgaaac ggtttcggct tccaggctga aaactgcatt tatggcactt
      481 gtcaatgacc atccggagct tttctggctg gatacggctt atgtatataa atatacgagt
      541 accggtgccg cggaaatgga tctgcagttc aacaatactg cggacgacat tgactcttca
      601 aaggaactgt ttaataatgg agcgaacgaa atcctgtccg gggcgaaaga tctttctaat
      661 gcatatgata aagaagttta tgttcatgac aggctgcttg attcggtctc ctatcagctg
      721 agtgcgccga tgaaccagag cgcttacagt gcgctcgttg gagggaaaac ggtttgtgca
      781 ggttatgcaa gggcatttca gtatttgatg cagcagcttt ctgttccgtg ctattactgt
      841 accggctatg ccggcgaaga tcatgcatgg gatattatca agctggatga tggttattat
      901 aatgtagata caacatggga tgatacggat ccgaatacat atgattattt taactgcagt
      961 gatgcggatt atgcagagga tcatgtcaga aaagagctgt ccgtttatct gccgccctgt
     1021 aatgggggaa aatatcgcgg cctgttaggg gcagatgcag ctgtttcaga ggaagagcaa
     1081 aaacgaacac tgcaggatgc cggattttcg gaagaacagg tgctgaacga tattaaggaa
     1141 tattatgatg actgctatac gcagctgaca aatcagaatg aaacgcagtt tacttttcaa
     1201 aatgtagtaa aggacgaggc actgctgcag gaaatatatg cggcctataa aaacgagggc
     1261 tataaaagtg cgtttatgga tcgattcatg caggaaaagg gaatcgattc ctgcagcctg
     1321 aatgtaacgg ttgaagagct tgacgggggt tattttttga tcagtcacca ggtcggtctg
     1381 ccgtccgact aagggtctgt tatattaatg aggacagggt atgggatacg gaatatgaag
     1441 caattaaaga gctatgcgtt gaatacggta agatattctg cactgcaggg agaattctgg
     1501 atgtctttct gcgtgatatt ttcttttgca agtgtgtact tattgcagaa agggtttaca
     1561 agtacacagg tggggaccgt gatagctgca gccggtatca tatcggcggc gctgcagccg
     1621 gttgttgggg atattgtcga taaaagcagg catgtcagcc tgcatctggc aattactttt
     1681 ttttcagttt tgattattac cgcagcattg ctcctgtatt ttcttaagct gccgtttctt
     1741 ctgacagcac tcgtttatgg tgttatggtt gcatttttac aggttttgac acccctggtc
     1801 aatgcaatcg gcatgcatta tataaaccgt ggtgtagatg tcaacttcgg gatcgcaaga
     1861 gggatcggtt ctttgtgtta tgcattgatc tcgctggctg tcgggagtct ggtcgagcac
     1921 tataatgaat cagtcattcc gctgatcatt attgtgatct atgtgcttgt tattgctgca
     1981 gcggtgacat ttcgttttcc aggcgcagaa gcaaaaacag aggaaacagg ggaaacggaa
     2041 gaaaatgaaa aaagttccgc agcaggtccg ttttataaga tctatcccaa attcatggta
     2101 ttgctgacag gattgatcct gctgtttgta agtcataata tgctgaataa ttttattttt
     2161 cagattgtac agtattatgg ggccgggagc acagagatgg gaagagtggc aggacttgcg
     2221 gcagtgcttg agctgccgac catgtttttg tttacgtatt atctgaaaat catgtcggca
     2281 gggaatctgc ttaaaatatc cggcgtattt tttacgctca aatcacttgt catgtatctt
     2341 gcaacaggaa tgggcatgat ttatgccgcg cagtttgctc agatgttcgg gtttgccctg
     2401 tttgtcgttt cgtccgttta ctatgtaaat ataaaaatag aaagacagga tcaggtaaag
     2461 ggacaggcgt atatgacgat gaccaatacg atcggcagtg ttgccggcag tcttttgggc
     2521 ggctggcttg ttgatgcaaa aggcgtcccg gccatgctgc tcgtttctac aattgcagcg
     2581 attgccggga cgtttattgt gattttcagt gccgagggcg gatatggcag agtccgtacg
     2641 ggttcttctg ccagaacggc gtcctgatta ttctgcagga ctcatttcat cgagcagtga
     2701 gatcttcatg tcatacagtt ttttggagag atccacatag atctgatggg gattgatcag
     2761 acgttttgtc tcttcccaaa gggtatccag ctcgtcttta cagtaagcac ggatatccat
     2821 gaccttgggc gaggtataaa cgcaggttcc gtttttgaat acagggatca tcagttcgcg
     2881 gagcgtatag ctgcccggtt tcatttttgt ctttttccat ggttcggccg gatcaaagag
     2941 cagaagcgat tcatcctcat ggaacgtttc ttcttccaga cagatcagat cggccttgac
     3001 cttgtgcgaa tctttttcat aaacacggta gatctttttg tttccggggt tagttatctt
     3061 ttccgtattt tcggacagtt ttattttcgg aataaaagtg ccgtctgttc ccatgacggc
     3121 cgcaagctta taaacaccgc cgaaggcagg ccagtctttg gaggtgatca gatttgtgcc
     3181 gacgccccat gaggtgatca tggcgccctg tgctttcagg ctgtcgatca gaaattcatc
     3241 cagatcattt gatgcggaaa taacggcatc cgtaaatcct tcgtcatcca gcatttttcg
     3301 tacttttttt gataaataag caagatcgcc gctgtccatg cggattccgt aaaacggcat
     3361 cggaagacct gcttcacgca tttccttaaa gacgcggatg gcattgggca taccggattt
     3421 cagtgtgtca ta
//
LOCUS       DAOSYE010000130         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19825_length_3414_cov_3.790414, whole genome shotgun sequence.
ACCESSION   DAOSYE010000130 DAOSYE010000000
VERSION     DAOSYE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19825_length_3414_cov_3.790414"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLU43_03185"
     CDS             <1..355
                     /locus_tag="PLU43_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO27444.1"
                     /translation="RVYKVRDLVEKPSVEKAPSNIAILGRYIITPDIFSYLETQDAGA
                     GGEIQLTDALRRLARDEAMYVYDFKGHRYDVGTKCGFIQANIEFSLRNPETREEMLQY
                     INALHDNMEDMLDYE"
     gene            complement(437..913)
                     /locus_tag="PLU43_03190"
     CDS             complement(437..913)
                     /locus_tag="PLU43_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27445.1"
                     /translation="MSTLTIVLISITVFLLAVVVALYFFGKRTQKKQEAQQAQIDAAK
                     QSVTMLIIDKKRMKIKDSGLPAAVIAQTPRLLRGSKLSIVKAKVGPQMMTLVCDDKIF
                     DIVPTKKEVKAVVSGIYITDVKGLRGGVLKADKKKKGFLKNALEKAQEKVGAKPIK"
     gene            1097..2569
                     /gene="cls"
                     /locus_tag="PLU43_03195"
     CDS             1097..2569
                     /gene="cls"
                     /locus_tag="PLU43_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOO27446.1"
                     /translation="MVQALHDIQWIWNWLMENLVYINLIFSVVIIFFQRRDPKSVWGW
                     LLLLYFVPIFGFVFYIMFGQNYHKSKMFRIKEIEDEINYTIHRQEESILRREFEERKP
                     FVKRYADVVLYNLESTGAVFTDDNDVTIYTDGNRKFDALIEDLKAAEHYIHIQYYIIR
                     DDELFGRIKEVLAERAAHGVEVRVLYDAMGCRNTRHRLWKGLRADGIQTAEFFPALFG
                     RLQLRLNYRNHRKIVVIDGKVGYVGGFNVGREYLGKDKKIGYWRDTHLRITGSAVIHL
                     QLRFILDWNYAAKEDLLKKKQYFEVPEDAVKGKTGIQIVSSGPDSTYKNTRNNYLKLI
                     NRSRDSIYIQTPYFIPDESIFDALRIAALSGVDVKIMIPCKPDHPFVYWATYSYVGDM
                     IAAGAKCYTYDNGFLHAKGLISDGKALSYGTANMDIRSFELNFEVNATIYDEDTAAKM
                     QKVFMEDLTDCTEITAYLYEQRSLFVRIREQVCRLFSPVL"
     gene            2653..>3414
                     /gene="tig"
                     /locus_tag="PLU43_03200"
     CDS             2653..>3414
                     /gene="tig"
                     /locus_tag="PLU43_03200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO27447.1"
                     /translation="MKKSVLIWGLLGVMVCVTGCADKSKIKAEDYISLGEYEGLEVTR
                     VSTEVTDEEIQQQIDSILSDYATTEEITDRTDVQEGDVVNIDYTGTLDGEVFDGGSAQ
                     GFDLEIGSGRFIDGFEEGLIGADVGDTVTLNLTFPGEYSNNPDLAGKDVVFAVKINSI
                     SKSELPEFNDAFIAQESSGQFSTVVDFKAYMTEQMESSRKEYADSIMYTELLDEAVDN
                     AEVIKDIPEEYVQEKIDEMIRTTKSGAEAYGMDFDT"
BASE COUNT          983 a          622 c          890 g          919 t
ORIGIN      
        1 tcgtgtttac aaagtacggg atctggttga aaaaccgtcg gtagaaaaag ccccgtccaa
       61 tattgcgatc cttgggagat atattatcac accggacatt ttttcctatc tggaaacgca
      121 ggatgccggt gccggcggag agatccagct gacagatgcg ctgcgcagac tggccaggga
      181 tgaagccatg tatgtatatg attttaaagg tcaccgttat gatgttggta caaaatgcgg
      241 atttattcag gcaaatattg aattttccct gcgcaatccg gagacaaggg aagagatgct
      301 gcaatatatc aatgcgctgc atgataatat ggaagatatg ctggattatg aataaaattg
      361 aaaagccggc cgctgcggca cagacatcac agcaaaaaac agagccagcg gagatatccg
      421 aaactggctc tgtttattat ttgattggtt ttgcgccgac tttttcctgt gctttttcca
      481 aagcattttt aagaaagccc tttttctttt tatccgcttt caaaactccg ccgcggaggc
      541 ctttgacatc cgtaatataa ataccgctta cgacagcctt tacttctttt tttgtaggaa
      601 cgatatcaaa aattttgtcg tcacagacaa gtgtcatcat ctgcggaccg acctttgctt
      661 ttacgataga cagcttggag ccgcgtaaaa gtctgggtgt ctgtgcgatc acagccgcgg
      721 gcagaccgga atctttgatc ttcattcgtt ttttatcgat gatcagcatc gtaacggatt
      781 gctttgcggc atctatctgc gcctgctgtg cctcctgctt tttctgcgtt cttttgccga
      841 agaaataaag ggcgaccacg acagcaagca gaaaaaccgt aattgaaatt agtacgatgg
      901 ttaacgttga cacggtatac ctcctcaata aatataatgt tgataaataa gcctttttgt
      961 taattaacag aggcgaattt tccataaacg gaaccaaata atattgtatc acaactagat
     1021 tgctgtgcgc aagagataaa gttgaacagc cgggcacaga agtctgtaat caatatcttt
     1081 gcggggggat tttgaaatgg tgcaggcatt gcatgatata caatggatat ggaactggct
     1141 gatggaaaat ctggtttata tcaacctgat tttttccgtt gttattattt ttttccagag
     1201 acgggatccg aagtccgtct ggggctggct tttgctgctt tattttgttc cgattttcgg
     1261 atttgttttc tatatcatgt ttggacagaa ttatcataaa agtaaaatgt ttcggatcaa
     1321 ggaaatcgag gatgagatca actatacgat ccaccggcag gaagaaagta ttctgcggcg
     1381 tgagtttgaa gaacggaagc cgtttgtaaa acggtatgcg gatgttgttt tatataatct
     1441 ggaaagtacg ggtgcggtat ttacggatga caacgacgtt acgatttata cggacggaaa
     1501 cagaaagttt gatgccctga tcgaagatct gaaggcagcg gagcattata ttcatataca
     1561 atattatatt attcgagacg atgaactgtt tggccggata aaagaagtgc ttgcggagag
     1621 agccgcacac ggtgtggagg tcagggtcct ctatgatgcg atgggctgca gaaatacaag
     1681 gcacagactg tggaagggtc ttagggcgga cgggatccag accgcagagt tttttccggc
     1741 gctgttcggc cgtttgcagc tgcggctgaa ttaccgcaat catagaaaga tcgtggtgat
     1801 cgatggaaag gtcggttatg tcggaggctt taatgttgga cgggaatatc tcggaaaaga
     1861 taaaaaaatc ggatattgga gggacacaca tttaaggatc acgggttcgg ctgttattca
     1921 tctgcagctt cggttcatac tggactggaa ttatgccgca aaggaagatc tgcttaagaa
     1981 gaagcagtat tttgaagtac ctgaggatgc ggtaaaaggg aagaccggga tccagatcgt
     2041 gtcgagtggt ccggattcta cttataaaaa tacaagaaat aattatttga aactgatcaa
     2101 tcgttccagg gacagtattt atatccagac gccatatttt attccggatg aatctatttt
     2161 tgatgcactg cgcatagcag cattgtccgg agttgacgtt aagatcatga taccgtgcaa
     2221 accggatcat ccgtttgttt actgggcaac ctattcctat gtcggggata tgatcgcggc
     2281 cggagcaaaa tgctatacgt acgataacgg atttctgcat gcaaaagggc tgatttcaga
     2341 cggcaaggca ttaagttatg gaacggcaaa tatggatatc cggagttttg aactgaattt
     2401 tgaagtcaac gcaacgatct atgatgaaga taccgcagca aagatgcaga aagtgtttat
     2461 ggaggatctg accgattgta cggagatcac agcatatctg tatgaacagc gaagcctttt
     2521 tgttcgtatc agagaacagg tgtgccgtct gttttccccg gtcttgtaga aagccagaaa
     2581 gccctttatt ttggagcatg gttgtgataa actaatcgga aaggtgttta taagagcaga
     2641 aaggaattat ctatgaaaaa aagtgtactc atatggggat tattgggtgt aatggtctgc
     2701 gttacaggct gtgcagacaa aagcaagatc aaggcggaag attatatttc tcttggagaa
     2761 tatgaaggac tggaagtcac aagggtgtct actgaggtga ctgatgaaga aatacagcag
     2821 cagatcgaca gtattttaag tgattatgcg acaacggaag agatcacaga ccgaaccgat
     2881 gtacaggagg gggacgtggt caatatcgat tatacgggaa ctttggacgg ggaagttttt
     2941 gacggcggat ccgcacaggg ctttgatctg gagatcggtt ccggccgttt tatcgacggg
     3001 tttgaagaag gactgatcgg tgcggatgtc ggggatacgg tcacgctgaa tctgacgttt
     3061 ccaggcgagt attcaaacaa tccggatctg gcaggcaagg atgtcgtatt cgcggtcaag
     3121 atcaattcca tcagtaaaag tgagctgccg gagttcaatg atgcgtttat cgcacaggag
     3181 agcagcgggc agttttcaac agtcgtggat tttaaggcgt atatgacgga gcagatggag
     3241 agcagcagaa aagaatatgc cgacagcatc atgtatacgg agcttttaga tgaagcggtc
     3301 gataacgcag aggtcatcaa ggatattccg gaagaatatg tgcaggaaaa gatcgatgaa
     3361 atgatcagaa cgaccaaatc aggcgcagaa gcatacggca tggattttga taca
//
LOCUS       DAOSYE010000131         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19928_length_3403_cov_3.350060, whole genome shotgun sequence.
ACCESSION   DAOSYE010000131 DAOSYE010000000
VERSION     DAOSYE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19928_length_3403_cov_3.350060"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLU43_03205"
     CDS             <1..460
                     /locus_tag="PLU43_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27448.1"
                     /translation="ELQACGFNREASKYAGINEKRGIVLSMTIAGAISGMAGGLLYLA
                     GTGKHIEIVDTIADEGFTGISVAMLGLSHPVGVLLAGIFIAYLTAGGFYLQLYEFSTE
                     IIDIMIAVIIYFSAFSLIIKTFIIKAHMKRTEDKTKAPPASKHTKEGGDN"
     gene            463..1416
                     /locus_tag="PLU43_03210"
     CDS             463..1416
                     /locus_tag="PLU43_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27449.1"
                     /translation="MSVIYFIFQQTMFFTIPLLIVALGGMFSERSGVVNIALEGIMTM
                     GAFCSILFLNLTDSHMSGQGQLLLAILIAMASGMLFSLFHAYASINMKADQVISGTAL
                     NMFAPAFAIFIARVIQGVQQIQFTNTFRIDSVPLLGRIPVLGPLLFQNTYITTYLGIA
                     ILILSWIVLYKMRFGLRLRACGEHPQAADSAGINVYKMQYAGVMISGALGGLGGLVFV
                     VPTSTNFNATVSGYGFLALAVLIFGQWKPWKIMWASLFFGLMKAIASAYSGIPFLAAM
                     NVPSYLYKMIPYIATLIVLIFTSKNSQAPRASGVPYDKGAR"
     gene            complement(1505..2971)
                     /locus_tag="PLU43_03215"
     CDS             complement(1505..2971)
                     /locus_tag="PLU43_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO27450.1"
                     /translation="MDNKNIWSPVKFDFEWNNAETRKFDNILPSWEKRRDELNKYPEQ
                     YKRFIDQLKRKQAIDTGIIERMYDLKKGVTETFIKEGFAESYLQHGDTDIAPDLLMDY
                     LRDNFDAIDLIFDFVKNDRPLSIGYIKELHSLITQHQDSTDAIDQFGRHVKVELLKGE
                     FKKYPNNPVRDDVIYNYCPPEQVDVEMDNLVRIFNEELKTSHILVRAAFFHHAFVQIH
                     PFQDGNGRIARLLASFVLIKKGLFPLSIDRGDRKKYIDALESADNHVYQPIIDVFAHN
                     QIASIEQALNWKTIEGMEEYDSVLNILSQKLSDYRSETAEQKNRCILNNMQKIFDVLK
                     LQLEHFKDDLVQKLDVNANIDVVFCDPDDSDTYYYTKQIIEYAKKFNYYANLSLSKCW
                     GRMFIKIDEMKKYRLVLSVHHYGYDNSTFAIGAFLSEVISEEYVHGKIQREYVDIPLG
                     VPPLIMSSEKEVSELESSIKQQTELFIMAALAYIVNEL"
     gene            complement(3147..>3403)
                     /locus_tag="PLU43_03220"
     CDS             complement(3147..>3403)
                     /locus_tag="PLU43_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO27451.1"
                     /translation="EIADKAKGKKSAIKWLREKMKTLPQEEGLPMLVGHADAVSRGEA
                     VKEVLEADGISGILDNVCMGSVVGTYSGPDAIGIFYVSKK"
BASE COUNT          921 a          782 c          628 g         1072 t
ORIGIN      
        1 tgaactgcag gcctgcggct tcaacagaga agcaagcaag tacgccggta tcaatgaaaa
       61 gcgcggcatc gtactttcca tgacgattgc cggtgcgatc tccggtatgg ccggcggatt
      121 actgtatctc gccggaacag gaaagcatat cgagatcgtc gatacgatcg ccgacgaagg
      181 ctttaccggt atttccgttg caatgcttgg tctctcccat ccggtcggcg ttctgctggc
      241 aggcattttt atcgcatacc tgacagccgg cggattttac ctgcagcttt acgaattttc
      301 aactgagatt attgatatca tgattgccgt gatcatttat ttcagtgcct tttccctgat
      361 catcaaaaca tttattataa aagcgcatat gaaaaggacg gaagacaaaa caaaggctcc
      421 gcctgcatcc aaacatacaa aggaaggagg ggacaactga ttatgagtgt tatttatttt
      481 atctttcaac agacgatgtt ttttaccatt ccacttttga tcgtagctct cggcggtatg
      541 ttttcagaac gaagcggcgt tgtcaatatc gcgctggaag gaatcatgac aatgggcgca
      601 ttctgcagca ttctgtttct caatctgaca gattcccata tgagcggaca gggacagctc
      661 ctgcttgcca tcctgatcgc aatggcctcc ggcatgctgt tttcgctgtt tcatgcctat
      721 gcttccatta atatgaaagc tgatcaggtt atttccggta ccgcgctcaa tatgtttgcc
      781 cctgcttttg caatttttat tgcacgtgtg attcagggcg ttcagcagat ccagtttacg
      841 aacacgttcc ggatcgactc cgttccgctg ctcggcagga ttccggttct cggaccactg
      901 ctgttccaga atacgtatat tacgacttat ctcggcattg ccatactgat cctctcctgg
      961 atcgtattat acaagatgcg ctttggcctg cgtctgcgcg catgcgggga acatccgcag
     1021 gcagccgaca gcgccggcat caatgtttac aaaatgcagt atgcaggtgt tatgatttcc
     1081 ggagccctcg gcgggttggg cggtcttgtt ttcgtcgttc cgacttcaac gaactttaat
     1141 gccacggtaa gcggctacgg cttccttgct ctggccgtac tgatctttgg gcagtggaaa
     1201 ccttggaaga tcatgtgggc ttccctgttc tttggactga tgaaagccat tgcttcggct
     1261 tattccggaa ttccgttcct tgccgccatg aatgtaccaa gttatctgta caaaatgatt
     1321 ccttatattg cgaccctgat cgtcctgata tttacgtcca aaaacagcca ggctccaaga
     1381 gcttcgggtg ttccttacga caaaggcgcc agataatcga ataaatgata aatgagagtg
     1441 ttcagcaaac tgttttacag cttgttaaac actctcattt tatttttttt cctattataa
     1501 tctgtcaaag ttcatttaca atatatgcaa gcgcagccat aatgaacaat tctgtctgct
     1561 gcttaatgga tgattccaat tcagaaactt ctttttcaga tgacattata agcggcggaa
     1621 ctccaagtgg aatatctaca tactctctct ggattttccc atgtacatat tcttctgaaa
     1681 ttacctctga aagaaatgcg ccgattgcaa acgtgctatt gtcgtaacca taatgatgga
     1741 ctgacaacac tagccgatac tttttcatct catcaatttt tataaacatt cgtccccagc
     1801 atttactcaa tgatagattt gcgtaataat taaacttttt cgcatattca ataatttgtt
     1861 ttgtataata atatgtatca gagtcatctg gatcacaaaa aacaacatca atatttgcat
     1921 tcacatcaag tttctgaacc aaatcatctt taaagtgctc caactgaagc ttcaatacgt
     1981 caaaaatctt ttgcatgtta tttaaaatac atcgattttt ctgttcagcc gtttcagaac
     2041 ggtaatcaga cagcttctgg ctaagtatat ttaatacact atcgtattct tccatgccct
     2101 caatagtctt ccaattaagt gcctgttcta ttgaggctat ctgattatgg gcaaacacgt
     2161 caatgattgg ctgataaacg tgattatcgg cactttccag tgcatcaata tattttttcc
     2221 tatcaccgcg gtcaatcgaa agagggaaaa gtcctttttt aataagaaca aaacttgcca
     2281 ataaacgagc tatacgtcca ttcccatcct gaaatggatg tatctgaaca aacgcatgat
     2341 gaaaaaaagc tgccctgact aaaatatgcg acgttttaag ttcttcatta aagatacgaa
     2401 ctaaattatc catttccaca tcaacctgct ccggtggaca ataattataa atcacatcat
     2461 cccgtaccgg gttattaggg tactttttaa attccccctt aagtaactcc actttaacat
     2521 gcctgccaaa ctgatcaatt gcatcggttg agtcctggtg ctgtgtaatt aaactatgca
     2581 gttctttgat ataaccaata cttaacggcc gatcattttt tacaaaatca aaaatcaaat
     2641 ctattgcatc aaaattatcc cgcaggtaat ccataagtaa atctggtgca atatctgtat
     2701 caccatgctg aagatatgat tctgcaaacc cctccttaat aaaggtttcc gttactcctt
     2761 ttttcaggtc atacattcgt tcgataatac cggtatctat cgcctgtttt ctctttaact
     2821 ggtctatgaa gcgcttatat tgttcgggat acttgtttaa ttcatcacgt cttttttccc
     2881 aggaaggaag aatattatca aatttccttg tctccgcatt attccattca aaatcaaact
     2941 taacgggact ccatatattt ttattgtcca tcttcctgcc cactcctttc acatcttata
     3001 tcacagttat agtaactatg ataaggtatc tcttcaattt taacaaccat aattttagag
     3061 gcattttcaa atttcaaaat ataaaaaatg acagtgaaaa agtgaattca ttgtcatttt
     3121 cattgtaaat caaaatttat actttattat tttttcgaaa catagaaaat accgatcgcg
     3181 tccggtccgg aataggtacc gactacggaa cccatacaga cattatccag aataccgctg
     3241 attccgtccg cttccagtac ttccttcacc gcttctccgc ggctgaccgc atccgcatgg
     3301 ccgacgagca ttggaaggcc ttcctcctgc gggagtgttt tcattttttc gcgaagccat
     3361 ttaatggctg atttttttcc ttttgcctta tctgcaatct cta
//
LOCUS       DAOSYE010000132         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_19929_length_3403_cov_3.172939, whole genome shotgun sequence.
ACCESSION   DAOSYE010000132 DAOSYE010000000
VERSION     DAOSYE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19929_length_3403_cov_3.172939"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLU43_03225"
     CDS             <1..496
                     /locus_tag="PLU43_03225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO27452.1"
                     /translation="HVCEAVEKLNAKPGNPSLTLTVIGDTGLDTEYINSFPFVENKGL
                     LPFEEAKALLYSSELFVQNSCFETFGLAPLEALACGCSILMSKNVGALELFRRTDEAD
                     VIENYEDADEIASKISSLLQHGNAKKLLAELDKESISWEARTNQLSAKLAQLESDVQK
                     YGKK"
     gene            418..1635
                     /locus_tag="PLU43_03230"
     CDS             418..1635
                     /locus_tag="PLU43_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27453.1"
                     /translation="MGSKDEPVISEAGTVRIGRSKIRKEIINWSVFLLLFAGYTLTFM
                     VPKFYGATDSYVGVFVFLMCSVLFFNNAGIRELWKTKDKETMILAGIILITGINLLIV
                     DSGKGAFFAASNFALIWYLSDKIVLTRRQVTVFAGLYTLLLFYWLFIAYPKMFSNYET
                     YAYNTNTAATFTVFTMLCSFVLLQRIYETHEIAGLFMVIILLKGIQLVLWHRARGAFI
                     MLVVFIFFNFIVPKKWWGNKKFFGFLCLFATLGSLAFVCLYVLIGQTGVNFQIPFFYK
                     EVFSGRNKIWYEFWTLFVKKPITGIGTNVELESFFEFNVHNAMYNLLVVHGIVVFAGI
                     LYFVYKRLYRFRRIIVNNRLALCALSAVFAVFFESYFDVDLIWADYALNLMFLLAVVN
                     HKDGAKRTEGTNG"
     gene            1628..3067
                     /locus_tag="PLU43_03235"
     CDS             1628..3067
                     /locus_tag="PLU43_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOO27454.1"
                     /translation="MGKYKYLANNIALFSISNLVSKILVFLLVPFYTSVLTTAEYGVA
                     DIFQVTLLLLVPALTVNMGEAALRYGIEKENKRKGILRVGLKYTFLGAALVALAAYAS
                     AFFVSDRIRWYLFLFVLLFLSNALYEFLILYFQGCELVKIVVAGSVFSTVCMISCNLY
                     FLLVLKIGLNGYLFSQMIAYFASVCLMLLLSRKYIGKGLPKDEELEKEMLSYGKPLLL
                     YSTGSWINNAADRYIVAGICGTAVNGIYGVAYKIPAILMVFQRIFAQAWQMSATKSYQ
                     DKNSSEFFSMMYRTYHMFMVLGCSVLILFVRAIAWILFKKDFYEAWQFVPPLLISVIF
                     GALTGFLGSICLAHKDSRSMGYATGIGALINLLLNFLTVPKYGALGAAASTAVSYFSM
                     YFMAFKIVKKYVKIETRPVRNYVSYILLIVQAVTMINSIGPFYVINGLIFLLLFLLYA
                     KEAADVFGKMKGSLRKGMTGHGKTNDLQS"
     gene            3039..>3403
                     /locus_tag="PLU43_03240"
     CDS             3039..>3403
                     /locus_tag="PLU43_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27455.1"
                     /translation="MEKQMIYNHKGISEKRKRAGNIALLTGVFLLITAGWNVHAWYEA
                     TAPYGTLVAAAALITAFFCYVNIKDALHDPAFYLMAAADLIALCHLFMIHSHKGAILV
                     VADFLLILYLANKVVFSNR"
BASE COUNT          851 a          632 c          856 g         1064 t
ORIGIN      
        1 gcatgtctgc gaggcggtag agaagctgaa cgcaaaacca ggaaatcctt cccttacatt
       61 aaccgtgatc ggagataccg gactggatac ggaatatatc aacagctttc cgtttgttga
      121 aaataaagga ctgctgccgt ttgaagaagc taaggcgctt ctttactctt cagagctgtt
      181 tgtgcagaac agctgttttg aaacgtttgg tcttgcaccg ctggaagcac ttgcatgcgg
      241 ctgcagtatt ctgatgtcaa aaaacgtagg ggcgctggaa ttgttccggc gaacggatga
      301 agcggatgtg atcgaaaatt acgaggatgc ggatgagatc gcatcaaaaa tcagcagtct
      361 gctgcagcat ggaaacgcca aaaagctgct tgcagaactt gataaggagt ctatttcatg
      421 ggaagcaagg acgaaccagt tatcagcgaa gctggcacag ttagaatcgg acgttcaaaa
      481 atacggaaag aaataatcaa ttggtccgta ttccttttat tgtttgcagg ttatacactt
      541 acatttatgg ttccgaagtt ttatggggca acggattctt atgtaggtgt atttgtgttt
      601 ttgatgtgca gtgtgctttt ctttaataat gccggtatca gggaactgtg gaagacaaaa
      661 gataaggaaa caatgattct tgccggtatt attttaatca cagggatcaa tttgctgatc
      721 gttgattccg gaaaaggtgc tttttttgcg gcatcaaatt ttgcactgat ctggtatctg
      781 tcggataaga tcgtgctgac ccggcgccag gtcacggtat ttgcaggtct gtatacactg
      841 ctgctgttct attggctgtt catcgcctat cctaaaatgt tcagcaatta tgaaacctat
      901 gcctataata cgaatacggc ggcgacgttc accgtattta caatgctatg ctcctttgtg
      961 cttttacagc gaatatatga aacacatgag atcgccgggc tgtttatggt gatcatcctg
     1021 cttaagggca tacagctcgt actgtggcac agagcaagag gagcgtttat tatgctcgtt
     1081 gtgttcattt ttttcaattt tattgttccg aaaaaatggt ggggcaataa aaagtttttt
     1141 ggatttctat gtctgttcgc aacacttggg tcacttgctt ttgtgtgcct ctatgttttg
     1201 atcggacaga ccggtgttaa ttttcagatc ccgttttttt ataaagaggt gttttccgga
     1261 agaaataaga tctggtatga gttttggaca ttatttgtga aaaagcccat tacggggatc
     1321 ggtacgaatg ttgagctgga atcgtttttt gaattcaatg tgcacaatgc catgtacaat
     1381 ctgctggttg ttcacggaat cgttgtgttt gcgggcattc tttattttgt ttataaacgg
     1441 ctgtatcgat ttcgcaggat catagtaaat aacaggctgg cactttgcgc gctcagtgcc
     1501 gtttttgcgg tattttttga gtcctatttc gacgtggatc tgatctgggc ggattatgcg
     1561 ctgaacttga tgtttctatt ggcagtagtc aatcataaag acggagcgaa aaggacagaa
     1621 ggaacaaatg ggtaaatata aatatctggc taataatatt gcactgtttt ccatcagtaa
     1681 tttagtttcc aaaatactgg tttttttgct tgtaccattt tatacaagtg tattgacaac
     1741 tgcggagtat ggggttgcgg atatttttca ggtgaccctg cttctgcttg tacctgcgct
     1801 cacagtaaac atgggagaag ccgcattaag atatggaata gaaaaagaaa ataaaagaaa
     1861 agggatcctt cgggtcggtc tgaaatatac gtttcttggc gcagcacttg tggcgcttgc
     1921 ggcgtatgct tcagcctttt ttgtgtctga ccggatcagg tggtatctgt tcctgtttgt
     1981 actgctgttt ctgtcaaatg cgctttatga gtttctcatt ttgtattttc agggctgtga
     2041 gcttgtaaag atcgttgtgg cagggagtgt gttttcgaca gtatgtatga tttcctgcaa
     2101 tctttatttt ttgcttgtac tgaagatcgg tctgaatggg tatctgtttt cgcagatgat
     2161 cgcttatttt gcgtcggtct gcctgatgct gctgctttcc agaaagtata tcgggaaggg
     2221 gctgccaaag gatgaggaac tggaaaagga aatgctctcc tacgggaagc cgcttctgct
     2281 ttattcgacg ggcagctgga tcaacaatgc ggccgaccgc tatatcgtag cgggtatctg
     2341 cggtacggca gtcaacggta tttacggagt tgcctataaa atacctgcga tccttatggt
     2401 attccagcgg atctttgctc aggcatggca gatgtccgcg acaaagagct atcaagataa
     2461 aaacagcagt gaattttttt ccatgatgta tcgtacctat catatgttta tggtgcttgg
     2521 ctgctccgtg ctgattctgt ttgtaagggc aattgcctgg atcttgttta aaaaagactt
     2581 ttatgaagca tggcagtttg taccgccgct gctgatttcg gtcatattcg gggcactgac
     2641 cggtttcctc ggttcgattt gtctggcaca caaagacagc agatcaatgg gttatgcgac
     2701 aggaatcgga gcgctgatca acctgctgct gaattttctg actgttccga aatacggcgc
     2761 gctgggagct gcggcgtcta ctgctgtttc ttatttttcc atgtatttta tggcgtttaa
     2821 aatcgtgaag aaatacgtaa aaatagaaac aaggccggtt cggaattatg tttcctatat
     2881 tctgctcatt gttcaggcgg tgacaatgat aaattccatc gggccgtttt atgtgataaa
     2941 cggattgatt tttctgctgc tgtttctgtt gtatgcaaaa gaagcggctg acgtgtttgg
     3001 caaaatgaaa ggatcattgc ggaagggaat gacaggtcat ggaaaaacaa atgatttaca
     3061 atcataaggg aatcagtgaa aaaagaaaac gggcagggaa tatcgcactg ctgaccggtg
     3121 tattcctttt gattaccgcc ggctggaatg tacatgcgtg gtatgaggca acggcgccgt
     3181 atggaacact tgttgctgcg gctgcgctta tcacggcatt tttctgttat gtcaatataa
     3241 aggacgcgct tcatgacccg gcattttacc tgatggctgc ggcagatctg atcgcattgt
     3301 gtcatctttt tatgatccat tcccacaaag gggcgatcct tgttgtggcg gattttctgc
     3361 tgatcctgta tctggccaat aaggtagtgt tttcaaacag gca
//
LOCUS       DAOSYE010000133         3387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20100_length_3387_cov_2.932173, whole genome shotgun sequence.
ACCESSION   DAOSYE010000133 DAOSYE010000000
VERSION     DAOSYE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20100_length_3387_cov_2.932173"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLU43_03245"
     CDS             <1..955
                     /locus_tag="PLU43_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27456.1"
                     /translation="ADYTESYFTPFKDGDKIYALPVLTGGTCTVVVYDKAMWQEAGFD
                     TFPSTWEDVKTAMDYFSGEGIDTIAFGNGGQWQANSCFLSCLGDRFTGPDWFTSLINK
                     DGAAFTDQSQVDALAYTQELFQSGIFNEDFNTITNEDAREYYIAGDAAAFIGGNWDES
                     YIYATLKETDEDKLNNMGFAVLPQPAEAEYDPQGQNIGLGYGMAINSKLADDPDKLAA
                     AIDLCYKLTGPDFANYVATNYALGGLTKVADVDLSAFDQITQDFYNWSYVDTTPCEIY
                     DSYISSAVWSVYNTDVQEMLNGDMTAEEVAADAQAAYEENY"
     gene            1216..2112
                     /locus_tag="PLU43_03250"
     CDS             1216..2112
                     /locus_tag="PLU43_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27457.1"
                     /translation="MLKSIKPKASTVIAYLAIPFVWYVFSVFVPLITAMFYSLFEWKG
                     GPQKSFIGLENYSKLISDGTFWMSFGHNLFLVVACIIGQIGLAFIFVLMINSRLVKLK
                     GIHRTFGFFPSTISAVALGFIWKMIYDNKRGVLNWFLTAIGRDDLTKVWLNDKSIIML
                     LVAIPLVWQFIGYYMVILLSGIAAIDTEIFEVAEIDGANAVQRAIYIVMPLLKNTLLV
                     CVTLCVAGNMKAFDHIYTMTEGGPGTASMVMAMYGYKISFEQMNMGYGSAISVGIFVV
                     SLLVIAGSQALINFYTKDKGVD"
     gene            2114..3001
                     /locus_tag="PLU43_03255"
     CDS             2114..3001
                     /locus_tag="PLU43_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27458.1"
                     /translation="MAKNAVGIAGKKKSTAGEKAKKGILAFILNAILIIFSATCIFPL
                     IWMFYSSLKPKRVFNADIIGLPKEPTLVNYQKITSNADYHIWQSMGNSARTTILSLFF
                     IVLFGFIVGYILARVQFKGNRALYVMFLMGMLIPVHSLLVPIYVVFNNTGLGNQWFTL
                     IIPYVAFGLPIAVFLVESFIKSIPTEMEQAAAIDGSSFSRTLFSIILPITRPMLVTIT
                     IIQTFSCWNEFSFALVLMQDVRLQTVPLAMTMFKGQFASDYPKIMSAMLLTMSPIVVL
                     YFVFSKEIIKGMVAGAVKG"
     gene            complement(3218..3385)
                     /locus_tag="PLU43_03260"
     CDS             complement(3218..3385)
                     /locus_tag="PLU43_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO27459.1"
                     /translation="MDYVRISHAKMLLTQSHMKIYEISERVGYKNVDYFYKKFRMVTG
                     TTPADYRQNLP"
BASE COUNT          936 a          621 c          794 g         1036 t
ORIGIN      
        1 agcagactat acggaatctt atttcacacc gtttaaggac ggagacaaga tctatgcact
       61 tcctgtactg acaggtggta catgtacagt tgttgtttat gataaggcta tgtggcagga
      121 agctggattt gatacattcc cgtcaacatg ggaagatgtg aagacagcta tggattactt
      181 ctcaggtgaa ggcattgata cgatcgcatt tggtaatggc ggacagtggc aggcaaattc
      241 ctgtttcctt tcctgcttag gcgacagatt tacaggtcct gactggttta catccctgat
      301 caacaaggat ggcgctgcat ttacggatca gtcacaggtg gatgctttgg cttatacaca
      361 ggaactgttc cagtccggta tctttaatga agacttcaac acgattacaa acgaagatgc
      421 acgtgagtac tatattgcag gtgatgcagc agcattcatc ggcggtaact gggatgagtc
      481 ttatatctat gctacattga aagaaacaga tgaagacaag cttaacaaca tgggatttgc
      541 agttcttccg cagcctgcag aagctgaata tgatccgcag ggacagaata tcggcttagg
      601 ctatggtatg gcaatcaatt ccaagctggc tgacgatccg gacaagcttg ctgcagcaat
      661 cgatctttgc tacaagctga caggtcctga ttttgcaaat tatgtagcta ccaattatgc
      721 tttaggtgga cttacaaagg ttgctgatgt tgatctgagc gcattcgatc agatcacaca
      781 ggatttctac aactggtcat atgttgacac gactccttgt gagatctatg attcttatat
      841 ctccagtgca gtttggtccg tatataacac agatgttcag gaaatgctga acggcgacat
      901 gacggctgaa gaagttgcag cagatgcaca ggcagcttat gaagagaact actaaaatat
      961 aatagcagct tcaaaaaaat atctgagtcc acaacagcgt ggattgaaat tgcgggtctc
     1021 gtccgactca aggatgagac ccgtatttat gcagaataca aattttattt aagtttaata
     1081 ttattgagac tgccatgcag atatgtgata gaataagtga tagatacgtg attcatgaac
     1141 agagtcaaaa tgacgatgcg aatcattttg agtctgttta tgggaatgga gtaaaaatga
     1201 aggaggtttt atgaaatgct gaaatctatt aagccaaaag caagtacagt catagcatat
     1261 ttggcgatcc cttttgtctg gtatgtattt tctgtgttcg taccattaat tacagccatg
     1321 ttttacagcc tttttgaatg gaagggaggc ccgcagaaat cttttatagg attggaaaat
     1381 tattctaaac tgatttcgga cggtacgttc tggatgtctt tcggacataa tctgtttctt
     1441 gtagttgcct gtatcattgg tcagatcgga cttgcattta ttttcgtttt gatgatcaat
     1501 tccagacttg tgaaattaaa agggatccat cgtacattcg gatttttccc aagtacgatt
     1561 tctgccgtgg ccttaggttt tatctggaaa atgatctatg ataataaaag aggcgtgctg
     1621 aactggtttt taacagcgat cggcagagac gacctgacaa aggtttggct gaatgataag
     1681 agcattatca tgctgcttgt tgcgatccct cttgtatggc agtttatcgg ttattatatg
     1741 gtcattcttt tatccggtat tgcggcgatc gatacggaaa tatttgaagt ggcggagatc
     1801 gatggtgcca atgctgttca aagagccatt tatatcgtaa tgccgctttt gaaaaatacg
     1861 ctgcttgttt gtgtgacgct ttgtgttgca ggtaatatga aggccttcga tcatatttat
     1921 acgatgacag agggcggacc cggcactgca tccatggtta tggcgatgta tggttacaag
     1981 atctcattcg agcagatgaa tatgggatac ggcagtgcga tatcagtcgg tatttttgtt
     2041 gtcagtcttc ttgtaattgc aggcagtcag gcacttatta atttctatac aaaagataag
     2101 ggggtagatt gatatggcaa aaaatgcagt tggtattgcc ggtaagaaaa aatctactgc
     2161 cggtgaaaaa gcgaaaaaag gtattcttgc atttattctg aatgcaattt taattatatt
     2221 ttccgcaacc tgtattttcc ctttgatttg gatgttttat tcttctttga agccaaagag
     2281 agtatttaat gctgatatta tcggcctgcc gaaggaacct acgctggtca attatcaaaa
     2341 gatcacgtca aatgccgatt atcatatctg gcagtccatg ggaaacagtg caaggacgac
     2401 gatcctttct ttgttcttta tcgttctgtt tggctttatt gtcgggtata ttcttgccag
     2461 agttcagttt aagggaaaca gggctctcta tgtaatgttt ctgatgggca tgctgattcc
     2521 tgtgcattca cttctcgtac ctatttatgt tgtgtttaat aatacgggac ttggcaatca
     2581 gtggttcacg ctgataattc cctatgtggc atttggcctt ccgatcgcgg tatttcttgt
     2641 agaaagcttt attaaatcga taccaacgga aatggagcag gcagcggcga tcgacggcag
     2701 cagcttttcg agaacattgt tttccattat tcttcctatt acaaggccaa tgcttgtcac
     2761 gattacgatt atccagacat tttcctgctg gaatgagttt tcctttgcat tggtattgat
     2821 gcaggatgtc aggctgcaga cagtaccgtt ggccatgaca atgttcaagg gtcagttcgc
     2881 atctgattat ccgaagatca tgtcggccat gctgcttacg atgtcaccga tcgtggtcct
     2941 ctacttcgtc ttcagcaaag agattatcaa agggatggta gcaggagccg tcaaaggtta
     3001 acttaataag aagccaatgc gccgagcgca agcgagtgtg caaaaaaagg tatggtgctt
     3061 tgcaccatgc catggtagca ggagctgtta aaggctaatt agataagaag ccaatgcgcc
     3121 gagcgtaagc gagtgtgcaa ggtaaagtat catataaata tagaacaaaa taactgccgg
     3181 ccggcagtta ttttgttcct gttatgagaa aaaattttta cggcagattc tgccggtagt
     3241 cagctggtgt cgtgccggtg accatacgga attttttata aaagtaatcg acatttttat
     3301 atccgacccg ttctgatatt tcataaattt tcatatggct ttgtgtcagc agcattttgg
     3361 catgagatat ccgtacataa tccaaat
//
LOCUS       DAOSYE010000134         3385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20117_length_3385_cov_3.512012, whole genome shotgun sequence.
ACCESSION   DAOSYE010000134 DAOSYE010000000
VERSION     DAOSYE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20117_length_3385_cov_3.512012"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /gene="ygfK"
                     /locus_tag="PLU43_03265"
     CDS             complement(<1..1850)
                     /gene="ygfK"
                     /locus_tag="PLU43_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOO27460.1"
                     /translation="MGDKMTPLSFEQLLNWIFGEKEAGGAVFGLHGSYFAEKERRLSL
                     FGEMLETPFGPAAGPHTQLAQNLAAAYYAGGRFFELKTVQKLDGDDLPVSKPCIMADD
                     EGYNVEWSTELYVPQAFEEYVKAWFLMHLMAKEFGLGASDGFIFNMSVGYDLDGIKTK
                     KIDDFIEGLKDASDTPVFKECMEYALRHTELFSNVSKEDIEQIPAEICSSITLSTLHG
                     CPPQEIERIATYLLTQKQLHTFIKCNPTLLGYEFARKTLDKMGYDYVSFTDFHFKDDL
                     QYEDAVPMLTRLMSLASEKGLEFGVKITNTFPVDIKAGELPGEEMYMSGRALYPLSIA
                     LAAKLSEEFDGRLRISYSGGADIHNIEQIVDAGIWPVTMATTALKPGGYARFIQIAKA
                     FHGKQFSSFAGVDVKKAKVLAEAAVFDKHHRKPVKPHQNRKLKKELPLFDCFVAPCQE
                     ECPIHQDVMTYMRLVEQGEYQKALEVIMEKNPLPFTTGTICSHRCMSSCTRNFYDDPV
                     RIRQMKLIAAENGYDRILENLTAKTMDKAAGKIAVVGGGPAGISASYFFAGAGFAVTM
                     FCNKETPGGVARFVIPEFRITGDAIEKDISFIKKLGVEIREMTKITSLDEL"
     gene            complement(1890..3222)
                     /gene="ssnA"
                     /locus_tag="PLU43_03270"
                     /pseudo
     CDS             complement(1890..3222)
                     /gene="ssnA"
                     /locus_tag="PLU43_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
BASE COUNT          852 a          932 c          641 g          960 t
ORIGIN      
        1 agttcatcaa gagacgtgat ctttgtcatt tccctgatct caacacccag cttcttaata
       61 aaggaaatat ccttttctat tgcatcgcct gtaatacgga actccggaat cacaaatctg
      121 gcgacaccgc ccggtgtttc tttattgcag aacatggtga ctgcaaagcc ggcacctgca
      181 aagaaatacg aagctgaaat accggccggg cctccgccga caacagcgat cttaccagcc
      241 gctttatcca tagtttttgc cgtcaggttt tccaatatgc gatcgtatcc gttttcggca
      301 gctatcagct tcatctgcct gatccgcacc ggatcgtcgt aaaaatttct cgtacaggaa
      361 ctcatacagc gatgggaaca gatcgtaccg gtcgtaaacg gcagcggatt tttttccata
      421 atgacttcca gtgccttttg gtattccccc tgctcaacaa gacgcatata agtcatcaca
      481 tcctgatgga tcgggcactc ctcctgacag ggagcgacaa aacagtcaaa cagcggcagt
      541 tcttttttca gttttcggtt ctgatgcggt ttgacaggtt ttctgtgatg tttgtcaaaa
      601 acagcggcct ctgcgagcac cttcgccttt ttcacatcca caccggcaaa ggaagaaaac
      661 tgcttcccat gaaaagcctt tgcaatctgg ataaaacgcg cataaccgcc cggcttcagc
      721 gcggtcgtag ccattgtgac aggccagatc ccggcatcca cgatctgctc gatattatgg
      781 atatcggctc cgccggaata agagatccgc agccggccgt caaattcttc ggaaagcttc
      841 gccgcaaggg caatcgacaa cggataaaga gcacgtccgg acatatacat ttcctcgccc
      901 ggcagctcgc cggccttgat gtcgaccgga aacgtatttg taattttaac accgaattcc
      961 agtccctttt cggatgcaag cgacatcaga cgagtcagca tcggcacagc atcctcatac
     1021 tgaagatcat ctttaaaatg aaaatcggta aacgaaacat aatcataacc cattttatca
     1081 agcgtttttc ttgcaaattc atagccaaga agcgtcggat tgcatttgat gaacgtatgc
     1141 agctgttttt gtgtcaaaag atacgtggcg atccgttcga tttcctgcgg cggacatcca
     1201 tgcagagtcg acagcgtgat cgaactgcag atctctgccg ggatctgctc gatatcctct
     1261 tttgatacat tggaaaacag ctccgtatga cgcagtgcat attccataca ttctttaaag
     1321 accggcgtat cggaagcatc cttcagcccc tcaataaaat catcgatctt tttcgttttg
     1381 atcccgtcaa gatcatagcc gacactcata ttgaaaatga atccgtcaga tgcaccaagt
     1441 ccaaactcct ttgccatcag atgcatcaga aaccatgcct tgacatattc ctcaaaagcc
     1501 tgcggcacat aaagttccgt cgaccattcc acattatatc cttcatcatc tgccataatg
     1561 cacggcttgc tgaccggaag atcatctccg tccagcttct gcaccgtctt taattcaaag
     1621 aaacggcctc ctgcataata agccgcagcc agattctgag caagctgcgt atgcggtcct
     1681 gctgccggtc cgaacggcgt ttcaagcatt tccccaaaca gggaaagcct ccgttctttt
     1741 tctgcaaaat aagacccatg caggccaaat accgcccctc ctgcctcttt ttctccaaat
     1801 atccagttca gcagctgctc aaaggataat ggtgtcattt tatctcccat agtatgacct
     1861 cactttcatc gttgctgtct atctgctgtc tatctgctgt ttatctgctt ccacaatttt
     1921 tcggattctt cgcgtactcc tgcaagcact gcttcctcgt caagctgtgt cagttttctg
     1981 tcctgcatca gcaccctgcc gtccgcaacg gtcgtcacaa cactccggcc ggtcatccca
     2041 aacagcacat gaccattgat attatccgca ttcatcggag tcagcggaat ataatccgtt
     2101 acgatcacat ccgcacttgc cccttctttt aaaataccga ccggcttttt aaaataacgg
     2161 ccggcaattt tcgcattatt ctcaaacagc atgacaggca cttcacccca tgcggcattc
     2221 gcgtcacata aatgatgctt atgaagaaca tttgccactt tatacgattc tgtcatatca
     2281 tgtgtataac cgtctgttcc aagtcccgtc aagatcccct tggcaaaaat tttcatcgta
     2341 ggcggacagc cgcaggcatt tcccatattc gattccggat tatgcacgac catcgtatcc
     2401 gtttccttca gcacatccat ttcatgatcg tttacataga tacaatgcgc ggtgagcgtc
     2461 ttttctccca gtattccatt atccatcagc cggtcaatga tccgttttcc gtactttttc
     2521 agagaatcgt acagatcctc gatcccttcc gccacatgaa tatgataccc gacaccatcc
     2581 ggcttgtgtg cagcaaagcg ccagcgtttc atccgataat gtaaatgacg catgcagacc
     2641 catcataccg gcgatcatat cggtatcgtc tgcaagcgca tgtttaataa acgcttcatt
     2701 ttcaagaacg gcatccgttc tcttttccat tccgtcccga tccgacactt catagcacag
     2761 acagcttctg atccctgtct cctttgcagc ctcttcgatc ttaaacaaac tgtccctgat
     2821 ggaaccgtag cttgcatgat gatcaaacac cgttgtcaca ccgtttttga tacagtcaat
     2881 ataggtggca agtgcactct gctttgtctg ctccaatgtc agattgcggt cgatcgtcca
     2941 ccacataccg tccagaatat ctaaaaatcc tttcggattg taaccccgga tggaaagtcc
     3001 ccttgccatt gccgagtaga tatgttcatg cgtattaata aagcccggca taatgacgcc
     3061 tcctttggca tcaatcagct gggcatccct gtactcctgc ttcagcgttt cataatcacc
     3121 gatctttttt attacccgat cgtctatgag caccgcaccg ttctccatga atggattgtc
     3181 tttgtccctt gttactactt ttccgtttcc tattatcagc atagaattcc ctcctccgcc
     3241 gtcagaatgg ttgtttttag tttagcactt ataatttctc aatacaataa cttttccttt
     3301 gaaaaacaca aaattcaaaa aaaaagtttg ttgctctaat tttttttgga aaatacattg
     3361 tttttcttat ttttcatgct atgat
//
LOCUS       DAOSYE010000135         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20183_length_3377_cov_3.156532, whole genome shotgun sequence.
ACCESSION   DAOSYE010000135 DAOSYE010000000
VERSION     DAOSYE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20183_length_3377_cov_3.156532"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLU43_03275"
     CDS             <1..324
                     /locus_tag="PLU43_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO27461.1"
                     /translation="ALNSLEVGEGKTSLCGTRAGADMNEGLRINRVKYNKQVIEKPEL
                     DTAGVAFFDGKREDPDLIEQQIFYNAVMNGAELVVKPEQAFVVTQILAAIYRSAKTGK
                     PVFFE"
     gene            365..1336
                     /locus_tag="PLU43_03280"
     CDS             365..1336
                     /locus_tag="PLU43_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO27462.1"
                     /translation="MKLGVFNPVLRSLPFEEACTYFENYGIQMVEIGCGGFPGKSHCD
                     PQVLLQDDEKCQEFQNTLKRHHLEISALSCHGNPLHPNKETAKKFDDDLTDAILMCEK
                     LGVEILNTFSGCPGSDETALKPNWVTCSWPEDFSEILEWQWEEVLIPYWKQKAAFAKE
                     HGVDKIALELHPGFCVYNTKSLLRLRNAVGPEIGANLDPSHLIWQQMDPVEVIRVLGN
                     EHAIFHFHAKDTQIHPYNCRINGVLDTTHYADSRNRSWTFRSIGYGHDLGYWKDIISA
                     LRMAGYDHSISIEHEDGLMSPNEGLVKTIEALKQCMMFEDPGEMYWA"
     gene            1354..2400
                     /locus_tag="PLU43_03285"
     CDS             1354..2400
                     /locus_tag="PLU43_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO27463.1"
                     /translation="MNERKFKLAIVGFGGMGNWHRELIERIEHLSVKGICDIKKERQD
                     YARELGLEVYRSLKDVLEDPEIDIVLCAAPNDVHKEIVIQTLEAGKHIVCEKPAAIHA
                     KDFEEMMAAQKTSRLFVHQNRRWDEDYLTIRKVLDENLLGQVFQIESRVHGSRGIPGD
                     WRGMKEHGGGVVLDWGVHILDQALCMIEGKVKNVYAAVTHVTNELVDDGFMARLMFEN
                     GVEYIAEVGTSNFIPLPRWYVLGCDGTAVIRDWELSGEVVRVTDWNKNDAMPVKTAAG
                     LTKTMAPRTEDTIAAEALPVVHSDIRDYYRNVMAVIDGVEEQKVKNEEVLRVLRLIEA
                     IFVSAKEKQVVDFE"
     gene            2612..>3377
                     /locus_tag="PLU43_03290"
     CDS             2612..>3377
                     /locus_tag="PLU43_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27464.1"
                     /translation="MNSIKEPLLYVQDLSVDFRTAEGTVHAINGLDYELYPGEILGIV
                     GESGSGKSVSSLSAMRLIPDPPGRIVSGQILFQGNDLVKETEKNMEKIRGNQISMIFQ
                     EPMTSLNPILTCGFQIAESLMIHRGLKKKEAMKEALQLMKDVGIADAKKRMHEYPHQM
                     SGGMKQRVMIAMALACEPAILICDEPTTALDVTIQAQILDLIKNLNQENNTSIIMITH
                     DFGVISELCERVLVMYTGKIVEEAPAADIFKDPIHPY"
BASE COUNT         1021 a          612 c          923 g          821 t
ORIGIN      
        1 gcactgaaca gtctggaggt aggtgaagga aaaacctctc tctgcggaac cagagcgggc
       61 gcggatatga atgaagggct tcgcattaac cgggtaaaat acaataaaca agtgatagaa
      121 aaaccggaat tagatacggc cggagtcgca tttttcgatg gaaaaaggga agatccggat
      181 ctgatcgagc agcagatctt ttataatgcg gtaatgaatg gcgcagaact ggtcgtaaaa
      241 ccggagcagg cgttcgtagt tacacagatt cttgcagcaa tttatcgttc ggcaaaaaca
      301 gggaaaccgg tatttttcga ataaaaagaa cttttataat cataaacaaa cggaggaaaa
      361 ataaatgaaa ttaggcgtat ttaatccagt acttagaagc cttccttttg aagaggcctg
      421 tacatatttt gaaaattacg ggattcagat ggttgagatc ggctgcggcg gatttcctgg
      481 aaaatcgcac tgcgatccac aggtgttatt acaggatgat gaaaaatgtc aggagtttca
      541 gaatactctg aaaagacatc atcttgagat cagtgcgctc agctgtcatg ggaatccgct
      601 ccatccaaat aaagagaccg ccaagaagtt tgatgatgat ctgactgatg cgatcctgat
      661 gtgtgaaaag ctgggcgtag aaattttaaa tacattttcc ggctgtccgg gatccgatga
      721 gacggcattg aagccaaact gggtaacctg ctcctggccg gaagatttca gtgagatctt
      781 agagtggcaa tgggaagagg ttctgatccc gtattggaaa cagaaggctg cctttgcaaa
      841 ggagcatggt gtagataaga tcgcactgga gcttcatccg ggattctgtg tatataatac
      901 aaagtccctg cttcgcctgc ggaatgcagt tgggccggaa attggagcaa atctggatcc
      961 aagtcatttg atctggcagc agatggatcc ggtcgaagtc atccgagttc ttggaaatga
     1021 acatgcgatc ttccattttc atgccaaaga tacgcagatc catccataca actgcaggat
     1081 caacggtgtt ttggatacta cacattatgc ggacagccgg aatcgttcct ggactttccg
     1141 ttctattgga tatggacatg accttggata ttggaaagac attatttctg cactgcgtat
     1201 ggcaggttat gaccattcga tcagtattga gcatgaggat ggcctgatga gcccgaacga
     1261 aggtctggta aagacgatcg aagcgctaaa acagtgtatg atgtttgaag atccgggaga
     1321 aatgtactgg gcatagaaga aagggaacac attgtgaatg agagaaaatt taaattagcg
     1381 atcgttggct tcggcggtat ggggaactgg caccgtgaac tgatcgaaag aatcgaacac
     1441 ttatcagtaa aaggaatctg tgatataaaa aaagagcggc aggattatgc cagggaactt
     1501 ggcctggaag tataccggag cctgaaagat gtgctggagg atcctgagat agacatcgtt
     1561 ctctgtgcgg caccaaacga tgtgcataag gagatcgtaa tacagacact ggaagcggga
     1621 aaacatatcg tctgtgaaaa accggcggca attcatgcca aagattttga agagatgatg
     1681 gctgcacaga agaccagccg cttatttgtg catcagaatc gcagatggga tgaagattac
     1741 ctcaccattc gaaaggtcct ggatgagaat ctgcttggac aggtatttca gatcgagtcc
     1801 cgtgtgcatg gatcgcgcgg gattccggga gactggcgcg gcatgaagga acacggcggc
     1861 ggcgtggtgc tcgactgggg cgtccatatt ttagatcagg cactctgcat gatagaaggg
     1921 aaggtaaaga acgtatatgc tgccgttacc catgtgacga acgagttggt agacgatggt
     1981 tttatggcac gtctgatgtt cgaaaatggt gtggaatata tagcggaagt tggtacaagc
     2041 aattttatac cacttccacg ctggtatgtg cttgggtgcg atggtacggc agttatacgt
     2101 gactgggagc tgtcaggtga agtggtgcgt gttacggact ggaataaaaa tgatgccatg
     2161 ccggttaaga cagcagcggg gctgactaaa acgatggctc cacgtacgga agatacgatt
     2221 gcagcagagg cgctgccggt tgtgcacagc gatatccgtg attattaccg aaatgtaatg
     2281 gctgtgatcg acggagtgga agagcagaaa gtgaaaaatg aggaagttct tcgtgtgtta
     2341 cggttgatag aagcgatctt tgtatctgcg aaggagaagc aggtggttga ttttgaataa
     2401 aagacagtgg acagagagcg gtgtttgtta gtttacatta tttcgataaa atttcacaaa
     2461 taatgaaaaa ataatcttga ttttattgac aatgaaaaaa taatctgtta tagtttaaaa
     2521 caatatgaca aaggagcctt ccctggtagt ttgcgggccg gctccttttt acatgagtgg
     2581 caatgaggaa gacggcgata ggagcattat catgaattct ataaaagaac cgctgctgta
     2641 tgttcaggat ctcagtgtgg attttcgcac ggcagaagga acggtacacg cgattaatgg
     2701 gttggactat gaactgtatc ctggagaaat attagggatc gtaggggaaa gcggaagcgg
     2761 caagagtgtt tcctcattaa gtgccatgag actgatcccg gacccgcccg ggcggatcgt
     2821 atccggtcag atcctgtttc agggaaatga tctggtaaag gaaacagaaa agaacatgga
     2881 aaagatcaga ggcaatcaga tctccatgat atttcaggag cctatgacat ccctgaatcc
     2941 gatcttaaca tgcggatttc agatcgcaga atcgctgatg atccatcggg ggctgaaaaa
     3001 aaaggaagcg atgaaagaag cgctgcagct aatgaaggat gtcgggatcg ccgatgcaaa
     3061 aaagaggatg catgaatatc cgcatcagat gtccggcggt atgaagcaga gagtcatgat
     3121 tgcaatggca cttgcttgtg aaccggccat attgatctgc gatgagccta caacagcgct
     3181 tgatgtaacg attcaggcac agatcctgga tctgatcaaa aatctgaatc aggaaaataa
     3241 tacttctatt attatgatca cgcatgattt cggagtgatc agtgaattgt gtgaaagagt
     3301 tttggtcatg tatacaggaa aaatagtaga agaggcaccg gcagcagata tatttaagga
     3361 tccgatccat ccctata
//
LOCUS       DAOSYE010000136         3366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20291_length_3366_cov_2.678949, whole genome shotgun sequence.
ACCESSION   DAOSYE010000136 DAOSYE010000000
VERSION     DAOSYE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20291_length_3366_cov_2.678949"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..949)
                     /locus_tag="PLU43_03295"
     CDS             complement(5..949)
                     /locus_tag="PLU43_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO27465.1"
                     /translation="MKYIETLKESDSISDVYLIKHKQSAVTKNGKTYENLILQDKTGT
                     IDAKIWDPNSSGIEDFDTLDYVQIYGNVNSFNSMLQVSVKRARKCREGEYDPSDYLPV
                     SSKDIETMYQELLGYISGMRSVYLKQLCEAFFVKDESFVKAFKQSSAAKSLHHGFVGG
                     LLEHTLSVTKLCEYYTKAYPMLKKDLLFSVAMLHDIGKVRELSLFPENDYTDEGQLLG
                     HIVIGCEMVGEKIRSIPGFPEQLAGEVRHCILAHHGEYEFGSPKKPALMEAVALNFAD
                     NTDARMEMMTELFSATPETGWLGFNRLFDSNLRKTSGE"
     gene            complement(976..2403)
                     /locus_tag="PLU43_03300"
     CDS             complement(976..2403)
                     /locus_tag="PLU43_03300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOO27466.1"
                     /translation="MKDLDKQPRETHSESHTDHTEAENGGAIRDTVAKENEQASAGAE
                     QNFEFITETIKEKPINKRRMFMKFVCNFCMAVMFGLVACFTFLLVRPYISEWLYPEET
                     KVVTLPADEEPAEPEPVPEPEPEPETVPDTSEDKPETVVTQVVETVEKELEIEDYTLL
                     YQKIDAVAEEAKKSMVTVTGVCSDTDWFMNTYEDNNQAAGLIVTDNGKELLIISRTSV
                     LEGAESIEVTFCNGTMLEGTVKKSDANTGLTVIAVEMSLIDEDTMNQITMATLGSSNN
                     SSLVGSPVIAIGNPLGIADSMAIGQITSNSYVKDMTDTNTQFMTTDIYGSTAASGVII
                     DMDGKVLGIIFQDDTTSDMKNLIKAYAISDLKAKIEKLSNGQDLAYLGIIGTDVTEAA
                     EEMGVPKGAYIKEVIIDSPAMQQGIQNGDVIVKIGTTEITSFTDFKEAMLKCQPGDLM
                     MVTVKRPGKEEYIELSYEVTLGTLE"
     gene            2648..>3366
                     /locus_tag="PLU43_03305"
     CDS             2648..>3366
                     /locus_tag="PLU43_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOO27467.1"
                     /translation="MLRRQRITIARIRPMNSKVLQTLEFHKILEQLSAKASSRPGKER
                     CLLLAPSHDIAHIQKTQLQTKDALTRLFRNSSVSFNGSTDILAAVKRLDKGSSISAGE
                     LQQIASLLENTNRIKAYARKENADVPDDSLDELFSLLEPLTLVSAEIRRCILSEEEIS
                     DDASPDLKKIRRLQKVTNDNIHTRLNHMVNTSCRTYLQDSVITMRNGRYCLPVKSEYK
                     TAVPGMIHDQSATASTFFIEPA"
BASE COUNT          856 a          916 c          658 g          936 t
ORIGIN      
        1 tccattactc cccgctcgtc tttcttaaat tagaatcaaa caatcggtta aagccaagcc
       61 agcccgtttc cggcgtggca ctaaacagct ccgtcatcat ttccatccgg gcatccgtat
      121 tgtccgcaaa attcaatgcc accgcttcca tcagagccgg ttttttcgga gaaccgaatt
      181 cgtattcccc atgatgcgcc aaaatacaat gcctgacttc tccggcaagc tgttcgggaa
      241 agcccggaat cgatctgatc ttttcgccga ccatctcgca gccgatcacg atatgtccca
      301 agagctgtcc ttcatccgta taatcatttt ccggaaacag ggaaagctcc cttacctttc
      361 cgatatcgtg cagcattgcc acactgaaca gcagatcttt tttcagcatt ggatatgctt
      421 tcgtataata ttcacagagc ttcgtcacac tgagcgtgtg ctctaaaagt ccgcctacaa
      481 acccatgatg gaggcttttt gccgcagagc tttgtttaaa ggcttttaca aaactctcat
      541 cctttacaaa aaaggcttcg catagctgtt tcagataaac agaccgcatc ccggaaatat
      601 atccaagcag ctcctgatac atcgtctcga tgtccttaga agaaaccggc agataatcgg
      661 atggatcata ttctccctcc cggcacttcc ttgcacgctt tacactcacc tgaagcatgg
      721 aattaaaact gttgacgttt ccgtagatct gtacataatc aagtgtatca aaatcttcga
      781 tccctgaaga attcggatcc catatctttg cgtcgatcgt tcctgtcttg tcctgcagga
      841 tcagattttc ataagttttt ccatttttcg taacagcaga ctgcttatgc ttgatcagat
      901 aaacatccga aatactgtca ctttctttca atgtctcaat atatttcatt tatccctttc
      961 tctttccatt ataaattatt ccagcgtacc aagcgtgacc tcataggaaa gttcgatata
     1021 ttcctccttg cccggacgct tgactgttac catcatcaga tcacccggct ggcatttcag
     1081 catagcctcc ttgaaatcag taaaggaagt gatctccgtt gttccgatct ttacgatcac
     1141 gtctccgttc tggatcccct gctgcatggc gggggaatca ataataactt ctttgatata
     1201 agcgcccttc ggcacgccca tctcctcggc tgcctccgtg acatctgttc cgataatacc
     1261 aagataggcc agatcctgcc cgtttgaaag cttttcgatc tttgccttca ggtcggagat
     1321 cgcatacgct ttgatcagat ttttcatgtc ggaagtcgta tcatcctgaa aaatgatacc
     1381 aagcaccttg ccgtccatgt caatgatcac accgcttgcc gccgtgcttc cgtaaatatc
     1441 cgtcgtcatg aattgtgtat tggtatccgt catatccttt acataggaat tagatgtgat
     1501 ctggccgatc gccatggaat ctgcaatacc aagcggattt ccgatcgcaa tgaccggact
     1561 tcccacaagg gaactgttat tggagcttcc aagcgttgcc attgtgatct gattcatcgt
     1621 atcttcatct atcaggctca tttccacagc aatgaccgta agtccggtat ttgcatccga
     1681 tttctttaca gtcccttcca gcatcgttcc attacagaaa gtaacttcga tcgactccgc
     1741 gccttccagc acgctcgttc tggaaatgat cagcagttcc ttgccgttat cagtaacgat
     1801 caggccggct gcctgattat tatcctcata tgtattcata aaccagtcgg tatcagaaca
     1861 gaccccggtg accgtgacca tggatttctt cgcttcctcc gccactgcat ctattttctg
     1921 atataacaag gtatagtctt ctatttccag ctctttttcg accgtttcca cgacctgtgt
     1981 cacaactgtc tccggcttat cctccgatgt gtccggaact gtttcaggtt ccggctccgg
     2041 ctccggcaca ggttccggtt cggccggttc ctcgtcagcc ggcagtgtta cgaccttggt
     2101 ctcttccgga taaagccatt cactgatata gggtctgaca agtaaaaacg taaaacaggc
     2161 aaccagacca aacatgaccg ccatgcaaaa attacagacg aacttcataa acatacgcct
     2221 cttattgatt ggtttttcct tgattgtctc cgtaataaac tcaaagttct gctcagcacc
     2281 tgccgaagcc tgttcgtttt cctttgcaac ggtgtcccga atggctcccc cattttcggc
     2341 ttccgtatga tccgtatgtg attcggaatg tgtttcccgt ggctgcttgt ccaagtcttt
     2401 cattcggcgt ctccttctcc caatgtgctc ccttaagatt attccctccc gatcccatgt
     2461 acgactttta ttcgtaagac aaaccggcag atgcagagcc aacccccatt gactatactg
     2521 ccgatttatt tattatacta aacttaggga aaacatacaa caaaaacata cgctccgatt
     2581 gctaaatgag acagattctg ctacactaag catagcgctg acgcttaata tacaggcaca
     2641 gcaatccatg ctgcgccggc agcgaatcac gatcgcaagg ataagaccga tgaacagcaa
     2701 agtactgcaa actcttgaat ttcataaaat attggaacag ctctccgcta aagcatcctc
     2761 gcggcccgga aaagagcgct gcctgctgct tgctccgagt catgatattg ctcatataca
     2821 aaaaacacag ctgcagacaa aggacgcgct tacaagactg ttcagaaaca gctctgtttc
     2881 ctttaacggc agcacagata ttcttgccgc tgtaaaacgg ctcgataaag gcagcagtat
     2941 ttccgcaggt gagctgcaac agatcgcttc tcttctggaa aacacgaacc gtatcaaggc
     3001 ctatgccaga aaagagaatg cggatgttcc cgacgattcg ctggacgaac tgttttcttt
     3061 actggagccc ctgactttgg tgtcggcgga aatccggcgc tgcattttgt cggaagaaga
     3121 aatcagcgat gacgccagtc ctgacttaaa aaaaatccgc cgtcttcaga aagtaacgaa
     3181 tgataatata catacacgac tgaaccacat ggtaaacacc tcctgccgca cttatctgca
     3241 ggacagcgtt attacgatgc gcaacggcag gtactgcctt cctgtcaaat ccgaatacaa
     3301 aacagccgta cctggtatga tccatgatca gtcggctact gcctccacat tttttatcga
     3361 accggc
//
LOCUS       DAOSYE010000137         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20385_length_3353_cov_2.905094, whole genome shotgun sequence.
ACCESSION   DAOSYE010000137 DAOSYE010000000
VERSION     DAOSYE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20385_length_3353_cov_2.905094"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLU43_03310"
     CDS             <1..773
                     /locus_tag="PLU43_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOO27468.1"
                     /translation="SFHRFAKLLPEDGVLIINADIADYEEITDGLSCRTITFGMEHGG
                     DYRAEEIVFDENACASFTVLKADAPFTRITLHVPGRHNIANALAAIALADELSVSSAI
                     MQKGFSTFCGTERRFEYKGMLGGVTVIDDYAHHPTEIRATLHTALGYPHNKIWCVFQP
                     HTYSRTKAFFHEFAAALSLADKVVLADIYAARETDTLGISSMKLADEIKKNGTDAYYF
                     PSFDEIENFLLQNCIHGDLLITMGAGDIVKVGENLLGL"
     gene            1079..2200
                     /locus_tag="PLU43_03315"
     CDS             1079..2200
                     /locus_tag="PLU43_03315"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOO27469.1"
                     /translation="MSQITLCGDFYSDATVVSNQFIDYYMKDANDAQIKIYLYLLRCI
                     SGAKSVSVSLIADCFNYTEKDVVRALKYWEKQKLISLIFNSTKQLTGIKLLPPIKCCI
                     DGSHSDDTAYSVSVPAAGEPALTAQDETVPAKVFKLPAKPSYSLDKLAAFKNRDDISQ
                     LLFIAEQYLNKTLSSNDISSLLYMYDSLGFDAELIEYLIEYCVNNRKKSIRFIETVAN
                     SWAEAGIRTVEDAKIHTSSFSKEIYEIFKALGLNTSRTPVEPELAYMHKWLDSFGFNM
                     DIITEACSRTIMAIHSPSFEYTDTILSNWAAAGVRHVHDIAKQDEQFRNTKSSVKSIP
                     AAKGTAAGTAKDNAVSNKFNSFSQRTYNYSELEKEILSN"
     gene            2241..3215
                     /locus_tag="PLU43_03320"
     CDS             2241..3215
                     /locus_tag="PLU43_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO27470.1"
                     /translation="MALSNSQYDAIMRTYEETKFKNRHLMEERRDEVYQLIPEYQSLD
                     SSIASLSVEQGKKLIMGDPDALIALRKKLGEMSRRKKQLLSEHGFPENYLEPIYSCPD
                     CRDSGYINGQKCHCLKQAIIRTLYMQSNMESVLEKENFDKLSYDYYNDSEVELMRSII
                     GDCKSFVKFFDRSYENLLFYGAVGVGKTYLTNCIAKDLLENGHSVIYFTSFQLFDTLA
                     KYAFRSADLSENIANIHEDIFSCDLLIIDDLGTETTNSFVSSQLFLLLNERDLRRKST
                     IISTNLSLEMLANRYSERNFSRIFGNYKMLKPDIQDIRIKMKKLKNRK"
     gene            3227..>3353
                     /locus_tag="PLU43_03325"
     CDS             3227..>3353
                     /locus_tag="PLU43_03325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281834.1"
                     /note="Catalyzes the formation of PRPP from ATP and ribose
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO27471.1"
                     /translation="MPNREEKRNLETIPVGSLRMIPLEGCRELGEKIDKYLIKWRA"
BASE COUNT         1005 a          715 c          696 g          937 t
ORIGIN      
        1 gttcctttca ccgctttgcg aagctgctgc cagaggatgg cgtactgatc attaatgcgg
       61 atattgcgga ttatgaagaa atcacagacg gtctttcctg caggactatt acattcggca
      121 tggaacacgg cggcgattac cgggcggaag agatcgtttt tgatgaaaac gcctgcgctt
      181 cctttacggt tttaaaagca gacgcaccct ttacgaggat cacgcttcac gtccccggca
      241 ggcacaatat cgccaatgca cttgcagcga tcgccctggc agacgaactt tcggtctcct
      301 ccgccattat gcagaaagga ttttccacat tctgcggaac ggaacgaaga tttgaataca
      361 aaggtatgct cggcggagtt acggtcattg acgattacgc acaccatccg actgagatcc
      421 gtgcaaccct gcacacagca cttggatatc ctcacaataa aatctggtgt gtatttcagc
      481 ctcataccta ttcccgtaca aaagcttttt ttcatgaatt tgctgcggca ctttcacttg
      541 cggacaaagt agtcctcgca gatatttatg cggccaggga aacagacacg ctcggaatca
      601 gttccatgaa actggccgat gagatcaaaa aaaacggaac cgatgcttac tactttccgt
      661 cttttgatga aattgaaaat tttttattac aaaattgtat tcacggtgac ctgttgataa
      721 ctatgggagc cggagatatt gtaaaagtag gcgaaaacct gcttggtctc taagttgtcc
      781 acacaatcca cagttttttt taatttcctt cttaaaaaaa ctgtggattt tcaatttgaa
      841 gaaaatctta aaactgtttc cggcaagctt tgaaattgtg cagtttttta aaaaacaaag
      901 gggagacaat gctgttttca tccacacaat ccacagtgtc cacaatctcg caaacccgct
      961 gttttactga cttttttaca aaaaatactg ttttcttttt ttcgtttgta tgctataatt
     1021 tatcccggta gcagggagga atctttcctg ttccaatatg aaatgaatgg gtgagataat
     1081 gagtcagatt actctctgcg gagattttta ttctgatgcg acggttgtgt ccaatcagtt
     1141 tattgattat tacatgaagg atgcaaatga cgcgcagatc aaaatatacc tgtacctgct
     1201 gcgctgtatc agcggagcaa agtctgtttc cgtctctctg atcgcagact gttttaatta
     1261 tacggaaaaa gatgttgtac gtgcattaaa atactgggaa aagcaaaaac tgatttccct
     1321 gatttttaac agcacaaaac agctgaccgg cattaaactg cttccgccga tcaaatgctg
     1381 tatcgacggc agtcattccg atgatacggc gtattcggtt tccgtgcctg ccgctgggga
     1441 acctgctctt accgcgcagg atgaaacggt ccctgccaag gtatttaaac ttcccgccaa
     1501 gccttcttat tctttggaca agcttgctgc ctttaaaaac agagatgata tttcacagct
     1561 cctttttatt gcggaacaat acctgaataa aacattaagc agcaatgata tttcttcgct
     1621 gttatatatg tacgattcgc ttggctttga cgctgaactg atcgaatact taatagaata
     1681 ctgtgtcaac aacagaaaaa aaagtatccg ttttatagaa accgttgcta attcctgggc
     1741 cgaagccggt atccgtaccg ttgaagacgc aaaaatacat acttccagtt tttcaaagga
     1801 aatttatgaa atatttaagg ctctcggttt aaatacaagc aggacacctg tcgaaccgga
     1861 actcgcctat atgcataaat ggctggatag ttttggtttc aatatggata ttattacaga
     1921 agcctgcagc agaacgatca tggcgattca cagtcccagc tttgaatata ccgataccat
     1981 tttgtcaaac tgggcggcag ccggcgtcag gcacgtgcac gatattgcaa aacaggacga
     2041 acagtttcga aatacaaaat cctccgtgaa atccattccg gccgcaaagg gaaccgctgc
     2101 gggtactgca aaagacaatg ccgtatccaa taaattcaac agcttttcac agcggacata
     2161 taattattcg gagttggaaa aagaaattct cagcaactga cagaactatt taaaacggcg
     2221 gaatcaataa ggaaacagct atggcactca gcaattcgca gtatgatgcg atcatgagaa
     2281 cttacgaaga aacaaaattt aaaaacaggc atttgatgga agagcggcgc gatgaagtct
     2341 atcagctgat cccggaatat cagtcgctcg attcctcgat tgcctctctt tcggtcgagc
     2401 aggggaaaaa actgattatg ggtgatcccg atgctcttat tgctttgcga aaaaaactcg
     2461 gcgaaatgag cagacgtaaa aaacagctgc tttccgagca cggcttccct gaaaattatc
     2521 tcgaacctat ttattcctgt cctgactgca gggattccgg ttatattaac ggtcaaaaat
     2581 gtcactgcct gaaacaggcg atcatccgga cactttatat gcagtccaat atggaatccg
     2641 ttttagaaaa ggaaaatttt gataagcttt cttatgatta ttacaatgat tccgaagtgg
     2701 aactgatgcg ttcgatcatc ggcgactgca aatcttttgt caaatttttt gaccgctcct
     2761 atgaaaatct tctgttttac ggtgcagttg gtgtcggcaa gacttatctg acaaactgca
     2821 ttgcaaaaga tctgctggaa aacggacatt ccgtaatcta ctttacatcc tttcaattat
     2881 ttgatacact tgcaaagtat gcttttcgtt ctgccgatct gtcggaaaac atcgcaaata
     2941 tccatgaaga tattttttca tgcgacttgc tgattataga tgatcttggc acggaaacga
     3001 ctaactcctt cgtttcttcc caattatttc ttttgctgaa tgaaagagat ctgagaagaa
     3061 aatcaacgat catttcaaca aatctttctc ttgaaatgct ggcaaaccgg tattcggaac
     3121 gaaatttttc cagaatattc ggtaattata aaatgctcaa accggatata caggatatcc
     3181 gtataaaaat gaaaaagctg aaaaacagaa agtgaggtca ggcattatgc caaatcgcga
     3241 agaaaagcgg aatctcgaaa cgattcccgt aggttcctta cgtatgatcc ccctggaagg
     3301 atgcagggaa ttaggggaga agatcgacaa atatcttatc aaatggagag cag
//
LOCUS       DAOSYE010000138         3351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20408_length_3351_cov_2.742718, whole genome shotgun sequence.
ACCESSION   DAOSYE010000138 DAOSYE010000000
VERSION     DAOSYE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20408_length_3351_cov_2.742718"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..72
                     /locus_tag="PLU43_03330"
     CDS             <1..72
                     /locus_tag="PLU43_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO27472.1"
                     /translation="TEQKIKSMTVETFGVDYPEPQKA"
     gene            347..1192
                     /locus_tag="PLU43_03335"
     CDS             347..1192
                     /locus_tag="PLU43_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO27473.1"
                     /translation="MKLQIILLKIDKLNHEEERICLARHMRDERPEWELQDFDCYAGE
                     GEKAFLITDSRSAADQALKKNMGFAVYLNETGKAGYFPEALYCIDDLSVLPILQIERM
                     YLRYYGRPWTILETKRCILREITTDDVDALFRIYEDGEVIRYMDGLSESREEEIAFIR
                     EYAEKQYRFYEYGMWVVIRKEDGSLIGRAGIEGRAGFEDAEIGYVIAADCRRRHYARE
                     VCLAVLAYAQEVLGMERLNAFVREENTASKALLHSLGFLAAETAVLQGTLHERYVWKS
                     DELAD"
     gene            1294..2283
                     /locus_tag="PLU43_03340"
     CDS             1294..2283
                     /locus_tag="PLU43_03340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO27474.1"
                     /translation="MVRLAIVVPCFNEEAVLPQSVSELVRIMQELAEKRKISEDSFLL
                     FVDDGSRDRTWELIEKFHKENRFVSGLKLAGNVGHQNALLAGLMSVKDEIDVSISIDA
                     DLQDDIGVIGEMIEKYLSGNDIVYGVRSSRKTDTFFKRATAEGFYKFMRLLGVKSVYN
                     HADFRLMSRRALDGLSEYKERNLFLRGIVPMIGYETDCVYYERRERKAGESKYPLKKM
                     LGFAFDGVTSFSIKPMNFIMATGIFMILLTIAAGIYALVSYSTGNVVSGWTSLILSLW
                     FIGGMVLFSLGIVGQYIGKIYIEVKERPRYLIEKQLLHEEETRPVMEETAHEK"
     gene            2273..3136
                     /locus_tag="PLU43_03345"
     CDS             2273..3136
                     /locus_tag="PLU43_03345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27475.1"
                     /translation="MKNKEITGRTMQDIGMCILLGALLVSAIVLANGGTELYAENLIM
                     LLGIFISVLFAGFRLVSAAIVIACIETVSFAAFKIYLFFVTGEQIKGSSYFWILLPGL
                     AVAGIVLFVNGIKKLQLERDVLEKQVEELVMIDPLTGFYNLRSMFMDIQTQISYAERN
                     KNAVSLMILKLRYDRELQSVLKKQQYNEVILRLAKYVYDTVRLEDRVYSIDREGSLGI
                     ILTCNKKGTELVEGRIRERLSHPQAFAGIATSPIRVEVKIGCLEYKKAEFQRDAKLFK
                     KQVEEEVEYDI"
     gene            3173..>3351
                     /locus_tag="PLU43_03350"
     CDS             3173..>3351
                     /locus_tag="PLU43_03350"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO27476.1"
                     /translation="MKYFITGGAGFIGSNMTDRLLCEPENEVVVYDNFSTGRREFLAE
                     ALKNPRFHLIEGDNL"
BASE COUNT          979 a          543 c          911 g          918 t
ORIGIN      
        1 acagagcaga aaatcaaatc catgacagtg gagactttcg gagtagacta tccggaaccg
       61 cagaaagctt gatgatatgt caggggtatc tcactgcgtg agaattctcc ctcgacttgt
      121 tcatgatttg ttcataaatc attttaaaaa acttcattta cataacattg tttatccatt
      181 tatgtgaact atactaagta cataaacaac agacgacaaa ctcatagtaa cattactatt
      241 taaatacttt ttcataataa atgccaagtg accaggtcac ttggcatcct cccttttaca
      301 gggagttttt tcatttcata aaaaatacgc acagggaaaa taaagaatga aactgcagat
      361 tattttgtta aagatcgata aactgaacca tgaggaagaa cgcatatgtc tggctcggca
      421 tatgcgggat gaaagaccgg aatgggagct gcaggacttt gactgttatg cgggtgaagg
      481 agagaaggca tttctgatca cggacagcag gtctgccgca gatcaggcac tgaaaaagaa
      541 tatgggtttt gcggtttatc tgaatgaaac gggaaaggca ggatattttc cggaagcatt
      601 gtactgtatc gacgatttgt ctgttttgcc gattttacaa atagaaagaa tgtatctgcg
      661 ttattatgga cggccatgga cgatccttga aacgaaaaga tgtattctgc gtgagatcac
      721 gactgatgat gtggatgcgc tgtttcgtat ttatgaagac ggggaagtta tccgctatat
      781 ggacggactg tctgaaagca gagaagaaga aattgctttt atcagggagt atgcagaaaa
      841 acagtatcgg ttttatgagt atggaatgtg ggtagttatc agaaaagaag acggaagcct
      901 gatcggacgt gccgggatcg agggaagagc cggatttgaa gatgcggaga tcgggtatgt
      961 gatagcggcc gactgcaggc gcaggcacta tgccagggag gtttgcctgg ctgtacttgc
     1021 ctatgcgcag gaagtgcttg ggatggaacg gctgaatgct tttgtcagag aggaaaatac
     1081 ggcatcgaaa gctttactgc attccctcgg gtttctggct gcagagacag cggtattgca
     1141 gggaacactg catgaaagat atgtatggaa gtctgatgaa ttggcggact gacaaggaat
     1201 cactggattt tgtaaataat gtatgatata atgctcacgg atgagttttg cacgtgtatg
     1261 gcacggcaga tgatatttca gagggatcaa aggatggtaa gactggcaat agtagttccc
     1321 tgttttaatg aggaagctgt tctgccgcag tctgtttccg agttagttag aataatgcag
     1381 gagctggctg aaaaaagaaa aatttcagaa gacagctttc ttttattcgt ggatgacggg
     1441 agcagggaca gaacgtggga attaatagag aaatttcata aggaaaatcg ttttgtatcg
     1501 ggacttaagc ttgccggaaa cgttgggcat cagaatgctc tgcttgccgg tctgatgtcg
     1561 gtgaaggatg agatcgatgt cagcatttcg atcgatgcgg atctgcagga tgatatcggt
     1621 gttatcgggg aaatgattga aaaatatctt tccggaaatg atatcgtata tggtgtacga
     1681 agcagcagaa agacggatac ttttttcaaa agggcgacag ccgaggggtt ttataaattt
     1741 atgaggctgc tcggtgtgaa aagcgtttat aaccatgcgg attttcggct gatgagcagg
     1801 cgtgcgcttg acggcttatc ggaatacaaa gagagaaatc tttttctgcg gggaatcgtg
     1861 ccgatgatcg gatacgagac agactgcgtt tattatgaac gcagggaacg caaggccgga
     1921 gagtcaaaat atccgttaaa aaaaatgctg ggatttgcat ttgacggcgt gacttccttt
     1981 agtatcaaac cgatgaattt tattatggct acgggaatct ttatgattct tcttacaatt
     2041 gcagcgggaa tctatgcgct tgtatcttat tcgaccggaa atgtcgtaag cggctggact
     2101 tccctgattt tatccttgtg gtttattggc gggatggtgt tgttttcact cggaatagtg
     2161 ggacagtata tcggcaagat ctatatagag gtaaaggagc ggccaaggta tctgatcgaa
     2221 aagcagctgc tgcatgagga agaaaccaga ccggtcatgg aggaaacggc gcatgaaaaa
     2281 taaagaaatt acaggaagaa ccatgcagga tatcggaatg tgtatattgc tcggagcatt
     2341 actggtcagt gcgatcgtac tggcaaacgg gggcacagag ctgtatgctg aaaatcttat
     2401 tatgctgctt ggtattttta tctccgtgct gtttgcggga tttcgccttg tcagtgcggc
     2461 aattgtgatt gcgtgtatcg aaacggtttc ttttgcggcg tttaagatct atttgttttt
     2521 tgtaacgggt gagcagatca aagggagttc ctatttctgg atactgttac cgggacttgc
     2581 cgtggcaggg atcgtgctgt ttgttaatgg aatcaaaaaa ttgcagctgg aaagggatgt
     2641 gctggagaag caggtagagg agcttgtcat gatagatcca ttgacaggat tttataatct
     2701 gcgaagtatg tttatggata tacagacaca gatctcctat gcggaacgta ataaaaatgc
     2761 agtatctctg atgattttga agctgcgtta tgacagggag cttcaaagcg tactaaagaa
     2821 gcagcagtat aatgaggtga tccttcgcct tgcgaaatat gtatatgata cggtcagact
     2881 ggaggacagg gtatacagta ttgataggga aggttctctt ggtatcattt taacctgcaa
     2941 taagaagggg acggaattag ttgaaggcag gatccgtgaa aggctttcac atccgcaggc
     3001 ttttgccggt attgcgacct caccgatccg tgtggaagtc aagatcggct gtctggaata
     3061 taaaaaggca gagtttcaaa gagatgccaa gctgtttaaa aagcaggttg aagaagaagt
     3121 ggaatacgat atttagactg ctgtatgcat aatttaggaa agaggataag gaatgaagta
     3181 ttttattacg ggcggagccg gatttatcgg gtctaacatg acagacaggc ttttatgtga
     3241 gccggaaaac gaagttgttg tttatgataa tttttctacg ggacgcaggg aatttcttgc
     3301 agaagcatta aaaaatccgc ggtttcatct gatagaagga gacaatctgg a
//
LOCUS       DAOSYE010000139         3348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20444_length_3348_cov_3.159429, whole genome shotgun sequence.
ACCESSION   DAOSYE010000139 DAOSYE010000000
VERSION     DAOSYE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20444_length_3348_cov_3.159429"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLU43_03355"
     CDS             complement(<1..491)
                     /locus_tag="PLU43_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27477.1"
                     /translation="MLRNALPEIVTQTVPGPKSKELLERRREALPKALGSGIYPICIS
                     RGEGAMLEDLDGNRFLDWIGGVGVLNIGYSRPEVVAAVQKQAENYFHTIINIVTHENY
                     IALAEKLNQIVPVKGEVKKTYFANSGAEADENAIKIAKAYTKRSNVICFSGAFHGRTN
                     LTMA"
     gene            complement(528..1880)
                     /locus_tag="PLU43_03360"
     CDS             complement(528..1880)
                     /locus_tag="PLU43_03360"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HOO27478.1"
                     /translation="MSFEQVSKDLEKVIGYIHSEELSEEEKQEMTKETVEYFDTYVSP
                     GWLKYRKSVSTNSAVLEWKDHDGVCEGLYHEEFIDCLGGFGIYTCGHRNPYILDTVKA
                     QLDHQALHSQELLDPLRGYLAKAVADITPGDLEKCFFTNGGAEAVEMALKLARIATGG
                     RWYISTVGAFHGKSMGAISMGGKSTYRVPYTPMVQQVQHVEFGNAEDARKAVRNLVAV
                     GEKVAAIIVEPIQGEAGVVIPPAGYLKELRSICDEYNVALIFDEIQCGMGRTGTMWRC
                     EAEDVVPDIMTFGKSFGGGIMPITGIICRPHMWTQELIDNPWLLGSPTFGGNPVCCSA
                     ALATIKYMIDNDIPGQCKEKGDYLIAGLQSLQEKYPTAINDVRGIGLMLAVEFVTSEV
                     GYNVAKNLFARGVMTAGTLVNAKCIRFEPAAVITKEQIDAVIQRMDESLAETVKEFSL
                     "
     gene            complement(2064..>3348)
                     /locus_tag="PLU43_03365"
     CDS             complement(2064..>3348)
                     /locus_tag="PLU43_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO27479.1"
                     /translation="IAEITREEIEGENVISAKGKIVCPGFIDPHAHVEGNDACAAVMA
                     AMGVTSVVNGNCGNSPEHLEEFLLQQEKQGFLINQFEQVGHTTLRNLAGNTDRYAAST
                     PEQIAKMKQLAAEAMESGVYGLSFGLEYVPGSSFEEVIELAKVAAAYGKVVSIHTRKD
                     CYAGLSSLQEAIDICRLTGAAVNVSHLTYMFGLGMAAEAIRMIELAMAEGLDVSVDSG
                     LYSAFATEIGSAVFDEDCFEKWHSDYSCIVAGTGKYRGQRLTKEMFYDLRNNYPFESG
                     IGMVGKEYEVYEILEHPYVMVGTDAGTQYDNGRPGHPQDAGTYPKFFKQMLREQNRIS
                     LIDAVDRCTYQVAKRFHIPNKGFLAKGADADLVVFDLSKIEDKAQYPCYGATDTRPEG
                     ITAVIIGGTLAVEGKIVNEIRPGKMYRDKAEPWQW"
BASE COUNT          788 a          950 c          640 g          970 t
ORIGIN      
        1 gccatagtta aatttgttct tccatgaaaa gcgcctgaaa aacagatcac attgcttcgc
       61 tttgtataag cttttgcaat cttgatcgca ttttcatctg cttccgcgcc ggaatttgca
      121 aagtaggtct tctttacttc tccctttacc ggaacgatct gattcagctt ttccgccaat
      181 gcaatataat tctcatgcgt aacgatattg atgatcgtat gaaaataatt ctcagcctgc
      241 ttttgtacgg cagcgaccac ctccggtctg gaatacccga tattcaagac tcctacaccg
      301 ccgatccagt ccaggaaccg gttcccgtca agatcttcca gcatggctcc ttctcctctt
      361 gaaatacaga tcggataaat accgcttcca agtgctttgg gcagtgcctc cctgcgcctt
      421 tcaagcagct cttttgactt cgggcccgga acagtctgcg tgacgatttc gggcaatgca
      481 tttctcagca taataacatc tcctcgcata atattttgta gtatccttta taatgaaaac
      541 tctttcacgg tctccgcaag agactcatcc attctctgaa tcactgcatc gatctgttct
      601 tttgtaatga ccgcagccgg ttcaaaacgg atgcattttg catttacaag cgttccggcc
      661 gtcataacgc ctcgcgcgaa cagatttttt gccacgttgt atccgacttc gcttgttacg
      721 aactctactg ccagcatcag accgatcccg cgtacatcgt tgatcgccgt cggatatttt
      781 tcctgtaatg actgaagacc tgcaatcaga taatcacctt tttccttaca ctgtcccggg
      841 atgtcgttgt cgatcatata cttgatcgtg gcaagcgctg ccgaacagca gaccggattg
      901 ccgccgaagg taggtgaacc gagaagccat ggattatcga tcagttcctg tgtccacata
      961 tgaggacggc agataattcc cgtaatcggc ataataccgc cgccgaatga tttcccaaaa
     1021 gtcataatat caggcacaac atcttccgct tcacatctcc acatcgtacc ggtccgtccc
     1081 atgccgcact ggatctcgtc gaaaatcaat gccacattat attcatcgca aatactgcgc
     1141 agttctttta aatatcctgc aggcggaatg accacgccgg cttctccctg aattggttcc
     1201 acaatgatcg cagccacttt ttcgcctacc gcaacaagat tacgaaccgc tttccttgca
     1261 tcctccgcat ttccgaattc cacatgctgt acctgctgga ccatcggcgt ataaggaacc
     1321 cgataagtac tctttcctcc catggagatc gctcccatgg attttccgtg gaaggcgcct
     1381 actgtagaaa tataccatct gccgcctgtt gcgattctgg caagcttgag tgccatttca
     1441 acagcttcag caccgccgtt cgtaaaaaag cacttttcca gatctcccgg tgtaatatct
     1501 gccacagctt ttgccagata tcctctcagc ggatcaagca gctcctgaga atgcagcgcc
     1561 tggtgatcca gctgtgcttt tacggtatcg agaatatatg ggtttctgtg accgcatgtg
     1621 tagattccaa aaccgcccag gcagtcaata aattcttcat gatataatcc ttcacaaaca
     1681 ccgtcatggt ccttccattc aagcactgcg gaattcgtag atacggactt tctgtatttc
     1741 agccatccgg gactgacata ggtgtcaaaa tattcgaccg tttcctttgt catttcctgc
     1801 ttttcttctt cagacaattc ctcgctgtga atatatccga ttactttttc cagatccttg
     1861 cttacctgtt caaaactcat gatagttcct ccttttgttt ctctatttga taataaatga
     1921 tgccatatga caaaacggaa cgacctatgc gaccgctccg ttgctctata ctactacttt
     1981 acgctccgga cttattgaaa acaattactt gcggatacca tttttatact tcttttgaat
     2041 aacatatgct tcttttatgc taattaccac tgccaaggtt ctgctttatc cctgtacatc
     2101 ttccccggcc tgatctcatt tacaatcttg ccttccactg cgagagttcc tccgatgatc
     2161 actgctgtta ttccttccgg ccttgtatcg gtagccccat agcagggata ttgtgccttg
     2221 tcttcgatct tcgacagatc aaacactacc agatcggcat ccgctccttt agcaagaaag
     2281 cctttattcg gaatatggaa ccgtttcgca acctgatacg tacagcgatc caccgcatcg
     2341 atcagcgaga tcctattctg ctcccgcagc atctgtttga aaaatttcgg ataggtgccg
     2401 gcatcctgcg gatgacccgg cctgccgtta tcatactgcg tacctgcatc cgttccgacc
     2461 attacatacg gatgctccag tatctcataa acttcatatt ctttccctac catgccgatc
     2521 ccgctttcaa acggataatt gttcctaaga tcataaaaca tttccttagt cagccgctgt
     2581 ccgcggtact tgcctgttcc ggccacaata cagctgtaat cgctgtgcca tttttcaaaa
     2641 caatcctcat caaagactgc gctgcctatt tctgttgcaa acgcactgta cagaccgctg
     2701 tccacagata cgtcaagtcc ttcggccata gccagctcga tcatacggat cgcttctgcc
     2761 gccatgccga gaccgaacat atacgtaaga tgtgaaacat ttacggcagc ccctgtcagt
     2821 ctgcagatat cgatcgcctc ctgcagggag gaaaggcccg cgtagcaatc cttcctcgta
     2881 tggatcgaaa cgaccttgcc gtatgcggct gctacttttg ccagctcgat cacctcttca
     2941 aacgaactgc cgggaacata ttccagtccg aaagacaggc cgtatacacc gctttccata
     3001 gcctctgcgg ccaattgctt catctttgcg atctgctccg gcgttgaagc ggcatagcgg
     3061 tctgtgttcc ctgccagatt tctcagcgtc gtatgtccta cctgttcaaa ctgattgatc
     3121 aaaaatccct gcttttcctg ctgtaaaaga aattcttcca gatgctccgg tgaatttccg
     3181 cagttgccgt ttacgaccga cgtcactccc atagctgcca taactgcagc acaggcatca
     3241 tttccttcca catgcgcatg cggatcgata aatccgggac atacgatttt tccctttgca
     3301 gagatcacat tctctccttc gatttcttct cgtgtaattt ccgctatt
//
LOCUS       DAOSYE010000140         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20460_length_3346_cov_3.044667, whole genome shotgun sequence.
ACCESSION   DAOSYE010000140 DAOSYE010000000
VERSION     DAOSYE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20460_length_3346_cov_3.044667"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>440
                     /locus_tag="PLU43_03370"
     CDS             <1..>440
                     /locus_tag="PLU43_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOO27480.1"
                     /translation="RIIGEDLFLYNRCKASLWGAGQEYLSAPRLDDVQCAFASLTGFL
                     RAVPADSIPVLAVFDNEEVGSSTRQGASSTFLKDTLLRINAALGRNMEDYFCAISESF
                     MISADNAHAFHPNYTDKADPVNHPVMNGGIVIKYSADQKYTTDGV"
     assembly_gap    441..450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <451..723
                     /locus_tag="PLU43_03375"
     CDS             <451..723
                     /locus_tag="PLU43_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOO27481.1"
                     /translation="AAVCKILCEKADIPYQIFVNHSDLPGGSTLGSLSGNQVAVSTAD
                     VGIAQLAMHSPYETCGVKDTEYMVRLAQELFSSSVCQDGNERYRII"
     gene            871..1293
                     /locus_tag="PLU43_03380"
     CDS             871..1293
                     /locus_tag="PLU43_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27482.1"
                     /translation="MDDEWYEDEGEERERLHRIRRAERLKRMRQEKRRQQRLRRFLGK
                     IVPRLLAGTAVVLLLVFGIRKIVRTADGNESGQAAAQTVSQETEAATVKIDETVSDNS
                     AEESSSDAGEETGQPDYSAEKTEYTLPFGDDIVSTQAI"
     gene            1294..2040
                     /locus_tag="PLU43_03385"
     CDS             1294..2040
                     /locus_tag="PLU43_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOO27483.1"
                     /translation="MDTETNTILAQRDADAVISPASMTKILTLLVAAEHVTDLDDTFT
                     ITLDITDYSFENDCSNVGFSENETVTVRDLFYGTILPSGADAAVGLATYVAGSQEAFV
                     ELMNEKLDELGLSDTAHVTNCVGIYDEDHHCTVYDMAMILEAAMDNELCRTVLSAHTY
                     TTSATEEHPEGITISNWFLRRIEDKDSAGTVICGKTGYVVQSGNCAASYAEDSDGNGY
                     ICVTVNSTSSWRCIYDHVAIYKQFLSAAGG"
     gene            2144..>3346
                     /locus_tag="PLU43_03390"
     CDS             2144..>3346
                     /locus_tag="PLU43_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO27484.1"
                     /translation="MLATLIPFFDKNMKVCAYSLFSQKQNLLLNPVLLGSGRNDGAAS
                     VNGLEIIQNMGIDTLSPDTDIFVPVGNIAVFSDIESQCSVPHSRLVLLFDNSVMNEEI
                     YLKRFTQLKEEGYKLAIRKLPISSFEESRELIRLMDYIILDCKKVNVMKAKIYFGHLF
                     PHIKICVGNIENQEKFDEVKNDESFWLFEGEFYRIPVTKGQTDVAPLKANYLELLKMV
                     NDADFELTKAADVIGRDTALVLELLEMVNRMSVNSGITSIRHAAAMLGQKELKKWINT
                     AVTKELCTDKPSEITRISLLRAKFAEQLAPIFGLAAKDSELFLMGLFSVLDLILNLPM
                     EDALKKINVSKDIYHALVSHAGEFAKVLDFMLQYEYANWQEVSRQMIIMNIEMSAVYD
                     AYISSIKWY"
BASE COUNT          945 a          593 c          878 g          920 t
ORIGIN      
        1 cgaattattg gagaggatct gtttttatat aaccgttgta aagcaagtct gtggggagcg
       61 gggcaggagt atctgtctgc gccgagactg gatgacgtgc agtgtgcgtt tgcttcctta
      121 acaggttttt tgcgggctgt tcctgcagac agcataccgg tgcttgcggt ttttgataac
      181 gaggaggtag ggtcatccac aaggcaggga gcttcttcca cctttttaaa ggacacgctg
      241 cttcggatca atgcggcact tggcagaaat atggaggatt atttttgtgc gatttccgaa
      301 agctttatga tatcggcaga taacgcacac gcatttcatc caaattatac ggataaagcg
      361 gatccggtaa atcatccggt aatgaacggc gggattgtga tcaaatacag tgccgatcag
      421 aaatatacaa cggacggtgt nnnnnnnnnn gcggcagtct gcaaaatatt atgtgaaaaa
      481 gcagacattc cttatcagat ctttgttaat cattccgatc tgccgggagg ttcaacgctt
      541 ggcagtcttt caggcaatca ggtggcagtc agtactgcgg acgttggaat tgcacagctc
      601 gccatgcatt ccccttatga gacatgcggt gtaaaagaca cggaatacat ggtgcggctt
      661 gcgcaggagc tgttttcttc ttccgtatgt caggacggaa acgaaagata tagaattata
      721 taaaagaaac gcacatatcc ttgataatac cggcattatc ctgtataatt ttagattgat
      781 aggccatgat ttctatatcg tttgagggga tgatatacct gtttaaaaga tcaggctgtc
      841 aatcacagat attaaggggt gcattggtaa atggatgatg aatggtatga agacgagggg
      901 gaagaacggg aacgtctgca cagaatacgc agggcggagc gtcttaagcg tatgcggcag
      961 gaaaaacgcc ggcagcagag gcttcgaagg tttttaggaa aaatagtgcc tcgtttgctt
     1021 gcaggaacag ccgttgtgct gttgcttgtt tttggtatcc gaaagatcgt ccggacagca
     1081 gacggcaatg agtcgggcca ggcggcagcg cagacagttt cgcaggaaac ggaggctgcg
     1141 acagttaaga tcgatgaaac tgtttccgat aacagtgcgg aagaaagcag ttctgatgca
     1201 ggggaagaaa ccggccagcc ggactactct gcggagaaaa cggaatatac actgcctttt
     1261 ggagatgata ttgtcagtac gcaggctatt tgaatagata cggaaacgaa tacgattctg
     1321 gcacaaagag atgcagacgc ggtgatcagt cctgcttcca tgacaaaaat tttaacgctt
     1381 cttgtggcgg ctgagcatgt aacagatctg gatgatacat ttacgatcac attggatatt
     1441 acggattaca gttttgaaaa tgactgcagt aatgttggct tttcggaaaa tgaaacggtt
     1501 accgtcagag acctttttta tgggacgatc cttccgtcag gtgctgacgc ggcagtcggg
     1561 cttgcaacgt atgttgccgg ttcacaggaa gcttttgtgg aactgatgaa tgaaaagctt
     1621 gatgagctgg gactttccga tacggcgcat gttacgaatt gtgttgggat ttatgatgag
     1681 gatcaccact gtacggtcta tgatatggca atgatattgg aagcagccat ggataatgag
     1741 ctatgcagaa cggttttgtc tgcacatact tatacaacgt ctgcaacgga agagcatccg
     1801 gaaggaatta ctatttccaa ctggtttctg cgccgcatcg aagataagga cagcgcggga
     1861 acagttattt gcggcaagac cggatacgtg gtgcagtccg ggaactgtgc tgccagttat
     1921 gcggaagatt cagatggaaa cggttatatc tgtgtgactg taaattcaac gagcagctgg
     1981 cgctgtattt atgatcacgt cgctatttat aagcagtttt tatcggctgc gggcggctga
     2041 gggaaattta aattaaatat attttcatat gattttagtt gaagatgttg tataataaaa
     2101 atgtgcttat gtaagaaata cggtagcagg aaggagataa attatgctcg caacattaat
     2161 tccgtttttt gataaaaata tgaaggtgtg cgcatattcg ttattttccc aaaaacagaa
     2221 tctgctgtta aatcccgtgc tgcttggctc cgggagaaat gacggggcgg cttcggtaaa
     2281 cgggcttgaa atcattcaaa atatggggat cgatacgctg tcaccggata cggatatttt
     2341 tgtaccggtt ggaaatattg cggttttttc cgatattgag tcacagtgca gtgtgcctca
     2401 tagccggctt gtcctgctgt tcgacaattc cgttatgaat gaagagattt atttgaagag
     2461 atttacccag ctgaaagaag aaggatataa gctggcgatc aggaagctgc cgatatccag
     2521 ttttgaagaa agccgtgagc tgatcagact gatggattat attattctgg actgtaaaaa
     2581 ggtcaatgtt atgaaggcaa agatctattt cggacatctg tttccgcata taaagatctg
     2641 cgtcggcaat attgaaaatc aggaaaagtt tgatgaagtg aaaaatgatg agagtttttg
     2701 gctgtttgaa ggtgaatttt atcgtattcc cgttacaaag ggccagacgg atgtagctcc
     2761 gctgaaagcg aattatctgg agcttttaaa gatggtaaat gatgcggatt tcgagcttac
     2821 gaaagcagcg gatgttattg gaagagatac ggctctcgta ttggagcttt tggaaatggt
     2881 caacagaatg agcgttaatt cagggattac gtcgatcaga catgcagccg caatgctcgg
     2941 acaaaaggaa ttgaaaaaat ggatcaatac cgcagtcaca aaagagcttt gcacggacaa
     3001 gccaagtgaa ataacgagaa tttcattgct tcgtgctaaa tttgcagagc agcttgcgcc
     3061 tatatttggt cttgctgcga aagattccga actgttcctg atggggctgt tttctgtttt
     3121 ggacttgata ttgaatcttc caatggagga tgcacttaag aagatcaatg tctcaaagga
     3181 tatttatcat gcacttgtat cacatgcagg agagtttgca aaggttcttg actttatgct
     3241 gcagtatgaa tacgcaaact ggcaggaagt ttccagacag atgatcatta tgaatattga
     3301 aatgtctgct gtgtatgatg catatatttc ttctatcaaa tggtac
//
LOCUS       DAOSYE010000141         3343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20484_length_3343_cov_3.160888, whole genome shotgun sequence.
ACCESSION   DAOSYE010000141 DAOSYE010000000
VERSION     DAOSYE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20484_length_3343_cov_3.160888"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLU43_03395"
     CDS             complement(<1..1153)
                     /locus_tag="PLU43_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOO27485.1"
                     /translation="MTAEKSSRKKNKPRFFFDYTLLFIVIFLLCFGLVMLYSVSSYEA
                     NIKFQDSAYYLKKQAQATIIGIVVMFITMMIDYHIWERFATIAYGVSAVLILLVLTPL
                     GYEANGARRWLNLGMSLQPAEVAKVGMIIFFASLVCKMGKRIQTKKGLLFTVAWALPI
                     AGMVFIITENLSSAVIIVGIVFMMLFVATPNYKEYFLAVAGVLLVAGLIVLAVEKGII
                     PEDMSYRFARIKAWLNPEAYASTKGFQTLQALYAIGSGDIFGKGLGQSMQKLGFLPEA
                     QNDMVFSIICEELGLFGAVAIMMLFLLMIWRFMVIADNAQDLFGALLVVGVMAHISIQ
                     VILNIAVVTNTVPNTGITLPFISYGGSSVVFLLIEIGLVLNVSKSIRFEK"
     gene            complement(1150..2493)
                     /gene="murD"
                     /locus_tag="PLU43_03400"
     CDS             complement(1150..2493)
                     /gene="murD"
                     /locus_tag="PLU43_03400"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOO27486.1"
                     /translation="MGKILVVGSGLSGLSAVRLLIQVQKTPVLFDSNEKLDPEEIRKK
                     LQIKKEVEIYTGNLPDDVAAQIEMLVLSPGVPIDCEFVNRFRHAGVKISGEIELAYTY
                     DRGRLLAVTGTNGKTTTTALLGAVMQDYFKQVFIVGNIGKPYTEAVLNSTKGSVTVAE
                     ISSFQLETIRYFHPCVSAILNISPDHLNRHHTLQQYILEKEKIAVNQTKDDTVVLNYE
                     DDVTRKFGNITTARPLYFSSKRVLEDGFYLKDGMLCMKRDEKEQELLAVGDLQIIGMH
                     NYENAMAAIAMAFSFGVPMESIVASVKRFKAVAHRIEFVAEKDGVVYYNDSKGTNVDA
                     AIKGIRAMTRTTCLIGGGYDKGAAYDEWIEAFDGKVKKLVLLGATAKMIAESAQSHGF
                     TDYVFAKDLEEAVMICKDTAESGEAVLLSPACASWGMFDNYEQRGDMFKELVRKL"
     gene            complement(2519..>3343)
                     /gene="mraY"
                     /locus_tag="PLU43_03405"
     CDS             complement(2519..>3343)
                     /gene="mraY"
                     /locus_tag="PLU43_03405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOO27487.1"
                     /translation="LKKNGTPTMGGIIILGSMFLTSLFFIGKYPGVLPVLLLTAGFGL
                     IGFIDDYIKVILKRSMGLRAWQKMLLQIFVTGVFAFYMANISGIGLTMRIPFSNGSYW
                     DAGWLAVPILFLAVLGTVNGANFTDGLDGLASGVTVLIAAFFSILSIMNQGGIEPATL
                     AMTGALLGFLLFNVYPAKVFMGDTGSLALGGFVIASAYMLQMPLYILLVAFIYLLEVL
                     SVILQVGYFKLSGGKRIFKMAPIHHHFELCGWSETRVVAVFTIVTTILCVIGFMAY"
BASE COUNT          987 a          905 c          590 g          861 t
ORIGIN      
        1 atttctcaaa tcggatgctt tttgaaacat tcagaaccag tccgatctca atcagaagga
       61 acacgaccga agatccccca tagctgataa aaggcaaagt aatacctgta tttggcactg
      121 tgtttgtcac aactgcaata ttcagtatta cctgtatgga aatatgtgcc ataacaccaa
      181 caactaacaa tgcgccgaac agatcctgcg cgttgtcggc aataaccatg aacctccaaa
      241 tcatcaacag aaacagcatc atgatcgcca ctgctccaaa cagccccagc tcttcacaaa
      301 tgatggaaaa taccatgtca ttctgcgctt ccggaaggaa cccgagcttc tgcatgcttt
      361 gaccaagtcc tttcccgaaa atatcaccgg agccgatcgc ataaagcgcc tgcaatgtct
      421 gaaacccttt tgtgcttgca tatgcttccg gattcagcca cgctttgata cgcgcaaaac
      481 gataggacat atcctcagga ataatgcctt tttcgaccgc aagcacgatc agaccggcta
      541 caagaagaac accggccact gcaagaaaat actctttata atttggtgtt gccacaaaaa
      601 gcatcataaa tacgatgccg actataatga ccgcagagct taaattttcc gtaatgataa
      661 acaccatgcc ggcgatcggc agcgcccagg caaccgtaaa cagcagtcct ttttttgtct
      721 ggatccgttt tcccatttta cagacaagac ttgcgaaaaa aatgatcata ccgacctttg
      781 caacctcagc cggctgcagg gacatcccaa gattcagcca tcttctggca ccatttgctt
      841 cataaccaag cggtgtcagt acaagcagaa tcagcacggc cgatactccg taggcaatcg
      901 ttgcaaacct ctcccatata tgataatcga tcatcattgt aataaacatg acaacaatgc
      961 cgataatcgt tgcctgtgcc tgttttttta aataatatgc ggaatcctga aacttgatgt
     1021 tggcttcata ggaactgaca ctgtagagca taacaagtcc gaagcacaac aaaaaaatga
     1081 cgataaacaa caatgtatag tcaaagaaga aacgaggttt attctttttt ctgctgcttt
     1141 tttctgctgt catagttttc taacaagctc cttaaacata tccccgcgct gttcataatt
     1201 atcaaacatg ccccagctcg cgcacgccgg agacaacagt accgcttctc ccgattctgc
     1261 tgtgtctttg cagatcataa ccgcctcttc cagatctttt gcaaaaacat aatcggtaaa
     1321 tccgtgcgac tgagcacttt cagcaatcat tttcgccgtt gcaccgagca gcaccagttt
     1381 ttttactttt ccgtcaaatg cctctatcca ttcatcatat gccgcgccct tatcataacc
     1441 gccgccgatc agacaggtcg ttcttgtcat tgcacgaatc cccttgatcg ccgcatccac
     1501 atttgtacct ttggaatcat tataataaac aacgccgtct ttttcagcaa caaattcgat
     1561 ccgatgggct accgccttaa aacgctttac ggatgcaacg atactttcca ttggtacccc
     1621 aaaggaaaac gccatggcaa tggcagccat tgcattttca taattatgca taccaatgat
     1681 ctgcagatcg ccaacagcca gaagttcctg ttccttctca tctctcttca tgcagagcat
     1741 accgtctttt aaataaaatc cgtcctccaa aactcttttg gagctgaaat ataacggccg
     1801 cgcagtagtg atattcccaa atttccgcgt tacgtcatct tcataattca aaacaaccgt
     1861 atcgtccttt gtctgattca ctgcaatttt ttctttttcc agaatatatt gctgcagtgt
     1921 atggtgtctg ttcagatgat caggagaaat attcagtatc gcgctgacac atggatgaaa
     1981 atatctgatc gtttccagct gaaagctgct gatctccgca actgtcaccg atcctttcgt
     2041 ggaattcagc actgcctccg tatagggctt cccgatattg ccgacaataa acacctgctt
     2101 gaaataatcc tgcataacag cgccaagcaa tgccgttgta gtcgtctttc cattcgtacc
     2161 tgtcacagcc agcagacgcc ctctatcata cgtatatgcc agttcgatct ctcctgatat
     2221 ttttacacct gcatgacgga atctatttac aaattcgcag tctattggaa cacccggact
     2281 taaaacaagc atttcgatct gggccgccac atcgtccggc aggttccctg tatagatctc
     2341 aacctctttt ttgatctgca gttttttccg gatctcttcc ggatccagtt tctcattaga
     2401 atcaaaaagc accggtgttt tttgcacctg tatcaaaagt ctgacagcac tcagtccgct
     2461 taatccgctg ccgaccacaa gaatttttcc catatccgct tcctcttctt accattccct
     2521 aatatgccat gaacccgatc acacataaaa tcgtggtcac gatcgtaaaa acagcgacaa
     2581 cccttgtttc ggaccagccg caaagctcaa aatgatgatg gatcggcgcc atcttgaaaa
     2641 tacgctttcc tcctgacagt ttaaaatacc cgacctgcag aataacagaa agtacttcca
     2701 aaagataaat gaacgccaca agtaaaatat ataaaggcat ctgaagcata taggcactcg
     2761 caatgacaaa cccgccaagc gcaagagaac ccgtatctcc cataaaaacc tttgccggat
     2821 acacattaaa caacaaaaat cccagcaatg cacccgtcat ggcaagtgtg gccggttcaa
     2881 tgcctccctg attcataatc gacaatatgg aaaaaaacgc tgcaatcaaa acagtgaccc
     2941 cggacgcaag accgtccagc ccgtctgtaa aatttgctcc attcacggtt ccaagcaccg
     3001 ccagaaacaa gatcggaacc gcaagccagc cggcatccca gtaactcccg tttgaaaagg
     3061 gaattctcat tgtcagtccg atcccggata tatttgccat ataaaatgca aatacacctg
     3121 ttacaaaaat ctgcagcagc atcttctgcc atgcacgcag acccatcgaa cgcttcagaa
     3181 taaccttgat ataatcatca ataaagccga tcagtccgaa tcctgccgtc agcagcagca
     3241 ccggaagtac acccggatat tttccaataa aaaacaatga cgtaagaaac atactgccaa
     3301 gtataataat accgcccatc gtaggcgttc cgtttttctt cag
//
LOCUS       DAOSYE010000142         3342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20490_length_3342_cov_3.011865, whole genome shotgun sequence.
ACCESSION   DAOSYE010000142 DAOSYE010000000
VERSION     DAOSYE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_3342_cov_3.011865"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLU43_03410"
     CDS             complement(<1..1177)
                     /locus_tag="PLU43_03410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27488.1"
                     /translation="MHKFSEDLFQNAIDLNETIVFEYDIREDVISFSDNMKKYIPMPF
                     YVSTFVEKIDVHGKIHQDDIRKAITFFSSASDMGKVRMEYLRFLDFYGDFRWYQLKAR
                     LEKGEDGRPVLLYGTMTYVDDETKQHYDEALYSKDELTHLLTKESLFQSIDEYIANIA
                     KDVIPDMLIVDIDDFIEWKETHGNISAEGMIVEVSRILKRAFRGSDLIGRISEDRFAI
                     FMKGVHAVSILLERAAYVRQTVKEVWSDFENNAGLTVSIGIAAMHAMEATADKLYARA
                     LAALDDAKRSGKDTYVLYTGEMERLDTTVNPILSTKEMELIRNVLDPMCAWAYAVDEN
                     YQILYRNEMLEKRLNNASSGLCYVQNKGYSEPCPDCPLRLMDDKKASLDSVVYSTSMR
                     "
     gene            complement(1272..2435)
                     /gene="hemW"
                     /locus_tag="PLU43_03415"
     CDS             complement(1272..2435)
                     /gene="hemW"
                     /locus_tag="PLU43_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOO27489.1"
                     /translation="MKKPLGLYVHIPFCVQKCRYCDFLSFSSDRETQERYMDAVRREI
                     DMIPEKYADTDFSGYCVQTVYFGGGTPSILSPDLLGSILCKLNTSFFISENAEITMEA
                     NPGTLSKEILPAYREFGINRLSIGLQSADDEELKLLGRIHTWRDFTDNYEAAKRAGFQ
                     NINVDIMTALPGQAEATLDKTIEKVLLLEPAHISAYSLIIEEGTLFHEWFKEDGKGEP
                     VQSLLLPDEEQERRMYWHTGDMLREAGYVHYEISNFAKPGFESRHNLSYWTGIDYLGL
                     GLGASSLLSHVRYKNPAELDIYIREPGRQGERLVLEETAQMEEFMFLGLRMQRGVGKR
                     EFFSLFHVPVTQVYGKEIIKLKREGLLYEEMGRIALTKRGIDYGNYVFSAFLK"
     gene            complement(2444..3342)
                     /locus_tag="PLU43_03420"
                     /pseudo
     CDS             complement(2444..3342)
                     /locus_tag="PLU43_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281246.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
BASE COUNT          868 a          911 c          609 g          954 t
ORIGIN      
        1 tacgcatgga agtcgaatag acaacggaat ccagcgatgc ctttttatca tccatcagac
       61 gcagcggaca gtcaggacac ggctcactgt agcctttatt ctgaacataa caaagaccgc
      121 tgcttgcatt gttcagtctt ttttcaagca tctcgtttct gtaaaggatc tgataatttt
      181 catccaccgc atacgcccat gcacacatcg gatccagaac atttcggatc agctccattt
      241 ctttcgtgga aaggatcgga ttcacagtcg tatccagccg ctccatttct cccgtataca
      301 gcacataggt atccttgccg gatctctttg catcatcaag cgcagccagg gcacgggcat
      361 aaagtttatc agccgtggct tccatggcat gcatcgccgc aatgccaata ctgacggtca
      421 atcccgcatt attttcaaaa tcactccaga cttccttcac tgtctgccgg acataagccg
      481 ccctttctaa caggatgctc accgcatgaa cgcctttcat aaaaatcgca aaccggtcct
      541 cactgatcct gccgatcagg tcggagccgc ggaaagcacg ctttaagatc cttgaaacct
      601 caacgatcat cccctctgcg ctgatattcc catgagtttc tttccattcg ataaaatcgt
      661 caatatctac gatcagcatg tccgggatga catctttcgc aatattcgct atatattcgt
      721 caatactctg aaacagcgat tcctttgtca gaagatgggt cagttcatct ttggaataca
      781 gtgcctcatc ataatgctgc ttcgtctcgt cgtcgacata ggtcatcgtt ccgtacagca
      841 gcaccggccg gccgtcttct cccttttcca gccttgcctt cagctgatac cagcgaaaat
      901 caccgtaaaa atcaaggaaa cgaagatatt ccattcgaac ctttcccata tcacttgccg
      961 aagaaaaaaa agtgatcgct tttctgatat catcctgatg tatctttcca tgtacatcga
     1021 tcttttcgac aaacgtagaa acataaaacg gcatcggaat atattttttc atattgtcgg
     1081 aaaaactaat gacatcctca cgtatatcgt actcaaatac gatcgtctcg ttcagatcaa
     1141 ttgcattctg aaacaaatct tcactgaatt tatgcatcaa atacatccct ttcccatatc
     1201 tgctatgtaa tgttttgcca ctggttatag tatacaaaat tctcatgaaa aggacaatgt
     1261 ttttagttat tttatttcaa aaatgcggaa aatacataat tcccatagtc aatacccctt
     1321 tttgtaagtg caattctgcc catttcttca taaagaagac cctcccgctt aagttttata
     1381 atctctttcc catatacctg cgtcaccggc acatgaaata acgaaaaaaa ctcccgtttg
     1441 ccgacccctc tttgcatccg cagaccaaga aacataaact cttccatctg tgcggtttct
     1501 tccaaaacca gccgctcccc ctgcctgccg ggttccctga tatagatgtc cagctccgcc
     1561 ggatttttat aacgtacatg cgaaagcagg gacgaagcgc caagaccaag cccgagataa
     1621 tcgattcctg tccagtaaga cagattatgc cggctctcaa agcccggctt tgcaaaattg
     1681 gaaatctcat agtgaacata accggcttct cgcagcatat ctcccgtatg ccagtacata
     1741 cgcctctcct gttcttcatc cggaagcaaa aggctctgca caggctcacc ttttccatcc
     1801 tctttgaacc actcatggaa cagtgttcct tcctcgataa tcaggctgta cgctgaaata
     1861 tgcgccggct ccagcaaaag caccttttca atcgtcttat caagtgtggc ttccgcctga
     1921 cccggcagag ccgtcatgat atctacattt atattttgga agcccgccct tttggcagct
     1981 tcataattat ctgtgaaatc tctccatgta tgaatgcgtc caagaagctt cagctcttcg
     2041 tcatccgcag actggagtcc gatacttaag cggttgatgc cgaattcgcg ataagccggc
     2101 agaatttctt tcgacagcgt tcccggattt gcctccatcg taatttccgc attttcagat
     2161 atgaaaaaag aagtgttcag tttacataat attgatccaa gtaagtcagg agacagaata
     2221 ctcggtgttc cgccgccgaa ataaacagtt tgcacacaat acccggaaaa gtccgtatcg
     2281 gcatatttct ccggtatcat atcgatctcc ctgcgcacag catccatata cctctcctgc
     2341 gtttccctgt cggatgaaaa cgacaggaaa tcgcaatatc tgcatttttg tacgcaaaac
     2401 ggaatatgca cataaagtcc aagcggcttt ttcatctgct tccctacttc ctgtcgtcca
     2461 gcttcagtac gctcataaat gccttctgcg gaatttcaac gtttccgatc tggcgcattc
     2521 ttttcttgcc ttccttttgt ttctcaagaa gctttctttt tctggtgata tcgccgccat
     2581 agcattttgc aagtacatcc ttgcgcatcg ccttaacagt ctccctcgct atgatcttgc
     2641 tcccgattgc tgcctggatc ggtatttcga ataagtgtct tggaatttct tccttcagtt
     2701 tttcgcacat tttccttccg cgctcataag cactgtccgc atgtacgata aaggaaagcg
     2761 cgtcgacttc ttccctgttg atcagaatat ccagttttac aagctcggaa cgttcaaccc
     2821 ttcatatcat agtcaaatga cgcataccct ttcgaacggg actttaatgc atcaaaaaag
     2881 tcataaataa tttcattgag cggcagctca tattttagga gcgcacgggt ctcttccata
     2941 tattccatgc caagatacct gcccctgcgt tcctgacaca gttccatgat cgagcccaga
     3001 tatgcggtcg tcaccatgat ttctgcgctg acgatcggct cctccatata atcgatttca
     3061 gacggatccg gcagattaga cggattcgtc agttctatta catctccatt tgttttatat
     3121 actttataaa taacaccggg cgccgttgtc acaaggtcca gattgtattc cctctccagc
     3181 cgttcctgaa tcacctcaag atgaagcagc cccagaaagc cgcagcgaaa tccaaatcca
     3241 agcgcgatcg atgtctccgg ttcataattc agcgatgcat catttaactg cagtttttca
     3301 agtgcatcac gcagatccgg atatttcgcc ccatccgcag ga
//
LOCUS       DAOSYE010000143         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20541_length_3337_cov_2.916210, whole genome shotgun sequence.
ACCESSION   DAOSYE010000143 DAOSYE010000000
VERSION     DAOSYE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20541_length_3337_cov_2.916210"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLU43_03425"
     CDS             complement(<1..236)
                     /locus_tag="PLU43_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HOO27490.1"
                     /translation="MIKLHEGGAYLIDGAELIPDTEDALAQVAEAVGRDVSQEEAEKN
                     TIAYGILKAHNTSDNMEKLKIKFDKLTSHDITFVG"
     gene            398..1288
                     /locus_tag="PLU43_03430"
     CDS             398..1288
                     /locus_tag="PLU43_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27491.1"
                     /translation="MIDRIIPEKSDNMLAIADPAAENISYGGNQAWFTGNTQAYAGCG
                     PVAAVNMLLCLMQKYPEPIKELLAKAGLSASSSNSFCPPFLKDDILHLMNEIYLTMGT
                     LEIPVLNRLYDRSDRKNPLFKVLSPNHGRSIPGFCRGILKYAGNHGIFLHADVLPTAF
                     CPYETGLRFIKEGLEKSGSVVLLTSFNRHPLTLFKETSEGTMVPYEHKGGMCCHFAVI
                     TDIMAETAPVLKLSTWGKAAAVEYRLLAASWQSRRAFDSALFYFTPAKTSDITAQDMK
                     QAYRVLLQALWQTLFKKIRT"
     gene            1358..2227
                     /locus_tag="PLU43_03435"
     CDS             1358..2227
                     /locus_tag="PLU43_03435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO27492.1"
                     /translation="MTETSAVLIILNYNDKNTTQTLVNAIKGYTCLHKIIVVDNSSSD
                     GSFAYLKKTLTSDKVDLIQAPENGGYAKGNNFGIRYAIKTYAPDYLFIANPDIMVKEP
                     VLSVILKTMEQQPDYGIMSCLVNQGYNVWNLPGFAGMIESLFLLWFNLDKYMIRKKLL
                     SSANALEPVGVIEGSFFCIKKTAYEAIGGLDERTFLYAEEIILSRRLSKKDLKAGILT
                     KERFDHFHSVSIKKAYRSSKAKAFHNFYNSFRIYNKEYLHTSPLQDGIFAAAYKLAYI
                     ERIIYDLVMRIKR"
     gene            complement(2235..>3337)
                     /locus_tag="PLU43_03440"
     CDS             complement(2235..>3337)
                     /locus_tag="PLU43_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO27493.1"
                     /translation="VYPYKGGISHYTGLLYRTLSEKYNTEMISYKMQYPKLLFKREQK
                     DFSNDTFRVDDAKFLLNTANPFNILSVGRRIRKERPALVIMEWWHPYFAPCYYILSKM
                     LKGIKLLFICHNVFPHERFPLDKKLTRLVLKQGSCFILHSGTEADDLLTIKGDAEYSV
                     QVHPTYAAFQFRKMGKEEARKQLCVSEERQLLLFFGYVREYKGLKHLIDAMGILAGEG
                     GGKQLPELYIVGDFDGNRESCMEQIQACGVENLVTIVDGYVPDREVEKYFAASDLVVL
                     PYESATQSGIVQIAYGFHKPVIVTNVGGLPEVVEDDRTGMIVEPCDARQLADAISRFF
                     EEHKAEEYEKNIRENEYRFSWERMREAIEGFL"
BASE COUNT          912 a          824 c          670 g          931 t
ORIGIN      
        1 ccgacaaacg taatatcatg ggatgtcagc ttatcaaact ttattttcag tttttccata
       61 ttatcggatg tattatgtgc ctttaagatt ccataggcaa ttgtattttt ttcagcttct
      121 tcctgcgaaa catccctgcc gacagcttct gcgacctgtg caagtgcatc ctcagtatcc
      181 ggaatcaatt ccgcaccatc tatcagatag gcgccgcctt catgcaattt aatcattcca
      241 aatcctcctc tgttatcctc tttcaatcgt taaacaatat caatactcat tttatagtag
      301 tgggctgctt ttcgcaaggg gtttgtggta tacttcaata gattgaacga aatctttaga
      361 ataaaacata aaacaatatg atcggaacaa gataactatg attgatcgaa tcatccctga
      421 aaaatcagac aatatgctgg ccattgcgga tcctgctgcc gaaaatatct cctatggcgg
      481 aaatcaggca tggtttacgg gcaataccca ggcttatgca gggtgcggcc cggtagccgc
      541 tgtgaatatg ctcctgtgcc taatgcaaaa atatccggaa ccgataaaag aactgcttgc
      601 aaaggcaggt ctgtcggcat cttcttccaa ttctttctgc ccgccttttt taaaagatga
      661 cattttacat ttgatgaacg aaatttatct cacaatgggc acactggaaa ttcctgttct
      721 gaacaggctc tacgatcggt ccgatcggaa aaatccgctt tttaaggtac tgtctcccaa
      781 tcatggaaga agtatccccg gcttttgcag gggaatttta aaatatgcgg ggaatcatgg
      841 aatctttctc catgcagatg tgctgccgac cgcattctgc ccctatgaga ccggactgcg
      901 ctttattaag gaaggccttg aaaaaagcgg ttctgttgta ttactgacct cctttaaccg
      961 tcatccgctg acacttttta aggagacctc agagggcaca atggttccat acgagcacaa
     1021 gggcggcatg tgctgccatt ttgcagtaat tacggacatt atggcagaaa cggctcctgt
     1081 gctgaagctt tctacctggg ggaaggccgc agctgtcgaa tatcggctgc ttgcagcttc
     1141 ctggcaaagc cgcagggcct ttgacagtgc tttattttat tttacgcctg caaaaacttc
     1201 cgatattacg gcacaggata tgaaacaggc atatcgggtg ctgctgcagg ctttatggca
     1261 gacacttttt aaaaaaatcc gaacataaca gccgttgtcc ggactttcta tttatgataa
     1321 gataaataac agtaacaaac agaaaggaac tatggccatg acggaaactt cggctgtact
     1381 gatcatcctg aactataacg ataaaaatac gacacagaca ctggtcaatg ccataaaggg
     1441 atatacgtgc cttcataaaa tcattgttgt tgacaacagt tcctccgacg gctcctttgc
     1501 ttacttaaaa aaaacactga cttccgacaa ggtagatctt atacaggctc ccgagaacgg
     1561 cggctatgca aaggggaata atttcggcat tcgatatgcc ataaaaacct atgctcccga
     1621 ttatctgttt atcgccaatc ctgatattat ggtcaaagag ccggtcttgt ctgtcattct
     1681 aaagaccatg gaacagcagc cggattacgg tattatgagc tgtctcgtca atcagggcta
     1741 taatgtctgg aatcttcccg gttttgccgg catgatcgaa agcctctttt tactgtggtt
     1801 caatctggat aaatatatga tcagaaaaaa actgctctct tccgcaaacg cccttgaacc
     1861 tgtcggcgtg atcgagggct cgtttttctg cataaaaaaa accgcgtatg aagcaatcgg
     1921 cggtcttgac gaacgcacct ttctctacgc ggaagagatc atcctgtcca gacgtttgtc
     1981 taaaaaggat ctgaaagccg gtattttaac aaaagaacgc tttgatcatt ttcattcggt
     2041 cagcatcaaa aaagcctacc gttcctcaaa agcaaaagca tttcataact tttacaacag
     2101 ttttcgaatc tataacaaag aatatctgca cacaagtccc ctgcaggacg gcatttttgc
     2161 cgctgcatat aagcttgcct acatagagcg catcatttat gatcttgtga tgcgcataaa
     2221 acgctgagcc ggccttacaa aaagccttct attgcctccc gcatccgttc ccaggaaaaa
     2281 cggtattcat tctcgcggat atttttttcg tattcctctg ccttatgctc ctcaaaaaaa
     2341 cggctgatcg cgtctgcaag ctgtctcgcg tcacacggct ccacgatcat tcccgtccgg
     2401 tcatcctcga cgacctccgg cagtcctccg acatttgtta caatgaccgg cttatgaaat
     2461 ccgtaggcaa tctgtacgat tccgctctgt gttgcagact cataaggaag cacgaccaga
     2521 tcacttgcgg caaaatattt ttccacttcc cggtcaggca catacccgtc tacgatcgtc
     2581 accaggtttt ccactccgca ggcttggatc tgttccatgc agctttccct gttcccgtca
     2641 aaatcaccga caatataaag ctcaggcagc tgttttcctc cgccttcccc tgcaagaatt
     2701 cccatggcat cgatcagatg ctttaatccc ttatattcac gcacatatcc gaaaaacagc
     2761 agcagctgcc tttcctctga aacgcaaagc tgctttctcg cttcttcttt tcccattttc
     2821 cgaaactgga atgccgcata agtcggatgc acctgtacgc tgtactctgc atcgcccttt
     2881 attgtcagaa gatcatcagc ctccgtaccg gaatgcaaaa taaaacagct gccctgcttc
     2941 aatacaagcc ttgtcagctt tttatccaaa gggaagcgtt cgtgcggaaa tacattatga
     3001 cagataaaca gcagtttgat ccctttcagc atttttgaaa gaatataata gcagggcgca
     3061 aaatacgggt gccaccattc cataattaca agtgccggac gctcctttct gatcctgcgg
     3121 cctaccgaca gaatattgaa tggatttgcc gtattgagca gaaactttgc atcgtccacc
     3181 cgaaaggtat cattggaaaa atccttctgt tcccgcttaa aaaggagttt cggatactgc
     3241 atcttatagg agatcatttc tgtattgtat ttttccgaaa gtgtccgata caaaagaccc
     3301 gtatagtgag agatcccgcc tttatacgga tataccg
//
LOCUS       DAOSYE010000144         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20570_length_3334_cov_3.298872, whole genome shotgun sequence.
ACCESSION   DAOSYE010000144 DAOSYE010000000
VERSION     DAOSYE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20570_length_3334_cov_3.298872"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLU43_03445"
     CDS             <1..623
                     /locus_tag="PLU43_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOO27494.1"
                     /translation="PVVYLTDCYWRRENYGEIKELYESGRTREFILIGIGYPDNYDFD
                     VIRVRDLINDPDSFLSLIVNGIMPYAEGTYSVDASDRTFCGASYGGNFMLYSLFQSDG
                     ITKGLFKNYVMASPIFYKRSYEQYLFDYEEAYYKRGNTSVNANVYMTVGEDENILLVR
                     KFKKFTARLERRNYTGLNLTAKVYEGKEHYTVWVPTLLEGLKMFLE"
     gene            771..1484
                     /locus_tag="PLU43_03450"
     CDS             771..1484
                     /locus_tag="PLU43_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOO27495.1"
                     /translation="MSSSKHKAWRALKAAFPYTIPIFAGFWFLGLTYGIYMHVSGFSF
                     LYPMFMSLLIFGGSLEFVVVNLLLGAFDPLQAFTIALMVQARHLFYGISMLDRFKGTG
                     WKKPYLIFGMCDESFSIIYTAKIPEDVDKGWFMFWVTALNHFYWFSGSTLGGLLGSLL
                     KFNTTGLEFVMTAMFTVIFLEQWMKEKKHYTALLGLGCSLLCLILFGASDFMIPTMVS
                     LLGVLTVLRRPIEKAGGFV"
     gene            1493..1819
                     /locus_tag="PLU43_03455"
     CDS             1493..1819
                     /locus_tag="PLU43_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HOO27496.1"
                     /translation="MTILQQVIIIAVCILATMLTRFLPFFIFSGKKPAPHYIQYLGKA
                     LPAAVFGMLVIYCLKDVSIFTGMHALPELISIAFVIALHLWKRQMLLSIAGGTVCYML
                     LIQFVF"
     gene            1834..2565
                     /locus_tag="PLU43_03460"
     CDS             1834..2565
                     /locus_tag="PLU43_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27497.1"
                     /translation="MNAIEINGLKKKYSDFTLEDISFSLPEGSILGLVGENGAGKSTT
                     INLIMNAVSRDGGTVTVLGTDNRSEEFKKTKEDIGIVLDEAYFPPVISPVNVNSIMKY
                     TYQNWDEHKFFGYLKKFALPEKKKFKDFSRGMKMKLAIAAALSHDPRLLILDEATSGL
                     DPIIRDEILDIFKEFAAKENHSILLSSHIVSDLEKLCDYIAFIHAGKLVFCEKKEVVL
                     DKYALSEHTTLEDIIVFLAKGERLG"
     gene            2562..3185
                     /locus_tag="PLU43_03465"
     CDS             2562..3185
                     /locus_tag="PLU43_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOO27498.1"
                     /translation="MKALLIKDFYTLVKQMKMFIVLILIFAILPGTSLTAFAVIYCAM
                     LPMTAISYDENCKWDKLAVMMPYSAKSLVFEKYVFGYALIAASAVISLLSEAVLSVIR
                     GTEFGIGSLGMIAAVICVALVMQAINLPFIYKFGVEKGRFVYLVVFVAAFALIGVAGK
                     GNDLSGLVSIVHWNPVFLACALLVVTALLNVGSILLSERFYLKKNRN"
     gene            complement(3187..>3334)
                     /locus_tag="PLU43_03470"
     CDS             complement(3187..>3334)
                     /locus_tag="PLU43_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOO27499.1"
                     /translation="PEEVHAIIAEIPADRMGTAEEAAALVLQLCTGNTYLTGQIINLD
                     GGWI"
BASE COUNT          878 a          585 c          831 g         1040 t
ORIGIN      
        1 atccggttgt ttatttgacg gactgctatt ggagaagaga aaattacggt gagatcaaag
       61 aactgtatga gtccggaagg acaagggagt ttattctgat cgggatcggc tatccggata
      121 attatgattt tgatgtgatc agagtcaggg atctgatcaa tgacccggac agctttttat
      181 cactgatcgt aaatggtatt atgccgtatg cagagggaac ctattctgtt gacgcttcgg
      241 accgtacgtt ttgcggcgcc tcctatggcg gcaattttat gctgtacagt ctgtttcaga
      301 gcgacggcat tacaaagggc ttatttaaaa attatgtaat ggcaagtccg atattttata
      361 agcgaagcta tgagcagtat ctttttgatt atgaagaggc atattataaa agggggaata
      421 cttccgtaaa tgcaaacgtc tatatgactg tcggagaaga tgaaaacata ttacttgtca
      481 gaaaatttaa aaaattcacg gcacggcttg aaagaaggaa ttatacaggc ctgaatctga
      541 cagcaaaggt ttatgaagga aaggagcatt atacggtctg ggtgccgacg ctgctggaag
      601 gtctgaaaat gtttttggaa taatgtgaac attcccgtat aggatgcaga gttgtttttc
      661 tgtacaaaat gagtgcgtgt gtggtatact tttatgcagc agcaatggac attccgataa
      721 aagaatgtct attgttgctt tatataggaa cagaaagagg tactgatgat atgagcagca
      781 gtaaacataa ggcgtggcgt gcactgaagg cggcatttcc ctatacgata ccgatattcg
      841 ccggtttttg gtttttagga ctgacatacg gaatctatat gcacgtatcg gggttcagtt
      901 ttttatatcc gatgtttatg agtttgttga tttttggcgg ttccctggag tttgttgttg
      961 tgaatctgct tttgggagcc tttgatccgc ttcaggcatt tacgatcgcg ctgatggtac
     1021 aggcacggca cctgttttac ggaatttcca tgctggacag atttaaggga accggctgga
     1081 aaaaaccata tttgatcttt ggcatgtgtg acgaaagctt ttctattatt tatacggcaa
     1141 aaatcccgga agatgttgat aaagggtggt ttatgttctg ggtgacggca ctgaatcatt
     1201 tttactggtt ttccggttct acactcggcg gtctgcttgg ttcgcttttg aaattcaata
     1261 caacagggct tgagtttgtt atgacggcaa tgtttaccgt gatttttctg gaacagtgga
     1321 tgaaggaaaa aaagcattat acggcactgc tcggactggg ctgttctctt ttatgcctga
     1381 ttttattcgg ggcatcggac tttatgattc cgacgatggt ttccttgctc ggagttctga
     1441 ctgttttgcg cagaccgatt gagaaagcgg gagggtttgt ataatggagt ccatgacgat
     1501 tttacagcag gtgattatca ttgcggtctg tattttagca acgatgctga caagatttct
     1561 gccatttttc attttttcag ggaaaaagcc cgcgccgcat tatattcagt atctcggaaa
     1621 agcgcttccg gctgctgttt ttggaatgct tgttatttat tgtcttaagg atgtcagtat
     1681 ttttacgggc atgcacgcgc ttccggaatt gatctccata gcgtttgtga tcgcactgca
     1741 tctatggaaa cggcagatgc tgctttccat agcaggcgga accgtgtgtt atatgctgct
     1801 gatccagttt gtgttttaat aataggaggt tctatgaatg caatagaaat aaacggatta
     1861 aaaaagaaat attcggattt tacacttgaa gatattagtt tttccctgcc ggaagggagt
     1921 attctgggtc tggtcggaga aaacggggcg ggcaagagta caacaatcaa tctgatcatg
     1981 aatgcggtct cccgtgacgg cggaaccgta acagttcttg gaacggataa ccggtctgag
     2041 gaatttaaga agacaaaaga ggacattggc attgtactgg atgaagctta ttttccaccg
     2101 gtcatttctc ctgtgaatgt gaattccatt atgaaatata cgtatcaaaa ctgggacgaa
     2161 cataaatttt ttggttattt aaagaagttt gcgcttccgg aaaaaaagaa attcaaggat
     2221 ttttcacggg gcatgaagat gaagctggcc attgcggccg cgctgtctca tgatccaaga
     2281 cttctgatct tagatgaagc gacaagcgga ctcgacccga tcatccgtga tgagatactg
     2341 gatattttta aagagtttgc agcaaaggag aatcattcga tacttctttc ttcgcatatt
     2401 gtcagtgatc tggaaaagct ttgtgattac attgccttta ttcatgccgg gaaactggta
     2461 ttctgcgaaa aaaaggaagt ggtgcttgat aaatatgcgc tgtctgagca tacaacgtta
     2521 gaagatatta ttgtgttttt ggcaaagggg gaacgcttag gatgaaagca ttactgataa
     2581 aagattttta tacactggtc aagcaaatga aaatgttcat tgttttgatc ctgatctttg
     2641 ccatactgcc cggaacatcc ctgaccgcat ttgcagttat ttattgcgcg atgcttccaa
     2701 tgacggcaat ttcttatgat gaaaactgta aatgggataa actggctgtc atgatgcctt
     2761 attccgcaaa aagtcttgta tttgaaaagt atgtgttcgg gtatgccctg atagctgctt
     2821 cagcagttat ttccctctta tccgaagcag tgctttcagt gatacggggc acggaatttg
     2881 ggatcgggag tcttggtatg atcgcagctg ttatttgtgt ggcgctggtc atgcaggcga
     2941 tcaaccttcc gtttatttat aaatttgggg tagaaaaggg acgatttgta tatctggtcg
     3001 tgtttgttgc ggcttttgca ttgatcggag ttgcaggaaa agggaatgat ctgagtggac
     3061 ttgtttctat agtacattgg aatcctgttt ttctggcatg tgcactgctt gttgtaacgg
     3121 cattgttaaa tgtcggctcg atacttcttt ccgagcggtt ctatctaaag aaaaacagga
     3181 attaatttag atccatccac cgtccagatt gatgatctgt ccggtcagat aagtatttcc
     3241 ggtacaaagc tgcagtacaa gagcggccgc ttcttctgcc gtacccattc tgtccgcagg
     3301 aatttccgca atgatcgcat ggacttcttc cggt
//
LOCUS       DAOSYE010000145         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20572_length_3334_cov_3.010369, whole genome shotgun sequence.
ACCESSION   DAOSYE010000145 DAOSYE010000000
VERSION     DAOSYE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20572_length_3334_cov_3.010369"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLU43_03475"
     CDS             complement(<1..223)
                     /locus_tag="PLU43_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HOO27500.1"
                     /translation="MSEIKMIAPAVPNVPWQEKPENLEGAPIWRYSENPIVGRNPIEH
                     VARIFNSAVMPYEGAFIGVFRGEQTNGIPY"
     gene            complement(298..930)
                     /locus_tag="PLU43_03480"
     CDS             complement(298..930)
                     /locus_tag="PLU43_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27501.1"
                     /translation="MVRENLLYKIMSYIHIVLFVSLGCALTIVLSGSLLLLPSLAAVF
                     KIGREVLYKEADVTDSIVKKYFCCLKDSMKLMKFVGVDLIMLLNIASLYLFAGEQGIG
                     LSVAVLSIAAFLLVFQLYIAGFYVFVRKAFTLEEAAFAMLSRPMFLIPLFAAMVLLLF
                     FFNLTIAVILLFAGAFGIFALEVPVFIQMLGYKKMKGTLDESEKFYYLVK"
     gene            complement(950..1777)
                     /locus_tag="PLU43_03485"
     CDS             complement(950..1777)
                     /locus_tag="PLU43_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27502.1"
                     /translation="MDRESKGYKIFRYIFLILACVFVLIPIIPLVFMAFKTGAEYAST
                     PVLTPPKNWLNFYNFAYAIRVGELGQALVNTAIILTLALGIQLIFTTMVSYVLHRFTF
                     RGKKIITILFTLTMFIPCVTTQTVVFQMIYHLGLVNTRASVILLWAGVNIVGISIMFN
                     LLDSISKELDESALIDGASYFTIYRKIILPLLKPACTTLLILNGIGYYNDFYISNLYL
                     RSDIKTFTIALYKFFGNMATPFEIVSAAILIGIVPIGIVYLLLQKYIFYGLAGAIKS"
     gene            complement(1779..2309)
                     /locus_tag="PLU43_03490"
     CDS             complement(1779..2309)
                     /locus_tag="PLU43_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27503.1"
                     /translation="MNGVIVSTIFIIFFNNSGTLNTILSMIGLEGLTQQWLQNLKIVN
                     PAIASISIWRYYGMNFIMFFGALQTIPAELFEAAAVDGATKWQEIKFISIPSIKSVLY
                     INVLLSISGSIQVFEIPYIMMNGSNGTITPVIQIQQSAFQEDKYGFGAALSIVVFVIV
                     VIVVQLQNILRKRGEN"
     gene            complement(2266..2640)
                     /locus_tag="PLU43_03495"
     CDS             complement(2266..2640)
                     /locus_tag="PLU43_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27504.1"
                     /translation="MKRTKSARKVIILFLILPVIHLLVFSYIPILLNVYLSFTNYKGL
                     GTPKWIGLKNYRRIFTDTRYIAVFKNCLWYMLVAIPQLIFAFFLAVFVNGKFKGLNLF
                     KYSDHPLFIEWCNRFDDLYYFL"
     gene            complement(2723..>3334)
                     /locus_tag="PLU43_03500"
     CDS             complement(2723..>3334)
                     /locus_tag="PLU43_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27505.1"
                     /translation="NGFFEPEVYTDFGVAMDSVAYGKAAMMLFGSWVVPQIQGRVPEG
                     TDPSIIKFAPAPDFGDGRYVLAAPADNWAISKFSENKEAARAFIEYISEDAQFLADSG
                     YIANKKGVDPIVPDLYKIIDDAVEAGEVTPLFTPATDDNTLHNQEILEEAGLWADYKY
                     VGLLFDSLDVTKPDDWSAYEKQVEEQNQIYNDYRDELGYTWEE"
BASE COUNT         1007 a          812 c          617 g          898 t
ORIGIN      
        1 tataagggat cccatttgtc tgctctcccc tgaatacacc aataaaagct ccttcatacg
       61 gcatgaccgc actattgaaa attctcgcca catgctcgat cggattcctt cccacgatcg
      121 gattttcact gtagcgccag ataggggctc cttccaaatt ctccggtttc tcctgccatg
      181 gcacattcgg cacggccgga gcaatcattt ttatttcact catatgtcgt ctttctcctt
      241 catgttatgt tatatagttg cctggtctcc tggaaatcag gcaattttac agctttttta
      301 tttcacaagg taataaaatt tttcactttc atccagcgtt cccttcattt ttttataacc
      361 aagcatctgg ataaaaacag gtacttccaa agcaaatatt ccaaaggctc ccgcaaacag
      421 aagaataacc gctattgtaa gattaaaaaa gaacaaaaga agcaccattg ccgcaaacag
      481 cggtatcaga aacatcggcc gtgacagcat tgcaaacgct gcttcctcaa gtgtaaacgc
      541 tttccttaca aacacataaa agcctgcaat ataaagctga aaaaccagca aaaacgccgc
      601 aatcgaaagc actgccacgg aaaggccgat cccctgttct cctgcaaaca gatacaggct
      661 tgcaatattt aaaagcatga tcagatccac gccgacaaac ttcatcagtt tcatggaatc
      721 cttcagacag cagaaatatt ttttcacaat actgtctgta acatccgcct ctttatacag
      781 gacttccctg ccgattttaa acaccgcagc taaagaaggc agcagaagca gcgaacccga
      841 aagcacgatc gtcagcgcac agccaagact gacaaataat acaatatgga tataactcat
      901 gatcttgtac agtaaatttt ctcttaccat ttttattctc ctgctgtttt cagcttttga
      961 tcgcgcctgc cagtccgtag aaaatatatt tctgcagcag cagatatacg ataccgatcg
     1021 gaacgatgcc gatcaggatc gcagccgaca cgatctcaaa cggagttgcc atgtttccga
     1081 aaaatttata caaagcgatc gtaaatgttt tgatatcact tctaaggtac aggttgctga
     1141 tataaaaatc attgtaatac ccgatcccgt tcaggatcag cagcgtcgta caggcaggtt
     1201 ttaaaagcgg cagtatgatc tttctgtaga tcgtaaaata actcgcacca tcgatcagcg
     1261 ccgattcgtc gagttccttt gagatcgaat caagcagatt gaacataatg gaaatgccga
     1321 cgatattgac acctgcccag agcaggatga cgctcgctct tgtattgaca aggcccagat
     1381 gatagatcat ctgaaatacg accgtctgag tcgtaacaca tggaataaac atcgtcaggg
     1441 taaacaggat cgtaatgatc ttctttcctc tgaatgtaaa cctgtgcagc acataggaca
     1501 ccattgtcgt aaaaatcagc tggatcccaa gggccaatgt caaaataata gcagtattta
     1561 caagcgcctg cccaagctcc ccgactctga tcgcataggc aaaattataa aaattcagcc
     1621 agtttttcgg cggagtcagc accggtgtcg acgcatattc ggcacctgtt ttaaacgcca
     1681 taaaaactaa cggtatgatc ggaatcagca caaaaacgca ggcaagaatc aaaaatatgt
     1741 agcggaatat tttatatcct ttactttctc tgtccatttt aattctctcc tctcttcctt
     1801 aatatgtttt ggagctgtac aacaatcaca acgatcacaa acactacaat ggaaagcgcg
     1861 gctccgaagc cgtatttatc ttcctggaac gcactctgct ggatctggat gaccggagtg
     1921 atcgtcccgt tggacccatt catcataata taggggatct caaagacctg aatggagcct
     1981 gaaatactca gcagaacatt gatatacagc acgctcttga tggacgggat cgaaataaat
     2041 ttgatttcct gccattttgt cgcaccgtct accgcagccg cttcaaacag ctcggccggg
     2101 attgtctgca gcgccccgaa aaacataata aaattcatgc catagtaccg ccagatactg
     2161 atagacgcaa tcgccgggtt gacgatcttc agattctgca gccactgctg cgtcagtcct
     2221 tcaagaccga tcatacttaa aatcgtattc aatgttcccg aattattaaa gaaaataata
     2281 aagatcgtcg aaacgattac accattcaat aaataaggga tgatcagaat acttaaacag
     2341 attcagacct ttgaactttc cgtttacaaa taccgcaaga aaaaatgcaa aaatcagctg
     2401 tggaattgcc acaagcatat accacaaaca gtttttgaat acggcaatat atctcgtgtc
     2461 cgtaaaaatt ctccggtaat ttttcaatcc gatccatttc ggcgttccta atcccttgta
     2521 attcgtaaaa ctcaaatata cattcagtaa aatagggata taagaaaata ccagaagatg
     2581 aatgacgggc agtatcaaaa ataaaataat tacttttcta gcgctcttcg tccttttcat
     2641 atacggttcc tttctctcta aacaagtgcc tctccatgca gcgcagaaga ggcacttttc
     2701 ttaagattgt tatcctattt tactattctt cccatgtata accaagttcg tctctgtaat
     2761 cattgtagat ctgattctgc tcttcaacct gtttctcata agccgaccag tcatccggct
     2821 ttgtaacatc aagggagtca aataacagtc cgacatattt ataatcagcc caaagccctg
     2881 cttcctcgag gatctcctga ttgtgaagcg tattgtcgtc ggttgcaggt gtaaagagcg
     2941 gcgtaacttc tcctgcttct acggcatcat caatgatctt ataaagatca ggtacgatcg
     3001 gatccacacc cttcttgttt gcaatatatc cgctgtccgc aaggaactgt gcatcttctg
     3061 aaatatattc gatgaaagct cttgcagctt ctttattttc tgagaacttg gaaattgccc
     3121 agttatcggc aggtgccgca agtacatatc tgccgtcgcc aaaatcagga gccggtgcga
     3181 atttgataat ggaaggatcc gttccttccg gcacacgtcc ctggatctgg ggaacgaccc
     3241 atgagccaaa cagcatcatt gcagctttgc cataagcaac actgtccatg gctacaccga
     3301 aatccgtata aacttccggt tcaaaaaatc catt
//
LOCUS       DAOSYE010000146         3324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20659_length_3324_cov_3.610890, whole genome shotgun sequence.
ACCESSION   DAOSYE010000146 DAOSYE010000000
VERSION     DAOSYE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20659_length_3324_cov_3.610890"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLU43_03505"
     CDS             <1..427
                     /locus_tag="PLU43_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO27506.1"
                     /translation="FEVQAKPGVKNFLQNLRKHHIPMVIATTGDRELLKTALKRLSLE
                     PYFLEILTCTEFHTSKKYPYIYEKASDRLGTNPEQTFVFEDVLHAVASAAKGGFVTVA
                     VEDAASHADAEKIRETADYYMTDFLDFNHFWEFASKKHL"
     gene            505..1311
                     /gene="thiD"
                     /locus_tag="PLU43_03510"
     CDS             505..1311
                     /gene="thiD"
                     /locus_tag="PLU43_03510"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOO27507.1"
                     /translation="MKTTLTIAGSDSSGGAGIQADIKTMTMNGVYAMSAVTALTAQNT
                     TGVSGIMEVTPQFLAAQIDDIFNDIRPDAVKIGMVSSSALIGTIVDRLRFHKAENIVV
                     DPVMVATSGAKLISEEAILTLKEKLLPLASVITPNIPEAEVLSKMSIHTTDDMIAAAE
                     TIGKRHGCAVLCKGGHNLNNANDLLFANGQLHWFTGRRIDNPNTHGTGCTLSSAIASN
                     LAKGFDLFTSVERAKAYISGALEAMLDLGKGSGPMNHAFALTGEFAKEAR"
     gene            1313..2488
                     /gene="cytX"
                     /locus_tag="PLU43_03515"
     CDS             1313..2488
                     /gene="cytX"
                     /locus_tag="PLU43_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOO27508.1"
                     /translation="MENKQTSVLENSLIWFGAGVSIAEILTGTYFAPLGFKNGLLAIF
                     IGHIIGCTMLFLAGLIGGKVRKSAMETVKRSFGQKGSLLFSLLNILQLVGWTAIMIYD
                     GALAANNIWNGGRWIWCLVIGGLIILWILIGIKNLGKINTVAMTALFILTLILCSVIF
                     GSSSAAGITDEQLTFGAAVELSAAMPLSWLPLISDYTRNAARPIKATAASAIVYGLVS
                     CWMYVIGMGAAIYTGEYDIAGIMIKAGLGIAGLLIIVFSTVTTTFLDAYSAGVSSESV
                     FSKINGKWVAIGAAIIGTIGAIVYPMDNITNFLYLIGSVFAPMIAIQISDFFILKQDC
                     SKNQVNILNLIIWAAGFIIYRFLMHVDLPVGNTLPDMAITILLCIMIGSMKQRGTDK"
     gene            2485..3111
                     /locus_tag="PLU43_03520"
     CDS             2485..3111
                     /locus_tag="PLU43_03520"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOO27509.1"
                     /translation="MKRCVIIGGAKIGNYEFVRSYLKNDDFFVYCDSGLYHMEELRED
                     PDLVVGDFDSHPHPHLSAETITLPCEKDDTDTVFAVKETVSRGFSDFLLIGVTGGRID
                     HTLGNLSILLFLDSLKKTAVIVDDYSEMEIVSSSAAFVSDSFLYFSLLNISGTAKGIC
                     VEHAKYPLHDAEITCEFQYGISNEVLPGETAKISVREGRMLLIKIAAE"
     gene            complement(3114..>3324)
                     /locus_tag="PLU43_03525"
     CDS             complement(3114..>3324)
                     /locus_tag="PLU43_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27510.1"
                     /translation="CFYMMDGMLKKLTVALGETDETVYEFSEFKQEITDPAAFSYPEE
                     YTEESFDYVYSGESMPPWWEIGNDA"
BASE COUNT          911 a          756 c          792 g          865 t
ORIGIN      
        1 gtttgaagta caggcaaaac ccggagtaaa aaattttctg caaaacctgc gaaagcacca
       61 tattccgatg gtcatcgcca ctaccggaga ccgtgagctt ctcaaaacgg ctctgaaacg
      121 gctctctctt gagccatact ttttagagat actgacctgt acggaatttc atacgagtaa
      181 aaaatatccg tatatttatg aaaaagcctc agaccggctc ggcaccaatc cggaacagac
      241 ctttgttttt gaggatgttt tgcacgcagt ggcttcggcg gcaaaaggag gcttcgtaac
      301 agtggctgtc gaagatgcgg caagtcatgc ggatgcagaa aaaattcggg aaaccgcaga
      361 ttattatatg acggattttc ttgattttaa ccatttctgg gaattcgctt caaaaaagca
      421 cttataaatt tcagcggcag accgggggca ccaccttctg tcgcttaaca aaattaatgt
      481 tcatctgtgt aaaggagaaa aacaatgaaa acaacattaa caatcgccgg aagtgattcc
      541 agcggaggcg ccggtattca ggctgatatc aaaacaatga caatgaacgg tgtttatgca
      601 atgagtgctg ttacagcact gaccgcacag aacacaaccg gcgtatcagg gatcatggaa
      661 gtgaccccgc aatttttagc agcacaaatc gatgatattt tcaatgacat ccgacccgat
      721 gcggtaaaga tcggcatggt atcgtcaagc gccctgattg gaacgatcgt ggacagactt
      781 cgattccaca aagccgaaaa tatcgtagtc gatccggtaa tggtcgctac aagcggagca
      841 aagctcatca gtgaagaagc aatactgaca ctgaaggaaa aactgctgcc tcttgcctct
      901 gtcatcacac caaatattcc ggaagcggaa gttctgtcaa agatgtccat tcacaccaca
      961 gacgatatga tagcagccgc agaaacgatt ggtaaacgac acggatgcgc cgtattgtgc
     1021 aagggcggtc acaatttgaa taatgcaaac gacctgctgt ttgcgaacgg acaattacac
     1081 tggttcacag gcaggcggat tgacaatccg aatacgcatg gaaccggctg cacactgtcc
     1141 agtgcgatcg catcaaatct tgcaaagggc tttgacctct ttacttccgt cgaaagagcc
     1201 aaggcatata tatccggcgc tctagaggca atgctcgatc ttggaaaagg cagcggtccg
     1261 atgaaccacg cgtttgccct gaccggtgaa tttgcaaagg aggctcggta atatggaaaa
     1321 taaacaaacc tccgtattgg aaaacagcct gatctggttt ggcgcaggtg tttccattgc
     1381 agaaatactc acaggtactt attttgcacc gctcggtttt aaaaacggtc ttcttgccat
     1441 ttttatcgga catattatcg gctgcaccat gctgtttctc gccggactga tcggtggaaa
     1501 agtccgcaaa agcgcaatgg aaacagtaaa aagaagtttt ggtcaaaaag gcagtctgct
     1561 gttttccctg ctgaatattc tgcagcttgt cggctggacc gctattatga tctatgacgg
     1621 cgcactcgcc gcaaacaata tatggaacgg cggaagatgg atctggtgtc ttgtgatcgg
     1681 cggactgatt atcctgtgga tactgatcgg gatcaaaaat ctcggaaaaa tcaacaccgt
     1741 tgcaatgact gccctgttta tcctcacgct gatactctgc agtgtgatct ttggcagcag
     1801 ctccgcagcc ggtattacgg atgaacagct gactttcggt gcggccgttg aactttccgc
     1861 cgcaatgccg ctctcctggc tgccgcttat cagtgattat acgcggaatg ctgcgcgtcc
     1921 gataaaagcg actgcggcaa gtgcgatcgt ctatggcctt gtaagctgct ggatgtatgt
     1981 gatcggtatg ggcgctgcca tctatacggg tgaatatgac attgccggaa tcatgatcaa
     2041 agcaggactt ggcatcgcag gactcctgat tatcgtattt tccaccgtta cgacaacttt
     2101 tctggatgct tattcagccg gtgtgtcaag tgaatccgta ttttcaaaga tcaacggaaa
     2161 atgggttgcc atcggcgcag caatcattgg aacgatcggt gccatcgtct atccgatgga
     2221 taatattaca aacttccttt acctgatcgg atcggtattt gcacctatga tcgccataca
     2281 gatctctgat ttctttattt taaagcagga ctgcagcaaa aatcaggtga atattctgaa
     2341 tctgattata tgggcggcgg gctttatcat ttaccgtttc ctgatgcatg tggatctccc
     2401 tgtcggcaac acattaccgg acatggcgat cacgatcctg ctctgcatta tgatcggcag
     2461 tatgaaacaa cgaggtacgg acaaatgaaa agatgtgtga ttattggcgg agcaaagatc
     2521 ggcaactacg agtttgtacg cagttatctt aaaaatgatg atttttttgt ttactgcgac
     2581 agcgggcttt atcatatgga agaactgcgc gaagacccgg atctggtcgt tggggatttt
     2641 gattcacatc cgcatccgca tctgtctgcg gaaacgatca cgcttccgtg tgaaaaagac
     2701 gataccgata cggtttttgc cgttaaagaa acggtttcca gaggattttc cgacttcctg
     2761 ctgatcggtg ttaccggcgg caggatcgat catacgcttg gcaacctgtc gatcctgctc
     2821 tttcttgatt cgcttaaaaa aaccgccgta attgttgatg actattcgga aatggagatc
     2881 gtttcatcgt ccgcagcatt tgtcagtgat tcgtttctct atttttcact gctgaacatc
     2941 agcggtacgg ccaaaggcat ttgtgtggaa catgcaaaat atccgctgca tgatgctgag
     3001 atcacctgtg aattccagta tggcatcagc aacgaagtac tgcccgggga aactgcaaag
     3061 atcagtgtgc gggaagggcg aatgttattg ataaaaatag ctgcggaata aaactatgcg
     3121 tcatttccga tctcccacca tggcggcatg gattctccgg aatacacata atcaaagctt
     3181 tcttccgtat actcttccgg gtatgaaaaa gctgcagggt ccgtaatttc ctgtttgaat
     3241 tcggaaaatt catagactgt ttcatcggtt tctcccaatg cgacagtcag ttttttcaac
     3301 ataccgtcca tcatataaaa acaa
//
LOCUS       DAOSYE010000147         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20693_length_3320_cov_3.266462, whole genome shotgun sequence.
ACCESSION   DAOSYE010000147 DAOSYE010000000
VERSION     DAOSYE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20693_length_3320_cov_3.266462"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..2483
                     /locus_tag="PLU43_03530"
     CDS             192..2483
                     /locus_tag="PLU43_03530"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOO27511.1"
                     /translation="MQLEKNLEEITDHIFGKTIGACTDQEIYHCVLQLTKEMMETTEA
                     LSGDKKVYYISAEFLIGKLLSNNLINLGIYDKLEDILKKNGKQLSVIEDMEPEPSLGN
                     GGLGRLAACFLDSIATLGLQGDGIGLNYHFGLFKQIFSDRLQQTEKNEWIEKDSWLTK
                     TNTSFDVWFGDKKVRSVLYDMNVIGYQNGLNKLHLFDIESVDESIVGEGISFDKEDIE
                     KNLTLFLYPDDSDEAGNLLRIYQQYFMVSSAAQLILKEMREKQYDLRQMYKHAVIQIN
                     DTHPTMIIPELIRILTKDKAISMDDAIEIVSKTCAYTNHTILAEALEKWPLAYLEKVV
                     PQLVPIIKELDRRVAEKYSDKKVQIIDDEKRVHMAHIDIHYGFSINGVAALHTEILKE
                     SELKHFYDIYPEKFNNKTNGITFRRWLIACNRELSDYISEVIGDYWKKDAVRLEGLLE
                     HLGEQEVLDRIFSIKQQKKKELAEYIEKTEGVTLDMNSIFDIQAKRMHEYKRQQMNAL
                     YIIHKYLEIKAGEIPARPMTFIFGAKAAPAYVIAQDIIHLLLVLQEVINHDKKVSPYM
                     KVLMVENYNVSYAEKLIPACDISEQISLASKEASGTGNMKFMLNGAITLGTSDGANVE
                     IHQLVGDENIYIFGKDSETVIRHYEKADYVSKDYYKKSPVIKEAVDFIVSRKMLRAGH
                     KENLKRLHKELLNKDWFMTLLDLEEYIAVKDRAFADYEDQASWKKKMLVNIAKAGFFS
                     SDRTIEEYNRDIWKLNDWEKIEK"
     gene            2771..>3320
                     /locus_tag="PLU43_03535"
     CDS             2771..>3320
                     /locus_tag="PLU43_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27512.1"
                     /translation="MKKKVISMVLAVTMVASLLAGCGSAATETEEAAPAAQEETEQTE
                     EAAPAEEASAEDTEAADAEGGKVYYLNFKPEQAEQWVALGETYEEQTGVPVTIETAAS
                     GTYESTLKSEMAKTEAPTLFQVNGPVGLANWQDYTYDLQDSDVYAQLKSDDFALKGDN
                     GEVYGIAYVIETYGIIYNKKLIA"
BASE COUNT         1136 a          546 c          787 g          851 t
ORIGIN      
        1 aataaaaata aaaaaattaa aaaagatgtt gcggaaaacg ttttcctaaa ttatgataaa
       61 catataaaga aaaatacccg atggacggcg gatcttattt aggaatgaaa gaactgccat
      121 ttatgaaaat aatgtttgga atggaggata aaaatatgtc atatcaaaaa gtcaggacgg
      181 aggtactgca aatgcagctg gaaaaaaatc tggaagagat cacagatcat atttttggaa
      241 aaacaatagg ggcgtgtacg gatcaggaaa tctatcattg tgtgcttcag ctgacaaagg
      301 agatgatgga gactacagaa gcgctatctg gtgataaaaa agtctattat atttcggcag
      361 aatttctaat cggaaagctg ttgtcaaata atctgatcaa tttgggaatt tatgataagc
      421 tggaggatat tcttaaaaag aatggaaagc aactttctgt tattgaagat atggaaccgg
      481 aaccatcgct tggaaatgga ggacttggaa ggctcgctgc ctgttttttg gattctattg
      541 ccacgcttgg actgcagggc gatggtattg gcctgaatta tcattttggc ctgtttaaac
      601 agattttttc tgacaggctg cagcagacgg aaaaaaatga atggatcgag aaagattcat
      661 ggcttacaaa gacgaatact tcttttgatg tatggttcgg cgataaaaaa gtcagatccg
      721 tgctctatga tatgaatgtg atcggctatc agaatggcct taataaatta catctgtttg
      781 acatcgaatc tgtagatgag agtattgtcg gggaagggat cagctttgat aaagaagata
      841 ttgaaaagaa tctgacgctg ttcctgtatc cggatgattc ggatgaggca ggtaatcttc
      901 ttagaattta tcagcagtat tttatggtaa gcagcgcagc acagcttatc ctgaaggaaa
      961 tgagggaaaa acaatatgat ctgcgacaga tgtataaaca cgcagtcatt cagattaatg
     1021 atacgcatcc gaccatgatc attccggaac tgatcaggat cctcacaaag gacaaggcta
     1081 tttccatgga tgatgcaatc gagatcgtaa gtaaaacgtg tgcatacacg aatcatacga
     1141 tactcgcaga agcgttggaa aaatggccgc tcgcctatct tgagaaagtc gttccgcagc
     1201 tcgttccaat tattaaggag ctggatagga gagttgcaga aaaatatagt gataaaaaag
     1261 tgcagatcat tgatgatgag aagagagtcc atatggcaca cattgatatc cattatggat
     1321 tttccataaa cggagtggct gcgctgcaca cagaaattct taaagaatca gaattaaaac
     1381 atttttatga tatttatcct gaaaaattca ataataaaac aaatggaatt actttccggc
     1441 gctggctgat tgcctgtaat cgggaacttt ccgattatat ttccgaagtg atcggtgatt
     1501 actggaaaaa agatgcagtc cggctggaag gactgcttga gcatctgggt gagcaggaag
     1561 tccttgaccg tattttttcg atcaagcaac agaaaaagaa agaactggca gaatatattg
     1621 aaaaaacaga aggcgtcacg cttgatatga attcgatttt tgatattcag gcaaaacgga
     1681 tgcatgagta taaacgtcag cagatgaatg cactttatat cattcataaa tatttagaga
     1741 tcaaagctgg agaaataccg gccagaccaa tgacatttat ttttggggca aaggcagcac
     1801 ctgcttatgt gatcgcgcag gatatcattc acctgctgct cgttctgcag gaagtcatca
     1861 atcatgataa aaaggtcagc ccgtatatga aggtactgat ggtggagaat tataatgtca
     1921 gttatgcaga aaaactgatc ccggcctgtg atatttctga acagatctca cttgcctcca
     1981 aggaagcatc cgggaccggc aatatgaaat ttatgctgaa cggtgcgatc acacttggta
     2041 cgagcgatgg tgcaaatgtg gagatccatc agctagttgg tgatgagaat atttatatat
     2101 tcggtaaaga ctctgagacc gtgatcagac attatgaaaa agcagattat gtatcgaagg
     2161 attattataa aaagagtcct gtcatcaagg aagcggtaga ttttatcgtg agcagaaaaa
     2221 tgctgcgggc cggacataaa gaaaatctga aaagactgca taaggagctt ttgaataaag
     2281 actggtttat gacacttctt gatctggagg aatatattgc cgttaaagat cgtgcatttg
     2341 ctgattatga agatcaggct tcatggaaga aaaagatgct tgttaatatt gcaaaggccg
     2401 gattcttttc atctgacagg acgatcgaag aatataatcg tgatatctgg aagctgaatg
     2461 actgggaaaa aatcgaaaaa taacaaaaag acaaagcccg gcagctgccg ggctttagta
     2521 aaaatatata aaatcctact gcctgtttga tataaagtgt acaaagttat aagaagggta
     2581 ttgcattatc tggagacagg tgatactatt taaacaagga accggaaacg ttaccgacat
     2641 cgttaccgat accgttaccg ttagcgatca taacgatcca gtaactgtta tggcagaatg
     2701 tttctgcgaa acttaatcat cagcctctga tagcgctatt acagaggcaa aatttaaaag
     2761 gagaggtaat atgaaaaaga aagtaatcag tatggtactt gcagtaacaa tggtggcttc
     2821 tttactggct ggttgcggat ctgctgcaac agagacagaa gaggcagcac cggcagcaca
     2881 ggaagaaaca gaacagacag aagaggcagc accggcagaa gaagcatctg cggaagatac
     2941 ggaagctgct gatgcagaag gtggaaaggt ctattatttg aatttcaagc ctgagcaggc
     3001 agaacagtgg gtagctttgg gagagaccta tgaagagcag acaggtgttc ctgtaacaat
     3061 tgaaacagca gcatccggaa catatgaatc aacactgaaa tcagagatgg caaaaacaga
     3121 agcacctaca ttatttcagg taaatggacc ggtaggactt gcaaattggc aggattacac
     3181 gtatgattta caggacagtg atgtatacgc acagttaaag agcgatgatt ttgccctgaa
     3241 gggagataac ggcgaagtat acggaatcgc ctatgtaatc gagacctatg gcatcatcta
     3301 caataaaaag cttattgcag
//
LOCUS       DAOSYE010000148         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20712_length_3318_cov_2.920932, whole genome shotgun sequence.
ACCESSION   DAOSYE010000148 DAOSYE010000000
VERSION     DAOSYE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_3318_cov_2.920932"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1051
                     /locus_tag="PLU43_03540"
     CDS             173..1051
                     /locus_tag="PLU43_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOO27513.1"
                     /translation="MIKSMTGFGRCEVSDESRKITVEMKAVNHRYLDINIKMPKKLSF
                     FEASMRNLLKEYIQRGKVDIFIVYEDYTENNYSLKYNREMAAAYLQYIEKMASDFSIE
                     NDIRASVLSRYPEVLTLEEQAFDEKGLWSDLEKALRGAAEAFVLTRITEGENLQNDLT
                     QKLDGMLAHVDYIAERAPEIIAEYRTKLTEKVTEFLADAKIDESRILTEVALFSDKIC
                     VDEEIVRLRSHIEHTKATLNEGGSIGRKLDFIAQEMNREANTILSKANDLTTSNRAID
                     LKTDIEKVREQIQNIE"
     gene            1071..1703
                     /gene="gmk"
                     /locus_tag="PLU43_03545"
     CDS             1071..1703
                     /gene="gmk"
                     /locus_tag="PLU43_03545"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO27514.1"
                     /translation="MKERGILLVVSGFAGSGKGTLMKELLKRYDNYALSVSATTRSPR
                     PGEINGVDYFFVTTENFENMVAKNELLEHAKYVSNYYGTPRGYVEEQLQAGKDVLLEI
                     EIQGALIIKEKYPEALLLFVIPPSVQEVYGRLKKRGTETKEVIASRMRRAKEEAEMIG
                     RYDYLIINDDLDTCVQTLHHVVMSAHCAVFRNYDTINKIKRQFVDFLEGE"
     gene            1708..1947
                     /gene="rpoZ"
                     /locus_tag="PLU43_03550"
     CDS             1708..1947
                     /gene="rpoZ"
                     /locus_tag="PLU43_03550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOO27515.1"
                     /translation="MLHPSYTDLMKVVNSEVEEGERPIVNSRYSIVMATSKRARQLIA
                     GAPTPVESAKEKKPLSVAIEELNDGIIKVVGDEAK"
     gene            1944..3287
                     /gene="rimO"
                     /locus_tag="PLU43_03555"
     CDS             1944..3287
                     /gene="rimO"
                     /locus_tag="PLU43_03555"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOO27516.1"
                     /translation="MKLLFISLGCDKNLVDTEIMLGILQQYSFEFTDDEAEADVIIIN
                     TCCFIHDAKEESIHSILQMAEYKEENGGKCKALLVTGCLAQRYADEIRKEIPEVDAIV
                     GTTAYDQIAEALSAVLEGKEGDRMASIEALPIIEGRRVITTGGHFAYLKIAEGCDKHC
                     TYCIIPKLRGHFRSVPMEVLLREAERLVNAGVKELILVAQETTLYGVDLYGGKSLPKL
                     LKKLCRIEGLFWIRILYCYPEEITDELIQTIKEENKICNYLDIPIQHASDAVLKRMGR
                     RTDKRQLKEIIGKLRKEIPDICIRTTLITGFPGETKEEHQELMEFTDEMEFERLGVFT
                     YSQEEDTPAAAMPLQIEEEVKQDRQAQLMEQQQEIAFLHAEDMIGRELDAFVEGKVID
                     EEAYAARTYMDAPNIDGLLFIHTSKELMSGDMVKVRVTGAYEYDLIGELCNESAK"
BASE COUNT         1032 a          568 c          893 g          825 t
ORIGIN      
        1 gtcttttatt tttagaatcg tatcatgaca agacacttta tgatacagag gttattataa
       61 aagaattttt tcagaaaagc aatggcttgc ttgacgttgc agaaacgatg gactataata
      121 aaaaatagta tgcggctggg gctgaggctt tagccaaagg agaaaatcgt atatgataaa
      181 gagcatgaca ggcttcggac gttgtgaggt ttctgatgaa agcagaaaaa tcacagttga
      241 gatgaaagcg gtaaatcaca gatatcttga tattaatatt aagatgccaa agaaacttag
      301 tttttttgag gcatccatgc gtaatctttt aaaagaatat atccagcgcg gaaaggtgga
      361 tatttttatt gtttatgaag attatacaga gaataattac agcctgaaat ataacaggga
      421 gatggcggct gcatatctgc agtatattga aaaaatggca tctgattttt caatagaaaa
      481 tgatatccgg gcgtccgtcc tgtccagata tccggaagtc ctgacgctgg aagagcaggc
      541 atttgacgaa aagggacttt ggagtgatct ggaaaaggct cttcgggggg ctgcagaagc
      601 gtttgttttg acgaggatca cggaagggga aaatctgcag aacgatttga cacaaaagct
      661 tgacgggatg cttgcccatg tggattacat agccgaaaga gcaccggaga tcatagctga
      721 ataccgtaca aagctgaccg aaaaggtaac ggagtttctg gcggacgcaa aaatagacga
      781 gagccgtatt ctgactgaag ttgctttgtt ttctgataaa atctgtgttg atgaagagat
      841 tgtcaggctg cgaagccata ttgagcatac gaaggctacg cttaatgagg gcggaagtat
      901 tggcagaaag cttgatttta ttgcacagga aatgaatcgc gaagcaaata cgattttatc
      961 aaaagcgaac gatctgacga cttcgaacag ggcgatcgat ctgaaaaccg atattgaaaa
     1021 ggtcagggaa cagatacaga atatcgagta aatcaagcgg agagagtttt atgaaagaaa
     1081 gaggaatact tttggtagta tccggatttg cgggttccgg caagggaacg ctgatgaagg
     1141 aacttttaaa acggtacgat aattatgcgc tgtcggtttc ggcaacgacc aggagcccca
     1201 gacccggtga aataaatggt gttgactatt ttttcgtgac aacggaaaat tttgaaaata
     1261 tggttgcaaa gaatgaactt ttggagcatg caaaatatgt ttccaattat tacggaactc
     1321 cgcgcggata tgtggaagag cagcttcagg caggcaaaga tgttttactc gaaatagaaa
     1381 tacagggagc tttgatcatt aaagaaaaat atccggaggc attgctgctg tttgtgattc
     1441 cgccgagtgt gcaggaagtt tacggccgcc ttaagaagcg ggggaccgag actaaggagg
     1501 tgatcgcttc ccgtatgcgc agggcaaagg aagaggccga aatgatcggg aggtatgatt
     1561 atctgatcat caacgatgat ctggatacat gtgtgcagac actgcatcat gttgtaatga
     1621 gtgctcattg tgcggttttc cgcaattatg atactattaa taaaattaaa cgccaatttg
     1681 ttgatttttt ggaaggagaa taaatttatg ttgcacccgt cttatacgga tttaatgaaa
     1741 gtagtgaata gtgaagtcga ggaaggagaa cggccgatcg taaacagccg ttattccata
     1801 gtaatggcca cttcgaaaag ggcaagacag ctgatcgccg gcgctccgac accggtagaa
     1861 agtgcaaaag agaaaaagcc gctgtcggtt gccattgaag aattaaatga cggtattatt
     1921 aaggtcgtcg gcgatgaagc taaatgaaac tgttatttat ttcgctgggc tgtgataaaa
     1981 atctggttga tacggagatc atgcttggta ttctgcagca atattccttt gagtttactg
     2041 atgatgaagc agaagcagat gtgattatta ttaacacatg ctgctttatt catgatgcga
     2101 aagaagaaag tatccattcg attctgcaga tggctgagta taaagaggaa aacggcggaa
     2161 agtgtaaggc tctgcttgtg acgggctgtc ttgcacagcg ctatgccgat gaaatcagaa
     2221 aagagattcc ggaagttgac gcgatcgtcg gaacgacggc gtatgatcaa attgccgagg
     2281 cactgtctgc cgtacttgaa ggcaaagagg gcgatcgtat ggcaagcata gaggcacttc
     2341 cgatcataga aggccggcgg gtcataacga caggggggca ttttgcctat ctgaagattg
     2401 cggaaggctg tgacaagcat tgtacctact gtattattcc aaagctgcgc gggcatttca
     2461 gaagcgttcc aatggaagta ctgctcagag aggcagagcg tcttgtaaat gccggtgtaa
     2521 aagagctgat tcttgttgcg caggaaacaa cactctatgg tgttgatctt tacggcggga
     2581 agtccctgcc aaagctgctg aaaaagcttt gtaggataga agggctgttc tggatacgga
     2641 ttctctactg ttatccggaa gagatcaccg atgaactgat acagacgatc aaggaagaaa
     2701 acaagatatg caattatctg gacattccga tccagcatgc ttccgatgcg gttttgaaac
     2761 gaatgggaag acgtacggac aaacgtcagc tgaaagagat tatcggcaag ctgcgaaaag
     2821 agattcctga tatctgcatt cggacgaccc tgatcacagg atttcctgga gaaacaaaag
     2881 aagagcatca ggagcttatg gaatttacgg atgaaatgga attcgagcgg cttggggtat
     2941 ttacctattc gcaggaagag gatacgccgg ctgccgcgat gcctttacag atagaggaag
     3001 aagtaaaaca ggacaggcag gcacagctca tggaacagca gcaggaaata gcgtttctgc
     3061 atgccgaaga tatgattggg cgtgaactgg atgcatttgt ggaaggaaag gtaattgacg
     3121 aagaggcgta tgcggcgcgt acgtatatgg atgcgccgaa tattgacggc cttttgttca
     3181 tacatacatc aaaagagctg atgagcggag acatggtaaa ggtcagagtc accggtgcct
     3241 acgaatatga tctgatagga gagttatgta atgaatctgc caaataaact gacgctgctt
     3301 cgggtattga tgataccg
//
LOCUS       DAOSYE010000149         3314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20747_length_3314_cov_3.061675, whole genome shotgun sequence.
ACCESSION   DAOSYE010000149 DAOSYE010000000
VERSION     DAOSYE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20747_length_3314_cov_3.061675"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="argJ"
                     /locus_tag="PLU43_03560"
     CDS             <1..942
                     /gene="argJ"
                     /locus_tag="PLU43_03560"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOO27517.1"
                     /translation="AKAAGNLLHIPQDAVLVGSTGVIGMQLPMDKITAGVEKLVPLLE
                     ATLEAGQQAAEAIMTTDTVRKEAAVQVEIGGKTVTVGGMSKGSGMIHPNMCTMLCYVT
                     TDAAISKELLTEAVKTDVIDTFNMISIDGDTSTNDTLLVLANGMAGNKEITEKNKDYA
                     VFCEALHYINETLAKKMAGDGEGATALFTATVTGAKTKQDARMLAKSVITSSLTKAAI
                     FGHDANWGRILCALGYSGVQFDPEKIELTFMSDAGKLLIFKDGLATDYSEEEATKILS
                     SKEVTVLVDMKLGDKAATAWGCDLTYDYVKINADYRS"
     gene            966..1862
                     /gene="argB"
                     /locus_tag="PLU43_03565"
     CDS             966..1862
                     /gene="argB"
                     /locus_tag="PLU43_03565"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOO27518.1"
                     /translation="MDEIYMEKYLHKAEVLIEALPYIQKFNRKVIVVKYGGSAMTDEE
                     LKKNVIKDVTLLKLVGFKPIVVHGGGKEISRWVGKIGKEAKFVNGLRVTDEETMEIAE
                     MVLGKVNKSLVAMVQELGVRAVGISGKDGGLLKVRKKYADGEDIGYVGEITEVNTKIL
                     EDLLENDFLPIIAPVGLDEDFLTYNINADDAACAIAKAMHADKLAFLTDIEGVFQDPA
                     DKETFISRLTVSEANALIKDGYIGSGMLPKINNCTEAITSGVNRVHILDGRKPHCLLL
                     EIFTNKGIGTMFFPDEEKGTLL"
     gene            1859..3058
                     /locus_tag="PLU43_03570"
     CDS             1859..3058
                     /locus_tag="PLU43_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOO27519.1"
                     /translation="MTMGQYIQEAESVLLHTYNRYPIVLDRGEGVYLYDIEGKKYLDF
                     CAGIAVFALGYGNKSYNDALKEQIDKLLHTSNYYYTVPAVRAAQKLAEASGMDRVFFT
                     NSGAEAIEGAIKAARKYAYMKDGSNDHEMIAMENSFHGRTMGALSVTGNPKYREAFYP
                     LIGNINYAKYNDIDSVKALLSDKTCAIILETVQGEGGIHPAEKEFLKEIRRLCDENGM
                     LLILDEIQCGMGRTGRMFAYEHYGVMPDIMTVAKAVGGGVPVGAFLMTQKVADQSLAA
                     GDHGTTYGGNPFVTAAVNAVFDQFKEKEILSNVCEVAPYLEDRLDMLIRKYDFLTGRR
                     GIGLMQGLVFDTGKMLPADVSKRALDNGLVIITAGADVLRFLPPLIITKADVDEMLVR
                     LEKSFMI"
     gene            3086..>3314
                     /locus_tag="PLU43_03575"
     CDS             3086..>3314
                     /locus_tag="PLU43_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27520.1"
                     /translation="MNKNRFSAILSFVLVAAMLVISLRDSNPLSDRLVSTKLSVLTGS
                     DYMENMESADTTETAEETADTQLADDDTLILW"
BASE COUNT          953 a          622 c          954 g          785 t
ORIGIN      
        1 gcaaaggcag cagggaacct gctgcatatt ccgcaggatg cggtcctggt gggctctacc
       61 ggagtgatcg gcatgcagct gccaatggat aagatcacag ccggggtgga gaagctggtg
      121 ccgctgctgg aagcgacttt ggaagccggg cagcaggcgg cggaagcaat catgacaaca
      181 gatacggtca gaaaggaagc ggcagtacag gttgagatcg gcggaaagac agtaacggtc
      241 ggaggcatga gcaagggttc cggtatgatc catccaaata tgtgtacgat gctctgctat
      301 gtaacaacag atgcggccat ttccaaggaa cttctgacag aagcggtaaa gaccgatgtg
      361 attgatacgt tcaatatgat ttccatagac ggggatacat caacaaacga tacattgctc
      421 gtactggcga acggtatggc cggcaataag gaaattacgg aaaaaaataa agactatgcg
      481 gttttctgtg aagcgctgca ctatattaat gaaacacttg caaaaaaaat ggccggtgac
      541 ggggaaggtg caacggcgtt gtttacggca acggtgacgg gagcaaagac gaagcaggac
      601 gcaagaatgc ttgcaaaatc cgtaatcacc tcgtctctca caaaagcggc aatattcgga
      661 catgatgcca attggggcag gatcctctgc gcacttggtt attccggtgt tcaatttgac
      721 ccggagaaga tcgaacttac ttttatgtcc gacgcaggaa aacttttgat attcaaagac
      781 gggcttgcaa cggattacag cgaagaggaa gcgacaaaga tcctttcttc aaaagaggtg
      841 acggtccttg tcgacatgaa gcttggagat aaagctgcta ccgcatgggg ctgcgatctg
      901 acctatgatt acgtaaaaat caatgccgat tacagaagct gaaggaggaa tgaattgtga
      961 gcagcatgga tgaaatatac atggaaaaat atttgcacaa ggcggaggtt ctgatcgaag
     1021 cacttcctta tatacaaaag ttcaaccgca aggttatcgt tgtcaagtac gggggaagcg
     1081 caatgacgga cgaagaactg aaaaagaatg tgatcaagga cgtgacgctg ctgaagcttg
     1141 tcggttttaa accgatcgtt gtacatggcg gcggcaagga gatcagccgc tgggttggaa
     1201 agataggaaa agaagcaaag ttcgtgaatg gtctgcgggt cacggatgaa gagacaatgg
     1261 aaattgcgga gatggtgctt gggaaagtca ataaatcgct ggttgccatg gtgcaggaac
     1321 tgggcgtcag ggcggtcggc atttccggca aggacggagg actgctgaag gttaggaaga
     1381 aatatgccga cggtgaggat atcggttatg tcggcgagat cacggaagtc aatacaaaaa
     1441 tactggaaga tctgctggaa aacgattttc ttccgatcat agcgccggtc ggcctggacg
     1501 aggacttcct gacatataat atcaatgcgg atgatgcggc ctgtgcgatc gcaaaggcaa
     1561 tgcatgcgga taagctggcg tttcttacgg atatcgaagg cgtttttcag gatccggccg
     1621 ataaggagac atttatctcc cgtttaacag tatctgaagc gaacgcattg atcaaagacg
     1681 gttatatcgg cagcggcatg cttccaaaga tcaataactg tacggaggcg atcacaagcg
     1741 gtgtgaaccg cgttcatata ctcgacggga gaaagccgca ctgtctgctg ctggagatat
     1801 ttacgaataa agggatcgga acgatgtttt ttccggacga agaaaagggg acactgctat
     1861 gacaatggga caatatattc aggaagcgga aagtgttctg cttcatacgt ataaccggta
     1921 tccgatcgta ctcgacaggg gcgaaggtgt ttatttatat gacattgagg gaaagaaata
     1981 tctggatttc tgcgcaggaa ttgcagtatt tgcacttggt tatggaaata aaagctataa
     2041 tgatgcactg aaggaacaga tcgataagct gctccataca tctaattatt attataccgt
     2101 accggcagtc agggcggcac agaaattggc agaagcttcc ggaatggatc gggtattttt
     2161 tacgaacagc ggggccgaag caattgaagg agccattaag gctgcccgga aatatgctta
     2221 tatgaaggat ggcagtaatg atcatgaaat gattgcgatg gagaattcct tccatggacg
     2281 taccatggga gcgctttcag taactggaaa tccaaaatac agggaagctt tttatccgct
     2341 gatcggaaat atcaattatg cgaagtacaa tgatatcgac agcgtaaagg ctttgctttc
     2401 agacaagacc tgtgctatta ttttagagac ggtccaggga gaaggcggga tccatccggc
     2461 agaaaaagaa tttttaaaag agattcgcag gctttgtgat gagaacggga tgctgctgat
     2521 actggatgag atccaatgcg gtatggggcg taccggcagg atgtttgcat atgagcatta
     2581 tggtgtgatg ccggatatta tgactgttgc aaaggcagtc ggcggcggag tgccggtcgg
     2641 agcgtttctg atgactcaga aagttgcgga tcagtccctg gctgcaggtg atcatggtac
     2701 gacatacggc ggcaatccgt ttgtaactgc ggcagtcaat gcagtatttg accagtttaa
     2761 ggagaaagaa atactttcta atgtatgtga ggtcgctccg tatctggagg acaggctgga
     2821 tatgcttata cggaagtatg attttctgac aggacgccgc gggatcggac ttatgcaggg
     2881 gcttgttttt gatacgggga agatgctgcc ggctgatgtc agcaaaagag cgcttgataa
     2941 cggacttgtt attattacgg ccggagcgga tgtgcttcgt tttctgccgc ctttgatcat
     3001 tacgaaggca gacgtggatg aaatgcttgt tcgtcttgaa aaatcattta tgatataggc
     3061 tgtaagatag ttccggggga atggtatgaa taaaaatcgg ttttcggcaa ttttatcatt
     3121 tgtattggtg gcggcaatgc ttgtgatatc actgagggac agtaatccgt taagtgaccg
     3181 gcttgtctcg acaaagctgt ccgtattgac aggatcggat tatatggaaa atatggagtc
     3241 tgcagatacg acggaaactg cagaagaaac tgcggatacc cagctggctg atgatgatac
     3301 gctgatcctc tggt
//
LOCUS       DAOSYE010000150         3314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20748_length_3314_cov_2.914391, whole genome shotgun sequence.
ACCESSION   DAOSYE010000150 DAOSYE010000000
VERSION     DAOSYE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20748_length_3314_cov_2.914391"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLU43_03580"
     CDS             <1..204
                     /locus_tag="PLU43_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27521.1"
                     /translation="PNRKGTLTKSLLHENVAYTIYEGIPLTGWPVMTLSRGDIIVKDG
                     VYVGERRRGQFIKREKGCFTGSR"
     gene            297..1739
                     /gene="glgA"
                     /locus_tag="PLU43_03585"
     CDS             297..1739
                     /gene="glgA"
                     /locus_tag="PLU43_03585"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOO27522.1"
                     /translation="MKKILFAASEGVPFIKTGGLADVVGSLPKCFDKKYYDVRVVMPK
                     YLCIGQEYRDQMTYIDHFYMDIGSRSQYVGIFELACEGIIFYFIDNEYYFSETKPYGD
                     LLWDIEKFSFFSKAALSILPIIGFQPDIVHCHDWQTGLIPVYLKERFHDGEFFRSMKS
                     VMTIHNLKFQGIWDVKTMKELTGLPDYFFTPDKLEAYKDGNLLKGGIVFADAITTVSN
                     TYAEEIKMPFYGEGLDGLLRSRNSQLRGILNGIDYEEYNPLTDKYIVSPYHAKDFRKE
                     KIKNKRALQAELGLEQDDKKFMIGIVSRLTDQKGFDLIVHVMDELCLMDVQIVILGTG
                     EERYENMFRHFDWKYDNKVSANIYYSEEISHKIYASADAFLMPSLFEPCGLSQLMALR
                     YGTIPIVRETGGLKDTVEPYNEYESSGTGFSFCNYNAHEMLNTIRYAQSIYANKKREW
                     NKMIDRAMAADFSWKASALKYQEMYDWLVP"
     gene            complement(1777..>3314)
                     /locus_tag="PLU43_03590"
     CDS             complement(1777..>3314)
                     /locus_tag="PLU43_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOO27523.1"
                     /translation="SPMTAPNFCMLLRKHLQNGRIVSIRQPGLERIIMFEIEHLDEMN
                     DVCHKTLVVEIMGKHSNIIFLDDHHTVIDSIKHISGAVSSVREVLPGRDYFIPMTQKK
                     YDPFEISKETFLSQIAAKPMPAAKAVYTTLTGFSPVMAEELLFRASIDSSLPANTLSD
                     EHLLHLYNHFSWLMEEVSEKRFKPCIFLLEDNPLEFAALPLTQYGDMKTLENPSISYI
                     LETYYAKKNTITRIRQKSADLRRIVQTALERNTKKYELQLKQLNDTQKKDKFRIYGEL
                     LNTYGYGLKTGCKSFEADNYYTGETITIPLNETLSPQENAKKYFDKYNKLKRTEEAVN
                     IQSAETKKEIEHLESIGTALDIALYEEDLAEIKDELVESGYIRRKSTMKKKKFTSRPL
                     HYISSDGYQIYVGKNNYQNDDLTFKFAVGNDWWFHAKGIPGSHVIVKSNGEDLPDKTF
                     EEAGSLAAYYSKGREQGKVEIDYVQKKNIKKPNGSKPGFVVYYTNYSLVAVPDISRIT
                     QVL"
BASE COUNT          901 a          631 c          779 g         1003 t
ORIGIN      
        1 ccgaacagaa aagggacgct tacaaaatcc cttcttcatg aaaatgttgc gtatacgatc
       61 tatgagggga tcccgcttac aggatggcct gtgatgacgc tctcaagggg agatatcatc
      121 gtaaaagacg gtgtatatgt cggagaacgc aggagaggac aatttattaa acgggaaaaa
      181 ggatgcttta caggaagccg gtaaggcttc cttttttaca aattgtgtct tttctttgac
      241 atgattttcg tgtataatat taagtaatat atattccgtg agggaggttt caatgtatga
      301 aaaaaatctt atttgccgca tcagagggtg ttccctttat caagacaggc ggtctggcag
      361 atgtggttgg ttccctgcca aaatgttttg ataaaaaata ctatgatgtt cgcgttgtta
      421 tgccaaagta tttatgcatc ggccaggagt acagggatca gatgacatat atcgaccatt
      481 tttatatgga tatcggcagc agatctcagt atgtgggtat ttttgaactg gcctgtgagg
      541 gcattatttt ttattttatc gataatgaat attatttttc cgagaccaaa ccatatgggg
      601 atctgctgtg ggatatagag aaattttctt ttttttccaa ggcagcactt tccatacttc
      661 caatcatagg atttcagcct gatattgttc attgtcatga ctggcaaacc ggtctgattc
      721 cggtatattt aaaagaacgg ttccatgatg gggaattttt cagaagtatg aagtctgtta
      781 tgacgatcca taatctgaaa tttcagggta tctgggatgt aaaaacaatg aaggaattaa
      841 ccggactgcc cgattatttt ttcacaccgg acaagctgga ggcatataaa gacgggaatc
      901 tgctgaaagg cgggatcgta tttgcggatg cgatcacaac ggtcagcaat acatatgccg
      961 aagagataaa aatgccgttt tacggagaag gtctggacgg cctgcttcgc tccagaaaca
     1021 gtcagctgcg cggaattctt aacgggatcg attatgagga atataatcct ttgacagata
     1081 aatatattgt cagtccttat catgcaaagg atttcagaaa agaaaagatc aagaataagc
     1141 gggctttgca ggcagagctt ggacttgagc aggatgataa aaagtttatg atcggtattg
     1201 taagccgtct gacggatcag aaagggtttg atctgattgt tcatgtgatg gatgaactct
     1261 gtctgatgga tgtacagatc gtgattcttg gtaccggtga agagcggtat gaaaatatgt
     1321 tccggcattt tgactggaaa tatgacaata aagtatctgc caatatctat tattcggaag
     1381 aaatttccca caagatctat gcctccgccg atgcgttttt gatgccgtcc ctgtttgaac
     1441 catgcggatt atcccagctt atggcactcc gttacgggac gattccgatc gtcagggaaa
     1501 caggcggtct gaaagatacg gtagagccgt ataacgaata tgagagcagt ggtaccggat
     1561 tcagtttctg caactataat gcacatgaga tgctcaatac gatacggtat gcgcagagta
     1621 tttacgcgaa taaaaaaaga gaatggaata agatgatcga tcgtgcgatg gcagctgatt
     1681 tttcatggaa ggcatccgca ctgaaatatc aggaaatgta cgactggctt gtaccgtagt
     1741 atataacgga aacaggaaca tacaggtgca tccgggtcag agcacctgtg tgattctgct
     1801 gatatccgga acagcgacaa gggagtaatt cgtatagtaa acgacaaatc ccggtttgct
     1861 gccattcggt tttttgatat tttttttctg gacatagtcg atctcgactt ttccctgttc
     1921 ccttcctttg gaatagtagg cggcgagact tcctgcttct tcaaatgttt tgtccggcag
     1981 gtcttccccg tttgacttta caataacatg agaaccaggg attccttttg catggaacca
     2041 ccagtcattt ccgacagcga atttaaaagt aagatcatca ttctgataat tgttttttcc
     2101 gacatagatc tgatagccgt cactgcttat ataatgaagc ggccggcttg tgaatttctt
     2161 ttttttcatg gtggattttc tgcggatata accgctctcg accagttcat ccttgatttc
     2221 ggccaggtct tcttcataca gggcaatatc cagggcagta ccaatagact ccagatgctc
     2281 aatttctttt tttgtttctg cagactgtat gtttacagct tcttcagttc gcttcagctt
     2341 attatattta tcaaaatatt tttttgcatt ttcctgcgga gaaagtgttt cattaagagg
     2401 gatggtaatt gtttccccgg tataataatt gtctgcttca aaggacttac agccggtttt
     2461 taatccgtag ccgtacgtgt tgagcagttc accgtatatt ctgaacttgt cttttttctg
     2521 cgtatcattc aattgtttga gctgcagctc atattttttt gtgttgcgtt caagagccgt
     2581 ctgtacgatt ctgcgaagat ccgcggattt ttggcggata cgagtgatgg tatttttttt
     2641 ggcgtaataa gtttcaagaa tataggagat cgaaggattt tcaagtgtct tcatatcgcc
     2701 gtactgcgtc aggggcagag cggcaaattc aaggggattg tcctccaaaa gaaaaataca
     2761 gggcttgaat cttttttcag aaacctcttc catcagccag ctgaaatgat tgtacagatg
     2821 gagcagatgt tcatcggaaa gtgtgttcgc agggagggag gagtcaatgg aagcgcggaa
     2881 taaaagctcc tctgccatta cgggagaaaa tcctgtcagc gttgtataga cggcttttgc
     2941 cgccggcatc ggtttggctg caatctgcga caggaacgtt tcttttgaaa tttcaaacgg
     3001 atcatatttt ttctgtgtca tcggaataaa ataatcccgt cccggaagca cttcccggac
     3061 agagctgaca gcgccggaaa tatgcttgat gctgtcgatg accgtatgat ggtcgtctaa
     3121 aaatatgata ttgctgtgtt ttcccatgat ctccacaaca agcgttttat gacatacatc
     3181 attcatttca tccagatgtt cgatttcaaa cataatgatc cgttccagtc cgggctggcg
     3241 gatggagaca atacgtccgt tctgcagatg cttgcgcagc agcatacaaa aattcggcgc
     3301 agtcatcggg cttt
//
LOCUS       DAOSYE010000151         3310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20784_length_3310_cov_3.509370, whole genome shotgun sequence.
ACCESSION   DAOSYE010000151 DAOSYE010000000
VERSION     DAOSYE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20784_length_3310_cov_3.509370"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLU43_03595"
     CDS             complement(<1..463)
                     /locus_tag="PLU43_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27524.1"
                     /translation="MKSKIGKIILFAVCLSAILFGINQILKYKSNDGINQMRAFYTND
                     ENSIDVLFMGSSHSYSNVNTGVLYDDFGIAAYDLGGAEQLLWNDYYWLVEALKTQKPK
                     VIVLEIFSTGMIKDDFQGAWIMENLYGMKLSKNYYESVKKSTLDTAFDSYIL"
     gene            complement(476..1990)
                     /locus_tag="PLU43_03600"
     CDS             complement(476..1990)
                     /locus_tag="PLU43_03600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO27525.1"
                     /translation="MVFNSVDFLVFFPVVLLIYFFIPKRIRYIWLLIASYYFYTSWDV
                     KYVGCLLFSTLVTYFAGLLIGRYRHAEKYDTSVQGGQKETLLFIICLLLNFSMLFIFK
                     YTNFMLDNIADLAGLLKITFKEPDIELLLPVGISFFTFQSAGYLIDVYKGEINPEKNL
                     LKYALFVSFFPNILSGPIERAKNMLPQIENCKQMKLWNYERITEGAVYMLWGYFLKMV
                     IADRSALLVNTVYGNYRMYGSVALITATFFFAVQIYCDFASYSYLALGAAKILGFSIM
                     NNFNAPYFSKSIGEFWRRWHISLSSWLRDYLYIPLGGNRRGRFRKHLNTIIVFFVSGV
                     WHGANWGYVVWGLLHGVYIVIGDLLRPYKKRAANTFHIKTQSFSYRLLQTGITFLLVC
                     FSWIFFKTEQLSAAVEVIRRMFVKWDPWTIFDGTIYTWGLSEKDFRITVFTILLLLAA
                     DLIKKKKGGNVIGFLNQQNIWFRWGIYIFICCAIAVWGQYGAGHDPQAFLYFSF"
     gene            complement(2105..>3310)
                     /locus_tag="PLU43_03605"
     CDS             complement(2105..>3310)
                     /locus_tag="PLU43_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27526.1"
                     /translation="DLGGAEQPYWNSYYYLKEALKTQTPKVIVLDITTPGIRAVDFQP
                     ENWVVCNIYGMHWNKNKYDALKVSTLEQSFERLAIPLNTIHGRYDELTKEDFTDTNQS
                     ISYKGFDPRETVTLFETPDISDVTKTAPISEKAEKYLRKIIELTKEENIPLLMISAPY
                     VVTEDAQMLYNYVFDIADESDIAYIDFNKPYEEMDLDFQTDMAENLHLNESGNEKFSK
                     YLGECLLEKYEIPDRCGNSAYISWETDAINQRQEEAKTSLENTGSISEYLPMLLNKNY
                     IVYLSFGDEMEDLYLDEEVRLQLNTLGISDEKLCGKSAVIVNNGNLLFQSDKEEFKAF
                     ISEGTDRLLYFREPDENDVYTTGIYINEDRYAMKKTGISILVYDRQLKQVVDHVNFNI
                     QNGFSLERQ"
BASE COUNT          994 a          776 c          554 g          986 t
ORIGIN      
        1 gaaggatata actgtcaaac gccgtatcca atgtgctttt tttcacagac tcataataat
       61 ttttcgacag cttcatccca tacagatttt ccatgatcca tgctccctga aaatcatctt
      121 ttatcatacc cgtcgaaaaa atctccagta cgatcacttt tggtttctgt gttttaagcg
      181 cctccacaag ccagtaataa tcattccaaa gcagctgctc cgccccgccc agatcatatg
      241 cggcaatgcc gaaatcatca tacagaacgc ctgtattgac attactgtag gaatgggagc
      301 tgcccatgaa caatacatct atggaattct cgtcatttgt ataaaatgcc cgcatttgat
      361 tgattccgtc atttgatttg tatttcaata tttgattgat cccaaataaa atagccgaaa
      421 gacacacagc aaatagaatt atttttccga ttttactttt cataaagccc tcgtttcaaa
      481 aactgaaata taaaaacgcc tgcgggtcat gccctgctcc atattgcccc catacggcaa
      541 ttgcacagca gataaaaata tagattcccc atcgaaacca aatattctgc tgatttaaaa
      601 atcctatgac atttccgcct ttctttttct ttatcagatc agcagccaga agcagcagga
      661 tcgtaaatac cgtaatacga aaatcttttt ccgaaagccc ccaggtgtaa attgttccgt
      721 caaaaatcgt ccacggatcc catttgacaa acatccgtct gatcacttca accgcagcac
      781 tcagctgctc tgttttaaaa aagatccagg aaaaacagac aagcagaaat gtaatccctg
      841 tctgcagcag ccgatagctg aaagactgcg tcttgatatg aaatgtgttt gccgctcttt
      901 ttttatacgg acgcagcaga tctccgatca caatatacac gccgtgcaaa aggccccaaa
      961 ccacatatcc ccagtttgcg ccatgccaga caccgctcac aaaaaatact atgatcgtat
     1021 tcaggtgttt ccgaaatcgg ccgcgcctgt ttcctccaag gggaatatat aaataatccc
     1081 gcagccagga gcttaaagaa atatgccatc tgcgccagaa ttcaccgata ctttttgaaa
     1141 aatacggtgc attaaaatta ttcataatgg aaaatccaag tatttttgcg gcgccaagcg
     1201 caagataaga atagctcgca aaatcacagt aaatctgtac tgcaaaaaaa aacgtagccg
     1261 taatcagtgc aacggaacca tacattcggt aattgccgta tacggtattt acaagcaatg
     1321 ccgacctgtc ggcaatcacc attttcaaga aataccccca cagcatataa acggcgcctt
     1381 ctgtgatccg ttcataattc cataatttca tctgtttgca gttttcgatc tgaggcagca
     1441 tgttttttgc acgctcgatc ggtcctgaca agatattcgg gaaaaaagaa acaaacaatg
     1501 cgtactttaa cagatttttc tccgggttta tctctccttt atacacatcg atcaaataac
     1561 cggccgattg gaatgtaaag aaagaaatgc cgaccggcag caataattct atatccggtt
     1621 ctttaaatgt aattttcaaa agcccggcca gatctgcaat attgtccagc ataaaattgg
     1681 tatatttaaa aataaagagc atggaaaaat ttaagagcag gcatatgatg aagagcagcg
     1741 tttctttctg tccgccctgt acggaagtat catatttttc cgcatgccgg tatcgcccga
     1801 tcagcaatcc ggcaaaatat gttaccaatg tggaaaacaa cagacaaccg acatatttca
     1861 cgtcccagct cgtgtaaaaa taatagctgg caataagcag ccaaatataa cggattcgtt
     1921 ttggaataaa aaaatatatg agcaatacta ccggaaaaaa tacgagaaaa tctacggaat
     1981 taaataccat atggtctcct gttatatgtt caactgtgcc tctttaaaat tcaatgcggc
     2041 tattatctgc taattcatta taacctgaaa agaataccgg cttcaatccc cagaatgtga
     2101 acttttattg tctttcaaga gaaaatccat tttgaatatt aaaattcaca tgatccacga
     2161 cctgttttaa ctgtctgtca taaacaagaa tgctgattcc ggttttcttc atggcatatc
     2221 tgtcttcatt aatatagatc ccggtcgtat aaacatcgtt ttcatccggc tctctaaaat
     2281 acagcagcct gtccgttcct tcgctgataa acgctttaaa ttcctcttta tcggattgaa
     2341 acagcagatt cccattattt acaataaccg cgcttttacc gcataatttt tcatcagata
     2401 ttccgagggt attcagctgc agtctgactt cttcatccag atacagatct tccatttcat
     2461 ctccaaaaga aagatatact atataatttt tatttaaaag catcggaaga tattcgctga
     2521 tactgcctgt attttccagg ctggtttttg cttcctcctg acgctggttt atggcatccg
     2581 tctcccatga aatatacgca ctgtttccgc acctgtccgg aatctcatac ttttcaagca
     2641 gacattctcc cagatatttg ctgaattttt catttccgct ttcatttaaa tgaagatttt
     2701 ccgccatatc cgtctgaaaa tcaaggtcca tttcctcata cggtttattg aaatcaatat
     2761 atgcaatgtc cgattcatcc gctatatcaa aaacataatt gtaaagcatc tgcgcatctt
     2821 ctgttacaac atacggcgct gaaatcatca ataacgggat attttcttct tttgtaagtt
     2881 cgatgatctt ccttaaatat ttttcagcct tttccgatat tggtgccgtc tttgtaacat
     2941 cggaaatatc cggcgtttca aacagcgtga ccgtttcccg cggatcaaac cctttatagg
     3001 agatcgactg attcgtatcc gtaaagtcct cttttgtcaa ttcatcgtag cgtccatgta
     3061 tggtatttaa cggaatcgcc agcctttcaa aagactgctc aagcgtactg actttcagtg
     3121 catcatattt atttttattc cagtgcatgc cgtatatatt acagaccacc cagttttccg
     3181 gctgaaaatc cacggcgcgg atcccaggcg tcgtaatatc caatacaatc actttgggtg
     3241 tctgtgtctt taacgcttct tttaaatagt aataactgtt ccagtacggc tgttccgctc
     3301 cgcccagatc
//
LOCUS       DAOSYE010000152         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20890_length_3300_cov_3.220955, whole genome shotgun sequence.
ACCESSION   DAOSYE010000152 DAOSYE010000000
VERSION     DAOSYE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20890_length_3300_cov_3.220955"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLU43_03610"
     CDS             <1..701
                     /locus_tag="PLU43_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOO27527.1"
                     /translation="FGELFHVNEADANLAHLPEGIGLAEAAMLSDMVPTGFHGVELAD
                     VQFGDSVVVIGIGPVGLMAVAGTALRGASHIYAVGSRPNCIQIAKEYGATDIVNYREG
                     DIVEQIMDKTDGKGVDKIVIAGGDVNTIDQAIRMLKPGGRIGNVNYLGEGDYVKVPRV
                     EWGCGMGHKIIAGGLMPGGRLRMEKLASLIETHRLDTGKLITHKFEGLESVEEALMLM
                     KDKPRDLIKPVVIL"
     gene            complement(744..1442)
                     /gene="deoC"
                     /locus_tag="PLU43_03615"
     CDS             complement(744..1442)
                     /gene="deoC"
                     /locus_tag="PLU43_03615"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOO27528.1"
                     /translation="MTTELLLKDLNRHIDISCVKGSHTRADIDSMLQYAKKYQFIGVF
                     AMPCYTSYIIEQLNGEPFIKPGGVVGFPAGCDTTRSKTEQAKELVHMGCQELDMVMAY
                     GMLKSGAYDYVLEDIKRVVSAGDGLPVKVIIEAPCLNDYEIAKACELSVRAGAAFVKS
                     GTGWAKTPSTVHMIRLMKDTVGERAFIKAAGGIRSLSVIMEMRELGCERFGMGVNSAV
                     SVMKELEAYRQKQT"
     gene            complement(1432..2175)
                     /locus_tag="PLU43_03620"
     CDS             complement(1432..2175)
                     /locus_tag="PLU43_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO27529.1"
                     /translation="MSNKLPSYLQVYHQLKQDILHGKYKVGEMLPVESDLEKIFSVSR
                     ITIRKAVKLLNEDKLVNVKQGRGTMVLDFKASQNLNHVISVTETFRKKGYTVTTKSMY
                     IDIIQADTELAEHLKIPAGENIARFQRIQLADGKPVVIMKNYVPEQLVPDVKKYCNKF
                     SALYQFLEDTYHIQIDAASDRIFAKNASFEEAQLLDIKTGDALLCIQRTCYEQDVPVC
                     LDDVAIIGRQYELTSNMQGRDRGTKKNDN"
     gene            complement(2230..3012)
                     /locus_tag="PLU43_03625"
     CDS             complement(2230..3012)
                     /locus_tag="PLU43_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N(6)-methyltransferase family
                     protein"
                     /protein_id="HOO27530.1"
                     /translation="MPPNYQPIKSLYFSQNFLTSTKTIERLLRISNIGKHDHVIEIGA
                     GKGHITRILVRKSKLVTAVEPDRQLFSKLQKNLKTIPNLKLLNIDFLKMRLPINESYK
                     VFSNIPFAITTDIMRKLFEAKTPPVETWLVMEKGAAKRFMGQPNENINSFYLKPFFDL
                     KIVYYFLKNDFHPKPSADTVLLYIKQKEHPDLALSERKNFKFFLTHISKYGMFNVLTK
                     KQVATALKLENLPPIEHADKIIDTQWLALFRYWSKFSSHHLT"
     gene            complement(3149..>3300)
                     /locus_tag="PLU43_03630"
     CDS             complement(3149..>3300)
                     /locus_tag="PLU43_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HOO27531.1"
                     /translation="TNTKSELELRNAAIYEDYKAGMSVKQLAEKYFLVEKSIQRIINK
                     QKSKK"
BASE COUNT          869 a          650 c          740 g         1041 t
ORIGIN      
        1 tattcggaga actttttcac gtaaacgagg cggatgcaaa tctcgcacat ttaccggaag
       61 ggatcggact tgcggaagca gccatgcttt ccgatatggt gccgaccggt tttcatgggg
      121 tagagcttgc cgacgtacag ttcggtgatt ctgttgtagt gattggaatt ggccctgttg
      181 gtcttatggc agtggcaggt acggctctgc gcggcgcatc ccatatctat gcggtaggtt
      241 ccaggccaaa ctgtattcag atagcaaaag aatacggagc aacagatatc gtaaattacc
      301 gtgagggcga tattgttgaa cagattatgg ataagacaga cggaaagggt gtagataaga
      361 tcgtgatcgc cggcggagat gtcaatacga tagatcaggc tatccggatg ttaaagccgg
      421 gcggaaggat cggaaatgta aattatcttg gagaaggtga ttatgtaaag gttccgcgtg
      481 tggagtgggg ctgcggaatg ggacacaaaa taatcgccgg cggattgatg cccggcggaa
      541 gacttcgtat ggagaagctt gcgtccctga ttgaaacaca ccggctcgat accggtaaat
      601 tgatcactca taaatttgag ggtcttgaga gtgtggaaga ggctctgatg cttatgaaag
      661 ataagccgag agatctgatc aagccggtag ttattttata aacacaggat cggacctgac
      721 agcggaaagg agatctgccg ctgtcaggtt tgtttttgcc tgtaagcttc cagttccttc
      781 atgacagaaa cagcagagtt aacgcccatt ccgaaccgct cgcatccaag ttccctcatt
      841 tccatgataa cagataagga gcggatgcct ccggctgctt taataaaagc tctttccccg
      901 actgtatctt tcataagccg gatcatatgc acagtagaag gggtttttgc ccatccggtt
      961 ccgcttttaa cgaaggcagc gccggcacgg acggataact cgcacgcctt tgctatttca
     1021 tagtcgttta ggcagggagc ttcaatgatc acctttacgg ggagaccgtc accggcggat
     1081 acgacacgtt tgatatcttc caatacgtaa tcataagcgc ctgattttaa cattccataa
     1141 gccattacca tatcaagctc ctgacatccc atatggacca gctcttttgc ctgctcagtt
     1201 ttggagcggg tagtatcgca gcctgcagga aagccgacaa cgccgccggg ctttataaac
     1261 ggttcaccgt ttaactgctc aatgatataa gaagtgtagc agggcatggc aaaaacgccg
     1321 ataaactgat atttttttgc atactgcagc atgctgtcga tatcggccct tgtatgactg
     1381 ccttttacgc agctgatatc aatatgacga ttcagatctt ttaacagtag ttcagttgtc
     1441 attttttttc gtaccccgat cccttccctg catgtttgaa gtgagttcat attgtctgcc
     1501 gataatggcc acatcatcca gacaaaccgg aacatcctgc tcatagcatg tccgctggat
     1561 gcaaagcagc gcatcaccgg tcttaatatc cagaagctgt gcttcttcaa agcttgcgtt
     1621 ttttgcgaaa atgcggtcgg atgccgcatc gatctgaata tgataagtat cctctaaaaa
     1681 ctggtataaa gcagaaaatt tattgcagta ttttttaacg tccggaacaa gctgttcggg
     1741 cacatagttt ttcataatga caacaggttt tccgtcggcc agctgaatcc tctgaaagcg
     1801 tgcgatgttt tcccctgccg gtatttttaa gtgttcggcc agttcggtgt ctgcctgtat
     1861 gatgtcaata tacatgcttt tggtagttac ggtatatcct tttttgcgga aggtttccgt
     1921 gacggatatg acatgattca ggttctggga agctttgaaa tccagtacca tagtaccgcg
     1981 gccctgcttt acattgacga gtttatcttc gttcagcagc ttgacggcct ttcggatcgt
     2041 aatgcggctt acactgaata ttttttccag atcactttca acaggcagca tttctccgac
     2101 tttgtatttg ccatgcagaa tatcctgttt taattgatgg tatacctgta aataagacgg
     2161 tagtttgttt gacatttaca cccctccggt tccaaactct ttgggggtaa ttatacatcc
     2221 tggcttattt tacgtcaaat gatgggatga aaattttgac cagtatcgaa aaagagcaag
     2281 ccattgtgta tctatgattt tgtctgcgtg ttcaattggc ggaagatttt caagtttcaa
     2341 tgctgttgcg acctgttttt ttgttaatac gttaaacatt ccatacttag aaatatgtgt
     2401 tagaaagaac ttaaagtttt tccgttcaga taaagctaaa tcgggatgct ctttttgttt
     2461 tatataaagc agaacagtat ctgctgatgg cttaggatga aaatcatttt taagaaaata
     2521 atatacaatt ttcaagtcaa aaaatggttt taaatagaat gaatttatat tttcgtttgg
     2581 ttgtcccatg aatcgttttg cagcaccttt ttccataacg agccaggttt caacgggagg
     2641 ggtttttgct tcaaacagct ttctcatgat gtcggttgtt attgcaaatg gaatattgga
     2701 aaatacttta taggactcat ttatgggtaa tcgcattttt aagaaatcta tatttagcag
     2761 ttttaaattt gggattgttt ttaaattttt ctgaagtttt gaaaacaatt gtctgtcggg
     2821 ttctactgcc gttaccagtt tgctttttcg taccaggatg cgggtgatgt gccctttccc
     2881 tgcaccgatc tcaattacat gatcgtgctt gccaatattg gaaattcgca gcagccgttc
     2941 tattgttttt gtgcttgtta agaaattttg ggaaaaatat aatgatttaa ttggctgata
     3001 atttgggggc atactatttt ctccgcagct ttaatactaa aatagatagt tatgttttta
     3061 tttagtttag cattatgtgg cggaataata gggtacacgt tttttcttat aaaaaatgtg
     3121 ccatgacaag catgacacat ttcagaattc acttttttga tttttgttta tttataatac
     3181 gctgaatact cttttcaact aaaaaatact tttcagccaa ttgtttaact gacatacctg
     3241 ctttataatc ttcgtatata gctgcattac gaagttcaag ttctgactta gtgttcgtac
//
LOCUS       DAOSYE010000153         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20909_length_3298_cov_3.168671, whole genome shotgun sequence.
ACCESSION   DAOSYE010000153 DAOSYE010000000
VERSION     DAOSYE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20909_length_3298_cov_3.168671"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..657)
                     /locus_tag="PLU43_03635"
     CDS             complement(217..657)
                     /locus_tag="PLU43_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27532.1"
                     /translation="MMGIKDKLVKIMIGVAAVVLTAVPFGMSYQIALAAETMQEDAAV
                     SSPVQNYDLVVVQDEEVPLASAPEVNYGRTILWVVSLTVTAVLLLTYFLWYSVYKRRI
                     LFLIGRLPEAEAESIRRMVTIFHPIRAMQTEKEIENRIAAEYVK"
     gene            924..1625
                     /locus_tag="PLU43_03640"
     CDS             924..1625
                     /locus_tag="PLU43_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO27533.1"
                     /translation="MKIDYFKNYKFFKVIQLALLIIFGITFFYYLYFDPSLKNNVYTN
                     KNLLTICVFLWAFMVYSLITIIWDFHQLEKNIIDVHALNQATYLDNLTGIPNRYSCDL
                     IFDKYSSAGDIGNLGCALISISNLEEINQMLGRETGNKILQDFSHIFEKFGDTYGFVG
                     RNSGNEFLAVLEDCSPPLINEFLDDLQLELDKYNQSSDQIPILLSCHYILNTEAGVSE
                     FADLITLLYKEAKRG"
     gene            1631..2197
                     /locus_tag="PLU43_03645"
     CDS             1631..2197
                     /locus_tag="PLU43_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO27534.1"
                     /translation="MPDFNHTYIGSLVTRSQANDSDAFAELYALTYRHIYNYAGHYLR
                     DSFLAQDAVQETYISALKNIASIKDPSLFIAWLNQICFHICFDICKKNNQCYGSTDSE
                     LIEIIRDERIDHNPEDKFEKKAETESIQKAIRLLPFNEQQVIVMRYYNEMKLEEIASA
                     LQCSRSSVKRYLTHGRDRLEEMLRKEES"
     gene            2198..2701
                     /locus_tag="PLU43_03650"
     CDS             2198..2701
                     /locus_tag="PLU43_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27535.1"
                     /translation="MFFHKKYNMDINTANQTLQNVFAACNKKPNTIPFDKLVLRGLAQ
                     TTLVSICKWSAAVFLLLVLLSPLAFRNPDFSVSSKGFGQRIAIVDHHLYTSSFEMELS
                     GGNIDYTNIYAKKPDGTVVFPSSVSLNGKTMTVRIPYDGTSLNIYIPDTDGNIIQAVL
                     SKKQTGN"
     gene            2712..>3298
                     /locus_tag="PLU43_03655"
     CDS             2712..>3298
                     /locus_tag="PLU43_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO27536.1"
                     /translation="MFSFKRHHSCFISIIIVIVTSFFFTACGKSSDSLEVKVTSAASA
                     EETRSSKAVVLVPIPGDSALYGNDSVVIDASNASEGYIMVNYKGSNSKVKLQLTGSNQ
                     VTYTYNLKNGYICLPLTADSGSYQVTVFENINGDQYATAYSDQIDITIKNKLGPYLYP
                     NQYVNFTKDSKAVKQGEKLAENATCDLDVVAAVYG"
BASE COUNT          975 a          724 c          603 g          996 t
ORIGIN      
        1 tcctaactaa ctggttaaat gttggcattc ttttcacctc ctgtaatata tgatcgtagt
       61 tccctgcact tattataaat cggcaataaa tgcaaataat ccagtgtgca caaaaaagaa
      121 cgcattttga tgcacgctag attattatag gtgtttccat actccgtgtc aagaactttt
      181 catacaaaac ctatcgtcag ccgcaattac ttacacttat tttacatatt cggcggcaat
      241 tctgttttcg atctcttttt ctgtctgcat tgccctgatc ggatgaaaaa tcgtcaccat
      301 acgacgaatt gattcagcct ctgcctccgg caatcttcct attaaaaaaa ggatacgtct
      361 cttataaaca gagtaccata aaaaataagt aagcagaagt accgccgtca ctgtcagcga
      421 cacgacccat aaaatcgttc ttccataatt gacttccggc gcagaagcaa gcggaacttc
      481 ctcgtcctgc acgactacca gatcataatt ctgtacagga ctgcttactg ccgcatcctc
      541 ctgcatcgtc tcagcagcca gggcaatctg ataactcata ccaaacggca ctgctgtcaa
      601 tacgactgct gccactccta tcattatttt cactaatttg tcctttattc ccatcatatc
      661 aaccacctta atataaaatg catcttttat ttataatccg cattacttac ccattcaata
      721 aaaaagactg tcgtaattca taaaacggtc cattttatac atatttttta ttgtattttt
      781 tctccttttt gtttcttttt atttcattat agattataat agttatgtta atctgaagcc
      841 gaagcaggta cggcatcaga cattataaat gtatgacctt aaaactattt tacatgatat
      901 tgaaatgtgg gaggagattt atcatgaaaa ttgactattt caaaaactat aaatttttta
      961 aagttattca gcttgccctg cttattatat tcggcattac atttttttat tatctctatt
     1021 ttgatccttc tttaaaaaat aatgtttata caaacaaaaa cctgctgact atctgtgttt
     1081 ttctttgggc tttcatggtt tattccctga tcacgatcat ttgggatttc catcagctcg
     1141 aaaaaaacat catagacgtt catgccctga atcaggccac ttatcttgac aacctgacag
     1201 gaatcccaaa cagatacagc tgtgatctga tttttgataa atattcgagt gccggcgata
     1261 ttggcaatct cggatgcgct ttgatctcca tcagcaatct tgaagaaatc aaccaaatgc
     1321 ttgggcgcga aaccggtaat aaaattttac aggacttttc gcatattttt gaaaaattcg
     1381 gggatacata cggcttcgtc ggacggaaca gcggaaatga atttctggcg gttctcgaag
     1441 attgttctcc tcctctgatc aatgaatttc tcgacgatct gcagctggag cttgataaat
     1501 ataatcagtc aagtgatcag attccgatcc tgctttcctg tcattatata ctgaatacgg
     1561 aagccggcgt ttctgaattt gccgatctga ttaccttatt atataaagaa gccaaacggg
     1621 ggtagcctgt atgcctgatt ttaaccatac ttatatcggc agtctcgtca cgcgctccca
     1681 ggctaacgac agcgatgctt ttgccgagct ttatgcattg acttaccggc atatatataa
     1741 ttatgccgga cattatctta gagattcttt ccttgcacag gatgccgtac aggaaaccta
     1801 tatttcagca ctgaaaaata ttgcaagcat taaagatcct tccttattta ttgcatggct
     1861 gaaccagata tgtttccata tctgttttga catctgtaaa aaaaacaacc aatgttacgg
     1921 aagcactgac tccgaattaa ttgagatcat acgggacgaa cgaattgatc ataatcctga
     1981 agacaaattt gaaaaaaaag cagagacaga atctattcaa aaagcaatcc gtctgcttcc
     2041 ctttaacgag cagcaggtta tcgttatgcg ttattataat gaaatgaagc tcgaggaaat
     2101 agcaagtgcc ctccaatgca gcagaagcag cgtcaagcgt tatctgacac acggccgtga
     2161 caggcttgaa gaaatgctta ggaaggagga atcgtaaatg ttttttcata aaaaatacaa
     2221 tatggacatc aatacggcca atcagacttt gcaaaatgtc tttgccgcat gtaacaaaaa
     2281 gccaaatacg atcccttttg acaagctggt actgcgcggt cttgcgcaaa cgactcttgt
     2341 ttccatctgt aaatggagcg ccgccgtatt tcttctgctt gttctgcttt caccgcttgc
     2401 tttccgcaat ccggatttct ccgtttcttc aaagggattc ggacaacgga tcgctattgt
     2461 tgatcaccat ctttatacct cctcatttga gatggaatta agcggcggca atatcgatta
     2521 tacgaatatt tatgcaaaaa aaccggacgg aactgtcgtg ttcccatctt cggtcagcct
     2581 gaacggcaaa accatgactg ttcggattcc ttacgacgga accagtctca atatttatat
     2641 tccggatacg gacggaaata tcatacaggc agttttatca aaaaaacaaa ccggcaacta
     2701 aggagaaaaa gatgttttct ttcaaacggc atcattcctg ctttatttct attattatag
     2761 tgattgttac ttcctttttt ttcacagcgt gcggcaaatc gtccgactcg ctggaagtca
     2821 aggttacatc tgccgcttcc gccgaagaaa cccggtccag caaagccgtt gtactggttc
     2881 cgataccggg cgattccgcc ctgtatggca acgattccgt tgttattgat gcgtccaatg
     2941 catctgaagg atatatcatg gtaaattata aaggcagcaa cagcaaggta aagcttcagc
     3001 ttaccggcag taatcaggtt acttatactt acaatctgaa aaacggatat atttgtctgc
     3061 cgctgactgc tgacagcggc agctatcagg tcactgtttt tgaaaatatc aacggtgatc
     3121 agtatgcaac ggcttattcc gaccagatcg atattacgat caaaaacaaa ctggggcctt
     3181 acctgtatcc aaaccagtat gtaaacttca caaaggattc aaaagcagtc aagcagggtg
     3241 aaaagcttgc tgaaaatgct acctgcgatc tggatgtcgt agccgcggta tatggata
//
LOCUS       DAOSYE010000154         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20922_length_3297_cov_2.336212, whole genome shotgun sequence.
ACCESSION   DAOSYE010000154 DAOSYE010000000
VERSION     DAOSYE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20922_length_3297_cov_2.336212"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLU43_03660"
     CDS             <1..1074
                     /locus_tag="PLU43_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27537.1"
                     /translation="TLSSGEKQKVAVASAAAMAPAVYVLDEPSANLDMAATKELAGIL
                     GQLKENGSTIVIAEHRLYYLTALADRIVYMTDGKIRTIYTAGQLRALTEWQLEKMGLR
                     ATKIERNGYGQCSKGAGREGSCPFVSIRDLCLRAGKNKEALLSAISFDIFPGEIAALT
                     GANGAGKTTLARTLCGLAKEAGGKILYERKEVKTSARFRQAWFVMQETDCQLFSESVL
                     GEMTVGRTESPALTKKAEKILKELDLWIYRERHPASLSGGQKQRLTLAVALMQETGLL
                     ILDEPTSGLDGRNLEKVVYCIKKQAAAGRAILVITHDHELVQKACTRMLYLKNGTLEK
                     DFLLKDKETFQAAVGCLLEKDSR"
     gene            1307..1750
                     /locus_tag="PLU43_03665"
     CDS             1307..1750
                     /locus_tag="PLU43_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO27538.1"
                     /translation="MGSLRITIEELQGEEEEEVIIKCRKMTPELLRIISRLKAQDALI
                     AYDGNEIHRVRPSDIFYIEAVDSRTFFYLKGRVYESKQKLYQLEEVLMGCDFLRISKS
                     VIVNLSRIRSLSPALSGRFEATLDNGEKVIISRQYVGDLKKCLGI"
     gene            1763..2194
                     /locus_tag="PLU43_03670"
     CDS             1763..2194
                     /locus_tag="PLU43_03670"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HOO27539.1"
                     /translation="MWLEEILKKMAISFFIIVTGVVGAIYIFCLIFSPDAVFSLTDIG
                     RIFLMAAAGDLPLMIFLSGTELTKKQMVLRKSIHFIVLSGILLYLAIRWDWVDPKSIK
                     EVAVFLLTVLLVYVVVFFVSGYRDKKLTYKLNERLREKYRS"
     gene            2347..3261
                     /locus_tag="PLU43_03675"
     CDS             2347..3261
                     /locus_tag="PLU43_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27540.1"
                     /translation="MEKVIEVERLRKNYKDLTAVKDISFYTEQGKLFSFLGPNGAGKS
                     TTISILCTFMDFDGGVCRIGGYDLVREAQEIRKIIGVVFQENVLDDLLTVRENLIVRA
                     GLYTSDKAMIHNAVYEVIRSAQLHEIIDRPYGKLSGGQRRRADIARALINRPKILFLD
                     EPTTGLDPQTRKSIWDTIKRLQTDTGMSVFLTTHYMEEAAESDYVMVIDHGELVAKGT
                     PTELKTQYSSDLLKLFASDRQSLQNFLHEKQLAYTLSSEAFLIKLPDTMAALPILETC
                     KAFISGFQVLQGTMDDAFLGITGKELRQ"
BASE COUNT          973 a          618 c          882 g          824 t
ORIGIN      
        1 acgctttcca gcggagagaa gcagaaggtc gccgtggcgt ctgctgccgc gatggctccg
       61 gccgtttatg ttctggatga accgtctgca aatctggata tggcagcgac aaaggagctg
      121 gcagggatcc ttggacagtt aaaagaaaac ggcagtacga tcgtgatagc cgagcaccgt
      181 ctttattatt tgacagcgct tgccgaccgt atcgtatata tgaccgatgg aaaaatacgg
      241 acgatctata cggcagggca gctgagagcg ttaacagaat ggcagcttga aaaaatggga
      301 cttcgtgcaa cgaaaataga acgaaacgga tacggacagt gcagtaaagg cgcaggcaga
      361 gaaggaagct gcccctttgt gagtatcagg gatctatgcc tgcgggcagg aaaaaacaaa
      421 gaagcgctgc tctctgcaat ctcctttgac atctttccgg gagagatcgc agccctgaca
      481 ggtgcgaacg gggccggaaa aacgacactt gccagaacgc tgtgcggact ggcaaaagaa
      541 gcggggggaa agattctgta tgagagaaaa gaagtaaaga cttccgcccg tttcaggcag
      601 gcgtggttcg ttatgcagga gacggactgt cagttgtttt ccgaaagtgt tttaggagaa
      661 atgaccgtcg gcagaacgga atcgccggca cttactaaaa aagcagaaaa aatactaaaa
      721 gagctggatc tatggatata ccgggaacgc catccggcgt ctttatccgg cggacagaaa
      781 cagcggctta cacttgccgt agcacttatg caggaaacag gactgttgat tttggacgaa
      841 ccgacatccg gtcttgacgg aaggaatctt gaaaaggtcg tttactgcat aaaaaagcag
      901 gctgcagcag gaagagcaat tctggtcatc acacatgacc atgagcttgt gcagaaggcc
      961 tgcacacgaa tgctctattt gaaaaacggc acgctggaaa aggattttct tttaaaggat
     1021 aaagaaacct ttcaagcagc agtcggctgt ttattggaaa aagacagcag gtaatacaga
     1081 tcaacttgcc atccggcgtc cggtatggga taataagtaa aacattaagc aggaaggcga
     1141 atccatatat ggaagtcaga aaagcagcat tgaaacttta taaaaagctg gaatacacga
     1201 aaacgggaga agtcctgttt agaaaaggat tgttttattt atttgacaaa aaactttgat
     1261 ggttcatcgc ttcttatcag ggagaacgat agggagaaga caggtggtgg gaagtttgag
     1321 gattacaata gaagagctgc agggagaaga agaggaagaa gtcattatca agtgcaggaa
     1381 aatgacgccg gagcttcttc ggatcatttc cagactcaaa gcgcaggatg cgctgatcgc
     1441 ctatgacgga aatgaaatac atcgtgtcag gccgtccgat attttttata ttgaagcagt
     1501 ggacagcaga accttttttt atctgaaggg gcgggtctat gagtccaaac aaaagctgta
     1561 tcagctggaa gaagttctta tgggctgcga ctttcttagg atctccaaat ccgtgatcgt
     1621 gaatttaagc aggatccgtt ctctgagtcc ggcattgagc gggcggtttg aagcaacttt
     1681 ggataatggt gaaaaggtga ttatttcccg tcagtatgta ggtgatctga agaaatgtct
     1741 tggtatttaa ggaggtgatg ttatgtggct ggaagaaata ttaaagaaaa tggcaatcag
     1801 tttttttatt attgtaacgg gagttgtagg tgctatttat atcttttgcc tgatcttcag
     1861 cccggatgca gttttttcac tgactgatat cggaaggatc tttttaatgg cagcagccgg
     1921 ggatctgcct ttaatgatat ttttgtcagg aacggaactg actaagaagc agatggttct
     1981 gcggaagagt attcatttta ttgtactttc cggaattctt ctgtatcttg cgatacggtg
     2041 ggactgggtc gatccaaaaa gcataaaaga ggtcgctgtt tttcttttga cggttctgct
     2101 tgtatatgtc gttgtatttt ttgtcagcgg atacagggat aaaaagctga catataagct
     2161 gaacgaacgg ctgagagaaa aatatcgttc ataacacaaa gcgggaagaa ctcttatacg
     2221 gagggtaagc gtgcagctta ccctccgtat ttttcaactt aacgggtaat atcggcagca
     2281 taccggtgta ccgattgaag aggacgggcc ggctcgctat aatgattcca aaaaggaggg
     2341 atcattatgg aaaaagtgat agaagtggaa agacttagaa agaattataa agatctgacc
     2401 gcagtaaaag atatcagttt ttataccgaa cagggaaaac tgttttcttt tcttggtccg
     2461 aacggagcag gaaaatcaac aacgatcagt attttgtgta cttttatgga tttcgatgga
     2521 ggcgtctgca ggatcggagg gtatgatctt gttcgggaag cacaggagat ccgaaaaata
     2581 atcggggtgg tctttcagga aaatgtactt gacgatctgc tgacggttcg tgaaaatctg
     2641 atagtgcgtg cgggtctgta cacatcggat aaagccatga tccataatgc tgtgtatgag
     2701 gttatccggt ctgcgcagct ccatgagatc attgaccggc cctacggaaa gctttccggc
     2761 ggacagcggc gcagggcaga cattgcaaga gctctgatca accggccgaa aatacttttt
     2821 ctcgatgaac cgacgaccgg tcttgatccg cagacaagaa aaagtatctg ggatacgatc
     2881 aaacgtctgc agacagatac aggcatgtcg gtttttctga cgacacacta tatggaagag
     2941 gcagccgaat ccgattatgt aatggtgatc gaccacggag aacttgtggc taaaggaacg
     3001 ccaactgagc tgaaaacaca gtattcatca gatctgctga agctgtttgc ttccgacaga
     3061 caaagcctgc aaaatttcct gcatgagaag cagcttgcgt acacgctttc atcggaagcg
     3121 tttttgatta agcttccgga tacgatggcg gcgcttccga tcctggaaac ctgcaaagct
     3181 tttatcagtg gatttcaggt gttgcagggg acgatggatg atgccttttt aggtattact
     3241 ggaaaggagt taagacaatg aatattttta caaaacggaa tttgaagcta ttttttc
//
LOCUS       DAOSYE010000155         3293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_20957_length_3293_cov_2.956455, whole genome shotgun sequence.
ACCESSION   DAOSYE010000155 DAOSYE010000000
VERSION     DAOSYE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20957_length_3293_cov_2.956455"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLU43_03680"
     CDS             complement(<1..858)
                     /locus_tag="PLU43_03680"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOO27541.1"
                     /translation="MKKRCRWLQGILAILIVLCFTGCQNTAAETDNVFYMYYVNKSDT
                     KVVREAYTPENTETEKLIAECLSVLSSQPENVELKSPVNDNVSITGWTLQEGQLRIDF
                     DKNYYQIDSIAEVLRRAAIVRTLSQIDGIDGISFTVEEAPITDSKELPIGVMTADSFV
                     DNTGAEINSYERTDLRLYFADESGTKLISSIESIAYSSNISMEKLVTEQIIEGPASAG
                     AHPTVSPTTKILGVTIKDGICYLNLSADFLTISYPVSEEMVVYSFVNSLTELPNVNKV
                     QISIDGESDM"
     gene            complement(869..2338)
                     /locus_tag="PLU43_03685"
     CDS             complement(869..2338)
                     /locus_tag="PLU43_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO27542.1"
                     /translation="MSIRNGVSVIFSRVKQRIIKSRLVRSLRFRIFLIICLVGIIPGI
                     ILHTAILQNYEKRAVSVKTAEIQNQCKILANHLVTYNYLTDTTSEVVNSELEQISNLY
                     DGRVLIIDQDLKIVKDTYSISQDKTMISEDVVKCFQKENISKYDNTNHYIEIATPITD
                     RTSGEVLGVMLISVSTDSIVDSMAILGRKAIILEFAMAVLIFTLAYFLSLMLLKPFKR
                     IISSINEIKEGFAEEKIYVPEYIETEAISEAFNQMLVRMKAVDDSRNEFVSNVSHELK
                     TPITSIKVLADSLVSNEDVPIELYREFMADIVEEINREDKIISDLLALVRMDKTVADM
                     NIAQADINALVEQILKRLCPIARKRNIELVLESIRPVNAEIDEVKFTLAISNLVENAI
                     KYNVENGWVKVTVDADHQFFTVEIADSGIGIPKEAQGHIFERFYRVDKSHSREIGGTG
                     LGLAITRSAVLMHRGAIKVKSLEGEGTVFIVKIPRSYIA"
     gene            complement(2295..2993)
                     /locus_tag="PLU43_03690"
     CDS             complement(2295..2993)
                     /locus_tag="PLU43_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO27543.1"
                     /translation="MKKRVLVVDDEKLIVKGIRFSLEQDGMEVACAYDGEEAVALAKN
                     HAYDMILLDLMLPKLSGLEVCQQIRGFSKVPIIMLTAKGDDMDKILGLEYGADDYITK
                     PFNILEVKARIKAIMRRAAPEREDPISGDADNHIEISDIRLETEGRRFYIEDKEINLT
                     SKEFDVLELLARNPDKVYNREKLLHLIWGADYPGDVRTVDVHIRRLREKIEPNPSEPK
                     YVHTKWGVGYFFKG"
     gene            complement(3011..>3293)
                     /locus_tag="PLU43_03695"
     CDS             complement(3011..>3293)
                     /locus_tag="PLU43_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOO27544.1"
                     /translation="VPTGEEIENLSDNASMTTAVVQEEASASSLVNINTASRDELMTL
                     PGIGEAKAGNIIRYREEAGSFREIEDIMLVEGIKTGLFNKIKEYICTGL"
BASE COUNT          851 a          842 c          577 g         1023 t
ORIGIN      
        1 catatcactc tctccgtcga tagagatctg taccttattg acgttcggca gctccgtaag
       61 ggaattcaca aaggaataca caaccatttc ctccgatacc ggataactga ttgtcagaaa
      121 atcagcactc agattcagat aacagatgcc atccttgatg gtcacaccca gtatcttcgt
      181 ggtcggtgaa acggtcggat gggctcctgc ggaagcaggt ccttctatga tttgctccgt
      241 taccagcttc tccatggaaa tattactgct gtaggcaata gattctatcg acgatatcag
      301 cttcgttccg ctttcgtccg caaaatacag tcgtaaatct gtccgctcat aggaattgat
      361 ttcagccccc gtattatcaa caaacgaatc tgctgtcata acgccgatcg gtagctcctt
      421 cgaatctgta atgggtgcct cctccacagt aaaggatatt ccgtcaatgc catctatctg
      481 cgataatgtc cgtacgatcg ccgctcgtct taaaacttct gctattgaat ctatctgata
      541 ataattttta tcaaaatcga tcctgagctg tccttcctgc agtgtccatc ctgttatgga
      601 aacattgtca ttaacaggac tttttaattc cacattttca ggctgtgaag acagaacgga
      661 aagacattcg gcaatcagtt tttccgtttc cgtattttcc ggcgtatacg cttccctgac
      721 tacctttgta tcgctcttgt taacataata catatagaat acattatctg tttcagccgc
      781 cgtattctga cagccggtaa aacacagaac tatgaggatt gccagaatcc cttgcagcca
      841 tctgcatctt tttttcatag attcctccct aagcaatata actgcgcggt atctttacga
      901 taaagactgt tccttcgcct tccaggcttt tcactttgat cgcacctctg tgcatcagca
      961 ctgcgcttct tgtaatcgca agtcccagac ctgttccgcc tatttctctg gaatgagact
     1021 tatctaccct gtaaaacctt tcaaatatat gtccctgtgc ttcctttggt attcctattc
     1081 cggaatcagc aatttcaacc gtgaaaaatt gatggtccgc atctaccgtc acttttaccc
     1141 agccgttctc aacattatac ttaatcgcat tctctacaag attcgaaatg gcaagcgtaa
     1201 atttcacctc gtctatttcc gcattgaccg gacgaatact ttcaagtaca agttctatat
     1261 tccttttcct ggcaatcgga cacagacgtt ttaaaatctg ttcgaccagc gcattaatat
     1321 ctgcctgggc aatgttcata tccgctacgg tcttatccat tctgacaagg gccagcagat
     1381 cactgataat tttatcctct ctgttgatct cttctacgat atcagccata aattcacgat
     1441 acagctctat tggaacatcc tcgttggata caagggagtc cgcaagaacc ttgatggatg
     1501 tgatcggcgt ttttaattca tgcgacacat tggatacaaa ttcgtttctg gaatcatcca
     1561 ctgctttcat acgaacaagc atctgattaa acgcttctga aatcgcctct gtttcaatat
     1621 actccggcac atagatcttt tcctcagcaa atccttcttt tatttcattg atcgaactga
     1681 tgattctttt aaagggtttc agcagcatca gggaaagaaa atatgccagt gtgaaaataa
     1741 gaactgccat ggcaaattca aggatgatcg cctttcttcc gagaatagcc atactgtcca
     1801 caatactgtc cgtcgaaacg ctgatcagca tgacaccaag cacttccccg ctcgtgcggt
     1861 ctgtgatcgg cgttgctatt tcaatataat gatttgtatt gtcatatttc gaaatatttt
     1921 ccttttgaaa gcattttacg acatcctcgg aaatcatcgt tttatcctgg ctgatggaat
     1981 aggtatcttt tacgattttc aaatcctgat caataataag gacccttccg tcatataaat
     2041 ttgaaatctg ttccagttcg ctgttcacca cttctgaagt cgtatcagtc agataattat
     2101 atgtaacaag atggttcgcc agaattttgc actgattctg tatctctgca gtttttacgg
     2161 atactgcccg tttttcataa ttttgcaata ttgcggtatg caaaatgatg cccggtatga
     2221 tccccaccaa acatataatc agaaaaatgc gaaaccgcag gcttcgcact agtcttgatt
     2281 ttataattct ttgtttaacc cttgaaaaaa taaccgacac cccatttcgt atggacatat
     2341 ttcggttcac tcggatttgg ttctatcttc tcacgcaggc gtctgatatg tacgtcaaca
     2401 gttctgacgt ctcccggata atcggccccc catatcagat gcaggagctt ttctctgtta
     2461 tacactttat ccggattgcg cgcaagcaat tccaggacat caaattcttt tgacgtaaga
     2521 ttgatttcct tatcctctat ataaaaacgt cttccctctg tttcaagacg tatgtcactg
     2581 atctcaatat gattatctgc atcaccggat attggatctt ccctctccgg tgcagccctg
     2641 cgcatgatcg ccttgatcct cgcctttact tccagaatat taaaaggctt cgtaatataa
     2701 tcatccgcac catattccag accaaggatc ttatccatat catctccctt ggcagtcagc
     2761 ataatgatcg gcactttgga aaatcctctg atctgctgac agacttcaag tcctgaaagc
     2821 tttggcagca tcagatccag cagaatcatg tcatatgcat gattctttgc aagcgcaacc
     2881 gcctcctcgc cgtcataggc gcacgctacc tccatcccat cctgctccag actgaaacgg
     2941 atccccttta cgatcagttt ttcatcatca actaccaata cccttttttt cattataatc
     3001 ctttccgaac tcaaagaccg gtgcagatat attccttaat tttattaaaa agtcctgttt
     3061 tgattccctc aaccagcatg atgtcctcga tctcccgaaa gctgcccgct tcttcacgat
     3121 agcggatgat attcccggcc tttgcctcac cgataccggg cagggtcatc aattcatctc
     3181 tggaagctgt gttaatattg acaagagaac ttgcagaagc ctcctcctgc accactgccg
     3241 ttgtcatgga cgcattatcc gataaattct cgatttcctc tccggtcggc aca
//
LOCUS       DAOSYE010000156         3289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21001_length_3289_cov_3.728819, whole genome shotgun sequence.
ACCESSION   DAOSYE010000156 DAOSYE010000000
VERSION     DAOSYE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21001_length_3289_cov_3.728819"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLU43_03700"
     CDS             <1..1086
                     /locus_tag="PLU43_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HOO27545.1"
                     /translation="GKPELARSNRNFEVYYVNQRYIKSSLISKALDEAYKPYLMLHKY
                     PLVVLYLTIPFHMLDINVHPSKMELRFLESEKIYRLLIAYIQEALSEKELIPEIKEET
                     AAAKSRPAPEEIFPEPFEINRREEFLKSTPVVQAKEPAPFVAETPVYHTVKPEQLNLF
                     EEKFLTENSRKSHRIIGQVFDTYWLIEMEDKLYIIDQHAAHEKVKYERLLKQVRENAV
                     ISQFLNPPSIFSLSKTEEQLLLRFLPNFEKLSFEIEHFGGSEYSLRAVPTELFQLSVE
                     DYFHELLDELSQEKLSETLPSINDRLATMACKSAVKGNNRLSGQEAQTLIDELLTLDN
                     PYHCPHGRPTIISYSKYELEKKFKRIV"
     gene            1091..2053
                     /gene="miaA"
                     /locus_tag="PLU43_03705"
                     /pseudo
     CDS             1091..2053
                     /gene="miaA"
                     /locus_tag="PLU43_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
     gene            2080..>3289
                     /locus_tag="PLU43_03710"
     CDS             2080..>3289
                     /locus_tag="PLU43_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOO27546.1"
                     /translation="MNKKTETIYRELGIDEEIYSYGEEILSGLRERFEAFDQTMEYNQ
                     LKVIRAMQACRVSEACFQATSGYGYNDLGRDTLEKVYAALFHTEDALVRPQITCGTHA
                     LAIALSANLRPGDELLSPVGKPYDTLEEVIGIRPSKGSLAEYQISYRQVDLLENGDFD
                     YENIKKAINENTKLVTIQRSKGYQTRPTLSVDKIGGLIAFIKQIKPDVICMVDNCYGE
                     FVEKIEPSEVGADMIVGSLIKNPGGGLAPIGGYIAGKKEYVENAAFRLTSPGLGKEVG
                     ASLGIIHSFYQGLFLAPTVVNGALKGAVFAASLYESLGYPVIPESTESRHDIIQAVTL
                     GSPEAVIAFCKGIQAAAPVDSFVTPEPWAMPGYDSDVIMAAGAFVSGSSIELSADAPI
                     KPPIRAWDELL"
BASE COUNT          996 a          677 c          798 g          818 t
ORIGIN      
        1 ggaaaaccag agcttgcgag aagcaatcgt aattttgaag tctattatgt caatcagcgt
       61 tatataaaaa gctccttaat atcaaaagcg cttgatgaag cctataagcc ttatttaatg
      121 cttcataaat atccgcttgt cgtactgtat ctgacaattc cgttccatat gcttgatatc
      181 aatgtgcatc cgtcaaaaat ggaacttcgt tttctggaat cggaaaaaat ataccgcctt
      241 ctgattgcat atatacagga agcgctgtct gaaaaagaac tgattcccga aataaaggag
      301 gaaacagcag ctgcaaaaag cagaccggcc cctgaagaaa tattccctga gccgtttgaa
      361 ataaacaggc gggaagaatt tctgaagtcc acacctgtag ttcaggctaa agaaccggcc
      421 ccatttgttg cggaaactcc cgtttatcat acggtaaaac cggaacagct gaatctgttc
      481 gaagaaaaat ttctgactga aaattccaga aaaagccacc ggatcatagg acaggtcttt
      541 gatacttact ggcttataga aatggaagat aaactatata ttatcgatca gcatgcggct
      601 catgaaaaag taaaatatga acggctgctg aagcaggtcc gggaaaatgc ggtgatctcg
      661 caatttctga atccgccgtc aattttttca ctttccaaaa cagaagaaca gcttctgctg
      721 cgtttcctgc cgaattttga aaagctcagt tttgaaattg aacatttcgg aggcagtgaa
      781 tattctctgc gtgctgttcc gacggaattg tttcagctgt ctgtggaaga ctattttcat
      841 gaactgttag acgaactttc acaggaaaaa ttgtcggaaa cactgccgtc cataaacgac
      901 cggcttgcga caatggcatg taaaagcgcg gtaaaaggca ataaccgtct ttccggacag
      961 gaagcacaga ccctgatcga tgagctgctg acactggata atccgtatca ctgcccgcat
     1021 ggacggccga cgatcatttc ttattcgaag tatgaactgg agaaaaaatt taaaaggatc
     1081 gtataaacaa atgaatgagc attgtgaaaa aagaccgctt attattttga ccggtccgac
     1141 agcagtcgga aaaaccggtc tttctattca gctggcaaaa aaaataaatg cggaaatcat
     1201 ctcagcagat tccatgcaga tctatcggta tatggatatt ggatctgcaa aaataaaacc
     1261 cgaagaaatg ggcggtatcc gtcattttct gatcaatgaa ttaagtcccg gggaagcatt
     1321 caatgttgtt gtgtttcaaa aaaaagcaaa ggcagcaata caggagattt acaacgccgg
     1381 aaaactcccg ctgcttgtcg gcggaaccgg tttttatatc cagtccatac tctatgatat
     1441 tgactttgaa gcaacaaaag aagatcctgg ctaccgtgcc ggactgcttg accaggcaca
     1501 ggagaatgat ccgcactatt tgcatgagct gctgaaaaac tgtgatccga aagcggcgga
     1561 agagatccat ttcaataaca cgaagcggct tatacgcgca ctggaataca accatctgac
     1621 gggaacgtgt atttccgcac ataacgaaac ggaacgacaa aaaaaatcgc cttattcgta
     1681 ttgctatttt gtgttaaatg atgagagagc gctgctttat gaacagatcg acaggcgtgt
     1741 cgacctgatg atgcaggagg gtctgcttga cgaagtgaag cgtcttaagg aaatggggta
     1801 tgacagatca tttgtttcca tgcaggggct tggatataaa gagctgttag cctgtcttga
     1861 cgaagaatat tctcttgagg aggcaatacg tattataaaa cgggacacca ggcattttgc
     1921 atagaggcag ctgacctggt ttcggcggga aaaagatgtg atctggctga ataaaaacga
     1981 atatgaccac agtgataaaa aactgcttgc acagattctt agcagtataa aagcagccaa
     2041 tattattagt tgaaaatata agaaggaaga actgacggta tgaacaaaaa aacagaaaca
     2101 atttacagag aacttggcat tgatgaagag atctattctt acggggaaga aattctcagt
     2161 ggtctgcggg agcgttttga agcttttgat cagacaatgg aatataatca gctgaaagtc
     2221 atccgcgcca tgcaggcatg cagggtgtcg gaagcctgtt ttcaagccac gagcggttat
     2281 ggatacaatg accttggcag agatacgtta gagaaggtat atgcagcatt attccacacg
     2341 gaagacgctc ttgtcaggcc gcagatcacc tgcggcacgc atgcgcttgc aattgcgctg
     2401 tctgcaaacc tgcggcccgg agacgaactg ctttctccgg tagggaaacc atacgataca
     2461 ctggaagaag tgatcggcat ccgtccgtcc aagggttccc ttgccgaata tcagatatct
     2521 tacaggcagg tagatctgct tgaaaatggg gacttcgatt atgaaaatat caaaaaagca
     2581 atcaatgaaa atacaaaact tgtcactatc cagcgttcca aaggctatca gacgcgaccg
     2641 actctgtcgg tcgataaaat aggcggtctg attgccttta tcaaacagat caaaccggac
     2701 gtgatctgta tggttgataa ttgttatgga gaatttgtgg aaaaaatcga gccgtctgaa
     2761 gtcggcgcag atatgatcgt cgggtctctg atcaaaaatc ccggcggggg actggcaccg
     2821 atcggcggtt atattgctgg aaaaaaggaa tatgtggaaa atgccgcttt ccggctgact
     2881 tccccaggtc ttggaaagga agtgggtgca tcgctcggaa ttattcacag cttttatcag
     2941 ggtcttttcc tggcgccgac tgttgtgaac ggcgcattaa aaggtgctgt ttttgcagca
     3001 agtctttatg aatcgcttgg ctatccggtc attccagaga gtacggaaag ccggcacgat
     3061 attatccagg cagtgacgct tggatcaccg gaagctgtga tcgctttctg caagggaatt
     3121 caggcagctg cgcccgttga cagctttgtg acaccggaac catgggcaat gcccggttat
     3181 gacagtgatg tcatcatggc ggcgggtgcg tttgtgtccg gttcttcgat cgaattgagt
     3241 gcggatgctc cgatcaaacc tccgataaga gcatgggacg aactgctgg
//
LOCUS       DAOSYE010000157         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21120_length_3279_cov_3.197581, whole genome shotgun sequence.
ACCESSION   DAOSYE010000157 DAOSYE010000000
VERSION     DAOSYE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21120_length_3279_cov_3.197581"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PLU43_03715"
     CDS             complement(<1..154)
                     /locus_tag="PLU43_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOO27547.1"
                     /translation="MEVILAKKAGFCFGVERAVATVYEQAEKGEQIYTYGPIIHNEEV
                     VRDLEQG"
     gene            complement(182..853)
                     /gene="cmk"
                     /locus_tag="PLU43_03720"
     CDS             complement(182..853)
                     /gene="cmk"
                     /locus_tag="PLU43_03720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOO27548.1"
                     /translation="MNYNIAIDGPAGAGKSTVAKAIAKKKGFIYVDTGAMYRAMALYF
                     LREGIAAGETDKIEAACEKADISIDYADGVQIVLLNGENVNGLIRTEQVGNMASSSSV
                     NPKVREKLVALQQKLAAEKSVVMDGRDICTKVLTDAAVKIYLTADTKVRAKRRYDELV
                     EKGENCDIAAIEKDIQERDHRDMTRAVSPLVRAEDAVLVDSSNMPVEEVVRTILSISD
                     KKSGE"
     gene            complement(897..2126)
                     /locus_tag="PLU43_03725"
     CDS             complement(897..2126)
                     /locus_tag="PLU43_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO27549.1"
                     /translation="MKVIVAGGGPAGMMAAIAAAKCGYQVRLIEKNEKLGKKLFITGK
                     GRCNLTNACDMETVLQNIISNRKFLYSALYGFSNWQTMEFFEELGLKIKTERGGRVFP
                     VSDHSSDVIRVLEKELHSQNVAIHLRTKLTHVLVKDGHVTGIRLSDDTEIFADAVILA
                     LGGKSYPSCGADGDGWRIAKTLHMNTVEAEPSLVPLVVRESYAAKLQGLSLKNVRASV
                     YYGDIKLFEEFGEMLFTHFGVSGPLILSASSHIRESMYAQNLRLLIDLKPALDEKQLD
                     DRILRDFGENKNRQFRNSLGKLLPVKLIPVIIELSSVDGEKRVNEITKEERRKLVGLL
                     KALPMTIIGNRGFDEAIITRGGVSVKEINPATLEVKKIKGLYMAGEMLDIDALTGGFN
                     LQIAWSTGYAAGSSIGN"
     gene            complement(2136..2690)
                     /locus_tag="PLU43_03730"
     CDS             complement(2136..2690)
                     /locus_tag="PLU43_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO27550.1"
                     /translation="MARKEQITKQVILEGAFNLLREQGNQMVTARKLAAYIGCSTQPI
                     FRVYQNMEELNADIFLKAREYYEKYYLQYKKESDVPFVDLGLCYIQFAKEELNLFKLL
                     FLSAHDEENTMYDLINGSEQGFVIKELRRIPDLNMEHAGMIFMKIFIFIHGMACMATS
                     GEFDLTKEEVIPMLTDVVDTFRRS"
     gene            complement(2847..>3279)
                     /locus_tag="PLU43_03735"
     CDS             complement(2847..>3279)
                     /locus_tag="PLU43_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882851.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOO27551.1"
                     /translation="VLEEVPRVRKDLGEPPLVTPSSQIVGTQAVFNVLMGERYKMATK
                     ETKAVLRGEYGQTVKPMNPEIIEKVIPGEERITCRPADLIPNELEKLESEMKEWKQQD
                     EDVLTYALFPQVAIDFFKYRQAQQEKMDMTKSDSANGTYPV"
BASE COUNT          803 a          914 c          536 g         1026 t
ORIGIN      
        1 ccccttgttc cagatccctg acgacctctt cattgtgaat gatcggtccg tatgtataga
       61 tctgttctcc tttttccgcc tgctcataga cagttgcaac tgcccgctcc actccaaaac
      121 agaagccggc ctttttagcc agtattactt ccatgcctgt tccccttccg gacgtttccg
      181 cttattctcc tgacttttta tcactgatgg aaaggatcgt tcgaacgact tcttctaccg
      241 gcatattgga agaatccaca agcacggcat cttccgcccg cacaagaggt gaaaccgcac
      301 gtgtcatatc tctgtgatcg cgttcctgga tatccttttc gatcgcggca atgtcacagt
      361 tctctccttt ttccacaagt tcatcatatc gtctttttgc acgtactttt gtatcggctg
      421 ttaaatagat tttcaccgcc gcatccgtca gcacttttgt acaaatatcc ctgccgtcca
      481 taacaacact cttttctgcc gccagtttct gctgcagggc caccagtttt tcccgtactt
      541 ttggattgac ggaactgctt gatgccatat ttccaacctg ctctgtccgt atcagcccat
      601 ttacattttc gccgttcagc aaaacgatct gcaccccgtc agcataatca atggatatat
      661 ccgccttttc acaggctgct tcaatcttat cggtctcgcc tgcggcgatc ccttcccgca
      721 aaaaatacaa tgccatcgca cggtacattg ctccggtatc cacataaata aaccccttct
      781 ttttggcaat tgctttcgcg accgtgcttt ttccagcccc ggcaggtccg tctattgcaa
      841 tattataatt catccttctc ctccgtctca aaaaatatct gtttcatttt gcagaatcag
      901 ttaccgatac tgctgcctgc agcatatccg gtcgaccatg cgatctgcag attaaagccg
      961 cctgtcagcg catcaatatc cagcatctca cccgccatat acaagccttt gatttttttc
     1021 acttccagcg ttgcaggatt tatttccttt acgctgaccc ctcctcttgt aataatggct
     1081 tcgtcaaatc ctctgttgcc tatgatcgtc atcggtaacg ccttcagcag tccgacaagt
     1141 tttctgcgct cttcttttgt tatttcattt accctttttt ccccgtcaac agaagaaagc
     1201 tctataatca caggtatcag ctttaccggc agcaatttcc cgagcgaatt tctgaactgt
     1261 ctgtttttat tctccccaaa atccctaaga attctgtcat ccagctgctt ttcgtcaagc
     1321 gcaggcttca gatcgatcag aagtcgtaaa ttctgcgcat acatgctttc cctgatatgg
     1381 ctgcttgcgc tcaaaatcag agggccgctg acaccaaaat gcgtaaacag catttcccca
     1441 aactcttcaa atagttttat atcaccataa taaacagatg ccctgacatt ttttaacgaa
     1501 agtccctgca gcttcgccgc atagctttcc ctgactacaa gaggaaccaa cgatggttcg
     1561 gcttcaaccg tattcatatg aagtgtcttt gcaatgcgcc agccatcccc gtccgcgccg
     1621 cacgacggat aggatttccc gccgagcgcc agaataaccg catctgcaaa aatttccgta
     1681 tcatccgaca gccggatacc ggtcacatgt ccatccttaa caagaacatg cgtcagtttc
     1741 gttctcagat gaatagcaac attctgggaa tgcagttcct tttccagaac cctgattaca
     1801 tcagaagaat gatcggatac gggaaacacc ctgcctcccc gttcggtttt tattttcaga
     1861 cccagctctt caaaaaattc catcgtctgc cagtttgaaa aaccatatag tgcgctatat
     1921 aaaaattttc tgtttgatat gatattttga agcactgttt ccatatcaca ggcattcgtc
     1981 agattgcatc tccctttccc ggtaatgaaa agctttttcc caagcttttc atttttttcg
     2041 atcagcctga cctgatatcc gcattttgcc gccgcaattg ccgccatcat acctgccggt
     2101 ccgccgcctg ctactatcac tttcataaaa atacctcagc ttctgcgaaa cgtgtccacc
     2161 acatccgtca gcattggaat cacttcttcc tttgtcagat caaattcgcc gcttgttgcc
     2221 atgcatgcca taccatgaat aaaaataaaa attttcataa agatcattcc tgcatgctcc
     2281 atattcaaat caggaatccg tcttaattct tttatgacaa acccctgttc acttccatta
     2341 atcaaatcat acattgtgtt ttcttcatca tgtgcggaca aaaacaaaag tttgaacaaa
     2401 ttcaattctt cctttgcaaa ctgaatatag caaagaccca gatccacaaa cggaacatca
     2461 ctctcttttt tatactgcag ataatatttt tcataatatt ctctggcttt caggaaaata
     2521 tcggcgttca gttcctccat attctgatat accctgaaaa tcggctgcgt ggagcatccg
     2581 atataagctg ccagctttct tgccgtcacc atctgatttc cctgttctct caacagatta
     2641 aaagctccct ctaatatgac ctgttttgta atttgttctt ttctcgccat ccgcaatccc
     2701 tcctgactac caaccgacaa acaggcagtt cccttacaaa ctaccttgct tcggaaactg
     2761 cctgtttatt ataacatatg ttatgatatt ttgcactacc tttatacttt cattttaaaa
     2821 gattttatca caaattattt tgattcctat accggatacg ttccgtttgc agaatctgac
     2881 tttgtcatat ccatcttttc ctgctgtgcc tgacgatatt taaagaaatc aatagcaacc
     2941 tgcgggaaca gcgcataagt caatacatct tcgtcctgct gcttccattc cttcatttct
     3001 gattcaagct tctccaactc gttcggaatc agatctgcag gtctgcaggt gatcctttct
     3061 tctccaggaa taaccttttc gataatttcc ggattcatcg gcttcacagt ctgtccatac
     3121 tcaccgcgca gaactgcttt tgtttctttc gttgccatct tatatctttc acccatcaat
     3181 acgttaaata cggcctgtgt accaacgatc tgtgaagaag gcgtaacaag aggcggttcc
     3241 ccaagatctt tcctgactct tggtacttct tccagcaca
//
LOCUS       DAOSYE010000158         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21212_length_3268_cov_2.792095, whole genome shotgun sequence.
ACCESSION   DAOSYE010000158 DAOSYE010000000
VERSION     DAOSYE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21212_length_3268_cov_2.792095"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1698
                     /locus_tag="PLU43_03740"
     CDS             223..1698
                     /locus_tag="PLU43_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HOO27552.1"
                     /translation="MIDLTRNEEALEKNIKTARENNIIIPTFAQMKNPELIPEAIVEK
                     LKTVGLWDVNPLNLFRITWKNEQKEYGGGFHGSNYVVLPSELTGVKAKIVCLVGKWFP
                     TGCHKVGASFGCLVPRLVTGQFDTTYHKAVWPSTGNYCRGGAFNSKLLAAESIAILPA
                     EMSRERFDWLKSIAGEVIATPGCESNVKEIFDKTWELRKTRKDVLIFNQFDEMGNYLW
                     HYNVTGSAIADAFEEMKEDGDRFAGAVFTSGSSGTLASGDYLKEKYPSVKLGVGEALQ
                     CPTLLDNGFGGHRIEGIGDKHVPWIHNVKNTDMVIDIDDEDSQKLLRFFNEPVGREYL
                     VKECGVAPEFVELLGYFGISGIANMLCAIKMAKYYELTEHDVVATVMTDSADMYNSRI
                     REQQEETGEYTPTMAAVDYAAHIASIKTDYMEELTYLTKKRIHNLKYYTWIEQQGRKV
                     EELNALWYDEAGTWGSVRRQDKEIDALIDEFNERCGLIKNL"
     gene            1794..3026
                     /locus_tag="PLU43_03745"
     CDS             1794..3026
                     /locus_tag="PLU43_03745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO27553.1"
                     /translation="MKNVISAVCIKCGAEHEAVPGITTCEKCGGILDIKYDYRYIKSQ
                     LSPQKLTERKDNTMWRYREFLPVSGESKVPKLRVGWSPLYKADRLAEQLGLAELYIKD
                     DGLNPTASLKDRASAMAVVKAEEAGQKTIACSSTGNAASSLAGNAAAAGFRTFIFVPE
                     RAPKGKVAQLLMFGATVISVKGTYEDTFRLSAQAIEQWGWYNRNAAVNPYLMEGKKTV
                     SYEIAEQLHFKMPDYVAVSVGDGCTISGVWKGFRDLYEAGFIDRLPRLISVQAEGCCP
                     LNRAAAENKDWYPMEENTLADSIAVGVPRNPDKALHAIRESKGIVVNVSDEEILAAMK
                     LLGTTCGVFGEPAGVAGTAGVKKAVSQGLIPKSASVVSIVTGNGLKDVSNAMKAAGEP
                     IALEPDMKLLENALAGRL"
     gene            complement(3044..>3268)
                     /locus_tag="PLU43_03750"
     CDS             complement(3044..>3268)
                     /locus_tag="PLU43_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO27554.1"
                     /translation="NVNDLLDDLIKESVSMIGKPVALMSKDEKVNAIRFLNDAGAFLI
                     TKSGDKVSKYFGISKYTLYSYIDVNNNKSK"
BASE COUNT          940 a          631 c          909 g          788 t
ORIGIN      
        1 ttaattttct caaaatccat agtgttctcc tccttattat ttaagcttat catagcataa
       61 caggtataaa atgcaataga aaatacaaaa agttttagga aacaaaaaat attttgtttt
      121 ctgcattgat ttttatatat gagaatgcta cactgaaaat aataaaataa acaatgattt
      181 atttcaataa aatcatgaca tagagaacca ggaggagaca acatgatcga tctgacaaga
      241 aatgaagagg cattggaaaa aaacataaaa acagcaaggg agaacaatat tattattccg
      301 acattcgccc agatgaagaa tccggagctg ataccggagg cgatcgttga gaaattaaaa
      361 acggtcggac tgtgggatgt caatccgctc aatctgttcc gtattacatg gaagaatgag
      421 cagaaggagt atggcggcgg ctttcatgga agcaattatg tggtgctgcc ttccgagctt
      481 accggtgtga aggcaaaaat cgtctgtctg gtcggcaagt ggtttccgac cggctgccat
      541 aaagtgggcg cgtctttcgg atgtcttgta ccgcgtctgg taacgggaca gtttgacacg
      601 acgtatcata aggccgtatg gccgtcaaca ggcaactact gcaggggcgg cgcttttaat
      661 tcaaagctgc ttgcagccga gtcgatcgcg atcctgcctg ccgaaatgag ccgtgaacgc
      721 tttgactggc tgaaaagcat tgcaggtgag gtgatcgcca caccaggctg tgagagtaat
      781 gtaaaagaga tctttgataa aacatgggaa ctgcgaaaga caagaaaaga cgttttgatc
      841 ttcaatcagt ttgatgaaat gggtaattat ttatggcatt acaatgtgac gggaagcgcc
      901 attgcggatg cgtttgaaga aatgaaagag gacggggacc gttttgcagg cgccgtattc
      961 acatccggtt catccggtac gcttgcttcc ggcgattatc tgaaggaaaa gtatccaagt
     1021 gttaaactgg gcgtcggtga agcactgcag tgtccgaccc tgcttgacaa tggtttcgga
     1081 ggacaccgga tcgaagggat cggagacaag catgtgccgt ggatccataa tgtgaaaaat
     1141 acggatatgg tgatcgatat tgacgatgaa gacagccaga agctgcttcg tttctttaac
     1201 gaaccggtcg gaagggaata ccttgtaaaa gaatgcggtg ttgcaccgga gtttgtggag
     1261 ctgcttggat attttggcat ctcaggcatt gcaaatatgc tctgtgcgat caagatggca
     1321 aaatactatg agctgaccga gcatgatgtg gtcgctaccg ttatgacgga ttcggccgat
     1381 atgtataatt ccagaatcag agaacagcag gaagagaccg gggaatatac accgacgatg
     1441 gctgctgtcg attatgcggc acatattgcc tcgatcaaga ctgattacat ggaagagctg
     1501 acgtatctga caaagaagcg aatccataat ctgaaatatt atacatggat cgaacagcag
     1561 ggcagaaagg tagaggaact gaatgcactc tggtatgacg aagccggcac atggggttcc
     1621 gtgcgcagac aggataagga gatcgatgcg ctgatcgacg agttcaacga aagatgcggg
     1681 ctgataaaaa atttgtaaga gcagaaaata aaagcagaga atgcatttcc atggcgggaa
     1741 tgcattcttt ataaagaaag aaaacggatt tcggaggaaa ggctatggaa aaaatgaaaa
     1801 atgtgatctc ggctgtctgt atcaaatgcg gtgcggaaca tgaagcagtg ccgggtatta
     1861 cgacctgtga aaagtgcggc ggtattttgg atattaagta tgattaccgg tatattaaat
     1921 cgcagctttc accgcagaag ctgacagaac gaaaagacaa tacgatgtgg cgctaccggg
     1981 agttccttcc ggtaagcggg gaaagcaaag tgcccaagct tcgtgtcggc tggtcgcctc
     2041 tttataaggc ggacagactg gcagaacagc tgggtctggc agaattatat atcaaggacg
     2101 acggactcaa tccgactgcg agcctgaaag acagggcatc ggctatggca gttgtcaagg
     2161 cagaggaagc cggtcagaaa acgatcgcat gctcttcgac gggaaatgca gcctcttccc
     2221 ttgcgggaaa tgcggcggcg gcaggttttc ggacatttat tttcgttccg gaacgggctc
     2281 cgaaggggaa agttgcgcag cttttgatgt tcggggctac ggtgatcagt gtgaaaggga
     2341 cctatgaaga tacgttcaga ctttcggcac aggcgatcga acaatggggc tggtataacc
     2401 ggaatgcggc ggtcaatccg tatctgatgg aagggaaaaa aacggtctct tatgagatcg
     2461 cagaacagct tcattttaaa atgccggatt acgtagctgt ttcggtaggc gacggctgta
     2521 cgatcagcgg cgtctggaaa ggttttcggg atctgtatga agcgggattt atcgacaggc
     2581 tgccgcgtct gatcagtgta caggcagaag gctgctgtcc tttgaatcgt gccgctgcgg
     2641 aaaataaaga ctggtatccg atggaagaaa atacgcttgc ggacagtatc gcagtcggtg
     2701 ttccgagaaa tccggataaa gccctgcatg cgatcaggga atcaaagggg atcgttgtca
     2761 atgtgtccga tgaggagatc cttgcggcga tgaaactgct cgggacgaca tgcggtgtgt
     2821 tcggagagcc ggcaggggtt gcgggaacgg caggcgttaa aaaagcagtc agtcagggac
     2881 tgatccctaa atctgcttcc gtagtcagta tcgtcaccgg aaacggtctg aaggatgttt
     2941 ccaatgcgat gaaagcggcc ggtgagccca ttgcccttga accggatatg aagcttttgg
     3001 aaaacgcact tgccggcagg ctgtaggaat gcaggtgtct gcactatttt gatttgttgt
     3061 tatttacatc gatatagctg taaagtgtat atttggatat accaaaatat tttgatactt
     3121 tatctcctga ttttgtaatg aggaatgcac cggcatcatt gaggaaacgg atggcgttga
     3181 ccttttcatc cttgctcatc agagcgacgg gcttgccgat catggaaacg gattctttga
     3241 tcagatcatc gagcaggtcg ttgacatt
//
LOCUS       DAOSYE010000159         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21270_length_3262_cov_2.860617, whole genome shotgun sequence.
ACCESSION   DAOSYE010000159 DAOSYE010000000
VERSION     DAOSYE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21270_length_3262_cov_2.860617"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PLU43_03755"
     CDS             complement(<1..1496)
                     /locus_tag="PLU43_03755"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOO27555.1"
                     /translation="MSTKAYYPIAEIGAGKVGFSRTRSIIEAAFYGNNVVKVNTLKEA
                     YELAKNSPGTIVTDMKIKDGETFGLEKDSRVLLFNDGAVTGRYAFARRIKGEPGVDTV
                     KLDKVVMDAVYKSRWKTMYHAECFIGLDAEFMAKAHLLIPEGEENLLYNWMLNFQYMS
                     DEYVKLYKKSKPIADGNEPDIYIFSDPQWVPQPSPDVDYSCLNDPLTLCYFDTNQNCA
                     AILGMKYFGEHKKGTLTMGWALANRNGYASCHGGQKEYTLEDGSKFVASVYGLSGSGK
                     STLTHAKHNNKYPSIKVLHDDAFIINADTCASIALEPTYFDKTADYPTGCPDNEYLLS
                     AQNCSATLDSEGKVQLVTEDVRNGNGRAIKSKLWSPNRVDKIEAPVNAIFWIMKDPTI
                     PPVVKLKGSALASVMGATLATKTSSAERVAVGTDMNALRIVPYANPFRTYPLVNDYDK
                     FKKLVKEKNVDCYIVNTGDFMGKKVKPADTLGILETIVEKKDKFEQWGP"
     gene            complement(1609..>3262)
                     /locus_tag="PLU43_03760"
     CDS             complement(1609..>3262)
                     /locus_tag="PLU43_03760"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOO27556.1"
                     /translation="SGFTGSAGTLLVMADRAILWTDGRYYVQAEKELASTKIELYRMQ
                     EKGVPAIREFLRDVLAYGMCVGCDGRTISTSFYLELLKITEETGALIRSDIDLADRVW
                     PERPPMTKNGVYFLDLSIAGSDTGTKLAQLRRLLAKKDADAYVLTELGSIMWLFNLRG
                     SDIACNPVAFSYAYISQEQAILFLPEDGSEKETANVLLKFGVRIASYDEIEGFLLKLS
                     GERLLCDPQMVNCRLYQCMKEKNRVLFGENTEMIEKAVKNDIEISHARKYHVIDAVSV
                     LRFIIYIKEQVKTAHVTEYEAAKYLDALREKNEGFVGLSFETICAYKENAAIVHYSAK
                     PEGSALLHPKGMLLVDSGGQYLGATTDITRTIVLGELTMEEKEHYTAVLKGLLHLADA
                     RFLEGCTGENLDILAREPIWRLGIDYRHGTGHGIGSFLNVHEGPQAFRFKINREHLQP
                     PLQAGMITSDEPGIYIEGSHGIRLENELLCVKKEETEWGSFLGFETLTLVPFDLDAVL
                     PKLMSEKEKILLNVYHANVFRTLCPHLTDSEKTWLAAHTHKI"
BASE COUNT          864 a          835 c          599 g          964 t
ORIGIN      
        1 ggtccccact gctcaaactt gtctttcttc tcaacaatcg tctcaagaat accaagcgta
       61 tcggcaggtt ttaccttttt acccatgaaa tcacctgtat ttacaatgta gcagtccaca
      121 ttcttctctt taacaagctt cttgaattta tcataatcat tcacaagcgg atatgttctg
      181 aacggatttg cgtaaggtac gattctaagc gcattcatat ccgtacctac ggcaacacgc
      241 tctgcagaag aagtcttcgt agcaagtgtt gcacccataa ccgatgcaag agcagaacct
      301 ttcagcttta ctaccggcgg gatcgtagga tctttcatga tccagaagat cgcattaaca
      361 ggtgcttcga tcttatccac acggttcggt gaccaaagtt ttgacttgat ggcacgtccg
      421 ttaccatttc tgacatcctc agttacgagc tgaacctttc cttccgaatc aagcgttgcg
      481 gaacagttct gtgctgacag cagatattca ttatcaggac atcctgtcgg ataatcagcc
      541 gtcttatcaa aatatgtagg ctccaaagca atagaagcac atgtatctgc attaataata
      601 aatgcatcgt catgaagaac cttgatcgac ggatatttat tgttgtgttt tgcatgcgtc
      661 agcgtggact tgccggaacc tgacagacca tatacggatg caacaaattt agaaccgtct
      721 tcaagtgtat attccttctg gccgccgtgg caggaagcat aaccgtttct gtttgcaagt
      781 gcccagccca ttgtaagcgt acccttttta tgctcaccga aatatttcat tccgagaatt
      841 gcagcacagt tttggttggt atcaaaataa cataatgtca aaggatcatt caggcagctg
      901 taatcaacat ccggtgaagg ctgcggtacc cactgcggat ccgagaaaat ataaatatcc
      961 ggctcgttgc cgtctgcgat cggttttgat ttcttataaa gctttacata ctcatccgac
     1021 atatactgga agttgagcat ccagttataa agaagattct cttctccttc cggaataaga
     1081 agatgtgcct ttgccataaa ttcagcatcc agaccaataa aacattctgc atggtacatc
     1141 gtcttccagc gtgacttata tacggcatcc ataacaacct tgtcaagctt aacagtatca
     1201 acacccggct cgcctttaat gcggcgggca aatgcataac ggcctgttac ggctccgtcg
     1261 ttgaataaca gaactcttga atccttttca agaccaaatg tttcaccgtc cttgatcttc
     1321 atatcggtaa cgatcgttcc cggagaattt ttcgctaatt cataagcctc ttttaatgtg
     1381 tttactttta ctacgttgtt tccataaaaa gcagcctcaa taatggaacg ggttctggaa
     1441 aatccaacct tgccggcacc aatctctgct attggataat acgcttttgt tgacatagat
     1501 agtcctcctt tatatttaac ttattatcat aatccttgcc tattatctca aaatgaatat
     1561 taaaagtcaa gttgtaaaaa atgatttcac atcgatttta cgcttttttt agattttatg
     1621 cgtatgggct gcaagccacg ttttttcgct gtctgtgagg tgcggacaaa gcgtcctgaa
     1681 tacgtttgcg tggtagacat tcagtaaaat tttttctttt tctgacatta atttcggaag
     1741 caccgcatcc agatcaaacg gtaccagtgt cagtgtttca aatcctaaaa agcttcccca
     1801 ttccgtctct tctttcttga cacaaagcag ctcgttttcc agccggatcc catgacttcc
     1861 ttctatataa attccaggct cgtcactcgt gatcataccg gcctgcaggg gcggctgcaa
     1921 atgttctctg ttaattttaa agcgaaacgc ctgcggtcct tcatgaacat ttaaaaaact
     1981 tccgatccca tgtcccgttc catggcgata atcgatcccc agtctccaga tcggctctct
     2041 ggcaaggatg tccagattct cccctgtaca cccttccaaa aacctggcat ctgccaaatg
     2101 cagaagtcct ttcagaactg ccgtataatg ttccttttct tccatagtta actctccaag
     2161 cactatggta cgcgtaatgt ctgttgttgc accaagatac tgtccgcccg agtccacaag
     2221 cagcattccc ttcggatgca gcagtgcgct cccttccggt tttgcgctgt agtgtacaat
     2281 cgctgcattt tccttatacg cacagatcgt ttcaaatgac agaccaacaa acccctcgtt
     2341 cttttcccgc aaagcatcca aatattttgc agcttcgtat tcggtcacgt gtgccgtctt
     2401 cacctgttct ttaatatata taataaagcg aagaaccgaa actgcgtcga tcacatgata
     2461 ttttctggcg tggctgattt caatatcatt tttaacggct ttttctatca tctcagtatt
     2521 ctctccgaag agtaccctgt tcttttcttt catgcactga tacagccggc agtttaccat
     2581 ctgcggatca cacagaagcc tttccccgga caatttcaac aaaaatcctt cgatttcatc
     2641 gtatgacgca atccggactc caaatttcaa aagcacattt gcggtttctt tttcactccc
     2701 gtcttccggc agaaaaagga tcgcctgctc ctgcgaaata taagcataag aaaaagctac
     2761 cggattacag gcaatatcac tgccccgcaa attaaacagc cacataatac tgccaagctc
     2821 agtcagaaca taggcgtccg catccttctt tgcaagcagt cttcgaagct gtgcaagctt
     2881 tgttcccgta tctgaaccgg caatcgaaag atccagaaaa taaactccgt tttttgtcat
     2941 aggggggcgt tccggccaca ctctatctgc aaggtcaata tcagatcgta tcaatgcgcc
     3001 cgtttcttct gtaattttta aaagctccaa ataaaaagaa gtgctgattg tcctgccgtc
     3061 acagccgaca cacattccat acgcaagtac atccctaagg aactccctga tcgcaggcac
     3121 tcccttttcc tgcattctgt aaagttcgat ctttgtactg gcaagctctt tttctgcctg
     3181 cacataatat ctgccgtccg tccacaaaat cgccctgtca gccataacaa gcagcgtacc
     3241 tgccgatcct gtaaaccccg at
//
LOCUS       DAOSYE010000160         3246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21453_length_3246_cov_3.149483, whole genome shotgun sequence.
ACCESSION   DAOSYE010000160 DAOSYE010000000
VERSION     DAOSYE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21453_length_3246_cov_3.149483"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="PLU43_03765"
     CDS             complement(<1..1649)
                     /locus_tag="PLU43_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOO27557.1"
                     /translation="MDYMTIDSRPVPIEGEKNILSVIRKAGIDLPTFCYHSELSIYGA
                     CRMCVVEDDKGRIFASCSEQPRPGMVIQTNTKKIQQYRKLIIELLLSAHCRDCTTCTK
                     NGSCELQSLAYRVGVHAVRFLNNKKHQPLDMSSPSIVRDPNKCILCGDCVRTCSEIQH
                     VGALDFAYRGSKMQVMPAFNKTLAETDCVGCGQCRVVCPTAAISIRTNVTKVWDAITN
                     PKIRVVAQIAPAVRIAAGDRFGIPKGENSMGKMVSALRMMGFDSVYDTNFGADLTVVE
                     ESKELAERLEKEENLPLFTSCCPGWVKFCERKYPEFADHISTCRSPQGMFSAVVKEYY
                     KQKDAEENKTTYVVSIMPCTAKKAEILRPDNFTNGRQDTDSVLTTVEVIRMIRQVGIK
                     FEELDGEAPDMPFGMASGGASIFGVTGGVTEAVLRHFAKNKTHAALDEISFSGVRGTQ
                     GLKEATVKVGEREVKIAVVHGLGNAGELLDRIKAGEAQYDFVEVMACRGGCILGGGQP
                     LPMGPRTKKGRAEGIYRVDKESHIRFTSENPVLKQTYEEVI"
     gene            complement(1665..>3246)
                     /locus_tag="PLU43_03770"
     CDS             complement(1665..>3246)
                     /locus_tag="PLU43_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOO27558.1"
                     /translation="VKEEDCEEIYQTTILGGRPVERLLYEMDGVKYPAQEDIPFYAKQ
                     TRLVLKNCGHIDAEHIREAFAFGGYEALVKVLFEMKPQEVIDVVLSSNLRGRGGGGFQ
                     TGYKWKQVAKQEEKIRYVVCNGDEGDPGAFMDRSVMEGDPHKMIEGMIIAGYAVGAQE
                     GYIYVRAEYPLAISRLKIAIRQAEQCGLLGEDILGSGYSFKLHINRGAGAFVCGEGSA
                     LTASIEGERGMPRTKPPRTVEQGLFSKPTVLNNVETYANIPMIILNGAEWYHKIGTET
                     SPGTKAFAVTGSINNTGLIEVPMGTTLREIVYEIGGGIKNGKKFKAVQIGGPSGGCLI
                     DTDLDLPLDFDSLKKKGAMIGSGGLVIMDEDTCMVEVARFFMNFTQNESCGKCVPCRE
                     GTKRMLEMLEDIVGGRGTMEQLSMLEELGKAITDTALCGLGKSAALPVLSTLRVFRDE
                     YEEHVLDKKCRTGTCQALKRYVIDPALCKGCSKCARNCPVGAISGEIKSPFVIDANKC
                     IKCGACEAACPFHAITVE"
BASE COUNT          753 a          978 c          646 g          869 t
ORIGIN      
        1 ctgatcacct cttcataggt ctgtttcagc acaggattct cactggtaaa acggatatgg
       61 gattccttat ccacacggta gattccctcc gcgcgtcctt ttttcgtccg cggtcccatc
      121 ggaagcggct gacctccgcc gagaatacag ccgcctctgc acgccatgac ttctacaaaa
      181 tcatactgcg cttctccggc cttgattcgg tcaagcagct cacccgcatt gccaagccca
      241 tgtacaactg cgatcttcac ttcccgctcc ccgactttta cagttgcctc tttcagcccc
      301 tgtgtaccac gtacaccgct gaaactgatc tcatcgagtg ccgcatgtgt cttattcttt
      361 gcaaaatgcc gcaataccgc ttcagtcaca ccaccggtca caccaaaaat ggatgcaccg
      421 ccggacgcca tgccaaacgg catatccggt gcctctccgt ccagttcttc aaacttgatg
      481 ccgacctggc ggatcatacg gatgacttcc accgttgtca gtacactgtc cgtatcctgt
      541 ctgccgttcg taaaattatc cggccttaag atctcggctt tttttgccgt acacggcatg
      601 atcgatacga catacgtcgt cttattttct tcagcatctt tctgtttgta atattccttt
      661 acaactgcgg aaaacattcc ctgcggagaa cggcaggtag atatatgatc cgcaaattcc
      721 ggatactttc tttcacagaa tttgacccag cccggacagc aggatgtaaa gagcggcaga
      781 ttctcctctt tttcaagacg ctccgcaagt tctttcgatt cttccacaac agtcagatcc
      841 gcaccgaaat tcgtatcata aacagaatca aatcccatca ttcgcagcgc ggataccatt
      901 ttacccatgc tgttctctcc ttttggaata ccgaacctgt cgcctgccgc gatcctgacg
      961 gccggtgcga tctgtgccac aacgcggatc ttcggattcg tgatcgcatc ccacaccttt
     1021 gtaacattgg tcctgatgga gatcgctgcc gtaggacaaa caacacggca ctgtccgcag
     1081 ccgacgcagt ccgtttcagc aagcgtttta ttaaacgcag gcatgacctg catcttggat
     1141 cctctgtatg caaaatccag cgcaccaaca tgctgtattt cagaacatgt cctgacacag
     1201 tcaccgcaca ggatacactt gttggggtca cgcacgatac tcggcgaact catgtcgagc
     1261 ggctggtgct ttttattgtt taagaatcgg accgcatgca caccgacacg ataagccaga
     1321 ctctgcagct cgcacgaacc attcttcgta caggtcgtac agtctctgca atgtgcggaa
     1381 agcaaaagtt caatgatcag ctttctgtac tgctgtattt tttttgtatt ggtctgaatg
     1441 accatgcccg gccttggctg ctcggagcag gatgcaaaga tccttccctt atcatcctcg
     1501 accacacaca ttctgcatgc accataaatg gacagttccg aatgataaca gaacgtcggc
     1561 agatcgattc cggcctttct gattacagat aaaatattct tttctccctc gatcggaacc
     1621 ggacgggagt ctattgtcat ataatccatt gctttatcct cctatcattc caccgtaatc
     1681 gcatgaaatg ggcaagctgc ttcacaggcg ccgcatttta tacatttatt tgcatcaatt
     1741 acaaatggac tctttatttc tccgctgatc gcgcctacag gacaattcct cgcacacttt
     1801 gaacatccct tacagagcgc cgggtcgatc acatagcgtt tcaaagcctg gcaggtaccg
     1861 gtcctgcatt ttttatcaag cacatgttct tcatactcat cccgaaatac gcgcagtgta
     1921 cttaataccg gaagcgcagc actcttgccg agtccgcaca gtgccgtatc ggtgatcgcc
     1981 ttcccgagtt cttccagcat ggaaagctgc tccatcgtac ctctgccgcc tacaatatcc
     2041 tctaacatct ccagcatgcg ctttgtacct tcccggcacg gaacgcattt gccgcagctt
     2101 tcattctgtg tgaaattcat aaagaaacgg gccacttcca ccatacacgt atcctcgtcc
     2161 ataatgacca gaccgccgga accgatcatg gcgccctttt tctttaatga atcaaaatca
     2221 agaggcagat ccagatccgt atcgatcaga cagccgccgg aaggtccgcc gatctgcact
     2281 gccttaaact tctttccgtt cttgatcccg ccgccgatct cataaacgat ctcccgcagt
     2341 gttgttccca tcggcacttc gatcagaccc gtattattga tactgccggt cacggcaaac
     2401 gcttttgttc ccggactcgt ttcggtaccg atcttatgat accattctgc tccgttcaga
     2461 atgatcatcg gtatatttgc ataagtctca acattattga gtacggtcgg cttgctaaaa
     2521 agtccctgtt ctaccgtacg cggcggcttt gtacgcggca tgccgcgctc cccttcgatc
     2581 gaagcggtca gagcactgcc ttctccgcag acaaacgcac ctgcaccccg gttgatatgc
     2641 agcttaaaac tatatccgga gcctaaaata tcctcaccga gcagtccgca ttgttccgcc
     2701 tgtctgattg cgatcttcaa acgggaaatt gcaagcgggt attccgcacg tacataaata
     2761 taaccttcct gtgcgccgac cgcataacct gctatgatca tgccctctat catcttgtgc
     2821 ggatcacctt ccatgacgct tcggtccata aacgcacccg gatcaccttc atcaccgttg
     2881 cagaccacat aacggatctt ttcttcctgt tttgcgacct gtttccattt atatccggtc
     2941 tgaaagcctc cgcctccccg tccgcgcaga ttggacgaaa gaacaacatc aatgacctcc
     3001 tgcggcttca tctcaaacaa cacttttaca agagcctcat atccgccgaa cgcaaacgct
     3061 tccctgatat gctccgcatc aatatgtccg cagtttttca aaaccagacg tgtctgtttt
     3121 gcataaaacg gaatatcctc ctgcgccgga tatttgaccc cgtccatttc ataaagcagc
     3181 cgttccaccg gcctgccccc aagtattgtt gtttggtaaa tctcctcgca gtcctcttct
     3241 tttact
//
LOCUS       DAOSYE010000161         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21497_length_3242_cov_2.641983, whole genome shotgun sequence.
ACCESSION   DAOSYE010000161 DAOSYE010000000
VERSION     DAOSYE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21497_length_3242_cov_2.641983"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="pgmB"
                     /locus_tag="PLU43_03775"
     CDS             complement(<1..572)
                     /gene="pgmB"
                     /locus_tag="PLU43_03775"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOO27559.1"
                     /translation="MKYKAVIFDLDGVICHTDMYHYQAWKAVADKLGIYFDESINNRL
                     RGVSRMASFEIILERYKGTMSKEDKLRYTEEKNEIYKELLKNMGPQSLGTEVKDTLDR
                     LRAAGAKLAIGSSSKSAKFILGRLGLEGYFDAVSDGNNIINSKPDPEVFVKAARFIDV
                     PAAECLVVEDAKAGLEAAIKGGMDCAAIGEA"
     gene            complement(562..2862)
                     /locus_tag="PLU43_03780"
     CDS             complement(562..2862)
                     /locus_tag="PLU43_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOO27560.1"
                     /translation="MANNYRYELNDLSLENDALMLNETVFHNANGYIGVRSNFEEGYP
                     DGYDSIRSSYINGFYDFAEMKQAEKLYGFVEEKQTMLNVADTQGICLTIGDEVFSMFE
                     GTVLASRRYLDMERGYTVREVTWCSPKGHTIRITAKRMTSFDLLTLYTDEYTAESVDY
                     AGEVHLTSAHIGDVKNYSNLNDPRVAGESFEHLLPAEAKIEQGISYLTTDTSASNLRV
                     CTAVAHVISKDAEKEMWIEGHRVFARFDLTLKAGESLRLTKYTVFADSIRVKDTVEAA
                     KADMKQALSRPMSYWYEKQAEYLKRFWEQSSLEIDGDDDLSLAVRYNLYQLIQSVGKD
                     EHSNIAAKGLSGEGYEGHYFWDTEMYMQPFFTLTNPDVAKNLIAYRYSTMEAARDNAK
                     LLGHKKGVLYPWRTIMGKECSGYYPSGTAQYHINGDIAYSVIAYYLATKDLDFIAEKG
                     AEIVFETARLWIDVGNFYKGRFVINAVTGPDEYTCMINNNYFTNASAQYNLHWALRFA
                     ELLKKEGRFHALEEKLHLEREELKEFERAEKCMYLPYDEERGINPQDDSFLSKPVWDI
                     AATPKDRFPLLLHYHPLQLYRYQVCKQADTVLAHFIFEDIQSLETIRRSFDYYEKVTT
                     HDSSLSTCIYSIVASKLGLKEKAYDYFGDSAKLDLFNTHNNTKDGIHTANMGGNYMAI
                     VYGFAGLRLKEDGIHFAPSLPAQWSGCRFKINYQGSQIYVDIKKEEVTFELMHGEKQQ
                     IHVFDKTYELTDKIVIREGRGGRDEV"
     gene            complement(2873..>3242)
                     /locus_tag="PLU43_03785"
     CDS             complement(2873..>3242)
                     /locus_tag="PLU43_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27561.1"
                     /translation="VFLYHGFIKSIPVSLEEAATIDGCTKLGVFWKIVFPMLKPTTMT
                     VIILDVIWIWNDYLLPSLVLSSKANRTIPLSTFSFFGQFTIQWNLAMAGLMLTIIPVI
                     IFYLCAQKYIIKGVAAGAVK"
BASE COUNT          853 a          846 c          607 g          936 t
ORIGIN      
        1 gcctctccaa tggctgcaca atccataccg cctttaatgg ccgcctctaa tccggcttta
       61 gcatcctcta caacaaggca ttcggccgca ggaacatcta tgaaccgggc cgcttttacg
      121 aacacctcag ggtctggttt ggaatttata atattgtttc catctgaaac cgcatcgaaa
      181 tatccttcca gtcccagacg gcctaaaata aattttgcac ttttgctgga agaaccaatt
      241 gcaagcttag cacctgctgc ccgcagtctg tccagtgtgt cctttacctc tgtgccaaga
      301 ctctgaggtc ccatattttt taataattct ttatagatct cattcttttc ttccgtatag
      361 cggagcttgt cttcttttga catggtccct ttatagcgct ctaaaataat ttcaaagctg
      421 gccatacggc ttactccgcg aaggcggtta ttaatactct cgtcaaagta aatgccaagc
      481 ttatccgcaa ctgccttcca cgcctgataa tgatacatat cggtatggca gatcacacca
      541 tccagatcaa agatcactgc cttatacttc atcccggccg cctctccctt ctcttatcac
      601 aattttgtcc gtcaattcat atgttttatc aaatacatgg atttgctgtt tttccccatg
      661 catcaattca aaagtcactt cctctttttt aatatctaca tagatctgac ttccctgata
      721 attgatctta aaacggcagc cgctccactg tgccggaagt gacggcgcga aatgaatgcc
      781 gtcttctttc aggcgaagtc ctgcaaatcc gtacacgatc gccatatagt tcccgcccat
      841 attagcagta tgaataccat ccttcgtatt gttatgtgta ttgaacagat ccagctttgc
      901 cgaatctcca aaataatcgt atgctttttc tttcagtcca agcttgctgg ctacaatact
      961 atagatgcat gtggaaagag aagaatcgtg cgttgtcact ttctcataat aatcaaaaga
     1021 cctgcgaatc gtttcaagcg actggatatc ctcaaatata aaatgggcca gtacggtatc
     1081 cgcctgttta cacacctgat aacgatataa ctgcaatgga tgataatgca gtaacaatgg
     1141 gaacctgtct tttggtgtcg ctgcaatatc ccatacgggc ttgctcagaa aagaatcatc
     1201 ctgtggattg ataccgcgtt cttcatcata tggaagatac atgcattttt ctgccctttc
     1261 aaactcctta agctcttccc gttccaaatg gagcttttct tccagcgcat ggaatctgcc
     1321 ctcttttttt aacaattccg caaaacggag cgcccagtgc agattgtact gtgcagatgc
     1381 attcgtaaag taattattat tgatcatgca agtatattca tctggtccgg tcactgcatt
     1441 gatcacaaag cgtcccttat aaaaattacc gacatctatc cagagcctcg ctgtctcaaa
     1501 cacgatctcc gcaccttttt cagcaataaa atccagatct ttagttgcaa gataataagc
     1561 aattacagaa tacgcaatgt ctccgttgat atggtactgt gccgtacctg acggataata
     1621 gccggagcat tcttttccca taatcgtccg ccatgggtat aacacccctt tcttatgtcc
     1681 caggagcttc gcattatctc tggctgcttc catcgtacta taacgatagg cgatcagatt
     1741 cttagcgaca tccggatttg tcagggtaaa aaacggctgc atgtacattt cggtatccca
     1801 gaaataatgt ccctcatagc cctcaccgct cagtcctttt gccgcaatat tgctgtgctc
     1861 atctttgcca accgactgga tcagctggta cagattatag cggactgcaa ggcttaagtc
     1921 atcgtccccg tcgatctcca gagaggattg ttcccaaaac cgtttcagat actccgcctg
     1981 tttttcatac cagtaagaca tcggtcgcga aagtgcctgc ttcatatccg ccttagccgc
     2041 ttctacggta tctttcacgc gaatgctgtc tgcaaagacc gtgtattttg tcagccgcag
     2101 gctttcgccc gctttcagtg tcaaatcaaa ccgggcaaat acacgatgtc cctcgatcca
     2161 catttccttt tctgcatcct tggagatcac atgagctacc gctgtgcata cacgcagatt
     2221 tgaagccgat gtatctgttg tcagataaga aattccctgc tctattttcg cctccgccgg
     2281 cagcagatgt tcaaagctct cacctgctac ccgcggatca tttaaattgg aataattttt
     2341 cacatcgccg atatgggcag atgtcagatg tacttcacct gcgtagtcta cagactctgc
     2401 ggtatactca tccgtgtaca gggtcagaag atcaaatgaa gtcatacgct ttgccgtgat
     2461 cctgatcgta tggcccttcg gagaacacca ggtcacctca cggaccgtat agcctctctc
     2521 catatcaagg tatcttctgc ttgccaatac cgttccttca aacatggaaa atacttcatc
     2581 cccgattgtc aggcagattc cctgcgtatc cgcaacattc agcatcgtct gcttttcttc
     2641 tacaaatccg tacagttttt ctgcctgctt catttcagca aagtcataaa acccattgat
     2701 ataggaactg cggatagagt catatccatc cggatatcct tcctcaaaat tggaccggac
     2761 cccgatatat ccgtttgcat tatggaacac agtctcattc agcatcagag catcattttc
     2821 caagctcaga tcgttcagct catatctata attattagcc atcgtccgct ccttacttca
     2881 cagcacctgc tgcaacacct ttgatgatat atttctgggc acacagatag aaaatgatga
     2941 ccgggataat tgtcagcatc aggcctgcca ttgcaagatt ccactgaatc gtaaactgtc
     3001 cgaaaaagga aaaggtagac agcggaatcg ttctgttcgc tttgctcgaa agaaccagag
     3061 agggaagcag gtaatcgttc cagatccaga tcacatccaa aataatgact gtcatcgtag
     3121 taggtttcag cattggaaac acgattttcc aaaacacacc caatttagtg catccgtcga
     3181 tcgtagcagc ttcctcaaga gagaccggaa tggacttgat aaatccatgg tacaaaaaga
     3241 ca
//
LOCUS       DAOSYE010000162         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21619_length_3230_cov_2.974173, whole genome shotgun sequence.
ACCESSION   DAOSYE010000162 DAOSYE010000000
VERSION     DAOSYE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21619_length_3230_cov_2.974173"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLU43_03790"
     CDS             complement(<1..420)
                     /locus_tag="PLU43_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO27562.1"
                     /translation="MKKLDTNDWLVLNSIIYKIYTTEDERKMRASFLEHLKMIIDFDA
                     ADFYLASEISETGLVDPIAYNCDCVSGEYDELDYSRGILYSGKSMVYRETDIIDDEKR
                     KISEYYKKVYKPNNWHYSMQIILARNKQLLGVATLYRT"
     gene            complement(398..1465)
                     /gene="aguA"
                     /locus_tag="PLU43_03795"
     CDS             complement(398..1465)
                     /gene="aguA"
                     /locus_tag="PLU43_03795"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HOO27563.1"
                     /translation="MAERITGTTPKKDGFRMPGEFEPQEKVWMIWPERPDNWRNGGKN
                     AQKAFGDVACAVSRFAKVTMLVSQAQYDNCRNRLPKHIAVVEMSSNDAWARDVGPTFL
                     ISETGELRACDWKFNAWGGFVDGLYAPWNLDDQVARKICELEQADSYRTDDFVLEGGS
                     IHVDGEGTVLTTEMCLLSAGRNPQMSKQEIETVLCEYLNCSKVLWIKDGIDPQETNGH
                     IDDVACFVRPGEVACIDTKDETSPFYHAAAAACEQLGSMTDARGRQLKVHRICCPKKE
                     VRFGEDFLVDRVTGSVLRQAGDICIASYLNFLIVNEGVIVPQYGDENDRLALEQIQRI
                     FPEKKVVGVFTREIYEKTGYQ"
     gene            complement(1511..2371)
                     /gene="aguB"
                     /locus_tag="PLU43_03800"
     CDS             complement(1511..2371)
                     /gene="aguB"
                     /locus_tag="PLU43_03800"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HOO27564.1"
                     /translation="MKVVKAAATQMSCCTDRKQNLQTAEDMVRKAAALGAQIILLQEL
                     FETPYFCQKQDYQYFNLAAAPEENPAVRRFREVAKELNVVIPVSFYEKAGNTAFNTVA
                     VIDADGTVLGYYRKTHIPDGLPYAEKFYFTPGDTGFQVWDTRYAKIGIGICWDQWFPE
                     AARSMALLGAELLFYPTAIGSEPDLKLDSKDHWQRCMQGHSAANIMPVIASNRIGRET
                     EPDSEMTFYGSSFITDESGKIVQEAGREEQTVLVQEFDLDQIARRRREWGVFRDRRPE
                     MYGILSTHGN"
     gene            complement(2525..2848)
                     /locus_tag="PLU43_03805"
     CDS             complement(2525..2848)
                     /locus_tag="PLU43_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27565.1"
                     /translation="MFRKNLVSLQLRKKIALALMAISLSSVLLYGCGSSSADDSTSEE
                     TTVETTDETTDETEAADETEATDGEAPDGEAPEGEAPEGGEQGEKPADAPEKPADDAS
                     TETAE"
BASE COUNT          791 a          867 c          571 g         1001 t
ORIGIN      
        1 cgtccggtaa agagtcgcta ctccaagcag ctgtttattt cttgccagaa tgatctgcat
       61 ggaataatgc cagttgttcg gtttgtatac ctttttataa tattcactga tcttacgctt
      121 ttcgtcgtca ataatatctg tttcacgata gaccatactt ttcccactgt aaagaattcc
      181 cctgctgtaa tccagttcgt catattctcc ggacacgcag tcacaattat atgcgatcgg
      241 atccacaagt cctgtttcgg aaatttccga agcaagataa aaatctgccg catcaaaatc
      301 aatgatcatt ttcaaatgtt ccaaaaatga agcacgcatt ttccgctcgt cttccgttgt
      361 gtatatttta tagataatgc tgtttaacac aagccagtca ttggtatcca gttttttcat
      421 atatttctct tgtaaagacc ccgaccactt ttttttccgg aaagatcctc tggatctgtt
      481 ccaatgccag ccggtcgttt tcatccccgt actgcggaac gattacgccc tcattcacga
      541 tcaaaaaatt cagataagac gcaatacaaa tatcgcctgc ctgtcttaaa acggagccgg
      601 tcacacgatc caccaaaaaa tcttctccaa atctcacttc ttttttagga cagcagatcc
      661 tgtgtacctt taactgtctt cccctggcat ccgtcatgct gccaagctgt tcacatgccg
      721 ctgctgccgc atgataaaat ggagacgtct cgtccttagt atcgatacag gccacttcgc
      781 cgggtcttac aaaacatgca acatcatcaa tatgtccgtt cgtttcctgc ggatcgattc
      841 catccttaat ccaaaggact ttgctgcagt tcaaatactc acaaaggacc gtttcgattt
      901 cctgctttga catctgcgga ttgcgtcctg cactcagaag acacatttcc gtcgtcagca
      961 ccgttccttc tccgtccaca tgaatagaac ctccctccaa tacaaaatca tccgtacggt
     1021 aggagtctgc ctgttccagt tcacagatct ttcgcgcaac ctgatcatcc agattccatg
     1081 gcgcatacag tccgtcaaca aagccgcccc atgcattaaa tttccagtca caggccctaa
     1141 gttcccctgt ttcgctgatt aaaaaagtag gaccgacatc tcttgcccat gcatcgttac
     1201 tgctcatctc aacaaccgca atatgctttg gaagccgatt ccggcaattg tcatactgtg
     1261 cctgggatac gagcatggtt actttggcaa accggctgac cgcacatgcc acatcgccaa
     1321 atgctttctg tgcatttttc ccgccgttcc tccaattgtc cggccgctcc ggccagatca
     1381 tccaaacttt ttcctgtggt tcaaattccc ccggcatccg gaaaccgtct ttctttggtg
     1441 tcgtacccgt tatcctttcc gccatgactt cattcctttc ttcctaaaat aatacctgtt
     1501 tttcatattt ctaattcccg tgagtcgata gtatcccata catttccggt cttctgtcac
     1561 ggaatacacc ccactccctt cttcttcgtg cgatctgatc cagatcaaac tcctgtacaa
     1621 gaaccgtctg ctcctctctt cctgcctcct gcacgatctt tccgctttcg tccgtaataa
     1681 aggaagaacc gtaaaatgtc atctcagaat ccggttctgt ttcccttccg atccggttcg
     1741 atgcaatgac cggcataata ttggcagcgc tgtgcccctg catacacctt tgccaatgat
     1801 cctttgaatc cagcttcaga tccggttcac tgccgattgc cgtaggataa aagagcagtt
     1861 ccgctccaag aagtgccatg gatcttgccg cttccgggaa ccactgatcc cagcaaattc
     1921 caataccaat tttggcatat cgggtatccc atacctgaaa gcccgtatct cccggcgtaa
     1981 aataaaattt ttccgcatac ggcagtccgt ccggtatgtg cgttttcctg taatacccca
     2041 gtaccgttcc gtcggcatcg atcacagcaa ccgtattgaa tgcggtattt cccgcttttt
     2101 cataaaaact caccggaatt accacgttca gttcctttgc cacttcccga aaacgcctga
     2161 cggccggatt ctcttccggc gccgcagcca gattaaaata ttgataatcc tgcttttggc
     2221 aaaaatacgg tgtttcaaat aactcctgca gcaaaatgat ctgcgcaccc aacgccgccg
     2281 ctttcctgac catatcctcg gcagtctgca gattctgctt tctgtctgta cagcaggaca
     2341 tttgtgttgc cgccgccttt accactttca tatgaatacc tcctgacaat ctttccatga
     2401 tagaaaaaaa gaagcagaac tataaaagtt tctgcttctt tgcaactttg atataaccac
     2461 attatacatc aaattgctta acaggcaata ccctgtttga tttttctgtt tttctttttt
     2521 ctttttattc tgctgtttcc gtactggcat catcagccgg cttttccggt gcatctgcag
     2581 gcttctctcc ctgctctcct ccttctggtg cctctccttc cggcgcttct ccgtcagggg
     2641 cttctccgtc ggttgcttct gtttcatcgg ctgcttccgt ctcatcagtc gtttcatcag
     2701 ttgtttccac cgttgtttct tcagaagtgc tgtcgtccgc agacgaactg ccgcatccat
     2761 aaagcaatac ggatgagagt gatattgcca ttaaggcaag tgcaattttt tttcgtaatt
     2821 gtaacgatac taaatttttt ctgaacatat tttcctccat aataatcctg atttgaagag
     2881 aactgccaag ctgttggttc tcttcctgat tacgttcaca tattgccata taattgtgaa
     2941 aaaattatgt tataattatg aactattgac gttcataacc tgaaaaacag cctttttaga
     3001 tactgtgtcc gatacatttt ttagtatctt tttcgatatt aaaaaacgcg ctgaaatttg
     3061 acagcgcctg cggttttttg ttattatgtt aattgaaaag attaccatga ataaactgat
     3121 tgtttacaaa aagaaaggag caattacaaa tgaaagaaat tcatatggga attggttttg
     3181 caaccgggcg ccgaaatttc cggaaagttt tgtctgctta tatcaattcc
//
LOCUS       DAOSYE010000163         3229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_21632_length_3229_cov_2.892880, whole genome shotgun sequence.
ACCESSION   DAOSYE010000163 DAOSYE010000000
VERSION     DAOSYE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21632_length_3229_cov_2.892880"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLU43_03810"
     CDS             <1..321
                     /locus_tag="PLU43_03810"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO27566.1"
                     /translation="PTQLLSSGGDFLLAIGLMIYARRAKYRGNVSAFYLLFYSVGRFM
                     VEIFRGDDRGTVGFLSTSQFIAVLMFLFALAFLFYNKKRGFEAEKYTPGKVQPKDEDP
                     KKEA"
     gene            325..1680
                     /locus_tag="PLU43_03815"
     CDS             325..1680
                     /locus_tag="PLU43_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27567.1"
                     /translation="MQYQNDYEKMTKMPVEKLIIRLGLPTTISMLVTNIYNIADTYFV
                     SQLGTSASGAVGIIFALMAVIQAFGFMLGHGAGSNISRRLGARKVEEAKIFASTSFFL
                     AVGCGLLLLLFGIIFLTPLARLLGSTETILPYAKVYALFILLSAPAMTTSCVMNNILR
                     YEGKAFYSMIGLVSGGVLNIFGDYLLIMKCHFGIAGAGISTAVSQYISACILLCPFLI
                     RKTQSCFSLKYFTKKAAVIKSILFIGFPSMLRQGLASVSVMLLNVQAAVYGDAAIAAM
                     SIASRVINLLFCVGIGIGQGFQPVSAFNYGAGLYSRVKKGAIFAFLFGTLLMGIFAVF
                     GFAEAEPVVTIFRDDPAVVEIGTVALRAQCIALFFIPLTACASMLFQSVGEGKKASLM
                     ASLRSGVCFVPAILILPAFIGIRGIQYAQLFADVLSFMITLPLVIRFFKTLPQDKAEK
                     C"
     gene            1698..2111
                     /locus_tag="PLU43_03820"
     CDS             1698..2111
                     /locus_tag="PLU43_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27568.1"
                     /translation="MAGRAFTCLAKTMDLIVFLAAAVTVGILSYEGLTGMEYGKVLSA
                     AVTVMDNAFLISVIMHFIKREGSMVLVCFNLINTGLLVLAYLLLFIKIEYAPWMLLFW
                     DMYLLIYYGYKTTLIIQEKRGEKENGSSDQKVEKE"
     gene            2077..2601
                     /locus_tag="PLU43_03825"
     CDS             2077..2601
                     /locus_tag="PLU43_03825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO27569.1"
                     /translation="MEAVIRKWKKSDAKSVAKYANNENIAKNLRNVFPYPYTLADAEG
                     YVYDCVERGDDRRLVRAIEVNKEAVGSIGIFLGCDVAEKSAELGYWLAEKYWKQGIMS
                     SAVKQLCAEAFLTFDLVRIYAEPFSYNEGSKAVLKKAGFTYEGTMRSSVFKNGRMFDQ
                     CLYSLLKNEIGSIH"
     gene            2733..2867
                     /locus_tag="PLU43_03830"
     CDS             2733..2867
                     /locus_tag="PLU43_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOO27570.1"
                     /translation="MKKYKFFAILNAFAVALAVQNLEWACFWFVYQPRVPEELAKFKK
                     "
BASE COUNT          903 a          546 c          841 g          939 t
ORIGIN      
        1 ccgacgcagc tgctttcttc gggcggtgat ttcctgctgg cgatcggttt gatgatctat
       61 gcaagacgtg cgaaatatcg tggaaatgtt tcggcttttt atctgctctt ttatagtgtc
      121 ggcagattta tggtcgaaat attcagaggg gatgaccgcg gaacagtcgg gttcctgtcc
      181 acttctcagt ttattgctgt tctgatgttc ctttttgcgc tggcgtttct gttttataat
      241 aaaaaacggg gatttgaggc tgaaaagtat acgccgggaa aagtacagcc aaaagatgag
      301 gatccgaaaa aagaggcata atagatgcag tatcagaatg attatgaaaa aatgacaaag
      361 atgccggtag aaaagctgat tatccggctc ggactgccga ctacgatcag tatgctcgta
      421 acgaatattt ataatattgc agatacgtat tttgtcagtc agctcggaac gagtgccagc
      481 ggtgccgttg gaattatttt tgcactgatg gcggtcatac aggcctttgg gttcatgctg
      541 gggcatgggg ccggcagcaa tatcagtagg cgtctggggg caaggaaggt ggaagaagca
      601 aagatttttg cttcgaccag cttcttttta gctgtcggat gcggcctgtt attactgctg
      661 tttggaatca tatttctgac tccgctcgca aggctgcttg gaagtacgga aacgatactg
      721 ccttatgcaa aagtgtatgc gctgtttatt ctgctttcag ccccggcaat gacgacgagc
      781 tgcgtaatga ataatattct gcgttatgag gggaaggcat tttattcgat gatcggactt
      841 gtatcgggag gagttctgaa tattttcggt gattacctgc tgatcatgaa gtgccacttt
      901 ggtattgccg gagccggtat ttcgactgcg gtctcacagt atatcagtgc atgtatcctg
      961 ctttgtccgt ttcttatcag gaaaacacag agctgttttt ccctaaaata ttttacaaaa
     1021 aaagcagccg ttataaaaag tattttattt atcggatttc cgagcatgct ccgccagggt
     1081 ctggcgagtg tttccgtcat gcttttgaat gtgcaggcag cggtttacgg ggacgcggcg
     1141 atcgcggcga tgagtattgc gtcaagagtg atcaacctgc tgttctgtgt tggtatcggt
     1201 atcgggcagg gatttcagcc ggtgtcggcg tttaattatg gtgcgggtct ttattcgcgt
     1261 gtaaaaaagg gagcaatatt cgcttttttg ttcggaactt tgctgatggg catttttgcg
     1321 gttttcggat ttgcggaagc agaacctgtt gtaacgatat tcagggatga tccggcagta
     1381 gtcgaaatcg gaacagttgc gctgcgcgct caatgcatcg ctttattttt tattccgctt
     1441 acggcctgtg caagcatgct gtttcagagt gtcggagagg ggaagaaggc atcgcttatg
     1501 gcaagccttc gcagcggggt ctgttttgta ccggcgatct tgattttacc ggctttcatc
     1561 gggatacgcg ggatccagta tgcacagctt tttgcggatg tactttcttt tatgatcacg
     1621 cttccgcttg tgatacggtt ttttaaaaca ctgccgcagg ataaggcaga aaaatgctga
     1681 caaaggagga ataaacaatg gcagggagag cttttacatg tttggcaaag accatggatc
     1741 tgatcgtatt tttagcggca gccgtgacgg tcggtatttt atcgtatgaa gggctgacag
     1801 gaatggaata tggaaaagtt ttgtctgctg cggtcacggt gatggacaac gcgtttttaa
     1861 tatctgttat tatgcatttt ataaagcggg aaggctccat ggtattggtc tgctttaatc
     1921 ttatcaatac cggcttgctc gtactggcat atctgctgct gtttattaaa atagaatatg
     1981 ctccatggat gctgcttttt tgggatatgt acctgctgat ctattatgga tataaaacga
     2041 cgctgataat acaggaaaag agaggggaaa aagaaaatgg aagcagtgat cagaaagtgg
     2101 aaaaagagtg atgcaaaaag tgttgcaaaa tatgcgaata atgagaacat agcaaaaaat
     2161 ttaagaaatg tgtttccata tccgtatacg ctggcggatg cggaaggcta tgtatatgac
     2221 tgtgtggaga gaggagatga ccgccggctt gtacgggcaa ttgaagtgaa caaagaggcg
     2281 gtgggcagca tcggcatttt tttaggctgt gatgttgcgg aaaaaagtgc ggagcttggt
     2341 tactggcttg cggaaaaata ttggaagcag ggaatcatgt caagtgcggt aaaacagttg
     2401 tgtgcagaag cgtttttgac atttgatctt gttcggatct atgcggaacc gttttcttat
     2461 aatgagggat ctaaagcggt gctgaaaaaa gcaggtttta cttatgaagg tacgatgagg
     2521 agcagcgttt tcaaaaatgg ccggatgttt gatcagtgtt tgtattcgct gctaaaaaat
     2581 gagatcggca gcatccatta aattacaaat atatactaaa acagtaattt tcatgtaaaa
     2641 ttggcttcaa aacgtgtaaa aaacgctgcg cccattgaat taccagctga atttatataa
     2701 agtaaaagaa agctgggaag gagggatcgc ttatgaagaa atataagttt tttgccatat
     2761 tgaatgcttt tgctgtggca ctggcagttc agaacctgga atgggcatgt ttctggttcg
     2821 tatatcagcc aagagtaccg gaagaacttg ccaaatttaa aaagtaacaa aaatgagttc
     2881 ggatttagtt acaatagaag cctgtacggg attatctgta aaaacagtat ggttatgaac
     2941 cggaaagttc cataaatgtt tttaggagtc agagtatcct gtacaggttt tttatctgca
     3001 taaaagtaaa ataaacaaag aaaaaaagga aaaaagtttg attaatattg tgaaaagcac
     3061 aaaaaactta ataaaattta attattttaa aaaaaacgat tgacaaatcg gaaaaaaatg
     3121 ataaaattac aacttgtgaa agcaaaggcg cttggacaat tccaggcgcc tttctgcagt
     3181 atgacgggca tgtcccgaac cttgggtgaa agtcccaaag ggcgtagtt
//
LOCUS       DAOSYE010000164         3195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22027_length_3195_cov_2.634076, whole genome shotgun sequence.
ACCESSION   DAOSYE010000164 DAOSYE010000000
VERSION     DAOSYE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22027_length_3195_cov_2.634076"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /gene="rpsK"
                     /locus_tag="PLU43_03835"
     CDS             <1..303
                     /gene="rpsK"
                     /locus_tag="PLU43_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO27571.1"
                     /translation="TIVTITDSEGNALSWASAGGLGFRGSRKSTPYAAQMASETATKA
                     ALIHGLKSVDVMVKGPGSGREAAIRALQACGLEVTSIRDVTPVPHNGCRPPKRRRV"
     gene            374..967
                     /gene="rpsD"
                     /locus_tag="PLU43_03840"
     CDS             374..967
                     /gene="rpsD"
                     /locus_tag="PLU43_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO27572.1"
                     /translation="MAVNRVPVLKRCRSLGLEPMYLGIDKKSKRTLTRGSRKISEYGL
                     QLREKQKAKFIYGVLEKPFRNYYEKADRMKGMTGENLMMLLECRLDSVIFRMAFARTR
                     REARQIVDHKQVSVNGKTVNIPSYLVKAGDVIEIREKCKASQRYKDIVEVTKGRLVPE
                     WLDVDQENLKGTVKELPTRAEIDVPVDEMLIVELYSK"
     gene            998..1957
                     /locus_tag="PLU43_03845"
     CDS             998..1957
                     /locus_tag="PLU43_03845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOO27573.1"
                     /translation="MFDFEKPKIEIVEISEDKKYGKFVVEPLERGYGTTLGNSLRRIM
                     LSSLPGAAVSQIKIDGVLHEFSSISGVKEDVAEIIMNIKSLAIKNSSTTNEPKTAFIE
                     FEGEGVVTAADIQVDQDIEILNPELPIATLNGGSDCKLYMELTITKGRGYVSADKNKT
                     EDLPIGVIAVDSIYTPVERVNMTVENTRVGQITDFDKLSLDVFTNGTLAPDEAISLAA
                     KVLSEHLSLFIDLSENAKTAEVMIEKEDNEKEKVLEMNIDELELSVRSYNCLKRAGIN
                     TVEELTNRTSEDMMKVRNLGRKSLEEVLAKLKELGLQLNVGDE"
     gene            2140..2676
                     /locus_tag="PLU43_03850"
     CDS             2140..2676
                     /locus_tag="PLU43_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOO27574.1"
                     /translation="MAKYRKLGRTSSQRKALLRNQVTNLIYHGKIVTTEAKAKEIRKI
                     AEGLIALAVKEKDNFETVKIQAKVAVKDKDGKRVKEVIDGKKVTKFDVVEKEIKKDLP
                     SRLHARRQILRVLYPIVEVPSAPAGRKRNTKEVDLVAKLFDEIAPKYVNRKGGYTRIV
                     KIGQRKGDAAMEVLIELV"
     gene            complement(2741..>3195)
                     /locus_tag="PLU43_03855"
     CDS             complement(2741..>3195)
                     /locus_tag="PLU43_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO27575.1"
                     /translation="NGNKCTGELICNKEIAPDLVEIFYELYKADYPIEKIKLIDDYGG
                     DDEASMADNNSSCFNYRVIAGTDRLSLHALGMAVDINPLYNPYITYKNGSENIAPANA
                     AAYADRNADFAGKITHDDLAYKLFTAHGFTWGGDWKTTKDYQHFQKSE"
BASE COUNT         1032 a          547 c          816 g          800 t
ORIGIN      
        1 acgatcgtaa cgattacgga tagtgaagga aatgcccttt catgggcaag tgccggtggt
       61 ctgggtttta gaggttcaag gaaatctact ccatatgcag cacagatggc gtcggaaacg
      121 gctaccaagg ctgctttaat acatggctta aaaagtgtcg atgttatggt gaagggtcct
      181 ggatcaggaa gagaagctgc aatccgtgca ttgcaggctt gtggtttgga agtaacaagt
      241 atcagagacg taacgccggt tccgcataat ggatgccgtc cgccgaaacg cagaagagtt
      301 taatggtaac taaaataatg aaagttggaa gaaggtccgt tcaggattgt aaacaattaa
      361 tggaggaaaa gaaatggcag taaacagagt tccggtattg aaaagatgca gatcacttgg
      421 tttggaaccg atgtacttgg gaattgacaa aaaatcaaag agaacattga ccagaggaag
      481 cagaaaaatc agtgaatatg gtcttcagct gcgagaaaag cagaaggcaa agtttattta
      541 tggagtgctt gaaaagccct tcagaaatta ttacgaaaaa gccgacagaa tgaaaggcat
      601 gacaggtgaa aatctgatga tgcttcttga gtgcagactt gacagcgtta ttttcagaat
      661 ggcgtttgcg agaacgagaa gagaagcaag acagatcgtt gaccataagc aggtttccgt
      721 aaatggaaaa actgtaaata ttccgtctta tcttgtaaaa gcgggtgatg taattgaaat
      781 cagagaaaaa tgcaaagctt cacagagata caaagatatc gttgaagtta cgaaaggaag
      841 acttgttccg gaatggcttg atgtggatca ggaaaatttg aaaggaactg taaaggaact
      901 tcctactcgt gcggagatcg atgtaccggt tgatgagatg cttatcgtcg agttatattc
      961 taaataatca gtatccgaaa aaaggaggta ctttgcagtg tttgattttg aaaagccgaa
     1021 aattgaaatt gttgagattt cagaagataa aaagtacgga aaatttgttg ttgaaccgct
     1081 tgaaagaggc tatggcacga cactaggcaa ttcgctcaga aggattatgc tttcttccct
     1141 gccgggtgcg gcagtcagcc agatcaaaat agacggtgtt ctgcatgagt tcagttccat
     1201 ttccggtgta aaggaagatg tagcggaaat catcatgaat attaagagtc ttgcaataaa
     1261 gaacagcagt acgacaaatg agccgaagac ggcctttatc gaatttgaag gcgaaggtgt
     1321 tgtgactgct gccgatattc aggtggacca ggatattgaa atcttaaatc cggagcttcc
     1381 gatcgctaca ttgaacggcg gttccgactg taagctgtat atggagctta cgatcacaaa
     1441 aggaagaggt tatgtaagcg ccgacaaaaa taaaacggaa gatctgccga tcggagtgat
     1501 tgcggtggat tccatttata cacctgttga gcgtgtcaat atgacagttg agaatacccg
     1561 tgtcggtcag atcacggatt ttgacaaact gtcgttagat gtgtttacaa atggtacatt
     1621 ggcaccagat gaggcaatca gtctggcagc aaaggtgctg agtgaacatc tttctctttt
     1681 tatcgattta tcagaaaatg caaaaacagc ggaagttatg atcgaaaaag aagataatga
     1741 gaaagaaaaa gtactcgaga tgaacattga cgagcttgaa ctttccgttc gttcctataa
     1801 ctgtttaaag agagcaggta tcaatacggt tgaggagctg acgaacagaa cgtcagagga
     1861 tatgatgaag gttcgtaatc ttggacgcaa gtctttggaa gaggttcttg caaaattaaa
     1921 agagctcggt ctgcagttaa acgtagggga cgaataactg ctgatgcaga cggaggaagc
     1981 caactcagta aagccaaatg gtatgaggag gtatgcgctt tttgtaaagg cgctgtaact
     2041 attaatgatg ccagacaggc agtattcggt aagtaagtcc gatgtgcata gccggatatc
     2101 gatcagtgtg cacgtatgtg cacgattttg gaggaaacca tggcaaaata cagaaaactt
     2161 ggcagaacat ccagccagag aaaagcatta cttagaaatc aggtaacaaa tctgatttat
     2221 cacggaaaga tcgttacgac agaagcaaag gcaaaggaga tcagaaagat cgccgaaggt
     2281 ctgatcgcac ttgccgtaaa ggaaaaagat aattttgaga ccgtcaagat ccaggcaaag
     2341 gttgctgtta aggataaaga cggcaaaaga gtgaaagaag tgatcgacgg caagaaggta
     2401 acaaaattcg atgtcgttga aaaagagatc aaaaaagatc tgccgtccag actgcatgca
     2461 agaaggcaga tactcagagt tctttatccg atcgtggaag taccgtcagc gccggccgga
     2521 agaaaaagaa atacaaagga ggtcgatctg gtggcaaagc tttttgacga gatcgcaccg
     2581 aagtatgtaa atcgtaaggg cggttatacg agaatcgtta agatcggaca gcgtaaaggt
     2641 gatgcagcaa tggaagtact gatcgagctc gtataggttt tatttagcaa tagtggatcc
     2701 tgccggatat catccggcag gattttatat tatatatcgt tcattcggat ttttgaaaat
     2761 gctgatagtc ctttgtagtt ttccaatcac cgccccaggt aaagccatgc gcggtaaaaa
     2821 gtttgtaagc cagatcatca tgtgtgatct ttccggcaaa atcagcattt ctgtccgcat
     2881 atgcggcagc attggcaggt gcgatatttt cggaaccatt tttataggtg atgtaaggat
     2941 tataaagcgg attgatatcc acagccatgc cgagcgcatg gagagaaagc ctgtctgtac
     3001 cggcaatgac acgataatta aaacaggagg aattgttatc tgccatggac gcttcgtcgt
     3061 ctccgccgta gtcgtcgatc agttttattt tttctatcgg atagtctgcc ttatataact
     3121 cataaaaaat ttcaacaaga tccggtgcta tttccttgtt gcagatgagt tctcccgtac
     3181 atttatttcc gttga
//
LOCUS       DAOSYE010000165         3189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22092_length_3189_cov_2.594448, whole genome shotgun sequence.
ACCESSION   DAOSYE010000165 DAOSYE010000000
VERSION     DAOSYE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22092_length_3189_cov_2.594448"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="hisD"
                     /locus_tag="PLU43_03860"
     CDS             <1..875
                     /gene="hisD"
                     /locus_tag="PLU43_03860"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOO27576.1"
                     /translation="STLMCVLPAKVAGVKKLIMMTPAGKDGTVQPCTLVAAKIAGVTE
                     IYKVGGAQAIAALAFGTQSIPRVDKIVGPGNIFVALAKKAVFGYVGIDSVAGPSEILV
                     IADETANPRYVAADLLSQAEHDQMASAILITTSETFARQVSDEAARFTETLSRKDIIE
                     RSLDNYGYILLAASMDEAVDAANEIASEHVEIITKDSFSLMTKIKNAGAIFIGENSSE
                     PLGDYFAGPDHVLPTNGTARFFSALGVDAFYKRTSIISYSREALAPVAEDIAIFAEAE
                     GLTAHANSIRVRFE"
     gene            885..1481
                     /gene="hisB"
                     /locus_tag="PLU43_03865"
     CDS             885..1481
                     /gene="hisB"
                     /locus_tag="PLU43_03865"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOO27577.1"
                     /translation="MKTERKAEIERNTKETQIKLLLNLDGSGKSKIDTGIGFFDHMLD
                     GFTRHGFFDCELSVTGDLHVDGHHTVEDTGIVLGTAIKEALSDKKGIRRYGSMILPMD
                     DALVLCAVDLCGRPYFSYEASFETERIGYLDTELIREFFYAVSYSAAMNLHIKVLSGC
                     NSHHIAEAMFKAFAKALDEAVSMDARIKDVLSTKGTLA"
     gene            1478..2752
                     /gene="hisIE"
                     /locus_tag="PLU43_03870"
     CDS             1478..2752
                     /gene="hisIE"
                     /locus_tag="PLU43_03870"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOO27578.1"
                     /translation="MNNKKLIPCFYLKNGQLIKGFKDNEQISDDPVSYAVKCSNQGAD
                     ELILFDLSEGDEEHEEALLLIRAISRAVDIPIIGAGNIKRTEDVKKLIYAGCKYAALN
                     MAKQANIDLMEEVSKRFGREKIMACADAEEQILNSFENLKKYTSGVLLLNKHIVSGYK
                     DLPLIPLINNSTEEELFALLKKKDIAGISGDMINSRMDDLMKFKRSCKDNGILVNTFE
                     SCFSWDDFKKNSDGHITVVVQDYKTDAVLMVAYMDQEAYEKTIETGIMTYYSRSRNEL
                     WVKGATSGHYQYVRSLTADCDLDTLLAKVAQTGAACHTGRYSCFFNEIIQKDFTDNHP
                     LQVFENVYSVIMDKKEHPKEGSYTNYLFDKGMDKILKKIGEEATEIVIAAKNPDKEEV
                     KYEIADFLYHAMVLMAECDLTWTDITRELANR"
     gene            2837..>3189
                     /locus_tag="PLU43_03875"
     CDS             2837..>3189
                     /locus_tag="PLU43_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27579.1"
                     /translation="MKGIIKKTLLTAAAALFTCIFFINVNAEAKTTHNVAFIYGTKIN
                     MQTVTDGGNAVIPTDTSVSGYTFIGWTDSAVNIQSDKIILGMYTNNTPYAASTSAVST
                     VKKINDNTTAPFLPW"
BASE COUNT          966 a          605 c          773 g          845 t
ORIGIN      
        1 cttccacgct gatgtgtgtt cttccggcga aagtcgcagg cgtgaagaag ctcatcatga
       61 tgacgcctgc cggaaaagac ggaaccgttc agccgtgtac gcttgtcgct gcaaaaattg
      121 caggtgtaac tgaaatctat aaggtgggcg gagcccaggc gatcgcagcg cttgcttttg
      181 gcactcagtc catcccgcgg gtagacaaga tcgtcggacc tggaaatatc tttgtggcgc
      241 ttgccaaaaa ggcggtattt ggatatgtcg gtattgattc tgttgccgga ccgagtgaaa
      301 tactcgtcat tgcggacgag actgctaatc caaggtatgt ggcggctgat cttttgtcgc
      361 aggcagagca tgaccaaatg gcatccgcta ttttgattac gacttctgaa acatttgcca
      421 gacaggtgtc ggatgaggct gcccggttca cggaaacact gtcgcgcaag gatattattg
      481 aacgttccct tgacaattat ggatatatcc tgcttgccgc ttctatggac gaagcggtcg
      541 atgctgcaaa cgaaattgct tctgagcatg tggagatcat tacaaaggat tcgttttccc
      601 tgatgacgaa aatcaaaaat gcaggtgcga tctttatcgg agagaattct tcggaaccgc
      661 ttggcgatta ctttgcaggt cctgatcatg tactgccgac aaatggcacg gccagatttt
      721 tttctgcact tggtgttgat gctttttaca agagaacaag cattatttcc tactcaaggg
      781 aagcacttgc accggtagca gaggacattg cgatttttgc cgaggcagaa ggactgacag
      841 cgcatgccaa ttcaatacgt gtacgctttg aataaggagg tattatgaaa actgaaagaa
      901 aagctgaaat tgaacgaaat acaaaagaga cccagataaa gcttctgctg aatttggatg
      961 ggagcggaaa aagtaagatt gacaccggca tcggattttt cgatcatatg ctggatggct
     1021 ttacacgtca tggttttttt gactgcgagc tttcagttac cggagacctg catgtggacg
     1081 gacaccatac ggtggaggat accggcattg tgcttggtac ggccattaaa gaagcacttt
     1141 ccgataagaa aggaatcagg cgctatggaa gtatgatcct tccaatggat gatgcgcttg
     1201 tattatgtgc cgttgatcta tgcggccgtc catatttttc ttatgaagct tcttttgaaa
     1261 cggaaaggat cggatatctg gatacggaac tgatcaggga attcttttat gcggtcagtt
     1321 attctgccgc aatgaatctg catataaaag ttctttccgg ctgtaattcg catcatattg
     1381 ccgaagcaat gttcaaagct tttgcaaaag cattggatga ggcagtatcc atggatgcac
     1441 ggatcaaaga cgtattatct acgaaaggaa ccctggcatg aacaataaaa aactgatccc
     1501 ctgtttttat ttaaaaaatg gtcaactgat caaaggcttt aaagacaatg aacaaatttc
     1561 agacgatcct gtttcctatg cggtcaaatg cagtaatcag ggtgcagatg aactgatcct
     1621 ttttgactta tccgaaggag atgaagaaca tgaggaagcg cttcttctga ttcgtgcgat
     1681 cagcagagct gtcgatattc cgattatcgg ggccggaaat atcaagcgta cggaagatgt
     1741 caagaaactg atctatgcag gctgtaaata tgcggctctc aatatggcaa aacaggccaa
     1801 tatcgatctg atggaagaag tcagcaagcg atttggcagg gagaagatca tggcatgtgc
     1861 agatgcggaa gaacagatcc tcaattcctt tgaaaattta aaaaaatata caagcggcgt
     1921 attgctgctg aataaacata ttgtcagcgg ttataaagat ctgccgttga ttcctttgat
     1981 caacaattcc acggaagagg agctttttgc tcttttaaaa aagaaagata tcgccggaat
     2041 atcaggggat atgatcaaca gcagaatgga tgatcttatg aagttcaaga gatcatgcaa
     2101 agataacgga attttggtca atacctttga aagctgtttt tcatgggatg attttaaaaa
     2161 gaacagtgac gggcatatta ctgtagttgt acaggattat aaaacagatg ctgtgctgat
     2221 ggtcgcatac atggaccagg aggcttatga gaaaacgatc gaaaccggca ttatgaccta
     2281 ttattccaga agccgtaatg aattatgggt gaagggcgca acaagcgggc attaccagta
     2341 tgtcagaagc ctgaccgcag actgcgatct ggatacgctg cttgcaaagg tcgcgcaaac
     2401 aggcgctgcc tgccataccg gcagatattc ctgttttttt aatgaaatta ttcagaaaga
     2461 ttttacggac aatcatcccc ttcaggtgtt tgaaaatgtc tattctgtaa ttatggacaa
     2521 aaaagagcat ccaaaagaag gttcctatac aaactatctg tttgataagg gaatggacaa
     2581 aatattgaaa aagatcggag aagaagcgac agagatcgtc attgccgcta aaaatccgga
     2641 caaagaagaa gtcaagtatg aaattgcaga ctttttatat catgcaatgg tactgatggc
     2701 ggaatgtgac ctgacctgga cggacattac aagggaactg gcaaacagat aaaaactact
     2761 ggatttttat ctgtgaaaag ggtatactgg taatatgagt tgttgtataa gattaagggg
     2821 acgaggaggt ttttgtatga agggaatcat taaaaaaaca ttattaactg cggcagcggc
     2881 actatttaca tgtatttttt tcatcaatgt aaatgcggaa gcaaaaacaa cacataacgt
     2941 cgctttcatc tatggaacaa agatcaacat gcagacagtt accgatggcg ggaatgcggt
     3001 gattccgaca gacacgtctg tcagcggtta tacttttatc ggctggacgg acagtgctgt
     3061 taatattcaa agcgataaga tcattcttgg catgtataca aataatacgc cttatgcagc
     3121 atctacaagt gctgtatcga ccgtaaaaaa aataaacgat aacacaactg ctccttttct
     3181 tccatggtg
//
LOCUS       DAOSYE010000166         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22270_length_3171_cov_2.948652, whole genome shotgun sequence.
ACCESSION   DAOSYE010000166 DAOSYE010000000
VERSION     DAOSYE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22270_length_3171_cov_2.948652"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLU43_03880"
     CDS             <1..196
                     /locus_tag="PLU43_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO27580.1"
                     /translation="VVSIKEFGAFLEFAPGKEGMVHISKISKTRINRVEDVLTLGEVV
                     TVKCLGKDKMGRISFSMKDV"
     gene            complement(301..543)
                     /gene="grcA3"
                     /locus_tag="PLU43_03885"
     CDS             complement(301..543)
                     /gene="grcA3"
                     /locus_tag="PLU43_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA3"
                     /protein_id="HOO27581.1"
                     /translation="MENNHTQINNLVTLLDGYTEKGGHHLNINVLNRETLLDAQKHPE
                     KYPQLTVRVSGYAVNFVKLTPEQQNDVISRTFHEEM"
     gene            1257..2414
                     /locus_tag="PLU43_03890"
     CDS             1257..2414
                     /locus_tag="PLU43_03890"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOO27582.1"
                     /translation="MNRKRVILIVMDSFGIGEMPDAALYGDAGTNTLRSCTTSSYFKA
                     ENLKKLGLFNIDGVDFAEKEKEPVGSYARMTEKSKGKDTTIGHWEIAGLVSDEPLPTY
                     PHGFPKEVLDEFSKKTGRGVLCNLPYSGTDVIRDYGDEHVRTGDLIVYTSADSVFQIA
                     AHEEVVPLETLYEYCRIAREILKGRHGVGRVIARPFIGENGNYTRTSNRHDFSLLPPG
                     DTMLDHLKAAGLDVIGVGKIKDIFAGKGITEYTYTTGNPDGIEKTLEYMDTDFHGLLF
                     VNLVDYDMLYGHRNNIDGYAKAVAYFDEKLVQIMDRLREEDLLMITADHGCDPGYTVS
                     TDHSREYTPFLLYAKSMEAKNYGTRESFADIGATVLDYLGVSGTIYGKSVL"
     gene            2435..>3171
                     /locus_tag="PLU43_03895"
     CDS             2435..>3171
                     /locus_tag="PLU43_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO27583.1"
                     /translation="MSDYIEEINKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSV
                     KGKATARHAVSDWMEIEKERGISVTSSVLQFQYDGFCVNILDTPGHQDFSEDTYRTLM
                     AADSAVMVIDAGKGVEAQTRKLFKVCVMRKIPIFTFINKMDRDANDTFELLDEIEKEL
                     GIATCPMNWPIGSGKRFKGVYDRQKKCVVTFSDTEKGTKEGHFELIPVTEKEKLKACI
                     TNDAQEQLEEEIELLDGASAEFDLEEV"
BASE COUNT          986 a          510 c          806 g          869 t
ORIGIN      
        1 agtggtcagc attaaagaat ttggagcatt tttggagttt gcaccaggca aagagggtat
       61 ggttcatatt tcgaagatct ccaaaaccag aatcaaccgt gtggaagatg tgcttacgct
      121 tggagaggtt gttacggtaa aatgtcttgg aaaagataaa atgggaagga tcagcttctc
      181 catgaaagac gtataagcgg cgtttagctg tatgataaag aaaacaccgg atgaaaattg
      241 cttttcatcc ggcgtttttt ctttatcaca ggaagaatcc aatcctgcgt tcagacgatg
      301 ttacatttct tcatgaaagg ttctggaaat cacatcgttc tgctgttcgg gtgtcagttt
      361 gacaaagttg acggcatatc cggaaacccg gacggttaat tgcggatatt tttccgggtg
      421 cttctgtgca tccaaaagtg tttctctgtt caatacgtta atatttaaat ggtgtccgcc
      481 cttttccgta tatccatcta aaagagtgac gagattattt atctgtgtgt gattattttc
      541 cataaagtcc cctccttaca aaatgataat cattactgtt attttcaata aaactataac
      601 attgaataaa tgaaatgtaa acagtttttc agaaactttt tttttaaagc agttgttatt
      661 tgttgacaaa ttttcagaaa attcagatga attctattgt taaaaaaaat taaatgataa
      721 agaaatactt tttgtaaaat atttatattt attgaatcta tcaactttat ccacatttaa
      781 ataaaaatta aatcattatt aaataatata gaaataacat ataaaatctg aaaaatgcag
      841 taaaatcaag agatatggag atgtggaaac tgtggattat gagtgtatca tgaaaactgt
      901 tattgaaatt ggtcgaactg tggataagtc tgttaatatt gtatggaatg acggtattgt
      961 taaacaaaaa tgttaaaatt cagaaaattt acgagcagaa tatcagctat aaaaaacagg
     1021 cgctatgtgt ttatttgcag cctggtatat gatttttaaa ggtgtccggc tgctatgtgt
     1081 ataataagcg gagagaataa atttttatgg aaaataacat ggtttcgtgc tacaataaac
     1141 tggcactggc ataacaagtg ccaatttatt ccatgttgtg atagatgtgg tatttgacag
     1201 aatctatgga gctttatcag gaagcggaaa acagacgaat gacaggaggt gttttgatga
     1261 atcgtaaacg tgttatattg atcgtaatgg acagttttgg aatcggtgaa atgccggatg
     1321 cagctcttta tggagacgca ggaacaaata cactgcgatc ctgcacgaca agcagttatt
     1381 ttaaggcgga aaatttaaaa aagctggggc tttttaatat tgacggcgtg gattttgcgg
     1441 aaaaggagaa ggagccggtc ggaagctacg caaggatgac ggaaaaatcc aaaggaaagg
     1501 atacgacgat cggacactgg gagatcgcag gactggtttc cgatgaaccg ctgccgactt
     1561 atccgcatgg gtttccaaaa gaagtgctgg atgagttttc aaaaaagaca gggagaggag
     1621 tgctttgcaa tctgccctat tccggtacgg atgtcatcag ggattatgga gacgagcatg
     1681 tcaggacagg agatctgatc gtgtatacgt ctgcggacag tgtgtttcag atcgccgcgc
     1741 atgaggaggt cgtaccgctt gagacgctgt atgaatactg ccggatcgcg cgggagatcc
     1801 taaagggtag acacggtgta ggccgggtga ttgcaaggcc ttttatcgga gaaaacggaa
     1861 attatacaag aacttcgaac cggcatgatt tttcattgct gccgccaggg gatacgatgc
     1921 ttgatcatct gaaagccgca ggtcttgacg tgatcggagt aggtaagatc aaggatattt
     1981 ttgcgggaaa aggaattacg gagtatacgt atacaacggg taatccggat ggcatagaaa
     2041 aaacactgga atatatggat acggattttc atggactttt atttgtaaat ctggtagatt
     2101 atgatatgct gtacggacac cgtaataata ttgacggcta tgcaaaggca gtcgcatatt
     2161 ttgatgaaaa actcgtacag atcatggaca ggcttcgtga ggaagatctt ctcatgatca
     2221 cggctgatca cggctgcgat ccgggatata ccgtatctac ggatcattca agggagtata
     2281 cgccattttt gctgtatgcg aaatccatgg aagcaaagaa ctacggaaca agagaaagct
     2341 ttgcggatat tggcgcaaca gttctggatt accttggagt gagcgggacg atttatggaa
     2401 aaagtgtatt ataatattgt ttttggaggc tgacatgtcg gattatatag aagaaatcaa
     2461 caaaagaagg acctttgcga tcatttcgca cccggacgcg gggaagacga cactgacgga
     2521 gaaatttctt ttgtatggcg gtgcaatcaa tctggcaggc agtgtaaaag ggaaagcaac
     2581 ggcaagacat gcggtatcgg actggatgga aattgaaaaa gagagaggta tttccgttac
     2641 gtcaagtgta ctgcagtttc agtatgacgg attttgcgtg aatattctgg atacgccggg
     2701 acatcaggat ttttcagagg atacgtaccg gacgctgatg gcagcggatt ccgcggtcat
     2761 ggtcatcgat gccggcaagg gtgtcgaagc gcagacgcga aagctgttca aagtatgtgt
     2821 tatgcgtaaa attccgatct ttacctttat caataagatg gaccgggatg caaacgatac
     2881 gtttgaactg cttgatgaaa tcgaaaagga gctcgggatc gcaacctgtc cgatgaattg
     2941 gccgatcggt tccggcaagc gttttaaggg tgtgtatgac cgacagaaga aatgtgtcgt
     3001 gacattttcc gatacggaaa agggaacgaa ggaaggacat tttgaactga ttccggtcac
     3061 ggaaaaggaa aagctgaaag cgtgtattac gaatgatgcg caggaacagc tggaggaaga
     3121 gatcgagctg ctggacgggg ccagtgcgga gtttgatctg gaagaggtaa g
//
LOCUS       DAOSYE010000167         3170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22282_length_3170_cov_3.693419, whole genome shotgun sequence.
ACCESSION   DAOSYE010000167 DAOSYE010000000
VERSION     DAOSYE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22282_length_3170_cov_3.693419"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..505)
                     /gene="atpC"
                     /locus_tag="PLU43_03900"
     CDS             complement(95..505)
                     /gene="atpC"
                     /locus_tag="PLU43_03900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOO27584.1"
                     /translation="MAEDRAFKLRIISPDRLFYEGDVIMVEVRTTEGEIGVLKHHIPL
                     TAVLAPGVARIKESETEIKIAALHGGFIEILQDSMTILAEIVEWPEEIDLHRAQEARV
                     RAERRLSEKATGTNMARASLALQKSITRIEAAKK"
     gene            complement(517..1917)
                     /gene="atpD"
                     /locus_tag="PLU43_03905"
     CDS             complement(517..1917)
                     /gene="atpD"
                     /locus_tag="PLU43_03905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOO27585.1"
                     /translation="MADKNVGKITQIVGAVLDVKFSEESLPQINEAIKVLKKDNTELV
                     VEVAQHLGDHTARCIAMGPTDGLVRGMEAVATGSPISVPVGEKTLGRIFNVLGQPIDN
                     KPAPEGVDYFPIHRKAPAFEEQSTSAELLETGIKVVDLLCPYQKGGKIGLFGGAGVGK
                     TVLIQELIRNIATEHGGYSVFTGVGERTREGNDLYYEMKESGVIDKTTMVFGQMNEPP
                     GARMRVGLSGLTMAEYFRDQGGKDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPT
                     LQTEMGALQERITSTKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSIVEL
                     GIYPAVDPLESTSRILDPRIVGEEHYRVARGVQEILQKYKELQDIIAILGMDELSEED
                     KVTVSRARKVQRFLSQPFFVAVQFTGCDGRYVPIADTIQGFKEILEGKHDGIPESYFL
                     NAGSIEDVLAKVDANK"
     gene            complement(1941..2813)
                     /gene="atpG"
                     /locus_tag="PLU43_03910"
     CDS             complement(1941..2813)
                     /gene="atpG"
                     /locus_tag="PLU43_03910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOO27586.1"
                     /translation="MASMRDIKRRKVSIQSTQQITKAMKLVSTVKLQRAKTKAERTKL
                     YFDCMYETVTSMLEKSGYMDHPYMKPNDSSKKVVIAITSNRGLAGGYNSNITKLITGS
                     GWEKEDVEIYAVGKKGRDILKRRGYQIRKDYPDLINAPIFSDAAEISEAVLQSFSKGE
                     IGEIYLAYTAFKNTVSHIPTLLKLLPIAFEADTKRPEEKSVNDFVLMNYEPEEKEALD
                     MIIPKYVTSLIYGALIEAVASENAARMQAMDSATSNAEDMIVNLELKYNRARQGHITQ
                     ELTEIIGGAEALNA"
     gene            complement(2833..>3170)
                     /locus_tag="PLU43_03915"
     CDS             complement(2833..>3170)
                     /locus_tag="PLU43_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27587.1"
                     /translation="KAFKAIFFGGSDTKEKLAQGERIREVLKQPQYQPMPVEYQVIMI
                     YVVTRKYLLDVPVDRIMDLEKELLEYIKTNYSEIPSSIKETKMISEEMEEKLKKAIMD
                     CKEEFLNQA"
BASE COUNT          766 a          845 c          598 g          961 t
ORIGIN      
        1 tatactttca gcaatgctag caaaaccaag gtctacgaat tatgaaattt cttcaattag
       61 attttccccc tctgttgaga aaatttttat attgttattt tttcgcggcc tcaatacgcg
      121 taatagactt ttgcagtgca aggctggccc ttgccatatt cgtgccggtc gccttttcag
      181 aaagccttct ctccgcacgg acccttgctt cctgtgcacg gtgaagatcg atctcttccg
      241 gccactcaac gatttccgca aggatcgtca tactgtcctg caaaatttca ataaagccgc
      301 cgtgcaatgc cgcaattttt atctcggttt cagattcctt tattctggca acaccgggcg
      361 ccaaaacagc agtcagcgga atatggtgct ttaacacacc gatctcccct tctgtcgtcc
      421 ggacttcaac catgatcacg tctccctcgt aaaacagtct gtccggagaa ataatcctca
      481 gtttaaatgc tctgtcttct gccatattca agctccttac ttatttgcat ccacttttgc
      541 cagtacatcc tcaatactgc cggcattcag gaaataactc tcaggaatac catcgtgttt
      601 accctcaagg atttccttga acccctggat cgtatctgcg atcggaacat aacgaccgtc
      661 acagcccgta aactgcaccg caacgaaaaa cggctgtgac agaaaacgct gtacttttct
      721 cgctctcgac actgtcacct tatcttcctc ggaaagctcg tccataccga gaatcgcaat
      781 gatatcctgc agttccttat atttctgcaa aatctcctgc acaccgcgtg ctacccggta
      841 atgctcttct cccactatac gcggatcaag gatacgcgaa gtagattcaa gcggatcgac
      901 cgccggataa ataccaagct ctacgataga acgggaaaga accgttgtcg catccagatg
      961 tgcgaacgta gtcgccggtg ccggatcagt caggtcgtct gcaggtacat aaacagcctg
     1021 tacagatgtg atagaaccgt tctttgtaga agtaatacgc tcctgaagcg cacccatttc
     1081 cgtctgcagc gtcggctgat aacctacggc cgacggcatg cgtccaagaa gtgcggacac
     1141 ttcggagccg gcctgtgtga aacggaaaat attgtcaatg aataaaagca catcctttcc
     1201 gccctgatcc ctgaaatact ctgccatcgt aaggcccgaa agtccaactc tcatcctggc
     1261 tcccggcggt tcattcatct gtccgaatac catcgttgtc ttgtcgataa cgccggattc
     1321 tttcatttca taatatagat cgtttccttc acgggttctt tcgccaacac cggtaaagac
     1381 ggaatatccg ccgtgctcgg tcgcaatatt tctgatcagt tcctgaatca atacggtttt
     1441 tcctacacca gcaccgccga acaggccgat cttaccgccc ttctgataag gacaaagaag
     1501 gtccacgact ttgatgcccg tctcgagcag ttccgccgaa gtggactgtt cttcaaaagc
     1561 cggagctttt ctgtgaatag gaaaatagtc tactccttcc ggcgccggtt tattgtcaat
     1621 aggctggccc aaaacattga aaatacggcc aagcgtcttt tcaccgaccg gcacggatat
     1681 cggagagccg gttgcaaccg cttccattcc cctgacaaga ccgtctgtcg gacccatggc
     1741 aatacatctt gccgtatgat cacccaaatg ctgcgcaacc tctacaacca attccgtatt
     1801 gtcttttttc agcactttga tcgcttcatt gatctgcgga agcgattctt cggaaaattt
     1861 tacatccagc accgcaccga caatctgggt aatcttacct acgtttttat ctgccactat
     1921 tttgttcact ccttttcgtt ttatgcgttt aatgcctctg ccccgccgat gatctccgtc
     1981 agctcctgtg taatatgacc ctgtcttgcc ctgttatact taagttccag attcacgatc
     2041 atatcttcgg cattacttgt agcggaatcc attgcctgca ttctggcggc attttcactc
     2101 gctacggctt ctatcagcgc gccgtatatc aggctggtca catatttggg aataatcata
     2161 tccaaagctt ctttttcttc cggttcataa ttcatgagca caaaatcatt tacggatttc
     2221 tcttccggtc ttttcgtatc agcttcaaat gcgatcggaa gaagtttgag cagagtcggt
     2281 atatgactga ccgtgttttt aaatgccgta tatgccagat aaatttctcc tatttctcct
     2341 tttgaaaaag actgcagtac ggcttcgctg atttctgcag catcggaaaa aataggtgca
     2401 ttgatcagat ccggataatc tttccgaatc tgataacctc gtctttttaa aatatcccgg
     2461 ccttttttac cgaccgcata tatttcaaca tcctcttttt cccatccgct ccctgtaatc
     2521 agctttgtaa tattggaatt ataaccgcca gccagacctc tgttggaagt aatggcaatt
     2581 acaacttttt tagaggaatc gttcggtttc atatacggat ggtccatata tcctgatttc
     2641 tccagcatgg aagttactgt ttcatacatg cagtcaaaat ataatttcgt acgttccgcc
     2701 tttgttttcg ctctctgcaa tttcacagta gaaacaagct tcatggcttt tgtgatctgc
     2761 tgcgtacttt gaatcgacac tttgcgtcgc ttaatatctc tcattgaggc catgctttat
     2821 caccttacct gtctatgcct gatttaaaaa ctcctcttta caatccataa ttgctttttt
     2881 gagcttctct tccatttcct cagaaatcat tttagtctct ttaatggaag atggtatttc
     2941 cgaataattt gtttttatat attccagcag ttccttttca agatccatga tcctgtctac
     3001 cggaacatcc agcagatatt tcctggttac aacataaatc ataatgacct ggtattctac
     3061 cggcatcggc tggtactgag gctgcttcag gacttccctg atcctctctc cctgtgccag
     3121 tttttccttt gtatccgaac ctccgaaaaa tattgctttg aatgcttttc
//
LOCUS       DAOSYE010000168         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22329_length_3167_cov_2.627249, whole genome shotgun sequence.
ACCESSION   DAOSYE010000168 DAOSYE010000000
VERSION     DAOSYE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22329_length_3167_cov_2.627249"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLU43_03920"
     CDS             <1..376
                     /locus_tag="PLU43_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO27588.1"
                     /translation="RCDGSGYPQKLHGESINPFAKIISILDAYEAMASYRSYRPPLCP
                     FTVIDIFEKTGLEKYDVEYYLVFLERMVEEYIGKEVMLNDGTECIVVLIHKHALSRPM
                     VKADDKYIDLSTNKNLFIETLI"
     gene            complement(442..891)
                     /locus_tag="PLU43_03925"
     CDS             complement(442..891)
                     /locus_tag="PLU43_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO27589.1"
                     /translation="MIEATSCGGVVIFRGKILLLYKNFKNKYEGWVLPKGTVEEKEDF
                     KDTALREVLEETGVQASIIKYIGMSQYSFNVSKDTVEKDVHWYLMMADSYYSKPQREE
                     YFVDSGYYKYHEACHLLKFANERQILEKGYEEYLNLKKNNLWGGNNR"
     gene            complement(1024..2430)
                     /locus_tag="PLU43_03930"
     CDS             complement(1024..2430)
                     /locus_tag="PLU43_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO27590.1"
                     /translation="MLHKKNPKKENIKQMDNKNENREIGNITYLFVGLFIVLIGYLMY
                     FTLFVSSGFINSSYNSRPELLAQKIVRGSILSRNGEVLAKTETASDGTESRIYPYANL
                     FAHAVGYSTHGKTGVESIADFTLLTSNAFIGDRIENDIQGVKNQGDTVITTLDVNIQK
                     TAYDAMGVYQGAIVVMNVKTGEILALLSKPDFDPNQINQIWDEINADSDSSVLLNRAT
                     QGLYPPGSTFKIVTALEYLKENDDDVSSYSYDCTGSFQTGDSVISCYHGQSHGHVNFT
                     ESFAKSCNSSFANIGMTLDKKEFQKTCEALLFNTELPVPYSYKESYVPITASSDTDEL
                     LQTAIGQGKTQVTPIHMALITSAIANDGILMKPYVISSVENADQDIVKSYKSQEYKRL
                     LDEDDAEELQELMAEVVQNGTATKLKDTNGYIAAGKTGSAEYSLVKSESHAWFTGFAP
                     TSDPEIANCNFGRCRFRR"
     gene            complement(2434..>3167)
                     /locus_tag="PLU43_03935"
     CDS             complement(2434..>3167)
                     /locus_tag="PLU43_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOO27591.1"
                     /translation="FAIAGILTKSTDLRQIMLSAAVAGLHVIILVLSKDLGGAVIFFV
                     VYFSMLYVATQNPGYCLFGVLSGAAAAVIGYKLFAHVRIRVQAWKDPLGTINDAGYQI
                     AQSLFAIGTGSWFGMGLGQGAPSNIPVVESDFIFSAITEEFGVIFGMCLILICVSCFV
                     MFMNISMRFHNTFYKLVAAGLAIVYGFQVFLTIGGVTKFIPLTGVTLPLVSYGGTSVI
                     VSLIMFAIIQGLYITVRDEAAKKDG"
BASE COUNT          911 a          738 c          582 g          936 t
ORIGIN      
        1 gcgctgtgac gggagcggct atccgcaaaa actccatgga gaaagcatta atccatttgc
       61 caagatcatt tccattctcg atgcctatga ggctatggcg tcttatcgtt cttaccgtcc
      121 tccgctttgt ccgttcacag tcattgatat tttcgaaaaa acaggtcttg aaaaatatga
      181 tgtcgaatat tatctggtgt ttttggaaag gatggtcgag gaatatatcg ggaaggaagt
      241 catgctcaat gacggtacgg aatgcattgt tgtactgatc cacaaacatg cactgtcacg
      301 cccaatggtg aaagcagatg ataaatatat tgatctttct acaaataaaa atttatttat
      361 tgaaacattg atctgaatat aaaaaacaaa tgtgttttaa tatacgagag ccgcgcagaa
      421 cgagcggctc tcgtatattg ttcagcggtt attcccgccc cacaaattat tttttttcaa
      481 attcaaatat tcttcatatc ccttctctaa tatctgccgt tcatttgcaa atttcaacag
      541 atgacaggct tcatgatatt tataatagcc ggaatcgaca aaatattctt cacgctgtgg
      601 tttactgtaa taactgtcgg ccatcatcag ataccagtgc acgtcttttt ccacggtatc
      661 ctttgagacg ttaaacgaat actggctcat tccgatatat ttgataatcg atgcctgtac
      721 tccggtttct tccagtactt cccgtaaagc agtatcttta aaatcttctt tctcttctac
      781 agttcctttc ggcaataccc agccttcgta tttattttta aaatttttat acaaaagcaa
      841 aatcttaccc cgaaaaatca ccacaccgcc acagcttgtt gcttcaatca ttgcaattcc
      901 tcctgttctg gtgttatagc tgcctgcttg attatatcaa taccaatact tttataaccg
      961 tagctagtat accatcaatt gaaataggcg tcaaatatcc gctttgcgat cggaacagca
     1021 tattcaccgc cggaaccggc accttccaaa attacagtta gctatttcag gatccgacgt
     1081 cggggcaaag cctgtaaacc atgcatggct ttcagattta accagactgt attccgcaga
     1141 acccgtcttt cctgcagcaa tgtaaccgtt cgtatctttt aattttgtag cagtcccatt
     1201 ctgtacaact tcagccataa gttcctgcag ttcttctgca tcatcttcat ccagcagacg
     1261 tttgtattcc tgggatttat atgattttac aatatcctga tctgcatttt ccacacttga
     1321 gattacatac ggtttcatca gaattccgtc atttgcgatc gcggatgtaa taagcgccat
     1381 atgaatcggc gtgacctgtg ttttcccctg accgatcgca gtctgcaaaa gttcgtccgt
     1441 atcggaggaa gctgtgatcg gtacatagct ctctttatac gagtaaggaa ccggaagctc
     1501 cgtattgaat aaaagtgctt cacatgtctt ttgaaattcc tttttatcaa gcgtcatccc
     1561 gatatttgca aatgaagaat tacaggattt tgcaaacgac tctgtaaagt tcacatgacc
     1621 atgactttgc ccgtgatagc agctgatcac ggaatcaccg gtctgaaaac ttcccgtaca
     1681 gtcataggaa taggaactga catcatcgtc attttctttt agatattcaa gcgccgtaac
     1741 gattttaaat gtcgaacccg gcggatataa accctgtgtc gcacgattca aaagaacact
     1801 gctgtcggaa tcagcattga tttcatccca tatctggttt atctgattcg ggtcaaaatc
     1861 cggttttgac aataacgcca gtatttctcc tgtttttaca ttcattacaa cgattgcccc
     1921 ctgataaacg cccattgcat cataggcggt tttttgaata ttcacatcta atgttgtaat
     1981 tacggtatct ccctggtttt ttactccctg aatatcattt tcaatacgat ctcctatgaa
     2041 tgcatttgaa gtgagcaggg tgaaatcagc aatggattcc acacctgttt ttccatgtgt
     2101 agaataaccg acagcatggg caaacagatt tgcatacgga tatatccgtg attctgtccc
     2161 atccgatgcg gtctctgttt ttgccaggac ctctccatta cgggacaaaa tactgccccg
     2221 cactatcttc tgggccagca gttccggtct ggaattatag gaactgttaa taaacccact
     2281 gctcacaaac agcgtaaaat acatgagata gccgatcaat acaataaata accctacaaa
     2341 aagataggtg atattcccga tctctctgtt ttcattttta ttgtccattt gctttatatt
     2401 ttcttttttg gggttctttt tatgtaacat gctttatccg tccttttttg cagcttcgtc
     2461 cctgaccgta atataaagac cctgtatgat cgcaaacatg atcagggata caataacgga
     2521 agttcctcca taactgacaa gaggcagtgt cacaccggtc aatgggatga atttggtgac
     2581 tccgccgatt gttaagaata cctgaaatcc atatacgatc gcaagaccgg ctgccacaag
     2641 tttataaaat gtattatgaa aacgcatgga aatgttcatg aacatgacaa aacaactcac
     2701 gcatatcaga atcaggcaca taccgaaaat cacaccaaac tcttcggtaa tagccgagaa
     2761 aataaaatcc gattccacaa caggaatgtt ggatggcgca ccctgaccga gtcccatacc
     2821 aaaccagctt ccggttccga tcgcaaataa cgactgtgcg atctgataac cggcgtcatt
     2881 gatcgtgcca agcggatcct tccatgcctg taccctgatc cgcacgtggg caaacagttt
     2941 atatccgatc acggctgcgg cagctcccga caaaacccca aacaaacaat agccgggatt
     3001 ctgtgtggca acataaagca tggaaaaata tacgacaaag aaaataaccg caccgccaag
     3061 atcttttgac aaaacaagaa taataacatg aagtccggca accgctgcgc ttaacatgat
     3121 ctgtctaaga tccgtggatt ttgtcaaaat cccggcgatt gcaaata
//
LOCUS       DAOSYE010000169         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22338_length_3166_cov_3.315976, whole genome shotgun sequence.
ACCESSION   DAOSYE010000169 DAOSYE010000000
VERSION     DAOSYE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22338_length_3166_cov_3.315976"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLU43_03940"
     CDS             complement(<1..598)
                     /locus_tag="PLU43_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOO27592.1"
                     /translation="MKKTALIMAGGRGERFWPRSRKNLPKQFLSLTDDGKTMIQLTVE
                     RILPIVDIEDIYISTNKDYLPLVAAQFPEIPAENILCEPVGRNTAPCIALGAAHISRK
                     YDDAIMMVLPSDHLIKYSSMYQTTLRDACEVAMQDDNLVTIGITPDYPETGYGYIKFV
                     NEICEGRAFKVDKFVEKPTLELAKEYLAADEYLWNSGMF"
     gene            759..1715
                     /gene="prmA"
                     /locus_tag="PLU43_03945"
     CDS             759..1715
                     /gene="prmA"
                     /locus_tag="PLU43_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOO27593.1"
                     /translation="MKYNKYTVKTLTAAEDLVIGTLADIGIEGVEIEDKVPLTEMEKA
                     QMFVDILPEGPPDDGIAYISFYLDADTDNEPVLLAVRKELAELKTWTDPGDCTITVSQ
                     TQDEDFLNNWKQYFHQFYVDDILIIPSWEDVKPDDADKMVIHIDPGTAFGTGMHETTQ
                     LCIRQLRKYVTSDTALLDVGTGSGILSVMAYKFGAKSAIGTDLDICAVDAVKENMKAN
                     GLEDRGFRLLIGNIIDDRSIQDAVGYEAYDIVAANILAEVLVPLTPVIWNQLKPGGIY
                     ITSGIIDDKEEIVAAAVEAAGFTVLKICRQGEWVSVTARKPV"
     gene            1742..2488
                     /locus_tag="PLU43_03950"
     CDS             1742..2488
                     /locus_tag="PLU43_03950"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOO27594.1"
                     /translation="MYQFFAEKNQINGTDITITGEDVNHIKNVLRMKIGEELSVVTDG
                     EELEYRCEIAAFEENKVRCRLRFIKEEGVELPNRICLFQGLPKNDKMELIVQKAVELG
                     ACEVIPVQTKRTVIKLDAKKEQTRITRWNAIAEAAAKQAKRRVIPQVKPVMTMKEAVD
                     YCKEFEAKFIPYELSDKNDMDSTRALFKQIRPKQRIAVFIGPEGGFDESEISLAIENG
                     LIPITLGRRILRTETAGFTVLAWLVYELEK"
     gene            2528..>3166
                     /locus_tag="PLU43_03955"
     CDS             2528..>3166
                     /locus_tag="PLU43_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO27595.1"
                     /translation="MQAYLDNSATTRCFDDVRQLMGEIMNTAYGNPSSMHQIGINAET
                     YVREAKRIFAENLKVSEREIYFTSGGTESDNLAIIGCAEANKRTGMHLITTRIEHPAV
                     LEPMKYLEKQGFRVTYLHVDESGRVNPEELKNALCKDTILVSIMFVNNEIGALQPIEE
                     IGDLLKRYSPQTLFHVDAVQGYGKFAIHPKRMKIDLLSISGHKIHGPKGVGVL"
BASE COUNT          924 a          623 c          847 g          772 t
ORIGIN      
        1 aaaacatgcc gctgttccac aaatattcgt cggcagccag atattctttt gcgagctcaa
       61 gggtcggctt ttctacaaat ttatctactt tgaaagcacg gccttcgcag atctcgttaa
      121 caaatttgat gtaaccgtag cccgtttccg ggtaatcagg cgtaatgccg atcgtaacaa
      181 gattatcatc ctgcatggcg acttcacagg cgtcccgcag tgtggtctga tacatactgc
      241 tgtatttgat caggtgatcg gacggcagca ccatcatgat cgcatcgtca tattttctgg
      301 aaatatgagc tgcgccgagc gcaatgcacg gagcggtgtt tctgccaaca ggctcacata
      361 gaatattttc cgccggaatt tcaggaaact gtgctgcaac aaggggcaga tagtctttat
      421 ttgttgaaat atagatatct tcaatatcga cgatcggcag aatccgttcg acggtcagct
      481 gtatcattgt ctttccgtca tctgtcaggg acagaaactg tttcggaaga ttttttctgc
      541 tgcggggcca gaaccgctcg cctcttccgc ctgccataat caatgctgtc tttttcatag
      601 aattgcccgc cttttgcttt attctgtcat ataagtaaat tataatttat ttaaagggaa
      661 atagcaaatg atatactact ggtcgtatgg aaaaaatcat gctatactta aaaattgata
      721 gcagaccggc tatgcagacg aaagaacgga gaaatcatat gaaatataat aaatatacgg
      781 taaaaacgct tacggctgcg gaagatctcg tgatcggcac attggctgat attgggatcg
      841 aaggtgtgga gatcgaagac aaagttcctt tgactgaaat ggaaaaggca cagatgtttg
      901 tcgatattct gccggagggc ccgccggatg acggaatcgc atatatcagt ttttatcttg
      961 atgcggatac ggataatgag ccggtgcttc tggccgtccg aaaagagctt gccgagctga
     1021 aaacatggac ggatccaggg gattgtacga ttactgtttc acagacacag gatgaagatt
     1081 ttctgaataa ctggaagcag tattttcatc agttttatgt agatgatata ttgatcattc
     1141 cttcctggga ggatgtgaag ccggatgacg cggataaaat ggttattcat atcgatccgg
     1201 gaactgcgtt cggaacaggt atgcatgaaa cgacccagct gtgcatcagg cagctgcgca
     1261 aatatgtgac atcggatacg gcactgcttg atgttgggac aggaagcggg atcctttctg
     1321 ttatggcata taaattcggt gcaaaatcgg cgattggaac cgaccttgat atctgtgcgg
     1381 tcgatgcggt aaaagagaat atgaaagcaa acggactgga agacaggggt ttcaggcttt
     1441 tgatcggaaa tattattgat gacaggagca tacaggatgc tgtcggttat gaagcctatg
     1501 acattgtggc cgccaatatt ctggcagagg tgcttgtacc gctgacaccg gttatctgga
     1561 atcagctgaa gcccggcggt atttatatta catcagggat cattgacgac aaggaagaga
     1621 tcgttgcagc tgcagtggaa gcggcaggct ttacagtgct taagatctgc cgtcagggag
     1681 aatgggtcag cgtgaccgcg cgaaagccgg tatgactgaa agaaaataaa ggaaatggaa
     1741 aatgtatcaa ttttttgcag aaaaaaatca aataaacgga accgacatta cgatcacggg
     1801 agaggatgtc aatcatataa aaaatgttct tcgcatgaag atcggtgagg agctgtctgt
     1861 tgtgacagac ggcgaagagc tggaataccg ttgtgagatc gcggcatttg aagagaacaa
     1921 agtgcgctgc aggctgcggt tcataaaaga agagggtgtc gagctgccaa acaggatctg
     1981 cctgtttcag gggcttccga agaatgacaa gatggagctg atcgttcaga aggcggtcga
     2041 actcggagcc tgtgaagtga ttcccgtaca gacaaaacgc acagtcatta aactggatgc
     2101 gaaaaaggaa cagacccgga tcaccaggtg gaatgccatt gcagaagcag ctgccaaaca
     2161 ggcaaaacgc cgggtcatac cacaggtaaa gccggttatg acgatgaagg aagctgtcga
     2221 ttactgcaaa gagtttgagg ctaaattcat accctatgaa ttgtctgaca agaatgatat
     2281 ggacagcacc cgtgcgcttt ttaaacagat ccggccaaaa cagcggatcg cagtttttat
     2341 tgggccggaa gggggctttg acgaatctga gatcagcctt gcaatagaaa acggcctgat
     2401 accgatcaca cttgggcgca ggatactgcg gacagaaaca gccggtttta cggtattggc
     2461 ctggcttgta tacgagctgg aaaaatagtt ggttttaatg gaaaataaaa gaacggagca
     2521 gaagtatatg caggcatatt tggataattc cgcgacaacc cgctgctttg acgatgtcag
     2581 acagctgatg ggtgaaatta tgaataccgc atacggaaat ccttccagta tgcatcagat
     2641 cggaatcaat gcggaaacat acgtaagaga agcaaagagg atctttgcgg aaaatctgaa
     2701 ggtttcggaa agggaaattt atttcacatc cggcggtacg gaatcggata atctcgcgat
     2761 cataggatgc gcagaagcga ataagcgtac agggatgcac ctgatcacga cgaggatcga
     2821 acatccggca gtgctggagc cgatgaagta tttggagaag cagggattcc gggttactta
     2881 tctgcatgtg gacgaaagcg gcagggtgaa ccctgaggaa ttgaaaaacg cgctgtgtaa
     2941 agatacgatt cttgtttcca ttatgtttgt caacaatgag atcggagcgc tgcagccgat
     3001 agaggagatc ggagacctgc tgaaacggta tagtccgcag acgctgtttc atgtggatgc
     3061 ggtacaggga tacggtaaat ttgcgatcca tccgaaaaga atgaagatcg acctgctttc
     3121 gatcagcggg cataagatcc atggaccaaa gggagtcggt gtttta
//
LOCUS       DAOSYE010000170         3162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22366_length_3162_cov_3.802382, whole genome shotgun sequence.
ACCESSION   DAOSYE010000170 DAOSYE010000000
VERSION     DAOSYE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22366_length_3162_cov_3.802382"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLU43_03960"
     CDS             <1..323
                     /locus_tag="PLU43_03960"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOO27596.1"
                     /translation="ILIDIFQSNHEGAIIDKIQESYYDHTDGIIINPGAYSHYSYAIH
                     DALSSVQMPKVEVHISDISKRAEEWRHQSVTSRACNLLIKGHGLDGYLEAIDYFIELN
                     RKNS"
     gene            392..949
                     /gene="efp"
                     /locus_tag="PLU43_03965"
     CDS             392..949
                     /gene="efp"
                     /locus_tag="PLU43_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO27597.1"
                     /translation="MISAGDFRNGLTVEMDNGIFQIIEFQHVKPGKGAAFVRTKLKNI
                     INGGVVEKTFRPTEKFPQARIDRNDMQYLYSDGDLYHFMNAENFEQIALDADAIGDAL
                     KFVKENEMVKVCAHNGSVFAVEPPLFVELEITETEPGFKGDTATGATKPATVETGAVV
                     YVPLFVEQNDKIKIDTRTGEYLSRV"
     gene            1066..1980
                     /locus_tag="PLU43_03970"
     CDS             1066..1980
                     /locus_tag="PLU43_03970"
                     /inference="COORDINATES: protein motif:HMM:NF039828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HOO27598.1"
                     /translation="MEYQEFLEKVKNETGQYLGEEFTVAIHQVLKNNAVSLYGLVITE
                     KEQNISPTIYMEPFFRQLMEGADFEDIFKEILQVYEESRLKENMDVSFFTDYSQVREL
                     LFLKLINYAANTELLKLVPHTRFLDLAIVPYCFVKNIAVGEASILVYHSHMEMWKIEE
                     RELMLEAKKNTREIMGSEIMALKELVEEAGCCEELPAGKEGEETEMYVLSNRYKLNGA
                     ICMTYPDVLDAFSEKTGKNLYVLPSSIHEVILIPASGMYELDRLSEMVHEVNMTQVEQ
                     EELLSDHAYYYEKNIGFDKSFMNACARN"
     gene            2072..2144
                     /locus_tag="PLU43_03975"
     tRNA            2072..2144
                     /locus_tag="PLU43_03975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2105..2107,aa:Arg,seq:ccg)
     gene            2215..>3162
                     /locus_tag="PLU43_03980"
     CDS             2215..>3162
                     /locus_tag="PLU43_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO27599.1"
                     /translation="MAGNKVKEFIVKHYRYFIVGGLFVVLVVVLIILMVKNKDNTSES
                     DSDVDAVLAESNIDVPKDKYEVNAYPEVNTLLESYYNAMAAGDADTMASLSNTMTDEE
                     KIRIQVTAEYYEGFSNYQVYTKKGPEENSYIAFVTYDIMFTGATVSAPALASVYVCTN
                     DSGALYINKDNLTDEENAYFRAISVQDDIEELCDEVKVAYNKVLESDETLAALMPTLK
                     DTINQQVKDQLAAQQQAEAEAAAQAEAAAQAAEQEASATMVRATDVVNIRSSASTESD
                     VLGKAAVGDEYTRYEEMDNGWSKIDYNGQEAYIKSDYLEV"
BASE COUNT          972 a          509 c          804 g          877 t
ORIGIN      
        1 atattctcat agatattttt cagagtaacc atgaaggtgc cattattgat aaaatacagg
       61 aaagctatta tgaccatacg gatgggatca ttattaatcc cggtgcgtat tctcattaca
      121 gctatgcgat ccatgatgcg ctgtcttcgg ttcagatgcc gaaggtagaa gtccatattt
      181 cggatatttc aaaaagggcg gaggaatgga gacatcaatc tgtgacatcc agggcctgta
      241 atttgttgat aaaagggcat ggattagacg ggtatttgga agcaattgac tattttattg
      301 aattaaatag aaaaaacagt tgaaatatct gttcttttag tataaactga taaagaatta
      361 ttacggaatg tatttaagga ggaatatcgg aatgatatca gcaggcgatt ttaggaatgg
      421 cttaactgtt gagatggaca atggcatttt tcagattatt gagtttcagc atgtaaaacc
      481 cggaaaggga gcagcatttg tcagaacaaa gttaaagaat attattaatg gcggtgtggt
      541 tgaaaaaact ttcaggccga ctgaaaagtt tccacaggct cgtatcgata gaaatgatat
      601 gcagtattta tattcagacg gtgatttgta tcactttatg aatgcagaga attttgaaca
      661 gatcgcttta gacgcagatg caatcggcga tgcgttaaag tttgtcaaag agaatgagat
      721 ggttaaggta tgcgcacata acggcagtgt atttgcggtg gagcctccct tgtttgtaga
      781 gcttgagatc accgaaacag agcctggatt taaaggggat acggcgacag gcgctacaaa
      841 acctgcaaca gtggagacag gcgcggttgt ttacgtaccg ttgtttgttg agcagaatga
      901 caagatcaag atcgatacga gaaccggaga atatctttca agggtatagg acttcggagc
      961 acataatatt tgaatttcag gactttctgg catgccggag agtccttttt tctctccttc
     1021 agttaagcgg taactgtcaa ttggccaata tagaaggaga tttatatgga atatcaggaa
     1081 tttttagaga aagtgaaaaa tgaaacaggg cagtatcttg gagaggaatt tacggttgcg
     1141 atccatcagg tgctgaaaaa taatgcggtt agtctgtatg gacttgttat tacggaaaaa
     1201 gagcagaata tttcgccaac catttacatg gagccctttt tcagacagct tatggagggt
     1261 gccgattttg aagatatttt taaagagatc ctgcaggtat acgaagaaag ccgtttgaag
     1321 gaaaacatgg atgtttcttt ttttacggat tacagtcagg tgcgtgaact gttatttttg
     1381 aaattaatta attatgcggc caatacggaa ctgctgaagc ttgttcctca tacacgtttt
     1441 ttggatcttg cgatcgttcc gtattgcttt gtcaaaaata ttgcggtcgg ggaggcaagt
     1501 atccttgttt atcattctca tatggaaatg tggaagatcg aagagaggga actgatgctg
     1561 gaggcaaaaa agaatacccg tgaaattatg ggcagtgaaa ttatggcgct taaggaactg
     1621 gtagaggagg caggatgctg tgaagagctg cctgcaggca aagaaggaga ggaaacggaa
     1681 atgtacgtcc tttccaaccg gtataagcta aacggtgcca tatgtatgac ttatccggat
     1741 gtgctggacg cattttctga aaaaacaggt aaaaatctgt atgtactgcc aagcagtatc
     1801 cacgaagtga ttttgatccc ggcatcagga atgtatgaac tcgacagact gtcagaaatg
     1861 gtccatgagg tgaatatgac acaggtggaa caggaagaac tgctgtcgga tcatgcatat
     1921 tattatgaaa aaaacatcgg atttgacaag tcttttatga atgcctgcgc acgtaactaa
     1981 tttgtaatat tttcatttga tattttcttt tccctatgtt atgatatgag atatgtgaaa
     2041 ttgttgtcgg tcaatcaggc tgacagggca tgcactcgta gctcagggga tagagcaacg
     2101 gtttccggta ccgtgtgtcg ggggttcgaa tcctctcggg tgcagtacca tttcacatat
     2161 aaccaaggaa cgaatatttt ggttgtaagg aatgaaagga agtaaggtgt gaatatggca
     2221 ggaaataaag taaaagagtt tattgtaaaa cactatcggt actttattgt aggaggcctg
     2281 tttgttgttc ttgttgtagt attgattata ctgatggtaa aaaacaaaga caatacgtca
     2341 gaatctgatt ccgacgttga tgccgtactt gcggagagta atattgatgt gccaaaggat
     2401 aagtatgaag tcaatgcata tccggaggtc aatacgctgc ttgagtccta ttataatgcg
     2461 atggctgccg gggatgcgga tacaatggca tctttgagca atacgatgac ggatgaggaa
     2521 aagatccgta ttcaggtaac agccgagtat tatgaaggat tttctaatta tcaggtctat
     2581 acaaaaaaag gtccggaaga aaattcttat attgcatttg tgacttatga tattatgttt
     2641 acaggggcga cggtttccgc acctgcgctg gcatctgtgt acgtatgcac gaatgattcc
     2701 ggtgctcttt atattaataa agacaatctg acagacgaag agaatgctta ttttagagcg
     2761 atatctgtac aggatgatat tgaggagctg tgtgacgaag taaaggtggc atataataaa
     2821 gttctggagt ccgatgaaac gcttgcagca ttgatgccga ctttaaaaga tacgatcaac
     2881 cagcaggtaa aagaccagct ggcggctcag cagcaggcag aggctgaagc tgccgcacag
     2941 gctgaagctg ccgcacaggc agcagaacag gaagcttcgg ctacaatggt tcgtgcaacg
     3001 gacgttgtaa atatccgttc atcggcgagc acggaaagcg acgtacttgg aaaggctgcc
     3061 gtaggggatg aatatacccg ctatgaagaa atggataacg gctggagcaa gatcgattac
     3121 aatgggcagg aggcttatat caaatcagat tatctggaag tc
//
LOCUS       DAOSYE010000171         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22389_length_3160_cov_3.210950, whole genome shotgun sequence.
ACCESSION   DAOSYE010000171 DAOSYE010000000
VERSION     DAOSYE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22389_length_3160_cov_3.210950"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /gene="ptsP"
                     /locus_tag="PLU43_03985"
     CDS             complement(<1..1470)
                     /gene="ptsP"
                     /locus_tag="PLU43_03985"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOO27600.1"
                     /translation="MTKGMPEIVKKYIGKAVNCGIAIGNIRLYKKRTEMISKEFAGIA
                     QEKKRYEEAVAEARSQLEEVYKKAVGEVGEESAAIFTVQAMMADDQDFAAFVYQKIEM
                     EKVNAECAVMAAGDHFSAVFAAMEDVYMRERASDIRDISERLSAILAGGQKSAFSIKE
                     PSIIFADDLTPSETIQMDKKNILAFVTARGSANSHTAILARMMNIPAVVGLLAEIDAV
                     YENKTAIVNGSTGEVILEPNEEMLMAAQKSRTEEAQKEKQFLIYRGRKSMTTGGKKIN
                     VFANIGNVSDIALALENDAEGIGLFRSEFLYLGKNSYPTEEEQFQIYRQAAVSMAGKK
                     VIIRTLDIGADKQVAYFQLDKEENPALGYRAIRICLDRTAVFKTQLRAIYRAAAYGTI
                     AIMYPMITSVWEVKKIKSMIEEIRRELAEEGIAVGEVEQGIMVETPAAVMESDQLAKE
                     VDFFSIGTNDLTQYTLAVDRQNIRLDAFYDPHHPAVMKMI"
     gene            complement(1491..1748)
                     /locus_tag="PLU43_03990"
     CDS             complement(1491..1748)
                     /locus_tag="PLU43_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO27601.1"
                     /translation="MKRFIFVVKEKIGIHARPASQLVRKAAAYKSAITIEKDGKSADV
                     KRLIALMALGVQCGEKVTFMIEGEDEEIAAVQLEEYCNHNL"
     gene            complement(1753..2868)
                     /gene="nagA"
                     /locus_tag="PLU43_03995"
     CDS             complement(1753..2868)
                     /gene="nagA"
                     /locus_tag="PLU43_03995"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOO27602.1"
                     /translation="MQIRNVKIYHEDKGFVTGDISMRNGVIAACAAGEPFIDGTGLYA
                     IPGLTDIHFHGCVGYDFCDGTGEAIAHMAEYQEKNGITTICPATMTLGEPDLSNICSA
                     AAAYKNNKGAILCGLNMEGPFLSLQKKGAQNGAWIRKPDAELFRRLQTKAGGLCRLVS
                     LAPEEEGALSFISELKDDVIISLAHTTADYHTAMEAFRLGARHVTHLYNAMPPFAHRE
                     PGVVGAALDTQNCNVELICDGVHVHPSVVRATFRMFGEDRIILISDSMRAAGMPDGQY
                     TLGGQDVMVRGNAATLCADGALAGSVTNLMDCMKNAVQQMGIPLETAVKCAAVNPAKA
                     IGIYDQYGSIEPGKAANIVLLNEQLEIEAVFLRGKRR"
     gene            complement(2890..>3160)
                     /locus_tag="PLU43_04000"
     CDS             complement(2890..>3160)
                     /locus_tag="PLU43_04000"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOO27603.1"
                     /translation="LTKSTIEANARLFKNEGEVPRYAYTMGIRTIMQAKKILVIVSGR
                     NKADIVKKSFFGPVAADVPASILQMHNDVTLVGDADALSLCEMPK"
BASE COUNT          792 a          881 c          520 g          967 t
ORIGIN      
        1 aatcattttc ataacagcag gatgatgcgg atcataaaaa gcatccagtc ttatattctg
       61 acggtcaact gccaatgtat attgtgtcaa atcgttcgtt ccgatactga aaaaatcgac
      121 ttcctttgcc aattgatcac tttccataac agctgcaggc gtttctacca tgattccctg
      181 ttcgacctct ccaacagcga tcccttcttc tgctaattcc cgccgtattt cttcaatcat
      241 agatttaatc ttttttactt cccaaacaga agtgatcatc ggatacatga ttgcaatcgt
      301 tccatatgcg gctgctctgt agattgctcg cagctgtgtc ttaaaaacag cagtcctgtc
      361 aagacaaatt cggattgcac gataaccaag cgccggattt tcttctttat ccagctgaaa
      421 atatgcaacc tgtttatccg ccccaatatc aagggtcctg ataatcactt tctttcctgc
      481 catggatacg gcagcctgcc tgtagatctg aaactgctct tcttccgtcg gatatgaatt
      541 tttgcctaaa tacaaaaatt cacttcgaaa aagaccgatc ccttctgcat cgttctctaa
      601 agccagcgca atatccgata cattgcctat attggcaaat acatttattt ttttcccgcc
      661 tgttgtcata ctttttctgc cacgatagat gagaaactgc ttttccttct gcgcttcttc
      721 cgttctgctc ttctgagcag ccatcagcat ttcttcattt ggctcaagaa tgacttctcc
      781 tgtagaacca ttcacgattg ccgtcttatt ttcatatacg gcgtcaattt ctgcaagaag
      841 ccctacaacc gccggaatgt tcatcatccg cgcaagaatc gccgtatgtg aattcgcaga
      901 accacgtgca gttacaaatg caaggatatt tttcttgtcc atttgaatgg tttcactcgg
      961 cgtcagatca tctgcaaata taatagacgg ttcctttatg gaaaatgcac ttttttgacc
     1021 gccggctaat atggctgaca ggcgttctga aatatctctg atgtcacttg cccgctcccg
     1081 catatataca tcttccatag cagcaaacac tgctgaaaaa tgatcgcctg ctgccataac
     1141 agcacattcc gcatttacct tttccatctc aatcttctga tatacaaaag cagcaaaatc
     1201 ctgatcatcc gccatcattg cctgaacggt aaatattgca gcgctttctt cccccacctc
     1261 tcccactgct tttttatata cttcttcaag ctggctcctg gcttctgcga ccgcctcttc
     1321 ataacgcttc ttttcctgtg caattcctgc aaattctttg ctgatcattt ctgttctttt
     1381 tttataaagc cgtatgtttc ctattgcaat tccgcaatta acagcttttc caatatattt
     1441 tttcacgatt tccggcatcc ctttcgtcat acacaaaacc tgcagcattc ctacagatta
     1501 tgattacaat attcttccag ctgcacggct gcaatttcct catcttcccc ttcaatcata
     1561 aaagtgacct tttcaccgca ctgtacacct aatgccatca aggcaatcaa ccgcttcaca
     1621 tccgcacttt ttccatcttt ctcgatcgtt atggcacttt tataagctgc tgcttttcgc
     1681 acgagctggc ttgcaggtct cgcatgaatt ccaattttct cttttacaac aaatataaaa
     1741 cgcttcataa gcttatctcc tttttcctct taaaaatact gcttctattt caagctgttc
     1801 attcagcagc acaatattag ctgcctttcc cggctcaata cttccatact ggtcgtaaat
     1861 acctatggcc tttgcgggat ttaccgccgc acatttcact gctgtttcca atggtattcc
     1921 catctgctgc accgcatttt tcatacagtc catcaaattt gtgactgatc cggccaatgc
     1981 gccgtccgca cacaacgttg ccgcatttcc tctgaccata acatcctgtc cgcctaatgt
     2041 atactgcccg tccggcatac cggccgctct catactgtca ctgattaaaa tgatgcggtc
     2101 ctccccaaac atacgaaacg tcgctcttac tacacttgga tgtacatgaa caccatcaca
     2161 tatcagttcc acattgcagt tttgtgtatc aagggccgct ccaacaacac ccggctctct
     2221 atgtgcaaac ggcggcatcg cattatacag atgcgtaaca tggcgggccc ccagccgaaa
     2281 ggcttccata gccgtatgat aatcggctgt tgtatgcgca agtgaaataa ttacgtcatc
     2341 cttcagctcg gaaataaaag aaagcgcacc ctcttcttcc ggcgcaagcg ataccagtct
     2401 gcataatccg ccggcttttg tctgcagtct tctaaacagc tctgcatccg gtttgcgaat
     2461 ccacgcacca ttctgtgcac cctttttctg caaagacaaa aaaggacctt ccatgttcag
     2521 accgcatagt atggctcctt tattattttt atatgcggcc gctgcgctgc aaatatttga
     2581 aagatccggc tcacccagtg tcattgtcgc cgggcagatc gttgtaatac catttttttc
     2641 ctgatactct gccatatgcg cgatcgcctc acctgtaccg tcgcaaaaat catagccgac
     2701 acatccgtga aaatgaatat ccgtaaggcc cggaatcgca taaagaccgg ttccatctat
     2761 gaatggctct cccgctgcac aggcagcaat cacaccattc ctcatagaaa tatcccctgt
     2821 tacaaatcct ttgtcttcgt gatagatctt cacattcctt atctgcattg cagacacctc
     2881 ccaaataaat tattttggca tctcacacaa cgacagcgca tccgcatctc caaccagcgt
     2941 cacatcatta tgcatttgca gaatggaagc cggtacatct gccgcaaccg gaccaaaaaa
     3001 tgattttttt acaatatccg ctttattcct gccgctcaca ataacaagaa tcttttttgc
     3061 ctgcataatc gttctgatcc ccatcgtata ggcataacgc ggaacctcac cttcgttttt
     3121 gaacaatctg gcatttgctt ctatcgtact ttttgtaagc
//
LOCUS       DAOSYE010000172         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22416_length_3158_cov_3.160168, whole genome shotgun sequence.
ACCESSION   DAOSYE010000172 DAOSYE010000000
VERSION     DAOSYE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22416_length_3158_cov_3.160168"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /gene="uvrA"
                     /locus_tag="PLU43_04005"
     CDS             complement(<1..1725)
                     /gene="uvrA"
                     /locus_tag="PLU43_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO27604.1"
                     /translation="MENRDEKKFIRIRGANEHNLKNVTIDIPRNEFVVLTGLSGSGKS
                     SLAFDTIYAEGQRRYMESLSSYARQFLGQMEKPEVESIEGLPPAISIDQKSTNRNPRS
                     TVGTVTEIYDYFRLLYARIGIPHCPECGKEIKKQTVDQMVDALMKLPEGTRIQLLSPV
                     VRGRKGEHVKVLERARKNGYVRVRIDGNLYELSEEIRLDKNLKHNIEIIVDRLVIKPD
                     IEKRLTDSLENVLELSDGLVFADVVGGEQLTFSQSFACPDCGISIEEIEPRSFSFNNP
                     FGACPVCAGLGYKMEFDEDLIIPDKSLSLAEGAVAVPGWQSCTDPSSFTYAILKALTK
                     EYRFDLNTPYEKLPADIQDMLVNGTNGREVKVHYKGMRGEGVYDVAFEGLIKNAERRY
                     RETGSDIMRQEYESFMRITPCKECGGMRLKKGALAVTVGEKNIYELTSMSVRDLFGFM
                     EKLELTKQQELIGHQILKEIKARLGFLLEVGLDYLTLTRATGSLSGGEAQRIRLATQI
                     GSGLVGVVYILDEPSIGLHQRDNDRLLNALKNLRDLGNSLIVVEHDEDTMLAADYLID
                     IGPGAGEHG"
     gene            complement(1715..>3158)
                     /locus_tag="PLU43_04010"
     CDS             complement(1715..>3158)
                     /locus_tag="PLU43_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27605.1"
                     /translation="ALCMNTKKMQISEIDNKNLTEWPGITLTLSTRTELEDYVDDRIG
                     FREQAINSYIELNDKLFHVMVHPLFMYGKDGHIFYKDASYIAAYQRLNTDPDYLDSFV
                     DFLQKTKTYLESKDIDFLYFLCPDKKTIYSEYFPDSIHVNTANESVTAYMDEKLKETD
                     IEYIIPVEQLLEAKKTQVVYNKLYDATHWNEFGSFLGHKLIDAHIQKWFTDVPKLTED
                     DYDLTYVTMETLDIAKFPIHDEVPVYTLKTDNSQDATEYLMPYLQCTTTTFYSHYIYP
                     DCPNDDVLLIFTDSYFGNYQRYYNNRFKEVYFVHRQNYDYLQYFVNLVFPDAVIFETA
                     ERSITSEMPIMADFSQYYYEEPFDGQGDVLKSDDLSYALTGVSGIRRDGSTLYLDPDE
                     GDNIIRMNGMFRAKDRDAAYDIYVKIGDTYLEADYCALHRNAETDGIQEFSVSVQRRY
                     MSEGPIELIAVDRNTKQEYCLETFEVVYGK"
BASE COUNT          806 a          832 c          625 g          895 t
ORIGIN      
        1 gccatgttct ccagcgccgg gaccgatatc gatcagataa tctgccgcaa gcatcgtatc
       61 ctcatcatgt tcaacaacga tcagggagtt cccaagatcg cgcagattct tcagcgcgtt
      121 caaaagcctg tcgttatccc gctgatgcag gccgatgctc ggttcatcaa gaatataaac
      181 gacgccgaca agtcctgaac cgatctgtgt ggccaggcgg atccgctgtg cttctccgcc
      241 ggacaatgaa cccgttgcgc gcgtaagcgt caggtaatcc aggccgactt caagcaaaaa
      301 tccaagccgg gctttgattt ctttcaatat ctgatgaccg atcagctcct gctgttttgt
      361 cagttcaagc ttttccatga atccgaacag gtctctgacc gacatggacg tcaattcata
      421 aatatttttt tccccgaccg taacggccag tgctcccttt ttcagccgca taccgccgca
      481 ttccttacat ggcgtgatgc gcatgaacga ttcatattcc tgcctcataa tatcggaacc
      541 ggtttctctg taacggcgct ccgcattttt gatcagccct tcaaaagcaa cgtcataaac
      601 gccttcgccc cgcatgcctt tatagtgtac tttgacttcc ctgccattcg taccgttgac
      661 aagcatatcc tgtatatcgg ccggcagctt ttcgtagggc gtattcaaat caaaccggta
      721 ctcttttgtc agtgctttta aaattgcata ggtaaagctg gacggatccg tacaggactg
      781 ccagcccgga acagcaaccg ctccttcggc aaggctcaag gatttatccg gaatgatcag
      841 atcttcatca aactccatct tatagccaag acctgcacag acaggacaag ctccgaacgg
      901 gttgttgaag gaaaagcttc gcggttcgat ctcctcaatg ctgatcccgc agtccggaca
      961 ggcaaagctc tgggaaaacg taagctgctc cccgccgacc acgtccgcaa atacaagtcc
     1021 gtctgaaagc tccagcacat tttccagaga atcggtcaga cgtttttcaa tatcgggttt
     1081 gatcacaagg cggtcaacaa tgatctcaat attatgcttt aaatttttat caagtctgat
     1141 ttcttcagaa agctcatata aatttccgtc tatccggacc ctgacatatc cattttttct
     1201 tgcacgctca agcactttga catgctcgcc cttgcgcccc ctgacaaccg gcgacagcag
     1261 ctgtatcctt gtgccttccg gcagtttcat aagcgcatcc accatctggt ctaccgtttg
     1321 ttttttgatt tccttcccgc actccgggca atgcggaatt ccgatacgcg cataaagcag
     1381 gcggaaataa tcatagatct ccgttacggt tccgactgtg gaacgcggat tcctgttcgt
     1441 cgatttctga tcgatcgaga tcgccggcgg aaggccctca atgctttcca cttccggctt
     1501 ctccatctgt cctaaaaact gcctcgcgta agaggacaaa gactccatat agcgtctttg
     1561 cccttctgcg taaatcgtat caaatgcaag tgaggatttc ccagacccgc ttaggcctgt
     1621 caaaaccaca aattcattgc ggggaatatc gatcgtaaca tttttcaggt tatgctcatt
     1681 ggcgcccctg atccttataa attttttttc atctctattt tccatacact acctcaaatg
     1741 tctccaggca atattcctgt ttcgtattac ggtcaacggc aatcagctcg atcgggcctt
     1801 ccgacatata acgtctctgc acactgacgg aaaactcctg aataccatcg gtttccgcat
     1861 ttctatgcag cgcacaataa tccgcctcca aataggtgtc tccaattttt acataaatat
     1921 cataggcggc atccctgtct tttgcccgga acatgccgtt cattctgata atattgtctc
     1981 cttcatccgg atccagatac agtgtggaac cgtctcttct gattccgctg actcccgtca
     2041 gtgcatagct taaatcatcc gacttaagaa catctccctg gccgtcgaac ggttcttcat
     2101 aataatactg actgaaatcg gccatgatcg gcatttcact tgtaatgctc cgctccgctg
     2161 tctcaaagat caccgcatcc ggaaatacaa gatttacaaa atactgcaga taatcatagt
     2221 tctgcctgtg tacaaaataa acttccttaa accggttatt ataatatctt tgataattcc
     2281 caaaatagct gtccgtaaag atcagaagca catcgtcatt tggacagtcc ggataaatat
     2341 aatgggaata aaacgtcgtc gtcgtacatt gcagatacgg catcaggtat tccgttgcat
     2401 cctggctgtt gtcggttttc agtgtatata ccgggacctc atcatggatc ggaaattttg
     2461 cgatatctaa cgtctccatc gttacatacg tcagatcata atcatcttcc gtaagcttcg
     2521 gcacatccgt aaaccatttc tggatatggg catcgatcag tttatgaccg agaaaagaac
     2581 cgaattcatt ccaatgggtc gcatcataaa gcttgttata aacgacctgg gtctttttgg
     2641 cttccaaaag ctgttcgacc ggaataatat attcgatatc cgtttctttc agcttctcat
     2701 ccatatatgc ggtaacactt tcgttcgccg tattgacatg aatgctgtcc ggaaaatact
     2761 cggaatagat cgtcttttta tcaggacata aaaaatacaa aaaatcaata tccttgcttt
     2821 ccagatatgt tttcgttttc tgcagaaaat ccacaaagga atccagataa tcaggatctg
     2881 tgttcagtct ctgataggcc gcaatatagg aagcatcttt ataaaaaata tgtccgtcct
     2941 tgccgtacat aaagagcgga tgcaccatga catgaaacag cttgtcgttc agttcgatgt
     3001 aggaattgat cgcctgttcc ctgaacccga tacggtcatc cacataatct tcgagctccg
     3061 tgcgtgtgct gagcgtcagc gttatgcccg gccattccgt cagattctta ttatctattt
     3121 ctgaaatctg cattttcttt gtattcatgc aaagtgca
//
LOCUS       DAOSYE010000173         3156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22440_length_3156_cov_2.960013, whole genome shotgun sequence.
ACCESSION   DAOSYE010000173 DAOSYE010000000
VERSION     DAOSYE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22440_length_3156_cov_2.960013"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2460)
                     /locus_tag="PLU43_04015"
     CDS             complement(<1..2460)
                     /locus_tag="PLU43_04015"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOO27606.1"
                     /translation="MEKGRVKIKEAYTKKRKLLFDGLTVLLLAAIAALIFYQYFGVSY
                     EDVRIRGNSIDCFGEGFVSEETGAGLSDFPVCLKRGESITITKQLPDEIGQDEVLSFL
                     NNGFSVEAFVDEEPVYQYGLANAAEFGKETGQLWNYISLQETMAGRQLTIRLYAYADR
                     AYLGSSSFYIGSTGAMDMYILKMNFCVLFGTVLIVLVSVLFLIYSFYLKKKSLPGEKL
                     FLYAGTFSLLFAVWIFADSTLVQFFTGKMTARYLVSCYAFMLMFLPLLLLFREGCVAH
                     KIWLDLLQMLFGTAFVACTVLYAAKVMPLSESLFLFYFLVAAAFISAVIITVNEYRTY
                     HNKAIIEPFVVLAILIVAGTGEIAARYAGTVTDSSPVFRCGLLLFVLFFFITILRRSF
                     MILRKNMMAEYYKKLAFVDAVTKGNTQQYFEEMVPKQLENGINYGVVYFDIISFKMVN
                     DDVGRTEGNHVLREMYQSLSNGIGAHEILAHAGGAHFIMLLENSRVWDISMRLYNMKK
                     AINQLPVGQMKKNQISVCAGIYLAGHEKLSVSQMIDRAIMARNEDDIEIINGFGCKIY
                     SDEIRLRMVRENALKNRMLQGMEKGEFKVYLQPKVLLMTGRVSAAEALARWEDPKEGM
                     IFPAEFIPIFENNGAIIRMNLYMLRGVLDILKDWLKRGITPVVISVNLSKISIHNPIF
                     MSEFSKIMEDPATPARYLEFEFTENAAYENPAKLNALIEQIHEWGATCSIDDFGCSYS
                     NLNMLKDVPVDVLKLDKEFFGYEQKNKAKAFKIIESIIRLTHDIGMEIVAEGVEDESQ
                     VAFLKEIDCDMIQCFYHSKPLS"
     gene            complement(2463..>3156)
                     /locus_tag="PLU43_04020"
     CDS             complement(2463..>3156)
                     /locus_tag="PLU43_04020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO27607.1"
                     /translation="NIAIMMLALLLTAAFILRFASNIVMVIKKMMEFLKEMSLGNLST
                     QLSPVVVNREDELGEMGNFTVKVQASLRKLIERDVLTGLYNRRSGENMLDKVKAGGMT
                     YAVAIGDIDFFKKFNDNFGHDCGDVVLKEVAAVLDNGMRGIGFVARWGGEEFLLVFDN
                     MNGLAAGVEADRILQMVRENTVRYNGQEHTVTMTFGVAEGNAEKPINIQIKEADEKLY
                     RGKESGRNRVIV"
BASE COUNT          883 a          861 c          567 g          845 t
ORIGIN      
        1 ggaaagtggt tttgaatggt aaaagcactg gatcatatca caatcgattt ccttcaaaaa
       61 tgcaacctgt gattcgtctt ccaccccctc ggcaacgatc tccataccga tatcatgggt
      121 cagcctgata atagattcaa taatcttaaa agcttttgct ttatttttct gctcgtaccc
      181 gaaaaactcc ttatccagct tcagcacatc taccggcaca tccttgagca tattcagatt
      241 ggaataactg catccgaaat catcaatgga acaggtcgca ccccattcat ggatctgctc
      301 gatcagggca ttcagcttgg caggattttc atatgcagca ttttccgtga attcaaattc
      361 caaataccgg gccggcgttg ccggatcttc catgatcttt gaaaattcag acatgaagat
      421 cggattatgg atactgattt tggacagatt cacggaaatg acgaccggtg tgatccctct
      481 tttcagccag tcctttaaaa tatccagaac ccctcttaac atatagagat tcatcctgat
      541 gatcgcgccg ttattttcaa atatcggaat aaattctgcc ggaaagatca tgccttcctt
      601 tggatcctcc caccgggcaa gtgcttccgc cgcgctgacc cgaccggtca taagcagtac
      661 cttcggctgc agatacactt taaactctcc tttttccatt ccctgcagca tgcggttttt
      721 aagtgcgttc tccctcacca ttctaagccg gatctcatct gagtagatct tacagccaaa
      781 tccgtttatt atctcgatat cgtcctcatt tctggccatg atcgcgcggt cgatcatctg
      841 tgagacagac agcttctcat gccctgccag ataaattccg gcgcataccg atatctgatt
      901 ttttttcatc tgcccgaccg gcagctgatt gattgctttt ttcatgttat aaagccgcat
      961 ggaaatatcc cagacacggc tgttctccaa aagcataata aaatgcgcgc cgcctgcatg
     1021 ggcaagtatt tcatgcgcac cgattccgtt tgaaaggctt tgatacattt cacgcaaaac
     1081 atggttgccc tctgttctgc ccacatcatc gtttaccatt ttaaaactga tgatatcaaa
     1141 atagacaact ccataattta tgccgttctc cagctgtttt ggcaccattt cctcaaaata
     1201 ctgctgtgta tttcctttcg taacagcatc cacaaatgcc agttttttat aatattccgc
     1261 catcatgttt ttcctaagga tcatgaagct tctgcgcagg atcgttatga aaaaaaacag
     1321 tacaaacaat aaaagaccgc agcggaatac tggcgaactg tccgtgactg ttcccgcata
     1381 acgggctgct atttctcccg ttcccgcaac gatcagaatt gccagcacga caaatggttc
     1441 aatgatcgcc ttgttatgat acgtcctgta ctcattcaca gtaatgatca cggcactgat
     1501 aaaggctgct gctactaaaa aataaaaaag aaaaaggctc tctgacagcg gcatcacttt
     1561 tgccgcatat aataccgtac atgcaacaaa tgccgttcca aaaagcatct gcagcagatc
     1621 aagccagatc ttatgcgcaa cacagccttc cctgaacaaa agaagcagcg gaagaaacat
     1681 cagcataaat gcataacagg acaccagata tctggctgtc atctttcctg taaaaaactg
     1741 caccagcgtg gagtcagcaa agatccagac cgcaaaaagc agggaaaagg tccctgcata
     1801 aagaaacagt ttttcaccgg gcaggctttt cttttttaaa taaaaagaat agatcagaaa
     1861 aagaacgctg accagaacga tcagtaccgt accaaacaga acacaaaaat tcattttcag
     1921 aatatacata tccatggcac cggtacttcc gatataaaaa gaagaactcc caagataggc
     1981 cctgtccgca taggcataca gccggatcgt cagctgccgg cccgccatgg tttcctgcag
     2041 actgatataa ttccagagct gtcccgtttc ctttccaaat tccgccgcat tggcaagccc
     2101 atactgataa accggttcct catcaacaaa tgcctcaacg ctgaagccat tgttcaaaaa
     2161 agaaagcacc tcgtcctgcc cgatctcatc cggcaactgt ttcgttatcg taatactctc
     2221 gccccgcttc agacagacag gaaaatcaga aagccctgct ccggtctctt cactgacaaa
     2281 cccctctcca aagcaatcaa tcgaatttcc acgaatacgc acatcctcat aagagactcc
     2341 aaaatactga taaaaaatca acgctgcgat cgcggcaagc agtaaaaccg taagaccatc
     2401 aaacaacagc tttctcttct ttgtatatgc ttctttgatc ttgacccgtc ccttttccat
     2461 agctatacga ttacgcgatt cctgccgctt tcctttccac ggtacaattt ttcgtccgct
     2521 tctttgatct gtatattgat aggcttttcg gcatttcctt ccgccacgcc aaatgtcatc
     2581 gtgaccgtat gctcctggcc attatatctt accgtatttt cgcgaaccat ctgcaggatc
     2641 ctgtcggcct ccacacctgc cgcaagtccg ttcatattat caaacacgag cagaaattcc
     2701 tcgccgcccc agcgcgcaac aaatccgatc ccgcgcatac cgttgtccag caccgcagcg
     2761 acctccttta agacaacgtc cccgcagtca tggccgaaat tatcattgaa ctttttaaaa
     2821 aaatcaatat ccccgatagc aactgcatac gtcatgccgc ccgccttcac tttatccagc
     2881 atattttcgc cgcttcttct gttataaagg ccggtcagta catctctttc tatcagcttt
     2941 cgaagcgacg cctgcacctt tacggtaaag ttgcccatct cgcccagttc gtcttccctg
     3001 tttacaacga ccggggaaag ctgtgtggac agatttccaa gtgacatttc cttaagaaat
     3061 tccatcatct ttttgatcac cattacaata ttggaagcaa accgtaaaat aaaagctgcc
     3121 gtaagcagta aagccagcat catgatcgct atattc
//
LOCUS       DAOSYE010000174         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22497_length_3151_cov_5.223837, whole genome shotgun sequence.
ACCESSION   DAOSYE010000174 DAOSYE010000000
VERSION     DAOSYE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22497_length_3151_cov_5.223837"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..721
                     /locus_tag="PLU43_04025"
                     /pseudo
     CDS             146..721
                     /locus_tag="PLU43_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            838..1971
                     /locus_tag="PLU43_04030"
     CDS             838..1971
                     /locus_tag="PLU43_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOO27608.1"
                     /translation="MKRILRIGMDVHSTNYTLCAMEPVIGENDRIFVNIQVTPDYKNI
                     LMFIENLKQKLGVDNEYDIQCGYEAGCLGYSLYNQLTAVGVKCVILAPTTMLTQQGQR
                     VKTDTRDALMIAQCLAYGGYHAVYIPAEEDDSVKEYLRMRDDHKLALKKIKQQINAFC
                     LRHGYHFPNTKWTIAHWKWLKNLELKDMYRESLNEYMASYEEQEAKIERFDQRIEELA
                     SQEAYQEKVKKLECFLGIKTHTALSLIVETGDFERFAKGNTYAAFLGLAPGECSSGEK
                     INRTGITKAGNSHLRLLLIESAGGICKGAIGHKSKSLRARQNGNTAEVIAYADKANAR
                     LRSRYYKFIRHGKARNVAVAAVARELACFVWGMMTDNISLKAA"
     gene            complement(2460..2933)
                     /locus_tag="PLU43_04035"
     CDS             complement(2460..2933)
                     /locus_tag="PLU43_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO27609.1"
                     /translation="MERIIGIGEIAVSNRHEDIIKTFSLASCVAVAVYCSVNGTAGMI
                     HVALPEKGRLCCEFENQPCYYAEQGVPLLIDKMCLEYNCPASGLEIELFGGAESIRIN
                     DVFQIGRKNISVIKELLEDRNLKYKANEIGGRNSRTLIMNVSTGEKIVICRPLII"
     gene            complement(2933..>3151)
                     /locus_tag="PLU43_04040"
     CDS             complement(2933..>3151)
                     /locus_tag="PLU43_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOO27610.1"
                     /translation="ANAMGVILTGMGYDGARGILAMRRKGAVTIGQDEASCVVYGMPK
                     VAFDIGGVVRQAALENIPGMMMRCLGDK"
BASE COUNT          996 a          653 c          673 g          829 t
ORIGIN      
        1 tgttttgtta tgtctgcacg gacactttgg ctgtgcgcga tattatgcgc agctgatgga
       61 acagctgaag gactggcatg tttacagtgt tgatcagcgt ggtcatggct gaagtgatca
      121 tgctgcggcc ggacattatg aaagaagcga ttatatcggc gacattctga ccttcatgca
      181 tactgtttta aatgatcagg ccatatttat tttaggccac tcacttggcg gaataaacgc
      241 atatcaggtg gcagcaaggc gcactgatct ggtaaatggt cttttaattg aagatgccgg
      301 agctattgag aaggacgatg cttccttttc atccaaaatc attgactatg ctcccacact
      361 gaaagaactc ggggacagtc tgaagaaatt taaaatttat gatgcagcgt acttcttaga
      421 aagtgcggaa gaaaccgaaa aaggctggca ttaccggttc gacaaagcca acctggccga
      481 atcacaaaaa cggctgaatg gtaactggtg gaatgatttt ttggcatcct cctgccccgc
      541 cctgctctta catggtaaaa aatcatgggt tgtcaaacag gcgcacattg aagagatggc
      601 agcaaaaaga aaaaacacgg aatatgccgt atttaacaaa agcagtcata cggttaatct
      661 tgacgagccg gcactgtttt ttgaaaccgt cagacaattt ctttacaaga acagcatata
      721 acaatttaga taatatgaag tgaccccaca tttgtagcaa cgtggattta cctgtatact
      781 agagtttgat aagaacaatg gtaacaggga ttaccgcata caaaaggagg tcaccttatg
      841 aaaagaatac tacgaattgg aatggatgtc cacagtacaa attacacttt atgtgcaatg
      901 gaaccggtga ttggagaaaa cgacagaatc ttcgtaaaca ttcaggttac accagattat
      961 aaaaatatac tgatgtttat tgaaaattta aagcagaaac tcggagttga caacgaatat
     1021 gatattcaat gtggttatga ggctggatgc cttggatatt ccttgtataa ccagcttaca
     1081 gcagtaggtg taaaatgtgt gattcttgct ccaacaacaa tgcttacaca gcagggacaa
     1141 agagtaaaga cagacacaag agatgccttg atgattgcgc agtgtttagc ttacggtgga
     1201 tatcatgcag tttatattcc tgccgaagaa gatgattctg taaaagaata tcttcgaatg
     1261 agggatgatc ataagcttgc attgaaaaag ataaaacagc agattaatgc gttctgcctg
     1321 agacatgggt accattttcc taacacaaag tggacaattg cccactggaa atggttaaag
     1381 aatctggaac tgaaagacat gtatcgagag tcattaaatg aatatatggc ttcatacgaa
     1441 gaacaggaag caaagattga acgatttgat cagcgaattg aagaacttgc ttcacaagaa
     1501 gcatatcagg agaaagtgaa aaaactggaa tgctttctgg gaataaaaac acatacagca
     1561 ttgtcattaa ttgtggagac aggggatttt gaaagattcg caaagggaaa tacttatgct
     1621 gcatttcttg gacttgcgcc gggagaatgt tccagcggag aaaagataaa ccgaacagga
     1681 ataacaaaag caggaaacag tcatttaaga ttattattga tagaatcagc gggaggcatc
     1741 tgtaaaggag caattggaca taagtccaag tcactgcgtg caaggcaaaa tggtaatacg
     1801 gcggaggtca tagcatatgc ggataaggca aatgcaagac ttcgaagccg atactataaa
     1861 tttatacgtc atggaaaagc aagaaatgta gcagtggctg cagtagcacg agaattggct
     1921 tgttttgtat gggggatgat gacagacaat atcagtctga aagcagcata ggtacatcat
     1981 tgtcatctgc cagtcaagtg tgaaatacaa gaagagcttg tatttcgccg ctaaagcggc
     2041 tacgcacctg acagccatct ggccatgatg taaagggtaa tcaagcagaa ataagcagta
     2101 aactgcagta tttctgccat gtaacaaatt aaatatctga cagtatcttg aaggagcttc
     2161 gatcacttca aggttcgagc taactgcgta cctcctatgt gtgcacgtaa aaccgtgatc
     2221 cacgatttta gatcgcagag ctctcggcga accattagcc tgtaggtaac caatccacga
     2281 ataacagaat ggccaactgc cgggaactgt taaatcaggc tctttccaga tgctgtcaga
     2341 aaacaaatgt gatctaatgt tgagagaaaa agaaaaattc tttgatttac ctcttgacaa
     2401 aggtcacttc ataacagggg ggagaatctc tctatccggt tttattttaa accgccctct
     2461 taaataataa gcggccgaca gatcacgatc ttttctcctg tcgaaacatt cataatcagt
     2521 gtcctgctgt ttctccctcc tatttcattc gctttatact ttaaattacg gtcttccagt
     2581 aattctttga taacggaaat atttttcctg ccgatctgga aaacgtcatt tatgcgtatt
     2641 gattccgctc cgccaaataa ttctatctcc agtccggaag ccggacaatt gtattccagg
     2701 cacattttat cgatcagcag aggaaccccc tgttccgcat aataacacgg ctgattttca
     2761 aattcacagc atagtctgcc cttctccggc agcgcaacat gtatcattcc tgcggtaccg
     2821 ttcacagaac aatacactgc aacagcaacg caggaagcca gtgaaaatgt ctttataata
     2881 tcttcatgcc tgtttgacac cgctatttca ccaattccaa ttatacgctc catcacttat
     2941 cacccaaaca tctcatcatc attccaggga tgttttcaag agctgcctgc cgtacgactc
     3001 cgccaatatc aaacgcaacc ttcggcattc cataaacaac gcagctcgct tcatcctgcc
     3061 cgatcgttac cgcgcctttt cttctcattg ccagtatccc ccttgcaccg tcatatccca
     3121 tgccggtaag aattactccc atcgcgtttg c
//
LOCUS       DAOSYE010000175         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22501_length_3151_cov_3.433140, whole genome shotgun sequence.
ACCESSION   DAOSYE010000175 DAOSYE010000000
VERSION     DAOSYE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22501_length_3151_cov_3.433140"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..391
                     /gene="crcB"
                     /locus_tag="PLU43_04045"
     CDS             26..391
                     /gene="crcB"
                     /locus_tag="PLU43_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOO27611.1"
                     /translation="MINCLFAGIGGFIGTVCRYLIGLIPLEHSSGFPFKTLMINITGA
                     FLIGLITAAAAKNKALSPQLVTMLKVGICGGFTTFSTFAYETTDLIHSGKPILALVYV
                     LTSTVLGTLAVFGALLLAK"
     gene            404..1375
                     /locus_tag="PLU43_04050"
     CDS             404..1375
                     /locus_tag="PLU43_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO27612.1"
                     /translation="MMNLGIAGAGLIVHHFLSFIHEVSGIRLQAIYARDNKEQRLTKL
                     CMENQINSFYTDYEKMLSDKQIDTVYVAVSNHMHYIFVKKALLSGKHVICEKPFTVTL
                     SEFDELCRIAREKKLFLLEAITTLYFPNYLAIKEAVKTIGDIRIITCNFSQYSSRYDD
                     FKKGIIKPCFDPAMAGGALMDLNVYNIHFVVNLFGEPKTVRYYPNIRQGVDVSGILIL
                     SYETFQCVLIGAKDCGAPPCTNIQGDKGCIYMESPANTCARFEVILNNQKPDKKELNK
                     DFHRMYYEFAAFERMLKEGDMETAATMLEQSRCVMKVLEQAKAGMII"
     gene            complement(1368..2069)
                     /locus_tag="PLU43_04055"
     CDS             complement(1368..2069)
                     /locus_tag="PLU43_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOO27613.1"
                     /translation="MPEKEIEKEIRTRLFELQDLKYKAFHSRLMPTIDPDTIIGVRTP
                     ALRNLVKEYKKRPEVSEFLKILPHRYYEENNFHGFLISEITDYDTCIKSLDAFLPYVD
                     NWATCDLMSPKPFKKHPPHLIEQIKSWINTKQTYTVRFAVEMLMSFYLDENFDDIYPA
                     LVAQIRSEEYYVNMIIAWYFATALAKQYDSILPYITEQRLEKWTHNKTIQKAVESYRI
                     TDEQKAYLRTLKISK"
     gene            complement(2089..2892)
                     /locus_tag="PLU43_04060"
     CDS             complement(2089..2892)
                     /locus_tag="PLU43_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOO27614.1"
                     /translation="MEQEQEKQILSNEKKEKTGRNHSGIIIAGIAGISIIACTVILTF
                     GFLNYKSKAGAYGITATGSASCDFESDLIVWRGSFSAFGETTSDAYRTIKEDTQTVRE
                     YLEENGITEDEMVFSSVNINRHYNSLYNEDGNYVGDELAGYDLYQDVCITSSDVDKVE
                     KISRDITGLIESGIEFTSDAPEYYYTKLDELKLQLIEDATANAKERIDIIAEGTGSKA
                     YALASASLGVFQITAQNSASEEYSYGGTFNTSSRDKTATITVKLNYNTK"
BASE COUNT          860 a          608 c          724 g          959 t
ORIGIN      
        1 ccctctattg taaaggagtt ttgtgatgat aaactgtctt tttgcaggta ttgggggttt
       61 tatcgggaca gtatgccgat atttgatcgg tctgattccg ctggaacaca gcagcgggtt
      121 tccgtttaaa acactgatga tcaatattac cggtgctttt ttgatcggtt tgatcacagc
      181 agcagcggca aaaaacaagg cactgagtcc acagcttgta acgatgctga aagttgggat
      241 ctgcggagga tttactacat tttcaacgtt tgcttatgaa acgacggatc tgatacacag
      301 cgggaagcca atacttgcgc tggtgtatgt tctgacaagc actgttttag gaacgctggc
      361 agtttttggt gcgttgttgc tggcaaaata gaagggacat caaatgatga atttgggaat
      421 agcgggcgcc ggactgatcg tgcatcattt tctttcattt atccatgaag tgagcgggat
      481 tcgcctgcag gcgatctatg caagggataa taaggagcag cggctgacaa agctttgtat
      541 ggaaaatcag ataaacagtt tttatacgga ttacgaaaaa atgctttccg ataaacagat
      601 cgatactgtt tatgtggcag tcagtaatca tatgcattat atatttgtaa aaaaagcgct
      661 gctttccgga aagcatgtta tttgtgaaaa gccatttacg gtcacgcttt cggaatttga
      721 tgaactatgc cgcattgccc gtgaaaaaaa gctttttttg ctggaagcca taacaacact
      781 ttattttccc aattatcttg caatcaaaga agctgtaaaa acgataggag atattcggat
      841 cattacatgt aatttttcac agtattccag ccgttatgat gactttaaaa aggggatcat
      901 aaagccttgt tttgatcctg ctatggcagg cggtgcgctg atggatctga atgtgtataa
      961 tatccatttt gtcgtgaatc tgttcgggga gccgaaaacc gtccggtatt atccgaatat
     1021 cagacaggga gtcgacgtgt cgggtatctt gatcctgtct tatgagacat ttcaatgtgt
     1081 actgattggg gcaaaggact gcggggcgcc gccctgtacg aatattcagg gagataaggg
     1141 ctgtatttat atggaatcgc cggcgaatac atgtgcgcgg ttcgaagtta tcctgaataa
     1201 ccaaaaacct gacaaaaaag aattgaataa agattttcac cggatgtatt atgaatttgc
     1261 agcattcgaa cggatgctta aggaaggcga tatggagact gcagctacaa tgcttgaaca
     1321 gagcagatgt gtcatgaaag ttctggagca ggcgaaagca ggcatgatta tttagagatt
     1381 tttaaagtcc taagatatgc cttttgttcg tcagtgatcc gataactttc gacagccttc
     1441 tgaatcgttt tattatgtgt ccatttttca agacgctgct ccgtaatgta tggaagaatg
     1501 gaatcatatt gtttggcaag tgcagtggca aagtaccagg caattatcat attgacataa
     1561 tactcttccg aacggatctg cgcgacaagt gcaggatata tgtcatcgaa attttcgtcg
     1621 agataaaaac tcatcagcat ttcgactgca aaacggacag tatatgtctg ctttgtattt
     1681 atccatgatt tgatctgttc tatcaaatgg ggcggatgtt ttttgaacgg cttcggagac
     1741 attaaatcac aggtggccca attgtcaaca taaggaagaa atgcatctaa tgatttgatg
     1801 catgtatcgt aatctgtgat ctcggatatg agaaaaccat ggaaattgtt ttcctcataa
     1861 tagcggtggg gaagtatctt taagaattcc gatacctccg gccgtttttt atattccttt
     1921 acaaggtttc taagcgccgg agtcctcacg ccaatgatcg tatcgggatc gatggtcggc
     1981 atcaggcggc tgtggaatgc tttgtatttg agatcctgca attcgaaaag ccgggtcctg
     2041 atctcctttt cgatttcttt ttcgggcata tgacagaatt ccttccggtt attttgtatt
     2101 gtaattcagc tttaccgtga tcgttgcggt cttgtctctg gaagaagtgt tgaaagtacc
     2161 gccatagctg tattcttcgg aagcagaatt ttgagcggtg atctgaaaga caccaaggct
     2221 ggcgcttgcc agagcgtatg ctttggagcc ggttccttca gcaataatgt cgatccgctc
     2281 ttttgcattt gcggtagcgt cttcaatcag ctgcagcttt agttcgtcaa gtttggtgta
     2341 ataatattcg ggtgcgtcgg aagtaaattc aataccggat tcgataagac ccgtgatatc
     2401 ccttgaaatt ttttcgacct tatccacatc tgatgaagta atacagacat cctgatacag
     2461 atcatagccg gccagttcgt cgccgacata attaccatct tcgttataaa gagaattgta
     2521 gtgtctgttg atattaacgg aactgaacac catctcgtct tccgtgatcc cattttcctc
     2581 gagatactcc ctgactgtct gtgtatcttc tttgatggtg cggtaggcgt ccgaggttgt
     2641 ttccccgaat gcggaaaaac tgccgcgcca tacgatcaga tcggattcaa aatcgcagct
     2701 tgccgaaccg gttgcggtaa taccgtaagc gcccgctttg cttttataat ttaaaaatcc
     2761 gaaagtcagg atcacagtac aggcaatgat actgatacct gcgatgcctg cgattatgat
     2821 cccgctgtga tttctaccgg ttttttcttt tttttcattt gataagatct gtttttcctg
     2881 ctcctgttcc atatatgcct ccccgctgcc ggcatagaat cgctttttgt ctgacatgcc
     2941 ggaccacaca actgataaaa gctgataaca cataaatata ctattacata attatcatat
     3001 ctgtttttaa taatactgtc aattacggcc tgatgaaaat attcgcggta attggttgaa
     3061 agaagattac ggataaagta taatcatgtt gtatttgaat gaaaacatgc cgcgccgctg
     3121 ggaaagcagg aacataaaat aaatattatg t
//
LOCUS       DAOSYE010000176         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22513_length_3150_cov_2.826171, whole genome shotgun sequence.
ACCESSION   DAOSYE010000176 DAOSYE010000000
VERSION     DAOSYE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22513_length_3150_cov_2.826171"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..570
                     /gene="rnhA"
                     /locus_tag="PLU43_04065"
     CDS             88..570
                     /gene="rnhA"
                     /locus_tag="PLU43_04065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOO27615.1"
                     /translation="MKVMIFTDGSARSNPDGPGGYGTILQYVDRKGELHEREYSAGYK
                     KTTNNRMELMAVITGLEALTKPCEAEVVSDSKYVTDAFNQHWIENWQKRNWKTSTKKP
                     VLNMDLWKRLLKAMEPHNVTFTWVKGHEGHPQNERCDKLATTAADKEGLLDDTGQEVL
                     "
     gene            651..809
                     /locus_tag="PLU43_04070"
     CDS             651..809
                     /locus_tag="PLU43_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium nitrogenase"
                     /protein_id="HOO27616.1"
                     /translation="MVAFVNSFLSYLLLAVVSMGLIALAVFLGKKLRDKKDEKDMLKE
                     TEENEHYV"
     gene            793..2280
                     /gene="thrC"
                     /locus_tag="PLU43_04075"
     CDS             793..2280
                     /gene="thrC"
                     /locus_tag="PLU43_04075"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO27617.1"
                     /translation="MSIMYKSTRGMQGEFPASVAIIKGLADDGGLFVPERIPKLSIEL
                     SALRGMSYQETAYHIMKEFLTDYTEEELKSCIEKAYDNKFDTPEIAPLVKRGDQYFLE
                     LFHGATIAFKDMALSILPHLLTIAMKKNKIDKEIVILTATSGDTGKAALAGFADVPGT
                     RIIVFYPKGGVSKIQELQMVTQKGKNTRVIGVHGNFDDCQTGVKNIFSDRELREEIGE
                     NGYQFSSANSINIGRLVPQIAYYAYAYGSMLRMEEIKEGDLLDVVVPTGNFGNILAAY
                     YAKQMGIPIGKLVCASNENKVLFDFFKTGSYDKNREFILTSSPSMDILISSNLERLIY
                     LLCNQDATRNAAFMKSLSETGSYEITAEMKEKLSDFYGAYATEQEVFSTIHEIYDRTG
                     YVIDTHTAVAAFADQVYKKDENTYNKSIIVSTASPFKFTRNVMKAVAPSYTDTDDFKM
                     VDELGRLAGVKVPAAIEEIRNAQVLHEQVCDAKSMRYAVKESLGM"
     gene            2284..>3150
                     /locus_tag="PLU43_04080"
     CDS             2284..>3150
                     /locus_tag="PLU43_04080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27618.1"
                     /translation="MSREKHILVVDDVATNLICASEVLRKKFRVTTARSGEAAFTAMQ
                     KERPDLVLLDIVMPEMNGYELYNRMKNDEQLADVPVIFLTGDTNNASEAQGISMGAMD
                     FLIKPFVPEVLISRIERALKLDSVKRGLEKQVELKSVEAYKDSLTGLWNRKYTEEKVN
                     AYLADELANGVLFILDMDNFKEVNDTYGHIRGDAVLIEFANILKSCTRDDDIVCRIGG
                     DEFIIFFKGNFDEKGASTKSNEIISKVEERFEKMLEIGENRIHVSVSIGITFAPQDGS
                     DFMTLYNNADKSM"
BASE COUNT          993 a          497 c          823 g          837 t
ORIGIN      
        1 gaaaaagcgg aaaatgctgc aggatatttt ttgaaaacag gcagctggga agcaatatca
       61 taaatggacg aaaaggagca gttaattatg aaggtaatga tttttacgga cggttcggcc
      121 agaagcaatc cggacggacc gggaggatac ggaacgattt tgcagtatgt agaccgtaaa
      181 ggcgaactgc acgagcggga atattcggca ggctataaaa agacaacaaa taacaggatg
      241 gagctgatgg cggtcataac aggactggag gcgctgacaa agccatgtga ggcagaagtc
      301 gtctccgatt caaaatacgt aacggatgcg ttcaaccagc attggataga gaactggcag
      361 aagcggaact ggaaaacgag tacgaaaaaa ccggttttga atatggatct ttggaaaaga
      421 ctgctgaagg caatggaacc tcataacgtg acgtttacat gggtcaaggg ccatgaagga
      481 catccgcaga atgaacgctg tgataaactg gcaacaacag cagccgacaa agaaggtttg
      541 cttgacgata ccggacagga agtgttatag taaaaatcgt ataatacatt attttatcag
      601 atattatatg gtggtagtta tgtatgaatt aaataaggac aggtgatatt atggtagcat
      661 ttgtaaattc ttttttatca tacctgcttt tagcggtcgt aagtatggga ctgattgctc
      721 tcgcagtgtt ccttgggaaa aaactgcgcg acaaaaaaga cgagaaggat atgctgaagg
      781 agacagaaga aaatgagcat tatgtataag agcacccggg gaatgcaggg ggaatttcct
      841 gcttctgttg cgatcataaa agggttggcc gatgacggcg gtctttttgt gccggaaaga
      901 attccgaagc tttcgatcga actttccgct ttgcggggta tgtcatatca ggaaacagcg
      961 tatcatatta tgaaagaatt tctgacggat tatacggagg aagaactgaa gagctgtata
     1021 gaaaaggcat atgataataa atttgataca ccagagattg cgccgcttgt gaaaagagga
     1081 gatcagtatt ttcttgaact gtttcatggg gcgacgattg catttaagga tatggcgctt
     1141 tctattctgc cgcatctttt aacaatagca atgaaaaaaa ataagatcga caaggaaatt
     1201 gtgatactga ctgcaacatc cggtgatacg gggaaggcgg cacttgcagg ctttgctgat
     1261 gttcccggta cgcggatcat cgtattttat ccgaagggcg gagtcagtaa gatccaggaa
     1321 cttcagatgg ttacacagaa aggaaaaaat accagagtta ttggtgttca tggtaatttt
     1381 gacgactgtc agaccggagt taaaaatatt ttcagtgaca gggaattaag agaagagatt
     1441 ggagaaaatg gttatcagtt ttcttccgca aattccataa atatcggacg cttggttcct
     1501 cagatcgcgt attacgcata tgcttatgga agtatgcttc gtatggaaga aataaaagag
     1561 ggcgatttac tggacgtggt cgttccaacc ggtaattttg ggaatattct ggcagcttat
     1621 tatgcgaaac agatgggaat tccgatcgga aagcttgtct gtgcttcaaa cgaaaataaa
     1681 gtattgtttg atttttttaa aacaggcagc tatgataaaa acagggaatt tattctgaca
     1741 tcgtctccgt cgatggatat tttgatttcc agtaatttgg aacgtttgat ttatctgctt
     1801 tgtaatcagg atgccacaag gaatgctgct tttatgaaga gcttgtccga aacgggcagt
     1861 tatgagatta cagcggaaat gaaagaaaaa cttagtgatt tttatggggc ctatgcaact
     1921 gagcaggaag ttttttcgac gatccatgag atttatgatc gtacgggtta tgtcattgat
     1981 acacataccg cagttgcagc ctttgcggat caggtgtata agaaagatga aaacacctac
     2041 aataaaagca ttattgtttc aacagcaagt ccgtttaagt ttacgcggaa tgttatgaaa
     2101 gcagttgccc cttcttatac ggatacggat gattttaaga tggtggatga gcttggcagg
     2161 ctcgctggtg tgaaggtgcc tgctgcaata gaagaaatca ggaatgcgca ggttttgcat
     2221 gagcaggtct gtgatgcaaa gagtatgcgg tatgcagtga aggaaagctt gggaatgtaa
     2281 caaatgagca gggaaaaaca tattttggtt gtagatgatg tggctacaaa tttaatatgc
     2341 gcaagcgaag tactcagaaa gaaattcagg gtcacgacgg cacgttccgg tgaagcggca
     2401 tttactgcca tgcagaaaga acggccggat cttgttttgc tggatattgt gatgcctgaa
     2461 atgaatgggt atgaacttta caacagaatg aaaaatgatg agcagcttgc tgatgttccg
     2521 gtaatttttc tgaccgggga tacgaataat gcaagtgagg cgcaggggat cagcatggga
     2581 gcaatggatt ttctaataaa gccatttgtt ccggaggtcc ttatctccag gatcgagcgg
     2641 gcgcttaagc ttgattcggt taaacgcgga ctggaaaaac aggtggaact gaaatctgtc
     2701 gaggcgtata aggactctct gaccggtctt tggaaccgta aatatacgga agaaaaagta
     2761 aatgcatatc ttgcggatga gctggcaaat ggtgtactgt ttattcttga tatggataac
     2821 tttaaagaag tgaatgatac atacggacat atcaggggag atgccgttct gattgagttc
     2881 gccaatattt taaagagctg tacaagggat gacgatattg tttgtcgtat tggcggggat
     2941 gagttcatca ttttctttaa agggaatttt gacgaaaaag gagcatcgac aaaatcaaac
     3001 gaaatcattt caaaagttga agaacggttt gaaaagatgc ttgaaatagg ggaaaacaga
     3061 attcatgttt cggtttccat tggaattacg tttgcaccgc aggatggcag tgattttatg
     3121 acattatata ataatgcgga caaatcaatg
//
LOCUS       DAOSYE010000177         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22660_length_3138_cov_3.148881, whole genome shotgun sequence.
ACCESSION   DAOSYE010000177 DAOSYE010000000
VERSION     DAOSYE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22660_length_3138_cov_3.148881"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..983)
                     /locus_tag="PLU43_04085"
     CDS             complement(51..983)
                     /locus_tag="PLU43_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO27619.1"
                     /translation="MFSKVNERGFGYVYLIDRNGELIYHPEQKAIYSGLVQENNKKAA
                     LYEDGDYIETFMGEERAVVVKTVGYTGWKIVSVTPTKEFSMNMMQLKFFMLMVCTVTI
                     FLILFGNIFISDRVTNPIRKLEASIKYLEEGNLDTDKIYIGGSHEIRHLGRTIRSMVN
                     QMRKLMDEMVKEQKAKRKSELDALQSQINPHFLYNTLDSVVWMIESERYKEAISMVRA
                     LATLFRISLSKGSNIISIKDEITHAKNYLSIQQVRYKNKFTVTVDIAPEIENCSTIKL
                     IIQPLLENAIYYGVEHMDGDGEITLRGYEKDGDI"
     gene            complement(984..1613)
                     /locus_tag="PLU43_04090"
     CDS             complement(984..1613)
                     /locus_tag="PLU43_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO27620.1"
                     /translation="MKERLFKLVNRFKNAYKKRSIQLIISMSFTFVAILSMTFMGIAF
                     YTHFVNSSKESAIENNKQILDQVSWNLNAYIRNMMSISDSMYYNVIKNKDLTYDTIDK
                     EMNLLYEANKDNLISIACVSEDGALISASPVATRKVDVDFKEQEWFKQAENKIENLHF
                     STPHVQDMFESSNYRYYWVVSLSRSVELTNVGHIRRGVLLVDMNFSAIE"
     gene            complement(1610..>3138)
                     /locus_tag="PLU43_04095"
     CDS             complement(1610..>3138)
                     /locus_tag="PLU43_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27621.1"
                     /translation="VVASAENGEDALEKAEQYLPDVVMSDIHMPFMDGLTFCRRLKEQ
                     QPGTKIVIFSGYDEFEYAKEAIKLEAEEYILKPVDSEELKKVFIRIKESLDEERDKRR
                     NIERLEKYYQESIPILKEQLLIGILEGRVSEEDMIGYQQDYRLSMDAAFYAVGIVKPD
                     YSEEPDLKKDGLRGNLISVSLKQLADEELEKMIDFKSINYLGIIVVVAMLKNTASHKN
                     FMTIMDQICKLSYKMLGINTIAGIGKIYGKLQDISYSFSEAKDAASYRILFDTNQAIY
                     IQDVDPKSDDNYFMDEKQIQQIIKEIKIGSKENLKEAVEFLIRRMKNTAITPAQLQLF
                     FTEIFVEIMRLARGYQLTSQQMNLPDMDIYNEIKNFNSLDDLGSWLLNICMRLRSSIR
                     RERTDTAKLLTEKAKQYIADNYMDSALSVDKVCGHLNVSATYFSALFKKDTGISFVAY
                     LTQLRMEQARRLLDTTEEKSYIIAGMVGYEEPNYFSYVFKKQFGVSPSKYRQKQEKTG
                     "
BASE COUNT          822 a          778 c          544 g          994 t
ORIGIN      
        1 actaatcaat acattacgtg gtggcaatcc cacgagaaaa atgattgacc ctaaatatct
       61 ccgtcctttt catagccgcg cagcgtgatc tcaccgtccc cgtccatatg ctcgacgcca
      121 taatagatcg cattttccaa aagcggctgg atgatcagct tgatcgtaga acagttttca
      181 atttccggtg caatatcaac tgtgaccgta aacttatttt tatagcgtac ctgctgaata
      241 ctcagataat ttttggcatg cgtgatctca tctttgatcg aaataatatt gctgcccttg
      301 ctcagactga tacggaacag tgtcgccagc gctcttacca tggagatcgc ttctttatag
      361 cgttcgcttt cgatcatcca gacaacggaa tccagtgtat tataaagaaa atgcgggttg
      421 atctgtgact gcagcgcatc cagttcgctt ttccgttttg ccttctgctc ctttaccatc
      481 tcatccatca gtttacgcat ctgatttacc atggatctga tcgttctgcc aagatgcctg
      541 atctcatggg aacctccgat atagatctta tccgtatcaa ggtttccttc ctccagatat
      601 ttaatggatg cctccagttt ccggatcgga ttcgttactc tgtccgaaat aaaaatattg
      661 ccgaataaga tcagaaaaat agtcacagta cagaccatca gcatgaaaaa cttaagctgc
      721 atcatattca tggaaaattc ctttgtcggc gttacactga cgatcttcca gccggtataa
      781 ccgaccgtct ttacgacaac tgcacgctcc tcgcccataa aggtctcaat atagtcccca
      841 tcctcatata atgctgcttt tttgttgttt tcctgtacca ggccggaata gatcgctttc
      901 tgctccggat ggtagatcag ttccccattg cggtctatca ggtaaacata cccgaaccct
      961 ctttcattga cctttgaaaa catctactct atcgcactga aattcatatc aacaagcagg
     1021 actcctcttc ggatatgccc tacatttgtc agctccacac tccggcttaa ggacacgacc
     1081 cagtagtacc ggtaattact gctttcaaac atatcctgca catgcggcgt ggaaaaatgc
     1141 agattctcta ttttattttc cgcctgctta aaccactcct gttctttaaa gtcaacatcg
     1201 acttttcttg tcgcaacggg tgaagcggaa atcagggccc cgtcctcgga cacgcaggca
     1261 atgctgatca gattatcttt atttgcctca taaagcagat tcatttcctt atcgatcgta
     1321 tcataggtca gatctttgtt tttaatgaca ttataataca tggaatcgga aatgctcatc
     1381 atattacgga tatacgcatt cagattccag ctcacctgat ccagtatctg tttgttgttt
     1441 tcaattgccg attccttcga gctgttgaca aaatgagtat aaaaagcaat ccccataaat
     1501 gtcatgctca aaatggcgac gaacgtaaac gacatggaaa tgatcagctg gatacttctt
     1561 ttcttatatg catttttaaa tctgtttacg agcttgaaaa gcctttcttt catcctgttt
     1621 tttcctgttt ttgtctgtac ttggacggtg aaacgccaaa ctgcttctta aacacataac
     1681 tgaaataatt cggttcttca taaccaacca tgccggcgat aatataactt ttctcttctg
     1741 tcgtatcgag cagacgtctg gcctgttcca tgcgcagctg tgtcagatag gcaacaaaac
     1801 ttattcccgt atccttttta aacagtgcgg aaaaataagt cgcactgaca ttcaggtgtc
     1861 cgcagacttt atctaccgac aatgcggaat ccatataatt gtcggcaata tattgttttg
     1921 ccttctctgt cagcagcttt gcggtatctg ttcgctccct gcgaatactg cttcttaacc
     1981 gcatacagat attgagcagc cagcttccaa ggtcatccag agaattaaaa ttcttgattt
     2041 cgttataaat atccatatcg ggcagattca tctgctgtga agtcagctga tagcctcttg
     2101 ccagccgcat gatctccaca aagatctccg taaaaaacaa ttgcagctgt gccggcgtga
     2161 tcgctgtgtt tttcattctc ctgatcagga attccactgc ttctttcaga ttctcctttg
     2221 aaccgatctt aatttccttg atgatctgct ggatctgctt ttcatccata aaataattat
     2281 cgtccgactt tggatccaca tcctgaatgt aaattgcctg atttgtatca aacaagattc
     2341 gatatgacgc cgcatccttt gcctctgaaa aggagtagga aatatcctgc agttttccgt
     2401 atatttttcc gattcctgca atcgtattga ttccaagcat tttataggac aatttacaga
     2461 tctgatccat gatcgtcata aaatttttat gggatgcagt attttttaac attgcaacga
     2521 caacgatgat tccaagataa ttgatgcttt tgaaatcaat catcttttcc agctcttcat
     2581 ccgcaagctg tttcagcgat acggaaatca gattgcctct tagaccgtct ttttttaaat
     2641 ccggttcttc cgaataatca ggctttacaa ttccaacagc ataaaatgcc gcatccatcg
     2701 aaagcctgta atcctgctga tatccaatca tatcttcttc ggatacccgt ccttccaaaa
     2761 ttccgatcag cagctgttct tttaagatcg gaatgctttc ctgataatac ttttccagac
     2821 gctcaatatt ccttctctta tccctctcct catccagaga ctcctttatc ctgataaaaa
     2881 ccttttttaa ttcttcggaa tcgaccggtt ttaaaatata ttcttctgcc tccagcttaa
     2941 tggcttcttt tgcatattca aattcatcat aacccgaaaa gatcacgatc tttgttcccg
     3001 gctgctgctc tttcaggcgt ctgcaaaaag tcaggccatc cataaacggc atatgaatat
     3061 ccgacatgac gacatccggc agatattgtt ctgctttttc cagcgcgtcc tccccgtttt
     3121 ccgcagaagc cacaactt
//
LOCUS       DAOSYE010000178         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22675_length_3136_cov_2.860759, whole genome shotgun sequence.
ACCESSION   DAOSYE010000178 DAOSYE010000000
VERSION     DAOSYE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22675_length_3136_cov_2.860759"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLU43_04100"
     CDS             <1..562
                     /locus_tag="PLU43_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO27622.1"
                     /translation="WGLEKDLLLPFKQQTKKVNAGEECLVALYIDKSGRLCLTMNVYE
                     YLRTDSDYQAGDKVEGRVYLISEQFGAFVAVDDCYSALIPRKELYGDIRINHRITGRV
                     TEKKEDGKLTLSIREKAYIQMDQDAGRVMQVIEAFDGVLPFNDKASPEVIKREFNMSK
                     NEFKRAVGKLYKERLIEITEKSIRKK"
     gene            646..1023
                     /locus_tag="PLU43_04105"
     CDS             646..1023
                     /locus_tag="PLU43_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO27623.1"
                     /translation="MEKHVISTKKAPAAIGPYSQAIEVNGMIYTSGMIPVIPETGEIV
                     EGIEAQARQALSNVKALLESQGSSMEHVIKTTVFIKNMDDFSKVNEVYATFFNKEFPA
                     RSCVEVARLPKDVLIELEAIALK"
     gene            1048..1281
                     /locus_tag="PLU43_04110"
     CDS             1048..1281
                     /locus_tag="PLU43_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HOO27624.1"
                     /translation="MKVQNITDIDKFFKVIDSCKGKVELVTGEGDRLNLKSKLSQYVS
                     MANIFSDGTIEELEIIAYEPEDIDKLVSFKING"
     gene            1376..2269
                     /locus_tag="PLU43_04115"
     CDS             1376..2269
                     /locus_tag="PLU43_04115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO27625.1"
                     /translation="MLLLRSLSVPPAVQFIASELIILIPGFLFLLIYNCDLRKWIPFK
                     KMNGYSIGMTVLFTYLMMPLISFINIFSQLFSENEILGITDEILSIPSLLVVFIIGIL
                     GPFCEEFIFRGIIFSGYKKTGRLLGALILSSLLFGLMHMNFNQFCYAGILGFVFALLV
                     EATGSLWSSVISHMIVNTHNVVMLFAVQKLYTALGTDMEQLYGESVTLDEKLYALGLF
                     LILSVIFTSLAAAVFAAICKRQGTAGHIRDIFKSRQENLTGPESRRKKEPLLTFSGVF
                     GIAVCLFVMFGLDPVLRMLGW"
     gene            complement(2346..2522)
                     /locus_tag="PLU43_04120"
     CDS             complement(2346..2522)
                     /locus_tag="PLU43_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HOO27626.1"
                     /translation="MDIPALSMVMAQSKVAADFSTGMLSKSLDTFETAGDDLSKMMEL
                     SVNPEIGANIDLQV"
     gene            complement(2591..>3136)
                     /locus_tag="PLU43_04125"
     CDS             complement(2591..>3136)
                     /locus_tag="PLU43_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27627.1"
                     /translation="FLCFLTYYGVLLFGTIVSKLMTANIFVDRYLFFASGLIWLFFAI
                     EAGSLRKDFIYIILVFEIFIGVIGYYQEYRLEYAEGIDTLKTYLDASVEKNDILYTLE
                     DYEELAYCLPFYEQTLTNYEDLSEAVKAADSTQGTIWCAVMDEYEYDESLFDSYGLKM
                     EFVGEFRFDRYQFKLYKLIKK"
BASE COUNT          929 a          568 c          731 g          908 t
ORIGIN      
        1 ctggggtctg gaaaaagatc tgcttttacc gtttaagcag cagacaaaaa aagtaaacgc
       61 cggcgaagaa tgcctggttg cattatatat tgataaaagc ggcagactgt gtctgacgat
      121 gaatgtatat gaatatctgc gtacggattc ggattatcag gccggggata aagtggaagg
      181 cagggtttat ctgatcagcg agcagtttgg cgcttttgtg gcagtggatg actgttattc
      241 cgcattgatt ccgaggaaag aactgtatgg agacatcagg atcaaccaca ggatcacagg
      301 cagggtgacg gaaaagaaag aagacggcaa gctgacgctt agtattcgtg aaaaagcata
      361 tattcagatg gatcaggatg ccgggcgtgt catgcaggtg atcgaagcgt ttgacggtgt
      421 actgccgttt aatgataaag cttctccgga agtgatcaaa cgggaattca atatgagtaa
      481 gaatgaattc aagcgtgcgg taggaaaatt atataaggag cggctgatcg agattacgga
      541 aaaatccatc cgtaagaaat gagaacattg gattcgtgat ttttttattt acaattcgga
      601 caaaaatgta taaactagtg ttagaaacag aaggaggcaa ttcgaatgga aaagcatgta
      661 atcagcacaa aaaaggctcc ggcagcaatc ggtccttatt cacaggcaat tgaggtaaac
      721 ggtatgatct atacgtcggg tatgatcccg gtcattccgg agacaggtga aatcgtagag
      781 ggtatcgagg cgcaggcaag gcaggcactt tccaatgtga aagcattgtt ggagtcacag
      841 ggaagcagca tggagcatgt gatcaagacg acggtattta ttaaaaatat ggatgacttt
      901 tcaaaggtaa atgaagttta cgctactttt tttaataaag aatttcccgc aagaagctgt
      961 gtggaggttg cgcgtctgcc gaaagacgtt cttatcgaat tggaagcaat tgcattaaaa
     1021 taaagacaaa atgaaaggag agagataatg aaagtacaga acattacgga tattgacaaa
     1081 tttttcaagg tgatcgacag ctgcaagggg aaggtggagc tggttactgg tgaaggcgac
     1141 agactgaatt tgaaatccaa gctgtcccag tatgtttcaa tggcaaatat tttttccgat
     1201 ggtacgatcg aggaacttga gatcatcgca tacgaaccgg aagatattga caagctggta
     1261 tcttttaaga taaacggtta aggaacggct gcaaatgata cggatatctg gaaaaataaa
     1321 cagacttttt ttgtggatag taattttgca tctgacagca tcgataatat tatcgatgct
     1381 gttattacgc tcgctttcag ttcctcctgc cgtacagttt atagccagtg aactgatcat
     1441 cttgattccg ggttttctgt ttctgcttat ttacaactgt gatctgcgaa aatggattcc
     1501 attcaaaaaa atgaacggct attccattgg gatgacggtt ttatttactt acctgatgat
     1561 gccccttatt tcttttatca atattttttc acagctgttt tcggaaaatg agatacttgg
     1621 tattacagat gaaatacttt ccattccttc tcttctggtt gtttttatta ttgggatatt
     1681 gggtccgttt tgtgaagaat ttatattccg cggaattata ttcagcggtt ataaaaaaac
     1741 cggcagactg cttggcgcgc tgatactttc ttctcttttg tttggcctga tgcatatgaa
     1801 tttcaatcag ttctgttatg ccgggatact tggttttgtt tttgcccttc ttgtagaagc
     1861 gacaggcagt ctctggtctt ccgtgatcag tcatatgatt gtcaacacac ataatgttgt
     1921 gatgctgttt gctgtacaaa agctttatac ggcgcttgga accgatatgg aacagctata
     1981 tggagaaagt gtgactttag atgaaaaatt gtatgcgctc ggcttatttt tgattttatc
     2041 tgtcattttc acttcgcttg cggctgcagt ttttgctgca atatgcaaaa gacaaggaac
     2101 ggccggacat atacgtgata tttttaaaag caggcaggag aacctgacgg gaccggaaag
     2161 cagaaggaag aaagaaccgc tgctgacatt cagcggtgta tttggaattg cagtatgctt
     2221 atttgtaatg tttggcctgg atccggtgct gcgtatgctt ggatggtagg acattcattt
     2281 ttcggcataa aatatgaggc ggagaaatca tttctccgcc tcgtttgatg acagcatctg
     2341 cagaatcaga cctgcagatc aatgttggca ccgatttcag gatttacgga cagttccatc
     2401 atttttgaca agtcatcccc ggccgtttca aaggtatcca aagacttgct cagcataccc
     2461 gtactgaagt ccgcggcaac ctttgactgt gccattacca ttgataatgc gggtatatcc
     2521 atttgatcag tccccctttc cgtgcctgct gaaaataaaa gcctgcataa ataatatcgg
     2581 catatacgtg ttatttctta attaatttat ataacttaaa ttgatacctg tcaaatctga
     2641 attctccgac aaactccatt ttcaatccgt aagaatcaaa tagggattca tcatattcat
     2701 attcgtccat aacggcgcac cagattgttc cctgcgtgct gtcggcagct tttactgcct
     2761 cggaaagatc ttcatagttt gtgagtgtct gttcataaaa agggaggcaa taagcgagtt
     2821 cttcgtaatc ctcaagagtg taaaggatat catttttttc aacgctggca tccagatatg
     2881 ttttcagcgt gtcgatccct tcggcatatt ctaggcgata ttcctgataa tagccgatca
     2941 caccgatgaa tatttcgaat acaaggataa tatagataaa atcttttcga agggagcctg
     3001 cttcgatcgc aaaaaacagc cagatcagtc cggatgcaaa aaacagatag cggtcgacaa
     3061 aaatatttgc ggtcatcagc ttcgaaacga ttgtaccaaa cagaaggaca ccataatagg
     3121 taaggaagca taaaaa
//
LOCUS       DAOSYE010000179         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22676_length_3136_cov_2.705940, whole genome shotgun sequence.
ACCESSION   DAOSYE010000179 DAOSYE010000000
VERSION     DAOSYE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22676_length_3136_cov_2.705940"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1273
                     /locus_tag="PLU43_04130"
     CDS             56..1273
                     /locus_tag="PLU43_04130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO27628.1"
                     /translation="MKKKEQTDDKNLSLSKQKRLERQKKQKKHKQKILIWKIAGTVLA
                     ALVAAGIIFLIVQQIYKAYSKVTPSSEFSAQLTDDGFIEDVTASSQLTLCDYKNITVP
                     LDEVEYTDDEMEQDIQNTLDQHQTLNTESTAEIADGDKVNIDYVGTVDGVEFDGGNSN
                     GEGYDLTIGSGTFIDDFEQQLIGHVVGDNVTVDVTFPDDYSNDTSLAGKDASFAVTIN
                     GIYESPAFDDDFVKENLSDYADSADGYRQYLKDTNYQSNLDTWIAQYLVDHTTVKHYP
                     KAYLKNLKATQKYSDQNSYEYMNQLYTSYYGSSVYDSFEDYVGMSEEEYDISLNDTCK
                     SIEKEAMVYQAILETEGLTVTEDDYKAYLTETNGSSDSYDTQVSQYGKGYAMQTMVRV
                     KALEIVEEYAVVE"
     gene            complement(1347..2060)
                     /locus_tag="PLU43_04135"
     CDS             complement(1347..2060)
                     /locus_tag="PLU43_04135"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009982724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOO27629.1"
                     /translation="MNILISIATCVLIGYLFGCINLSYLFSKVKGFDIRNFGSQNAGA
                     SNVVILMGKKTGAIVAASDILKAYLAVKLSIWLFPEFFLAGSVAASVVILGHIFPFYM
                     GFRGGKGFASLGGAVLALDYRMFLVLFVVALFIALVTDYICFAPISIALIFPTAYGFV
                     NGSVPAAFILYISSVFIWYRHMGNLKRIKNGRELKFSFLWNRKKEAERFGVSDDDGKD
                     YPLYMDSQHAEESDLHKTR"
     gene            complement(2189..>3136)
                     /locus_tag="PLU43_04140"
     CDS             complement(2189..>3136)
                     /locus_tag="PLU43_04140"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOO27630.1"
                     /translation="PSNPILTHRHLGKEYPIRYVGHADLVNVPAEMTLSGEEKWYMVC
                     LASRPYQGHTMLGRETFLAEVVWEDGWPVVNPGKGKLTKEAVIDLPESEIGEQDSSIT
                     FHCDKLPDKMLMLRNPGGDAYSLQAHKGCLRLPLKNSSLKSYESPAFAGVRQTAFYFE
                     ASTAFQFFPENETEAAGLVIFQSNQFHLKLQIVKRKEYAVAQAVFVKALTADGMINGD
                     VKKLPVTEELLFEEKICKIKESNCYRIRISCDGIKVSAFADSDDSGIMEKRFAKKIPI
                     EALSTEAAGGFVGNLIGLFAESEEQESRNHADFLFFDIL"
BASE COUNT          987 a          735 c          619 g          795 t
ORIGIN      
        1 agacgccagc tgataggctg acaacaaata agacaggaaa caggaagagg tcattatgaa
       61 aaagaaagaa cagacagatg acaaaaactt gagcttgagc aagcaaaaga gactcgaaag
      121 acagaaaaaa caaaaaaaac ataaacaaaa aatactgatt tggaaaattg ccggcaccgt
      181 tcttgcggca ctggttgcgg ccggtatcat ttttctgatc gtacagcaga tttacaaagc
      241 ttacagtaaa gtaactccaa gcagtgaatt cagcgcccag ctgaccgatg acggatttat
      301 cgaggatgtg actgcatctt cacagctcac cctctgcgat tataaaaaca ttaccgttcc
      361 gcttgacgaa gtcgaatata cggatgacga gatggagcag gatattcaga atacgctcga
      421 ccagcatcag actctgaaca cagaaagcac tgccgagatc gcagacggcg ataaagtcaa
      481 tatcgattat gtcggcaccg ttgacggtgt ggaattcgac ggcggtaaca gcaatggaga
      541 gggatatgac cttacgatcg gatccggtac tttcatagat gattttgagc agcagctcat
      601 cggacatgtt gtcggagaca atgtaactgt ggatgtaaca ttcccggatg attattccaa
      661 tgatacttca ctggccggta aagatgcttc ttttgcagtt accataaacg gtatttacga
      721 atctccggca tttgacgatg acttcgttaa agaaaatctt tcagactatg ccgattcggc
      781 agacggttac cgtcaatatc tgaaagatac aaattatcag tccaacctgg atacatggat
      841 cgcgcaatac ctggtcgatc atacgaccgt taagcattat ccgaaggctt atctgaaaaa
      901 tctgaaagcc acgcagaaat acagcgacca gaattcctat gaatatatga atcagcttta
      961 tacaagctat tacggaagca gcgtatacga cagttttgaa gattatgtcg gcatgtcgga
     1021 agaagagtat gatatcagtc tgaacgacac atgcaagtcc attgaaaagg aagccatggt
     1081 ctatcaggca attcttgaaa cagaaggact cacggttacg gaagacgact ataaggcata
     1141 tctcacagag acaaacggct cttccgattc ctacgataca caggtatcgc aatacggaaa
     1201 gggttatgca atgcagacaa tggtgcgtgt caaggcactc gaaattgttg aggaatatgc
     1261 agtagtggaa taaaccttac tataggatat aaaaacagac gattcattaa acgaatcgtc
     1321 tgttttatat cgatgacaac cgtagatcac cgggtcttat gcagatcgct ctcttcggca
     1381 tgctgcgaat ccatgtaaag cggataatcc ttgccgtcgt catccgaaac accaaaccgc
     1441 tccgcttctt tctttctgtt ccataaaaag ctgaatttca attccctgcc atttttaatt
     1501 ctctttaaat ttcccatatg gcgataccag ataaaaacag atgaaatata taatatgaac
     1561 gccgccggaa cagatccgtt cacaaaccca taggccgtcg gaaaaatcag ggcaatactg
     1621 atcggtgcaa aacagatata atctgtgacc agggcaataa acaaagcaac aacaaacagc
     1681 accaaaaaca tcctgtaatc aagcgcaagc actgcgcctc ccagagaagc aaatcccttt
     1741 ccgccccgaa agcccatata gaacgggaaa atatgtccca aaataacaac cgacgctgca
     1801 actgaaccag ccagaaaaaa ttccgggaat aaccagatcg ataacttcac agccagatat
     1861 gcctttaaaa tatctgatgc tgcaacaatc gcacctgtct ttttccccat caaaataaca
     1921 acattggaag caccggcatt ttgtgaccca aaatttctga tatcaaaccc tttcactttt
     1981 gaaaaaagat aagataaatt aatacagcca aataaataac caatcagcac gcatgtagca
     2041 attgaaatta aaatattcat ccatacccct gcttttccta tactgtccgt atcaacatga
     2101 cagatacctt aaatacatta tcacgtaaag tataccgcaa atttttttca ttttcaacaa
     2161 tacagcgcaa agtgattgaa tatatcatct ataaaatatc aaaaaacaga aaatcagcgt
     2221 gatttcggct ttcctgttct tccgactctg caaacagacc gatcagattc cctacgaatc
     2281 cgcctgccgc ctctgtgctg agcgcttcga tgggaatttt tttcgcaaag cgtttttcca
     2341 taatgccgga atcgtcagaa tctgcaaacg cactgacttt tattccgtca cagcttattc
     2401 tgatccggta acagttcgat tccttaattt tgcatatctt ttcttcaaac agcagttctt
     2461 ccgtaaccgg aagttttttc acatctccat tgatcatacc gtccgccgtg agggctttta
     2521 caaataccgc ctgtgcaacg gcatattctt ttctttttac gatctgcagc ttcaaatgaa
     2581 actgattgct ttgaaaaata acaagtcctg cggcctccgt ttcattttcc ggaaagaatt
     2641 ggaatgccgt cgatgcttca aaataaaaag cagtctgtct gactcctgca aaggccggcg
     2701 attcatagga tttcagggaa ctgtttttta aaggcaggcg aagacagcct ttatgtgcct
     2761 gcagggagta agcatccccg ccaggatttc gcagcattaa cattttgtcc ggcagcttat
     2821 cgcagtgaaa tgtaatagaa ctgtcctgtt cccctatttc agactccggc agatcgatca
     2881 ctgcttcctt ggtcagcttc ccttttccag gattcacgac aggccagccg tcttcccata
     2941 caacttctgc caggaaggtt tcccttccaa gcattgtatg gccctgatac ggccttgacg
     3001 ccaaacatac catataccat ttttcttcac cggaaagcgt catttcagcc ggtacattca
     3061 caagatccgc gtgcccgaca taacggatcg gatattcttt tccaagatgc ctgtgcgtca
     3121 ggatcggatt ggacgg
//
LOCUS       DAOSYE010000180         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22721_length_3131_cov_3.425228, whole genome shotgun sequence.
ACCESSION   DAOSYE010000180 DAOSYE010000000
VERSION     DAOSYE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22721_length_3131_cov_3.425228"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLU43_04145"
     CDS             complement(<1..1172)
                     /locus_tag="PLU43_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOO27631.1"
                     /translation="MKKTYYPTWESVKTHQVPEWYHDCKLGIFIHWGLYSVPAFATPT
                     VELGVVANEEWFCNNPYAEWYYNSLNMGSGATYEYHRKKYGEDFEYEAFADMWKAEKF
                     VPQEWASLFQQAGAGYVVMVTKHHDGFCLFPSKYTDYSAVNRGPQRDLVGELTEAVRE
                     KGLKMGLYYSGMIDWRFADDPIYTEDQVKFNCSPTYEYADYAYKQSLELIDNYHPSVF
                     WNDIGWPFAGERQLPYLLSHYYNVCEDGVVDDRFNDLWKDFSTKEYQYGKVTREEKWE
                     MCRGLGLSFGYNAVESDEHILSVGKLVSLLVSTVANNGNLLINIGPKADGTIPEIQAE
                     KLRGLGSWLAKNGEAVYGTRCSKRTSISGEEADIHFTRKNKDLYVILDGLREGDTAV"
     gene            complement(1272..2237)
                     /locus_tag="PLU43_04150"
     CDS             complement(1272..2237)
                     /locus_tag="PLU43_04150"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOO27632.1"
                     /translation="MIRNQWYAILPSRIVKTNQIVGIKRLNLELAIFRNNKGELGCVV
                     DQCTHRGAALSKGKVKGDCVQCPFHGLEFDAKGRCVFIPADGKASTADISRYHVRHYP
                     VLERNGIIYVWYGDQEKATEGLPFFESEIDASYAYSEIEDHWNAHYSRCIENQLDVVH
                     LPFVHYNTIGRGNRTLVNGPKIEFVPGGLVTSSNNEADIGQSPKTAAECVIKPTYLKF
                     LFPNIWMNHISDTVKVMIYFAPVDDENTILYIRFYCRVTGFKPLDSLIAHLGKIGNRI
                     IERQDKRVVITQKPKVSAYRSGEKLLSGDGPIIQYRRIRDELKNK"
     gene            complement(2361..>3131)
                     /locus_tag="PLU43_04155"
     CDS             complement(2361..>3131)
                     /locus_tag="PLU43_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO27633.1"
                     /translation="PIFFNDDVAIGIADTEKYLKIQQGKELVLKIEEGQPVPSGGAVY
                     NALKTGKTIIQEVPKAVYGIAFQSYAVPIKEDDQVIGVFVVGKSLKRKYEVLNTAETL
                     SGSLTQISDAVNVISDSVQSLAKNNNNIFEASKIAAEKTKDTDQVISFVKEIASETNL
                     LGLNAAIEAARVGENGRGFAIVAQEIRKLSTSTNESITKIDSVLSDVEHMVEKISNDL
                     KDTNDIVQTQAASLEEMAASIEELSATAEILKDISSTL"
BASE COUNT          816 a          811 c          538 g          966 t
ORIGIN      
        1 acggcagtat cgccttcccg cagtccatcc aggatcacat acagatcttt atttttcctt
       61 gtaaaatgaa tatctgcctc ttccccgctg atcgaagtcc ttttgctgca tcttgttccg
      121 taaactgcct cgccgttttt tgcaagccag ctgccaagac cgcgcagctt ctcagcctga
      181 atttccggta tggtaccatc cgccttaggc cctatattga tcagcagatt tccgttattg
      241 gctacggtag aaacgagtaa actcactaat tttccaaccg acagtatgtg ctcatcactt
      301 tccaccgcat tatatccgaa ggacagtcca aggccgcggc acatttccca tttttcttct
      361 ctggtcacct ttccatactg atattccttt gtactgaaat ccttccacag atcattaaag
      421 cggtcatcca ccacaccatc ttcacaaaca ttataataat gtgaaagcag atatggcagc
      481 tgcctctctc ctgcaaaagg ccagccgata tcattccaaa aaacggacgg atgatagtta
      541 tcgatcagct ctaacgactg cttatatgca taatccgcat actcataagt cggggaacag
      601 ttgaacttca cctggtcttc cgtataaatg ggatcatcgg caaatctcca gtcaatcatc
      661 cccgaataat acaatcccat tttcaatccc ttttcacgaa ctgcttctgt cagctccccg
      721 accagatcgc gctgcggtcc gcggtttaca gcgctatagt ccgtatattt gctcgggaaa
      781 agacaaaatc cgtcatgatg ctttgttacc attaccacat agccggcacc ggcctgctga
      841 aacagggaag cccactcctg cggcacaaat ttttctgctt tccacatgtc cgcaaacgct
      901 tcatattcaa aatcttcccc gtactttttc cgatgatact cgtaagtagc accgcttccc
      961 atgttcagtg aattgtaata ccattccgca tagggattat tacagaacca ttcctcattc
     1021 gccactacac ccaattctac tgtcggcgtc gcaaacgcag gcacagagta caggccccag
     1081 tggataaaaa tccctaattt acaatcatga taccactcgg gcacctgatg cgttttaacc
     1141 gattcccatg ttgggtaata cgtttttttc ataagcttcc ttctccttca tctttctgtc
     1201 ttgccgttta tttttctatc attttaagtt cttgccacag caatgtcaat gaaatgattt
     1261 ttctgtcatt ttcacttgtt tttcagttcg tcacgaattc ttctgtactg aatgatcggt
     1321 ccgtcaccgg aaagcagttt ctcccctgat ctatacgcag acacttttgg tttctgtgtg
     1381 atgacaacac gcttgtcctg tctttcaatg attcgattcc ctattttgcc caaatgcgca
     1441 atcagactgt ccaggggctt aaagcctgtc actctgcagt aaaaacgaat gtacagtatt
     1501 gtattttcat cgtcaacagg cgcaaaataa atcatgactt tcaccgtatc actgatatgg
     1561 ttcatccaga tgtttggaaa aaggaatttc aaataggttg gctttatcac acattctgca
     1621 gccgtctttg gactttggcc aatgtccgcc tcattattgg aactggtaac aagaccaccc
     1681 ggcacaaatt cgatcttcgg cccatttaca agtgtccgat tgcctctgcc aattgtattg
     1741 taatgcacaa aaggcagatg aacaacatct agctgattct caatgcagcg ggaatagtgg
     1801 gcattccaat gatcttctat ctcactgtaa gcataggaag catcaatctc cgactcaaaa
     1861 aagggcagtc cctccgttgc tttttcctga tcaccatacc atacatagat gatcccattg
     1921 cgttccaaaa ccggataatg cctgacatga taacggctta tatcggctgt ggaagctttc
     1981 ccgtctgccg gaataaaaac acaccggcct tttgcatcaa actcaagtcc gtggaaaggg
     2041 cactgtacac aatctccttt gaccttgcct ttgctgagcg ccgcgcctct gtgcgtacat
     2101 tgatcaacaa cacagcccag ttcaccctta ttatttctaa aaatggcaag ttccagattc
     2161 agtcttttaa tgccaacgat ctgatttgtt tttacaatcc tggaaggaag tatggcatac
     2221 cattgattcc gtatcatcag aaaccccttt ctccaatata tggtatctat tttctatcat
     2281 tttatgtttt tgccgcaacg atgtcaataa aataaaaaag ctttccgaaa cctgaccaca
     2341 gattccaaaa agctttcttt ttataatgta gatgatatat cttttaatat ttcagctgtt
     2401 gcacttaatt cttcaatgga cgccgccatt tcctccagcg atgcagcctg tgtctgcaca
     2461 atatcattcg tatcctttaa atcattggaa attttctcta ccatatgctc cacatcacta
     2521 agcacggaat caatctttgt gatcgattcg ttggtagagg tagataactt tcgaatctcc
     2581 tgtgcaacga tcgcaaatcc tcttccgttt tctcctactc ttgctgcctc gatcgctgca
     2641 tttaatccaa gcagatttgt ttcggaagcg atctctttta caaaggaaat cacctgatcg
     2701 gtatcttttg tcttttccgc tgcaatcttt gaagcctcaa aaatattatt attgtttttt
     2761 gcaagcgact gcacactgtc tgatattaca tttacggcat cggaaatctg ggtcagggag
     2821 cctgacagcg tttccgcagt atttaacact tcgtattttc tttttaggct ttttccaaca
     2881 acaaatacac ctattacctg atcgtcttct tttatcggaa ccgcatatga ctgaaatgca
     2941 ataccataaa ccgctttcgg aacttcctgt atgatcgttt tacctgtctt taaggcatta
     3001 tatacagcgc ctcccgaagg aacaggctgt ccttcctcaa tttttaaaac aagctctttt
     3061 ccctgctgaa ttttcaggta cttctccgta tcagcgatac caattgccac atcatcatta
     3121 aaaaaaatag g
//
LOCUS       DAOSYE010000181         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22725_length_3131_cov_3.168401, whole genome shotgun sequence.
ACCESSION   DAOSYE010000181 DAOSYE010000000
VERSION     DAOSYE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22725_length_3131_cov_3.168401"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..960
                     /locus_tag="PLU43_04160"
     CDS             70..960
                     /locus_tag="PLU43_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO27634.1"
                     /translation="MTVGIMQKYDTEAQEMTELTLCMGLLSTTPYYIVGLGINIYSIE
                     ELCFCLVQNAYILDRDLMDEKLCVFLEKQLKMTELSEKLKKLIDEEKSLGEFVTTILD
                     EVFYCDEEEIKHVKQVLVDNASLGFAQKRKARGDNLLKAKKYTLAIDEYQYVLQCIKK
                     EEEPELYAAILHNTGVAYAQLFLFEKAAGYYQEAVEMAGSRESLIQYLMAVRMTMRRE
                     QYERMLLRYGYEDAVVKEAEERMNTYRNAKPESSYAKSLEQIRELQSNGKVSAYYCRI
                     DDTLNIWKQEYRKNMIVRSR"
     gene            957..2189
                     /locus_tag="PLU43_04165"
     CDS             957..2189
                     /locus_tag="PLU43_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOO27635.1"
                     /translation="MMKKKWSEWGPIKFIKEKLGFVEEEDEFSLGLETGEAAEALTDV
                     LRHSPKRRKDLNILDYKERDLYIRECCEQMTVSSKEVESQKTEYRLVTERLSDLEEIA
                     SLPQSDINEVKKRVGKIIKIEEDEAAYRRPVSKITEAQYREMERMEDEIPDIISKLKK
                     DEDYQMTVRRDLNLLEGEKGALAYQRKEEKNRAANAKALSTIVVFAAVTAVCLLAVLQ
                     FALRLDVKMGCIVTAGLAAIALTAAFVSYRNAADNESKADRKLNRTISLQNTVKIKYV
                     NITNLLDYTYTKYKVNNSYELSYMWDKYLEEKEARHHTEEVAMKMEEARKSLFQLLVH
                     FHIKDPSIWLYQPNVLIYEEEMTEVRHSLIIQRQKLRKGIDFNLFNLEDYKNEIEQVV
                     KEYPQYAREILAIVSQYE"
     gene            complement(2173..2541)
                     /locus_tag="PLU43_04170"
     CDS             complement(2173..2541)
                     /locus_tag="PLU43_04170"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO27636.1"
                     /translation="MEEKRADRRLELGIKLSISDLYKENTNTIMDISSPIEVTDISAN
                     GIGFISECILPIGYYFIANLELTRDLPQIITDVRIIRSSAIDSTYYQYGCEFVSISPR
                     VKQMLTDFEKKLKEGSIHTD"
     gene            2783..>3131
                     /locus_tag="PLU43_04175"
     CDS             2783..>3131
                     /locus_tag="PLU43_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO27637.1"
                     /translation="MKKLELLYEGKAKKVFSTDDPDVLIVDYKDDATAFNGIKKGTIT
                     GKGVINNRMTNHVFKLLEKEGVPTHFIEELSDRETAVKKVEIVPLEVIIRNIAAGSFS
                     KRLGVEEGTPFKQP"
BASE COUNT          951 a          548 c          821 g          811 t
ORIGIN      
        1 ccttggtttc ggggaattat atccggcctc agggaaaata tgggacaagg aagttgagat
       61 cgtataatta tgactgtcgg gattatgcaa aaatatgata cagaggcaca ggaaatgaca
      121 gagctgacat tgtgtatggg tctattatct acaactccat attatatcgt agggcttgga
      181 atcaatattt attcgatcga agagctctgc ttctgtctgg tgcagaatgc atatatactg
      241 gaccgggacc tgatggatga gaagctctgc gtttttttgg agaagcagct gaaaatgaca
      301 gagctttccg aaaagctcaa gaagctgatc gacgaggaaa aaagtctcgg ggagtttgta
      361 actacgattc tggatgaggt tttttattgc gatgaggaag aaataaagca tgtgaagcag
      421 gtgcttgtgg ataatgccag tctgggcttt gcccagaagc gtaaggcgcg cggggacaat
      481 ctgctcaagg caaaaaaata tacacttgcg atcgatgagt atcagtatgt actccagtgt
      541 atcaaaaagg aagaagaacc ggagctttat gcggcgatcc tgcataatac gggtgttgcc
      601 tatgcacagc tttttctatt tgaaaaagcc gcaggctact atcaggaggc tgttgaaatg
      661 gccggcagca gggaaagcct gattcagtat ctgatggctg taagaatgac aatgcgcagg
      721 gaacagtatg agcgtatgct gctgcgttat gggtatgagg atgctgttgt aaaagaggcg
      781 gaagagcgga tgaatacata tcgcaatgca aagccggagt cttcttatgc aaagtcattg
      841 gagcagatcc gtgagctgca gagcaatggg aaggtcagcg cgtattattg ccgtattgac
      901 gatacgctga atatatggaa gcaggaatat aggaaaaata tgatcgtacg gagcagatga
      961 tgaagaaaaa atggtctgaa tggggtccga ttaagttcat aaaggaaaag cttggttttg
     1021 ttgaggaaga ggatgagttt tctctcggtc tggaaaccgg agaagccgca gaggcgctga
     1081 cggacgtact gcgtcattct ccgaaacgca gaaaagatct gaatattctg gattataagg
     1141 agagggatct ttatattcgg gaatgctgcg agcagatgac tgtttcttct aaggaagtgg
     1201 aatctcaaaa gacagaatac cggcttgtca cggaacgcct gtccgatctg gaggagattg
     1261 cgtcgcttcc gcagtcggat atcaatgaag tgaaaaagcg tgtggggaaa atcatcaaga
     1321 tcgaagaaga tgaggctgcc tacagacgtc cggtcagtaa gatcacggaa gcgcagtacc
     1381 gcgaaatgga acggatggaa gatgagattc cggatattat cagtaaactg aaaaaagatg
     1441 aggattatca gatgacagtg cgccgggacc tgaatcttct ggaaggggaa aagggagcac
     1501 ttgcctatca gcgcaaggaa gaaaagaaca gggcggcaaa cgcaaaggcc ctttcaacga
     1561 tcgttgtatt tgcggcggtg actgcagtct gtctgcttgc tgttctgcag tttgcgttaa
     1621 ggcttgatgt gaaaatgggc tgtatcgtga cggcgggact tgcggcaatt gccctgacag
     1681 cggcttttgt cagctaccgc aatgccgcgg ataatgaatc aaaagccgac agaaagctga
     1741 ataggacgat ctccctgcag aatacggtga agatcaaata tgtgaatatt acgaatctgc
     1801 tcgattatac ctatacgaaa tataaagtca ataattccta tgaattaagc tatatgtggg
     1861 ataaatatct ggaagaaaaa gaagcgcgcc accataccga agaggttgcc atgaaaatgg
     1921 aggaggcgag aaaaagcctt tttcagctgc tggtccattt tcatatcaag gatccgtcca
     1981 tatggctgta tcagccgaat gtgttgattt atgaggagga aatgacagaa gtacgccata
     2041 gtctgatcat tcagcgtcag aaactcagaa agggaattga ttttaatctt tttaatctgg
     2101 aggattataa aaacgagatc gaacaggtcg taaaggaata tccgcagtat gccagggaga
     2161 tccttgcgat cgttagtcag tatgaataga gccttctttc agtttctttt caaaatcggt
     2221 aagcatctgc tttacacgcg gggagatcga tacgaattcg cagccgtact ggtaataggt
     2281 gctgtctatt gcggaacttc tgataatccg gacatcagtg atgatctgcg gaagatcacg
     2341 cgtcagttcc agattcgcaa taaaataata gccgatcgga agaatgcatt ctgaaataaa
     2401 accaataccg ttggcagata tatcggtcac ttcgatcggt gatgagatat ccatgatcgt
     2461 attggtattt tctttgtata aatctgaaat ggataatttg atgccaagct ccaaacgcct
     2521 gtcggctctt ttttcttcca tgatgattct cccgtaaaat gatgtgtaca tagtttaact
     2581 tcttttgatt ataatacatt tgctgcaaaa aacaactgtt ttccgtaagt ttctgccgat
     2641 ttgtgattat tctcatattt tttatttttt tttgtacaga tagacggaaa tacggttgcc
     2701 atttgcgaaa cgatagtcta tactttgtgt tggactctga acaggtgatt ttattaaatt
     2761 tataataaag gagaaaataa ttatgaagaa attggagctg ctttatgagg gaaaggcaaa
     2821 aaaggttttt tccaccgacg atccggatgt gctgatcgtg gattataagg atgacgcgac
     2881 tgcctttaac ggaataaaga aaggtacgat cacaggaaaa ggcgtgatca ataaccgtat
     2941 gacgaaccat gtgttcaagc tgcttgagaa ggagggcgta ccgacacatt ttatcgagga
     3001 actgagcgac cgggagaccg cggtaaaaaa agtggagatc gttcctttgg aggtcattat
     3061 cagaaatatt gcagcaggca gtttttccaa aagactcgga gtggaagagg gaactccttt
     3121 taaacagccg a
//
LOCUS       DAOSYE010000182         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22739_length_3130_cov_3.274146, whole genome shotgun sequence.
ACCESSION   DAOSYE010000182 DAOSYE010000000
VERSION     DAOSYE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22739_length_3130_cov_3.274146"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="PLU43_04180"
     CDS             complement(<1..2045)
                     /locus_tag="PLU43_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO27638.1"
                     /translation="MNINKFTQKSIEAVNACEKLAYEFGNQEIEQEHLLYALLQQEDS
                     LILKLIEKMEIQKEHFINNAESWLNKRVKVQGGQVYIGQHLNKVLIHAEDEAKQMGDE
                     YVSVEHLFLSLLKYPNEAMKAIFKEYGITRDRFLQALSTVRGNQRVTSDNPEATYDTL
                     GKYGQDLVEQARNQKLDPVIGRDNEIRNVVRILSRKTKNNPVLIGEPGVGKTAVVEGL
                     AQRIVRGDVPEGLKNKTVFALDMGALVAGAKYRGEFEERLKAVLEDVKKSEGQIILFI
                     DELHTIVGAGKTDGALDAGNMLKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQP
                     VTVDEPTVEDTISILRGLKERYENFHGVKITDAALVSAAVLSHRYISDRFLPDKAIDL
                     VDEACALIKTEQDSMPTELDELNRKATQMSIEAEALKKETDKLSRDRLDALQKELAEL
                     NDEKNTKMAQWENEKASLVKLQNLRKEIEDVSNQIEILSREGDLSKVGELQYGRLPKL
                     KQQLAIEEDRVKAAGLTLVHENVSDEEIAKIISRWTGIPVAKLTESERSKTLHLDEEL
                     HKRVIGQNEGVTKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAQSLFD
                     DESNMVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTESVRRKPYSVVLFDEIEKA
                     "
     gene            complement(2253..>3130)
                     /locus_tag="PLU43_04185"
     CDS             complement(2253..>3130)
                     /locus_tag="PLU43_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOO27639.1"
                     /translation="PSHVKALYSKMSRQGYSRSTIKIIHDLIYPAFGMAVEDDIIRKN
                     PAKDTLRDYGEDPKEKEALTIEEQESFFKFVAESNIYNVYLPMLQVMVGTACRIGEII
                     GLTWDDVNMEDREVSIDHQLVYKDYKDGNGFCFHVHQPKTDAGIRVIPMTETVRKAFL
                     EQRKQLFTLGIPRDVEVDGLKGFIFIGRNGMPMMPSAVNNVLYNIVKAYNKKETETAK
                     KERRKPKLLPKFSAHILRHTGCTRMAERGIDPKVLQYLMGHANIAVTMEVYNHITSKK
                     RIENEIRKMDDLIAV"
BASE COUNT          686 a          851 c          584 g         1009 t
ORIGIN      
        1 gccttttcga tctcatcgaa caaaacaacc gaatacggct ttcttctgac tgattcagtc
       61 aattgtccgc cttcttcata tccgacatat ccgggaggcg ctccgatcag acgggatacg
      121 gagaatttct ccatatattc gctcatatcg atccgcacca tattgctttc atcgtcgaac
      181 aggctttggg caagcgcctt tgcaagttcc gtcttgccga caccggtcgg cccgagaaac
      241 aggaaagaac cgatcggttt tgtcggatct ttgatgccgg ctttggaacg gatgatcgct
      301 tccgtgactt tcgtcacgcc ttcattctga ccaatgaccc gtttatgcag ctcttcatcc
      361 aggtgcagcg ttttgctgcg ctcactctcc gtcagcttcg caaccggaat tcctgtccag
      421 cgggaaatga tctttgcgat ctcctcatcc gacacgttct catggacaag tgtcaggccg
      481 gctgccttca ccctgtcctc ttctattgcc agctgctgtt ttaatttggg cagcctgcca
      541 tactgcagct ccccgacctt gctcaggtca ccttcacggc ttaagatctc gatctgattg
      601 ctgacatctt caatctcctt ccgcagattc tgcaatttta caagagacgc cttttcattt
      661 tcccattgtg ccatttttgt gtttttctcg tcattcagtt ctgcaagttc tttctggagc
      721 gcatccagac gatccctgct taacttatcg gtctcttttt tcagcgcttc tgcttcgatc
      781 gacatctgcg ttgccttgcg gttcagttca tccagctctg tcggcatcga atcctgttcc
      841 gttttgatca gtgcacaggc ttcgtcgaca aggtcgatcg ctttatccgg cagaaatcgg
      901 tccgaaatat agcggtgaga caaaacagca gctgatacga gcgccgcatc agtgatcttc
      961 acaccgtgaa aattttcgta tctttccttc agcccccgca aaatggagat cgtatcctcc
     1021 actgtcggct catctaccgt aaccggctga aaacgccgtt caagcgcggc atccttttca
     1081 atatactgtc tgtattcatc cagcgtcgtc gcaccgatac agtgcagctc tccccttgca
     1141 agcatcggct tgagcatatt cccggcgtca agtgcgccgt cggtcttacc tgccccgacg
     1201 atcgtatgca gctcgtcaat aaacagaatg atctgtcctt cggatttttt aacatcctca
     1261 agcaccgctt ttaaccgttc ctcaaattca cctctgtatt tcgctcccgc tacaagagct
     1321 cccatatcaa gcgcaaacac tgttttgttt tttaagcctt ccggcacatc cccgcgtacg
     1381 atccgctggg ccagtccttc cacgaccgct gttttaccaa cgcccggttc cccgatcaga
     1441 acaggattgt tttttgtttt tctggacagg atccttacga catttctgat ctcattatct
     1501 ctgccgatca ccggatccag cttctggttt ctcgcctgct ccaccagatc ctgcccatat
     1561 ttcccaagcg tatcgtaagt ggcctccgga ttgtcgcttg tcaccctctg gtttccgcgc
     1621 actgttgaca gcgcctgcag aaaccgatcc ctcgtaatgc cgtattcttt aaagatcgct
     1681 ttcattgctt catttggata tttgagcagc gacagaaaca gatgctctac cgaaacatat
     1741 tcgtctccca tctgctttgc ttcatcttca gcatggatca gtaccttatt cagatgctga
     1801 ccgatataga cctggccgcc ctgtaccttt actctcttat tcagccagct ctccgcatta
     1861 ttaataaaat gttctttctg tatttccatt ttctcgatca gtttcagaat cagactgtct
     1921 tcctgctgta acagcgcata gagcagatgc tcctgctcta tttcctgatt tccgaattca
     1981 tatgccagtt tttcacaggc attgaccgct tctatggatt tctgtgtaaa tttatttata
     2041 ttcatggtcg tatctcctcc tcgttcaatc tgttatataa tatatagcac atgttgttag
     2101 cactgtcaac ggttgagtgc taatataatc tgttaatttt tgcaaagcct ttattttact
     2161 ggctttgcag gattctaaaa actttataaa atgtgatttt gggtgtttta cgccactttt
     2221 acgccaaatg cgtaagtgat tggcgtaaag atttacaccg ctatcaagtc atccatcttt
     2281 cttatttcat tttcaattct cttcttgctg gtaatatggt tgtacacttc catcgttact
     2341 gcgatattag cgtgtcccat gagatactgt agaacctttg gatctatgcc acgctctgcc
     2401 attctggtgc atccagtgtg ccttaggata tgagctgaga actttggaag cagttttggc
     2461 tttctccgtt ccttcttggc agtttctgtc tctttcttat tgtaggcttt tacaatatta
     2521 tagagaacat tgttcactgc actaggcatc attggcatac catttctgcc tataaatata
     2581 aatcctttta gcccatcaac ctccacatcc cttggtattc ccagtgtaaa caactgctta
     2641 cgctgttcta agaatgcttt tctgactgtc tctgtcattg gaattactcg aatccctgca
     2701 tccgtctttg gctgatgcac atgaaagcaa aaaccattgc catccttata gtctttatat
     2761 acaagctgat ggtcaatact aacctctctg tcttccatat tcacatcatc ccaggtcaga
     2821 cctataatct caccaattcg acatgccgtt ccaaccatta cctgtaacat cggaagatat
     2881 acattataga tgttactctc tgctacaaac ttgaagaacg attcctgctc ttcaatggtt
     2941 aacgcctctt tctcctttgg gtcttctcca taatccctaa gtgtatcctt tgcgggattc
     3001 tttcggataa tatcatcctc gacagccatt ccaaaagctg gataaatcaa atcgtgtata
     3061 atctttatcg tgcttcttga atacccctgt ctggacatct tggagtaaag tgctttcaca
     3121 tgagagggtc
//
LOCUS       DAOSYE010000183         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22756_length_3128_cov_4.216075, whole genome shotgun sequence.
ACCESSION   DAOSYE010000183 DAOSYE010000000
VERSION     DAOSYE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22756_length_3128_cov_4.216075"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PLU43_04190"
     CDS             <1..1496
                     /locus_tag="PLU43_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOO27640.1"
                     /translation="FVLKKALALKLPVIVCVNKIDRPEARCEDVVDEVLELFMDLDAD
                     DRQLDCPFIYTSAKTGQSGLDFKNIGPNMVPLFESVIDFIPAPQGDPESGTQVLISTI
                     DYNEYVGRIGVGKVDNGVIKVNQDAVIVNAHEPEKQKKVKVSALYEYDGLNKVNVREA
                     TIGSIVAISGITDIHIGDTICAVDNPEPIMFQKISEPTIAMNFMVNDSPLAGQEGKFV
                     TSRHLRERLFRELNTDVSLRVEETDTTECFKVSGRGELHLSVLIENMRREGYEFAVSK
                     AEVLYHTDSKGRKTEPMELAYIDVPDEFTGVVIEKLGQRKGELQNMSPITGGYTRLEF
                     SIPARGLIGYRGEFMTDTKGNGIINTVFDEYAPFKGDIQYRKQGSLIAFESGESITYG
                     LFNAQERGLLFIGPGEKVYSGMVIGQNGKPEDVEINVCKKKQLTNTRASGSDEALRLT
                     PPKILSLEQALEFIDTDELLEITPQSLRIRKKILDPTLRKRSSFKKG"
     gene            complement(1499..2830)
                     /gene="mgtE"
                     /locus_tag="PLU43_04195"
     CDS             complement(1499..2830)
                     /gene="mgtE"
                     /locus_tag="PLU43_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO27641.1"
                     /translation="MEEIQELIEKKQYTRLRQELAEWNEADIAAIFEELPGEELIKVF
                     RILPKTIAADVFAYLPIETEQEIITSLTDREAANIINNLMADDATDLMEEMPAGVVKK
                     LLANASSETRRDINHLLRYPEDSAGSVMTVEFVDLKETLTVQQAIEQIRRVGIDSETI
                     NICYVLDKERHLIGTVSLRYLLIRRADEMIGDFMNDNIIAVNTMTDQEEVAMLFSKYD
                     FTAMPVVDNENRLVGIITIDDVVDIIREETTEDIEKMAAIVPTDKPYMKMGIVETWKK
                     RIPWLLLLMISATVTGGIITHYEDALGTYVVLTSFIPMLMDTGGNAGGQASVTIIRGL
                     SLNEIEFKDMLRIIWKEARVGLFCGVTLAAANFVKILVIDRVTVPIAFVVCMTLVLAV
                     FIAKIIGCTLPMFAKKIGFDPAVMASPFITTIVDALSLMVYFQIATHFLHI"
BASE COUNT          870 a          781 c          683 g          794 t
ORIGIN      
        1 aattcgttct gaaaaaagcg cttgcactga agctgccggt catcgtatgc gtaaataaga
       61 tcgacaggcc tgaagcaaga tgcgaagacg tcgtagacga agtgctggag ctgttcatgg
      121 atctggatgc agacgacaga cagctggact gtccgttcat ctatacatcg gcaaaaaccg
      181 gtcagtcggg acttgacttt aaaaacatcg gtccgaacat ggtaccgctc ttcgaatcgg
      241 tcattgactt tattccggct ccccaggggg atcctgaaag cggtacgcag gtactgatca
      301 gtacaattga ctacaatgaa tacgtcggca ggatcggtgt cggcaaagtt gataacggcg
      361 tgatcaaagt caatcaggat gctgtcattg tcaatgcaca cgaacctgaa aaacagaaaa
      421 aagtcaaagt cagcgcactt tatgaatatg atggtctgaa caaggtcaac gtcagggaag
      481 ctacgatcgg ttcgatcgtg gccatttccg gtattaccga tatccatatc ggcgacacga
      541 tctgtgctgt ggacaatccg gaaccgatca tgttccaaaa aatttcagag ccgacgatcg
      601 ctatgaattt catggtcaac gattctccgc ttgccggaca ggaaggaaaa tttgtgactt
      661 cccgtcactt gcgggaacga ttgttccgcg aactcaacac agatgtatca ctgcgtgtgg
      721 aagaaaccga tacgaccgaa tgctttaaag tctccggccg cggtgaactg catctttccg
      781 ttctgatcga aaatatgcgg cgggaaggct acgagtttgc agtaagcaaa gccgaagtcc
      841 tctaccatac cgattcaaag ggcagaaaaa cagaaccaat ggaactcgcc tatatcgacg
      901 tacctgatga attcacagga gtcgttattg aaaagcttgg ccagaggaaa ggggaactgc
      961 agaatatgtc tccgatcacc ggaggctata caagactgga attttctatc cccgcacgcg
     1021 gtctgatcgg ctaccgcgga gaattcatga cagatacaaa aggaaacggc atcatcaata
     1081 ccgtatttga cgaatatgca ccttttaaag gtgacatcca atacagaaaa caaggttccc
     1141 tgatcgcgtt tgaaagcggt gaatccatca cctacggtct tttcaatgct caggaacgcg
     1201 ggcttctttt tatcggtccc ggcgaaaaag tatattccgg catggtcatc ggacaaaacg
     1261 gcaaaccgga agatgtcgag atcaatgtct gcaagaaaaa acagctgaca aatacacggg
     1321 cttccggttc ggacgaagca ctgcgcctta ctcctccgaa aatattaagt ctggagcagg
     1381 cgcttgaatt cattgatacc gacgagcttt tggagatcac gccgcaatcc ctgcgtatcc
     1441 gtaaaaaaat actggatccg acgctccgca aaagatcgtc ttttaaaaaa ggatgatcct
     1501 aaatatgtaa aaagtgtgtc gcgatctgaa aatagaccat caatgacagc gcgtccacga
     1561 tcgttgtaat aaacggactg gccatcactg ccggatcaaa cccaattttt ttggcaaaca
     1621 tcggcagtgt gcagccgata attttggcta taaatacggc aagaacaagc gtcatgcaga
     1681 caacaaacgc aatcggaacc gtcacgcggt caataaccag tatcttaaca aaattggccg
     1741 ccgcaagcgt aacgccgcaa aacagtccga ctcttgcttc tttccagata atacgcagca
     1801 tatctttaaa ctcgatctca ttcagagaca gaccgcggat aattgtaacc gacgcctgtc
     1861 cgccggcatt tccgcccgta tccatcagca ttggaataaa cgatgtcagc acgacatagg
     1921 tcccgagtgc atcttcataa tgcgtaatga tcccgccggt caccgttgcc gagatcatca
     1981 gcagtaaaag ccatgggata cgttttttcc atgtctccac gattcccatt ttcatatatg
     2041 gtttatcggt cggcacgata gccgccattt tttcaatatc ctctgttgtc tcttcccgga
     2101 taatatcgac aacgtcatcg atcgtaatga taccgacaag ccggttttca ttatcaacga
     2161 ccggcattgc agtaaaatca tattttgaaa aaagcattgc aacttcttcc tggtcggtca
     2221 ttgtatttac ggcaatgata ttgtcgttca taaagtcgcc gatcatttca tccgctctcc
     2281 ggatcagcag atatcttaac gacacggtcc cgatcagatg ccgttcctta tcaagcacat
     2341 agcagatatt gatcgtttcg ctgtctattc cgaccctgcg gatctgttcg atcgcctgct
     2401 gcacggtcag cgtttccttc agatcaacaa actcgaccgt cataacgctc cccgcggaat
     2461 cttccggata gcgcagcaga tgattgatat cccttcttgt ctcggagctt gcgttcgcaa
     2521 gcagcttttt tacgactccg gcaggcatct cttccatcag gtcggtcgcg tcgtccgcca
     2581 tcagattatt aataatattc gcagcttctc tgtctgtcag ggatgtgatg atctcctgct
     2641 ctgtttcgat aggaaggtac gcaaaaacat ctgccgcaat cgtttttggc agaattctaa
     2701 atactttgat cagttcctct cccggcagct cttcaaaaat ggcggcaata tcagcctcat
     2761 tccattctgc aagctcctga cgcaggcgag tatactgctt cttctcaatt aattcctgga
     2821 tttcttccat agacatagta gtattctcct ttgtatgcgg tttttatgaa tgtcatgcac
     2881 gttcgcccag gaacatctga tgacgagtta ttccctgtct tcttcacaaa aaccaccacc
     2941 tttctactat gtccggccgg tcttatcaga ccgctgccaa agacttgata cattcatatg
     3001 ttatgatacc gttacactat atcaaacggg cattttgaag tcaagaaaaa aataagtaaa
     3061 agcattaatc ttcttcaaac agcagcgtat catcgtttac ctgtgcataa gcacagccgg
     3121 cacccctg
//
LOCUS       DAOSYE010000184         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22817_length_3123_cov_3.589961, whole genome shotgun sequence.
ACCESSION   DAOSYE010000184 DAOSYE010000000
VERSION     DAOSYE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22817_length_3123_cov_3.589961"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLU43_04200"
     CDS             <1..630
                     /locus_tag="PLU43_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO27642.1"
                     /translation="TRNLSFQPLDLGLVTLGFDCAGARCLSTTNVDQVRTPEELKGVK
                     IRCMDAQVWQNVISALGATPVPIAYTELYTALQTNVVSGQDNPASNTVDGKFYEVLDT
                     FYETNHCYLCSGFYSNTTAWDALPDDYKSLLQGLLQKYLGNQYHDDITQFTEECYQTM
                     EDAGMKIVSQEDLDMDAFYSSASDMIDENYMNDESYAETINDVNETFGY"
     gene            660..1631
                     /locus_tag="PLU43_04205"
     CDS             660..1631
                     /locus_tag="PLU43_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOO27643.1"
                     /translation="MQKVLVSASHFDTLCKDAWKLMEANGIQVIFDPGRSFPAYSTEE
                     IERHPDREEIAAALIGMDDFRDEKKFKALPNLKAVAKFGVGVDNIDGALASQYGVKVL
                     NAPGQNSNAVAELTIAYILDLLRHVIILHKEMEQGKWPRFIGNEIKGKTVGLLGFGAI
                     AKLVARKLQSFDVEVLAYDLYPDQEAARRLGVKIVSQEEILTKSDIVSIHIPATADTY
                     HMFNDSTFASMKHGAYLVNPARGALVDLDALARAMQKGLIAGAALDAFEVEPLPKEAE
                     ILQCENIVLTPHTGAETEESYDHVSMTTAKDIISVLKGESPVNWVNK"
     gene            1650..2555
                     /locus_tag="PLU43_04210"
     CDS             1650..2555
                     /locus_tag="PLU43_04210"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOO27644.1"
                     /translation="MQKLYGVVIPLVTPLTEDDKIDVDSLKKLSEYCIEGGAQCLYPC
                     GTTGEMMYLTVAERKLVAETVVRQAAHRVPVFIQVGAWNLSDTKELAAHAVEIGADGI
                     GVVTPAFYGISDNGLVEYYVSVAKSVPEDFPVYLYGIKQNAVNDLNKEVCQGAADQCR
                     NIIGVKYSYPDMTRLQELMTVKNGEFDVLVGPDHLFEAVCAVGGKGVVSGNAMCIREH
                     YAALWKAIQDKDFDLATKIQRRTNILNETMCAVNNIAAYKVILKKEGVLQTTVMRKPM
                     ENLSSEQEKRLLEKMEAYHYKQVLI"
     gene            2589..>3123
                     /locus_tag="PLU43_04215"
     CDS             2589..>3123
                     /locus_tag="PLU43_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO27645.1"
                     /translation="MKVLNFGSMNYDYVYQVDHILTEGETSAAFSMERHLGGKGLNQS
                     IALAKAGIPVMHAGLIGEDGQAILDLCKKYGVDTSYVRQISGKCGHTILQVDSHAQNS
                     ILLYGGSNRMQTTAYMDEVLQSFQQGDYLLLQNEINHLDYLIDRAFDAGMKIILNPSP
                     FDEALYQCDLTKIDCFIL"
BASE COUNT          946 a          547 c          822 g          808 t
ORIGIN      
        1 actagaaatc tctctttcca accgcttgat ctcggccttg taacgcttgg atttgactgt
       61 gccggtgcga gatgtctgtc tacaaccaat gttgatcagg ttcggacgcc ggaagaactg
      121 aaaggtgtta aaattcgttg tatggatgct caggtttggc agaatgtaat ttctgcgctt
      181 ggggcaacgc ctgttccgat tgcctatacg gaattgtata cggcacttca gacaaatgtg
      241 gtttcagggc aggacaatcc ggcatctaat acagttgatg gaaaatttta tgaggtactg
      301 gatactttct atgaaacaaa tcactgttat ctgtgttcag gcttctatag taatacaacg
      361 gcctgggatg cacttccgga tgattataaa tcgctgctgc agggattatt gcaaaaatat
      421 cttggcaatc agtatcatga tgatattacg cagtttaccg aagaatgtta tcagacaatg
      481 gaagatgccg ggatgaagat cgtatcacag gaagatcttg atatggatgc attttatagt
      541 tctgcttctg acatgattga tgaaaactat atgaatgatg aaagttatgc agaaacaatc
      601 aatgatgtaa acgagacatt tggatattga taaatgatat aaaagaaaga agggaagaca
      661 tgcagaaagt gctggtatcc gcctctcact ttgatacatt gtgcaaagat gcctggaaac
      721 tgatggaggc aaatggaata caggtcatat tcgacccggg cagatcgttt cctgcatatt
      781 caacagagga aattgaacgg catccggaca gggaagagat tgcagctgcc ttgatcggta
      841 tggatgattt cagggatgag aagaaattta aagcactgcc gaatctgaaa gcagtcgcca
      901 aattcggagt cggagttgat aatattgatg gggcacttgc cagtcaatat ggtgtaaagg
      961 tgctgaatgc acccggacag aattcaaatg cggttgcaga acttaccatt gcctatatac
     1021 tggacctgct tcggcatgtg atcatactcc ataaagaaat ggaacagggg aaatggccgc
     1081 ggtttattgg aaatgaaata aagggaaaga cggtaggtct gctcggattt ggtgcaatcg
     1141 caaagttggt tgcaagaaaa ctgcagagct ttgatgtaga agtactggca tatgatctat
     1201 atcctgatca ggaagcggcc aggcggcttg gtgtcaaaat tgtatcacag gaagagatcc
     1261 tgacaaaaag tgatatcgta agtatccata ttccggcaac tgcagataca tatcatatgt
     1321 tcaatgacag tacatttgcg tctatgaagc atggcgcata tctggtcaat ccggcgcgtg
     1381 gagcgctggt tgacctggat gcactggcac gggccatgca gaagggactg attgccgggg
     1441 cagcattgga tgcatttgaa gtagagccgc ttccaaaaga agcagagatc ctgcagtgcg
     1501 aaaatattgt tctgacaccg cataccggtg ctgaaacgga agaatcctat gatcatgtca
     1561 gcatgacaac cgcaaaggat attatatctg tgctgaaggg cgaatccccg gtgaactggg
     1621 taaataagta aagacaaagg agaatgaaca tgcaaaagtt gtatggggtc gtgattccgc
     1681 tggtaacacc actgacagaa gacgataaaa tagatgtgga ttcattaaaa aagctttcag
     1741 agtactgtat tgaaggaggc gcacagtgct tatatccctg tgggacaaca ggggaaatga
     1801 tgtatttgac cgtagctgag agaaaactgg ttgctgaaac agttgtccgg caggcagcac
     1861 atagagtgcc ggtgtttata caagtaggtg cctggaatct ttctgataca aaagagctgg
     1921 cagcacatgc agttgaaatt ggagcggacg gaattggtgt ggtcacacct gctttttatg
     1981 gaatcagtga taatggactg gttgagtatt atgtgtcggt agcaaaaagt gttccggagg
     2041 atttcccggt atatctgtat ggaattaagc aaaatgcagt caatgacctg aataaagagg
     2101 tgtgtcaggg ggcggctgac caatgcagaa atattatcgg ggtaaagtac agctatccgg
     2161 atatgacaag attacaggaa ctgatgactg ttaagaatgg agagtttgat gtgcttgtcg
     2221 gacccgacca tttatttgaa gcagtctgcg ctgttggagg aaagggtgta gtttccggaa
     2281 atgcaatgtg tatcagagaa cattatgcgg cattgtggaa agcgatccag gataaagatt
     2341 ttgatctggc tactaaaatt cagcggcgta cgaacatttt gaatgaaacg atgtgtgcgg
     2401 taaataatat tgctgcgtat aaggtgattt taaagaaaga aggggttctg cagacaactg
     2461 ttatgcgaaa acccatggag aatctttctt ctgaacagga aaaaagattg ctggaaaaga
     2521 tggaagcgta tcattacaag caggttctga tttaacagac cgttgcctat atagagcagg
     2581 aggatatcat gaaagtattg aactttggat caatgaacta tgattacgtg tatcaggttg
     2641 atcatattct gacggagggt gagacatcgg cagcattttc catggaacgc catctgggag
     2701 ggaaagggct gaaccagtct attgcactgg ccaaggccgg aattcccgtt atgcacgcag
     2761 gactcatagg agaagatgga caggcgatac tggatctctg taaaaaatat ggagtggata
     2821 cctcttacgt cagacaaatc agcggaaagt gcgggcatac gatcttacag gtggacagcc
     2881 atgcgcagaa cagcatcctg ctttatgggg gcagtaaccg gatgcagaca acagcatata
     2941 tggatgaggt tctgcagtcg tttcagcagg gggactatct tttgcttcaa aatgaaatca
     3001 accacctgga ttatctgatc gatcgtgctt ttgatgctgg aatgaaaatt atcttgaacc
     3061 cttcaccatt tgatgaggca ttatatcaat gtgatctgac taaaattgat tgctttatac
     3121 tga
//
LOCUS       DAOSYE010000185         3118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22873_length_3118_cov_2.842638, whole genome shotgun sequence.
ACCESSION   DAOSYE010000185 DAOSYE010000000
VERSION     DAOSYE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22873_length_3118_cov_2.842638"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..562
                     /gene="lepB"
                     /locus_tag="PLU43_04220"
     CDS             20..562
                     /gene="lepB"
                     /locus_tag="PLU43_04220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO27646.1"
                     /translation="MTAKEEKKTSMRREIMEWIIVIEIAIILAVILNKFIIVNAVIPS
                     ASMETTIMTGDRIFGFRLSYLNEDPQRGDIVIFKFPDDEKQLFIKRIIGLPGETLEIR
                     DGKIYIDGSSEPLDEPYLNVVPTGDYGPITIPEGAYFMMGDNRNNSADSRFWNQPFVY
                     RNKILGKAVFRYFPNMGKIE"
     gene            603..1736
                     /locus_tag="PLU43_04225"
     CDS             603..1736
                     /locus_tag="PLU43_04225"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HOO27647.1"
                     /translation="MKNMIDYVNEYGKYPLKELPFNEVDSLILSQFAYLKYDGIVPSV
                     NENLPSVVLKDLPSHPEFSNLFGDERYEKVNRALFRAAADSSRFQNLRLNHYVNIVDT
                     GWEIQFSAVTYLLEDGLVYIAFRGTDETIVGWKEDFNMAFRTPVPAQEKAVQYLNYAA
                     AGIAGSFIVGGHSKGGNLAVYSAMKCRESVKERMIRIYSHDGPGFQQEVLDSGEFDVI
                     KDRIRKLVPHSSIIGMLLQTQETYEVVECKSFGVMQHDPFNWLVEGAGFKKAGGLYRH
                     VKMQDESVNQWISGMDKEQIREFVEQLFAVINAPGAATLIDLKADWKRSSSAMLGAIE
                     GMDEKSKEMMRLVMKNLFRAMSDVMKVRLTEMVQPATENEQSL"
     gene            1766..2203
                     /locus_tag="PLU43_04230"
     CDS             1766..2203
                     /locus_tag="PLU43_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOO27648.1"
                     /translation="MQFEKLQKDMIAAMKARDKERKDSISSMVSAVKKAAIDEGCRDD
                     IKEELVDRVLIKELKTAKEQIDTCPKEREDLLAQYQLRYDVIKEYAPQMMSEEEVEQI
                     IREKFADVLATKNKGAVMKAVMGELKGKADGKVINRIVGKLCG"
     gene            complement(2206..2973)
                     /locus_tag="PLU43_04235"
     CDS             complement(2206..2973)
                     /locus_tag="PLU43_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOO27649.1"
                     /translation="MHLEAKEHSLTGVKPSFGLSGSTLKIIAMVSMLIDHIGAAVLMP
                     YMEYILDTADYETIQKLTEIYEWMRRLGRFAFPIFCFLLVEGFFHTKNVWKYAGRLFL
                     FALISEVPFDLAFFNEPFRFAYQNVFFTLFFGLCTLIVLRAIENNERIKRYGRILLTF
                     AVIPAAMLITYFLKTDYDYKGILSIVILYLFCKTRIFQMVTGALSFSWEFPAPLSFIL
                     LWFYNGKRGWKMKYIFYIFYPLHLLILYLVRLPLCGS"
BASE COUNT          957 a          549 c          836 g          776 t
ORIGIN      
        1 aagacgaagg gaaaagtaaa tgacggcgaa agaggagaaa aaaacttcga tgagaagaga
       61 gatcatggag tggataattg taattgaaat tgcgattatt ttggctgtga ttttaaataa
      121 gtttattatc gtcaatgcgg tgattccgtc ggcctcaatg gaaacgacga tcatgacagg
      181 ggacaggatc tttggttttc gtctgtccta tctgaatgaa gatccgcagc gcggggatat
      241 cgtgatcttt aaatttccgg atgatgaaaa acagctgttt attaagcgta ttattggtct
      301 gccgggggag acgcttgaga tcagggacgg aaagatctac atagacggca gcagcgagcc
      361 gcttgacgaa ccgtatctga atgtcgttcc gaccggtgat tacggaccga taacgatacc
      421 ggagggggct tattttatga tgggtgacaa ccggaataat tcggcagatt cacgtttttg
      481 gaaccagccg tttgtctatc ggaataaaat acttggaaaa gcggtctttc ggtatttccc
      541 gaatatggga aagatcgaat agataaatgt aagaagccgt tgcattgtca gaggtggtct
      601 tgatgaaaaa tatgatagat tatgttaatg aatacgggaa gtatccattg aaggaactgc
      661 cttttaatga ggtggacagt ctgatattgt cacagtttgc ttatttgaaa tatgacggaa
      721 tagttccgtc cgtgaatgaa aatctgccgt cggtcgtttt aaaagacctg ccgtcccatc
      781 cggagttttc aaatttattt ggcgatgagc ggtatgaaaa agtaaacagg gcgctttttc
      841 gtgccgctgc ggacagcagc cgttttcaga atctgcggct gaatcactat gtgaatattg
      901 tcgataccgg ctgggagatc cagttttccg cagtgacgta tctgcttgag gatggacttg
      961 tatatattgc tttccgcgga acggatgaaa cgatcgtcgg ctggaaagaa gattttaata
     1021 tggcgtttcg cacgcctgtg ccggcacagg aaaaagctgt tcagtatttg aattatgcag
     1081 cggccggtat tgccggcagc tttattgtag gaggacattc caagggagga aaccttgcgg
     1141 tctattccgc gatgaaatgc cgtgaaagcg taaaagaacg gatgataaga atttatagtc
     1201 acgatgggcc cggatttcag caggaagtgc tggacagcgg tgagtttgat gtgataaagg
     1261 acaggatacg aaagctggtt ccgcattctt caattatcgg gatgctgctg cagacgcagg
     1321 agacctatga ggtcgttgaa tgtaaaagct ttggggtcat gcagcatgat ccgtttaact
     1381 ggcttgtgga aggcgcaggg ttcaaaaaag caggcggtct ttaccggcat gtcaaaatgc
     1441 aggatgaatc ggtcaaccag tggatcagcg gaatggacaa agagcagatc agggagtttg
     1501 tggaacagct ttttgccgtg atcaatgcgc cgggtgcggc gacgctgatc gatctgaaag
     1561 ctgactggaa gagaagctct tccgcgatgc ttggtgcgat cgagggtatg gatgaaaaat
     1621 caaaagaaat gatgcggctt gtgatgaaaa atctttttag agcaatgtcc gatgttatga
     1681 aggtcaggct gaccgaaatg gtacagccgg cgacagaaaa tgaacagtca ttataaaaag
     1741 acagatcaaa aaggaggtat ttgcaatgca gtttgagaag ttgcagaagg atatgatcgc
     1801 tgccatgaag gcgcgtgaca aggagaggaa ggacagtatt tcatcaatgg tttccgctgt
     1861 taagaaagca gcgatcgatg aaggctgcag ggatgatatt aaagaggaac ttgtagacag
     1921 agttctgata aaagagttaa agacggcaaa ggagcagatc gatacatgtc cgaaggagcg
     1981 cgaggacctg cttgcccaat atcagctgcg ttatgatgtt attaaggaat atgcgccgca
     2041 aatgatgtcg gaagaggaag tggaacagat cattcgggaa aagtttgcgg atgtgcttgc
     2101 cacgaagaac aagggagcag tcatgaaagc tgttatgggg gaactcaaag gcaaggcgga
     2161 cggcaaggtc atcaaccgga tcgtcggaaa actgtgcggc tgacattacg atccgcacag
     2221 cgggagtctg accagataga gaatcagtaa atgaagcgga tagaaaatgt aaaagatata
     2281 tttcattttc catccgcgtt ttccgttata gaaccagagc aggataaagg aaagcggcgc
     2341 gggaaattcc caggagaaag aaagagcgcc ggtaaccatc tgaaaaatac gggttttgca
     2401 gaacaggtac aggatcacaa tggaaagaat acctttgtaa tcataatcgg tttttaaaaa
     2461 ataagtgatc agcattgcgg ctggaatgac tgcaaaggta agcagaatcc tgccatatcg
     2521 tttgatccgt tcattatttt caatggcacg caggacgatc agagtacaca atccgaagaa
     2581 tagtgtgaaa aatacattct gatatgcaaa gcggaaaggt tcgttaaaaa aggccagatc
     2641 gaacggaact tctgaaatca gggcaaacag aaacagtctt cccgcatatt tccatacgtt
     2701 ttttgtatga aaaaaaccct caacaagaag aaaacagaaa attggaaaag caaagcgtcc
     2761 gagtctgcgc atccattcat aaatttccgt gagcttttga atagtttcat agtctgccgt
     2821 atccagaata tattccatat acggcatgag aacggccgca ccgatatggt cgatcagcat
     2881 tgaaaccatg gcaatgatct tcagggtgct tccggataaa ccaaaggaag gctttacgcc
     2941 ggtcagggaa tgttcttttg cttccaggtg catggcttgt ctccgtttct ttataatcag
     3001 ttaatcaata ttcaggccgc ttataaaacg atgattagta tagcatgatc tgttcaaatt
     3061 tacaagttag tgtaaaatta tagttggcaa aagcaataat gaatttatcc atgataag
//
LOCUS       DAOSYE010000186         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_22993_length_3106_cov_2.537856, whole genome shotgun sequence.
ACCESSION   DAOSYE010000186 DAOSYE010000000
VERSION     DAOSYE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22993_length_3106_cov_2.537856"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLU43_04240"
     CDS             <1..431
                     /locus_tag="PLU43_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27650.1"
                     /translation="LRKHVLDETTFYSEYGIATLAKDEKMFNLEATNNPSNWLGPVWL
                     VSNYIVFKGLLDYGYTKEAVLLCKNSLKLLGEDIKKTQCMHEYYDPYTGEPVMNGGFI
                     NWNILVLNMAEELRKEGIDYEGKNESLCADGEEGAPVDRT"
     gene            367..1413
                     /locus_tag="PLU43_04245"
     CDS             367..1413
                     /locus_tag="PLU43_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOO27651.1"
                     /translation="MKGKMKVCVLTGKKELQWIEREIPQPGRGELQIKLEYVGICGSD
                     LHFYQEGRLANWELNGPLALGHEPGGVVTAVGEGVTGFEIGDKVALEPGVGCGKCKEC
                     LEGHYNLCRNVKFMAIPNERDGVFSDYCVHDAVMCYKLPDTVGTMEGGLMEPLCVGLH
                     ATELSNARIGESAIVLGSGCIGLCTVMSLRARGVSEIYVSDVLEKRLEKALEVGATRT
                     FNSAKGEDIEAFAKTLPGGGVDQVYECAGNRFTTLQTCRLLRRAGKVTLVGVSPEPVL
                     ELDIATLNAMEGTIYSVYRYRNLYPKAIAAVASGMIPLKKIVSHTFAFKDCIHAIEYS
                     TEHKDEVIKAVVKF"
     gene            1457..3061
                     /locus_tag="PLU43_04250"
     CDS             1457..3061
                     /locus_tag="PLU43_04250"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOO27652.1"
                     /translation="MRIQNPIIPGFHPDPSIIRVNDTYYIANSTFEWFPGVSIHQSKN
                     LKDWELLTYPLATAQMLDLRGVLNGGGVWAPCLSYRDGTYYLVFSIVRTDEERMLDVD
                     NYLTTAGHIKGPWTMPVCLNSGGFDASLFHDEEDHTAWLVQMKWDFQPGNNLFRGIFM
                     QKYDDMHKKLVGKSICIFKGTDRGFTEGPHLYRHGAYYYLMTAEGGTRDGHCITMARS
                     LKREGPYETDPENPILSSAQQPAWPIQYAGHGDLFEDGHGNWYLVHLGVRRDLAAGYS
                     VMGRETFLQQCHWTKDHWFRPDTGRLPQETVEVRTVCEEHPIAETFYRTYTFDSPCLD
                     IHFSTLRIPLKEDCLSLKDRPGYLRLKGRESIFSKHHQVMAALRLSEISFQAETEFEF
                     EPENFQQTAGLTFFYNTANFYYCFISRETAVGKHLQVMLRDNNQMRYLLEDPIRLSEG
                     PIRIRAEFCPAGIQFFYSQGTDTTKAVINEKAEQLPIKILSDEYATESGEQGFTGTFL
                     GLCCQDLTGSRKPADFKYLKVSRLDP"
BASE COUNT          868 a          604 c          858 g          776 t
ORIGIN      
        1 agcttaggaa acatgttctg gacgaaacga ccttttactc ggaatatggg attgcgacac
       61 ttgcaaagga tgaaaaaatg ttcaatctgg aggcgaccaa taatccgtcc aactggctgg
      121 gtcctgtctg gcttgtttcc aattatattg ttttcaaagg cctgctcgat tatggatata
      181 caaaggaagc cgttctgctc tgtaagaatt ctttaaaatt attgggggaa gatattaaaa
      241 aaacacagtg tatgcatgaa tattatgatc catatacggg tgaacccgtc atgaacggcg
      301 gttttatcaa ttggaatata cttgttttaa atatggcaga ggaactgcga aaggaaggaa
      361 ttgattatga agggaaaaat gaaagtctgt gtgctgacgg ggaagaagga gctccagtgg
      421 atcgaacgtg agatcccgca gccgggcagg ggagagttac agatcaaact ggaatatgtc
      481 ggtatatgcg gttcggatct gcatttttat caggaaggcc gtcttgcaaa ctgggagctg
      541 aacggaccgc ttgcactggg acatgagccg ggcggtgtcg taacggcagt cggagaaggt
      601 gttaccggat ttgaaatcgg agacaaggtg gcactggagc cgggagtcgg ctgcggaaag
      661 tgtaaggaat gtctggaagg acattataat ctttgcagaa atgtaaagtt tatggcgatc
      721 ccaaatgaac gggacggtgt attttccgat tattgtgttc atgatgctgt aatgtgctat
      781 aagctgccgg atacggtcgg tacgatggaa ggcggtctga tggaaccgtt atgtgtcgga
      841 ctgcatgcga ccgagctttc aaatgccagg atcggggagt cggcgatcgt acttggaagc
      901 ggctgtatcg gactttgtac ggtcatgtca ctgcgggcac ggggtgtcag tgaaatttat
      961 gtttccgatg tattggaaaa aagactggaa aaggcactgg aagtcggtgc cacaagaacc
     1021 tttaacagcg caaagggaga agatatcgaa gcctttgcaa aaacacttcc cggaggcggg
     1081 gtcgatcagg tctatgaatg tgcgggcaac cgctttacaa cactgcagac atgccgtctg
     1141 ttaagaagag cgggcaaggt gacacttgtg ggagtttctc cggaaccggt gcttgagctg
     1201 gacattgcga cactcaatgc catggaagga acgatctatt ctgtctacag ataccggaat
     1261 ctttatccga aagcgatcgc tgccgtagct tccggcatga tcccgcttaa gaagatcgtt
     1321 tcccatacgt ttgcgtttaa agattgtata catgccattg aatacagtac ggagcataag
     1381 gacgaagtca taaaggcagt ggtcaaattt taaataccaa tatagaatct tacaggacag
     1441 atgaggtaca aaaacgatga gaattcaaaa tccgatcatt ccggggtttc atccggaccc
     1501 gtcgatcatt cgggtgaatg atacttatta tattgcaaat tcgacgtttg agtggttccc
     1561 gggtgtttcc attcatcagt caaagaatct gaaggactgg gaactgctca cttatccgct
     1621 tgccacggca caaatgctcg atctaagagg cgtattgaac gggggaggtg tatgggcacc
     1681 ctgtctaagc tatagagacg gaacctatta tcttgtattt tccattgtca gaacagatga
     1741 ggaacgcatg ctcgatgtcg ataactatct gacaacagcc ggtcatatca agggaccgtg
     1801 gacgatgccg gtctgtttaa acagcggagg atttgatgct tcgctgtttc atgatgaaga
     1861 ggatcatacg gcgtggctcg tacagatgaa atgggatttt caacccggaa ataatctttt
     1921 ccggggtatc tttatgcaaa aatatgatga tatgcataaa aaattagttg gaaaatcaat
     1981 ttgcattttc aaaggaaccg acaggggatt tacggaaggc ccgcatttgt accggcatgg
     2041 ggcatattat tacctgatga cggcagaagg cggaacaaga gacggacact gtattacgat
     2101 ggcaaggagc ctgaagcggg agggtccgta tgaaacggat ccggagaatc caatcctttc
     2161 ctctgcacag cagcctgcgt ggccgatcca atatgccgga cacggagatc tgtttgagga
     2221 tggacacggg aactggtatc tggtacatct gggtgtcagg agagatctgg cggccggata
     2281 cagcgtcatg ggacgggaga catttctcca gcaatgtcac tggacaaaag atcactggtt
     2341 ccggccggat acaggcagac tgccgcagga aacggtagaa gtgcggacgg tatgcgaaga
     2401 acatccaatt gcggaaacgt tttacagaac gtatacattt gactcccctt gtctggatat
     2461 ccatttttcc acgctgcgga tcccgcttaa agaagactgc ctgagtctga aagaccggcc
     2521 gggatatctg cgtctgaaag gacgggaaag tattttttca aagcatcatc aggttatggc
     2581 agctctcagg ctttcggaaa tttcatttca ggctgaaaca gaatttgaat ttgagccgga
     2641 aaattttcag cagacggcag gtctgacctt tttttataat acggctaatt tttattattg
     2701 ctttatcagt agggaaacag ctgtcgggaa gcatttacag gtgatgctcc gtgataacaa
     2761 tcagatgcgg tatctgcttg aagatccgat acggctgagc gaagggccga tccgtatccg
     2821 ggcagagttt tgtccggccg gtattcagtt tttttattcg cagggtacgg atacgacgaa
     2881 agccgtaata aatgaaaaag cagagcagct gccgattaag atcttatcgg atgaatatgc
     2941 aacagagagc ggggagcagg gatttaccgg cacctttttg ggattgtgct gccaggatct
     3001 gaccgggagc aggaaaccgg ccgattttaa gtatctgaag gtcagccgcc tggatccata
     3061 gttttgtacg gtattaataa aaacgggagg aattaagaat gataaa
//
LOCUS       DAOSYE010000187         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23047_length_3101_cov_3.061064, whole genome shotgun sequence.
ACCESSION   DAOSYE010000187 DAOSYE010000000
VERSION     DAOSYE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23047_length_3101_cov_3.061064"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLU43_04255"
     CDS             <1..208
                     /locus_tag="PLU43_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27653.1"
                     /translation="VIIIAHRLSTIREADQILVMQEGKIAERGRHGQLVSENGLYHRM
                     WEAHIDAGSWAVSKKSLQKEAGES"
     gene            205..1944
                     /locus_tag="PLU43_04260"
     CDS             205..1944
                     /locus_tag="PLU43_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27654.1"
                     /translation="MKKFFYNMSSGDPKTLFMPCFASFVNGLCKILPAALLFEVFNIV
                     YLYFSDTDVPMDLAHLWLVSAILIGWILVLFLAFSFEYNKTIAAAYKVSAEGRTALAE
                     HLRKLSLGFLESRDPGDLTTMMLGDYTQVENTNAHQLPQLVSAVTFPVLAFISLLFFS
                     WKMAFAMFAAIPVSLFIIWLSAGLQRRLGEKHVKAKVDSASRLQEYLICMREIKAYQM
                     GGKRFERLREAFYRLMKESIRMEGILGSVVMVAIAVLRSGLTVMVFTGAYLLIGGELT
                     LPVFLVFLLLGTRVFDPLTTVLTNYASLRYSVLSAERIMEIRHEKPLSGDGDVPEGNS
                     IRFEEVTFAYDQEDVLRNVSLQIPSNSITALVGPSGSGKSTITRLIARFWDVQGGRVL
                     LDGTDIKTIEPEKLLSRISMVFQDVYLFKDTIRSNILVGKPDAAQEEIERAAKQACCH
                     EFITKLPQGYDTPVGEGGCTLSGGEKQRISIARALLKDAPIVLLDEATASLDPENETQ
                     VQQAINELVKNKTVIMIAHRLKTIKGADHIIVLENGTVAEQGTHQELLIKEGLYARLW
                     KLQQQSAGWSMKV"
     gene            1941..2648
                     /locus_tag="PLU43_04265"
     CDS             1941..2648
                     /locus_tag="PLU43_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO27655.1"
                     /translation="MNEKDKSRTGLLDPRTKLFVVLFCGTFTFLADEKGLLLLFVMML
                     VYLSVQGMYKKALKFFPVFVLLFMLQHMITAYLTGAVAVLGFVVFIIVRFMPVFMAVS
                     VLSAVAPGELIAALQKLRLPKAVIIPLAVGLRFMPSISREFLAIRDAMCLRGISPFSI
                     NALRHPVMTMECIFVPLLMRSMKISDELAASASARGIDNPGSRTCLRQLYFGIFDGLA
                     LAVLLTAGIITGLFFIK"
     gene            2667..>3101
                     /locus_tag="PLU43_04270"
     CDS             2667..>3101
                     /locus_tag="PLU43_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27656.1"
                     /translation="MIKLKNVSFRYNSEQEQISEVSLSVKKGEFIILTGPSGCGKTTL
                     TRMINGLIPHFYEGRLTGELFLNGQNAEDLQPWNFGKLIGSVFQDPKSQFFAAVAEDE
                     IAFGCENYGVSAKEIKDRVDELSEALCIRPLLLRDLLTLSSGE"
BASE COUNT          781 a          626 c          848 g          846 t
ORIGIN      
        1 cgtgattatt atcgcacatc gtctttctac gatccgggag gccgatcaga tccttgttat
       61 gcaggaagga aaaattgcgg aacggggacg tcacgggcag ctggtaagcg aaaacggact
      121 gtatcacagg atgtgggagg cacatattga tgccgggtcc tgggcagtct caaaaaaatc
      181 tctccaaaag gaggcaggag aatcttgaaa aaattttttt ataatatgtc gagcggggat
      241 ccgaaaacat tgtttatgcc gtgttttgct tcttttgtaa acggattgtg caaaattctt
      301 ccggcagctc ttcttttcga agtgttcaat attgtttatt tgtatttttc agataccgac
      361 gtaccgatgg atctcgcaca tttatggctt gtaagcgcga ttctgatcgg ctggatactt
      421 gttttgtttc ttgcattttc atttgaatat aataaaacga tcgctgcagc ctataaagta
      481 tctgccgaag gaaggacggc actggcagag catttacgca agctgtcact gggatttctt
      541 gaaagccggg atccggggga tcttacaaca atgatgctgg gcgattatac gcaggtggag
      601 aatacgaatg cacatcagct cccgcagctt gtaagtgctg tgacatttcc tgtgctggca
      661 tttatttcac tgctgttttt cagctggaag atggcttttg ccatgtttgc ggccataccg
      721 gtctcacttt ttattatatg gctgtcggca ggactgcagc gccgtcttgg ggaaaaacac
      781 gtcaaggcaa aggtagacag tgccagtcgt ctgcaggaat atctgatctg tatgcgggag
      841 atcaaagcgt atcaaatggg aggtaaacgg tttgagcgcc tgcgcgaggc gttttaccgc
      901 ttgatgaagg aatcgatccg tatggaaggg attcttggtt ccgtcgtaat ggtcgcgatc
      961 gcagtgctgc gttccgggct gactgtcatg gtttttaccg gagcatatct gctgatcggc
     1021 ggtgagctta cgcttccggt ctttcttgtg tttttgctgc ttgggacccg tgtgtttgac
     1081 ccgctgacga ctgttcttac gaattatgcc tcgcttcgtt attcagtatt aagtgcggag
     1141 cggatcatgg aaattcgtca tgaaaagccg ctgtctggag acggggatgt accggaaggc
     1201 aacagtatcc ggtttgaaga agtgacgttt gcctatgatc aggaggatgt acttagaaac
     1261 gtttctcttc agattccgtc aaatagtatt acggcacttg tcggaccgtc gggaagcgga
     1321 aaaagcacaa tcacccgcct gatcgcccgg ttctgggatg ttcagggcgg cagggtgctc
     1381 ctggacggaa cagatatcaa aacgattgag cctgaaaagc tgttgtcccg tatttccatg
     1441 gtctttcagg atgtttatct ttttaaggat acgatacgca gcaatattct ggtcggaaag
     1501 ccggatgcag cgcaggagga gatcgagaga gctgcaaagc aggcatgctg tcatgagttt
     1561 ataacaaagc tgccgcaagg atatgatact cctgtcggag aaggaggatg tacgctttcc
     1621 ggaggagaaa agcagaggat atcgatagca cgggcactgt taaaggatgc gccgatcgtg
     1681 ctgctggacg aagcgaccgc atccctggat ccggaaaacg agacacaggt gcagcaggca
     1741 atcaatgagc ttgttaaaaa taaaaccgtt attatgatcg cacaccgtct gaaaacgatt
     1801 aaaggagcag atcatattat cgttttggag aatggaacgg ttgcagaaca gggaacgcat
     1861 caggaactgc ttataaaaga agggctgtat gcccgtctct ggaagctgca gcagcagtcg
     1921 gccggctgga gtatgaaagt atgaatgaaa aagataaaag caggacaggt cttcttgatc
     1981 cgagaacaaa actatttgtt gtgctttttt gcggaacatt tacatttctt gcagacgaaa
     2041 aaggattgct tctgcttttt gtcatgatgc ttgtttattt gtccgttcag gggatgtaca
     2101 aaaaggcatt gaaatttttt ccggtctttg tgctcctttt tatgctgcag catatgatca
     2161 cggcatatct gaccggagcc gttgcggtgc ttgggtttgt cgtgtttatt atcgtccggt
     2221 tcatgcctgt attcatggca gtatcggtgc tttctgccgt agcgccgggt gagctgatcg
     2281 ctgcgctgca aaagctgcgg ctgccaaaag ccgtgattat tccgctggca gtcggcctgc
     2341 gttttatgcc aagcatctcc agagagtttc tggcaattag agatgcaatg tgtctgcgcg
     2401 ggatttcgcc gttttcgata aatgcgctgc ggcacccggt tatgacaatg gaatgtattt
     2461 ttgtgccgct tctgatgcga agcatgaaaa tatctgatga actggcagcg tctgcttcgg
     2521 cgcgggggat cgacaatcca gggtcaagaa catgcttaag acagctgtat ttcgggattt
     2581 tcgacggatt ggccctggcc gtgcttttga cggcaggaat cataaccggt ctgtttttta
     2641 taaaatagat gaaggacgaa agagaaatga tcaaactgaa aaatgtcagt ttcagatata
     2701 acagtgagca ggaacagata tcagaagtat cgttgtccgt taaaaaagga gagtttatca
     2761 ttctgacagg gccttccgga tgcggaaaga caacacttac ccgtatgata aacggactca
     2821 ttcctcattt ttatgaagga aggctgaccg gagagctgtt ccttaatgga caaaatgcag
     2881 aggatcttca gccgtggaac ttcggaaagc tgatcggcag tgtatttcag gatccaaaaa
     2941 gtcagttttt tgccgctgtt gctgaggacg agattgcctt tggctgtgaa aattatggag
     3001 tatcggcaaa agagatcaaa gacagggtcg atgagttatc ggaagcgctc tgtatccggc
     3061 cgctgctttt aagggatctg ctcacgcttt ccagcggaga g
//
LOCUS       DAOSYE010000188         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23141_length_3093_cov_2.896972, whole genome shotgun sequence.
ACCESSION   DAOSYE010000188 DAOSYE010000000
VERSION     DAOSYE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23141_length_3093_cov_2.896972"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="gpmA"
                     /locus_tag="PLU43_04275"
     CDS             <1..702
                     /gene="gpmA"
                     /locus_tag="PLU43_04275"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOO27657.1"
                     /translation="QKNLFTGWTDVDLSKKGKEEAKTAGRLLKEEGYDFDLCYTSYLK
                     RAIHTLDRVLNEMDRVWLPVIKTWQLNERHYGALQGLNKAQTAEKYGDEQVKIWRRSF
                     DVAPPMLEEDDERNPKLQEQYRKEQGEVLPLGESLKDTIARVIPYYNEYILTDMKAGK
                     RVIIAAHGNSLRALVQYLEDLSKEEILGINIPTGVPLVYELSETGEFIDKKYLGDAAA
                     VQAKMNAVANQGKAK"
     gene            801..1418
                     /locus_tag="PLU43_04280"
     CDS             801..1418
                     /locus_tag="PLU43_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HOO27658.1"
                     /translation="MSRQVFELIRQMDIQDPEFQLAFQCAPLISGLKISNLLVIRPEK
                     LDEVKQILRNTDISYSVLSNTEEKAVILLYKQELLAAYLSMERVKKLLLRMGYRADTL
                     DTILPVFCIRYRKYVREKREFPHELGILLGYPIEDVEGFIDHNGRDFLFSGYWKVYEN
                     MPAKERLFQRFEHVKETMLQMIFCGMSIVDIIDIYSDRQIEKTAV"
     gene            1431..1862
                     /locus_tag="PLU43_04285"
     CDS             1431..1862
                     /locus_tag="PLU43_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOO27659.1"
                     /translation="MDKIYVVYWSQSGNTLAMANEIGEGIKAAGKEAVVETVSSVSTD
                     TLKDMKAFALGCPAMGAEVLEESEMEPFVAELEGTVSGKTIALFGSYGWGDGEWMRDW
                     AARMSSAGANVLNGEGLICHEMPDADGLAACRSLGEQLTAL"
     gene            complement(1900..2550)
                     /locus_tag="PLU43_04290"
     CDS             complement(1900..2550)
                     /locus_tag="PLU43_04290"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO27660.1"
                     /translation="MPKPRIIETNEGIQNELTAAVFDRFARGMRDKGWNGVEEMIASG
                     IKAGDLLEIGPGPGYVGLELAKKIVPNSLTGFEISPAMIKIAEKNAAEYGINAHYVQG
                     NAMEMPFDNESFDGVISNGSLHEWESPIRIFNEIHRVLRNGGTYCITDMRRDVCFIKS
                     KLIYLSTRPKEMRPGFLSSLNASYTLPEITMLLHDSALKNAIVKKDFFGLCIYGKK"
     gene            complement(2532..>3093)
                     /locus_tag="PLU43_04295"
     CDS             complement(2532..>3093)
                     /locus_tag="PLU43_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOO27661.1"
                     /translation="MVPLYILGLLLRFGPQHGYRIKKLIETELADFTQIKLPNIYYHL
                     EKMEAMDLIASNTEQNELHTGKKVYHVTKKGSAEFRSLLQNELHIGYRPVFESDALFY
                     FSDTLDQRELTDALEKYLAHLEKITAHIEEHKDTALSLLPEDAKSSADIIFKHHLLHY
                     RAETAWAKDALKTITDKENSQCQNQE"
BASE COUNT          855 a          588 c          815 g          835 t
ORIGIN      
        1 cagaaaaatc tttttacagg atggacagat gtcgatctgt cgaaaaaggg aaaagaagag
       61 gcaaagactg ccggccggct gctgaaagaa gagggctatg attttgatct ttgctatacg
      121 tcgtatctta aaagggcgat ccatacgtta gatcgtgttt tgaatgaaat ggaccgggta
      181 tggctgcctg ttatcaaaac gtggcagctg aatgaacggc attacggtgc gcttcaggga
      241 ttgaataaag cacagacggc tgaaaagtat ggagacgagc aggtgaagat ctggagacgc
      301 tcttttgatg tggcgccgcc gatgctggaa gaggacgatg aacggaatcc gaagctgcag
      361 gagcagtata gaaaagaaca gggagaagtg ctcccgcttg gggaaagcct taaggatacg
      421 atcgcaaggg tgattccata ttataatgag tacattttaa cagatatgaa agccggaaag
      481 cgtgtgataa ttgctgcaca cggaaattct ctaagggcgc ttgtacagta cctggaagat
      541 ctttcgaaag aagaaatact gggcatcaat attccgacag gagtaccgct tgtatatgag
      601 ctttctgaaa ccggtgaatt tatcgataag aagtatttgg gtgacgcagc ggccgttcag
      661 gcaaaaatga atgctgtggc aaatcagggt aaggcaaaat aaagaatatg agaaacacta
      721 tcacttgtcc gtcttgacta aatcatgatt tatagctatg ctagttctag ttagacaaaa
      781 ctaactggaa aggaggagct atgagtcggc aggtatttga acttatccgg cagatggata
      841 tacaggatcc ggagtttcag ctggcgttcc agtgcgcgcc gttgatttcc ggtttgaaga
      901 tatcaaatct gttagtgatc cggccggaaa aactggatga agtaaagcag atcttacgaa
      961 ataccgatat ttcttacagc gttctttcaa acacagagga aaaggcagtc atactgctgt
     1021 ataaacagga acttctggca gcgtatcttt ccatggaaag agtaaagaaa ctgcttctgc
     1081 gtatgggata ccgggcagat acgttggata ccatacttcc ggtcttctgc atccgttatc
     1141 ggaaatatgt acgggagaag agagaatttc ctcatgaact tgggattctc ctcggatatc
     1201 cgatcgaaga tgtagaggga tttatagacc ataacgggag agattttctt ttttcaggtt
     1261 attggaaggt atatgaaaac atgccggcaa aagaacgatt gtttcagcgg tttgaacatg
     1321 taaaagaaac aatgctgcaa atgatcttct gcggaatgtc tattgtggat attatcgata
     1381 tttacagtga cagacagatt gaaaaaactg cggtttagga ggaaatgaaa atggataaaa
     1441 tatacgttgt ttactggtca cagtccggaa atacactggc tatggcaaat gaaataggag
     1501 aagggatcaa agcagccgga aaagaggctg tggtggagac tgtaagcagt gtttctacag
     1561 atacactgaa ggatatgaag gcttttgcac ttggctgtcc ggcaatggga gcagaagtat
     1621 tggaagagtc ggaaatggag ccatttgtag cagaattgga aggaaccgtg tcgggaaaaa
     1681 ccattgcgct gtttggttcc tacggctggg gagacggtga gtggatgcga gactgggctg
     1741 cgagaatgag cagcgcaggc gcaaatgtat taaacgggga aggtctgatc tgtcatgaaa
     1801 tgcctgacgc ggacggactg gccgcatgca gaagtcttgg agaacagctt actgccttgt
     1861 aagaggccgg ttcgtccggt cggaatgatg tttgggaaat tattttttcc cgtaaatgca
     1921 gagaccgaaa aaatcctttt tgacgattgc atttttcaag gcggaatcat gcagaagcat
     1981 ggtgatttcc ggcagtgtgt aggaggcgtt cagggaagag agaaagcccg gacgcatttc
     2041 ctttggtctc gtggagaggt agatcagttt tgactttata aaacacacgt ctctgcgcat
     2101 atcggtgatg cagtaggtac cgccattacg caggacacgg tggatctcat taaaaatacg
     2161 gatgggagat tcccattcat gcagggaacc gttggaaatg acaccgtcaa aggactcgtt
     2221 atcaaagggc atttccatcg cgtttccctg tacataatgt gcattgatgc cgtattctgc
     2281 ggcatttttt tctgcgatct ttatcatggc cggactgatc tcaaagccgg tcaaagagtt
     2341 tggaacgatt ttttttgcca gctccagacc tacgtaaccg gggcccggtc cgatctccag
     2401 cagatctccg gcttttatac cggaagcgat catttcttcc acaccattcc atcctttatc
     2461 acgcatgccg cgtgcgaatc tgtcaaatac ggcagctgtc agttcatttt gaataccttc
     2521 gtttgtttcg attattcttg gttttggcat tgtgaattct ccttgtctgt tattgttttc
     2581 agagcgtctt ttgcccatgc ggtctcggcc cggtaatgga gcaggtgatg tttaaaaata
     2641 atatccgcag agcttttggc atcttcaggg agcaggctga gtgccgtgtc cttgtgttct
     2701 tcaatgtgtg cggtgatctt ttctaaatgc gccaggtact tttccaatgc gtctgtgagt
     2761 tcgcgctggt ccagtgtgtc actgaagtaa aataatgcgt ccgactcaaa gaccggccgg
     2821 tagccgatat gcagttcatt ctgcagcagg gaacgaaatt ctgcggatcc cttttttgtc
     2881 acatgatata cttttttccc ggtatgcagt tcattttgtt ctgtattgga agcaatcaga
     2941 tccatagctt ccattttttc aagatgataa taaatgttgg gcagttttat ctgcgtgaaa
     3001 tctgcgagct ctgtttcgat caatttttta atacgatacc cgtgctgcgg tccgaaacgc
     3061 agcaataggc ctaatatata gagcggtacc atg
//
LOCUS       DAOSYE010000189         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23163_length_3091_cov_4.100461, whole genome shotgun sequence.
ACCESSION   DAOSYE010000189 DAOSYE010000000
VERSION     DAOSYE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23163_length_3091_cov_4.100461"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLU43_04300"
     CDS             complement(<1..1203)
                     /locus_tag="PLU43_04300"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO27662.1"
                     /translation="MLVVKKFGGTSVANKERIMNVANRCIKDYRAGNDVVVVLSAMGK
                     QTDVLIDLAKDISPVPSKREMDMLLTTGEQISAALMAMAMDSLGVSAISLNAFQVAMH
                     TTSAYGNARLKRIDTERIRHELDQKRIVIVTGFQGVNKYDDFTTLGRGGSDTTAVALA
                     AALHADSCEIFTDVDGVYTADPRIVKNARKMKEITYDEMLDLATLGAGVLHNRSVELA
                     KKYGVQLVVRSSLTEAEGTVVKEDVKVERMLVSGVAADKNVARISMVGLKDRPGVAFR
                     MFDALAKANINVDMILQSIGRDNTKDITFTIPRDMADEALAALEAKKSVLGAQEIKCK
                     TDVAKVSIVGAGMMSNPGVAAKMFECLFNADININMISTSEIRVTVLIDENDVEKAMN
                     AAHEIFGLE"
     gene            complement(1262..2476)
                     /locus_tag="PLU43_04305"
     CDS             complement(1262..2476)
                     /locus_tag="PLU43_04305"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOO27663.1"
                     /translation="MKYMIVLGDGMADEPIAELGNKTPLAYAKTAALDQLSKLSEIGM
                     VKTIPDGMKPGSDTANLAVLGYDPKKYYSGRSPLEALSIGVPMKDTDIALRCNIVTIS
                     EEEPVFEEKRIIDHSSGEISTADAAVLLDAVREVLEDETYKFYVGTSYRHCLIWDKGS
                     VVDLTQPHDILTQKIGQYLPGDAHLKYMMKKSYEILRDHPINAERKRQGLNPANCCWF
                     WGAGTKPLLSSFEEKNHKRGVMISAVDLLKGIAIGAGMKVIQVEGANGGLHTNYEGKA
                     DAAVEALLHENYDFAYIHVEAPDEMGHQGSVERKVQSIEYLDERVIRRVREQMDASGE
                     PYRLLVMPDHPTPICIRTHTADPVPYLLYDSSAPLSNNWDYNEAAAEAAGNYVEKGYQ
                     LIDRLFENEPVV"
     gene            complement(2502..>3091)
                     /locus_tag="PLU43_04310"
     CDS             complement(2502..>3091)
                     /locus_tag="PLU43_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOO27664.1"
                     /translation="NHVDYEDIYTEGITKITAKDIAYVKKLGRAVKLLASSKRDGDNF
                     YAMVAPFMIPKSHPLYSVNGVFNAIFVNGNVLGDVMFYGSGAGKLPTASAVVSDVVDA
                     TKHLHRNIMSFWSSKKLELTSVENAARKFFVRIEGTPQARLEEVEAVFGMVEPVVLDR
                     ETEEFGFVTKEMSEKSYEDAAGRLSGVISRIRLEA"
BASE COUNT          743 a          873 c          559 g          916 t
ORIGIN      
        1 ctcaagaccg aatatttcat gtgcggcatt cattgccttt tccacatcgt tttcatcgat
       61 cagaaccgta acgcggatct ctgaagtaga gatcatattg atattaatat ccgcattaaa
      121 caaacattca aacatttttg ccgccacacc cggattagac atcatgcccg ctccgacgat
      181 cgaaaccttg gccacatcag ttttacactt tatttcctga gctcccagca cgcttttttt
      241 agcctccaat gctgcaagcg cttcatctgc catatccctt ggtattgtaa atgtaatatc
      301 tttcgtatta tcacgcccga tagactgtaa gatcatatcc acatttatgt ttgcctttgc
      361 cagcgcatca aacattctga acgcaacgcc cggtctgtct ttgagaccta ccattgaaat
      421 tcttgcaaca tttttatcgg cagcaacacc gcttactaac attctttcca cttttacatc
      481 ctccttaaca actgttcctt ctgcttctgt caagcttgac cgtactacaa gctgaacacc
      541 atattttttt gcaagctcca cagagcgatt atgcagaacg cctgccccga gcgttgccag
      601 atcaagcatt tcatcatatg tgatctcttt catcttacgg gcattcttta caatacgcgg
      661 gtcagccgta taaacaccgt caacatccgt aaagatctcg caggaatcgg catgcagcgc
      721 tgctgcaagt gcaactgccg tcgtatcgga accgccgcgg ccaagtgtcg taaaatcgtc
      781 atatttgttt acaccctgaa atccggttac gatcacgatc cgtttttgat cgagttcatg
      841 acggatccgt tccgtgtcga tccttttcag ccttgcattt ccatatgcgc ttgtcgtatg
      901 catggctacc tgaaaagcat tcagtgatat ggccgatacc ccaagggaat ccattgccat
      961 tgccattaat gcagcagata tctgttcccc cgtcgtaagc agcatatcca tttccctttt
     1021 agacggcacc ggactgatat cctttgccag atcgatcagc acatctgtct gttttcccat
     1081 cgccgacagt acaacaacca catcatttcc tgctctgtaa tccttgatgc agcggttggc
     1141 cacattcatg atcctttcct tattggccac tgatgtaccg ccgaatttct tcactactaa
     1201 catacaattc tcctcatttc ctgtttctat ttatttctta agtgattcta tttttacgct
     1261 cttatacgac cggctcattt tcaaaaagac gatcgatcag ctgataccct ttctccacat
     1321 aattgccggc agcttcagcc gcagcttcat tataatccca gttatttgaa agcggcgcac
     1381 tgctgtcata caataaatag ggaaccggat ctgccgtatg agtcctgatg cagatcggtg
     1441 tcggatgatc cggcattaca agcagtctgt acggttcgcc tgaggcatcc atctgctctc
     1501 tgacacgtct gatgacccgc tcatccagat attcgatcga ctgcactttt ctctctacgc
     1561 tgccctggtg tcccatttca tcgggcgcct ctacgtgaat gtaagcaaaa tcataatttt
     1621 catgcagcaa cgcctctacc gcagcgtctg cctttccttc ataattcgta tgaagtccgc
     1681 cgttggcgcc ttccacctgg atcactttca ttccggcacc gatcgcgatc ccctttaaca
     1741 gatcaaccgc ggagatcatc acacctcttt tatgattttt ttcttcaaag gaagaaagca
     1801 gcggttttgt tcccgcaccc caaaaccaac agcagttggc cggattcagc ccctgccgtt
     1861 tccgttctgc attgatcgga tgatcgcgta atatttcata actttttttc atcatatatt
     1921 tcagatgcgc atctcccgga agatactgtc cgatcttttg tgtcagaatg tcatgcggct
     1981 gtgtcagatc gacaacactt cctttatccc atatcagaca atgccgatag ctggttccaa
     2041 cataaaattt atatgtttca tcttcaagca cctctctgac tgcatccaaa aggacggcag
     2101 catccgctgt actgatctct ccggagctgt ggtcaatgat ccgcttttcc tcaaacacag
     2161 gttcttcttc tgaaatcgta acgatattac accgaagcgc aatatccgta tctttcatcg
     2221 gaacaccgat actaagcgcc tccagcggag aacggcccga ataatacttc tttggatcat
     2281 aaccaagcac tgcaagattt gccgtgtcgg aaccaggctt catcccatcg ggtatcgtct
     2341 tcaccatccc aatttcagac agctttgaca actgatcaag tgcagctgtt ttcgcataag
     2401 ccagcggtgt cttatttcca agctctgcga ttggctcatc cgccatacca tcgcctaata
     2461 ctatcatata tttcatttct ctaacctctt cctgataagc ctcaggcttc cagacggatc
     2521 ctgctgatca caccggacag ccggccggct gcatcttcgt atgatttttc gctcatttct
     2581 tttgtcacga aaccgaattc ttccgtttct ctgtcaagta cgaccggttc gaccattccg
     2641 aataccgctt ccacttcttc aagacgtgcc tgcggcgttc cctcgatgcg tacaaaaaac
     2701 tttcttgcag cattttcaac acttgtcagt tcaagctttt tactgctcca gaacgacatg
     2761 atatttctgt gcagatgctt tgttgcatcg actacatccg atacgaccgc acttgctgtc
     2821 ggcagcttgc ctgcaccgct tccatagaac atcacgtcac cgagtacatt gccgtttaca
     2881 aaaatcgcat tgaatacacc atttacactg taaagaggat gtgatttcgg aatcataaac
     2941 ggtgctacca tggcatagaa attatcgccg tcccgcttgc tggatgcaag cagcttcacc
     3001 gcccgaccaa gcttcttgac ataagcaata tcctttgccg taatctttgt gatcccttcc
     3061 gtataaatat cctcataatc cacatggttt c
//
LOCUS       DAOSYE010000190         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23272_length_3082_cov_3.172448, whole genome shotgun sequence.
ACCESSION   DAOSYE010000190 DAOSYE010000000
VERSION     DAOSYE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23272_length_3082_cov_3.172448"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLU43_04315"
     CDS             complement(<1..1145)
                     /locus_tag="PLU43_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27665.1"
                     /translation="MDNSKLKIVILDDNAERRNIISGFMPPYMECFAVPYGDTAKNLI
                     RPDADGRVTDMVIMNADDNKGHGLYMFDWMKTKEAGLGLKKIPVILLTAEEFSERVLD
                     FLEIGDAEFYVGEPDEDKLYSLIMRVFDKVELGPDPKEEPLLYTEERDADKISGLTVK
                     PVGEEGVLKRSIVLSLDEQRMHLEQALERGRKRTEQLRELIEMALRMKEEKKARLNME
                     QPESIQQKAPWTPVWIRPESQKYASGPVRLGASVYRNMSFIEPLNPDGADTKKTIVIV
                     DGDIRNLKTCELYLKNRYDVVSMETGMQAIDFFVRSKADLLLLSYAMPLLDGLKILES
                     IRWQPNGRMVPVIFMAEGNLLEIGKQCRQAGSAGLLRKPISKSALLGS"
     gene            complement(1164..>3082)
                     /locus_tag="PLU43_04320"
     CDS             complement(1164..>3082)
                     /locus_tag="PLU43_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO27666.1"
                     /translation="YDEKKLGSKISFKIGIYIFFSMVFIFLYCVAVYFYLRRTEAIRI
                     LEYYTSSNEIVLSADGPKLWLFVTVFFLWIISMLLTCVLIKRQIGKEIKPIGDAVSSM
                     EKSLHSAVDANESKNTFIANISHEIRTPMNAILGFSELILQTDSRDEINEYAQDIKRA
                     SNNLLAIINDLLDISKMESGRLELVQVPYYLHYLFTDVENVISIPIQNKGLKFRTNIS
                     PDLPGQLFGDIVRIRQVLINIINNAVKFTKEGYVEFSAYAMPVTDPEGEPVNDDRVVM
                     VFKIKDTGIGIRKEDQAAIFEKFRQVDAGSNKGIEGTGLGLSISDQLVRLMGGDISVS
                     SEYGKGTTFTVSICQKVLSRQKISEYAGNRETEDGNRQEKVFYAPGSRILLVDDNMVN
                     LRIMKGLLRHYQIEADTAESGYDALEMINKSAYDLIFVDYMMPGMDGIGTVHHIRRLK
                     DEEKKKTKVIAVSANAIRGIRDMFVAQGFDDYLSKPIVVYRLEKLLRTYLPKNLLVEG
                     IEIKSDLPSEADFKIEGIDICSGLKKCDNNVDDYLQILKIVYEYGENKCTELEQYIAA
                     GNYKDYVIAVHALKSVAVSIGAQELFSMARIYEMAGRNAQTEFLDENNKKLLTAYRML
                     IANIGAVLQEKKLL"
BASE COUNT          775 a          830 c          576 g          901 t
ORIGIN      
        1 gatccaagca gcgcgctttt ggatattggc ttccgcagca gtcctgcgga ccctgcctgc
       61 ctgcattgct tccctatttc aagaagattt ccctctgcca taaaaataac cggcaccatc
      121 ctgccgtttg gctgccagcg aatgctttcc agaattttta atccgtcaag caggggcatc
      181 gcataactta gtaacagcag atctgccttg gagcggacaa aaaaatcgat tgcctgcata
      241 ccggtctcca tactcacaac atcatagcgg tttttcagat acagttcaca ggtcttcaga
      301 ttacggatat ccccatcgac aataacgatc gtttttttcg tatccgcacc gtccggattc
      361 agaggctcaa taaaactcat attcctgtaa acagatgcac caagccgtac gggaccggat
      421 gcatatttct ggctttccgg ccggatccat accggcgtcc atggtgcctt ctgctgtata
      481 ctttcaggct gttccatatt cagccgggct tttttttctt ccttcatccg cagcgccatc
      541 tctatcagtt cccgcagctg ctccgtccgt tttctgcctc tctccagcgc ctgttccaga
      601 tgcatgcgct gttcgtccag actcaggacg atacttcgtt ttaagacgcc ttcctcaccg
      661 accggtttta cagtgagtcc gctgatctta tcggcatctc tctcctcggt atacagaagc
      721 ggttcctctt ttggatcagg tccaagctcg accttgtcaa atacccgcat gattaaagaa
      781 tacagcttgt cttcatcagg ctctccgaca taaaattccg catctccgat ctcgagaaaa
      841 tcaagaactc tttccgaaaa ctcttccgca gttaacagaa tcacaggaat ctttttgagt
      901 ccaagtccgg cttcctttgt cttcatccag tcaaacatat acagaccgtg tcctttatta
      961 tcgtccgcat tcataatgac catatcggtc actctcccgt ccgcatccgg acgtatcagg
     1021 ttttttgccg tatcgccata tggtaccgca aaacattcca tataaggcgg cataaatccg
     1081 cttattatat ttctgcgctc cgcgttatca tctaaaataa cgattttcag tttactgttg
     1141 tccataatat ccctttatct ccgttacaaa agcttctttt cctgaagcac cgcaccgata
     1201 ttggcaatca gcatacggta agccgtaagc agttttttat tattttcgtc aagaaactcc
     1261 gtttgtgcat ttctcccggc catttcataa attctggcca ttgaaaaaag ttcctgtgcc
     1321 ccgatgctca cggcgacact ctttaaggca tgcactgcga tcacataatc cttataattt
     1381 ccggccgcaa tatactgttc cagttccgta catttatttt caccatattc ataaacgatc
     1441 tttaagatct gcaaatagtc gtcaacgtta ttatcacact ttttcagccc gctgcatata
     1501 tcaattcctt cgatcttgaa atcagcctca gaaggcagat ccgattttat ctcaattccc
     1561 tcaacaagca gattcttcgg caaatatgta cgaagcagtt tctccagacg atagacaacg
     1621 atcggttttg aaagatagtc atcaaagccc tgcgctacga acatatcgcg gatgccgcgg
     1681 atcgcattgg ccgatacggc tatgaccttt gtctttttct tctcttcatc cttcagtctg
     1741 cggatatggt gtactgtccc gattccatcc attccaggca tcatatagtc tacaaagatc
     1801 agatcatacg cagatttatt gatcatctcc agcgcatcat atccgctctc cgccgtatcc
     1861 gcctctatct gataatgtct gagcagccct ttcataatac gcagattgac catgttgtca
     1921 tcaacgagca gaatcctgga acccggtgca taaaatacct tttcctgccg attaccatct
     1981 tctgtctccc ggttcccggc atattcggaa atcttctggc ggcttaacac cttctgacaa
     2041 atactgaccg taaatgtcgt accctttcca tattcactgg ataccgaaat atcaccgccc
     2101 atcagccgga caagctgatc ggaaatagaa aggccaagtc ccgtcccttc gattccttta
     2161 ttgcttccgg catccacctg cctgaacttt tcaaaaatag ccgcctgatc ctctttgcgg
     2221 atgccaatac cggtatcctt tattttgaag accattacga ctctgtcgtc atttaccggc
     2281 tctccttcag gatccgtgac cggcattgca tatgcggaaa actccacata tccttctttt
     2341 gtaaatttta cagcattatt gataatattg atcagtacct gtctgatccg gacaatatcc
     2401 ccgaacagct gtcccggcag atccggactg atattggttc gaaacttaag gcccttgttc
     2461 tgtattggga tggaaattac gttttccaca tccgtaaaca gatagtgcag ataatacgga
     2521 acctgtacaa gctccagcct ccctgattcc attttggaaa tgtccagaag atcgttgata
     2581 atggcaagca gattgtttga cgcgcgcttg atgtcctgtg catattcgtt aatttcatcc
     2641 cggctgtccg tctgtaagat cagttcggaa aaaccaagga tcgcattcat cggcgtcctg
     2701 atctcatgag aaatatttgc aataaatgta tttttggatt catttgcatc tactgcgctg
     2761 tgcagtgatt tttccatgga actgaccgca tcgccgatcg gcttgatctc cttgccgatc
     2821 tgcctcttga tcagcacgca tgtcagcagc atggaaatga tccacaggaa aaagacggtc
     2881 acaaaaagcc aaagcttcgg cccgtccgca gaaagtacga tttcgttgct ggacgtgtag
     2941 tattccaata tcctgatggc ttccgtccgg cgcaggtaaa aataaaccgc aacacagtat
     3001 aagaaaatga acaccatgga aaaaaaaata taaataccta ttttaaaaga tattttggag
     3061 ccaagtttct tttcgtcgta ca
//
LOCUS       DAOSYE010000191         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23277_length_3082_cov_2.819293, whole genome shotgun sequence.
ACCESSION   DAOSYE010000191 DAOSYE010000000
VERSION     DAOSYE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23277_length_3082_cov_2.819293"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLU43_04325"
     CDS             <1..296
                     /locus_tag="PLU43_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27667.1"
                     /translation="FSSAFSELIFDYIRLSVLERRNTLYGEKGIYQTVKNANEEWSFG
                     IDTGHVKSFLKTYHFNVVENLSSADLEDKYFKNGENGKINATHCIVHAIHAEV"
     gene            complement(293..952)
                     /locus_tag="PLU43_04330"
     CDS             complement(293..952)
                     /locus_tag="PLU43_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27668.1"
                     /translation="MRKFILEIVKIASFILFYKLVMSLFSNLLTLLLDLLLTVPAISG
                     LIAVYYDGLLYTLLPAACVITVCSLMMFLYKDGLHAISLILCLAFLSYLTADKIISAA
                     DSSGILSLDFAIQLWFSIILAGGFLYYLCNMTIAYYKKKSDTTDPDKNITIKNITTDQ
                     PLKEIKFNEKIATIILLILMVLIFGIIVPLIIEKNYEKKHGRQNTALPVQTITGNYAG
                     C"
     gene            1157..1537
                     /locus_tag="PLU43_04335"
     CDS             1157..1537
                     /locus_tag="PLU43_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27669.1"
                     /translation="MVSILVIDDSVFSQRVTADLIKKMLPEAEFHFANDGEQGLEAYK
                     ELNPDFVFLDLLMPKLNGLDVVRLLRKYDAGANIFVISADVQKSVKDKAVELGVMEFI
                     NKPFTAEKAELTAKIIRERINEAG"
     gene            1524..2180
                     /locus_tag="PLU43_04340"
     CDS             1524..2180
                     /locus_tag="PLU43_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOO27670.1"
                     /translation="MKQDEMYYDIITELFNISVGKAAGILSEIVNRKIILDVPEIKLI
                     LKEEDAVDLDACISDKISGALMVSSISFENNLKGKANLIFPTEKMKKFIHLCMNEEVA
                     GKDDFFSISEEEMDFTPIDFDVIMEIGNIVLNCIMGEIGNFLKVNFNYSLPDVKIFDR
                     QTFYNDSENDGYSHVLVLHITFVIDEIEIEGAIIVDLTLSSIQILLEKIKELEAEFHV
                     "
     gene            2173..3081
                     /locus_tag="PLU43_04345"
     CDS             2173..3081
                     /locus_tag="PLU43_04345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27671.1"
                     /translation="MYNLLNQVLDNVNEGIVILNKEFAVVYWNSYMERAAGIKKERAE
                     NQNIYELLPGLSRLCIDRCMEGVLKNGRPMFFSAAMHKRLFETEENYNFKISRLDSEI
                     PQILLEFVNITAQIMQIDQLRGAIGQLRKLNEELKEKEKIIKNMAYHDDLTGAASRTL
                     FYKRGNELLEKAKEQNGSLGLFFIDLDSFKQVNDVYGHIIGDWLLVQVAGVLKDAVRP
                     QDLVVRYGGDEFLILLPDVRSQDEYGEIMDRILAQQDRPFEYQDIKISISLSIGAAVY
                     PDDADTLDKLMAKADEKMYVKKCRKK"
BASE COUNT          985 a          492 c          724 g          881 t
ORIGIN      
        1 agttttcctc tgcctttagc gagctcatct ttgattatat acgtctgtct gtcctggaaa
       61 gaagaaatac gctgtatggt gaaaagggta tctatcaaac agtgaaaaat gcaaatgaag
      121 aatggagttt tggaatagat acgggacatg taaaatcgtt tttaaaaacg tatcatttta
      181 atgtagtaga aaatctcagt tccgctgatt tggaagataa atatttcaaa aatggggaga
      241 atggaaaaat aaacgctacc cactgtatcg tgcacgcaat acatgcagaa gtttaacagc
      301 cggcatagtt gccggttatc gtctgaaccg gcagggcagt attctgccgc ccatgttttt
      361 tttcgtaatt tttttctatg atcaacggta cgatgattcc gaaaataagc accattaaaa
      421 ttaatagaat gatagtcgca attttttcat tgaatttaat ttccttcaat ggctgatcag
      481 ttgtaatatt tttaatggtg atatttttat caggatcagt ggtatcgctt ttctttttat
      541 aataagcaat tgtcatattg caaagatagt ataaaaagcc gccggccagt ataattgaaa
      601 accatagctg gatggcaaaa tccaatgaga ggatgcctga actatctgcg gcagatatta
      661 ttttatcagc tgttaaatat gataaaaacg caagacaaag gatcagactg attgcatgca
      721 gaccatcttt gtaaagaaac atcataaggg aacatactgt aatgacgcac gcggcaggca
      781 gaagcgtata caacagaccg tcataataca ctgcaatcag gccgctgatc gcgggtacag
      841 taagcaataa atcaagaagg agtgtcagca ggttagaaaa taatgacatt acgagtttat
      901 agaagagaat gaaactcgca atttttacga tttccagaat aaactttctc atatcatgct
      961 ccctatgata tccgtatttg ctcttttgaa taacttcatg taagggtctg taaaattata
     1021 tcataaaaat gaaggttatg aaagcagaat cacgggatac aaagaagata cgaaaaatgt
     1081 gaagattctt gcaattccta tttgagtatg ctagaattac accatgtata aaacagctgt
     1141 gtatggagtg aagtttatgg ttagtatatt agtaattgat gattcggttt tttcgcagag
     1201 ggttacggca gatttaatta agaagatgct tccggaagcc gaatttcatt ttgccaatga
     1261 tggggaacag ggtttggaag cgtataaaga gctgaaccct gattttgttt ttttggatct
     1321 tctgatgcca aagcttaacg gacttgatgt ggtcaggctg ctgcgtaaat atgatgcagg
     1381 tgccaatatt tttgtcattt ccgctgatgt gcagaaaagc gtaaaagaca aagctgtgga
     1441 attaggtgtc atggagttta ttaacaaacc gtttacggca gagaaagcag agctgacagc
     1501 aaagatcatc agggagagaa taaatgaagc aggatgagat gtactatgat atcataacgg
     1561 agctttttaa tatcagcgta ggaaaagcgg cagggatcct ttccgagatc gtaaacagaa
     1621 agatcattct ggatgttcct gaaatcaaac tgatattgaa agaagaggat gccgttgatc
     1681 tggatgcgtg tatttccgat aaaataagcg gggcattaat ggtttcgtct atttcttttg
     1741 agaacaacct gaagggaaag gcaaatttga tttttccaac agaaaaaatg aaaaagttta
     1801 ttcatctttg catgaacgag gaagtggcgg gcaaagatga ttttttttcg atttctgaag
     1861 aggagatgga ttttacgccg atcgactttg acgtcattat ggagatcggg aatattgttc
     1921 tcaactgtat tatgggcgag atcggtaatt ttttaaaggt caattttaat tattcccttc
     1981 cggatgtcaa aatttttgat cgtcaaactt tttacaatga ctcggaaaac gacggatatt
     2041 cccatgtgct ggtgttgcat attacatttg tgatcgatga aatagaaatc gaaggtgcga
     2101 tcattgtgga tctcaccctc agttccatac agatattatt ggaaaaaatt aaagaattag
     2161 aggcagagtt tcatgtataa tttgctgaat caggttttag acaatgtaaa cgaaggaatt
     2221 gtcattttaa ataaagagtt tgcggtcgta tattggaatt cctacatgga gagagctgcg
     2281 ggaatcaaaa aagagagagc agaaaatcag aacatctatg aactgcttcc aggtttgtcg
     2341 cgcctttgta tcgaccggtg tatggagggg gtccttaaaa acggcagacc catgtttttt
     2401 tccgcagcca tgcacaagag actttttgaa acagaggaaa attataattt taaaatcagc
     2461 cgtctggaca gtgaaattcc gcagatcctt ttggaattcg tcaatattac ggcacagatc
     2521 atgcagatcg atcagctgcg gggagcgatc ggtcagctgc gaaagctgaa cgaggaattg
     2581 aaggaaaagg aaaagatcat taaaaatatg gcttaccatg atgatctgac aggggcagcc
     2641 agtcgtacgc ttttctataa aaggggaaat gaactgctgg aaaaggcaaa ggagcagaat
     2701 ggcagtcttg gtctgttttt tattgatctt gattctttta aacaggtcaa cgacgtatat
     2761 ggacacatta taggagactg gcttcttgtg caggtggccg gtgttttaaa ggacgcggtg
     2821 cgtccgcagg atctggtagt cagatacgga ggcgatgagt ttctcatact gctgccggat
     2881 gtccggtcgc aggatgaata cggtgagatc atggatcgca tcctggcaca gcaggacagg
     2941 ccgtttgaat atcaggatat taaaataagt atttcgctga gcattggggc ggcagtttat
     3001 ccggatgatg cagatacgct ggataagctc atggcaaagg ccgatgagaa aatgtatgta
     3061 aagaaatgcc ggaagaaata gc
//
LOCUS       DAOSYE010000192         3070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23413_length_3070_cov_2.998010, whole genome shotgun sequence.
ACCESSION   DAOSYE010000192 DAOSYE010000000
VERSION     DAOSYE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23413_length_3070_cov_2.998010"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="PLU43_04350"
     CDS             <1..1812
                     /locus_tag="PLU43_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27672.1"
                     /translation="IENSNKGVFMSAKKFITVYYPYILLGLGFLILLAKCFYSFCWSD
                     ETFYFSTAYRFYQGDSIFLHEWFPTQLSGVLLLPLLALYVTITGSTGGILLFFRICFV
                     CFALLSGIVTYRILKNTVSKTAALFCSLSVLFYAHLNIATLSYYTISIHCFLLSMLLI
                     YHFYQTGKKKMLVISGILFALCVLALPTMAIAYFLVAFCILLLLLVCRFIKLPDSFRQ
                     TVRRADLPAVLLYTLLGICIPAVIFFAFLLTNVSLTDFINAIPYVLSDEEHGTSLLYP
                     LKKFFIGINDVYGYAAYAGYLLILASFFLNRMLSPLFRRLFTIADLILFAVYFLYSFG
                     HTGYIQTALCMTALPLFFLAKKKNWKLFFLLFAGGMIFSVVYSYSSNGYLYILSMGHF
                     IASIAAILFIFEYAGEMIRGSAQNNRVFGRLAGFFCMAVICIALIQTIALRIVNIYRD
                     APLNKLTAKITAGPAAGLYTTPEHLSMYETVYNTLLTYCQSDSIGKKDGSIFITKLLP
                     YGYLCTDLKCAAPTTWRTAFDSKRLEPYYKMNPDRYPDLILVLDEAYGSYDTCGDVVA
                     DPNPNANEIGGFLLDYVTENNYSRLTVPCGTLYVRPD"
     gene            complement(1814..>3070)
                     /locus_tag="PLU43_04355"
     CDS             complement(1814..>3070)
                     /locus_tag="PLU43_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27673.1"
                     /translation="FDSWKLSCRQTRRKSFLPWIFSAAVLLLAVFAVSGLLSVSLSND
                     SMYYYSLYSKALVKFGTYRREFNVFLTDVGQGAALIGTLPYFFGFNETFGIQQFFNIN
                     FLCMFAYAVYEQALAAEGETAGPGRFIRRKRKAAVIAGLSVLVMLSSMPFVIVSKWVM
                     ANVYFMEYLFICGYLAYRFRPADQTVRENAAGMLLIEGLLIAVLSTLRMEGTIFVLLL
                     VLCISVLPYKSRELAFCFLLPAAMMQLLYDIRIFLTMQIAAPYRFLTGEKALVQLAAM
                     LAVLLYFLIVRGRFLKKLTEHMAAVIIAGLLLVNVGLCVLDWDIYIGNLEAFGANLFH
                     QSGWGLFPAFVLAMLFLLMKKERSFHYFDLLTVGYLLTTLAVCWARDGVLREGVGDSG
                     NRVLLQIIPLVVFALTRRTILWLKKD"
BASE COUNT          805 a          812 c          617 g          836 t
ORIGIN      
        1 atagaaaatt ccaataaagg agtctttatg tcggcaaaaa aatttataac cgtttattat
       61 ccatatatcc tgctgggact tggctttctc attctgcttg ccaaatgctt ttacagcttc
      121 tgctggtcgg acgaaacctt ttatttttcc accgcttacc gtttctacca gggagacagc
      181 atatttcttc acgaatggtt cccaacgcag ctgtccggtg tccttctgct tccgctcctt
      241 gcgttgtatg tgaccattac cggttccacc ggcggcattc ttttattttt ccggatctgc
      301 tttgtatgct ttgcgctgct ctcaggcatt gtcacctacc ggatcctgaa gaatactgtc
      361 tcaaagaccg ccgcgttatt ctgttcttta tcagtgctgt tttatgcaca tctaaatatt
      421 gcaacgcttt cctattatac gatttccatc cattgctttt tattgtccat gctgctcatt
      481 taccattttt atcaaaccgg gaagaaaaaa atgcttgtta tttccggcat tttgtttgcc
      541 ctttgtgtcc ttgcgcttcc aacaatggcg atcgcctatt ttcttgtggc cttttgtatt
      601 ctgctgctct tacttgtatg ccgttttata aaactgccgg actccttcag acagactgtg
      661 cggcgcgcag atctgcctgc tgttctttta tacacgctgc ttggcatctg tattcccgct
      721 gtcattttct ttgcttttct tctcacaaac gtgtcgctga cagattttat caacgccatt
      781 ccctatgtac tctcggatga ggaacacgga acgagcctgc tctacccgct gaaaaaattt
      841 tttatcggga tcaatgacgt atacggctat gcagcttatg ccggctatct gctgatcctg
      901 gcttcttttt tcttaaaccg catgctttcc ccgctgtttc gccgtctttt tacgattgcc
      961 gaccttatcc tgttcgccgt ctattttcta tacagcttcg gccataccgg ctatatacag
     1021 accgcactct gcatgacggc tcttccgctc tttttccttg caaagaaaaa aaactggaag
     1081 ctgtttttcc tgctgtttgc aggcggcatg atattttccg tcgtatacag ctattcttcc
     1141 aacgggtatc tctatatttt atccatggga cattttatcg cttcaatcgc agcaattctg
     1201 tttattttcg aatatgccgg cgaaatgata agaggatccg cccaaaacaa cagggttttc
     1261 ggacggcttg ccggtttttt ctgtatggca gttatctgta ttgccctcat ccagacgatt
     1321 gcgctgcgga tcgtcaatat ttacagggat gcgcctctta ataagctgac tgcaaagatc
     1381 accgccggac cggcagccgg tctttataca acaccggaac acctctccat gtatgagacc
     1441 gtttataaca cactgcttac ttattgtcaa tctgattcca tcggcaaaaa agacggcagc
     1501 atcttcatta caaagctgct gccctatggt tatctgtgta cggatctaaa atgcgccgct
     1561 ccgaccacat ggcggacggc ttttgacagc aagcgtctgg aaccctatta taaaatgaac
     1621 ccggaccgtt atccggatct catccttgta ctggatgaag catacggctc ttatgacacc
     1681 tgcggggacg tcgtcgccga cccgaacccg aacgcaaacg agatcggcgg gttcctgtta
     1741 gattatgtta ctgaaaataa ttacagcaga ctgaccgttc catgcggaac actctatgta
     1801 cggcctgact gatctaatct ttcttaagcc acaggatcgt cctccttgtc agggcaaaaa
     1861 caacaagcgg aatgatctga agcagcacgc ggtttccgga atccccgact ccttcacgca
     1921 agaccccatc ccttgcccag catacggcaa gtgtcgtcag cagatatccc actgtcagca
     1981 gatcaaaata atgaaaggaa cgctcttttt tcataagcag aaacagcatc gccagtacaa
     2041 aggccggaaa aagaccccat ccgctctgat gaaacagatt tgctccaaat gcttccagat
     2101 tgcctatata aatatcccag tcaagcacac ataacccaac attcacaagc agaagtcctg
     2161 caataatgac tgccgccata tgttccgtca gtttttttaa aaaccgtccg cggacgataa
     2221 gaaaatacag caggaccgca agcatggcag caagctgcac caatgctttt tcaccggtca
     2281 gaaaccgata aggcgcagca atctgcatcg tcagaaagat cctgatatca tataaaagct
     2341 gcatcatggc cgcaggcagc agaaaacaaa atgccagctc cctgctttta tagggaagta
     2401 cggaaataca aagtacgaga agcaggacaa agatcgttcc ttccatacga agagtcgaaa
     2461 gcacggcaat caaaagtccc tcgatcagga gcattcccgc cgcgttttcc cgcacagtct
     2521 gatccgccgg gcggaaccga tatgccagat acccgcagat aaataaatat tccataaaat
     2581 atacgtttgc catcacccac tttgacacga tcacaaacgg catgctgctg agcatgacga
     2641 gcaccgacag gccggcaata acggcagctt ttctttttct gcgtataaaa cgccccggtc
     2701 ccgcagtctc tccctctgcg gcaagtgcct gttcatagac cgcataggca aacatgcata
     2761 aaaaattaat attaaaaaac tgctggattc cgaacgtctc gttaaacccg aaaaaatacg
     2821 gtaaggtacc gatcagcgcc gcaccctgtc cgacatcagt caaaaataca ttgaattccc
     2881 gccgatacgt cccaaatttg acaagtgctt tggaatacag cgaataataa tacatggaat
     2941 cattggaaag actgacggaa agaagtccgg aaaccgcaaa aaccgcaagg agcagaacag
     3001 cggcagaaaa tatccacggc agaaaactct tcctgcgtgt ctgtctgcac gaaagcttcc
     3061 atgagtcaaa
//
LOCUS       DAOSYE010000193         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23547_length_3059_cov_3.021638, whole genome shotgun sequence.
ACCESSION   DAOSYE010000193 DAOSYE010000000
VERSION     DAOSYE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23547_length_3059_cov_3.021638"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLU43_04360"
     CDS             <1..983
                     /locus_tag="PLU43_04360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27674.1"
                     /translation="AFVALLETLTWLLAVPDSQTLKPFHYISNALFLSLDTLLAAFGL
                     RYLDYKIFVSKKKNRKNFFLYLAPVYLNIGFMIYNLFSDGFLFRVDAANQYHRGNATY
                     IGNISAYLVTIIVVLYFFSHRQMITGRITQTILCLTLLPIIGVIFQMLFYGLSAGIPA
                     YTLAIFISFLLLERDELLKDPLTQLDSRIQMEHRLQFKLKSQESFTAIIADVNDFKKI
                     NDIYGHTIGDKVLKDISGILLSHANLEDFVCRYGGDEFFIILESVDNIGHSYIQKIDQ
                     ALLAYSSHNPYPTTLSYGLLYVDHPVKFTMEELIRLTDQRMYEDKIRRKR"
     gene            complement(992..2131)
                     /locus_tag="PLU43_04365"
     CDS             complement(992..2131)
                     /locus_tag="PLU43_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27675.1"
                     /translation="MYSFDDCPDRRRTGAVKWDLAPEGVIPMWVADMDFAVAPPIEEA
                     LIRRVKHPLYGYPNEEAYGIKKLVAEHYRKLYHVEIEEAWIVWVPSVMPGLTMACKMQ
                     GGSFLYSVPMYNHIRMLDKETRLPVIEVDLKRNEENQYEMDFDAMEAAVTPACTTFIL
                     CNPHNPVGRMYTKPELEKVVDFCKRHHLLLASDEIHCELALNKAHIPAFAINKEAADI
                     SITLSSAGKICNIPGLPMGFAVIPNPEIRKRFLMQGDGMNACFHVLSAAAYEKAYDGS
                     CDAWKAELLAYLRNNQKLLEERLCSIPEITIPHNEGTYLSFFDCTLLGEEDPAAFFLR
                     EAKVMLSDGAEFGFSQGARLNFGCPKTQLNETLDRIERAVKLKRK"
     gene            complement(2133..>3059)
                     /locus_tag="PLU43_04370"
     CDS             complement(2133..>3059)
                     /locus_tag="PLU43_04370"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO27676.1"
                     /translation="LPAHRQGEFFHRYVLGLYRILGILTSEFPKILFESCASGGNRFD
                     LGMLCYMPQVWASDNTDAISRMSIQNGYSYGYPLSVIGAHVSGCPNHQTLRSTPLFTR
                     FHAACFGLLGYECNLLEMSKEEREAVRQQVALYKKWRQVFQFGDFYRLKDGSVIDSRN
                     PAGSYHWLCVSKDKKRAICMILQTKTISNTGYEKVRTRGLQEDLLYHFANVPGKIDIR
                     EFGDLINTISPIHIKKDSVMHELVSKFYRLQEEQEEYLVYGANLNRGGIKLKQAFSGT
                     GFNENTRHYTDYASRMYFMESEESIEMKKAGK"
BASE COUNT          785 a          765 c          582 g          927 t
ORIGIN      
        1 ctgcttttgt ggctctgttg gaaacgctca catggctgct ggctgttcct gacagccaaa
       61 ccttaaaacc atttcattat ataagcaatg ccctgtttct ttcccttgat actttacttg
      121 cagcattcgg acttcgctat ctggattata aaatatttgt ctctaaaaag aaaaacagaa
      181 aaaatttctt tctttatctg gcaccggtct atttaaatat cggatttatg atctataatt
      241 tattttccga cggttttctg tttcgtgtgg atgccgcaaa tcaataccac cgggggaatg
      301 ctacttatat cggaaatata tccgcctatc ttgttaccat cattgttgtc ctgtattttt
      361 tcagtcatcg gcagatgatc accggacgaa ttacacagac cattctctgt ctgacactgc
      421 tccccattat aggggtgata tttcaaatgc tgttttatgg attgtctgca ggcatcccgg
      481 cttatacgct agccatattt atcagttttc ttctgttaga gcgggacgaa ctgctaaagg
      541 atcccctgac tcagctggat tcacgtatcc agatggaaca ccggctgcaa ttcaagctga
      601 aatcccagga gtcattcact gccattatcg ccgatgtgaa tgatttcaaa aagatcaatg
      661 atatttatgg gcatacgatc ggcgacaaag tattaaagga catttccggc attcttctct
      721 ctcatgcaaa tcttgaggat tttgtctgcc gttatggcgg ggatgaattt tttattatcc
      781 tggaatccgt ggataatatc ggtcattcct atatacagaa aatcgatcag gctctgcttg
      841 cgtactcttc tcacaatcct tacccaacta cattaagcta tggtctttta tatgtggatc
      901 accctgtaaa atttacaatg gaagaattga tccggcttac cgaccagcgc atgtatgaag
      961 ataagatccg ccgaaaacgc taagacgacc tttattttct ttttaactta acagcccttt
     1021 ctatgcggtc cagcgtctca ttcagctgtg ttttcgggca tccgaaattc agtctcgcac
     1081 cttgtgaaaa tccaaattcc gcaccatcgg aaagcattac ctttgcttcc cgcaggaaaa
     1141 atgccgcagg atcttcttct cccagcagtg tacaatcaaa aaaggacaag taggttcctt
     1201 cattatgagg gatcgttatt tccgggatag aacaaagcct ttcttccaga agcttctgat
     1261 tattccgcag ataagccagc agttctgctt tccatgcatc acagctgcca tcatacgcct
     1321 tctcatacgc cgcagcagaa agcacatgaa aacaggcatt catgccgtcg ccctgcataa
     1381 ggaatctctt tcggatctca gggtttggaa tgactgcaaa tcccatcggc aagcctggta
     1441 tattgcatat tttccctgca ctggataatg tgatcgaaat atcggctgct tctttattga
     1501 tcgcgaaggc cggaatatgt gctttgttca gtgccagttc acagtggatc tcatccgaag
     1561 ccagcagcaa atgatgtctt ttgcaaaaat cgaccacttt ttccaattcc ggttttgtat
     1621 acatccggcc aacaggatta tgcggattac agaggataaa cgtcgtacac gcaggcgtaa
     1681 cggcagcttc catcgcatca aaatccattt catattgatt ctcttcattt ctcttcagat
     1741 caacttctat tacgggcagc cttgtctctt tatccagcat cctgatatga ttatacatcg
     1801 gaacagaata cagaaaagaa ccgccctgca tcttacaggc catcgtaaga cccggcatga
     1861 cgcttggcac ccatacgatc catgcttcct ctatttccac atgatagagc ttcctgtaat
     1921 gctctgccac caattttttt attccgtacg cttcttcgtt cggataccca tacaacggat
     1981 gttttactct tcttatcaac gcctcttcta tcggcggcgc aactgcaaaa tccatatcgg
     2041 cgacccacat cggaataacg ccttccggtg ccagatccca tttaacagca cctgttcttc
     2101 ttctgtcggg acaatcatca aaactgtaca tattacttcc ctgccttttt catttctatt
     2161 gattcttcgc tttccataaa atacattctg gatgcatagt ctgtatagtg ccttgtattt
     2221 tcattgaatc cggttccgct gaatgcctgc tttaatttga ttccgccgcg gttcaaatta
     2281 gcaccataaa ccaggtattc ttcctgttcc tcctgcagtc tgtaaaattt tgataccagc
     2341 tcatgcataa cagaatcctt cttaatatgg atcggtgaga tcgtattgat caggtctccg
     2401 aattccctga tatctatttt tccgggcaca tttgcaaaat gatacaacag atcctcctgc
     2461 agtccgcgtg tccttacttt ttcatatccc gtgttggata tcgtctttgt ctgcaggatc
     2521 atacagatcg ctcttttttt gtcttttgac acacataacc agtgataaga gcctgcagga
     2581 ttccggctgt ctattacaga accgtctttt aacctgtaaa aatctccgaa ttggaatacc
     2641 tgcctccatt tcttatagag agcgacctgc tgtctgaccg cttcacgttc ctctttgctc
     2701 atttccagca aattgcattc ataaccaagc aggccaaaac aggctgcatg aaatcttgta
     2761 aaaagcggtg tgctccgcag cgtctgatgg ttcggacagc cggatacatg tgcgccgatc
     2821 acggagagcg gatacccgta gctgtaccca ttctgaatac tcatcctgct gatggcatcg
     2881 gtattatcac ttgcccagac ctgaggcata taacacagca tgccaaggtc aaacctgttt
     2941 ccaccggaag cacagctctc aaaaaggatc tttggaaatt cggatgtaag aatacccagg
     3001 atccggtaaa gtcccaatac ataccgatgg aaaaactctc cctgtctgtg cgcgggcag
//
LOCUS       DAOSYE010000194         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23653_length_3049_cov_3.796927, whole genome shotgun sequence.
ACCESSION   DAOSYE010000194 DAOSYE010000000
VERSION     DAOSYE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23653_length_3049_cov_3.796927"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1150)
                     /locus_tag="PLU43_04375"
     CDS             complement(20..1150)
                     /locus_tag="PLU43_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOO27677.1"
                     /translation="MSKPAYLVLENGQIFKGKAFGAEKETIAEVVFTTGMTGYLETLT
                     DQSYFGQAVVQTFPLIGNYGIISPDSESASLSIGPKAYIVKHLCVHPSNFRSEENMDA
                     FFRKKGITGVYGIDTRALTRAIRSQGVMNGLITYDLAQQIDYEKIKNYTIENAVESVT
                     ISKPRVVEANSEHETIPTGIEKLKSPVRRKVALLDLGLKENIVRELSERNCEVTIFPS
                     FTSADRILEYEPDGIMLSNGPGDPTKNVDVIETVRTLCMSGVPIFGICLGHQIMALAN
                     GCLIRKMKYGHRGANQPVKNLETGRVSITTQNHGYVVKTGTIDEKIAREWYINANDGS
                     CEGIRYLDFPAVTVQFHPEACAGPQDTSYLFDNFMEMMGKEK"
     gene            complement(1214..2620)
                     /gene="purF"
                     /locus_tag="PLU43_04380"
     CDS             complement(1214..2620)
                     /gene="purF"
                     /locus_tag="PLU43_04380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO27678.1"
                     /translation="MSGKLHEECGVFGVSTHLDEAAGITYNGLLAMQHRGQEGAGIAV
                     IKGNAILYHKNTGLVNEVFSKDVMEKMPKSNLAVGHVRYSTTGSNTIHNVQPFITEFL
                     TGRLATVHNGNVINAKEIKHHLETCGLDFSATSDSEVISSLIAYKTVKEGSVEEGVVE
                     AAKKLKGAFSLIVLSGTHKIVAIRDANGFRPLCIGKNEYGMAVASESCALDSCGFRLI
                     RDIKPGEMVVIEDGKIIEERICLPKANEGICIFEYVYFARPDSVIDHLSVYEARHHMG
                     RILAREHPVDADIVCGVPDSGLEAAEGYAAESGIKLASGFVKNRYIGRSFIFPTQTQR
                     ENAVKLKLNPLGFNIAGKKIVVVDDSIVRGTTSAKLIQNLRNAGAREVHMRISSPPFK
                     HTCHFGTDIDREDNLIANRMSVEEIREQIGADTLGYISIGGLKEACKKCNMNFCTGCF
                     TGDYNIEVGDYLKSDLES"
BASE COUNT          745 a          806 c          536 g          962 t
ORIGIN      
        1 cgttattaaa cggcatctgt catttctcct ttcccatcat ttccatgaaa ttgtcaaaca
       61 aatatgaagt gtcctgagga ccggcacacg cttccggatg gaactgcacc gtaacagcag
      121 gaaagtccag atatctgatt ccctcacagc ttccgtcgtt cgcattgata taccattccc
      181 gcgcgatctt ttcatcgatc gtaccggtct ttaccacata gccatggttc tgcgttgtga
      241 tcgagactct cccggtctca aggttcttga ccggctgatt ggcacctctg tgcccgtatt
      301 tcatttttct gatcagacag ccgtttgcaa gcgccatgat ctgatgtccc agacagattc
      361 cgaaaatagg aactccgctc atgcagagcg ttcttacagt ttcaataaca tccacatttt
      421 ttgtcgggtc tcccggtccg ttactcagca taatgccgtc cggttcatac tccagtattc
      481 tgtcggcgct tgtaaaggac ggaaaaattg taacctcaca atttctctcc gacagttccc
      541 gcacaatatt ctccttcagg ccaagatcca gcagtgccac cttgcgtctg accggacttt
      601 tcagcttttc gataccggtt ggtatcgttt catgctcgct gtttgcctcg acaactcttg
      661 gcttgctgat cgtaacggat tctaccgcat tttctattgt ataatttttt attttttcat
      721 aatcgatctg ctgtgcaagg tcgtatgtga tcagaccatt catgacaccc tgacttctga
      781 tcgccctcgt cagcgccctc gtatcaattc catagacccc tgtaattcct ttttttctaa
      841 aaaaagcatc catattttct tcggaacgga agtttgatgg atgcacacat aaatgtttca
      901 caatataggc tttaggtccg atagaaagag aagcactttc ggaatccgga gaaattattc
      961 cgtaattccc gatcaatgga aatgtctgaa cgactgcctg tccaaaataa ctttgatcag
     1021 tcagtgtttc caaataccct gtcatgccgg ttgtaaatac gacttctgcg attgtctctt
     1081 tttcggctcc aaatgctttt cctttaaata tctgcccgtt ttcaagtact aagtatgccg
     1141 gtttgctcat cttgtagttc tcccttcttc aatccttttt gtaccggaat ccaaatctgt
     1201 tcctatttat atactagctt tccagatctg atttcaaata atcccctact tctatattat
     1261 agtcgcctgt aaaacatcct gtgcagaaat tcatattgca ctttttacat gcttccttca
     1321 atccgccgat actgatgtag cccagggtat ccgcgccgat ctgttctctg atttcctcaa
     1381 cactcatccg gttcgcgatc agattatcct ccctgtcgat atctgtgccg aaatgacagg
     1441 tatgcttaaa cggcggcgag gagatccgca tatgcacttc tcttgcccct gcattccgca
     1501 gattctgtat cagctttgcg cttgtcgtac cgcgcacaat ggaatcatcg actaccacga
     1561 tcttctttcc ggctatattg aatccaagcg gattcagctt cagcttcacc gcgttttccc
     1621 tctgcgtctg tgtcggaaaa ataaaacttc ttcctatata tctattcttg acaaatcccg
     1681 acgccagctt gataccgctc tctgccgcat acccttccgc tgcttccaga ccgctgtccg
     1741 gtacaccgca gacgatatcc gcatcgaccg ggtgctccct ggcaaggatc cttcccatat
     1801 gatgccgcgc ctcatatacg cttaaatgat caattaccga atcaggtctg gcaaaataaa
     1861 catattcaaa aatacaaatg ccttcattgg ccttcggcag acagatccgt tcctcaatga
     1921 tctttccgtc ttcgatcacg accatctctc caggctttat gtccctgata agccggaacc
     1981 cgcagctgtc aagtgcacag ctttccgacg caaccgccat tccatattcg tttttaccta
     2041 tacataaggg ccgaaaaccg tttgcatccc gaattgccac gatcttatgg gtacctgaca
     2101 gtacgatcag ggaaaacgca cccttcagct ttttcgctgc ctcgacgacg ccctcttcca
     2161 cactgccttc cttaactgtt ttgtatgcga tcagggaaga aatgacttca ctgtcactcg
     2221 ttgcggaaaa atcaagtccg cacgtttcca gatgatgctt gatctctttt gcattgatca
     2281 catttccatt gtggaccgtt gccagccgcc ctgtcagaaa ttccgtaata aacggctgta
     2341 cgttatgaat cgtattagaa cctgtcgtgg aatatcggac atgtccgact gccagattgc
     2401 tctttggcat tttttccata acatccttag aaaatacttc atttacaaga cccgtatttt
     2461 tatgataaag gatcgcattt ccttttatta ctgcaattcc ggcaccttcc tgtcccctgt
     2521 gctgcattgc aagcagtccg ttgtacgtaa tgcctgctgc ctcgtcaaga tgcgtactga
     2581 caccgaacac accacattcc tcatgcagtt ttccgctcat ctcgcaatcc tccttatgtt
     2641 gatattcctg cttacatcca aacagccgcc atgcttgcgc aaaacgaagt ctgataatac
     2701 aggcagacct tcttcctgtt ctttatcaga cccttctgtt tttaacctct aatctttcat
     2761 attttataaa gaaagacttt gcttgtcaat atgatttacc cctaaaaata aatttgcagc
     2821 agccaattat ttttccccgt ttttacagaa taactctcaa aaaacaaact tgcaatctgg
     2881 caaacagttt actttataaa ttttatctct gaaaatagat agggaataca ggatatcctg
     2941 tttcgttttc taaggttttt ttcaaaattt tcatattttt ttcatatttt ttttaagtta
     3001 gtgtaaaatt atagttggca aaagcaataa tgaatttatc catgataag
//
LOCUS       DAOSYE010000195         3029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23907_length_3029_cov_2.979153, whole genome shotgun sequence.
ACCESSION   DAOSYE010000195 DAOSYE010000000
VERSION     DAOSYE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23907_length_3029_cov_2.979153"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="hydA"
                     /locus_tag="PLU43_04385"
     CDS             complement(<1..1200)
                     /gene="hydA"
                     /locus_tag="PLU43_04385"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOO27679.1"
                     /translation="MKGQEVTEMEVFDRVIKNGTIITASDTYLADIGIRNGEIAAIGK
                     DLHADEVIDASGKYVLPGGIDVHTHLDMQLDDTLTSADDFETGSIAAAIGGTTSFIDY
                     ACQPKGGTLKDGLRIWKQKGEKACIDYGLHMAVVRADEETLLEMDDMMAEGVTSFKVY
                     LVYDMRVDDGELLKILEKSKECGGIVTVHCENYYVIRHRTEKLLAAHKTEPKYHPISR
                     PAACEAEAVNRVIKLAQLAGAPVYIVHNTCAEGVKRVKEAQEAGQPVLAETCPQYLFL
                     DDSAYDKPDFEGAKYVMSPPLRKQEDSAYLWERLWDGTLQVVATDHCPFSFEEKKRGL
                     HDFSKIPNGGTGIEERMLLMLSEGVKHHMTLNGIVRVTSLNPAKIFGMYPKKGTIAVG
                     SDADLVIY"
     gene            complement(1204..2424)
                     /locus_tag="PLU43_04390"
     CDS             complement(1204..2424)
                     /locus_tag="PLU43_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27680.1"
                     /translation="MKKKRLTSWERRQRRRIQIIIRAILLCVLCVAVIIAVKIFLPYE
                     TLNLSECFDVSYSGYNTKGSAVLTLDETKLDEVLTKVKSDYDTALFHTKECSDTDYDS
                     FAKSISAEVQDAENLSNGSKYTVSYQYDESLAKKLNIKVLSKSSDVTVAGLTTPVVLT
                     MDDLFQDIEVEFTGTSPNITMEIVNNSTNSFIQSMVFNPEEYQEYYATGDVVEIRAYF
                     SEEECLKQHYAIDTPSEECIKEYTVEGTDSYVSSASELSEELIEEAITAGKSAFVNAN
                     EYGVRIFCEANLVPVYINKKATFEWLTPSLISAYFKSVKSEAAGQDGNNYNDLDLIYF
                     VRITQADGVTCDAEAVVRFSNIIIKSDGTYEYDFSDPKIISASHSNASIVKNVVDKYE
                     DTYEIEKLDYSEYY"
     gene            complement(2572..3024)
                     /locus_tag="PLU43_04395"
     CDS             complement(2572..3024)
                     /locus_tag="PLU43_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO27681.1"
                     /translation="MDHIDIQILNMLQQNARMSLKELAAKVFLSAPAVSARIDALVKN
                     GFITGFHAQVDPIALGYHIKAFISLEVEPTDKNEFYPYVKNYPNIVECNCVTGDYSML
                     LEVMFHSTVELDQFIGELQRFGRTRTLIVFSTPVEQRGIRLPDKNEQE"
BASE COUNT          802 a          774 c          537 g          916 t
ORIGIN      
        1 ataaattaca agatctgcat cagagcctac ggcgatcgta cctttctttg ggtacatgcc
       61 aaagatctta gccggattga gcgaagtcac ccggacaatt ccattaagtg tcatatgatg
      121 tttcacacct tcggacaaca tcaggagcat acgttcctct attcctgtcc cgccgttcgg
      181 tattttagaa aaatcatgaa gaccccgttt tttttcttca aagctgaacg gacaatgatc
      241 ggtcgcaact acctgcagtg ttccgtccca cagacgttcc cacagataag ccgaatcctc
      301 ctgtttgcgg agcggtggag acatcacata ttttgcgcct tcaaaatccg gcttgtcata
      361 tgcactgtca tccaggaaaa gatactgagg acaagtttcc gcaagtacag gctgtccggc
      421 ttcctgggct tcctttacac gttttacgcc ttcggcgcag gtattatgaa caatatagac
      481 aggggcgccg gcaagctgtg caagcttgat cacccggttg accgcttcgg cctcacaggc
      541 cgcaggtctt gatatcggat gatatttcgg ttctgtttta tgcgctgcca aaagcttttc
      601 agtccggtgc ctgatcacgt aatagttctc acaatgcact gttacgatac caccgcattc
      661 ttttgacttt tccagtattt ttaaaagctc tccatcatcg acccgcatat catatacaag
      721 atacacttta aagctggtca cgccttctgc catcatatcg tccatttcca aaagcgtctc
      781 ttcatctgcc cgtacaactg ccatatgcag accataatca atgcaggcct tttcaccttt
      841 ctgtttccat atacgcaggc cgtcttttaa tgttccgcct tttggctgac aggcatagtc
      901 tataaaagag gttgttcctc caattgctgc agcaatggaa ccggtctcga aatcatctgc
      961 cgacgtcagt gtatcatcca gctgcatatc cagatgcgta tgtacatcga tcccccccgg
     1021 aagcacatat tttcccgaag catcgatcac ctcatccgca tgcagatctt tcccaattgc
     1081 cgcaatttcc ccgttcctga taccaatatc agccagatac gtatccgatg cggtaatgat
     1141 cgttccgttt tttattaccc ggtcaaatac ttccatctct gtcacctcct gccctttcat
     1201 actttaataa tattcactgt aatccagttt ctcgatctcg taggtgtctt catatttatc
     1261 aacaacattt ttcacaatgc ttgcattgct gtgggatgct gaaatgatct tcggatctga
     1321 aaaatcatac tcatacgttc catccgattt tataataata ttgctgaaac gcactacagc
     1381 ctccgcatcg caggtcacac cgtccgcctg cgtgatcctg acaaaataaa tcagatccag
     1441 atcgttatag ttattgccgt cctgtcctgc tgcttccgat ttcaccgatt taaaataagc
     1501 ggagatcagc gatggagtca gccattcaaa tgtcgccttc ttatttatgt aaaccggaac
     1561 gagatttgcc tcacagaata ttctgacacc atattcattg gcatttacaa acgccgactt
     1621 tccggccgtg atcgcttcct caataagctc ttcggaaagc tccgatgcag aagatacata
     1681 ggaatccgtt ccttccaccg tatattcttt tatgcattcc tcggaaggcg tatcaatggc
     1741 ataatgctgt ttcagacatt cttcttcgct gaaataggct ctgatctcca caacatcgcc
     1801 ggtcgcataa tactcctggt actcttcagg attaaaaacc atactctgaa taaaagaatt
     1861 tgtgctgtta ttcacaattt ccatcgtaat atttggtgaa gtaccggtaa actctacttc
     1921 aatatcctga aacagatcat ccatagtcaa tacaaccggt gtggtcagcc ctgctaccgt
     1981 aacatcgctg ctttttgaca gtactttaat gttcagtttt ttggcaagcg attcatcata
     2041 ttgataactg actgtatatt tactcccatt gctgagattt tccgcgtcct gtacttcagc
     2101 cgaaatactc ttggcaaaac tgtcataatc cgtatcactg cattctttcg tatgaaacag
     2161 tgccgtgtca taatcacttt tgacttttgt caatacttcg tccagctttg tttcatccag
     2221 cgtaagcaca gccgaaccct ttgtattgta acccgaatag ctgacatcaa aacactcact
     2281 taaattcagt gtctcatatg gaagaaaaat ttttactgca attatcacag ccacacatag
     2341 tacgcaaagt aaaatcgcac gaatgataat ttgtatccgc ctgcgctgtc tcctttccca
     2401 gctggtaagt cttttctttt tcataaaacc ctcgtaaaat ttttgttatg tctttttcat
     2461 gattccaatt caaaggataa tcatataaaa acacgcatat tattttagca caaaaaaacg
     2521 caaaaacaaa gagtgaaagc aaagttcact ctttgttcat aatttatcgc tttattcctg
     2581 ttcattttta tcgggaagtc tgattcccct ctgctccacc ggtgtggaaa acacgatcag
     2641 cgtacgagtt ctgccaaacc gctgcagctc tccgataaac tggtcaagct ctaccgtact
     2701 gtgaaacatg acttccaaaa gcatcgaata atctcctgtt acgcaattac attccacgat
     2761 gttcggataa ttttttacat aaggataaaa ttcattctta tctgttggct ccacttccag
     2821 gctgataaat gctttaatat gataaccaag cgcaatcgga tccacctgtg catgaaaccc
     2881 ggtaataaat ccgttcttta ccagtgcgtc gatcctcgca gaaaccgccg gagccgataa
     2941 aaatactttt gcggcaagtt cctttagcga catcctcgca ttttgctgca gcatgttcag
     3001 tatctggatg tcgatatgat ccaattcat
//
LOCUS       DAOSYE010000196         3024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23962_length_3024_cov_3.800269, whole genome shotgun sequence.
ACCESSION   DAOSYE010000196 DAOSYE010000000
VERSION     DAOSYE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23962_length_3024_cov_3.800269"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLU43_04400"
     CDS             complement(<1..409)
                     /locus_tag="PLU43_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOO27682.1"
                     /translation="MENNNKPGNNPNNKRNNPQNKQTLFILLIASLVMMLLLSYMRSF
                     LSSASTQKITYDEFIEKVENGEVESVKIGSDEITITPKESSNSSYMPITYYTVRVDDQ
                     ELPDRLINAGVKFEQEETNSSSMIVSVIVSYILP"
     gene            737..2341
                     /locus_tag="PLU43_04405"
     CDS             737..2341
                     /locus_tag="PLU43_04405"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOO27683.1"
                     /translation="MAVKYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPY
                     INIDPGTMNPIQHGEVFVTDDGTETDLDLGHYERFIDESLDKDSNVTTGKIYWSVLQK
                     ERRGDYGGGTVQVIPHITNEIKSRFYRNTTDDDMHIAIIEVGGTVGDIESQPFLEAIR
                     QFQHNVGRNNAILIHVTLIPYLRASQEMKTKPTQSSVKELQGMGIQPDIIVCRSEYPL
                     NKQLKDKIALFCNVPAYHVLQNLDVEYLYEAPLAMEKENLAQVACECLKIDCPEPDLV
                     DWQAMVDNMKSPTSEVTVALVGKYTQLHDAYISVVEALKHGGINQRAVVHIRWIDSST
                     VNAENADSVLAGLDGILVPGGFGDRGTDGMIQAINYARIHNIPFLGLCLGMQLFIVEF
                     ARNVVGYKDAHSIELDPATTHPVIALLPDQNGIEDIGGTLRLGSYPCVLNKNSKAYAL
                     YGQETIHERHRHRYEVNNEYRNDLISHGLLLSGISPDGRIVEMCELPDHPFFVATQGH
                     PELKSRPNRPHPLFKGFIEAALKANK"
     gene            2363..>3024
                     /locus_tag="PLU43_04410"
     CDS             2363..>3024
                     /locus_tag="PLU43_04410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO27684.1"
                     /translation="MVFNSYFFVLVFLPVSVIGYHMINKTNHFDIAKVFLLCMSLWFY
                     GCQNLHAAFVLLLSVFLNYAIVRFLFRDIFSRRLQKLFLAAGIVLNLSSLVYFKYLGF
                     FEVISNTVFHTKFTFLSLLLPLGISFFTFQQTAFLIDSYRNPGTRYRFLDYMLFVTFF
                     PKITVGPIALSTELIPQFNDSLRKKINFDNISKGIVSFSFGLAKKVLLADTLTKYVDW
                     GY"
BASE COUNT          823 a          653 c          645 g          903 t
ORIGIN      
        1 tcggaagtat gtaagaaaca atgacggata cgatcatcga agaggaattt gtctcctcct
       61 gttcgaattt cacgccggca ttaatcagtc tgtccggcag ctcctggtcg tctaccctga
      121 ccgtataata tgtgatcggc atataagagg aattcgaact ttccttcggc gtgatcgtga
      181 tctcatcgga accgatcttt acactttcca cttccccgtt ttctactttt tcaataaatt
      241 catcgtatgt gatcttctgg gtagacgcac tcgataaaaa gctcctcata taagaaagca
      301 gaagcatcat cacaagagaa gcaatcagaa gaataaacag cgtctgttta ttctgtggat
      361 tatttctttt attattgggg ttatttccgg gtttattatt gttttccatg gttttctcca
      421 ttttctatta gtcttatctc agcaattgta acgggaacgt ttttgaaatt cccgttattt
      481 atataaatga gattgttttc gctttctatt attatagaga ttgttcatag ataaatcaat
      541 atacacttta tgaaaaaaac tgccaatttc taaatcctgt ataaaatttc tatataaaaa
      601 atatactttt tttaaatctg ccactggatt tttatacaag aatctactat aataggaaag
      661 tctaaagaat aaactagcac ggtgcaaggg cgcgccgatt gtgtttcgcg ctctttggcg
      721 cagttgtgga ggacttatgg cagtaaaata cgtatttgtt acaggcggtg tcgtttccgg
      781 acttgggaag ggaattacgg ctgcatctct tggaaggtta ttaaaagcac gcggttataa
      841 ggttacgatg cagaaattcg atccttatat caatattgat cccggaacaa tgaatcctat
      901 tcagcatggc gaagttttcg ttacggacga cggcacggaa acagatctgg atcttggaca
      961 ttacgaacgg tttatcgacg aaagtcttga taaggactcc aacgtcacta ccggaaaaat
     1021 atactggtcc gtactgcaga aggaacggcg cggtgattat ggcggcggca ccgtccaggt
     1081 gatcccgcat attacaaatg agatcaaaag ccgtttttat cgcaatacta ccgacgatga
     1141 tatgcatatt gccattattg aagtcggcgg tacggtcgga gacatcgaaa gccagccgtt
     1201 tttggaagcc atccgtcagt tccagcacaa tgtcggccga aacaatgcca ttctgatcca
     1261 tgttactttg attccttatc tgcgcgcatc gcaggaaatg aaaacaaaac cgacccagtc
     1321 aagtgtcaag gaattacagg gaatgggtat tcagcccgat attatcgtct gcagaagcga
     1381 atacccgctc aacaagcagc tgaaagataa aattgccctg ttctgcaatg tgccggctta
     1441 tcatgtactg caaaatttgg acgtggaata tttatatgaa gcgccgcttg caatggaaaa
     1501 agaaaatctg gcacaggttg cctgtgaatg cttaaaaatt gactgcccgg aaccggatct
     1561 cgtagactgg caggcaatgg ttgataatat gaaatcccct acaagcgaag taacagttgc
     1621 gctcgtaggc aaatatacac agcttcatga tgcttatatc agcgtcgtgg aagcattaaa
     1681 acacggcggt atcaatcagc gtgctgttgt ccacatcaga tggatcgatt ccagtacggt
     1741 aaacgccgaa aatgcggatt ccgtgctcgc cggcctggat ggaattcttg ttccgggcgg
     1801 attcggagac cgcggtacgg acggtatgat ccaggcaatc aattatgcac ggattcataa
     1861 tattccgttc ctcggccttt gtctcggcat gcagcttttc attgtggaat ttgcaagaaa
     1921 cgtcgttgga tataaggacg cacacagtat tgaactcgat ccggcgacaa cacatcccgt
     1981 gatcgcgctg ctgcctgatc agaacggaat agaagatatt ggaggcacac tgcgtctcgg
     2041 ttcctatccg tgtgtactta ataaaaattc aaaggcttat gcgctttacg gacaggaaac
     2101 catccacgag cgccacaggc acaggtatga ggtcaacaac gaatatcgta atgatttgat
     2161 ttcacatggg ctgctgcttt ccggtatttc tccggacgga cgcattgtcg aaatgtgtga
     2221 gctgcccgat catccgtttt ttgtagccac acagggacat cccgaattaa aatccagacc
     2281 aaacaggccc catcctcttt tcaagggatt tatcgaagct gccttaaaag ccaataaatg
     2341 aaactgtatt taaggatacg ccatggtttt taattcttat ttctttgtac ttgttttttt
     2401 gccggtgtcc gtcatcggct atcatatgat caacaaaaca aaccactttg atattgcaaa
     2461 ggttttcttg ctgtgcatgt cattgtggtt ttatggctgc caaaacctgc atgcggcttt
     2521 tgttcttctg ctcagcgttt ttctgaatta cgcgatcgtc cggtttttat tcagggacat
     2581 tttttcaagg cgtctgcaaa agctttttct ggctgccggc attgtactga acctttcttc
     2641 attagtctat tttaaatatc tcggtttttt tgaagtaatc tccaacacgg tatttcatac
     2701 gaaatttaca ttcctcagcc tgcttcttcc gcttggcatc agttttttta catttcagca
     2761 gacagcattc ctgatcgaca gctatcgtaa tcccggcact cgataccgtt tccttgatta
     2821 catgctcttt gtgacgtttt tcccgaaaat tacggtcgga ccgatcgctc tttccacaga
     2881 attgattccg cagtttaatg attccctgcg caaaaaaata aattttgaca atatttcaaa
     2941 aggaatcgtt tctttttcct tcggacttgc caagaaagta ctgcttgccg acacactgac
     3001 aaaatatgtg gactggggat attt
//
LOCUS       DAOSYE010000197         3024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_23966_length_3024_cov_2.489390, whole genome shotgun sequence.
ACCESSION   DAOSYE010000197 DAOSYE010000000
VERSION     DAOSYE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23966_length_3024_cov_2.489390"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /gene="guaB"
                     /locus_tag="PLU43_04415"
     CDS             <1..1313
                     /gene="guaB"
                     /locus_tag="PLU43_04415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOO27685.1"
                     /translation="MDTVTEHRMAIAMARQGGIGIIHKNMSIEEQADEVDKVKRSENG
                     VITDPFSLSPEHTLKDANELMGKYRISGVPITVGRKLVGIITNRDLKFETDLSKKIKE
                     RMTAEGLITAMEGITIEDAKKILANARKEKLPIVDKDFNLKGLITIKDIEKQIKYPLS
                     AKDAQGRLLCGAGIGITANVLERVEALVKAKVDIVVLDSAHGHSENVLRCARMIKEAY
                     PKLQLIGGNVATSEGTKALIEAGVDAVKVGIGPGSICTTRIVSGIGVPQVTAIMDCYK
                     AAKEYGIPVIADGGIKYSGDMTKALAAGASVCMMGSIFAGCDESPGTFELYQGRKYKV
                     YRGMGSLSAMENGSKDRYFQADAKKLVPEGVEGRVAYKGTVEDTVFQLIGGIRSGMGY
                     CGTPTIEDLKQNGRFVKISAAALKESHPHDIHITKEAPNYSIDE"
     gene            1328..2107
                     /locus_tag="PLU43_04420"
     CDS             1328..2107
                     /locus_tag="PLU43_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27686.1"
                     /translation="MGSYIARELKKALKRRYTYAYVLGILILCLLANIAVMAFRTIYG
                     TNEGTYAYNLIEYATWCFVLPYYSCIFIADMGFGKIYPNPHIKDNVTKNLSRTQIYLS
                     KLITEVLLAIVFLVIAFILLISITALFQLRDRTICSYTIWDFTQKMVFATPLWIAGIS
                     FGNMFLFLFEDKRKAYVGFFILTFAVPRIIMQLAAEPLKLAPFKVIRTYLITQNFSLL
                     PYPADPARNIPLTVALGFIYAAIASVIGAIVYNKKKTDYIL"
     gene            2293..>3024
                     /locus_tag="PLU43_04425"
     CDS             2293..>3024
                     /locus_tag="PLU43_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOO27687.1"
                     /translation="MSLSDVKMIFKFVGGLGFFLYGMNQMADGLQKSAGSRMKNLLGY
                     LTNNKFIAILVGTLVTAIIQSSSATTVMVVGFVNAGIMDLTQAVGVIMGANIGTTVTA
                     WIVSLGSIGSALKPEFFAPLLVGIGAFVLLFSKSEKKRDAGEILVGFGALFVGLSFMS
                     GSIEPYRDSSLFSDAFRTLGHNPILGILVGLVVTSIIQSSSASVGILQTLAMNGMVTW
                     NSAVYITLGQNIGTCVTALISSIGAG"
BASE COUNT          797 a          586 c          811 g          830 t
ORIGIN      
        1 gcatggatac ggtgacagag caccgcatgg cgattgcaat ggccagacag ggcggtatcg
       61 gtattattca caaaaacatg tctattgaag aacaggcaga tgaagtcgat aaggtgaagc
      121 gttcagaaaa tggcgtcatt accgatcctt tttctctttc accggaacat acgctgaagg
      181 atgccaatga actgatggga aaataccgta tttccggggt cccgattact gtgggaagaa
      241 agcttgtcgg cattattaca aaccgtgatc tgaaatttga aaccgatctt tctaagaaaa
      301 taaaggaacg tatgactgcg gaaggtctga tcacggccat ggaagggatt acgatcgagg
      361 atgcgaagaa gattctggcg aatgcaagaa aagagaagct tccgatcgtt gacaaggatt
      421 ttaatctaaa gggcctgatt acgatcaagg atattgaaaa gcagatcaaa tatccgctgt
      481 cggccaagga tgcgcaggga agactgcttt gcggcgcagg gatcggtatt acggccaatg
      541 tactggaacg tgtggaagcg cttgtaaagg ctaaagtcga tattgttgtt ttggattctg
      601 ctcatgggca ttcggaaaac gtacttcgct gtgcacgcat gatcaaggaa gcttatccga
      661 agctgcagct gatcggcggc aatgttgcta cgagcgaagg cacgaaggca ctgatcgagg
      721 ccggcgtgga tgcggtcaag gtagggatcg ggcctggttc gatctgtacg acaaggatcg
      781 tttccggtat cggtgtaccg caggtgactg cgattatgga ttgctataag gctgcaaagg
      841 agtatgggat ccctgtgatc gccgatggcg gtatcaagta ttcgggagat atgacaaagg
      901 ctttggcggc aggtgccagc gtgtgcatga tgggcagcat ctttgcaggc tgtgatgaga
      961 gtccggggac tttcgaatta tatcagggaa gaaaatacaa ggtctaccgc ggcatgggtt
     1021 ccctttcagc catggaaaac gggagcaagg acagatattt tcaggcggat gccaagaagc
     1081 ttgtgcctga gggcgttgag ggccgtgtgg cttacaaggg aacggtagag gatacggtat
     1141 tccagctgat cggcggtatc cgttccggca tgggctactg cggaacgccg acgatcgaag
     1201 acttaaagca gaacggcagg tttgtaaaga tctccgcagc agctttgaag gaaagccatc
     1261 cgcatgatat ccatattacg aaagaagcgc caaactacag tattgatgaa tagaaaggat
     1321 taaattgatg ggtagttata tagcgaggga gctgaaaaaa gcattaaaaa gaagatatac
     1381 ctatgcttat gttctcggga tactgatttt atgcctgctt gcgaatatcg cggtgatggc
     1441 attccgtacg atttacggta cgaatgaagg gacctatgca tataatctga tagaatatgc
     1501 gacatggtgt tttgtccttc cttactacag ctgtatcttt attgcggata tggggtttgg
     1561 gaaaatttat ccgaatcctc atatcaagga taatgttaca aaaaatctga gcaggacaca
     1621 gatctatctt tccaaactga tcacggaagt tttgctggca atcgtttttc ttgtgatcgc
     1681 gtttattctt ctgatcagta tcaccgcctt gtttcaattg cgggatagaa cgatctgctc
     1741 gtatacgatt tgggatttta cgcagaagat ggtatttgcc acgcctttgt ggatcgccgg
     1801 catttcgttc ggcaacatgt ttctgttcct gtttgaggat aagaggaaag catatgtcgg
     1861 atttttcatt ttgacgtttg ctgttccgcg tattatcatg cagcttgcgg cagagccgtt
     1921 aaagcttgca ccttttaagg taatcagaac atatctgatc acgcagaatt tctctcttct
     1981 gccttatccg gcggatcctg cacgaaatat cccgctgact gttgcgcttg gatttattta
     2041 tgcagcgatc gcgagtgtga tcggagcgat tgtctacaat aaaaagaaaa cagattatat
     2101 cctataaata ataaagcggt cgtattccat aagtgcggga tacggccgct ttgttttaca
     2161 cacattttac attgatttta cattacacgg caaaatttca tgatatagta aaagtggttt
     2221 gcaaatttta catcgatttt gcaaaaacaa tacaaacagt agagtgattg attactggtg
     2281 acggaggaaa ttatgagttt atctgatgta aaaatgatat ttaagtttgt gggaggtctc
     2341 ggtttttttc tgtatgggat gaatcagatg gccgacggtc tgcaaaaatc cgcaggcagc
     2401 agaatgaaaa atctgcttgg ctatctgaca aataacaaat ttatcgcaat tctggtcggc
     2461 acgcttgtga ccgcgatcat tcagagttct tctgcaacga ctgttatggt agtcggtttt
     2521 gtcaatgcgg ggatcatgga tctgacacag gcggtaggcg ttattatggg tgcgaatatc
     2581 ggaacgacgg taacggcgtg gatcgtttcc cttggttcca taggaagtgc gctgaagccg
     2641 gaatttttcg cgccgctgtt agtcgggatc ggcgcgttcg tgcttttgtt cagcaaatcg
     2701 gaaaagaaac gtgatgccgg tgagatcctg gttggttttg gtgccctgtt tgtcggactc
     2761 agctttatgt caggttctat cgaaccatac agggattcgt ctcttttttc cgacgctttc
     2821 cggacgcttg ggcataaccc gattctaggc attctggtcg gccttgtcgt tacgagtatt
     2881 atccagagtt cctccgcatc ggtcggcatt ttgcagacac ttgcgatgaa cggcatggtg
     2941 acatggaatt cagcagttta tattacactt ggacagaata tcggaacatg tgtgactgcc
     3001 ctgatctcca gtatcggtgc agga
//
LOCUS       DAOSYE010000198         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24025_length_3019_cov_3.547571, whole genome shotgun sequence.
ACCESSION   DAOSYE010000198 DAOSYE010000000
VERSION     DAOSYE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24025_length_3019_cov_3.547571"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PLU43_04430"
     CDS             <1..1793
                     /locus_tag="PLU43_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO27688.1"
                     /translation="IGVIPPGTDYIQIGDITRTRIGKIKVLFFAGVNDGIIPSGSGRG
                     GIISDMDREFLQEDGIELAPSSRMQSYTQRLYLYMAMTKPEERLYLSYAKLSEDGRSL
                     RPSYLIKTVREMFVWIKEEKAADVRLISRIWNENAGFRELTEGLQPYIAWRSAGTGTA
                     AGDDMQNYRELFSLYASKPEYGGKLKTLIDTAFLEGVLKRRDSIGRAVAEALYGKELS
                     GSVTRLESYAQCAYQHFLTYGIGLKERELFSFEAKDMGTVFHDALLAYAKLLEKSGYT
                     WFDIPALKAEALIDEAVRRCVASGDYAAVYSSFRTAYMVERIKRITMRTVEVLTGQIR
                     KGSFRPEKFEFVFSAENDYHALNIKLSKEESLRLRGRIDRLDVYEDDTQIYVKIIDYK
                     SGKQSFDLAAIYQGLQLQLVVYLNAAMEQQQGLSAKKEIIPAGILYYHIDDPMIDAGK
                     NDTPEDISRRIVSQLAMKGLVNSSETIIRLMDRDFEKVSDVIPVSLLKTGGLASGSSV
                     ASTEEFKIISDYVNWKIKEMGKGILSGNIMAMPAKEPEACAYCSYRSICHYTGKTDGD
                     GEEDGMERPAKEDVIDRMKENICPKEGTKQ"
     gene            1790..>3019
                     /locus_tag="PLU43_04435"
     CDS             1790..>3019
                     /locus_tag="PLU43_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO27689.1"
                     /translation="MNFTPEQQKAISLRDRNILVSAAAGSGKTAVLVARIIGRIMDKK
                     QPVDIDRLLVMTFTKAAADQMRERIFDAIEEKRKENPFDAHLQKQSALVHHAKITTIH
                     GFCLDILRNHFHEIGLDPDFRVGDEGECRLMKQDVLEKVLEAAYETGEPEFLTMTESL
                     AVKKSDRALETMILKLHEFSMSYPKQEEWLAHCKDMYAAASEDRFEALFWVKGLLADI
                     KQQLNEILERLRQLLMQTEAPEGPYMYRSAIAQDVSSVEQLCMTDRFCSMQRQFSTLA
                     FMTLGRERKDGAFVDPEKKEAVRNGRNQCKKLLQKIAGDFFQDTPKEAVLLLQESSGN
                     VSALADITLAFSRAYAEAKRNKNIVDFNDLEHLSLMVLENGKDTTAREYREFFEEIYV
                     DEYQDSNLVQETLLGLIS"
BASE COUNT          884 a          558 c          861 g          716 t
ORIGIN      
        1 ggatcggcgt gatcccaccg ggaactgatt atatacagat cggagatatt acacgtacga
       61 ggatcggaaa aataaaagtg ttgttttttg caggtgtcaa tgatgggatc atcccatcgg
      121 gcagcggccg tggcgggatc atttccgaca tggacaggga gtttctgcag gaggatggga
      181 tcgaactggc accctcgtcc agaatgcagt cctatacgca aagactgtat ttatatatgg
      241 caatgacaaa accggaagag agactgtatc tgtcttatgc gaaactatcg gaggacggca
      301 gatctctgcg gccctcttat ctgatcaaaa cggtcaggga gatgtttgta tggataaagg
      361 aagaaaaagc ggctgatgtc aggcttattt cccgtatatg gaatgaaaac gcaggattca
      421 gagagctgac ggaaggtctg cagccttata ttgcatggag aagcgccggt accggaactg
      481 cggcagggga tgatatgcag aattaccggg aacttttttc gctgtatgcg tcaaaaccgg
      541 aatatggggg aaaactgaaa acactgatcg acactgcatt tttagaaggc gttctgaaaa
      601 ggcgtgacag tatcggcagg gccgtggcag aggctttata tggaaaggag ctgtcaggca
      661 gcgtaacgag gctggaaagc tatgcgcagt gcgcttatca gcattttctg acatacggga
      721 tcggtctgaa agagcgggag ctgttcagtt ttgaagcgaa ggatatgggg acggtatttc
      781 atgatgcgct tttggcgtat gcaaaactgc ttgaaaaaag cggttataca tggtttgata
      841 tcccggctct caaggcggaa gctctgatcg atgaagcagt ccgccgatgc gtggcgtccg
      901 gagattatgc tgcggtatac agttcatttc ggacagccta tatggttgag cgtataaagc
      961 ggatcacaat gcgtacggtt gaagtactga ccggtcagat ccggaaagga agtttcaggc
     1021 cggaaaaatt cgaatttgta ttttctgcgg aaaatgatta tcatgcttta aatatcaagt
     1081 tgtcaaagga agaatccctt cgtctgcgtg gccggataga ccggctggat gtgtatgagg
     1141 acgatacgca gatctatgtg aagatcatcg attataaatc aggaaaacag tcctttgatc
     1201 ttgctgcgat ttatcagggg ctgcagctgc agcttgtcgt atatctgaat gcggcaatgg
     1261 aacagcagca gggcctttct gcaaaaaagg agatcatacc ggcaggcatt ttatattatc
     1321 atatcgacga tccgatgatc gatgccggga aaaatgatac gccggaggat atcagccgcc
     1381 gtattgtcag tcagcttgca atgaagggac ttgttaacag cagtgaaacg attattcgtc
     1441 tgatggacag ggattttgaa aaagtctctg atgtcatacc ggtctcgttg ctcaaaaccg
     1501 gtggtttggc gtcgggctct tccgtggcga gtacggagga attcaagatc atttcggatt
     1561 atgtaaactg gaagatcaaa gagatgggga aagggatcct aagcggcaat attatggcga
     1621 tgccggcaaa agaaccagaa gcatgtgcat actgcagcta cagaagtatc tgccattata
     1681 cgggtaaaac tgatggtgac ggggaagaag acggaatgga gagacccgca aaagaagatg
     1741 tgatagacag gatgaaagaa aacatctgtc ctaaggaagg aacaaagcaa tgaattttac
     1801 gccggaacag caaaaagcaa tttcattaag agacagaaat attcttgtgt cggctgctgc
     1861 cggatcagga aagacagcgg tgcttgttgc gcggatcata gggcggatca tggataagaa
     1921 gcagcctgtt gatatagaca gactgcttgt gatgacattt acaaaggcag ctgcggatca
     1981 gatgcgggag cggatctttg atgcgatcga agaaaaaagg aaggaaaatc cctttgacgc
     2041 gcatctgcaa aagcagtccg cacttgtgca tcatgcaaaa attacgacga tacatggatt
     2101 ttgtctcgac attttaagaa accattttca tgagatcggg ctggatccgg atttcagagt
     2161 cggtgatgag ggagaatgcc gtctgatgaa gcaggatgtg ctggagaaag tgctggaggc
     2221 tgcctatgaa acaggagaac cggaatttct gacaatgact gagagccttg cagttaaaaa
     2281 gagcgacagg gcactggaga cgatgatatt aaagctgcat gagttttcca tgagctatcc
     2341 gaagcaggag gagtggcttg cgcactgcaa agatatgtat gctgctgcgt cggaagacag
     2401 atttgaagcc cttttctggg taaagggtct tttggcggat ataaagcagc agctgaatga
     2461 aatactggaa agactgcggc agctgcttat gcagacagaa gccccggaag ggccttatat
     2521 gtaccgcagt gcgatcgcac aggatgtttc gtctgtggaa cagctttgta tgacagatcg
     2581 tttttgcagc atgcagagac agttttccac gcttgcattt atgacgcttg gcagagaaag
     2641 aaaagacggt gcgtttgtgg atccggaaaa gaaagaggca gtgaggaacg gcagaaatca
     2701 atgcaaaaag ctgctgcaga agattgccgg tgattttttt caggatacac caaaggaagc
     2761 ggtacttctg ctgcaggaga gcagcggaaa cgtttcggcg cttgcagata tcacactggc
     2821 tttttccaga gcatatgcgg aggcaaaaag aaataaaaat attgttgatt ttaatgatct
     2881 ggagcatctt agccttatgg tacttgaaaa cggaaaagat acaacagcca gggaatacag
     2941 agaatttttt gaggagatct atgttgatga atatcaggac agcaatttgg tgcaggagac
     3001 gctgctcggc ctgatttca
//
LOCUS       DAOSYE010000199         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24030_length_3019_cov_2.479082, whole genome shotgun sequence.
ACCESSION   DAOSYE010000199 DAOSYE010000000
VERSION     DAOSYE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24030_length_3019_cov_2.479082"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1640
                     /gene="xylB"
                     /locus_tag="PLU43_04440"
     CDS             174..1640
                     /gene="xylB"
                     /locus_tag="PLU43_04440"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOO27690.1"
                     /translation="MLYIGVDLGTSAVKLLLMDKGGAIEKIVSKEYPISFPHPGWSEQ
                     KPEDWWTQTLEGLRELTADCDRKEIGGISFGGQMHGLVVLDQEDHVIRPAILWNDGRT
                     GKQTDYLNTVIGKEKLSKYTANIAFAGFTAPKLLWMKENEPELFAKIEKIMLPKDYIA
                     YMLSGVHCTDVSDASGMLLFDVKNKCWSKEMMEICAVKEEQLAKIFESYEVVGTIKPA
                     LAEELDFPKTVKIIAGAGDNAAAAIGTGTVGDGRCNISLGTSGTIFISNKDFCVDKYN
                     ALHSFAHSDGHYHLMGCMLSAASCNKWWMEDIIGTKEYEKEQENITELGENHVFFLPY
                     LMGERSPHNNPDARAMFIGMSMDTTRADMTQAVLEGVAFGIRDSFEVARSLGIKIEKT
                     KICGGGAKSPLWKKIMANVLGIKVEVIESEEGPGYGGAILAAVGCGEYASVEEAAEKL
                     VKVVGAVEPDAELTAKYEKKYQQFKKLYPTVKVLFGSE"
     gene            complement(1604..2767)
                     /locus_tag="PLU43_04445"
     CDS             complement(1604..2767)
                     /locus_tag="PLU43_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOO27691.1"
                     /translation="MITGSKELIRDINSHLVLETILNKGPISRAAISNKLGLTKATIS
                     AIVSDLMENQLIREIGSVDTSLGRKPILLEFCKEHGHIISVDLGVNMISVFTSDLKGN
                     HCGLKQFENNYSRYNIIKGITLLIEQTIRSLPSTAFGVVGICLGIHGVIHENQITFTP
                     YYNYDQLPFAETLEEYFHIPVTLENEANLSVLGEKSFCYHVPNMIGISVHSGIGIGII
                     INNGLYTGFNGNAGEFGHTIVEAYGRDCPCGNKGCLEQYASERAILHDYAKIKGIPNV
                     TIDTFIEKYNAGDSDAADMAEQFIKYISVGINNLLNIFNPEVIVINSTFTMYIPDLIG
                     KIESHLKNRMNKYCTLVPSGLQDTSILLGGVCVCINQFLGVDYSEPNSTFTVG"
BASE COUNT          909 a          561 c          756 g          793 t
ORIGIN      
        1 gtggtggaaa cattgtaact ggggctagcc gtcgcgctag cgacttggta caataacagc
       61 gaagggttct gattgacaaa cgtcattctg tgtagtacgc ttaatccgaa tagttcagtg
      121 actaaactaa taaaagaaat aaccgcaaat ggattggtaa ggaggatttg aacatgttat
      181 acatcggtgt tgatttaggt acttccgcag taaaactgct cctgatggat aagggtggag
      241 caattgagaa aatcgtatca aaggaatatc cgatcagctt tcctcatccg ggatggtcgg
      301 aacaaaagcc ggaggactgg tggacgcaga cgctggaagg cctgcgggaa ttgacagctg
      361 actgtgacag gaaagagatc ggagggatca gctttggggg acagatgcac ggacttgttg
      421 tgcttgacca agaggaccat gtcatcagac cggcgattct atggaatgac ggcagaaccg
      481 ggaaacagac ggattatctg aatacggtga tcggaaaaga aaagctgtca aagtataccg
      541 caaatattgc gtttgcgggc tttacggcgc ccaagcttct ctggatgaag gaaaacgaac
      601 cggaactgtt tgccaaaata gaaaagatca tgcttccgaa ggattatatc gcctatatgc
      661 tttccggcgt acattgtacg gatgtatcgg atgcgtccgg tatgctgctg tttgatgtaa
      721 agaataaatg ctggtcaaag gaaatgatgg agatctgtgc tgtaaaggaa gagcagcttg
      781 caaaaatatt tgaaagctat gaggtcgttg gtacgatcaa gcccgcactt gcagaggaac
      841 tggattttcc gaagacggtg aaaattattg ccggtgcagg cgacaatgcg gccgcagcga
      901 tcgggaccgg tacggtcgga gacggaaggt gcaatatttc acttggcaca agcggaacga
      961 tctttatttc caataaagat ttttgcgttg acaagtataa tgcactgcat tcttttgcgc
     1021 attcggacgg acactatcat ctgatgggct gtatgctttc cgcggcatcc tgcaataaat
     1081 ggtggatgga agatattatt ggtacgaagg aatatgaaaa ggaacaggaa aacattacgg
     1141 agctggggga aaatcatgta tttttcctgc cgtatctgat gggagagcgg tcccctcata
     1201 acaatccgga tgcaagagcc atgtttatcg gcatgtcaat ggatacgacc agagccgata
     1261 tgacacaggc agtgcttgag ggagttgctt ttggtatcag ggattcgttt gaagtagcca
     1321 gatcacttgg aatcaaaata gagaagacca agatctgcgg aggcggagcg aaaagtccgc
     1381 tatggaagaa gatcatggca aatgtgcttg gaatcaaagt agaagtcata gaatctgaag
     1441 aaggacccgg atatggcgga gcgattcttg cggcagtcgg ctgcggtgaa tatgcatctg
     1501 tcgaagaagc tgccgaaaaa cttgtgaagg tcgttggagc agtggagccg gatgcggaac
     1561 tgacagcaaa atatgagaag aaatatcagc agttcaaaaa actttatcca actgtaaagg
     1621 tgctgtttgg ctcagaataa tcaacaccga ggaactgatt gatacagaca cagacacctc
     1681 caagcagtat ggaagtatcc tgaagaccgg atgggacaag cgtgcaatac ttgttcatcc
     1741 ggttttttaa atggctttct atttttccga tcagatcagg aatatacatt gtaaaagtac
     1801 tgttaatgac aatgacttcc ggattgaaaa tattgagcag attattgata ccgacagaaa
     1861 tatatttaat gaactgttcc gccatatcgg ccgcatcgct gtccccggcg ttatattttt
     1921 cgataaaggt gtcaatagta acgtttggga tcccttttat ttttgcatag tcatgtaaaa
     1981 ttgcccgttc cgaagcgtat tgttccagac agcctttgtt gccgcacgga cagtcgcgcc
     2041 cataggcttc tacgatcgta tgtccgaact ctccggcatt tccgttaaat ccggtatata
     2101 agccgttgtt gattattatg ccgataccga taccggaatg cacgctgatc ccgatcatat
     2161 tcggaacatg atagcaaaat gacttttcac cgagtacgga aagattggct tcgttttcaa
     2221 gcgtgacagg tatatgaaag tactcttcca aagtttccgc aaagggaagc tgatcataat
     2281 tgtagtaagg agtaaaggtt atctggtttt catggataac accgtgaatg ccgagacaga
     2341 tgccgacaac gccgaaagca gtggaaggaa ggctgcggat cgtctgttcg atcaataaag
     2401 tgattccttt tataatattg tatcgggaat agttgttttc aaattgcttc agaccacagt
     2461 gattgccttt taaatcggat gtaaaaacag agatcatatt gacacctaaa tcaacggaaa
     2521 taatatgtcc gtgttcttta caaaattcaa gtaaaatcgg ctttcggcca agagacgtgt
     2581 caacgcttcc gatttcccgg atcagctgat tttccatcag atcggacaca atagcggaaa
     2641 tcgttgcttt tgtcagtccc agtttgttgg aaatcgccgc gcgggaaata ggtcctttgt
     2701 tcaatatggt ctctaaaacc agatgagaat tgatatctct gattaactct ttgctgccgg
     2761 tgatcatgat ttgctgctcc ttgccctata agtaatgaaa gataccggaa gcagtaaaac
     2821 aaccggtatt tcatgcgtct aactatatta agtatacaat actaaattta agaagtaaat
     2881 aaaatgaaat atttttattt aacagctgaa attttgtata aacaacttga ctgtataagg
     2941 aacaagtggt agcataaaac tagttagttt agtcattgaa gaaatagtgg aaaggagttc
     3001 ttagcatgaa ggttttgag
//
LOCUS       DAOSYE010000200         3018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24042_length_3018_cov_3.259534, whole genome shotgun sequence.
ACCESSION   DAOSYE010000200 DAOSYE010000000
VERSION     DAOSYE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24042_length_3018_cov_3.259534"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLU43_04450"
     CDS             <1..638
                     /locus_tag="PLU43_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27692.1"
                     /translation="MDNFWQRTMYFNYLSVFETAEVFHNADKVHAGAILTDNYLSDSL
                     SCRLRLPNKYLPALINSRLFGRNLENRAAFRLLSDEHGHGLFGDANGCSDLCYEVNYT
                     GMEETGDAALISLYLNKLLTLCSANNIPVILEQPPMNEASWTNLKESFVNDYTLYMEE
                     LQSDYPEIQINSQIPCYDDQYFGDSSHLNQKGAVLYSHALKIKYSEFFSLQ"
     gene            870..1979
                     /locus_tag="PLU43_04455"
     CDS             870..1979
                     /locus_tag="PLU43_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOO27693.1"
                     /translation="MKKRTLLLLFVLSGILVLAGCGKKEEEEAAAEETVAEPVIQVVT
                     DDSSTETAAAEEESHDGMYRSELTNEWIDESIEDQRPIAAMVDNEKTALPHYGVSTAD
                     IVYEMTNSTENDGVTRFMVLVKDWKNIVQLGSIRSTRPTNLQLIPEWNAVICHDGGPF
                     YIDDYLAKDYVENFSGTFSRVDNGKSREFTEYILAGDLESNFESKGYSETYNDNYPGP
                     HWQFADDASPTDFSSNSEAIDATHIQMPYKHNEPYLDYNADDGLYYYSEYGEAHVDPG
                     NNNTQLSFKNLLLQNARYVEFDENGYMMFHSIDYNRDGYYITNGKALPITWSKDDELT
                     PTKYYDSDGNEIELNTGRTYIALVPDDIWDDLEIE"
     gene            complement(2108..>3018)
                     /locus_tag="PLU43_04460"
     CDS             complement(2108..>3018)
                     /locus_tag="PLU43_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO27694.1"
                     /translation="SAVQFHDDDAVPDINAYTEEEVRQKAGEVRKLLDRYGMAAEFVA
                     PRLWMDPHTIDGGFMSTSKKDREYAMWRAYRSIDIANMLGCDMIVLWLAREGTLCAES
                     KSPVWATKMLIEAINQMLQYDPKIRVCIEPKPNEPIDRSICGTMGHVMAISAATIDPS
                     RVGGNLESAHAILAGLDPSHEIGFALAMGKLMTVHLNDQNGLKFDQDKSFGVENLRQA
                     FNQVKVLKENGYGEHGEYIGLDVKAMRTTTDEDGFQHLKNSLEIFKALEAKVDRFDYD
                     FQKKCVENRDFEGLELYVMKLLMGVD"
BASE COUNT          859 a          733 c          604 g          822 t
ORIGIN      
        1 atatggataa tttctggcag cggacgatgt atttcaatta tctttctgtt tttgaaaccg
       61 ccgaagtctt tcacaatgct gataaggtcc atgccggggc catactgacg gataattact
      121 tatccgactc attaagctgt cgtctgcgtc tcccgaataa atatctgccg gccttgatca
      181 acagccgcct ctttggccgt aaccttgaaa acagggccgc tttccggctg ctttccgacg
      241 aacacggaca cggtctgttc ggtgatgcca acgggtgttc cgacctatgc tacgaggtca
      301 attacacggg tatggaagag accggcgatg cagctctcat cagcctgtac ctgaacaagc
      361 tgcttactct gtgttccgca aacaatatcc cggtcattct ggaacagccc ccaatgaatg
      421 aggcttcctg gacgaacctg aaagaaagct ttgtgaatga ttacacctta tatatggaag
      481 aacttcagtc agattatcct gaaattcaaa tcaacagcca gattccatgc tatgacgacc
      541 agtacttcgg agactcttct catttgaatc aaaaaggggc tgtcctgtat tcacacgcac
      601 tcaaaattaa atattctgaa tttttttcat tgcaatgacc tgattttttc gcagtttctt
      661 aagttttttt aaaaaaatac agattcctct ttacttttat tttgcgatta tgtaaaattc
      721 tcattataat tcattactcg ataacatttt tgtaaaacaa acgtaatatt ttgtacaaaa
      781 taccagtgac ttgccctgta ttttgtgcta taatctacga cagagtaaag tgtaaagtaa
      841 tgcaatcaaa taagggagga attataccta tgaagaaaag aacattgctt ctcctctttg
      901 tattatcggg catccttgtt cttgccggat gcggaaaaaa ggaagaagaa gaggccgctg
      961 ctgaagaaac agttgccgag cctgtcatcc aggtcgttac ggatgactca agtacagaaa
     1021 cagccgctgc tgaggaagaa tcgcatgatg gcatgtacag aagcgaactt acgaatgaat
     1081 ggattgatga atctatagaa gaccagagac cgatcgctgc aatggttgat aacgaaaaga
     1141 ccgctttgcc ccattatggt gtgtctacag cagacattgt ttacgaaatg acgaacagta
     1201 ctgaaaacga cggggttacc cgcttcatgg ttctcgtcaa ggactggaaa aacatcgtac
     1261 agctcggcag tattcgaagt acaagaccta caaacttaca gcttattccc gaatggaacg
     1321 cggtaatctg ccacgacggc ggaccgttct atattgacga ctatcttgcc aaagactatg
     1381 tagaaaactt cagcggtact ttttcacgtg tagataacgg aaaatcccgt gagtttacgg
     1441 aatatatttt ggcaggtgac cttgagtcaa actttgaaag caaaggctac agcgagactt
     1501 ataatgataa ctatccgggt ccgcactggc agtttgcaga cgatgcaagt cctactgact
     1561 tctcttcaaa ttccgaggcg atcgacgcta cgcacataca gatgccgtac aaacataacg
     1621 agccgtatct tgactacaac gcagacgacg gactgtacta ttattcggaa tacggggaag
     1681 ctcacgtaga tccgggcaac aataatacgc agctttcatt caagaatctt cttctgcaga
     1741 atgcacgtta tgtggaattt gatgaaaacg gatatatgat gttccattct atcgactata
     1801 accgtgacgg ctattacatt acaaacggaa aagcactgcc gatcacatgg tcaaaggacg
     1861 acgaactgac accgaccaaa tattatgatt cagatggaaa tgaaatcgaa ctgaatacgg
     1921 gcagaactta tatagcactt gttcctgatg atatttggga tgatctcgaa atcgaataaa
     1981 acagctttat ttaaacaaaa tataagacag ctgctccaac ggatatttct ctgttggagc
     2041 agctgtcttt atcgtttctg gcagtgctcg caaaaataaa cggtgcctcc catttctcta
     2101 aaaataatca gtcgactccc atcaacagtt tcataacata cagctcaagt ccttcaaaat
     2161 ccctgttttc cacacatttt ttctgaaaat cataatcgaa ccgatccact tttgcttcaa
     2221 gtgccttaaa aatttccaga ctgtttttca gatgctgaaa accatcttca tcagtcgtcg
     2281 tacgcatggc cttgacatca agtccgatat attccccgtg ctcgccatac ccgttttcct
     2341 tcagcacctt tacctgatta aatgcctgcc gcagattttc aacaccaaat gacttatcct
     2401 gatcgaattt cagtccgttc tgatcattta aatgaacggt catcagcttt cccatcgcca
     2461 gtgcaaaccc gatttcatga gacggatcca gccccgcaag gatcgcatgg gcactctcta
     2521 agttcccgcc gacacgggaa ggatcgatcg tcgccgcgga tattgccatc acatgtccca
     2581 tcgtaccgca gatgcttcgg tcgatcggct catttggctt tggttcgatg cagacacgga
     2641 tcttcggatc atactgcagc atctgattga ttgcttctat taacatcttc gtggcccata
     2701 ccggagactt ggactccgca cagagcgtcc cctctcttgc cagccacaat acaatcatat
     2761 cacagccaag catatttgca atatcaatgg aacgatatgc ccgccacatc gcatactcac
     2821 ggtctttttt tgaggtgctc ataaatccgc cgtcgatcgt atgcggatcc atccacagtc
     2881 ttggtgccac aaattccgca gccattccat accggtctaa aagcttcctt acctctcccg
     2941 ccttctgtct gacctcttcc tccgtatagg cattgatatc cggcaccgca tcgtcatcat
     3001 gaaactgaac ggccgaaa
//
LOCUS       DAOSYE010000201         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24377_length_2991_cov_3.473093, whole genome shotgun sequence.
ACCESSION   DAOSYE010000201 DAOSYE010000000
VERSION     DAOSYE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24377_length_2991_cov_3.473093"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLU43_04465"
     CDS             complement(<1..1136)
                     /locus_tag="PLU43_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO27695.1"
                     /translation="MAIIRKRVRIGDLLIREGLITEGQLSVALREQKIRNTKLGETLI
                     ALGFVSQDAFSDVLSSQLGIATVNLRKEGLDENAVNLVSEDLMKKNMLIPFAFDEKNP
                     NMLKVAMADPMNIMAIDDVAIMTNMEIIPYFCPSVQISLQLDRLYGKKQAQAAAEQYQ
                     QEHAADLMTEEDSLLNTDVEDAPIVKIVRALLETAVRQGASDIHIEALDREVRVRFRI
                     DGVLQEHMDYDKSLLPALVARIKIISNLDISEKRKPQDGRLTVTVDNKEYDVRVSILP
                     TVFGEKVVMRLTSKDGLTRDKRFLGLTEREMPRFDTIMRNPHGIILVTGPTGSGKSTT
                     CYTVLSELNSEEVNIVTVEDPVEANVDGINQVQVKEDIGMTFAAA"
     gene            complement(1301..2359)
                     /locus_tag="PLU43_04470"
     CDS             complement(1301..2359)
                     /locus_tag="PLU43_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOO27696.1"
                     /translation="MAGKKKKSGVVTICLVITMFFFGFVIGMLLIHFTETAKVEEKLE
                     ETEEEEEQPIVNVYIPERKRLYGVIPVNPYNTDNFRLADGFMAYFDDEGNKISHLGVD
                     LSYHQQTIDWEELKDSACEFVMLRCGYRGYTEGGLIEDEKFEEYAKAANDAVISLGVY
                     FFTQATTVEEAEEEADFVLSLIEDYEISYPVALDTEYVSDNEARTRTADISDELRSEM
                     CIAFCDKIREAGYYPMIYAGENWMRRNMDLDMLQEYDFWAPQYLEENDFMYDFTIWQY
                     TDSGYIQGIDETVDLNISMVDYAQFVPSLREAVLSGGTISNVSSSQSDQESVSADTIS
                     ENAVSVDTISTGSAGENQ"
     gene            complement(2349..>2991)
                     /locus_tag="PLU43_04475"
     CDS             complement(2349..>2991)
                     /locus_tag="PLU43_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27697.1"
                     /translation="LRILKKYLEGHYEVQIENAGYRFVEKMDEYQADLILLDIEMPVL
                     NGLRVFHEFIKNPRNKNIPVVFLSGISEPSVVREVIRHGAAGYFVKTAPKSELLQRVQ
                     QVFDDFKDKHCGGEILVLSNDIALLRKIQNDLEASCYKVYPVSSAFQAMELLRKKHID
                     LMLLGTDVSGAEPEQILAQLSQALAIEQFPALFMEEPYEKEKITEKVGKALGR"
BASE COUNT          783 a          809 c          535 g          864 t
ORIGIN      
        1 gctgccgcga aagtcattcc gatgtcctcc ttcacctgca cctgattgat cccgtccaca
       61 tttgcttcga cagggtcttc caccgtaacg atattgactt cctcggaatt gagttcgcta
      121 agtacggtat aacaggtcgt tgacttaccg cttccggtag gacctgttac aagtatgatt
      181 ccatgagggt ttctcatgat cgtatcaaaa cgcggcatct ccctctcggt aaggccaagg
      241 aaccttttat ccctcgtcag cccatccttt gaagtaagac gcatcacgac cttttcgcca
      301 aatactgtcg gaaggatcga cacacggaca tcatattcct tattatcgac cgttacggtc
      361 aaccgtccgt cctgaggttt tcttttttcg gaaatatcca gattagaaat gatcttaatt
      421 cttgcaacca gtgcgggaag caatgattta tcataatcca tatgctcctg cagcacgccg
      481 tcgatccgga atctgacacg cacttcccga tcgagcgcct cgatatgaat atcactggct
      541 ccctgccgga cagcggtctc taacagcgca cgcacgatct ttacgatcgg agcatcctcc
      601 acatcggtat ttaaaagaga atcctcttct gtcataaggt ctgcggcatg ctcctgctga
      661 tactgctccg cagcagcctg tgcctgtttt ttaccatata accggtccag ctggagtgag
      721 atctgaacac ttggacaaaa atacggaatg atctccatat ttgtcataat ggcaacatcg
      781 tcgatcgcca taatgttcat cggatcagcc attgccacct tcagcatatt gggatttttt
      841 tcatcgaaag caaacggaat cagcatgttt ttcttcatca gatcttcact gacaagattc
      901 acagcgtttt catcaagacc ttcttttcga agattgaccg ttgcaatacc aagctgactg
      961 cttaaaacat cagaaaaggc atcctgtgac acgaacccaa gcgcaatcag tgtctctcct
     1021 aatttggtgt tcctgatctt ctgctcacgc aaagcaacgg acagctgccc ttccgtaatc
     1081 agcccttctc tgatcagcaa gtcaccaatt ctgactctct tccgtataat ggccatagta
     1141 cttctcccac tttcctatgt attcttctgt ttctgccagg atctggcaat atactccgcc
     1201 cgcttatcta tattatatga aaataatgat atggaataca aaataatact taccactttc
     1261 tcattataca cgctttattt tccatgtcaa atatatgctt ttactgattt tcgcctgcgc
     1321 tgcccgtact tatcgtatcc acacttacgg cattttcgct gattgtatcg gcacttacac
     1381 tttcctggtc agactggctc gacgaaacat tggagatcgt cccgccggac agcaccgctt
     1441 ccctaagcga cggtacaaac tgcgcataat cgaccatgct gatatttaag tctaccgttt
     1501 catcaatacc ctgaatatat ccggaatctg tatactgcca aatcgtaaaa tcatacataa
     1561 aatcattttc ctcaagatac tggggtgccc agaaatcata ttcctgcagc atatccagat
     1621 ccatattacg gcgcatccag ttctcacccg cataaatcat cgggtaatac cccgcctccc
     1681 tgatcttgtc acaaaaggca atacacattt cactgcgcag ctcatctgaa atatcggccg
     1741 tacgcgtacg agcctcattg tccgatacat actcggtatc gagtgcgacc ggatagctga
     1801 tctcatagtc ttctatgagg gaaagcacaa agtccgcctc ttcctctgcc tcttcgacag
     1861 ttgtcgcctg tgtaaaaaaa taaacaccaa gcgaaattac cgcatcattt gccgctttcg
     1921 catactcttc aaatttttca tcttcgatca ggccgccttc cgtatacccg cggtaaccac
     1981 agcggagcat gacaaattca catgcggaat ccttcagttc ttcccagtcg atcgtctgct
     2041 gatgataact caaatcaaca ccaagatggg aaattttatt tccttcatcg tcaaaatagg
     2101 ccataaaccc gtctgcaagc ctgaaattat ccgtattgta aggattgacc ggaataacac
     2161 cgtaaagcct cttcctctcc ggtatatata cattgacgat cggctgttct tcctcttcct
     2221 ctgtctcttc cagtttttcc tcgacctttg ccgtctccgt aaagtggatc aggagcattc
     2281 cgataacgaa gccgaaaaaa aacatggtaa taaccaggca tatcgtcacc acacctgatt
     2341 tttttttctt acctgccaag cgccttccct actttctctg ttattttttc tttttcatat
     2401 ggctcttcca taaaaagcgc cggaaactgt tcgatagcaa gtgcctgact aagctgtgca
     2461 agtatctgct ccggttctgc tccggaaaca tcggttccaa gaagcatcag atcaatatgc
     2521 ttttttctaa gcagttccat tgcctgaaat gcggaactta caggatatac tttataacag
     2581 gacgcttcca gatcattctg tattttccta agcagcgcaa tatcgtttga taagaccaga
     2641 atctctccgc cgcagtgttt atccttaaaa tcgtcaaata cctgctgtac acgctgtaaa
     2701 agctccgatt tcggcgccgt tttcacaaaa taaccggcgg caccatgccg gatcacttcc
     2761 cttacgacac ttggctcgga aataccggac aaaaaaacaa caggtatatt tttattacgg
     2821 ggatttttta taaattcatg aaaaacgcga aggccattga gtaccggcat ctcaatatcc
     2881 agaagaatca gatccgcctg atactcatcc attttctcca caaatctgta tcccgcattt
     2941 tctatctgga cttcataatg cccctccaga tactttttta atattctaag t
//
LOCUS       DAOSYE010000202         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24571_length_2976_cov_3.356727, whole genome shotgun sequence.
ACCESSION   DAOSYE010000202 DAOSYE010000000
VERSION     DAOSYE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24571_length_2976_cov_3.356727"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLU43_04480"
     CDS             <1..382
                     /locus_tag="PLU43_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27698.1"
                     /translation="ANRILGYVNAPSMFQLVAARCVDEKTDIAYYERNRNTLYEGLTA
                     LGFTGPKPEGAFYLFMKSPLEDEKEFCKLAKKYHILIVPGSSFACPGYVRIAYCVSYE
                     TIVNSLPKFAELAKELGLQGKETI"
     gene            403..1464
                     /gene="hisC"
                     /locus_tag="PLU43_04485"
     CDS             403..1464
                     /gene="hisC"
                     /locus_tag="PLU43_04485"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO27699.1"
                     /translation="MSWEENIRRVEPYVPGDQPKVTEKIIKLNTNENPYPPSPMVADA
                     IKEFDPDCMRLYPNVTAEPLVKALSQYYGFEEEEIFVGVGSDDVLANCFMTFFNSKKP
                     ILFPDITYSFYDVWTELYRIPYECLPLDQNFRIRKEDYYKENGGIVLANPNAPTGICE
                     SLDFIEDIVRHNPESVVIVDEAYVDFGGESAMPLTKKYENLLVVQTFSKSRSFAGMRI
                     GFAVGNKILIKYLSDIKFSINSYTMNQTAIYYGVKAVEDETYFKETLAKVVATRERSQ
                     EEFVKLGFNYLDSKANFLFVTHKEVPARDIYEYLKTKNIYVRYFSKPRISNFLRVSIG
                     TEEEMDRLFQELGSFLQNK"
     gene            1478..1975
                     /locus_tag="PLU43_04490"
     CDS             1478..1975
                     /locus_tag="PLU43_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOO27700.1"
                     /translation="MDTLVHWFTSSLGQHVSRETVVFIISMIPILELRGGLLASSLLE
                     IPITRAIPICIIGNILPIPFILLFIKRIFIWLKRFRLFRPLIEKLENRAMKKSTSVAA
                     GEFWGLALFVGIPLPGTGAWTGSLIAALLEIDLKKAIIAELVGIMIATVIMSIVSYGL
                     LGAIL"
     gene            2025..2780
                     /locus_tag="PLU43_04495"
     CDS             2025..2780
                     /locus_tag="PLU43_04495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO27701.1"
                     /translation="MEAYTGFAGVYDRFMDNVPYEKWCGYVTGLLKENKITDGLLLEL
                     GCGTGTLTELLAKQGYDMIGIDNSEDMLSIAMDKKAESGQEILYLLQDMREFELYGTV
                     RAVISICDSMNYITEAEDLLQVFCLVNNYLDPGGLFLFDLNTVYKYRELLADHIFAEN
                     REDCSFIWENEFDEETMLNEYLLTLFIREKEKNCYQKFDEVHYQRAYELAQIRELLNE
                     SGLRFMAAYEAFTKDPVRNDSERIYVLAKKPVD"
     gene            2802..>2976
                     /locus_tag="PLU43_04500"
     CDS             2802..>2976
                     /locus_tag="PLU43_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOO27702.1"
                     /translation="MEDYIVRATAGNGQIRAFAAATRCLVETARQLHQTSPVVTAALG
                     RLLTAGAMMGAMQK"
BASE COUNT          827 a          552 c          792 g          805 t
ORIGIN      
        1 tgcaaaccgt attctaggct atgtcaatgc accgtccatg ttccagctcg ttgcagccag
       61 atgtgtggat gagaagacag acattgccta ttatgaaaga aaccggaata ccctgtatga
      121 gggacttaca gcccttggct ttacgggacc gaagccggaa ggtgcgttct atctgttcat
      181 gaaatcgccg cttgaggatg aaaaggagtt ctgcaaactg gcaaagaagt atcatatcct
      241 gatcgtaccg ggctccagct ttgcgtgtcc gggatatgta cgtattgcgt attgcgtgtc
      301 ctatgagacg atcgtgaatt ccctgccgaa gtttgcggag cttgcaaagg agctcggact
      361 gcaggggaaa gaaactatat agaagtaagg agactgactg aaatgagctg ggaagagaat
      421 ataaggcgcg tagagccata cgtacctggt gatcagccga aagttaccga aaagatcatc
      481 aagctgaaca caaatgagaa tccatatccg ccgtcgccga tggtcgccga tgccataaag
      541 gaattcgatc cggactgtat gcgtctgtat ccgaatgtga cggcagagcc tcttgtaaaa
      601 gcgctttcgc agtattatgg atttgaagag gaagaaatat tcgttggtgt cggttcggac
      661 gacgtactgg ccaattgctt tatgacattt tttaattcca aaaagccaat attatttccg
      721 gacatcacct attcttttta tgatgtctgg accgaattgt accggatccc atatgagtgt
      781 ctgccgcttg accagaattt cagaataaga aaagaggatt attataagga aaacggaggg
      841 atcgtgctgg ccaatccgaa tgcgccgaca ggcatttgcg aatctttgga ttttattgag
      901 gatattgtaa ggcataatcc ggagtccgta gtcattgtcg atgaggcgta tgtggatttt
      961 ggcggagaat ctgccatgcc gcttacgaaa aaatatgaga atctgcttgt tgtacagacc
     1021 ttttcgaagt cccgttcatt tgccggtatg agaattgggt ttgcggtggg aaataaaatc
     1081 ctgattaaat atttgagcga catcaaattt tccatcaatt cctatacaat gaatcagacc
     1141 gcgatttatt atggagtcaa ggctgttgag gatgaaacct attttaaaga gacgcttgcg
     1201 aaagtagtcg cgacaaggga acgcagccag gaggagtttg ttaaacttgg atttaattat
     1261 ttggattcca aagcgaattt tttgtttgtc acacacaagg aagtaccggc cagggatatt
     1321 tatgaatatc tgaaaacaaa aaatatttat gtcagatatt tcagtaagcc caggatctcg
     1381 aattttctgc gtgtttccat tggcacagaa gaggaaatgg acagactgtt tcaggagctt
     1441 gggagttttc tgcaaaataa atgaaaggta gaaatatatg gacacacttg tacactggtt
     1501 tacttcttct cttggtcagc atgtttcaag agaaacggtc gtatttatta tttccatgat
     1561 tccgatcctg gagctgagag gaggactgct tgcgtcttct ctgctggaaa taccgatcac
     1621 gagagcgata ccgatctgta tcattgggaa tatattgccg attcctttta ttctgttatt
     1681 tatcaagcgg atctttatat ggctgaaacg atttcggctg ttcagaccgc tgatcgagaa
     1741 gctggaaaat cgcgcaatga aaaaaagcac ttccgtggca gcaggagagt tttggggact
     1801 ggcgctgttt gtcggaattc cgcttccggg aacaggcgca tggacaggtt cactgatcgc
     1861 agcgcttttg gagatcgatc tgaaaaaagc gatcattgcg gagctggtcg gcattatgat
     1921 cgcgacagtg atcatgtcga tcgtgtctta cggtctgctg ggagcgatcc tgtaggcagt
     1981 tgccgtggac cgactgatga gataattaca gggatgtgtg gattatggaa gcttataccg
     2041 ggtttgccgg ggtctatgac cgttttatgg acaatgttcc ttatgaaaaa tggtgcggtt
     2101 atgtgaccgg actcctgaaa gaaaataaaa taacagatgg tttgctattg gagcttggct
     2161 gcggaaccgg tacattgacc gaattgctgg caaaacaggg gtatgatatg atcggtatcg
     2221 ataattctga agacatgctt tcgatcgcaa tggacaaaaa agcagaatca gggcaggaga
     2281 tcctttatct gctgcaggat atgcgggaat tcgaattata tggaacagta cgcgcggtga
     2341 tcagcatctg tgacagcatg aattatatta cggaagcaga agatctgctg caggtatttt
     2401 gtcttgtaaa taattatctg gacccgggag gactgtttct ttttgatctg aataccgttt
     2461 ataaatacag ggaactgctg gcggatcata tttttgcgga aaaccgtgag gactgcagtt
     2521 ttatctggga aaatgaattt gacgaagaga caatgctgaa tgagtatctg ttaacactct
     2581 ttattcggga aaaagaaaag aactgttacc aaaaatttga tgaggtgcat tatcagcgtg
     2641 cttatgagct ggcacagatc agagagcttc tgaacgaatc aggactgcgg tttatggccg
     2701 cctatgaggc gtttacgaaa gatccggtga gaaatgacag tgaacggatc tacgtacttg
     2761 caaagaagcc tgtggattga tggtcggatg aggtgataat gatggaagat tatattgtgc
     2821 gcgcaacggc cggaaacggt cagatccggg catttgcagc tgcgactcgc tgtcttgtgg
     2881 aaacggcacg gcagcttcat cagacgagtc cggtcgtaac ggcggcactt ggcaggcttt
     2941 tgactgcagg tgcaatgatg ggtgcgatgc agaagg
//
LOCUS       DAOSYE010000203         2975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24584_length_2975_cov_3.001712, whole genome shotgun sequence.
ACCESSION   DAOSYE010000203 DAOSYE010000000
VERSION     DAOSYE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24584_length_2975_cov_3.001712"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLU43_04505"
     CDS             <1..576
                     /locus_tag="PLU43_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO27703.1"
                     /translation="LLIFDEIQTGIGRTGTMFAYQGYGVEPDILTSAKALGGGIPLSA
                     VIGKKKYMEQWVPGAHGGTFGGNPLACAAALETLQVIEEENLLDNCRQMGSYLKDRSR
                     TLQSRYPDLIGDVRGVGLMVGIELITEGNLPNPQMASKIKEKALEKDLLILLCGSEHN
                     IIRFIPPLNVSKEELDFAMEVIENVFEEIVE"
     gene            736..1491
                     /locus_tag="PLU43_04510"
     CDS             736..1491
                     /locus_tag="PLU43_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27704.1"
                     /translation="MASVSSISSSTDYSTLFESLSSSSSSSSSSSTLSTDYLADYASI
                     ENGSYAKLAKAYYGDSSDSTTVTSAEAEDTIKANKILKSYSDDLRSSASALADSKTLF
                     TNTVETTDDDGNTTTDYDYDAIYSALESFVDDYNNVIESGGDSENSSILRSTLSMTEM
                     TEANESLLSSVGITVNDDNTLSIDEDTVKEADINDLKALFSGTGSYADSIAEKASAIS
                     YKANYENNKLSNYTSSGTYSTSDSVGSIYDSSY"
     gene            1553..1636
                     /locus_tag="PLU43_04515"
     tRNA            1553..1636
                     /locus_tag="PLU43_04515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Leu,seq:cag)
     gene            1758..2354
                     /locus_tag="PLU43_04520"
     CDS             1758..2354
                     /locus_tag="PLU43_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO27705.1"
                     /translation="MQFQKHEIKDRILTAARDEFLKNGFEKASIRKITAAAKTSKSNV
                     YNYFHNKDELFIAVVTPAISEIKAGFEELQIQNTGIHARTYSITAQKEVIRKIMEFVY
                     RHDIDIKLLLFRSSGSSLSGFKDRVTDMLSVLLISWITHAAPDKEISEFFIRSVAGFY
                     IGVIEQMLSQGLTREQAAEHFEVFLKFVYGGWNTVLTS"
     gene            2394..>2975
                     /locus_tag="PLU43_04525"
     CDS             2394..>2975
                     /locus_tag="PLU43_04525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO27706.1"
                     /translation="MKSSGKRIEEKPSRTAAWTCTCRALSYLESEPQYKSEDYIAPEL
                     LPKFLLVLFKIKLFRKLFKNILIPSGMYEYVIARTKYFDFVFENVIANGFEQILIFGA
                     GYDTRSIRFLSGGQNIKIYELDAPNTQNAKISQLKKRGITVHPNVVFVPIDFNRESLK
                     DKLIEAGFEKGKKSLFMLEGLTMYLDQKAVDSTF"
BASE COUNT          946 a          532 c          704 g          793 t
ORIGIN      
        1 cttctgatct ttgacgagat ccagacagga atcggccgga ccggcacgat gtttgcttac
       61 cagggatatg gggtggagcc ggatatcctg acgtctgcca aagcgcttgg cggcgggatc
      121 ccgctttctg ctgtcattgg aaaaaagaaa tatatggagc agtgggtacc gggtgcgcat
      181 ggagggactt ttggaggaaa tccgctggca tgtgcagctg cactcgaaac actgcaggtg
      241 atcgaagaag agaacctgct tgacaactgc agacagatgg gcagctattt gaaggatcgt
      301 tccaggaccc tgcagagccg ttatccggat ctgatcggag atgtgcgcgg tgtggggctt
      361 atggtcggaa tagagctgat tacggaagga aatcttccga atcctcagat ggcttcaaaa
      421 ataaaagaga aagcgctgga aaaggatctt cttattttgc tgtgcggaag cgaacacaac
      481 atcatacgtt ttattccgcc gttgaatgtg tcaaaggaag aactggattt tgccatggaa
      541 gtaatagaaa atgtttttga agaaatcgtg gagtaaacgg ttaattttct gtgaaaactg
      601 tccgatattt atagcagagt attgtgaaag cagtttaatt ctgctaaaat gaaactgaat
      661 atgaggggat ttacgattgc agtaacccat taacagacag gcatggatgc cgacaaattc
      721 aaaggagtga ttcacatggc atcagtttcg agtatcagtt cttcaacaga ttattcaacg
      781 ttatttgaaa gtttatcttc aagctcttcc agcagttcct ccagcagcac gttaagcacc
      841 gattatcttg cggattatgc aagcattgaa aatggcagct atgcaaaact ggccaaggca
      901 tattacgggg acagttcaga cagcacgaca gtcacatccg ctgaggcaga ggatacgatc
      961 aaggcaaata agatcctcaa atcgtactcg gatgatttaa ggtcttctgc cagcgcactt
     1021 gccgacagta aaacgctgtt tacaaatacg gtagagacga cggatgacga cggaaataca
     1081 acaacagatt atgattatga cgcgatttac agtgcactgg aatcatttgt ggatgattac
     1141 aataatgtga tcgagtccgg aggagattcg gaaaacagtt cgatcttaag aagcacctta
     1201 tccatgacgg agatgacaga ggcaaacgaa agtctgctat catctgttgg tattacggtc
     1261 aatgacgata atacgctttc catagatgag gatacggtaa aggaagcgga tatcaatgat
     1321 ctgaaggcat tgttcagcgg cacgggttct tatgcggaca gtatcgcgga gaaagcatcg
     1381 gctatttcgt acaaggcaaa ttatgaaaat aataagctga gcaattatac gtcaagcggt
     1441 acttactcaa catctgactc ggtcggaagt atttatgata gttcttattg acaaatgaag
     1501 aaattcatta taaaatatca atcgtacggt ttgccagtga cagattgtat gcgcaggaat
     1561 ggcggaattg gcagacgcgc acggttcagg tccgtgtgat agcaatatca tgagggttca
     1621 agtccctttt cctgcacgat aaaaaagtag agaaatcgca gttttcgcgg tttctctttt
     1681 tttttcttga caaaaagagc acaaacaggt acagtgtact tataagtaca ctgtacctgt
     1741 ttgcgaaagg agacattatg caatttcaaa aacatgaaat aaaagatagg atacttacgg
     1801 ctgcacgtga tgaatttttg aaaaacggat ttgagaaagc atctattcga aaaataacag
     1861 cagccgcaaa aacttcaaaa agtaatgtgt ataattattt tcacaataaa gacgaacttt
     1921 ttattgcggt agtaacccct gctatatcag aaataaaagc cggctttgaa gaactgcaaa
     1981 ttcaaaacac cggcatacat gcgagaacct attctattac agcgcaaaaa gaagttatcc
     2041 gaaaaatcat ggagtttgtg tacagacatg atatcgacat aaaactgctt ttgttccggt
     2101 catcgggctc ttccctttcg ggatttaaag accgggtaac cgatatgttg tctgttttgt
     2161 taatcagctg gattactcat gccgctccgg acaaagagat ttcagagttt tttattcgtt
     2221 cagttgcagg attttatata ggggtaattg aacagatgct ttcacagggt ctgaccaggg
     2281 agcaggcagc cgaacatttt gaggtatttt taaaatttgt atacggcggc tggaatacgg
     2341 tattaacgag ctgataagta ctgttgattg aagtagaagg aggatgccat tacatgaaaa
     2401 gcagcggaaa aaggatcgaa gagaagccat caaggacagc agcatggact tgcacatgcc
     2461 gggcactttc atatcttgaa agtgagccgc aatataaaag tgaggattat attgcaccgg
     2521 aactattacc gaaatttctg cttgtattat ttaaaataaa gctttttaga aaattgttca
     2581 aaaatatact gataccaagt ggaatgtatg aatatgtaat tgcacgcaca aaatattttg
     2641 attttgtatt cgaaaatgta attgcaaacg gttttgaaca aatacttatt tttggtgcag
     2701 gttatgatac acggagcatc cggttccttt ccggtggtca aaatatcaaa atttatgaac
     2761 tggatgcgcc aaatactcag aatgcaaaga tcagtcagct gaaaaaaaga ggcattacag
     2821 tacatccgaa tgtggtgttc gttccaattg attttaacag agaatcactg aaagataaac
     2881 tgatcgaagc cgggtttgaa aaaggaaaga aatctttgtt tatgctggaa ggcctgacaa
     2941 tgtatttgga tcagaaagcc gtcgactcga cgttt
//
LOCUS       DAOSYE010000204         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24603_length_2974_cov_2.422405, whole genome shotgun sequence.
ACCESSION   DAOSYE010000204 DAOSYE010000000
VERSION     DAOSYE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24603_length_2974_cov_2.422405"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PLU43_04530"
     CDS             complement(<1..1152)
                     /locus_tag="PLU43_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27707.1"
                     /translation="MNLDMTKGSPLKLITKFTLPLIIGNVFQQFYNMVDTVIVGRFVG
                     VEALAAVGATGTIMFLIFGFMSGITTGFTVLTSQKYGAGDITGVKKSVGSAAILSVII
                     TIFATICSIGGMRRLLLLLNTPSDIFEMSYEYIVIICAGMVCCVLYNMMSSLLRAVGN
                     SVVPLVFLIISAVLNIFLDLLLIINYQMGVAGAAVATVVAQGVAGLACVLYVAKKVPL
                     LCIKGEHLHLHGEFARTQMRIGVPMALQFSITAIGTLILQGALNMLGSIAIAAYTAAS
                     KAEQLLTQPFLAIGMTMATYSAQNYGIYDFKRIRQGTKISCMMTSVYAVIIAFAANKL
                     IASIIRLFVTGDIDDIIVYGSTYIQVVSLFFIPLGLIFVFRNVMQGMGFG"
     gene            complement(1381..2613)
                     /locus_tag="PLU43_04535"
     CDS             complement(1381..2613)
                     /locus_tag="PLU43_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOO27708.1"
                     /translation="MRMTQTQLDMLKVQLKKVMKNSPFYQEKFKGINVDGITCQEDFE
                     KLPFTWKGDLREAYPLGLMAVPEKEIVRIHSSSGTTGTPIIIPYTQKDVDDWADMFAR
                     CYRFAGITNMDRIQITPGYGLWTAGIGFQTGAERLGAMVVPMGPGNTDKQLRMMMDLK
                     TTVLCATSSYALLLAEEIAKRGIGTHIHLKKGVIGSERWGDKMRSRIAKELGVSLYDI
                     YGLTEIYGPGIAINCDHSKAMHYWDDYMYIEILDPHTGEPVEDGEIGEIVITTLVKEG
                     APLIRYRTHDLSRFEPGDCPCGSKHPRIDTLIGRTDDMVKVKGCNIFPSQIEDVIKDV
                     EGASSEYQVMIDHLGGKDILTIFIEVKKGAGKTDVAHELSSLFKIKIGMTPLVKPVDI
                     GELPRSEKKSRRIFDNRY"
     gene            complement(2662..>2974)
                     /locus_tag="PLU43_04540"
     CDS             complement(2662..>2974)
                     /locus_tag="PLU43_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO27709.1"
                     /translation="TAIPPVTASLSADLYVGEEMLAYLKKHVKRLIIVDGDAVCEECG
                     SSRVLNIVLLGAAIATGELDIGIDEMEQTIDAMVNEKYHEMNRKALFAGAELAGSMQV
                     Y"
BASE COUNT          802 a          807 c          562 g          803 t
ORIGIN      
        1 gccaaagccc attccctgca tcacatttct gaaaacaaag atcagaccaa gcggtataaa
       61 aaagagggat acgacctgta tataggtgct tccgtaaact atgatatcat cgatatctcc
      121 ggtaacgaac agcctgatga tcgacgcgat cagtttgttt gcagcaaatg caataatgac
      181 tgcatagacc gaagtcatca tacagctgat cttcgtcccc tgccggatcc ttttgaaatc
      241 atatatcccg taattctgtg cactgtaagt cgccattgtc atcccgatcg ctaaaaacgg
      301 ctgtgtcagc agctgttccg ccttgctcgc ggccgtatat gcggcgatcg cgatcgaacc
      361 taacatattg agcgccccct gcagaatcaa agtaccaata gcagttatgg aaaactgcaa
      421 tgccatcggc acgccgatcc gcatctgtgt tctggcaaat tctccatgca gatgcaggtg
      481 ctctcctttt atgcaaagca gcggcacctt ttttgccaca tagagcacac acgcaagacc
      541 tgcaacgccc tgcgccacta cggtggccac agccgctcct gccacaccca tctggtaatt
      601 tatgatcagc agcaggtcga gaaagatatt caatacggca gaaatgatca gaaatacaag
      661 cggtacaaca ctgtttccaa ccgcgcgcag cagactggac atcatattat aaagcacaca
      721 gcagaccatg cccgcacaaa tgatcacaat atattcatag gacatctcaa aaatatccga
      781 cggcgtattc agtaaaagca gcagccgacg cattccgccg atactgcata tggtagcaaa
      841 gatcgtgatg atcacagaca ggatcgccgc agaaccaaca ctcttcttta cacccgtaat
      901 atcaccggca ccgtattttt gcgacgtcag taccgtaaag ccggttgtga tcccgctcat
      961 aaatccgaaa atcaaaaaca tgatcgttcc tgtcgcacca acggcagcca gcgcctccac
     1021 tccgacaaaa cggccgacaa tgaccgtatc taccatatta taaaactgct gaaaaacatt
     1081 tcctatgatc agcggcaatg taaactttgt aatcaatttc aacggtgaac ctttcgtcat
     1141 atccaaattc atatctgttc tctccttaca ggcaaaagtt ccggtcaaaa taaacggctt
     1201 ccaaaccgtt tgaatatcat acaatacgaa ctatgcttcg tcaagaacat aatctctata
     1261 tttttcgtac atttctacct aatcatcagt atctttttta cctgccggac agtgcatctg
     1321 taaaacacaa aaaagaaccc gcttctgcaa ggttctttct atgtatatag catattttat
     1381 ttaataccgg ttatcaaaaa ttcttcttga ttttttttcg cttcttggca gttcaccaat
     1441 atcgaccggc ttcacaagag gtgtcattcc gatcttgatc ttgaacagac tggacagctc
     1501 gtgtgcaaca tccgtcttgc ccgcaccttt cttcacttca ataaaaatcg tcagaatgtc
     1561 ttttccgcca agatgatcga tcatgacctg atattcactt gacgcacctt cgacatcctt
     1621 tataacatct tcgatctggc tggggaaaat attgcagcct tttaccttga ccatatcatc
     1681 cgtcctgccg atcagcgtgt cgatccgcgg atgtttactg ccgcatggac agtcgcccgg
     1741 ctcaaatctg gaaagatcat gcgtacgata acggatcagg ggcgctcctt cctttacaag
     1801 tgttgtaatg acgatttccc cgatctcccc gtcctctaca ggttctcccg tatggggatc
     1861 cagaatttca atatacatat aatcatccca gtaatgcatt gccttgctgt ggtcacagtt
     1921 tatggcaatt cccggaccat agatctccgt cagcccgtaa atgtcataaa ggctcacgcc
     1981 cagctccttt gcaatacggg aacgcatctt atcgccccat cgttccgaac cgatcactcc
     2041 ttttttcaga tggatatgtg taccgatccc gcgttttgcg atttcctccg caagcagcag
     2101 cgcataagaa gatgtggcac ataataccgt agtctttaaa tccatcatca tccgaagctg
     2161 tttatccgta ttaccgggcc ccattggaac gaccattgcc ccgagtcttt ccgcaccggt
     2221 ctgaaatccg atccctgcag tccaaagacc gtaccctggt gtgatctgga tcctgtccat
     2281 attggtaatg cccgcaaacc ggtagcatct tgcaaacata tcagcccagt cgtcaacatc
     2341 tttctgcgta taaggaataa tgatcggcgt ccctgttgtt ccagaggaag aatggatcct
     2401 gacgatctct ttttccggca ctgccatcag gccaagcgga taggcttctc tcaagtcccc
     2461 tttccatgta aaaggcagtt tttcaaaatc ctcctgacag gtgattccgt ctacattgat
     2521 tcctttgaat ttttcctgat aaaagggact gttcttcatc accttcttaa gctgtacttt
     2581 cagcatgtca agctgggtct gtgtcattct cattattttt cctcttttct ttataatgca
     2641 aaacaatacg gctgtaaata atcaatatac ctgcatggaa ccggcaagtt cggccccggc
     2701 aaacagagct ttccgattca tttcatgata tttttcatta accattgcat caatcgtctg
     2761 ttccatttca tcaattccaa tgtcaagctc tcccgttgcg atcgcagcgc caagaagcac
     2821 aatattcaga acacgggaag aaccgcactc ctcacaaacg gcatctccat ctacgataat
     2881 cagccgtttt acgtgttttt tcagataagc aagcatttcc tctccgacat acagatccgc
     2941 gcttaacgaa gctgtgaccg gggggattgc cgtt
//
LOCUS       DAOSYE010000205         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24638_length_2971_cov_4.035665, whole genome shotgun sequence.
ACCESSION   DAOSYE010000205 DAOSYE010000000
VERSION     DAOSYE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24638_length_2971_cov_4.035665"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLU43_04545"
     CDS             <1..1305
                     /locus_tag="PLU43_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO27710.1"
                     /translation="QFSVISICIAGCLVWDAINGPLMGVIIENSHLKHGKFRPWIMTG
                     ALLNAVIIVILFSVRPKGWLFVLFFGIGYLLWGMTYTMNDIAFWGMLPSLSSDPQVRN
                     TLVTLMSIFICIGQFTVAGVLPVIVAGNAVAAYRGAAFVVALCFIGFQLLTFLGVTER
                     PREEAKEKLSLKGMLLVFKRNDQLIPIGIASFLFNIGSNLLIVFAVNFFYFEFGYSQG
                     GTLIFLFTVMYGLGTLFSQAAFSIISKHFSRMHILRFCTTVIAAGYLLLLSFGYVLPK
                     NIILLNVIGFLIFFCQGLYNLVVVVMLNNTIEYDEYRFHERHDSVISAVRSFSVKFAG
                     AVNQGLSALVLIVSGIYSISQKISGLEIEVGKQTLTQEDALVQANGYIAEVLPYQTLV
                     LRLGMVLIPILILTGCYFLIKTKYKIDEAEYDRIVKEIEQRR"
     gene            complement(1302..1988)
                     /locus_tag="PLU43_04550"
     CDS             complement(1302..1988)
                     /locus_tag="PLU43_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOO27711.1"
                     /translation="MKIVTIYGQQHKGNTYRLTQLLLSELNTLFKKSDTPEIKEFYVN
                     GTSQCLGCAQCIMKDEELCPHREEIYPVLSAIEAADLIILASPNYCMNMSGQMKTFCD
                     HMAYRWMAHRPFDMRQKTGAVISTAAGSGAGKVTKLLKEQLQWWSVGHVFRLPQIIWD
                     MNWEDGSYPQMKKLKKKVRKTAGRIYKNMCRPRTEYKLSFLFFIMKNMHKGTMCPPFE
                     REYWKEQHLI"
     gene            2180..2662
                     /locus_tag="PLU43_04555"
     CDS             2180..2662
                     /locus_tag="PLU43_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27712.1"
                     /translation="MEEHMIQARDIDLNSETHLDEMIIPDIKPEKNASGKPSIVVIDD
                     DFSTLDLMKIYLQRNYDYIAFDSPREAIFYLNRHIPDLIFMDCYIRMIKSRRIIEIIH
                     SYKELAGVPIVYIAEPSEKGAVMARSRSTVVDCITRPVARGDLQLVLDRVFIKNDNNE
                     "
     gene            2803..2876
                     /locus_tag="PLU43_04560"
     tRNA            2803..2876
                     /locus_tag="PLU43_04560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2837..2839,aa:Arg,seq:acg)
BASE COUNT          756 a          567 c          741 g          907 t
ORIGIN      
        1 cagttcagtg tgatttccat ctgcatcgcg ggctgccttg tatgggatgc tattaatggc
       61 ccgctgatgg gcgtgattat tgagaacagt catttaaagc atgggaaatt ccggccgtgg
      121 attatgaccg gtgcattgct gaatgcagtc atcatcgtta ttttattttc ggtacggcca
      181 aaaggctggc tttttgttct ttttttcggg atcggctatc ttttatgggg aatgacgtat
      241 acgatgaatg acattgcttt ttggggcatg ctgccaagcc tgagcagcga tccgcaggta
      301 cggaataccc ttgtgacatt gatgagcatc tttatctgca tcggtcaatt tacggtagcg
      361 ggggtacttc ctgttattgt ggcgggaaat gcagtagcgg cttacagggg ggcggcgttt
      421 gttgtggcgc tttgttttat cggctttcag ctgctgacat ttctgggtgt gacggagcgg
      481 ccgcgggaag aggcaaagga aaagctgtcg ctgaaaggaa tgcttttggt gttcaaaaga
      541 aacgaccagc tgataccgat cggaatagcg tcctttcttt ttaatatcgg aagcaatctg
      601 ctgatcgtgt ttgccgtcaa ctttttttac tttgaattcg gttattcaca gggcggtacg
      661 ctgatctttc tttttactgt aatgtacggc cttggaacac tcttttcgca ggcagcgttt
      721 tccataattt caaagcattt ttcaagaatg catattttac ggttctgtac aacggtcatt
      781 gcggcaggct atttgttatt gctgtctttt gggtatgtac tgccgaaaaa tatcattttg
      841 ctgaatgtga tcggattttt gatttttttc tgccaggggc tttataacct cgttgttgtt
      901 gtgatgctga acaatacgat cgaatatgac gaatatcggt ttcacgaacg gcatgacagc
      961 gtgatctccg cggtccgttc gttcagtgta aaatttgcag gtgcagtcaa ccagggcttg
     1021 tccgcattgg ttttgatcgt cagcgggatc tattcgatca gtcagaagat cagcggactt
     1081 gaaatcgaag ttggaaagca gacgttgacg caggaggacg cgcttgtgca ggcaaatggg
     1141 tatattgcgg aagtactgcc ttaccagacg cttgtgcttc ggctcggcat ggtgctgatc
     1201 ccgattttga ttttgacagg ctgttatttt ctgatcaaaa ccaaatataa gatcgatgaa
     1261 gcggaatatg acaggatcgt aaaagaaatc gagcagcgcc gttagatcag gtgctgctct
     1321 ttccaatatt ctctttcaaa aggcggacac atcgttcctt tgtgcatatt cttcattata
     1381 aaaaacaaaa aactgagttt atattctgtt ctgggacggc acatgttttt ataaatcctg
     1441 ccggcagttt tgcggacttt ctttttcagc tttttcattt gaggatagga tccatcctcc
     1501 cagttcatat cccagatgat ctgcggaagc cggaatacat gtccgacaga ccaccattgt
     1561 aattgttctt tgagcagttt ggtaactttg ccagcgccgg atccggcagc cgtggaaatt
     1621 actgcacctg ttttttggcg catatcgaac gggcggtggg ccatccagcg gtatgccata
     1681 tgatcacaga aggttttcat ctgaccggac atattcatac agtaattcgg ggaggcgagg
     1741 atgatgagat cagctgcttc aatagccgac agaacaggat aaatctcttc tctgtgagga
     1801 cagagctctt catctttcat aatacactgt gcacagccga ggcactggct ggtgccgttc
     1861 acgtaaaatt cttttatttc aggtgtatct gattttttaa aaagcgtatt taattcggaa
     1921 agaagcagct gtgtgagccg gtacgtattg cctttatgct gctgtccgta aatcgttacg
     1981 atcttcattt atctgtgtcc tttcgttttt ttcattatac tgttttgaaa aaaaatagtc
     2041 aaagaatatg ttccttcgga tacgtctgtt tttacaggtc atgaggcgga attgacactt
     2101 ttatttcaaa gaaaggtgtg ataaaattta tagtaaagaa aaaacacggg atgatattta
     2161 tgtttaggga gattacataa tggaagaaca tatgattcaa gcgcgcgaca ttgacctgaa
     2221 cagcgaaact catttggatg agatgatcat tcctgatatc aaaccagaaa aaaatgcttc
     2281 agggaaaccc tcgattgttg tgatcgatga tgatttttca acattggatc tgatgaagat
     2341 ctatctacag agaaattacg attatatagc ttttgacagt ccgagagaag ctatttttta
     2401 cttgaacaga catattccgg atctgatatt tatggactgc tatatcagaa tgataaaaag
     2461 caggcgtata atcgaaatta ttcattctta taaagagctt gcgggtgtgc cgatcgttta
     2521 tattgcggaa ccgtctgaaa aaggagcggt tatggcgcgg agcaggtcca ctgttgtgga
     2581 ctgtattacg cgtcctgttg cgcggggaga tctgcagctg gttttggatc gtgtatttat
     2641 caaaaatgat aataatgaat aaggaaaaga cattccggaa agtttgtggg aaagacaggc
     2701 acagcgctcg gaaccgacag ggttttgaac tgcagaaacc gcttgactta caatacgcgg
     2761 tatggtaaga taaatgacgt cgcaaagcag tttgcgataa ttgtgcgtgt agctcagctg
     2821 gatagagcgt ctggctacgg accagaaggt cgggggttcg aatcctctca cgcacgtatt
     2881 ggaaaccctg taaatagcat gtttacgggg tttttatttt agtgcgcctg gcggcgcacg
     2941 tttctaactg gtgaaagtcc agaatccgcc c
//
LOCUS       DAOSYE010000206         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24674_length_2969_cov_2.725806, whole genome shotgun sequence.
ACCESSION   DAOSYE010000206 DAOSYE010000000
VERSION     DAOSYE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24674_length_2969_cov_2.725806"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLU43_04565"
     CDS             <1..335
                     /locus_tag="PLU43_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27713.1"
                     /translation="KQAKGLNVAGYLTKPVSQSSLYDAIVRLIGSGMENDTVRSSRME
                     KDYQLAGLKVLLAEDNEINQQVAAELLSSQGITVDIVWNGSQEDGSCYSGYCHDCTDY
                     GRRKGSMF"
     gene            265..1083
                     /locus_tag="PLU43_04570"
     CDS             265..1083
                     /locus_tag="PLU43_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO27714.1"
                     /translation="MDPAIPVIAMTARTMEEEKEACFKAGMNDHIAKPIEPARLFGTI
                     KQWVLKERLQELGPSRRTQQKAGRFAGEGLSIAGINTESGLQRVSGNQELYHNLIKNF
                     AMNQPETVRQMAEAVQEGRYETIEKTAHMLRGVAGNIGAVTVQKLCESIEKNAAAVKD
                     IREIEQLFEQLGNEMHKVVRSILENTDLDRQAEMNAEIPENPDMKLEELYKLLEDGDS
                     AAVDSFHLLRGLIRKYTDEKLFSLIEKQIDNYQFEEADIQLKKIMHAGKEGNLS"
     gene            1080..2174
                     /locus_tag="PLU43_04575"
     CDS             1080..2174
                     /locus_tag="PLU43_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOO27715.1"
                     /translation="MSDIDKNKPVVLVVDDVPDNITLLSGLLKEYYQVKVALNGEKAI
                     AMVRKSPPDIILLDVMMPVMDGYETCRILKANDAWKDIPVIFLTTKNEVEDESRGFSY
                     GAADYIAKPVNPVILLSRIKTHLTLKQASDFLKNKNQYLEEEISRRTQEVGLIQEVSI
                     MAMAALAETRDNETGYHIQRTKLYVKELCGLLRNDSAYADQMQPALINLIVTSAPLHD
                     IGKVGIPDAILLKPGKLTEEEFEVMKTHTTLGRDAIMKAEQLMNRPETFLLIPKEIAY
                     SHHEKWNGTGYPQGLSGMDIPLSARILALADVYDALISKRVYKPPFPHEKAVGIIRED
                     SGRHFDPEIVRVFLKNSGLFHEIAETYKDI"
     gene            2207..2944
                     /locus_tag="PLU43_04580"
     CDS             2207..2944
                     /locus_tag="PLU43_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOO27716.1"
                     /translation="MKMKTKIILYIAGCLLLGVVIAIFTVVLITLPYIEKEETENIKN
                     DIDRISLLLESEIQAVERTQGDWTWWNDSYDFAENKTASFVEDNLGVATLEQLNLTGM
                     LFYDKDGSNIYTEYVNDDYKTVLQDTFSQKDTIAQLFQNVDEMYMNSGISVIGNRLFL
                     VSAAGITSTDGTAQANGYLVMIKELDKEYMPYMEDLLGVRIAYRSGDTAEHHVFNGIA
                     FDKIIRNGEQLTYYGTADDLNSTCIFY"
BASE COUNT          933 a          538 c          784 g          714 t
ORIGIN      
        1 tgaagcaggc aaaggggctt aacgtagcag ggtatctgac aaagccggtc agccagtctt
       61 ccctgtatga tgcgatcgta agactgatag gttccggcat ggaaaatgat acggttcgca
      121 gttcccgtat ggaaaaggac tatcagctgg caggattgaa agtactgctt gcggaagata
      181 acgaaataaa tcagcaggtt gcggcggaac tgctgtcaag ccagggaatt acagtggata
      241 ttgtctggaa cggcagtcag gaggatggat cctgctattc cggttattgc catgactgca
      301 cggactatgg aagaagaaaa ggaagcatgt tttaaggcgg ggatgaatga tcatatagca
      361 aaaccgatcg aacctgcccg tctgtttggc acaataaagc aatgggtttt aaaagaacgc
      421 ctgcaggaac tgggaccgag ccgccggaca cagcagaaag ccggccgttt tgcaggggaa
      481 gggctttcta ttgctggaat caatacggag agcgggcttc agcgtgtatc gggcaatcag
      541 gagttatatc ataatctgat aaagaatttt gcgatgaatc agccggaaac agtcagacag
      601 atggcagaag cagttcagga aggccgttat gagaccattg aaaagacagc ccatatgctg
      661 cggggagttg ccggcaatat tggcgcagtc acagtacaga agctatgtga atccattgaa
      721 aaaaatgctg cagcagtcaa agacatacgg gaaatagagc agctatttga acagctggga
      781 aatgaaatgc ataaggttgt ccggtcgatt ctggaaaaca cagacctgga caggcaggca
      841 gaaatgaatg ctgaaattcc tgaaaatccg gatatgaagc tggaggaact gtataaactg
      901 ctggaggacg gcgacagtgc tgctgttgac agttttcatt tactgcgggg cctgatcaga
      961 aagtataccg atgaaaaatt gttctctttg atcgaaaagc agatagataa ttatcagttt
     1021 gaagaagcag atatacagct gaaaaagatt atgcatgcag gaaaggaggg gaatctgtca
     1081 tgagcgatat tgataaaaac aaacctgtcg tacttgtggt agatgatgta ccggataata
     1141 ttacattgct gagcgggctg ctgaaagaat actatcaggt caaagtggcc ttgaacggag
     1201 aaaaggcaat tgccatggta aggaaaagtc cgccggacat catattgctg gatgtgatga
     1261 tgccggtcat ggacggttat gaaacatgcc gtattctgaa agcaaacgat gcatggaagg
     1321 atataccggt cattttcctc acaacaaaga atgaagttga agatgagagc cgcgggtttt
     1381 cttacggtgc tgccgattat attgcaaaac cggtcaatcc ggtcattctg ctttcaagga
     1441 tcaaaacgca tctgacctta aagcaggcca gcgattttct gaaaaataaa aatcaatatc
     1501 tggaagaaga gatttccaga aggacgcagg aagttggact gatccaggaa gtatccatta
     1561 tggcaatggc cgctttggca gaaacaagag ataatgaaac cggctatcat atccaacgga
     1621 cgaaactgta tgtaaaggag ctgtgcggtc tgctgagaaa tgactctgca tatgcagacc
     1681 agatgcaacc ggctctgata aatctgatcg taacgtctgc gccgctccat gatatcggaa
     1741 aagtgggaat tccggatgcg attttgttaa aacccggaaa actgacagaa gaggaatttg
     1801 aagtaatgaa aacgcatacg acgcttggaa gggatgcgat catgaaagcg gaacagctga
     1861 tgaacagacc tgaaacgttt cttctgattc caaaggagat cgcgtattcc catcatgaaa
     1921 aatggaacgg gacaggatat ccgcagggac tttcgggtat ggatattccg cttagtgcaa
     1981 ggatccttgc cctagcggat gtctatgatg cgctgatcag taaaagagta tataaaccgc
     2041 cgtttcctca tgaaaaggca gtaggaatta tcagggagga ttccggcagg cattttgatc
     2101 cggaaattgt cagggtgttt ttgaaaaaca gcggactgtt tcatgagatt gcggaaactt
     2161 ataaagatat ctgacgggtc ttgtaacgga aagcaaggaa aataaaatga aaatgaagac
     2221 aaagatcatt ctgtatatcg caggctgtct gctgcttggt gttgtgatcg cgatctttac
     2281 ggtcgtactg attacgctgc cttatataga aaaagaagaa acagagaata tcaaaaatga
     2341 tattgacagg atttcgcttt tgctggaatc tgagatacag gctgtggaaa gaacgcaggg
     2401 agactggaca tggtggaatg attcctatga tttcgcggaa aataaaactg cgtcctttgt
     2461 ggaggataac ctcggcgtgg cgacacttga acagctgaat ctgacaggca tgctttttta
     2521 tgataaggat ggctccaata tttatacgga atatgtgaat gatgattata aaactgttct
     2581 gcaggataca ttttcacaga aagatacgat cgcgcaattg tttcaaaatg tcgatgaaat
     2641 gtatatgaat tccggaatat ctgtcatcgg aaatcgactt tttctggtat ctgctgcggg
     2701 aattacttct acggacggta cggctcaggc taacggatat ctggtcatga tcaaagaact
     2761 ggataaggag tatatgccgt atatggaaga cctgcttggg gtccggatcg catatcgttc
     2821 cggggatacg gcggaacatc atgtattcaa cgggattgcc tttgataaaa taatcaggaa
     2881 tggagaacag ctcacatatt acggaacagc ggatgaccta aattccacgt gcatttttta
     2941 ttagcttaaa agaacactat ttcaagaag
//
LOCUS       DAOSYE010000207         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24689_length_2968_cov_3.074837, whole genome shotgun sequence.
ACCESSION   DAOSYE010000207 DAOSYE010000000
VERSION     DAOSYE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24689_length_2968_cov_3.074837"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLU43_04585"
     CDS             <1..873
                     /locus_tag="PLU43_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO27717.1"
                     /translation="QVSLRIAQILSEKAFSFTPNEYLSIHKKLFAGIYGYAGKLRDYN
                     ITKKEWVLDGATVMYGSASELKATLDYDFSQEKNYSYKGLGMEETIHHLALFVSGLWQ
                     IHVFGEGNTRTTAVFFIKYLKTLGFNVTNDIFAENAWYFRNALVRANYNDLKNDIHET
                     TEYLELFLRNLMLNEKNELHNRAIHIRGIFTDYKKPDIGREKPDIGREKPDIQMLDLY
                     QEKMQELSLIVQTQISKLYDAFGMEQIFGRSDVEKVLDLKSSRTSELLKELQEAGIIV
                     PVKGYGKGKYSFLK"
     gene            1007..1624
                     /locus_tag="PLU43_04590"
     CDS             1007..1624
                     /locus_tag="PLU43_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO27718.1"
                     /translation="MTVVKNAEERKEEILDAAERLFGVKGYDKTSVLDIMNEVGIAKG
                     TLYYHFRSKEEILDAMTERITRRVTAHAKKIAQDPSGPVCERLFRVLLSLNLRESGKE
                     ELLTLIHRPQNALLHQKSRQQLVKEITPVLTELLLEGIREGLFDTEYPKESIEMTLIY
                     VQEVFDDTQEANEEEMQDRVSAFIVNLERIFGAPAGCFAFVRQLV"
     gene            1636..2955
                     /locus_tag="PLU43_04595"
     CDS             1636..2955
                     /locus_tag="PLU43_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO27719.1"
                     /translation="MKKFMILWAGEMISSIGSGLTAFGLGVYVYRQTGLASATALVSL
                     LALLPAVLLGPVAGVLADRYDRRLLMVAGDSLSALGLLYILVSLINGRAELLQICIGV
                     TVSSVFASLLEPSYRATITDLLPEEHYSKASGLVQLASSAKYLISPALAGLLMKFSGI
                     RVILLIDICTFFITVQATLAVKKRIAAKEKKCRGRFMEQLREGFLIITKKRGIFLVIL
                     LSAGICFFLTFIQTLSTPMILAFSDEATLGICETICACGMLVSSLILGIVSIKKGYVK
                     MLSLSMFFAGLFMMLFSLRENMIIVGIGGFLFFMAIPFANTGLDILIRTNIPNELQGR
                     AWGTISLISQLGYVLANACIGVLADYLFEPAMRKGGILAGSIGKLIGTGTGRGTALLI
                     MTGGLALCIIAVLIYRTKPIRELEKEHPVRICRNINEKIYIKKHFSV"
BASE COUNT          862 a          544 c          768 g          794 t
ORIGIN      
        1 caggtgtcgc ttcgcattgc gcagatctta tctgaaaaag catttagttt cacaccgaat
       61 gaataccttt ctattcataa aaaattgttt gctggaatat atggttatgc cggaaagctg
      121 cgtgattata atattacaaa gaaggaatgg gtgcttgatg gtgcaaccgt aatgtatgga
      181 agtgcatcag aactaaaagc aacattggat tatgattttt cacaggagaa aaattatagt
      241 tacaaggggc ttggcatgga agaaacaatc catcatttgg cgctctttgt atctggatta
      301 tggcagattc atgtttttgg tgaaggcaat acgagaacta cagcagtgtt ttttattaag
      361 tatctgaaaa cacttggttt taacgttacg aatgatattt ttgccgagaa tgcctggtat
      421 ttcaggaatg cattagtccg tgcaaattat aatgatttga aaaatgatat tcatgagaca
      481 acggaatatt tagaattatt ccttagaaat cttatgctga atgaaaaaaa tgagctccat
      541 aatcgggcaa tacatataag aggaatattt actgattata aaaaaccgga cattggcaga
      601 gaaaaaccgg acattggcag agaaaaaccg gacattcaga tgttggattt gtatcaggaa
      661 aagatgcagg agttatcatt aattgtacaa actcagatct caaaattata tgatgctttt
      721 ggaatggaac agattttcgg tagaagtgat gtggaaaaag tacttgattt gaaatcgtca
      781 aggacgtcgg aacttcttaa agaattgcag gaagccggaa tcatagtccc ggtcaagggg
      841 tacggaaaag gaaagtattc ctttttaaaa tgagtgaata aatgaattac tcagacttga
      901 caaatacagt tgcggtttgt ataatccatt tatagaccga cggtcggtct ataaaaacaa
      961 agagcaaaag taaaacgggt ttcagaaaaa taaagaaggt gaagagatga cagtcgtaaa
     1021 aaatgcggag gaacgcaaag aagagatcct ggatgcggct gagaggttgt ttggtgtaaa
     1081 gggatacgat aaaacaagcg tacttgacat tatgaacgaa gtcgggatcg caaaaggaac
     1141 actgtattat cattttaggt ctaaagaaga gatcctggat gcgatgacag aacggatcac
     1201 aaggcgcgtg acggcgcatg caaaaaaaat cgcgcaggat ccttccggac cagtatgcga
     1261 aaggctgttt cgtgtgctgc tttcactgaa tcttagggaa agcggaaaag aagagctgct
     1321 gacgctgatc cacaggccgc agaatgcact gctgcatcag aaatcaaggc agcagcttgt
     1381 aaaggagatc acgcccgtgc ttaccgaact gcttctggaa ggaatacggg aaggtttgtt
     1441 tgatacggag tatccgaagg aaagcatcga aatgacgctg atctatgtac aggaagtatt
     1501 tgatgataca caggaagcaa acgaagaaga aatgcaggac cgggtcagtg catttattgt
     1561 aaatctggag agaatctttg gggcacctgc cggctgcttt gcgtttgtca gacagcttgt
     1621 ataggagaga gaactatgaa aaaatttatg atcttatggg caggcgagat gatctcgtcg
     1681 atcgggagcg gcctgacggc attcggactt ggtgtttatg tgtacagaca gaccggtctg
     1741 gcttctgcca cggcacttgt ttccctgctg gcactgcttc ctgcggtgct gctcgggccg
     1801 gtggccggtg tactggcgga cagatatgat agacggctgc tgatggttgc gggtgacagc
     1861 ctttctgcac ttggactgct ctatatactg gtttctctga taaacggaag ggcagaactg
     1921 ctgcagatct gcatcggtgt tacggtcagt tccgtgtttg catcccttct ggagccgtcc
     1981 tacagggcaa ccatcacgga tctgcttcct gaagaacact attcgaaagc aagcggactt
     2041 gtgcagctgg ccagttcggc gaagtatttg atttctccgg ctttagcggg acttcttatg
     2101 aagttttccg gcatccgggt gatcctgctg atcgatatct gtacattttt tattaccgta
     2161 caggcgacat tagccgtaaa gaagagaatt gcggcaaagg aaaagaaatg ccggggacga
     2221 tttatggaac agctgcggga aggatttttg atcatcacaa aaaaaagagg aatttttctg
     2281 gtgattttac tgtcggccgg catctgcttt ttcctgacgt ttattcagac gctgtccact
     2341 ccgatgatac tggccttttc agatgaagcg acacttggca tctgtgagac gatctgcgcc
     2401 tgcgggatgc ttgtgtcaag tctgatcctt ggaatcgttt ctattaagaa aggttacgtt
     2461 aaaatgctgt cgttgtccat gttttttgcg ggactgttta tgatgctgtt tagcctgcgt
     2521 gaaaatatga tcattgtcgg tatcggcgga tttctgtttt ttatggcgat cccgtttgca
     2581 aataccggtc ttgatattct gatccgtaca aatattccga atgaactgca gggacgtgcg
     2641 tggggcacga tcagcctgat ctcccagctg gggtatgtgc tggccaatgc ctgtatcggc
     2701 gtactggccg attatctgtt tgagcctgcc atgagaaaag gcgggatact ggccggaagc
     2761 atcggaaaat tgatcgggac cgggaccggc agaggtacgg ccctgctgat catgaccggc
     2821 ggcctggctt tgtgcataat agcagtcctg atctatcgga caaagccgat ccgtgaactg
     2881 gaaaaggaac atccggtcag gatatgccgg aatataaatg aaaaaatata tataaaaaaa
     2941 cacttttccg tctgatgaaa atcatgta
//
LOCUS       DAOSYE010000208         2966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24718_length_2966_cov_2.889042, whole genome shotgun sequence.
ACCESSION   DAOSYE010000208 DAOSYE010000000
VERSION     DAOSYE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24718_length_2966_cov_2.889042"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLU43_04600"
     CDS             complement(<1..228)
                     /locus_tag="PLU43_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOO27720.1"
                     /translation="MEQEITVQKTKESSQTALCIMAGNLLISAVFLASGLLGKSIAAV
                     SEGIDSLTDAVSSLLLLLGFKIASREPDSLHP"
     gene            complement(255..698)
                     /locus_tag="PLU43_04605"
     CDS             complement(255..698)
                     /locus_tag="PLU43_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOO27721.1"
                     /translation="MIYRYHKNRSSSFNPHRGQGRIMSLLKMQPQISQKDLSFLLNMS
                     KQALAELLGKLEAAGYITRTPSEEDRRVMIIRLTEAGQAAADAMAKQEKESDSIFSCL
                     TADEQNTLRGYLKRMIEKSEKEMPENWRRHCKNSHRRFGQNNYWN"
     gene            1036..1272
                     /locus_tag="PLU43_04610"
     CDS             1036..1272
                     /locus_tag="PLU43_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease of phosphate ABC transporter"
                     /protein_id="HOO27722.1"
                     /translation="MKKLFAVADQYLKSCGWKDMALIKLCLLAMGMLIGMAIPGKSRK
                     AVVLGAVVAFIATYIPLMYKFLPILREGLSGDET"
     gene            1374..2060
                     /locus_tag="PLU43_04615"
     CDS             1374..2060
                     /locus_tag="PLU43_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOO27723.1"
                     /translation="MKIFSKDTLYFLQEVHQQNSKEWFGDNKDWYNEALITPLKNFVL
                     FLSETMSGIDGEFELMPGINKTISRINRDTRFSKDKSLYKDRMWVTFKKAGKDKTDYP
                     AYFFEISPYSFRYGMGFFSASSGSMKSLRALMQKKEKQFGKIVSAIEKKGIFQVEGEI
                     YKKNMFCDRPSELQKWYNRKNIYLVYNGENVGELFQEDFMEHIKEDFISLTDLYLFFV
                     EAMTESTQGI"
     gene            complement(2173..2844)
                     /locus_tag="PLU43_04620"
     CDS             complement(2173..2844)
                     /locus_tag="PLU43_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HOO27724.1"
                     /translation="MKNENPNTIFHPIDFDHWDRKQYFYYFTKMLPTGFSISVETDIT
                     NTYHMIKKQNKKFFPAYLYLASKLISEQQEFRIASQNGQLGYYEVLHPSYACFHQDDK
                     TMSHMWTAYAPDFGTFYQNYTEDQNNFADHHGIQAKPGMPPQNLCMIGMLPWVSFTSY
                     APVPFAAGSCYFPVLQAGKFFDKEGKKMMPLSITVHHAVADGYHVSLFLEKFQTCMAH
                     PERWA"
BASE COUNT          896 a          511 c          646 g          913 t
ORIGIN      
        1 tggatgcagg ctgtcgggtt ctctggacgc aatcttgaaa ccgagaagca gaagcagtga
       61 actgactgcg tctgtaagac tgtcaattcc ttcagaaaca gcggcaatgc tttttccaag
      121 cagaccgctt gctaagaata cggcagatat cagaagattt cctgccatta tacagagtgc
      181 cgtctgactg ctttcttttg ttttttgtac ggttatttcc tgttccataa ataaaacctc
      241 cccgagtaat aaatttagtt ccaatagtta ttttgaccga agcgcctgtg agagtttttg
      301 cagtgacggc gccagttttc cggcatttct ttttcacttt tttcgatcat gcgcttcaaa
      361 taacccctca gtgtattctg ttcgtcggct gtaagacatg aaaagataga atctgattcc
      421 ttttcctgct ttgccattgc gtccgcagct gcctgtcctg cttccgttaa gcgaataatc
      481 attacacgcc tgtcttcttc cgaaggagtt cttgtaatat agccggcagc ttccagtttg
      541 ccaaggagtt ctgctaaagc ctgcttactc atattgagaa ggaagcttaa atctttctgg
      601 ctgatctgag gctgcatttt taataatgac ataattcgcc cctgtccgcg atgcggattg
      661 aaggaagaag aacggttttt atgatatcga tatatcattt catgccgcat aagagcctct
      721 aactgcaaca ctaattccat taattcctgt tcgttggatt ccattgataa tacctcctga
      781 tttttaatgg tcatgtactt gacaaattaa taataaagta cttgacctaa attgtcaagt
      841 gctttacgat tttttttagc agaaagttta tggttaaaaa aatatcgata aatagtataa
      901 gaattgcagt ggaaaatcag aacgatgctt tcgtaatata aacggattca gattgcactt
      961 tgccaataaa tgtattatga tttatataag aagagtatcc tgtaaataaa gaaaaatgta
     1021 aggaaggtta tgtgtatgaa gaaattattt gctgttgcag accagtacct gaaaagctgt
     1081 ggctggaaag atatggcact gatcaagctc tgcctgcttg cgatgggaat gctgattggt
     1141 atggctatac cgggaaagtc cagaaaggca gttgttttgg gagcagtcgt agcatttatt
     1201 gcgacttata ttccgttgat gtacaaattc cttccaatat tacgtgaggg gttatcgggg
     1261 gatgaaacgt aattgatgct aagtaagagc gaccgcatta cggccgcttt tttcttaaaa
     1321 aaatgagagg aaatgtgata tttctgtttt agtaatatca taatagaaga aaaatgaaaa
     1381 ttttcagtaa ggatacattg tattttttac aagaggtaca tcagcaaaac agtaaagaat
     1441 ggtttggaga taataaagac tggtataatg aggcgttgat aacaccgttg aaaaattttg
     1501 tgttgtttct aagtgaaaca atgtccggta ttgacggaga atttgaactg atgccgggaa
     1561 taaataaaac gatatccagg attaaccggg ataccagatt ttcaaaggat aaaagcttgt
     1621 ataaagacag aatgtgggtg acatttaaaa aagcaggaaa agataaaaca gattatccgg
     1681 catacttttt tgaaatcagt ccctacagtt tccgttacgg aatgggattt ttcagcgcat
     1741 cttccggctc tatgaaatca ttaagggctc tgatgcaaaa gaaagagaag cagtttggga
     1801 agatagtcag cgcaatagag aaaaagggga tcttccaggt tgaaggtgag atctataaga
     1861 aaaatatgtt ttgcgacaga ccgtctgaac ttcagaaatg gtataaccgt aaaaatattt
     1921 atttagtgta taatggtgag aacgttggag aattgtttca ggaagatttc atggaacata
     1981 taaaagaaga ttttatttcc ttaactgatt tatatttatt ttttgtggaa gccatgacag
     2041 agagcacaca gggaatataa aaagccatta cggaaaagcg gtcaaatggt cagaaactcg
     2101 gcccgcatcg ttttttccag tattttggtg agacagatgt aaatacggca tacgcaaggg
     2161 aatgtgaata tgttatgccc acctttcagg gtgggccatg catgtctgaa acttttctaa
     2221 aaataaactc acatgatatc catccgccac agcatgatga acggtaatgg acaacggcat
     2281 cattttcttt ccttctttat caaaaaattt tccggcctgc aatacgggga aatagcagct
     2341 gccggcggca aacggaaccg gggcatagct tgtaaacgaa acccatggca gcatgccgat
     2401 catacagaga ttttgcggcg gcatcccagg ttttgcctgg atgccgtgat ggtctgcaaa
     2461 attattttga tcttctgtgt aattttgata gaatgtgcca aagtccggcg catatgcagt
     2521 ccacatatga gacatcgtct tgtcatcctg atggaaacag gcataggatg gatgtaagac
     2581 ctcataatat cccagctgtc cgttttggct ggctatacgg aattcctgct gttccgatat
     2641 cagctttgat gctaagtata agtaagcagg aaaaaatttt ttgttttgtt tttttatcat
     2701 atggtaggta ttggtaatat ctgtttccac actgatggaa aatccggtag gcagcatttt
     2761 ggtaaaataa taaaagtatt gttttctatc ccaatgatcg aaatcaattg ggtgaaagat
     2821 agtgtttggg ttttcatttt tcatatgatt ttctcctttt tctcttgcat ttcagtaatt
     2881 gtaaatccac agccgcatgt tttcaaggaa cgttccgtag agtttaagct gaattaaaat
     2941 gggaaggtat tttacaacta ggtgga
//
LOCUS       DAOSYE010000209         2962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24765_length_2962_cov_3.001032, whole genome shotgun sequence.
ACCESSION   DAOSYE010000209 DAOSYE010000000
VERSION     DAOSYE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24765_length_2962_cov_3.001032"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..885)
                     /locus_tag="PLU43_04625"
     CDS             complement(52..885)
                     /locus_tag="PLU43_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27725.1"
                     /translation="MTTEEREIAELQNNLEMLRKLFGWTAEQLGNRLGVTKQTVLNLE
                     HGKPVMTKIQYIAIRSVMETEAMSREGEEKENLLKVLSLVFNEDKTITEEQREKALDA
                     AKVVSTATAAGAGVTAMGVLTGSLTALGIGAVAVNPAIVAVAGAVGVGGWVAGMLIRQ
                     SKLRNRIRKGQRKMINLGQIVKTAEPLLKQAGRFIKANPHLLIEGGLAGGLLVSLGYN
                     AYAENEHEQRDKLVENQLRKDQAIIKDLSVKAQKVDKLEKCNELLMDALTKKSPSTVG
                     A"
     gene            complement(1354..2052)
                     /locus_tag="PLU43_04630"
     CDS             complement(1354..2052)
                     /locus_tag="PLU43_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO27726.1"
                     /translation="MLIRKRKKLIAYKAVVFDMDGTLYYQNRLRLLMAARLFFYYVCH
                     PLKIKELLAVKEFRSVREHWDQIKEKSPEAHLLDEAQYAYVAAKIKLEADEVRSAVLK
                     WIYKEPLSALWKSRDRKIAELIERLQAKGSRILVFSDYPAREKLEALGILADGIYSAS
                     DERLLELKPSPKGLRLIMEDYDLKPEELLLIGDRFSRDGMAAVNAGTDYLILKKTFAG
                     RRQIYAKLFEEINA"
     gene            complement(2056..>2962)
                     /locus_tag="PLU43_04635"
     CDS             complement(2056..>2962)
                     /locus_tag="PLU43_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HOO27727.1"
                     /translation="ARPDHWVKNAFILPGVVIAVLLADPQITAETWIKLVIGFFATCF
                     IASANYVINEWLDAEFDQYHPTKKFRPVVSEDMKFSLVMAEYFLFIVLGVVCSLFVNR
                     LFLYMELWLLVMGVIYNVKPIRTKDIPYLDVLSESINNMIRLLLGWFIITSEYQPPSS
                     ILVGYWFAGAFLMVTKRFAEYRMIGDPQKAGLYRKSFKYYTENSLLVSAFFYAMCATF
                     FIGIFMIKYRIEYLVAMPFVFGLFCFYLWISFKPDSAVQKPEKLYREKKLLVYIVLLI
                     IILLILTFVDIPLLNYWVDIRLIGV"
BASE COUNT          838 a          735 c          503 g          886 t
ORIGIN      
        1 ttaaaaacca tatccttctt ctttgtcaca tattcaccca tgagatacct cctatgcccc
       61 aaccgtgctt ggggactttt ttgtcagagc atccattaga agctcattac atttctcaag
      121 cttatcaacc ttctgcgctt ttacgctgag atcttttatt attgcctggt ctttccgaag
      181 ttgattttca acaagcttat ctcgttgctc atgttcgttt tctgcataag cattgtatcc
      241 aagtgaaacc aaaagaccac cagcaaggcc tccctctatc aaaaggtgcg gattggcttt
      301 tataaacctt cccgcctgtt tcaaaagagg ttcagcagtt ttaacaatct gacccaaatt
      361 aatcatcttt ctttgcccct ttcttattct gtttctcagc ttgctttgtc ttattaacat
      421 acctgcaacc cagcctccaa caccaacagc tcctgctacc gcaactattg caggatttac
      481 agcaacagct cctattccaa gagctgtgag ggagccagta agcacaccca tagctgttac
      541 tccagctcca gcagctgttg cagttgaaac aaccttagct gcatccaacg ctttttctcg
      601 ctgttcctca gtgattgttt tatcttcatt aaagaccaag cttagaactt tcagaagatt
      661 ctccttttct tcaccttctc gactcatagc ttcagtctcc atcactgatc tgattgctat
      721 atattgaatc ttagtcatca caggcttacc atgctcaaga tttagaactg tctgctttgt
      781 cacgccaagt ctattaccaa gttgctctgc agtccatccg aaaagctttc tgagcatttc
      841 aagattattc tgtagttcag ctatttctct ttcctcagta gtcatagata tgtacctccg
      901 tggttataat atagcaaata tttatttgtt agtcaatatt ttggactgat atttttatat
      961 ttaactaatc tcgtgatttt tacctgaata taaaaaagaa cagatgccat catatgcatc
     1021 tgctcttata ctatgtgatt atatctgcta acctgggtct caatatagct tgttacaaat
     1081 gaaataatgt tgatattttt tcttggtctc ccgaatatcg agtaccatac cgagtaccac
     1141 gggagtacca tttttctata aattataata atttataaca agttacatac gagaacaaat
     1201 taccacgaac ccagtattta tgcggtttca tagagtttta tgactcttta tggagattta
     1261 tgaaacaggc gttcatataa cgataccaag tttcatctga attactgtcc tttccggttc
     1321 tatcttacta ttatattgct gatcactgcc atttcaggca ttgatctcct caaacagctt
     1381 tgcatatatc tgcctgcgcc ctgcaaatgt ctttttcaga atcagataat ctgttccggc
     1441 attgacagcc gccataccgt ccctgctgaa acgatcccct atcagcagca gttcctccgg
     1501 cttcagatca taatcttcca tgatcagccg cagccctttg ggcgacggtt ttaactccag
     1561 cagacgttca tccgaagcac tgtatatacc gtctgccagg atcccaagtg cctccagttt
     1621 ttccctggca ggataatcgg aaaatacaag gatcctgctc cccttggctt gcaggcgctc
     1681 aatcagttct gcaatttttc tgtccctgct tttccacagt gcggacagcg gctctttata
     1741 gatccacttc aaaacagcag accgcacctc atccgcctca agctttatct ttgcagccac
     1801 atacgcatac tgtgcctcat caagcagatg tgcctcgggg cttttctctt ttatctgatc
     1861 ccagtgctcc cttacggaac gaaattcttt tacggctaaa agctccttta ttttcagcgg
     1921 atggcaaaca taatagaaaa aaagcctggc cgccatgagc agccggagcc tgttctgata
     1981 atacagtgtt ccgtccatat caaatacgac cgctttatac gcaataagct tttttctttt
     2041 cctgatcagc accggctata ctcctatcag acggatatcc acccagtaat tcagcagcgg
     2101 aatatctaca aatgtcaaaa tcaataaaat aatgatcagc agcacgatat aaaccagcag
     2161 ttttttttca cgataaagct tttccggctt ctgtacggca gaatccggtt taaagctgat
     2221 ccataaataa aaacagaaca atccgaacac aaacggcatc gcaaccaaat attcgatccg
     2281 gtacttgatc ataaaaatac cgataaagaa ggttgcgcac attgcataga aaaacgccga
     2341 cacaagcagg gaattttccg tataatactt gaacgatttt ctatacagac ccgctttttg
     2401 cggatctccg atcatacggt attctgcaaa acgctttgtt accatcaaaa atgcgccggc
     2461 aaaccagtag cctaccaata tactgcttgg cggctgatat tcgctcgtaa taataaacca
     2521 gccgagcaga agcctgatca tattgttaat ggattcggaa agaacgtcca gatatggaat
     2581 atcctttgtg cggatcggtt tgacattata gatcacaccc atcacaagca gccagagttc
     2641 catataaaga aacagcctgt tgacaaacag ggaacagact acacccaaaa cgataaaaag
     2701 aaaatattcc gccattacaa gggagaattt catatcctcc gatacgaccg gacgaaattt
     2761 tttggtcgga tgatactgat caaattccgc atccagccat tcattgatca cataattggc
     2821 agacgctata aaacaagtgg caaaaaagcc gattaccagt ttgatccatg tctctgccgt
     2881 tatttgggga tccgccagta aaaccgcaat gacaacacca ggaagaataa acgcgttttt
     2941 cacccaatga tcaggtcttg ct
//
LOCUS       DAOSYE010000210         2961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24770_length_2961_cov_3.667928, whole genome shotgun sequence.
ACCESSION   DAOSYE010000210 DAOSYE010000000
VERSION     DAOSYE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24770_length_2961_cov_3.667928"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..517)
                     /locus_tag="PLU43_04640"
     CDS             complement(245..517)
                     /locus_tag="PLU43_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOO27728.1"
                     /translation="MSEMITNNGEQRLHDTPDEHPNEKKVNKYCYILLAVFLGDFGIH
                     QFYAGRVRRGILYLIFCWTFIPELLSIFDIIKACGKIKDTEDQIWM"
     gene            complement(534..917)
                     /locus_tag="PLU43_04645"
     CDS             complement(534..917)
                     /locus_tag="PLU43_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27729.1"
                     /translation="MGFTELLGVKIEKLKLNGNYSMEELYEKIKSVSFEAGTPSLVKN
                     GFAWVIVFPQLDRNNQVQILGTKGNYSVQRAVQPAGVDKMAANMALDKLTGGLSGMSG
                     AFGDTKKKCMELVVKTAETINALGI"
     gene            complement(1035..>2961)
                     /locus_tag="PLU43_04650"
     CDS             complement(1035..>2961)
                     /locus_tag="PLU43_04650"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOO27730.1"
                     /translation="YFYERVGCLSEGNPLFLRIAAIEMNRFFENGKTEKQRIESEKEA
                     IEQARRNLWDYLENRVYEQWNKEMQDFILEISVVDEFDEELAQQITGRSNVKQLIEEV
                     KETTGFLQEKNQVYTCRKTVLICMRRQLGCRLTSEQIRQLYIRAGTCYEKRGDMAGAL
                     KMYETAQGKEQISSLLVENAKQNPGSGCYFELRRYYLSLPDEMICKSPLLITGMSMLQ
                     SMLMNIEESERWYQQLQLFAEKHKGTLRHEAKSRLIYLDIALPHRGIARMTDLLLQAG
                     KLLCEKKVILPEFSVTSNLPSMMNGGKDFCEWSLHDKELAGSIGKVVELVLGRCGNGL
                     VNLALAESLLEKGGEDFEITLLLQRGRMQAEAGGKTEQCFVAVGLLSKQRVMHNHLAE
                     AVEQLDSFKKRAIQDAPQLLHNIETLRCRYFLYLGKTEEVKQWAGTAPEEEKEFCTLE
                     RYRYLMKARIYIQDEKYDCAVGLLEKLLYYADVMHRTYVEIEAGLLLSMALYRMNNAI
                     WKEYFQKSFLKAKKYHFVWIISQEGIAVLPLLKRTIGTSQEEYEKTVLEQTVKMAQYY
                     PLYLEKESFFDEELTAAELQILKLHGRGMSTREIQKLMSISESTLKFHNSNIYRKLDV
                     KNKNEAVITAQKIGLLL"
BASE COUNT          768 a          751 c          468 g          974 t
ORIGIN      
        1 attttcttct ccggtccatg catcgcgtat gaccatggac accccccaga tcccaaaacc
       61 tgctgctgcc acgatcataa gcacaaaaac acctgctata cctcctaaaa ttgcttttat
      121 tgttctattc atatttttct tcccccggtc agtctctctt tccgaaaaat aaataagcag
      181 ggatccactg tcgaattcct acttatttat tataatgcat gttttattat attgcaccaa
      241 aaaattacat ccatatttgg tcttcagtat cctttatttt tccacaggcc tttataatat
      301 caaaaattga caaaagctcc ggaataaatg tccagcagaa aatcaaatac agaatccccc
      361 ggcgtacacg tcctgcataa aactggtgaa tcccgaaatc gcccaaaaag acagcaagca
      421 gaatataaca atatttattc acctttttct cattcggatg ctcatccgga gtatcatgaa
      481 gtctctgctc gccattgttt gtgatcattt cagacatctt tagcctcctg ttcctatata
      541 ccaagagcat tgatcgtttc agctgttttt actaccagtt ccatgcattt ctttttcgta
      601 tcaccaaatg ctccggacat accactgagt ccgccggtca gcttatcaag ggccatattg
      661 gcagccattt tatcaacacc tgccggctgt acggccctct gaacagaata attccccttt
      721 gttccaagaa tctggacctg attgtttctg tcgagctgcg gaaatacaat cacccaggca
      781 aatccatttt ttacaagcga cggtgtaccg gcttcaaatg aaaccgattt aattttttca
      841 taaagctctt ccatactata atttccattt agtttcagtt tttcaatttt tacaccaagt
      901 aattctgtaa atcccatgtc acattctcct cataatttat ttgatatcct gaatttttta
      961 tcaaaacaaa ttatggcata ctcctatatt tttgtaacct gtccgaaagt ataggatttc
     1021 agcctgcaaa gcttttaaag taacagaccg attttttgtg ccgtaataac agcttcattt
     1081 ttattcttaa catccagttt tcgataaata ttagaattat gaaatttcag ggtactttca
     1141 gaaattgaca tcaatttctg aatttccctc gttgacatcc ctctcccgtg cagctttaaa
     1201 atctgcagct ctgctgccgt cagttcttca tcaaaaaatg actctttttc aagatacagc
     1261 ggataatact gtgccatttt tacagtctgt tccaacacag ttttttcata ttcctcctgg
     1321 ctcgtaccga ttgttctttt aaggagcgga agtactgcga tgccttcctg tgaaatgatc
     1381 cagacaaaat gatatttctt tgcttttaaa aagctctttt gaaaatactc tttccatatg
     1441 gcattattca ttctgtacaa agccatggaa agcagaagcc cggcttctat ctccacatag
     1501 gttctgtgca taacatccgc ataatacaac agcttttcca gcaatccaac tgcacagtcg
     1561 tatttttcat cctgtatata aatgcgggct ttcatcagat accggtaccg ttccagtgta
     1621 caaaattctt tttcctcttc cggggcagtc cctgcccatt gtttaacttc ctctgtcttt
     1681 ccaagataca aaaaataccg gcaccgcagt gtctcaatat tatgcagaag ctgcggtgca
     1741 tcctgtattg cacgtttctt aaaactgtcc agctgttcaa ctgcctcggc aagatgattg
     1801 tgcatgaccc gctgtttgct cagcaggcca actgccacaa aacactgttc tgtctttccg
     1861 cccgcttctg cctgcattct tcctctttgc agcagtaatg tgatctcaaa gtcttctcct
     1921 cccttttcca gaagactttc tgccaaagca agatttacaa gtccatttcc gcagcggccc
     1981 agcacaagtt ccacaacctt cccgatactt ccggcaagtt ccttatcgtg aagactccat
     2041 tcgcaaaaat cttttccgcc attcatcatt gacggcagat tgcttgttac ggaaaactcc
     2101 ggcaatatta cctttttttc gcaaagcaat ttccctgcct gcaagagcag atcagtcatg
     2161 cgtgcaatac cacggtgcgg cagcgcaata tcaagataaa tcagtctgct tttagcttca
     2221 tgcctgagtg ttcctttatg tttctctgca aatagctgca gctgctgata ccagcgttca
     2281 ctttcttcta tgttcataag catggattgc agcatgctca tacccgtaat cagcaatgga
     2341 cttttgcaga tcatttcgtc tggcaaagat agataatatc tgcgcagctc aaaataacat
     2401 ccgcttcccg ggttttgttt tgcattctcc acaagcagac tggaaatctg ttctttcccc
     2461 tgtgctgttt cgtacatttt cagggcccct gccatatcgc ctcgtttttc atagcatgtg
     2521 ccggctctga tatacagctg tctgatctgt tcagatgtca gtctgcagcc caactgtctt
     2581 cgcatacaaa taagaaccgt ctttctgcat gtatagacct gatttttttc ctgtaaaaaa
     2641 ccggttgtct cttttacctc ttcaatcagc tgttttacat tcgaacggcc tgtgatctgc
     2701 tgtgccagct cttcgtcaaa ttcatctaca acggatattt ccagaataaa atcctgcatt
     2761 tccttattcc actgctcata aacacgattc tcaagataat cccagagatt tcttcttgcc
     2821 tgttctattg cttctttctc tgactcaatt ctttgttttt ctgtttttcc attttcgaaa
     2881 aagcggttca tttctattgc cgcaattctt aaaaacagtg ggtttccttc tgaaagacag
     2941 cccactctct cataaaaata t
//
LOCUS       DAOSYE010000211         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24806_length_2958_cov_3.046848, whole genome shotgun sequence.
ACCESSION   DAOSYE010000211 DAOSYE010000000
VERSION     DAOSYE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24806_length_2958_cov_3.046848"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLU43_04655"
     CDS             complement(<1..614)
                     /locus_tag="PLU43_04655"
                     /inference="COORDINATES: protein motif:HMM:NF028185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOO27731.1"
                     /translation="MIYHGKTCYILETKHTSYAFLVMGSKHLEHLYYGEKLMIPEACD
                     IDELCQPLHEKREFAAGNAVVYAEEWPALSLEDTCLEFSAYGKGDIREPFIEIEFADG
                     SVSNDFLFESASVHNGKEGLAGLPSAYAQEETVQQLTVVLRERCHAVKLELLYSVFPE
                     TDVIVRSARVTNEGKDPVTVKRLMSTQIDFDENGLVFTGFTGAW"
     gene            complement(611..1978)
                     /locus_tag="PLU43_04660"
     CDS             complement(611..1978)
                     /locus_tag="PLU43_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOO27732.1"
                     /translation="MNGNIRKVKVSYGIGAVGKDMVYSLVTGFLLYYYNTILGISATF
                     IGILFMAARIFDALNDPFMGVVVEKTTTKLGKFRPWLLIGTLSNAVVLYFVFAVPKGL
                     EGTGLLVYISVLYLLWGITYTMMDIPYWSMIPAITQSGKDRENISVIARSCAGFGYAI
                     PVALTLALAPVLGSGDERKGFEIFALLVALFFVAAIVVTVINVKEDVRIKKKSVRMKE
                     MFSSLLHNDQALIVVVTIIVFNASLYLTQQLAIYFFKYKIGQDSLYGIFGTVGGAAQI
                     LSMMSLPALRKKWECKKILKGAIITALSGYMMLFILGTLNITNLILLCIAAIIIFCGF
                     GLATVLTTIFLADTVDYGEWKNHARNESVVFSLQTFVVKLASAFSVLIAGVGIDLIRL
                     DKTAVTQTAGTLLGLRLIMLVIPMIGLILSISFFTNKYKLNETMLQKITGELKERHDK
                     ERDRK"
     gene            complement(1998..>2958)
                     /locus_tag="PLU43_04665"
     CDS             complement(1998..>2958)
                     /locus_tag="PLU43_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOO27733.1"
                     /translation="IAGGFGWCMFDYNTHQDFGSGDLICYHGVLDMFRNPKLAAAVYA
                     SQGTRQNILEISSNMSIGEYPSGNIGTVYAFTNADCVRLYKNDQFVKAFYPDRKHFPH
                     MPHPPVAIDDFIGELMEKGEGYDTKTAAAVTEVLFAVAKFGQNNLPFKYKIKMMKVIM
                     RGRLKLEDGLRLYYKYVGNWGGKVTSYRFEAIRNGAVVKTVIKEPAEQLVLEAKVSSH
                     TLYDKGTCDAAAVRLRMLDQNGNLLSYYQEPLRLSTEGPLRLLGPSVISLKGGMGGVY
                     VRTTGTEGTGSLTIEGEQIQPKTLQFEVITTGPNDSGLCKIKK"
BASE COUNT          802 a          787 c          543 g          826 t
ORIGIN      
        1 ctccaggctc ctgtaaagcc ggtgaataca agaccattct catcaaaatc tatctgtgtg
       61 ctcataagtc tttttaccgt caccggatct tttccttcat tagttaccct tgcactccgc
      121 acgatcacat cagtttccgg gaaaacggaa tacagcagtt ccaatttgac cgcatggcag
      181 cgttcccgca gcacaacggt cagctgctgc acggtttctt cctgtgcata agcagaaggc
      241 agtccggcaa ggccctcttt cccgttatga acagacgccg attcgaacag aaaatcattg
      301 ctgacgctcc cgtccgcaaa ctcaatctca ataaacggct ctctgatatc accttttcca
      361 taagcggaaa attccagaca ggtatcctct aaactcaaag ccggccattc ctccgcatat
      421 acgaccgcat ttccggctgc gaattctctc ttctcatgca gcggttgaca taactcatca
      481 atgtcacatg cctccggaat cattaatttt tctccgtaat acagatgttc caaatgcttg
      541 gaacccatta ccaaaaaggc atagcttgta tgttttgtct caagtatata acatgttttc
      601 ccgtgataaa tcatttccga tccctctctt tatcatgccg ctctttcagt tctcctgtta
      661 tcttctgcag catggtctca tttaatttat atttattggt aaaaaagctg atggataaaa
      721 tcaatccgat cattggaatc accagcatga tcaaacgcag gccaagcagt gtgcctgcgg
      781 tttgtgtgac tgcggtcttg tccagtctga tcagatcgat cccgacaccg gcgataagaa
      841 cggaaaatgc ggatgctaat tttactacaa acgtctgcag agaaaatacc acactctcgt
      901 ttctggcatg attcttccac tccccatagt cgaccgtatc cgccagaaaa atagtcgtaa
      961 gaacagtcgc caaaccaaat ccgcagaaaa taatgatagc ggcaatacat aataatatca
     1021 gattcgttat attcagcgtt cctaaaataa acaacatcat atatccggac agcgccgtta
     1081 tgatcgcacc cttcaggatc tttttgcatt cccatttttt gcgcagggcc ggaaggctca
     1141 tcatggataa gatctgcgcc gcgcctccta ccgtaccgaa aataccatac agactatcct
     1201 gtccaatttt atatttaaaa aaataaatgg caagctgctg cgtcagatac agcgacgcat
     1261 tgaatacaat gatcgtaacc actacgatca gtgcctggtc attatgcagc agggaagaaa
     1321 acatctcctt catccgcaca ctctttttct ttatccggac atcctctttt acattgatca
     1381 cagtcaccac aatggcggca acaaaaaaca gtgcaaccag cagcgcaaat atttcaaacc
     1441 cttttctttc atcaccgctc cccaaaacag gtgcgagcgc cagcgtcagc gcaaccggta
     1501 ttgcgtaccc aaaccctgca cagcttcttg ctattaccga tatattttcc ctgtccttgc
     1561 cggactgggt gattgcaggg atcatggacc aataaggaat atccatcatc gtgtaagtaa
     1621 ttccccacaa taaatataaa acagatatat agaccagcag tcccgttccc tccaggccct
     1681 taggaacagc aaatacaaaa tagagaacga ccgcattgga aagagtgccg attaataacc
     1741 atggacgaaa tttccccagt tttgtagttg tcttttctac gacaaccccc ataaaaggat
     1801 cattcagggc gtcaaagatc ctggctgcca taaacaatat accgataaat gttgcgctga
     1861 tcccaagaat cgtattgtaa taatacagca ggaatcctgt aaccagtgaa tacaccatat
     1921 cttttccgac tgcgccgatc ccataggata cttttacttt tctgatattc ccattcatct
     1981 tctctttctc ttttcattta ttttttaatt ttgcaaaggc cagaatcatt tggtcctgtc
     2041 gtgataactt caaactgcag tgtttttggc tgtatctgtt ctccttctat cgtcaggctg
     2101 ccggttcctt ctgttcctgt cgttctgaca taaacaccgc ccattccgcc ttttaaactg
     2161 ataacagacg gaccgagcag ccgcaaaggg ccttctgtgg ataatctgag cggctcctga
     2221 taataggaca gcagatttcc attctggtca agcatacgca gacggactgc cgcagcatcg
     2281 caggtacctt tatcatataa tgtatgggaa gagacctttg cctccagaac cagctgttct
     2341 gccggttcct ttatcacggt ttttacgacc gctccgttcc tgatcgcctc gaaccggtag
     2401 gaggtgacct tcccgcccca gtttccgaca tacttataat ataagcgaag tccgtcctct
     2461 aactttagtc tgccgcgcat gatcactttc atcattttta ttttatattt gaacggcaga
     2521 ttattctgtc cgaattttgc gactgcgaac agcacttccg taacagctgc tgcagttttc
     2581 gtgtcatacc cttctccttt ttccatcagt tcgccgataa aatcgtcaat tgcaaccggc
     2641 gggtgcggca tatgcggaaa atgctttcta tccggataaa atgcttttac aaactgatca
     2701 tttttataca gccgtacaca gtctgcattt gtaaaggcat acacggtccc tatgtttccc
     2761 gacggatatt ctccgatcga catattagag ctgatttcca gaatattctg ccgtgtaccc
     2821 tgacttgcat aaactgctgc agcaagtttt ggattccgaa acatatccaa aaccccatga
     2881 taacagatca ggtctccgct cccaaagtcc tgatgcgtat tataatcaaa catacaccag
     2941 ccaaatccgc cggcgatg
//
LOCUS       DAOSYE010000212         2955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24838_length_2955_cov_2.747931, whole genome shotgun sequence.
ACCESSION   DAOSYE010000212 DAOSYE010000000
VERSION     DAOSYE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24838_length_2955_cov_2.747931"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLU43_04670"
     CDS             complement(<1..676)
                     /locus_tag="PLU43_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO27734.1"
                     /translation="MEYSILRLTINMQFGDIENIVYPTLLWDKENIILVDCGFIGSLH
                     ILEEELYQHGLSVNQLTGLVLTHHDHDHMGAAAELKKLNSNMKIYSSSMEAPFISAQE
                     KPLRLCQAEEMQKTLTPEQQDFGEAFCNMLRQVEPVGVDNLLHDGDHMDWCGGCRIIA
                     TPGHTPGHISLLMENDSIIITGDAIALEGNHSIIANPQFTLDIEQATKSLDKLLYMKA
                     HAYYCYH"
     gene            complement(859..944)
                     /locus_tag="PLU43_04675"
     tRNA            complement(859..944)
                     /locus_tag="PLU43_04675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(907..909),aa:Ser,seq:cga)
     gene            1129..2157
                     /locus_tag="PLU43_04680"
     CDS             1129..2157
                     /locus_tag="PLU43_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO27735.1"
                     /translation="MKTTIKDIARECGISVSSVSMALSDKPNRISAQTRAKVRETAER
                     LHYQPNMAAVSLVNKKSKMVGIVVNDIRNTHISSLFMAINKELEEKGYSMICHVLNGE
                     DISSNIKLIELIASENVSALIWAKSLELDMAEENEQLKKDMELLGIPILTMDDFGLTC
                     SGVTVRFDYEQGGYLATKHLLDRGHTRIGCLTGDSNYKVTQERMRGYKKALEEYGLPY
                     LKELIYFGNYTMEAGYQALSYLMGQNVTAVFSFNDEMAFGLYQAARNYGVRIPKDVSV
                     IGFDNVPFADVMEVPLSTVQVPIVEMGTFMGKEMISLMEAETSVPRKTVLYKPDLLLR
                     ASTAGRDR"
     gene            complement(2208..>2955)
                     /locus_tag="PLU43_04685"
     CDS             complement(2208..>2955)
                     /locus_tag="PLU43_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO27736.1"
                     /translation="RSMKAVDLVKSLYEPHAVTIILLTVREDIASLFSAKEFEKVKKE
                     MKSTLDAVADQMPGFQVKKEVVIGRAGEEILNCANQNNVDTIVMTKSTKDGWSQVIGS
                     VASHIVKYAACIVIIVPENGAYDRINRKSVRCKHMDDIVTLAGQISLKPSSCYLPVQA
                     GRCSYQITILEGKMRLNHQGFNPDGDTWNLPPKNRQPDHYDLRDGEEKKIQVEILVGY
                     NHLDQIEVVNPSIKEPLKFHYTAHFDNTEN"
BASE COUNT          832 a          591 c          667 g          865 t
ORIGIN      
        1 cgtgatagca gtaataggcg tgggctttca tatacagtaa tttgtccaaa gatttagttg
       61 cctgttcaat atccaacgta aattgaggat tggcaataat agagtggttg ccctccaaag
      121 cgatggcgtc accagttatg ataattgaat cattttccat aagaagcgaa atatgtccag
      181 gtgtatgacc aggcgttgca ataatacgac aaccaccgca ccaatccatg tgatctccgt
      241 catgcagcaa attgtctacg ccaaccggct caacctgacg tagcatatta caaaacgctt
      301 ctccaaaatc ttgttgctct ggtgtaagtg ttttttgcat ttcttctgcc tgacatagtc
      361 tcaacggttt ttcctgtgcg gaaataaacg gcgcttccat agaagaactg tatattttca
      421 tgtttgaatt tagtttcttt agttcggcag ccgcacccat atggtcatgg tcatgatgag
      481 tgagtacaag acccgtcaac tgattcactg acaatccatg ttgatataat tcctcttcaa
      541 gaatgtgaag tgagccgata aaaccacaat caacaaggat gatattctcc ttatcccaaa
      601 ggagcgtggg gtatacaatg ttctcaatat ctccaaattg catgtttatg gttagtcgca
      661 atatactgta ttccataata gctccccttt gcaaaaacaa gtgtgtttca tcttctttat
      721 acaaataatc cgcaattcaa aacggcttgc gcatttctga ttatttgagt tctgaaatat
      781 tctttgaatc catcttatca cattttttca catataaaaa tacatacctt ctccttggca
      841 gaagatatgt atttctgacg gagtcggagg gattcgaacc ctcgtgcccc ggagggcaaa
      901 cgcatttcga gtgcgccccg ttatgaccgc ttcgatacga ctccttgtgt gtctgaaata
      961 tttgaaaaca gatcagatac aaatgaaatt ataatacaac ccgggtaaag aagtcaaata
     1021 gtaatcgcag attttgagag aaagatagaa tagcctttta aaccgattgg aatcatgata
     1081 aacttaggaa aagatatcgc agaatgatga ataaaggagc cggtcaggat gaaaactaca
     1141 ataaaggata ttgcacgtga atgcgggatt tccgtatctt ccgtttccat ggcattgtca
     1201 gataagccca accgcatatc cgcacagaca agagcaaagg tccgtgaaac tgcagagagg
     1261 ctgcattatc agccgaatat ggcagcagtg agtcttgtca ataaaaagag taaaatggtt
     1321 gggatcgtag tgaatgatat cagaaatacg catatctctt ccttatttat ggcaattaac
     1381 aaagagctgg aggaaaaagg gtactccatg atctgccatg tgctgaacgg ggaagatatc
     1441 agctccaata tcaaattgat cgagctgatc gcttctgaaa atgtttcggc actgatctgg
     1501 gcgaaatccc tggagcttga catggcggaa gaaaacgaac agctgaaaaa ggatatggaa
     1561 ttgctcggaa ttccgattct gacaatggat gattttggtc tgacctgcag cggggtcacc
     1621 gtacgctttg attatgaaca gggcggatat ttggcgacaa agcatctgct ggacagagga
     1681 catacacgga taggctgtct gaccggggac agtaactata aagtaacaca ggaaagaatg
     1741 agagggtata aaaaagcgct ggaggaatat gggctgcctt atttaaaaga gctgatctat
     1801 tttggaaact acacaatgga agcgggatat caggcattgt cctatttaat gggccagaat
     1861 gtaacagctg ttttttcctt taacgatgag atggcatttg ggctatatca ggcggccaga
     1921 aattatggcg ttcggatccc aaaggatgtt tctgtgatcg gatttgataa tgttccattt
     1981 gcggatgtta tggaagtgcc cctgagtact gtgcaggttc cgattgtgga aatggggact
     2041 tttatgggaa aagaaatgat ttcattaatg gaagcagaaa cgagtgttcc gcggaaaacc
     2101 gtattgtata aaccggatct gctgctgcgc gccagtactg ccggcaggga tcgatgatat
     2161 aagcttcgtt ttttaaatct gccgcaggat gttcggtctt gcggttttta attttccgta
     2221 ttatcgaagt gggcagtgta atgaaatttt aaaggctctt ttatgcttgg atttacgact
     2281 tctatctgat caagatggtt atagccgacc agtatttcaa cctggatctt tttttcttct
     2341 ccgtccctta gatcgtaatg gtccggctgt ctgttcttgg gcggaagatt ccacgtatca
     2401 ccgtcagggt taaagccctg gtgattcagc cgcattttcc cttctaaaat tgtaatctga
     2461 taagagcatc ttcccgcctg gactggcaga tagcaggagc ttggcttcag actgatctga
     2521 ccggcaaggg tgacaatgtc atccatgtgt ttacagcgca ctgatttacg gtttatccgg
     2581 tcataggctc cgttttcagg tacgatgata acgatgcagg cagcgtattt tacaatatgg
     2641 gaggcaactg aaccaattac ctgcgaccag ccgtcttttg tggattttgt cattacgata
     2701 gtatctacgt tgttttggtt tgcacaattt aagatctctt ctcctgcacg gccgattacg
     2761 acttcttttt tcacctgaaa gcccggcatc tgatctgcaa cagcgtccag cgttgatttc
     2821 atttcttttt ttactttttc aaattctttt gcggaaaaca gtgacgcgat atcttccctg
     2881 actgtgagta aaataattgt caccgcatgt ggttcataca atgattttac aagatcgaca
     2941 gctttcatgc tgcgt
//
LOCUS       DAOSYE010000213         2949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24925_length_2949_cov_3.214927, whole genome shotgun sequence.
ACCESSION   DAOSYE010000213 DAOSYE010000000
VERSION     DAOSYE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24925_length_2949_cov_3.214927"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLU43_04690"
     CDS             <1..1088
                     /locus_tag="PLU43_04690"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO27737.1"
                     /translation="NFFIDNINQLTGSAISEISVTLPLGISFYTFQIVSYIIDLYRGK
                     IKAEHSLLNLGTYLCMFPQLIAGPIVVYGLVAKELQERTYSLEKFEEGIRIFILGLGS
                     KVLIANNVGMLWDDVAQIGYTHISMPLAWLGMLAYTLQIYFDFNGYSLMAIGLGKMLG
                     FDLPQNFNFPYISRSITEFWRRWHMTLSGWFREYLYIPLGGNRRGKARNYLNLFIVWA
                     ATGFWHGAAWNFICWGLLFFVILTAEKEGLGIFLEKHVIFSRVYTLLLIGFSWMIFAI
                     TDPLQIAVYFQRLFSFEPGADAVYYLKNYGVILAIGSILSTPVLKGFYEKKKKSAAGV
                     IASILILLASVAYLTDAAYNPFLYFRF"
     gene            1126..2259
                     /locus_tag="PLU43_04695"
     CDS             1126..2259
                     /locus_tag="PLU43_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOO27738.1"
                     /translation="MMLISLKNIKKYPLFMITFFLLAGVLVLYLLKTDTEFSDMENRY
                     LTVRPALTAAGIEDHTFMEEFETYTEEQIPFRNVLIKAKAAMERMILKEENNGIALGR
                     EGYLFEKVLNVDDQLYENEAYIEKFVGDTDRNISVAIAPNSYEILKQLTPVGFPNIDQ
                     GAELVEFNSRLLEYENCTVIDLQKALAGKEDEQVYYRTDHHWTTDGAYYAYQAYCNMT
                     ENTSVDITSLTAEQIPDFFGTFYAKYKGFGVKPDVITYYDIPIESLTLSSGDEREQLY
                     DLSKKEVYDKYAVFLYGNDGLCEIRSANAGNGKSLVIFKDSYANCLIPFLTYNYDTIT
                     IVDLRYYGDSVEKLLGENNTADILFLYNFSFLNEDRHFYRLTS"
     gene            2327..>2949
                     /locus_tag="PLU43_04700"
     CDS             2327..>2949
                     /locus_tag="PLU43_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27739.1"
                     /translation="MKKQIKNEESEKKKRTAVPAAADKNSDNSEDRTQNVEKRSHKER
                     VISVLLVFLCTLLFAVAAGIGLALWDLNKSPHDMLDAFMNKAQEQTTDTADVPEEESV
                     SQDAAGQPEDAEEEEESAEETVPLVSTDETAGIGTIVRAGDTDEMITGSNVSDNSLSG
                     NTVSSDNADPDAGYPLPFSTVDVSYFDDALFIGDSRMEGFGLYSGTNA"
BASE COUNT          850 a          514 c          751 g          834 t
ORIGIN      
        1 gtaacttctt tattgataat atcaatcagc tgacgggaag cgccatatcc gaaatcagcg
       61 tcacattgcc gcttggcatc agcttctata ctttccagat cgtatcctat atcattgact
      121 tatatcgcgg aaaaataaag gcagagcatt cccttctcaa tctcggtaca tacctgtgca
      181 tgtttccgca gctgattgcc ggacctattg tcgtatatgg tcttgtcgca aaagagctgc
      241 aggagaggac gtacagcctg gaaaaatttg aggaggggat ccgtattttt attttaggcc
      301 ttggttccaa agtgctgatc gcaaataatg tcggaatgct gtgggatgat gtggcacaga
      361 tcggctatac gcatatttcc atgccgcttg catggcttgg catgctggcg tatacgctgc
      421 agatctattt tgattttaac ggctattcgc tgatggcgat cggactcggc aaaatgcttg
      481 ggtttgatct gccgcagaat tttaattttc catatatatc gcgttcgata acggaattct
      541 ggcgcagatg gcatatgact ttaagcggct ggttcaggga gtatctttat attcctctcg
      601 gaggaaacag aagaggaaag gcaagaaatt atctgaatct gtttattgtc tgggcagcga
      661 ccggtttctg gcatggtgcg gcctggaatt ttatatgctg gggcctgctt ttttttgtaa
      721 tattgacagc agaaaaagag ggattaggaa tttttcttga aaaacacgtg attttttcca
      781 gagtctatac gctgcttttg attggattca gctggatgat ctttgcgatc acggatccct
      841 tgcagattgc cgtttatttt caaagactgt tcagttttga accgggtgcg gatgctgttt
      901 attatttgaa aaattacggg gtaatattgg caatcggcag tattttatcg actcccgtat
      961 taaaaggctt ttatgagaaa aaaaagaaaa gcgcagccgg ggtcattgcc agcatactga
     1021 ttctgcttgc atcggttgcg tatctgacag atgccgcata taatccgttt ttatacttta
     1081 gattttagcc ggtgattact gccaatttga taaaaggaca gggatatgat gctgatcagt
     1141 ctgaaaaata taaaaaaata tccgttgttt atgatcacat tttttttact tgcgggtgtg
     1201 cttgtgctct atttattgaa aacagatacg gaattttccg atatggaaaa tcggtatctg
     1261 acagtccggc cggcattgac ggcagccggt attgaagatc acacatttat ggaagaattc
     1321 gaaacatata cggaagagca gattcctttc agaaatgtgc tgatcaaggc aaaagcggca
     1381 atggaacgga tgattttaaa agaggagaac aacgggattg cactaggaag agaggggtac
     1441 ctgtttgaga aagtattgaa cgtggatgac cagctttatg agaatgaagc ctatattgaa
     1501 aagtttgttg gggatacgga cagaaatata tcagttgcga tcgcgccgaa ctcttatgaa
     1561 atattaaagc agctgacgcc cgttgggttt ccgaatatag atcagggcgc agagcttgtc
     1621 gaatttaaca gccgtctttt ggaatatgaa aactgcacgg tgatcgatct gcaaaaagcg
     1681 cttgcgggta aagaggatga acaggtctac tatagaacgg atcatcactg gacaacggac
     1741 ggagcttatt acgcgtatca ggcatactgc aatatgacag aaaatacatc agttgacata
     1801 acgtctctta cagcagagca gatcccggat ttttttggga ccttttatgc aaaatataaa
     1861 ggatttgggg tgaaaccgga tgttataacg tattatgaca ttccgatcga atcactgaca
     1921 ctgtcttcgg gtgatgaaag ggagcagtta tatgatctgt caaaaaaaga agtctatgat
     1981 aaatatgccg tatttttata tggaaatgac gggttgtgtg agatccgatc tgcgaatgcc
     2041 gggaatggga aaagccttgt gatttttaag gactcctatg cgaactgcct gattccgttt
     2101 ctgacttata actacgatac gatcacgatc gtggatttga ggtattacgg tgacagtgtg
     2161 gaaaaattgc ttggggagaa caatacggcg gatattctgt ttctgtataa tttttcattt
     2221 ttgaatgaag acaggcattt ttatcgattg acaagttaaa atacagaggc tattctaatg
     2281 tgcaggggaa gtattgtgat gattaataga tgggacagga taaattatga aaaagcagat
     2341 aaaaaatgaa gaatccgaaa aaaagaagag aacggccgtt ccggctgcag cagataaaaa
     2401 ttcggacaat tctgaagata gaacgcaaaa tgtggaaaaa aggagtcata aagaaagagt
     2461 gatatcggtg ctgcttgtgt ttttatgtac gctgcttttt gcagtggcgg cgggaatcgg
     2521 acttgcttta tgggatctta ataagtcgcc gcatgatatg ctggatgcgt ttatgaataa
     2581 agcgcaggaa cagacaacag atacggctga tgtgccggaa gaggagtctg tttcacagga
     2641 tgctgccgga cagcctgagg atgccgaaga agaggaagaa tctgctgaag agacagttcc
     2701 cttagtatct acggatgaga cggccgggat cgggacgatc gtaagggccg gtgatacgga
     2761 tgaaatgata acaggaagca atgtgtccga caacagtttg tctggaaata ccgtatcttc
     2821 agacaatgct gatccggatg ccggatatcc gcttccgttt tccacagtcg atgtcagcta
     2881 ttttgatgac gcgctgttta tcggagattc caggatggaa gggtttggac tgtattccgg
     2941 aacgaacgc
//
LOCUS       DAOSYE010000214         2947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24952_length_2947_cov_3.476833, whole genome shotgun sequence.
ACCESSION   DAOSYE010000214 DAOSYE010000000
VERSION     DAOSYE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24952_length_2947_cov_3.476833"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLU43_04705"
     CDS             complement(<1..198)
                     /locus_tag="PLU43_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOO27740.1"
                     /translation="MEEIEISLKSIQKTGKQISGNLKLLLLIKGQLEVSVCDKVYQMK
                     PNQILLIDRNQPHSLRGSHQNV"
     gene            complement(481..1116)
                     /locus_tag="PLU43_04710"
     CDS             complement(481..1116)
                     /locus_tag="PLU43_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOO27741.1"
                     /translation="MKIYIADVTLPEIAYMEGLVSKARIDRSKKYKFEIDRRRSLGVE
                     ALLNYAVRSTYPSVPIPVRLMADEHKKPHLIGEHIEFSLSHSGNYAACVIADVPVGID
                     IERHRSDNQGVARRFFTRRECADITDDERFYTYWTLKESFVKAVGQGLSLPMNSFEVM
                     LGKAGEPVAYTQHINEENYFGMTYSIEQGYSLAVCARGIKELPAEIEVAAL"
     gene            complement(1190..2419)
                     /locus_tag="PLU43_04715"
     CDS             complement(1190..2419)
                     /locus_tag="PLU43_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOO27742.1"
                     /translation="MITKKYRICADILNLVMILLLCTILCHMAAVSVSYEFDVTVVYG
                     LAVLLTILYLARLHVKNLFLFIGLHLILTAVLFVLPLPLFVQVELIVFTVILSIADFA
                     FWADRTLHGFFYVHLAWCVIFLAAYLYGSYKQTVNLQDASYVMGILFFGIYFLRLYFE
                     NGMHFSMDKQMNEEVPVRQMYTQNIKMILPLVMVFVAGMFLLQSKVLAQFLMVILRTI
                     FNAVVYFVNWLSSLFPDAGTVTQEAAELPEDFFEKGGASAGWLQALIRIIELIISTAV
                     LLVFLFFSVRGICRFIRKNLHRKEIEAQMLEYQDMKEKRERIVRENMKKKKRLFEKAT
                     NAEKIRRMYRKKIRILTRTGYQVNEADTPMERAEDIFLCKGEDIAEATCIYEEARYGK
                     EAVSDESVRRMKEASYR"
     gene            complement(2409..>2947)
                     /locus_tag="PLU43_04720"
     CDS             complement(2409..>2947)
                     /locus_tag="PLU43_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO27743.1"
                     /translation="STARTGALQVNTFHTTFSKKTVILLNLECNAVAHAQQLQEEAIR
                     IAAYFADRFIREKVPVAFYTNGKDLIMDEPFRIDAGSDRTHIRSIEIALARLDTAKRP
                     VSFTELLDKQVRPEDGAVEYLLISNYRKKDLCDKYAELKKQGYSMRLLVPEFSYNSLE
                     PALCDDQSVMKWTVQNDY"
BASE COUNT          842 a          744 c          490 g          871 t
ORIGIN      
        1 aacattctga tggcttcccc gaagtgaatg cggctgattt ctgtctatta acaggatctg
       61 attcggtttc atctgatata ctttatcaca aacagatact tcaagctgac ccttaattaa
      121 aagtaagagt ttcaaattgc cgctgatctg ttttcctgtt ttctgaatac tttttaaact
      181 gatttcgatc tcttccatct catcccccaa atgaacttaa tcgttttcgt tcttgaattg
      241 aatatctcca gtgtattcaa cttttttatt atagcttaat tttttttagt tatatgtctg
      301 ttttttatca tatttttact attgtttcca cagtatttaa ccgatattat cagttatgca
      361 aaaagcaacc gttacttttt aatatcagcc tgaaaacctg ccttattata caaaaaaacg
      421 aggtgttctt cgtcatttta tgactttcca aacatcctcg ctttattaaa gttcgttaca
      481 ctataatgca gcgacctcaa tctctgccgg cagttctttt ataccgcggg cacagactgc
      541 aagggaatac ccctgctcta tagaatatgt catgccaaaa taattctctt cattgatatg
      601 ctgcgtataa gcgaccggtt caccggcttt tcccagcatc acttcaaaag aattcatcgg
      661 aaggctcagg ccctgcccga ccgccttcac aaaactctcc tttaatgtcc agtatgtgta
      721 aaaacgctca tcgtccgtaa tatcggcaca ttccctccgt gtaaaaaatc gccttgccac
      781 accctgatta tcgctcctgt ggcgttcaat atcgatcccg accggaacat ctgcaattac
      841 acaggcagca taattaccgg aatgtgataa actaaattca atatgttccc ctatcagatg
      901 cggtttttta tgctcgtctg ccataagccg gacaggtatc ggcacagacg gataagtact
      961 tctgaccgca taatttaata atgcttccac gccaagggag cggcgccggt caatttcaaa
     1021 cttgtatttt ttgctgcggt cgatccttgc ctttgaaaca agtccttcca tatacgcaat
     1081 ttcaggcagc gtcacatcag caatataaat tttcatttct tttccttccc aatatatcaa
     1141 cccgttgttt tttattttac accaatcatt tcattaaggc aaatgttctc tatcggtacg
     1201 acgcctcctt cattcggcgg accgattcgt ccgataccgc ctcctttcca taccttgcct
     1261 cttcataaat gcaggtggct tccgcaatat cttcgccttt gcataaaaaa atatcttccg
     1321 ctctttccat cggcgtatct gcctcattga cctgataccc ggtccgcgtc agtattctga
     1381 ttttctttct gtacatcctg cgtattttct cagcattagt cgccttttca aacagccttt
     1441 ttttcttttt catattttcc cggacgatcc gttcccgctt ttctttcata tcctgatatt
     1501 caagcatctg cgcttcgatc tctttcctat gcagattttt tcggataaac cggcagattc
     1561 ccctgacgga aaaaaacagg aaaactaaaa ggacagccgt actgatgatc agctcaataa
     1621 ttctgatcaa agcctgcagc cagccggcgg atgcccctcc tttttcaaaa aaatcctccg
     1681 gaagctccgc tgcttcctgt gtcacggtcc ctgcatccgg aaaaagagaa ctaagccagt
     1741 ttacaaaata tacgacagca ttaaatattg tccgtaaaat caccatgaga aactgcgcaa
     1801 gtactttcga ctgcagcaaa aacatgcctg ctacaaatac cataacaagc ggcaggatca
     1861 tttttatatt ctgtgtatac atctgtctga ccggtacttc ctcgttcatc tgcttatcca
     1921 tggaaaaatg catgccattt tcaaaataca gcctcagaaa ataaatacca aaaaaaagaa
     1981 ttcccattac ataagaagca tcctgcagat ttaccgtctg tttataactg ccatatagat
     2041 aggcagccag aaaaataaca caccatgcca aatgaacata aaaaaagccg tgcaatgtcc
     2101 ggtccgccca aaacgcaaaa tccgcgatcg acaaaatcac agtaaaaacg atcaattcca
     2161 cctggacaaa aagcggcagc ggaagtacaa aaagaacagc tgtcaaaata agatgcagac
     2221 caataaacag gaacagattt ttcacatgca atcttgccag atagagaatc gtaagcagaa
     2281 ctgcaaggcc ataaaccacc gttacatcaa attcatacga aactgaaaca gcagccatat
     2341 gacataatat cgtacaaagc agaagaatca tgaccagatt cagaatatcc gcgcagatcc
     2401 tgtatttttt agtaatcatt ttgcaccgtc catttcatta cgctctgatc gtcacataaa
     2461 gccggttcca aagaattata ggaaaactcc ggtactaaaa gtctcatgga atatccctgc
     2521 tttttcaatt ccgcatattt gtcacacaga tctttttttc tgtagttgga aatcagcaga
     2581 tattcaacag ctccgtcttc gggcctgacc tgtttgtcca aaagttccgt gaacgaaacc
     2641 ggcctctttg cagtatccag tctggccagc gcaatctcaa tacttctgat atgcgtcctg
     2701 tccgaacctg catcgatcct gaacggttca tccatgatca gatcctttcc gttcgtataa
     2761 aaagcgaccg gcaccttttc cctgataaat cgatcggcaa aataagccgc gatcctgatc
     2821 gcttcctcct gaagctgctg cgcatgcgca acagcattgc attccagatt cagcaaaata
     2881 acagtctttt ttgaaaaggt cgtatgaaat gtattgacct gcagcgctcc ggtacgcgcc
     2941 gtacttt
//
LOCUS       DAOSYE010000215         2945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24974_length_2945_cov_3.088235, whole genome shotgun sequence.
ACCESSION   DAOSYE010000215 DAOSYE010000000
VERSION     DAOSYE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24974_length_2945_cov_3.088235"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="PLU43_04725"
     CDS             complement(<1..1823)
                     /locus_tag="PLU43_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOO27744.1"
                     /translation="MKTAQYEIYTEEPARRWDEAFPVGNGQIGAMVYADAPAHAIDLS
                     ENTFYSGRREMDNFQETAAVSFQKMREYAKCGDYRNVQKEAEHFIGIRGNYGTNLPVG
                     KLLINYDTGKENLLFDGRSLNIRTGAAKSRFHVLTKGGTEQRIEETVFASHPDHMLAV
                     WIRSDMPIGVTFSLELSAYGKTEYVKEQDQTGCLQFRTAAYETMHCDTLCGVTLDGDL
                     HVVTDGCVGHDSAALSVSDASVVFAFLTAHTDFCKQMGWPPHDRADTRLNTGDAARIY
                     DRHCEDMKRHMERSSLQITSADGQDLQIPFMYQYGRYLLLCSSREDSFLPAHLQGIWN
                     DNVACRIGWTCDMHLDINTQMNYWPADVTNLPETLYPAMHWLKDAVAAAGAVTAEKCY
                     GLPGWVGELVSNAFGYAFPYWASPIAPCPTGGVWLLTQFYEHYRYTKNRAELVQELFP
                     LLRSAVLFFHAYIFEDENGYYTSGPSISPENSFVKDGTSYQISIGCTYEILMIRELFT
                     MYLEAAEETGNAGEPLYEKVRRQKEKLPAYRIKEDGTIAEFSDGLLVPDLQHRHTSHL
                     LGLFPFSQLTPEKTPELCEAAQKTIRQKTTPIKNWEDTGWA"
     gene            complement(1823..2650)
                     /locus_tag="PLU43_04730"
     CDS             complement(1823..2650)
                     /locus_tag="PLU43_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27745.1"
                     /translation="MGKRKKIVRVLLKQLLCIFLALIVVVPFYMVLINSFKSKSEAAR
                     MSLSLPKEWLFSNYTEVIEKGKLIQGFTNSLLYAGVATFVGVLLCAMAAFVLSRRPGR
                     LNNFIYYFVLCGLFFPVNYVTLIKVLQVVHLTDTRLGIILVFTSAMIPFCVFTIRNFV
                     LSVPVEMDEAAVIDGAGPVALFFGIIVPLLKPVLVTCFILQFMGVWSDFLTPLYLSSK
                     SKMFPMTMAVYQFFGKNKNYWNYIFADIILTCLPVVIVYLVGQKYIVSGMTSGGVKE"
     gene            complement(2650..>2945)
                     /locus_tag="PLU43_04735"
     CDS             complement(2650..>2945)
                     /locus_tag="PLU43_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27746.1"
                     /translation="ANFWHKLIYVTLPMMTGSIMINLVFGLTYGLKVFDIVYVLTNGG
                     PGHETEVLTTYAFQLYSKGQYGMSTALNALLLFITAVVGVAVVKVMSKKEVQQ"
BASE COUNT          759 a          830 c          611 g          745 t
ORIGIN      
        1 cttgcccagc cggtatcctc ccagtttttt atcggtgttg ttttctgccg gatcgtcttt
       61 tgcgccgcct cacatagttc cggtgtcttt tccggcgtca gctgggaaaa cggaaatagc
      121 ccgagcagat ggctcgtatg acggtgctgc agatccggga ccaacaggcc gtctgaaaat
      181 tccgcaatgg ttccatcctc ttttatcctg tatgcaggca gcttctcttt ttgccgtctc
      241 actttctcat ataagggttc tcccgcattt cctgtctctt ccgccgcttc cagatacatg
      301 gtaaacaatt ccctgatcat aaggatctca taggtacatc cgatgctgat ctgatagctc
      361 gtcccgtcct ttacaaaact gttttccggg gaaatggaag gtccgctcgt ataatacccg
      421 ttttcatctt caaaaatata cgcatgaaaa aacaggaccg ccgaccttaa cagcggaaac
      481 agctcctgca caagctccgc ccggtttttc gtataacggt aatgctcata aaactgtgtc
      541 agaagccaga ctcctcccgt aggacacgga gcgatcggcg atgcccaata gggaaacgca
      601 tatccaaagg cattggagac cagctctccc acccatcctg gcagaccgta gcatttctct
      661 gccgttacgg cacctgctgc ggcaacggca tctttcagcc agtgcattgc cggatacaag
      721 gtttccggca gatttgtgac atcggccggc cagtaattca tctgtgtatt gatatccaga
      781 tgcatatcac aggtccaccc gatcctgcat gccacattat cgttccatat tccctgcaga
      841 tgtgccggca ggaacgaatc ctcacgggaa gagcataaaa gcagataccg gccgtactga
      901 tacataaacg ggatctgcag atcctgtccg tctgcggatg tgatctgcag actgcttctt
      961 tccatatggc gcttcatatc ttcacaatgt ctgtcataaa tccgcgccgc atcgcctgtg
     1021 ttcagccgcg tatcggcccg gtcgtgcggg ggccagccca tctgcttaca aaaatccgta
     1081 tgtgcagtca gaaaggcaaa tacaacggac gcatcggaaa cagaaagcgc cgcggaatca
     1141 tggccgacac agccgtccgt aaccacatgc aggtccccgt caagcgtgac cccgcacaat
     1201 gtatcgcaat gcattgtttc atatgcagcc gtccgaaatt gcagacagcc ggtctggtcc
     1261 tgctctttta catattccgt tttcccatat gcagaaagct ccagtgaaaa ggtcacaccg
     1321 atcggcatat ccgaccggat ccatacggca agcatgtgat ccggatgcga agcaaacaca
     1381 gtttcttcga tcctctgttc cgtcccgccc tttgtcaaaa catgaaaccg gctctttgcc
     1441 gcgcctgtcc tgatattcag actgcgtcca tcaaacagga ggttctcttt tccggtatca
     1501 taattaatca ggagttttcc aaccggaaga tttgttccgt aatttcccct gattccgata
     1561 aaatgctccg cttccttctg cacattccga taatccccgc attttgcata ttcccgcatt
     1621 ttttgaaagg aaacagcggc agtttcctga aaattatcca tttcccttct gccgctgtaa
     1681 aatgtgtttt ccgatagatc gatcgcatga gccggtgcat ccgcgtatac catcgcgccg
     1741 atctgtccgt tcccgacagg aaatgcctcg tcccatctgc gtgccggttc ctccgtgtat
     1801 atttcatatt gtgctgtttt cattattcct tcactccgcc ggatgtcatt ccgctgacaa
     1861 tatatttctg tccgaccaga tatacgataa cgaccggaag acaggtcaat atgatatccg
     1921 caaaaatata attccagtaa tttttattct ttccgaaaaa ctgatagact gccattgtca
     1981 tcggaaacat tttgctcttg cttgataaat agagcggtgt taaaaagtcg ctccacacac
     2041 ccataaactg cagaataaag catgtgacaa gcaccggctt gagcagcggc acgatgatcc
     2101 caaaaaacaa ggctaccggc cccgcaccgt cgatcacagc cgcctcatcc atttccacag
     2161 gcacggacag cacaaaattc cggatcgtaa aaacacagaa cggaatcatg gcacttgtaa
     2221 acacaagaat gattccaagc cgcgtatcgg tcagatgaac gacctgcagc actttaatca
     2281 gtgttacgta attcaccggg aaaaacagcc cgcacagcac aaaataataa ataaaattat
     2341 tcagtctgcc cggtcttctg ctcagaacaa aagcagccat cgcgcacagc aatacgccca
     2401 caaacgttgc aacccccgca tagagaagac tgttcgtaaa tccctgtatc agctttccct
     2461 tttcgatcac ttccgtataa ttggaaaaca gccattcctt tggcagtgac agactcatac
     2521 gtgccgcttc gctctttgac ttaaaagaat tgatcagcac catatagaat ggtaccacaa
     2581 cgatcaacgc cagaaagata cacagcagct gctttaacag gacccgcact attttttttc
     2641 tttttcccat tactgctgca cctccttctt gctcatgacc ttcacaaccg ctacgccgac
     2701 tactgccgta atgaataaga gaagcgcatt cagggccgtc gacatcccgt attgtccctt
     2761 ggaataaagc tggaacgcat aggtcgtcaa tacttccgtt tcatgtcccg ggcctccgtt
     2821 cgtcagaaca tagacgatat caaatacctt taagccgtat gtaagaccga agacgagatt
     2881 gatcataatg gaaccggtca tcatcggcag cgttacgtag atcagcttgt gccaaaaatt
     2941 cgcac
//
LOCUS       DAOSYE010000216         2945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24975_length_2945_cov_2.935294, whole genome shotgun sequence.
ACCESSION   DAOSYE010000216 DAOSYE010000000
VERSION     DAOSYE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24975_length_2945_cov_2.935294"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2945)
                     /gene="carB"
                     /locus_tag="PLU43_04740"
     CDS             complement(<1..>2945)
                     /gene="carB"
                     /locus_tag="PLU43_04740"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOO27747.1"
                     /translation="IKKVLVIGSGPIVIGQAAEFDYAGTQACRALKEDGIEIILVNSN
                     PATIMTDKVMADKIYIEPLNLPTIEKIIEKEKPDSILSGFGGQTGLTLSMQLAKNGFL
                     ESHGVRLLGASPETIDKAEDRQIFKDTMESIGQPCIPSKVETTVEGAVEFAKIIGYPV
                     IIRPAFTLGGSGGGIVDNEAELRQVARTGLQMSPITQILVEKCISGWKEIEFEVIRDA
                     AGNVITVCSMENFDPVGVHTGDSIVIAPAVTLSDKEYQMLRTAALKIITALGVEGGCN
                     CQFALEPESFEYAVIEVNPRVSRSSALASKATGYPIAKVAAKIAVGYRLDEIINEVTG
                     ETYAAFEPTLDYVAVKFPKWPFDKFVYAKRELGTQMKATGEVMAISDTFESALMKAVR
                     GVEISLDTLNMKKMEGYSYDELRHIMTAATDERLFAVFEAMKRGASVKEIHEITMIDE
                     WFLYKLQYFVEYEQELANEGAGLSHGSFLSPKLYLKGKRLGYPDAAIERLSGLKIREP
                     MKPCFHMVDTCAAEFEATTPYFYGIYDSEECGIQNEALEFIERSEKERIIVLGSGPIR
                     IGQGIEFDYACVHCVGSLQRLGYEVVTINNNPETVSTDFDTADRLYFEPLYAEDVLHI
                     IEQEKPYGVVVAFGGQTAIKLTKTLDEHGINIIGTSADAIDMAEDRERFDELLEALQI
                     SRPKGSTVMTEEEALEAANRLGYPVLMRPSYVLGGQNMIIAYRDEDIREYMEIILRHK
                     QDNPVLIDKYLCGREMEVDAICDGKDILMPGIMEHIERTGVHSGDSIAVYPSPQVDKE
                     TAEKIMITTQKLCKGLGAIGMINLQYILMDNQLYVIEVNPRASRTVPYLSKVTGVPMC
                     DLATRASLGEKLADMGFGTGLYKKAPYIAVKVPVFSFEKLIDVDTQLGPEMKSTGEVL
                     GVGETINEALYKGLVAAGCKMQKPGGIFISVRNEDKNEIGEIAKKFAGMEFTIYATPG
                     TANIIEEYG"
BASE COUNT          706 a          851 c          562 g          826 t
ORIGIN      
        1 atcccatact cttctataat attcgcagta cctggcgtcg catagatcgt aaattccata
       61 cctgcaaatt ttttcgcgat ctcaccgatt tcatttttat cctcatttct gacgctgata
      121 aaaatacctc ccggcttctg cattttacag cctgctgcca caagtccctt ataaagggct
      181 tcattgatcg tttctccaac tccaagtact tctcctgttg atttcatttc cggtccaagc
      241 tgcgtatcaa cgtctatcag cttctcaaaa gaaaataccg gtactttgac cgcaatatac
      301 ggcgccttct tataaagtcc cgttccaaaa cccatgtctg caagcttttc accaagactg
      361 gccctcgtag ccagatcaca catcggcacg ccggtcacct tgctcagata cggaaccgtc
      421 cttgatgcac gcgggtttac ttctatcaca tacagctgat tatccatcag aatatactgc
      481 agattgatca taccgatcgc tccgagtcct ttgcagagct tctgagtcgt gatcataatt
      541 ttttccgcag tttccttatc gacctgcggc gatggatata ctgcgatact gtcaccggaa
      601 tgaacacctg tccgttcaat atgctccatg atccccggca tcaaaatatc tttaccgtca
      661 cagatcgcat caacttccat ctctctgccg cacagatatt tatcgatcaa tacgggattg
      721 tcctgtttat gccttaaaat gatctccata tactccctga tatcttcatc tctgtaggca
      781 atgatcatat tttgtccgcc aagtacataa gaaggacgca taagcaccgg atatccaaga
      841 cggttggcag cttccagtgc ttcctcttcc gtcatgaccg tgcttccttt cggcctgctg
      901 atctgaagcg cctccagcag ctcgtcaaac cgttccctgt cctctgccat atcaatggca
      961 tccgcacttg taccgataat attgatccca tgctcatcca gcgttttagt cagcttgatc
     1021 gctgtctgtc cgccaaaagc cactacaacg ccgtacggtt tttcctgttc aataatatga
     1081 agcacatctt ctgcgtacag cggctcaaaa taaagcctgt ccgccgtatc aaaatcagtt
     1141 gataccgttt ccggattgtt attgatcgta actacctcat aaccaagtct ctgaagggaa
     1201 cctacgcagt gcacgcatgc ataatcgaac tcgattccct gtccgatccg gatcggaccc
     1261 gaaccaagca caatgatcct ttccttttcg gaacgctcga taaattccag cgcttcattc
     1321 tggataccgc attcctcact gtcataaatt ccatagaaat aaggcgtcgt cgcctcaaat
     1381 tccgccgcac aggtatctac catatgaaaa cacggtttca ttggttccct gattttcagt
     1441 ccgctaaggc gttcaattgc cgcatccgga tagccaagac gttttccctt caaatacaat
     1501 ttgggtgaga ggaacgaacc atgggacaga cctgcgcctt cattggcaag ctcctgttca
     1561 tactctacaa aatactgcag cttatataaa aaccactcgt cgatcatcgt gatctcatgg
     1621 atctctttga ctgacgcgcc ccgtttcatt gcttcaaaca ctgcaaacag acgctcgtct
     1681 gtcgccgctg tcatgatatg gcgcagttca tcataagaat atccctccat ctttttcatg
     1741 ttcagcgtat ccagtgagat ctctaccccg cggactgcct tcatcagcgc agactcaaac
     1801 gtatctgaaa tcgccatgac ctcgcctgtt gccttcatct gcgtaccgag ctccctcttt
     1861 gcataaacaa atttatcaaa cggccatttc gggaacttca cggctacata gtcaagcgtc
     1921 ggctcaaatg cggcatatgt ttcaccggtc acttcgttaa tgatctcatc aagccggtaa
     1981 ccgactgcga tctttgccgc tacctttgcg atcggatagc ctgttgcctt ggacgcaagt
     2041 gccgaagaac gcgataccct tggatttact tcaatgactg catactcaaa tgattccggt
     2101 tccagtgcaa actggcaatt gcagccgccc tcgaccccaa gtgccgtaat gatcttaagc
     2161 gctgccgtac gcagcatctg atactcttta tccgacagtg ttaccgccgg tgcgatcacg
     2221 atactgtccc cggtatgtac accgaccgga tcaaagtttt ccattgagca gactgtgatc
     2281 acattgcctg ccgcatcacg aataacttca aattcgattt ccttccatcc tgaaatacac
     2341 ttttccacaa ggatctgtgt gatcggggac atctgcagac ctgttcttgc gacctgccgc
     2401 agctctgcct cattatccac gatcccgccc ccgcttccgc caagtgtaaa tgcaggacgg
     2461 ataatgaccg gataaccgat gatcttcgca aattccaccg caccctccac tgtcgtttca
     2521 actttggacg ggatacaggg ctgtccgatc gactccatcg tatccttaaa tatctgtctg
     2581 tcttccgcct tgtcgatcgt ttccggagat gccccgagca gacgcactcc atgggattct
     2641 aaaaatccgt ttttagcaag ctgcatggaa agtgtcagac cggtctgccc tccaaatccg
     2701 gacaaaatac tgtccggttt ttctttttct ataatttttt ctattgtagg caggttcagc
     2761 ggctcaatat agatcttatc cgccatgact ttgtccgtca tgatcgttgc cggatttgaa
     2821 ttgacaagaa tgatctcgat accgtcctcc ttgagcgcac gacatgcctg cgttccggca
     2881 tagtcgaatt ccgcagcctg accaatgacg atcggacccg aaccgatcac cagtactttt
     2941 tttat
//
LOCUS       DAOSYE010000217         2944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_24989_length_2944_cov_2.814123, whole genome shotgun sequence.
ACCESSION   DAOSYE010000217 DAOSYE010000000
VERSION     DAOSYE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24989_length_2944_cov_2.814123"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..555)
                     /locus_tag="PLU43_04745"
     CDS             complement(199..555)
                     /locus_tag="PLU43_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27748.1"
                     /translation="MKRVLITDDANFMRLSLKQILTKNGYEIAGEAENGEVAIKLYRE
                     LKPDIVTMDITMPVLGGLDALRGIMALDSNAKVIMISAMGQEHLVRESIMGGARSFIV
                     KPFKEDVVIRALSQFV"
     gene            complement(604..1215)
                     /locus_tag="PLU43_04750"
     CDS             complement(604..1215)
                     /locus_tag="PLU43_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO27749.1"
                     /translation="MNVNKSTDISRRIREAAFILFLEKGYEGTNQRETAEAAGYKASS
                     IYFYYPSKKELFLSVLDEVWEMHMERIQNCLHSKNEFGDDIAKELFVNRINIIRDDYA
                     SYKFLLRFRLFPPVELVGEIREIYMNWEKKQKELYISEIEKWISHTGGGGKVSSEKIY
                     QHFIKIQNYIINDIIVTGEFVSDQELEAHWNRFISLFFDELSE"
     gene            complement(1408..>2944)
                     /locus_tag="PLU43_04755"
     CDS             complement(1408..>2944)
                     /locus_tag="PLU43_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27750.1"
                     /translation="ECGSIGIWDYNIPMDRIEIYNHIGNKKYLIESSKSFIEQIARED
                     VRVLTESFRNISKTNDQFNIDIRLAKNGDASVRWFCMKGKAIEFDKQDKPVRIVGTME
                     DLSVRKDLEKEIMGKNKELKLMIDVANEACQAKSKFLANMSHEIRTPLNGIMLAVNGI
                     KKESLSKNQTKLFSIIENSCTLLNGIVSEILDFSKIERGKVSVADEKVDMSGILQNAV
                     NEIQMAANQKGLETSFYFDPRVQRQFWGDAQKTKQILNNLISNAVKFTYEGGIGLKAK
                     LISEDETHVKILFEVKDSGIGIKTEMQDKVFEPFFQSEETSAKPSPGTGLGLLISKKY
                     AEAMGGDLTFESQEKKGSIFRFQCSFRKEVIAGSILGEPEVMEQPVPKQEDRLEPIDK
                     KRVLCVEDNMVNQELLVATLEEAGYYVDAAFSASEALNCMLENRPDIILMDIQLPLKS
                     GYELAEEIKSMSEYREIPIIAVTAYARKEDRGKCLKAGMVDYVVKPIKIDELFEKIEK
                     YIG"
BASE COUNT          780 a          751 c          423 g          990 t
ORIGIN      
        1 cctttgatcg tatgcatgat tcgaaacact gtctgaatgg catcatccgt atacgaattc
       61 tcttcttcat tcgacaatac gacttcttcc agctgctcta tcagctgtgt tgtttcaaaa
      121 acaaatgctt ccagtaaaac ctcatttgta ttttcatcag tcactctttc accttctctc
      181 cgctatgcat acaccgccct acacgaactg acttaacgcc ctgatcacaa catcttcctt
      241 gaacggtttg acaataaacg accttgctcc gcccataatg gactctctta caagatgctc
      301 ctgccccata gcggatatca taattacctt tgcattcgaa tcaagcgcca tgatccccct
      361 tagggcatcc agacctccga gtacaggcat cgtaatatcc attgtaacga tatcaggctt
      421 taactctctg tagagcttga tcgccacttc cccattttcc gcttcgccag ctatttcata
      481 gccatttttg gtcaatatct gtttcagcga cagcctcata aaatttgcat catctgttat
      541 cagtactctt ttcatgcttt acctcctttg atttgcatgc ctgccgattg ttctctgaaa
      601 acattattcc gataattcat cgaaaaaaag agagataaat ctgttccaat gtgcttccaa
      661 ctcctgatca cttacaaatt cccctgtaac aataatgtcg ttaataatat agttttgtat
      721 tttaataaaa tgctgataga ttttttcgct gctgaccttt ccgcccccgc ccgtatggct
      781 gatccatttt tcgatttcag aaatatagag ctccttttgt tttttttccc agttcatgta
      841 gatctctctg atctccccaa ccagttccac aggcggaaac agccggaacc ttagtaaaaa
      901 cttataagac gcataatcgt cccgtataat attgattctg ttcacaaaca attccttcgc
      961 gatatcatct ccaaattcat ttttcgaatg taaacaattc tgaatacgtt ccatgtgcat
     1021 ttcccagact tcatccaaaa cacttaaaaa cagctctttt ttggaaggat aataaaaata
     1081 tatggaagaa gccttatagc ctgccgcctc tgctgtttcc ctctggttag tcccctcata
     1141 tcctttttcc aaaaacaaaa taaatgctgc ctctctgatt cgtctggaaa tatccgtgct
     1201 tttatttaca ttcatatttc tgcttcctgc ctccataaat ttaacttttg aacgattgtt
     1261 ctatttttcg aacgatcgtt ttataaatat tattatagca aatattattt ttaaattcaa
     1321 ggaatatttg taaaacaata aataaaaaag taaaaaaaaa agaaagaccc caaatgtcac
     1381 aaaatgacaa ttgagatctc ctctgtttta gccgatatat ttctcgattt tttcaaataa
     1441 ttcatctatt ttaattggct tcaccacata gtcgaccatc cccgctttca ggcattttcc
     1501 ccggtcttcc tttctggcat acgccgtcac cgcaatgatc ggaatttccc ggtattcaga
     1561 catgctttta atttcctccg caagctcata cccgcttttc aacggaagct gtatatccat
     1621 caaaataata tccggacggt tttccagcat gcagtttaat gcttccgaag cagaaaaagc
     1681 agcatccaca taatagccgg cctcttcaag cgtcgcaaca agcagttcct gattcaccat
     1741 gttgtcttcc acacacagta ctcttttttt atcgatcggt tccagccggt cctcctgttt
     1801 cggcacaggc tgttccatga cttccggttc ccctaaaata ctgcctgcaa taacttcttt
     1861 cctgaagctg cattgaaatc tgaagatcga gcctttcttc tcctgacttt caaacgtcag
     1921 gtcgcccccc attgcttccg catatttttt tgaaatcagc agtccaagcc ctgttcccgg
     1981 actcggcttt gcacttgtct cttcgctttg aaaaaacggc tcaaatacct tatcctgcat
     2041 ttccgttttg ataccgatcc cggaatcttt tacttcaaat aatattttca catgtgtctc
     2101 atcttcactg ataagctttg cttttagccc tattccacct tcataagtaa attttacggc
     2161 attagaaatc aaattattta aaatctgttt tgtcttctgg gcatctcccc aaaactgccg
     2221 ctgtacccgc ggatcaaaat aaaaacttgt ttccagtcct ttttgatttg ctgccatttg
     2281 aatttcattc accgcattct gcaaaatacc ggacatatcg actttctcat cagcgaccga
     2341 cacttttcct ctttcaattt tagaaaaatc caaaatctcg gaaacaatac cattcaacag
     2401 ggtacagctg ttttcgataa tagaaaacaa tttagtctga ttttttgata aagattcttt
     2461 tttgatccca ttaacagcca gcatgattcc gttgagcggc gttctgattt catgactcat
     2521 atttgcaaga aacttgctct ttgcctggca ggcttcattc gcgacatcga tcatcagctt
     2581 cagctcttta tttttcccca tgatctcttt ttccaaatct tttctgacag aaagatcttc
     2641 cattgttcct acgattctga ccggcttatc ctgcttgtca aattctatgg ctttcccttt
     2701 catacagaac catctgaccg acgcgtcccc attcttcgca agccggatat caatattaaa
     2761 ttgatcattc gtcttactga tgttacgaaa actttccgtc aataccctga cgtcttccct
     2821 tgcaatttgt tcaataaagc tttttgaact ttctatcaga tattttttgt ttccgatatg
     2881 attatagatc tcaatacgat ccatcggaat attatagtcc catatcccga tgctgccgca
     2941 ttcc
//
LOCUS       DAOSYE010000218         2942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25007_length_2942_cov_4.077936, whole genome shotgun sequence.
ACCESSION   DAOSYE010000218 DAOSYE010000000
VERSION     DAOSYE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25007_length_2942_cov_4.077936"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLU43_04760"
     CDS             <1..1287
                     /locus_tag="PLU43_04760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO27751.1"
                     /translation="LDKALYFDYDESSNQSVNAERRILFLQKLNFFSMTEPEKIVRIE
                     LDYSSMTQDLVKMNYDATVYVCWNDKIVLSNGKHSSVGKDFEEFEDWEHVGYEQDASL
                     YGVDLKIYVIQSETKILSAIIKNSPVILLLILVNTILPLILVGMFNRSFTLRIGELSQ
                     AFEKVENETLVEIEDIRGQDEIGSLMRNYNKLAVRINDLIQIVYKNKIREQEMTVARQ
                     NAELLALYSQINPHFLFNALESIRMHSVIKREMETADMVEKLAIMQRQYVEWGNDIID
                     IVKEMEFVRAYLGLQKYRFGDRLSYELDIAKDCSECKIPKLTIVTFVENACVHGIESK
                     TAPGWIFVRIYKNEDWLCIEVEDTGQGMTGESMQELLDKMKRADISMLKEKGGVGIIN
                     ACLRLKMVTQNEVHFELDGEEGVGTMVQIKIPFQYV"
     gene            1308..2909
                     /locus_tag="PLU43_04765"
     CDS             1308..2909
                     /locus_tag="PLU43_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27752.1"
                     /translation="MMLKVLLVDDEPFILQGVKLLIDWEKEGFEITATAANGLEALEY
                     LKDNQVDLIIADIRMPLVSGIELLQRIRIDKISDAYFVILSGYADFSYAQQAIRYECT
                     EYILKPVEKESLLNVLREVAAMKHITETNNQKSKHMERAYLARNMIALIQGKYDKLNL
                     DYVKNHMRLSGGTCYIEIELDDGSINEELTDEEKRVYQRKLFDACLEFLKEDSGHCVF
                     DVSGHEKIYDIGFVYCDYLAEKKNLAKDEYLRSFLFFLRKEVCLPVVMLVGKKVPDIS
                     NLARTYGTTCILRSFQGFRERKDIYYYEEEVQVTNSGIVLCKKSLDKLIRAIEENNHI
                     AIRKNVEFFYQEMKQMGYSKETMNLNINYLLFQLIHLATEQDNGVNQEEILRIISEST
                     FEEGIIRGSKAHISRFACEFGEYLAQLGKNVSRGVLGDVEKEIYDHYAENLTLKGLGE
                     KYFINSAYLGQLFRKKYGCSFKDYLNNYRMDQAAKMLVRTEHKIYRIAEEVGYHDLDY
                     FVNRFIGAKGCTPSKYRKQAENKVG"
BASE COUNT          926 a          480 c          784 g          752 t
ORIGIN      
        1 ctggataagg cgctgtattt cgattatgat gagagcagta atcagtcggt aaatgctgag
       61 cgcaggatcc tttttctgca gaagctgaat tttttcagta tgacagaacc ggaaaaaata
      121 gtgaggatcg aactggatta cagcagtatg acacaggatc ttgtaaagat gaattacgat
      181 gcgaccgttt atgtctgctg gaatgacaag atcgtgcttt ccaatggaaa acattccagt
      241 gtcggaaaag attttgaaga gtttgaggac tgggagcatg ttgggtatga gcaggatgcc
      301 agtctttatg gggtcgacct gaaaatatat gtcattcagt cggaaacgaa aattttaagt
      361 gcgatcataa aaaattcgcc ggtaattttg ctgctgattc tggtcaatac gatactgccg
      421 ctgattctgg taggtatgtt caaccgttcg ttcaccttac ggatcggaga actgagtcag
      481 gcctttgaaa aagttgaaaa tgagacgctg gtcgaaattg aagatatacg cggccaggat
      541 gagatcggaa gcctgatgcg caattataat aagctggcag tcagaatcaa tgacctgata
      601 cagatcgtat ataagaataa gatccgcgag caggaaatga cagttgcgag gcagaatgcg
      661 gagctgctgg cgctgtacag tcagatcaat cctcacttcc tttttaatgc actggaaagc
      721 atccggatgc acagcgtcat caagagggaa atggaaacag cagacatggt ggaaaagctg
      781 gcgatcatgc agagacagta tgtggaatgg gggaacgata ttatcgatat tgttaaggag
      841 atggagtttg tccgtgcata tctcgggctt cagaagtacc ggttcggcga ccggctctcg
      901 tatgagctgg atattgcaaa ggactgcagt gaatgcaaga ttccaaagct tacaatcgtg
      961 acatttgtgg agaatgcctg tgtgcacggg atcgaaagca agacggcgcc cggctggatc
     1021 tttgtgagaa tatataaaaa cgaggactgg ctgtgcattg aggtggagga taccggtcag
     1081 ggaatgactg gggaaagtat gcaagagctg ttggataaaa tgaagcgtgc cgatatttcc
     1141 atgcttaagg aaaaaggcgg cgtcggaatt atcaatgcgt gtctgcgttt gaaaatggtg
     1201 acacagaatg aagtgcattt tgagctggac ggtgaagaag gtgtcggaac gatggtacag
     1261 ataaaaatac cgtttcagta tgtctgagca gaaaaaggag gcgaaagatg atgttgaagg
     1321 tattgcttgt tgatgatgaa ccgtttattc tgcaaggtgt aaaattgctg atcgactggg
     1381 agaaagaggg ctttgaaatc acagcaacgg cagcaaatgg tcttgaggca ctggaatatc
     1441 tgaaggataa ccaggtggat ctgattattg cggatatcag aatgcctcta gtttctggta
     1501 tagagctgct gcagaggatc cgcattgata agatatcaga tgcttatttt gtcatattga
     1561 gcggatatgc cgatttttcc tatgcgcagc aggcgatccg gtatgagtgc accgagtata
     1621 tattgaaacc ggtagaaaag gaaagcctgc tgaatgtatt gcgtgaagtg gctgcaatga
     1681 aacatattac agagacaaat aaccaaaaga gcaaacatat ggagcgggct tatctggcca
     1741 ggaacatgat tgccctgatc cagggaaaat acgacaagct gaatcttgac tatgtgaaaa
     1801 atcatatgcg tttatcaggc gggacctgct atattgaaat cgagctggat gacggcagta
     1861 tcaatgagga actgactgat gaagaaaaaa gagtctatca gaggaagctc tttgatgcgt
     1921 gcctggaatt tttaaaagaa gacagcggac attgcgtttt tgatgtatcc ggacatgaga
     1981 agatctatga tatcggtttt gtttattgtg attatctggc agagaaaaag aatcttgcaa
     2041 aggatgagta tttgcgaagc tttctcttct ttttaagaaa agaggtgtgt ctgccggtcg
     2101 ttatgcttgt cgggaaaaag gtgcctgata tcagcaatct ggcaaggacc tatgggacga
     2161 cctgtatttt gcgttctttt cagggatttc gggaaagaaa agatatctat tattatgaag
     2221 aggaagtaca ggttacaaac agcgggatcg ttctctgtaa aaaaagcctg gataaactga
     2281 tacgtgcgat cgaagaaaat aaccatattg caatcagaaa aaatgttgaa tttttttatc
     2341 aggaaatgaa acagatggga tattccaaag agactatgaa tttaaacatc aactatctgc
     2401 tgtttcagct gatccatctt gctaccgaac aggataatgg cgtgaatcag gaggagatcc
     2461 tgcgtattat cagtgaaagt acatttgaag aaggaattat ccgcggaagc aaggcacata
     2521 tatccagatt tgcatgtgaa ttcggagaat atcttgcaca gcttgggaaa aacgtatcaa
     2581 gaggggtatt gggagacgtc gaaaaagaga tctatgacca ttatgcggaa aatctgacac
     2641 taaaaggtct tggggaaaaa tattttatta acagtgccta tcttggacaa ttgtttcgaa
     2701 aaaaatatgg gtgttctttt aaagattacc tgaacaatta tcggatggac caggcagcca
     2761 aaatgcttgt ccgtacggag cacaagatct accggatcgc agaagaagta ggctatcatg
     2821 atctggatta ctttgtaaat cgttttatcg gggcaaaagg ctgtacgccg tccaagtaca
     2881 ggaaacaggc ggaaaacaaa gtcggatgaa aggacagaca ttaaaaaagg tacagggtga
     2941 at
//
LOCUS       DAOSYE010000219         2938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25060_length_2938_cov_4.130073, whole genome shotgun sequence.
ACCESSION   DAOSYE010000219 DAOSYE010000000
VERSION     DAOSYE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25060_length_2938_cov_4.130073"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..780)
                     /locus_tag="PLU43_04770"
     CDS             complement(67..780)
                     /locus_tag="PLU43_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO27753.1"
                     /translation="MEYKIPKYVQIKEDIIKAIKSGELKPGDKVDSESVLKKKYNVST
                     ITVRKAFNSLISEEYLIGVQGLGTFVTRKQMNRKLTSISFSDELLQQGYKIDMHVDKI
                     EEIFNEAIADKLLISHNQPIVCVSRIRLANNEPIAYQSSFMDSSVLSLEQAQKIYEYK
                     SFYKTLSANQIVPVWANENYSVREVKDARIAGLMGIKKNTDTFFVKRNTVGESGKVIE
                     YAETYFNKDWYSVTVEVKI"
     gene            complement(885..1280)
                     /locus_tag="PLU43_04775"
     CDS             complement(885..1280)
                     /locus_tag="PLU43_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27754.1"
                     /translation="MKRVIVMGHGGYAEGIRKNIEMVAGLSSHMYFVDLEKEEDLADL
                     KAKVRELLEGFAEDTDVLFACDLLGASPFRAAAEICIDHPGKYYAAAGLNVMALLEMD
                     LDEGSECTMEAYVNRAIETTKTTVAKFPE"
     gene            complement(1415..2278)
                     /locus_tag="PLU43_04780"
     CDS             complement(1415..2278)
                     /locus_tag="PLU43_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOO27755.1"
                     /translation="MKTISKKTLRKSFFNWFFWNGCSQQAESMLGMAFGQSMAPVIEE
                     LYDKKEDKAAALERHITLFNTESQVGSICNGVVCGMEEANANGKCTPELINSVKVALI
                     GPTSAIGDSLWVATIIPILLTICLSISQASTAISWLGPVIYMLVYPLGTCILSWSLFK
                     LGYKSGIEGMQGVMSTGKLNTFTSTMTVLGLLVVGALTASFVTFTLPIQIVRDVYDAT
                     AEELLENQVIFNADTVVNSIFPSILPLALTLFVYYLYSKKKWSPLKLMGLILVLACVV
                     TLIGYATGFYV"
     gene            complement(2281..2511)
                     /locus_tag="PLU43_04785"
     CDS             complement(2281..2511)
                     /locus_tag="PLU43_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOO27756.1"
                     /translation="MTTVLSVFGGMMAALGMGIILSFLLKKKYQIVIFLAGFILITYF
                     NLSTMAVAVIAIIITVLYYVAITSLEAKKGEA"
BASE COUNT          849 a          704 c          461 g          924 t
ORIGIN      
        1 ctcatatcat tatagttatc tgctttatat agcttcatcc tctgtttccc ctgacattta
       61 aaaattttaa atttttactt ccactgttac ggaataccag tctttattaa aatacgtttc
      121 tgcatactca attacttttc ctgattcccc taccgtattt ctttttacaa aaaaggtatc
      181 tgtattcttt tttatcccca tcaatcccgc aattctggca tcctttactt ccctgaccga
      241 ataattttcg tttgcccaga ccggtacgat ctgatttgcg gaaagtgtct tataaaatga
      301 tttatattca taaattttct gcgcctgttc aagactgagc acgctgctgt ccataaaaga
      361 actctgatat gcaatcggct cattattggc aagccgtatt ctgctgacac atacgatcgg
      421 ctgattatgg gaaatcagca gtttatctgc aatcgcttca ttgaaaattt cctcaatctt
      481 atccacatgc atatcgattt tataaccctg ctgcagcagt tcatcgctaa aactgatcga
      541 cgtaagtttt cgattcatct gttttctcgt aacaaacgtt ccaagaccct gtacgccgat
      601 cagatactct tcactgatca aactgttaaa cgcttttctg actgtaattg ttgaaacatt
      661 atattttttc ttcagtacgg attcactgtc aaccttatcc cctggtttca gctcacctga
      721 tttgattgcc ttgataatat cttcttttat ctgcacatac tttggaattt tatactccat
      781 ttgttataat acctcctaat atattaacat aataactgat cttatattat aacatccctg
      841 tacaatgcgc aatatcctgt attacaggat agaaagatat ctgtttattc cggaaatttt
      901 gccacggtcg ttttcgtcgt ttcaatcgcg cggttcacat acgcttccat cgtgcattcg
      961 cttccttcat ccagatccat ctccaaaagc gccattacat tcaaccctgc tgccgcatag
     1021 tattttcccg gatggtctat acaaatttca gcagcagccc gaaagggcga tgcacctaat
     1081 aaatcacacg caaacagaac gtccgtatcc tccgcgaagc cctccagcag ctcccttact
     1141 tttgctttca gatctgccag atcttcttct ttttctaagt caacaaaata catatgcgaa
     1201 gacagacctg caaccatctc tatatttttt ctaatgcctt ccgcatatcc accgtgtccc
     1261 atgactatga ctcgcttcat tttatttcct tctgcctttc tgtttcagta caaaaactcc
     1321 tgcataaaaa taatttatga cagagaccct gacaacggtt ctgtttcagg tctctgtccc
     1381 gcatgatctg cggtattaca atcctgtgtt tctcctatac ataaaatcct gttgcataac
     1441 cgatcagggt aactacacat gccagtacaa gaatgagccc cattaattta agcggagacc
     1501 acttcttttt agaatacagg taatatacaa acaacgtcaa ggccagcggc aaaatactcg
     1561 gaaaaataga attgacaaca gtatccgcat taaaaatgac ctgattttcc aataattcct
     1621 ctgcagtcgc atcataaaca tccctgacga tctgaatggg aagtgtaaat gttacaaaag
     1681 aggctgtcag cgcgccaaca acgagcagcc caagcaccgt catcgtactt gtaaatgtat
     1741 tgagcttacc tgtagacatc accccctgca tcccttcgat gccggactta tatccaagtt
     1801 tgaataaaga ccagcttaaa atgcatgttc caagcggata aacgagcata taaattacgg
     1861 gaccgagcca ggaaattgca gtcgatgcct gtgaaatgga taaacagatc gtaagcagga
     1921 tcggaatgat tgttgcgacc cataaggaat ctccaattgc agaagtcggt cctatcaaag
     1981 ccactttgac actattgatc aactcaggcg tacattttcc attcgcattt gcttcttcca
     2041 tgccacagac gactccgttg cagatagatc ctacctggga ttctgtgttg aacaatgtaa
     2101 tatgcctttc cagtgcagct gctttatctt ctttcttgtc atacaattcc tcaataacag
     2161 gtgccataga ttgtccgaaa gccattccga gcatactttc cgcctgttga gaacatccat
     2221 tccaaaagaa ccagttgaaa aatgattttc taagtgtttt tttactgatt gtcttcatga
     2281 ttatgcctct cctttcttag cctcaagact tgttatcgca acataataca gaactgtaat
     2341 gataattgct ataacggcaa cggccatcgt acttaaatta aaatacgtga tcaggataaa
     2401 tcctgccaga aaaataacta tttgatattt tttctttaaa agaaatgata gaatgattcc
     2461 cattccaagc gcagccatca taccgccaaa tacactgagt accgtagtca cccaccctga
     2521 taagcgttgc aaaatcggcc tgtgcagatg ccgctgtgat ggacgtcaat acaatcactg
     2581 caggaataaa acgacacgca aacccaatca gctgaccaag cacaagattt ggtatgaaca
     2641 tctttttcgt ttcacctgcc tccgcatatt tatgcgccac aacgttcaaa ggcagattca
     2701 gtgcataaga aacattccac atgatctgtc cacctctcta tataatgtaa ctccgtacat
     2761 actgccgacg gatcataaca cgttcttgat atgttaatat gttaatatgt taacatgtta
     2821 tgttgaattt attatattac agaccttgct tcattacaat agtttttcat catttttttt
     2881 aatttactca aacttcgcac ttgaataagt gtctatgcac cttgaaaatt caataata
//
LOCUS       DAOSYE010000220         2933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25134_length_2933_cov_3.160181, whole genome shotgun sequence.
ACCESSION   DAOSYE010000220 DAOSYE010000000
VERSION     DAOSYE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25134_length_2933_cov_3.160181"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..786
                     /locus_tag="PLU43_04790"
     CDS             247..786
                     /locus_tag="PLU43_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO27757.1"
                     /translation="MQTLDCIRKRRSIRKFTDEPVSHETLEKIVAAASFSPSWKNTQI
                     VRYIAIETPALKNKIASDCTVGFPYNAGTINAAPMLIAVTVMKNRCGYERDGSFSTNR
                     KDTWQMFDAGIASQTFCLAAHELGLGTVIIGIFDDTLSDLLKLPQDRELVNLICIGHP
                     AEDPDVPKRKSVTDLLSYQ"
     gene            855..1769
                     /gene="pyrB"
                     /locus_tag="PLU43_04795"
     CDS             855..1769
                     /gene="pyrB"
                     /locus_tag="PLU43_04795"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOO27758.1"
                     /translation="MRHLMSPLDFSVDELDRLLDLAGDIRQNPEKYAHICDGKILATC
                     FYEPSTRTRLSFESAMMHLGGKVLGFASASSSSAAKGESVSDTIRMISCYADICAMRH
                     PKEGAPMVAASKSTIPVINAGDGGHQHPTQTLTDLLTIRSLKGRLDHLTIGLCGDLKF
                     GRTVHSLINALIRYPDISFTFISPEELRIPNYIREDVLEKNHIPFKEVIRLEDVIADL
                     DLLYMTRVQRERFFNEEDYVRLKDFYILNKAKMELARKDMLVLHPLPRVNEISTEVDN
                     DPRAVYFKQVQFGVYVRMALILTLLELA"
     gene            1796..2230
                     /locus_tag="PLU43_04800"
     CDS             1796..2230
                     /locus_tag="PLU43_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOO27759.1"
                     /translation="MLNVGKIEEGFVLDHIHAGKSLSIYHDLKLNQLDCTVAIIKNAR
                     SNKMGKKDILKVECDINMLDLDILGFIDHNITVNVIKDGVIVEKKTLVLPKEIRNVIK
                     CNNPRCITSIEQELTHVFILTDPEKEIYRCKYCEEQYSEHDL"
     gene            complement(2323..>2933)
                     /locus_tag="PLU43_04805"
     CDS             complement(2323..>2933)
                     /locus_tag="PLU43_04805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOO27760.1"
                     /translation="EGRFREEIVPVEVKKKKETVLVDTDEGPRKGVTVESISRLRPAF
                     KPDGLVTAANSSGINDGAAAIIVMSEEKAKELGITPMATWIAGELAGVDPSIMGIGPV
                     AATRKVLKKTGMQIKDFDLVEANEAFAAQSVAVGHDLGFDMDKLNVNGGAIALGHPVG
                     CSGCRILVTLLYEMKKRQLETGLATLCVGGGMGCAAVVKMEQ"
BASE COUNT          830 a          721 c          611 g          771 t
ORIGIN      
        1 gtatacttcg taaatgtaaa cggaagcgct agaaaaaccc atctctttct ctcgacaaat
       61 ttcatcatat aaaatccttt cccaatgtat ttttactgcc tcttctgcga acaaaagcag
      121 acgttcctgt tttgtcccaa ctatgcacta agtatattac attctgaaaa ataatgcaaa
      181 acatacttga ataagatttt ggaatatatt atactggatg aaatgaaggc aaaaggaggg
      241 catgtcatgc agacactgga ttgtatccgt aaacgcagaa gtatccgtaa attcacagat
      301 gaacctgtat cgcacgaaac tttggaaaag atcgtcgccg ccgcttcttt ttccccttcc
      361 tggaaaaata ctcagatcgt acgctatatt gccatagaga ctcctgcatt gaaaaataag
      421 atcgccagtg actgcactgt cggctttcca tataatgcag gcacaatcaa tgctgctccg
      481 atgctgatcg ctgtcaccgt catgaaaaac agatgcgggt acgaacgcga cggctctttt
      541 tcaacaaaca gaaaggatac gtggcagatg tttgatgccg gtatcgcttc tcagacattt
      601 tgtcttgccg ctcatgagct tggtctcgga acggtgatca ttggtatttt cgacgatacg
      661 ctatccgatc tgttaaagct cccgcaggac agagaacttg taaatctgat ctgtatcggc
      721 cacccggccg aggatcctga cgttccaaag agaaaatccg ttacggattt attatcctat
      781 caataatcaa aacgaagatg ccttttatct tcgttttttt atgtaaacat tataaaaaga
      841 aagaggtaac cactatgaga catttaatga gtccgcttga tttctcagta gacgagctgg
      901 acagactcct tgatctggcc ggtgatatcc ggcaaaaccc agaaaaatat gcacatatct
      961 gcgatggaaa aattcttgca acctgctttt acgagccaag tacaagaaca agactcagtt
     1021 ttgagtctgc catgatgcat ttaggcggaa aggtgcttgg attcgcatct gccagctctt
     1081 cttcggctgc aaagggagaa agcgtttccg atacgatccg tatgatctcc tgctatgcgg
     1141 acatctgcgc catgcgtcat ccgaaagaag gcgcgccgat ggtcgctgct tccaaatcaa
     1201 caattcccgt catcaatgca ggcgacggcg gacatcagca tccgacgcag acactgaccg
     1261 atctgctgac gatccgttct ctgaaaggaa gacttgacca cttaacgatc ggcctgtgcg
     1321 gcgatctgaa attcggccgt accgtacatt ccctgatcaa cgccctgatc cgctatccgg
     1381 acatctcctt tacgttcatt tctccagaag aactgaggat cccaaactat atcagggagg
     1441 atgttcttga aaaaaatcat attcccttca aggaagtgat ccgcctggag gacgtcatcg
     1501 ccgatcttga cctgctttat atgacacgtg tacagcggga acgatttttt aatgaagagg
     1561 attatgtcag actcaaggat ttctatattt taaataaagc aaagatggaa ctggcgcgca
     1621 aagacatgct cgtactgcat ccccttccaa gagtcaatga gatctcaaca gaggtcgaca
     1681 atgatccgcg cgctgtctat ttcaaacagg tacaatttgg agtttatgtc agaatggcac
     1741 tgattctgac actgttagag cttgcatagg caagcgttga taaggaggaa tgacaatgtt
     1801 aaacgttgga aaaattgaag aaggctttgt acttgaccat atccatgccg gaaagagtct
     1861 gtcgatctat catgacttaa agctgaacca gctggactgc accgtagcca ttattaaaaa
     1921 tgccagaagc aacaaaatgg gaaaaaagga cattctgaag gtggaatgtg atattaatat
     1981 gctcgatctg gacattcttg gatttattga tcataatatt accgttaacg tcattaagga
     2041 cggcgttatt gtggaaaaaa agacccttgt actgcctaaa gagatccgca atgtgatcaa
     2101 atgcaacaat ccccgctgta tcacctccat tgagcaggag cttacgcatg tctttatact
     2161 taccgatccg gaaaaggaga tctatcgctg caagtactgt gaagagcagt acagtgagca
     2221 tgatctttaa tatgaacaca ggcagaaata tcaaaaaaga acgataccgc ggaatattca
     2281 tccatgatat cgttctttta ttttctgtca gaaagcagcc tgttactgtt ccatcttcac
     2341 aacggccgca cagcccattc ctccgccgac acataacgtg gcaagtccgg tctcaagctg
     2401 tctttttttc atttcataaa gcagtgtcac aagaattctg cagccggaac agcctaccgg
     2461 atgcccaagc gcgatcgcac ctccgtttac attcagcttg tccatatcaa aaccaagatc
     2521 atgccctacc gcaacagact gtgccgcaaa tgcctcattt gcctctacca gatcaaaatc
     2581 cttgatctgc attcctgtct tcttcagcac tttacgtgtt gctgccacag gtccgatccc
     2641 cataatggac ggatccactc cggccagctc gccggcgatc catgtagcca tcggcgtaat
     2701 gccaagttcc tttgcttttt cctcactcat gacaataatc gctgccgcgc cgtcattaat
     2761 gcccgaagag ttggctgccg ttactaaacc gtccggcttg aaagcaggcc gcagcctgga
     2821 aatactctcc acagtcacgc ccttacgcgg accctcatcc gtatcgacaa gcactgtttc
     2881 cttcttcttt ttgacttcga ccggaacgat ctcctcccgg aaccttcctt ctg
//
LOCUS       DAOSYE010000221         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25222_length_2926_cov_3.273772, whole genome shotgun sequence.
ACCESSION   DAOSYE010000221 DAOSYE010000000
VERSION     DAOSYE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25222_length_2926_cov_3.273772"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLU43_04810"
     CDS             complement(<1..193)
                     /locus_tag="PLU43_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27761.1"
                     /translation="MSMLKITDLQVFYGVIQAIKGIRFEVNEGEVIALIGANGVGKTT
                     ILHTITGLVDAKAGKIEFEG"
     gene            complement(205..966)
                     /locus_tag="PLU43_04815"
     CDS             complement(205..966)
                     /locus_tag="PLU43_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27762.1"
                     /translation="MALLEVKNIGISFGGLRAVDDLNMTVEEGSLVGLIGPNGAGKTT
                     VFNMLTGVYLPTNGIIKLNGKSIVGQKPNEICNEGIARTFQNIRLFSKMSVLDNVKVA
                     LHNEIEYSLLESFLHLGSYIKKEKEMTEKAMAILQVFDLDKQANYKASNLPYGKQRKL
                     EIARALATGPKLLLLDEPAAGMNPNETAELMHTIEIVRNQFHVTILLIEHDMKLVAGI
                     CEYLYVLNFGTLLANGKPEQVLNDPKVITAYLGED"
     gene            complement(969..1925)
                     /locus_tag="PLU43_04820"
     CDS             complement(969..1925)
                     /locus_tag="PLU43_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008754128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO27763.1"
                     /translation="MMKNKKKIIPFIIAVVVYALVMFLVQSGMLSRQMTSIIIPVCVN
                     VMLSVSLCLIIGFLGELTLGHAGFMSIGAYTGALITINTAGSMPVWLSFTIAIIVGGL
                     VAGVLGLLIGMPVLRLKGDYLAIVTLAFGEIIKSIINSLKITNGAAGLSDIPKYSTYK
                     SFTVVYIIAVLMILLVANFVNSRHGRAICSIRDNYIAAEATGLPVSRFKVLAFVISAV
                     FAGIAGVIYAHNVGILKPATFDYNKSIEILVMVVLGGMGNIKGSIIAAIILTLLPEVL
                     RGVDDYRMLMYSIVLIAMMIINNSAIKQTIISKFRKAAKEDN"
     gene            complement(1925..2812)
                     /locus_tag="PLU43_04825"
     CDS             complement(1925..2812)
                     /locus_tag="PLU43_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO27764.1"
                     /translation="MNILNSIIEQLINGLRTGSIYALIALGYTMVYGIAKMINFAHGD
                     IIMVGAYALFVFVTTLHIPVVPSVILTILFCAAVGVVTEKVAYKPLRKAQPLAVLITA
                     IGVSYFLQNFVQLIFGATPIPFASIIQIPAVKINTINISGITIITLVVTTICMILLSL
                     FISKTKAGRAMRAVSEDKGAAELMGINVNGTISLTFAIGSGLGAVAGILFICQYEALR
                     PTLGALPGIKAFVAAVLGGIGSIPGAMLGGIILGIIESLSKAYISTQLSDAIVFGVLV
                     IVLLVRPSGLLGKQRREKV"
BASE COUNT          805 a          679 c          662 g          780 t
ORIGIN      
        1 ttccttcgaa ttctatttta ccggcttttg catctaccag accggtgatc gtatgaagga
       61 tcgttgtttt gccgacaccg tttgcgccga tcagtgcaat gacctcgcct tcgtttactt
      121 caaaccggat tcctttgatt gcctggatga caccgtagaa aacctgcaga tcggtaattt
      181 ttaacatact cataatcgtt cctcctagtc ttcacctaaa taagcagtaa taaccttcgg
      241 atcattgagt acctgttcgg gcttgccgtt tgcaagcagc gttccaaagt ttaatacata
      301 aagatattca caaatgccgg caaccagctt catatcatgc tcgatcagaa ggatcgtgac
      361 atggaactga tttctgacga tctcgatcgt gtgcatcagt tcagctgttt cgttcggatt
      421 catgcctgcg gcaggttcgt caagcaaaag cagcttcggg cccgtggcaa gcgcacgtgc
      481 gatttccagc tttcgctgtt ttccataagg aagattggaa gctttataat ttgcctgctt
      541 gtccagatca aacacctgca ggattgccat tgctttttct gtcatctctt tttctttttt
      601 tatataggat ccaaggtgaa gaaagctttc cagcagggaa tattcgattt cgttgtgcaa
      661 agcgactttg acattatcca gtacactcat tttggaaaaa agacggatat tctgaaaagt
      721 ccttgcgatt ccttcattgc agatctcgtt tggcttttgg ccgacaatgc tttttccgtt
      781 taatttgatt atgccgtttg taggaaggta aacaccggtc agcatattga aaaccgttgt
      841 tttgccggca ccgttcggtc cgatcagacc aacgagcgag ccttcttcaa ccgtcatgtt
      901 cagatcatct acggcacgca ggccgccaaa ggagattcct atgtttttta cttccagtaa
      961 tgccatgctc agttatcctc ctttgctgct tttctgaatt tggaaatgat cgtctgtttg
     1021 attgcggaat tattgatgat catcattgcg atcaatacga tcgaatacat cagcatacgg
     1081 taatcatcaa cacccctgag tacttcaggc agcagcgtca ggataatggc tgctataatg
     1141 gatcccttga tgttgcccat accgccgagt acgaccataa cgagaatttc aatagattta
     1201 ttataatcaa aagtggcagg ctttaagatg ccgacgttgt gcgcataaat aacacctgca
     1261 attcctgcaa agaccgcgga aatcacaaaa gcaagtacct tgaatcggct gaccggcagt
     1321 ccggttgctt ctgctgcgat ataattgtca cggatcgagc agatcgcacg gccatgtctt
     1381 gaatttacga aattggcaac aagaaggatc atcagcaccg caatgatata gacgactgta
     1441 aagcttttat aggtagagta tttcgggata tcggataatc cggcagctcc gttcgtgatt
     1501 tttaatgagt tgataatgga tttgatgatc tcaccaaatg ccagcgtaac aatggcaaga
     1561 taatcacctt tcaggcgaag aacaggcata ccgatcagca gaccgagaac accggctaca
     1621 agtccgccga caatgatcgc gatcgtaaag gagagccata caggcatgct gcctgcggta
     1681 ttgatcgtga tcagggcacc ggtataagcg ccgatggaca taaaaccggc atgtccaagt
     1741 gtcagttctc caagaaagcc gatgatcagg cacagggaaa cggacagcat gacatttaca
     1801 cagaccggaa tgataataga tgtcatctgc cggctaagca tcccgctttg aacaagaaac
     1861 atcacgagcg cataaacaac aaccgcaatg ataaatggaa tgatcttttt tttatttttc
     1921 atcattatac cttctccctt ctctgcttac cgagaagacc ggacggtctt acaaggagaa
     1981 caataactaa cacgccaaaa acgatggcat cggaaagctg cgtggaaata tatgctttgg
     2041 aaagactttc gatgatgccg agaataatgc cgccgagcat tgcgccgggg atcgaaccga
     2101 tcccgccaag cactgccgca acaaaagcct tgatgccggg aagtgcgccg agcgtgggcc
     2161 gcagggcttc gtattgacag atgaacagga tgccggccac cgcaccgagt ccggagccga
     2221 tcgcaaatgt aagggagatc gttccattga cattgatgcc catcagctct gcagctcctt
     2281 tatcctcgga aacagcacgc atcgcgcgtc ctgcttttgt tttactgata aacaatgaca
     2341 gcagaatcat acagatcgtt gtgacgacta aagtgatgat tgtaataccg gaaatattga
     2401 tcgtattgat ttttactgcc ggtatctgga taatggaggc aaacggaatt ggggtcgcac
     2461 caaaaatgag ctgaacgaaa ttctgtaaaa aataactgac accgattgca gtgatcaata
     2521 ctgccagggg ctgcgccttt ctgagcggtt tataagcgac tttttcggta acgacgccga
     2581 ctgccgcaca aaataggatc gtcagaatga ccgacggaac aaccggtata tgcagcgttg
     2641 tgacaaatac gaaaagtgca taggcaccga ccataatgat gtcgccgtga gcgaagttga
     2701 tcattttggc gatcccgtat accatcgtat agccaagcgc tatcagtgcg tagatgcttc
     2761 cggttctcag accattgatc agctgttcga taatgctgtt caggatattc attgttttca
     2821 ccataccttt tgattgatta gtaaggacac atataaatgc agaagacgag agcagtcatg
     2881 ctcccgtctt gcgtcattgc atacctacgt tattataaca taggtt
//
LOCUS       DAOSYE010000222         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25250_length_2924_cov_3.229000, whole genome shotgun sequence.
ACCESSION   DAOSYE010000222 DAOSYE010000000
VERSION     DAOSYE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25250_length_2924_cov_3.229000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLU43_04830"
     CDS             <1..271
                     /locus_tag="PLU43_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27765.1"
                     /translation="DYYYLPEEDCCIHKSAAMAVDKDHRLHAKKETCYTKHTGLFLPQ
                     FSSIITPYFQTEYKTKILYFEYNRDNMTPAAMNQYGTHLLNFFCG"
     gene            complement(285..1067)
                     /locus_tag="PLU43_04835"
     CDS             complement(285..1067)
                     /locus_tag="PLU43_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOO27766.1"
                     /translation="MAYDYEGFKAAILRMTGIDLSSYKEKQMRRRIDTLINKHRISGY
                     EDFISRIRSDKQLFEEFVNYLTINVSEFYRNTDQWELLDKTFIPEMLRQFGKNLKIWS
                     AACSTGDEPYSLVMALSRHLPLNQIKILATDLDKQVIAQAKIGLYAEKSIANVPADLR
                     SKYFKQIGASYQISNEIKSRVEFREHNLLKDHYPDRCHFIVCRNVLIYFTEEAKDEVF
                     QKFSDALVPDGILFIGSTEQIMNHRDFGYERRNSFYYQKKHN"
     gene            complement(1073..2233)
                     /gene="rpsA"
                     /locus_tag="PLU43_04840"
     CDS             complement(1073..2233)
                     /gene="rpsA"
                     /locus_tag="PLU43_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOO27767.1"
                     /translation="MSELTFEQMLEQSFKTIHNGEVVEGTVIDVKPDEIILNIGYKSD
                     GIVTKNEYSNTPNLDLTTCVKVGDKMETKVLKVNDGDGQVLLTYKRLAIDKGNKRLEE
                     AFNNKEVLKAAVAQVLDGGLSLIIEESRVFIPASLVSDSYEKDLSKFLGQEIEFVITE
                     FNPRRRRIIGDRRQLLMAEREKQQKALFEKISVGDVVEGTVKNVTDFGVFVDLGGADG
                     LLHISEMSWGRVENPRKLFKPGQVVKVLIKDINGTKIALSMKFPETNPWIDADKKYAV
                     GTVITGKVARMTDFGAFVEMEPGIDALLHVSQISREHIEKPSDVLKTGQEVTAKVVDF
                     NLEDKKISLSIKALLEQEAKAEAEAAEKEPEEDEDFVNVDIEAEIQKAEEEE"
     gene            complement(2214..>2924)
                     /gene="ispH"
                     /locus_tag="PLU43_04845"
     CDS             complement(2214..>2924)
                     /gene="ispH"
                     /locus_tag="PLU43_04845"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOO27768.1"
                     /translation="EQRGVQVLHSEEDLRQLESGTVIIRSHGVPKRIYSIIEEKGLNC
                     VDATCPFVKKIHKIVRQESENGVQIIVIGNDIHPEVEGIKGWCDEKLPAIVIETKEEA
                     AAFVCPPKQKLCLVSQTTFNYNKFQELVEFFQKKGYDINVVNTICNATEERQAEAKEI
                     AAAADAMIVIGGMHSSNTQKLYEICRRECKNTQYIQTLDDLVLDSTESISRVGITAGA
                     STPNKIIEEVQNYVRINF"
BASE COUNT          790 a          730 c          449 g          955 t
ORIGIN      
        1 ggattattat tatctgccgg aagaagactg ctgcatccat aaaagcgctg ccatggcggt
       61 cgataaagac caccgcttac atgcaaaaaa agagacctgc tatacaaagc ataccggtct
      121 ctttcttccg caattttcat ctataattac tccatacttc caaacagaat acaaaacaaa
      181 aatactttat tttgaatata acagggacaa tatgacgcct gccgccatga atcaatacgg
      241 aacacatctg ctgaattttt tctgcggata aatgatctta taaattaatt atgctttttc
      301 tgataataga acgaatttct gcgttcataa ccaaaatctc tgtgattcat aatctgttcc
      361 gtacttccaa taaacaaaat accgtcagga acaagcgcat ccgaaaattt ttggaatact
      421 tcatcctttg cctcttccgt aaaataaatc agtacattcc ggcaaacgat aaaatgacat
      481 ctgtccggat aatgatcttt tagcagatta tgttcccgaa actccactct cgatttgatc
      541 tcattcgaaa tctggtaaga ggctccgatc tgtttaaaat atttggatct taaatcagca
      601 ggaacatttg caatgctctt ttccgcgtac aaacctattt tagcctgtgc gatcacctgc
      661 ttatccaggt cagttgcaag aattttgatc tgattaagcg gaagatgcct cgaaagagcc
      721 atcacaagcg aatatggctc atcacctgtc gaacaggccg cactccatat ttttaaattt
      781 ttcccaaact gacgaagcat ttccggaata aacgtcttgt ccagcagttc ccactgatcc
      841 gtattccgat aaaattccga aacattgatc gtcaaataat ttacaaattc ctcaaacagc
      901 tgcttatcag atctgattct gctgataaaa tcttcataac cagaaatcct atgcttattt
      961 attaaagtat caatccttcg tctcatctgc ttttctttgt aggaacttaa atctattcct
     1021 gtcatccgaa gtattgctgc cttaaatccc tcataatcat aagccataaa gtttattcct
     1081 cttcttctgc cttctggatt tcagcttcaa tatccacatt tacaaaatct tcgtcttctt
     1141 ccggctcttt ttcagcagcc tccgcctctg cttttgcttc ctgctccagc agcgctttaa
     1201 tacttaaact gattttttta tcttcaaggt taaaatcaac aacctttgcc gttacttcct
     1261 gccctgtttt caatacatct gacggttttt caatatgttc ccttgaaatc tgggacacat
     1321 gcagcagggc atcaattccc ggctccattt caacaaatgc accaaaatca gtcattctgg
     1381 caacttttcc cgtaataacc gtaccgaccg catacttttt atcagcatca atccacggat
     1441 tagtctccgg gaatttcata ctcagggcaa tttttgtgcc gttaatatct ttgatcaata
     1501 cttttacgac ctgtccgggc ttaaataatt ttcttggatt ttccacacgt ccccatgaca
     1561 tctcagaaat atgcaataat ccgtctgccc cgccaagatc aacaaacaca ccaaaatctg
     1621 tcacattctt tacggtgcct tctaccacat ctcctacgga gattttttca aacaatgctt
     1681 tctgctgttt ttctctttca gccatcagca gctgtcttct gtctccgatg atccttctcc
     1741 tgcgcggatt gaactcggta attacaaact cgatctcctg tccgaggaat ttggacagat
     1801 ctttttcata actatccgat acaagactgg caggaataaa cacgcgggat tcttcaatga
     1861 tcagactcag accgccgtca agcacctgtg caaccgcagc tttcaatact tctttattat
     1921 taaaggcttc ttccagtctc ttatttccct tatcaattgc aaggcgctta tatgtcagaa
     1981 gtacctgtcc gtctccgtca tttaccttca gcacctttgt ttccatttta tcgcccactt
     2041 ttacacaggt agtaagatcc agattcggcg tattggaata ctcgttcttc gttacaattc
     2101 cgtctgattt gtaaccgata tttaaaataa tctcatccgg cttcacatct atgactgtac
     2161 cttcaactac ctctccattg tgtattgttt taaatgattg ttctaacatc tgttcaaaag
     2221 ttaattctga cataattttg aacctcctca attattttgt ttggggttga tgcccctgca
     2281 gtaatgccta cacgcgagat ggattcggtt gaatctaata ctaagtcatc aagtgtctgt
     2341 atatattgcg tattcttgca ttcacgcctg catatctcat atagcttctg tgtattggaa
     2401 ctatgcattc ctccaatcac aatcatcgca tctgccgcag ccgcaatctc tttcgcttct
     2461 gcctgccgtt cttccgtagc attgcaaatt gtattcacaa catttatatc ataacctttt
     2521 ttttgaaaaa attcaactaa ttcttgaaat ttattatagt taaatgttgt ctgcgaaaca
     2581 aggcaaagct tctgtttcgg gggacaaacg aaagcagcgg cctcctcttt cgtttcaatt
     2641 acgattgccg gcagcttctc atcacaccag cctttgatcc cttctacctc cggatgtata
     2701 tcattcccga taacaatgat ctgcactccg ttttcacttt cctgccgaac aattttatgt
     2761 atttttttta caaacgggca ggtcgcatca acacagttca aacccttttc ttcaatgata
     2821 ctgtaaatcc gtttcggaac cccgtgggaa cggatgatca cagtcccgct ttcaagctgt
     2881 cttaagtctt cctctgaatg gagcacctgt acaccccttt gttc
//
LOCUS       DAOSYE010000223         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25376_length_2915_cov_2.856993, whole genome shotgun sequence.
ACCESSION   DAOSYE010000223 DAOSYE010000000
VERSION     DAOSYE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25376_length_2915_cov_2.856993"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="der"
                     /locus_tag="PLU43_04850"
     CDS             complement(<1..1075)
                     /gene="der"
                     /locus_tag="PLU43_04850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOO27769.1"
                     /translation="MSKPIVAIVGRPNVGKSKLFNVLAGKGISIVKDTPGITRDRIYA
                     DVTWLDRDFTIIDTGGIEPESKDIILSQMREQAQIAIDNADVIIFMADVRQGLQDADS
                     KVADMLRRSKKPIILVVNKVDNFDKYGNDVYEFYNLGIGEPHPISAANMLGIGDMLDE
                     VVLHFADHDALEEEDERPRIALIGKPNVGKSSILNRLLGENRVIVSNVPGTTRDAVDT
                     PVLHNGREYVFIDTAGLRRKNKVKEQLEHYMVVRTVSAVERADIVVLVIDAAEGVTEQ
                     EAKIAGIAHESGKGVIIAVNKWDLIEKNDKTIYQFEKKIRNVLSYMPYAEIIFISALT
                     GQRLNKLFDLIDAVVENHAMRIQT"
     gene            complement(1068..2429)
                     /locus_tag="PLU43_04855"
     CDS             complement(1068..2429)
                     /locus_tag="PLU43_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOO27770.1"
                     /translation="MEKQHIIAQVLAGSIAEELDIQAGDILLKIDGEQMEDIFDYQFF
                     VEEEYLEVLIRKQNGEEWLLEIDKDADEDLGLVFENGLMDEYRSCRNQCIFCFIDQMP
                     PGMRKTLYFKDDDSRLSFLQGNYVTLTNMSDGDIDRIIKYHLSPINISFQTTNPKLRC
                     MMLNNRFAGEALLKVDRLYEAGITMNGQIVLCKGVNDGAELERSISDLIKYMPCLESV
                     SVVPVGLSKFRDGLYPLEPFTREDAVSVLELIQKWQEEAYQAFGMHFVHASDEFYLLA
                     GKELPQAENYDGYLQLENGVGMLRLLLNEYEEAYLSLTDRLAHLSEEKKKRMAEEELE
                     ISLVTGKLAYPAISRMAERIEMLFPKLTVMVYSIRNDFFGERITVAGLLTGQDMIRQL
                     KGKPLGKKLLLPVCTLKSGEQVFLDNVTVLDMEKALQVKIDIVKSSGQDFVNALVGDL
                     IYE"
     gene            complement(2433..2843)
                     /locus_tag="PLU43_04860"
     CDS             complement(2433..2843)
                     /locus_tag="PLU43_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO27771.1"
                     /translation="MNILFFLTPKSEVEFVYNDDTISSVMEILEKSTYTAIPVISRTG
                     QYRGSVSEGDILWAVKNDYNFDETGFSKVHLSKITKKIKYSPVKVDEKVDNMIDRVIN
                     QNFVPVIDDDEVFIGIVKRKDVLQYYIGQAKKEV"
BASE COUNT          792 a          776 c          478 g          869 t
ORIGIN      
        1 ctgtctgaat acgcatcgca tggttttcca caactgcgtc gatcagatcg aacagcttgt
       61 tcagacgctg tccggtaagt gccgatataa atatgatctc cgcatacggc atataggaaa
      121 gcacattacg gatctttttt tcaaactgat agatcgtctt atcatttttt tcaataagat
      181 cccatttatt gaccgcaatg ataacaccct tcccgctttc atgcgcaatg ccggcaattt
      241 tagcctcctg ctcggtcaca ccttccgctg catcgatcac aagtacgaca atatccgccc
      301 gctccactgc actgaccgta cgtacaacca tataatgctc cagctgttct ttcactttgt
      361 ttttacgccg cagacctgcc gtatcaataa acacatactc cctgccgtta tgcaggacag
      421 gcgtgtcaac ggcatctctc gttgtacccg gcacatttga tacgatcaca cggttttcac
      481 caagtaatct gttcagaatc gaggattttc ccacgttagg ttttcctatg agcgcaatac
      541 gcggccgttc atcctcttct tccagcgcat catgatccgc aaaatgtaaa acaacttcat
      601 caagcatatc accgatacca agcatattgg ccgctgaaat cggatgcggc tcccctatgc
      661 ccagattata aaattcatac acatcattac cgtatttatc aaaattatcc actttattta
      721 caacaagtat gatcggtttt tttgatctgc gcagcatatc ggccactttg gaatcagcat
      781 cctgcagccc ctgtctgaca tctgccataa aaataataac atcggcatta tcaatggcta
      841 tttgcgcctg ctctctcatc tgagataaaa taatatcctt gctttccggc tcgattccac
      901 cggtatcaat aattgtaaaa tctctgtcaa gccatgtaac atctgcataa attctatccc
      961 gtgtaatacc aggcgtatct tttacaatac taatcccctt cccggcaagt acattgaata
     1021 attttgattt ccctacattt ggtctgccga caatggctac tattggttta ctcataaatc
     1081 aaatctccta ctaaggcatt cacaaaatcc tgtccgcttg attttacaat atctattttg
     1141 acttgtaaag ccttttccat atccaaaact gtcacgttat caagaaacac ctgttctccg
     1201 ctcttcagcg tacagaccgg taacagaagc tttttaccaa gcggtttccc cttcagctgt
     1261 ctgatcatat cctgcccggt caaaagcccg gcaacagtga tccgctcccc aaaaaaatca
     1321 tttcgaatac tatataccat aacggtcagt tttggaaaaa gcatttcaat acgttctgcc
     1381 atccggctga tcgccggata agcaagtttc cccgttacaa gggaaatctc caattcttct
     1441 tccgccattc tttttttctt ttcttccgac agatgtgcaa gcctgtccgt aagcgacagg
     1501 tatgcttctt catattcatt cagaagcagt ctaagcatcc caacgccgtt ttccagctgc
     1561 aaatatccat cataattttc cgcctgcggc agttctttgc ctgcaagcag gtaaaattca
     1621 tccgatgcat gaacaaaatg catgccgaaa gcctggtatg cttcttcctg ccatttctga
     1681 atcagttcca gcaccgaaac cgcatcttcc cttgtaaatg gttcaagcgg atacagtcca
     1741 tccctgaatt tcgataatcc gaccggaacg accgatacac tttcaagaca cggcatatat
     1801 ttaatcagat ccgaaatact gcgctccaat tccgcaccat cgttcactcc tttgcagaga
     1861 acgatctgtc cgttcatcgt aataccggcc tcatacagcc ggtcaacttt caaaagcgcc
     1921 tcaccggcaa accggttatt cagcatcatg cagcgcagct ttgggttcgt cgtctgaaat
     1981 gaaatattga tcggtgacaa atgatatttg ataatgcggt caatatcccc gtcactcata
     2041 tttgtcagcg tcacataatt tccctgtaaa aaagaaagcc tggaatcatc atctttaaaa
     2101 taaagtgttt tgcgcattcc aggaggcatt tgatcaataa aacaaaaaat acactgattt
     2161 ctgcaggagc gatattcgtc catcaggcca ttttcaaaaa caagtcccag atcttcatct
     2221 gcatccttat caatttccag aagccattct tccccattct gttttctgat cagcacctct
     2281 aaatattctt cttcaacaaa aaactgataa tcaaaaatat cttccatctg ttctccgtcg
     2341 atcttcagaa gaatatcacc cgcctgaata tccaattctt cggcaatact gcctgcgagc
     2401 acctgagcga ttatatgctg cttttccatg aattaaactt ccttctttgc ctgaccgata
     2461 taatactgca agacatcctt tcttttcacg attccgataa atacttcgtc atcatcaatg
     2521 acgggcacaa aattctggtt gatgacccga tcaatcatat tgtccacctt ctcgtcaacc
     2581 ttaaccggac tgtattttat cttttttgtg attttagata aatgaacttt tgaaaaaccg
     2641 gtttcatcaa aattgtagtc atttttgacc gcccagagaa tatcgccctc ggacacgctc
     2701 ccgcgatact gcccggttct gctgatcaca gggatcgcag tatatgtgct tttctcaaga
     2761 atctccatta cgctggaaat cgtatcgtca ttatatacaa attccacttc gcttttgggt
     2821 gtcagaaaaa ataaaatatt catacttgat tttcctccgg cgcatcaata gctagtgctg
     2881 ccaaacccgc cgttgcggat ctcttcgaca tcgtc
//
LOCUS       DAOSYE010000224         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25413_length_2912_cov_3.044802, whole genome shotgun sequence.
ACCESSION   DAOSYE010000224 DAOSYE010000000
VERSION     DAOSYE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25413_length_2912_cov_3.044802"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PLU43_04865"
     CDS             complement(<1..103)
                     /locus_tag="PLU43_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOO27772.1"
                     /translation="MNGDRAAWIKSGQKLIHKAKFVLDCFYMHKYIIG"
     gene            complement(234..911)
                     /locus_tag="PLU43_04870"
     CDS             complement(234..911)
                     /locus_tag="PLU43_04870"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOO27773.1"
                     /translation="MEEKKEFTMPLIGDKAPSFTANTTQGEIKFPEDYAGKWVILFSH
                     PADFTPVCTTEFMTFASMINEFRELNTELIGLSVDSIYSHIAWLRKIQEISYKGMKDV
                     EVTFPLIEDIKMEVANKFGMIQPGQSTTQAVRAVFVIDPEANIRCILYYPLTTGRNFD
                     EIKRVILALQKADKDQVATPADWRPGDDVIVPTAGSCGTAKERMENKAEDTYCLDWFM
                     CFKKEQK"
     gene            complement(1020..1454)
                     /locus_tag="PLU43_04875"
     CDS             complement(1020..1454)
                     /locus_tag="PLU43_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO27774.1"
                     /translation="MEKNELSNALGIIEERFGHDTLISLATLDGTIPSVRIVNSYYEN
                     GAFYTVTYALSNKMKQIKANHTVALCGEWFTAHGIGENMGHVGSEQNKEIADKLRQVF
                     SAWYSNGHVDESDPNTVILCVRLTDGVLFSNGTRYEIDFNSI"
     gene            complement(1441..2178)
                     /locus_tag="PLU43_04880"
     CDS             complement(1441..2178)
                     /locus_tag="PLU43_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO27775.1"
                     /translation="MRKEELLYENSSTFIKQLLFSLGVDLCGIACIERFQGAPKGYHP
                     TDILPSCRSVISFGCRFPVGTLICSSNIPYTRVRNSITSKMDSIALDFCIEMEKHQIL
                     CIPIPVNESVWDENTGRWRSIVSLKHAAQAAGLGTIGRHSLLITPEFGSMIWLGAVLC
                     ESKLEPDDLKENICDHCNLCVNECPVNALEDEELRQQTCWDYAFGENEHTKVWQISCH
                     KCRDVCPYNLGTENKKLLEKGDQHGKE"
     gene            2405..>2912
                     /locus_tag="PLU43_04885"
     CDS             2405..>2912
                     /locus_tag="PLU43_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOO27776.1"
                     /translation="MSYVDKAIDGEEAVCPGSDYPIHKKVLSTFQMLMQNEKRMSIAA
                     NEILNVASSISSFDVEMAHISKQLMDFAGEMGDLSESNLAIVEETNASMNQVTETIDT
                     TADTLSMLSNQSNGLADKNNKSIQLLNEVIDIKENVIANTRNMNDKIEQLTELANEVS
                     KIVESVQAI"
BASE COUNT          833 a          668 c          523 g          888 t
ORIGIN      
        1 ctcctatgat atatttgtgc atataaaaac agtcaaggac aaactttgct ttatggatta
       61 gtttctgccc gcttttaatc catgcggctc tatctccatt tatatagatc ttttccagat
      121 attatgtacg ttctattttt tttacttatg tgtcacgttc aaaaattttc ataaaaaaag
      181 aatatgccgg tattcattgg cacatccttc tcttcttatc tgttttactg ccgttatttc
      241 tgttcctttt taaagcacat aaaccagtca agacaatagg tgtcttccgc tttattctcc
      301 attctctcct ttgccgttcc gcaggaaccg gcagtcggaa cgatcacatc atcccccggt
      361 ctccagtccg caggtgttgc cacctgatcc ttgtccgctt tctgcagtgc cagaataact
      421 cgtttgattt catcaaaatt tctgcccgtt gtaagcggat aatacagaat acaccggata
      481 ttcgcttccg gatctatgac aaacacggct ctgaccgcct gcgttgttga ctgtccgggc
      541 tgtatcatcc caaacttatt tgcaacttcc attttgatat cttcgatcag cggaaacgta
      601 acttcaacat ctttcatccc tttatagctg atctcctgta ttttacgaag ccatgctata
      661 tgcgaatata tggagtccac agacaatcct atcagttccg tattcaattc cctgaattca
      721 tttatcattg atgcaaacgt cataaattcg gtcgtgcaga caggtgtaaa gtctgccgga
      781 tgggaaaaaa ggatcaccca tttccctgca taatcttcag ggaattttat ttctccctgt
      841 gtcgtattgg ctgtaaaaga cggcgcttta tctccgatca gcggcattgt gaattctttc
      901 ttttcttcca taaaacagta cctccttgca tttgaacaga atcatacata ataaaagcta
      961 ttgattagtt gcaactaact ctctcgctca ttatacctat aaccccgtca tctgtcaatt
     1021 caaatggaat taaaatcaat ctcatacctt gtcccgttgc tgaaaaggac cccatctgtc
     1081 agccggacac acagaatgac cgtattggga tcgctttcat cgacatgtcc gtttgaatac
     1141 catgcagaaa atacctgccg cagcttgtct gcgatctctt tattctgttc gctgcctaca
     1201 tgccccatgt tctcgccgat cccatgtgca gtgaaccatt ccccgcacaa tgcgaccgta
     1261 tggtttgctt tgatctgttt cattttattg gagagtgcat aagtaaccgt ataaaacgct
     1321 ccgttttcat aataactgtt tacgatcctg acagacggaa tcgttccgtc caatgtggca
     1381 agcgaaataa gtgtatcatg tccaaatcgt tcctcaatga tgccaagtgc atttgaaagc
     1441 tcattctttt ccatgctgat ctcctttttc caataacttt ttattttcag ttcccagatt
     1501 atatggacag acatctctgc atttatgaca ggatatctgc cagacctttg tatgctcatt
     1561 ttccccgaat gcataatccc agcaggtctg ctgcctaagt tcctcatctt ccagtgcatt
     1621 tacaggacat tcgtttacac ataaattaca atgatcacaa atattttctt tcagatcatc
     1681 cggctccagt ttgctttcac ataacacggc accaagccag atcatacttc cgaattcagg
     1741 tgtgatcaga agagaatgcc tgccgatcgt cccaagaccg gcagcctgtg ccgcatgctt
     1801 cagggaaaca atggaacgcc accgtcctgt attttcatcc catacgcttt catttacagg
     1861 gatcggtatg cataatatct ggtgtttttc catctcaata caaaaatcca gcgcgatcga
     1921 atccatttta gacgtgatgg aatttctcac acgcgtgtag ggaatgtttg aactgcatat
     1981 cagtgttcca accgggaatc tgcatccaaa tgaaatcact gatctgcatg acggcagaat
     2041 atctgtcgga tgatatcctt ttggcgcacc ctggaatctt tcaatacatg caataccgca
     2101 taaatccacg ccgagtgaaa acagcagctg tttaataaat gtactgctgt tttcataaag
     2161 taactcttct tttctcatat aagcctccgc agtcattttt tttattataa accacagacg
     2221 tgacaactat atgtcatatt tacataaaaa tatcaccaca aatatctgtt tttttttaaa
     2281 aaaaaatgtt acactattaa agtaaactat taaacaaacc gtattgtatg agactaataa
     2341 taaaaactgg aggaaaaata tgggattgaa taaaaaaggt agttgctcgg aaatgagctg
     2401 tattatgagt tatgttgata aggcaattga tggagaagaa gcggtctgtc ccggcagtga
     2461 ttacccgatt cacaaaaagg tcctctcgac ttttcagatg ctgatgcaga acgaaaaacg
     2521 aatgtccatt gctgccaatg agatcctgaa tgttgccagc tctatcagtt cctttgatgt
     2581 tgaaatggcg catatttcaa aacaattaat ggattttgca ggagaaatgg gtgatctgag
     2641 cgagtccaat ctcgccattg ttgaagagac gaacgcttcc atgaaccagg tgacagaaac
     2701 gatcgataca acagcagata cactttctat gctttccaat cagtcaaacg gactcgctga
     2761 taaaaataat aaaagtatcc agctgttaaa cgaagttatc gatattaagg aaaacgtcat
     2821 tgcaaacacc cggaatatga atgataaaat cgaacagctg acagaactcg ccaatgaagt
     2881 aagcaagatc gtggaaagcg tgcaggcaat tg
//
LOCUS       DAOSYE010000225         2910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25434_length_2910_cov_3.433975, whole genome shotgun sequence.
ACCESSION   DAOSYE010000225 DAOSYE010000000
VERSION     DAOSYE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_2910_cov_3.433975"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PLU43_04890"
     CDS             <1..154
                     /locus_tag="PLU43_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HOO27777.1"
                     /translation="SPVGHLRCEDRVWQIRDGGIGPVSQKLYDTVTGIQIGNIEGPKG
                     WVVEVK"
     gene            305..1447
                     /locus_tag="PLU43_04895"
     CDS             305..1447
                     /locus_tag="PLU43_04895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO27778.1"
                     /translation="MKKLTLTRDMLVTGENSLDYLAEIKCKKAVIITGGQSMKKTGVL
                     DKVTAIMEGNSVEVSIYSGVGKNPTTKMVLDGLDYVKQKQPDCILAVGGGSSIDAAKV
                     MLIFYEHPEINFDNIFDVNLDELCFTTKFIAVPSTSGTASEVTKVSVITFEEEQFKRA
                     IRTINIFPNIAILDPSLPVTLPSHIAAETGMDALTHALEAYINKNGNDFTDVLAKGAI
                     EGIIEWLPESVIEGSPAAREKIHNYQCMAGMAFANSGLGMVHGVSHAFGGMYNVAHGL
                     ANAVILPYSMDYNKKDPDVFKKYEILSRALGNDVIGKVKELSARIGIPANMQDAGVSE
                     EDFKKDFDKLLGFSMKGSTVVNPIPVSKEDMKKFLDYIYYGKKIDF"
     gene            complement(1583..>2910)
                     /locus_tag="PLU43_04900"
     CDS             complement(1583..>2910)
                     /locus_tag="PLU43_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOO27779.1"
                     /translation="KYGLASTWIGLGNAAIGSLLAWIVLGRRTRVMTNHLTSATMPEF
                     FGKRFESHSLKLAASLIIFVFLVPYTASIYNGLSRLFEMAFHIPYAVCIIVMAFLTGV
                     YVIAGGYMATAINDFIQGIIMLVGICAVIAAVLNGQGGFIEAVGKLAQFESDIEPTLG
                     QPGAFTSFFGTDPVNLISIIILTSLGTWGLPQMVHKFYAIKDEASVKSGTVISTIFAV
                     VVAGGSYFLGAFGRLFDTPGLHKEDGSVIYDAIMPYMLSTLPDILVGIVIVLVLSASM
                     STLSSLVLTSSSTLTLDFINGTLIKKMDEKKQLLTMRILVAFFIILSVILALNPPTFI
                     AQLMGISWGALAGAFLGPFIYALYWKKATKASIWASFIFGVGITVLNLFVKLFSSPIT
                     AGAFAMLASLVIVPLVSLFTPKLSSRSIDSIFECYTETVIVSKKKSLQE"
BASE COUNT          893 a          556 c          733 g          728 t
ORIGIN      
        1 ctccccggtc ggacatttaa gatgcgaaga cagggtatgg cagattaggg acggcggtat
       61 cggacctgtc agtcagaaac tttatgatac tgttacgggt atccagatcg gaaatatcga
      121 aggtccaaag ggctgggtag tcgaagtgaa ataatttgta tcagcagcat tagaagcaat
      181 cctttgattt tggggcaaag gattgctttt tgatattctg attagaaaag gtctattttt
      241 tacaatttct ttttaaaaaa agtgtttata gtaaagataa aaaataaaac aggaggcgat
      301 gaaagtgaaa aaactgactc tgacaaggga catgcttgtt accggagaaa attcattgga
      361 ttatctggca gagatcaagt gcaaaaaagc ggtgattatt acaggcggcc agtcaatgaa
      421 aaaaactggc gtattggata aagtaacggc gatcatggaa ggaaatagcg tggaagtatc
      481 catttattca ggagttggta aaaatcctac gacaaaaatg gttttagatg gtctggacta
      541 tgtgaaacaa aagcagccgg actgcatatt ggcagttggc ggaggctctt ccattgatgc
      601 tgcaaaggta atgctgattt tctatgagca tccggaaatc aattttgaca atatatttga
      661 tgtgaatctg gatgaactgt gctttacgac aaaatttata gcagtgccgt ctacttcagg
      721 aacagcttca gaggttacta aggtcagtgt gatcacgttt gaggaagagc agtttaagag
      781 agcgatcaga acgatcaata tatttccgaa tatagcaatt ctggatccgt cactgccggt
      841 cacccttccg tcacatattg ccgcggaaac gggaatggat gcgctgacac atgcattgga
      901 ggcttatatc aataagaatg gaaatgattt tacagatgtt cttgccaaag gggccataga
      961 aggaatcata gagtggctgc cggagtctgt tattgaggga agtccggccg ccagggaaaa
     1021 gatacataat tatcaatgca tggccggcat ggcatttgcc aacagcggtc ttggaatggt
     1081 acacggtgtt tcccatgctt tcggcgggat gtataatgtg gctcatggtc tggctaatgc
     1141 ggtgattctg ccgtattcca tggattataa taaaaaggat ccggatgttt ttaaaaagta
     1201 tgaaatactt tcccgtgctt tgggaaatga tgtgatcggg aaagtcaaag aactgtctgc
     1261 caggatcggt attcctgcca atatgcagga tgctggagta tccgaggagg attttaaaaa
     1321 ggattttgat aaactgctgg gattttccat gaaaggatct acggttgtga atccgattcc
     1381 ggtttcaaaa gaagatatga aaaagtttct tgattatatt tattatggca aaaaaataga
     1441 cttttaggag ttcaagacct atacttccat gacaccaaga caatattgca ggaaagtagg
     1501 aatggatatt gtttgacagg taaaaaaata tgtccgggat cccgatgaaa aattgggatc
     1561 ccggacatat ttgagcagca gtttattcct gcagcgattt cttttttgat acgattacgg
     1621 tttccgtata acattcaaaa atcgaatcaa tggaacggct gctgagcttt ggcgtaaaca
     1681 ggcttacgag cggtacaata acaagagaag ccagcatcgc gaaagcgcct gccgtgatcg
     1741 gcgaagaaaa gagctttaca aacaggttca gcacggtaat gccgactccg aaaataaagc
     1801 tggcccatat ggaagccttt gtagcctttt tccagtaaag tgcgtaaata aacggtccga
     1861 ggaacgcgcc ggccagtgcg ccccaggaaa ttcccatgag ctgcgcgata aaggtgggcg
     1921 gattcaatgc cagaatgaca gaaagtataa taaagaatgc aacgaggatc cgcattgtta
     1981 aaagctgttt tttctcgtcc atttttttaa taagagtgcc gtttataaaa tccagtgtca
     2041 gtgtggacga tgatgtcagt acgagtgaag acagcgtgga cattgaagcg gaaagcacaa
     2101 gtacgataac gatgcctacg agaatgtcgg gaagcgttga gagcatgtac ggcatgatgg
     2161 catcataaat aacactgccg tcttccttat gaagcccggg ggtgtcaaaa agcctgccga
     2221 aagcgccgag aaaataggag ccgccggcaa caacgactgc aaatatggta gagatgactg
     2281 ttccggactt tacggaagcc tcatccttga tcgcataaaa tttatgaacc atctgcggaa
     2341 gtccccatgt accgagtgaa gtcagaatga tgatgctgat cagattgaca ggatccgtac
     2401 cgaagaaaga agtaaaggcg ccgggctgtc cgagtgttgg ttcgatatca ctttcaaact
     2461 gcgcgagctt tccgacagct tcaataaaac cgccctgccc gttcagtacg gcagctatga
     2521 ccgcgcagat accgaccagc ataataatgc cctgaatgaa gtcattgatc gcggtagcca
     2581 tgtaaccgcc ggcaattaca taaacgcctg tcagaaaagc cattacgatg atgcagacag
     2641 cgtaggggat atggaaagcc atttcaaaca gccttgaaag accgttgtag atggaagccg
     2701 tataaggtac caaaaaaaca aaaataatta aagaggctgc cagtttcagg gaatgacttt
     2761 caaatctttt tccgaaaaat tccggcatcg tggcacttgt caggtgattt gtcatgaccc
     2821 gggtgcgtcg tccgaggacg atccatgcga gcagacttcc gatcgctgca ttcccaagac
     2881 cgatccaggt ggaagccagt ccgtatttcc
//
LOCUS       DAOSYE010000226         2902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25556_length_2902_cov_3.019319, whole genome shotgun sequence.
ACCESSION   DAOSYE010000226 DAOSYE010000000
VERSION     DAOSYE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25556_length_2902_cov_3.019319"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLU43_04905"
     CDS             complement(<1..690)
                     /locus_tag="PLU43_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOO27780.1"
                     /translation="MIQISQIKLPVSKNEQELADKIIQILQLKRIYHNRTFPDFTYKI
                     IRRSLDARKKPALFFVYSVAVYFSEKEEPAIMRQNRNENIILTEDLHYVPPKHGSRSL
                     GKNPVIVGSGPAGLFCAYLLAHEGYCPIVLERGENVDNRVNSVEKFWAGEAVNPESNV
                     QFGEGGAGTFSDGKLNTLIKDRDGRNHYVLDTFVRHGAPKECAYIAKPHVGTDKLIKI
                     VKSMRKEIIDLG"
     gene            complement(687..1895)
                     /locus_tag="PLU43_04910"
     CDS             complement(687..1895)
                     /locus_tag="PLU43_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO27781.1"
                     /translation="MKIGIIGGGAAGMTAAVTASEMGHNVTILEHENRIGKKILLTGN
                     GRCNLTNTDQALFHYHGQDPEFAAAVFKQCSYTDALSFFTRLGIYTKNKNGCLYPYSE
                     QASAVLDVLRIALKNRGVRVITECNIQSVKKTDCFSITTDKEPYKFDKVIIAAGSKAA
                     AQTGSDGSGYQLAKELGHHLIKPLPALVQLRSKEPYFKQLAGIRTTASLDILCDGEYT
                     GTEYGELQLTNYGLSGIPIFQLSHIAAKALDLGQEVAVCIDFMPDMDSFHDICAYLQT
                     RIRSCPEKTAEELLIGVLHKKLGQLLIRLCNIRPDCLVKNLTEKDVERISGLIKRFTV
                     PITSTNSFKEAQVCSGGVDTAELKTTLESKLVDGLYFAGEIVDIHGDCGGYNLQWAWS
                     SGIVAGRILS"
     gene            complement(2062..>2902)
                     /locus_tag="PLU43_04915"
     CDS             complement(2062..>2902)
                     /locus_tag="PLU43_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOO27782.1"
                     /translation="SDTLDQSIFNYIMTAKGLESQKAALWGIFSTKYKVLTNVPIALA
                     SAVCSSMMPSLTACVEQNELRMARHKVSTATRFTMILAIPCAVGLSVLGKPIISMLFT
                     GEVDMPAQLLRVGSISVVFYSLSTLSNGVLQGIDKLRIPVRNAAIALVIHLGILYLTL
                     HVFDWMLYGVVFSCIMFAFIMCVLNWLSIKKYLKYRQEVKRTFFIPLVASGIMAFFVW
                     LINFLLSKTASNIVAVLVSIFAGALIYFIILVLLRGVKESEIRSFPGGDLLAGTARLF
                     HLL"
BASE COUNT          798 a          701 c          578 g          825 t
ORIGIN      
        1 accaaggtct atgatctctt ttcgcatgct ttttacaatt ttgatcagtt tatctgttcc
       61 gacgtgcggt ttagcaatat atgcgcattc ttttggagct ccatgcctta caaacgtgtc
      121 cagaacatag tgatttcgac cgtcccggtc tttaataagc gtgtttaatt taccatcgga
      181 aaatgttccc gcaccgcctt cgccaaattg tacatttgat tccggattga ctgcttctcc
      241 agcccaaaac ttttccactg aattcacacg attgtccaca ttttctcccc gttcaagcac
      301 aattgggcag tatccttcgt gagccaataa atatgcacag aacaaaccgg cgggaccgct
      361 gccgacaata acaggatttt ttccaagtga tcggctgcca tgctttggcg gcacatagtg
      421 cagatcttct gtcaatataa tattttcatt tctattctgc cgcataatag ccggttcttc
      481 tttctctgaa aaataaacgg caacagaata aacgaagaac aatgccggct tctttcttgc
      541 atcaagcgaa cggcgtatga ttttatatgt aaaatcagga aatgttctgt tatggtaaat
      601 acgctttaac tgcagtatct gtattatttt gtccgcaagc tcctgttcgt tttttgaaac
      661 cggcagcttg atctggctga tttgaatcat gacaatatcc ttcccgccac gataccgctt
      721 gaccatgccc attgcaggtt atagccgccg cagtcaccgt ggatatcgac gatttctccg
      781 gcaaaataga gaccgtctac aagctttgat tccaaagtcg tcttcaattc tgccgtatcc
      841 acaccgccgc tgcacacctg tgcttcttta aaagaatttg tcgatgtgat cggtacggtg
      901 aatcttttta taaggccgga gatcctttcc acgtcttttt ctgtaagatt tttgacaaga
      961 cagtctggtc tgatattaca aagcctgatc agaagctgtc ccagcttttt atgcagcaca
     1021 ccaataagca gctcttctgc cgtcttttcc ggacatgacc tgatcctggt ttgtaaatat
     1081 gcacatatat catgaaagga gtccatatca ggcataaaat cgatacatac agcaacttcc
     1141 tgtccaagat ccagcgcttt cgcagcaata tggctcagtt gaaatatcgg aatgccggaa
     1201 agcccataat tggtcagctg gagttctcca tattccgttc ctgtatattc accgtcacag
     1261 aggatatcca gtgatgctgt tgtccgtatt ccggcaagct gtttaaaata cggttctttt
     1321 gagcgcagct ggacaagcgc cggaagcggt ttgatcagat gatgtcccag ctcctttgca
     1381 agctgatagc cgctgccgtc ggatcccgtc tgtgccgctg cctttgaacc ggcagcaatg
     1441 atcactttgt caaatttgta cggttccttg tcggtcgtta tggaaaaaca gtctgttttt
     1501 ttaacagact gtatattgca ctccgtaata acccttacac ctctgttttt cagcgcaata
     1561 cgaaggacat ccaatactgc ggacgcctgt tctgagtacg gatacagaca cccgttttta
     1621 ttttttgtat aaataccaag ccttgtaaaa aaagaaagcg catccgtata gctgcattgt
     1681 ttaaatacgg cagccgcaaa ttccggatcc tggccatgat aatgaaaaag cgcctgatcc
     1741 gtattcgtaa ggttacatct gccgtttccg gtcagaagta ttttttttcc gatgcggttt
     1801 tcatgttcga gaatcgtgac attgtgtccc atctccgatg cagtgaccgc cgctgtcatc
     1861 cctgcggctc ctccgccgat aattcctatt ttcatttgca tattccttcc gtccagacag
     1921 caggctaaaa tgaatgcctg ctctaatcat acgcatttta aacaatattt tcaaccatga
     1981 aataaaagct gaacagcagg tatttccata gagcagttgc ctgtcggatc agcttttatg
     2041 ataggatgca tttttattat tttacagcag atggaaaagc cttgcggtcc cggctaacag
     2101 atcaccgcca ggaaagcttc tgatttcgga ttcttttact ccgcgcaata atacaagtat
     2161 gataaaataa atcagtgccc cggcaaaaat cgataccagt acggcaacga tatttgaagc
     2221 agttttagaa agcaggaaat tgatcagcca tacaaaaaat gccataatac cggacgcaac
     2281 cagcggaatg aaaaaggtac gcttgacctc ctgccggtat ttcaaatatt tttttatact
     2341 gagccagttc agtacacaca taataaatgc aaacataata caggaaaaaa cgaccccata
     2401 tagcatccag tcaaatacgt gcagcgtcag ataaaggatg ccaagatgga tcacaagagc
     2461 gatcgcagca tttctgacag ggatccgcag cttgtcaatt ccctgtaaaa caccgtttga
     2521 taacgtggat aaggaataga acactaccga gatcgaacca acccgcaata gctgtgccgg
     2581 catatccact tcccctgtaa acagcatgga aatgatcggt tttccaagaa cacttaaacc
     2641 aaccgcacac ggaattgcca gaatcattgt aaatcgcgtt gccgtactga ctttatgtct
     2701 ggccatacgc agttcgttct gttccacaca ggcggtaagt gaaggcatca tggaggaaca
     2761 gacagcactg gcaagcgcga tcggcacatt cgtcaacact ttgtattttg tggaaaaaat
     2821 accccacaaa gctgcttttt gcgactccaa ccctttagct gtcataatat aattaaaaat
     2881 actctgatcc agtgtatcgc tg
//
LOCUS       DAOSYE010000227         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25580_length_2901_cov_2.679199, whole genome shotgun sequence.
ACCESSION   DAOSYE010000227 DAOSYE010000000
VERSION     DAOSYE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25580_length_2901_cov_2.679199"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLU43_04920"
     CDS             <1..588
                     /locus_tag="PLU43_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOO27783.1"
                     /translation="MMESWGVPSLLLHSKAYEYASILAKKGIKVVDMAFAGGLAREDQ
                     IFKAIALGAPFVKSVCMGRAMMIPGFLGSNIEGVLRPDRKEKVNGNWDKLPASVASIG
                     LTAEEIFAGYYDVRKKVGADDMDNIPYGAIAITTLVDKLGCGLQQLMAGARKFSIAEI
                     SRDDIFSGNREIEKETKIPFVTDVQDDTAKQLLNL"
     gene            658..1155
                     /locus_tag="PLU43_04925"
     CDS             658..1155
                     /locus_tag="PLU43_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO27784.1"
                     /translation="MQLSAKVADAMITYFEGDIKRIDHFLKVYAFAKTIGELEEIDGQ
                     TQTILEIAALTHDIGIKNSEKKYNSSAGHYQELEGPGEAWTLLWELQVDENIIERVCW
                     LIAHHHTYEGIDDIDYQILVEADFLVNAAEEHMKKNALESVKTKIFRTEAGKKLFDDI
                     YFRGF"
     gene            1176..2432
                     /locus_tag="PLU43_04930"
     CDS             1176..2432
                     /locus_tag="PLU43_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO27785.1"
                     /translation="MSVMMKLKKTGLYRTAVQIISFIFLPVIYAVTLSDIKTLYLGVT
                     KQTVSASVLLPAGLRVCLILFLTALFGRFFCGWICSFGAVGDWVHAIVGRIHKVRFRM
                     PEEADQILKFVKYGFLIFLLVFLWRSNVPALESASPWGAFAAVFSLNGPDFSALMEYY
                     AAGGVFLLVILIGSVFVERFFCRYVCPLGAVFALISNFKILFIKKEREHCGTCKACTN
                     ACAMGIPLYKTDKVMSGECIMCMKCTQICPRKNAALFIGNTKVNTAIAGTAAFAALTA
                     VSYGSDVLAEEITGTADQDAVITEEVQGQYTDGVYTGTGSGFHGGTTSVSVTVENGNI
                     SNVEVLSYEDDKDFFDKAKSEVISEIIETQSPDVDAVSGATYSSRGIMEAVADALGTE
                     LSESTEETPAEDHSGGNGGAGRHKNH"
     gene            2551..>2901
                     /locus_tag="PLU43_04935"
     CDS             2551..>2901
                     /locus_tag="PLU43_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27786.1"
                     /translation="MMNIGYACRALAVPGSEIKGCMAKNASEERILQLIGQNLAAFKK
                     MVEYNIENGIRLFRISSDIIPFGSSLAANIPWEKLYADRLLEISRLITGAGMRVSMHP
                     GQYTVLNSPSVSVVE"
BASE COUNT          800 a          531 c          794 g          776 t
ORIGIN      
        1 atgatggaat cgtggggtgt accgtctctt ctgcttcatt ccaaagcata tgaatatgct
       61 tcgatccttg caaaaaaagg aataaaagtt gtcgatatgg cgtttgccgg cggactggca
      121 agagaagatc agatctttaa ggcgatcgca ctgggcgctc cttttgtaaa atcagtatgt
      181 atgggaaggg ccatgatgat accgggattt ttaggttcca atatcgaagg agtcctgcgc
      241 ccggacagaa aagaaaaggt aaacggaaac tgggataagc ttccggcgtc ggtcgcgtcg
      301 atcggcctga cagctgaaga gatctttgcc ggatattatg atgtcaggaa aaaggtcgga
      361 gccgacgaca tggacaatat tccatatggt gcgatcgcga tcacaacgct tgttgataag
      421 ctgggctgcg gtctgcagca gctgatggca ggggcaagaa aattttctat agccgagatc
      481 tccagggatg atattttttc aggaaacaga gagatcgaga aagaaacaaa gattcctttt
      541 gttacggatg tgcaggatga cacagccaaa caactattga atctgtaatt tcataggaga
      601 aactccggtt cgtttttggc cggagtttct taatacagta taaaggagga agacaatatg
      661 cagctgtctg caaaggtagc agatgccatg atcacctatt ttgaaggaga tataaaacgg
      721 atcgatcact ttttaaaggt gtatgcattt gcaaaaacga ttggggaact ggaagaaata
      781 gatgggcaga cgcagacgat tttggagatt gctgcgctta cacatgatat cgggatcaaa
      841 aacagtgaaa agaaatacaa cagttcagcg gggcattatc aggagcttga aggaccgggg
      901 gaggcatgga cgctgctctg ggaattacag gtcgatgaaa acataatcga acgggtctgc
      961 tggctgatcg cgcatcatca tacatatgaa ggtattgacg acatagatta tcagatactt
     1021 gtggaggctg attttttagt caatgctgcg gaagagcata tgaaaaaaaa tgcgctggaa
     1081 agcgtgaaga caaaaatatt caggacagag gccggaaaga aactatttga tgatatttat
     1141 ttcaggggat tttaaaggaa ggaaggggga cccgtatgtc ggttatgatg aaactaaaga
     1201 agaccggtct gtatcggact gccgtgcaga tcatcagttt tatatttctt cccgttattt
     1261 atgcggtaac actgtccgat atcaagacac tgtatcttgg cgttacaaag cagacagtct
     1321 ctgcgtccgt attactgccc gcagggctgc gcgtctgcct gatcctgttt ttgactgcac
     1381 tgttcggaag atttttctgc gggtggatct gcagcttcgg agctgttggc gactgggtcc
     1441 atgcgattgt gggcaggatt cataaggtcc gtttccgtat gccggaagag gcggaccaaa
     1501 ttttgaaatt tgtaaagtat ggatttttga tttttctgct tgtattttta tggaggtcga
     1561 atgtgccggc tttggaaagt gcaagtccat ggggagcctt tgccgctgtt ttttcattga
     1621 atggccctga tttttcggct cttatggagt actatgcggc cggcggtgtc tttcttcttg
     1681 tcattctgat cggctccgta tttgtggaac gttttttttg ccggtatgta tgtccgctgg
     1741 gggcggtctt tgcactgatc agcaatttta aaatcctgtt tataaaaaaa gagagagaac
     1801 actgcggtac atgcaaagcc tgtacgaatg cctgtgcaat gggaattcct ctttataaaa
     1861 cggataaggt catgagcggc gaatgtatca tgtgcatgaa gtgtacacag atatgtccga
     1921 gaaagaatgc ggccctcttt attggaaata caaaggtaaa tacagctatt gcagggacag
     1981 ccgcgtttgc tgcgctgaca gctgtctcct atggttctga tgttctggcg gaagaaataa
     2041 cgggtacggc agaccaggat gctgtcataa cggaagaggt acagggacaa tatacggatg
     2101 gagtttatac cggaacgggt tccggatttc acggaggaac gacttcggta tccgtaactg
     2161 tagagaatgg gaatatttcg aatgttgaag tactgtccta tgaagatgac aaagattttt
     2221 ttgataaggc gaaaagcgaa gtgatctcgg aaataatcga aacgcagtca ccggatgtgg
     2281 atgcggtttc aggtgcgaca tacagcagca ggggaatcat ggaagctgtt gcggatgccc
     2341 ttggaacgga attatcggaa agtacggaag agactcccgc agaagatcat tcgggcggga
     2401 acggaggtgc cggcaggcat aaaaatcatt gagtttgttt aaattttcaa aaatttcgga
     2461 agttatgtca aatcccttta cacggtattt tttctatgat aaggtggatt cataagaggg
     2521 aaaacggtat ttgtccggga aggatgacag atgatgaata ttggttatgc ctgccgtgcg
     2581 cttgccgttc ccggcagtga aataaaaggc tgtatggcga agaatgcatc agaggaacgg
     2641 atattgcagc tgatcggaca gaatcttgcc gcatttaaaa aaatggtgga atataatatc
     2701 gagaacggga tccgcctttt tcggatttct tcggatatta taccatttgg ttccagtctg
     2761 gcggcaaata ttccatggga gaagctttat gcggaccgcc ttttagagat cagccgcctg
     2821 atcaccggcg caggcatgcg tgtttccatg catcctggtc agtataccgt attgaattcg
     2881 ccgtcggttt ccgtagttga g
//
LOCUS       DAOSYE010000228         2896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25642_length_2896_cov_3.602253, whole genome shotgun sequence.
ACCESSION   DAOSYE010000228 DAOSYE010000000
VERSION     DAOSYE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25642_length_2896_cov_3.602253"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLU43_04940"
     CDS             <1..320
                     /locus_tag="PLU43_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO27787.1"
                     /translation="EILKQIADLRKETGITIILVSHSMEDVAEYVGRIIVMNQGKIVH
                     DDVPREVFSHYKELEEIGLAAPQVTNILHKLQERGIPVRTGAITIEEAKQEILRVVTP
                     VRH"
     gene            345..1148
                     /locus_tag="PLU43_04945"
     CDS             345..1148
                     /locus_tag="PLU43_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO27788.1"
                     /translation="MIRDITLGQYYPADSILHKLDPRVKLVGTMVFIISLFLFENFTG
                     YIAATVFLIAVIKLSKVPFSFMVKGLKAIVILMLITVVFNLFLTPGEPLFSFWKLTVT
                     KEGLRTAVFMIIRLTYLIIGSSVMTLTTTPSNLTDGMEKLLNPLRVFHVPVHEVAMMM
                     SIALSFIPILLEETDKIMKAQMARGADFESGNLINKAKSLVPLLVPLFVSAFRRANDL
                     AMAMEARCYRGGEGRTKMKPLIYKGRDRAAYGVLLVYFAVIICVRAVLG"
     gene            1179..1916
                     /gene="truA"
                     /locus_tag="PLU43_04950"
     CDS             1179..1916
                     /gene="truA"
                     /locus_tag="PLU43_04950"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO27789.1"
                     /translation="MKRIMLTVAYDGTNYSGWQIQPDKATIEGVLNAELSRLLGEEIR
                     VIGASRTDTGVHANGAIAVFDTNSRIPGEKFSYALNQSLPEDIRIRRSKEVAADFHPR
                     RMKTKKTYEYRIWHDEFPLPTNRLYTHWVYTPLNVEAMRAAAAYFIGEHDFRAFCSAH
                     TQAETTVRTIFDLQIAEQGNLITIRVCGNGFLYHMVRIIAGTLLDIGQGRQEPEAVKG
                     ILLSGDRSMSGPTAPAKGLCLIGYEFK"
     gene            2087..2515
                     /gene="rplM"
                     /locus_tag="PLU43_04955"
     CDS             2087..2515
                     /gene="rplM"
                     /locus_tag="PLU43_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO27790.1"
                     /translation="MKTFMASEAAVERKWYVVDATGYTLGRLASEVAKILRGKNKPIF
                     TPHADTGDYVIIVNAEKIKVTGKKLDQKIYYRHSEYVGGMKETTLREKLAKAPIEVIE
                     SAVEGMLPKGPLGRKMYKKLFVYAGPEHPHAAQQPEELKF"
     gene            2540..>2896
                     /gene="rpsI"
                     /locus_tag="PLU43_04960"
     CDS             2540..>2896
                     /gene="rpsI"
                     /locus_tag="PLU43_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOO27791.1"
                     /translation="MAKASEKFYGTGRRKKSIARVYLVPGKGDITINKRSIDDYFGLE
                     TLKTIVRQPLSATETVDKFDVLVNVHGGGYTGQAGAIRHGIARALLKADVEYRPTLKS
                     AGFLTRDPRMKERKKYG"
BASE COUNT          888 a          548 c          752 g          708 t
ORIGIN      
        1 aggaaatttt gaaacagatt gcagacctgc gtaaagagac cgggatcacg atcatactcg
       61 tatcccacag tatggaagac gttgcggaat atgtcggcag aattattgtg atgaatcagg
      121 gaaagatcgt tcatgatgat gttccccgcg aagtattttc tcattataaa gagctggaag
      181 agatcgggct tgctgcaccg caggtaacca atattctgca taaactgcag gaaagaggaa
      241 ttccggtacg cacaggtgcg atcacgatcg aggaagcaaa gcaggaaata ttaagagtgg
      301 tgacacctgt aagacactga ctgcaggtga taaggtagaa gcagatgatc agagatatta
      361 cattaggaca atattatccg gcagattcta ttcttcataa gctggatccg agagtaaagc
      421 ttgtcggaac gatggtattc attatttctt tatttttatt tgaaaatttt acaggataca
      481 ttgccgcgac cgtttttttg attgcggtca ttaaactgtc aaaagttccg ttttccttta
      541 tggtgaaagg attgaaggcg atcgtaatac tgatgctgat cacggttgtg ttcaatttgt
      601 tcctgacacc gggcgagcct ttgttcagtt tttggaaact gacagtgacc aaggaaggtc
      661 ttcggacggc agtttttatg atcatccgtc tgacgtattt gattatcgga tcatccgtta
      721 tgacgctgac aacgacaccg agcaatctga cagacggtat ggaaaagctg ttaaatccgc
      781 tgcgtgtttt tcatgtaccg gtacatgaag tagcgatgat gatgtcgatc gcattgagct
      841 ttattccgat cctgttggag gaaacggata agatcatgaa agcgcagatg gcacggggag
      901 ctgattttga aagcgggaat ctgatcaaca aggcaaagag ccttgttccc cttcttgttc
      961 cgctgtttgt ttccgccttc cgccgcgcca atgatcttgc aatggccatg gaggcgagat
     1021 gctaccgcgg cggcgaagga aggactaaga tgaagccgct gatctataaa ggaagagaca
     1081 gagctgctta tggggttttg cttgtttatt ttgcagtgat catctgcgtg cgtgcagttc
     1141 ttggataatc ttacgaaatc gaatggcagg gtgatgatat gaagcgcatt atgctgacag
     1201 ttgcatatga cggtaccaat tattcaggct ggcagataca gccggacaag gcaacgattg
     1261 aaggcgtttt aaatgcagag ctttccagac ttttaggaga agagatcagg gtgatcggag
     1321 caagcaggac agataccggc gtccatgcca atggcgccat tgccgtgttt gatacgaatt
     1381 ccagaatacc cggcgagaaa ttttcttatg cgctgaatca gagcctgccg gaagacattc
     1441 gtatccgcag atcaaaagag gtggctgctg attttcatcc gagacggatg aaaacaaaaa
     1501 aaacgtatga atatcgtatc tggcacgatg aatttcctct gccgacaaac aggctttata
     1561 cgcactgggt ctatacacct cttaatgtgg aggccatgcg tgcggcggcc gcatatttta
     1621 tcggagaaca tgatttccgg gcattttgca gtgcacacac gcaggcagag acaacggtca
     1681 gaacaatttt tgatctgcag atagcagagc aggggaatct gataacgatc cgtgtatgcg
     1741 gaaacgggtt tttatatcat atggtaagaa ttattgcagg aaccctgctt gacatcggac
     1801 agggcagaca ggagccggag gcagtcaaag ggatactgct ttcaggtgac cggagcatga
     1861 gcgggccaac agccccggca aagggtttgt gcctgatcgg ctatgaattt aaataaatcg
     1921 agaaaacaat attgacacac aagggtgagt gtaatataat aatttaatgt gacgacaaat
     1981 ggaatgtgca tagccatagc cccggtgaag tacaggaaat atcggcgcaa caatacgtaa
     2041 ctagtaatga aatcagccga agggcttgtt ttgtggaggt atcataatga agacatttat
     2101 ggctagtgag gctgccgttg aaagaaaatg gtatgttgta gacgctacag gatatacatt
     2161 gggacgcctg gcatcagaag tcgccaaaat attaagagga aagaataagc cgatctttac
     2221 accgcatgca gacacaggtg attatgtgat cattgtcaat gcggagaaga tcaaagtaac
     2281 aggcaagaag ctggatcaga agatctatta cagacattca gaatatgtag gcggaatgaa
     2341 agagacgacc ttgagagaaa agcttgcaaa agctccgatc gaggttattg aaagtgctgt
     2401 tgaaggaatg cttcccaaag gaccattagg aagaaaaatg tataaaaaat tattcgttta
     2461 tgcaggacct gagcatcctc atgcagctca gcagcctgaa gaactgaaat tttaatagga
     2521 ccggaaggag gaaattacag tggctaaagc atcagaaaag ttctacggaa caggaagaag
     2581 aaaaaaatca atcgcaagag tgtatttagt acccggcaag ggtgacatta caataaataa
     2641 aagaagcatt gacgactatt tcggccttga gacattaaag acgatcgttc gtcagcctct
     2701 ttcagcaaca gagacagtag ataagttcga cgtacttgtc aatgttcacg gcggcggtta
     2761 tacaggacag gcaggcgcga tcagacatgg aatcgcaaga gcgcttttaa aggcagacgt
     2821 agaataccgt ccgaccttaa agtcagcagg attcctgaca agagaccctc gtatgaaaga
     2881 aagaaagaaa tacggt
//
LOCUS       DAOSYE010000229         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25739_length_2891_cov_3.151269, whole genome shotgun sequence.
ACCESSION   DAOSYE010000229 DAOSYE010000000
VERSION     DAOSYE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25739_length_2891_cov_3.151269"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLU43_04965"
     CDS             <1..406
                     /locus_tag="PLU43_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOO27792.1"
                     /translation="CFSYEHAFLEKEAGVLYPKLAETLAALAKRYPLFIVSNCQAGYI
                     ELFLKKTGFGEYFRDFTCPGDTGEMKAENIMLIAKRNKLNAPVYVGDTQLDADACAQA
                     GVRMVYASYGFGIVKCPDYVINTPSELLGLQF"
     gene            514..2118
                     /locus_tag="PLU43_04970"
     CDS             514..2118
                     /locus_tag="PLU43_04970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO27793.1"
                     /translation="MELTFVGAAHEVTGSCHFLKASGKNILVDYGMEQGENIYENVKI
                     PVEESAVDFVFLTHAHIDHSGLLPLLYARGFRGKIFATEATVDLCSIMLRDSAHIQMA
                     EAEWRNRKAKRSASVEEYEPIYNMRDADGAIKCLVPCHYGKEIEVCDGIRIRFTDVGH
                     LLGSSAIEVWITEPEGMKKIVFSGDIGNLQQPLIKDPITIEEADYVLIESTYGDRLHE
                     KDRLDYVTELTGILKETFDKGGNVVIPSFAVGRTQEMLYFLRQIKEDRLVKGHEDFPV
                     YVDSPLAVEATGVFNKNIYECFDEEAMELVNKGINPISFPGLNLSITSDESKAINFDN
                     TPKVILSASGMCEAGRIRHHLKHNLWRPECTVLFVGYQAVGTLGRILAEGVKKEVKLF
                     GETIEVRARILQLKGLSGHADKAGLTQWMLGFKKLPERVFIVHGDDEVCDHFADYLRE
                     EYGFDTYAPYSGTRVDLLTNTLTYEAEGIRISAKKKRQVSAVFDRLVAAGQRLLTIIQ
                     KNEGGTNKDLARFADQINSLCDKWNR"
     gene            2151..>2891
                     /locus_tag="PLU43_04975"
     CDS             2151..>2891
                     /locus_tag="PLU43_04975"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOO27794.1"
                     /translation="MSIADKVFIEMCKDILENGVSTEGEKVRPKWEDGSSAYTLKRFG
                     VVNRYDLRKEFPIITLRKTALKSATDELLWIWQKKSNNVHELSSHIWDAWADENGSIG
                     KAYGYQMSVKHQYKEGMMDQVDRVLYDLKNNPFSRRILTNIYVHQDLHEMNLYPCAYS
                     MTFNVTQRKGEERLTLNAILNQRSQDVLTANNWNVVQYAVLIHMFAQVCDMNVGEFVH
                     VIADAHIYDRHIPLVKELISRPVYEAPVF"
BASE COUNT          849 a          471 c          794 g          777 t
ORIGIN      
        1 gtgtttttct tatgaacatg cgtttctgga aaaggaagcg ggagtcctgt atccaaagct
       61 ggcggaaaca ctggctgctc tggcaaagag atatccgctg tttattgtaa gcaattgcca
      121 ggctgggtat attgaactgt ttttaaaaaa gaccgggttt ggagaatatt ttcgtgattt
      181 tacctgtcct ggagataccg gtgaaatgaa agctgaaaat attatgctta ttgcaaaaag
      241 aaataaattg aatgcaccgg tatatgtggg agatacgcag ttggatgcag atgcgtgtgc
      301 gcaggcggga gtaaggatgg tatatgcttc ttatgggttt ggaatagtaa agtgtccgga
      361 ttatgtgatc aatacgccgt cagaactttt gggactccaa ttttaaaaat cggagaatac
      421 tgttcttttc tgtaaaatga aaaagtggta tactgaaata cagaggtttt tatttttaaa
      481 atataaaaaa ttggacacac agggggtaag cgaatggaat tgacatttgt cggcgcagca
      541 catgaggtta ctgggagctg ccatttttta aaagcgtctg gaaaaaatat attggtcgat
      601 tacggtatgg aacagggaga aaatatttat gaaaatgtga aaataccggt cgaggaatca
      661 gcggtcgatt tcgtttttct gacgcatgca catattgatc attcaggatt actcccttta
      721 ttatatgcaa ggggatttcg cgggaagata tttgcaacgg aagcaacggt ggatctgtgc
      781 agtattatgc ttcgtgacag tgctcatatc cagatggccg aagcggaatg gagaaacaga
      841 aaagcaaagc ggtcggcgag tgttgaagaa tatgagccga tttataatat gcgggatgcg
      901 gacggtgcga taaaatgcct ggtgccttgt cattatggaa aagagatcga agtatgcgac
      961 ggaatcagga tccgctttac ggatgtcgga catctgcttg gttcgtcagc tattgaagta
     1021 tggatcacag agccggaggg catgaagaag atcgtatttt ccggtgatat cgggaatttg
     1081 cagcagccat tgatcaagga tcccattaca atagaggaag ctgattatgt tttgatcgag
     1141 tctacttacg gagacagact gcatgaaaag gaccgcttgg attatgtgac ggaactgacc
     1201 ggaattttga aggaaacatt tgataaaggc ggaaatgttg tgattccttc ttttgcagtc
     1261 ggacgtacac aggagatgct ctattttctg cgtcagatca aggaagacag acttgtaaag
     1321 ggacatgagg attttccggt ctatgtggat agtccgctgg cagttgaggc gaccggggtc
     1381 tttaataaaa atatttatga atgttttgat gaagaggcta tggaacttgt gaataaagga
     1441 atcaatccga tctcttttcc gggtctgaat ctttcgatta caagcgacga atccaaggcg
     1501 atcaattttg acaatacacc gaaggtgatc ttatcggctt ccggcatgtg tgaggccggc
     1561 aggatccgtc accatctgaa gcataattta tggagaccgg agtgtacggt tctgtttgtc
     1621 ggatatcagg cagtcggtac gcttggacgg attctggcag aaggggtgaa aaaggaagta
     1681 aagctgtttg gtgagacgat tgaggtacgg gccaggatcc tgcagctgaa aggcttgagc
     1741 ggtcatgccg acaaggccgg attaacacag tggatgctgg gatttaagaa actgccggaa
     1801 cgggtattta tcgtacatgg agatgatgaa gtctgtgatc attttgcaga ttatctgcgt
     1861 gaagaatacg gatttgatac gtatgcgcct tattcgggaa caagagtcga tctgttaaca
     1921 aataccctga cgtatgaagc ggaaggtatt cgtatcagcg cgaaaaagaa gcggcaggtt
     1981 tctgccgtat ttgaccgtct ggttgctgcc gggcagcgtc tgctcacgat catacaaaaa
     2041 aatgaaggcg gaacaaataa ggatctggca aggtttgcgg atcagatcaa ttctctttgc
     2101 gataaatgga acagataggg gaaatgggaa aataaatttg ggaggaagtt atgagtattg
     2161 cggataaggt ttttattgag atgtgcaaag atatattgga aaatggcgtc agtacggagg
     2221 gagaaaaggt cagaccaaaa tgggaggatg gttcaagcgc gtatacgtta aaacggtttg
     2281 gtgtcgtaaa ccgctatgat ctgcgaaaag agtttccgat cattacactg cgaaagacgg
     2341 cgctgaaaag cgcgacagat gagctgcttt ggatatggca gaagaagtcc aataatgtac
     2401 atgagctaag cagtcatatc tgggatgcct gggcggatga aaacggttcg atcggcaagg
     2461 catatggtta tcagatgagt gtgaagcacc aatataaaga agggatgatg gatcaggttg
     2521 atcgtgtact gtatgacctg aaaaacaatc cgttcagccg cagaattttg acaaatattt
     2581 atgtgcatca ggatctgcat gaaatgaatc tttatccgtg tgcttatagt atgacgttta
     2641 atgtgacaca gaggaaaggg gaagaaagac tgacactgaa tgcaattctg aatcagcgtt
     2701 cccaggatgt tctgaccgcg aacaattgga atgtcgtgca gtatgccgta ctgatccata
     2761 tgtttgcaca ggtctgtgat atgaatgtcg gtgaatttgt tcatgtgatt gcggacgcac
     2821 atatttacga ccgtcatatt cctcttgtga aagagcttat ttcaaggcct gtatatgaag
     2881 caccggtctt t
//
LOCUS       DAOSYE010000230         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25855_length_2883_cov_3.674328, whole genome shotgun sequence.
ACCESSION   DAOSYE010000230 DAOSYE010000000
VERSION     DAOSYE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25855_length_2883_cov_3.674328"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLU43_04980"
     CDS             <1..385
                     /locus_tag="PLU43_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO27795.1"
                     /translation="IYALLPMMIFAVYSSVEKMDWSLVEAARDIGAGPVKAFFTITLP
                     LTMPGLFSGIILTFIPSMGLFFIADILGGNKIVLVGSVIQEQLTKGRNLPFAAALAVV
                     LMVLTFVLIRIYRKLFHTKQLEGLV"
     gene            382..1170
                     /locus_tag="PLU43_04985"
     CDS             382..1170
                     /locus_tag="PLU43_04985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27796.1"
                     /translation="MRKKRKSSFPHIYLALLIIFMYIPIVVTVFYSFNESKISSIWGG
                     FSLKWYRQLFADRDLLQALFNSVLLAAISCVLAIIIGTFGAYGMYKTTSRWNDIISNL
                     STLPIMIPEIILGMVFLALFSFMGIPFGMITLVIAHTSFCIPYIFIMVSARLIGMEKS
                     FEEAARDLGASQACAFFDIILPLIVPAILSGTVLAFAMSFDDVVISIFTTGPKVSLLP
                     VKIYTKLKTGVTPEINALATVILIVTLGLIILTEWLNGRKKKNG"
     gene            1210..2268
                     /locus_tag="PLU43_04990"
     CDS             1210..2268
                     /locus_tag="PLU43_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO27797.1"
                     /translation="MKKFLVILMTAAIAGSMLVGCGSASKGESGLSGELNLYTWDGMF
                     PQELLDNFEAETGVTINYSNFDYDEDMLAKLEETKGGDYDLVIADDYIIELLIEEGLA
                     QKLDKSQIENFANINPVYQGYFYDPTDEYTVPVGAGVPLIVYNKDATGFEITSYEDLW
                     DPRLEDNVAITANYRVIDGITLKTMGESFNTEDLDTIKKAGKKLIDLAPNIRVINDNN
                     TQDYIVSGEVAAAFLYTSQVTAALQADPELSVAYPSEGLGFGIMAAFIPSEAPNAAAA
                     NAFLSYLLQPENGAEYAEYLGYYCTNKAAEEFISEDMKSLITLPDTVEKGEIIQNVSQ
                     EAEDQYSEIWKQFQEACN"
     gene            2636..>2883
                     /locus_tag="PLU43_04995"
     CDS             2636..>2883
                     /locus_tag="PLU43_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO27798.1"
                     /translation="MEKEKFYITTAIAYTSGKPHIGNSYEIVLADSIARFKRQQGYDV
                     FFQTGTDEHGQKIELKAQEEGVTPKEYVDKISGVVKGL"
BASE COUNT          824 a          513 c          756 g          790 t
ORIGIN      
        1 gatctatgcg ctgcttccca tgatgatctt tgccgtatat tcgagtgtgg aaaaaatgga
       61 ctggtcgctt gtggaagcgg caagggatat cggcgccggt ccggtaaaag cgttttttac
      121 gatcacgctg ccgcttacga tgccgggtct gttttccggt atcattctga catttattcc
      181 gtctatgggc ctgtttttta ttgcagacat attaggaggc aataaaatcg tacttgtcgg
      241 cagtgtaata caggaacagc tgaccaaggg aagaaatctg ccgtttgcgg ctgcgctggc
      301 tgttgtgctt atggtgctga cgtttgtgct tatccgtatt tacagaaaac ttttccatac
      361 gaaacagctg gagggacttg tatgagaaaa aaaagaaaaa gcagcttccc gcacatttat
      421 cttgcattat tgataatatt catgtatatt ccaatagtag taacggtatt ttattcattt
      481 aacgaatcaa agatctcttc gatatgggga ggcttcagcc tgaagtggta tcgccagtta
      541 tttgcagaca gagatttgct gcaggcgctg tttaacagtg tgctgcttgc tgcaatcagc
      601 tgtgttcttg caattattat tggaacattt ggtgcatatg gtatgtataa aacaacgtcc
      661 agatggaatg atatcatttc aaacctttcc acacttccga ttatgattcc ggaaattatt
      721 ttgggaatgg tatttttggc cttattttcc tttatgggga ttccgtttgg catgatcacg
      781 cttgtgatcg cgcatacgtc attttgtatt ccctatattt ttattatggt cagtgcccgg
      841 ctgatcggta tggaaaaaag ctttgaagag gcagcgcggg atttgggtgc gtcgcaggcg
      901 tgcgcatttt tcgatattat actgccgctg atcgtgccgg ctatcttatc cggaaccgtg
      961 ctggcgtttg cgatgtcttt tgatgatgtt gtaatcagta tttttacaac aggaccaaag
     1021 gtcagcctgc tgccggtcaa gatctatacg aaattgaaga caggcgttac gccggagatc
     1081 aatgcgctgg caaccgtgat cctgatcgtg acgctcgggc tgatcatact gacggaatgg
     1141 ctgaacggca gaaaaaagaa aaatggataa gagcatgtgg aattttgaag aaccgaagga
     1201 ggagcagtta tgaaaaaatt tctggtaatt ttaatgacag cggcaatagc aggaagtatg
     1261 cttgttggct gcgggagtgc gtctaaaggc gaaagcgggt tatccggaga actgaatctg
     1321 tatacatggg atgggatgtt tccgcaggag cttttggata attttgaggc ggaaaccggt
     1381 gtcacgatca attattctaa ttttgattat gacgaggata tgcttgcaaa gctcgaagaa
     1441 acaaaaggcg gcgattatga tcttgtaatt gcggatgatt atattattga actgttgatt
     1501 gaagagggac tggcgcagaa gctggacaag agtcagattg aaaattttgc aaacattaat
     1561 ccggtctatc agggatattt ttatgatccg acagatgagt atacggtacc ggtaggtgcc
     1621 ggagtgcccc tgatcgtcta taacaaggat gcgaccggat ttgagatcac aagctatgag
     1681 gatctgtggg atccgagact ggaagacaat gttgccatta ccgcaaatta cagagtcatt
     1741 gatggtatta cattaaagac aatgggagaa tcgtttaaca cggaggatct ggatacgatc
     1801 aaaaaggcag ggaaaaagct gatagatctt gcgccgaata tccgggtaat caatgataat
     1861 aatacacagg attatatcgt cagcggtgaa gttgcggcag cgtttcttta cacatcccag
     1921 gtgacggcag cattacaggc agatcctgaa ttgtcggtag cttatccgtc ggaaggtctg
     1981 ggatttggga tcatggcagc ctttattccg tcagaggcac cgaatgccgc agcggccaat
     2041 gcatttcttt cctatttgct gcagccggaa aatggagccg aatatgcgga atatttaggt
     2101 tattattgta caaataaagc tgcggaagag tttatttccg aagatatgaa atccctgatc
     2161 acacttccgg atactgttga gaagggtgaa atcatccaaa atgtcagtca ggaagcggaa
     2221 gatcagtaca gtgagatctg gaaacagttc caggaggcat gtaattaaat gattgaaaaa
     2281 cagtctcttg acagcaaaaa atattaatga tagtcttaag ataatctttt tggaaaaagc
     2341 agtgataaag agagtaagca gttaaggaat gctacagaga aatcctccgt ctggaaaaga
     2401 cgtgtgctga gagggataca ggagagactg ctgaatatgg cttttgagca gtgcaccgaa
     2461 tcggctgatt ttacgggtag agattatccg gaccggccgg caaggctgca acaggtccgc
     2521 ctgttatagc ggaaaatgag gtttgcgtat tttgcagacg aacagaagtg gtatcacgga
     2581 atcatgcccg tcttctttta tgagaagacg ggttttattt tggaaggaga acagaatgga
     2641 aaaggagaaa ttttatataa caacagcaat tgcctacacg tccgggaagc cgcatatcgg
     2701 caacagctat gagatcgtac tggcagacag tattgcgaga tttaaaaggc agcagggcta
     2761 tgacgttttt tttcagacgg gaacggacga acatggacaa aaaattgaat taaaggcaca
     2821 ggaggaaggc gttacgccaa aagaatatgt ggataagatc tcaggtgtcg taaagggcct
     2881 ttg
//
LOCUS       DAOSYE010000231         2875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_25984_length_2875_cov_4.136170, whole genome shotgun sequence.
ACCESSION   DAOSYE010000231 DAOSYE010000000
VERSION     DAOSYE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25984_length_2875_cov_4.136170"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLU43_05000"
     CDS             <1..242
                     /locus_tag="PLU43_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27799.1"
                     /translation="GNPTRKMIDPIISEQDAEEILVLFAKINSRNVLPTEDELNDPER
                     EAFDRKVLQSIGHEDLYNSIRKSLLSMQYTRHSIG"
     gene            267..>2875
                     /locus_tag="PLU43_05005"
     CDS             267..>2875
                     /locus_tag="PLU43_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOO27800.1"
                     /translation="MNTEKYMILIRGEIKTPEIRYCKYNSAAKKWDVAFNNGKTYSYR
                     YQNVEWLKDPVVLNPNSCRISRDGQDFFDVQAIYVFKSVHGAYWHICFGNGSKRDYAR
                     SELKVTESCLNRKQAGDVLKYIKQTAGLIDLRGKDTGEKILAKRFEKIDFVGADVALA
                     KYLDPCSLQTKTSAGSEYIPIFPFGCNNSQYKAVQNAMENQISVIQGPPGTGKTQTIL
                     NIIANILMQGKTVQIVSNNNSATENIYEKLSSSKYNLGFMVAVLGNAENKKAFVNKQN
                     AAYPDFSLWRLKADENVLLKSIREESLQLRDIFNKQEELAVLKQEQSQLAIELEYFNQ
                     YLEETDVDPDAIKVRKRLSAKSWMLLWQECRVVSEEKQKIGVLFKIKSFFRYGITNVS
                     FYKQDTAKVLAAFQTMYYRARKIELAGRILEIERSLKAAGSRLSDTMCGRSMAILKAR
                     LAGRYAGKTSRILFNEDDLWKNPYEVMKEYPVILSTTFSSRNSLHTKVIYDYLIMDEA
                     SQIDVATGALALSCAKNAVIVGDTKQLPNVVTDDIRKRAEAVFDAFHVNKGYQFTNSF
                     LKSVLEVMPNVTQTLLREHYRCHPKIINFCNQKFYRGELIIMTEDKGEEDVLAVVKTA
                     KGNHARNHYSQRQIDIIRDEIIPGYVTDADKTGIIAPYKNQVKALEKEFGNIDAATVH
                     KFQGREKETIIISTVDDEITDFADDPYLINVAVSRAKKKLMLVVTGNEQSQERNITDL
                     IVYIQYHNFACTESKIYSIFDYLYKQYTKERRLYLQKHRRISEYDSENLMYALLEDVL
                     KADQFACLDILCHFPMNMLIRNLDLLDEQECRYAMNPATHLDFLLYNRISKKPVLAIE
                     VDGYEYHKEHTTQA"
BASE COUNT          996 a          445 c          686 g          748 t
ORIGIN      
        1 gtggcaatcc cacgagaaaa atgattgacc caataatttc agagcaggat gcggaagaaa
       61 ttcttgtact gtttgccaaa ataaattctc gtaatgtctt gcctacagag gacgaactga
      121 atgatccgga gagagaggca tttgatcgta aagtgctgca atcaattgga catgaagatt
      181 tatataattc tatcaggaaa tcactattgt caatgcagta tacacgacac agcatagggt
      241 agacaatccg aaggatgaaa cgtgcaatga atactgaaaa atatatgatt cttataaggg
      301 gcgaaatcaa aacgccggaa atcagatatt gcaaatataa tagtgccgcg aaaaaatggg
      361 atgttgcatt taataacggg aagacatact cctacaggta tcaaaatgtt gaatggctaa
      421 aggacccggt cgtgttaaat ccaaatagct gccgtatcag cagagacgga caggactttt
      481 ttgatgtaca ggcaatatat gtttttaaga gcgtacatgg tgcctattgg catatttgtt
      541 ttggcaacgg cagcaagaga gattatgcac gcagtgagct taaagttacg gaatcctgtt
      601 tgaatcgaaa acaggcagga gatgttttaa aatatatcaa acagacagca gggttgattg
      661 atctgcgtgg taaagatacg ggggaaaaga ttcttgccaa aaggtttgaa aaaatagact
      721 ttgtcggagc agatgtcgca ttggcgaaat atttggatcc gtgttcattg cagacaaaaa
      781 caagtgctgg ttcagaatat atcccgatct ttccttttgg ctgtaataac agccagtata
      841 aggcagttca gaatgcgatg gaaaatcaga tcagcgtgat tcagggaccg ccgggaaccg
      901 gaaaaacgca gacgatcctg aatattattg caaatatatt aatgcaggga aaaacagtgc
      961 agatcgtttc caacaataat tcggcgacgg aaaatatata tgagaagctg tcatcttcaa
     1021 aatataattt aggctttatg gttgcagttt tggggaacgc agaaaataaa aaggcatttg
     1081 ttaataaaca gaatgccgca tatcctgatt tttcattatg gagactaaaa gcggatgaga
     1141 atgttttact aaaaagtata agggaagaat cactgcagct tagagacatc ttcaacaaac
     1201 aggaggaatt ggcggttctg aaacaggaac agtcgcaact ggcaattgaa ttagaatatt
     1261 ttaatcaata tttagaagaa acagatgttg atccggatgc cataaaggta agaaagcggc
     1321 tgtcggcaaa aagctggatg ttattgtggc aggagtgtcg ggtggtatct gaggaaaaac
     1381 agaaaatagg agtattattt aaaattaaaa gcttttttcg atatggaatt acaaatgtca
     1441 gcttctataa acaggatacg gcaaaggtcc tcgcagcatt tcaaacgatg tactatagag
     1501 cgagaaagat agaattagca ggcaggatat tggaaataga acgttcttta aaagcagccg
     1561 gcagccggct gtcagatact atgtgcggtc ggtcgatggc gatattaaag gccaggctgg
     1621 cggggagata tgctggaaaa acaagccgca tactatttaa tgaagatgat ttatggaaaa
     1681 atccatatga agttatgaaa gaatatccgg taattttaag cacgacattt tcatcaagga
     1741 atagtctgca tacaaaagta atatacgatt atctcattat ggatgaggca tcacagattg
     1801 atgttgcgac aggggcactg gcattgtcct gcgcgaaaaa cgctgtaatt gtaggtgata
     1861 caaaacagct gccgaatgtt gttacggatg atatcagaaa acgggcagag gcagtatttg
     1921 atgcgtttca tgttaataaa gggtatcagt ttacgaatag ttttctgaaa tcggtattgg
     1981 aggttatgcc aaatgtaaca cagacattgc tgcgggaaca ttacaggtgc catcctaaaa
     2041 ttatcaattt ctgcaatcaa aagttttaca gaggggaatt gatcattatg actgaggata
     2101 agggagagga ggatgtgctg gcagtagtaa aaaccgcgaa gggcaatcat gcgcgtaatc
     2161 actacagcca gaggcagatc gacataataa gggacgagat tattccaggg tatgttacgg
     2221 atgcggacaa aacaggaatc atagcacctt ataaaaatca ggtcaaggca ttggaaaagg
     2281 aatttggcaa tattgacgct gcaacagtgc ataagtttca gggaagagaa aaggaaacaa
     2341 ttattatatc aacggtagat gatgaaataa cggattttgc agatgatcca tatctgatca
     2401 atgttgccgt atcaagagca aagaagaagc tgatgctggt tgttacaggt aatgaacagt
     2461 cacaggagcg caacattacc gatctgattg tatatatcca atatcataat tttgcatgta
     2521 cggaaagtaa aatatattcg atatttgatt atttgtataa acaatataca aaagaacgaa
     2581 gactttattt gcagaagcac aggagaattt cagaatatga ttcggaaaac ctgatgtatg
     2641 cattattgga agacgtgctt aaggcagatc agtttgcctg tctggatatt ttgtgtcatt
     2701 ttccgatgaa tatgttaatt aggaatttgg atttattaga tgagcaggag tgccgctatg
     2761 caatgaatcc ggccacacat ctggattttc tgttatataa caggatcagc aagaagccgg
     2821 tattagccat tgaagttgac gggtatgagt atcataagga gcatacaaca caggc
//
LOCUS       DAOSYE010000232         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26096_length_2867_cov_4.761735, whole genome shotgun sequence.
ACCESSION   DAOSYE010000232 DAOSYE010000000
VERSION     DAOSYE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26096_length_2867_cov_4.761735"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLU43_05010"
     CDS             complement(<1..268)
                     /locus_tag="PLU43_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO27801.1"
                     /translation="MLIDRKRALQEINISEKMFNELLNVFIEQTEPALKILEEKAEKR
                     KYEEIRQKAHFIGGSAGNLRIEGIRSLAKEIELEAMEKREISVIK"
     gene            773..2701
                     /locus_tag="PLU43_05015"
     CDS             773..2701
                     /locus_tag="PLU43_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27802.1"
                     /translation="MDRSFNLRKSVVVFSILAALWLLKHPYYGIWHDGVLYTFLALKD
                     LYPHAYTSDLFIKFGSQGNFTLFPQIYAGFINIFGIYKSALLLTIAGQFLWIFAAVTL
                     LRTFLQGKQLYIALAIVFFMSAFYGGLHCFRYAEMFVCPRIFSEALCIFATACIIKKR
                     YLASGALILVSFFIHPLISLGAGLIFYIYFVLTYPRFLFSAPFLLAVVLLLGFFKIEP
                     FTGLFSIIQPEWHQIIKIRSSFMLLTEWPLLSWTELFFTISVTAAACLFSNAFLRRLF
                     LASIIGACCGMLINMIGGELLKIELIMKIQIWRALWLLQFISAIGAAVIIFNFLKEKN
                     NKLLLCAFFLLTSYSISLGVISFAITLLFFYLCVRVSRNLLPSVHIPQPAVFLVTGII
                     ITMIILSSAAKEISVLIDHNFNTQYLASVLEAIPLDLFTPLILFFVVFFFYFHLHQTI
                     SFKIIFLSLAVLIFSLSVWDRQSPWSKMLERNKDTTAFFRNLLPPEATILWDHSPEIS
                     WFGARRSSYISTKQGSGIVFYQQTALEYAKRINHILPLMDENPLTSKGFMYKPGWEIN
                     QNKFKSNYKQVCRNAKELDYIVSTENIPGESLAHWHSKIPLVKTRFLENGTFEREAHQ
                     DFYLYDCSMPREGLQKWN"
BASE COUNT          752 a          617 c          516 g          982 t
ORIGIN      
        1 ttttgataac agaaatttcc cgtttctcca ttgcctccaa ttctatttct ttggctaacg
       61 aacggatgcc ttcgatccgc agattgcctg ccgaaccgcc gatgaaatgc gctttttggc
      121 ggatttcttc atattttctt ttttcagctt tttcttccag tattttcaaa gcaggttcgg
      181 tctgctcgat aaaaacattc agcaattcat taaacatttt ttcggaaata ttgatttcct
      241 gcagagctct ttttctgtcg atcaacatga gatttctccg gagaccatct ttcatccctg
      301 tcatcctttc ggtttgacaa aatgcgttct gatgatccgc caccgctgaa attatagctt
      361 aaattataaa ttttctttat atattacaac aaaatgaacg gattgtatag tccttcttta
      421 gtgttttatt tctcattgac aaatgaaaga atttattatt tatagatcga caagttgtta
      481 taaaaaattc ttaaaggggg tattttttat gattaatgca ggttgcggtg gttcatctga
      541 aacctaaaaa attgaaaaaa taggcaggga gtaacgaaaa agagggtttt tctgttttcc
      601 ctgcctattt ttatgatgtg tacggcagca tgattcttat ctaccgtaat catacacata
      661 ccccttgatt ggcttcacag cacaaactca atcttcagct tcctatccta ctgaagatgt
      721 tatattttca aaaatatttt ttttcacaaa tcattatcgg aaaaaatatg cgatggatcg
      781 atctttcaac ctgagaaaaa gtgttgtcgt tttttccata cttgccgccc tttggcttct
      841 caagcatccc tattacggaa tctggcatga tggggtttta tacacttttt tggctctgaa
      901 ggatttatat ccccatgcat ataccagcga ccttttcatt aaattcggct cccaaggcaa
      961 ctttacttta ttcccccaaa tctatgccgg cttcattaac atatttggca tatataaaag
     1021 cgctctgttg ctgacgattg ccggtcagtt cctctggatt tttgctgcgg tgacattgct
     1081 ccgtacgttt ctgcagggga agcaactgta tatcgctttg gcgatcgtct tttttatgag
     1141 cgctttttat ggcggattgc attgctttcg atatgcagaa atgttcgtgt gcccaagaat
     1201 attttcagag gcactttgca ttttcgccac cgcctgcatt attaaaaagc gctatcttgc
     1261 ttcaggggcc ttgatccttg tctctttttt tatacatcca ctgatctctc taggggctgg
     1321 tcttattttc tatatctatt tcgtcctgac ttatccccgt tttctatttt cagccccttt
     1381 ccttttggca gtcgttttat tgctgggatt ttttaagata gaacctttca cgggattatt
     1441 cagcatcata caacctgaat ggcatcaaat cattaagata agatccagtt tcatgttatt
     1501 aaccgaatgg cctttgcttt catggactga gctttttttt acaatctcgg taacggctgc
     1561 ggcctgtttg ttttccaacg cgttcctgcg tcgtctcttt ctggcttcta tcatcggggc
     1621 ttgttgcggg atgttaatta acatgatcgg gggcgaactt ttaaagattg aattaatcat
     1681 gaagatacaa atctggagag ccctctggct tctccaattt atctcagcca tcggcgcagc
     1741 tgtcattatt tttaatttcc tgaaagagaa aaacaacaaa ttattgctct gtgcattttt
     1801 tctgctgaca agctatagca tttccctggg cgtcatcagc ttcgccatca cccttctatt
     1861 tttctatctt tgtgtacggg tgtccagaaa ccttctgccg tctgttcata taccgcaacc
     1921 cgctgtattt ctggttacag gcatcatcat tacaatgatc attctctctt cggcggcaaa
     1981 agagatcagc gtattgatag atcataattt taatacgcaa tacctggcaa gcgttcttga
     2041 agccattcct ctggatcttt ttactccctt gatattattt tttgtcgtct tttttttcta
     2101 ttttcatttg catcagacaa tttcttttaa aattattttt ctatcgttag ccgttttgat
     2161 cttctcgctt tcagtatggg accgtcagtc tccatggtct aaaatgcttg agcgcaataa
     2221 agatacaaca gcctttttca gaaacctgct acccccggaa gctaccattc tctgggatca
     2281 ttctcccgag atttcctggt tcggagcaag aaggtcttct tacatctcca cgaaacaagg
     2341 ttcaggcatt gtgttctatc agcaaacagc gcttgaatat gcaaagcgga tcaatcacat
     2401 tcttccgttg atggatgaaa atcccctgac gtcaaaaggt ttcatgtata aaccgggatg
     2461 ggaaatcaat caaaataaat ttaaatcaaa ttataagcag gtatgccgga acgccaagga
     2521 gctggattat atcgtctcga cagaaaacat cccaggagaa tcccttgccc actggcattc
     2581 aaaaatcccc ctggtaaaaa cacgtttttt agaaaacggc acgttcgaaa gggaggctca
     2641 tcaagatttt tatctttatg attgcagcat gcccagggag ggattgcaaa aatggaatta
     2701 aataaaacca cattagaata tctggaagat cattatggga aaagcatcct tccgttgtcc
     2761 aaattcgaaa atgaatactt tatgggtaca agccccatag aagttttttt cctgtataag
     2821 aaaaacaatt ccatcttcac gatgaaagag agccttttaa aaaccat
//
LOCUS       DAOSYE010000233         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26101_length_2867_cov_2.651138, whole genome shotgun sequence.
ACCESSION   DAOSYE010000233 DAOSYE010000000
VERSION     DAOSYE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26101_length_2867_cov_2.651138"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLU43_05020"
     CDS             <1..747
                     /locus_tag="PLU43_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO27803.1"
                     /translation="PVYLLGGPMMGRIGRAQDVVTKTTNAVLVLPQEHQIVQRMRSSS
                     SIDMKRAASACCQCQMCTDLCPRHLLGHPIEPHRFMRSAANRDFQDLQPFLDTMFCSS
                     CGLCELFSCPQGLSPRTLIAEYKAGLRKAGVKLPDVTAAPVLEARAYRKAPEARLEAR
                     IGLYKYHKPAPISHTQAEASKVKLLLSQHIGAPAVPVVAKGETVTAGQMIAGPADGLS
                     TAIHASIDGMVTAVEKNYIMIENRKGRADR"
     gene            744..1292
                     /locus_tag="PLU43_05025"
     CDS             744..1292
                     /locus_tag="PLU43_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOO27804.1"
                     /translation="MSKAIGMIEFKTVSTGVTAADAMVKTAEVDIVEAETVCPGKYIA
                     IISGDLSAVKAAVETATTVYAEQLINSFVLGNPHESIFPAIYGSTEVENPRALGILET
                     YDAASIIVAADVAAKTSLVELIELRIAKGMCGKSYMFLTGEVAAVEAAIEKAISSVAK
                     EGTYLDSSVIAHPDEKLMKSII"
     gene            1360..2127
                     /locus_tag="PLU43_05030"
     CDS             1360..2127
                     /locus_tag="PLU43_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOO27805.1"
                     /translation="MLALERRNLILEKLQEEKRVVVGELSQLYGVSEETIRRDLDKLD
                     KDGLAIKSYGGAVINENTSIDMPFNIRKKRNVSGKQKIAEFVAGLINDGDHIILDAST
                     TAVFITKAIKQKSRLTVITNSIEIMIELSDVSDWNIISSGGSLKEGYLALVGPQAING
                     LASYNVEKAIISCKGIDMEKGITDGNEQFSQAKQVMQKVSKQNILAVDSSKFGEVAFS
                     KICDVSEMDVVVTDEKPDERWLGFFEKCGIQCLYPEA"
     gene            complement(2138..>2867)
                     /locus_tag="PLU43_05035"
     CDS             complement(2138..>2867)
                     /locus_tag="PLU43_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27806.1"
                     /translation="INGRTKITLAEGELLLLGQHTTHEIFPAGENDIGVNFIVLPEFF
                     DQIVAMMDEENVLRDFLILTLRQKSGAADYFHFQSADILPVQNLAENLIWSLMNSQPN
                     RRNINQTTMGLLFLHLMNNTEQINKNDPARYEQNLTFTVLKYIENHYRTASLTEIAKL
                     LKIPPYCISKTVKKQAGYTFKQLLQTKRLNQSAFLLSSTSLPVEDIIALAGYDNTSYF
                     HKIFKERYNMTPRAYRLAAAETRS"
BASE COUNT          778 a          578 c          794 g          717 t
ORIGIN      
        1 cctgtctatc tgctcggcgg accaatgatg gggcgtatcg ggagggcaca ggatgttgta
       61 acgaaaacga caaatgctgt tttagtattg ccgcaggagc accagatcgt ccagagaatg
      121 agaagctctt cgtcgatcga tatgaaacgt gcggcgtctg cctgctgtca gtgccagatg
      181 tgtacggatc tgtgtccgag gcatcttctt ggacatccga tagagccgca tcggttcatg
      241 cgttctgcag cgaaccgtga ttttcaggat ctgcagccat ttctggatac gatgttttgt
      301 tcttcatgcg gattgtgcga attgttttcc tgtcctcagg gcttgtcccc cagaacgctg
      361 atcgcggaat acaaagccgg actgcgaaag gcaggggtaa agctgccgga tgttactgcg
      421 gcgcccgtgc tggaagcgag agcctatcga aaagcaccgg aagcaaggct ggaggcgagg
      481 atcgggcttt acaagtacca taaaccggca ccgatcagtc atacgcaggc ggaggcgtcc
      541 aaagtgaaac tgctgttaag tcagcatatc ggcgcgccgg ccgtaccggt tgttgcaaag
      601 ggagaaacgg taacggcagg gcagatgatc gcagggcctg cggacgggtt aagtaccgct
      661 atccacgcgt caatagacgg tatggtaact gcagtggaaa aaaattatat tatgatagaa
      721 aacaggaagg gacgtgcgga tagatgagca aggcaattgg aatgatagaa ttcaaaacgg
      781 tatcgaccgg ggtaacggca gcggatgcaa tggtcaagac cgctgaagtg gatatcgtcg
      841 aagcagagac cgtatgtccg ggtaaatata tcgcgatcat atcaggagac ttgagcgcgg
      901 tgaaggcagc tgttgaaacg gcaacgactg tttacgcgga acagctgatc aacagctttg
      961 tactcggaaa tccgcatgaa tcgatttttc cggcaatata tggatccacc gaggtggaga
     1021 atccgagagc gctcggcatt ctggagacgt atgatgcggc atccattatc gtggcggcgg
     1081 atgtggctgc aaaaacttcc cttgtcgaac tcatagagct tcgtattgca aaaggaatgt
     1141 gcgggaaatc atatatgttc ctgaccggag aagttgcggc agtcgaggca gcgatcgaaa
     1201 aggcgatctc atctgttgcg aaggaaggaa catatctgga ctcttccgtg atcgcgcatc
     1261 cggatgaaaa gctgatgaaa tccattatat agtatttgtg ttttcaaaaa aaacagtgta
     1321 aactttaagg catgaaatat tcacaacggg aggtatggca tgcttgcatt ggagagaagg
     1381 aatttaattt tagaaaagct gcaggaggaa aagcgggtag tagtcggcga attgagtcag
     1441 ctttacggcg tgtctgagga gacgatccgc agagaccttg ataagcttga caaggacgga
     1501 cttgcaatca agagctacgg cggtgccgtg atcaatgaga atacaagtat tgatatgcct
     1561 tttaatatcc gtaaaaaaag aaatgtatcg gggaaacaga agattgccga attcgttgca
     1621 gggctgatca acgacggaga tcatattatt ctggatgcca gtacgactgc ggtgtttatt
     1681 acgaaagcga tcaaacagaa gagccgtctg acagtcatta cgaattccat agagatcatg
     1741 atagaacttt ccgatgtgtc cgactggaac attatttctt cagggggaag tctgaaagaa
     1801 ggatatctgg cacttgtcgg cccgcaggcc attaacggac ttgcgtctta taatgtcgaa
     1861 aaggcgatca tttcctgcaa ggggatcgat atggaaaaag ggatcaccga tggaaatgag
     1921 cagttttcac aggcaaagca ggtcatgcag aaggtgtcga agcagaatat cctggcggtg
     1981 gacagctcta aattcggaga agtcgctttt tcaaagatct gcgatgtatc tgaaatggat
     2041 gtggtcgtta ccgatgaaaa accggatgag cgctggcttg gtttttttga aaagtgcggt
     2101 attcaatgtc tgtatccgga ggcgtagtcg gtttgtgtca ggaacgtgtt tccgctgctg
     2161 ccagccggta ggcgcggggc gtcatgttgt atcgttcttt gaagatctta tgaaaatagc
     2221 ttgtgttgtc atatcccgca agtgcaataa tatcttctac cggaagagag gtagaggaaa
     2281 gaagaaaggc tgactggttc agcctttttg tttgcagcag ctgcttaaac gtatacccgg
     2341 cctgtttttt caccgttttg ctgatgcagt agggcggtat tttcagcagt tttgctattt
     2401 ctgtcagcga tgcggtgcga taatggtttt caatatattt taaaacagta aacgtcagat
     2461 tctgctcata ccgggcagga tcatttttat tgatttgctc cgtattgttc attaaatgca
     2521 aaaataacag tcccattgtc gtctggttga tattccggcg gttcggctga ctgttcatca
     2581 gagaccagat caggttttcg gcaagattct gtacgggaag gatatcggct gactgaaaat
     2641 gaaaataatc tgccgcaccg gatttttgtc tcagtgtcag gatcagaaaa tcacgcagaa
     2701 cattttcttc atccatcatg gcaacgatct gatcaaaaaa ttcagggagc acgataaaat
     2761 taacgccaat atcgttttcc ccggcaggaa aaatctcatg ggtcgtatgc tggccgagca
     2821 gaagcagttc accttccgca agcgtgatct ttgttctgcc gttgatg
//
LOCUS       DAOSYE010000234         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26279_length_2856_cov_2.826848, whole genome shotgun sequence.
ACCESSION   DAOSYE010000234 DAOSYE010000000
VERSION     DAOSYE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26279_length_2856_cov_2.826848"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLU43_05040"
     CDS             <1..678
                     /locus_tag="PLU43_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO27807.1"
                     /translation="GEDWTGGFEGPANVELESVKNMLNMQVDWLNSGVAEVSPDGHVD
                     LEAGFQNILDHNIVSFPKAMWYMSRFINYMPEESGNWYIAPCPVFEAGQNNSVGIGGT
                     GTVVTQQSSDPALAADFLCYAKMSMEGEQMNWEMLGFDVCNTEMWTDESIIHDDSNQY
                     NQFFRNYPYDVLNEIKDGIGKISVVAISPTINEDMCNVTLNAILEDGTPVDEALATSQ
                     ATIDLEQ"
     gene            764..1660
                     /locus_tag="PLU43_05045"
     CDS             764..1660
                     /locus_tag="PLU43_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27808.1"
                     /translation="MKLKKFLYSQKAAPYVFVLPFILSFLIFWIYPLCSTITMSFQEI
                     KPLGTTWVGFKNYQKLLIDTVFHTAILNSFEYMLLTLIFLIPFPMLFAVLIDSRIVKA
                     KGVWKAILYLPALTSVVISGTLFRLMFSEYPTGQMNVLMTQLGFATQKWLKFRPTAYF
                     ALLVMACWRWTGVNMLYFLSGLKSINTAFYESANIDGASGWQKFRYITIPLLKPTTIY
                     VLTISIYAGLAMFLESFMLYTQNASPNNIGLTIVGYLYRRGIEKNQLGYASAVGLVLL
                     VIALIINIAQLILNGTFKKEEK"
     gene            1662..2552
                     /locus_tag="PLU43_05050"
     CDS             1662..2552
                     /locus_tag="PLU43_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27809.1"
                     /translation="MSDMTASVKIKSNASASRNKKIKSFFATAVFVVLCSLVVFPLLA
                     GLLASFRPGTELIRRGLSINFDVTTMTLDNYIYLFGGNADSLKYFMWYKNSLVLTLVT
                     VLLTLFVCYFIAYGLSMYEFKLKNLLFFLVIATMMVPFEILMLPLYKEMITLHLIDTM
                     AGVFLPGVCAASTIFFFKQYMTALPKELLDAGRIDGATEYGICVKIMLPITKPAFAAM
                     GILCAMGSWNNMLWPLLVFRSAGKFTLPIGLNTLLTPYGNNYDVLIAGSMFGIIPIFV
                     IFLFCQKYLIEGMTAGAVKG"
     gene            2606..>2856
                     /locus_tag="PLU43_05055"
     CDS             2606..>2856
                     /locus_tag="PLU43_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOO27810.1"
                     /translation="MKTKMTVDKAFVKSQIDSRLYGSFIEHLGRAVYHGIYEPENPAS
                     DSDGFRRDVIALVKELEIPVIRYPGGNFVSNFFWEDSVGP"
BASE COUNT          797 a          546 c          693 g          820 t
ORIGIN      
        1 ggcgaagact ggacaggcgg atttgaaggt cctgccaatg tagagcttga atccgtaaag
       61 aacatgctga atatgcaggt tgactggctg aattcaggag ttgcggaagt ttctccggac
      121 ggacatgttg atcttgaagc aggtttccag aatattcttg atcataatat cgtatctttc
      181 ccgaaagcaa tgtggtatat gagcagattt atcaattaca tgccggaaga gtcaggcaac
      241 tggtatatcg caccatgtcc ggtatttgaa gcaggtcaga ataattccgt aggtatcggc
      301 ggaacaggca cggtcgtaac gcagcagtct tcagatccgg cactggcagc agacttcctt
      361 tgctatgcga agatgtcaat ggaaggtgag cagatgaatt gggaaatgct tggatttgac
      421 gtatgtaaca cagaaatgtg gacagacgaa tctattattc atgatgattc caaccagtat
      481 aaccagttct tcagaaatta tccgtatgat gtgctgaacg aaatcaaaga cggtatcggc
      541 aagatctccg ttgttgcaat cagcccgacg atcaatgagg atatgtgtaa tgtaacatta
      601 aacgcaattc ttgaagacgg aacccctgtg gatgaggcat tggcaacgtc acaggcgacg
      661 atcgatctgg aacagtaaac aggttcaata aatgcagagg gaggggcccg gaatatttcg
      721 ggcactccct ttatatcagg aaggattcag aaggagaaag gttatgaagc taaaaaaatt
      781 cctctattca cagaaagcgg cgccttatgt atttgtattg ccatttattt taagttttct
      841 gattttttgg atttacccat tatgcagtac gattacaatg agctttcagg aaatcaaacc
      901 acttggaacg acatgggtcg ggtttaaaaa ttatcagaaa cttctgatag atacggtatt
      961 tcatacggcg atcctgaaca gctttgaata catgctgctg acattgattt ttctgattcc
     1021 atttccgatg ttatttgcag tgcttatcga ttccagaatt gtaaaagcca agggcgtatg
     1081 gaaagcgatc ttatatttgc cggcattgac gtccgttgtt atttccggta cattgttccg
     1141 tctgatgttt tcagagtatc cgacaggtca gatgaacgta ttgatgacac agcttgggtt
     1201 tgcgacacaa aaatggctga aattccggcc gactgcatat tttgcactgc ttgtcatggc
     1261 atgctggaga tggacgggtg taaatatgct gtattttctg tcaggattaa aatcgatcaa
     1321 tacggcattt tatgaatcgg caaatattga cggagccagc ggctggcaga aattccgcta
     1381 tattacgatc ccgttattga aaccgacgac tatttatgta cttacgatca gcatttatgc
     1441 cggacttgcc atgtttcttg aatcctttat gctgtatacg cagaatgcgt cgccaaacaa
     1501 tatcggttta acgatcgttg gttatttgta cagacgaggg atcgaaaaaa atcagcttgg
     1561 atatgcgtcg gcagtcggcc tcgtattgct ggtgatcgcg cttattatta acattgcgca
     1621 gctgattctg aacggaacgt ttaaaaagga ggaaaagtga catgagtgat atgacagcct
     1681 ccgtaaaaat aaaatcaaat gcaagtgctt caagaaacaa aaaaataaaa tcgttttttg
     1741 caacggcagt atttgtggtc ctttgctccc ttgtggtatt tccgctgctt gccggtctgc
     1801 tggcatcttt cagacccggt acggagctga tcagaagggg cctaagcatc aattttgatg
     1861 ttacgacaat gacacttgac aactatattt atctgttcgg cggaaatgca gactccctca
     1921 agtacttcat gtggtataag aacagtcttg tactgacgct cgtaacagtg ctgctgacat
     1981 tgttcgtatg ctattttatt gcatatggtc tgtcgatgta tgaatttaaa ttaaaaaatc
     2041 tgctgttctt tttagtaatt gcaacaatga tggtgccgtt tgaaatttta atgctgccgc
     2101 tttataagga aatgatcaca ctgcatctga tcgatacaat ggccggtgtg tttttaccgg
     2161 gtgtgtgcgc ggcttcaacg atctttttct ttaagcagta tatgacggct ctgccgaagg
     2221 aattgctgga tgcggggcga atcgacggcg cgaccgaata cggaatctgt gtgaagatca
     2281 tgcttccgat tacaaaaccg gcatttgcag caatggggat cctctgtgca atgggttcct
     2341 ggaacaatat gctgtggccg ctgcttgtat tcagaagtgc cggaaagttt acacttccga
     2401 tcggtttaaa tacgcttttg acaccgtacg gaaacaatta cgacgtatta atagccggtt
     2461 ccatgttcgg cattattccg atttttgtta ttttcctttt ctgtcagaaa tatctgatcg
     2521 aaggaatgac cgcaggagcg gtcaagggtt aaacaaagcc gtttattacg gccaatgata
     2581 aagaagatgg cagggataat agaaaatgaa aacaaaaatg acagtagata aggcttttgt
     2641 aaaaagccag attgatagcc ggctctatgg ttcttttatc gaacatcttg ggagagcggt
     2701 ctatcatggg atctatgaac cggaaaatcc ggcgtcggac agtgacgggt ttcgccggga
     2761 cgtgatcgca cttgtaaaag aactggaaat accggtcatc cggtatccgg gcggaaattt
     2821 tgtctccaat tttttctggg aggacagtgt cggacc
//
LOCUS       DAOSYE010000235         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26364_length_2851_cov_3.190272, whole genome shotgun sequence.
ACCESSION   DAOSYE010000235 DAOSYE010000000
VERSION     DAOSYE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26364_length_2851_cov_3.190272"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLU43_05060"
     CDS             <1..913
                     /locus_tag="PLU43_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO27811.1"
                     /translation="FIPLAVGTLLYMFFYGIQAVGSAGFYKAVFIGTIMTATSVSITV
                     QTLKELGHLKSKVGTAVLAAAIIDDVISIVVLTFVIGLKNPDTHIGKVVLSTLLFFVF
                     AIIVGYVSYKAFKWFDRRYPHTRRIPIAGLAYCFALSFIAEQYFGIADITGAYVAGII
                     LCNIRDSDYIAEKMDINSYMLFGPIFFASIGLKTDITDFSSNVLYFSIAFVLVALLTK
                     IIGCSIAAKACRFNWRDSMKIGVGMMTRGEVALIVSQKGLSVGLLEPVYFTSVIFLII
                     ISSVVTPILLKLLFSKSSGTPVGQPAS"
     gene            complement(943..>2851)
                     /locus_tag="PLU43_05065"
     CDS             complement(943..>2851)
                     /locus_tag="PLU43_05065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27812.1"
                     /translation="EQNIKVTKDMIRVGDFSESVEKEVEELFDLNPHVQAFVCANDLM
                     ANSVYKVCKKRRLEIYQMTPKAREWLKKSPLRYRIGRDIDRGEGVAVTGYDDWVLAAT
                     LDPPLTTVLQNAYSSGYKALFNVLRILNEGICENIILPPQLIRRNSCGCQDMEKYKFS
                     EITASEKLNPEFYAIKTAETIKDAILISDVNDAIGDKVYDYLYDVIYANTIIYLGYVK
                     EILSAKRVVEQLRILMTGPYADYISPSALATMFSDYLSFVIHTVDDHMAEVLFSEILI
                     EGMKYLQAHIYKKAKDSLSEYERSACFMSLISRDMVNHVDSEKEMLYNAMLKLKTLNF
                     GNSYLYMLKSPIMHMRGEKWVCPDEIYLAAQVVDGEITAYALKERPVVTAENGFGAYI
                     DSGGEKKGYQVTLINLYSDKMQYGVLVSEIRPENAISLYYASIQISTALKYYDISRAQ
                     KSTQERLEQLVKEVEEKNSMLRFISEYDSLTGCLNRSGFIEKAMNLKNSSFGSEAIII
                     FGDLDHLKEINDKFGHAEGDYAIKKIAQIIREAIGEGELISRIGGDEFVVMMLCDKDR
                     VPEEIITKVGSITEEFNKNSSKPYYVESSIGFQKFTCNETTELQDVMNQADAALYAAK
                     RVRRESIEKPQ"
BASE COUNT          735 a          695 c          529 g          892 t
ORIGIN      
        1 atttattccc cttgccgttg gcactctctt atatatgttt ttttatggca tccaggcagt
       61 cggttcggcc ggtttttata aggcggtttt tatcggtacg atcatgacgg ctacttccgt
      121 cagcattacc gtccaaacct taaaagagct cggacatttg aaaagcaagg taggaacagc
      181 tgttttggca gctgccatta tcgacgacgt catcagtatt gtcgtactga cctttgtgat
      241 cggtctgaaa aatcctgata cacatatcgg taaagtcgta ctcagcacac ttcttttctt
      301 tgtatttgca atcattgtcg gctatgtttc atataaagcc tttaaatggt ttgacagacg
      361 ctatccgcat acaagacgta ttccgatcgc cggactggct tattgttttg ccctgtcgtt
      421 tattgccgaa cagtattttg gaatcgcaga tattaccggc gcctatgtgg ccggaattat
      481 tctttgcaat attcgggatt cggattatat tgctgaaaaa atggacatca attcttatat
      541 gctgttcggt ccgatctttt ttgcaagtat cggtctcaaa acagatatca ctgacttttc
      601 atcaaatgtg ctgtatttct caattgcctt tgtacttgtt gccctgctca caaagatcat
      661 cggctgcagc attgctgcaa aagcctgccg gtttaattgg cgggactcca tgaagatcgg
      721 cgttggcatg atgacacgcg gagaggtcgc cctgatcgtt tcgcaaaaag ggctgtcggt
      781 tggtcttttg gaacctgttt attttacatc cgttattttt ctgatcatta tttcatctgt
      841 ggtcacaccg atccttttaa aactgctgtt ttcaaaaagc agcggaacac cggtcggaca
      901 gcctgcatcc tgaaataacg ccaaaacgcg tcggatgatt tatcactgcg gtttttcaat
      961 tgattcccgt ctgacgcgtt ttgccgcata aagcgcagca tccgcctgat tcattacatc
     1021 ctgcagttct gttgtctcat tacatgtaaa tttctgaaaa ccgatagaac tttctacata
     1081 atagggtttg ctggaatttt tattaaactc ttccgtaatc gatccaacct ttgttatgat
     1141 ctcttccggc acacgatcct tgtcacagag catcatcacg acaaactcat cgccgccgat
     1201 cctgctgatc agttctcctt cgccgatcgc ttcccttatg atctgtgcga tctttttaat
     1261 cgcataatcg ccttctgcat gaccgaattt atcattgatt tccttaagat gatccagatc
     1321 gccaaatatt ataatggctt cactgccaaa agatgaattt ttcaggttca tcgccttttc
     1381 aataaatccg cttctgttca ggcagcctgt cagtgaatca tattcggaaa taaaccgaag
     1441 catgctgttt ttttcctcaa cttcttttac cagctgttcc aaacgctcct gcgtgctctt
     1501 ctgagcacgg cttatatcat aatatttcaa tgcggtactg atctgaatac ttgcataata
     1561 cagagaaatg gcattttcag gcctgatttc actgacaaga acaccatact gcattttatc
     1621 cgaatataaa ttgatcagcg tgacctgata tcctttcttt tccccgccgc tgtcaatata
     1681 ggccccaaat ccgttttccg cggttacaac cgggcgctcc ttaagagcat aggcagtgat
     1741 ctctccgtcc acaacctgtg cggccagata gatctcatcc ggacaaaccc acttttcacc
     1801 gcgcatatgc ataatcggac ttttcagcat atacagatag ctgtttccaa aatttaaggt
     1861 cttcagtttc agcattgcat tataaagcat ttccttttca ctgtctacat gattgaccat
     1921 atccctggaa atcaaagaca taaaacaggc gctgcgttca tattcgctaa gagaatcctt
     1981 tgccttttta taaatatggg cctgcagata tttcatccct tctatcagta tttccgaaaa
     2041 aagcacttct gccatatgat catcaaccgt atggatcaca aaagataaat aatccgaaaa
     2101 catcgtagcc agggcggacg gtgaaatata atccgcatac ggacccgtca tcagtatacg
     2161 cagctgctcc acaacacgct tggcagacaa aatctctttt acatatccta aatagatgat
     2221 cgtattcgca tagataacat cataaagata gtcgtaaact ttatctccga tcgcatcgtt
     2281 tacatctgaa atcaatattg catctttgat cgtctctgca gttttgattg cgtaaaattc
     2341 cggattaagc ttttcggaag ccgttatttc tgaaaattta tatttttcca tatcctgaca
     2401 tccgcaggaa ttacggcgga tcagctgcgg cggcaaaata atattttcac agatcccttc
     2461 atttaaaata cgaagtacat taaacaacgc cttatatccg ctggagtagg cattctgcaa
     2521 tactgtggta agcggcggat cgagcgtggc tgccagcacc cagtcgtcat aacctgttac
     2581 cgcaacaccc tctcccctgt cgatatccct gccgatcctg taacgaagag gactcttttt
     2641 cagccattcc ctcgctttcg gggtcatctg atagatttcc agccttcgtt ttttacatac
     2701 cttataaacg gaattggcca tcaggtcatt tgcacacaca aatgcctgca catgcggatt
     2761 cagatcaaac agctcttcaa cttctttttc aacagactca gagaaatctc cgacacggat
     2821 catatccttt gttactttga tattctgctc c
//
LOCUS       DAOSYE010000236         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26433_length_2847_cov_3.353152, whole genome shotgun sequence.
ACCESSION   DAOSYE010000236 DAOSYE010000000
VERSION     DAOSYE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26433_length_2847_cov_3.353152"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLU43_05070"
     CDS             complement(<1..867)
                     /locus_tag="PLU43_05070"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOO27813.1"
                     /translation="MEALKIGELTAPIPVVQGGMGVGISMSGLASAVAAAGGIGTISA
                     AQIGFADAEFEKAPVETNLRVLKEEIKKAKKKAGDGIIAVNIMAATRFYERYVLAALE
                     AEADMIVSGAGLPMALPELAAGSAAKLVPIVSSVKSADVICRYWAKKYRRGPDMIIIE
                     GPLAGGHLGFGSEQLQHIDEMDYDTEIVSIIHKVEEYEKSFEKKIPVVIAGGIYERKD
                     LEHACSLGASGVQIGTRFVTTFECDASQAYKEAYLQAEKKDILIVKSPVGMPGRAIRN
                     KFVDRTQLGRIPH"
     gene            complement(1045..1308)
                     /locus_tag="PLU43_05075"
     CDS             complement(1045..1308)
                     /locus_tag="PLU43_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO27814.1"
                     /translation="MVSQKVVIKNPTGLHLRPAGILCKEAMQFKSLITFNFRENTANA
                     KSVLSVLGACVKCGDEVEFVCEGEDEQEALKTLITAIEGGLGE"
     gene            complement(1470..2819)
                     /gene="glmM"
                     /locus_tag="PLU43_05080"
     CDS             complement(1470..2819)
                     /gene="glmM"
                     /locus_tag="PLU43_05080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO27815.1"
                     /translation="MGRLFGTDGVRGVANTELTPQLALQLGQAGAYILTKHNSHRPTI
                     MVGCDTRISGDMLANAMMAGICSVGANAVFVGVLPTPAVAYLTRKYKVDAGVVISASH
                     NPVEFNGIKFFNGEGYKLPDAIEDEIEAIIRSDMCLLPVPIGSGVGKVKYRTDAREEY
                     INHAMKAVGMELHGMKIVVDCAEGASYYTSVETLKELGAEVIAIHNNPDGTNINANCG
                     STHLEELMARVVYEHAAVGLAFDGDADRLLAVDENGNRVDGDEIMAIIGYFMKKNKKL
                     KNDTIVATVMSNLGFFLMGEEKGIKIEKTKVGDRYVLERMREIGANLGGEQSGHIIFL
                     DENTTGDGLLSALHLLEVMVESKKPLSELKEIMEVLPQALVNAKVPNHKKETFMDYPE
                     IANAIELLEREFDGKGRVLIRPSGTEPFVRVMIEGKDQRQIDMEARRLAELIQNVML"
BASE COUNT          713 a          783 c          479 g          872 t
ORIGIN      
        1 atgtgggatc cgtccaagct gtgtcctgtc gacaaattta tttctgatcg cacggcccgg
       61 cattccaacc gggcttttca caatcagaat atcctttttc tccgcctgca aataggcttc
      121 cttatacgcc tgagaggcat cacattcgaa agtagtcaca aatctggtgc cgatctgcac
      181 cccgcttgca cctaaggaac atgcatgctc caagtccttt ctttcgtaaa ttccacccgc
      241 aatgacaaca ggaatttttt tctcaaagct tttttcatat tcttccactt tatgaatgat
      301 cgatacgatt tccgtatcat aatccatttc atcaatatgc tgaagctgtt cgctcccaaa
      361 cccgagatgc ccgcctgcca atggaccttc aataatgatc atatcagggc ctctccggta
      421 tttttttgcc cagtacctgc agatcacatc ggctgatttt accgacgaca cgatcggaac
      481 aagctttgca gcagaaccgg ccgcaagctc cggcagtgcc atcggaagac ctgctcctga
      541 aacgatcata tccgcttctg cttccagggc cgccagcaca tatctttcat aaaacctggt
      601 tgctgccatg atattaacag caatgatccc gtctcctgct ttcttttttg cctttttgat
      661 ttcctctttt aaaacacgca gatttgtttc gaccggtgct ttttcaaatt ctgcatccgc
      721 gaatccgatc tgtgccgctg aaatagtacc gattcccccg gctgccgcaa cggcggatgc
      781 cagacctgac atactgattc caacacccat gcctccctgc actacaggaa tcggggctgt
      841 cagttctcca atttttaatg cctccaaatt tcatatcctc cttaacggat aacaataaat
      901 actttgataa tcaaactata tgatcatccc tctgttttgt caacattttt ataaagaaga
      961 gaacttcatc caatcatatg caataacaaa agaaccggca aaataccggt tcttttatcg
     1021 tacgcactat atgtaagctg tccgctattc acccaggccg ccttcaatcg ccgtgatcag
     1081 tgtttttaaa gcttcctgct catcttcccc ttcacagacg aattcaactt cgtcaccgca
     1141 ctttacacag gcgcccaaaa cgcttagtac gcttttggca tttgccgtat tttcgcggaa
     1201 attaaaagta atcaaagact taaactgcat agcttccttg caaaggattc cagctggtct
     1261 taaatgcaaa cctgtaggat ttttgataac aaccttctgg cttaccatat ctaacgacct
     1321 ccattcattg tcaatattca aatattgggt atcctgtcac cggagttcac cagaaatcgc
     1381 acccgctatt tctattatat cattatttgt acaaaatcac aaccattttg tgtattttta
     1441 tgcttttttt atacaattta acaatattct tataacatga cattctgaat cagttcggca
     1501 agccttctcg cttccatatc gatctgtctt tgatctttgc cctctatcat gacccggaca
     1561 aacggttctg ttccggatgg ccgaataagc actcttccct ttccatcaaa ttctctttca
     1621 agcaattcga tcgcatttgc aatttccgga taatccataa atgtttcttt tttatgattt
     1681 ggtacctttg cattgacaag tgcctgcggc aatacttcca taatttcctt cagttcagaa
     1741 agcggtttct tgctctctac cataacctcc aaaagatgca gggcagacag cagtccatcc
     1801 cctgttgtat tctcatccag aaaaataata tgaccggact gttccccgcc taaatttgct
     1861 ccgatctcac gcatccgttc cagtacatat ctgtcgccga cctttgtttt ttcgatcttg
     1921 attcctttct cctctcccat caggaaaaac ccaagattac tcatcacggt cgctacaatt
     1981 gtatcatttt tcaatttttt attttttttc ataaagtagc cgataatagc cataatttca
     2041 tcaccgtcta ctctgtttcc attctcatcg actgcaagaa gcctgtcggc gtctccgtca
     2101 aatgccagtc caactgccgc atgctcataa acaactctcg ccatcagctc ctccagatgc
     2161 gtcgaaccgc agtttgcatt gatatttgtt ccgtcaggat tattatggat cgctatcact
     2221 tctgcgccaa gctccttcag cgtttcaaca gaagtatagt aggaggctcc ttccgcacag
     2281 tcgactacga tcttcatacc gtgaagttcc ataccgacag ctttcatcgc atggttgata
     2341 tactcttctc tcgcatccgt acggtatttc acttttccga caccggaacc gatcggaacc
     2401 ggaagcaggc acatgtcgga tctgatgatc gcttctattt catcttcgat cgcatccgga
     2461 agcttatagc cttctccgtt aaaaaatttg attccgttaa attccaccgg attatgagat
     2521 gcagaaatca caacacccgc atcaacttta tattttcttg tcaggtatgc aacggcaggc
     2581 gtcggcagta ccccgacaaa aaccgcattc gcacctacgg aacagatccc cgccatcatt
     2641 gcattcgcaa gcatatctcc ggaaatcctc gtatcacagc caaccatgat cgtcggcctg
     2701 tgggaattgt gctttgtaag tatataagca cctgcctggc caagctgcaa tgccagctgc
     2761 ggtgttaatt ccgtatttgc tactcctctg actccatcag ttccaaataa tctgcccatt
     2821 tttattcctc tcctgttgtt tctgtat
//
LOCUS       DAOSYE010000237         2837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26548_length_2837_cov_3.346873, whole genome shotgun sequence.
ACCESSION   DAOSYE010000237 DAOSYE010000000
VERSION     DAOSYE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26548_length_2837_cov_3.346873"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLU43_05085"
     CDS             complement(<1..704)
                     /locus_tag="PLU43_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO27816.1"
                     /translation="MVLLWREMWTDREEYVTYSEFMESLDDDQIMSINIVQNSEVPTG
                     VVEMMMRTGEEKYLYVPDVSEIADILKVKFPAYSMEDVKRDNWFMSYMLPSLLIFGIV
                     ALVFMYMNNQAGAAGSGGKVMNFGKSRAKLSSDDNKMTFKDVAGLEEEKDELKEIVDF
                     LKSPKKYIQVGARIPKGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGV
                     GASRVRDLFAEAKKNSP"
     gene            complement(797..1321)
                     /gene="hpt"
                     /locus_tag="PLU43_05090"
     CDS             complement(797..1321)
                     /gene="hpt"
                     /locus_tag="PLU43_05090"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOO27817.1"
                     /translation="MSEEIRVLLSEQQVDRRIQEIGEKISKDYKGRSVHLVCVLKGGT
                     FFLCELAKRISIPVSIDFMSVSSYGNETKSSGVVRIVKDLDEPLKDKNVIVVEDIIDS
                     GRTLSYLLSILKERGPASLALCTLLDKPERRVVDVAVDYTGFEIPDEFVVGYGLDYAQ
                     KYRNLPYIGVVEQN"
     gene            complement(1314..2726)
                     /gene="tilS"
                     /locus_tag="PLU43_05095"
     CDS             complement(1314..2726)
                     /gene="tilS"
                     /locus_tag="PLU43_05095"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOO27818.1"
                     /translation="MDRTYELTERVWKYIRQHKMIVCRDTVIAGVSGGADSVCLLFLL
                     KKMQEKLNFTLLAVHVEHGIRGAASVQDERIVVKLCEELSVPLTIYHKNVRALAEKEK
                     LSIEEAGRLARYGAFSEAAAGVPFAKIAVAHHMNDQAETVLFHMARGSGLKGMGGMEP
                     VRGRIIRPLLCVKRSEIEQYLKENGIRYCTDATNQELIYSRNVIRAKAVPALEEVQEP
                     AVLHIVQAAEEIREAEAYIAHQAEKLYAAAVTKDGAGYLVSAKAFLQEEPIIGRNVLK
                     MILSQLLHEWKDITRTHMGGIAALFEKPAGKEICLPKGIAAVRREAHVYIGPQAKKKE
                     EAPYFEKRLVKPDGEIWLDDGSCIKCSVCDCEKVEDIPRNTYTKWFDYDKINNGLFVR
                     TREPKDYLCINRELSKQKLKEYFINQKIPRETRDKVLLLAVENQVVWVIGHRISEQFK
                     ITEQTKRVLKVQIIGGKYHE"
BASE COUNT          739 a          816 c          466 g          816 t
ORIGIN      
        1 ggtgaatttt tcttcgcctc ggcaaacaga tcccttacac gcgaagcacc aacaccgaca
       61 aacatctcta caaaatcgga accggaaata ctgaaaaagg gaacgcccgc ttctcccgca
      121 accgctttgg caagcaatgt cttacctgtt ccgggcggtc cttccaaaag cacacccttt
      181 ggaatcctgg ctcctacctg aatatacttt ttaggactct tcagaaaatc cacgatttcc
      241 ttcaattcat ccttttcctc ttcaagtcct gcaacatcct taaatgtcat cttattatca
      301 tccgaggaga gctttgccct gctctttcca aaattcatga ccttgccgcc gctgccggca
      361 gcaccggcct gattgttcat atacataaat acaagcgcta cgatgccaaa gatcagaaga
      421 ctcggcagca tgtagctcat aaaccagtta tcccgcttca catcctccat ggaatacgca
      481 gggaacttta cctttaaaat atccgctatc tcactgacat ccggtacata gagatatttt
      541 tcctccccgg tacgcatcat catttcaacg actccggtcg ggacctcaga attctgtacg
      601 atattaatgg acatgatctg atcgtcatcc agactttcca taaattccga ataggtaaca
      661 tattcctctc tgtctgtcca catttcccgc cataaaagta ccataaaaaa gaaaagggca
      721 atcaataata tataaaaacc aagtcctttg tttcctttgc ttatcttatt cacaacagac
      781 ctcctgtctt acggtactaa ttttgttcca caacaccgat atatggaaga tttctatatt
      841 tttgcgcata atccagacca tatccgacta caaattcatc cgggatctca aatccggtat
      901 aatccacagc cacatccacg acccggcgtt ccggcttgtc aagcaatgta caaagcgcaa
      961 gacttgcagg accgcgctcc tttaaaatgc tgagcagata actcagcgtc ctgccggaat
     1021 caataatatc ttcgacaaca attacatttt tatctttcag cggctcatcc agatccttca
     1081 cgattcgcac gacaccgctc gatttcgtct cgtttccata gctggaaacc gacataaaat
     1141 caattgatac gggaatcgaa atccttttgg ccagctcgca taaaaagaac gttccaccct
     1201 ttaaaacaca tacaagatgt acgcttctcc ctttataatc cttactgatc ttctctccga
     1261 tctcctgaat cctccggtcg acctgctgtt cgctcaacag aaccctgatc tcttcactca
     1321 tgatactttc ctccgataat ttgtactttt aaaacacgct ttgtctgctc cgttattttg
     1381 aactgctcgc tgatccggtg cccaatgacc cataccacct gattttcaac agcaagaagc
     1441 agtactttat cccgtgtttc cctgggtatt ttctgattga tgaaatactc tttcagcttc
     1501 tgttttgaaa gctccctgtt aatacaaaga tagtcctttg gttcacgggt ccttacaaac
     1561 agaccattgt ttattttatc ataatcaaac catttcgtat aggtatttcg gggaatatct
     1621 tcgacttttt cgcaatcgca gacactgcat tttatgcagc ttccgtcatc cagccatatt
     1681 tccccgtcag gttttaccaa acgcttttca aaatacggcg cctcctcctt ttttttcgcc
     1741 tgcggaccga tatatacatg tgcttcacgc ctgaccgcag caattccttt cggaaggcag
     1801 atctcttttc cggccggctt ctcaaataac gcggcaattc ctcccatatg tgttcttgtg
     1861 atatccttcc attcatgcag cagctgcgat aaaatcattt tcaaaacatt tcgtccgata
     1921 atcggttcct cctgaagaaa agcttttgca gaaaccaaat aaccggctcc gtcctttgta
     1981 acagccgcag cataaagctt ttctgcctga tgggcaatat aggcttccgc ttccctgatc
     2041 tcttccgctg cctgtacgat atgcaaaacc gccggctcct gaacttcttc cagtgccggt
     2101 actgctttcg cccgtataac attgcgggaa tagatcagct cctgatttgt cgcatccgta
     2161 caataccgga tcccgttctc cttcaaatat tgctcgatct cacttctttt tacacaaagg
     2221 agcggccgaa tgatccttcc ccttaccggc tccattcctc ccatgccctt cagtccgctg
     2281 ccgcgggcca tatgaaagag caccgtttcc gcctgatcgt tcatatgatg cgcaaccgcg
     2341 atctttgcaa aaggaacacc tgctgccgcc tctgaaaacg ctccataccg ggcaagcctt
     2401 cccgcctctt ctatggaaag cttttctttc tccgccagtg cccgtacatt tttatgatat
     2461 attgttaaag gaaccgacag ctcttcacac aatttaacaa cgatcctttc atcctgtacg
     2521 gatgccgcgc ctctgatccc atgctccaca tgcacggcca gcagtgtaaa atttagtttt
     2581 tcctgcattt ttttcagcag aaacaaaagg cagacagaat ctgcccctcc tgacacccct
     2641 gcaattaccg tatccctgca cacgatcatc ttatgctgtc gaatatactt ccatactctt
     2701 tccgtcagtt catatgtcct gtccatttat cgttatcccg cacactttcc caaaatatcg
     2761 gctatgccac ctttactata gtaaataaaa acataaaatg caagcagaca ttccgtcaaa
     2821 aaaacacaga tgaacca
//
LOCUS       DAOSYE010000238         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26653_length_2830_cov_4.108829, whole genome shotgun sequence.
ACCESSION   DAOSYE010000238 DAOSYE010000000
VERSION     DAOSYE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26653_length_2830_cov_4.108829"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLU43_05100"
     CDS             complement(<1..621)
                     /locus_tag="PLU43_05100"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOO27819.1"
                     /translation="MSVGMKEKIKYILPVLLTMVLGSIVLAVKKMFPFGNDTIDYYDM
                     AQQIAAFYYHVYDMLHGEKAFFFDWYTALGVNMAMSTSGCSNLSPFNLFFLFVPRDML
                     LESLSIFNLLKMMCMTFTMYLFLHKTYRAPYFFKALLSTGYGFCGFVLVLYITNQWMD
                     IAVLFPLILLSLGKLLREGTMRWYVVTMSLALINSYYLAFMILIFIF"
     gene            complement(677..1021)
                     /locus_tag="PLU43_05105"
     CDS             complement(677..1021)
                     /locus_tag="PLU43_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO27820.1"
                     /translation="MNKELLFVMIFLLSVFISSVSQLLLKISANQSHRSGLAEYLNVR
                     VITAYSLFFGATLITVLAYRYVPLSLGQVLESTGYVFVTLLGYFVLKEKIGGQKLVGM
                     LVIIAGVLLFYL"
     gene            complement(1014..1379)
                     /locus_tag="PLU43_05110"
     CDS             complement(1014..1379)
                     /locus_tag="PLU43_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOO27821.1"
                     /translation="MHKPNLKKELIKPLILLHLIVLLYSGSAVCSKLAAGERFLSSSF
                     ILLYGTVLLLLFIYAVLWQQVLKKMPLTTAYANKAVSIIWGLVWGGVFFSETITLKKI
                     IASVMIMIGVGLVVTCRDE"
     gene            complement(1372..2151)
                     /locus_tag="PLU43_05115"
     CDS             complement(1372..2151)
                     /locus_tag="PLU43_05115"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOO27822.1"
                     /translation="MCLIINADDFGKNESVNKAVRECFEKKLIQRTTLMVNMPAADEA
                     VAIAKAAGFSDRVGIHLNLTEGRPLTGEIRNNPLLCDEYGNFHAGFQKTTKYRLYMDE
                     LTIGQIREELDAQLCKYRTYGLTLNHIDSHHHVHTNYPILRALKPLAPKYGFSSVRLS
                     RNLYHGGSLPMRLYKSLYNHAIRKLCKTGTDYFGSYQDMIAFFSVPQNEGADRKDALA
                     RLDRFLAGHSLEIMVHPMYDASGVLLDTDIPMEREKELLHA"
     gene            complement(2179..>2830)
                     /locus_tag="PLU43_05120"
     CDS             complement(2179..>2830)
                     /locus_tag="PLU43_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOO27823.1"
                     /translation="AFINELAMAFDKMGIDTAEVIEAMNTKWNALGFRPGLVGGHCIG
                     VDPYYFIYEAEKLGYHSRLISAGRQINDDMPEFVGDNIIKQLVLANKQVKQAKIVFFG
                     VTFKENCPDVRNSKIMEIVKYLEQYEIEPILIDPMADREEVEHIYAHTLSGEGLAYDA
                     DCIVLSVAHEEFMGRSIEQWDQFFKPCDAKEKVLIDIKSVLDRKQMIERGYHYWRL"
BASE COUNT          819 a          721 c          541 g          749 t
ORIGIN      
        1 aaaaataaag atcaatatca taaatgcgag ataatagctg ttgatcaggg ccagtgacat
       61 cgtaaccaca taccagcgca tggttccttc ccgcaaaagc tttccaagcg acagaaggat
      121 cagcggaaaa agaaccgcaa tatccatcca ttggtttgta atgtacagaa caagtacaaa
      181 cccgcagaaa ccatagcctg tgctcagcag tgctttaaaa aaataaggtg cacggtacgt
      241 tttatgcaaa aacagataca tcgtaaaggt catacacatc atcttgagca gattgaagat
      301 cgaaaggctc tcaagcagca tatccctcgg tacaaataag aaaaagagat tgaacgggct
      361 taaattgctg catcctgatg tactcatcgc catattgacg ccaagcgccg tgtaccagtc
      421 aaaaaagaat gccttttccc cgtgcagcat atcgtaaacg tgataataaa aagcagcgat
      481 ctgctgtgcc atatcatagt aatcgatcgt atcatttcca aatggaaaca tcttctttac
      541 agccagaaca atgcttccga gcaccatcgt caaaagcacc ggcaatatat atttgatctt
      601 ttccttcatg ccgaccgaca taatttctac tccgtttatt ccaccggtat cctttaaacc
      661 gataaagtat ggttttctat aaataaaaga gcaatactcc cgctatgatg acaagcatcc
      721 cgaccagctt ctgtccgccg atcttttctt tcagcacaaa ataaccgagc agcgtcacaa
      781 acacatatcc tgtcgattcc agcacctgcc cgagcgataa aggtacatac cgatatgcca
      841 gtaccgtaat gagcgtcgca ccgaaaaaca aactatatgc cgtgatcacc ctgacattca
      901 aatattccgc aagtcccgac cgatgactct gattcgcact gatttttaaa agcagctgtg
      961 aaacagaaga aataaatacg gataacagaa agatcatgac aaaaagcaat tccttattca
     1021 tcccggcatg tcaccaccaa tccgacgccg atcatgatca taacagaggc gattattttt
     1081 tttaatgtga ttgtttccga aaaaaacacg cctccccata caagacccca tataattgaa
     1141 acagccttgt ttgcataggc ggtcgtcagc ggcattttct tcagtacctg ctgccataaa
     1201 accgcataga taaacagaag aagcagcact gtcccatata ataaaataaa agaggaactc
     1261 aaaaaccgct ccccggccgc cagcttggaa cagacggcgc tgccggaata cagcagcacg
     1321 atcaggtgca gcaggatcag tggtttgatc agctcttttt ttaaattcgg tttatgcatg
     1381 cagtaattcc ttttccctct ccatcggaat atccgtatcc agcagcacgc cggacgcatc
     1441 atacatcgga tggaccatga tctccagact gtgccctgca agaaaacgat ccagccttgc
     1501 caaagcatcc ttcctgtcgg caccctcatt ctgcggaact gaaaaaaaag cgatcatgtc
     1561 ctgataggag ccaaaataat cagtccctgt tttgcacagt ttcctgatcg catgattata
     1621 cagactttta taaagccgca tcggaaggct tcctccatgg tacaggttac ggctcagcct
     1681 gacggacgaa aagccatatt ttggggcaag cggttttaat gcacgtaata tcgggtaatt
     1741 tgtatgtaca tgatgatgag aatcgatatg attcagtgtc aatccatagg tacggtattt
     1801 gcaaagctgt gcatccagct cttcgcgtat ctgcccgatc gtcagttcat ccatgtaaag
     1861 acggtatttt gttgtctttt gaaaaccggc atgaaaattc ccgtattcat cgcagagcaa
     1921 tggattattt ctgatttccc cggtcagcgg tctgccctcg gtcagattca gatggatacc
     1981 gaccctgtcc gaaaatccgg ctgcctttgc gatcgccacc gcttcatcgg ctgccggcat
     2041 atttaccatc aacgtcgtac gctgtatcag ttttttttca aagcactccc tgaccgcctt
     2101 attcaccgat tcattttttc cgaaatcatc cgcattaata atcagacaca ttgtttttgt
     2161 tccttttact tagggccgtt acagtctcca gtaatgatat ccccgttcga tcatctgctt
     2221 tctgtcaaga accgatttga tgtcgatcag caccttttcc ttcgcatcac acggtttaaa
     2281 aaactgatcc cactgttcga tactgcggcc cataaattcc tcatgtgcca ccgaaagcac
     2341 gatacaatcc gcatcatacg caaggccctc gccggaaagc gtgtgtgcat aaatatgttc
     2401 cacttcctcc cggtctgcca tcggatcgat caggatcggt tcgatttcat actgttccag
     2461 atattttacg atctccataa tttttgaatt tctgacatcc ggacaatttt ccttgaaggt
     2521 tacgccgaaa aatacgattt ttgcctgctt gacctgtttg tttgcaagaa caagctgttt
     2581 gataatatta tcaccgacaa actccggcat gtcgtcgttg atctgtctgc cggcggaaat
     2641 caaacgggaa tgatatccaa gcttttccgc ctcataaata aaataatacg gatctactcc
     2701 gatacagtgt ccgccgacaa gtcccgggcg gaaaccaaga gcgttccatt ttgtattcat
     2761 tgcctctatc acttctgccg tatcaatgcc cattttgtca aatgccattg ccagctcatt
     2821 gataaaggcg
//
LOCUS       DAOSYE010000239         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26655_length_2830_cov_3.789550, whole genome shotgun sequence.
ACCESSION   DAOSYE010000239 DAOSYE010000000
VERSION     DAOSYE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26655_length_2830_cov_3.789550"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /gene="lepB"
                     /locus_tag="PLU43_05125"
     CDS             <1..465
                     /gene="lepB"
                     /locus_tag="PLU43_05125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO27824.1"
                     /translation="VLVYSIGIRTSVVGTSMEPALHNGQEVLIDKLIYQFSSPKRGDV
                     IVFRPNGNQNSHFYVKRIVGLPGETIQIKQGQVYINGEVYTDDAADDTKEAGIAETEI
                     LLGDDEYFVLGDNRDNSEDSRSANIGNVGRDMIEGKAWYHLKSGEEKMGRIY"
     gene            469..1329
                     /gene="ylqF"
                     /locus_tag="PLU43_05130"
     CDS             469..1329
                     /gene="ylqF"
                     /locus_tag="PLU43_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOO27825.1"
                     /translation="MNYQWYPGHMTKARRMMQDNMKLIDLIIELVDARVPSSSRNPDI
                     DTLGNGKSRLILLNKADLADESCNHIWEDYFKGCGYTVVTVNSKTGSGVKSIYGAIQE
                     ACKEKIARDRKRGILNRPVRAMVVGIPNVGKSTFINTLAGKACTKTGNKPGVTKGKQW
                     IRLNKNVELLDTPGILWPRFESPKVGLRLAMIGSMNDEILDVEELAGELIVYLRRIKP
                     GILTGRYGILEDGTVYEVLEKIAEARHCLLKGDKKDVHKAAAILMDEYRNGKLGRVTL
                     ELPAEEGEQD"
     gene            1333..1887
                     /gene="lepB"
                     /locus_tag="PLU43_05135"
     CDS             1333..1887
                     /gene="lepB"
                     /locus_tag="PLU43_05135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO27826.1"
                     /translation="MRANKKSSLLFRFLKKAIDIGLFILSVLVLTYLTVTYVAQRTIV
                     HNVSMQETLSEGDNLIMDKISYHFREPKRFEIICFRCAGEKDDLIKRIIGLPGETVQI
                     KNGEIFIDGELLTDTAGLQAPEFAGRAENMITLGSDEYFVLGDNRSESIDSRFDEVGN
                     VRKSTIAGRAFFRIYPFDKFGLIN"
     gene            1904..2662
                     /locus_tag="PLU43_05140"
     CDS             1904..2662
                     /locus_tag="PLU43_05140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOO27827.1"
                     /translation="MTKKTGEIREEYQAASIKMLPSFIAEYRTDSRSGVSALVAKAEK
                     KLTAYAQECARTEKMKVYENKYGEYGAICGIDEVGRGPLAGPVVAGAVILPADCDILY
                     LNDSKKLTEKKREELYSVIMEKASAVGLGFISPERIDEINILQATYEAMRQAVQKLSV
                     IPDILLNDAVTIPLLDIKQVPIIKGDAKSISIAAASIVAKVTRDRLMTEYESRYPEYG
                     FAANKGYGSAAHIAALKKYGPSPIHRKTFITHFI"
BASE COUNT          881 a          514 c          757 g          678 t
ORIGIN      
        1 gtgcttgtgt attcgattgg gatccgtacg agcgttgtgg gcacatccat ggaacctgcg
       61 cttcataatg gacaggaagt gctgatcgat aagctgattt atcagttttc ttcaccaaaa
      121 cgcggagatg tgatcgtctt tcgtcctaac ggcaatcaga attcacattt ttatgtaaag
      181 agaattgtcg gactgccggg tgaaacgatc cagatcaagc aggggcaggt ttatattaac
      241 ggagaggtct atacagatga tgcggcagat gatacaaaag aagcgggtat tgcggaaacg
      301 gagattctgc tcggcgatga cgagtatttt gtactggggg ataacaggga caacagtgag
      361 gatagccgga gcgccaatat cgggaatgtc ggccgtgata tgatagaggg aaaagcctgg
      421 tatcatctga aaagcggtga agaaaaaatg ggaaggatat actgattgat gaattatcaa
      481 tggtaccctg gtcatatgac gaaagccaga cgtatgatgc aggataatat gaaattaata
      541 gatctgatta ttgagcttgt agatgcaagg gttccgtcaa gcagccggaa tccggatatt
      601 gatacgcttg gaaacggaaa gtcccgtctg atcctgttga acaaagcgga tcttgcagat
      661 gaaagctgca accatatatg ggaggattat tttaaaggct gtggttatac ggtcgttacg
      721 gtcaattcca aaaccggaag cggagtgaaa agcatttacg gagccataca ggaagcctgt
      781 aaagaaaaaa tagccagaga cagaaaacgg ggtatcctga acagaccggt cagggcaatg
      841 gtagttggga tccccaatgt cggaaagtct acgtttatta atacacttgc cggaaaagca
      901 tgtacaaaga ccggaaataa gccgggtgtt acaaaaggaa agcagtggat caggctgaat
      961 aaaaatgtgg agcttttaga tacgccgggt attttatggc cccggtttga aagtccgaag
     1021 gtcggactga gacttgccat gatcggttcc atgaatgatg aaatactgga tgttgaagaa
     1081 cttgccggag agctgatcgt ttatcttagg aggatcaagc cgggcatcct tacgggcaga
     1141 tatgggatct tagaggacgg caccgtatat gaagtgcttg aaaaaatagc agaagcaaga
     1201 cactgtcttc ttaagggaga caaaaaggat gttcataagg cggccgctat tttaatggat
     1261 gaatacagaa atgggaagct tggaagagtt acgctggagc ttccggctga ggaaggcgaa
     1321 caggactgag taatgcgtgc aaataaaaag agctccttgt tattccgatt tttaaaaaaa
     1381 gcaatcgata tcgggctgtt tattttaagc gtgctcgttc tgacatatct gacagtgact
     1441 tatgtggcac agcgtacgat cgtccataat gtttcgatgc aggaaacgct ttctgaaggc
     1501 gacaacctga tcatggataa aatatcttac catttcaggg agccaaagcg ttttgagatc
     1561 atctgtttcc gatgtgcggg agaaaaagac gatctgatca aacggatcat cggactgccg
     1621 ggtgaaaccg ttcagataaa aaacggggaa atatttattg acggagaact gcttaccgat
     1681 acggcaggac tgcaggctcc ggaatttgcc ggcagagccg aaaatatgat cacgcttggc
     1741 agtgatgaat attttgtatt gggagataat cggagtgaaa gtattgacag tcgttttgat
     1801 gaggttggaa atgtaagaaa aagtacgatt gccggcagag cttttttcag aatatatcct
     1861 ttcgataaat ttggactcat aaattaaaag gagcgtttaa ctgatgacaa aaaaaacagg
     1921 ggaaatcagg gaagaatatc aggcggcatc tataaaaatg ctgccttcct ttattgcaga
     1981 atatcggacg gattcccgca gcggtgtttc ggcacttgtt gcaaaggctg agaagaaatt
     2041 gaccgcatat gcacaggaat gtgccagaac ggaaaagatg aaggtttatg aaaataagta
     2101 cggagaatac ggcgccatat gcgggatcga tgaggtaggg cgcggcccgc tggccggacc
     2161 ggttgtcgcg ggcgcggtta tcctgccggc tgactgtgat attttatatt taaacgattc
     2221 caagaagctg acggaaaaga aaagggagga actctattct gtcataatgg aaaaagcctc
     2281 tgctgttggt cttgggttta tttcaccgga gaggatcgat gagatcaata ttctgcaggc
     2341 tacttatgaa gctatgcggc aggctgttca gaaattatcc gtaatacccg atatattatt
     2401 aaatgatgca gttacgattc ctctgcttga tatcaaacag gttccgatca tcaaaggaga
     2461 tgcaaagagt atttcgatcg ctgccgcaag tattgtggca aaggtgacaa gagacaggct
     2521 catgaccgaa tatgaaagcc ggtatccgga atatggcttt gctgcaaata aagggtacgg
     2581 atcggcggca catatcgcgg cactgaaaaa atatggcccg tcgccaatac acagaaaaac
     2641 attcattaca catttcattt aagatcaaaa cagtacggcg catatcagaa aagaggcgag
     2701 aacgatggca gtcaactttt caggttatca gggatatcaa aatcaaagca gtacggtcaa
     2761 tacttcttcc gctcagagca gcggccagac ggaaaacgca gaaaccagcc agctatcagt
     2821 atccgtgcaa
//
LOCUS       DAOSYE010000240         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26687_length_2828_cov_3.633970, whole genome shotgun sequence.
ACCESSION   DAOSYE010000240 DAOSYE010000000
VERSION     DAOSYE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26687_length_2828_cov_3.633970"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLU43_05145"
     CDS             <1..403
                     /locus_tag="PLU43_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO27828.1"
                     /translation="TVIITRLAGRTPVPEGEQLRLLAAHKSTMVIFLSAGLLERVREE
                     LLLGGYEKDTPCAIVYKASWPEEKTVIGTLEGLVRMAAENQVTKTALIIVGRFFCSSY
                     ELSRLYDAGFETGYRSVADVPGKEQITGETG"
     gene            400..1413
                     /locus_tag="PLU43_05150"
     CDS             400..1413
                     /locus_tag="PLU43_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HOO27829.1"
                     /translation="MKCENCAVLSFTEKGAKLNQEICVKLSAPDVKIKGYGFYKYPCE
                     GLTCFQELQPLVKELFFSCNLLVFIGAAGIAVRAIAPYLTDKTTDPAVLAIDENAKFV
                     ISLLSGHLGGANDWCRRISALLLAQAVITTATDGNHVFAVDEFAKNNHLYLPDMKMAK
                     EASARLLNGESIGFCSEFGIYVGNRTTGSPFSAACYLLPKNLTVGIGCKRGKTKEEIH
                     AFLCSVFQAHDLRIERIEQLTSIDLKKTESGIVQEAAALDVPYRTFGAGELQALAGAF
                     LESSFVEKTVGTGNVCERSAVLGAAYGKMEERKQVRLLIGKQVKDGMTIAVAERTIRE
                     FLF"
     gene            1440..2174
                     /gene="cobJ"
                     /locus_tag="PLU43_05155"
     CDS             1440..2174
                     /gene="cobJ"
                     /locus_tag="PLU43_05155"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOO27830.1"
                     /translation="MLNIVGFGPGGYEGMTIEAEKALEKSELIIGYTTYIELIRPYFP
                     KKEYMQTGMHGEADRCRLALAEAGSRMVALVCSGDSGVYGMAGLVYELAGQMEEGRAV
                     PEINVIAGVTAAVSGGAVLGAPLSNDFMTISLSDLLTPLTIIKKRICCAAESDTVLCL
                     YNPSSRKRTGYLKMACELVLEYQSPDTVCGYVQNINRNGQKTVITTLENLKEMQVDMF
                     TTVFIGNSSTRNINGKMVTARGYRYV"
     gene            2167..>2828
                     /gene="cobK"
                     /locus_tag="PLU43_05160"
     CDS             2167..>2828
                     /gene="cobK"
                     /locus_tag="PLU43_05160"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR00715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HOO27831.1"
                     /translation="MYEKVLLFAGTTEGRQLYGFLRQQGIRAVVCVTTEYGKTLLEEA
                     AQADKEQEGAAPVRICVERMDCNRMKKEIESFCPQLVVDATHPYATEATKNIIKACEL
                     TQTDLIRICRNKIERIKDPHIIYVKSMRAAVEFLSETAGTVFVTTGSKELPALTQLHD
                     YRERIYARILPDVKMLEKTLRLGIPAERILCVRGPVSQEMNEAAMRFCGASWMLTKES
                     GK"
BASE COUNT          810 a          548 c          805 g          665 t
ORIGIN      
        1 gactgttatt attacaaggc ttgccggcag aacgccggta ccggaaggcg agcagctgcg
       61 tctgcttgcc gcacataaaa gtacgatggt gatctttctg agtgcaggac ttttggaacg
      121 tgtacgggaa gaactgcttt tagggggata tgagaaagac acgccatgtg cgatcgttta
      181 taaagcttca tggccggagg aaaaaacagt gatcggaaca ctggaaggac ttgtccggat
      241 ggctgctgaa aatcaggtca cgaaaacggc attgattatt gtcggcaggt ttttttgctc
      301 gtcttatgag ctgtccagat tgtatgacgc agggtttgag accggatacc gcagtgtggc
      361 ggatgttcct ggaaaagaac agataacagg agagaccgga tgaagtgtga aaattgtgca
      421 gtactctcat ttacagaaaa aggagcaaaa ctgaatcagg agatctgcgt taaactgtct
      481 gcacctgatg tgaaaataaa gggatatgga ttttataaat atccctgtga agggcttaca
      541 tgctttcagg agctgcagcc gcttgtaaag gaactttttt ttagctgtaa cttgcttgtc
      601 tttatcgggg cggcagggat cgcagtgcgc gcgatagcgc cgtatctgac agataaaacg
      661 acggatcctg ccgtgcttgc aattgatgaa aatgcaaaat ttgtgatttc cctgctgtcc
      721 ggtcatctgg gcggagcaaa tgactggtgc cgcagaattt ccgcgctgct tttggcccaa
      781 gccgtaatca cgactgcaac cgatggaaat catgtatttg cagtcgatga atttgcaaaa
      841 aataatcacc tgtatctgcc ggatatgaaa atggcaaagg aagcctcggc aaggctttta
      901 aacggagagt caatcggttt ttgcagtgaa ttcggcattt atgtcggaaa caggacgaca
      961 ggctcgccgt tttctgctgc ctgttatctg cttccgaaaa atctgacggt tgggatcggt
     1021 tgtaagcggg gaaagacaaa agaggagatc catgcgtttc tttgcagtgt ctttcaggca
     1081 catgatctgc gtattgagcg gatcgagcag ctgacatcca tagacctgaa aaaaacagag
     1141 tccggtattg tacaggaagc tgcggcactt gacgtaccgt acagaacgtt tggagcggga
     1201 gaactgcagg cacttgccgg tgcttttttg gagtcttcat ttgtagaaaa aacggtcggc
     1261 acaggaaacg tctgtgaacg ttccgcagta cttggggccg cttatggaaa gatggaagaa
     1321 aggaaacagg tacgtctgct gatcggcaag caggtaaagg acgggatgac gatcgcagtt
     1381 gcggagagaa cgatccggga gtttttattc tgaaggagaa aatcaaagga gcggaataca
     1441 tgctgaatat cgtaggattt ggtccgggcg gttatgaagg aatgacaata gaggcagaaa
     1501 aagcgcttga aaaaagtgaa ctgatcatcg gatatacgac atatatcgaa ttgatcagac
     1561 cctattttcc aaaaaaagaa tatatgcaga caggaatgca cggggaggca gaccgctgca
     1621 gactggcact tgccgaagcc ggcagcagga tggttgcgct tgtatgcagc ggcgacagcg
     1681 gtgtttacgg aatggccgga cttgtgtatg aactggcagg acagatggag gaaggcagag
     1741 ccgtacctga aatcaatgtg atcgcaggag tgaccgcagc ggtcagcggc ggcgccgtgc
     1801 tgggagcccc gctctccaat gattttatga cgataagtct gagtgatctg ctgacaccgc
     1861 ttacaataat caaaaagcgg atctgctgtg cggctgagag tgatacggta ctttgtctgt
     1921 acaatccatc cagcagaaag cggaccggat atttaaaaat ggcctgtgaa ctggttcttg
     1981 agtatcagtc tccggatacg gtctgcggat atgtacagaa tattaaccga aatggtcaaa
     2041 aaactgtcat aacaacactc gaaaatttaa aggagatgca ggtggatatg ttcacgaccg
     2101 tatttattgg gaacagcagt acacgtaata ttaacggaaa aatggtaact gcaagagggt
     2161 atcggtatgt atgagaaagt attgttattt gccggaacga cagaaggccg gcagctttat
     2221 gggtttttga gacaacaggg tattcgtgcg gtcgtctgcg ttacgacaga gtacggaaaa
     2281 accttgcttg aggaagcagc acaggcagat aaagagcagg agggtgcggc tcctgtccgt
     2341 atctgtgtgg agcggatgga ctgtaacaga atgaaaaaag agatcgagag cttttgtccg
     2401 cagcttgtgg tcgatgcgac gcatccgtat gcaacggaag ccacaaagaa tattataaaa
     2461 gcctgtgaac tgacgcagac agatctgatc aggatatgca ggaataaaat agaacggatc
     2521 aaggacccgc atatcattta tgtaaaatca atgcgtgctg ccgtggaatt tctttcagaa
     2581 acggcaggaa ctgtttttgt gacaacagga agcaaggaac tgcccgcgct gacgcagctg
     2641 cacgattaca gggaaagaat ctatgccaga atactgcctg atgtgaagat gctggaaaaa
     2701 acgctgcgtc ttggcattcc ggcagagcgt attttatgtg tacggggtcc tgtttcacag
     2761 gaaatgaatg aggcagccat gcgtttttgc ggcgcatcct ggatgctgac aaaagaatcc
     2821 ggaaagga
//
LOCUS       DAOSYE010000241         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26765_length_2823_cov_3.004335, whole genome shotgun sequence.
ACCESSION   DAOSYE010000241 DAOSYE010000000
VERSION     DAOSYE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26765_length_2823_cov_3.004335"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLU43_05165"
     CDS             complement(<1..963)
                     /locus_tag="PLU43_05165"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HOO27832.1"
                     /translation="MNISAIINESIKQLPSVEGPIPVTEESHPFCSMQYCREPFDLQK
                     YDYIEEEFFLSGTANVYETNKNDMPVLKKQALPYKNRILVRRPSKPENFSGRVYLDIL
                     NATQGYDIEDLWHRNYLWCMENGHAYIGITSKPINVLSLKNFDYKRYGQLNWSNGELI
                     NAPAISNSPTLPGTEEGLFWDMLSQLGILLRTEKTHNCLGGYQVDYVYLTGQSQSGAY
                     LNTYIYYFDSFLYDADKKKLFDGYFNIVGALVQRTICQESIIGNLKLYPKKTRACSIP
                     YICISSEADLYLFQELLEDDLLSIKIDNKDSEYDKCRYYELPGTP"
     gene            1158..1760
                     /locus_tag="PLU43_05170"
     CDS             1158..1760
                     /locus_tag="PLU43_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOO27833.1"
                     /translation="MNKIAEKLVNWQIRKNIIKAEDRPAYVFAYETLLLRIMNFLIAV
                     AVGLLMKNLLGVLVFLAAYIPLRTYAGGYHARNSLNCTILSTIMIIIVAFVTKLSFVM
                     DTPGGVPVFFVLGIVCYAGILIMSPVADENKPLSDDEIKKYGKRAYMIGTAEAAVAAV
                     CLWAGQPEVAAVLILVLVIMAFMLLLGIFNNKRLQGEPHK"
     gene            complement(1791..2132)
                     /locus_tag="PLU43_05175"
     CDS             complement(1791..2132)
                     /locus_tag="PLU43_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO27834.1"
                     /translation="MTVQERIRSLREDNDLKQKTVANILDISQQVYSGYETGKHELPT
                     RHVMILADYYKVSTDYLLGKTNYPGRADSLNEPFIKGHTLADFLSNSVSLNMNGRKTA
                     YDYIMFLKNKR"
     gene            2313..>2823
                     /locus_tag="PLU43_05180"
     CDS             2313..>2823
                     /locus_tag="PLU43_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOO27835.1"
                     /translation="MEQNYSFNEFCEIIERLRAKDGCPWDREQTHASLRPCMTEEAAE
                     LVSSIRIYEETNDPENMIEELGDVLLQVVMHAEIAKEEQVFDINDVITTVSEKMIRRH
                     PHVFGDVKADTTEKVLNNWEDIKKKEKEGKDWIENPLREIPLELPALRRAEKVQKKID
                     RQYEHQKEMA"
BASE COUNT          829 a          509 c          670 g          815 t
ORIGIN      
        1 aggagtgccg ggcagctcgt aatatctgca cttgtcatat tcggaatctt tgttgtcgat
       61 cttaatggaa agcaggtcat cctccaggag ctcttgaaac agatacaggt ctgcttcact
      121 tgaaatgcag atataaggaa tgctgcatgc ccgtgttttc tttggataaa gtttcaaatt
      181 accaataatc gattcctggc agatcgttct ttgcacaagg gcgccgacaa tattgaaata
      241 tccgtcaaac agttttttct tgtctgcatc atataaaaag ctgtcaaaat agtaaatata
      301 agtgttcaga taagcaccgc tctggctttg gccggtaaga taaacataat cgacctgata
      361 gccgccaagg cagttatggg ttttttccgt acgcagcagt atgccgagct ggcttaacat
      421 gtcccagaac agaccctcct ccgtgccggg aagtgttgga gagtttgata tggcgggagc
      481 gtttataagc tctccgtttg accagttcag ctgaccgtaa cgtttgtaat caaaattttt
      541 cagacttaac acattaatgg gtttgcttgt gattccaata taggcatgcc cgttttccat
      601 acaccataaa tagttgcggt gccagagatc ctcgatgtca tatccctgtg ttgcatttaa
      661 aatgtcaaga tagactctgc ctgaaaagtt ttccggttta gaaggacggc ggactaatat
      721 cctgttttta taaggaagtg cctgtttttt taagaccggc atgtcgtttt tgttcgtttc
      781 atatacatta gcggttccgc ttaaaaaaaa ttcttcttct atgtagtcat atttttgcag
      841 atcaaacggc tccctgcagt attgcatgga acaaaaagga tgtgattcct ccgtaacagg
      901 aataggtcct tccacacttg gaagctgttt gatcgattca ttgataattg ccgaaatgtt
      961 cataattctg ttcctctcct gtttttgctg tggccgggtc acgtgctctg caagttaaat
     1021 agtaacattt taaaagacaa tattcaataa caactctttt gagagtatga ttgatttcaa
     1081 ttgacacaat tgggaaacta tggtaatata atgcggagta agagcaggat caaagataaa
     1141 aatacagagg gtaaaagatg aataaaatag cggaaaagct tgtaaattgg cagatcagaa
     1201 agaatataat caaagcagag gaccggccgg catatgtatt tgcctacgaa acgctgctgc
     1261 tgaggatcat gaattttctg atcgcggtag ctgtcggact tttaatgaaa aatctgctgg
     1321 gagtgcttgt gtttcttgcg gcgtatattc cgcttagaac gtatgccggg gggtatcatg
     1381 caagaaatag tctgaactgc acgatcttat caacgatcat gattataatt gttgcgtttg
     1441 ttacgaagct ttcatttgtc atggatacgc cgggaggtgt tcctgtattt tttgtgctgg
     1501 ggatcgtctg ttatgccggg atcctgatca tgtcgccggt cgcggatgaa aataaaccgc
     1561 tgagtgatga tgaaataaaa aagtatggaa aaagggcata tatgatcggt acggcggagg
     1621 ctgccgtggc agcagtctgt ctatgggccg ggcagccaga agttgcagcg gttttgatcc
     1681 ttgtattggt gatcatggca tttatgctgc tccttggtat ttttaataat aagcggctgc
     1741 agggagagcc gcataaataa taaaaacatc cgaaaggatg tttttattat ttagcgtttg
     1801 tttttcagaa acatgatgta atcatatgct gtttttctgc cgttcatatt cagggaaaca
     1861 gagtttgaca gaaaatcggc aagcgtatgg cctttgataa acggttcgtt caggctgtcg
     1921 gctcttcccg gataatttgt tttaccgaga agataatcag tagaaacttt atagtaatca
     1981 gccaagatca ttacatgccg tgtcggcagt tcgtgcttgc ctgtttcata accggaataa
     2041 acctgctgcg aaatatcaag gatgttggca actgtttttt gcttgaggtc attatcctcc
     2101 cgtaagcttc taattctttc ctgtaccgtc atgttgttca ccccacttta atgtaataag
     2161 agaataccat acaaaatcag atggtattat gccgtacaat tattaaatgt aaaatataag
     2221 tttaaacaca gaattcatgt gaatgaaaga gcagtgattg tagttttttt gttttcatga
     2281 taaagtaata gctgccagga aaggagaatc gtatggagca gaactattca tttaacgaat
     2341 tttgcgaaat tattgagaga ctgcgggcta aggacggctg tccgtgggat cgtgagcaga
     2401 cgcatgcgtc gttgagaccc tgtatgacag aggaggcagc ggagcttgtt tcttccataa
     2461 gaatatatga ggaaacgaat gatccggaaa atatgatcga agaactggga gatgtcctgc
     2521 tgcaggtagt tatgcatgcg gagatcgcaa aggaggaaca ggtttttgat atcaacgatg
     2581 tcataacgac tgtcagtgaa aaaatgatca gacggcatcc gcatgtattt ggggatgtaa
     2641 aagccgatac gacggaaaaa gtactgaata attgggaaga tataaaaaag aaagaaaaag
     2701 aggggaagga ctggatagaa aatcccctgc gggaaatccc gcttgaactg ccggcactcc
     2761 gcagggcgga aaaggttcag aaaaaaatag acagacagta tgaacaccaa aaagaaatgg
     2821 cag
//
LOCUS       DAOSYE010000242         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_26943_length_2811_cov_2.303338, whole genome shotgun sequence.
ACCESSION   DAOSYE010000242 DAOSYE010000000
VERSION     DAOSYE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26943_length_2811_cov_2.303338"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLU43_05185"
     CDS             complement(<1..192)
                     /locus_tag="PLU43_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOO27836.1"
                     /translation="MKEIKKISLIGLGAMGVFFAPRLYEKYGTDFCVIAEGERKKRLE
                     TKGVTINGINYRFPIVTLKE"
     gene            complement(189..2207)
                     /locus_tag="PLU43_05190"
     CDS             complement(189..2207)
                     /locus_tag="PLU43_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOO27837.1"
                     /translation="MIKKYRLGKPIQTDAVLTKIQAESRLPVFLQEEGSVLFSCELQK
                     DAAVYGLGEQIRGINKRGWKYVSCCTDDGVHTEEKHSMYGVHNFLVVEGEEPFGLFAD
                     YPGFMTFDIGYTHSDRLQISVTDKDLDFYYITGETVPDIIRQFRQLIGRSYIPPKWAF
                     GFGQSRWGYKDAADIRTVADGYQKNGIPLDMIYLDIDYMDHYKDFTVDEAAFPDFEKF
                     VEEMRARGIRLIPIIDAGVKIEKGYSVYEEGAANDYFCKDADGNDFVAGVWPGRVHFP
                     DMMKPETRTWFGHCYRALIEKGIEGFWNDMNEPAIFYSEKHLEEVFDRIGSYRQQNLD
                     IKTFQEFQGLVAGINNNIEDYDRFFHEINGEKTAHTKVHNIYGFQMTRAAGEAFEEME
                     PEKRLLLFSRSSYIGMHRYGGIWMGDNQSWWSHILLNLKMLPSLNMCGILYTGADIGG
                     FSGDTTEDLVIRWTEFGIFTPLMRNHTAIGTRNQEAYVFDHPEIFRDIIGLRYALIPY
                     LYSEFMKAALHDEMMFRPISFLYPKDSVAKQVEDQLFVGESIMIAPVYTQNAKGRYVY
                     LPERMKLIRFRSADRYTETVLEAGHHYVELALEEVAVFIRFHHVLPLAGPAQNMEALD
                     EKDLLLLSFTERPCEYILYHDDGYGRDYEKPAHYTTLRAKNSGERKRL"
     gene            2372..>2811
                     /locus_tag="PLU43_05195"
     CDS             2372..>2811
                     /locus_tag="PLU43_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOO27838.1"
                     /translation="MFSIKTGSLPEISYFNEIIFTDDWIHFERTADTPILYVIKSGAM
                     YIEENGIQYALKAGDMLLLETGLHHIGFDKSPCDYYYVHFSGPDIQKVTGSVISRKEF
                     LYLPKHFSVNDRQALLRIFTLFNEGIRIYEYDLEQYRLLTACKL"
BASE COUNT          712 a          744 c          529 g          826 t
ORIGIN      
        1 ctctttaagc gtaacgatcg gaaacctgta attgatccca ttgatcgtaa cgccttttgt
       61 ttcaagacgt ttttttcttt ctccctctgc gatcacgcag aaatccgttc catatttttc
      121 atataaccgc ggagcaaaaa aaacacccat tgctcccaga ccgatcaatg atattttttt
      181 tatttccttc ataatcgttt cctttcaccg ctgtttttgg ctcttaatgt cgtataatgc
      241 gccggttttt catagtctct gccataaccg tcgtcatgat aaagtatata ctcacacggc
      301 cgctctgtaa agcttagtaa cagaaggtcc ttttcatcaa gcgcttccat gttctgcgca
      361 ggtcctgcca gaggcagcac atgatgaaag cgtataaaaa ccgccacttc ttcaagcgcc
      421 agttccacat aatgatgtcc tgcttccaaa accgtctccg tatacctgtc tgccgaacga
      481 aatcggataa gcttcatccg ttccggcaga tacacatatc tccctttggc attttgtgta
      541 tagaccggtg cgatcataat actctctccg acaaacagct ggtcttcgac ctgctttgca
      601 acactgtctt ttggatataa aaaggaaatc ggtctgaaca tcatttcatc atgcagggct
      661 gctttcataa attcactgta cagataaggg atcagtgcat accgcaggcc gataatatcc
      721 ctgaaaattt ccggatgatc aaatacatac gcctcctggt tccgtgttcc gatcgccgta
      781 tggtttcgca tcagcggtgt aaaaataccg aattctgtcc atcggatcac aagatcctcc
      841 gtcgtgtctc cggaaaagcc tccgatatcc gcccctgtat aaaggattcc gcacatattc
      901 agcgacggca gcatcttcag attcagcagg atatgtgacc accaggattg attgtctccc
      961 atccagatcc ctccgtaacg gtgcatcccg atataggaag atcttgaaaa caacagcagt
     1021 cttttttccg gttccatttc ctcaaaggct tctcctgctg cccgtgtcat ctgaaaaccg
     1081 taaatattat gcactttggt atgcgccgtc ttttctccgt tgatctcatg aaaaaaacga
     1141 tcgtaatctt caatattatt gttgattccg gcaaccagtc cctgaaactc ctgaaaggtt
     1201 ttgatatcca gattttgctg ccgatagctc ccgatccggt caaatacttc ctccagatgc
     1261 ttttccgaat agaagatggc cggctcattc atatcattcc agaagccttc gatccctttc
     1321 tcaataagcg cccggtagca gtgtccgaac cacgttcttg tttccggctt catcatatca
     1381 ggaaaatgca cgcggcccgg ccatacaccg gccacaaaat cattgccgtc cgcatccttg
     1441 cagaaataat cattcgcagc gccttcttca taaaccgaat agcctttttc tattttgact
     1501 ccggcatcaa tgatcggtat cagacggatc cctcttgcgc gcatttcttc gacgaatttt
     1561 tcaaaatcag gaaatgccgc ttcatcaacg gtaaaatctt tataatgatc catatagtca
     1621 atatccagat agatcatatc aagcgggatc ccgttttttt ggtatccgtc cgcaacggtc
     1681 ctgatatccg ccgcatcctt atagccccag cggctctgtc cgaaaccaaa tgcccatttt
     1741 ggcggaatat agctcctgcc gatcagctgc ctgaactgcc tgataatatc aggtacggtc
     1801 tctcctgtaa tataatagaa gtccagatct ttatcggtta cactgatctg cagtctgtca
     1861 gaatgcgtat atccgatatc aaacgtcata aaccccggat aatcagcgaa caggccaaac
     1921 ggttcttctc cttccacgac aagaaaatta tgtacgccgt acatggaatg cttttcctct
     1981 gtgtgcacac cgtcgtccgt acagcagctc acatacttcc atccccgctt gttgataccg
     2041 cgtatctgct ctccaagccc gtacaccgcc gcatccttct gcagttcaca gctgaacagt
     2101 acgctgcctt cctcctgcag aaacacggga agtctgcttt ctgcctgaat ctttgtcaaa
     2161 actgcatctg tttgtatcgg ttttcccaat ctgtattttt ttatcatatg tttacctcgc
     2221 tttcatgttc taagccaaag tatatcggtt ctttattttc tgtacatgtt ctttggatat
     2281 aaaatttgta ttatagcgac ttgcccttct gattacgcaa tgctatactg tactgacagt
     2341 acacacacgg tcaaatacag gaggcgatcc catgtttagc attaaaaccg gcagtttacc
     2401 ggaaatatct tatttcaacg aaatcatttt taccgacgac tggatccatt ttgaaaggac
     2461 agcggataca ccgatcctct atgtgatcaa aagcggcgcc atgtatattg aggaaaacgg
     2521 gatccaatat gccctgaagg ccggtgatat gctgctcctg gaaaccggtc tgcatcatat
     2581 cggttttgat aaaagtccgt gcgattacta ttatgttcat ttttccggtc cggatattca
     2641 aaaagttacc ggcagcgtaa tttcccgcaa agaattcctt tatctgccga agcacttttc
     2701 cgtcaatgac cggcaggcgc tgcttcggat ctttactctg ttcaacgaag gcatccgtat
     2761 ttacgaatac gatctggaac agtatcggct cctgactgcc tgcaagctta t
//
LOCUS       DAOSYE010000243         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27162_length_2796_cov_2.620576, whole genome shotgun sequence.
ACCESSION   DAOSYE010000243 DAOSYE010000000
VERSION     DAOSYE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27162_length_2796_cov_2.620576"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLU43_05200"
     CDS             complement(<1..382)
                     /locus_tag="PLU43_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27839.1"
                     /translation="MKNYIKKGFLCIAGCLLSILVSGVRIRAEEPFVSVVCENGLSVL
                     IREGEPYYTNAPLYFYISEDSGETAVCEYALSSDGGQSYSDWTTADGFCYTLVPDVSN
                     SENGLWHVKFRKIIFQEEDVSQNEI"
     gene            complement(379..2358)
                     /locus_tag="PLU43_05205"
     CDS             complement(379..2358)
                     /locus_tag="PLU43_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27840.1"
                     /translation="MTETRSWKYKSCLIMAFIIIFLLIMGTCRTETPADVQTKTYTYD
                     LCSSRSSVGVTDQTSTVYLDLSGVGKISVELSYYYGKCGNCNASSGYTDASVTIAGVT
                     TARAGGSTYMEISGLEEYDLGVTPITLWCRAAINNECPVCGRCSTEIVTIKSITTYVY
                     PPAITKNPSDCQALTDGTARFSAAGTRISDYSWQKKTGEEFITLIDTVLENGTVYSGT
                     NTNQLEISGIRLSEDQSVYRCMMTADTGAVLTSNTACMTVTDVTVPTLQITMEPKTWT
                     NGPVVLTAEATDIDSGFSDMPYSWDAGLTYENAGEHQVSENGIYYVTAVDAAGNTNTA
                     SAEVTIIDRTLPTVSVSANMIEQTTAPVTIKITAEDKESGLKEQPYFYNGEWNAADAF
                     EVTCNGNYEIKAADNAGNMAELIYEVNNIKCEEDDAGNDGNEDGSGESGDSGSSQDTG
                     GSQEDGTSAVVPDSKIITPPVLLPAELSALTDRDEQKQDSDTIEEIEEEKQEMTVENE
                     KKNEQKESCETQIEVSASQDPESGDENDVIPKQKNGIFWRVFVGCLCMILALLLLFLL
                     FFGVLIEKQDPGSGKWKPCGVTVVYINKMRWCLHIGKRFDGQEVLRLRFGIVFAALFE
                     GWKLLVYTKGKESGEIREEICQNLKIFRKQIRRQM"
     gene            complement(2370..>2796)
                     /locus_tag="PLU43_05210"
     CDS             complement(2370..>2796)
                     /locus_tag="PLU43_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27841.1"
                     /translation="CFFIELCRLQTEFSEDAKSYETCLLQFYEYNRSEGFSEKKYEND
                     LMIAACYEQFADTAYGTKKAIECLETGWNELNTGKETESVISLKAEYARRLCLLYERA
                     GEDEMKRFYGELALGFLGEEETAAISDIASRIQNKEEQE"
BASE COUNT          656 a          774 c          499 g          867 t
ORIGIN      
        1 gtatttcatt ctgactgaca tcctcttcct gaaatattat ctttcggaac ttaacatgcc
       61 aaagaccatt ttctgaattc gagacatccg gaacaagtgt gtaacagaat ccatccgcag
      121 ttgtccagtc actgtagctc tgccctccat ccgaactcag cgcatactca caaactgctg
      181 tttctccgga atcctcggaa atataaaagt acagcggcgc atttgtataa tagggttctc
      241 cttccctgat cagtacggac agaccatttt cacacacaac actgacaaat ggctcctccg
      301 ccctgatcct gaccccggac acaagtatgg acaacagaca gccggcaata cataggaatc
      361 ccttttttat atagttcttc acatctgcct ccttatttgt tttctgaaaa ttttaaggtt
      421 ctgacaaatt tcttctctga tctctcccga ttcttttcct tttgtataaa ccagcagttt
      481 ccagccttca aacagtgctg caaacacgat accaaaacga agcctgagca cctcctgccc
      541 atcaaatctc tttcctatat gcagacacca tctcatttta tttatataga ccactgtcac
      601 accgcatggc ttccactttc cgcttccagg atcctgcttt tctatcagta cgccgaagaa
      661 taaaagaaac agcagcagga gtgccagaat catacaaagg catccgacaa agaccctcca
      721 aaagatccca tttttttgtt tcggtatcac gtcgttctca tctccactct ccggatcctg
      781 actggcagac acttctattt gggtctcgca tgattctttc tgttcgttct ttttctcatt
      841 ttcaacagtc atttcctgtt tttcctcttc tatttcttct atggtatccg aatcctgctt
      901 ctgttcatcc ctgtcagtca acgcggaaag ctctgccggc agcagcactg gtggagttat
      961 gatttttgaa tccggcacca cagcagaagt tccgtcttcc tgactgccgc ccgtatcctg
     1021 ggaggaaccg ctgtcgcccg actccccgct gccgtcttca ttgccgtcat tcccggcgtc
     1081 atcctcttca cacttgatat tattcacttc atagataagc tctgccatat ttcctgcgtt
     1141 atccgctgct tttatttcat agtttccgtt gcacgtaact tcaaatgcat cggccgcatt
     1201 ccattctccg ttataaaaat atggctgttc cttaagtccc gattccttgt cttcggctgt
     1261 tattttgatc gtcaccggag ccgtcgtctg ttcgatcata tttgcggaaa cagaaactgt
     1321 cggaagcgtc ctgtcaatga tcgtcacctc cgcactcgcc gtattcgtat tacctgcagc
     1381 atccactgcc gtcacataat aaataccatt ttcagacacc tgatgctctc ctgcattttc
     1441 atatgtcaga cctgcatccc atgaatacgg catatccgaa aatccgctgt cgatatcagt
     1501 tgcctctgct gtgaggacaa ccggaccatt cgtccatgtc tttggctcca ttgtaatttg
     1561 caaagtcgga acggttacgt ccgttactgt catacatgcc gtatttgacg taagaaccgc
     1621 tccggtatct gccgtcatca tacagcggta caccgattgg tcttcagaaa gccggatccc
     1681 gcttatttcc agctgattcg tatttgttcc ggaatagact gtcccgtttt ccaaaactgt
     1741 gtcgataagt gttataaact cctccccggt tttcttctgc catgaatagt cagaaattct
     1801 tgtgccggca gccgaaaatc tggctgtccc gtctgtcagc gcctggcagt ccgacggatt
     1861 ttttgtaatt gccggcggat atacgtatgt cgtaatcgac ttgatcgtaa ctatttccgt
     1921 actgcagcgt ccgcataccg gacattcgtt attgatcgca gcccggcacc acagcgttat
     1981 cggtgtaact ccaaggtcat attcttccag accggaaatc tccatatagg tacttcctcc
     2041 tgcacgtgcg gtcgttactc ctgcaatagt aactgatgcg tcggtatatc cggagcttgc
     2101 attgcaattt ccgcattttc cataataata gctcaattca acgcttattt tcccaacacc
     2161 gctcagatcg agatacaccg tggacgtctg gtcggttacc ccgacactgc tgcggctgga
     2221 acacaaatca tacgtatacg tctttgtctg tacatctgcc ggtgtttccg tccggcaggt
     2281 ccccataata agtaaaaaaa taataataaa tgccatgatc aggcagcttt tgtatttcca
     2341 cgatcttgtt tctgtcattt gttcctcctt tattcctgtt cctccttatt ttggatacgc
     2401 gatgcgatat cgctgattgc cgctgtttct tcctccccta aaaatcccaa cgcgagttct
     2461 ccgtaaaacc gcttcatttc atcttctcct gcacgttcat ataaaaggca cagccttctg
     2521 gcatattctg cctttaacga tatgactgat tccgtttcct tcccggtatt taattcattc
     2581 catcctgttt ccagacactc tattgctttt tttgtaccat acgccgtatc tgcaaactgc
     2641 tcatagcagg ccgcaatcat caggtcattt tcatattttt tttcagaaaa accttctgat
     2701 ctgttatatt cataaaactg cagaagacag gtttcataag acttggcatc ttccgaaaat
     2761 tctgtctgaa gtctgcacag ttcaataaaa aaacac
//
LOCUS       DAOSYE010000244         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27403_length_2782_cov_3.242024, whole genome shotgun sequence.
ACCESSION   DAOSYE010000244 DAOSYE010000000
VERSION     DAOSYE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27403_length_2782_cov_3.242024"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLU43_05215"
     CDS             <1..761
                     /locus_tag="PLU43_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOO27842.1"
                     /translation="GFGNEKTVAVDHVDFEFHEGEIISVVGESGSGKTTLSKMLLGLI
                     SVTEGEISFQGKVRDISSHKKKTEYWKGIQAIFQDPFSSFNVFHKIDDVLLDCIKMRG
                     GARLSYDKKIEMMTEACSFVNLKFAELTNKYPFELSGGQMQRLMIARIFLLRPKILLA
                     DEPTSMIDACSRATILDMLLKLREEINMTIIFITHDIGLAFYISDSVYIMEHGKFVES
                     GNAESVILYPKAAYTKRLISDVPKIHEEWDLSTV"
     gene            792..>2782
                     /locus_tag="PLU43_05220"
     CDS             792..>2782
                     /locus_tag="PLU43_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOO27843.1"
                     /translation="MVTETIDRNFKMRIVGEDMWIPAEVPGSVYADLLAGNRMEDPYW
                     RDNELKALEIMREDFEYIGTFAVTDDMMQQEKIFLHFDGLDTLADIKLNGVWLGSAYN
                     MHRIWEFEVSNLLKSENNEIQIVFHSPVNYIEKKYKSEPVGGSEDAMRGFPKLRKAHC
                     MFGWDWGPRLPDAGIFRGISLRGVTDARLDSVYIEQNHTLGGVLLDFHVAMKQVCNDS
                     AVSFLKEKKAALRDGYAYRVAITEPETESLSAVTVYDRSPETILIENPKLWWPNGYGE
                     QNLYGVRVELLKDGETIDIWEKRIGLRTLTVCRETDEFGESFAHEVNGVRIFAMGADY
                     IPEDNLLSRCTPERTRKLLEQCKAANYNVIRVWGGGYYPDEPFWDVCDELGLIVWQDF
                     MFACAVYDLTEEFEENIRQEFIDNIRRIRHHASLGLWCGNNEMEMFVDAGAWNCTLKQ
                     KADYIKMYEYIIPKVLKEEDPVTFYWPASPSSGGSFDCPNDENRGDVHYWDVWHGNKP
                     ITEYRKFYFRYVSEFGFQSFPSLKTVETYTEKEDRNIFSYVMEKHQRNSMANGKIMNY
                     MEQFFLYPNDFDTLLYASQLLQAEAVRYGVEHFRRNRGRCMGAIVWQLNDCWPVASWS
                     SIDYYGRWKALHYYEKRMFAPLMISCAEEGILTQDTNPNA"
BASE COUNT          811 a          502 c          743 g          726 t
ORIGIN      
        1 ttggtttcgg aaacgaaaaa acagtagcgg ttgatcatgt ggattttgaa tttcatgaag
       61 gtgagatcat ctccgtagtc ggagaaagcg gctccggaaa aaccacatta tccaaaatgc
      121 ttcttggact gatcagtgtc acggaaggtg agatcagttt tcagggaaaa gtcagggata
      181 tttcctccca taaaaagaaa acagaatatt ggaaaggaat tcaggcgatt tttcaggatc
      241 ctttttcttc ttttaatgta ttccataaaa tcgatgatgt gcttctggac tgtattaaga
      301 tgagaggagg tgcaagactt tcgtatgata aaaaaatcga gatgatgaca gaggcatgca
      361 gctttgtcaa tctgaaattt gcggagctga cgaacaaata tccgtttgag ttatcaggag
      421 gacagatgca gaggctgatg atagccagga tctttttgct cagacctaaa atcctgctgg
      481 ccgatgagcc tacgtctatg atcgatgcct gttcaagagc tacgattctc gatatgcttt
      541 tgaaattaag agaagagatc aacatgacaa ttatttttat tactcatgat atcggacttg
      601 cattttatat ttccgattcc gtttatatta tggagcatgg aaagtttgtg gagtccggaa
      661 atgcggaatc ggttatttta tatcctaagg ccgcatatac gaaaaggctc atcagtgatg
      721 tgccgaagat ccacgaggaa tgggatttat ccacggtctg agcaaagcat gaaggcaaag
      781 gagtgaacga cttggttaca gaaacaattg acagaaattt taaaatgcgg atcgtcggag
      841 aggatatgtg gataccagca gaggtcccgg gaagcgtata tgccgatctt ctggcaggga
      901 acaggatgga ggatccctac tggagagaca atgaattaaa ggcactggaa atcatgaggg
      961 aagactttga atacattggg acctttgctg tcacggacga tatgatgcag caggaaaaaa
     1021 tttttcttca ttttgatggt cttgatacac tggccgatat caaattgaat ggggtgtggc
     1081 tcggttcggc ttataacatg caccgtatat gggaatttga agtttcaaac ctgttgaaat
     1141 ctgaaaacaa tgagatccag atcgtatttc attcgcctgt caactacata gaaaaaaaat
     1201 acaaaagtga accggtcgga ggttcggagg atgcaatgcg cggatttccg aagcttagaa
     1261 aggcacattg catgttcggc tgggattggg ggccgcgtct tcccgatgcc ggtatcttta
     1321 ggggaatcag tttaagaggt gtcacagatg ccagactgga tagtgtttat atagaacaga
     1381 atcatacatt aggaggagta ctgctggatt ttcatgttgc catgaaacaa gtctgtaacg
     1441 attcggcggt ttctttcctg aaagagaaga aggctgcact aagagacggg tatgcctaca
     1501 gagtcgccat aacggaaccg gaaacagaaa gcctctcggc agtcactgtt tatgacagat
     1561 caccggaaac gattctgata gagaatccaa agctttggtg gccaaatggg tacggagagc
     1621 agaatctata tggagtccgc gtagagctgc taaaggacgg cgaaacgatc gatatatggg
     1681 agaaacgcat tggtctgcgg acgctgaccg tgtgcaggga aaccgacgaa tttggggaat
     1741 cctttgcaca tgaagtgaat ggtgtcagaa tatttgccat gggggctgat tatattccgg
     1801 aggataatct gctgagccgg tgtacaccgg agcggacaag gaagctgctg gaacagtgca
     1861 aggcagcaaa ctacaatgtc atacgcgtat ggggcggcgg atattatccg gacgagccgt
     1921 tctgggatgt ctgtgacgaa ctgggactga tcgtgtggca ggattttatg tttgcctgtg
     1981 cagtatatga tctgacggaa gaattcgaag aaaatatccg gcaggaattt atagataata
     2041 ttcgcaggat ccggcatcat gcatcccttg gactctggtg cggaaacaat gaaatggaaa
     2101 tgtttgtgga tgccggtgca tggaattgca cattgaaaca gaaggctgat tatattaaaa
     2161 tgtatgagta tatcattcca aaagtactga aggaggaaga tccggtaacc ttttactggc
     2221 cggcaagtcc ttcttccgga ggcagctttg actgtccgaa tgatgaaaac cggggcgatg
     2281 tccattactg ggatgtgtgg cacgggaata agccgatcac ggaataccgg aagttttatt
     2341 tcaggtatgt atccgaattc ggttttcagt ctttcccatc cttgaaaacg gtagaaacgt
     2401 atacggaaaa ggaagaccgc aatatttttt cctatgtaat ggaaaagcat cagaggaatt
     2461 ctatggcaaa cggaaagatc atgaattata tggaacagtt ttttttgtat ccgaatgatt
     2521 ttgacacact gctgtatgcc tcccagctgc tgcaggctga ggctgtccga tatggcgttg
     2581 agcatttcag gcggaaccgg gggaggtgca tgggtgctat cgtatggcag ttaaatgact
     2641 gctggccggt tgcttcctgg tcgtccattg actattacgg cagatggaaa gcactgcatt
     2701 attatgaaaa acgaatgttt gcgccgctga tgatctcctg cgcggaggaa ggtattttaa
     2761 cacaggacac aaatccgaat gc
//
LOCUS       DAOSYE010000245         2773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27555_length_2773_cov_3.570272, whole genome shotgun sequence.
ACCESSION   DAOSYE010000245 DAOSYE010000000
VERSION     DAOSYE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27555_length_2773_cov_3.570272"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLU43_05225"
     CDS             complement(<1..1387)
                     /locus_tag="PLU43_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27844.1"
                     /translation="MNHNAEKNPMGGKPPIHRFKPGTIKRLFSYMAQYRLSLIFVVVC
                     IFISAAASAASSLFLKTLIDDYIVPLLGQAHPEYTQLFRIILIIGSVYLIGVLSTLFY
                     NRTMVSIGQGTLKKIRDTMFEHMQSLPIRYFDTHTHGDVMSHYTNDTDTLRQAISQSL
                     PQMFSSLVTVIAAFLSMLYLSVGLTLFVGIFTIVLLRVIRALVGRSGIFFVKQQASLG
                     TVNGYIEEMINGQKVVKIFCHERSAEEEFNEKNDELCYSATEANKYGNITMPIVGNMG
                     YMLYVILAVIGGAAGIAELPNLSLTGINVLTIGTIVSFLTLSRAFINPIGQISMQFNM
                     VIMALAGASRIFELMDEEIEQDAGYVSLVNTKTANGELKECSERTDTWAWKHPHGDGT
                     TTYTPLKGEIHFYDVDFGYEENKIVLHNVTLHAEPGQKVALVGATGAGKTTITNLINR
                     FYDIADGKIRYD"
     gene            complement(1377..>2773)
                     /locus_tag="PLU43_05230"
     CDS             complement(1377..>2773)
                     /locus_tag="PLU43_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27845.1"
                     /translation="HLKCILNIRMMVRLAVRAPMMIIFALIFSFRIDAKLSLIFFAVI
                     PILALGLGLIMTHVHQIFVFVFKTYDKLNNVVQENLHGIRVVKSYNREAFEEKKFEKI
                     SQVIFENFSKAEKRLAFNMPLMQFCLYMSMLLLSWFGAKAIVSCGNDAALGLSTGELT
                     SLITYAMQILMSLMMLSMIFVMIIISRASAERIVEILEEESDLKNPEQPVHTVADGSI
                     TFENVTFSYSKEADKPVLCNINLSIASGETIGIIGGTGSAKSSLVQLIPRLYDISEGR
                     ITVGGVDVRDYDIAALRDQVAIVLQKNVLFSGTIKKNLRWGSESATEEELVHACELAQ
                     ADSFIREFPDQYNTYIEQGGTNVSGGQRQRLCIARALLKNPKILILDDSTSAVDTKTD
                     SQIRQAFQNSIPNTTKLIIAQRVSSVQDADKIIVMNDGRIEAFGTHSELLTQCSIYQE
                     VYESQTKGGMLHES"
BASE COUNT          731 a          643 c          645 g          754 t
ORIGIN      
        1 tgtcgtaacg gatctttccg tccgcaatat cgtaaaaacg gttgatcaga tttgtgatcg
       61 ttgtctttcc ggcaccggtt gcgcctacca gggctacctt ttgtcctggt tcggcatgga
      121 gggtaacatt gtgcagtacg atcttatttt cttcatagcc gaaatccaca tcatagaaat
      181 ggatctctcc ttttaacggc gtataagtag tcgtgccgtc tccatgcgga tgcttccacg
      241 cccatgtatc cgtccgttcg ctgcattcct ttaattcccc gttcgctgtt tttgtattta
      301 caagcgaaac atatccggcg tcctgttcga tctcctcatc catcagttcg aaaattcttg
      361 atgcaccggc aagagccatg atgaccatgt tgaactgcat ggatatctga ccgatcggat
      421 tgataaaggc ccgggaaagg gtcaggaaag aaacgatcgt accgatcgtg agaacattga
      481 ttcccgtaag acttaaatta ggaagttccg caatgccggc agcaccgccg atcacggcca
      541 gtatgacgta gagcatgtat cccatattgc cgactatcgg cattgtaata ttgccgtatt
      601 tgtttgcttc ggtcgcgctg tagcagagtt catcgttttt ttcattgaat tcttcttccg
      661 cacttctttc atgacagaag atctttacga ctttctgtcc attgatcatt tcttcaatat
      721 agccgttgac agtaccaaga gaggcctgct gttttacgaa aaagatgccg cttcttccaa
      781 caagtgcccg gataactctc aagaggacta tagtaaagat acctacgaac agcgtcagtc
      841 ctacactgag atacagcatg gagagaaatg ccgcgatgac agtgacaaga gaagaaaaca
      901 tctgcggcag actttgtgaa atagcctgtc tgagtgtatc ggtatcgttt gtatagtggc
      961 tcataacgtc accgtgggta tgcgtgtcaa agtaacggat cggcagagac tgcatgtgtt
     1021 caaacatcgt atcacggatc ttttttaaag tgccctgtcc gatcgatacc atcgttctgt
     1081 tataaaacag tgtggaaaga actccgatca gataaaccga gccgatgatc agaattatac
     1141 ggaacagctg tgtatattcc ggatgtgcct gtccaagcag cggtacgata taatcgtcga
     1201 tcagcgtttt gagaaacaga gaggaggctg cgctggcggc cgcacttata aaaatacaga
     1261 cgacgacaaa aatcagagaa agtctgtatt gtgccatata agaaaacagt cgtttgatcg
     1321 taccaggttt aaaacgatgt atgggaggct ttccgcccat tgggtttttc tcagcgttat
     1381 gattcatgga gcatccctcc tttcgtctgg gattcataaa cttcctgata aatggaacat
     1441 tgcgtgagca gttcactgtg tgtgccaaag gcttcgatcc ggccgtcgtt catgacaatg
     1501 atcttgtcgg catcctgtac ggaagagacc ctctgtgcaa tgatcagttt tgtagtattc
     1561 ggtatgctgt tctggaatgc ctgtctgatc tggctgtctg tttttgtatc taccgcactg
     1621 gtagagtcgt ccagaatcag gatttttgga tttttcagca gggcgcgggc aatacatagc
     1681 cgctgccgct ggccgccgga aacatttgtg ccgccctgtt cgatataagt attgtactga
     1741 tccggaaatt cacggataaa actgtcggcc tgtgccagct cacaggcatg aaccagctct
     1801 tcttccgtag cggattcgct gccccagcgc agattttttt tgatcgtacc ggaaaaaagc
     1861 acgtttttct gcagaacgat ggcgacctga tctctaagcg ccgcaatatc atagtcccgg
     1921 acatccacac cgcctacggt gatccttcct tccgaaatgt catacagacg ggggatcagc
     1981 tgaacaaggc tggatttggc agaacctgtg ccgccgataa taccgatcgt ctcacccgat
     2041 gcaatagaca gattgatatt gcacaaaacg ggtttatctg cctctttgga gtaagaaaag
     2101 gttacgtttt caaaagtgat cgaaccgtcc gctaccgtat ggaccggctg ctccggattt
     2161 ttcagatcgc tctcctcttc taaaatttct acgattcgct ctgccgatgc gcgggaaata
     2221 atgatcataa caaagatcat ggaaagcatc atcagactca tcaggatctg catcgcatag
     2281 gtgatcagac tggtcagttc acctgtggaa agaccgagtg ccgcatcatt gccgcaggaa
     2341 acgatcgcct tggcgccgaa ccaggagagc agaagcatgc tcatataaag gcagaactgc
     2401 atcagaggca tgttaaaggc cagtcttttt tccgcctttg agaaattttc aaaaatgacc
     2461 tgtgaaattt tttcgaattt cttttcttca aaggcttcac ggttataaga ttttacgacc
     2521 cggatgccat gaagattttc ctgcacaaca ttgttgagtt tgtcgtaagt tttgaaaaca
     2581 aatacaaaaa tctgatgtac atgcgtcatg atcagaccga gcccaagggc aagtatcgga
     2641 atgacggcaa aaaagatcag agacagtttg gcatcgatgc gaaacgaaaa gatcagggca
     2701 aagatgatca tcatcggtgc tcttaccgca agccgtacca tcattcgaat attcagaatg
     2761 catttcaaat gat
//
LOCUS       DAOSYE010000246         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27686_length_2765_cov_3.513284, whole genome shotgun sequence.
ACCESSION   DAOSYE010000246 DAOSYE010000000
VERSION     DAOSYE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27686_length_2765_cov_3.513284"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..939
                     /locus_tag="PLU43_05235"
     CDS             142..939
                     /locus_tag="PLU43_05235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOO27846.1"
                     /translation="MKYLILNGSPHRGNTWKLTEYVKSCIEEADHSSEFTEIHLRELS
                     LPFCTGCSHCFRNGEEHCPHAGQMQQVLQEFDKADGIIVSTATFNMRETGLLKNLFDH
                     MCYLLHRPRYFTKKALVITTVGGMGKAGTLKSVSGMLQGIGCNRCYRIGENSVSWNSY
                     EPLERTKKKVCRITNRFTKDVLSGRMHFPKMIELIPYNLFRGMNRDYGQEKEYATEDG
                     IYWMRPERKIGAYDRRIPLTPLHRLFGGLFFLCGIVGGRLVTVTYRK"
     regulatory      1050..1161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1299..2012
                     /locus_tag="PLU43_05240"
     CDS             1299..2012
                     /locus_tag="PLU43_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOO27847.1"
                     /translation="MEKTEIAMELKNNAYPGRGIIIGKSANGKYTAAAYFIMGRSVNS
                     RNRVFVEDGEGIRTKAFDPSKLTDPSLIIYAPVRMLGNKMIVTNGDQTDTIYDGMDHQ
                     LTFEQSLRCREFEPDGPNYTPRISGIVHMEEGSCNYALSVIKSSDGNPASCCRFTFAY
                     ENPINGEGHFIHTYQCDGDPLPSFAGEPKRIGIPDEIDEFAALLWKNLNEDNKVSLFV
                     RYIDLETLQYETRIMNKNQ"
     gene            2048..>2765
                     /locus_tag="PLU43_05245"
     CDS             2048..>2765
                     /locus_tag="PLU43_05245"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOO27848.1"
                     /translation="MKELELKYGCNPNQKPARIFCGNGGELPLQVLNGRPGYINFLDA
                     LNGWQLVKELKEATNYPSAASFKHVSPAGAAIGRPLDAVMKQIYFVDDLGELSPLACA
                     YARARGADRMSSYGDFIALSHVCDVSAAKVIKREFSDGIIAPGYEPEALAILKEKKKG
                     AYTIIQIDEKYVPEPIEHKDVFGITFEQGRNEAKINEALLENIVTKNKTIPEEARHDL
                     LISLITLKYTQSNSVCFVKDG"
BASE COUNT          839 a          519 c          732 g          675 t
ORIGIN      
        1 cgttcttcct tctgatacag atcaaggccc atttttgcgt agggatattc taaagcagga
       61 aatgggcctt gatctgtatc agaaggaaga acgggatgct tatattgatc agatcattgc
      121 agtcattatg ggagaaaaat tatgaagtat ttgattttaa atggaagtcc tcatagggga
      181 aacacgtgga agctgacgga atatgtgaag tcctgtattg aagaagccga ccacagcagt
      241 gagtttacgg aaattcattt acgtgaacta tcattgccgt tttgtacagg atgcagtcat
      301 tgcttcagga atggagaaga gcactgtccg catgccggtc agatgcagca ggtactgcag
      361 gagttcgaca aagcggacgg gattattgtt tcgacagcga cttttaatat gcgagagacc
      421 ggtcttttga aaaatttatt tgatcatatg tgttatctgc tgcacaggcc ccgctatttt
      481 acaaaaaaag cacttgtcat aactacggtc ggcggcatgg gaaaagcggg cacgttaaaa
      541 agtgtgtcgg gcatgctgca gggaatcggg tgtaaccgat gctatcgtat tggagaaaat
      601 tctgtaagct ggaatagtta tgagcctttg gaaaggacaa aaaagaaagt atgcagaatt
      661 acgaacagat ttacaaaaga cgtgctgtct ggcagaatgc atttccctaa aatgatcgag
      721 ctgattcctt ataatctctt caggggaatg aacagggatt atgggcagga aaaagaatat
      781 gcaaccgagg atggaatcta ctggatgaga ccggagagga aaatcggcgc ttatgacaga
      841 agaattccat tgacaccgct ccaccgtctg tttggcggac ttttctttct atgcgggata
      901 gttggcggac ggctggtcac agtcacttac aggaaataga aaactggttt tagttgtgga
      961 tggtgtatgg gtaaaacaat aacagatcct tacaggttgg cctggaagat aaattaatcc
     1021 ttgcatcctt tcttttaaaa tggtattatg caactgtata cgactgacgg aaagcaatgc
     1081 gggtaacaga ctgcctgtgt tgtaagtgga gtttaccaca tggagtatga cgaataacag
     1141 ccggccgtct gggcaagtgt ctccgattca aggagacgta tgcccggacg gctgtttttg
     1201 tgcataaaaa taatttttgg ccgtaatgga aacgggtgcg gtacggtgtg ccggtaaaat
     1261 aagaatagaa agatgtgttg tgtgcgaaag gagcagatat ggaaaaaacg gaaattgcca
     1321 tggaactgaa aaataatgct tatccaggac gtgggatcat aatcgggaaa tcggcaaatg
     1381 ggaaatatac cgcagccgca tattttatta tgggaagaag cgtcaactcg agaaaccgcg
     1441 tgtttgttga agacggagaa gggattcgca caaaggcgtt tgatccttcc aaactgacag
     1501 acccttcctt gattatttat gcgccggtcc gtatgcttgg aaacaagatg atcgtaacga
     1561 acggggacca gacagatacg atctatgacg ggatggatca tcagctgaca ttcgaacagt
     1621 ctttgcggtg cagggagttt gaaccggacg gaccgaatta tacaccgcgt atttcaggaa
     1681 tcgtacatat ggaagaagga agctgtaatt atgcgctgtc tgtcataaag agcagcgacg
     1741 gcaatccggc atcctgctgt cgttttacat ttgcatatga aaatccgata aacggcgaag
     1801 gacattttat tcatacatac cagtgtgacg gcgatccgct gccaagcttt gcgggagaac
     1861 cgaaacggat cgggatcccg gatgagatcg atgagtttgc ggcgctgctt tggaaaaatc
     1921 tgaacgagga caataaagtt tctttgttcg tgcgttatat cgatctggaa acgttgcaat
     1981 atgaaacaag gatcatgaac aaaaatcaat aaacacaggg aatgacagag aaaacggagg
     2041 aactgaaatg aaagaattag aattaaaata cggatgcaat ccaaaccaga agccggccag
     2101 gatcttctgc ggaaacgggg gagagcttcc gcttcaggta ttgaacggca gaccgggcta
     2161 tatcaatttt ctggatgcgc tgaacggctg gcagcttgta aaagaactga aggaggcgac
     2221 gaattacccg tctgcggcat cttttaagca tgtatctccg gcaggagcag cgatcggacg
     2281 tccacttgat gccgtaatga aacagatcta ttttgtagac gatttagggg agctttctcc
     2341 gctggcctgc gcatatgcaa gagccagggg cgcagacaga atgtcttcct atggagattt
     2401 cattgcattg tcacacgtgt gcgacgtatc ggcagccaag gtcatcaagc gtgaattttc
     2461 agatgggatc atcgcaccag gatatgaacc ggaagcgctt gccattttga aggaaaagaa
     2521 aaagggtgcc tatacgatta ttcagatcga tgagaagtat gtgccggaac cgatcgaaca
     2581 taaggacgta ttcggcatta cgtttgaaca gggccgtaat gaagcaaaga tcaatgaggc
     2641 acttcttgaa aatatcgtaa caaagaacaa aacgataccg gaggaagcca gacatgatct
     2701 gctgatctcg ctgatcacac tgaaatatac gcagtccaat tcggtctgct ttgtaaaaga
     2761 cggac
//
LOCUS       DAOSYE010000247         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27694_length_2765_cov_2.554244, whole genome shotgun sequence.
ACCESSION   DAOSYE010000247 DAOSYE010000000
VERSION     DAOSYE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27694_length_2765_cov_2.554244"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLU43_05250"
     CDS             <1..1227
                     /locus_tag="PLU43_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO27849.1"
                     /translation="ELKNTMHACLMTAAAAAVVIIGTWGSMQYVLAQETSYSLNLGAA
                     KSVAMDTEAGNTQTSLSADNTQGGQTSVSDNTTDSTTDKNGTVVEIKVSQIEGNTETD
                     TDKQIAGILYSKLVMANVEEAVNVRAEASESAELVGEFYKECAGEILEEGSGWTKIKS
                     GDLTGWVKDDYLLFGTQAQELAESVVTKTATSQAESLRVRKEPGEDAGIYGLLAKGDK
                     IEALEELGDWVSVQYTDGTVGYVSSQYVTISDELGTGETIEAINTREEAEKKAEAKAK
                     AAASTRTTETTLTNNGAITGDVNDVLLLAALIQCEAGNECYEGQVSVGTVVMNRLRTG
                     KYGSSIYSVIYANGQFSPASSGQVAQVYVQGPKATCIAAAQEAMSGVSYVGTATKFRN
                     IRSGYSGIVIGNHVFW"
     gene            1426..1860
                     /locus_tag="PLU43_05255"
     CDS             1426..1860
                     /locus_tag="PLU43_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO27850.1"
                     /translation="MDALVWLGILIVMLVIEIVTLGLTTIWFAGGALIAFVLAMFGAN
                     LWVQIPVFFVVSLVLLFVTRPIAVKYFNKDRVKTNAESLIGLDAIVISGIDNLQGVGQ
                     VTVAGQEWSAKASDDSVKYPVGSVVKIIDIKGVKVIVKEKEN"
     gene            1862..>2765
                     /locus_tag="PLU43_05260"
     CDS             1862..>2765
                     /locus_tag="PLU43_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOO27851.1"
                     /translation="MGQILVALVLILVLVILASCIKIVPQAHAFILERLGGYQSTWSV
                     GLHMKIPFIDKVARKVVLKEQVVDFAPQPVITKDNVTMRIDTVVFYQITDPKLFTYGV
                     NNPIMAIENLTATTLRNIIGDLELDQTLTSRETINTKMRASLDIATDPWGIKVNRVEL
                     KNIIPPPEIQNAMERQMKAERERREAVTRAEGEKRANILVAEGKKASKILEAEAEKEA
                     AILKAEAQKEKMIREAEGEAEAILKVQQAAADGIRFIKEAGADEAVLRIKSLEAFEKA
                     ADGQATKLIIPSDIQGMAGLAGSLK"
BASE COUNT          829 a          501 c          800 g          635 t
ORIGIN      
        1 gaattgaaaa acacaatgca tgcttgtctg atgacagctg ctgcagctgc agttgttatc
       61 atcggaacat gggggagtat gcagtatgtt ttggcacagg agacaagcta ttccttaaat
      121 ctgggcgcgg caaaatcagt ggctatggat acggaagcag ggaatacgca gacttcacta
      181 agtgcggaca atacacaggg tggtcagact tctgtcagtg ataacacgac ggattctact
      241 acggataaga atggaactgt agtagagatc aaggtcagtc agatagaagg caatacggag
      301 acagatacgg acaagcagat cgcagggatc ttatacagca agcttgttat ggcaaatgtt
      361 gaagaagcag taaatgtcag ggcagaagct tctgagagcg cagaacttgt cggggaattt
      421 tataaagaat gcgcaggcga aatcctggaa gagggttcag gatggacaaa aattaaatca
      481 ggtgatttga caggctgggt aaaggatgat tatcttcttt tcggaacgca ggcacaggag
      541 cttgccgaat cagtagtaac aaaaacagcg acttcgcagg ctgagagtct gcgcgtcaga
      601 aaagagccgg gagaagatgc cggcatatat gggcttttgg caaaaggcga taagatcgaa
      661 gcacttgagg aactgggcga ctgggtttcc gtccagtata cggatggtac ggtcggttat
      721 gtttccagtc agtatgtaac gatctcggat gagcttggta cgggtgaaac gatcgaagcg
      781 atcaatacgc gcgaagaggc tgagaagaaa gctgaagcaa aagcaaaagc tgcggcatcc
      841 acgagaacga ccgaaacgac actgacgaat aatggtgcta ttacgggaga tgtcaatgat
      901 gtcctgttac ttgcggccct gattcagtgt gaagccggaa atgaatgtta tgaaggccag
      961 gtttccgtag gtacggtagt catgaaccgt ctgcgtacag gcaaatacgg aagcagtatc
     1021 tattctgtga tctatgcgaa cggacagttc agtccggcgt cgagcggtca ggttgctcag
     1081 gtatatgtac aggggccgaa agcgacgtgc attgcagcag cacaggaagc aatgagcggc
     1141 gtgtcctatg taggtacagc cacgaagttc agaaatatca gatcaggata ttcgggtatc
     1201 gtaatcggga atcatgtgtt ctggtagacg acagcagttc cttattatgg tatgacagta
     1261 ataaaaagag acggcatgaa atcttgcgcc gtctctcttt ctatcacaga aatgtgattg
     1321 acgggcgatc gaaaaatttc cccgttcttt gttgtaaaaa aacgcaaatt atagtaaact
     1381 gttatgtatc caggtagtga aaagaaaggg gaagtggcta tggaaatgga tgcgttagta
     1441 tggcttggga tattgatcgt catgctggtc atagagattg tcacgctggg gctgacgacg
     1501 atctggtttg caggaggggc attgatcgcg tttgttttgg cgatgtttgg cgcaaatctg
     1561 tgggtgcaga ttccggtgtt ctttgttgta tcccttgtct tgctgtttgt aacacggccg
     1621 attgccgtca aatattttaa taaggacaga gtaaagacaa atgccgaaag cctgatcggt
     1681 ctggatgcga tcgtaataag cgggatcgat aatctgcagg gagtcggaca ggtgacggtc
     1741 gcagggcagg agtggtcggc aaaggcaagt gacgacagtg taaaataccc tgttggaagt
     1801 gttgtaaaaa taatcgatat aaaaggcgta aaagtgatcg taaaagaaaa ggagaattga
     1861 catgggacaa attttagttg cactcgtatt gatattagtt ctggttatat tggcttcctg
     1921 tattaagatc gtaccgcagg cacatgcatt tatacttgaa agacttggag gatatcagag
     1981 tacgtggtcc gttggacttc atatgaagat cccgtttata gacaaggttg caagaaaggt
     2041 cgttttaaag gagcaggtcg tggattttgc accgcagccg gttattacga aggataatgt
     2101 tacaatgcgg atcgatacgg tcgtatttta tcagatcacg gatccgaagc tgtttacata
     2161 tggggtaaat aatccgatca tggcgatcga gaatctgacc gccacaacgc tgcgaaatat
     2221 tatcggggat ctggagcttg accagacgct gacatccaga gagacgatca atacaaagat
     2281 gagagcttct cttgatattg cgaccgaccc atgggggatc aaggtcaaca gagtggagct
     2341 gaagaatatc attccgcctc cggaaattca gaatgcaatg gaaagacaga tgaaagctga
     2401 gcgtgaaaga cgtgaagcgg tcacaagggc ggaaggtgaa aaaagagcca atattcttgt
     2461 ggcagaaggt aaaaaagcat ccaagatcct ggaagctgag gctgaaaagg aagcagcaat
     2521 tttgaaagct gaagcccaga aagaaaaaat gatcagagaa gcagaaggtg aagcagaggc
     2581 gatcctgaaa gtacagcagg cggcggcaga cggtatccgc tttatcaaag aggcaggcgc
     2641 agatgaagcc gtgcttcgga tcaagagcct ggaggcgttt gaaaaggctg cggacggaca
     2701 ggcgacaaag ctgatcattc cttccgatat tcagggaatg gccggcctgg caggctctct
     2761 gaagg
//
LOCUS       DAOSYE010000248         2761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27755_length_2761_cov_2.633038, whole genome shotgun sequence.
ACCESSION   DAOSYE010000248 DAOSYE010000000
VERSION     DAOSYE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27755_length_2761_cov_2.633038"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..992)
                     /locus_tag="PLU43_05265"
     CDS             complement(78..992)
                     /locus_tag="PLU43_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOO27852.1"
                     /translation="MKIDRLIGILSVLLQKDKVTAPYLAERFEVSKRTISRDIEDLCR
                     AGIPIVTRQGVNGGISVMDNYKIDRTLLTTTEMQEILAGLRGLDSISGTNQYGQLMEK
                     LSAGASDFMTANQSVLIDLSLWDKGSLAPKIEMIRTGIEHNHFISFDYFSPSGESKRK
                     IEPYYLIFRWSSWYVWGWCTKRQEFRLFKLNRMDHLCISENTYEKRKVQMPDLETEKL
                     FPGGIKIEAVFCQECKWRLIEEYGPACFTERPDRKLLFHREYTNKNHLMNWLLSFGDQ
                     VELKNPGDIRMELKEIIENMRRCYESDN"
     gene            complement(1055..1924)
                     /locus_tag="PLU43_05270"
     CDS             complement(1055..1924)
                     /locus_tag="PLU43_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO27853.1"
                     /translation="MHFVTAKGILSANNGMNIYRGCTHGCIYCDSRSRCYNFTHDFED
                     IEVKQNAPELLESRLRSKRKKCMIGTGAMCDPYMQCEETLRLTRKCLEIIDKYEFGLA
                     IQTKSGRILRDLDLLKSIHEKSKCVVEMTLTTYDEALCKILEPNVSTTKERFEVLQIL
                     QENKIPTVVWLSPVLPYINDTKENIEGLLNYCIRAGVYGIITFNMGLTLREGNREYFY
                     HALDRSFPGMRETYHKKFGYSYEIISDHNAELMKLFHSTCRKHGIVSNAAEIFSYLHQ
                     FPEDTAYGQLQLF"
     gene            complement(1947..2582)
                     /locus_tag="PLU43_05275"
     CDS             complement(1947..2582)
                     /locus_tag="PLU43_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOO27854.1"
                     /translation="MAFDFKKEFKEYYLPKDRPEILEIPLMNFIAVRGKGNPGDKDGE
                     YAKAVGILYAIAYTLKMSYKGDYEIKGFFEYVVPPLEGFWWQEGIKGVDYAHKDTFHW
                     ISVIRLPDFVSEKDFIWAKQEAGRKKKMDCSAAEFLTIEEGLCVQALHTGSYDNEPET
                     VSKMNLFLSEQGYEEDLSDQRLHHEIYLSDPRKTKPEKCRTVIRHPVRMGK"
BASE COUNT          737 a          697 c          440 g          887 t
ORIGIN      
        1 tgtaaaagcg taccatcacc gccatatcat ccaccataag tccaaatccg tccaagcgca
       61 tcatctctgt ctccttttta attgtcagac tcataacatc ttcgcatatt ttctatgatc
      121 tctttcagtt ccattctgat atccccgggg ttctttaatt ctacttgatc tccaaaagac
      181 agcagccagt tcatcaaatg atttttgttt gtatactccc tgtgaaataa caattttcta
      241 tcaggacgct ccgtaaagca tgccggccca tactcctcta tcagtctcca tttacactcc
      301 tggcaaaaca cagcttctat tttgattccg ccgggaaaca gcttttctgt ttccagatca
      361 ggcatctgca cttttctctt ctcataagtg ttttcgctga tgcacaaatg atccatacga
      421 tttaatttaa acagacgaaa ctcctgtctt tttgtacacc acccccatac ataccagctc
      481 gaccagcgaa aaatcaaata atacggctcg atttttcgtt ttgattctcc tgaaggtgaa
      541 aaataatcaa aagaaataaa atgattatgt tcgattccgg ttcggatcat ctcaatttta
      601 ggagccagtg agcctttatc ccacaatgac agatcaatca ggacagattg atttgccgtc
      661 ataaaatcag aagctcctgc cgataatttc tccattaact gtccatactg atttgttccg
      721 ctgatgctgt caaggccgcg cagtcctgcc aatatttcct gcatttccgt tgtagtaaga
      781 agcgttctgt cgatcttgta attgtccata acagatatgc cgccgtttac cccctgcctt
      841 gttacgatcg gaatcccagc tctgcacaga tcctcaatat cacggcttat cgttcttttg
      901 gacacttcaa aacgttctgc cagataaggc gccgtcacct tatctttctg taataaaaca
      961 gataaaattc cgatcagccg gtcaattttc atagtatgct ttccttttat aaattcattt
     1021 tacactaaca aacatgtcat ctgtatgtct tgttttaaaa caactgcagc tgtccgtatg
     1081 cagtatcttc aggaaactgg tgcagatatg aaaatatctc tgccgcattg ctcacgatcc
     1141 catgcttcct gcacgtactg tgaaacagct tcatcagttc cgcattatga tcacttatta
     1201 tctcatacga atacccgaat tttttgtgat aagtctccct cataccggga aaggaacgat
     1261 ccagggcgtg ataaaaatat tccctgtttc cttcccgcag cgttaagccc atattaaacg
     1321 taatgatccc gtacacaccg gccctgatac aatagttaag gagcccttcg atattttctt
     1381 ttgtgtcatt gatatacggc aaaaccggac ttagccatac aactgtcgga atcttatttt
     1441 cctgcaggat ctgcagaact tcaaaccgtt cctttgtagt tgaaacattc ggctccaaaa
     1501 ttttgcacag tgcttcatca taggtcgtca gcgtcatttc cacaacacat tttgactttt
     1561 catgaatgct ttttagcaga tccagatccc gcagtattcg tcctgatttt gtctgtatcg
     1621 caagtccgaa ttcgtattta tcgataattt ccagacattt tctcgtcaac cgcagcgttt
     1681 cctcgcattg catatatgga tcacacatag cgcccgtacc gatcatgcat ttttttcttt
     1741 tggaacgcag tctgctttcc aaaagctccg gcgcattctg tttgacttcg atatcctcaa
     1801 aatcatgtgt aaaattataa cacctgctgc ggctgtcaca ataaatgcac ccgtgcgtac
     1861 atcctctgta aatattcata ccattgtttg cagataaaat tcccttagcc gttacaaaat
     1921 gcatagattc ctcccggata cctgtcttat ttccccatac gaaccgggtg tctgatcact
     1981 gttctgcatt tttcaggttt tgttttccgc ggatcggaca aatagatctc atggtgcagc
     2041 ctctgatcgg aaaggtcttc ctcatagccc tgttctgata aaaacagatt cattttggag
     2101 actgtttccg gttcgttatc ataggaaccc gtatgcaatg cctgcacaca caaaccttcc
     2161 tctatcgtaa gaaactcagc agcagaacaa tccattttct tctttctgcc cgcttcctgc
     2221 tttgcccata taaaatcctt ttcagacaca aaatccggaa gccggataac cgatatccaa
     2281 tgaaatgtat ctttatgcgc atagtctacg cctttgattc cttcctgcca ccaaaagcct
     2341 tccagcgggg gaacgacata ttcaaagaat cctttgattt cataatcccc tttataactc
     2401 attttcagtg tataggcaat tgcatagaga ataccgacag cctttgcata ttcaccgtct
     2461 ttatctcccg gatttccctt tccgcgtacc gcaataaaat tcatcagcgg aatttccagt
     2521 atttccggcc tgtcttttgg cagataatac tctttaaatt cttttttaaa atcaaacgcc
     2581 atgtgatacc agtctccttt tcttcttttt atcgatcata tcatatcaaa tacggcatct
     2641 gcatgtcata ttataggacc ggtataaatt tctggcttta tcaggcatct gtatcgaaca
     2701 gctttctata ttgatcaaaa aaatcaaata ttttcacaaa agcttcatca tcaatcttta
     2761 t
//
LOCUS       DAOSYE010000249         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27922_length_2749_cov_2.740906, whole genome shotgun sequence.
ACCESSION   DAOSYE010000249 DAOSYE010000000
VERSION     DAOSYE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27922_length_2749_cov_2.740906"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLU43_05280"
     CDS             <1..816
                     /locus_tag="PLU43_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO27855.1"
                     /translation="DKDMKDMFTEANGYDASFAYSLKNDEQITAAQKFIQDGVDYLLL
                     SAADTAGWDSVLQDAQDAGIRVILFDRTIDADESLYEASIVSDMAKEGQTAVDWLASQ
                     GLDEYNIIHIQGVMGSSAQKGRTGALDEACEADNWTLVTQQTAEWNAEKAQQIVQSVI
                     DSGESFNVIYAENDDMAKGAVAALDKANISHGVGKDVIVMGFDCNQWALEELLAQNWN
                     YDGQCNPFQASYIDEIIKTLESGGTLSEKTIIMDEKGFDATTITQDDVDTYGI"
     gene            948..2441
                     /locus_tag="PLU43_05285"
     CDS             948..2441
                     /locus_tag="PLU43_05285"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO27856.1"
                     /translation="MKNIHKSFPGVHALQGVDFTLCEGEIHALMGENGAGKSTLIKVL
                     TGVYGKDEGQIFLKGKEKEVVIRSPQDAQRMGISTVYQEITLCPNLSVAENLYIGRTN
                     SSIQNWKKMNEGADKILKQLDIPTKAKQQLASCSLAVQQMVAIARAVDMDCKVLILDE
                     PTSSLDEQEVEKLFGLMRDLKSRGVGIIFVTHFLDQVYEVCDRITVLRDGKLVGEYEI
                     KDLPRVQLVSKMLGKELDDLSDIRDDSLVYAKKDTAVPVFEVEKLESNAGIKPFNFYI
                     KKGEVNGFTGLLGSGRSECVRAIFGADRVISGSVKKNGKTIKITKPIDAMRNGIAYLP
                     EDRKRDGIVGDLSVRENIVLALQVLKGFFKPFTKAEANEFADEYIKLLGIKTASADIP
                     VKSLSGGNQQKVILARWLLANPEYLILDEPTRGIDVGTKIDIQKLVLKLASEGMSITF
                     ISSETDEMLRTCSRLIVMRDRKVVGELSGNELTQTMIMSTIAGGDKK"
     gene            2441..>2749
                     /locus_tag="PLU43_05290"
     CDS             2441..>2749
                     /locus_tag="PLU43_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27857.1"
                     /translation="MKNDTQLVNISAFFKRLFKRQIFIPIAALLLLALFNLISDPSFY
                     KITLGYNSSGAPVLSGYLMTILDYGSELAILAIGMTLVTSASGGQDISVGAAIAISGS
                     V"
BASE COUNT          807 a          520 c          726 g          696 t
ORIGIN      
        1 gataaggata tgaaagacat gtttacggaa gcaaacggat acgatgcatc atttgcatac
       61 agcctgaaga atgatgagca gatcacagca gctcagaagt tcatccagga tggtgttgat
      121 tatctgcttc tctccgcagc agatacagca ggctgggatt cggttctgca ggacgcacag
      181 gacgcaggca tcagggtaat cctgtttgac cgtacaatcg acgctgatga aagtctttat
      241 gaagcatcta tcgtttccga tatggctaaa gaaggccaga cagcagttga ctggttagca
      301 agccagggac ttgatgagta taacatcatt cacattcagg gtgttatggg atcttcagca
      361 cagaagggac gtacaggcgc tcttgacgaa gcatgtgagg cagataactg gacgcttgtt
      421 acacagcaga cagcagagtg gaacgctgaa aaagcacagc agatcgttca gtcagttatt
      481 gattccggtg aatctttcaa cgtaatctat gcagagaacg acgatatggc aaaaggcgct
      541 gttgcagctc ttgacaaagc aaacatctct catggtgttg gcaaagatgt catcgttatg
      601 ggctttgact gcaaccagtg ggcattggaa gaactgcttg cacagaactg gaattatgac
      661 ggacagtgta atcctttcca ggcttcctat atagatgaaa tcatcaagac gcttgagagc
      721 ggcggaacac tttccgaaaa gacgatcatc atggatgaga agggctttga tgctacgacg
      781 atcacccagg acgatgttga tacttacggt atttaagtaa gaccggtttg tgactgataa
      841 aagcgcggcg cagcgtaaat gaagaatcgt acgcttgctc cgcgcttatt gtctaaaata
      901 ctttgaaagg agtgaataca ctggtgaaag ataaatccgt tttggaaatg aaaaatatac
      961 ataagagctt tcccggggta cacgctctgc agggcgtgga ttttacgttg tgcgagggcg
     1021 agatacatgc tctgatgggt gaaaatggcg ccggaaaatc gactttgata aaagttctga
     1081 ccggagttta cgggaaagat gagggtcaga ttttcctcaa aggaaaagaa aaggaagttg
     1141 tcatacgttc accacaggat gcgcagagaa tggggatcag taccgtatat caggagatta
     1201 cgctttgccc gaatctttcg gttgcagaga atttgtatat aggacggaca aattcttcaa
     1261 ttcagaattg gaaaaaaatg aatgaggggg cggataaaat actgaaacag cttgatattc
     1321 cgacaaaagc gaaacagcag ctggcaagct gttctcttgc agtgcagcag atggttgcga
     1381 ttgcacgtgc ggtggatatg gactgtaaag tattgattct ggatgagccg acctcttctc
     1441 tggacgaaca ggaggtcgaa aagctgtttg gcctgatgcg cgatctgaag agcagaggtg
     1501 ttggaattat ttttgtcaca catttcttgg atcaggtata tgaggtatgt gacaggatta
     1561 cggttttacg tgatggaaag cttgtaggag agtatgagat caaggatctt ccgcgtgttc
     1621 agcttgtttc taaaatgctt ggcaaggagc tggatgatct gtcggatatc cgtgacgaca
     1681 gtttggtata tgcaaagaaa gatactgccg taccggtatt tgaagtagaa aaactggaaa
     1741 gcaatgcagg tatcaagcca tttaatttct acatcaaaaa aggggaggta aatggcttta
     1801 caggtctgct tggctccgga agaagcgaat gtgtacgtgc gattttcggt gctgacaggg
     1861 tgatcagcgg aagtgtgaaa aagaatggaa aaacaattaa gatcacgaag ccgatcgatg
     1921 ccatgagaaa cggtatcgcc tatcttcctg aagaccgtaa gagagatggt atcgtaggag
     1981 acctttccgt acgcgaaaat attgtacttg cactgcaggt actgaaggga ttttttaaac
     2041 cgtttacaaa agccgaggca aacgagtttg cggatgaata tatcaagctt ttggggatca
     2101 aaacggcatc tgcggacata ccggttaaat cactttccgg aggaaaccag caaaaggtga
     2161 tccttgcccg ctggctgctt gccaatcccg aatacctgat actggatgaa ccgacacgtg
     2221 gtattgatgt cggtacaaag atcgatatac agaaattagt attgaaactt gcttcagaag
     2281 gtatgagcat cacgttcatt tcctctgaaa cggatgaaat gctccgtaca tgttcacgtc
     2341 tgattgttat gcgagaccgt aaagtggttg gagagctttc aggcaacgag ctgactcaaa
     2401 ctatgattat gagtacgatt gccggaggtg acaaaaaata atgaaaaatg atactcaatt
     2461 agtaaatata tctgcatttt ttaagagact gtttaagcgg cagatattta ttccaatagc
     2521 ggctctgctg ctgctggccc tgttcaatct gatttcggat ccttcctttt ataagatcac
     2581 actgggctat aacagctcgg gagcgcctgt tctgtcaggc tatctgatga cgatcctgga
     2641 ttacggatcg gaacttgcga ttcttgcaat cggtatgaca ctcgtgacat ctgccagcgg
     2701 cggacaggac atcagtgtcg gcgcagcgat cgcaatcagc ggaagtgtg
//
LOCUS       DAOSYE010000250         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_27934_length_2748_cov_3.835499, whole genome shotgun sequence.
ACCESSION   DAOSYE010000250 DAOSYE010000000
VERSION     DAOSYE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27934_length_2748_cov_3.835499"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLU43_05295"
     CDS             <1..1177
                     /locus_tag="PLU43_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27858.1"
                     /translation="FEKVPYINREGNAGIRLVFLPMQSYLFCFMKKEQPTGYKKEPIQ
                     IQNVEMKKEWNVKEPFCNALTLDYCDFRINEEEWNLHTAVIKVQDALLARREPCKVTL
                     RFLFEAELDLYKNKECCLVLEDAKKYALCVNGYDISTKETGWWKDQAFRTVSIKPYLK
                     NGSNEILLSVQFEQPQKVYDVLFGEQVYETEKNKLTYGIEIENIYLIGDFGVYSRSSF
                     VKAERNALLTKGPFVIRDMPEQFTGLALTEQGCLFLADKIVLSQQIRVKKEPDKRQML
                     TFGGQKAPLMKLYVNQAFVKNFMWEPYEADITDELSDGDNEIVLELFASNRNLLGPHH
                     HIDGECYHVGPESFTGKWSWVERETEADATDISDRNKNYWTDTYSFIEFGMEQIWTD"
     gene            1165..2595
                     /locus_tag="PLU43_05300"
     CDS             1165..2595
                     /locus_tag="PLU43_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOO27859.1"
                     /translation="MDRLNYMETVENYKKIESRDYGEVCDSLKKSLLANAAKLIVLDD
                     DPTGIQAVHDVCVYTDWSEKSIVSGFRRPETMFYILTNSRAMSELETYALHKEIAARV
                     VKISKELQMDFMLISRADSTLRGHYPLETMTLRQMLEAAGDRQVAGEILVPYFYAGGR
                     FTIQNIHYVKSGEQLIPAGETEFAQDKTFGYNSSNLCDYIEEKTKGAYSAKDVTCISL
                     ESLRRCEMEKVTEQLLQVHDFGKVIVNALSKRDLIVFCAALYRAVEKGKFFLFRTAAD
                     FVKVFGGITDKELLSKNELITKSNGRGGIVIIGSHTEKTTSQLQELKKNRELCFMEFQ
                     SDLVVDNKLDEEVKRVVSWSEECILDGRTAVIYTNRKLLTFADDTKEAALQRSVRISA
                     AVSRLVGDLSVEPAFVVAKGGITSSDIGVKALSVKEAYVLGQIQPGIPVWKTDDASRF
                     PGIPYVIFPGNVGEIDTLKKVVEILL"
     gene            2604..>2748
                     /locus_tag="PLU43_05305"
     CDS             2604..>2748
                     /locus_tag="PLU43_05305"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOO27860.1"
                     /translation="MISVAPVDAADKVNDPEKIAEDVYACFQLGASMVHLHCRDKNGR
                     LTSD"
BASE COUNT          846 a          486 c          727 g          689 t
ORIGIN      
        1 atttgaaaaa gttccttaca taaacagaga gggaaatgca ggcataaggc ttgtgttcct
       61 tccgatgcag tcttacctgt tttgttttat gaagaaggag cagccgaccg gatataaaaa
      121 agagccgatt caaattcaaa atgtagagat gaaaaaggaa tggaatgtaa aagaaccgtt
      181 ttgcaatgca ctgacactgg actattgtga ttttcgcata aacgaagagg aatggaacct
      241 gcataccgct gttataaaag tgcaggatgc gctgcttgcc agaagagaac cgtgtaaagt
      301 aacgctgcgt tttttatttg aagcggaact ggatctttat aaaaacaaag aatgctgtct
      361 tgttcttgag gatgcaaaaa agtatgcgct ttgcgtaaac ggatacgata tttcgacgaa
      421 ggagaccggc tggtggaaag atcaggcatt caggaccgtt tccataaaac cgtatttgaa
      481 aaacggaagc aatgaaattt tattgtccgt gcagtttgag cagccgcaga aagtgtacga
      541 tgtcctgttt ggagagcagg tttatgagac agaaaagaat aagcttactt acggaatcga
      601 gatcgaaaat atctatttaa tcggagattt tggtgtttat tccagatctt cgttcgtaaa
      661 agcagagaga aacgcgctgc tgacaaaagg tccgtttgtt atacgggata tgccggaaca
      721 gtttacgggt cttgcactga cagagcaggg ctgcctgttt ttggccgata aaatagttct
      781 ttcacagcag atcagggtga aaaaagagcc ggacaaaagg cagatgctga cgtttggcgg
      841 tcagaaggct cctcttatga agctttatgt taatcaggcg tttgttaaaa attttatgtg
      901 ggagccgtat gaagcggata ttacagatga attgtccgac ggtgacaatg agatcgttct
      961 tgaattattt gcatcaaaca gaaatctttt aggtccgcat catcatatcg acggagaatg
     1021 ttatcatgtc ggaccggaga gttttaccgg aaaatggagc tgggtcgaga gagagaccga
     1081 agccgatgca accgatattt ccgacagaaa taaaaattac tggacagata cgtatagttt
     1141 tattgagttt ggaatggagc agatatggac agactgaatt acatggaaac agtggaaaac
     1201 tataaaaaaa tagagagcag agattacggg gaagtctgcg acagcctgaa aaagagcctt
     1261 ctggcaaatg cggcaaaact gatcgtgctc gatgatgacc cgacagggat ccaggcagtg
     1321 cacgatgtct gtgtctatac ggactggtct gaaaaaagca tagtatccgg atttcgccgt
     1381 ccggaaacga tgttctatat actgacgaat tccagagcta tgtcagagct ggaaacgtac
     1441 gcgcttcata aggaaatagc ggctcgtgtt gtgaaaatat caaaagaact gcagatggac
     1501 ttcatgctga tcagccgtgc ggattctacg ctgcgaggac attatccgct tgagaccatg
     1561 acgcttaggc agatgctgga agcagcaggg gatcgtcagg tggcaggaga gatccttgtt
     1621 ccgtattttt atgcgggagg acgttttacg attcaaaaca ttcattacgt aaaatccggt
     1681 gaacagctga taccggccgg tgagacggaa tttgcacagg ataagacatt cggatacaac
     1741 agttccaatc tgtgtgatta tattgaagaa aagacaaagg gagcatattc tgcaaaggat
     1801 gtgacctgta tttcgcttga aagtcttcga agatgcgaga tggaaaaggt cacggaacag
     1861 ctgcttcagg tgcatgattt tggaaaagta attgtaaacg cactttctaa aagggatctg
     1921 atcgtatttt gcgctgcgct ttatcgggct gttgaaaaag ggaaattttt tctgtttcga
     1981 acggcagcag attttgtaaa ggtgtttggc gggattacgg ataaagagct gctgtcaaaa
     2041 aatgaattga ttacaaaatc aaacggccgc ggcggaatcg tcatcatcgg ctctcatacg
     2101 gaaaaaacaa caagccagct tcaggaatta aaaaagaaca gggaactgtg ttttatggag
     2161 tttcagtcag accttgtagt tgataataaa ttggacgaag aagtaaaaag ggtggtttcc
     2221 tggagcgaag aatgtatttt ggacggacgc acggcagtga tctatacgaa caggaagctg
     2281 ctgacttttg ccgatgatac aaaggaagcc gcactgcaaa gatcagtcag aatatctgcc
     2341 gctgtcagca gactggtcgg cgatctgtcc gtggagccgg catttgttgt ggcaaagggc
     2401 gggatcacgt ccagtgatat cggcgtaaag gcactttccg tgaaggaagc gtatgtactc
     2461 ggacagatcc agccggggat cccggtctgg aaaacagatg atgccagcag gtttccagga
     2521 ataccatatg ttatttttcc cggaaatgtg ggagaaatcg atacattaaa aaaggtggtt
     2581 gaaatattat tatgaaacag atcatgattt ccgtggcacc ggtcgatgcg gctgataaag
     2641 tgaatgatcc ggaaaaaata gcagaggacg tatatgcgtg ttttcagctt ggcgcatcca
     2701 tggtgcattt gcactgcagg gacaaaaacg gacggctgac atctgatc
//
LOCUS       DAOSYE010000251         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28134_length_2736_cov_2.707572, whole genome shotgun sequence.
ACCESSION   DAOSYE010000251 DAOSYE010000000
VERSION     DAOSYE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28134_length_2736_cov_2.707572"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..430
                     /locus_tag="PLU43_05310"
     CDS             8..430
                     /locus_tag="PLU43_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOO27861.1"
                     /translation="MIQLIVGEKGKGKTKHLLDKVNNAVKTVTGNIVYLDKNSKHMYE
                     LKNKIRLINVSEYPVTSTGEFVGFICGIVSQDHDLEQMYLDSFLDVSYIRDHKDIESV
                     INKLEKISEQFHVDFVISMSVKDSELSEAMKSMIIVSL"
     gene            504..1904
                     /locus_tag="PLU43_05315"
     CDS             504..1904
                     /locus_tag="PLU43_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOO27862.1"
                     /translation="MAKKRKRPKKVTKYRKPININLGLIIFGVLFIYIIVIVVSYFQS
                     VHITPYEVTTGSLAVNNTYTGVALREETVIPSDYSGYINYYAREGEKISNGSMVYTVD
                     TTGKLSEMVNEDTGDGSSLTDDDLSELKTEISDFSTSFSPEDFSTAYDFKFDIEGTVL
                     KLANYKVLSSIDTLADSNLSDSINFGYAPQSGVLVYSTDGYEDVTADTLTPEMMDPAN
                     YAKNQFHSNDLIAEGDSAYKLVTSENWSIIIEVDEERALELEQESYIDVRFLKNNYTS
                     WASISILRQNDAIYAKLDFNNSMITFATDRMIEIELLANSEEGLKIPNSAIVERAFYL
                     IPTEYLTQGGNTGADGFIRETYLEDGTVSTEFVEATIYNSTDTEYYVDESVFNIGDYI
                     IMPDSGEKYPISKQATLIGVYNINKGYADFKQITILYQNEEYSIVKSNTEYGLSVYDH
                     IVLNGDTVNENDFIYN"
     gene            1947..2639
                     /locus_tag="PLU43_05320"
     CDS             1947..2639
                     /locus_tag="PLU43_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOO27863.1"
                     /translation="MLKDNLNNVEAVISQTCQKAGRDRADVRLIAVSKTKPIEMLQEI
                     YDCGIRDFGENKVQELASKFEQLPKDIRWHLIGHLQTNKVKYIIDKVFLIHSVDSYKL
                     AMEISKQAVKHQVNAGILIEVNVSEEESKFGVKCGEVTELLEQISVLPNIFVKGLMTV
                     APYVDDPEENRTVFSKLKQLSVDITRKNIDNVTMEYLSMGMSGDYPVAIEEGAVYVRV
                     GTSIFGERDYNK"
BASE COUNT          879 a          474 c          596 g          787 t
ORIGIN      
        1 gattacaatg atacaattaa tcgttggcga aaaggggaaa gggaagacaa agcatttact
       61 tgataaagta aacaatgctg ttaagacggt tacaggtaat attgtatatt tggacaaaaa
      121 ctcaaaacat atgtatgaat taaaaaataa aatccgcctg attaatgttt cggagtatcc
      181 cgtgacatca acaggcgaat ttgttggttt tatctgtgga atcgtgtccc aggatcacga
      241 cctggaacag atgtatctgg atagttttct ggatgtttcc tatatcagag accataagga
      301 tattgaatct gtaattaata aacttgaaaa gatcagtgaa cagtttcatg ttgattttgt
      361 tatcagtatg tcggttaagg attcggagtt atctgaagcg atgaaatcca tgattattgt
      421 atctctttaa tcaaatagga atattataga tcatcaatga aaagcctcgg ctgtccgggg
      481 cttttcatgt gtaggaaagt attatggcaa aaaagagaaa aagaccgaaa aaagtaacca
      541 aatacagaaa acctatcaat attaatcttg gattgattat ttttggtgtg ctctttatct
      601 atattattgt cattgttgtt tcatattttc aaagtgtgca tattacgccg tatgaagtca
      661 cgaccggctc gcttgccgtc aataatacct ataccggcgt tgccctgcgg gaagaaacgg
      721 ttattccgtc cgattattcc ggatacatca actattatgc cagagaaggg gaaaagatct
      781 ccaatggttc tatggtatat accgtagaca caacaggtaa gctttctgaa atggtcaatg
      841 aagatacagg agacggcagc tcccttaccg atgatgatct ttccgaacta aaaactgaaa
      901 tctcagattt cagcacaagc ttcagtccag aagatttttc cacagcctat gatttcaaat
      961 ttgatattga aggtactgtc ttaaagcttg cgaattacaa agtactgtca agtatcgata
     1021 ccctggcgga ttcaaacctg tcagactcta ttaatttcgg ctatgcaccg cagtccggtg
     1081 ttcttgttta ttcaacagac ggctatgaag atgtcacggc agacactttg acaccggaga
     1141 tgatggatcc ggccaattat gcaaaaaacc agtttcacag caatgatctg atcgctgaag
     1201 gagattctgc ctacaaattg gtcacaagcg aaaactggtc cattataatt gaagttgatg
     1261 aagaaagagc acttgaactg gaacaggaat cctatatcga tgtccgcttc ctgaaaaata
     1321 attatacatc atgggcttcc atttcaattt tgcggcaaaa tgatgcgatc tatgccaaac
     1381 tggatttcaa taattccatg atcacatttg ctacagacag aatgattgag atcgaactgc
     1441 ttgccaattc cgaagaaggt ctgaaaattc cgaactctgc aattgttgag cgtgcttttt
     1501 acctgattcc aaccgaatat ctgacacagg gcggcaatac cggtgcagat ggttttatcc
     1561 gtgaaacata tctggaggac ggtactgttt ccacggaatt tgttgaagct actatttaca
     1621 attcaaccga tacggagtat tatgtggatg aatcggtatt caatatcgga gattatatta
     1681 ttatgcctga cagcggtgag aaatatccga tcagcaagca ggcaacattg atcggtgttt
     1741 ataatattaa taaaggatat gctgatttca agcagatcac gatcctgtat cagaacgaag
     1801 aatattctat cgtgaaatcc aatacggaat atggactcag cgtatatgac catattgttt
     1861 taaatggcga tactgtaaat gaaaatgatt ttatttacaa ctagaccgca gccggtatca
     1921 atagtattta ggattagagg gattttatgc taaaagataa tttgaacaac gtggaggctg
     1981 tgatctccca gacatgtcag aaggccggaa gagaccgtgc cgatgttaga ctgatagctg
     2041 tcagtaaaac aaaacccata gagatgctgc aggaaatcta tgactgcggg atccgtgatt
     2101 tcggcgaaaa taaagtacag gagcttgcct ccaaatttga acaactgccg aaagatatcc
     2161 gctggcattt gatcggacat ctgcagacaa ataaagttaa atatatcatt gataaggttt
     2221 ttcttatcca cagtgtggat tcctataagc ttgccatgga aatcagcaaa caggccgtaa
     2281 agcaccaggt gaatgccggc atactgatcg aagtcaacgt ttctgaggag gaaagtaaat
     2341 ttggtgtgaa gtgcggcgaa gtgactgaat tgctggaaca aatttccgtt ttgcctaata
     2401 tttttgtcaa aggtctgatg accgtagcac cttatgttga tgatccggag gaaaatcgta
     2461 cggtttttag caaattaaaa caattatctg ttgacataac aaggaaaaac attgataatg
     2521 taactatgga gtacctatcc atgggcatgt cgggtgatta tccggttgcg atagaagaag
     2581 gggctgttta tgtccgtgtg ggaacttcca tttttggtga gcgggattac aataagtaaa
     2641 gaaagataag gagaatatta aatatggcat ttttggataa gttgttaagc tcaatgagat
     2701 ataacgacga tgacgattat gatgacgagt tctttg
//
LOCUS       DAOSYE010000252         2709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28557_length_2709_cov_3.250565, whole genome shotgun sequence.
ACCESSION   DAOSYE010000252 DAOSYE010000000
VERSION     DAOSYE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28557_length_2709_cov_3.250565"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLU43_05325"
     CDS             <1..452
                     /locus_tag="PLU43_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HOO27864.1"
                     /translation="PDMVYWGKHRHVMGRSSEWWESVKIGGGAAPIETSEGWLLFYHG
                     VSSTCNGFVYSIGGAILDIENPSIVKYRCENFLLTPEAWYEERGFVPNVVFPCATIHD
                     PKSGRIALYYGTADTYVGLAFMEFDEIMTYIKEHSVLSKSDMETGRR"
     gene            673..1851
                     /locus_tag="PLU43_05330"
     CDS             673..1851
                     /locus_tag="PLU43_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOO27865.1"
                     /translation="MLIQEVTKHLTNDIIPFWKDLRDDMFGGYCGWLGYDLQPDKKAE
                     KGCILNSRILWFFSNACLTLQDKSLLDEAAHAYAFLREHCVDKEYGGVYWSLHYDGTV
                     KDSTKHTYNQAFAIYALSAYYDASKDEEALKLAGELQQLIEKNCTDEIGYLEAFTYDF
                     RPESNEKLSENGVMAEKTMNTLLHVFEAYTEYYRVTKDAFTADRLRFMMDMIADKIYN
                     PILKRQEVFFDKYMNSILDLHSYGHDIETAWLVDRGCDILGDEEYTKKLRPITKALTE
                     KIYERAYVKHSLMNECENGVDNKMRIWWVQAEAVVGFMNGYQRAPEQTKYLQAAVDIW
                     NFIKKYVIDRREGSEWFWQVDENGKPDEEKPIVEPWKCPYHNGRMCMEIIRRKIDAAS
                     "
     gene            1838..>2709
                     /locus_tag="PLU43_05335"
     CDS             1838..>2709
                     /locus_tag="PLU43_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOO27866.1"
                     /translation="MLHPEYYVELDKYQKLIERPNRKSNIYNGIYDRWEYPVLTRSHI
                     PLTWKYDLDLETNPYFMERLGVNAVMNSGAIELNGKFYLVARIEGNDRKSFFGIAESS
                     TGVDGFRFWDYPILLEDTCPEETNAYDMRLTKHEDGYIYGVFCSESKDTSVNDLSAAV
                     AAAGIVRTKDLKTWERLDNLKTLHSPQQRNVVLHPEFVNGKYAFYTRPMDDFIDTGSG
                     GGIGFGLCDDVTHAVIDEEIITSMRKYHTITEAKNGAGCVPIKTEKGWIHIAHGVRNT
                     AAGLRYVIYAFATDL"
BASE COUNT          798 a          491 c          745 g          675 t
ORIGIN      
        1 gtccggatat ggtctattgg ggaaaacacc gccatgtgat gggcagaagc agcgaatggt
       61 gggaatctgt aaagatcgga ggcggagccg caccgatcga gacctcggaa ggctggctgc
      121 tgttttatca tggtgtcagc agcacatgca atggttttgt gtacagtatc ggcggcgcga
      181 ttctggatat cgaaaatccg tcgatcgtaa aataccgctg tgaaaatttc cttctgacgc
      241 cggaagcgtg gtatgaggaa cgcggttttg taccgaatgt tgtgtttccg tgtgcaacga
      301 tccatgatcc aaagagcgga aggatcgcat tgtattacgg tacggcagac acttacgtag
      361 gtctggcttt tatggaattt gatgagatca tgacttatat caaagagcac agcgtactgt
      421 cgaaatccga tatggaaacc ggccgcagat agccggaaat aaaacgataa aaagtactat
      481 ttgggtattg caattactca aagattagta taatattaag taataaaaga gaaaaatatt
      541 aagttaatta agcaaacaaa atttgccggc agaccggcga aacagtacag cgagtcttat
      601 cttgtcagga ctcgctgtgt ggcgctgtgg cggaggattc cggcacttca tgcggaagcg
      661 gaggagaagc tgatgttgat tcaggaagta acgaagcacc ttaccaatga tatcattcca
      721 ttttggaaag atctgcggga tgatatgttt ggcgggtact gcggatggct cggatatgat
      781 ctgcagcctg acaaaaaagc cgaaaagggg tgtattttaa acagcaggat tttatggttt
      841 ttttcaaatg cctgtctgac actgcaggat aaaagcctgc ttgatgaagc ggcacatgca
      901 tatgcgtttt tgcgggagca ttgtgttgac aaagagtatg gtggtgtata ctggtccctg
      961 cactatgacg ggacagtgaa ggacagcaca aagcatacat ataatcaggc atttgcgatc
     1021 tatgcgctgt cggcctatta cgatgcctcc aaagatgaag aggctctgaa gctggcaggg
     1081 gagctgcagc agctgatcga aaaaaactgt acggatgaga tcgggtatct ggaagctttt
     1141 acgtatgatt tcaggccgga gagcaatgaa aagctttccg aaaacggagt tatggcagag
     1201 aagacaatga atacactgct gcacgtattt gaagcatata cggaatatta ccgtgtgaca
     1261 aaggatgcat ttacggcaga ccgtctgcgg tttatgatgg atatgatcgc ggacaaaatt
     1321 tataatccca ttctgaaacg gcaggaagta tttttcgata aatatatgaa cagcatcctg
     1381 gacctgcatt cttacgggca tgatatcgag acggcctggc ttgttgacag gggctgcgat
     1441 atcttgggag atgaggaata tacgaagaag ctgcgtccga tcacaaaagc gctgacggaa
     1501 aaaatttacg aacgcgccta tgtgaagcat tccctgatga atgagtgtga aaatggtgtg
     1561 gataataaaa tgcggatctg gtgggtacag gccgaggctg ttgtcggttt tatgaacggc
     1621 tatcaaagag cgcctgaaca gacaaagtat ctgcaggcag ctgtcgatat ctggaatttt
     1681 attaaaaaat atgtgatcga caggcgggaa ggttccgaat ggttctggca ggtggatgaa
     1741 aacggaaagc ctgacgagga aaaaccgatc gtagaaccat ggaaatgccc gtatcataac
     1801 ggcagaatgt gtatggaaat cataaggagg aaaatagatg ctgcatcctg aatattatgt
     1861 agaactggac aaatatcaaa agcttattga aagaccgaac agaaaatcaa atatttataa
     1921 tggaatttat gatcgatggg aatatccggt attgacaaga agtcatattc cgcttacatg
     1981 gaaatatgat ctggatctgg agacgaatcc gtattttatg gaacggcttg gtgtaaatgc
     2041 ggtaatgaat tccggtgcga tcgagctgaa cggaaagttt tatctggtag cccggatcga
     2101 gggtaatgac agaaaatcct tttttggaat tgcggaaagc agtaccggtg tagacggatt
     2161 tcggttctgg gattatccga tcttacttga ggacacctgt ccggaggaaa cgaacgcgta
     2221 tgatatgcgt ctgacaaagc atgaagatgg ctatatttac ggggtattct gttcggaatc
     2281 aaaggatacg agcgtgaatg acctgtcggc tgccgttgcg gcggcaggga tcgtccgcac
     2341 aaaagactta aagacctggg aacgtcttga caatctgaaa acactgcatt ccccgcagca
     2401 gagaaatgtt gtgctgcatc cggaatttgt gaatggtaaa tatgcgtttt atacaagacc
     2461 gatggatgat tttatcgaca ccggttccgg aggcggtatc ggcttcggtt tgtgtgatga
     2521 tgttacgcat gcggtcattg atgaggagat cattacaagc atgcggaagt atcatacgat
     2581 cacagaggcg aaaaacggtg cgggctgtgt tccgatcaag acagaaaagg gctggatcca
     2641 tattgcgcat ggagttcgca atacggcagc aggactgcgc tatgtcattt atgcatttgc
     2701 cactgatct
//
LOCUS       DAOSYE010000253         2707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28586_length_2707_cov_3.346531, whole genome shotgun sequence.
ACCESSION   DAOSYE010000253 DAOSYE010000000
VERSION     DAOSYE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28586_length_2707_cov_3.346531"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLU43_05340"
     CDS             complement(<1..1053)
                     /locus_tag="PLU43_05340"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO27867.1"
                     /translation="MKNILEMKDIDKSFPGVHALNHCSFSLKEGEIHALVGENGAGKS
                     TLVKIMTGIYSDYEGEYSYDGQPVHFHNIREAQYAGISMVHQELNMMNDLTVAQNIFI
                     GRESKGFFCSDNEINEKTQKLIREFDIHVNAKDILKNLSVGKAQMVEIARAMSFDSTK
                     VLILDEPTAALSEAEAEDLFRKMNGLKKKGISIIFISHRLGEIRKIADRVTIMRDGAY
                     IDTLPISECSKDVMIRLMVGREILEEPKQHSNVGPDAPVVLEAKNLHSSKVKDVTFSL
                     KKGEILGFAGLVGAGRTETMRLIYGADPMKGGEIMINGAPCVIKHSKDAIGNGIGYLS
                     EDRKRYGLILGLSVTDN"
     gene            complement(1262..2560)
                     /locus_tag="PLU43_05345"
     CDS             complement(1262..2560)
                     /locus_tag="PLU43_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27868.1"
                     /translation="MKRKKKIFLAGCLCALAVSAVLAIAVINRSEKETILKFAMFSDS
                     NWGVSGDNTSKIVEQAIKEFEEEHPGVKVVYESGIRKEDYTEWLEGKILLGEEPDIFM
                     VSADVFNILASKGALLDLREFMDEDQTFREDRFYHVAIESGEFEQKQYALPYESVPML
                     MAVNITLLNELQIDLPENDWTWSDFHAICRRVTRDTDSDGKIDQFGVYNYTWKDAAYT
                     NGTLLFDKNGKENYVSHQDVINAVNYVHKINMLTDGYLVTAKDFESGTVAFSPMLLSE
                     YRMYKNYPYSIEKYNNFEWTCLTMPAGPKGDNISEVDTLLTAISSRSNYRKLAWEFMK
                     KLTCDEGIQERVARESKGVSVLKDVTENTQVYDPEDEIDRSLLSLSMQKGVIVPRFYQ
                     YDEAMRIMDEGVQEAMESEKNIKASLIALHKKIDSFLKNE"
     gene            complement(2557..>2707)
                     /locus_tag="PLU43_05350"
     CDS             complement(2557..>2707)
                     /locus_tag="PLU43_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOO27869.1"
                     /translation="SNKEIASELSLSEGTVRNYISMILNKLNLRDRTQLAIWTVQTGV
                     VSEQL"
BASE COUNT          661 a          710 c          461 g          875 t
ORIGIN      
        1 attgtcggta acggacaagc ccaatatcaa accgtatctt tttctgtcct cggataaata
       61 accgatgccg tttccgatcg cgtccttgga atgtttgatc acacaggggg ctccatttat
      121 cataatttct ccgcccttca tcggatccgc cccatagatc agtctcatgg tttctgttct
      181 gcctgctccg acaagtcctg cgaaaccaag gatctctccc tttttcaatg aaaacgtaac
      241 gtcttttact ttagaagaat gcagattctt tgcttccaaa actacaggcg catccggccc
      301 aacattgctg tgctgttttg gttcttcaag aatttccctg ccaaccatca aacggatcat
      361 aacatccttg ctgcactcgc ttatcggcag tgtatcaata taggcgccat cccgcatgat
      421 cgtaacacgg tcggcgatct tcctgatctc tcccagtctg tgggaaataa aaataatgga
      481 aatgcctttt ttctttaagc cgttcatttt ccggaacaga tcctctgctt cggcttccga
      541 caaagcagcc gtaggctcgt ccagaatcaa aaccttcgtc gaatcaaagg acatggctct
      601 tgcaatttca accatctgtg cctttccgac ggaaagattt ttcaaaatat ctttcgcatt
      661 tacgtgaata tcaaattccc tgatcagctt ctgcgttttt tcattgatct cattatccga
      721 acaaaaaaac cctttggatt ctctgccgat aaatatattc tgtgccacgg tcagatcatt
      781 catcatattc agttcctggt gtaccatgga aatgccggca tactgcgctt ctctgatatt
      841 atgaaaatga accggctgcc cgtcatagct gtattctcct tcataatcgc tgtaaatgcc
      901 ggtcattatt ttaaccagcg tggattttcc ggctccattt tctccgacca gcgcatgtat
      961 ttctccctct ttcaaagaaa aactgcagtg attcaatgcg tgaacgcccg gaaaagattt
     1021 atcaatatcc ttcatctcta aaatattttt catttcctat tctctcctcg gtttcttcct
     1081 gtcagctgta tttatcatag caattttcct gcatcgaaac atctgttaag catcacaact
     1141 tcctgttcac atgtgtcata atccgtcttt ttttcgatgt gacatttatc atttctttag
     1201 cagacggagc tttcctgcaa caaaaaacca actgcctgtt tcttctgcaa ttggttttaa
     1261 atcactcgtt ttttaaaaaa ctgtctatct ttttatgaag cgctattaaa gatgctttaa
     1321 tgtttttttc actctccatg gcttcctgca cgccttcatc cataattctc attgcttcgt
     1381 catattgata aaaccgcgga acaatgactc ccttctgcat ggacagcgac aaaagcgacc
     1441 tgtcgatttc atcttccgga tcatatacct gtgtattttc cgttacatct tttaaaaccg
     1501 acactccttt ggattccctg gcgacccgct cctgaattcc ctcatcacag gtcagctttt
     1561 tcatgaattc ccaggccagt ttccggtagt tgcttcggct gctgatcgcg gtcagcagcg
     1621 tgtccacctc cgatatatta tctcctttcg gtcctgcagg cattgtcagg caggtccatt
     1681 cgaaattatt atatttctca atactatacg gataattttt atacatccgg tattcggaaa
     1741 gcagcatcgg tgaaaaagca actgttccgg attcgaaatc tttagctgtt acaagatacc
     1801 cgtctgtcag catgtttatt ttatgtacat aattgactgc attgatcaca tcctgatgac
     1861 ttacataatt ttcttttcca tttttgtcaa acagcagcgt tccgttcgta tatgccgcgt
     1921 ccttccatgt ataattatat acaccaaact gatctatttt cccgtcgctg tccgtatctc
     1981 ttgtcactct gcggcagatc gcatgaaaat cgctccacgt ccagtcattt tcaggcagat
     2041 ctatctgcag ctcattcaga agcgttatat ttaccgccat cagcattggc acggattcat
     2101 agggcagtgc atattgtttc tgctcaaact ctccggattc tatcgcgaca tgataaaacc
     2161 gatcttcccg aaaagtttga tcttcatcca taaactcccg cagatcaagc agtgcgcctt
     2221 tagacgcaag tatattaaat acatcggccg ataccataaa gatgtccggc tcttccccaa
     2281 gcaatatttt cccttccagc cattccgtat aatcttcttt ccgtatcccg ctttcatata
     2341 cgaccttaac acccgggtgt tcttcttcaa actctttaat tgcctgctca acgatttttg
     2401 atgtattatc cccggatact ccccaattgc tgtcggaaaa catggcaaat ttcaaaatcg
     2461 tttctttttc ggatcgattt atgactgcaa ttgcaagtac tgccgatact gccagcgcac
     2521 aaaggcatcc ggccagaaaa atttttttct tccttttcat aactgttccg ataccacccc
     2581 tgtctgcacg gtccatattg ccagctgagt cctgtcgcga agattcagct tgtttaaaat
     2641 catgctgata tagtttcgta cggtcccttc cgacagcgac agctccgacg cgatctcctt
     2701 gttggac
//
LOCUS       DAOSYE010000254         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28692_length_2701_cov_3.721844, whole genome shotgun sequence.
ACCESSION   DAOSYE010000254 DAOSYE010000000
VERSION     DAOSYE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28692_length_2701_cov_3.721844"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="PLU43_05355"
     CDS             <1..1689
                     /locus_tag="PLU43_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO27870.1"
                     /translation="DVDNASFRQVVEDLTAKYGKKIAPFHLPIRENEKFVGYVNVVSE
                     TGNRWTKEGKVEECPIPDYSKANLKICREALMEAVAETSEDFMERYFGGESFSDAEIR
                     SALRANIIDGSIVPVTMGSSILCQGVYTLMDDIVKYFQGPDTRKIAGIHMKTSEIFAA
                     DYDFAKPKSVYIWKTIVDPFIGKYSLIKVCSGVIKSDDVLFNYDKDIEEKISKLYVLR
                     GNKPIEIAELHAGDIGAIAKLTGARTGDTLSTKTNPIQFAKTEISKPYTYMRYKAKNK
                     ADIDKISGALQKMMREDLTLKNVNDSENRQTLLYGMGDQHLDVVASKLLNVYKVEIEL
                     TQPKVAYRETIKKTSDVESKYKKQSGGHGQYGHVKMKFEPLDDVSKAYEFEQIVVGGA
                     VPKNYFPAVEKGLAESVQKGPLAAYPVVGVKAVLYDGSYHPVDSSEQAFKMATIQAFK
                     KGFMEAGPILLEPIASLKVTVPDAYTGDVMGDLNKRRGRVLGMIPVPGGKQTVEAEIP
                     MTGLFGYCTVLRSMTGGRGIYEYEFNRYEQAPSDVQEQEVKEREGKLNDGSVDA"
     misc_binding    1857..2075
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2113..>2701
                     /locus_tag="PLU43_05360"
     CDS             2113..>2701
                     /locus_tag="PLU43_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO27871.1"
                     /translation="MAVPYNHREVEQKWTEEWEKMPVNVDDGKRPKYYCLDMFPYPSG
                     NGLHVGHWRGYVISDVWSRYKLLNGYYIVHPMGWDAFGLPAENYAIKMGVHPAKSTAE
                     NVKNIKKQINQIAALYDWDMEVNTTDPDFYKWTQWIFVKMFREGLAYEKEMPINWCPS
                     CKTGLANEEVVDGKCERCGAEVTKKNLKQWMLQITQ"
BASE COUNT          818 a          476 c          742 g          665 t
ORIGIN      
        1 gatgtggaca atgccagttt tcggcaggtc gttgaggatc tgactgcaaa atacggaaaa
       61 aagatcgcac cgtttcactt gccgatcagg gaaaacgaaa agtttgtcgg gtatgtcaac
      121 gtagtcagtg aaaccggtaa ccgctggaca aaagaaggaa aagtagagga gtgtccgatt
      181 cccgattatt ccaaggcaaa tctgaaaata tgcagggaag ctttgatgga agcggttgcg
      241 gagacaagcg aagactttat ggagcgttat tttggcggag aatctttttc agatgcggaa
      301 atacgttctg cactgcgggc gaatattatt gatgggagta ttgtgccggt gacgatgggc
      361 tccagtattc tgtgccaggg tgtgtatacg ctgatggatg atattgtcaa atattttcag
      421 ggtccggata caagaaaaat cgcaggcatt catatgaaga caagcgagat ctttgcggcg
      481 gattatgatt ttgccaaacc aaagtcggtc tatatatgga agacgatcgt tgatccgttt
      541 attggaaagt attctctgat aaaggtctgt tccggcgtta tcaaatcaga tgatgtactg
      601 tttaattatg ataaggatat tgaagagaag atttcaaaat tatatgtatt acgcgggaat
      661 aaaccaatag agatcgcgga acttcatgcc ggggatattg gtgccattgc caaactgaca
      721 ggtgcccgta cgggagatac gttgtccaca aagaccaatc cgattcaatt tgccaaaacg
      781 gaaatcagca agccatatac gtatatgaga tataaagcaa agaataaggc agatattgat
      841 aaaatttcgg gtgccctgca gaagatgatg cgtgaagatc tgaccctcaa aaatgtaaat
      901 gattccgaaa acagacagac actattatat gggatgggtg accagcatct tgacgttgtg
      961 gcaagcaaac tgctgaatgt ctataaagtg gaaattgagc tgactcagcc aaaggttgca
     1021 tacagggaaa cgatcaaaaa gacttccgat gtggaatcca aatataaaaa acagtccggc
     1081 ggacatgggc agtatggaca tgtaaaaatg aaatttgagc cgcttgacga tgtgagcaag
     1141 gcctatgaat ttgaacagat cgttgtaggc ggagccgtac cgaagaatta cttcccggca
     1201 gttgaaaaag gacttgcgga atcggtgcag aaaggtccgc tggcagctta tccggtcgtt
     1261 ggtgtgaagg cggtcttata cgatggatct tatcatccgg tggattcgtc tgaacaggca
     1321 tttaaaatgg ctacgatcca ggcatttaaa aaaggattca tggaggcagg accgatcctg
     1381 cttgagccga tcgcatcact taaagtgacc gtaccggatg cctatacggg tgatgttatg
     1441 ggtgacttga ataaacgccg cgggcgtgtg cttggcatga taccggttcc gggaggaaag
     1501 cagaccgtag aggcggagat tccgatgacg ggtctgttcg gctattgtac ggtgcttcgt
     1561 tccatgaccg gaggccgcgg tatttatgag tatgagttta accgttatga gcaggctccg
     1621 tccgatgtac aggagcagga agtgaaagag agggaaggca agctgaatga cggatcagta
     1681 gatgcatagc tcccctgttt tacataaaac aagacatgat atttagatgg caaaagatgc
     1741 aggctgaaaa agttctgtat cttttgctgt gatttgctgt tttgttcaat aattctgaag
     1801 aaatcgtgta tgagtgcctt gacaggcata gtaatcatgg ttaaaatata gcatagtaaa
     1861 aaacaatgat gaggaatagt agaagtggag tgttttcaga gagctgccgg caggtgcgag
     1921 gcagtacaca tagctttgaa ctcacctcgg agttcctctg ctgaacggtt ttcgtttagt
     1981 aggcaggggc gggtgtcccg ttacagatga tgagtgcata cggttgatac gtatgaaata
     2041 gagtggtacc acggaaaacc cctttcgtct ctatgagaac gaaggggtat tttttttcag
     2101 gaggagacaa aaatggcagt accttataat cacagagaag tggaacaaaa atggacagag
     2161 gaatgggaaa aaatgcccgt taatgtagat gacgggaaac ggccgaagta ttattgcctg
     2221 gatatgtttc cgtatccgtc aggaaacggt ctgcacgtcg gacattggag aggatatgtg
     2281 atctctgacg tatggagcag atataaactg ctgaacggct attatatcgt ccatccgatg
     2341 ggctgggatg catttgggct ccctgctgag aattatgcga tcaagatggg ggttcatccg
     2401 gcaaagtcca ctgcggaaaa tgtaaaaaat attaagaaac agatcaatca gatcgcggcg
     2461 ctttatgact gggatatgga agtcaatacg acagatccgg acttttataa atggacacag
     2521 tggatttttg tgaaaatgtt cagggaagga cttgcctacg aaaaagaaat gccgatcaac
     2581 tggtgtccgt cctgtaagac agggcttgcc aatgaagaag tcgtggacgg aaaatgcgaa
     2641 cgctgcgggg ctgaagtaac gaaaaaaaat ctgaagcagt ggatgctgca gatcacgcag
     2701 t
//
LOCUS       DAOSYE010000255         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28711_length_2700_cov_3.392817, whole genome shotgun sequence.
ACCESSION   DAOSYE010000255 DAOSYE010000000
VERSION     DAOSYE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28711_length_2700_cov_3.392817"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLU43_05365"
     CDS             complement(<1..203)
                     /locus_tag="PLU43_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27872.1"
                     /translation="MNDHKYKTKNLYFSPRLLDKLEDIWNHPLTVVEAPMGYGKTTAV
                     KEYLKKTNAPVLWLRIYRDSAEA"
     gene            386..1033
                     /locus_tag="PLU43_05370"
     CDS             386..1033
                     /locus_tag="PLU43_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO27873.1"
                     /translation="MDRKKEFAGLIENAESTKQKLFYTGLDLFSQKGFANVGIRELCY
                     SVHVKESAFYNHYAGKEKLLEAILNYFDETSSQVIMSDDEIAEFVKKGDVRYFFTENM
                     KRFSMLTSNILYHTALQIVLTESFLHPLAGEMAKKNLYHLRKDYTEKVLCGLMECKAV
                     KKCDAELVTAEYYYALKGILDEYLLLETWESSMSEIKERLKGHIEFFAALLEYKN"
     gene            1050..1322
                     /locus_tag="PLU43_05375"
     CDS             1050..1322
                     /locus_tag="PLU43_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27874.1"
                     /translation="MRTTGFLAVILAAVSAGFGIWTAVAGKPYHAGLFAGSEIMTVVI
                     FGIAFAALLITGAIMGAGKSVTAALRAGHTVAAAVSVIAGSVLSVF"
     gene            1334..1504
                     /locus_tag="PLU43_05380"
     CDS             1334..1504
                     /locus_tag="PLU43_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27875.1"
                     /translation="MLRKVLSNVYFIHFLVLLGLFALWGMIESGNRYLVLFAVLSLAG
                     AVCGAFIRVIDS"
     gene            complement(1524..2423)
                     /locus_tag="PLU43_05385"
     CDS             complement(1524..2423)
                     /locus_tag="PLU43_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOO27876.1"
                     /translation="MKWKRILGWSGVSITVVFSSLWAYWGAFENFHEGCYASFLENIF
                     MFFFQYLLFAIIFTVLALISLKWKKAGLLLHLAVSIFCAWFFAKAAFSVLWLLIIIPF
                     AVLAVCYHFGEPYPLSWAYRLILFIPLTIVLAISIPQGIKVANRIDDGDLGVRTIEDN
                     GVSLAFAPRGPGWPDEGVTWEEAQTLCSYLSEDGLTIMDSKQNIWRLPTVDEAVRSMM
                     LHGENAGGIWYAQDEKAVYEKTPDKESPLWDVHSKIIYYWTADTSVQDPKQAYIIVYN
                     GGVYDKRKTDRLGYLSFRAVKDL"
     gene            complement(2484..>2700)
                     /locus_tag="PLU43_05390"
     CDS             complement(2484..>2700)
                     /locus_tag="PLU43_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO27877.1"
                     /translation="DTSLKQTRFNIELLYIQDADKVADMGNAFSITCPSCGAPVTDIG
                     QKTCAYCGCAIAEINIHAWALNRYYET"
BASE COUNT          758 a          509 c          657 g          776 t
ORIGIN      
        1 aaggcttctg ctgaatcacg gtatattctc agccagagga caggggcatt cgtttttttc
       61 agatattcct ttacggcagt tgtctttcca tagcccatcg gtgcttccac gactgtcagc
      121 ggatgattcc agatatcctc cagtttatca aggagacgcg gagaaaaata caggtttttt
      181 gttttatatt tatgatcgtt caaaattgaa taccgccttt tttaaaaggt gagcaggcac
      241 ggccggacag gctttgcact gtttttatta tagcatacga attttatcgc ggtcaatttg
      301 tgtagatttg gtttaaaaga aacttgacag aaagcagtat gtgagatatt atattaacga
      361 acgttaatta ataaaaggaa acaatatgga cagaaaaaaa gaatttgccg gactgataga
      421 aaatgcagag tctaccaaac aaaagctgtt ttatacagga cttgatctgt tttcgcaaaa
      481 aggttttgca aatgtcggaa tcagggagct ttgttattcc gtgcatgtaa aagaaagcgc
      541 gttttataat cattatgcag gaaaggaaaa gcttttagaa gcgatcttaa attattttga
      601 tgagacgagc agccaggtta ttatgtcgga tgatgaaatt gcagaatttg tgaaaaaagg
      661 tgacgtgcgg tattttttca cggaaaatat gaagcgtttt agtatgctta catcgaatat
      721 tttatatcat acggcactgc agatcgtgct gaccgaatca tttttacatc cgctggcagg
      781 tgaaatggca aaaaagaatc tttatcatct tagaaaggat tacacagaaa aggtgctttg
      841 tggtcttatg gagtgcaagg ctgtgaaaaa atgcgatgca gagcttgtaa cggcagagta
      901 ctattatgca cttaaaggta ttttggatga gtatctgtta ctggaaacat gggagagcag
      961 catgtcggaa ataaaagagc ggctgaaagg ccacattgaa ttttttgctg cgctgctgga
     1021 atacaaaaat tgagagggag gatatatttg tgagaacaac gggattttta gcagtgattc
     1081 tggcggcagt gtctgccggt tttggaatat ggacagctgt tgctggaaaa ccctatcatg
     1141 ccggtctgtt tgcaggctct gaaataatga cggtggttat tttcggaatt gcgtttgcag
     1201 cgctgcttat cacaggtgcg atcatggggg cggggaaatc cgtaactgct gctttgcgtg
     1261 ccggacacac tgtcgctgca gcagtatctg ttatagcagg gagtgtattg tctgtgtttt
     1321 aaggaggtaa ggaatgctga gaaaagtatt gagcaatgtt tattttattc attttctcgt
     1381 tctgcttggt ttatttgcac tatggggaat gatcgaatca ggcaaccggt atttggtact
     1441 gtttgcggtc ctgtctttgg caggggctgt gtgcggagcg tttatcaggg tgattgattc
     1501 ttaataaatg ccatgacaaa aatttataga tcttttacgg ctctgaagga aagatagccg
     1561 aggcggtcgg tttttctttt gtcatatacg ccgccgttgt atacgataat atatgcctgt
     1621 tttggatcct ggacagaggt atcggcagtc cagtaataaa tgatctttga atgaacgtcc
     1681 cacagcggag attccttgtc gggcgttttt tcataaactg ctttttcgtc ctgcgcatac
     1741 cagatgccgc cggcattttc tccgtgaagc atcatggaac gaacggcttc gtcaacagtc
     1801 ggcagtctcc atatattttg tttactgtcc atgattgtca gaccgtcctc tgataaatag
     1861 ctgcagagcg tctgcgcttc ttcccatgta acaccttcgt ccggccatcc ggggcccctt
     1921 ggtgcaaaag cgagggatac gccattgtct tcgatcgttc tgactccaag atctccgtcg
     1981 tcgattctgt ttgcgacctt gattccctgc ggaatggaga tcgcgaggac gatagtcagc
     2041 ggaatgaata aaataagccg gtaagcccat gaaaggggat acggctctcc aaagtgatag
     2101 cagacggcta aaacagcaaa cggtatgatg ataagcagcc ataatacact gaaagcagct
     2161 tttgcaaaaa accatgcaca gaaaatactc acagccagat ggaggagaag gccggctttt
     2221 ttccatttca gactgatcag tgccagtact gtaaagatga tcgcaaataa taaatattga
     2281 aagaaaaaca tgaaaatatt ttccaggaag gatgcatagc agccttcgtg aaagttttca
     2341 aaagcgcccc aataagccca aagactggaa aaaacaacag tgatagatac gccggaccag
     2401 ccaagtatcc ttttccattt catgatccac ctccgtatga aatacgatgg tttccctttc
     2461 gttcagtatc tgtaagatcg gattcaggtt tcataatatc tgttcagtgc ccatgcatga
     2521 atattgattt ccgcaatggc acagccgcaa taggcacatg tcttttgccc gatatcggtg
     2581 acaggggcgc cgcagctcgg acaggtaatg gaaaaagcat ttcccatatc ggcgactttg
     2641 tccgcatcct gtatgtaaag cagttcgata ttgaatcggg tctgtttaag ggaggtatcg
//
LOCUS       DAOSYE010000256         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28780_length_2696_cov_3.542598, whole genome shotgun sequence.
ACCESSION   DAOSYE010000256 DAOSYE010000000
VERSION     DAOSYE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28780_length_2696_cov_3.542598"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PLU43_05395"
     CDS             complement(<1..1265)
                     /locus_tag="PLU43_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO27878.1"
                     /translation="MKLKKVLAVCLSMAMTLGLLSGCGVSTDTGAVTEDTSAAAEEAA
                     PADDTAEADTAAAADTASGNVQNITWMFWDDLDATEDLISLGYKDVIDRFNADYEGVY
                     HCDVITTNLEEYYTKLNALVASNQTPDVFIVSPGPNLTTYVEPGVAADLTDYLKTDDP
                     DWYASFNGGVFDKQTYDGKIYAVPTNLAAACVFYNTEMFDAAGVEVPTTWTEMIDACQ
                     KLQDAGYTPVTISAGTAWCLSMLAGYLCDREGADLDGVNAGTASWLDQPFVDAANKML
                     ELSQYFQPTAAGDTNDIATANFYDGEAAMLIQGSWAIAQINGANPDFEDKCGVFQFPG
                     IEGGNDPNRMIAKTDSLAMSSSTEYPEASLALIKYFTDDTAQKYTAEVGGKIPVTAVD
                     VDYDKAPKQLKYVMDIFGKTTGTFGFYNES"
     gene            1720..2454
                     /locus_tag="PLU43_05400"
     CDS             1720..2454
                     /locus_tag="PLU43_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO27879.1"
                     /translation="MPLENGYHIECNDILFHLRVGDIIIIAPGVLHRLYAEHGRRIIF
                     QADMNLLNPFTEFESFFSFMQPAAVITPEDFPTIHDDSVQLMTEIKDEYFSSAPLREA
                     AICAKLLQILVMVSRLYTTLPNKFTDIKPNKQQEYIEKFITVCDYINQHCTEDLSLDE
                     IANMAGFSKYHFSRLFKEFTNVSFYKYLNGRRIAYAEKLLLDPEINVMEAAVRSGFNS
                     ISAFMRMFKIIKNCTPTEFRNLYHKA"
BASE COUNT          752 a          602 c          486 g          856 t
ORIGIN      
        1 gattcattgt aaaatccaaa tgtacctgtt gtctttccga aaatatccat tacatatttc
       61 aactgcttcg gtgctttgtc ataatctaca tcaactgctg ttacaggaat ctttccgccg
      121 acttctgctg tatacttctg agctgtatca tccgtaaaat atttgataag tgcaagagac
      181 gcttccggat attccgttga agaactcatg gcaagactgt ctgtctttgc aatcattctg
      241 ttaggatcgt ttccgccttc aatacccggg aactggaata cgccgcactt gtcttcaaaa
      301 tcaggatttg caccgttgat ctgtgcaatt gcccaggaac cctggatcag cattgcagct
      361 tcgccatcat aaaagtttgc cgttgctata tcattcgtat cgcctgctgc agtcggctgg
      421 aaatactgtg ataattcaag catcttgttt gccgcatcta caaacggctg atcaagccag
      481 cttgctgtac ctgcattaac accatcaaga tcagcacctt ctctgtcaca gagatatcct
      541 gccaacatag acagacacca tgcagtacct gcgctgatcg taacaggtgt gtatcctgcg
      601 tcctgcaatt tctgacatgc atcaatcatc tctgtccatg ttgtaggaac ctcaacgcct
      661 gccgcatcaa acatttcggt attataaaat acgcatgctg ccgcaagatt agtcggtacc
      721 gcataaatct ttccgtcata agtctgcttg tcaaatacac cgccgttaaa gcttgcatac
      781 cagtccggat catctgtctt cagatagtct gtcagatccg ccgcaacacc cggttctaca
      841 taagtagtaa ggttaggacc cggacttacg ataaatacat ccggtgtctg gtttgatgct
      901 accaaagcat tcagctttgt atagtattct tccagattcg ttgtaataac atcgcaatga
      961 taaacgcctt cgtaatctgc attaaatctg tcaatgacat ctttatagcc aagtgagatc
     1021 agatcttccg ttgcatccag atcatcccag aacatccatg tgatattctg tacattaccg
     1081 cttgcagtgt ctgcggctgc cgctgtgtct gcttctgccg tatcatctgc cggagctgct
     1141 tcctctgcag ctgcagaggt gtcttctgtt acggcgcctg tatctgtcga tactccacag
     1201 ccggaaagaa gaccaagtgt cattgccata ctgagacata ctgctaaaac ctttttcaac
     1261 ttcataattt gtcctcctaa tcaaaaattt gaaatatgtt tttggtatat tcatcataaa
     1321 tgaaaccgta tgctttcttc tatacaataa ttgctttttg attattaatt attgcgattg
     1381 atttttatat tttttgtttc gtaaaactat attttgtcta attattctaa ttaattttaa
     1441 ttgattttgt atatattgta tattgtttaa ttttcttttt acaattatta ttattttagt
     1501 gtatattatt tttaatttta tttgttttct ttgtttataa aatagcacga attattatca
     1561 tttttctcaa ttcaatgctt aaaaggagct tttatgatca aaatactaaa cggtattcac
     1621 gaaacagtca gctttgaaaa cgactctgcc ctgatgctct atgacaatac cgattatgaa
     1681 gaatatccaa cacactgaca cacgcctgtg gaaatcataa tgccgctgga aaacggttat
     1741 catatcgaat gcaatgatat cctcttccat cttagggtcg gcgacattat tattatcgcg
     1801 ccgggtgttc ttcacagact atacgcagaa cacggacgac ggatcatctt tcaggccgat
     1861 atgaacctgc tgaacccgtt cacggaattt gaatcattct tttctttcat gcagccggct
     1921 gcagttatca ctccggaaga ttttccgacg atccacgacg attccgttca gctgatgaca
     1981 gagatcaaag acgaatattt cagcagcgcg cctttgcggg aagctgccat atgcgcaaag
     2041 cttttacaga tcctcgttat ggtaagccgt ctgtatacga ctcttcccaa caaatttacg
     2101 gacatcaagc caaacaaaca gcaggaatat attgaaaaat tcattacagt atgcgactat
     2161 atcaaccagc actgtacgga agacctgtct cttgatgaga tcgcaaatat ggcaggcttt
     2221 tcaaagtatc atttcagcag gctttttaaa gaattcacaa atgtctcatt ctataaatat
     2281 ctaaacggca gaagaatcgc ctatgccgaa aaactgctgc ttgatcctga aatcaatgtc
     2341 atggaggcgg ccgttcgttc cggttttaac agtatttccg cattcatgcg catgtttaag
     2401 atcatcaaaa actgtacacc tacggagttt cgaaatctgt atcataaagc ataaatttca
     2461 tgcatatttg ccgcatgtgt attttgacgt atttccgaat atatggtata tttatgaata
     2521 aaatactgcc agtccagaat cggaggatac catgttcggt caaatattat tgcagattct
     2581 attaattttc tgctgctccc tttttacatg tgcggagatc acgatcattt cttttaatga
     2641 tgcgaaactg gataagcttt cagcagacgg caacagacag gcacagaaac tgaaaa
//
LOCUS       DAOSYE010000257         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28862_length_2692_cov_3.609405, whole genome shotgun sequence.
ACCESSION   DAOSYE010000257 DAOSYE010000000
VERSION     DAOSYE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28862_length_2692_cov_3.609405"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1313
                     /locus_tag="PLU43_05405"
     CDS             36..1313
                     /locus_tag="PLU43_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO27880.1"
                     /translation="MLWKMSVPSILGVLAYNLYNIVDTIFISKGAGIDAIGGVAVSFP
                     LFLLLSAVSSTLGNGAASVISRALGNKDPEKAEKAAANTFALFYLIAILITVTGLLFL
                     KPLLYSMGVTEELMPYAQRYTRIILFGAVTSTGFSSLIRAEGNSRYAMYIWVIPLSVN
                     ILLDPIFIFGFRMGVTGAAAATVAAQIISAGMSIYYFYLSKKSSLRLKPEHFIPDKEL
                     LREIILIGIPSFLQMTGYSFSIILVNQILKKYGGNLYISTYGIVSKINTFLLIPVMGL
                     VQGLQPVIGYNKGADRPDRIKEALKKASAAAGGYGLAAYGVIMLFSSQLLQIFTSDSV
                     VIGRGAVILQIVNIGIVCTALQYIQSVYFQAIGKKLLSLLVVLSNQVICFIPALLFLS
                     GRYGLTGVWYAFPVSSVLALLISTVVLFRTFRE"
     gene            1424..1852
                     /locus_tag="PLU43_05410"
     CDS             1424..1852
                     /locus_tag="PLU43_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOO27881.1"
                     /translation="MKQLTRSQEHYLKAVLKCSLGSESARICEVAEDLHVKKSSVCTA
                     MKKLEHMGLIERNEHQIFLTEKGWEHVNLGIDRLEIIKTFLVSVLDVEEKAAQLDACA
                     MEHVISNKTLCSMCRQISKAGASHICPNDCRVKISRRNDG"
     gene            1952..>2692
                     /locus_tag="PLU43_05415"
     CDS             1952..>2692
                     /locus_tag="PLU43_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27882.1"
                     /translation="MENPHIIRANKVLFIVVSIVTVFWAAGLFSQLVLSDESPLISII
                     PLIMLGMIYIGDLVVHFRKKLYRVFLPYVVITFLIFYAIVLLTARSNLTYPFLIPVMF
                     AFIPYQNKKVSTISGIYLIIINAIKIGTIISKTEDMNTVNESIMIEFTISVLCGLVLI
                     LGTRLIIRFFEESSVEVQKAALHNKEMAEKVVNSAKFVLEKVEHSGQALNSITENTSA
                     VSETIEDISKSALITSENIEQQTEMTGSI"
BASE COUNT          756 a          494 c          638 g          804 t
ORIGIN      
        1 atcagacatt tttagaagaa gcgccagtcg gaaagctgtt atggaaaatg tccgttccat
       61 cgatccttgg ggtgctggcc tataatttat ataatattgt cgatactatt tttatttcaa
      121 aaggagcagg gatcgatgcg atcggcggag tggccgtttc ctttccgctg tttcttctgt
      181 tgtccgctgt ttcctcgact ctcggaaacg gtgcggcttc cgtgatatcc agagcacttg
      241 gaaataaaga tcctgaaaaa gcagaaaaag cagctgcgaa tacattcgcg ctgttttatt
      301 taattgcaat tctgattacc gtaaccggtc ttcttttttt gaaaccgctt ttatatagta
      361 tgggtgtgac agaggaactg atgccgtatg cgcagaggta tacgaggatc atcctgtttg
      421 gggccgttac aagtaccggg ttttcaagcc tgatcagggc agaaggaaac agcagatacg
      481 cgatgtatat ctgggtgatc cccctgtctg taaatatact ccttgatccg atatttattt
      541 tcggttttcg gatgggagtc acaggggctg ccgcagcaac ggtcgcagct cagattatct
      601 ccgctgggat gagtatttat tatttttacc tgtcaaagaa aagctcactg aggctgaagc
      661 cggagcattt tattcccgat aaagagctgc tgcgtgagat catcctgatc ggaattccgt
      721 cttttttaca gatgacaggc tatagctttt caattattct cgtcaaccag atcttaaaaa
      781 aatacggcgg gaatttatac atcagtacct atgggatcgt cagtaagatc aacacgtttc
      841 ttttaattcc tgttatgggg cttgtgcagg gtctgcagcc ggttatcgga tataataaag
      901 gcgcagacag gccggacaga ataaaggagg cattaaaaaa ggcttccgct gccgcgggcg
      961 gatatggact ggcggcttac ggcgtcatta tgctgttttc aagtcagctg ctccagatct
     1021 ttacttcgga ttctgttgtg atcggcaggg gagccgtcat tttgcagatc gtgaatatag
     1081 gcattgtctg tacggctctg caatatatcc agtctgttta ttttcaggcg atcggaaaga
     1141 aactgctgtc attgcttgtc gttctcagca atcaggtcat ttgctttatt ccggcacttc
     1201 tgtttctgtc aggccgatat ggactgaccg gagtctggta tgcgtttccg gtttcttcgg
     1261 tgctcgcatt gcttatttca accgttgttc tgttcagaac ctttagagag taattttgtc
     1321 aatgaattgc ttttatttag ttgaaagtta gttgcaacta atctataatt aatgtgaaac
     1381 gaactaacga aaaatcaggc aaaggaggtg tggtttatct attatgaaac agttgacacg
     1441 atcacaggaa cattacctga aggctgtgct gaagtgttct ttgggcagcg aaagtgcgcg
     1501 catatgcgag gtggcagaag atcttcatgt caaaaaatcg agtgtgtgta ctgcaatgaa
     1561 aaaattggag catatggggc tgatcgaaag aaatgaacat cagatctttc ttactgaaaa
     1621 aggctgggaa catgtgaatt tgggaataga cagattggag atcatcaaaa cgtttcttgt
     1681 cagcgttctt gatgttgaag aaaaggctgc acaactggac gcctgtgcca tggaacatgt
     1741 tatcagcaat aaaacacttt gttcaatgtg ccggcagatt tctaaagccg gtgcttccca
     1801 tatatgtccg aatgattgca gggtaaaaat aagcagaagg aatgacggct gaaagcaggt
     1861 attgccagaa agaggatttt cccgtataat ggaaaatata gatcatgatg cataatgcag
     1921 gtgttatgag taaaccaggg agggaataaa gatggaaaat ccgcatatta ttcgggcaaa
     1981 taaagtgttg tttatagtgg tatctattgt aactgtattt tgggcagccg gattgttttc
     2041 acagctggta ctttcggatg aatcaccgtt gatcagtatt attccgctga tcatgctggg
     2101 gatgatttat attggcgacc ttgttgttca tttcagaaaa aaactgtacc gtgtttttct
     2161 gccgtatgta gtaatcacgt ttctgatttt ctatgccatt gtattgctga cggccagatc
     2221 caatctgaca tatccgtttc tgatacctgt catgtttgca tttatacctt atcagaataa
     2281 aaaggtatct acgatatctg gaatttatct gatcataatc aatgccataa aaataggaac
     2341 cattatttcg aaaaccgagg atatgaatac ggtaaatgaa tcgatcatga tcgaatttac
     2401 catttctgtg ttatgcggac tcgttcttat tttaggaaca agactgatta tccgattttt
     2461 tgaagaatcg tccgttgaag tgcagaaagc cgctcttcac aataaggaaa tggcagagaa
     2521 ggtcgtaaat tctgcaaaat ttgtattgga aaaggtagaa cattccggtc aggcattaaa
     2581 cagtattacg gaaaatacaa gtgcagtgag cgaaacgatc gaagatattt caaagagtgc
     2641 actgataaca tctgaaaaca tagaacagca gacggaaatg acaggctcca tt
//
LOCUS       DAOSYE010000258         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28926_length_2687_cov_3.542553, whole genome shotgun sequence.
ACCESSION   DAOSYE010000258 DAOSYE010000000
VERSION     DAOSYE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28926_length_2687_cov_3.542553"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1478)
                     /locus_tag="PLU43_05420"
     CDS             complement(261..1478)
                     /locus_tag="PLU43_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOO27883.1"
                     /translation="MKGIGNKLIGNEEMVPLEERIFNFSLLVSICLTAIGAGLDLFFY
                     SSTWTNLIFAGIWILTYYFSRFRGRFKEASVAGIVILIFAFFPYNWIYSSGSSGVLPY
                     YSVVLLVILCAVLKGKFRFFMILSFIAVEMLLICHDAYEAGSFFVQPASNTTGAELMI
                     HMGIILVSISIFIVVYSNVYEKEKARRETYASSIEEHYRQQLYYMKNLEEVIDKLRSE
                     RHDFNHYLGVIYGLLETKETEKAGTYVSQLVRTAEEYQNLVNIPYPIARAMLNYKLSD
                     AKEKHILLRLDIRVPAELELPEFDLVVILGNLLDNAVEANKEIDEQARYLALSIFYRP
                     NYLVIHTENPVSAEVTSAKEHKTTKSDVSEHGFGLRNIEYLTKKHDGFLKTSREHGIF
                     KADVALLIGTGGK"
     gene            complement(1475..2206)
                     /locus_tag="PLU43_05425"
     CDS             complement(1475..2206)
                     /locus_tag="PLU43_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO27884.1"
                     /translation="MNYTIGICDDCREQVELLAGYLKDCENGDNFYIVRETVPEAFME
                     TLATLKPDLVFLDIDMDGMNGIELGKKIRDLYETVVIVYVTGYEKYAYDAYQLRAFHY
                     LLKPVTKERFAEVLQEALAQHRKNAFEKKERTFSVRTKGETRILYYRDILYFEKISHK
                     IKIHTGSQDIYFYDNLYHLLEKLAADTFVQCHQGYIVNVDKIRAFRSSILYLEGDVRL
                     PVSRTFSRQIQKVLEKHLFSGEETI"
     gene            complement(2367..>2687)
                     /locus_tag="PLU43_05430"
     CDS             complement(2367..>2687)
                     /locus_tag="PLU43_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27885.1"
                     /translation="MRSMLSGIFLILFLGSGIGCGIGGAVAGHISAESLNKVYYDDMY
                     SNGIGNSEESEAVYADSPEDLTIAVICLLSAAAVISLGMLISANRMSQVLKQEPMKLL
                     TTEK"
BASE COUNT          771 a          651 c          456 g          809 t
ORIGIN      
        1 ttaccctgac atgcgtttaa tattttctct tcatttccct ttgttcctgt atctaccaaa
       61 atagaactct tttcctgtga tacgagataa cagttagaac cggctttgat ttgtataata
      121 tggtacatca gcttttccct ccgcttctct tattttattt aattgtctaa atagtttagt
      181 ttctaaactg cttaacaact attttaccat tatattccga tttatgtcaa tcattatttt
      241 cagtacaacg acttatgctg tcactttcct ccagttccga tcaaaagcgc aacatctgcc
      301 ttgaatatcc catgttccct ggacgtcttc agaaatccat catgcttttt tgtcagatat
      361 tcaatattgc gaaggccgaa tccatgctca gaaacatccg atttcgttgt tttatgctct
      421 tttgctgacg ttacttctgc gctcaccgga ttttccgtat gaataacaag ataattgggt
      481 ctgtaaaata tgcttaatgc gagatatcgg gcctgttcat cgatttcttt atttgcttcc
      541 accgcattgt ccaaaagatt gccgagaata acgacgagat caaattccgg aagctccagt
      601 tctgccggta ctctgatatc caatcggagc agaatatgct tctcttttgc atccgacaat
      661 ttataattaa gcattgcacg tgcgatcgga tatggaatat tcacaagatt ctgatattct
      721 tctgccgtcc tgacaagctg tgagacatat gttcctgcct tctctgtttc tttcgtctcc
      781 agcagaccgt aaatcacgcc aagataatga ttaaaatcat gccgttccga ccgcagctta
      841 tcgattactt cctccagatt tttcatataa taaagctgct gtcggtaatg ttcttctata
      901 gagctggcat aagtttctct ccgggctttt tctttttcat acacatttga ataaacaaca
      961 ataaaaatcg agatcgaaac aagtatgatc cccatatgaa tcataagttc cgcccccgtt
     1021 gtattactcg ccggctgcac aaaaaacgaa cctgcctcat atgcatcatg acaaataagc
     1081 agcatttcca ctgctataaa cgataaaatc ataaaaaaac gaaattttcc cttgaggacc
     1141 gcacacaaaa taacaagaag aacaacggaa tagtaaggca atacaccgct gcttccgctg
     1201 ctgtagatcc agttatatgg aaaaaaggca aagatcaaaa taacgattcc tgcaaccgac
     1261 gcttccttga agcggccccg aaaccgggaa aaataatagg tcagtatcca tatgccggca
     1321 aaaataagat tggtccatgt actggaatag aaaaaaaggt ccagtcccgc accgatcgca
     1381 gtcaggcaaa tcgaaactaa cagggaaaaa ttgaagattc gttcttcgag cggaaccatt
     1441 tcctcattgc cgattaattt atttccaatc cctttcatat cgtttcctct ccggaaaaaa
     1501 gatgtttttc caataccttc tgtatttgtc tggaaaacgt tctgctgacc ggcagcctga
     1561 cgtctccttc cagataaagt atactgctgc gaaacgccct gatcttgtct acattcacaa
     1621 tatatccctg atgacactgc acaaacgtat ctgcggcaag cttctccagt aaatgataca
     1681 aattgtcata aaaataaatg tcctgagaac ctgtatggat cttaatctta tgactgatct
     1741 tctcaaaata gagaatgtcc cgataataaa gaattctcgt ttcacctttc gtccggacag
     1801 aaaaagtccg ttcctttttt tcgaaagcat ttttccggtg ctgcgccagt gcttcctgca
     1861 ggacttcggc aaaccgttcc tttgttaccg gctttaaaag ataatgaaat gccctgagct
     1921 gatatgcgtc ataagcatat ttttcataac cggtaacata aacgatcaca acagtttcgt
     1981 atagatccct gatctttttt cctaattcga tcccattcat cccgtccata tcgatatcca
     2041 gaaatacaag atcaggcttc agcgtcgcaa gcgtttccat aaatgcttcc ggaacagttt
     2101 cacggacaat ataaaaattg tccccgttct cacaatcctt taaataaccg gcaagcagct
     2161 ccacctgctc ccggcagtcg tcacaaattc cgattgtata gttcatccgt actccttatt
     2221 ctttctgcct ttcgccgcct tgcgaaaagc ccgcctttat taatatactc ttaaatatta
     2281 tcttatcgta attaaaacaa aaaagaaatg ccgattgcat gatcggcatt tgaaataaca
     2341 tcatttgatg attttttcta aattattcac ttttccgttg tcagcagctt catcggttcc
     2401 tgcttgagaa cctgtgacat cctgttggcg gaaatcagca tgccaagtga aataactgct
     2461 gctgcagaca gcagacagat cacagcgatc gtcagatctt ccgggctgtc cgcataaaca
     2521 gcctccgact cttcactgtt tccgatgcca ttgctgtaca tgtcgtcata atacacttta
     2581 ttcaggcttt ctgcggaaat atgcccggcc actgctccgc cgataccgca tccgatgccg
     2641 cttccaagaa aaaggatcaa aaagattcca gagagcatag aacgcat
//
LOCUS       DAOSYE010000259         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28929_length_2687_cov_3.392857, whole genome shotgun sequence.
ACCESSION   DAOSYE010000259 DAOSYE010000000
VERSION     DAOSYE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28929_length_2687_cov_3.392857"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..882
                     /locus_tag="PLU43_05435"
     CDS             103..882
                     /locus_tag="PLU43_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27886.1"
                     /translation="MKRYKNILEIKDLCVYAGEKCLLSHLNFVIPDGEVHALLGPNGS
                     GKTSLMMTIMGFSGYEVTQGEILFKGEPITELAVCERARLGIAIAQQRPPTIAGVTLR
                     SIVSYALRETKDPEKKLAELAADVRMEQFLDRSINDGLSGGEIKRSELLQLLALSPAF
                     SMMDEPDSGVDIEALAMVGELTNRLFTPDEKRPVRRKAGLVITHNGNILKYLNIDKAH
                     IMYEGHIGCSGNPGIMLDKIGRYGYEECVRCMGGGDADEQE"
     gene            869..2014
                     /locus_tag="PLU43_05440"
     CDS             869..2014
                     /locus_tag="PLU43_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOO27887.1"
                     /translation="MNRSDKEAPALTAPENIKAEDRETMSRAGILFDRGRRSGTMIVR
                     DHNPVCLQVETKRFEMLPIAEALTRYEWLKEKYCFKAVPADYDEEVSACAAQEKPLGF
                     FIHVKKGAHVTLPCQTAMYMASENITQMVHNIVLLEEDSNLELITGCLTHHNVSSGLH
                     IAVEEHYVGKNAKLRSTMIHSWGPEVLVYPRTGTIVEEGGRYESNYISIRAAKKVFSD
                     PQTWLNGKGASAKYLTVVLGAPGSVINTEGNVYLNAEDTGAELVHRGVCTGGAMYQGG
                     LLIGNAPCRAHVDCAGMLLDQTGKGFIESVPGLRSHHSDARMSHEASIGKIAPEQVEY
                     LMSRGMEEREAISLMIRGFLGADIIGLGTELDAQIAEMVEIAGHGEE"
     gene            complement(2062..>2687)
                     /locus_tag="PLU43_05445"
     CDS             complement(2062..>2687)
                     /locus_tag="PLU43_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOO27888.1"
                     /translation="DTSLLKAAEAAEILRQKIEKTRHDDISCPVTASFGVTEFMHGET
                     EESWFKRVDYALFCSKKEGRNRVTAISWDTVIPFVQVKLDWKSEWESGIASIDEQHKR
                     LVLLGNRLLDEVFADAKSEKTAKALAELTEHIRFHFEDEVRVLQKFAYPAAKEHAGIH
                     NDLLKQTEDLTILYQNGTLKSPLFFHFFWMRSSSAIYLMKTACSFRI"
BASE COUNT          734 a          552 c          747 g          654 t
ORIGIN      
        1 ttatgatcag ccggcgggta atttacggca tcataagcgt aaaatttaat ccaatggtta
       61 gaaaaaacta accgcaggat cgaaagaagg tgatatcata cgatgaaaag gtataaaaat
      121 atacttgaaa ttaaagatct ttgtgtttat gcgggagaaa aatgcctgct cagtcatttg
      181 aattttgtga ttccggatgg agaggtgcat gcgctgttag gtccgaacgg cagcggaaaa
      241 acgtcactta tgatgacgat catgggtttt tccggttacg aagtgacgca gggagaaatt
      301 ttgtttaaag gagagccgat tacagagctt gcggtttgtg agcgcgcacg gcttggaatt
      361 gcgatcgcac agcagcggcc gccgacaatt gccggggtga cactgcgaag tattgtgtct
      421 tacgcactca gagaaacaaa ggatccggag aaaaaactgg ccgaactggc cgcagatgtt
      481 cggatggagc agtttttgga ccgcagtatc aacgatggat tatcgggcgg tgagatcaaa
      541 cgctcggagc ttttgcagct tctggcattg tcaccggcat tttccatgat ggacgagcct
      601 gattcaggcg ttgatatcga ggcgcttgcc atggtaggag aactgacgaa ccggctgttt
      661 acgccggatg aaaagaggcc ggtaagacgg aaggcaggtc tggttattac acataatggg
      721 aatattctca aatatttaaa catagacaag gcgcatatta tgtatgaagg ccatatcggc
      781 tgctccggga atccgggaat catgctggac aagatcggca ggtatgggta tgaagaatgc
      841 gttcgctgta tgggaggagg tgacgcggat gaacaggagt gataaagaag cgccggcatt
      901 gacggcgccg gaaaatataa aagctgaaga cagggaaaca atgtccagag ccgggattct
      961 gtttgacaga ggccggcgtt caggcacaat gatcgtacgt gatcacaatc ctgtctgcct
     1021 gcaagtcgaa acaaagagat ttgagatgct tccgatcgca gaggccctta cacgctatga
     1081 atggcttaag gagaagtatt gtttcaaagc ggtgcctgct gattatgatg aggaagtatc
     1141 tgcctgtgca gcgcaggaaa agccgctggg attttttatt catgtcaaaa aaggtgcaca
     1201 tgtaacgctg ccatgccaga cggcaatgta tatggcgagt gaaaacatca cgcagatggt
     1261 gcataatatc gtgctgttgg aagaggattc gaatctggaa ctgattacag gctgcttgac
     1321 gcaccacaat gtcagcagtg gtctgcatat tgcggtagag gagcattatg tcggaaaaaa
     1381 tgcaaagctg agaagtacga tgatccattc gtggggtccg gaagtgctcg tatatccacg
     1441 gaccggaacg atcgtggaag aaggaggacg gtatgagagc aactatattt ctatccgcgc
     1501 cgcaaaaaaa gttttcagcg atccgcagac atggctgaac ggaaaaggag catctgcgaa
     1561 atacctgact gtagtgttag gagcgccggg atcggtaatc aatacggaag ggaacgttta
     1621 tctcaatgcg gaagataccg gagcggagct tgtacatcgt ggagtctgta ccggaggagc
     1681 catgtatcag ggaggtctgc tgatcggaaa cgcaccgtgc agagcacatg tagattgtgc
     1741 gggcatgctg cttgatcaga ccggaaaagg atttatcgag tcggtgccgg gtcttagatc
     1801 acatcattcc gacgcgagga tgagtcatga agcaagcatc ggtaaaatcg caccggagca
     1861 ggtggagtac ctgatgtccc gcggaatgga agagcgggag gcgatctcgc ttatgatcag
     1921 aggattttta ggcgcagata tcattggtct tggaacagag ctggatgcgc agatcgcaga
     1981 aatggtcgaa attgccgggc acggagaaga atgatctgcc ggccggctag tcgttcccgg
     2041 aatctttttg gcagcagtct ttcagatacg gaaagaacag gcggtcttca ttaaataaat
     2101 ggccgacgac gacctcatcc agaagaaatg aaaaaataac ggggatttta aggttccgtt
     2161 ctgatatagg atcgtaaggt cctccgtttg ctttaaaagg tcattatgaa ttccggcatg
     2221 ctctttagca gcaggataag caaacttttg cagaacacgc acttcgtctt caaaatgaaa
     2281 tcttatatgt tctgtcagtt ctgccagtgc ttttgcggtt ttttcgcttt ttgcatcggc
     2341 aaagacctca tccaggaggc ggttcccaag aagcacaagt cttttatgct gttcgtcgat
     2401 cgatgcgatc ccgctttccc attcgctttt ccaatccagc tttacctgaa caaacggaat
     2461 cactgtatcc cagctgatgg cagtcacacg atttcggccc tctttttttg aacaaaacag
     2521 tgcgtaatcc acgcgtttga accagctttc ttctgtttcc ccatgcatga attctgtaac
     2581 accaaaactg gccgttaccg ggcagctgat gtcgtcatgc ctggtttttt cgattttttg
     2641 gcgtaagatt tcggcagcct cggcagcttt taacaaagag gtgtccg
//
LOCUS       DAOSYE010000260         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28946_length_2686_cov_3.330293, whole genome shotgun sequence.
ACCESSION   DAOSYE010000260 DAOSYE010000000
VERSION     DAOSYE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28946_length_2686_cov_3.330293"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="PLU43_05450"
     CDS             complement(<1..108)
                     /locus_tag="PLU43_05450"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOO27889.1"
                     /translation="MLVSATEMLKKAKAGHYAVRQFNINNLEWTKAVLLT"
     gene            complement(190..1080)
                     /locus_tag="PLU43_05455"
     CDS             complement(190..1080)
                     /locus_tag="PLU43_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOO27890.1"
                     /translation="MKKKKLLFICNAHAGKGLIKNKLVGILDIFAQAGYETTIHLTQE
                     QGDAISAVKEKEEGFALVVCSGGDGTLDEVVTGMMQSEENIPIGYIPAGSTNDFARSL
                     HIPIDMGKAAGIAVSGNYYACDTGSFNDDFFIYIAAFGIFTEVSYETSQEVKNVLGHM
                     AYILNGMRKLSAIKSYHLKVKTKELDYEDDYIFGMVTNSFSVGGFKSITGKEVRLDDG
                     LFEVTLIRRPKNALELQAILTSLVTEETDETYMHTFKTASVTMFSEEKIPWTLDGEFG
                     GDWGKVMIKNNQKAIRIAVP"
     gene            complement(1085..2278)
                     /locus_tag="PLU43_05460"
     CDS             complement(1085..2278)
                     /locus_tag="PLU43_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27891.1"
                     /translation="MKLFRVYRKVLKKNLPVIFAYMVILYAIILVMIFQVRSNANSYE
                     EDRITVVLINEDVTNEFTRGLKQYLGERVNLIDNSLYSGNIQSALFYQTADYIIKLPK
                     GFYEELYNYENDKGPVIYKYTAASTYAGKVMDKILKEYCDWWLAYREMYPDMSEQEIL
                     EKVTETMNMQSSLLYSNRLQISASQKNMYYFFNYASYSIMGIIGMGVVSALLLLGKSK
                     VYERTAVSPVAKSQIELELLLYNTGFAVLIWAAHVLGAMALFHESFFTWTGFFMCMNT
                     FALSMVSVGISYLMLCFVNSRGGIGIFVNLIVFAMCFISGTFIPQYLLDTNVRSAAVF
                     IPVFWYIRANDLINETGALSLLTLTDTLTDLIRLIGTQYLFAVMFFCAAMFITKQRGE
                     TVIGG"
     gene            complement(2265..>2686)
                     /locus_tag="PLU43_05465"
     CDS             complement(2265..>2686)
                     /locus_tag="PLU43_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27892.1"
                     /translation="VTVSYLYMLLILRIPFRMPFGYVLLIHAVGVFVSMLFGVFAGTG
                     DRLHLSAKLGIITAMIIVGAYFSGLMDIHVRVSTQEQILFIRFINPCTLITDAYYALY
                     QIDMQTLWKSLAALLTIGLLSVLLTILDAGEDRHETV"
BASE COUNT          795 a          679 c          444 g          768 t
ORIGIN      
        1 cgttaacaga acagctttgg tccactccag attattgatg ttaaactgcc ttaccgcata
       61 atggccggcc tttgcttttt ttaacatttc agttgctgaa actaacattt tgtttacctc
      121 cgttttttta ttctatttct ttatatcaca aaatacagaa aatgaaaaca taaaatatgg
      181 ctcattttgt taaggtactg cgatccgtat cgctttttgg ttgtttttta tcattacttt
      241 cccccagtca cctccgaatt ccccatccag tgtccatgga attttttctt cggaaaacat
      301 tgtcacggaa gctgttttaa acgtatgcat ataagtttca tccgtttcct cggttacaag
      361 cgaggtcaga atcgcctgca gctccagcgc atttttaggc cttctgatca gggttacttc
      421 aaacaatccg tcatccagcc ttacctcttt tcctgtgata cttttaaatc cgccgacgga
      481 aaacgaattc gtaaccatcc caaatatata atcgtcttca tagtccagct cttttgtttt
      541 tacttttaaa tgatacgact tgatcgctga aagctttctc ataccattca aaatatatgc
      601 catatggcca agtacatttt ttacttcctg gctcgtctca taagagactt ccgtaaaaat
      661 accgaatgcc gcaatataaa taaaaaaatc atcattaaaa gaaccggtat cacatgcata
      721 ataattgccg ctcaccgcaa taccggctgc ctttcccata tcgatcggga tatgcagact
      781 ccttgcaaaa tcatttgtac tgccggccgg aatatatcca atcggaatat tctcttcgct
      841 ctgcatcata cctgtcacga cttcatccag cgtcccatcg cctccgctgc acacaacaag
      901 cgcaaagcct tcttcttttt ccttaaccgc actgatcgca tctccctgct cctgcgtcaa
      961 atggatcgtc gtttcataac ctgcctgcgc aaaaatatcc aaaattccaa caagtttgtt
     1021 ttttatcaga cctttccccg catgtgcatt acagataaac aacaattttt tctttttcat
     1081 cctatcagcc tcctatcact gtttctcccc tttgttttgt aataaacatc gccgcgcaga
     1141 aaaacatgac cgcaaacaaa tactgcgttc cgatcagcct gatcagatct gtcagtgtat
     1201 ctgtcagtgt caggagcgaa agtgccccgg tttcattgat cagatcattc gcacggatat
     1261 accagaaaac aggaataaat acggcggcag aacggacatt ggtatccagc agatactgcg
     1321 gaataaacgt tccgctgata aaacacatgg caaaaacaat cagattcaca aaaataccaa
     1381 tgcctccgcg gctgttcaca aagcacagca tcaggtaaga gattccaacc gaaaccattg
     1441 acagtgcaaa tgtattcata cacataaaaa atccggtcca tgtgaaaaaa ctttcatgaa
     1501 acagcgccat ggctcccagt acatgagcag cccagatcaa tacggcaaac ccggtattat
     1561 aaagcagcag ttcaagctct atctgacttt ttgcaacagg cgaaactgct gttctttcat
     1621 atacctttga tttgccaaga agcaaaagtg cacttacaac gcccatcccg atgatcccca
     1681 taatggaata cgacgcataa ttaaaaaagt aatacatatt tttctgactc gcactgatct
     1741 gcagccggtt agaatacagc agactgctct gcatattcat tgtttccgta accttttcca
     1801 gaatctcctg ttcactcata tccggataca tttcgcggta agcaagccac cagtcacaat
     1861 attccttcag tattttatcc ataaccttcc ctgcatacgt ggatgctgcc gtatatttat
     1921 aaataacggg acctttgtca ttttcataat tatacagctc ttcataaaat cctttcggca
     1981 gtttgataat ataatcagct gtctgataaa ataatgcact ctggatattc ccgctgtaca
     2041 gactgttatc gatcagattt acccgctcac caagatactg cttcaatccg cgtgtaaact
     2101 catttgtcac atcctcattg atcagtacga ccgttatcct gtcttcctca taagaatttg
     2161 cattgcttct tacctgaaag atcatcacca gaatgatcgc ataaaggatc accatataag
     2221 cgaatatcac cggcagattt ttcttcagaa cttttctgta gaccctaaac agtttcatgt
     2281 ctgtcctccc ctgcatcaag aatagtcagc agcacggaaa gcagtccgat cgtaagcagc
     2341 gcggccagac ttttccataa cgtctgcata tcaatctgat acagcgcata ataagcgtcc
     2401 gtaatcagcg tgcacggatt gataaacctg ataaacagaa tctgttcctg tgtggaaacg
     2461 cgtacatgga tatccatcag accggaaaaa tatgcaccga caatgatcat cgccgttata
     2521 atgccaagct ttgcggataa atgcagtctg tcgcccgttc ccgcaaaaac tccgaaaagc
     2581 atactgacaa aaacaccgac cgcatggatg agcagtacat atccaaaagg catccggaat
     2641 ggtattctaa gaatcagcag catatataaa taagaaactg tcacaa
//
LOCUS       DAOSYE010000261         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28956_length_2686_cov_2.320030, whole genome shotgun sequence.
ACCESSION   DAOSYE010000261 DAOSYE010000000
VERSION     DAOSYE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28956_length_2686_cov_2.320030"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLU43_05470"
     CDS             complement(<1..288)
                     /locus_tag="PLU43_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO27893.1"
                     /translation="MKILLAILIFCIIVISHELGHFLIARANGIAVKEFCIGFGPTLC
                     GFTKNGTKYSIKPIPLGGACIFDDEDTENPSESSFLKASVWARIATIAAGPM"
     gene            complement(291..1433)
                     /gene="dxr"
                     /locus_tag="PLU43_05475"
     CDS             complement(291..1433)
                     /gene="dxr"
                     /locus_tag="PLU43_05475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOO27894.1"
                     /translation="MKEIAVFGSTGSIGTQTLDIVRANKDLKVSVIAAGSNIALAERQ
                     IREFLPKLCVIYDEAAADRLKKNIRDLSTKVLCGMEGMLEAAVLPQTDIVVTAMVGMI
                     GIRPTIAAIKAKKDIALANKETLVTAGHLIMPLAKEQGVSILPIDSEHSAIFQSLNGE
                     NPERISKILLTASGGPFLGRKKSELAGITVEDALRHPNWSMGRKITVDSATLVNKGLE
                     VMEARWLFGVGLDRIQVVVHPQSIIHSMVEYIDGAIIAQLGMPDMKLPIQYALFYPDR
                     KMLDTKHVDFYSLQQMTFMEPDMETFSGLKMAFEAAAAGGTMPTVFNAANEKAVGLFL
                     DKKIGFLDIYDMIRQSMEHHKTLQDPDVAQILAAEQETYEFIESRW"
     gene            complement(1458..2285)
                     /locus_tag="PLU43_05480"
     CDS             complement(1458..2285)
                     /locus_tag="PLU43_05480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOO27895.1"
                     /translation="MFFTRLASSVVLLILTIAVNIIGGPVLVCELAVISLIGLYEFNR
                     AVGVRTKEKGVNLLEAVMILGTIVFYVTGYLVYDNLERYLLLIIVIVFVLLMAVYVFT
                     FPKYEASQVIHGFFGFIYVPVMLSFIYFTRRLLYGEYTVWLIFIGSWICDTCAYCTGM
                     LFGKHKLAPVLSPKKSVEGSVGGVIGAAAVAALFVYFLVVRMTPSPEIIGTFIIIAAV
                     GAVVSQVGDLAASAIKRNHNIKDYGNLIPGHGGILDRFDSVIFTAPMIYFLVVIFIH"
     gene            complement(2287..>2686)
                     /gene="uppS"
                     /locus_tag="PLU43_05485"
     CDS             complement(2287..>2686)
                     /gene="uppS"
                     /locus_tag="PLU43_05485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOO27896.1"
                     /translation="PDRQERSAQLEEATKDNDGLHFQVALNYGSQDEMLRAMNHIRRD
                     MKDGTIPEGEITKELFESYLDTAGIPDPDLLIRTSGEQRLSNFLLWQLAYTEFYTADV
                     HWPDFSKEELIRAVEAYSGRERRFGGVKEE"
BASE COUNT          734 a          733 c          484 g          735 t
ORIGIN      
        1 catcggtccg gcagcgatcg tcgctatcct cgcccagaca cttgctttta aaaaggaact
       61 ttccgatgga ttttccgtat cttcatcatc aaaaatacaa gcgcctccca acgggatcgg
      121 tttaatgctg tatttcgtcc cattcttcgt aaatccacat aatgtcggac caaatccaat
      181 gcaaaattcc ttaactgcga tcccatttgc cctggctatt aaaaaatgcc caagttcgtg
      241 tgatatgact ataatgcaga aaatcaatat tgccagtaag atcttcataa ttaccacctg
      301 ctctcaataa attcatatgt ttcctgctct gctgcaagaa tctgcgccac atccggatcc
      361 tgcagtgttt tatgatgttc cattgactgc ctgatcatat cataaatatc cagaaagcca
      421 atttttttat ctaaaaacaa gccgactgct ttttcatttg cggcgttaaa caccgtaggc
      481 atcgtccctc ctgcagcagc tgcctcaaaa gccattttaa ggcctgagaa tgtctccata
      541 tcaggctcca taaatgtcat ctgctgaagc gaataaaagt ccacatgctt cgtgtcaagc
      601 atttttctgt caggataaaa aagcgcatac tggatcggca gcttcatatc cggcattcca
      661 agctgcgcta taatggctcc gtcaatatac tccaccatcg aatgaataat actctgtgga
      721 tgcacaacga cctgaatacg gtcaagtccg acaccaaaaa gccagcgcgc ctccatgact
      781 tcaagtcctt tattaacaag tgttgccgaa tccaccgtga tttttctgcc catcgaccag
      841 tttggatgcc gcagcgcatc ctctaccgta attcctgcca gttcactctt tttcctgcca
      901 aggaatggtc cgcctgatgc cgtcagcagg atcttgctga tccgttccgg attttctccg
      961 ttaagtgact gaaaaatagc gctgtgttca ctgtctatcg gcagaatgga tacgccctgt
     1021 tcctttgcga gcggcatgat cagatgacct gccgttacaa gtgtttcctt attggcaagc
     1081 gcaatatcct tttttgcctt aattgcagcg atcgtcggcc tgataccgat catccccacc
     1141 attgcggtaa cgactatgtc tgtctgcgga agaaccgcag cttccagcat tccttccatc
     1201 ccgcagagta ctttggtaga aagatccctg atattttttt taagcctgtc tgctgccgcc
     1261 tcgtcataga tcacgcacag cttcggtaaa aactccctga tctgcctctc tgccagtgca
     1321 atattggaac cggcagctat aacggacact ttcaaatcct tatttgccct tacaatatca
     1381 agcgtctgcg tcccaataga acctgtagag ccaaataccg ctatttcttt cataataatc
     1441 gctcctaaac ccgtaattta atgtataaaa ataactacca gaaaatagat catgggtgcc
     1501 gtaaatatca cactgtcaaa gcggtccaaa atgcccccgt gtccaggtat cagatttccg
     1561 taatctttga tgttatgatt ccgcttaatt gccgaagctg ccagatcccc aacctgagaa
     1621 acgaccgcac caactgctgc tatgataata aacgttccga taatttccgg actcggcgtc
     1681 atccggacaa ccagaaaata tacaaacaga gccgcaacgg ccgctgcacc gataactccg
     1741 ccgacagaac cttctacgga cttctttgga cttaacaccg gcgcaagctt atgctttcca
     1801 aacagcatcc ctgtacagta tgcacatgta tcgcagatcc atgagccgat aaaaatcagc
     1861 cataccgtat attccccgta aagcagtctt cttgtaaaat atatgaacga aagcatgacc
     1921 ggtacatata taaatccaaa aaatccgtga attacctgtg atgcttcata tttaggaaac
     1981 gtaaatacat atactgccat caaaagtaca aatacgatca caatgataag aagaagatac
     2041 cgttccaggt tgtcataaac cagatatcct gttacataaa atacgatcgt gcccaatatc
     2101 ataactgctt ccagcagatt tacacctttt tcttttgttc tgacaccgac agcacgatta
     2161 aactcataca gcccgatcag ggaaatcact gccagttcgc ataccagaac cggtccccct
     2221 attatattta ccgcaattgt cagtatgaga agcacaacac tgcttgccaa tcttgtaaaa
     2281 aacatattac tcctccttca ctcccccaaa acgtctctct ctgccgctgt aggcttcaac
     2341 tgccctgatc agctcctctt ttgaaaaatc aggccagtgt acatccgcag tatagaattc
     2401 cgtataggca agctgccata acaaaaagtt ggaaagtctc tgctccccgc tcgttcggat
     2461 caatagatcc gggtctggaa tacctgccgt atccagatag ctttcaaaca gttcttttgt
     2521 aatttctcct tccgggatcg ttccgtcctt catatcgcgc cgaatatgat tcattgcccg
     2581 cagcatttca tcctgactgc catagttcag cgccacctga aaatgcagcc cgtcattgtc
     2641 tttcgttgcc tcttcaagct gtgcgctacg ctcctgccga tccggt
//
LOCUS       DAOSYE010000262         2684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_28984_length_2684_cov_3.245340, whole genome shotgun sequence.
ACCESSION   DAOSYE010000262 DAOSYE010000000
VERSION     DAOSYE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28984_length_2684_cov_3.245340"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2684)
                     /locus_tag="PLU43_05490"
     CDS             complement(<1..>2684)
                     /locus_tag="PLU43_05490"
                     /inference="COORDINATES: protein motif:HMM:NF037639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HOO27897.1"
                     /translation="HIDKATTDPSTGELVGTYTTDSSGEIVVDSLEWGNYYFMETSAP
                     AGYTIETNSGGYNRPYNFTIDSENVTTVQLVEVENERESGSVEIEKISKNDNSKLDGV
                     VFKLYKDEVKDDNLIATLTTGSDGKAYKDGLAWGDYYLVEVSTKDGYILDSTPRTFTI
                     DGTHLSKSYTGNAAIENEKKQGYVELEKKDETTSAPMSGVEFKLYKGESPSGSYIGTY
                     TTDSNGKIEKETIGALDVGKYYFEETTPTGYETYTGNLAFEIEDADQVVYFTGTNAII
                     NTPQEGSVKLKKVDKDSRAVLAGAEFELHAVDVSNAGVLALLKSLFGGSATYGTYTTN
                     ENGEIYVSGLPWGSYYFIETKAPQGYTITDEGKQYPFTISAGNASETIDIGTIENTQQ
                     KGSIKLTKVDSETGALLVGAEFKLYLKGDTSDTDVSSTYGATNGVFTTAQDGTITVTD
                     LDWGTYYFKETKAPDGYEAISDTNVIQSAPVTISAANADATTNLMTQQTVEVDNMKGY
                     GYVRLKKTFDGTQPSDLSGIGFTLVNKTTGVAVGSYTTDTSGMITADIIGKLPYGEYY
                     FTETSVPVGVSYAVNTSPLEFSITESCAIADAVEYTFTNSEILASAKFSKTDAATTEI
                     IAGIKFGVYNADDDTLLTTVESNAQGIVTVTGRPIGSYYFLENAASAEAAGYVPNTTK
                     YYFDVTAADKQSEDASGTKTEKFVDVYTKDASGSIAVITTVTNTKENGSIELVKFGVD
                     SDNNKTKLSLTDAEFELYKDGVLYKTAAELKAMYSAGKLVVTDLPWGTYYFKETKAPA
                     GYALPDGTAANTNSVTIDGTTVTASLETPLTCQITDDTIKLYISKREIAGSEELAGAK
                     MELYLADDSGSMTGSALASWTSGTTAKLFEISDELTGG"
BASE COUNT          625 a          711 c          514 g          834 t
ORIGIN      
        1 gtccgcctgt caattcatcg ctgatttcaa acaatttcgc cgtcgtaccg gaagtccagc
       61 ttgccaatgc actgccggtc atgcttccgc tgtcgtcagc caggtaaagc tccatttttg
      121 cgccggcaag ttcttcggaa cctgcaattt cccgtttgga aatataaagc ttgatcgtat
      181 cgtcagttat ctgacaggtg agcggcgttt ccagtgaagc cgttacggtt gttccgtcga
      241 tcgtaaccga atttgtattt gcagcggttc cgtcaggaag cgcatatcct gcaggtgctt
      301 ttgtctcttt gaaataataa gtgccccacg gaagatcagt tacaacgagc ttccctgcac
      361 tgtacattgc tttcaactcc gctgccgtct tatataatac accgtcctta tagagctcaa
      421 attccgcatc tgtcaaagac agctttgttt tgttattgtc actgtccaca ccaaacttca
      481 ctaattcaat ggaaccattt tccttcgtat tcgttaccgt tgtaatcaca gctatactac
      541 cggacgcatc tttcgtataa acatctacaa atttctctgt ttttgttccg gacgcatcct
      601 ctgactgctt atctgcggca gttacgtcga aataatattt tgttgtattc ggcacatatc
      661 cggcagcttc tgccgaagca gcgttctcaa ggaaataata actgccgatc ggacgtcccg
      721 ttaccgttac gatgccttgt gcattagact ccactgttgt aagaagcgta tcgtcgtctg
      781 cgttataaac accgaattta atacctgcaa taatctcggt cgtagccgca tctgttttgc
      841 tgaatttcgc cgatgccagt atctcactgt ttgtaaacgt atattcaact gcatccgcaa
      901 tcgcacagct ctccgtgatg ctgaactcca gtggtgatgt attgaccgca tagcttactc
      961 ctaccggtac gcttgtctcc gtaaagtaat actctccgta aggcagtttc ccgataatat
     1021 cggccgtaat cattccactc gtatcggttg tataacttcc aacagcaaca ccggttgtct
     1081 tatttacaag cgtgaaacca ataccgctca aatccgacgg ctgtgttcca tcaaacgtct
     1141 ttttcagcct gacatagcca tagcctttca tattgtccac ttctaccgtc tgctgtgtca
     1201 tgagattcgt tgtcgcatca gcatttgccg cactgatcgt tacaggcgca gactgtatta
     1261 cgttcgtatc cgatatggct tcatagccat ccggcgcctt tgtctcctta aaatagtaag
     1321 taccccagtc aagatccgtt accgtaatgg ttccgtcctg cgctgtcgta aacacaccgt
     1381 tcgttgcacc ataagttgac gaaacatccg tatccgaagt atcacctttc agatagagtt
     1441 taaattccgc tccgacaagc agtgcgcctg tctcactgtc tactttggtc agcttgatcg
     1501 aacctttctg ctgcgtattt tcaatcgtac caatatcaat cgtttccgat gcattgccag
     1561 cacttatcgt aaacggatac tgcttaccct catcggtgat cgtatagccc tgcggtgcct
     1621 ttgtctcaat gaagtaatag gatccccacg gcagaccaga tacatagatc tccccatttt
     1681 catttgtcgt ataggtaccg taagttgcac ttccgccaaa taatgacttc aataacgcaa
     1741 gtactccggc attgctgacg tcaaccgcat gcagttcaaa ttctgccccg gcaagcactg
     1801 ccctgctgtc tttatcaacc ttcttaagct tgacagagcc ttcttgaggt gtattaatga
     1861 tcgcattcgt tcctgtgaaa taaaccacct gatcggcatc ttcaatttca aaggcaagat
     1921 ttccggtata tgtctcatat cctgtcggcg ttgtctcttc aaaataatat ttgccgacat
     1981 cgagtgcccc gatcgtctct ttctcgatct taccgttact gtccgtagta tacgtaccaa
     2041 tatagctgcc gcttggagat tctcccttat aaagtttgaa ttctacgccg gacattggcg
     2101 cgcttgtcgt ttcatctttt ttctcaagtt ctacatagcc ctgtttcttt tcattctcaa
     2161 tcgccgcatt gccggtatat gattttgaca aatgcgtacc gtcgatcgta aaggttcgcg
     2221 gggtcgaatc taatatatag ccgtcctttg tggaaacttc gacgagataa taatcacccc
     2281 atgcaagacc gtccttatat gcttttccat ccgaacctgt tgtcagcgtt gcaatcagat
     2341 tgtcatcttt cacttcatcc ttgtacagct taaacacaac accgtcaagc ttgctgttat
     2401 cattcttgga aattttctct atttctacac tgccgctttc ccgttcgttt tcaacttcca
     2461 caagctgcac tgtcgtaaca ttttcagaat cgatcgtaaa gttgtacggc ctgttgtatc
     2521 cgcctgaatt tgtttcgatc gtatagccgg ccggcgcact tgtttccata aagtaatagt
     2581 tgccccactc aaggctgtca acaacaattt ctccagaaga atccgtcgta taagtaccga
     2641 ccagttctcc tgttgaagga tccgttgtcg ctttatcaat atgg
//
LOCUS       DAOSYE010000263         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29006_length_2683_cov_3.377473, whole genome shotgun sequence.
ACCESSION   DAOSYE010000263 DAOSYE010000000
VERSION     DAOSYE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29006_length_2683_cov_3.377473"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..537
                     /gene="rpsE"
                     /locus_tag="PLU43_05495"
     CDS             28..537
                     /gene="rpsE"
                     /locus_tag="PLU43_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOO27898.1"
                     /translation="MKRTIIDAGSLELNEKVVSIKRVTKVVKGGRNMRFTALVVVGDG
                     NGHVGAGLGKATEIPEAIRKGKEDAMKKLITVALDESSSITHDYSGKFGGASVLLKRA
                     PEGTGVIAGGPARSVCELAGIKNIRTKSLGSNNKQNVVLATIEALSQLKTPEEVARLR
                     GKSIEEIVG"
     gene            551..736
                     /gene="rpmD"
                     /locus_tag="PLU43_05500"
     CDS             551..736
                     /gene="rpmD"
                     /locus_tag="PLU43_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOO27899.1"
                     /translation="MADKRIKVTLVKSVIGAIPKHRATVAALGLKKTGRSVELPDNDA
                     VRGMVKQVCHLVKVEEI"
     gene            759..1199
                     /gene="rplO"
                     /locus_tag="PLU43_05505"
     CDS             759..1199
                     /gene="rplO"
                     /locus_tag="PLU43_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOO27900.1"
                     /translation="MELSNLKPAEGSKHSDNFRRGRGHGSGNGKTAGKGHKGQKARSG
                     APRVGFEGGQMPLYRRLPKRGFKNRNSKEIIAVNMSVLEMFEDGATVSVDTLKEKGIV
                     KNPRDGVKILGNGELTKKLTVQANAFSASAKEKIEALGGKAEVI"
     gene            1199..2512
                     /gene="secY"
                     /locus_tag="PLU43_05510"
     CDS             1199..2512
                     /gene="secY"
                     /locus_tag="PLU43_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO27901.1"
                     /translation="MLNTLQNAFKIKEIRKKLIFTFMMLVVIRLGSEMPVPGVSRTFF
                     SSWFSQQTGDAFNFLDAFTGGSFLKMSVFALNITPYITSSIIMQLLTIAIPKLEEMQR
                     EGEDGRKKITEITRYVTIGLSLIESAAMAIGFGNQGLLADYNAINVITVIAALTAGSA
                     FLMWIGEQITENGVGNGISIVLTINIISRIPQDFSSLYEQFMKGKSFATAVMAAIIIL
                     AVVLGTVVLTVILNGGERRIPVQYAKKVQGRKVVGGQSSHIPLKVNTGGVIPIIFASS
                     LLQLPIVISSLTNMNETGVWATILHTLNSGNWFKTATPVYSFGALVYVVLVIFFAYFY
                     TSITFNPLEVANNMKKQGGFIPGIRPGKPTSDYLTKILNYIIFIGAVGLIIVALIPII
                     FNGLFGADVSFGGTSLIIIVGVILETVKQIESQMLVRNYKGFLND"
     gene            2602..>2683
                     /locus_tag="PLU43_05515"
     CDS             2602..>2683
                     /locus_tag="PLU43_05515"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461292.1"
                     /note="essential enzyme that recycles AMP in active cells;
                     converts ATP and AMP to two molecules of ADP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOO27902.1"
                     /translation="MKIIMLGAPGAGKGTQAKQIAGKYGIP"
BASE COUNT          799 a          460 c          699 g          725 t
ORIGIN      
        1 ttagaattct aggaaggaga agaacacatg aaacgtacaa tcatagatgc aggcagctta
       61 gaactgaatg agaaagtagt gtcgatcaag cgtgtaacaa aagttgtgaa gggcggacgt
      121 aatatgcgtt ttacagcatt ggtcgttgtc ggagacggga acggacatgt tggtgcaggc
      181 ttgggaaaag ctacggaaat cccggaagca atccgtaaag gaaaagaaga tgcgatgaag
      241 aagctgatca ccgttgcatt ggatgaaagc agcagtatta cacatgatta cagtggtaag
      301 ttcggcggcg cttccgttct tttgaaaaga gctccggaag gtaccggtgt tattgcgggc
      361 ggtccggcac gtagcgtatg cgagcttgca ggtattaaaa atattcgtac aaaatcgctt
      421 ggttccaata acaagcagaa cgtagtatta gctacgattg aagcattaag tcagttaaag
      481 acgccggaag aggtagcacg tcttcgcggg aaatccattg aggaaatcgt cggctaagga
      541 ggcaggtaag atggcagata agagaattaa agttacttta gttaaatcgg taatcggcgc
      601 gattcctaag cacagggcga ctgttgcggc attagggtta aagaagacag gcagaagcgt
      661 ggaacttccg gataatgatg cggtaagagg aatggtgaag caggtttgcc atttggttaa
      721 ggtagaagaa atctaatata cagataggag gtgtcaagat ggaattatct aatttaaagc
      781 cggctgaagg ctcaaagcat agtgataatt ttagaagagg tcgtggacac ggttcgggaa
      841 acggtaagac agccggaaaa ggacataaag gtcaaaaggc tcgttcaggt gcgccgagag
      901 ttggctttga aggcggacag atgccattat acagaagact tccgaagaga ggctttaaga
      961 acaggaattc aaaagaaatt atagcagtaa acatgtctgt tcttgaaatg tttgaagatg
     1021 gcgctacagt ttccgttgat actttgaagg aaaaaggaat cgtaaagaac cccagagatg
     1081 gtgttaaaat tcttgggaac ggagagttaa ctaagaagct tacggtacag gcaaatgcat
     1141 tcagtgcatc tgcaaaagag aaaatagaag ctcttggtgg aaaagcagag gtgatctaat
     1201 gctcaataca ctgcaaaacg catttaaaat caaagaaatc agaaaaaaac ttatttttac
     1261 atttatgatg ctggttgtaa tcagacttgg ctctgaaatg ccggtgccag gtgtctccag
     1321 aacgtttttt tccagctggt tttcgcagca gacgggtgat gcttttaact ttcttgacgc
     1381 atttacgggt ggctcgtttt tgaaaatgtc cgtgtttgca ttgaatatta caccgtatat
     1441 tacttcttcc attatcatgc agcttcttac gattgcaatt ccgaaactgg aggagatgca
     1501 gagagaagga gaagacggaa gaaaaaagat cacggagatc acaagatatg ttacgatcgg
     1561 cctttctctg atcgaatcgg cagcaatggc gatcggtttt ggaaatcagg gattgcttgc
     1621 ggattataat gcaattaacg ttattaccgt tattgcggca ctgactgccg gctccgcatt
     1681 tttgatgtgg atcggtgaac agattacgga aaatggtgtt ggcaatggta tttccatagt
     1741 acttaccatt aatatcatct cgagaattcc acaggatttt tcttctcttt atgagcagtt
     1801 tatgaaaggg aaatcttttg caacggcagt aatggcagct atcattattt tagcggttgt
     1861 tcttggtacg gttgttctga ctgtcatttt gaatggcgga gaaagaagga tcccggtaca
     1921 gtatgcaaaa aaggtacagg gaagaaaagt agtaggcgga cagtcctctc atattccttt
     1981 aaaggtaaat acgggcggtg ttattccgat catctttgct tcctcgctgc tgcagctgcc
     2041 gatcgttatt tcttctttga ccaatatgaa tgaaaccggt gtgtgggcaa cgatcctgca
     2101 tacattgaat tcaggcaact ggtttaagac ggcgacgccg gtgtattcgt tcggcgcact
     2161 ggtctatgtc gtgctcgtaa tcttttttgc atatttctat acgtcgatca catttaatcc
     2221 gttggaagta gcaaataata tgaaaaagca gggcgggttt attccgggca tcagaccggg
     2281 caagcctacc agcgattatt taacgaagat tctgaattat attattttta tcggtgcggt
     2341 aggtctgatc atcgtagctc tgataccgat catattcaat ggactgttcg gtgcggatgt
     2401 ttcatttgga ggaacgtccc tcatcattat cgtaggggtt attcttgaga cggtgaagca
     2461 gatcgagtct cagatgttag tacggaatta taaaggattt ttgaacgatt aagcacagaa
     2521 aaagacaagg ctttatgcct tgtcttaccg tgcttgcatt gcttaacagt agtgttgttt
     2581 cataatatag gaggaatcat aatgaagatt attatgttgg gcgcacccgg cgcaggaaaa
     2641 ggaactcagg caaagcagat tgccggtaag tatgggattc ctc
//
LOCUS       DAOSYE010000264         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29233_length_2670_cov_3.647419, whole genome shotgun sequence.
ACCESSION   DAOSYE010000264 DAOSYE010000000
VERSION     DAOSYE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29233_length_2670_cov_3.647419"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLU43_05520"
     CDS             complement(<1..203)
                     /locus_tag="PLU43_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOO27903.1"
                     /translation="MTSKQRAYLKGLASTMEIVVHIGKASLTPEIVSSVNEALEAREL
                     IKVGVLKNCADDPKDIAQIIAERT"
     gene            complement(193..1503)
                     /gene="obgE"
                     /locus_tag="PLU43_05525"
     CDS             complement(193..1503)
                     /gene="obgE"
                     /locus_tag="PLU43_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOO27904.1"
                     /translation="MFADRAKIYVKSGKGGDGHVSFRREKYVPNGGPDGGDGGKGGDV
                     IFEVDEGLNTLTDFKHIRKYCAQPGENGGKRNCRGKSGGDIIIKVPAGTVLKEAESGK
                     VITDMSGDNKRIVLLKGGRGGNGNQHYATSTMQAPKYAQPGQPSQELEIAMELKVIAD
                     VGLIGFPNVGKSTFLSRVSNARPKIANYHFTTLQPYLGVVDLDDAKGFVIADIPGLIE
                     GASEGIGLGHDFLRHIERTRVMIHVVDAASAEGRDPIEDIYVINKELENYNKELAARP
                     QVIAANKTDVIIPGTDDPVARLKKEFEPQGIKVFPICAVSGQGIRELLYYVSSMLKDL
                     KDDPVIFEQEYFIGEGDSPNEPYTISYDEKEQVYFVEGPRIEKMLGYTNLDAEKGFIF
                     FQKFMRDNGIFSELESLGIQEGDTVRIYGHEFEYLREEGHYNDK"
     gene            complement(1610..1894)
                     /gene="rpmA"
                     /locus_tag="PLU43_05530"
     CDS             complement(1610..1894)
                     /gene="rpmA"
                     /locus_tag="PLU43_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOO27905.1"
                     /translation="MLKLNLQFFAHKKGMGSTKNGRDSESKRLGAKRADGQFVKAGNI
                     LYRQRGTKIHPGENVGRGGDDTLFALADGVVRFERKGRDKKQASVYPVER"
     gene            complement(1898..2218)
                     /locus_tag="PLU43_05535"
     CDS             complement(1898..2218)
                     /locus_tag="PLU43_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOO27906.1"
                     /translation="MITAKVLKKQDGSYDSFSCSGHAGFSVKGKDIVCAAVSILVINT
                     ANAMEQFTDAKFECSSESTIEWKFLSAPDAGAKLLMDSMLLGLKEIQNEYGKRYLRLI
                     FEEV"
     gene            complement(2240..2545)
                     /gene="rplU"
                     /locus_tag="PLU43_05540"
     CDS             complement(2240..2545)
                     /gene="rplU"
                     /locus_tag="PLU43_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOO27907.1"
                     /translation="MYAIIATGGKQYKVSEGDVIKVEKLGAEAGDTVTFDQVLAVSDE
                     KLSVGEDVANATVSATVVENGRGKKVIVYRYKRKSGYHKKNGHRQEYTQVKIDKINA"
BASE COUNT          668 a          711 c          447 g          844 t
ORIGIN      
        1 gtccgctcgg caataatctg tgcaatatcc ttcggatcgt ccgcgcagtt ttttaataca
       61 ccgaccttga tcagttctct cgcctcaagc gcttcattga cagaactcac aatttccggc
      121 gttaagcttg ctttccctat atgtacaaca atttccatgg tacttgcaag accttttaaa
      181 taagctctct gtttacttgt cattataatg tccttcttcc cgtaaatatt caaattcatg
      241 accgtatatt cttacagtgt caccttcctg aatacccagc gattcgagct ctgaaaagat
      301 cccgttatcc ctcatgaatt tttggaaaaa tataaaccct ttttccgcat ccagatttgt
      361 atatccaagc attttttcga tacggggccc ttcaacaaaa taaacctgct ccttttcatc
      421 ataggatatg gtatacggtt cgttcgggct gtctccttcc ccgataaaat actcctgttc
      481 aaatataacc ggatcatctt tcaggtcttt aagcatggaa cttacataat aaagcagctc
      541 cctgatgccc tgaccgctga cagcacagat cggaaatact ttgattccct gcggctcaaa
      601 ttctttcttt aaacgcgcaa cgggatcatc tgtccccgga atgatcacat ccgtcttatt
      661 tgcagctata acctgcggcc ttgccgccag ttctttattg taattttcca gttctttatt
      721 tatgacataa atgtcctcga tcggatcccg gccttccgca gatgccgcat ccacaacatg
      781 gatcatcact cttgtacgtt cgatgtgacg aagaaaatca tgcccaagtc caataccttc
      841 ggacgcgcct tcaattaatc ccggaatatc tgcaataaca aatccttttg catcatccag
      901 atcaactaca ccaagatatg gctgtaacgt tgtaaaatga taatttgcga ttttaggacg
      961 ggcattggat actcttgata aaaatgtgga tttaccgaca ttcggaaacc cgatcagtcc
     1021 gacatccgca ataaccttca gttccattgc aatttcaagc tcctggctcg gctgacccgg
     1081 ctgagcgtat tttggagcct gcatggttga agtcgcataa tgctggttac catttcctcc
     1141 cctgccgcct tttaaaagaa cgatccgctt attgtctccg gacatatccg taatgacctt
     1201 tccgctttcc gcctctttta gtaccgtgcc tgccggaacc ttgatgatga tatctcctcc
     1261 gcttttaccg cggcagtttc tttttccacc gttttcaccg ggctgcgcgc aatatttgcg
     1321 aatatgcttg aaatcagtca gggtattcag tccttcatcg acttcaaaaa taacgtcgcc
     1381 gcccttgccg ccgtctccgc cgtccggtcc gccgtttggt acatattttt cccggcggaa
     1441 ggaaacgtgc ccatctccgc ctttgccgct tttcacatat atttttgctc tgtctgcgaa
     1501 cattttcttc tcccttgata catatgcctg aaatcaatac agctgttttc aattgcatat
     1561 gaaagaacag ggttttaaac aatgaagttt aaaaccctgc gcatttatct catctctcta
     1621 ccggatatac ggaagcctgt tttttatctc ttccttttct ttcaaatctg acaacaccgt
     1681 ctgccaaggc aaataatgta tcatctcctc cgcgtcctac gttctcgccc ggatgaatct
     1741 tagtgccgcg ctgtctgtaa agaatatttc ctgcttttac aaactgtccg tcagctctct
     1801 ttgcgccaag tctttttgat tctgaatctc tgccattctt ggtagagccc attccttttt
     1861 tatgagcaaa aaattgaagg ttcagtttca acatggttta cacctcctcg aaaataagtc
     1921 tcaaatatcg ttttccatat tcattttgaa tttctttcag tcctaaaagc atggaatcca
     1981 tcaacagctt tgcaccagca tccggagcag ataaaaattt ccattcaatc gtgctttccg
     2041 aactgcattc aaatttcgcg tctgtaaact gttccattgc atttgccgta ttaatgacaa
     2101 gaatggaaac agccgcgcat acaatatcct ttcctttaac ggaaaacccc gcatgtccgg
     2161 aacacgaaaa cgaatcataa ctgccatcct gtttttttaa gacctttgct gtgatcatat
     2221 tgttaagcca cccgaccgat tatgcattga ttttatcaat tttcacctgc gtgtattctt
     2281 gtctatgacc attctttttg tgatatccgc tttttctttt gtatctgtat acaatgactt
     2341 tttttcctct gccattttct acaaccgtag ccgaaacagt tgcattcgca acgtcctcac
     2401 ctactgaaag cttctcatca ctgacagcca gaacctgatc aaatgtcaca gtatctccag
     2461 cttccgcgcc aagcttttca accttgatca cgtcaccttc ggaaacctta tactgcttcc
     2521 cgcctgttgc aataattgcg tacataaggc acctcctaaa tatttactcg ctagcttcgg
     2581 cagaacatcc aaaaactgaa agtccgttta tggcctcgct atgcggcata ctcaaacata
     2641 atagcacaga cactactgct tgtcaataaa
//
LOCUS       DAOSYE010000265         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29360_length_2663_cov_2.939417, whole genome shotgun sequence.
ACCESSION   DAOSYE010000265 DAOSYE010000000
VERSION     DAOSYE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29360_length_2663_cov_2.939417"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLU43_05545"
     CDS             <1..239
                     /locus_tag="PLU43_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOO27908.1"
                     /translation="FEQWGPFEDIEIMYDWSGKTGDFKPDLSDKEYVAALKNAMQNRV
                     DAVEGFASKKEGYDKLPDEALAALKKIVEETASL"
     gene            643..1863
                     /gene="tyrS"
                     /locus_tag="PLU43_05550"
     CDS             643..1863
                     /gene="tyrS"
                     /locus_tag="PLU43_05550"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO27909.1"
                     /translation="MTIYDELVARGLIAQVTDEAEIKDLINNGKATFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPIALLGGGTGMIGDPTGRTDMRKMLTAEDINHNIECFK
                     KQMGRFIDFGEGKAMMVNNADWLLDLNYIEVLREVGPYFSVNRMLAAECYKQRMEKGL
                     SFLEFNYMIMQSYDFLELYRRYGCNMQFGGDDQWSNMLGGTRLIRSKLGKDAYAMTIT
                     LLLNSEGKKMGKTQSGAVWLDPKKTSPYDFYQYWRNVADDDVLKCIRMMTFLPLEQID
                     EMDQWEGSQLNQAKEILAYELTTLVHGEDEAVKAQNSARALFGSKGVSEDMPVCELTE
                     ADLTDGGIDILLLLVKAELVKSKSEARRAVEQGGVMVDGEKISDIYTGFTREQLSGGI
                     VLKKGKKNFKKIKL"
     gene            1952..>2663
                     /locus_tag="PLU43_05555"
     CDS             1952..>2663
                     /locus_tag="PLU43_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO27910.1"
                     /translation="MKTNILEYLTESVSVNPKKTAYFDEKTRYTYEQLFKNTKAVGSK
                     LIRKLNVVCRPVLVYMEKTPYTLAAFFGTVASRNFYVPLDTNMPEYRIRLIVENLKPV
                     AVITDMVHFDMARSFSNTVFIFEEMIEAEIAEELLAERRKNALDADPVYVLYTSGSTG
                     VPKGVVVSHRSLIDYMEQFCNEIHIEQEDIIANQAPFYFDASLIDIYCTLKMGATMGI
                     IPLEYFSVPVKLLEFLQKY"
BASE COUNT          789 a          431 c          744 g          699 t
ORIGIN      
        1 agtttgagca gtggggacct tttgaagata tcgagatcat gtatgactgg tcaggaaaga
       61 caggagattt caagccggat ctttccgata aggaatatgt agctgcatta aagaatgcta
      121 tgcagaaccg tgtggatgca gtggaaggtt ttgcttccaa gaaggaaggt tatgataagc
      181 ttccggatga agcgcttgct gctcttaaga agatcgttga agagactgca tctttataaa
      241 aattaagttt gctgataaaa attggacacc ctgcggatct ttgcagggtg ttttcttttt
      301 gaaatgcttg acactcaaat gttttatcgt tataatgctt tcaaaaggca gtgacaggaa
      361 gaagtaacgg actggaaacc aacagaaagc agccggggga tgagaggcgc aggagagagg
      421 ttccgtgaat acatccttga gccgcgtacc gaaagggaat atagaccaag taggctgcgc
      481 cggggcgggc acacgttatc gtgccggagt attgttgttg gacagtactt gatgaggcag
      541 gctgcgtgag cagattgtga ataaaggtgg taacacggga aattgatccc gtcctttggt
      601 aaaaggacgg gatttttgtg tacaggaaaa ggagagaaga agatgacgat ttatgatgaa
      661 ctcgtggcaa gaggattaat tgcgcaggta actgatgagg cagagatcaa agatttgatc
      721 aataacggaa aggcaacgtt ttatattgga tttgacccga cggccgatag tctgcatgtc
      781 ggacatttca tggcgctgtg tttgatgaaa aggctgcaga tggcgggaaa caaaccgatc
      841 gcgctgcttg gcggaggcac agggatgatc ggggatccga ccgggcgtac ggatatgaga
      901 aaaatgctga ctgcagaaga tattaatcat aatattgagt gctttaaaaa acagatgggc
      961 cgttttattg attttggtga aggaaaagcg atgatggtca acaatgcgga ctggctgctg
     1021 gatctgaatt acatcgaagt gctgcgggag gtaggacctt acttcagtgt caatcgcatg
     1081 cttgcagcgg agtgttataa acagagaatg gaaaaaggtt taagcttcct tgagtttaat
     1141 tatatgatta tgcagagcta tgattttttg gaattatacc gtagatatgg gtgcaatatg
     1201 cagtttggcg gagacgatca gtggagcaat atgctcgggg gaacgaggct gatcagaagc
     1261 aagcttggca aggatgcgta tgcaatgacg atcacactgc ttttaaattc cgagggaaag
     1321 aagatgggaa agactcagtc cggtgctgtg tggcttgatc caaaaaagac ttccccctat
     1381 gatttctatc agtattggag aaacgtggcg gatgatgatg tgctgaaatg cattcggatg
     1441 atgacatttc tgccgctgga acagatcgat gaaatggatc aatgggaagg cagtcagctc
     1501 aatcaggcaa aagaaattct ggcatatgag ctgacaacgc ttgttcatgg agaagatgag
     1561 gctgtaaaag cacagaattc tgcaagagca ttgtttggaa gcaaaggggt ttcggaagat
     1621 atgccggtct gtgagcttac ggaagccgat ctgacggacg gagggatcga tattttgctg
     1681 ctgctcgtca aggcggaact ggttaaatcc aaatccgagg cacggcgtgc tgttgagcag
     1741 ggaggcgtta tggtagatgg agaaaaaatt tcggatattt ataccggatt tacaagggag
     1801 cagctgtctg gcggtatcgt gttaaaaaaa ggaaaaaaga acttcaaaaa aataaaactt
     1861 tgattgacgt ggcagtgtag gggtgctaca atttgacagg aagtttaata aatcgcataa
     1921 tggataagag agttgtccag gtggagccgt gatgaaaaca aatattttag aatatttaac
     1981 ggaatcggtc agtgtcaatc cgaagaagac agcatatttt gatgaaaaga caaggtatac
     2041 gtatgaacag cttttcaaga atacaaaggc ggtcggcagc aagctgataa ggaaattgaa
     2101 tgtcgtatgc cggccggtac ttgtttatat ggaaaagacg ccttatacgc ttgctgcgtt
     2161 tttcggaaca gtggcaagca ggaattttta tgtgccgctt gatacaaata tgcctgagta
     2221 cagaatacgt ctgatcgttg aaaatctgaa gccggttgct gtaattacag atatggtgca
     2281 ttttgatatg gcaagatctt tttccaatac tgtttttatt tttgaagaaa tgatcgaagc
     2341 ggaaattgcg gaagagctgt tggcggaacg caggaagaat gcacttgatg cggatcctgt
     2401 ttatgtgctt tatacatccg gttcaacagg tgtgccaaag ggcgtcgttg tcagtcaccg
     2461 ttccctgatt gattatatgg aacagttctg caatgagata catattgaac aggaggatat
     2521 cattgcaaat caggcgccgt tttattttga cgcatccctg atcgatatct actgtacgtt
     2581 gaaaatgggc gctacgatgg gaattattcc gcttgaatat ttttctgtgc cggtcaagct
     2641 gcttgaattt ttgcagaaat atc
//
LOCUS       DAOSYE010000266         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29415_length_2659_cov_3.451229, whole genome shotgun sequence.
ACCESSION   DAOSYE010000266 DAOSYE010000000
VERSION     DAOSYE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29415_length_2659_cov_3.451229"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..684
                     /locus_tag="PLU43_05560"
     CDS             100..684
                     /locus_tag="PLU43_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO27911.1"
                     /translation="MEQAECAAKIRPLLCTMDECGDCVSMMESGNTVYIALLDVLGHG
                     KKAREVAILAQGYIARSYPNALLDMMQGLHQCLHGTRGAVGVLCEIDRLTGRAVLTGV
                     GNVTARIFGRRNYRFVFGDGIIGYGEIHPVLQETELGKGDVFVLHSDGIREHFDELEC
                     IGLFKESAKVIAGGLVDRFGRESDDASCLVYKQQ"
     gene            626..>2659
                     /locus_tag="PLU43_05565"
     CDS             626..>2659
                     /locus_tag="PLU43_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO27912.1"
                     /translation="MIGLEERVTMLPVWYISSSNCVSEVVFMYEIGYLSVTDSNSVIV
                     CREKIRAALIMMEFSVISVTRMEAALSDFLRKVLDDKPILGMCVSNDSTGQTAVISVD
                     IRKQSVDFTVVKKVFEKVLVMDLSEGTVRILFEQCLRHLPAALPSSLLENIKTEFNAK
                     TREELLKEIEEKNRELTAAKEAAEEAARIKSDFLANMSHEIRTPMNAIMGMTYLIRKT
                     ELTAKQQDYVNKIHKSGQHLLGVINDILDFSKIEAGKLDIEYAEFSLKSVLENLSDLI
                     GDKCMEKGLELVFDVDPEITDCLYGDQLRISQILINYVNNAIKFTEKGEIIVRIRLTG
                     KENNRFCFRFEVQDTGIGLTPEQKDRLFQSFQQADTSTTRKYGGTGLGLAISRSLAGL
                     MGGETGVESEYGKGSTFWFTVSVKQCKRSKRLHAADVKLANRRVLVVDDNMQARNILA
                     EMLSSMSLRVDRAEDGESAVEMTKEACRADDAYEVIFMDMQMPGMSGIEAYTEILFVT
                     GEMRPHCIMVTGHGREEVFYKDGRAGFELVLVKPVNPMILFESIVSVLGGHAEEDTDN
                     TKPEKNGLSMPAGQITGVRILLVEDNELNQQVARELLEESGFLVDIAENGKAAVERIR
                     NYSYDIVLMDMQMPVMDGLEATRRIRADRKYKDLPIIARTANAMEKDRFLCREA"
BASE COUNT          789 a          447 c          765 g          658 t
ORIGIN      
        1 gacagggcct gcctgcagta agaagaatta tggatgaatt ttatattgaa tctattttag
       61 ggagagggac caggatttta acgagaaagt gggaaaagga tggaacaagc ggaatgtgcg
      121 gcaaaaatac gtcctcttct ctgtacgatg gatgagtgcg gtgactgtgt gagtatgatg
      181 gaatctggta atacggttta tattgccctg ctcgatgttc ttggacacgg aaagaaagca
      241 agggaggtcg caatactggc gcaggggtat attgcccggt cttatccgaa cgctctttta
      301 gatatgatgc agggtcttca tcagtgtctg catggaacga gaggggccgt aggggtgctg
      361 tgtgaaatcg acagattgac aggcagggcg gtgctgaccg gtgtcggaaa tgtgacggca
      421 aggatttttg gacgacgaaa ttaccggttt gttttcgggg acggaattat tggctatgga
      481 gagatccatc ctgtccttca ggaaacagaa cttggaaagg gagatgtttt tgtactgcat
      541 tcggatggta tcagagagca ttttgacgaa ctggaatgta tcggactttt taaagagagc
      601 gcaaaggtga ttgcaggagg actcgttgat cggtttggaa gagagagtga cgatgcttcc
      661 tgtctggtat ataagcagca gtaactgcgt gagtgaggtc gtgttcatgt atgagatagg
      721 gtatctgagt gtgacagaca gtaattccgt aatcgtctgc agggagaaga tccgtgcggc
      781 attaatcatg atggaatttt cagtgatttc agtcacacgg atggaagctg ctctttcaga
      841 ttttttgaga aaagttttgg atgataagcc gattctgggc atgtgcgttt caaatgacag
      901 caccggacag acggcagtta tcagtgttga tataagaaaa cagagcgttg actttactgt
      961 tgtaaaaaaa gtatttgaga aagtgctggt gatggatctt tcggaaggga ctgtccgcat
     1021 tctgtttgag cagtgtttac ggcatttgcc cgcagcgctg ccttcgtctt tgctggaaaa
     1081 tatcaaaacg gaattcaatg caaaaaccag agaagaattg ctgaaagaga tcgaagagaa
     1141 aaacagggaa ctgacagcgg caaaagaagc agcggaagaa gcggccagga taaaatcaga
     1201 ttttcttgcg aatatgagcc atgagatcag aactccaatg aatgccatta tgggcatgac
     1261 atacctgatc aggaaaacag aactgactgc aaaacagcag gattacgtca acaaaatcca
     1321 taagtccggg cagcatctgc ttggcgtcat taatgatatt ctggattttt caaaaataga
     1381 agctggaaaa ctggatatag aatatgcgga attcagtctg aagagtgtat tggagaatct
     1441 ttccgatctg atcggtgaca aatgtatgga aaaaggactg gagctggttt ttgatgtcga
     1501 tcctgaaatt accgactgtc tgtatggcga tcagcttagg atttctcaga ttttgatcaa
     1561 ctatgtcaat aatgcgatca aatttacgga aaaaggagag atcatcgtaa gaatcaggtt
     1621 gacaggaaaa gaaaacaatc gattctgttt tcggtttgaa gtgcaggata ccggtatcgg
     1681 tttgacaccg gaacaaaaag acagactttt tcaatccttt cagcaggcag atacttctac
     1741 gacaagaaaa tacggcggca cgggactcgg cctggcaata tcaagaagtc tggccggtct
     1801 tatgggagga gaaaccggtg tagagtcgga atatggaaag ggaagtactt tctggtttac
     1861 tgtatcggta aaacagtgta aaagaagtaa acggctgcat gctgcggatg ttaagctcgc
     1921 aaaccgccgt gtccttgtag tggatgataa tatgcaggca cgaaatattt tagcagaaat
     1981 gctttcttcc atgtcattgc gggttgacag agccgaagac ggagaaagcg cagttgaaat
     2041 gacaaaagag gcctgcaggg cagatgatgc atatgaagtg atctttatgg atatgcagat
     2101 gccgggaatg tcagggatcg aagcctatac ggagatactt tttgtgacag gagagatgcg
     2161 gccgcattgc atcatggtta ccgggcacgg cagggaagaa gtcttttata aagacggcag
     2221 ggcagggttt gagttggttc ttgtaaaacc ggtcaatccg atgatcttat ttgaatcaat
     2281 tgtcagtgtg ctcggcgggc atgcagagga agacacggac aatacgaaac cggaaaagaa
     2341 tggtttgtcc atgccggccg gtcagattac aggagtaagg attctgcttg tggaggacaa
     2401 tgagctgaat cagcaggttg caagagagct gctggaagag agcggatttt tggttgatat
     2461 agcggaaaac gggaaagcgg cagtagaacg cataagaaac tattcctatg acattgtgct
     2521 tatggatatg cagatgccgg tcatggacgg actggaagca acgaggcgta tcagggcaga
     2581 caggaagtat aaagatctgc cgatcatagc aaggacggca aacgcaatgg aaaaggaccg
     2641 ttttttgtgc cgggaggcc
//
LOCUS       DAOSYE010000267         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29576_length_2651_cov_2.865562, whole genome shotgun sequence.
ACCESSION   DAOSYE010000267 DAOSYE010000000
VERSION     DAOSYE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29576_length_2651_cov_2.865562"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLU43_05570"
     CDS             <1..398
                     /locus_tag="PLU43_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27913.1"
                     /translation="REMKYAKTQMRLAVFTSFISLVIIIIVAAGMAYIVPRMTILADQ
                     ADTIMQQTDEMMVQAQTVMDNLEQVTNELSESDISGMLEDVDSLVVSSEENMAAAAQK
                     IMDIDIDSLNQAIEDLGSVVSPLAKLFGR"
     gene            437..2053
                     /gene="ilvB"
                     /locus_tag="PLU43_05575"
     CDS             437..2053
                     /gene="ilvB"
                     /locus_tag="PLU43_05575"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOO27914.1"
                     /translation="MIGADAMVKCLELEGVKTIFGYPGVAICPFYNSILSTDIENILV
                     RTEQSAAHAANGYARVTGEVGVCVATSGPGATNLITGIATAYADSIPLICITGQVDSE
                     LLGSDVFQEADITGAAESFVKYSYLNKNAEDIPRIFKEAFHIASTGRKGPVLIDVPVD
                     IQNTQIMKFKYPEEVNLRTYKPTVKGHAVQIKKVIRELSKSERPIICAGGGVILSNAK
                     KELQEFSEKYQIPVVSTMMGIGVMPTNAPLYFGMVGNNGKAYANRAMNESDLLIMIGA
                     RVADRAVSQPDLITGNKILVHIDVDPAEIGKNVGPTIPLVGDIKHIFADFAKQEFSCD
                     FSNWIETLNGYKQQTPKRKPSVGNCVDPTVFIRMLSEKLDDDAVYVADVGQNQMWSCG
                     YHIMKNGQFLTSGGMGTMGYSIPAAMGAKKACPDRQVIAVCGDGAFQMSMMELATMQQ
                     HHISFKIIILNNGYLGLVREYQHNVYKGNCSAVDLSGSPDYGKLAAAYDIPYLKLDNM
                     DHAEKTIEDFLNTKEPILLECVVDPMDVAK"
     gene            2065..2559
                     /gene="ilvN"
                     /locus_tag="PLU43_05580"
     CDS             2065..2559
                     /gene="ilvN"
                     /locus_tag="PLU43_05580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOO27915.1"
                     /translation="MKRLYSVLVENRSGVLMKVAGLFSRRCFNIDSLAVGETDDPKVS
                     CITIVSSGDQRTLEQIEKQLNKKIDVIKIKTFSEEESVSRELMLVKVKYNKSNRRELM
                     EICEILRAQIVDMSKNLMTIQLCDTPEKTALLIKMLASISVVEVARTGTLALQKCALS
                     DVNN"
BASE COUNT          792 a          456 c          704 g          699 t
ORIGIN      
        1 aaagagaaat gaagtatgca aagacgcaga tgcgtctggc agtatttacg tcattcattt
       61 ccctggtcat tattattatc gtggcggccg gtatggccta tattgttccg cgaatgacca
      121 tactggcgga tcaggcggat acgatcatgc agcagacaga cgagatgatg gtgcaggcgc
      181 agactgttat ggataatctg gaacaggtaa caaacgagct ttcggaatcc gatatctccg
      241 gaatgcttga ggatgttgat tctcttgtcg tctcaagtga ggagaatatg gcggcggccg
      301 cacagaaaat catggatatt gatatagatt cgctgaatca ggcaattgaa gatctgggtt
      361 ctgtcgtgtc accgcttgcg aaactgtttg gcaggtgata aaataaaagg attgtggaaa
      421 gaaggggttg aataaaatga taggtgctga tgcaatggta aaatgccttg aactggaagg
      481 cgttaagacg atatttggtt acccgggtgt agcgatttgt cctttttata atagtatatt
      541 atctacagat attgaaaata ttcttgtgcg tacggaacaa agtgcggcgc atgctgcgaa
      601 cggttacgca agagtgacag gagaggtcgg tgtctgtgtg gcaacctcag gtccgggtgc
      661 gacgaatctg attacgggca ttgcaactgc ttatgcagac agtattccgc ttatttgtat
      721 taccgggcag gtggactcag aactgcttgg cagcgatgtg tttcaggagg ctgatattac
      781 gggggcagca gagtcttttg taaagtacag ttatttgaac aaaaatgctg aggatattcc
      841 gagaatattt aaggaagcgt ttcatatcgc gtctacagga aggaaaggtc cggttttgat
      901 cgatgtaccg gtcgatattc agaatacgca gatcatgaaa tttaaatatc cggaggaagt
      961 taatctgcgt acttataaac ctacggtcaa agggcatgcg gttcagataa aaaaagttat
     1021 cagggagctt tccaaatcag aaagaccgat catctgcgca ggcggcggag tgattttaag
     1081 caatgcgaaa aaagaattac aggaattctc tgaaaagtat cagattccgg tggtttctac
     1141 tatgatggga attggtgtga tgccaacaaa tgcgccgttg tattttggaa tggtcggcaa
     1201 caatggaaaa gcatatgcaa accgtgcgat gaatgaatcg gatctgctga tcatgatcgg
     1261 tgcgcgcgtg gcagatcgtg cggtatcgca gccggatctg atcacaggga acaaaatact
     1321 tgttcatatt gatgtggatc ctgcggaaat aggaaagaat gtcggcccga cgattcctct
     1381 tgtcggtgat atcaagcata tttttgcgga ttttgcaaag caggagtttt cctgtgattt
     1441 ttcaaactgg atcgaaaccc tgaatggata taagcagcag acgccgaaaa gaaagccgag
     1501 tgtcggaaat tgtgtggatc caacggtgtt tattcgtatg ctttccgaaa aactggacga
     1561 cgatgcggtt tatgttgccg atgtcggaca aaatcagatg tggtcatgcg gctatcatat
     1621 tatgaaaaac ggacagtttc ttacttcagg gggaatgggt acgatgggat attcgattcc
     1681 ggctgccatg ggtgcaaaga aagcatgccc ggacagacag gtcatcgctg tctgcggaga
     1741 cggagcgttc caaatgtcaa tgatggagct tgcgacaatg cagcagcatc acatttcatt
     1801 taagatcatt attttaaata acggatatct gggacttgta agggaatatc agcataatgt
     1861 atataaagga aactgttcgg cagtggacct gagcggaagt ccggattatg gaaagcttgc
     1921 ggcagcctat gacattccgt atttaaaact ggacaatatg gatcatgcag aaaagacgat
     1981 cgaagatttt ctgaatacga aagagcccat attgctggag tgcgtagtgg atccaatgga
     2041 tgttgcaaaa taggaggaaa taaaatgaaa cgattatatt ccgttcttgt ggaaaaccgt
     2101 tccggtgtat tgatgaaggt agcgggtctt ttttcaagaa gatgttttaa tattgatagt
     2161 cttgccgttg gtgagactga tgatccgaag gtttcctgca tcacgatcgt gtcatctggt
     2221 gatcagagga cattggaaca gatcgaaaag cagctgaata aaaagatcga tgtaatcaag
     2281 atcaagacgt tttccgaaga agagagtgtc agcagggaat tgatgcttgt caaagtaaag
     2341 tacaacaaaa gcaacagacg ggagctgatg gagatctgtg agatattaag agcgcagatc
     2401 gtagatatgt caaaaaatct aatgacgatt cagctttgcg acacaccgga aaaaactgcc
     2461 cttctgatta agatgctggc ttcgatcagt gttgtcgaag tggctcgtac cgggaccctg
     2521 gctcttcaga aatgtgcttt aagtgatgtg aataattaaa ggtcaggcat gacttcgcat
     2581 tattaaaata tcaaattaga agagctttcc ataacgtaag gttataacaa gcatgaatat
     2641 gatacataca t
//
LOCUS       DAOSYE010000268         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29753_length_2641_cov_3.832947, whole genome shotgun sequence.
ACCESSION   DAOSYE010000268 DAOSYE010000000
VERSION     DAOSYE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29753_length_2641_cov_3.832947"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1346
                     /locus_tag="PLU43_05585"
     CDS             150..1346
                     /locus_tag="PLU43_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO27916.1"
                     /translation="MKKKLLSILLSTAMTAALLAGCGSSAATEEAAATEEAATEETAT
                     EEEAAPAEDTADETESTETASEPSEDGYELALVTDLGTIDDKSFNQGAWEGLKQYAEE
                     NSISYKYYQPQEGTTDSYVETIGLAVEGGAKLVVCPGYLFEEPVYIVQDEYPDVHFIL
                     LDGEPHNADYSEYKTGDNVMPILFQEDEAGFLAGYAAVKDGYTKLGFMGGMAVPAVIR
                     YGYGYVQGADTAAAEDGVDVEIMYNYTGAFAATPEAQAMAASWYQNGTEVIFGCGGAV
                     GNSVMAAAEEAGTKVIGVDVDQSSESDTVITSAMKQLSVSVYDGIKAYYDGTFPGGET
                     SVFSAANDGVGLPMESSKFENFTQDDYDALYEKLVNGDFSINNSTEDSTTADLTLTNT
                     TVTFVE"
     gene            1502..>2641
                     /locus_tag="PLU43_05590"
     CDS             1502..>2641
                     /locus_tag="PLU43_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27917.1"
                     /translation="MDYIIEMLHITKEFPGIIANDDITLQVKKGEIHALLGENGAGKS
                     TLMSVLFGLYQPEHGIIKINGEEKKISGPLDANALGIGMVHQHFKLVHNFTVLQNIVL
                     GVETVSTGFLKMDEARKKVVELSERYGLYVDPDALIEDITVGMQQRVEILKMLYRENE
                     ILIFDEPTAVLTPQEIDELMEIMKGLVKENKSILFITHKLNEIKAVADRCTVLRRGKY
                     IGTVNVADTTKEQMSEMMVGRKVNLSIEKKPAEPKEVALEVKNLCVRSHHEGHTKDVV
                     SNVSFQVHKGEIVCIAGIDGNGQTELVHAITGLGETASGSIEINGEDVTKKSIRYRNT
                     HGMSHIPEDRHRHGLILDYNLAYNLVLKQYFTKDFQKGGFLKKDKI"
BASE COUNT          817 a          546 c          630 g          648 t
ORIGIN      
        1 tatcattttt gaaaaacaat tggataattg ccaacgttta cttgacacaa acaaattttt
       61 ggttacgatt gaaaataaat atggcatatg ctatactgat aaaaatatat gaattatcat
      121 atataaatat aattagggag gacttattta tgaaaaagaa attattaagt atcttgctta
      181 gtaccgcaat gacagccgct ttacttgctg gatgcggcag cagcgcagct acggaagaag
      241 ccgcagcgac agaggaagct gcgacagagg aaactgcaac agaggaagaa gccgctccgg
      301 ctgaggatac ggcagatgag accgaatcca cagaaaccgc ttccgaacct tcagaagacg
      361 gttatgaact ggcacttgta actgaccttg gcacgatcga cgacaaatct ttcaaccagg
      421 gtgcatggga aggtctgaag cagtacgcag aagaaaacag catttcctac aaatactatc
      481 agcctcagga aggcacgaca gattcttatg tggaaacgat cggccttgca gtggaaggcg
      541 gagcaaaatt agttgtttgt cccggctact tatttgaaga acccgtatac atcgttcagg
      601 atgaatatcc ggacgtacat ttcattctgc ttgacggcga gcctcacaat gccgactaca
      661 gcgaatacaa aacaggcgac aatgtaatgc ctattctgtt ccaggaagat gaagcaggtt
      721 tccttgcagg ttatgcagca gtaaaggacg gctacacaaa gcttggtttc atgggcggta
      781 tggcagttcc ggctgtgatc cgttacggtt acggctatgt acagggtgca gatactgcag
      841 ctgctgaaga cggtgttgac gtagagatca tgtacaacta taccggtgca tttgcagcta
      901 ctcctgaagc acaggctatg gcagcaagct ggtatcagaa cggcacggaa gtaatctttg
      961 gctgcggtgg cgctgtcggc aactccgtaa tggctgctgc tgaagaagca ggtacaaagg
     1021 tcattggtgt tgacgtagac cagtcttccg aatcagatac ggttatcaca tccgctatga
     1081 aacagctgag cgtttccgta tacgacggta tcaaggctta ttatgacggt acattcccag
     1141 gcggagaaac atccgtattc tcagctgcaa atgacggcgt aggccttccg atggaatctt
     1201 ccaagtttga gaacttcaca caggatgatt atgatgcatt atatgaaaag cttgtaaacg
     1261 gtgatttctc aatcaacaat tccacagagg actctacaac ggcagatctt acattaacaa
     1321 atacaacggt tacttttgtt gaatagtttt cattgaaaac tcattaagca aaaaagatgc
     1381 cgatgtcgtt tttagacggc gtcggcatct tttttaaagt ttatttcaca ggaaagcagt
     1441 caaccgccct tctgttgact gttttcctgc aaaataaatg ataaaggaga tggagaagct
     1501 tatggattac attatcgaga tgctgcatat cacgaaagaa tttcccggca tcatcgcaaa
     1561 tgacgacatt accctgcaag taaaaaaggg ggagatccat gccctgcttg gtgaaaacgg
     1621 cgccggaaaa tccacgttga tgagtgtgct tttcggactt taccagcctg aacatgggat
     1681 aatcaaaatc aacggcgaag aaaaaaaaat cagcggacca ctggatgcca atgcactggg
     1741 gatcggtatg gtgcatcagc actttaagct tgtacataat ttcactgtcc tgcagaatat
     1801 cgtattaggc gtggaaacag tcagcacagg atttttaaag atggacgagg cacgtaagaa
     1861 agtcgtagaa ctgtccgaac gctatggtct ttacgttgat ccggatgccc tgatcgagga
     1921 tattacagtc ggcatgcagc agcgtgtgga aattctcaaa atgctttacc gtgaaaatga
     1981 aatactgatt tttgatgagc cgactgcagt tctgactcca caggagatcg atgaattgat
     2041 ggagatcatg aaagggcttg tcaaagaaaa caaatccatt ctctttatta cacataagct
     2101 taatgaaatc aaggctgttg cagatcgctg cacggttctt cggcgcggaa aatatattgg
     2161 tactgtcaat gttgcggaca cgacaaagga acagatgtcc gaaatgatgg ttggccgcaa
     2221 ggtcaatctc tccatcgaaa aaaagccggc agaaccaaaa gaagttgctc ttgaagtgaa
     2281 aaatctctgt gtaagatctc atcatgaagg gcatacgaag gacgttgtct caaatgtcag
     2341 ttttcaggta cataaaggtg agatcgtctg tattgccgga atcgacggaa atgggcagac
     2401 agagctcgta catgcaatta cagggcttgg tgaaactgct tccggatcga tcgagatcaa
     2461 cggcgaagac gtcaccaaaa aatccatccg ctacagaaat acgcacggca tgtcccacat
     2521 cccggaagac cggcacagac acggtctgat ccttgactac aatcttgctt acaatcttgt
     2581 cctgaaacaa tactttacaa aggactttca aaaaggcggg tttctgaaaa aagataagat
     2641 t
//
LOCUS       DAOSYE010000269         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_29828_length_2637_cov_2.582881, whole genome shotgun sequence.
ACCESSION   DAOSYE010000269 DAOSYE010000000
VERSION     DAOSYE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29828_length_2637_cov_2.582881"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLU43_05595"
     CDS             complement(<1..844)
                     /locus_tag="PLU43_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO27918.1"
                     /translation="MGVLNKKVVFDNIETVVENTLGGIGVFEVRGEKITALFLNDGFY
                     RMLGYSKKECAVFLKNIEANIIREDLAVFRQGITDILKDDGWCEIEFRTVTKDGGIRW
                     LQVRGNLFSRTEEYSIVNAIILDATERKNIQTELAEQAERLHLLSESEGEMIFDYNAK
                     TDVLVIKFAKSIGGNDILIKDYVQKDSHVCIDEKDRKKFLEIIAALLKKPMKDAIDIR
                     AKIHGDANRWYRINFASILGNEGYVTRAVGRITDIHEKKLEELKIEEKAERDPLTGLY
                     NKGTT"
     gene            complement(887..1558)
                     /locus_tag="PLU43_05600"
     CDS             complement(887..1558)
                     /locus_tag="PLU43_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOO27919.1"
                     /translation="MEQYKIDHLYDLKESIAGAFLSGFSYPWEALPKIGECIIEIGNT
                     LNEEDYEKRGENIWIAKSAKVAPTAYINGPAIIGKEAEIRHCAFIRGNAIVGEGAVVG
                     NSTELKNVILFNRVQVPHYNYVGDSILGFRAHMGAGSITSNVKADKTLVEVKNGMERL
                     ETGLKKFGAMLGDYVEVGCNTVLNPGTVIGRHTNVYPCSMVRGCIAENSIYKKQGEIA
                     AKHMQ"
     gene            complement(1587..>2637)
                     /locus_tag="PLU43_05605"
     CDS             complement(1587..>2637)
                     /locus_tag="PLU43_05605"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOO27920.1"
                     /translation="LWAAGIQSSLLAKEIQKNLSGLRNYEKVREYSEEILGSGTYRTI
                     AKTRYVNDKQITDHYAVIPTGQGFDALRGLSGRAENVYEIIVRRFLSIFYPPAVYQKT
                     GITAGIENERFFANFKVLAEEGYLKVNQYSFIKNRQEKRGPDDTFDEHSADEEEEKKY
                     DAEFLKKLSHLKKGDILSVNQMELKEGETSPPKRYTSGSMILAMENAGQLIEDEELRE
                     QIKGNGIGTSATRAEILKKLVTNKYLALNKKTQVIMPALLGEMIYDVVDTSIRPLLDP
                     KLTASWEKGLTQVSEGTITSKLYMSKLNDYVARRINAVKGLNNQTALSGMFVKAAGNY
                     KKSPSSAKTASKRNS"
BASE COUNT          635 a          693 c          442 g          867 t
ORIGIN      
        1 gtgtggttcc tttattatag agtccggtaa gcggatccct ttccgctttt tcctcgatct
       61 tcaactcctc cagctttttt tcatgaatat ccgtaattct gcctacggct cttgtaacat
      121 atccttcatt tccaaggata ctggcaaaat tgatccgata ccatcggttt gcatctccat
      181 gtatttttgc cctgatatcg attgcatcct tcattggctt tttcagcagc gccgctatga
      241 tctccaaaaa tttctttctg tctttttcat caatgcacac atgcgaatcc ttctgcacgt
      301 aatccttgat cagaatatca tttccgccga tactctttgc aaacttgata accaaaacat
      361 ctgtttttgc attatagtca aaaatcattt caccttccga ttcagataaa agatgaagcc
      421 tctccgcctg ttccgcaagc tctgtctgta tattttttct ttctgtcgca tccagaataa
      481 tagcattgac gatactgtac tcctctgtgc gtgaaaacag atttcctctt acctgaagcc
      541 agcggatccc gccgtctttc gtaacagtcc gaaactcgat ctcacaccac ccgtcatctt
      601 ttaaaatatc cgtgattccc tgcctgaata cggccagatc ctcgcggata atattagcct
      661 cgatattttt caggaatacg gcgcattctt tttttgaata tccgagcatc cggtaaaacc
      721 cgtcattcag gaaaagagca gtgatcttct ctcccctgac ctcaaaaacg ccaattccgc
      781 caagcgtatt ttcaacaact gtttcaatat tgtcaaatac aacttttttg ttcagcactc
      841 ccatctatgc cgctcctttc acaaactgca tccatttgtg acttcattac tgcatatgtt
      901 ttgcagcaat ctctccctgt tttttataaa tagaattttc cgctatgcag ccgcggacca
      961 tggaacaggg ataaacattc gtatgccttc caatgacggt ccctggattc agcaccgtat
     1021 tacagcctac ctccacataa tccccaagca ttgcaccaaa tttcttcaag ccggtttcca
     1081 gtctctccat tccgttttta acttccacaa gtgtcttatc ggcctttaca tttgaagtaa
     1141 tggaaccagc tcccatgtgt gccctgaatc cgagaatgga atctcccaca taattgtaat
     1201 gcggaacctg caccctgtta aataaaatca cgttttttaa ttccgtcgaa tttccgacga
     1261 ccgctccctc accaacaatt gcatttcccc tgataaacgc acagtgtctg atttccgcct
     1321 cttttccaat aatggccggt ccattgatat atgcagttgg tgccactttt gcgctttttg
     1381 ctatccagat attctctccc cgtttttcat aatcttcttc atttaatgta tttccaatct
     1441 caataataca ttccccgatt ttcggaagtg cttcccacgg ataggaaaac ccggaaagaa
     1501 atgcacctgc gattgactcc tttaaatcat ataaatgatc gatcttgtat tgttccatag
     1561 gattacctgc tttctgttat tcataatcaa ctgtttcttt ttgaagcggt ctttgcggac
     1621 gacggcgatt ttttgtaatt tcccgctgct tttacaaaca tgccgcttaa cgcagtttga
     1681 ttattcagtc cttttaccgc attgatccgc cttgccacat aatcgttcag cttcgacata
     1741 taaagctttg atgtgatcgt tccttcggac acctgtgtca gacccttttc ccagctggct
     1801 gtcagcttag ggtcaagcag cggccgtatg gaagtgtcca caacatcata gatcatttct
     1861 ccaagaaggg ccggcataat gacctgtgtt tttttattaa gagccagata cttatttgtg
     1921 acaagcttct taagtatttc cgccctggta gccgatgtcc cgattccgtt ccccttgatc
     1981 tgttcccgca gttcctcgtc ctcgatcagc tgcccggcat tttccattgc aaggatcatg
     2041 gaaccggaag tatacctctt tggcggagat gtttcccctt cttttaattc catctgattg
     2101 acagaaagta tatcgccttt tttcaaatgg ctcagttttt tcagaaattc agcatcgtat
     2161 tttttctctt cctcttcatc cgcagaatgt tcgtcaaatg tatcatcagg gccgcgcttt
     2221 tcctgcctgt tttttatgaa cgagtattgg ttcactttta aatatccttc ttctgcaagc
     2281 accttaaaat tagcaaaaaa tcgttcattt tcaattcctg ccgtaattcc tgtcttctgg
     2341 taaactgccg gcggatagaa aatagaaaga aagcgcctga cgattatttc atatacattt
     2401 tcagcccggc cagaaaggcc ccttaacgcg tcaaacccct gtcctgtcgg aatgactgca
     2461 taatgatccg tgatctgctt gtcattgaca taacgggtct ttgcgatcgt ccgatacgtt
     2521 ccgctcccca atatttcttc agaatattcc ctgacctttt cataattgcg cagacccgaa
     2581 agattttttt gaatttcctt cgccaataga gaagattgaa tacccgccgc ccatagt
//
LOCUS       DAOSYE010000270         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30021_length_2626_cov_2.535978, whole genome shotgun sequence.
ACCESSION   DAOSYE010000270 DAOSYE010000000
VERSION     DAOSYE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30021_length_2626_cov_2.535978"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLU43_05610"
     CDS             <1..958
                     /locus_tag="PLU43_05610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOO27921.1"
                     /translation="YVLEKADRMPIHILAAATVTKGLQGKILTDMQALHAAGAAGFTD
                     DGIPIMDVKLLKEAFLKAKELNVPVSLHEEDPSLIVNNGVNAGSAAEKLGIGGSRREA
                     EISIVKRDLQIALETGADVNIQHVSARETVELIREAKRLTARIHAEATPHHFTLTEDA
                     VLKYGTLAKMNPPLRTEEDRLAIIEGLKDGAIEMIATDHAPHSMEEKKKPLTEAPSGI
                     IGLETALSLGITELVKKGYLTLPELIERMTSGPAKLYHLPAGGLEEGTAADITLFDEN
                     EKWTVEQFLSKACNSPFVGMELTGKVKYTICRGKLVYSDGHV"
     gene            980..1738
                     /locus_tag="PLU43_05615"
     CDS             980..1738
                     /locus_tag="PLU43_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOO27922.1"
                     /translation="MGEKKKEQAVVLSQEKIAEGIYSLWIKTNMAENAKPGQFISIYT
                     KSDSRLLPRPISICEIKKDALRIVYRIAGKGTEEFASYRTGDEIDILGPLGNGFPINA
                     EPAFLIGGGIGIPPMLALAKELQCEKNIVLGYRDELFLNEELAEYGNVTIATEDGSAG
                     TKGNVFDAVRENGLSAKVIYACGPTPMLRAVKAYALEHDIEAWVSMEERMACGIGACL
                     GCVCRTKEKDEHSQVNNKRICKEGPVFSVREVEL"
     gene            1738..>2626
                     /locus_tag="PLU43_05620"
     CDS             1738..>2626
                     /locus_tag="PLU43_05620"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOO27923.1"
                     /translation="MNTKVTIAGVTFKNPIMTASGTFGSGMEYGEFVDLNKLGAVITK
                     GVSNVPWEGNPTPRIAETYGGMINAIGLQNPGIEVFIKRDLEFLKQYDTKVIVNVCGK
                     SVEDYVEVVERLSDEAVDLLEINVSCPNVKEGALAFGQSCPALFHITEEIKKKAKQPV
                     IMKLSPNVTDITELAKTAEAAGADAVSMINTLTAMKIDIHKRSFALANQTGGLSGPAI
                     KPVAIRMVYQTAQAVKIPIIGMGGVTNAEDAIEFMMAGATAVAVGAMNFHNPYTTVEV
                     IDGIENYMNRYHLTDIKEII"
BASE COUNT          796 a          524 c          740 g          566 t
ORIGIN      
        1 gtacgttctg gagaaagcgg acaggatgcc gatccatata ctggcagcag ccactgtgac
       61 aaaaggcctg caggggaaaa tactgacaga catgcaggca ctgcatgcgg ccggggcggc
      121 gggattcacc gacgacggaa ttccgatcat ggatgtgaag ctgctgaaag aggctttttt
      181 aaaggcaaag gaattaaatg taccggtcag tctgcacgaa gaagatccga gcctgatcgt
      241 caataacgga gtcaatgccg gaagcgcggc agaaaagctt ggaatcggcg gttcacggcg
      301 ggaagcggaa atatcgattg tgaaacggga tctgcagatc gcgcttgaaa cgggtgcgga
      361 tgtcaatatc cagcatgtca gtgcaaggga gactgttgaa ctgatcaggg aagcgaaacg
      421 ccttacggca cgtatccatg cggaagcaac accgcatcat tttaccctga cagaggacgc
      481 cgtattaaag tatgggacac tggccaaaat gaatccgccg cttcggacag aggaagaccg
      541 gcttgcaatt atagaaggac taaaggacgg cgcgatcgaa atgatcgcaa ccgatcatgc
      601 gcctcacagc atggaagaaa agaaaaagcc gctgacagag gcaccaagcg gtattatcgg
      661 cctggagacg gcgctgtcgc tcgggattac ggagcttgtg aaaaaagggt atctgacact
      721 gccggaactg atcgaacgta tgacatccgg accggcgaag ctttatcatt tacctgcagg
      781 cggtcttgaa gaagggacag cagctgatat tacgctgttt gatgaaaatg aaaaatggac
      841 agtggaacag tttttgtcta aagcctgtaa ttcgccgttt gttggcatgg aattgacggg
      901 taaagtgaaa tatacaattt gccgggggaa gcttgtgtat tccgacgggc atgtgtaaaa
      961 tcaatgaagg gaacagatca tgggagaaaa aaagaaagaa caggcagtcg tattatcgca
     1021 ggagaagatc gcggagggta tttacagcct gtggataaaa accaatatgg cagaaaatgc
     1081 aaagccggga caatttattt ccatttatac gaaaagtgac agcagactgc tgccgaggcc
     1141 gatcagtata tgtgaaatca aaaaggatgc acttcggatc gtatacagga tcgcaggaaa
     1201 aggaacggaa gaatttgcat cttaccggac aggagatgag atcgacatac tgggaccgct
     1261 tggaaacgga tttcccataa acgctgaacc ggcattttta attggaggcg gaatcggcat
     1321 tccgccgatg cttgcgcttg caaaagagct gcaatgcgaa aaaaatattg ttttgggcta
     1381 cagagacgaa ttgtttctaa atgaagagct tgcagaatac ggaaacgtaa cgatcgcaac
     1441 agaagacggc agcgcgggaa caaaaggaaa tgtatttgac gccgtcaggg aaaacgggct
     1501 gtctgcaaag gtcatttatg catgcggtcc gacaccgatg ctgcgtgccg tgaaagcgta
     1561 tgcactggag catgacatag aggcatgggt ctccatggaa gagcgaatgg cctgcgggat
     1621 cggagcctgt cttggctgtg tgtgcaggac aaaggaaaaa gatgagcatt cccaggtgaa
     1681 caataaacga atatgcaagg aaggacctgt attttctgtc agggaggttg aactgtaatg
     1741 aatacgaaag taacgatcgc gggtgttaca ttcaaaaatc caattatgac tgcttcagga
     1801 acctttggtt ccggaatgga gtacggagag tttgtcgatc tgaacaaact cggagcagtc
     1861 attacaaaag gagtatccaa tgtgccgtgg gaaggaaatc cgacaccgcg catcgcagag
     1921 acgtatggcg gcatgatcaa tgcgatcggc ctgcagaatc ctggtattga agtgtttatt
     1981 aaaagggatc tggagttttt gaagcaatat gatacaaaag tgatcgttaa tgtatgcggg
     2041 aaaagtgttg aggattatgt tgaagtcgta gaaaggcttt ccgacgaagc cgtcgatctg
     2101 cttgaaatca atgtatcctg tccgaatgtc aaggaaggtg cgcttgcgtt tggacaatcc
     2161 tgccctgccc tgttccacat tacggaagaa ataaagaaaa aagcgaaaca gccggtgatc
     2221 atgaagcttt caccgaatgt tacggatatt acggagctgg ccaaaacagc cgaagcggcg
     2281 ggcgccgatg ccgtgtcaat gatcaacacc cttaccgcaa tgaagatcga cattcataaa
     2341 agaagctttg cgctggccaa tcagacaggt ggtctttccg gtccggcgat caagccggtt
     2401 gcaattcgga tggtctatca gacggcacag gctgtcaaaa ttccgattat cggtatgggc
     2461 ggtgttacaa atgcggagga tgcaattgaa tttatgatgg caggcgccac tgccgtggct
     2521 gtcggtgcaa tgaattttca caatccatat acaacggtag aagtgatcga cggaattgaa
     2581 aattatatga accgttatca ccttacagat attaaagaga ttattt
//
LOCUS       DAOSYE010000271         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30051_length_2624_cov_3.191514, whole genome shotgun sequence.
ACCESSION   DAOSYE010000271 DAOSYE010000000
VERSION     DAOSYE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30051_length_2624_cov_3.191514"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLU43_05625"
     CDS             complement(<1..552)
                     /locus_tag="PLU43_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOO27924.1"
                     /translation="MAENDNRKDEKEITAHDHKDHKDEYEDVCYLCRRPESVAGRMFK
                     LPNNISICSDCMHKTMDTMNQLDYQNMIQAAVDASKSGKNEKEDGKENEDEKNGQPPN
                     IQGIPNFPGISFLNMSDLQGMISNKQKIKKKKDPSEEEKKSVIDIKDIPQPHKIKASL
                     DEYVVGQEYAKKVISVAVYNHYKR"
     gene            complement(568..2394)
                     /gene="glmS"
                     /locus_tag="PLU43_05630"
     CDS             complement(568..2394)
                     /gene="glmS"
                     /locus_tag="PLU43_05630"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOO27925.1"
                     /translation="MCGIVGYIGKKQAAPILLDGLSKLEYRGYDSAGMAVYNGTEIKM
                     VKAMGRLKVLEEMTHGGATLPGTIGIGHTRWATHGQPSDTNSHPHFNKNGTIAVVHNG
                     IIENYMSLKNKLISKGYEFVSETDTEVLAHLIDYYYNGNPLEAITKVMHRVEGSYALG
                     IMFAEHPDQIFAVRKDNPLIVGKSEEGCFIASDVPSILKYARNVYFINNQEVVRLRAD
                     HMHFYTVDEEEIQKEAVYIDWDADAAEKGGYEHFMLKEMYEQPKTVRDTFSPRIKNNQ
                     IEIEELNISDEEIRQLSRLHIIACGSAYHAGVSGKYVMEGLARIPVEVDLASEFRYRD
                     PIIGEGALVIVISQSGETADTLAALREAHKRGAKVLGIINVVGSSIAREADSLMYTWA
                     GPEIAVATTKAYSAQLIALYLLAMKFAQVRNKISQTEFEKMLSDMQKLPEQIEMLLAN
                     KEKIQRFANRYIAAKDIFFIGRGIDYAISLEGSLKLKEISYVHSEAYAAGELKHGTIS
                     LIEDGTLVTAVLTQEDLYKKTISNMVEVKTRGAFVLAVTNEGHTDIEKTADYVLYIPL
                     TNKYFSNSLAVIPLQLFAYYVSVGKGCDVDKPRNLAKSVTVE"
     gene            complement(2437..>2624)
                     /locus_tag="PLU43_05635"
     CDS             complement(2437..>2624)
                     /locus_tag="PLU43_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO27926.1"
                     /translation="ILADLRYFKERKKGHLIFPFVILCAWRYAGYRLGKKYDRLSRRK
                     IRHCTMNALYFEKMWND"
BASE COUNT          663 a          686 c          445 g          830 t
ORIGIN      
        1 acgtttatag tgattataaa cagccacaga gatcaccttt ttcgcatact cctgcccgac
       61 aacatattcg tcaaggcttg ccttgatctt gtgcggctgc ggaatatcct taatatcgat
      121 aacagatttc ttttcttcct cagagggatc ttttttcttt tttatcttct gcttgttaga
      181 aatcattccc tgcagatcgg acatattcag aaaactgatc cccggaaaat taggaattcc
      241 ctgtatattc ggcggctgcc cattcttttc gtcttcgttt tcttttccat cttccttttc
      301 attctttccg ctttttgacg catcaactgc tgcctgaatc atattctgat agtcaagctg
      361 attcatcgta tccattgttt tatgcataca atccgaacag atcgaaatat tattcggcag
      421 cttaaacatt ctgcctgcaa cactttcggg acgtctgcac aaataacaca catcttcata
      481 ttcatcctta tgatccttat ggtcgtgcgc cgtaatttct ttttcgtctt ttctattgtc
      541 gttttccgcc ataatgtctc ctcaatttta ctccactgtg actgatttcg ccagattccg
      601 cggtttatcc acatcacagc ccttgccgac cgatacataa taggcaaaaa gctgaagcgg
      661 tatcactgca agtgaattag aaaaatattt atttgtcagt ggaatataaa gcacataatc
      721 cgctgttttt tcaatatccg tatggccttc gttcgttacc gcaagtacaa aagctcctct
      781 cgtctttacc tctaccatat tgctgatcgt ttttttatac agatcttcct gagtcagcac
      841 agctgttaca agagtcccgt cttctatcag agaaatcgtg ccatgcttca attcaccggc
      901 cgcatatgcc tccgaatgca cataggaaat ttccttaagc ttaagagacc cttctaaaga
      961 aatagcatag tcgatgcctc ttccaataaa gaaaatatcc tttgccgcaa tataacgatt
     1021 tgcaaaccgc tgtatctttt ctttattagc aagcagcatt tcgatctgct ccggcagctt
     1081 ctgcatatcg gacagcattt tctcaaactc tgtctgtgag atcttgtttc tgacctgtgc
     1141 aaacttcatg gcaagtaaat acagcgcgat cagctgtgcg gaatatgcct ttgttgttgc
     1201 aacagcaatt tccggccctg cccatgtgta catgaggctg tccgcttccc ttgcaataga
     1261 acttccgact acattaataa taccaagcac ctttgctccg cgtttatgtg cttctcttaa
     1321 ggccgcaagc gtatccgccg tctcgcccga ctggctgata acgatcacaa gcgccccttc
     1381 tccgataatc ggatcccgat atctgaattc cgaagccaga tccacctcga ccggaattct
     1441 tgcaagccct tccattacat attttccgga tactccggca tgataagcgg aaccgcatgc
     1501 tataatgtga agtctggaaa gctggcggat ctcctcatca ctgatattca gctcctcgat
     1561 ctctatttga ttattcttga tcctcggact gaacgtgtca cgtacggtct ttggctgctc
     1621 atacatttcc ttgagcataa aatgctcata accacccttt tccgccgcat cggcgtccca
     1681 gtcgatataa acggcttcct tttggatctc ctcttcgtct accgtataaa aatgcatatg
     1741 atcagcacgc agccgcacaa cttcctgatt attaataaaa tatacatttc ttgcatattt
     1801 caaaatggaa ggcacgtccg atgcaataaa gcagccttcc tcactttttc caacaatcag
     1861 cggattatct ttgcggaccg caaagatctg atcagggtgt tcggcaaaca taataccaag
     1921 cgcataggaa ccttctacac gatgcatgac cttcgtgatc gcttccagcg gattcccgtt
     1981 ataataataa tcgatcagat gtgccagcac ttccgtatcg gtttccgata caaattcata
     2041 acccttggag atcagtttgt tttttaatga catataattt tcaataatac cattatgaac
     2101 aacggcaatc gttccatttt tattaaaatg aggatgtgaa tttgtgtcgg aaggctgtcc
     2161 atgtgtcgcc catctcgtat gtccgatccc gatcgtacct ggcagtgtcg cgcctccgtg
     2221 tgtcatctct tccagcacct tcagccttcc cattgccttt accatcttga tttcggtccc
     2281 gttataaact gccattcccg ccgaatcata acccctgtat tcaagctttg acaacccgtc
     2341 aagtaaaata ggcgctgcct gctttttacc aatataacct actattccgc acatctttct
     2401 gtcctcctgt tctgtatacc tgcacaagct tactactcag tcattccaca ttttttcaaa
     2461 atacagcgca ttcatcgtac agtgccgtat tttgcggcgg gaaagacgat catatttttt
     2521 cccaagccga tatcctgcat atctccaggc acataaaatc acaaatggaa aaatcaaatg
     2581 gccttttttt ctttctttaa aataccttaa atctgccaat atat
//
LOCUS       DAOSYE010000272         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30076_length_2623_cov_2.889408, whole genome shotgun sequence.
ACCESSION   DAOSYE010000272 DAOSYE010000000
VERSION     DAOSYE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30076_length_2623_cov_2.889408"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..613
                     /locus_tag="PLU43_05640"
     CDS             230..613
                     /locus_tag="PLU43_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27927.1"
                     /translation="MAKKLGRFLAFAAVAGAAAAGVYCYLNKKDKEPCSCKDDFDDDI
                     EKFFDDEKEKAAPDRGYVSLNKSASDPAKDAIKNAVAKAADELKEKETETADGVGLVK
                     DSSSEAAEFAFKEFKNSDEDKKEEA"
     gene            complement(635..1477)
                     /locus_tag="PLU43_05645"
     CDS             complement(635..1477)
                     /locus_tag="PLU43_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOO27928.1"
                     /translation="MGILSGLGNMGLGKFENTELFEKESEKEKKHSGTAKVMTELDFL
                     FDKSYTCPVCDEEFKVRTIKVGKAKLLGTDTDLRPKYEKIDPLKYDVIVCPNCGYAAI
                     SRYYKSITPPQAKLIKENISKNFKNFHEKMEYTYDDAIERYQLALANAVVKRARASEK
                     AYICLKMAWLVRGKMETYELESPDYDEVMEQMQQDEAELLKNAMEGFVAARQTENFPL
                     CGMDEMTVDYLIAVIATKFEKYEIASKMISGILISPNANARMKDKARDLKHEILMALK
                     QKKE"
     gene            complement(1547..>2623)
                     /locus_tag="PLU43_05650"
     CDS             complement(1547..>2623)
                     /locus_tag="PLU43_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO27929.1"
                     /translation="NTGDRWCIYPMYDFAHPIEDAIEGVSHSICTLEFEDHRPLYDWV
                     VRELAYEYPPKQIEFSKLYLTNVVTGKRYIKKLVEDGVVDGWDDPRLVSIAALRRRGF
                     TPESIKMFVELCGISKSQSSVDYAMLEYCIREDLKLKKPRMMAVLEPVKLIIDNYEEG
                     RTEYLEVPNNLENEALGTRMVPFSRELYIDREDFMEEPPKKYFRLFPGNEVRLMNAYF
                     VTCTGFEKDADGMVKTVHCTYDPDTKCGSGFCGRKVKGTIHWVSAPHAIKAEVRLYEN
                     LVDEEKGVYNEEDGSMNLNENSLTVLKDCRLEQNLEDAEAYDSYQFVRKGFFCADAKD
                     SKPHAPVFNRIVSMKSSFRLPANG"
BASE COUNT          688 a          650 c          476 g          809 t
ORIGIN      
        1 accctgtttc tgttattcat ataaaaatct ccttttaata aaaatctctg tcattcctga
       61 ttcttacctt tatcatatgg catttccatt cgtattccta gttacatttg tcatgatttg
      121 gcgcgcttct ttcatgtatc tattttcata aatttctgat taatctcttt tcatttttgt
      181 aaaatagtat tacaatgata ataccattga aaatgaaagg agctaacaaa tggcaaaaaa
      241 attaggcaga tttctcgcat ttgctgctgt tgcaggcgca gctgccgcag gcgtttactg
      301 ttatctgaac aaaaaagata aggagccctg cagctgtaag gatgattttg atgacgatat
      361 agaaaaattc tttgatgatg aaaaggaaaa ggcggcaccg gacagaggtt atgtatccct
      421 gaacaaatcc gcatcagacc cggcaaaaga cgcaattaaa aacgcggttg caaaagcagc
      481 cgacgaactg aaagaaaaag aaaccgaaac agccgacggc gtcggacttg taaaggattc
      541 ttcctctgaa gcagccgaat ttgctttcaa agaatttaaa aactctgacg aggataaaaa
      601 agaagaggca taacgccttt tcttttttct gtttttattc ttttttctgc ttcagcgcca
      661 ttaagatctc atgcttcaga tcccgcgcct tatctttcat acgggcattc gcattagggg
      721 agatcaatat gcccgaaatc attttggaag caatctcata cttttcaaat ttcgttgcaa
      781 taactgcaat caaataatcg actgtcatct catccatgcc gcacagcgga aaattctctg
      841 tctgtcttgc ggcaacgaac ccttccattg cattttttaa aagctcggct tcatcctgct
      901 gcatctgctc catgacttcg tcataatccg gcgattccag ctcataggtt tccattttgc
      961 cgcggacaag ccatgccatt ttcaggcata tatatgcttt ttcgcttgcc cttgcccgtt
     1021 ttacgaccgc atttgcaagc gcaagctgat agcgttcgat cgcatcatca tatgtatatt
     1081 ccattttttc atgaaaattt ttgaaatttt tcgaaatatt ctctttgata agctttgcct
     1141 gcggcggagt gatggattta taatatctgg atatcgctgc atatccgcag ttcggacaaa
     1201 caatcacatc atatttcagc ggatcgatct tttcatattt cggacgcaga tccgtatccg
     1261 taccaagcag ctttgccttt ccgaccttga tcgtcctgac cttgaactct tcatcgcaga
     1321 ccggacacgt ataggattta tcaaataaaa aatccaattc ggtcatcact tttgccgtcc
     1381 cgctgtgttt cttttctttt tcggactctt tctcaaaaag ctccgtattc tcaaattttc
     1441 caagccccat attcccaagc cccgacaaaa ttcccatgat gatccctcct gtttatctct
     1501 tattattctt tgttttgtat gaaaaccggc cgttttggca gcggctctat ccattggccg
     1561 gcagcctgaa cgaactcttc atggatacga tcctgttaaa taccggcgca tgcggtttcg
     1621 aatctttggc atccgcacag aaaaatcctt ttcttacaaa ctgatagctg tcatatgctt
     1681 ctgcgtcctc aagattttgc tccaatcggc agtcctttaa aacagtcagc gaattttcat
     1741 tcagattcat actgccgtct tcttcattat aaacgccttt ttcctcatcg accaggttct
     1801 cataaagacg gacctcggct tttatcgcat gcggcgcgct cacccagtgg atcgtgccct
     1861 ttaccttccg tccgcaaaaa ccgcttccgc attttgtgtc cggatcataa gtgcaatgca
     1921 cggtttttac cataccgtcg gcatcctttt caaagccggt acacgttaca aaatacgcat
     1981 tcatcaggcg cacttcgttg cccggaaaca gacgaaaata ttttttcggc ggttcttcca
     2041 taaaatcctc tctgtcaata tataattctc tggaaaaagg taccattctt gttccaagag
     2101 cttcattttc caaattgttc ggcacttcca gatactctgt cctgccttcc tcataattgt
     2161 caatgatcag tttgacaggc tcgagcacag ccatcattct cggtttcttg agcttcagat
     2221 cttccctgat acaatattcc agcatcgcat agtcaacgga agactggctc tttgaaatac
     2281 cgcacagttc cacaaacatc ttgatggatt ccggtgtaaa tccccgtctt ctaagtgctg
     2341 cgatcgacac aagccgcgga tcatcccagc cgtcaacgac accgtcttca acaagctttt
     2401 tgatatatct cttgccggtc acgacatttg tcagataaag ctttgaaaac tcgatctgtt
     2461 ttggcggata ctcatatgca agttctctga cgacccagtc gtacagaggt ctgtgatcct
     2521 caaattcaag tgtacagata gaatgcgaca caccttcgat cgcgtcttcg atcggatggg
     2581 caaaatcata catcggataa atgcaccacc ggtcgccggt att
//
LOCUS       DAOSYE010000273         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30245_length_2614_cov_3.281360, whole genome shotgun sequence.
ACCESSION   DAOSYE010000273 DAOSYE010000000
VERSION     DAOSYE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30245_length_2614_cov_3.281360"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLU43_05655"
     CDS             complement(<1..341)
                     /locus_tag="PLU43_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO27930.1"
                     /translation="MTLQQLKYVVMVAEKGTISEAAKELFISQPSLTSAVKELERELQ
                     ITIFTRTNKGIVVSNEGNIFLGYARQVLEQANLLEERYLGEKRIKPMFSVSTQHYSFA
                     VNAFVDLIKEY"
     gene            complement(344..898)
                     /locus_tag="PLU43_05660"
     CDS             complement(344..898)
                     /locus_tag="PLU43_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOO27931.1"
                     /translation="MELMEGRPETNEGRLGKEIRVYDLLDALKISYQRVDHEAAETME
                     VCEPINEALAGAVICKNLFLCNTQKTKFYLLMIRGDKHFKTKEISHQISSPRLSFAPE
                     TYMEQYLDIMPGSVSVMGLMNDKEQHVQLLVDEDVLAEEFIGCHPCVNTSSLKLKTAD
                     VFGPFLNEIRHDYKKVILSDGKGN"
     gene            complement(978..>2614)
                     /locus_tag="PLU43_05665"
     CDS             complement(978..>2614)
                     /locus_tag="PLU43_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOO27932.1"
                     /translation="IKGFAAGCDLSMPGGSAYMEEACVEAVRSGRLFEACVDASVERM
                     LKLEKQCGIKIEDTPQADLPAGYALAKKIAAEGAVLLKNEDALLPLFNEEETAFIGYM
                     AKEMRYQGSGSSRINPWKQKNMIQVCRNARFAMGCNADGSTSDALLTEVRRAATEAKY
                     AVVFAGLPDSCESEGFDREHMRMPKGHIRMIEEAAKANENCIVVLLCGSVVELPWFER
                     VKAVLYMGLSGEAGAEAAADLLFGRENPCGKLAETWPLTYGDCVSAPYYSGTRKDAQY
                     REGLYAGYRYYETAGRPVRFPFGYGLSYTDFSYSDLKIEGTAVSCTVTNTGSRFGKEI
                     VQLYIKPQAAGVYRPVRELKGFSKIALAPGESGEVHFLLDERSFAVWMDGWKVQDGSY
                     QICIGKDCHTIVLSQETYVQGEQIAGQNVPAWYHAPAGSPSKEDFETLLGHAIVEKSL
                     KKGEFTMDNTVCEMKAYSLIMKIMYKAIEKTVAKGFGKKADYTDPTFRMIMSSSADCS
                     LTSMKISGGMNNHLLEGLLEMANGRFFKGVRLLLKK"
BASE COUNT          623 a          738 c          500 g          753 t
ORIGIN      
        1 tcatattctt tgatcagatc aacaaaagca tttaccgcaa acgaataatg ctgggtcgag
       61 acagagaaca tcggcttgat ccgcttttct ccaagatatc gttcttccag cagatttgcc
      121 tgttcaagca cctgccttgc atagccgaga aatatgttcc cttcattgga cacaacgatc
      181 cctttattcg ttcttgtaaa gatcgtgatc tgtagttctc tttccagttc tttaaccgca
      241 ctggtcagac tcggctgtga aataaaaagc tcttttgccg cttcgctgat cgttcctttt
      301 tcagcgacca taacaacata ttttaactgc tgcagtgtca taatcaattt ccttttccgt
      361 cactgagtat taccttttta taatcatggc ggatctcatt taaaaacggc ccgaatacat
      421 ccgctgtttt cagtttcagg ctggatgtat tgacgcacgg atgacagccg ataaattctt
      481 ccgccagcac atcctcatcc acaagcagct gcacgtgctg ctccttatca ttcatcagcc
      541 ccataacgct gaccgaaccc ggcataatat ccagatactg ctccatatac gtttccggcg
      601 caaaagaaag ccgcgggctg ctgatctgat gggagatctc ctttgtttta aaatgcttgt
      661 ctccccggat catcagcaga taaaattttg ttttctgcgt attgcataaa aacagatttt
      721 tgcagataac ggcgccggcc agcgcttcat tgatcggttc gcatacttcc attgtctcgg
      781 ctgcttcatg gtctactctc tgataactga tctttaatgc atccagaaga tcgtatactc
      841 tgatttcctt ccccagtctg ccttcgtttg tttccggtct tccttccata agttccattt
      901 ctgtcattaa ctccttactg tcgttttctc attaagacaa ttgtaagata aaagcaatgt
      961 aattacaaca attccttcta tttttttaac agcagacgta ctcccttgaa aaaacggccg
     1021 tttgccattt ccagcagccc ctccagcaga tgattgttca tgcctccgct tattttcatg
     1081 gatgtcaggg aacagtcggc agaggaactc atgatcatcc ggaacgtcgg atccgtatag
     1141 tcggcttttt ttccaaagcc cttcgctacg gtcttctcaa tagctttata catgatcttc
     1201 atgatcaggg aatatgcttt catttcacag accgtattgt ccattgtaaa ttcccctttt
     1261 ttcagagact tttccacgat cgcatggcct aaaagcgttt caaaatcctc tttagagggc
     1321 gaaccggccg gtgcatgata ccatgccgga acattctgcc ccgctatttg ttctccctgc
     1381 acataagttt cctgcgaaag cacgatcgta tgacagtctt tcccgatgca gatttggtaa
     1441 ctgccgtcct gaaccttcca gccgtccatc cataccgcaa agcttctttc atccagtaaa
     1501 aaatgaactt ctccggattc tcccggtgca agtgcgatct ttgaaaatcc cttcagctcc
     1561 cgtaccggac ggtaaacacc ggctgcctgt ggtttgatat acagctgcac gatctctttt
     1621 ccgaaccggc tgcccgtatt tgtcacagta cagctcacgg cagttccttc tattttcaga
     1681 tccgaatagg aaaaatccgt atacgacagt ccatatccga aaggaaaacg gaccggccgc
     1741 ccggctgtct cgtaatagcg gtatcctgca taaagtcctt cacgatactg tgcatctttc
     1801 ctggtaccgg aataatacgg tgcgctgaca cagtccccat aagtaagcgg ccatgtttcc
     1861 gccagcttcc cgcatggatt ttctctgcca aaaagcagat ctgccgccgc ctccgctcct
     1921 gcttcccctg acagtcccat ataaaggact gcctttacac gctcaaacca aggcagctcc
     1981 accacgcttc cgcaaagcag aacgacaata cagttttcat tggcttttgc cgcttcttct
     2041 atcatacgga tatgtccttt cggcatacgc atatgttccc tgtcaaagcc ctccgactca
     2101 cagctgtccg gcagaccggc aaatacaact gcgtatttcg cttctgtggc tgcacgccgg
     2161 acttctgtca acagcgcatc cgatgtactg ccgtctgcgt tgcagcccat tgcaaaacgc
     2221 gcattcctgc atacctgaat catatttttc tgcttccatg gattgatccg gctgctgcct
     2281 gaaccctgat aacgcatttc ttttgccata tatccgataa acgcagtctc ctcctcgtta
     2341 aaaagcggga ggagggcatc ttcatttttt aaaaggaccg caccttctgc tgcaatcttt
     2401 tttgcaaggg catagccggc aggcagatct gcctgcggag tatcttctat tttaatgccg
     2461 cactgctttt ccagcttaag catcctttca acagaagcat ccacacatgc ttcaaaaagc
     2521 cggccgctcc tgacagcttc cacacacgct tcttccatat acgcagagcc gcccggcata
     2581 gacagatcac agccggccgc aaagcctttg atac
//
LOCUS       DAOSYE010000274         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30357_length_2608_cov_3.165296, whole genome shotgun sequence.
ACCESSION   DAOSYE010000274 DAOSYE010000000
VERSION     DAOSYE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30357_length_2608_cov_3.165296"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLU43_05670"
     CDS             complement(<1..733)
                     /locus_tag="PLU43_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27933.1"
                     /translation="MSHTKKNPVKGILDIRGMRGALTAFAGLIIIYIAFGIINPTVFT
                     GQNVMNLLRSMSKYLLIGIGQSYILITGNIDLSIGSMVGMSAMITATLMTNGVAPMIA
                     ILVALCCCLLIGVINGILVGNFKLPPFIATLGTMFVARGVAYMVNGNRNTDAISSGIG
                     KEKADAFQSIFYYGKTLGIYNTFWIAIAIFIVFFFLLSKTRSGRHIYAIGSNIDAAQL
                     SGVNVVATVTKAYLVSAFCSFVVGLI"
     gene            complement(748..2262)
                     /locus_tag="PLU43_05675"
     CDS             complement(748..2262)
                     /locus_tag="PLU43_05675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO27934.1"
                     /translation="MNETIALMDNISKFFPGVKALDKVHFELRSGEVMALLGENGAGK
                     STLMKILSGVYTKDDGTVEIFGKEYENLTPKQAQEAGVAIIHQELNMCRHLSITENMF
                     LGREVRKGIVLSNSEMEEEAQHVLDELKIDLDPKKIVGDLPVSKQQMVEIAKALSTNA
                     RILIMDEPTSALTAKEIEDLFRIIHDLKKKGCGIVYISHRLEELQQITDRVTIMRDGQ
                     YVASMDYKDTTMDEIIANMVGREIKEKFPKVECKKGEKVFEVRNLNRGRLVRNINFSV
                     YKGEIVGFAGLMGAGRTETTRAIFGIDEKESGEIILDGREVVIKRPKDAIRAGIVLAP
                     EDRKKDGLCTKLSIRDNIALPNLDFLCNRLGVVNKGKETKMCNQTVENLKVKTPNLEI
                     DAGNLSGGNQQKVVVGKWLARNSKVVIFDEPTRGIDVAAKVEIYHLMNKLKKQGIAVV
                     FVSSEMPEIMGIADRIIVMCDGRITGELMAKEATQNEILTMATKFENKFSGEEA"
BASE COUNT          653 a          696 c          461 g          798 t
ORIGIN      
        1 aaatcagacc tacaacaaac gaacagaatg cactgacaag atatgctttc gtcacagtcg
       61 ccaccacatt gacaccggac agctgcgcag catctatatt ggagccgatc gcataaatgt
      121 gcctgcccga ccttgttttt gacagtaaaa agaaaaacac gatgaaaatc gcaatcgcga
      181 tccagaatgt gttataaata ccgagtgtct ttccataata gaaaatactc tgaaacgcat
      241 ccgctttttc ttttccgata ccggagctga tcgcatcggt attgcggttg ccgtttacca
      301 tataggcaac tccccttgcc acaaacatcg taccgagcgt ggcaataaac ggcggaagct
      361 tgaaattgcc tacaagaatg ccgttaatga ccccgatcag aagacagcag cataacgcta
      421 ccaaaattgc gatcataggt gctactccat ttgtcatcag cgttgccgtg atcatcgcac
      481 tcatgcctac catggaaccg atcgacagat cgatatttcc tgtgatcaat atgtaactct
      541 gcccgatccc gatcagcagg tactttgaca tggaacgcag cagattcatg acattctgcc
      601 cggtaaatac cgtaggattg ataataccga atgcaatgta gataatgatc agacctgcaa
      661 atgcagtcag cgcccctctc attccgcgta tatccagaat ccctttcacg ggatttttct
      721 tggtgtgact catcttgttt tcctccttca tgcttcttca ccagaaaatt tattttcaaa
      781 ttttgttgcc attgtcagta tttcattttg tgttgcttcc ttagccatca gttcccctgt
      841 gatccgcccg tcgcacatga cgatgatccg gtctgcgatt cccatgatct ccggcatttc
      901 cgacgaaaca aatacgaccg cgattccctg ctttttcagc ttattcatca aatggtagat
      961 ctcgaccttt gcagcaacat cgattcctct tgtcggctca tcgaaaataa cgacctttga
     1021 atttcttgca agccatttcc caacaacgac tttttgctga tttccgccgg aaaggtttcc
     1081 ggcatcaatt tccagattcg gcgttttgac ctttaaattt tccacggtct gattacacat
     1141 ctttgtttct ttccctttgt tcacaactcc gagccggtta cacaaaaaat ccagattcgg
     1201 cagtgcaata ttatccctga tactgagctt tgtacaaagt ccatcttttt tcctgtcctc
     1261 cggtgcaagt acgataccgg ctctgatggc atccttcggc cgtttgataa cgacctctct
     1321 cccatccaga ataatttccc cgctttcttt ttcatcaatt ccaaagatcg ctcttgtcgt
     1381 ctctgtcctg ccggcaccca tcagtcccgc aaagcctact atttcgcctt tatatacgga
     1441 gaaattgata tttctcacaa gtcttcctct gttcagattt cttacctcaa atactttttc
     1501 tccctttttg cattctacct tcggaaattt ctccttgatc tcacgtccga ccatattggc
     1561 tatgatctca tccatcgtcg tgtccttgta atccatggac gcgacatact gaccgtcccg
     1621 catgatcgtt accctgtccg taatctgctg cagttcttcc aggcggtgcg aaatatatac
     1681 gattccgcag ccttttttct tcagatcatg gatgatccga aacagatctt cgatctcctt
     1741 tgccgtcagg gccgaggtcg gttcgtccat gatcagaatt ctggcatttg tcgaaagtgc
     1801 tttcgctatt tccaccatct gctgctttga caccggcaga tccccgacga tctttttcgg
     1861 atccagatca attttcaatt catcaagaac gtgctgcgcc tcctcttcca tctcactgtt
     1921 tgaaagcacg attcccttcc tcacctctcg cccgagaaac atattttcgg taatagaaag
     1981 atgcctgcac atatttaact cctgatgaat aattgcaact ccggcttcct gtgcctgctt
     2041 cggcgtcaga ttttcatact cttttccaaa gatctcaacc gttccgtcat cttttgtata
     2101 aacaccgctc aaaattttca tcagggtgct ctttccagca ccgttttctc caagcagtgc
     2161 catcacttca ccggaacgca gttcaaaatg tactttgtca agcgccttta cccccggaaa
     2221 gaatttgctg atgttatcca tcagagcaat tgtctcattc attttcgtca cctgttcctt
     2281 tttcctgtat cctttccgca ttttttgttc aatcagctat cttttctccc attatagcaa
     2341 agatgctttg tgcaaataag gtgccattct ttctttttaa ggggggattt ttatgaagta
     2401 tgcacaacta cgatcatttg acatcaaaaa aacagcagcg gcacaaaacc gatgctgtcc
     2461 atatcgtttt tttaattttc cctgaagtat ttataaaatg tcactaaata aagaattgct
     2521 gaaagaatgg cggccgcata atcggtgacc ggctgcgcca taacaagcat tttggcagaa
     2581 tcaaagattt cattaaacag aaacagaa
//
LOCUS       DAOSYE010000275         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30401_length_2606_cov_2.907879, whole genome shotgun sequence.
ACCESSION   DAOSYE010000275 DAOSYE010000000
VERSION     DAOSYE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30401_length_2606_cov_2.907879"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PLU43_05680"
     CDS             complement(<1..1303)
                     /locus_tag="PLU43_05680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOO27935.1"
                     /translation="MKVAVVGTGYVGLVTGTCFSDMGNDVWCVDVDENKIDRLKNGII
                     PIYEPGLEEMVKRNYAHGHLKFTTQIEEALKQTDICFIAVGTPMGEDGSADLRYVLDV
                     ACSIGRHMEHHMYVVDKSTVPVGTAVKVKETIQNELKRRKSPLTFDMISNPEFLKEGT
                     AVGDCMRPDRIVIGVSNSRAAEVMQELYMPYIRNTENFIIMDIASAEMTKYTANAMLA
                     TKISFMNEISNICDMVGADINKVRRGIGSDQRIGYHFIYAGCGYGGSCFPKDVQALIR
                     TSHQHGYEPQLLESVEAVNQRQKKVIPRKVTERFGEDLTGFVFAVWGLSFKPDTDDMR
                     ESAAITIINELTAHGAKIIAYDPKAMEEAKYCYLKDNDAVMYADSKYEALKGADALIL
                     VTEWKEFRSPDLDEIKYRLKNPIVFDGRNQYDKKRMRQEGFE"
     gene            complement(1345..>2606)
                     /locus_tag="PLU43_05685"
     CDS             complement(1345..>2606)
                     /locus_tag="PLU43_05685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO27936.1"
                     /translation="GFDDETTARYYEPRLTSIAREQYKSGYSACQKLIEGLRDSEKGE
                     TKTLRTQLMKRESCGCRCRDIIDNVKFRKKHFHKEENMERFMQETREMAIDLTTVESL
                     TGLKEALKKYVQHISCEYFYLLLCKEWEGYDRNIDQYHLTEKNDNYLKEGYGEDSELV
                     FGYSSNEFIEKCEINLQMLLEKLKEGQLGRNVFTISPLHYRDRCFGYCIIGNSDFPFE
                     NQLFYTWTMNIGNAIETIREQHLLRAMIKKLDLMWIYDNLTGLYNRAGFHKYGEYVWQ
                     ECVQKNETAMLLFLDMDGLKYVNDTYGHDEGDRFIKVIAGILKRCKRHGEIVMRYGGD
                     EFVVLASGISEKKAEEYRDRILYGMEDYNSCHQLPYQIAASIGIYISTPEKRSDLEEA
                     IEEADRRMYEVKREKKCHRSQEFRSGL"
BASE COUNT          634 a          728 c          447 g          797 t
ORIGIN      
        1 actcaaaccc ttcctgccgc atcctttttt tatcatactg atttcgtccg tcaaatacga
       61 tcgggttttt cagccggtac ttgatctcat ccagatccgg acttctgaat tctttccact
      121 cggtaacaag gatcagggca tccgccccct ttaaggcttc atatttactg tctgcataca
      181 taactgcatc attatctttc agataacaat acttcgcctc ttccattgct tttggatcat
      241 aggcaatgat ttttgcaccg tgcgccgtca gctcattgat gatcgtaatg gccgcagatt
      301 ccctcatatc atccgtatcc ggtttaaagg aaagccccca tacggcaaaa acaaagccgg
      361 tcaggtcttc tccgaagcgt tccgtcactt tccttggaat cacctttttc tgtctctgat
      421 tgactgcttc gaccgattcc agcagctgcg gttcatatcc atgctgatga cttgtcctga
      481 tcagagcctg tacgtctttt ggaaaacagc ttcccccata gccgcatccg gcataaataa
      541 aatgataacc gatacgctga tcacttccaa tgccccggcg gactttattg atatcggccc
      601 cgaccatatc gcatatattg gagatctcat tcataaacga gatctttgtc gcaagcatcg
      661 catttgccgt atattttgtc atttcagcgc ttgcgatatc cataataata aaattctccg
      721 tatttctgat ataaggcata tacagttcct gcattacctc agctgccctg gaattgctga
      781 caccgatcac gatccggtca ggtctcatac aatcaccgac agcagtcccc tcctttaaaa
      841 actccggatt ggaaatcata tcaaaggtca gcggactttt tcttcttttc agctcgttct
      901 gtatcgtttc ctttaccttt acggctgttc cgacaggcac ggtcgattta tcgaccacat
      961 acatatgatg ctccatatgc cgcccgatac tgcatgcgac atccaataca tagcgcagat
     1021 ccgcactgcc gtcttctccc atcggagtcc cgaccgcaat aaaacagata tccgtctgct
     1081 tgagtgcctc ttctatctgt gtagtaaatt tcaaatgtcc atgtgcataa ttccgcttta
     1141 ccatttcctc aagacccggc tcgtagatcg gaatgatccc gttctttagc ctgtcgatct
     1201 tattctcatc cacatccaca caccatacat catttcccat atccgaaaaa caggtccccg
     1261 tcacaagtcc tacataaccg gtacctacaa ctgctacttt cattctttta tcctctttct
     1321 cttttctttc tatagataaa tcaactacag accggagcga aactcctgac tgcggtgaca
     1381 tttcttttcc ctttttacct catacattct cctgtcggct tcttcgattg cctcttccag
     1441 atcggatcgt ttttccggtg tggaaatata aatgccgata ctggcagcta tctgatacgg
     1501 cagctgatgg cagctgttgt aatcttccat cccataaagg atccggtccc tgtattcttc
     1561 cgctttcttt tcagaaatac ccgaagccag gacaacaaac tcgtctccgc catagcgcat
     1621 aacgatctca ccatgacgct tacagcgctt caggatgcct gcgatcacct tgataaagcg
     1681 gtcgccttca tcatgcccat atgtgtcatt gacatattta agtccatcca tatcgagaaa
     1741 cagcagcatg gccgtctcat ttttctgtac acactcctgc catacatatt ccccgtattt
     1801 gtgaaaaccc gcccggttat aaagtcctgt caggttgtcg tagatccaca tcagatccag
     1861 tttttttatc atggctcgca gcaggtgctg ttccctgatc gtttcgatcg catttccaat
     1921 attcatcgtc cacgtataaa aaagctgatt ttcaaacgga aaatcactgt tgccgataat
     1981 gcagtaccca aaacagcggt cacgataatg aagcggagag atcgtaaaaa catttctgcc
     2041 aagctgtcct tcctttagct tttcaagaag catctgcaga ttaatctcgc atttctcaat
     2101 aaactcatta ctggaatacc cgaagactag ctcactgtcc tcaccatatc cttccttcag
     2161 ataattatca ttcttttccg tcagatgata ctgatcgata ttcctgtcat agccttccca
     2221 ctctttgcat aacagcaaat aaaaatactc acagctgatg tgctgtacat attttttcag
     2281 cgcttcctta agacccgtca gggattccac agttgtaaga tcgattgcca tttcccgcgt
     2341 ttcctgcatg aagcgttcca tattttcttc cttgtgaaaa tgttttttac ggaactttac
     2401 attatcaata atatcacggc atctgcatcc gcagctctct ctcttcataa gctgcgtacg
     2461 gagcgtctta gtctctcctt tttcagagtc ccgcagaccc tctatcagct tctggcatgc
     2521 ggagtaccct gacttatact gctctctcgc aatacttgtc agacgcggtt cataataacg
     2581 tgccgttgtc tcatcgtcaa accctg
//
LOCUS       DAOSYE010000276         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30462_length_2603_cov_2.784537, whole genome shotgun sequence.
ACCESSION   DAOSYE010000276 DAOSYE010000000
VERSION     DAOSYE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30462_length_2603_cov_2.784537"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="PLU43_05690"
     CDS             <1..1576
                     /locus_tag="PLU43_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOO27937.1"
                     /translation="FGEKQKAAAVKRNHKPAEITDIREEISHRERSGKHKNYQFPPVT
                     LLKKGNINSNVDSTRQLKETAARLQQTLGNFGVHVTITDISQGPTVTRYEMQPEQGVK
                     VSKIVSLTDDIKLNMAATDIRIEAPIPGKAAVGIEIPNKENSTVMFRDLIESSEFRHS
                     QAKLSFAVGKDISGQIVVTDIAKMPHLLIAGATGSGKSVCINTLIMSILYKTNPDDVK
                     LIMVDPKVVELSIYNGIPHLMIPVVTDPKKAASALHWGVSEMTDRYNKFAELGVRDLK
                     GYNKKIETMQNVPEEERQKKLPQVIIIVDELADLMMVASNEVEESICRLAQLARAAGI
                     HLIIATQRPSVDVITGLIKANMPSRIAFSVSSGVDSRTILDMNGAEKLLGKGDMLFYP
                     QGYAKPARIQGAFISDQEVSDVVDFLKDHTGGEGYRADIEEKITSGIASASFGAGAAS
                     DRDQYFTEAAKFIIDKDKASIGMLQRVFKIGFNRAARIMDQLAEAGVVGEEEGTKPRK
                     VLMSMEQFEQFMEDSL"
     gene            1620..>2603
                     /locus_tag="PLU43_05695"
     CDS             1620..>2603
                     /locus_tag="PLU43_05695"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOO27938.1"
                     /translation="MKSDIQIAQEAELQPIKTVAAKLDIDEDDLELYGKYKAKLSEEY
                     LSKIKDRPNGKLVLVTAVNPTPAGEGKTTTSIGLAQAFAKLDKKAILALREPSLGPCF
                     GIKGGAAGGGYSQVVPMEELNLHFTGDFHAVTAANNLLSALLDNHIQQGNEKRIDTRN
                     IVWKRCVDINDRVLRNVVVGLGQKTDGFVREDHFVITVASEIMAILCLAENMSDLKKR
                     LARIVVAYDLDGNPVTAEDVRAVGSMAALLKDAIKPNIIQTLEHTPALVHGGPFANIA
                     HGCNSVRATKAALKMADYVITEAGFGADLGAEKFFDIKCRQAEIKPDAAVLV"
BASE COUNT          796 a          499 c          687 g          621 t
ORIGIN      
        1 gtttggggaa aaacagaaag cggccgcagt caaaagaaat cataaacctg cggaaatcac
       61 tgacattcgg gaagaaatat cacataggga gagatcaggg aaacataaaa attaccagtt
      121 tcctccggtt actcttttga aaaaaggaaa tataaacagt aatgtggatt ccacaagaca
      181 gttaaaggaa actgcggcca ggctgcagca gacgcttggt aatttcggtg ttcatgtaac
      241 aattacggat atcagtcagg ggccgactgt tacaagatat gaaatgcagc cggaacaggg
      301 tgtaaaagtc agtaagatcg ttagtctgac cgatgatatt aaattgaata tggcagcgac
      361 agatattcgg atcgaagcgc cgattccagg caaagcggca gtgggtattg aaattccaaa
      421 taaagaaaac agcacggtca tgttccggga tttaatagaa agttcggaat tccggcactc
      481 acaggcaaaa ctcagttttg cggtaggaaa agatatcagc ggacagatcg tagtcacgga
      541 tattgccaag atgccgcatc tgcttattgc aggtgcaaca ggttccggaa aatcggtctg
      601 tattaataca ctgatcatga gcattcttta caagacaaat cctgatgatg tcaaactgat
      661 catggtagat ccgaaggttg tggagctgag tatttataat gggatccccc atttaatgat
      721 tcctgtcgta acagatccta aaaaagcagc atcggcactt cactggggag tcagtgaaat
      781 gacggaccgt tataataaat ttgccgaact gggtgtccgc gacctgaaag gttataacaa
      841 aaaaatagaa accatgcaga atgtgccgga agaagaaagg cagaaaaagc ttccgcaggt
      901 cattattatt gtggacgagc tggccgacct tatgatggta gcttccaatg aagtggaaga
      961 atcgatctgc cgtcttgcac agcttgcaag agctgccggc attcatctga ttattgcgac
     1021 acagcggcct tccgtagatg ttattaccgg tctgatcaag gcaaatatgc caagcaggat
     1081 cgcattcagc gtatcaagcg gtgttgattc caggacaatc cttgatatga acggtgcgga
     1141 gaaactgctc ggaaaagggg atatgctttt ttatccgcag gggtatgcga aaccggcgcg
     1201 tattcagggc gcattcatat ccgatcagga agtgtcggat gttgtggatt ttctgaaaga
     1261 tcatacaggc ggtgaaggat atcgtgccga tattgaagaa aaaataacgt ccggaatagc
     1321 ttccgcatca tttggcgcag gcgcagcttc ggacagggat cagtatttta ccgaggcggc
     1381 taagtttatt attgataaag ataaagcgtc tatcggcatg ctgcagagag tgttcaagat
     1441 cggttttaac cgtgccgcaa gaatcatgga tcagctcgcg gaagcaggtg tcgttggtga
     1501 agaggaaggc acgaaacctc gtaaggtttt aatgagtatg gaacagttcg aacagtttat
     1561 ggaagattcc ctataacgaa gaactgagat aataatgtaa gaaagcgtga ggtgtacaga
     1621 tgaaatcaga tattcaaatt gcacaggaag cagaactcca gccaattaaa actgtagctg
     1681 caaagctgga tattgacgag gatgatttgg agctatacgg taaatataaa gccaaattgt
     1741 ctgaagaata tctttcaaag atcaaggacc ggccaaatgg aaaactcgtt cttgtaacag
     1801 ccgttaatcc gacaccggca ggagaaggaa aaacaacaac aagtatcgga cttgcgcagg
     1861 cattcgccaa actggataaa aaagcgatcc ttgcgcttag ggagccatcg cttggtccat
     1921 gcttcggtat taaaggcggg gcagccggag gcggttattc acaggtcgta ccgatggagg
     1981 agctgaatct gcattttaca ggggattttc acgctgtcac tgcagcaaac aatctgcttt
     2041 ctgcgttact tgataatcat atccagcagg ggaatgaaaa acggatcgat acaaggaata
     2101 ttgtctggaa gcgctgcgtg gatatcaatg accgggtgct tagaaatgtt gttgttggcc
     2161 ttggacagaa gacggacgga tttgtcaggg aagaccattt tgtcataacg gtcgcttcag
     2221 aaatcatggc gatcctgtgc cttgcggaaa atatgagtga tctgaaaaaa cgtcttgcac
     2281 ggatcgttgt tgcatatgat cttgacggaa atccggttac ggcggaagat gtcagggcag
     2341 taggttccat ggcagcactt ttgaaagatg ccatcaaacc aaatattatt cagacgctgg
     2401 aacatacgcc ggcacttgta catgggggtc cgtttgcaaa tattgcgcat ggctgtaaca
     2461 gcgtacgtgc gacaaaggca gcattgaaaa tggctgacta tgttattacg gaggcaggct
     2521 ttggtgcgga tctcggcgca gagaaatttt ttgatatcaa gtgcagacag gctgaaatca
     2581 agccggatgc agccgtgctg gtc
//
LOCUS       DAOSYE010000277         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30476_length_2602_cov_3.632116, whole genome shotgun sequence.
ACCESSION   DAOSYE010000277 DAOSYE010000000
VERSION     DAOSYE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30476_length_2602_cov_3.632116"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLU43_05700"
     CDS             complement(<1..963)
                     /locus_tag="PLU43_05700"
                     /inference="COORDINATES: protein motif:HMM:NF039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HOO27939.1"
                     /translation="MLRILTEIGCVIANLIIYLAFGTLITRKRGREWPLGLSVIIGFF
                     FYYSLFFFFCVPIMIRFRPLSMLTKLWVPAVCVVVLLSCILNGKAWYEKCRQMAGKIK
                     AHPVFYFLIAALIIIQVMIVTRAYNFTLDAAYYVANVATNVETNMMNVYDPFTGAWLD
                     HFEMRYFFATYQISDAVVCQLTGIPALIQTKTIMAAVIIILTNLLYYLIAQALFLDRE
                     KSVVIMLAFIFLINLTFITIYTSSLFLMTRTYEGKAIVGNLSLLAVFYLYMRLLSSEH
                     TPRIWLMLFLVCFGSMTISSSANMLLPAELSVLLLPLAFMKKNWR"
     gene            complement(963..2267)
                     /locus_tag="PLU43_05705"
     CDS             complement(963..2267)
                     /locus_tag="PLU43_05705"
                     /inference="COORDINATES: protein motif:HMM:NF039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HOO27940.1"
                     /translation="MIFWIKLSLLAGSFLLAGCFTGYLAVSAGKFREKSGFLLIVSGM
                     VCNICLFAVLSTICSLAGLWLAVPGVIWLLLLAGTAVYVLLRSGRSFFSYIKTGFLNW
                     MQRMKNKTDWFSLLIIVLSLLLIALQICGTAFFSYHQPNATRQIQDATRAYETGVLSV
                     SSPMMMLWAWTAKLIGEHPLTVIFSMSQFLTIPLYYMGYALLAGKLCKDNRLQSLLLV
                     FFMCILHLFGYQSGYALNMTLLFSYFSGEVFILQGLLPYLLWLLLTAYDRFAKAADGK
                     EENKQTDLESDWEDEDMRKHKVLNARNLGIALLVFIVLFAGAVYILNHKINSLYYATQ
                     NLQTAMDEKCSIYEFRLKDSGETAGYLLRQSDGSLVMIGGGPAENGEELYEFLSKYGT
                     KLDQWYLYGETDENTGAYEYCTDKKEIEAGKVYYLTGMEEVK"
     gene            complement(2264..>2602)
                     /locus_tag="PLU43_05710"
     CDS             complement(2264..>2602)
                     /locus_tag="PLU43_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27941.1"
                     /translation="LDDCTDPKVIAPPGFAPYLKMYSSHFETLYDYPKNGDISRLSND
                     ARQVYEQLSGSIPDTKLIADIAKENDCRYVIMDSTKYYPEFPITAFGFELENTVGDWK
                     IYKIEEGNGL"
BASE COUNT          773 a          670 c          516 g          643 t
ORIGIN      
        1 cctccagttt tttttcatga acgcaagcgg cagtaaaagt acggaaagct ctgccggcag
       61 cagcatattg gctgaggatg agatcgtcat cgagccaaag cacacaagga acagcatcag
      121 ccagatacgc ggcgtatgct cactggaaag cagccgcata tataaataaa acacagcgag
      181 cagggataaa tttccgacga tcgcctttcc ttcatacgtt cgggtcatca aaaacaggga
      241 ggatgtataa atcgtaataa atgtgagatt gatcagaaat ataaatgcaa gcatgatcac
      301 aactgatttt tcacggtcca aaaacagagc ctgtgcaatc agatagtaca gcagatttgt
      361 cagtatgata atgaccgcgg ccatgatcgt ctttgtctgg atcagggcag gtattcctgt
      421 cagctggcag acgaccgcat cgctgatctg ataagtggca aaaaagtacc gcatctcaaa
      481 atgatccagc catgcgccag taaacggatc gtagacgttc atcatatttg tttctacgtt
      541 cgtcgcaaca ttcgcaacat aataggcagc atccagcgta aaattatagg ccctcgtcac
      601 gatcattacc tgtatgatga tcagtgcggc aatcagaaaa taaaataccg gatgggcttt
      661 tatttttccg gccatctgcc tgcatttttc ataccacgct tttccgttca gaatgcagga
      721 gagaagcaca acaacgcaga cagccggcac ccacagcttt gtcagcatcg acaaggggcg
      781 gaaccggatc atgatcggta cgcagaaaaa gaaaaagaga ctgtaataaa agaaaaagcc
      841 gataataaca ctcagtccga gcggccactc ccgcccccgc tttctcgtta tcagtgttcc
      901 aaacgccaga taaattataa gattagcaat cacacatccg atttccgtaa gtatccgcag
      961 cattatttta cctcttccat tcccgtcaga taataaacct tccctgcttc tatttccttt
     1021 ttatcggtac aatattcata agcgcccgta ttttcatccg tttccccata caaataccac
     1081 tgatcaagct ttgttccgta tttcgataaa aattcataca gttcttcccc gttttccgca
     1141 ggaccgcctc cgatcataac aagagagcca tcgctctgcc gcagcagata tcctgccgtt
     1201 tccccactgt ctttcagcct gaattcataa atactgcatt tttcatccat ggctgtctgc
     1261 aggttctgtg tggcataata caggctgttg attttgtgat ttaaaatata gactgccccg
     1321 gcaaagagca ctataaagac aagcagcgca atccccagat ttcttgcatt caatacttta
     1381 tgctttctca tatcctcatc ctcccagtca gactccaaat ctgtctgttt attctcttcc
     1441 tttccgtccg ccgcttttgc aaaacgatca taagcagtca gcagcagcca taaaagatac
     1501 ggcagcagtc cctgcagtat aaagacctcg ccggaaaaat aactgaacag caatgtcata
     1561 ttcaacgcgt atcccgactg atatccgaac agatgaagga tacacataaa aaaaacgagc
     1621 agaaggcttt gaagccgatt gtctttgcac agcttccctg caagcagggc ataccccata
     1681 taataaagcg gaatggtaag aaactgagac attgaaaaaa taacggtcag cggatgttct
     1741 ccgatcagct ttgcagtcca tgcccacagc atcatcatcg gagaagatac ggaaagcacg
     1801 cctgtctcat atgctcttgt cgcatcctgg atctgtcttg tggcattcgg ctgatgatag
     1861 gaaaaaaatg ctgtcccgca tatctgcaga gcgatcagca gtaaactcag cactatgatc
     1921 agcagggaaa accagtccgt tttatttttc atccgctgca tccagttcaa aaaacctgtt
     1981 tttatatagg aaaaaaatga tcgcccgctg cgaagcagta catagactgc agtccccgca
     2041 agcaaaagca gccagatcac gccgggcact gcaagccaaa gcccggccag cgaacagatc
     2101 gtgctcagca ccgcaaacag acagatgttg cagaccatgc cgcttactat taacaaaaac
     2161 ccgctctttt cccggaattt cccggcagat actgccaaat atccggtgaa acagcctgcc
     2221 agcaaaaagg aaccggcaag cagactcagc tttatccaaa atatcataga ccgtttcctt
     2281 cctctatctt gtatattttc cagtcaccga ccgtattttc aagctcaaag ccgaatgccg
     2341 tgatcggaaa ttccggataa tactttgtcg aatccataat cacataccgg cagtcatttt
     2401 cctttgcaat atccgcaatc agctttgtat ccggaatcga accggacagc tgttcatata
     2461 cctgtctcgc atcattagaa agcctggaaa tatcgccatt tttcggatag tcatacaatg
     2521 tctcaaaatg ggaactgtac attttcagat aaggggcaaa ccccggcggc gcaatcactt
     2581 tcggatcagt gcaatcatcc ag
//
LOCUS       DAOSYE010000278         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30541_length_2599_cov_3.671384, whole genome shotgun sequence.
ACCESSION   DAOSYE010000278 DAOSYE010000000
VERSION     DAOSYE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30541_length_2599_cov_3.671384"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLU43_05715"
     CDS             <1..155
                     /locus_tag="PLU43_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO27942.1"
                     /translation="TELMATYMYKTAFTTYNMGYGSAIAAGMFILITLIASVLMKFMN
                     RGGDKQ"
     gene            152..1015
                     /locus_tag="PLU43_05720"
     CDS             152..1015
                     /locus_tag="PLU43_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO27943.1"
                     /translation="MSNEKKHEDHMQKKPLAARSLPVIFAILWLFVAFIPFLFMVLTG
                     FKQKLETIMYGAFHVPEEFYPDNYISIITSASFWVYFRNSVIVLVLVLVILLFVSACA
                     AYPLSRFQFKLRGSIYVLIVAMMSVPMHVTLIPVFKLTINIGLYDTIWALIPPAIAFA
                     TPISVFILTAFMKGIPKEIEEAAEIDGCGKVRAFFEIILPLSKPGLSTLAIYNGVNVW
                     NEFVFAYTLTQSENHRTLPLALWNYQGQYSSNTAMIMACLSLSVLPMIILFIFMQDKL
                     VKGMMAGAVKG"
     gene            1161..>2599
                     /locus_tag="PLU43_05725"
     CDS             1161..>2599
                     /locus_tag="PLU43_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO27944.1"
                     /translation="MKIFKNRTKIKKMTVDTRISLLLVLSVGIVTFLILLFTTLSTVR
                     SVTQKSTELIMKNVYSNAIDLDYILSSYENTLQSIETNAIVQSYLKVQKDGEYPSNAT
                     TVTDTLANTRNVSPDIWGITLIREGDSAFINSSVKPSSYMSGNFLEELMQNYEESLSL
                     QKGSMTYNVQQVYGEDGLYTLNIYRPVFNSIKIGDEIGLLCLRVDLSVVRKNFTNTGG
                     DITERIIGTDGQIIVPAESDEYPSSISNIEKIEGDSGQFTIHGVTTIYQKIEDRNLYL
                     VSEVPDYFFYDDAYTIMFLLLGLTAVFLLIMVFVSRRMIREIFEPFHKLREGMNRVSV
                     GDLCIRLSEEEGGEDVRELTAGFNNMAEKINAQMKEIVEKEREAQKNEIEALQESIKP
                     HFLYNTLECIHWETVAEGNMKASKMVQALAKYYRISLSKGRELITLSLELEHVRSYVM
                     IQNMRFNDTIRFLVNIPEDMQDLMVPKIT"
BASE COUNT          728 a          486 c          621 g          764 t
ORIGIN      
        1 ctaccgaatt gatggcgacc tatatgtata aaacggcatt tacgacctat aacatgggtt
       61 atggttctgc cattgcggcc ggtatgttta ttttgatcac gctgattgcg tccgtcctga
      121 tgaagtttat gaacagaggg ggtgacaagc aatgagcaat gagaagaaac atgaggatca
      181 tatgcagaag aaaccgcttg ctgccagaag tcttcccgta atttttgcga ttctgtggct
      241 gtttgtggca tttattcctt ttttgttcat ggtgctgacc ggattcaagc agaagctgga
      301 aaccattatg tatggtgcgt ttcatgtgcc tgaagaattt tatccggaca attatatttc
      361 tatcatcaca agcgcttcct tctgggtcta tttcagaaac agtgtgatcg tactcgtact
      421 cgttctggta atcctgttgt ttgtatcggc ctgtgctgca tatcctcttt cgcggttcca
      481 atttaaactg cgcggctcga tttatgtgct gatcgtggca atgatgtccg tgccgatgca
      541 tgttacgctg atccctgtat ttaagctgac gatcaatatt ggattgtatg atacgatctg
      601 ggcattgatc ccgccggcta ttgcgtttgc aacaccgatc tctgttttta ttctgacggc
      661 ttttatgaaa gggatcccaa aagagatcga ggaagctgcg gagatcgacg gctgcggaaa
      721 agttcgtgcg ttttttgaga tcattctgcc gttgtctaaa ccaggacttt ccacgttggc
      781 gatctacaat ggcgtaaatg tatggaacga atttgtgttt gcctatacac tgacacaatc
      841 ggaaaaccat cgtacgctgc cgctggcact ttggaattat cagggacagt attcatctaa
      901 tactgcaatg atcatggcat gtctgtcact ttcggttttg ccgatgatta ttttgttcat
      961 ttttatgcag gataagcttg tcaaggggat gatggcagga gctgtcaaag gctgattctg
     1021 caggtatcat attttttata aatagtgacc atacgaaaaa tttctgctac atgctgattt
     1081 tttaaatatc tattagtatt gtagcagttt tttttgttat aataaaatcg gaaataaccg
     1141 gaaagagatg gactcggccg atgaagatat ttaagaatag aacaaaaata aaaaaaatga
     1201 cggtagacac aagaatcagc cttctgctgg tcctaagtgt tgggatcgtt accttcttaa
     1261 ttcttctttt cacaacgctg agtaccgttc gttctgtcac acaaaaatcc acggaactga
     1321 tcatgaagaa tgtttacagt aatgcgatcg atctggatta tattttaagc tcctatgaaa
     1381 atacattaca gtccatagag accaatgcga ttgtccaaag ctatctgaag gttcagaaag
     1441 acggcgaata cccttcgaat gcgacgaccg tgacagatac actggcgaat acgcgtaatg
     1501 tttctcctga tatctgggga atcacgctta tacgggaagg ggacagcgct tttattaatt
     1561 ccagtgtcaa gccgagcagt tatatgagcg ggaattttct ggaagaattg atgcagaatt
     1621 atgaagaaag cctgtctttg cagaaaggtt ccatgacata caatgtacag caggtgtacg
     1681 gagaggacgg actttatacg ttaaatattt accggccggt attcaattcc ataaagatcg
     1741 gagatgagat cggacttttg tgtcttcgtg tcgatctttc ggtagtgaga aagaatttta
     1801 cgaatacggg cggagacatt acggaacgga tcataggaac ggacggacag atcattgttc
     1861 cggcggaaag tgacgaatat ccttccagta tttcaaatat tgaaaaaata gaaggggatt
     1921 ccggacagtt tacaatacat ggagttacaa cgatctatca gaagatcgaa gatcgtaatt
     1981 tatatcttgt cagtgaagtg cccgattatt ttttctatga tgacgcatat acgatcatgt
     2041 tcctgcttct tggactgaca gccgtctttc ttcttatcat ggtgttcgtt tcaaggcgta
     2101 tgatacgcga aattttcgaa ccgttccata aactgcggga aggaatgaac agagtttctg
     2161 ttggtgatct ctgcatccgt ttgtctgaag aagagggggg cgaggatgtc agggaactca
     2221 cggcaggctt caataatatg gcggagaaga tcaatgcaca gatgaaggag atcgttgaaa
     2281 aggaacggga ggcgcagaaa aatgagatcg aggctttgca ggaatcgatc aaaccgcatt
     2341 ttttgtacaa tacactggaa tgtattcact gggaaacggt tgcagaggga aacatgaaag
     2401 cgtccaaaat ggtgcaggca cttgccaaat actatcggat cagtctcagc aaagggaggg
     2461 agctgatcac tctttcgctg gaacttgagc atgtaagaag ctatgtgatg attcagaata
     2521 tgcgttttaa cgatacaatt cgttttcttg tcaatatccc ggaggacatg caggacctca
     2581 tggttcctaa aattacatt
//
LOCUS       DAOSYE010000279         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30592_length_2597_cov_3.247836, whole genome shotgun sequence.
ACCESSION   DAOSYE010000279 DAOSYE010000000
VERSION     DAOSYE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30592_length_2597_cov_3.247836"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLU43_05730"
     CDS             complement(<1..570)
                     /locus_tag="PLU43_05730"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO27945.1"
                     /translation="MYRDKTKVIKIGERVIGGGNPVLIQSMTNTKTEDIDATVAQIKR
                     LEAAGCEIIRSTVPTMEAAAAFSEIKKRIGIPLVADIHFNYKLAIAAIENGADKIRIN
                     PGNIGGRNNVTEVVKAARERGIPIRVGVNSGSLEKELVARYHGVTAEGIVESALDKVR
                     MIEDAGYDRIVVSIKSSDVMMCVKAHELLA"
     gene            complement(683..>2597)
                     /locus_tag="PLU43_05735"
     CDS             complement(683..>2597)
                     /locus_tag="PLU43_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27946.1"
                     /translation="VCFDLEAPALELKSDSDFEKWTSEDIDCILESRDDKSGIHTIEI
                     KTDEEFLFEKAFEQEEGREDRTEFLLDREAECVEGRELTIIVSDFAGNTETILERYYI
                     DKIKPTLLLSGISDGAIFQDPAVLDISVQDNLTENALLFYKAVKTVGGKEQILEEIPG
                     SADGSVINRIYEEDGMYELTYYAEDKAGNCSGLGTLRFRMDRTSPGIFVSGVLNGTDY
                     QTACSVNITVSESFFEDCHVEIKARRLTPGKEEAVSLPAWKTYGEDSENSCFFGEDGD
                     YEILIEAVDAAGNRAEKEVWFRIDQTAPLLEIEGLAEDTVTNIPPTITFHMEELFYDT
                     AAIQCLLLRKAEDGSYMPVMTPIWEPGSGNEDFLMEVKEEGCYELTAVATDRSNHRTE
                     KKLRFTLDYTPPVIGYLDTLHRKYFKSFRMPAHFASDITDLTQVNYAVYLNTRNLLPD
                     EEITEDGKYILRVEAADEAGNRSEKTVEFIVDQTMPHVVVKGTRKDGTVGRNEKLTLS
                     LYEEEDTFVSVFVNGIRQKLDGEKKEAVIRLLEYGDYELEVKAVDPAQNELTKIIKLK
                     CALAASPFTEYKVTEKIIRQEDVKTGRTEGKQWIILAAVLFVLAGTAVFFSCRLYRIH
                     KIKLKRNRSGPNE"
BASE COUNT          625 a          745 c          420 g          807 t
ORIGIN      
        1 cgcaagcagt tcatgtgcct tcacacacat catcacgtct gaggatttta tacttacaac
       61 gatccggtca tatccggcat cttctatcat ccttacttta tccagcgcac tctccacgat
      121 tccttcggct gttacaccat ggtatctggc tacaagctcc ttttccagcg aaccactgtt
      181 cacaccgacc ctgatcggaa taccccgctc tctcgcagct tttacaacct cggtaacatt
      241 atttctgccg ccaatatttc cgggattgat cctgatttta tcagccccgt tttcaattgc
      301 cgcaatggca agtttataat taaaatgaat gtccgcaacg agcggaattc cgatccgctt
      361 tttgatttcc gaaaaagccg cagccgcttc cattgtagga accgtactcc tgataatttc
      421 gcagcctgct gcctccagtc ttttgatctg tgccaccgta gcatcgatat cctcagtttt
      481 agtattcgtc atcgactgaa tcaataccgg attcccgccc ccgatcactc tttcaccgat
      541 tttaatcacc ttagtcttat cccgatacat aaaatacccc tttcagcaca caatacccac
      601 cacaagatgt tgtggcaggt atcccttttt catccaacat cagtctatca cagccacttt
      661 ttcatgtcaa cgaaagcagt cgttattcat tcggtccgct acggtttctt ttcaatttta
      721 ttttatgtat cctatataaa cggcagctga aaaatacagc tgtcccggcc agaacaaaaa
      781 gcacagccgc aagtatgatc cattgtttcc cttctgtccg gcctgttttc acatcctcct
      841 gtctgattat cttttcagtt accttatatt ccgtaaacgg acttgcagca agtgcacatt
      901 ttaatttgat tatttttgtc aattcattct gtgcaggatc aactgccttc acttccagtt
      961 cataatcccc atattctaaa agtctgataa cagcttcttt tttctccccg tcaagtttct
     1021 gcctgatccc gttcacgaaa acactgacga acgtgtcctc ttcctcataa agacttaacg
     1081 tcagcttttc atttctgccg accgttccgt cttttcgtgt tccttttact acaacgtgcg
     1141 gcattgtctg atccacaata aattccactg tcttttccga tctgtttcca gcctcatccg
     1201 ctgcttcaac acgcagaata tacttgccgt cctctgtaat ctcttcatcc ggcagtaaat
     1261 tgcgtgtatt taaatatacc gcataattta cctgtgtcag atccgtaata tccgatgcaa
     1321 aatgagccgg catcctgaaa cttttaaaat attttctgtg cagtgtgtcc agataaccga
     1381 tcaccggcgg cgtataatcc aatgtgaatc tcagtttctt ttccgtacga tgatttgatc
     1441 tgtctgtagc aactgccgta agttcatagc atccttcctc ctttacttcc atcaaaaaat
     1501 cttcgttccc gctccccggt tcccagatcg gagtcataac aggcatataa ctgccgtctt
     1561 ccgcttttct aagcagcaga cactgtattg cagccgtatc ataaaacagt tcttccatat
     1621 gaaacgtaat ggtcggcggt atatttgtta ccgtatcttc agcaagaccc tctatttcaa
     1681 gcagcggcgc agtctgatcg atccggaacc acacttcctt ttccgccctg ttgccggccg
     1741 catcaacggc ttcgatcaat atttcataat ccccgtcttc tccaaaaaaa caactgtttt
     1801 cgctatcctc tccatatgtc ttccatgccg gaagagaaac agcctcctct tttccaggcg
     1861 tcagccgcct tgcctttatt tccacatggc agtcctcaaa aaaactttct gaaacagtaa
     1921 tatttacgga acaggctgtc tgataatctg tcccgttcag cacaccgctc acaaaaattc
     1981 cgggcgatgt ccggtccatt cgaaaccgca gggtaccaag tcctgaacaa ttccccgctt
     2041 tatcttccgc ataataagta agctcataca tcccgtcttc ttcgtaaatc ctgtttatta
     2101 cgctgccgtc tgcagaaccg ggaatttcct ccaatatctg ttcctttccg ccgacggttt
     2161 ttacggcctt gtaaaacagc agcgcatttt ccgtcagatt gtcctgtaca ctgatatcta
     2221 atacagccgg atcctgaaaa atggctccgt ccgatatccc ggacaacagc agcgtcggtt
     2281 tgatcttatc aatataatat ctctccagaa tcgtttccgt attgccggca aaatccgata
     2341 cgatgatcgt cagttctctc ccttcaacgc attcggcctc cctgtccagc agaaattccg
     2401 tacgatcctc ccttccctcc tcctgttcaa atgctttctc aaataaaaat tcttcatccg
     2461 ttttaatttc tattgtatga attccacttt tatcatccct gctttccaat atgcagtcaa
     2521 tatcttccga tgtccatttc tcaaaatcac tgtccgattt taattccagg gcaggcgcct
     2581 ccaggtcaaa gcagact
//
LOCUS       DAOSYE010000280         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30768_length_2587_cov_3.159558, whole genome shotgun sequence.
ACCESSION   DAOSYE010000280 DAOSYE010000000
VERSION     DAOSYE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30768_length_2587_cov_3.159558"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PLU43_05740"
     CDS             complement(<1..2033)
                     /locus_tag="PLU43_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOO27947.1"
                     /translation="MNYGRRGARKKQRELSSPTTRIGKFFAVSIFKVMVFAIVAVGVV
                     GLCAGVGLYMGIIDTAPDMSNIDVSPAGYSTTVYDNEGNQITKLVAENSNRIYITIDK
                     IPEHLQHAFVAIEDERFYTHNGIDIKGILRAGITALTSGDLSQGASTITQQLLKNNVF
                     TTWTSESSKIEKFKRKFQEQYCAVQLEKVMDKDTILENYLNTINLGQGTLGVQAAALR
                     YFGKSASDLTISESAVIAAITQNPSKWNPISHPDNNAERRKEVLTKMRDQGYITQTEY
                     DEALADDVYSRIKNVDEQTEKNTIYTYFVDELTEQVINDLQEVKGYSYTQAYNALYSG
                     GLSIFTTQDPEVQDICDEEFANSDNYPESTELYLNYELTYEDADGNAVNFSSQNFESY
                     FKQQDSSFNMIFTSEEDAQAKIDEYKEAVEEPGYTFVAENMTLTPQPQASITVMDQTT
                     GEVLAIVGGRGEKAASLTLNRATDTKRQPGSCFKVVAVYAPALDSAGFTLASTQIDEP
                     YNYVDGRPVSNWYSTGYKGICTLRYGIEQSLNIVAVKTLTDITPQLGYDYLLNFGFTT
                     LVERRTESDGTVSSDITQALALGGVTDGVTNLELTAAYATIANKGTYTKPMFYTKIID
                     HDGNVLIDNTPKTRQVIKETTAFLLTSAMEDVVTKGTGAKVNFGNMAIAGKTGT"
     gene            2279..>2587
                     /locus_tag="PLU43_05745"
     CDS             2279..>2587
                     /locus_tag="PLU43_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOO27948.1"
                     /translation="MDGYRCIYKGGESEIEEKKSRFIATVRPVETEDEAVAFIGEMKK
                     KYWDATHNCSAFVIGKDAGLTRCSDDGEPGGTAGRPMLEILLSKEIRNAAVVVTRYFG
                     G"
BASE COUNT          637 a          566 c          641 g          743 t
ORIGIN      
        1 gttccggtct ttcctgcgat tgccatgttg ccgaagttga ctttggcacc ggttcctttt
       61 gtaacaacgt cttccattgc gctcgtcaga aggaaagcag tcgtttcctt aatgacctga
      121 cgcgttttcg gcgtattgtc gatcagcaca ttaccgtcat gatctatgat ctttgtataa
      181 aacatcggtt ttgtataagt tcctttattg gcgatcgtag cataagcagc ggtcagttcc
      241 aggtttgtca caccgtcggt gacaccgccg agtgcgagtg cctgcgtaat atctgaggaa
      301 acggttccgt ccgattccgt gcggcgttca acaagtgttg taaagccgaa attcagcaga
      361 taatcatagc caagctgcgg cgtgatatct gtcagtgttt tgacggcaac gatattcagt
      421 gactgttcga taccataacg gagtgtacag atccctttat aacccgtact gtaccagttt
      481 gaaacaggtc gtccgtccac ataattataa ggttcgtcaa tctgtgtgga agcaagtgta
      541 aagcctgcgc tgtcaagtgc gggcgcatag acggcgacta ctttgaaaca tgatccgggc
      601 tggcgctttg tatcggtcgc acggttgagc gtcaggctgg cggctttttc tccgcgtccg
      661 ccgacaatgg caagtacttc gccggtcgtc tggtccatta cggtaatgga agcctgcggc
      721 tgcggcgtca gtgtcatatt ttcggcgacg aacgtatatc cgggttcctc gacggcttcc
      781 ttatactcgt cgatcttggc ctgtgcgtct tcttcgcttg tgaagatcat attgaagctg
      841 gaatcctgct gtttgaagta ggattcgaaa ttttgtgagc tgaaattgac agcatttcca
      901 tcggcatctt cataggtcag ttcatagttc agatacagct ctgtggattc cggataattg
      961 tccgaattgg caaactcttc atcacaaata tcctgaacct caggatcctg tgttgtgaaa
     1021 atactcaggc cgccgctgta cagcgcatta taagcctgcg tataggaata ccctttgact
     1081 tcctgcagat cgttgatcac ctgctcggtc agctcatcca caaaataagt gtagatcgta
     1141 ttcttttcgg tctgttcatc tacgtttttg atgcgtgaat agacatcatc cgcaagcgct
     1201 tcgtcgtatt ctgtctgtgt aatatatccc tgatcgcgca tttttgtcag aacttcttta
     1261 cggcgttccg cattgttgtc cggatgtgag atcggattcc atttggaagg attctgtgtg
     1321 atggctgcga tgactgcgga ttcggagatc gtaagatccg atgctgattt tccgaaataa
     1381 cgcagggcag cggcctggac gccaagcgtt ccctgcccaa ggttgatcgt attcagataa
     1441 ttttccaaaa tcgtatcttt atccatgact ttttcaagct ggaccgcaca gtactgttcc
     1501 tggaatttac gcttgaattt ttcgatcttg gaagattcgg atgtccatgt tgtaaaaaca
     1561 ttatttttca gcagctgctg ggtgatcgta ctggcaccct gtgacagatc cccgcttgtc
     1621 agggcggtaa tgccggcacg cagtatgccc ttgatatcga taccgttgtg tgtgtaaaaa
     1681 cgttcatcct cgatcgcaac gaaagcgtgc tgcagatgtt cgggaatttt gtcgatcgta
     1741 atataaatac ggtttgaatt ttcagcgaca agctttgtga tctgatttcc ttcattgtca
     1801 tatacggttg tggaatatcc tgcaggggat acgtcgatat tagacatatc cggagcggta
     1861 tcgatgatac ccatatacag tccgacaccg gcacacagtc cgactacgcc gactgccaca
     1921 attgcaaata ccattacctt aaaaatagaa acggcgaaaa attttccgat tcttgtagtg
     1981 ggcgaagaaa gttcccgttg cttttttctt gccccgcgtc taccataatt catgagtatg
     2041 gcctccttat ttgaaataac ggccgctttg tccatacggc gggcagcagt aaagattcgc
     2101 gtaatccgtt atgattttgg tgattttgcc gataacctat tggtaggttc ataacttaat
     2161 tattatagca aagatttata tgtaaataaa gcaaaattat gcgaattcac atttaatgaa
     2221 agaaaaatta agaattggca tcgggcgcag ggaaagagag aaaggaaata ggaacggcat
     2281 ggatggttat cgttgtattt acaaaggcgg agagagtgag attgaagaaa agaaatcccg
     2341 gtttattgca actgtgagac cggtagagac agaagacgag gctgtggcct ttatcgggga
     2401 aatgaaaaaa aaatattggg atgcgactca taactgttcc gcttttgtga tcggaaaaga
     2461 tgccggactg acacgctgca gtgacgacgg ggagccgggc ggtacggcag gcagaccgat
     2521 gttggagatc cttctttcaa aggagatcag aaatgctgca gtcgtcgtca cacgctattt
     2581 cggcggg
//
LOCUS       DAOSYE010000281         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30771_length_2587_cov_2.934044, whole genome shotgun sequence.
ACCESSION   DAOSYE010000281 DAOSYE010000000
VERSION     DAOSYE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30771_length_2587_cov_2.934044"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="PLU43_05750"
     CDS             complement(<1..1952)
                     /locus_tag="PLU43_05750"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOO27949.1"
                     /translation="MEKINLKNPEYYYNRELSWLLFNERVLGEARDKTNLLFERLKFL
                     SITASNLDEFFMIRVASLKDMVNAEYHKTDIAGLTPEQQLDKLNEKTHELVGLQYSTF
                     NRSLLPLLSQKGIRFVPVHEKLTDEQAAYADRYFEENVYPVLTPMAVDSSRPFPLIRN
                     KSLNIGALLSKKGSKKSRGGREEPEFATVQVPAVLPRIVKLPGKEGQKTFILLEEIIE
                     RNMDKLFLNYDIICAHPFRIMRNADLTIDEDEAADLLKEIERQLKRRQWGEVIRLEVA
                     DDIDKKLLKTLRKELDIRNEEIYRINGPLDLTFLMKFYGMDGFEHLKNQGYVPQTVPE
                     LSTEKDMFAQIREKDILFHHPYQTFQPIVDFVGLAAKDPDVLAIKQTLYRVSGNSPII
                     AALAQAAENGKQVSVLVELKARFDEENNIVWAKMLEKAGCHVIYGLVGLKTHCKITLV
                     VRRETDGIRRYVHLGTGNYNDATAKLYTDIGLLTCSEPIGEDATAVFNMLSGYSEPVS
                     WNRLVLAPLWLKDRFLELIAREKNNAVQNKPARIIAKVNSLCDADIIEALYEAAMNGV
                     KVDLIVRGICCLKVGIEGISENISVRSIVGNFLEHSRVFYFENAGRSEIYCGSADWMP
                     RNLERRVEIMFPVEEERLKAKVYHIL"
     gene            complement(1962..>2587)
                     /locus_tag="PLU43_05755"
     CDS             complement(1962..>2587)
                     /locus_tag="PLU43_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOO27950.1"
                     /translation="LISNKHDFENDIIACAGIMSKRYKGNKDRNQLLERVAVELFDRT
                     KKMHGMGRRERLLLRIAALLNDCGKYMSLEAAAECGYSIIMATEMIGLSHAEREIVAN
                     VVKFNKTHFEYYEELATATLLERESYLKIAKLTAIFRVADGICRSYRTKVRDVKLRQK
                     GDELIITVDTDEDITLEQGFFSRKAAFFEEVFSIRPVLKYKKKLSAV"
BASE COUNT          645 a          703 c          487 g          752 t
ORIGIN      
        1 tcaagaatat gatatacctt tgccttcaga cgttcctctt cgaccggaaa catgatctca
       61 acacgccgct ccagattccg cggcatccag tctgcggaac cgcagtagat ctcggatcgc
      121 cctgcatttt caaaataaaa gacacggctg tgttcaagaa aatttccgac aatggaacgt
      181 acgctgatat tttcgctgat tccctcaatt ccaaccttca ggcagcagat gccccttacg
      241 atcagatcca ccttcacacc attcatggcc gcttcataca atgcctcaat aatatcggca
      301 tcacagaggg aatttacttt ggcaataatt cttgcaggtt tattttgaac cgcattgttc
      361 ttttctcttg cgatcagctc caaaaaacgg tctttcagcc acagaggcgc cagcacgagc
      421 cggttccaag aaacaggttc ggaatatccg gaaagcatgt tgaacactgc cgtcgcatct
      481 tctccgatcg gctcggaaca ggtcagcagg ccaatatcag tatacagctt tgcagtagca
      541 tcattgtaat ttccggttcc cagatgtaca tatctgcgga taccgtcagt ctcccgtctg
      601 acaacaagtg tgatcttgca gtgcgtcttt aatccgacaa gtccgtaaat aacatggcag
      661 cccgcttttt caagcatttt ggcccatacg atattattct cctcgtcaaa tctggctttc
      721 agttccacaa gtacggatac ctgttttccg ttttcggccg cctgcgcaag cgctgcaatg
      781 atcggagaat ttccgctgac acgatagagt gtctgtttga tggccagcac atccggatcc
      841 tttgctgcaa gccccacaaa atcgacgatc ggctgaaatg tctggtatgg atggtgaaat
      901 agaatatcct tttccctgat ctgcgcaaac atgtcctttt ccgtggaaag ctccggcacg
      961 gtctgcggga catatccctg atttttcaga tgttcaaacc cgtccattcc ataaaacttc
     1021 ataagaaagg tcagatcaag cggtccgttg atcctgtaaa tttcttcatt cctgatatcc
     1081 agttccttac gcagtgtttt aagaagtttt ttatcaatgt cgtctgcgac ctccagccgg
     1141 atcacttcac cccactgccg tcttttcaac tgacgttcga tctcttttaa cagatccgcc
     1201 gcttcgtctt catcgatcgt taaatccgca tttctcataa tacgaaacgg atgtgcacaa
     1261 ataatatcat agttcaaaaa cagcttatcc atattgcgtt cgatgatctc ttccagtaaa
     1321 ataaacgtct tctgtccttc ttttcccggg agctttacaa tacgcggaag cactgccggt
     1381 acctgcacag ttgcaaattc aggctcttcc ctgcctcccc tgctcttctt actgcccttt
     1441 ttggaaagaa gtgcgccgat attcagtgat ttattcctaa tcaggggaaa cggtctggaa
     1501 gaatccactg ccatcggggt cagcaccgga tacacatttt cttcaaaata tcgatccgca
     1561 tacgctgcct gttcatccgt cagcttctca tgtaccggta caaaccggat acccttctgt
     1621 gaaagaagcg gaagcaggga acgattgaat gtggaatact gcagacctac aagttcatgt
     1681 gtcttttcat tcagcttatc cagctgctgt tccggtgtca gtccggcaat atcggtttta
     1741 tggtattccg cattcaccat atccttcagt gatgcaacgc gtatcataaa aaactcatcc
     1801 agattggacg ccgtaatact tagaaatttt aatctttcaa ataacagatt tgttttgtcc
     1861 cgtgcttcgc cgagaaccct ctcattaaac agaagccagc tcaattccct gttataataa
     1921 tattccggat tttttaaatt gattttttcc accgcaacct cctataccgc actcagcttc
     1981 tttttgtatt tcaacaccgg cctgatactg aaaacttctt caaaaaacgc tgccttcctc
     2041 gaaaaaaatc cctgttcgag cgtaatatct tcatccgtat cgaccgtaat gatcagttca
     2101 tcgcccttct gcctcagttt cacatcccgc accttcgtac ggtagcttct gcatattccg
     2161 tccgccaccc gaaagatcgc cgtcagcttg gcgattttta aataggactc ccgctcgagc
     2221 agagtcgccg tcgccagttc ttcgtaatac tcaaaatggg ttttattgaa tttaacgaca
     2281 tttgccacga tctccctctc cgcatgggaa agtccgatca tctccgttgc cataataata
     2341 ctgtagccgc actctgccgc agcttcaaga ctcatatatt ttccgcagtc attcagcagg
     2401 gctgctatgc gaagcagaag ccgctcacgc ctgcccatgc catgcatctt ctttgtacgg
     2461 tcaaacaatt caacggctac tctttcaagc agctgatttc tgtctttatt tcctttatac
     2521 cgcttgctca taataccggc acaggcgata atatcattct caaaatcatg tttattgctg
     2581 atcagct
//
LOCUS       DAOSYE010000282         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30965_length_2577_cov_2.718477, whole genome shotgun sequence.
ACCESSION   DAOSYE010000282 DAOSYE010000000
VERSION     DAOSYE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30965_length_2577_cov_2.718477"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLU43_05760"
     CDS             complement(<1..978)
                     /locus_tag="PLU43_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27951.1"
                     /translation="MQNIVNNKEQLNELPDALPVFCKIINLNGSQESGLEISEELVDQ
                     LQTSSQVADLCVTNPLMAGVGEFEEKDWQTKLDIKVTAANTVSAISGLSQDLLTMEDG
                     TTAEFLQTDRYACIVSEDLLKEKNWSVGDVIPLNLYSYVHTNKYQVNFEPLQLTEYEI
                     AGTMDRFVSTSGQSGRTPEIIIPFETVRELYHQKGIAFTADSASFYVADPLDLNEFKE
                     EMQGYGLLSKAPDALYSYQGNTLLVRDSSFISTASKLRQGFDILTGFLPAVVLTVIFI
                     GYIISILLTDSRQTEYALMRIQGMRAGLCFLTFFLEEFLIVLFGCVISGG"
     gene            complement(1232..>2577)
                     /locus_tag="PLU43_05765"
     CDS             complement(1232..>2577)
                     /locus_tag="PLU43_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO27952.1"
                     /translation="YLFEGGMHWLTGSSSKTSFYKLWCEVGALNDEVRIWNRDPFFIF
                     EHDGHTVCLYRDIEKLQAHFLETAPEDGKEIRKFCRDIKKFMRVQMPVTDIKGVKTKN
                     KANMSFSEGVRMLPALLRMGFYAKQTAGEYAGRFKNPVLRMLLQNITGPDSCATGLLF
                     TIATLASGDGGYPEGGSLAMAARMAAYFKSLGGKLEFGKTVSKVIVEKGAACGVIVNG
                     EQIPADDVIVTQDARRAIDTLFDSPINEAWAVRMRQETVPMLDTFICLGIEADLSDLP
                     ESLLFTTKEPLVCGGIAETTVSFNNYATYQGYAPEGCTAVTSIIGGDTYDFWKACREN
                     GTYEEEKKKLADAYIRILTKRIPQIAGKIAVWNIATPLTYERYLGSFKGSWMTVMKKG
                     SANKGYPLKPERISHLYFAGHRMMPPGGLPVAVETGRKAVQYLCRDTDTVFQGNF"
BASE COUNT          683 a          682 c          491 g          721 t
ORIGIN      
        1 acctccgctt atgacacatc caaaaagaac gatcaaaaac tcctccagaa aaaaggtcag
       61 aaaacacagt cctgcacgca tgccctgtat ccgcatcagg gcatactctg tctgcctgct
      121 gtctgtcagc aggatactga ttatataacc aataaagatc accgtcagca caactgccgg
      181 taaaaagccg gtaagaatat caaatccctg ccgcagttta cttgccgtgg aaataaagga
      241 agaatccctg acaagcagcg tattcccctg ataggaatat agggcatccg gtgctttgct
      301 cagcaatccg taaccctgca tttcttcttt gaattcattc aggtcaagcg gatctgccac
      361 ataaaaggag gccgaatcag ctgtgaacgc aatccctttt tgatgataaa gttcccggac
      421 cgtttcaaat ggaatgatga tctccggagt tctccctgac tgaccggacg tactgacaaa
      481 cctgtccatc gttccggcta tttcatactc agtcagctgc agcggttcaa aatttacctg
      541 atatttattc gtatgcacat aagaatacag attcagcggg atcacatcgc ccacgctcca
      601 gttcttttct tttaacaggt cttcgctgac aatacacgca tacctgtctg tctgcagaaa
      661 ctccgctgtg gttccgtctt ccatcgtaag cagatcctgt gacagacccg atatggcaga
      721 caccgtattt gctgccgtga ccttgatatc cagctttgtt tgccagtctt tttcttcaaa
      781 ctcacctaca ccggccatca gcggattggt gacacacaga tccgctacct gggaagaggt
      841 ctgcagctga tcaaccagct cctccgatat ttccagaccg ctttcctgag atccgtttaa
      901 attgataatt ttgcagaata ccggcagtgc gtccggcagc tcgttaagct gctctttatt
      961 attcacaatg ttctgcaaat agatctccag taaggaaacc agcaatatac agatgagaag
     1021 atttagaatg cttcttgctt tatttctgaa aatgccatat ttaacaatat gaaaaataaa
     1081 catcctgata cctcacaaaa tatttttacc tgagaataag tatactacag cttcatgtat
     1141 aaatctagca aataaaggat aatacaccct gtttttataa taaaatagaa aacaccgcat
     1201 ggcatctgct atgcggtgtt gatctctatc tttaaaaatt tccctgaaaa accgtatccg
     1261 tatctctgca taaatactgc acagccttcc gtccggtctc tactgcgaca ggcagacctc
     1321 cgggcggcat catccgatgg ccggcaaaat aaagatgact gatcctttcc ggtttcaaag
     1381 gatatccttt gttcgcgctc ccttttttca tcacagtcat ccacgatcct ttgaaggaac
     1441 ctaaataccg ttcgtatgtc agcggcgttg caatattcca tactgcgatc ttacctgcga
     1501 tctgcggaat tcgctttgtc aggattctga tatatgcatc tgccagcttt ttcttttctt
     1561 cttcataggt tccattttcc ctgcaggctt tccaaaagtc atatgtatct ccgccgatta
     1621 tagatgtaac agcagtacat ccttccggcg catatccctg atacgtcgca taattattga
     1681 agctgactgt tgtttccgct attcctccgc agacaagcgg ctctttcgtt gtaaacagca
     1741 gactttccgg cagatcagac aggtccgctt caataccaag acagataaac gtatcaagca
     1801 tcggcactgt ttcctgcctc attctgactg cccatgcttc attgatcggt gaatcaaaca
     1861 gcgtatcaat ggcgcgccgt gcatcctggg taacaattac gtcgtctgcc ggtatttgtt
     1921 ccccatttac gataactccg caggctgctc ctttttcaac gatcaccttg cttactgttt
     1981 ttccaaattc cagcttcccg ccaagcgatt taaaatatgc tgccatacgg gcagccattg
     2041 caagggaacc gccctccgga taaccgccgt ctccggatgc aagcgttgcg atcgtaaaca
     2101 acaatcctgt agcacagcta tcaggtcccg taatgttttg cagcagcatc cttaaaaccg
     2161 gatttttaaa acgcccggca tattctcctg ctgtctgttt tgcataaaat cccatgcgca
     2221 gcagcgcagg cagcatacgg acgccctcgg aaaaggacat atttgcttta ttttttgttt
     2281 ttacaccttt gatatccgtt accggcatct gaacacgcat aaatttttta atatcgcggc
     2341 agaacttcct tatttccttc ccgtcctccg gtgctgtttc aagaaaatgt gcctgcagct
     2401 tttcaatatc ccggtaaaga catacggtat gtccgtcatg ctcaaatatg aagaaaggat
     2461 cgcggttcca gatcctgacc tcatcattca gcgcccccac ttcacaccag agtttataaa
     2521 aggatgtttt cgatgaggaa ccggtcagcc agtgcatacc gccttcaaac aaatacc
//
LOCUS       DAOSYE010000283         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_30996_length_2575_cov_3.154762, whole genome shotgun sequence.
ACCESSION   DAOSYE010000283 DAOSYE010000000
VERSION     DAOSYE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30996_length_2575_cov_3.154762"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..2326
                     /locus_tag="PLU43_05770"
     CDS             233..2326
                     /locus_tag="PLU43_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOO27953.1"
                     /translation="MKSNVPEIFGSMVFNDAIMKDKLPKDIYKSLRKTIENGKDLDIT
                     VANVVAIAMKDWAIEKGATHYTHWFQPMTGITAEKHDSFISPQNDGRIIMEFSGKELI
                     KGEPDASSFPSGGLRATFEARGYTAWDPTSYAFVKDGILCIPTAFCSYGGEALDKKTP
                     LLRSMEALSEQAVRVLRLFGDTDVKRVITTVGPEQEYFLVDQKLYEERKDLIYCGRTL
                     FGAMAPKGQELEDHYFGAIKPRISAFMKELNIELWKLGIFAKTEHNEVSPAQHELAPI
                     FTTTNIATDHNQLAMETMKNVAKKHGLVCLLHEKPFDGVNGSGKHNNWSISTDTGANL
                     LEPGKSPHENAQFLLFLAAVIKAVDEYQDLMRVSVASAANDHRLGANEAPPAIVSMFV
                     GDDLAAILDAIENGTSYNGPGSPTMEIGVQVLPSFPKDTTDRNRTSPFAFTGNKFEFR
                     SLGSSQSISGPNIVLNTIVADALKQFADELTGAADFTDALNALIKRTIKEHNRIIFNG
                     DGYSEAWKVEAEKRGLLDLKTTVDALPTFLAEKNVKLFTSHKIFTETEMHSRYEIQLE
                     TYSKILNIEGLTMVDIAKKQILPAVNMYIADLSSTAATKKDLDSSLSCKMELSLVEKL
                     SLLEDAAYEEAETLTAILADAAKYSDGLELATYYRDKVLPSMEALRKSCDEMEVNTAS
                     DVWPFPQYGDLLFMI"
BASE COUNT          776 a          547 c          561 g          691 t
ORIGIN      
        1 aatatcattt ttgaaaaaca attggataat tgccaacgtt tacttgacac aaactttttt
       61 taaaaaagta ttgacaatta ttttctgaaa ggttacaatt cgttacatga aaagcaaagg
      121 cgctatgagt ttcctcacag cgtcttttct attttctgac gtaagctggt tgcttttcaa
      181 cagaggcgga cgtacggggt taaaaaacaa atagaaaagg agatgcatat caatgaaaag
      241 taatgttcca gaaatttttg gaagtatggt attcaacgat gccattatga aagacaagct
      301 tccaaaggat atttacaaat ccctccgtaa aacaattgaa aacggcaaag atttggatat
      361 cactgtagca aacgttgttg ccattgccat gaaggactgg gcaatcgaga aaggcgctac
      421 acactacaca cattggttcc agcctatgac cggtatcaca gcggagaagc acgacagctt
      481 catttcaccg cagaacgatg gccgtatcat tatggaattt tcaggcaagg agctgatcaa
      541 gggagaaccc gacgcatcaa gcttcccttc aggcggttta agagccacct tcgaagcaag
      601 aggttataca gcatgggatc caacctccta tgcatttgta aaagacggta ttctttgtat
      661 tccaacagct ttctgctctt acggcggaga agcgcttgat aaaaagacac cgctgctgcg
      721 ttccatggaa gcgcttagcg aacaggctgt cagagtattg cgtttattcg gcgatacgga
      781 cgtcaaaaga gtcatcacta cagttggtcc tgaacaggaa tatttccttg tagaccagaa
      841 gctgtatgag gaaagaaaag atctgatcta ttgcggacgt actcttttcg gtgccatggc
      901 tcctaaagga caggaattgg aagatcatta tttcggcgct atcaagccga ggatctctgc
      961 ctttatgaaa gaattaaata tcgaactctg gaaactcggt atttttgcaa agacggaaca
     1021 taacgaggtt tcccctgcac agcatgagct tgctccgatc tttacaacaa caaatattgc
     1081 aactgaccac aatcagctgg caatggaaac tatgaaaaac gtagcaaaga aacatggtct
     1141 tgtctgcctg cttcatgaaa agccatttga tggtgtaaac ggaagcggaa agcataacaa
     1201 ctggtccatt tccacggaca cgggagcaaa tcttttagag cccgggaaat caccgcatga
     1261 aaatgcacag ttcctgcttt tccttgcagc agtgatcaaa gcagttgacg aatatcagga
     1321 tctgatgaga gtttccgttg catctgcagc aaatgaccac agacttggtg caaacgaagc
     1381 gcctcctgcc attgtttcca tgttcgtggg cgatgacctt gcagcgatcc tggatgcgat
     1441 cgaaaatggt acttcctata atggacctgg ctctcctaca atggagatcg gcgttcaggt
     1501 gcttccttcc ttcccgaagg atacgacaga ccgaaacagg acttcaccat ttgcctttac
     1561 aggaaacaaa ttcgagttca gaagccttgg ttcttcacag tccatttccg gaccgaacat
     1621 cgtattgaat acgatcgtgg cagatgccct caaacagttt gcagacgagc ttacaggtgc
     1681 tgctgatttc acagacgcac tgaacgcact gatcaaacgt acgatcaaag aacataaccg
     1741 tattatcttc aatggtgacg gttattcgga agcctggaag gttgaagctg aaaaacgcgg
     1801 tcttcttgat ctgaagacaa cggtagacgc actgcctact ttccttgctg aaaagaatgt
     1861 caagctgttc acaagccaca agatcttcac ggaaacagaa atgcattcca gatatgagat
     1921 ccagcttgaa acatacagca agatattgaa catcgaagga ctgacaatgg ttgatatcgc
     1981 taaaaagcag atacttcccg ctgttaatat gtacattgcc gatctgtcgt ctacggcagc
     2041 aacaaagaaa gaccttgact cttctctttc ctgtaaaatg gaattaagtc tggttgagaa
     2101 gctttccctg ttagaagacg cagcttatga agaagcagaa acacttactg caattcttgc
     2161 agacgcggct aaatattccg acggactgga actcgctact tattacaggg ataaagtact
     2221 tccttccatg gaagcactcc gtaagtcctg tgatgaaatg gaagtcaata ctgcttcgga
     2281 cgtatggcct ttcccgcagt atggcgatct gttgtttatg atttaatagc atcttcgtat
     2341 ttttatttca gttttatata cacgatgaag tgtaccgtaa gacggtttct tacagtgcac
     2401 ttcattttta ttgctttttg gtttattaaa aaaaaacatt aaaatacata aaatgaggag
     2461 gacaatatta tggcaacatt atttttatct tcggttactc cggagagtgt cgacacaatt
     2521 tggctattgc tcggtgcagc acttgtattc tttatgcagg caggctttgc aatgg
//
LOCUS       DAOSYE010000284         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31095_length_2570_cov_3.453678, whole genome shotgun sequence.
ACCESSION   DAOSYE010000284 DAOSYE010000000
VERSION     DAOSYE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31095_length_2570_cov_3.453678"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>347)
                     /locus_tag="PLU43_05775"
     CDS             complement(<1..>347)
                     /locus_tag="PLU43_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO27954.1"
                     /translation="VIKIGDEQYGIDIKYVENIVRMQKITRVPKVQKYFKGVINLRGE
                     VVPVMSLRLKMNLAEDETMNKTRIIIIKLEANAPVGIIVDEVKEVVTLDDSCIEVVSR
                     KAKSEEVSYINGI"
     assembly_gap    348..357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(409..2508)
                     /locus_tag="PLU43_05780"
     CDS             complement(409..2508)
                     /locus_tag="PLU43_05780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOO27955.1"
                     /translation="MDVSQYLEIFLDETNEHLQSLSDQLMILENEPENEDTINEIFRA
                     AHSLKGMAGTMGYKRMQALTHDMENVLSEIRNGKMKVSSHLIDILFQALDALEAYKNQ
                     IQETRDEGTNDNEPIIQALQKILDEGLNGPKDNEPAAEEKKAETAEETPVTAGEDKWN
                     DIELHEPEQHVIREAIGKGMHVYGITVFVQETCILKAARAFLVFKALEELGETIVSEP
                     SVQDIEDEKFDFDFSVYFVTKESYEKVTAAIMNVSEIDHAVGEEIGLNSIKLSKSEES
                     LTEQTYTVKTEVPADKGKAAPQPSSAAPSASKKAQTGKPVVNRTVRVDIEKLDVLMNL
                     VSELIIAKNSLVSFNAGDGTNANFNEQIEYLESVTTNLHESVMKVRMVPIESVVNKFP
                     RMIRDLSKKLNKKMELFMSGEETELDRTVVDEIGDPLMHLLRNSADHGLESEDVRRAR
                     GKDITGSIFLDAYQDGNNVVIEVRDDGNGISVDVVKAKAIERGTISPEQAESMSDKDI
                     IDLLFLPSFSTAKVVSDVSGRGVGLDVVKSKIEALGGDVSVKTKLGLGSTFTIRLPLT
                     LAIIQALMVVVGGEKYAISLGSIQTIEDIAEDEVKYVEAKEVINLRGNVIPLVRLHTI
                     LGVESTKDSSENMVVVIVKKGEALAGLVVDELIGQQEIVIKSLGKYINCTSRMISGAT
                     ILGDGEVALILDANVLI"
BASE COUNT          619 a          687 c          441 g          813 t
ORIGIN      
        1 cgattccgtt aatataggaa acctcttcgg actttgcttt tctggagact acctcaatac
       61 agctgtcatc cagtgtcaca acctctttga cttcgtcaac gataataccg accggcgcgt
      121 tcgcttccag tttaatgata atgatcctcg tcttgttcat cgtttcatct tccgcaagat
      181 tcatcttaag acgcaggctc ataaccggta cgacttctcc tcttaaatta ataacaccct
      241 taaaatattt ctgcactttc ggtactctcg taattttctg catacgaaca atattctcaa
      301 catatttaat atcgatccca tactgttcat cgccgatttt aataacannn nnnnnnnccg
      361 tattatattc tgccgtattt tttattccgt ccatctctta tttccccctt aaatcagcac
      421 atttgcatcc agaatcaatg caacttcgcc gtcaccgagt atcgtagctc cgctgatcat
      481 acggctcgta caattgatat acttgccgag tgacttaata acgatctcct gctgtccgat
      541 cagttcatct actacaagac ctgccagcgc ttcgcccttt ttcacaatga caactaccat
      601 gttttccgaa gaatcttttg tggattccac accaagtatc gtatgaagac gtacaagcgg
      661 aatcacattg cctcttaaat tgattacctc ttttgcttcc acatacttta cttcatcttc
      721 cgcaatatct tcgatcgtct gaatggagcc taaagaaatc gcatacttct cgccgcctac
      781 aacaaccatc agcgcctgaa tgatcgccag tgtcagcggc agacggatcg taaaggtcga
      841 accaaggcca agctttgtct ttacgctgac atcaccgccc aatgcttcta ttttggattt
      901 tacaacatcc agtccgacac ctctgccgga aacatcggat acgacctttg ccgtagaaaa
      961 actcggaaga aacagcagat caataatatc cttgtcgctc atgctctctg cctgttccgg
     1021 tgaaatcgta ccgcgctcga ttgcttttgc cttgacaacg tcaacactga taccgttacc
     1081 atcgtctctg acttcgatca ctacattatt gccgtcctga tatgcatcaa ggaaaataga
     1141 accggtaata tccttgcccc tggctcttct gacatcctct gattccagtc catgatcggc
     1201 agaatttctt aacagatgca tcaacggatc gcctatttca tctactacgg tcctgtcaag
     1261 ctctgtctct tcacctgaca taaacaattc cattttttta ttcagcttct ttgacagatc
     1321 cctgatcatc ctcgggaact tattgacaac gctttctatc ggaaccattc ttactttcat
     1381 gacagactcg tgaaggtttg tcgtaacact ctccagatat tcgatctgtt cattaaaatt
     1441 tgcattggtc ccgtctcctg cattgaaaga gacaagagag ttcttggcaa tgatcagctc
     1501 acttacaaga ttcataagta catcgagttt ttcaatatcc accctgacgg tcctgttaac
     1561 aacaggcttt ccggtctgtg cttttttgga ggcagacggt gctgcagaag acggctgcgg
     1621 tgctgctttc cccttgtcag ccggtacttc tgtttttacg gtatatgtct gttctgtcaa
     1681 agactcctcg gatttactca gcttgatact gtttaatccg atctcttcgc cgaccgcatg
     1741 atcgatttcg gaaacattca taatggccgc agttactttt tcataagact cttttgtcac
     1801 aaaataaaca ctgaaatcaa aatcaaattt ttcatcttca atatcctgta cggacggttc
     1861 ggatacgatc gtctctccca gctcctcaag tgccttgaac acaagaaatg ctctggctgc
     1921 ctttaaaata caggtctcct gcacaaaaac agtaattcca tatacatgca tgccttttcc
     1981 gatcgcttcc ctgatcacat gctgctccgg ttcatgaagc tcaatatcgt tccatttgtc
     2041 ttctcccgca gttaccggag tttcttctgc tgtctcggct tttttctctt ctgctgccgg
     2101 ttcattgtct ttcgggccgt ttaatccttc gtccagtatt ttctgcaatg cctgaatgat
     2161 cggctcattg tcgttcgttc cttcatccct ggtctcctgg atctgattct tataagcttc
     2221 cagggcatcc agggcctgaa acaaaatgtc aatcagatgg ctgctgactt tcatcttgcc
     2281 gttacgtatt tcggaaagca cgttttccat atcatgggtc agtgcctgca tccgcttata
     2341 tcccattgtt cccgccatac ctttcaggga atgggccgca cggaaaatct cattgatcgt
     2401 atcttcgttt tccggctcgt tctccaaaat catcagctgg tcggaaagac tttgtaaatg
     2461 ctcatttgtt tcgtcgagaa aaatctctaa atattggctt acatccatag tcagtgtacc
     2521 cccacattct taataatctg ttccgctact ttttcaagcg aaacgatctc
//
LOCUS       DAOSYE010000285         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31234_length_2563_cov_2.691388, whole genome shotgun sequence.
ACCESSION   DAOSYE010000285 DAOSYE010000000
VERSION     DAOSYE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31234_length_2563_cov_2.691388"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLU43_05785"
     CDS             complement(<1..724)
                     /locus_tag="PLU43_05785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOO27956.1"
                     /translation="MSTERTLDPGKKKEFEKIISSGYHNITGIAVQKDGRCVYEKYYQ
                     GYTATDTVHVASVTKSIFSALIGIAVAQGAIRSIDDKVLDFFPEHTVLQGEKSAQDVT
                     IKNMLTMTAPYKFKTEPYEAFFASENWMDAALDLLGGTGRAGKFQYSPIAGTHILSGI
                     LVKATGQPVFDFAAFHLFSPLGITVGQNVILHSKEEQLAFFQGKTVSGWVADPQGINP
                     AGWGLTLTPADMAKIGQLYLDNG"
     gene            complement(766..1695)
                     /locus_tag="PLU43_05790"
     CDS             complement(766..1695)
                     /locus_tag="PLU43_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOO27957.1"
                     /translation="MADQKCLEEYIRRIHRVQDYIEGHLDCPMSVEELSNVAGFSKYH
                     FSRIFQGMLQESLAHYVNRVRMEGAVFMLAHRADKNMTDIAYALGFTDSAVFSRTFKN
                     FYGVSPSKYRKEYRKNCKEVFLLSSYNKDTAEKKRKTDLFPVAGQIMIEHMEEKQAVY
                     VRHTGTYETLAEEYAGLIRTLFVMAQKQHFYVDEKYRPFAIYHDNPEFGEKSQFRTSL
                     CLPVPKNASVKEDGILGKMKLESGPYAVGYFRIRQEQYADAWNYMYREWLTGSGYVPG
                     NSAPFEVYCNNPHTDVNHIHEVKICVPIEPIRF"
     gene            complement(1761..>2563)
                     /locus_tag="PLU43_05795"
     CDS             complement(1761..>2563)
                     /locus_tag="PLU43_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27958.1"
                     /translation="MKEMFMILKATVQKEITMFIRYLPNMIGTILQLGVRFLFFFYLS
                     GITAYNGSITGKNLFLFFGAALLLYVIYSTTIWAPLNTVTTDLYNGTLEYIYVTPVSK
                     FCYYFGTGISQTIMSMVFFVPIFAALVLYSGVGFVQVGGIFLVAAAGTLNCIAIGVLL
                     SLLGIIWKQVGAITGIIAVLFEFLAGAYAPVQTYSIPVKAAAFMLPFTWGYDLARYYL
                     MGEKWTLLLPEPVEWCIYLGMAVLYFAAALLLMERIQNRMLRNGLYAI"
BASE COUNT          704 a          671 c          470 g          718 t
ORIGIN      
        1 ttccgttatc cagatacagc tgccctatct ttgccatatc tgcaggcgtc agggtaagtc
       61 cccaccctgc cgggttgatt ccctgcggat cagcgaccca gccactgaca gtctttccct
      121 gaaaaaaagc aagttgttct tctttgctgt gcagtataac attttgtccg actgtgatcc
      181 caagcggtga aaacaaatga aacgccgcaa aatcaaacac cggctgcccg gttgccttta
      241 caagaatacc ggataaaata tgcgttccgg cgatcggtga atactgaaat tttcccgccc
      301 ggcccgtacc tccaagcaga tcaagcgcag catccatcca attttcactt gcaaaaaatg
      361 cttcatatgg ctctgtctta aatttatatg gtgccgtcat tgtcagcatg ttttttattg
      421 taacgtcctg tgctgatttt tccccctgca aaactgtatg ttccggaaaa aaatccagca
      481 ccttatcgtc aatactcctg atcgctccct gcgctactgc gataccgatc agcgcagaaa
      541 aaatgctttt tgtcaccgat gccacatgga ccgtgtctgt agcagtataa ccctgataat
      601 acttttcata tacgcacctg ccgtcctttt gaacagcgat accggtaata ttatgataac
      661 cgctgcttat tattttttca aactcctttt tctttcctgg atcaagtgtt cgttctgtac
      721 tcatacaaaa tacctccgtt tctctctgca ttgattttca gcatatcaga aacgaatagg
      781 ttctatcggt acacagatct tcacctcatg gatatggttc acatccgtat gcggattatt
      841 gcagtacacc tcaaacggcg cgctgtttcc cggaacataa ccactgcctg taagccattc
      901 tcggtacatg taattccatg cgtctgcgta ctgttcctgc ctgatccgga aatatccgac
      961 cgcatacgga ccactctcca gcttcatctt tccaagaatg ccgtcctcct ttacagatgc
     1021 attctttggc accggcagac agaggcttgt ccgaaactga cttttctctc caaattccgg
     1081 gttgtcatga tatatggcaa atggccggta cttctcatcc acataaaaat gctgcttctg
     1141 cgccatgaca aacaatgtgc gtatcaggcc ggcatactct tctgccagcg tctcataagt
     1201 ccctgtatgc ctgacataaa cagcctgttt ttcttccata tgctcgatca tgatctgtcc
     1261 ggccactgga aacaggtctg tttttcgttt cttttctgct gtatccttat tgtacgaaga
     1321 caataaaaaa acttctttgc aattcttgcg atattccttc cgatatttac tcggactgac
     1381 gccatagaaa tttttaaatg tcctggaaaa tacggcagaa tccgtaaaac caagtgcata
     1441 ggcaatatcc gtcatatttt tatctgctct atgtgccagc ataaatactg cgccttccat
     1501 tctaactcgg tttacataat gcgcaagtga ttcctgcagc attccctgga aaattctgct
     1561 gaaatgatat ttggaaaaac cggcaacatt ggaaagctcc tcaacagaca tcggacagtc
     1621 cagatgacct tcaatataat cctgtaccct gtggatcctt cgtatatatt cttccagaca
     1681 tttttgatct gccatatgaa tacctcctgc ctctgtaaaa tccagtatag cagaattact
     1741 tttttttcgc gatcatagtt ttaaatcgca tacagaccgt tacgcagcat cctgttctgg
     1801 atccgctcca tcagcagcag cgccgctgca aaatacagca cggccattcc aagataaata
     1861 caccattcta caggctccgg caaaagaagc gtccactttt ctcccatcag ataatatctt
     1921 gcaagatcat atccccatgt aaacggaagc ataaatgcgg ccgcttttac cggaatactg
     1981 tatgtctgca caggtgcata ggctcccgcc aaaaattcaa acaggaccgc tatgataccg
     2041 gtgatcgcgc cgacctgctt ccagataata cccaacaggg aaagcaggac tcctattgca
     2101 atacagttca atgttccggc ggctgccact aaaaaaattc cgccgacctg tacaaatccg
     2161 actccgctgt acaatacaag cgccgcaaag atcggaacaa aaaataccat gctcatgatc
     2221 gtctgagaaa ttccggtccc aaaataataa cagaacttag agaccggtgt tacgtaaata
     2281 tattccagtg tcccattgta cagatccgtc gtaaccgtat tcagaggcgc ccatatcgtt
     2341 gtcgaataga tcacatacag cagcagtgct gccccgaaaa agagaaaaag attttttccg
     2401 gtaatgcttc cattataagc tgttattccg gaaagataga aaaaaaacaa aaaccggacg
     2461 ccaagctgca ggatcgtacc gatcatattg ggcagatacc tgataaacat agtgatctcc
     2521 ttttgtaccg ttgcttttaa aatcataaac atttccttca ttt
//
LOCUS       DAOSYE010000286         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31263_length_2562_cov_2.794974, whole genome shotgun sequence.
ACCESSION   DAOSYE010000286 DAOSYE010000000
VERSION     DAOSYE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31263_length_2562_cov_2.794974"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..955)
                     /locus_tag="PLU43_05800"
     CDS             complement(245..955)
                     /locus_tag="PLU43_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOO27959.1"
                     /translation="MRGDTAMWPAFMAYIRELNLLSIVIRLLLALITGLVIGVDRGMK
                     RRGAGIKTHVLVCLGSALAMMTGQYILNYFGSGNDVGRIGAQVVSGVGFLGVGTIIVT
                     GRNQVRGLTTAAGLWACACTGLAIGIGFYEGALIMLFLVVITLAGLAKLDLYVFHHSK
                     VLELYIEVDSIRQNAELLRLLREMDLRVASYETMKSKVKNDFVSVMATLELPDRAGRE
                     DTLMKIRSVEGVVFVEEL"
     gene            complement(1207..>2562)
                     /locus_tag="PLU43_05805"
     CDS             complement(1207..>2562)
                     /locus_tag="PLU43_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO27960.1"
                     /translation="IPIIQVIAKDGKEIENMTQAYTEASGTMINSGDWNGMESAVLKK
                     EAPEMIEKRGFGKKTVNYKLRDWVFSRQRYWGEPIPIVHCPDCGCVPVPEEQLPLLLP
                     NVESYQPTGTGESPLADITDWVNTTCPVCGKPAKRETNTMPQWAGSSWYFLRYIDNHN
                     QKELVDREKADAMLPVDMYIGGVEHAVLHLLYSRFYTKFLHDIGVIDFDEPFTKLFNQ
                     GMITGKNGIKMSKSKGNVVSPDDLVRDYGCDSLRLYELFVGPPELDSEWDDRGIDGVY
                     RFLTKFWNLVLDNCDRGVKANAQMIKYRNKMIYDITYRLENFSLNTVISGFMEYNNKF
                     IEMSKNGGIDRATLETFIVLLAPFAPHISEELWHRMGHQASVFKEVWPKADEEAMKDT
                     EKEVAVQVNGKTRVVIKIAADADRETAVAAGKAALGDRLTGTIVKEIYVPGRIINVVC
                     K"
BASE COUNT          654 a          688 c          480 g          740 t
ORIGIN      
        1 tccactgctt catcgcctgt ctgtgtaaaa aaatactgtg cggaatcatc atgaagcatt
       61 atttttatac gattcagtaa agaaacatca tcaagaacaa ataaaaccgt acgcatagtt
      121 gattccttcc ttatctcctt atattttgat atcactaaga aggaatacat acgtattcct
      181 tcttcatttt actatatcta gacaaaattt caagtctttt tgccatatgt tgatattaaa
      241 agcattataa ctcctctaca aacacgaccc cttccacact gcggatcttc attaaagtat
      301 cttctctacc ggctctgtca ggaagttcca gtgtcgccat cactgataca aagtcatttt
      361 tgaccttgga tttcattgtt tcatagctcg ccacccgcag atccatttca cgcagcagtc
      421 taagcaattc ggcattctgt ctgatgctgt ccacctcaat ataaagctcc aaaacttttg
      481 agtgatgaaa cacatataaa tccagttttg caagtcctgc cagtgtaata actacaagaa
      541 agagcatgat cagcgcgcct tcataaaaac cgataccgat cgcaagtccg gtacacgcac
      601 acgcccacaa tccggctgct gttgtaagtc ctcttacctg gttccttccg gtcacgatga
      661 tcgtgccgac cccgagaaaa ccaactccgc tgacgacctg tgcccctata cggccaacat
      721 catttccgga accaaaataa tttaaaatgt actgcccggt catcatggca agcgcagagc
      781 caagacatac aagcacatgc gtcttgatcc cggcaccccg ccgtttcatc cccctgtcaa
      841 cgccgatcac caagcctgta ataagcgcaa gcagcagtct gattacaatg gaaagcaaat
      901 tcagttcacg tatatatgcc ataaacgcag gccacatggc agtatcccct ctcatgataa
      961 tcctgttttc tatattatac tgtttttgtt tccaatccgc aaccatttac ggcagtgccc
     1021 caagctgcga gaccttcgtc tctcgcttga cgggcattcg ccctataagg cttcagacaa
     1081 tacggcagtg ccccaagctg cgagacttcc gtctctcgct tgacgggcat tcgccctata
     1141 aatacgacca gaaggagtct ctttcctgca agcaggagag gctccttctg atcgtatttg
     1201 cactgcctat ttgcagacaa cgtttatgat tcggccgggt acgtagattt ctttgacgat
     1261 tgtaccggtc agcctgtcgc caagtgctgc ctttccggct gccacggctg tttccctgtc
     1321 ggcatctgcc gctattttta taaccaccct tgtctttccg ttgacctgta ccgcaacttc
     1381 tttttccgta tccttcattg cttcttcgtc ggcttttggc catacttctt tgaatacgga
     1441 tgcctgatgc cccattctgt gccaaagttc ttcactgata tgcggggcaa acggtgccag
     1501 taaaacaata aatgtctcaa gtgttgccct gtcaatgcct ccgtttttcg acatttcaat
     1561 aaatttattg ttatactcca taaatccgga aatcacagta tttaaactga aattctccag
     1621 acggtatgta atatcataga tcatcttatt gcggtatttg atcatctgtg catttgcctt
     1681 tacgcccctg tcacagttat caagcaccag gttccagaat ttcgtcagga agcgataaac
     1741 accgtcgatc cctctgtcat cccactcaga atccagttcc ggcgggccga caaataattc
     1801 ataaagcctt agcgaatcac agccataatc acgtacaagg tcatccggag aaacaacgtt
     1861 gccttttgac ttgctcattt tgatcccgtt ctttcctgtg atcatgccct gattgaacag
     1921 ctttgtaaat ggctcatcaa aatcaatcac tccgatatca tgcagaaact ttgtataaaa
     1981 tctggaataa agcaaatgca gcaccgcatg ctccacacca ccgatataca tatcaacagg
     2041 aagcattgcg tctgcctttt cgcggtcaac aagttccttc tgattgtgat tgtcgatata
     2101 tcttaagaaa taccatgaag aacccgccca ctgaggcatt gtatttgttt cccgttttgc
     2161 aggtttcccg cataccggac atgtcgtgtt cacccagtct gtaatatcgg caagcggtga
     2221 ttcacccgta ccggtcggct gataggattc tacatttgga agcagcagcg gaagctgttc
     2281 ctccggaacc ggtacacagc cgcagtccgg acaatgtacg atcggaatgg gttctcccca
     2341 gtaacgctgt ctggagaaca cccagtctct cagcttgtaa ttaactgttt tcttaccaaa
     2401 gccgcgtttt tcgatcattt ccggtgcttc ttttttcagg acagccgact ccatgccgtt
     2461 ccagtctcct gaattgatca ttgtgccgga agcttccgta taagcctgtg tcatgttttc
     2521 aatttctttg ccgtcttttg caatgacctg aatgatcgga at
//
LOCUS       DAOSYE010000287         2557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31339_length_2557_cov_3.388090, whole genome shotgun sequence.
ACCESSION   DAOSYE010000287 DAOSYE010000000
VERSION     DAOSYE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31339_length_2557_cov_3.388090"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLU43_05810"
     CDS             complement(<1..745)
                     /locus_tag="PLU43_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOO27961.1"
                     /translation="MAESMAGLKRTHRCTELDEKNIGETVTVMGWVQKSRNKGGIIFV
                     DLRDRSGILQIIFEESDVTAEGFAKAEKLRSEFVIAVVGKVERRAGEANTNLATGAIE
                     ICAKELRILSEAETPPFPIEENSRTKEELRLKYRYLDLRRPDLQKNIILRSRIVSMIR
                     NYFAGEGFLEIETPILMKSTPEGARDYLVPSRVHPGMFYALPQSPQLFKQLLMCSGYD
                     RYFQVAKCFRDEDLRADRQPEFTQIDLEMS"
     gene            complement(783..2051)
                     /gene="hisS"
                     /locus_tag="PLU43_05815"
     CDS             complement(783..2051)
                     /gene="hisS"
                     /locus_tag="PLU43_05815"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO27962.1"
                     /translation="MALKKKPVTGMKDILPQEMQIRDYVTGLIKETYGGFGFQSIETP
                     CVEHIENLCSKQGGENEKLIFKILKRGEKLDLKSAEKEDDLVDSGLRYDLTVPLSRYY
                     ANHANELPSPFKALQMGSVWRADRPQKGRFRQFVQCDIDILGDPGYLAEIELILATTT
                     LLGKLSFKNFTVRINDRRILKAMASYSGFPKDRYDLVFIILDKMDKIGSSGVREELLS
                     NDFSQDSVDTYMELFEKITHDISGIVYLRETLGDFLPADTAENLETIIAGVNDTKVSE
                     FKIEFDPTLVRGMSYYTGTIFEISMDEFGSSVAGGGRYDEMIGKFTGVQTCACGFSIG
                     FERIITILLEKDFQVPSSGEKMALLIEKGAGSEDVCMVIKEAAKLRAEGRQVLVAQMK
                     KNKKFQKEQLIKEGYALENIKEFYHTELKD"
     gene            complement(2054..>2557)
                     /locus_tag="PLU43_05820"
     CDS             complement(2054..>2557)
                     /locus_tag="PLU43_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOO27963.1"
                     /translation="LILGLPGEDLPMVMHTLEEIRALCPDSMTVHSMAIKRAAGMARF
                     LSEHEDIRSVNTPEMMEAAADAAKSMGMKPYYLYRQKNMAGNFENTGYAKEGKYGIYN
                     ILIMEEIQSIAALGAGSISKVVFDDGRIERTDNVKDVTMFMQESDKILEKKEKFYGKL
                     RNNKRGE"
BASE COUNT          602 a          727 c          461 g          767 t
ORIGIN      
        1 atgacatttc cagatcgatc tgcgtgaatt ccggctgtct gtccgctcgc aggtcttcat
       61 cccgaaaaca ctttgctacc tgaaaataac ggtcgtaacc ggaacacatc aaaagctgct
      121 tgaacagctg cggcgactgc ggcagcgcat aaaacatgcc gggatgcaca cggctcggca
      181 caagataatc ccttgctccc tccggtgtac ttttcataag gatcggcgtt tcgatttcca
      241 gaaatccttc ccctgcaaaa taatttctga tcatgctgac gatcctgctt cttaaaataa
      301 tattcttctg cagatccggt cttcttaaat ccagatacct gtattttaaa cgaagttctt
      361 cctttgtcct gctgttttcc tcgatcggaa acggcggcgt ctctgcctca gataaaatac
      421 gcagctcctt tgcgcagatc tcgatcgcac cggtcgctaa attcgtattg gcctccccgg
      481 cccgcctttc cactttcccg actacggcaa ttacaaattc acttcgcagc ttttcagcct
      541 tcgcaaatcc ttctgccgta acatcacttt cttcaaaaat gatctgcaaa ataccggaac
      601 ggtctcttaa atccacaaaa ataataccgc ccttgttcct gctcttctgt acccagccca
      661 taaccgttac cgtttcaccg atatttttct catcaagctc cgtacacctg tgcgttctct
      721 tcagtcccgc cattgattct gccatcatgt caccgtttcc tctttccgtt atatgattct
      781 gcttagtcct ttaactccgt atgataaaac tctttaatat tttccagggc atagccttct
      841 ttgatcagct gctccttctg aaacttttta ttttttttca tctgggccac aagcacctgc
      901 cggccctcgg cacgcagttt tgccgcctcc ttaatgacca tacagacatc ttcgctgcct
      961 gctccctttt cgatcagcag cgccatcttt tctccgctgc tcggcacctg aaagtctttt
     1021 tccagaagga tcgtgataat gcgctcaaac ccgatggaaa aaccacaggc acacgtctgt
     1081 acccctgtaa atttaccgat catctcatca tatctgccgc cgccggcaac actgctgccg
     1141 aactcatcca tgctgatctc aaagatcgta cccgtatagt aagacatgcc gcggacaagt
     1201 gtcggatcga attcaatttt aaattcggat acctttgtgt cattgacacc cgcaatgatc
     1261 gtttccagat tttctgccgt atccgcaggc agaaagtctc caagcgtttc ccttaaataa
     1321 acaataccgg aaatatcatg tgttattttt tcaaacagtt ccatgtatgt atcaacggaa
     1381 tcctgcgaaa aatcatttga cagcagttct tccctgacac cgctgctccc gatcttatcc
     1441 attttgtcca atatgatgaa cacaaggtca tacctgtctt ttggaaaacc gctgtaagaa
     1501 gccattgctt ttaaaatcct gcggtcattg atacgcaccg taaaattttt aaaggaaagc
     1561 tttccaagaa gcgttgttgt ggccagaatc agctcgatct cggccagata ccccggatct
     1621 cccaagatat cgatatcaca ctgcacaaac tgtctgaaac gtcccttctg cggtctgtcc
     1681 gcacgccaca cgcttcccat ctgcagcgcc ttaaaaggag acggcagttc atttgcatga
     1741 ttcgcataat atctggaaag agggactgtc agatcgtagc gcaggccgga atctaccaga
     1801 tcgtcttctt tttccgccga tttcagatcc agcttttcac ctctttttaa tattttaaag
     1861 atcagttttt cattttcccc gccctgcttg ctgcatagat tctcaatatg ctccacacag
     1921 ggagtttcga tcgactgaaa gccgaaacct ccgtaagttt ctttgatcag accggtcaca
     1981 tagtccctga tctgcatttc ctgcggaagg atgtccttca tacctgttac cggctttttt
     2041 ttcaatgcca tactcattct cctcttttgt tatttcttag tttaccataa aatttttctt
     2101 ttttctccag tatcttgtca ctttcctgca tgaacattgt cacatctttt acattatcgg
     2161 tccgctcgat cctgccgtcg tcaaaaacga ccttggatat cgagcccgca ccaagagcgg
     2221 caatcgactg gatctcctcc atgatcagta tattataaat accgtacttc ccttcttttg
     2281 cataaccggt attttcaaaa tttcccgcca tatttttctg acggtacaga taatacggct
     2341 tcatgcccat ggattttgcc gcatctgcgg ctgcttccat catttccggt gtattgacgc
     2401 ttctgatatc ctcatgttca ctcagaaacc gcgccatccc ggccgcgcgt ttgatcgcca
     2461 tggaatgtac ggtcatactg tccgggcaca gagctctgat ctcttccagc gtatgcatga
     2521 ccataggcag atcctctccc ggaagaccga gtatcag
//
LOCUS       DAOSYE010000288         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31360_length_2556_cov_2.980408, whole genome shotgun sequence.
ACCESSION   DAOSYE010000288 DAOSYE010000000
VERSION     DAOSYE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31360_length_2556_cov_2.980408"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLU43_05825"
     CDS             complement(<1..822)
                     /locus_tag="PLU43_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO27964.1"
                     /translation="MIRLLARIFIKDNAQTSNAAVRQSYGVLCGAVGIGLNILLFAMK
                     LFAGFISGSVAVVADAFNNLSDAGSSAIMMVGFRMSGQKPDPAHPFGHGRIEYVTGLI
                     VSIVIILMGIELTKTSFEKILHPTEIVFAAPTIIILIVSVLIKLYMYSYNKKISEKIS
                     SSAMQATALDSFTDSAATSIVLFSMMVAEFTGVHIDGYCGLLVAAFILYTGFVSAKDT
                     LDPLLGTKPDKAYSGKIEQFVLSYPNVLGVHDLIIHDYGPGRMMISLHAEVPASGD"
     gene            complement(878..1606)
                     /gene="truA"
                     /locus_tag="PLU43_05830"
     CDS             complement(878..1606)
                     /gene="truA"
                     /locus_tag="PLU43_05830"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO27965.1"
                     /translation="MILQYEGTRYQGWQKQTATANTIQGRLEELLSKMTGEQIKLNGS
                     GRTDAGVHAEHQVANFCTKSTMTTEEMFSYINRYLPLDIRVISMDEADLRFHSRLHAK
                     EKVYLYRIHNDKVADVFTRRYTYQYENPLDIKAMEQAAELLCGTHDFKAFCTNKRLKK
                     STVRTVFYISIEKKDREIRITYHANGFLYHMVRIMTGTLIEVGNGSRTPESIPQLFNS
                     KDREKAGMLVPGQGLTLLDVRYES"
     gene            complement(1590..>2556)
                     /locus_tag="PLU43_05835"
     CDS             complement(1590..>2556)
                     /locus_tag="PLU43_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO27966.1"
                     /translation="VIGFVTTMPELWETTGKTLKEVTMNFDRYFKKMPVDLQNWLNGI
                     GENIGEYMTNWFQNSGTSAAESASNFAKNLPLAIIGTIMFILASYLFLAEKDYVVKVY
                     QKVFPAKFQERWNLVYKTMKTAVGGYFKAQFKIMGVVCVILFIGFVIVDVKYAFLIAL
                     LIALLDFLPFFGTGTVMWPWAVIAFFQNDYKLAIGLIITWAISQFVRQLIQPKMLGDS
                     IGLEPIPTLVLLYLGFRVGGALGLIIAVPVGMIVINLYKAGVFSNFVYSIQILAKDLS
                     KLRKFDEADLQKEGIPTIEKVEEDIEEARRESREPHEEHPDDPAV"
BASE COUNT          692 a          682 c          488 g          694 t
ORIGIN      
        1 atcaccgctt gccggaactt ccgcatgcaa agagatcatc atacggcccg gaccatagtc
       61 atgaataatc aggtcatgga ctccaagcac attcggatag gacaggacaa actgctcaat
      121 ttttccggaa tacgctttat ccggctttgt gccaagcagc ggatcaagcg tatcctttgc
      181 cgaaacgaat cccgtataca aaataaatgc cgctaccagt aatccgcaat atccgtcaat
      241 atgaacccct gtaaactcag cgaccatcat ggaaaacagt acgatcgacg ttgcggcgga
      301 atcggtaaag ctgtccagag ccgtagcctg catggctgaa gaactgatct tctcggaaat
      361 ctttttattg taagaataca tatagagttt tatcagcacg gacacgatca gtataataat
      421 tgtcggcgct gcaaaaacga tctcagtagg atgcagtatt ttctcaaaag aggtctttgt
      481 aagttcaatt cccattaaaa tgataacaat ggaaacgatc agacccgtta cgtattcgat
      541 cctgccatgt ccgaacggat gagccggatc gggcttttgc ccggacatcc gaaaacctac
      601 catcataatt gctgaggaac cggcatcgga aagattattg aatgcatcgg caacaactgc
      661 tacggaaccg ctgataaacc cggcaaacag cttcatggca aacagcagga tattcagccc
      721 gatgccaact gctccgcaaa gcacaccata tgactgtctg acggcagcat tcgatgtctg
      781 cgcattgtcc ttaataaata tcctcgcaag taatctaatc attttccgac cttcgtttct
      841 tcctttttta tatacttatc aaagctctct gaccgcctca ggattcatat ctgacatcca
      901 gcagcgtcaa gccctgccca ggcacaagca ttcctgcttt ttccctgtct tttgaattaa
      961 acagctgcgg aatgctctca ggtgttctgc tgccgtttcc gacctcgatc agcgttccgg
     1021 tcataatacg taccatatga tataaaaacc catttgcatg ataagtaatt ctgatctccc
     1081 tgtctttttt ttctattgaa atataaaata ctgtccggac agtcgatttt ttcagacgtt
     1141 tatttgtaca gaacgccttg aaatcatgcg tcccgcaaag cagttctgca gcctgctcca
     1201 tagcttttat atcaagcgga ttctcatact gataagtata ccgccttgtg aacacatcgg
     1261 caaccttatc attgtggatc cggtaaagat ataccttttc ctttgcatgc agccggctgt
     1321 gaaaccgcag atcggcctca tccatggaaa taacacggat atccaaaggc agataccgat
     1381 taatatagct gaacatctcc tctgtcgtca ttgtgctttt tgtacaaaaa ttggcgacct
     1441 gatgctctgc atgcacacct gcatccgttc tgccggagcc attgagtttg atctgctccc
     1501 ctgtcatttt cgaaagcagc tcctccagcc ttccctgtat cgtattggcc gttgccgtct
     1561 gtttctgcca tccctgatac cgcgttcctt catactgcag gatcatccgg atgttcctca
     1621 tgcggttctc tgctttctct tcgagcctcc tcaatatctt cctccacttt ctctatcgta
     1681 ggaattcctt ccttttgcag atctgcttca tcaaatttac gcagtttgct cagatcctta
     1741 gccagtatct gtatgctgta aacaaaattg ctgaacacac ccgctttata cagattgatc
     1801 acgatcattc cgacaggcac cgcaatgatc aggccaagtg ccccgccgac gcgaaagccc
     1861 agatacaaaa ggaccagtgt cggtatcggc tcaaggccaa tcgaatcccc gagcatttta
     1921 ggctgtatca gctgtctgac aaactgactg atcgcccatg taatgataag accgatcgcc
     1981 agcttataat cattttgaaa aaaagcaatg accgcccatg gccacatgac tgttccggtt
     2041 ccgaaaaacg gaaggaaatc gagcagggcg atcagcagcg caatcaaaaa cgcatacttt
     2101 acgtctacta tcacaaagcc gataaataaa atcacacaga cgactcccat gatcttaaac
     2161 tgtgccttaa agtatccgcc taccgctgtt ttcatcgttt tataaacaag attccatcgt
     2221 tcctgaaatt tcgccggaaa aactttctga tataccttga caacataatc cttttcggcc
     2281 agaaacaggt aagatgccag tatgaacatg atcgttccga taatcgccaa cggcagattc
     2341 tttgcaaaat tactggcact ctccgcagcc gacgtccctg aattctggaa ccagttcgtc
     2401 atatactctc cgatattttc cccgatccca ttcagccagt tctgcagatc gaccggcatc
     2461 ttcttaaaat aacggtcaaa gttcatcgtg acttctttta aagtctttcc ggtcgtctcc
     2521 cagagctccg gcattgtcgt aacaaatccg atcact
//
LOCUS       DAOSYE010000289         2555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31384_length_2555_cov_2.853200, whole genome shotgun sequence.
ACCESSION   DAOSYE010000289 DAOSYE010000000
VERSION     DAOSYE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31384_length_2555_cov_2.853200"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLU43_05840"
     CDS             <1..398
                     /locus_tag="PLU43_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO27967.1"
                     /translation="AKITVADSEYEGSVSEIKRFAQGATSDKAKVTVAVHIDDPNENV
                     YLGLEADVTIYTEEKDEALTIPAEAYYTDDDGAYCYVIRNGVIAKQYFTAGIVSDEAV
                     EVLSGLTEGDVVVTDAITDEQIGEKAVAE"
     gene            395..1180
                     /locus_tag="PLU43_05845"
     CDS             395..1180
                     /locus_tag="PLU43_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO27968.1"
                     /translation="MKLLKRPQKEKSLKEIKDISEIAAVEEDGVLIEFKNVSKEYRRG
                     ERVFYALKDVNLVINEGEMVVILGPSGAGKSTLLNLLGGMDSASSGEIWFEGKDLTTF
                     SDNQLTEYRARNVGVVFQFYNLIPTLTAYENVALMKDIEEDTLDPKEALEAVGLAEHL
                     HQFPSQMSGGEQQRTSIARAVAKNPKLLLCDEPPGALDTETGRDVLALLQRMSREKKR
                     TVIMVTHNSIFADLANTVIRVKNGTIKEVRKNEKPKEANEVSW"
     gene            1180..>2555
                     /locus_tag="PLU43_05850"
     CDS             1180..>2555
                     /locus_tag="PLU43_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOO27969.1"
                     /translation="MLFRKLWRDLLQNKTQFFSIFLMSVLGMWIFVGFDAEASGAGHA
                     ADNYYKEYNLADLWVQGDVFSSDDLKAIQDIKGVQNAERRFLMNGTAKLENNPYMYIG
                     FLTTNQISKLALMQGEGYVKDKDGIWVEEDFARANRLEIGDTFTVKLNNQEIESVIRG
                     YVESPEYVYYLSDTDMIYPDYSKYGFAFLSDVQYPDQKNILYNQILVDVQDDADVQLI
                     KDEIGEVLDRDDIVVTDRKQNTSYFTFHSEVKQHKAMGILFSVIFLLIALLGIVTTMA
                     RVTANQRTQIGTLKALGFSKHVITMHYISYGFVISLAGCIIGAWAGYSTFPPLILGMF
                     DGVYKVPDLYGTLTVSSVFAILAAVVISTFVSFLACRKELADMPAVTLKPAAPKKVKH
                     TALEKSGFWLRLNFSTQWNVRDIMRNKIRSIMGIVGVTGCAMLMLCAFGCRDAINGMV
                     DKMYGELLT"
BASE COUNT          731 a          463 c          717 g          644 t
ORIGIN      
        1 gtgccaaaat aacggtcgct gatagtgagt atgaaggaag cgtcagtgag atcaagcgtt
       61 ttgcacaggg agcaacatct gataaggcaa aggtcacggt tgcggttcat attgacgacc
      121 cgaacgagaa tgtttatctt ggactggaag cagatgtgac gatctatacg gaggaaaagg
      181 atgaagcgct tacgattcct gccgaagcgt attatacgga tgatgacggg gcctattgtt
      241 atgtgatcag gaacggcgtg atcgccaaac agtattttac ggccggtatc gtctcagatg
      301 aagcggtcga ggttctttcc ggacttacgg aaggagatgt ggtagtcacc gatgcaatta
      361 ccgatgaaca gatcggagag aaagcggtgg cagaatgaaa ttgttgaaga gaccgcaaaa
      421 agaaaaaagc ctgaaggaaa taaaagatat cagtgagatt gctgccgttg aggaagacgg
      481 tgttttaata gagttcaaaa atgtttccaa agaatacaga cgcggagaac gggtctttta
      541 tgcattgaaa gatgtcaatc ttgttataaa cgaaggagaa atggtcgtga tccttgggcc
      601 gtccggtgcc ggaaagagca cgcttttaaa tctgctcgga ggcatggaca gtgcaagcag
      661 cggagagatc tggtttgaag gaaaggatct gacaacgttt tccgataatc agctgacgga
      721 ataccgtgcg agaaatgtcg gcgtcgtatt tcagttttat aacctgatac caacgctgac
      781 tgcatatgaa aatgttgcgc tgatgaaaga tattgaagag gacacgctgg atccgaagga
      841 agcgctcgag gcggtaggac ttgcggagca tctgcatcag tttccgtccc agatgtccgg
      901 cggagagcag cagaggacgt cgatcgcaag ggcagtggca aaaaatccga agctgctttt
      961 gtgtgatgaa ccgccgggtg cgctggatac ggagacaggg cgtgacgttc ttgcgctttt
     1021 gcagcgtatg agccgtgaaa aaaaacgtac ggtgatcatg gtaacgcata atagtatatt
     1081 tgcagatctc gcaaatacgg tcattcgtgt caaaaacggt acgattaaag aagtcaggaa
     1141 aaatgagaaa ccaaaagagg cgaatgaggt gagctggtaa tgctgtttag aaagctatgg
     1201 agagatctac tacaaaataa aacgcagttt ttttctattt ttctgatgtc ggtactggga
     1261 atgtggatat ttgtcggatt tgatgcggaa gcatccggag ccggtcatgc agcggacaat
     1321 tattataaag agtataatct ggctgatttg tgggtgcagg gcgatgtttt ttcatcagat
     1381 gatttaaagg ctatacagga catcaaaggt gtacagaatg cagaacgccg gtttcttatg
     1441 aacggaaccg ccaaattgga aaacaatcca tatatgtata tcggatttct gacgacaaat
     1501 cagatctcaa agcttgcact tatgcaggga gaaggctatg tcaaagacaa agacggcata
     1561 tgggtcgaag aggattttgc aagggcaaac aggctcgaga tcggagatac gtttacggtg
     1621 aagctcaata atcaggagat cgagagtgtg atacggggtt atgtggaatc tccggaatac
     1681 gtctattatc tgtcagatac cgacatgatc tacccggatt actcaaaata cgggtttgca
     1741 ttcctttccg acgtacagta tccggatcag aaaaatatcc tttataacca gatccttgtc
     1801 gatgtgcagg atgatgcgga tgtacagctt ataaaagatg aaatcgggga agtgctcgat
     1861 cgggacgata ttgttgtgac ggacagaaag cagaacacaa gctattttac atttcattcc
     1921 gaggtcaaac agcataaggc aatgggaatt ttattttcgg ttatatttct gctgatcgca
     1981 ctgcttggga tcgtgacaac gatggcaaga gtgactgcca atcagcggac acagatcggt
     2041 acgctgaagg cacttggatt ttcgaagcat gtcattacca tgcattatat ttcttatggc
     2101 tttgtgatca gtcttgcagg ctgtattatc ggagcgtggg ccggttacag tacatttccg
     2161 ccgctgattc tcggaatgtt tgacggcgta tataaggtgc cggatctgta tggaacgctt
     2221 actgtttctt cggtttttgc gattctggct gcggtcgtta tatcgacgtt tgtgagtttc
     2281 ttagcatgcc gaaaggaact ggcggatatg cctgccgtga cactgaaacc ggcggcaccg
     2341 aaaaaggtga agcatacggc acttgaaaaa agcggattct ggctgagact gaatttttcc
     2401 acacagtgga atgtgcgtga tattatgcgc aataagatcc ggagcattat gggaattgtc
     2461 ggtgtgacag gctgtgcgat gctgatgctg tgtgcgtttg gctgccggga tgcgatcaac
     2521 ggcatggtag ataaaatgta tggggaactg ctgac
//
LOCUS       DAOSYE010000290         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31554_length_2547_cov_2.903692, whole genome shotgun sequence.
ACCESSION   DAOSYE010000290 DAOSYE010000000
VERSION     DAOSYE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31554_length_2547_cov_2.903692"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1419
                     /locus_tag="PLU43_05855"
     CDS             382..1419
                     /locus_tag="PLU43_05855"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HOO27970.1"
                     /translation="MSIFKSLRSKLIASISGSFLIVVAMILTICIISLNNYFSAENTT
                     QLNTYTASFNTLLENYKTQALAQGENIAVNPDIIEGVANRDFDFLLEVSAPLMEKGKL
                     DYMVFTDPDGYIILRTHKPDAVPTENDSIAGQINVASAMKGTSCVWIEPGKVVKLSVR
                     AGVQVIQKMLTVASNAHEVAAATEEISASMEEVTKHSVNTNELADHLQERIDTFSLRD
                     DSTRSDAEIIEQVKSDHYIFVVMLGNMLKGLQTVTADSISTDHSCRFGKWYFTADNPY
                     RDYKVYKDIETPHRKLHEYAQKTVSLYEKGNLVGAKAAYNNVQKYSHQVVGILNKLEK
                     STAKANTAHSE"
     gene            complement(1468..2064)
                     /locus_tag="PLU43_05860"
     CDS             complement(1468..2064)
                     /locus_tag="PLU43_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOO27971.1"
                     /translation="MNTYKYILFDVDGVLAKAQNTAAEVLKENNITIEEFFQGWDVSR
                     AVFDFETGSSTPAEFSEARGEELDRGLQPDAVMKILAAQKSVLYNGVEELLDKLYKRK
                     FTLACLSNTNVLHWNSIEGKEIFDRYFFRKFLSHEMGLMKPQKEIYQVVLDTLQCSAR
                     EILYFDDSGRNIEAALELGFQAVRVKDFDDMAKKIESC"
     gene            2202..>2547
                     /locus_tag="PLU43_05865"
     CDS             2202..>2547
                     /locus_tag="PLU43_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27972.1"
                     /translation="MSIHNKKSNPTSRKKIFWSSAALILLLIGILFSTFSKALPYLSE
                     DNSYTADYAVSGEIYSFRKQAYLIEHYNDHGDEFDYTSAAEYLDGANRVISSPDALHK
                     LEAEDGDDVYYLE"
BASE COUNT          781 a          562 c          431 g          773 t
ORIGIN      
        1 gcacaacact gacatgttca acaatatgca cccattcgac caaattaagc aggcctgcct
       61 ttcctgccac tattcttaac tgatactgat ttttcagcgt atgatatact ttttctaatt
      121 ttatggccat gagcattcct ctcctggaat acaaaaacaa aagccgcctg taaagacagc
      181 ttccttgcac tttttcgaat aattttattt ccctatagtt tagtatataa taaactgata
      241 atttattcaa tcccggcatt gttattttac agtaaattta ttactctttc ttttcccata
      301 atttgtatac ttctttaaca acctatgtta ctatgttaat ataaatactt attatcattt
      361 tcatataagg ggatttttct catgtcaata tttaaaagct tacgttctaa actgattgct
      421 tctatttcag gatctttttt aattgtcgtt gccatgatcc tgaccatctg tatcatctcc
      481 ttaaacaatt atttttctgc agaaaatacc acacagctga atacttatac tgcctcgttc
      541 aacacgcttt tagaaaacta taaaacacag gcacttgcac agggtgaaaa cattgctgtg
      601 aatccggaca tcatagaagg ggttgctaat cgggattttg attttctgct cgaagtttcc
      661 gctccgctga tggaaaaggg caaattagat tatatggtat tcactgatcc tgacggttat
      721 atcatattaa ggacacataa gccggacgct gttcctacag aaaatgacag tattgccgga
      781 cagatcaatg tggcctccgc aatgaaagga acttcttgcg tctggatcga acccggcaaa
      841 gttgtaaagc tttctgttcg tgccggcgtt caggtcatcc aaaaaatgct gacagtcgca
      901 tctaatgctc atgaagtggc agctgcaacc gaagagatct cagcctccat ggaagaggtc
      961 acaaaacact ctgtcaatac aaatgaattg gctgatcacc tgcaggaacg cattgacacc
     1021 ttttctctca gggatgattc cacgcgcagt gatgcggaaa taattgaaca ggtcaaatcg
     1081 gaccattata tatttgtcgt tatgcttggc aatatgctga aaggactgca gacagttact
     1141 gcagactcga tttctacgga tcatagctgc agatttggaa aatggtattt tacagccgac
     1201 aatccatatc gtgattataa ggtctataag gatatcgaaa cgccgcacag aaaactgcat
     1261 gaatatgccc aaaaaactgt ttccctttat gaaaaaggca atctggtcgg tgcaaaggcc
     1321 gcatataaca atgttcaaaa atattcacat caagttgtgg gaatactgaa caagcttgaa
     1381 aaaagcacgg caaaggcaaa tacagctcat tccgaatgat aaaattccaa cggctccctg
     1441 gttttaaaat accggggagc cgttttatca gcagctttct attttttttg ccatatcatc
     1501 aaaatccttt acccgtacag cctgaaatcc cagttctaat gcggcctcaa tatttcttcc
     1561 ggaatcatca aaatataaaa tttcacgcgc actgcactgc aaagtatcca gtacgacctg
     1621 atagatctct ttttgcggtt tcatcaggcc catttcatga gataaaaatt tcctgaaaaa
     1681 ataacggtca aaaatttctt ttccttctat actgttccag tgaagaacat ttgtgtttga
     1741 caaacaggct aacgtaaatt tccttttata cagcttatca agcagttctt ccacaccatt
     1801 atataaaaca cttttttgtg ctgcaagtat tttcatgaca gcatccggct gcagacccct
     1861 gtccagttct tcccccctgg cttctgaaaa ttccgccggc gtactgctgc ccgtctcaaa
     1921 atcaaacact gctctggaaa catcccagcc ctgaaaaaat tcttctattg taatattatt
     1981 ttccttcaga acctccgcag cagtattttg tgccttagca aggacaccat ccacatcaaa
     2041 caaaatatat ttataagtat tcatatatac gctcccattg tgttatttgt ctcatccttt
     2101 catcatacag gaaattgtct gcaaattaaa tttgatacaa aacaaaataa aggatatact
     2161 attagtaatt tcaaatattc ggaaacagaa aggaaccggt tatgtcaatt cacaacaaaa
     2221 aatcaaatcc tactagcaga aaaaaaatat tttggagcag tgccgccctt attctccttc
     2281 tgataggaat tttattcagt actttctcaa aggcccttcc ctatctctca gaagacaact
     2341 cctatacagc agattatgcc gtttcaggcg aaatatattc tttcagaaag caggcctatc
     2401 ttatagaaca ttacaacgat catggcgatg agtttgacta tacttctgcc gcagaatacc
     2461 tggatggggc aaaccgcgtt atttcctctc ctgatgcatt acataagctt gaagccgagg
     2521 atggggatga cgtctattac ctggaag
//
LOCUS       DAOSYE010000291         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31586_length_2545_cov_3.590763, whole genome shotgun sequence.
ACCESSION   DAOSYE010000291 DAOSYE010000000
VERSION     DAOSYE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31586_length_2545_cov_3.590763"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLU43_05870"
     CDS             complement(<1..686)
                     /locus_tag="PLU43_05870"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HOO27973.1"
                     /translation="MFRESYEKKLSGNYLLLEEEHEAENNEFEVRMLTENKIPGLLPC
                     SVRLIDKKKQYAYDITSRQSLERLYETQDIGFLDMQILLRGIMSGIESVSEYFLEEGN
                     LILRPAYIYMDIETKQAALCFFPFYQMDIRQSFQEFSEYLLQKTDHADERAVVLAYGF
                     YKQVINEDYCFEKLFQRELPVSETIPVLEAEEKRPEAASSSNAGLQEKKLPGNEKTAF
                     FISCSVLLLL"
     gene            complement(656..1132)
                     /locus_tag="PLU43_05875"
     CDS             complement(656..1132)
                     /locus_tag="PLU43_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOO27974.1"
                     /translation="MQGYGKRNSLQKKKEKQGSFTIELALLMPFLIGSFLFVFFMAYY
                     MHDRCLLEKACQSAVLRGSGCMSEEAAKTAAYETIDEILLKKLLWKWEFTKQVEIDSE
                     KISVFGEGVMLVNEGGNRPLSFSVECSAKRINEPVFIRSQKGQGKGYCSEKAMKKN"
     gene            complement(1144..1653)
                     /locus_tag="PLU43_05880"
     CDS             complement(1144..1653)
                     /locus_tag="PLU43_05880"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO27975.1"
                     /translation="MCFLAFMLLGLCSYTDIKERVVKIKELFCFLIAALLWLVFSFLS
                     REFIRPESYLPETVVRLFQLWEESFFDRPSLLAFITGILPGTGLYICSRLFRGGIGEG
                     DAWLFMLTGLMLGVRVNVLMLMLGLLLSAVYAGYLLLVKKRGRKESFPFIPFLFLAYI
                     ILFTAALRS"
     gene            complement(1668..2441)
                     /locus_tag="PLU43_05885"
     CDS             complement(1668..2441)
                     /locus_tag="PLU43_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27976.1"
                     /translation="MSAAVSLPFNGSLTVEAALVLPLFLFSMIALLSLLSLLLFHVRV
                     QGVLQQQAKYMAQQAYTEAAFDKEDVCLSVLDEIGEYMLSHAPIKGGAEGLDFSETKL
                     SDREFIKLSVSYEAELPYDLFHLFHYQFSQDCLMHTWIGYEKGLEGIGQDKEEEIVYI
                     TEDSEVYHRDRECSYLRLKIKEVSPEEIETLRNSSGEKYKSCELCHGSLTDSLLYITE
                     DGGRYHSSLDCSGLKRTVTAIPISKVGNRRPCSRCGCLE"
BASE COUNT          723 a          658 c          432 g          732 t
ORIGIN      
        1 attaataata ataaaacaga acagctgata aagaaagccg ttttttcatt tcccggcagt
       61 ttcttttcct gtaaacctgc gttagatgag cttgcagctt cgggtctttt ttcctctgct
      121 tcaagaaccg gtattgtttc agaaacaggg agttctcttt gaaacagctt ttcaaaacaa
      181 tagtcctcat tgatcacctg tttataaaag ccataggcaa ggacgaccgc cctttcgtcg
      241 gcatgatctg ttttttgcag caggtattcg gaaaattcct gaaaagactg tctgatatcc
      301 atttgataaa aaggaaaaaa acacagcgca gcttgttttg tttcaatatc catatagata
      361 tatgcaggac gcagtatcag atttccttcc tccaaaaaat actccgacac ggattcgatc
      421 ccgctcataa ttccccgaag caggatctgc atatcaagaa agccaatatc ctgcgtctca
      481 taaagtcttt ccaatgactg tctggacgta atatcatacg catactgttt ctttttatca
      541 attaatctga cagaacacgg cagcagtcct ggaattttat tttccgtcag catccgcact
      601 tcaaattcat tattttccgc ttcatgctct tcttcaagca gcagatagtt cccgcttaat
      661 tttttttcat agctttctct gaacaatacc cttttccctg ccctttttgt gatctgataa
      721 aaacgggttc attgattctc ttcgcactgc attccacgga aaatgataac ggacggtttc
      781 ctccttcatt taccagcata acgccctccc caaaaactga tatcttttcg ctgtcaattt
      841 cgacctgttt cgtgaactcc catttccaaa gtaatttttt caaaagtatt tcgtcaatcg
      901 tctcatatgc agccgttttt gccgcttctt ccgacatgca gccgctgcca cgcaaaacgg
      961 cagactgaca tgctttttcc aaaaggcatc tgtcatgcat ataataagcc ataaaaaaaa
     1021 caaataaaaa cgatcctatg agaaacggca tcagcaatgc cagttctatc gtaaagcttc
     1081 cctgtttttc tttctttttc tgtaaactgt ttcttttccc gtaaccttgc atcctccctc
     1141 ctctcacgaa cgtaaagcag ccgtaaacag tatgatgtat gccaaaaata aaaaaggaat
     1201 aaatgggaag gattccttcc ttcctctttt tttcactaac aggagatagc ctgcataaac
     1261 ggccgataat aaaagtccca gcatcagcat aagaacattg actctgaccc caagcatcag
     1321 accggtaagc ataaacagcc atgcatcgcc ttcgccgatc cctccgcgaa aaagccggct
     1381 gcagatatac aggcctgttc ccggaaggat ccccgttata aatgcaagca gggacggccg
     1441 gtcaaaaaaa ctttcttccc acagctgaaa caggcggaca actgtttccg gtaaataact
     1501 ttccggtctt ataaactctc tgctcaaaaa agaaaaaacc agccacagca atgctgcaat
     1561 caaaaaacag aacaattctt ttattttaac aactctttct tttatatccg tataactgca
     1621 tagtccaagc agcataaacg ccagaaaaca catgtctgtt tctccctcta ttctaaacag
     1681 ccgcaccgcg aacacggacg tctgtttccc acttttgaaa taggaattgc cgtcacagtc
     1741 cttttcagtc cgctgcagtc cagactgcta tggtacctgc ctccgtcttc agtaatatac
     1801 agcagactgt ctgtcagact gccatgacaa agctcgcagc ttttatattt ttcaccggaa
     1861 ctatttctca gtgtttcaat ttcttctggt gaaacttctt ttattttcag ccgaagataa
     1921 ctgcattccc tgtccctgtg atatacttca ctgtcctccg taatataaac gatctcctcc
     1981 tctttatcct gccctattcc ctccagccct ttttcatacc cgatccacgt atgcataaga
     2041 caatcctggc tgaactgata atgaaacaag tgaaaaagat cataaggaag ctctgcttca
     2101 tagctgaccg aaagctttat aaactctctg tctgacagct ttgtttcgga aaagtcaagt
     2161 ccttctgccc cgcctttgat cggggcatgg cttaacatat attctccgat ctcatctaaa
     2221 acagacaggc atacgtcctc tttgtcaaat gctgcttccg tataagcctg ctgcgccata
     2281 tattttgcct gttgctgcag cactccctgc acccggacat gaaacagcag cagactcagt
     2341 aaagacagca gcgcaatcat ggaaaataaa aacaatggca gcaccagtgc tgcttcaact
     2401 gtcagactgc cgttaaaagg cagactgaca gcagcagaca aaaatgctct ctttcgtctg
     2461 ctttgggcct gaacagccct atgtctgcat atccggaaaa aaactttttt gtatgaaccg
     2521 aaagagagca tggtcatccg cctcc
//
LOCUS       DAOSYE010000292         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31772_length_2536_cov_3.274486, whole genome shotgun sequence.
ACCESSION   DAOSYE010000292 DAOSYE010000000
VERSION     DAOSYE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31772_length_2536_cov_3.274486"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..918
                     /locus_tag="PLU43_05890"
     CDS             64..918
                     /locus_tag="PLU43_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27977.1"
                     /translation="MNIFTKRNLKLFFRDRSAVFFSLLAILIIIVLYAVFLGDVWLPD
                     AMEGVNGVDTLMDSWLVAGLLAVASVTTTMGAFGIMIDDRVKKADRDFYASPFPRSRL
                     MAGYLGSAFWIGTIMSIITFAAAEVYIVLRGGSLLEPFLCMKIFLLIFLSTIANTSAV
                     CFIVSFFRSQHAFSTASTIIGTVIGFLAGIYLPIGSLPSSVQTVVKLFPVSYSASLFR
                     SILMTGPMEISFDGAPAQILSDFKIYMGVTYYFGDYEISPFASILILAGTAVIFFCLS
                     LFNLSKKR"
     gene            1098..2057
                     /locus_tag="PLU43_05895"
     CDS             1098..2057
                     /locus_tag="PLU43_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOO27978.1"
                     /translation="MFKNKGLKYNKQWISQRADPYVYRHTDGTYYFTASVPAYDGIVL
                     RKANSLAELETAEEVYIWHKHKTGSMSEHIWAPELHFLRGKWYIYFAASSVEDVWALR
                     PYVLVCTGNDPMTDSWEEMGMMQPADEDEFSFRAFSLDATIFENRGEHYYIWAEKVGV
                     GRQISNLYIAKMESPCKLKTVQVLLTTPDYDWERTGIWVNEGPAVIKHDGKIFVTYSA
                     SDTGNNYCIGMLTIEDDKDLLDPRQWKKERNPVLSSDEGKSIFGPGHNSFTIDEKKRD
                     IMVYHARTEKETTGNPLYNPNRHTMLMEIKWNKENMPVFSFLS"
     gene            2090..>2536
                     /locus_tag="PLU43_05900"
     CDS             2090..>2536
                     /locus_tag="PLU43_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOO27979.1"
                     /translation="MGKHTIAVLFCMAICVISAGCGRQEEVFEETPITEIKKGDSLGV
                     MASVHDPSIIKGEDGLYYIFGTHMSSAKSTDLVHWKSFSDGVSKSNPLFSNLFDDDLT
                     AFQFVGKNSDNWYSVWAPDVIYNEAMQKYVMYFCTSSTYVCSNICMA"
BASE COUNT          726 a          469 c          612 g          729 t
ORIGIN      
        1 agttaacaac gggttatagg ttaccgttat caacctaaat atattatata aggagttaag
       61 acaatgaata tttttacaaa acggaatttg aagctatttt ttcgtgaccg cagcgcagtc
      121 tttttttcgc tgctggcgat tttgattatt attgttctgt atgcggtgtt tttaggagac
      181 gtatggctgc cggatgccat ggaaggagta aacggcgttg atacattgat ggatagctgg
      241 cttgtggcag gccttcttgc ggtggcttcg gttacaacga caatgggcgc gtttgggatc
      301 atgatcgatg acagggtcaa aaaagcagac agggattttt atgcttcgcc atttccaaga
      361 agccgtctta tggcaggata tctgggaagt gccttctgga tcggaacgat catgtcaatc
      421 attacatttg cagctgcaga ggtttatatt gttttaaggg gcggaagtct gctggaacct
      481 ttcctatgca tgaaaatatt tctgcttatt tttttgtcta caatagcaaa tacatctgcc
      541 gtatgcttta tcgtgtcctt ttttaggagc cagcatgcat tcagtacggc aagtacgatc
      601 atcggaacag tgatcggatt tctggcagga atttatcttc cgatcggatc tctgccgtca
      661 tccgtgcaga cagttgtgaa attgtttccg gtttcttatt cggcatccct tttccgcagt
      721 atcctgatga cgggtccaat ggagatttct tttgacggag ccccggcaca gatactaagc
      781 gattttaaga tctatatggg agtaacctat tattttggag attatgagat ttctccattt
      841 gccagcattc tgattctggc aggaaccgcg gtcatctttt tttgcctttc gctctttaat
      901 ctttctaaaa agagataagc ggttattata aaaatccttt cggcagcagt tttgatccga
      961 aaggattttt ttcgtagaaa taaaagctta attgagtctt gcaattacga aaagtatcct
     1021 gtataattta gattacacta aaataattta cttcataata aatgttaaaa aataaaaagt
     1081 aaaagaatga ggaccatatg ttcaagaata aaggattaaa atataataag caatggattt
     1141 cacagagagc cgatccatat gtttaccggc atactgacgg aacctattac tttacggcgt
     1201 ccgtccctgc atatgatggt attgttttaa gaaaagccaa ctctcttgcc gaactggaga
     1261 cggccgaaga agtttatatc tggcataagc ataaaacagg ttccatgagc gagcacatct
     1321 gggcgccgga acttcatttc ctgcgcggga agtggtatat ctactttgcg gcaagcagcg
     1381 tggaagatgt ctgggcactg cgcccctatg ttctcgtctg taccggaaac gatccgatga
     1441 cagactcgtg ggaagaaatg ggtatgatgc agcctgcaga tgaagatgaa ttttcttttc
     1501 gggcattttc actcgatgcg acgatatttg agaacagagg agaacattat tatatctggg
     1561 cggaaaaggt cggtgtcggc agacagatct ccaatttata tattgcaaaa atggagagtc
     1621 cctgtaaatt gaaaaccgta caggtgctgc ttacgacccc tgattatgac tgggaaagaa
     1681 ccggaatctg ggtaaacgaa ggacctgcgg tcattaagca tgacggaaag atctttgtga
     1741 catattcggc gagtgatacc gggaataatt actgcatcgg gatgctgacg attgaagacg
     1801 acaaagatct gctggatccc cggcagtgga agaaggaacg aaatccggtg ctttccagtg
     1861 acgaaggaaa aagcattttc gggcccggcc ataattcttt tacgatagat gaaaagaaac
     1921 gggacattat ggtctatcat gcaagaacgg aaaaagaaac gacagggaat ccgctctata
     1981 atcctaacag acatacgatg ctgatggaga ttaaatggaa taaggaaaat atgcctgtgt
     2041 tttcgtttct ttcttaaaaa tagagaaaaa gctgttaagg gggaagaaaa tgggcaagca
     2101 tacgattgca gttttgttct gtatggcaat ctgtgtcata tctgccggct gcggcagaca
     2161 ggaagaggtt tttgaggaaa caccaattac agagattaaa aaaggggaca gcctgggcgt
     2221 gatggcatcg gttcacgatc cgtcgatcat taaaggggag gatggtcttt attatatttt
     2281 cggaacacac atgtcttcgg cgaaatcgac cgatcttgtt cactggaaat cattttcaga
     2341 cggtgtcagc aaaagtaatc cgctgttcag taacctgttt gacgacgacc tgacagcgtt
     2401 ccaatttgtt ggaaagaaca gtgacaactg gtattcggtc tgggcgccgg atgttattta
     2461 caatgaagca atgcaaaagt atgtgatgta tttctgtaca tcctccacgt atgtctgctc
     2521 aaatatctgt atggcg
//
LOCUS       DAOSYE010000293         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31883_length_2531_cov_2.984249, whole genome shotgun sequence.
ACCESSION   DAOSYE010000293 DAOSYE010000000
VERSION     DAOSYE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31883_length_2531_cov_2.984249"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLU43_05905"
     CDS             complement(<1..326)
                     /locus_tag="PLU43_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO27980.1"
                     /translation="MEEEAVTSKLKKIRKQKKLTLKDMSELTGFSISFLSQMERGISP
                     VTLTSLKKITKALGISMKEIFHEEEASEDSVDIYTRKNPDVRLEGMQRNYKKMTVLSG
                     HFSQRK"
     gene            complement(350..901)
                     /locus_tag="PLU43_05910"
     CDS             complement(350..901)
                     /locus_tag="PLU43_05910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO27981.1"
                     /translation="MNNPEATILMKNGNQIVIELYPQKAPNTVNSFIYAAMHGIFDGH
                     AIERIVPGSWVDFSYSAFGKEEAKYLIPLEFSQIPVEDGYIAMGGYGDLGIAGCEIFF
                     PLRKCPEFNGKYPAFGKVLEGMEELYRLEKTKTVPIDTHPGIEIHKPVEPQIVQNVRL
                     ELYGHLYPPPVRVAHHQLPDSWK"
     gene            complement(898..1974)
                     /locus_tag="PLU43_05915"
     CDS             complement(898..1974)
                     /locus_tag="PLU43_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO27982.1"
                     /translation="MKKERVLKVIDKMKAMGISQLLACDPMSIYYLTGIFQDPGERFY
                     ALLVREEGMPLLFANRLFTIGEQLDMEIVWFSDTDPIMDVVAEKLKQTDRLGVDKNLQ
                     AKFLLPLMEKNAAAGFLNASLVIDEVRSRKDAEEIEKMAAVSKINDLAMEKFKKLVVP
                     GITELAMAHQMLKIYQELGADTYSFAPLVAFGANGAEGHHEPDETVLKEGDSVLIDVG
                     CKKDMYCADMTRTFFYGDVSQKHRELYEIVQCANETAIAKLHPGMKFSDIDAIARNVI
                     TKKGYGANFTHRLGHFIGLEVHEYGDVSSGNEKPAEPGMIFSIEPGIYLPGDVGIRIE
                     DLVLVTETGCKRLNHYNKDLEVIK"
     gene            complement(2001..>2531)
                     /locus_tag="PLU43_05920"
     CDS             complement(2001..>2531)
                     /locus_tag="PLU43_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO27983.1"
                     /translation="GGMIDDIMMRASEMILSFPSLILAMCINAALGSSLMNTMFALVI
                     VAWPSYARLMRSVVITVKENQYIEAASAIGSSKLRIMVHEIIPNSISSIVIMATTDLG
                     NQILMFSTLSFLGLGSSPPTPEWGMMVSDGVDYFNKFWVAGFPGLAIFTMAVGANFIG
                     DGLRDILDPRLRKQIG"
BASE COUNT          656 a          643 c          459 g          773 t
ORIGIN      
        1 attttacgct gactgaaatg cccgcttagg actgtcattt ttttataatt acgctgcata
       61 ccttccagtc tgacatccgg attttttcgt gtatagatat ccacagaatc ttccgaagcc
      121 tcttcttcat gaaaaatctc tttcatggaa atacccagtg ctttggttat tttttttaat
      181 gatgtcagag tcacaggtga aataccgcgt tccatctgcg aaagaaaact gatcgaaaaa
      241 ccggtcagct cactcatatc ttttaatgtc agctttttct gctttctgat ctttttcagc
      301 ttgctggtta ctgcttcttc ttccatatgc ttcgtccctc catcgcagat tatttccatg
      361 aatccggcag ctgatgatgt gctaccctta ccggcggcgg atataaatgt ccataaagct
      421 ccagtctgac gttctgtacg atctgaggtt ctaccggttt atggatctca atgccgggat
      481 gtgtatctat tggcactgtt tttgtttttt ccagtcggta caattcttcc attccttcca
      541 gaacttttcc aaaggccgga tattttccgt taaattccgg acattttctg agcggaaaaa
      601 aaatctcgca gccggcaatt ccaagatcac cgtatccgcc cattgcaata taaccgtctt
      661 ctaccggtat ctgtgaaaat tccaatggaa ttaaatattt tgcttcttct tttccaaaag
      721 ccgaataact gaaatcgacc caggaaccgg gcacgatcct ttcaatcgca tgaccgtcaa
      781 agattccatg catggctgcg tagatgaaac tgttcactgt attcggcgct ttttgggggt
      841 atagctctat tacgatctga ttaccgttct tcattaaaat cgttgcttcc ggattattca
      901 ttttataact tccaaatctt tattatagtg attcagtctt ttacaccctg tttctgtcac
      961 aagaacaaga tcttctatcc tgatgccgac atcccccggc agatagatgc ccggttcaat
     1021 tgaaaatatc attcccggtt ccgcaggctt ttcattcccg gacgacacat ccccatattc
     1081 atgaacctcc agtccaataa aatgtccgag acgatgcgta aaattagctc catatccttt
     1141 tttggtgatc acattcctgg caattgcatc gatatcactg aatttcatgc cagggtgtaa
     1201 cttggcgatc gcggtttcat ttgcgcattg tacgatctca tatagttctc tgtgcttctg
     1261 tgatacatcc ccataaaaaa aggtcctggt catatctgca caatacatat cttttttaca
     1321 tcccacatcg atcaggacac tgtccccttc tttcagtacg gtctcatccg gttcatgatg
     1381 accttccgca ccatttgcgc caaaagcaac gagcggggca aaagaatacg tatctgcacc
     1441 aagctcctga tagatcttaa gcatctgatg cgccattgca agctccgtga tcccgggaac
     1501 gaccagcttt ttaaatttct ccatcgccag atcattgatt tttgagactg ctgccatttt
     1561 ttcaatttct tccgcatcct tgcggcttct cacttcgtct atcacaagtg atgcgtttaa
     1621 aaatccggct gccgcatttt tctccattaa tggaagcagg aattttgcct gcagattttt
     1681 atctacacca agccggtctg tttgtttcag cttttctgcc acaacatcca taatcggatc
     1741 cgtatcggaa aaccagacga tttccatatc aagctgttcc cctatagtga ataaccggtt
     1801 tgcaaataag aggggcattc cctcctcccg cacaagcagc gcataaaacc gttctcccgg
     1861 gtcctgaaat atgcctgtca gataataaat tgacatggga tcgcatgcca gaagctgcga
     1921 aatacccatt gctttcattt tatcaattac ttttaatact ctttcttttt tcataaaaat
     1981 gccgccttat ttaactattt ttatccgatc tgttttctta atctcggatc cagtatgtca
     2041 cgaagaccgt cgccaataaa attagcaccg accgccatag taaagatcgc aagccccgga
     2101 aatcctgcta cccagaactt attaaaataa tccactccgt cagataccat catgccccat
     2161 tcaggcgtcg gcggcgaaga accaagtccg agaaagctca gtgtggaaaa catcagtatt
     2221 tgattcccaa gatccgtagt tgccatgatc acgatagaac tgatactgtt cggtatgatc
     2281 tcgtggacca tgatccgaag cttggaagag cctatggcac tggctgcttc aatatactga
     2341 ttctctttta ccgtaatgac aacactccgc atcagtcttg cataagacgg ccatgccaca
     2401 atgaccagtg caaacatcgt gttcatcagg ctggagccca atgccgcatt aatacacatc
     2461 gccaggatca aagaaggaaa tgaaaggatc atttctgaag cccgcatcat aatatcgtcg
     2521 atcattccgc c
//
LOCUS       DAOSYE010000294         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31890_length_2531_cov_2.542811, whole genome shotgun sequence.
ACCESSION   DAOSYE010000294 DAOSYE010000000
VERSION     DAOSYE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31890_length_2531_cov_2.542811"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLU43_05925"
     CDS             complement(<1..528)
                     /locus_tag="PLU43_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOO27984.1"
                     /translation="MADGKKNPGQLVFGLDIGTRSIVGTVGYTTESVFHVIAQSVKEH
                     ETRAMLDGQIHDIEKVGKTILEVKKNLEEKIGKNLNEVCIAAAGRVLKTITTQTVLDF
                     GGETVVDAEHVYTLEMLGVEKAYEEFQEQNDSDMRFYCVGYTVVKYFLNNYPISTLEH
                     HKAQQKADAAGATLDI"
     gene            complement(559..1158)
                     /gene="coaE"
                     /locus_tag="PLU43_05930"
     CDS             complement(559..1158)
                     /gene="coaE"
                     /locus_tag="PLU43_05930"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256462.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOO27985.1"
                     /translation="MKVIGVTGGIGAGKSEVLKIIEKNCCCHILIADRAAHDVMLSDG
                     SCYHDLIRLLGKEILDARLEIDRRKMAEKIFAEGAWELLKKVNRIVHPAVKKYIVQQI
                     EEKRMKGTVDFFFIEAALLIEDGYAEICDELWYVRAAKEIRIKRLMEMRGYSFEKTKQ
                     IMDSQLPEEVFLQHCKVVIDNNGDLKAVSRQVLELLHRS"
     gene            complement(1179..>2531)
                     /gene="polA"
                     /locus_tag="PLU43_05935"
     CDS             complement(1179..>2531)
                     /gene="polA"
                     /locus_tag="PLU43_05935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOO27986.1"
                     /translation="QIFGKCSMGLAAEEKPQEFLEYACFYSYVCFKTYKALRKQLEET
                     GMYRLFSDIEMPLSVCLYKMETEGILVKPEGLKEYSAELAGQIAELEDAIRQEAGEDF
                     NINSPKQLGEILFVKMGLPGGKKTKTGYSTAADVLEKLSKDYPFVAKILEYRGLAKLK
                     STYADGLVNYIDEKNRIHSTFHQTITATGRISSAEPNLQNIPIRMEQGRLIRKVFVPK
                     DGCLFLDADYSQIELRILAHMSGDEKLIAAYNREEDIHRITASEVFHTPFDEVTPLQR
                     RNAKAVNFGIIYGISSFGLSQDLSISRKEAKEYIERYFETYPQVRRFLDRLVSDAKKD
                     GYAVTMYGRRRPLPELASSNFVQRSFGERVAMNAPIQGTAADIMKIAMIHVQERLERE
                     QLRSRLILQVHDELLIETAVEEQEQVERLLMEEMKGAADLAVKLEVDMHAGMTWYDTK
                     "
BASE COUNT          609 a          690 c          444 g          788 t
ORIGIN      
        1 gatatcgagc gtggcaccag cggcatcagc tttctgctgc gctttatggt gttccagtgt
       61 actgatcgga taattgttca gaaaatattt tactaccgta tagccaacac aataaaatct
      121 catgtcagaa tcgttctgct cctgaaattc ttcatacgcc ttttccactc caagcatctc
      181 cagcgtatat acatgttcag catcaacgac cgtttccccg ccaaaatcca gaaccgtctg
      241 tgtggttatc gttttaagca cacgcccggc ggcagcaata cagacttcat tcaaattctt
      301 gccgattttt tcttcaagat tttttttgac ttccaggatc gtctttccga ccttctcaat
      361 atcatggatt tgtccgtcaa gcattgcgcg tgtttcatgt tcttttacag actgtgcaat
      421 cacatggaat acggactcag ttgtataccc tactgttccg acaatacttc tcgtcccgat
      481 atcaagacca aataccaact gacccggatt cttttttcca tctgccactg ttttttcctc
      541 ctaataccct gcaattcctt atgatctgtg cagcagctct aatacctgac ggctgacagc
      601 cttcagatca ccgttattat ctataacgac cttacagtgc tgcaaaaaaa cttcttccgg
      661 cagctggctg tccatgatct gttttgtctt ttcaaaactg tacccacgca tttccataag
      721 ccgcttgatt cttatttcct ttgctgctct gacataccat agttcatcac aaatttcggc
      781 atacccgtct tcaatcagca gtgcagcttc aataaagaaa aaatccacgg tccctttcat
      841 tcttttttct tctatttgct gtacaatata ttttttcact gccggatgca cgatcctgtt
      901 gacctttttc agcaattccc atgcaccttc cgcaaaaatc ttttccgcca ttttcctgcg
      961 gtcgatttcc agccgcgcat ccagaatttc tttcccaagc agcctgatta gatcatgata
     1021 acaggaaccg tcggacagca tgacatcatg agccgcacga tctgcaatca aaatatggca
     1081 gcagcagttt ttttctataa ttttcagcac ttcggacttt ccggcaccga tcccgccggt
     1141 aactccaatt actttcattt ttatggactc cttatgaatt atttagtgtc ataccatgtc
     1201 atacctgcat gcatatcaac ttcaagctta acggcaaggt ctgccgcgcc tttcatttct
     1261 tccataagca gacgttctac ctgctcctgt tcttccactg ccgtttcgat caaaagctcg
     1321 tcatgcacct gtaagatcag ccgcgatctt aactgctccc tttccaggcg ctcctgcaca
     1381 tgtatcatgg cgatcttcat aatatctgct gccgttccct gaatcggtgc attcattgct
     1441 accctctcgc cgaaagaacg ctgtacaaaa ttgctggagg caagttccgg cagcggtctg
     1501 cgccttccat acatcgtcac tgcatatcca tcctttttgg catctgacac cagccggtca
     1561 agaaaccttc tgacctgcgg atatgtttca aaataacgtt caatatattc ttttgcttct
     1621 ttccttgaaa tactcagatc ctggctgagt ccaaaagaac tgatcccata tatgatccca
     1681 aaattaactg cctttgcgtt tcttctctgc agcggcgtca cttcatcaaa tggcgtatga
     1741 aacacttctg acgctgtgat cctgtgaata tcctcttccc ggttgtacgc ggcgatcagc
     1801 ttttcatcac cggacatatg tgccagaatg cgaagttcga tctgggagta atccgcatcc
     1861 agaaacaaac agccgtcttt tggaacaaat actttacgga tcagacgtcc ctgttccata
     1921 cgaatcggaa tgttctgcag attcggctct gcgctgctga tccgcccggt ggctgttatt
     1981 gtctgatgaa acgtagaatg aatcctgttc ttttcatcga tataattcac aagaccgtcg
     2041 gcataggtgg atttcagttt ggcaagtcct ctgtattcca gtatttttgc aacgaacgga
     2101 tagtcttttg acagcttctc cagcacatcc gctgcagtgg aatatcctgt tttcgttttt
     2161 ttgccgcccg gcagccccat ttttacaaaa aggatctcac caagctgctt cggtgaatta
     2221 atattaaaat cctctcctgc ttcctgacgg atcgcatctt ccagttctgc aatctgccct
     2281 gcaagctctg cgctgtattc ttttaatcct tccggcttta caagaatgcc ttccgtttcc
     2341 attttataaa gacaaacgga aagcggcatt tcaatatccg aaaacagccg gtacattccg
     2401 gtttcctcca gctgctttct caaagcctta tacgtcttaa aacatacgta actgtaaaaa
     2461 catgcatatt ccaaaaattc ctgcggtttt tcctccgcag ccaggcccat ggaacatttt
     2521 ccaaatatct g
//
LOCUS       DAOSYE010000295         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_31970_length_2527_cov_3.456715, whole genome shotgun sequence.
ACCESSION   DAOSYE010000295 DAOSYE010000000
VERSION     DAOSYE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31970_length_2527_cov_3.456715"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLU43_05940"
     CDS             complement(<1..300)
                     /locus_tag="PLU43_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO27987.1"
                     /translation="MSNGNQDNQNRKVQEQDIHQLLKVRREKLANLQAEGKDPFRITK
                     YDVTIHSQDIKDGFEALEGKEVSVAGRMMLKRVMGKASFCNVSDLKGNIQVYVARD"
     gene            complement(343..825)
                     /gene="greA"
                     /locus_tag="PLU43_05945"
     CDS             complement(343..825)
                     /gene="greA"
                     /locus_tag="PLU43_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOO27988.1"
                     /translation="MEEKKNILTYEGLKRLEEELHDLKVVKRAEVAQKIKEAREQGDL
                     SENAEYDAAKDEQRDIEARIDDIEKILKNAEVVLEEEINTDRIGIGCKVKILDKELKQ
                     EMEYKIVGSTEANSLKGKISNESPVGKALLGAKKGETVTVETQAGALKYKVLEIERAN
                     "
     gene            complement(953..1915)
                     /gene="dusB"
                     /locus_tag="PLU43_05950"
     CDS             complement(953..1915)
                     /gene="dusB"
                     /locus_tag="PLU43_05950"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOO27989.1"
                     /translation="MIKIGDVVLPNQWILAPMAGVTDLPFRMLCKEQGAGLLCTEMVS
                     AKAIYYQNKNTEELLEIGKGEAPVSLQLFGRDPQIMSEMAKRIEERPFDILDINMGCP
                     VPKVVNNGEGSALMKEPKLAGEIIERIAKAIKKPVTVKIRKGFDESNCNAAQIAYIAQ
                     ESGAAAVTVHGRTREQYYTGKADWEIIRKVKESISIPVIGNGDVTGYETALKMTTRTG
                     CDGVMIGRAVRGNPWIFRELCAGENRQVYRKPSVDEIRRTVIRHGELLILHKGEHIGM
                     REMRKHAAWYTAGFPHSSRLRGRLNEIETIGQLKTLLYEEWKEN"
     gene            complement(1899..>2527)
                     /locus_tag="PLU43_05955"
     CDS             complement(1899..>2527)
                     /locus_tag="PLU43_05955"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOO27990.1"
                     /translation="SKQGLAVTKIKGVIVASVVPNVMHSLINAIIRYLHCEPLIVGPG
                     IKTGIKIDTPNPKEIGADRIVDIVAAYELYGGPALVLDFGTATTYDLVTEDGCFNVGI
                     TAPGIRISAKALWEDTAKLPEIEIKKPKSILAQDTISSMQAGLVYGQIGQTEYIIRQV
                     RKETGFKNMKVIATGGLGRIIADETEEIDIYNSQLTLEGLRILYDKNR"
BASE COUNT          525 a          730 c          458 g          814 t
ORIGIN      
        1 gtctctggct acatagacct gaatgtttcc cttcagatcg gaaacattgc aaaaggatgc
       61 ctttcccatc acgcgtttaa gcatcatacg tccggcaacg ctgacttctt ttccttccag
      121 cgcctcaaaa ccgtctttga tgtcctggct gtgaatggtc acatcgtatt ttgtgatgcg
      181 aaacggatcc tttccttccg cctgcaggtt tgcaagcttc tctcttctga ccttcagcag
      241 ctggtgaatg tcctgctcct gtactttcct gttctgatta tcctgattac cgttactcaa
      301 ttttattctc ctttattgat caaacccgct gatacctgct gcttaatttg ctcgttcgat
      361 ctccaaaact ttatatttca atgctcccgc ctgtgtctca acagtgacag tctctccctt
      421 tttcgctccg agcagcgctt ttccgaccgg tgactcattg gagatctttc ctttcagact
      481 attggcctct gttgaaccga cgatcttata ttccatttcc tgctttaatt ctttatccag
      541 aattttgacc ttgcagccaa taccgatccg atcggtattg atttcctctt ccagcacgac
      601 ctcagcattt ttcaatatct tttcaatatc gtcgatcctc gcctcaatat ccctctgctc
      661 gtcttttgcc gcgtcatact cagcattttc ggataaatca ccctgttccc ttgcttcctt
      721 gatcttttgg gccacctccg ctcttttcac cactttcagg tcatgaagct cttcctcgag
      781 ccgtttcaga ccttcatagg tcaatatgtt tttcttttct tccattttgc acccttcctc
      841 ttttataaat taactgaatc tatcagctta tttcgatctt tcatctgcca ttaaacaatt
      901 aatggaatta taccgcatac atacttttta tgtcaagatg aatatacctt cctcagtttt
      961 ctttccattc ctcatataac agcgttttca gctgtccgat cgtttcgatc tcattcagcc
     1021 tcccgcgcag tctggaagaa tgagggaatc ccgccgtata ccacgccgca tgttttctca
     1081 tctcacgcat accgatatgt tcgcctttgt gcaggataag cagttctccg tgcctgatca
     1141 ccgtccgcct gatctcgtcc acggaaggct tccgatatac ctgtctgttt tctcctgcgc
     1201 acaattcccg gaagatccac ggattcccgc gtacggcacg tccgatcatc accccgtcac
     1261 atcctgtacg ggtggtcatc ttaagcgcag tctcatatcc ggtcacatcc ccgtttccga
     1321 tcaccgggat cgatatggat tcctttactt tccgtatgat ctcccagtcc gctttgcctg
     1381 tataatactg ctcccttgtc cttccatgaa cagtcactgc cgcagctccg ctctcctgag
     1441 caatatacgc gatctgcgcc gcattgcagt ttgattcatc aaagcctttc cggatcttga
     1501 ctgttacagg ctttttgatc gccttcgcga ttctttcaat aatttctccg gcaagcttcg
     1561 gctccttcat gagcgcagaa ccttctccgt tgtttacgac ctttggaacc gggcatccca
     1621 tgttgatatc cagaatgtca aacggacgct cctcgatccg tttggccatc tcactcatga
     1681 tctgcggatc ccgtccgaaa agctgcaggg aaacaggcgc ttcccctttt ccgatctcca
     1741 aaagttcttc cgtatttttg ttttggtaat agattgcctt ggcgctgacc atctccgtac
     1801 agagcagacc tgctccctgt tccttgcaca gcatgcgaaa tggcaggtcg gttacgcctg
     1861 ccattggtgc cagtatccat tgatttggaa gtaccacatc acctattttt atcatacaat
     1921 atccgtaatc cttctaaagt cagctggctg ttataaatat cgatctcctc tgtctcatcc
     1981 gcaatgatcc ttccaagacc gcctgttgca atgaccttca tatttttaaa cccggtctct
     2041 tttctgacct gcctgataat atattccgtc tgcccgatct gtccgtaaac aaggcccgcc
     2101 tgcatgctcg aaatcgtatc ctgtgccaga atggactttg gctttttgat ttctatttcc
     2161 ggcagctttg cagtatcttc ccataaagct tttgcagaga tccggatccc aggcgctgtg
     2221 attcctacat taaagcatcc gtcttccgta acaagatcat aggttgtcgc tgttccgaaa
     2281 tcaagaacaa gtgccggacc tccgtataat tcataagcag ccacaatatc aacgattctg
     2341 tccgcgccga tctcttttgg attcggcgtg tcgatcttga tcccggtttt gatccccggt
     2401 ccaacaatca gcggttcgca gtgcaggtat ctgataattg catttatcag cgaatgcata
     2461 acattcggaa caacacttgc cacgatcaca cctttgattt ttgtaacggc aagtccctgc
     2521 ttgctga
//
LOCUS       DAOSYE010000296         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32290_length_2513_cov_2.978031, whole genome shotgun sequence.
ACCESSION   DAOSYE010000296 DAOSYE010000000
VERSION     DAOSYE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32290_length_2513_cov_2.978031"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLU43_05960"
     CDS             <1..341
                     /locus_tag="PLU43_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27991.1"
                     /translation="YDYVRRLVSGRYALMLLMSFGAWIFESIVLFGVAGLYAIPFTTE
                     TFSNYISSILSANHSALMRIYTFLSIVLIAVFMIVVSLVILFSKTEKSGKNNCTKADN
                     KKLRKNGDKI"
     assembly_gap    401..410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <411..1759
                     /locus_tag="PLU43_05965"
     CDS             <411..1759
                     /locus_tag="PLU43_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27992.1"
                     /translation="NTIFKRMSLKERIKRRMLQYPYVSQFLEEEAADGGEEPTGKEAG
                     QTIFLLFSDFGLADEQELGVLLEKACYINENYRVLCEGRIAAVIYTSYEQYVRYRDRK
                     SIDNFEKIETEAFIDLSNVNHFRQFITSGFDARFFNVLSGDDYTVVKSSDNSMKLRAE
                     YEFYKLLPDEMKMWFVMPFDFREKEGLASYTMERYHMTDLAIRYVHGAVSQEEFTDIL
                     EKLFCFIRSRKIRQVSNEEYEKAARLLYVDKVDERIAKLKESSRFQTLEALLRSGTIY
                     GGIDGITAKYKELYKKITAGRHFRPVLAIGHGDLCFSNILYNQDASILRLIDPKGAMD
                     EASLYMNPYYDIAKLSHSICGNYDYFNSDLFEITLDEQMRFKLTVDCDNGEYVAAFKE
                     YLEKNELDYKLIRLYEASLFLSMLPLHIDREKKVFAFVLNAVKILEELEEEECIKD"
     gene            1744..2136
                     /locus_tag="PLU43_05970"
     CDS             1744..2136
                     /locus_tag="PLU43_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOO27993.1"
                     /translation="MYKGLSFIFDIDGTLCPIKKKEENYEDLIPYQEMVDKIREYKKD
                     GAKIILFTSRNMNSYQGNIGLINANTAKVVLAWLDKWEIPYDEIIYGKPWPGHKGFYV
                     DDRTVRPDEFLMLSVQELDELCKKSRCD"
     gene            2105..>2513
                     /locus_tag="PLU43_05975"
     CDS             2105..>2513
                     /locus_tag="PLU43_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27994.1"
                     /translation="MNCVKKAGVIDMEIVITMAGLGSRFRKAGYKLPKYMIEACGKTL
                     FEWSMESLSGFYHKDNTYYFIVRKEDDAAGFITEKCKKAGIHQIKLIELNEQTDGQAT
                     TAMLAADDWDKSAPLLIYNIDTYVEAGEMCGGQI"
BASE COUNT          769 a          397 c          620 g          717 t
ORIGIN      
        1 gatatgatta tgtaaggcgc cttgtatcgg gacgttatgc gctgatgctt ttaatgtcat
       61 tcggagcatg gatttttgaa agtatcgttc tttttggggt ggcaggcctt tatgccattc
      121 cgtttactac ggagacattt tccaactata tttcttcgat actttctgca aatcacagtg
      181 cattgatgcg gatctataca tttttgagta tcgtattaat cgcggttttt atgatcgtgg
      241 tatctttggt tattttattt tccaaaaccg aaaaatccgg gaaaaataat tgtacaaagg
      301 ccgataacaa aaaattgcgg aagaatgggg acaaaatatg aaaaaagtga tcgtattcta
      361 tgatgattcc ataaaaccga atcaggatat caaaaacata nnnnnnnnnn gaaatacgat
      421 ctttaaaaga atgtccctta aggaacggat aaaacggcgt atgctgcagt atccgtatgt
      481 atcgcagttt cttgaagaag aggcggccga cggaggggaa gagccgacag gaaaagaggc
      541 cggacaaacg atatttctgc ttttttccga ttttggcctt gcagatgaac aggaactggg
      601 tgttcttctg gaaaaggcat gttatattaa tgaaaattac agggttttgt gtgaaggccg
      661 tattgctgcc gtaatttata catcatatga acagtatgtc agatatcgtg acagaaaatc
      721 cattgataat tttgaaaaga tcgaaacaga agcgtttatt gatttatcaa atgtaaatca
      781 ttttcggcag tttattacaa gtggttttga tgccagattt tttaatgtgc tttcaggcga
      841 cgactatacg gttgtgaaaa gctcagataa tagtatgaaa ttacgtgcgg aatatgaatt
      901 ttacaagctg cttccggatg agatgaagat gtggtttgtt atgccgtttg atttcaggga
      961 aaaggaaggc ctggcgtctt atacgatgga acgttaccat atgacggacc tggcgatccg
     1021 ttacgtgcac ggcgctgttt cacaggagga gtttacggat attcttgaaa agcttttttg
     1081 ttttatccgt tcacggaaga tccggcaggt ttccaatgag gaatatgaaa aagcggcacg
     1141 gctgctctat gttgataagg tcgatgagag aattgcaaaa ctgaaggagt cttcccggtt
     1201 tcagacattg gaagctttgc tcaggagcgg aacgatatat ggcgggattg acggaatcac
     1261 tgcaaaatac aaagagcttt ataaaaagat cactgcaggc agacatttta ggccggtact
     1321 tgcaatcgga cacggtgatc tgtgtttttc taatattctg tataatcagg atgcatcaat
     1381 actgcggctg atcgatccga aaggagcaat ggatgaggcg tctttatata tgaatccata
     1441 ttatgatatt gccaagctgt cacattctat ttgcggaaat tatgattatt ttaatagtga
     1501 tctgtttgag atcacgctgg atgaacagat gcgttttaag ctgacggtcg attgtgacaa
     1561 tggggagtac gttgctgctt tcaaagaata tttagagaaa aatgaacttg attataaatt
     1621 gatcagactg tatgaggcgt cgttattttt atcgatgctg cctctgcata tagacaggga
     1681 gaaaaaagta tttgcatttg tgctgaatgc ggtaaaaata ctggaagagc tggaggaaga
     1741 ggaatgtata aaggattgag ctttatattc gatatcgacg gaacgctctg tcccattaag
     1801 aaaaaagagg aaaactatga ggaccttatt ccttatcagg aaatggttga taagatacgg
     1861 gaatataaaa aagacggggc gaaaatcatt ttatttactt ctcggaatat gaacagttac
     1921 cagggaaata ttggtctgat caatgcgaat acggcaaaag tcgttcttgc ctggctcgac
     1981 aaatgggaaa ttccttatga tgaaatcata tacggaaagc catggccggg tcacaaaggg
     2041 ttttacgtgg atgacagaac tgtccggccg gatgaatttt tgatgctgtc cgtgcaggaa
     2101 ctggatgaat tgtgtaaaaa aagcaggtgt gattgatatg gagatcgtaa ttacaatggc
     2161 gggccttggc tcccgtttca gaaaggcagg atataagctg ccgaaatata tgatagaggc
     2221 atgcggcaaa acattgtttg agtggtcaat ggaaagtctt tcaggatttt accataaaga
     2281 taacacatat tattttattg ttcgaaagga agatgacgct gccggtttta ttacggagaa
     2341 gtgcaaaaag gcagggattc atcagataaa gctgattgaa ctgaacgaac agacagacgg
     2401 gcaggcaacg acagcaatgc tcgctgcaga cgattgggat aaaagcgctc cgctgctgat
     2461 ctataatatc gacacttatg tggaagccgg tgaaatgtgc ggcggacaga tag
//
LOCUS       DAOSYE010000297         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32434_length_2506_cov_3.791922, whole genome shotgun sequence.
ACCESSION   DAOSYE010000297 DAOSYE010000000
VERSION     DAOSYE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32434_length_2506_cov_3.791922"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLU43_05980"
     CDS             <1..321
                     /locus_tag="PLU43_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27995.1"
                     /translation="AHYLKDGLARIAVSDPWIADNHAIFTVVIREGRAVHIETDPIMT
                     GREPGSCDFDIWMPISVFSAAITGNFSLGDFDFMENVTYDETKAELLAGIFYRKDCFI
                     NNYF"
     gene            428..1873
                     /locus_tag="PLU43_05985"
     CDS             428..1873
                     /locus_tag="PLU43_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO27996.1"
                     /translation="MEKVLLFYPPGLMFQRGEDRCQQNIDDGSAQAMRACNDLGYAAA
                     ILLKKGYDVKLRDYQTQKDTMEDLYHDMETYQPDLIMMSITNTTIFDDLKLLGELKNR
                     YHPVVVIKGALFYAPEQAMLDLLDLTNVDYMIGGEVDFAIGGIADYALRKEGAIGDVL
                     SILYKDESGVFRQTAFHTWGEDLDDQPFPARQLMNNALYQRPDTDEPMATIQTARGCP
                     SSCVFCLTPEISGKCVRFRSPENVFAEMTECYEVHGIRNFFFKADTFTINAAWVKKMC
                     ELIIHSPLHGKIEFTANSRVNPLSRETLELMKEAGCFLVAFGFESGSDEILKKMRKGA
                     TVAQNLQAAKWCHEVGLSFWGFFVIGFPWETKAHIMETKKLIMQADPDFIEITIALPF
                     YGTPLYEICKENDLLAESVLGSDFFHSSMTGTMHLSVDELMKLRRNMLLSFYLRPSYI
                     FKKMGQCVVHPGIFINYCKYGIKLVVNLFKH"
     gene            2027..>2506
                     /locus_tag="PLU43_05990"
     CDS             2027..>2506
                     /locus_tag="PLU43_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO27997.1"
                     /translation="MLYQICNGRVAFAAATILENINFEIKNKKEKIAVVGRNGCGKTT
                     LLKLIAGEVGLTRLDGDENVSIVSAGNPTIGYLKQITFEDENQTLDAEIRKVFAGILS
                     IQARLNELVVLMENNAEEQLVAEYTRLQEEFQDIGGYYYEKEYDIMLKNFGFETADKQ
                     "
BASE COUNT          723 a          463 c          645 g          675 t
ORIGIN      
        1 gcacattatt taaaggatgg tcttgcaagg atcgcggtaa gtgatccctg gattgcagat
       61 aatcatgcga tttttacggt cgtgatcaga gaaggccgcg ctgttcatat cgaaactgat
      121 ccgataatga caggcaggga acccgggtcc tgtgattttg acatatggat gccgatttct
      181 gttttttcag cggccattac aggcaatttc tcgcttggag actttgactt catggagaat
      241 gtgacctatg atgagacaaa ggcagagctg cttgcaggaa tcttttacag gaaagactgc
      301 tttattaaca actatttttg acaaacaggg tattcacatg tacaatatag tctgtccgca
      361 gcattttagt atgcatgccg ggtctgttta tatgcaatca ataaagaaga atcgaggaat
      421 ttcatagatg gaaaaagtgt tattgtttta tccgccggga ctgatgtttc aaagagggga
      481 agaccgctgt cagcagaata tcgatgacgg ctctgcgcag gcaatgcgtg cctgcaatga
      541 tctcggttat gcggctgcaa ttcttttaaa aaagggttac gatgttaaac tgcgggatta
      601 tcagactcag aaagatacca tggaagatct gtatcacgat atggagactt atcagccgga
      661 tctgatcatg atgagcatta caaatacaac gatctttgac gatctgaaac tgctcggaga
      721 gctgaaaaac cgttatcatc cggtcgtagt cataaaaggt gcgctttttt atgcgcctga
      781 gcaggcaatg ctggatctgc tcgacctgac aaatgtggat tatatgatcg gcggggaagt
      841 cgattttgca attggtggta ttgccgatta tgcgcttcgc aaggaaggcg cgattggcga
      901 cgtactttct attttatata aggatgaaag cggtgtgttc agacagaccg cattccatac
      961 atggggagag gatctggatg atcagccgtt tcccgcaagg cagcttatga ataatgcgct
     1021 ttatcagagg ccggatacgg atgaaccgat ggcgacgatc cagactgcaa ggggctgtcc
     1081 ctccagttgt gtattttgtc tgactccgga gatctccggt aaatgtgtgc gtttcagaag
     1141 tccggaaaat gtatttgccg aaatgacgga atgttatgaa gtgcatggta tccgtaattt
     1201 cttttttaag gcggatacgt ttacgatcaa tgcggcatgg gtaaagaaaa tgtgtgaact
     1261 gatcattcat tctccgctgc atgggaagat agagtttacc gcgaattcca gagtcaatcc
     1321 gctcagcagg gagacactgg agctgatgaa agaagcgggc tgttttcttg ttgcatttgg
     1381 gtttgaatcc ggttccgatg agatattgaa aaaaatgcgg aaaggtgcga ccgttgcaca
     1441 gaatctgcag gctgcaaaat ggtgccatga agtgggctta agcttctggg gattttttgt
     1501 tattggcttc ccgtgggaaa caaaagcgca tattatggaa acgaagaaat tgatcatgca
     1561 ggcagatcct gattttatcg agatcacgat agcccttcct ttttacggaa caccgttata
     1621 tgaaatatgc aaggagaacg atctgcttgc ggaatccgta ctcggcagtg atttcttcca
     1681 ttcgagcatg acaggtacga tgcatctgtc ggttgacgag ctgatgaagc ttcgcaggaa
     1741 tatgctgctg tcgttttacc tgcgtccgtc ctatattttt aaaaaaatgg gccagtgcgt
     1801 tgtccatccc ggtattttta tcaattattg caaatacggc atcaaactag tcgtaaattt
     1861 gtttaagcat taagccatgc aataaaaagt aaggaatacc atgaggaaag cttgctttca
     1921 cgaacggtat tccttgcttt ttattatacg gcgccagccg cacagcgagg cgccagccga
     1981 gatgtgtcat ggcttaaaga gcgcaggaga gtaagagagg tgtatcatgt tatatcagat
     2041 ttgtaatggc agagtcgcat ttgcagccgc tacaatattg gaaaacatca attttgaaat
     2101 caaaaataaa aaagaaaaaa tagcggtggt cggcagaaac ggatgcggaa agacaacgct
     2161 tttaaaactg attgcaggag aggtcggact cacacggctc gacggggacg agaatgtatc
     2221 gatcgtaagt gcaggaaatc cgacgatcgg ttatttaaaa cagatcacgt tcgaagatga
     2281 aaatcaaaca ctggatgctg aaatcagaaa agtatttgct ggaattttgt ccatacaggc
     2341 acgtctgaat gaactggtcg ttctgatgga aaacaatgcg gaagagcagc ttgttgcgga
     2401 atacacaaga ctgcaggaag agtttcagga tatcggcggt tattattacg aaaaagaata
     2461 tgatattatg ctgaaaaatt tcggatttga gacggctgac aaacag
//
LOCUS       DAOSYE010000298         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32512_length_2503_cov_3.076389, whole genome shotgun sequence.
ACCESSION   DAOSYE010000298 DAOSYE010000000
VERSION     DAOSYE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32512_length_2503_cov_3.076389"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PLU43_05995"
     CDS             <1..1481
                     /locus_tag="PLU43_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO27998.1"
                     /translation="LLHLCITFFTDRQIFTVSPKEGAADYILCKCLAYAALFFFYLAL
                     YHLLIDKNRKEQPITKIIKCALPYLIVLAVVMVIKLPGGYLSNDETLIYNAAVSLTHY
                     TWFYYITTYYYIVSLMLIPNYFGPIMIKILIEFLTVGYFVYRLKRILGNSYGSFGYLL
                     FLLYPVIAYTTSAHRLPIYYLLYLVLITTLLFDYMEKKELHASKALSLLFLGAILTQW
                     RTEGIYLVVLLPILLFLVYKNIRTKKNALAVVFSFLLIQYIVSVPQNGLLPNNMDAAA
                     NDRMKPFYAYTITNMVRNNLNREKNAEDLAIVDQYLSLDTMDAINDYYGDINYEDVLI
                     LYKEGYIGTRPDATIEDFTNYAAALKRIFINNPGVFLKTRWGAFCYAALPYKITFEGL
                     QLRQLFSFAVSIVKTISYNLFIPLSIILLLCIYALVKRRWFTFFTAGGLLAHWFIVFI
                     LAPASYFKYYFPVYIIAYFYVLLLILQLVYNRKQHENLHFIV"
     gene            complement(1562..>2503)
                     /locus_tag="PLU43_06000"
     CDS             complement(1562..>2503)
                     /locus_tag="PLU43_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOO27999.1"
                     /translation="GTGIAATELALLAGAERVEGTLFGNGERTGNIDMLNVAYNMFSQ
                     GIDPKLEIGRINDIIEVYERCCKMPIDDRHPYAGKLVFTAFSGSHQDAINKGVKAMKE
                     RKQEIWQVPYLPIDPADIGRKYEPIVRINSQSGKGGVAFIMDTHFGFKLPKGMHREFA
                     NVIQALSEKHGEISPEEIMAAFKENYIDQKEPLHFRKLKVDDKSDAADTEFDTHIVLT
                     YTYYGKEGSLEAAGNGPIHAVQQGLCERFGIDIRVLDYEEHALQGGSNAQAAAYIHLL
                     DAKTGRAIYGVGISSNITRASVRSIFSAVNRLALLTK"
BASE COUNT          634 a          654 c          473 g          742 t
ORIGIN      
        1 cgctcctgca cctttgcatc acttttttca cggacagaca gatctttacc gtttctccca
       61 aagaaggggc cgcggattat atcctatgca aatgtctggc ctatgcggcg ctctttttct
      121 tttaccttgc gctgtatcat ctgctgatcg ataagaacag aaaagaacag ccgattacca
      181 aaatcataaa atgcgcgctt ccgtatctga tcgtgcttgc cgttgtcatg gtcatcaagc
      241 tgccgggcgg ttacttaagc aatgacgaaa cactgatcta taatgccgcc gtatcattga
      301 cacactatac atggttttat tacattacaa cctattatta tattgtttcc ctgatgctga
      361 taccgaatta cttcggcccg atcatgatca agatcctgat cgaattcctt acggtcggct
      421 attttgtata ccgcttaaaa agaatactcg gcaattctta cggatctttc ggatatctgc
      481 tgtttctgct ttatcctgtc attgcctata cgacaagtgc acaccggctt ccgatctact
      541 atctgcttta tcttgtcctg atcacaacct tattgtttga ttatatggaa aaaaaggaac
      601 tgcacgcttc aaaagcgctt tcccttttat tcctcggtgc aatactgaca caatggcgca
      661 ccgaaggaat ctatctggtc gtactccttc cgatcctgct gttcctcgta tataaaaaca
      721 tccgtacaaa aaagaatgca ctggctgttg ttttctcttt tcttctgatt caatacatcg
      781 tttccgtacc gcagaacggt ttgcttccaa acaacatgga tgccgctgca aatgaccgca
      841 tgaaaccatt ctacgcttat acgatcacaa atatggtccg caataatctc aacagagaaa
      901 aaaacgccga agatcttgct atcgtggatc agtatctttc ccttgataca atggacgcca
      961 tcaacgacta ttacggcgac atcaactatg aggacgtact gatcttatat aaggaaggat
     1021 atatcggcac ccgtcccgac gccacaatag aggactttac aaattatgcg gcagccctga
     1081 agcgtatttt tatcaataat cccggcgtat ttttaaagac ccgctggggt gcattctgct
     1141 atgccgctct tccgtataaa ataacatttg agggcctgca gctccggcag cttttcagct
     1201 ttgccgtcag catcgtaaaa acaatttcct acaatttgtt tattccgctg tccatcatcc
     1261 tgctgctttg tatttacgca cttgtcaaac ggcgctggtt tacctttttt acggcaggcg
     1321 gactgctggc acactggttt atcgtattta tactggctcc cgcctcttat tttaaatatt
     1381 attttccggt atatatcatt gcttacttct atgtactgct gctcatcctg cagctcgtct
     1441 ataaccgaaa gcagcatgaa aacctgcatt ttattgtctg aatacaaaac cggcaaaaca
     1501 gcctgtcact ttatgtgaga gcgttctgcc ggtttcctgt tatttatgta tgattttcct
     1561 gttatttcgt aagtaacgca agcctgttca cagcactgaa aatagaacgg acagacgcac
     1621 gcgtaatatt ggagctgata ccgaccccgt aaattgcccg gccggtcttt gcatcgagca
     1681 gatgaatata ggcagcggcc tgtgcattgg agcctccctg cagcgcatgt tcctcatagt
     1741 caagtaccct gatgtcaata ccaaatctct cacacaaacc ctgctgtacg gcatggatcg
     1801 gtccgtttcc tgccgcttcc agagaacctt cttttccata atacgtataa gtcagtacaa
     1861 tatgcgtatc aaactcagtg tccgcagcat cactcttatc atccactttc agttttctga
     1921 aatggagcgg ttctttctga tcaatataat tttccttaaa agcagccatg atctcttcag
     1981 gagatatttc cccgtgcttt tccgaaagtg cctgaattac attggcaaat tccctgtgca
     2041 tgccctttgg cagcttaaag ccaaaatgtg tatccataat aaaggcaacg ccgcccttgc
     2101 cggactggga attgattctg acgatcggtt catatttcct cccgatatct gccggatcaa
     2161 tcggaagata cggcacctgc cagatctcct gcttccgctc cttcatcgct ttcacacctt
     2221 tattgatagc atcctgatgt gaacccgaaa aggcagtaaa cacaagcttt cccgcatacg
     2281 gatgcctgtc gtcgatcggc attttgcagc agcgctcata aacctcgata atatcattga
     2341 tacgccctat ttccaattta ggatcgattc cctgcgaaaa catattatag gccacattca
     2401 gcatatcaat atttcccgta cgctctccgt ttccgaacag agtgccctcc actctttcgg
     2461 caccggcaag cagcgcaagc tccgtggcag caatcccggt tcc
//
LOCUS       DAOSYE010000299         2492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32753_length_2492_cov_2.631514, whole genome shotgun sequence.
ACCESSION   DAOSYE010000299 DAOSYE010000000
VERSION     DAOSYE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32753_length_2492_cov_2.631514"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLU43_06005"
     CDS             complement(<1..399)
                     /locus_tag="PLU43_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO28000.1"
                     /translation="MATKQKEPIEEQIIKTEFSDVMQKSYIDYAMSVIVSRALPDVRD
                     GLKPVQRRTLYDMYELGIRYDRPFRKSARIVGDTMGKYHPHGDSSIYDALVVMSQDFK
                     KELPLIEGHGNFGSIEGDGAAAMRYTEARLQ"
     gene            complement(417..2336)
                     /locus_tag="PLU43_06010"
     CDS             complement(417..2336)
                     /locus_tag="PLU43_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOO28001.1"
                     /translation="MTKKTTYDASSITVLEGLEAVRKRPGMYIGSVTTKGLNHLVYEI
                     VDNSVDEHLAGFCDKIWVTLEENGSATVSDNGRGIPVGMHKKGISAERLVFTTLHAGG
                     KFDDSVYKTSGGLHGVGSSVVNALSEYLDIEVMREGKIFHDCYNRGIPAIELENGLLP
                     VIGKTKDTGTKVTFLPDASIFEKTRFKEEEIKSRLHETAYLNPELTIYFQDKRPESAE
                     KLEFHEPDGIIGFVKDMNSKKEVLHEPVYFSGTSEGIAVEIVFQYVNEFHENVLGFCN
                     NIYNAEGGTHLTGFKTTFTNVMNTYARELNILKEKDPNFTGADIRNGITAVVSIKHPS
                     PRFEGQTKTKLDNQDAAKAVGKVTGDEIIRYFDRNLETLKTVLSCAEKAAKIRKTEEK
                     AKTNMLTKQKFSFDSNGKLVNCESRDVQKCEIFIVEGDSAGGSAKTARDRNFQAILPI
                     RGKILNVEKASIDKVLANAEIKTMINAFGCGFSEGYGNDFDITKLRYHKIIIMADADV
                     DGAHISTLLLTLFYRFMPELIYEGHVYIAMPPLYKAMPQKGQEEYLYDDKALAKYRKA
                     HKNFTLQRYKGLGEMDAGQLWETTLDPASRRLKLVEIEDARLASNTTELLMGNEVPPR
                     RQFIYKHAHDAELDI"
BASE COUNT          653 a          608 c          436 g          795 t
ORIGIN      
        1 ctggagccgt gcttccgtat aacgcatggc cgcagccccg tcaccttcta tagagccaaa
       61 attgccatgt ccttctatca aaggcagttc cttcttgaaa tcctgtgaca tgacaacaag
      121 ggcatcataa atagaactgt ctccatgcgg atgatatttt cccattgtgt cgccgacaat
      181 acgggcagac tttctaaacg gcctgtcata ccggatacca agctcataca tatcataaag
      241 agttctcctc tgtaccggct tcaggccgtc gcgtacatcc ggcagcgctc gtgatacgat
      301 aacactcatt gcataatcga tataggattt ctgcatcaca tctgagaatt ccgttttaat
      361 gatctgctct tcaatcggtt ctttttgttt tgtggccata ttgatactgt cctcccctat
      421 atgtcaagtt ccgcatcatg tgcatgctta taaataaact ggcgtcttgg cggaacctca
      481 tttcccatta agagttctgt tgtattggaa gccagtcttg cgtcttcaat ttccacaagc
      541 ttcagccttc ttgaggccgg atcaagtgtt gtttcccaca gctgtccggc atccatttct
      601 ccaaggcctt tataacgctg caatgtaaaa tttttatgcg ctttcctgta tttcgcaagt
      661 gccttatcat catagagata ttcttcctgt cccttttgcg gcattgcttt atacagagga
      721 ggcatggcaa tatacacatg cccttcatag atcagttcag gcataaagcg ataaaacaac
      781 gttaaaagaa gcgttgagat gtgggcaccg tcaacatccg catccgccat aataataatt
      841 ttatgatacc ttaactttgt aatatcaaag tcattgccgt atccttccga aaacccgcat
      901 ccaaatgcat tgatcattgt tttgatctct gcatttgcaa gcactttatc aatacttgct
      961 ttttcaacgt ttaatatttt gccgcggata ggaaggatcg cctgaaaatt ccggtctctt
     1021 gctgttttgg cagaaccgcc ggcagaatct ccctccacaa taaaaatctc acatttctga
     1081 acatccctgc tttcacaatt tacaagcttt ccattcgaat caaacgaaaa tttctgtttt
     1141 gtgagcatgt tggttttcgc tttttcttct gtttttcgga ttttcgctgc tttttccgca
     1201 caggaaagca ctgttttcag cgtttcaaga ttccggtcaa aatagcggat gatttcatct
     1261 ccggttactt taccgactgc ctttgctgcg tcctgattat caagcttcgt tttagtctga
     1321 ccttcaaaac gcggacttgg gtgtttgatc gaaacaacgg ccgtgatgcc gttacggata
     1381 tcggccccgg taaaattcgg atccttttct ttcaaaatat tcaattcccg ggcatatgta
     1441 ttcataacat ttgtaaacgt tgttttaaaa ccggtcagat gggtaccgcc ttccgcatta
     1501 taaatattat tgcagaaacc aagtacattt tcatgaaatt cgtttacata ctgaaaaacg
     1561 atctcaacag caatcccttc tgaagttccg ctgaaatata caggctcatg caaaacttcc
     1621 tttttgctgt tcatatcttt gacaaaccca ataataccgt ccggttcatg aaattccagt
     1681 ttttcagccg attccggacg cttatcctga aaataaatag tcagctcagg attcagataa
     1741 gcagtttcat gtaaacggct cttgatttcc tcttccttaa aacgcgtttt ttcaaatatg
     1801 gaagcatccg gcagaaaagt cacttttgta ccggtatctt ttgtttttcc aataacggga
     1861 agcagtccgt tttcaagttc gatcgccggg attcctctat tataacagtc gtgaaagatc
     1921 tttccctctc tcattacttc aatatcaaga tactctgaca aagcattgac aacagaggaa
     1981 ccaacaccgt gcagtccgcc gctcgtttta tagacagaat cgtcaaattt tccgcctgca
     2041 tgcaatgttg taaatacaag acgttccgca ctgatcccct ttttatgcat gccgaccgga
     2101 ataccgcgcc cgttgtcgga aacagttgcc gaaccgtttt cttccaaagt cacccatatc
     2161 ttatcacaaa atcctgccag atgttcatct acggaattat ctactatttc atagaccaga
     2221 tgattcaatc cctttgttgt aacactgcca atatacatac ctggacgctt tctgacagct
     2281 tccagtcctt caaggaccgt aatactgctt gcgtcataag ttgttttttt agtcatccca
     2341 aatccccttg ctcccgccat gtcatttgcg taattttcct tatatagtat aacatatttt
     2401 taaataattt caatcacaaa gcacaactag ttgtacttat aataaatatc tgtacaacta
     2461 gttgtgcctg ctgacgattc ctgtaaatga at
//
LOCUS       DAOSYE010000300         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32817_length_2489_cov_2.808546, whole genome shotgun sequence.
ACCESSION   DAOSYE010000300 DAOSYE010000000
VERSION     DAOSYE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32817_length_2489_cov_2.808546"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLU43_06015"
     CDS             complement(<1..974)
                     /locus_tag="PLU43_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28002.1"
                     /translation="MTQFLEYLKMAIANIKTNKGRSLLTMLGIIIGISSVIMIISIGN
                     GVKAGINDELNSYAGGQLYVYLDSTKQKENLFEFTQDDFDAIQNKVPHVKAVTPEYYM
                     YGTISGAKGKDLDAVAKAGTEGYQYVVNDPIIKGTYFTRENVENGDKCCVINESGAKS
                     LFGTTENIIGMPVELTLYGSTVELTVIGIRKDSTSAMLNALSGESDRVKLEMPYTVLG
                     SAFGFYIDGFDGAYIVGEGAEYSKEIYKATINLLEARYNVRGEGVFQLQDFNDQLSTI
                     DNVLSTITLFVILVAAISLLVGGIGVMNIMLVSVTERTREIGLRMSIGA"
     gene            complement(1001..>2489)
                     /locus_tag="PLU43_06020"
     CDS             complement(1001..>2489)
                     /locus_tag="PLU43_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO28003.1"
                     /translation="KGLSISVTPVTMGSVEETISTSGFVKSELSKTYFAPVDVTVKEC
                     SCKSGDSVNAGDRLVSFDEQSLALAAQKAALTAVSSSADYNHSLTESSDGSLDYQIAS
                     ANVELYKLLIAAQRTYINDITYAIDNKTYDVSQSAQCVRDSLQKKINAKTEEAASIQK
                     DVNSVSQNAITNPYDPNHQYYVDLQNELTDLTTEQSKLSGALSNTTGIVNTADENRQL
                     AEAQQLLSDMESYLAKDESKKETAENAILDANQKEKLKADTEISQISAEQAADDLALA
                     QAGITSDFNGIVTDVSAQDGAAAAKGSSLLTVESTQDVYVDVTVTKYNLDKISIGQKA
                     DITMAGNSYTGSITKINRKAQNNSQDTPVITAEVHIDDPDDNIFLGVEAKVIIHIAEA
                     SQVLLAPVETVNADNDGSFCYVVKDGIVERRNVESGVTSDTVIEILSGLEEGDLLIDD
                     YTLTIEEGMKVDPIMPDNDDADTVEEESSDADADSSSVTAGAALD"
BASE COUNT          590 a          570 c          561 g          768 t
ORIGIN      
        1 gccccgatag acatccgtaa tccgatttcc cttgtacgtt ccgtaacaga cacaagcatg
       61 atattcatga caccgatgcc tccgacaagc agggagattg cagcaacaag aataacgaac
      121 agagtgatcg tgcttaagac attgtcgatc gtggagagct ggtcgttaaa gtcctgcagc
      181 tgaaagaccc cttctccgcg gacattataa cgggcttcca gcagattgat cgtggcttta
      241 taaatttcct tggaatattc ggctccttct ccaacgatat atgcgccgtc gaaaccgtct
      301 atgtaaaatc caaaggcaga tcctaacacg gtataaggca tttcaagttt tacccggtca
      361 ctttctccgc tcagcgcatt cagcatggca gatgtgctgt cttttcggat gccgatcacg
      421 gtcagttcta ccgtagagcc atatagtgtc agttctaccg gcatgccgat aatattttca
      481 gtagtgccga acagactttt tgcgccgctc tcattgataa cacagcattt atcaccgttt
      541 tcaacatttt cccgtgtgaa ataggtccct ttaatgatcg gatcattcac gacgtattga
      601 taaccttccg tgccggcttt tgcgacggca tcaagatcct ttcccttagc gcccgagatc
      661 gttccataca tataatattc aggtgtgaca gctttcacat gcggtacttt gttctggatt
      721 gcgtcaaaat catcctgtgt aaattcgaac agattctctt tttgttttgt agagtccaga
      781 tatacataga gctgtccgcc tgcatagctg ttcagttcat cattgattcc tgcctttaca
      841 ccattgccga ttgagatgat cataataacg gaggaaatac caatgataat accgagcatt
      901 gtcagcaggg aacgtccttt atttgttttg atattggcaa ttgccatttt cagatattca
      961 agaaattgtg tcatattgct cctcaatatt atttgaaatt ttaatctaaa gccgcgccgg
     1021 ctgtaacgga agaagagtcc gcatccgcat cagagctttc ttcttcaacg gtatcggcat
     1081 catcgttatc cggcatgatc ggatcgactt tcatgccttc ttcgatcgtc agtgtataat
     1141 catcgatcag cagatcacct tcttcaagtc cggataagat ctctattacc gtgtcggatg
     1201 tgacccctga ttctacattg cggcgctcta cgataccgtc ttttacgaca tagcagaagg
     1261 aaccgtcatt atctgcattg actgtttcca caggcgcgag caggacctgg ctggcttctg
     1321 cgatatgaat gatgaccttt gcttcaacgc caagaaaaat attatcgtca ggatcatcaa
     1381 tgtgtacttc ggcggtaatt accggagtat cctgggaatt gttctgtgct tttctgttga
     1441 tttttgtaat gctgcccgta taggagttgc cggccattgt aatatcagct ttttgtccga
     1501 tcgagatctt atcaagatta tatttggtaa cagtaacatc tacgtaaaca tcctgtgtgc
     1561 tttccacagt gagaagagaa ctcccttttg cagcagcagc accgtcttgt gcggatacgt
     1621 ctgttacgat accgttaaag tccgaagtaa taccggcctg tgcgagtgca agatcgtcgg
     1681 cagcctgttc ggcagagatc tgcgatattt ctgtgtctgc cttgagcttt tctttttggt
     1741 tggcatccag gatggcgttt tctgcggttt cttttttgga ttcatctttt gcaagatagc
     1801 tttccatatc ggagagcagc tgctgtgctt ccgcaagctg tctgttttca tctgccgtat
     1861 taacaatgcc tgtcgtgttg gacagcgcac cggataattt actctgttcg gtagtcagat
     1921 cggtcagttc gttttgcaga tccacataat attgatgatt tggatcgtag ggattggtga
     1981 ttgcattttg cgaaacactg ttgacatctt tttgaatact ggctgcttct tcagttttcg
     2041 cattgatttt tttctgaagg ctgtcgcgga cgcattgtgc ggactgtgag acatcgtagg
     2101 tcttattatc gatcgcatag gtgatgtcgt tgatataagt ccgctgtgct gcgatcagca
     2161 gcttgtagag ctccacgttt gcggaagcga tctgataatc taagctgcca tcgctgcttt
     2221 ccgtaaggct gtgattgtaa tcggcactgg aagatacggc ggtaagagcg gctttctgtg
     2281 cagccagtgc aagactctgt tcatcaaagg agacaagccg gtcgccggcg ttcacactgt
     2341 cgccgctttt acagctgcat tctttgactg taacatctac aggggcaaaa taggtcttgg
     2401 aaagttcgct ttttacaaaa cctgatgtac tgatcgtttc ttcaacagat cccattgtaa
     2461 caggtgttac ggaaatggaa agacctttg
//
LOCUS       DAOSYE010000301         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32851_length_2487_cov_3.061266, whole genome shotgun sequence.
ACCESSION   DAOSYE010000301 DAOSYE010000000
VERSION     DAOSYE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32851_length_2487_cov_3.061266"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..845
                     /locus_tag="PLU43_06025"
     CDS             42..845
                     /locus_tag="PLU43_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOO28004.1"
                     /translation="MYIQPQPEPVPEPVAEAFIAAPEPEKEPEPVMEPAPAVVIPEPV
                     PEPVVIPEPIPEPVIVPEPVAAVSAPEAIKEEAEPEPEKIPVVPVFAQEPEVRPAPIN
                     TTREDFTMETGIIISGMSVKGDIISEGSLDVMGNVLGNIEVAGKLEVTGLIEGNSQAE
                     EVFVNGAKIVGEVNSNGSVKIGQNSVVRGNIMAKSAVLAGAVKGDIDVQGPVILDSTA
                     IIMGNIKSKSIQINNGAVIEGLCSQCYADVSPVSFFDEDDKTVKTKKAE"
     gene            874..1569
                     /gene="pyrH"
                     /locus_tag="PLU43_06030"
     CDS             874..1569
                     /gene="pyrH"
                     /locus_tag="PLU43_06030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO28005.1"
                     /translation="MKRILLKLSGEALAGNKKTGFDEDTVRGVALQVKQLVDKGIETG
                     IVIGGGNFWRGRTSENIDRTKADQIGMLATVMNCIYVSEIFRSVGMMTNILTPFACGS
                     FTKLFSKDRANKYFAKGMVVFFAGGTGHPYFSTDTGVVLRAVEMEADGILLAKAIDGV
                     YDSDPGKNPEAKKYREVSIEEVIEKKLQVVDLTASILAMENKMPMYVFSLNSENSIVN
                     TIDGKFNGTKVIV"
     gene            1586..2143
                     /gene="frr"
                     /locus_tag="PLU43_06035"
     CDS             1586..2143
                     /gene="frr"
                     /locus_tag="PLU43_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOO28006.1"
                     /translation="MNEKLMELDEKMKKTIDHLAAEYSAIRAGRANPHVLDKIKVDYY
                     GTPTPIQQVGNISIPDPRMIQIQPWEKNLLKEIEKAIQMSDLGINPTNDGQVIRLVFP
                     ELTEERRKELVKDIKKKGEADKVAIRNIRRDGNDTFKKLLKSEADISEDEVKQLEDEL
                     QKLTNKYIKQVDDMVDEKSKDILTV"
     gene            2282..>2487
                     /locus_tag="PLU43_06040"
     CDS             2282..>2487
                     /locus_tag="PLU43_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOO28007.1"
                     /translation="MNIPNHVAIILDGNGRWAKSKGMPRNYGHVQGSKNVEVICEEAY
                     KLGIQYLTVYAFSTENWSRPEDEV"
BASE COUNT          767 a          422 c          692 g          606 t
ORIGIN      
        1 aacagcctga tgctgcggtc gcagccccgg aactatcatc catgtatata cagcctcagc
       61 cggagccagt accggagccg gttgcagaag cgtttattgc tgcgccggag cctgaaaaag
      121 aaccggaacc agttatggaa cctgcaccgg cggttgtgat accagagcca gtaccggagc
      181 cggtcgtgat accagagcca ataccggagc cagttattgt accggagcct gttgctgcag
      241 tttctgcgcc ggaggccatt aaagaagaag cagagccgga gccggaaaaa attccggtag
      301 ttcctgtttt cgcacaggaa cctgaagtgc gtccggcacc gattaataca acaagggagg
      361 attttacaat ggagacgggt attattattt ctggaatgtc tgtaaaagga gacattatat
      421 cggagggttc cctggacgtt atgggaaatg tgcttgggaa tatcgaagtt gcaggaaagc
      481 ttgaggtcac gggtctgata gaaggcaatt cccaggctga agaagtattt gttaacgggg
      541 ctaaaatcgt tggggaagtt aacagtaatg gttctgtaaa aatcggtcag aattctgttg
      601 tcaggggaaa tattatggca aaatcagcag tgcttgccgg tgctgtaaag ggtgatattg
      661 acgtacaggg cccggttatt cttgattcaa ccgcaatcat catgggcaac atcaaatcca
      721 aatcgattca gatcaacaac ggtgcagtga tcgaaggcct ttgttcacaa tgttatgcag
      781 atgtcagtcc ggttagtttt tttgacgaag atgataaaac ggtaaagacg aagaaagcgg
      841 agtagagtac tctgaataag gatggcagtt atgatgaaga gaatactgtt gaaattaagc
      901 ggagaggcgc ttgcaggaaa taaaaagacg ggatttgacg aggatacggt aagaggtgtt
      961 gcgcttcagg tgaagcagct ggtcgataaa gggatcgaga cgggaattgt gatcggcggc
     1021 ggcaattttt ggagaggacg taccagtgaa aatattgacc ggaccaaggc agatcagatc
     1081 ggaatgctgg ctacggtcat gaattgtatt tatgtgtctg agatatttcg ttcagtcggg
     1141 atgatgacaa atatactgac gccgtttgca tgtggttcat ttacaaaatt gttttcaaaa
     1201 gatcgtgcaa ataaatattt tgcgaagggt atggtcgttt tttttgccgg cgggacagga
     1261 catccttatt tttcgacaga taccggtgtt gtactcaggg cagttgaaat ggaggcggat
     1321 gggatcttgc tggcaaaagc gattgatggt gtttatgaca gcgatcccgg gaaaaatccg
     1381 gaggcgaaga aataccggga ggtttcgatc gaagaggtca ttgagaagaa attacaggtc
     1441 gttgatctga cggcatccat tcttgccatg gaaaataaaa tgccgatgta tgtgtttagt
     1501 ttgaacagtg aaaacagtat tgttaacaca attgacggaa agtttaacgg tacaaaggtt
     1561 attgtataaa ttgggagggt atcagatgaa tgaaaaatta atggaacttg acgaaaagat
     1621 gaaaaagacg atcgatcatc ttgcggctga gtattctgca atccgtgcag gacgtgcaaa
     1681 tccgcatgtg cttgataaaa ttaaggtgga ttattatggt acgccgactc cgattcagca
     1741 ggtaggcaat atttcgattc cggatccgcg tatgatacag atccagccgt gggaaaagaa
     1801 tctgcttaag gaaatcgaaa aagcgatcca gatgtccgat cttgggatca acccgacaaa
     1861 tgacggacag gtgatcaggc ttgtttttcc ggaactgacg gaggagagaa gaaaagagct
     1921 tgttaaggat atcaagaaaa aaggcgaagc tgataaagtg gcgatcagaa atatcagaag
     1981 agacggaaat gacactttta aaaagcttct gaaatctgag gctgatattt ccgaagatga
     2041 ggtcaagcag ctggaggatg agcttcagaa gctcacaaat aaatatatca aacaggtgga
     2101 tgatatggtg gatgaaaagt caaaagatat tttgacagtt tagatcctgt agcgcctgtg
     2161 ataagaaaag atttaattga atcatcaggg ggacaagacg aggcaatccg gtcgtgtccc
     2221 ctgtcatatt tatgcagggc tgttttaaag cggtcataat accgatatgg aggacggttg
     2281 aatgaatata ccaaatcatg ttgccattat tctggacgga aacggcagat gggcgaagag
     2341 taaaggcatg ccaagaaatt acggacatgt acagggcagt aaaaatgttg aagttatatg
     2401 tgaagaagca tataagctcg ggatacaata tctgacggtc tatgcgtttt caacagaaaa
     2461 ctggtccagg ccggaggatg aggtaga
//
LOCUS       DAOSYE010000302         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32890_length_2486_cov_2.764295, whole genome shotgun sequence.
ACCESSION   DAOSYE010000302 DAOSYE010000000
VERSION     DAOSYE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32890_length_2486_cov_2.764295"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLU43_06045"
     CDS             <1..203
                     /locus_tag="PLU43_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOO28008.1"
                     /translation="MITKEYVKEGAVVIDVGIHRMDNGKLCGDVDYDNVFKKCSAITP
                     VPGGVGPMTIAMLMNNCVEAMK"
     gene            232..1161
                     /locus_tag="PLU43_06050"
     CDS             232..1161
                     /locus_tag="PLU43_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO28009.1"
                     /translation="MVCPKCNAELKEGHLYCENCGAEIRIVPDFEPEIEESMNLSLSG
                     VAEAISEPQSIENEQKKKSQKKRRAKSGKKMMVTSSLAFFALCVVGVIVIGLSRYYSY
                     DYQYEKAMREYESGNFESAVITAKHAVSINPDEEKSKLLLADTYFSLQKYDESIAVLM
                     GMLDLFPDDSDIYERLVANYEIKGDVESIGELIKNCGDNTIMAEFDAYVSVAPSFSIE
                     EGTYYEAEFLSLLSDGNGTIYYTLDGELPDEHSKKYKSPISLKEGTTVVSAIYVNEKG
                     IVSDPVQKTYTIKFKLPKVPVLLTKAGTTIPRY"
     gene            1167..1619
                     /locus_tag="PLU43_06055"
     CDS             1167..1619
                     /locus_tag="PLU43_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO28010.1"
                     /translation="MKEPKEGIIYYTDDGSDPTKESEIYTLPIPMPLGTSQFKFVNIS
                     KDGIASEIITAEYTLQIATIVDTTAAEAAIQFFLQTKGDTTAGCEYLCTSAYGAGSRT
                     YYMIDEYENSSGNLQKTGRIFAVDVCTGELYKANINIVEGDYEFSFLY"
     gene            1712..>2486
                     /locus_tag="PLU43_06060"
     CDS             1712..>2486
                     /locus_tag="PLU43_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOO28011.1"
                     /translation="MYKILKAEMLAENIYLMDVEAKRVAKACMPGQFVIVKVDKKGER
                     IPLTICDYDREAGSITIVFQTVGGSTERMSNLKAGDSFRDFTGPLGQPSEFVHEDPET
                     LKGKKFLFVAGGVGTAPVYPQVKWMHEQGIAVDVIVGAKNKELVILEKEMEAVAGNLY
                     VTTDDGSYKRKGMVTEVIKDLVQNQGIKYDHCVAIGPMIMMKFVCILTKELGLPTTVS
                     LNPIMVDGTGMCGACRVTVGGKVKFACVDGPEFDGHAVNF"
BASE COUNT          759 a          421 c          628 g          678 t
ORIGIN      
        1 aaatgatcac aaaggaatat gtgaaagaag gagccgtagt gatcgatgtc ggcattcacc
       61 gaatggataa cggaaaactc tgtggtgatg tggattatga caatgttttt aaaaaatgca
      121 gtgcaatcac gccggttccg ggcggagtcg gtccgatgac gattgcgatg ctgatgaata
      181 attgtgtaga agccatgaaa tagtcatatc tggctgaaac gggtgaagca tatggtatgt
      241 ccgaaatgta atgcggagtt gaaagaaggt catttgtatt gtgagaactg cggtgcggaa
      301 attcggatcg ttccggattt tgaacctgaa atcgaagaga gcatgaatct ttctctttca
      361 ggcgtagccg aggctatttc agaaccgcag tcaatagaaa atgaacaaaa gaagaaatcc
      421 caaaaaaagc gaagggcgaa atcagggaaa aaaatgatgg tcacttcttc gcttgctttt
      481 ttcgcgctgt gtgtcgtcgg tgtgatcgtg atcggtctca gccgttatta ttcttatgat
      541 tatcagtatg agaaagccat gcgggagtat gaaagcggaa attttgaatc agccgttata
      601 actgcaaagc atgcggtttc cattaatccg gatgaagaaa agtcaaaact gcttttggca
      661 gatacttatt tttcactcca gaaatatgat gaatcgatcg cggtactcat gggtatgctt
      721 gatctgtttc cggatgattc agacatttat gaacgtcttg ttgcaaacta tgaaataaag
      781 ggcgatgtgg aatcgatcgg tgaactgatc aaaaactgcg gcgacaatac gatcatggct
      841 gaattcgatg catatgtatc agttgctcct tcattcagta tagaggaagg cacttattat
      901 gaggcggagt tcttaagcct gctgtcggat ggcaatggca cgatttatta tacactggat
      961 ggtgaactgc cggatgagca ttcgaaaaaa tataaatcgc cgatttcact gaaagaagga
     1021 acaaccgttg tatctgccat ttatgtcaat gaaaaaggaa tcgtcagcga ccctgtgcag
     1081 aaaacttata cgatcaaatt taaattgccg aaagttcctg tgctgttaac aaaagcggga
     1141 actacgattc cccggtactg ataaagatca aagaaccgaa agaaggcatt atctattata
     1201 cggatgatgg aagtgatccg actaaggagt ctgagatcta tacacttcca atcccaatgc
     1261 cgcttggtac cagccagttt aagtttgtca acatcagcaa ggacggcatt gcaagtgaga
     1321 tcattactgc ggagtataca ctgcagatcg ccacgatcgt agacactacg gcagcggaag
     1381 ccgcaattca gtttttcctg cagacaaaag gcgatacaac agccggctgt gagtatttat
     1441 gtacatctgc ctatggagcc ggaagccgta cctattatat gattgacgaa tatgaaaatt
     1501 ccagcggtaa tctgcagaaa accggtcgta tttttgcggt cgatgtatgt acaggggaat
     1561 tatataaggc aaacatcaat attgtggaag gtgattacga attttctttt ttgtattaaa
     1621 aagattgtct tttttctgtc aatgaattat attattaagt ggattatttt ttttaaataa
     1681 taatagataa taattaggag gagcaaggtg tatgtacaaa attttaaaag ctgaaatgct
     1741 tgctgaaaac atttatctga tggatgtaga agcaaagcgg gttgcgaaag cctgtatgcc
     1801 cggacaattt gttattgtaa aggtggataa aaagggagag cgtattccgc ttacgatctg
     1861 tgactacgac agagaagcag gttcgatcac aattgtattt cagacagtgg gtggttctac
     1921 agaaagaatg tctaacttaa aagctggtga cagcttcagg gattttacag gtccgcttgg
     1981 tcagccgtcg gaatttgttc atgaggatcc ggaaacacta aagggcaaaa agtttttatt
     2041 tgttgcgggc ggagttggta ccgcgcctgt atatcctcag gtgaagtgga tgcatgaaca
     2101 aggaatcgct gtagatgtta tcgttggcgc aaaaaataaa gagttggtca ttcttgaaaa
     2161 agagatggaa gcggttgccg gtaatctgta tgtgacaact gatgacggtt cctataaaag
     2221 aaagggcatg gtcacggaag tgatcaaaga ccttgttcag aatcagggaa tcaaatatga
     2281 ccactgtgta gcgatcggcc cgatgatcat gatgaaattt gtatgcattt tgacaaaaga
     2341 gctggggctt ccaacaactg taagtctgaa tccgatcatg gtagatggta ccggtatgtg
     2401 tggtgcctgc agggttactg ttggcggaaa agtgaaattt gcctgtgtgg acggaccgga
     2461 atttgatggc catgcagtga attttg
//
LOCUS       DAOSYE010000303         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32892_length_2486_cov_2.453723, whole genome shotgun sequence.
ACCESSION   DAOSYE010000303 DAOSYE010000000
VERSION     DAOSYE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32892_length_2486_cov_2.453723"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLU43_06065"
     CDS             complement(<1..207)
                     /locus_tag="PLU43_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426903.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOO28012.1"
                     /translation="MSKKPTVLMILDGYGLNDKKEGNAIYEAKTPVIDGLMRDYPFVK
                     GLASGMAVGLPDGQMGNSEVGHLNM"
     gene            complement(338..1150)
                     /locus_tag="PLU43_06070"
     CDS             complement(338..1150)
                     /locus_tag="PLU43_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOO28013.1"
                     /translation="MMVYFIQFMIFLLFLCAAWLIKLFNQGTLAYRQYRKEKQKDKAY
                     IAAEKTIEMLREEFRGKPTKFENYTAEVFKSAGYEDVTVTQASNDGGKDLIMYKNEKK
                     YVAEVKLYHPACKISRERIQKLHSAMLDCEADRAVFVTTSDFTDPAVVYAGKHGIELV
                     NGYLLGRWIRLLEEADKDGKNQLKFLHETMEEDREFVRQGNKAKAWRIMGLFLLLYPL
                     YSVCWIFLLNRQLIYVNAFYGWILAELVLFNAVDLRIFTKCLIRGRQKQSSL"
     gene            complement(1257..1595)
                     /locus_tag="PLU43_06075"
     CDS             complement(1257..1595)
                     /locus_tag="PLU43_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO28014.1"
                     /translation="MEEKRKNRRMELEAHLIMKRIDSGKNEKIPVDVLNLSKTGIGFK
                     CEKLLEINSVYEAELIIWTREIIHTFIDVTRFDNSGDDNIYGALFIGMPESDACKIEI
                     YDMFDKASHK"
     gene            complement(1695..>2486)
                     /gene="queA"
                     /locus_tag="PLU43_06080"
     CDS             complement(1695..>2486)
                     /gene="queA"
                     /locus_tag="PLU43_06080"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOO28015.1"
                     /translation="KRKENDIWETLVRPGRKLRPGAKLSFGGGLLKAEIMEIVEEGNR
                     LVKFFYDGIFEEVLDQLGEMPLPPYIRHELKDRNRYQTVYAKYEGSAAAPTAGLHFTK
                     ELLQSLEDGGITIAYVTLHVGLGTFRPVKAENISEHHMHSEYYQITREAAEKINRAKT
                     GGHRVICVGTTSCRTIESAADENGHLEESCGNTEIFIYPGYRFKVLNCLITNFHLPES
                     TLLMLVSALAGREHVLSAYRKAVEERYRFFSFGDVMLITDNNSIS"
BASE COUNT          639 a          647 c          382 g          818 t
ORIGIN      
        1 catattcaga tgtccgacct cggaattgcc catctgtccg tccggaagtc caacagccat
       61 gccgcttgca agtcctttta caaatggata atcccgcatc agtccgtcta tgaccggagt
      121 ttttgcttca tatatggcat ttccttcctt tttatcattt aatccatatc cgtctaaaat
      181 catcaatacc gtaggttttt tactcatcct tttctccttc taccatatct gtttcattat
      241 ttatctgcta tctttttttg ctcatgttag gaattataca ctttttggga cacgtccgca
      301 atatcttctt gcaaaggggt ttctttttat tagaaaatca taaagaactc tgcttctgcc
      361 tgcctcttat cagacatttt gtaaaaattc taagatcaac ggcattgaat aacactaatt
      421 ctgccagtat ccagccataa aaagcgttca catagataag ctgcctgttc agtaaaaata
      481 tccagcagac cgaatataat ggatacagaa gcaaaaacag tcccatgatt ctccaggctt
      541 ttgctttatt tccctgcctg acaaactctc tgtcctcttc cattgtctca tgcagaaact
      601 tcagctgatt ttttccgtct ttgtctgctt cctcaagcag tctgatccat ctgcccaata
      661 aatacccgtt tacaagctcg attccatgtt ttcctgcgta gacgaccgca ggatccgtaa
      721 aatcacttgt cgttacaaaa acagctctgt ccgcctcaca gtccagcatt gcagaatgca
      781 gtttctgaat acgctccctg ctgattttac atgccggatg ataaagcttt acctccgcaa
      841 catatttttt ttcattttta tacatgatca gatctttccc gccgtcatta cttgcctgcg
      901 ttaccgttac atcctcataa cccgcacttt taaatacttc tgccgtataa ttctcaaatt
      961 tcgtcggttt tcccctgaat tcctcacgca gcatttcgat cgtcttttcc gccgcaatat
     1021 aggccttatc cttttgtttt tccttacggt attgtctgta ggcaagcgtc ccctgattaa
     1081 acaattttat cagccaggcc gcacataaaa acaagagaaa tatcataaac tgaataaaat
     1141 aaaccatcat cttcacttct gcttctttct gctttattgg ataataaata agaagccctt
     1201 tctgtaaacg gacttcttat ttattttata aaattatcat ttggcggcaa tgtcagtcat
     1261 ttatgacttg ctttatcaaa catatcatag atctctatct tgcacgcatc agactccggc
     1321 atgccgataa acaacgcacc gtaaatattg tcatcaccgc tgttatcaaa tcttgttaca
     1381 tcaataaacg tatgaatgat ctctcttgtc cagataatca gttccgcctc atatacggaa
     1441 ttgatttcaa gaagcttttc gcacttaaat ccgatacccg ttttagataa attcagaaca
     1501 tccaccggta tcttttcatt ctttcccgaa tcgatccttt tcatgatcaa atgcgcttcc
     1561 agctccattc gtctgttttt tctcttttct tccatgggat acccctttcc atccctctac
     1621 ccgcagcgcc ttcctgcatc tgccgtattt ataaaataat catataatac ttttttgttt
     1681 cttacaagct aaagttacga aatactgtta ttatctgtaa taagcattac atctccaaat
     1741 gaaaaaaatc tgtacctttc ctcaactgcc ttccgataag ccgacagcac atgctccctg
     1801 cctgcaagtg ccgatacaag catcagaagc gtggattccg gtaaatgaaa attcgtaatc
     1861 aggcagttaa gaactttaaa tctgtatccc ggataaatga aaatttccgt attgccgcag
     1921 ctttcctcaa gatgtccgtt ttcatctgct gcactttcta tcgtcctgca gctcgttgta
     1981 ccaacacaga tcacgcgatg accgcccgtt tttgcccgat tgatcttctc tgctgcttcc
     2041 ctcgtaatct gatagtattc cgaatgcata tgatgctccg atatattttc agccttaacc
     2101 ggccgaaatg ttccaagtcc gacatgcagt gttacatatg caatcgtaat tccgccgtcc
     2161 tcaagagatt gcagcagttc tttcgtaaaa tgcagtccgg cagtcggcgc agcagccgaa
     2221 ccttcatatt ttgcatacac tgtctgatac cgattcctgt ccttaagttc atgtctgata
     2281 tatggaggca gcggcatttc tcccagctga tccagcactt cctcaaagat cccgtcataa
     2341 aaaaatttca caagccggtt gccttcctca acgatttcca tgatctctgc tttcagcaga
     2401 ccgccgccaa agctgagttt tgcccctggc cgaagctttc ttccaggtct gacaagtgtc
     2461 tcccatatat cattctcttt tctttt
//
LOCUS       DAOSYE010000304         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_32907_length_2485_cov_3.244856, whole genome shotgun sequence.
ACCESSION   DAOSYE010000304 DAOSYE010000000
VERSION     DAOSYE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32907_length_2485_cov_3.244856"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PLU43_06085"
     CDS             complement(<1..1337)
                     /locus_tag="PLU43_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO28016.1"
                     /translation="MKKKQLLSAVLASVMVFSLTACGSSSTESQTDTGTAETETETAA
                     ETETTTETAAADTEAVSEEDKVITFWNLGTDEPDKSLWQAAADWYNENESPTSGYTVE
                     TVSTTNDQYKEKLSVAMSAGECPDMYIHWTGGPMVEYIKSGYAQDITDLMTDSGTKDK
                     YLDAAINQSTYDGKIYAVPVLNDSVATFFYDKKMWADNGYEVPTTIDELEALCDQMVA
                     DGVTPFALANSSQWTGSMYYMYLVARYGGPDIITNAYDGSGSFVNEATQYAGEKIEEW
                     VENGYFPEGVNSLSADDGEDRALLYQGAGMMLQGSWQVGSIKQENPDFYENLASFAFP
                     VLEGSSADQSVVVGTIGDNFVSFNCTGEKLQEAFKVAEYYSSSEWEAQAIEAGKVPPL
                     KDATSEEPTWQDILSIISDASSVQLWWDQYLPSSVADVHKSASQKLFDLSATP"
     gene            complement(1459..>2485)
                     /locus_tag="PLU43_06090"
     CDS             complement(1459..>2485)
                     /locus_tag="PLU43_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28017.1"
                     /translation="IGSHIVRNNVIHDCGQNGIVGHLGCIFSEIYGNEIYSIATKHEF
                     YGYEIGGIKLHAAIDVQIYDNNIHHCTLGTWLDWEAQDTRVSRNLYYQNDRDLMVEVT
                     HGPYIVDNNIFASDYNFDNVAQGGAYINNLCCGTMRRQDVLDRSTPYHFPHTTQMMGT
                     SFVYGGDDRLYQNIFLGGAPIYNKEQSKYGTEDYDGCPASYEEYISLIKEALPGDENL
                     YVLVKQPAYIDRNVYLNGAKAYAREEHKFVSEKDPQVKIVREGDETFLNITVEKGVLE
                     QCAQVVHSESLGYNRAAEAPFDNPDGTPIAFDTDYNGVKRTDQVSAGPLENLTEGENK
                     IKIWCKK"
BASE COUNT          625 a          642 c          486 g          732 t
ORIGIN      
        1 tcaggcgttg cggaaagatc aaacaatttc tgcgaagcag atttatgaac atcggcaacg
       61 gacgatggaa gatactgatc ccaccaaagc tgtacggaag aagcgtcgct gataatactc
      121 aaaatatcct gccatgtagg ctcctctgaa gtagcatcct taagcggcgg cacctttcct
      181 gcttcaattg cctgtgcttc ccattcggat gaagaataat attctgctac cttaaacgct
      241 tcctgcagct tctctcccgt gcagttaaag gaaacgaagt tatctccgat cgttcctact
      301 acaacactct ggtctgcgga tgagccttca agaaccggga atgcaaagga cgcaaggttt
      361 tcataaaagt ccggattttc ctgcttgata gaaccaacct gccaggaacc ctgcagcatc
      421 atgcctgctc cttggtataa aagtgcacga tcttcaccgt catctgctga aagcgaattc
      481 acgccttccg gaaaatatcc gttttctacc cactcttcaa ttttctcccc tgcatattgc
      541 gtagcctcgt ttacaaaact tccggaaccg tcataggcat ttgtaataat atccggtccg
      601 ccgtagcggg caaccagata catataatac atggaaccgg tccactggga actgttagcc
      661 agtgcaaacg gcgtaacacc gtcagctacc atctggtcac aaagtgcttc cagttcatcg
      721 atcgttgtcg gaacttcata tccattgtcg gcccacattt tcttatcata gaagaaagtc
      781 gccacggagt cattcagaac aggaaccgca tagatctttc cgtcataggt agactgattg
      841 atcgctgcat ccagatattt gtcttttgtt ccgctgtctg tcatcaggtc tgtaatatcc
      901 tgcgcatatc cggacttgat atattcaacc atcggaccgc ctgtccagtg aatatacata
      961 tccgggcatt cgccggcact cattgctacg gaaagctttt ctttgtactg atcgtttgtc
     1021 gttgaaacgg tctctaccgt atagcctgac gtcggagatt cgttttcatt gtaccagtct
     1081 gctgctgcct gccaaagtga tttatccggc tcgtctgtcc caagattcca gaatgtaatg
     1141 accttgtctt cttcagatac tgcttcagta tctgcagcag ccgtttccgt cgttgtttcc
     1201 gtttctgctg ctgtttctgt ctctgtttcg gccgttccgg tatctgtttg tgattccgtt
     1261 gatgatgagc cgcaggctgt cagtgaaaac accatcacag aagcaagaac tgctgataat
     1321 aactgtttct ttttcatgtt tttcctcctt ttgataactc aataaagcat attttatact
     1381 tcaccttgta acaacttaag gataacattt ttttactgta tgaaaaataa aatactttga
     1441 cagaaatcta taattttttt actttttaca ccaaatcttt attttgttct ctccttccgt
     1501 cagattctca agcggaccgg ccgaaacctg atccgtacgc ttcactccgt tataatccgt
     1561 atcaaaagca atcggtgttc cgtccggatt gtcaaacggc gcttccgcag cacggttata
     1621 tccgagactt tcggaatgaa ccacttgtgc acactgctcc agtacgcctt tttcgactgt
     1681 tatattcaaa aacgtctcat cgccttccct gacgatcttc acctgcggat ctttttcgct
     1741 gacaaattta tgctcttcac gggcatacgc ttttgctccg ttcagataaa cattgcggtc
     1801 aatataagcc ggctgtttaa caagaacata caaattttcg tcacccggca gtgcctcctt
     1861 gatcagactg atatattctt cataggaagc cggacagccg tcataatcct ctgttccgta
     1921 ttttgactgt tccttattat agatcggtgc tccgccaagg aaaatattct gatacagacg
     1981 gtcgtcccct ccatatacga aagacgttcc catcatctgc gtcgtatgtg gaaaatgata
     2041 cggcgtggag cggtccagga catcctgcct gcgcatcgtg ccgcagcaga gattattaat
     2101 ataagcaccg ccctgcgcaa cattgtcaaa attataatcc gatgcaaaaa tattattatc
     2161 cacgatatac ggaccgtgtg ttacttcgac catcagatca cgatcattct gatagtacag
     2221 atttctgctg acccttgtat cctgtgcctc ccagtcgagc catgtaccaa gcgtacagtg
     2281 gtgaatatta ttgtcataga tctgcacatc gatcgcagca tgcagcttga ttccgccaat
     2341 ttcatatccg taaaactcat gctttgtcgc aatactgtag atctcatttc catagatctc
     2401 gctgaaaata catcccagat gaccgacaat cccgttctgc ccacagtcat ggatcacatt
     2461 gttgcggacg atatgggaac cgatc
//
LOCUS       DAOSYE010000305         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33056_length_2478_cov_3.117623, whole genome shotgun sequence.
ACCESSION   DAOSYE010000305 DAOSYE010000000
VERSION     DAOSYE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33056_length_2478_cov_3.117623"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLU43_06095"
     CDS             complement(<1..241)
                     /locus_tag="PLU43_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOO28018.1"
                     /translation="MNAVNYQKELDQLLSGLQDGKRPKLLLHSCCAPCSSYCLTYLSP
                     YFDITVFYYNPNIFPKEEFLKRAEEEKRLITQLNEE"
     gene            complement(210..>2478)
                     /locus_tag="PLU43_06100"
     CDS             complement(210..>2478)
                     /locus_tag="PLU43_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO28019.1"
                     /translation="HKMIKLLVTCAGLLLACIFICLLHLQKVVSMYRQLTYDAIYTQT
                     DMGAEIIGKTFQRDCNIIYSEAQEISRKGVRSSEEIVDMLSSMSRKVSFEKIYFINAA
                     GMAYYPEGEVFQADEDALTEHVDMSKDVSIYLDKKYFSDGIYTFSVVSPVVEDGITQG
                     YLVGVDQTDTLLDGVKTAYYTTQISECYVMDNDGLIIAAMQTKTDQMSIGSNFYTELQ
                     KQISDETEAEQFESSLRQGLASGELGNINVELNKDDMHLFYTSISEADGWNLVYCISQ
                     NAIRKQVWPITLEVLCTCAGILFVMILLGEVVWKYVGEDQKQIEKLAYKDMLTEAPNE
                     TSFKIRVKELLEEYHELPYVIICYDILNFRYINEGYGHAKADMVLKAMVEASWESFSY
                     NETFARLSADMFICLSVNDGRYKERRKFLEERLSKAAGSIYMHYPVKIKCGFYAVTDY
                     REDISDMIDKANLARKAVDINSRDLEAEYVESLMEDTRKREYIESKMESALENGEFVP
                     YLQPKWDMKLNRISGAEALVRWRKPDGEIIPPNEFIPIFEKNGFIEKIDFYMLEKVCK
                     YLRKMLDEGKVVYPVSINQSRYLLHDPEYTLHIQEILLKYKIPKGLVELELTETVFFQ
                     ERERMLEVMKQLKELNMDLSIDDFGSGYSSLNLLRDIPFDVLKIDRGFLDKTKTSDTG
                     KWILRKIVEMAEGLGVKVICEGVETQEQVAMLLEIGCIYVQGFLYSRPIPLEEFITKY
                     NVEQKDECRKLSEGT"
BASE COUNT          676 a          649 c          409 g          744 t
ORIGIN      
        1 attcttcatt taattgcgta atcagacgct tctcttcttc tgcccgcttt aagaattcct
       61 cttttggaaa aatattggga ttataataaa ataccgtgat atcaaaataa ggggaaagat
      121 aagtaagaca atagctgctg catggtgcac agcagctatg cagcagcaat ttgggtcttt
      181 ttccgtcctg aagtccggac agcagctgat caagttcctt ctgataattt acggcattca
      241 tctttttgct ccacattata tttagtgata aattcttcga gcggaatcgg ccgggaatac
      301 aaaaatccct gtacgtaaat acagccgatc tcaagcagca tcgctacctg ctcctgcgtc
      361 tccacacctt cacagatcac cttaacacct aatccctccg ccatttccac gatcttgcgg
      421 aggatccatt tgcccgtatc actcgtcttt gttttatcca aaaatcctct gtcgatcttc
      481 aaaacatcaa acggaatgtc acgcagcaga ttcagggaag aatatccgga tccaaaatca
      541 tcgatcgaca gatccatatt cagctctttc agctgcttca ttacttcaag catccgttca
      601 cgctcctgaa agaacaccgt ttccgtcagt tccagctcca caagaccctt cgggatttta
      661 tatttcaata aaatttcctg gatatgcagt gtatactccg gatcatgcag cagatatctg
      721 gactggttga tggaaaccgg atataccact tttccttcgt ccagcatttt ccttaaatat
      781 ttacacactt tttccagcat ataaaaatcg atcttttcaa taaatccatt tttttcaaat
      841 atcggaataa actcattcgg cggaatgatc tcaccgtcag gcttacgcca gcgcaccagt
      901 gcctcagctc ccgagatcct gttcagcttc atatcccatt ttggctgcag atacggtaca
      961 aactcaccgt tttccaatgc actttccatt ttggattcga tatattcccg ctttctcgta
     1021 tcttccatca ggctttccac atattcggct tccagatccc tggaattgat atcaacagct
     1081 tttcttgcca gattcgcctt atctatcata tcagagatat cttcccgata atcggttacc
     1141 gcataaaatc cgcattttat ttttacggga taatgcatat aaatggaacc ggccgccttg
     1201 cttaaccgtt cctctaaaaa ttttctccgt tctttatacc ggccgtcatt cacagacagg
     1261 cagataaaca tatcggcact cagtcttgca aaggtctcat tataactgaa gctttcccag
     1321 gaagcctcta ccattgcctt cagcaccata tccgccttgg catgtccgta tccttcattg
     1381 atataccgaa aattcaatat atcatagcat ataataacat acggcaattc atgatactct
     1441 tccaaaagct cctttaccct gatcttaaaa ctggtttcat taggtgcctc tgtcagcata
     1501 tctttataag ccagcttctc gatctgcttc tgatcctcac caacatattt ccagacaacc
     1561 tctccaagca ggatcataac aaacagaatc cctgcgcagg tgcacagcac ttccagcgtg
     1621 ataggccata cctgctttcg gatcgcattc tgcgaaatac agtaaacaag attccagccg
     1681 tctgcttccg aaatagaagt ataaaacaaa tgcatatcat ctttgttcag ttcaacattg
     1741 atatttccca attcaccgct ggcaagcccc tgccgtaaag atgactcaaa ctgctctgct
     1801 tctgtttcat cggatatttg tttctgcagt tcggtataga aattacttcc gatactcatc
     1861 tgatctgtct tggtctgcat ggcggcaatg atcaggccat cgttgtccat cacatagcac
     1921 tcggatatct gcgtcgtata ataggcggtc ttcactccat caagcaatgt atccgtctga
     1981 tcgaccccta ccagatatcc ctgtgtgatc ccatcctcaa caacaggaga aacaacagaa
     2041 aatgtataga tcccgtcact aaaatatttt ttgtccaaat aaatactcac atctttcgac
     2101 atatctacat gttcggtgag tgcatcctca tctgcctgaa acacttctcc ttccggataa
     2161 tacgccatac ccgccgcatt gataaaataa attttttcaa aagatacttt tctgctcata
     2221 cttgaaagca tgtcaacgat ctcttccgaa gagcggaccc cttttctcga gatctcctgc
     2281 gcttctgaat aaataatatt acagtccctt tgaaatgtct ttcctatgat ctccgcgccc
     2341 atatctgtct gcgtatagat cgcgtcatag gtgagctgtc tgtacatgct gactaccttt
     2401 tgtaaatgca gcaggcatat aaaaatgcag gccaaaagca gtccggcaca tgttacaagc
     2461 agcttgatca tcttatgc
//
LOCUS       DAOSYE010000306         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33091_length_2476_cov_4.009500, whole genome shotgun sequence.
ACCESSION   DAOSYE010000306 DAOSYE010000000
VERSION     DAOSYE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33091_length_2476_cov_4.009500"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLU43_06105"
     CDS             complement(<1..342)
                     /locus_tag="PLU43_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOO28020.1"
                     /translation="MIRPTLEEVKKIAEQGDYKIVPVSMEILSDVRTPIEVLRILKKV
                     SDHCYMLESAASNEKWGRYTFLGYDPKQEIVCTDHQMTVNHMTFQAVNPGIYIRQLLR
                     EHKSPVMDYLPS"
     misc_binding    complement(409..648)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(689..2347)
                     /locus_tag="PLU43_06110"
     CDS             complement(689..2347)
                     /locus_tag="PLU43_06110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO28021.1"
                     /translation="MLKTESAVLFAVFGILTVFIIIMVEKKEISRIVDLTKSVQAVSF
                     TENRFPKISVGGKDEIALLGKNINCMLMELEKNYDAIKKNNEHLYHAMEVTNDGYFDY
                     DITNQTIDISQTWLKYLGYENNLDLVTDIGKVLDGILPEDRQRFEEALHAFIQNGGEL
                     FYEEVRAYKAQHGYIWVLVRGGIIEYDREGNAVRFVGSLSDITKRKENENQKIFLLQT
                     DPVTGLKNRAYMEKKLEEYKQEEDPVFSILMADVNGLKLINDAFGHREGDRLLKTIGD
                     IITYCCDDADIPVRWGGDEFLVLVRNNRTYAENLMRRIKYELDLIVSFPIKISVAAGC
                     SAYKKTDMDMEQVISRAEEKMYRNKLLESRSFHNGIIVTLEQTLYEKKIELPAAIERR
                     QKICRDIGKQMHLSQETLDELDLLNLLHDIGKIELPDDLIFKTGGMTEEEIGRKKKHT
                     ETGYRIVKTIPEIAHIANSVLYHHESFDGSGYPTGISGDEIPITSRIFSVVNYFDFLT
                     NRNGQNSVGQEEAKQMVMEKAGSKFDPEVVSVFIKVTEKKEYMD"
BASE COUNT          638 a          620 c          409 g          809 t
ORIGIN      
        1 cgaaggaagg tagtccatga caggactttt atgttccctt aacagctgcc ggatataaat
       61 acccggattt accgcctgaa atgtcatatg atttacagtc atctgatgat ctgtgcagac
      121 gatctcctgc tttggatcat atcctaaaaa agtatatctt ccccattttt catttgatgc
      181 cgcggattcc agcatataac aatggtcgga aacctttttc agtattctta ggacttcgat
      241 cggggtcctg acatccgaaa gaatttccat actgacagga actattttat aatctccctg
      301 ctctgctatt ttttttactt cttctaaagt tggacgtatc atttttccct ccatcatgta
      361 attattttct tatgaaacaa aaaagtccct gacaaaaata tactttctgt caaggacgaa
      421 ttattctttc gcggtgccac ctttatttgc agcaatgctg cacactctgc aggatacaaa
      481 catacccccg gcaattaacg gatgccttcc cgttgcggaa tactcaacca aaccgatctt
      541 tgaccgcacc ctccatggtc catttgatga cttgtttttc gccctttctc agcatccgga
      601 ctctctgtag aagcatgatc accgttatct ccacttcatt ggtttcatca ttatgttata
      661 aagagtataa agcagacgaa tgcctttgtc aatccatata ttcctttttt tccgttactt
      721 ttataaatac acttacgacc tcaggatcaa attttgaccc ggccttttcc ataaccatct
      781 gctttgcttc ttcctggccg acagaattct gtccgtttct gtttgtcaga aaatcaaaat
      841 aatttactac cgaaaaaatc ctggacgtga tcggtatttc atcaccggaa ataccggtcg
      901 gatacccgga gccgtcaaag ctttcatgat gatacaaaac actgttggca atatgtgcga
      961 tctccggaat cgtttttaca atacgatagc ctgtttccgt atgtttcttt ttacggccga
     1021 tctcttcctc cgtcatcccg ccggttttaa atataagatc atccggcagt tcgatttttc
     1081 ctatgtcatg cagtaaattc agcaagtcca gttcatccag ggtttcctgg gaaagatgca
     1141 tctgctttcc gatatccctg cagattttct gccttcgttc aattgcggca ggcagttcaa
     1201 ttttcttttc atataaggtc tgctctaatg tgacaatgat tccattatga aagcttctgc
     1261 tctcaagcag tttgttccga tacatttttt cttctgccct ggagatcacc tgctccatgt
     1321 ccatatctgt ctttttgtaa gcagaacagc ccgctgcaac actgatctta attggaaaac
     1381 tgacgatcag atcgagctca tatttgatac gtctcatcag attttccgca taagtacgat
     1441 tgtttctgac caataccaga aattcatctc cgccccatcg aacaggaata tccgcatcgt
     1501 cgcagcaata cgtaataata tcaccgatcg tctttaacag ccgatcccct tctctatgtc
     1561 cgaaggcatc attgatcagt tttaatccgt tcacatcagc catcagaata ctgaacacag
     1621 gatcctcttc ctgtttgtat tcttccagct tcttttccat ataggcacga tttttcaagc
     1681 ctgtcacagg atccgtctga agcaggaata ttttctgatt ctcattttct tttctttttg
     1741 taatatcgga aagacttccg acgaaccgca ctgcgtttcc ttccctgtca tattcaatga
     1801 tcccgcctct gacaaggacc cagatatatc catgctgcgc tttatacgca cgtacttctt
     1861 cgtaaaacag ttctccgccg ttttgaataa atgcatgcaa agcctcctca aatctctgtc
     1921 tgtcttccgg taagattcca tccagcactt taccgatatc cgtaaccaga tccagattat
     1981 tttcataacc cagatatttc agccatgtct ggctgatatc gatcgtctga tttgtgatat
     2041 cataatcaaa atacccgtca ttcgtcactt ccattgcatg gtagagatgc tcgttgttct
     2101 ttttgatcgc atcataattt ttttccagct ccatcagcat acagtttata ttttttccaa
     2161 gtaatgcgat ctcatccttc ccgcctacgg atattttggg aaaccggttt tctgtgaaag
     2221 agactgcctg tacggatttt gtcagatcaa cgatcctgct tatctctttt ttctccacca
     2281 taataataat aaacacggtc agaataccaa atacggcaaa cagcacggca ctttctgtct
     2341 tcagcatttg aacggcattg ctgtacgccg gtctcatgcc attaacttca aaagcaacgg
     2401 tattgtcatg cagaatatca tcctgagatt ctacgtcagt actttataaa ccaccctcaa
     2461 agcattgata aatata
//
LOCUS       DAOSYE010000307         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33142_length_2474_cov_3.152956, whole genome shotgun sequence.
ACCESSION   DAOSYE010000307 DAOSYE010000000
VERSION     DAOSYE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33142_length_2474_cov_3.152956"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLU43_06115"
     CDS             <1..749
                     /locus_tag="PLU43_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOO28022.1"
                     /translation="ILWVVLSPAFIINVLSSSMSPKTVRDYNGLIIAVMNRYFPKTKI
                     NTVLPKKQHVERYIPTADDIKKLVSIVNGTEMEVPVYLGAFAMMRRGEIAALNKSDIE
                     NNIIHVNKTMVYSDKNEWIIKSPKSYAGDRFIPVPTFVVDAFMALSTDGIEMTPNIIT
                     SRFEHVIRAAGLKHFRFHDLRHYCASIQHALGIPDAYIMQAGGWGDDRILKDVYRHTL
                     EDTKKKMSNVAISYFESMQHEMQHEIEKAP"
     gene            complement(767..837)
                     /locus_tag="PLU43_06120"
     tRNA            complement(767..837)
                     /locus_tag="PLU43_06120"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(803..805),aa:Trp,seq:cca)
     gene            complement(931..2361)
                     /gene="gatB"
                     /locus_tag="PLU43_06125"
     CDS             complement(931..2361)
                     /gene="gatB"
                     /locus_tag="PLU43_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOO28023.1"
                     /translation="MSRQYETVIGLEVHVELATKTKIFCGCSTAFGGAPNTHTCPVCT
                     GMPGSLPVLNRQVVEYAMAVGIATNCKITQYCKFDRKNYFYPDNPQNYQISQLYLPIC
                     RNGGVEVETAAGKKTVRIHEIHMEEDAGKLIHDEWEDCSLVDYNRSGVPLIEIVSEPD
                     MRSADEVIAYLEKLRLIIQYLGASDCKLQEGSMRADVNLSVREAGAPEFGTRTEMKNL
                     NSFKAIARAIEGERERQIELLEEGHAVVQETRRWDDNKEYSYAMRSKEDAQDYRYFPE
                     PDLAPVVISDEWIQEIKSRQPELRDEKRIRYKKEYDIPAYDIDIITGSKHMADIFEAA
                     VEICDSPKKVSNWLMVETLRLLKEKEMDPEDIKFSPENLAKLVTLTDNKVINSTVAKE
                     VFEIMFEKDIDPEKYVEEKGLKTVSDTGELQGVVEKVIADNPQSVADYRGGKEKAIGF
                     LVGQTMKAMKGKADPAVVNSLLKELL"
BASE COUNT          650 a          616 c          497 g          711 t
ORIGIN      
        1 caatactttg ggttgtatta tcaccggctt ttattatcaa tgttctttct tcaagtatgt
       61 ctcctaaaac agtcagggat tataacgggc tcataatagc ggtaatgaat agatatttcc
      121 ctaaaacaaa aatcaatacc gtattgccta aaaagcaaca cgtagaacga tatataccaa
      181 cagcggacga tataaaaaaa ctggtatcga ttgttaatgg aactgaaatg gaagtccctg
      241 tctatcttgg tgcatttgct atgatgcgta gaggtgaaat agctgcttta aataaatccg
      301 atatagagaa taatataata cacgtaaata aaacaatggt ctattccgat aaaaatgaat
      361 ggattataaa atcgcctaaa tcatatgccg gcgacagatt tattcccgtt ccgacgtttg
      421 tcgtggatgc atttatggca ctttctactg acggcataga aatgacaccc aatataatca
      481 catcccgttt tgagcatgta ataagagctg cggggcttaa acactttcgc tttcatgatc
      541 tacggcatta ctgcgcatca attcaacatg cacttggcat tcctgacgcc tatattatgc
      601 aggccggtgg ctggggagat gaccggatat taaaagacgt ttatcgtcat acccttgagg
      661 atacaaaaaa gaaaatgagt aatgtagcaa tcagttattt cgaaagcatg caacacgaaa
      721 tgcaacacga aatagaaaaa gcaccgtaaa tacggtgctt ttataacagg ggatgagaga
      781 atcgaactcc cactaaaagt tttggagact tctgtcatac cacttgacca atccccttta
      841 tacggcaggt cattttgacc tgcctattat ttatatcaca aacaaagact gcttgtcaac
      901 tataataagg aaactgcggg taaaagtatt ttacagcaat tccttcagca ggctgtttac
      961 aacagcaggg tctgctttgc ccttcattgc cttcattgtc tggcctacga ggaaaccgat
     1021 ggccttctct ttgccgcctc tgtaatcagc gacagactgc ggattatctg caatgacttt
     1081 ctcgacaacg ccctgcagtt cacccgtatc actgacagtc tttaagccct tttcttctac
     1141 atacttttca ggatcaatat ccttttcgaa catgatctca aagacttctt ttgcaactgt
     1201 ggaattaatg actttattgt cggtaagggt tacaagcttt gcaagattct ccggggaaaa
     1261 tttaatgtcc tccggatcca tttccttttc cttgagcagg cgcagcgtct cgaccatcag
     1321 ccagttgctg acctttttcg gactgtcaca gatctcaacg gccgcttcaa aaatatcagc
     1381 catatgtttg cttcccgtaa tgatgtcaat atcatatgcc gggatatcat attccttttt
     1441 ataacggatg cgcttttcat cccgaagctc cggctgtctg cttttgatct cctgtatcca
     1501 ttcgtccgaa ataacaacgg gtgcaagatc cggctccgga aaatagcggt aatcctgcgc
     1561 atcttccttg gaacgcatgg cataggaata ttccttatta tcatcccagc gtctcgtctc
     1621 ctgcacgacc gcatggcctt cctccagcag ctcgatctga cgttctctct ctccttcgat
     1681 cgcgcgcgcg atcgctttga acgaattcag gtttttcatt tctgttcttg ttccaaactc
     1741 cggcgcgccg gcttctctca ccgaaagatt gacatcggct ctcatggagc cttcctgcag
     1801 cttgcagtct gatgcgccaa gatactgaat gatcagacgc agcttttcca gataggcaat
     1861 cacttcgtcc gcgctcctca tgtcaggctc ggaaacgatc tcgatcagag gcactccgga
     1921 acggttgtaa tcaacaaggg agcagtcctc ccactcgtca tggatcaatt ttccggcatc
     1981 ctcttccata tgaatctcat ggatacggac cgtcttcttc cctgctgccg tttcgacttc
     2041 cacaccgccg tttcggcaaa ttggaagata cagctgagag atctggtaat tctgcggatt
     2101 atccggatag aaataattct tccggtcaaa tttacagtac tgtgtgatct tgcagttcgt
     2161 tgcgatccca accgccatcg catactctac gacctgcctg ttcagcaccg gaagggaacc
     2221 cggcatgccg gtgcagaccg gacatgtatg tgtattcggc gctccgccga aagcagtcga
     2281 acagccgcag aagattttcg ttttcgttgc aagttctaca tggacctcaa gtccgatcac
     2341 tgtttcatat tgtctgctca tcttctacgc ctccttttcg gactgttcat atgtataagc
     2401 cgcacgaatg atcttgtctt ccataaagca gtttccgatc agctgcatcc cgatcggcag
     2461 ccctgcctca cttt
//
LOCUS       DAOSYE010000308         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33150_length_2474_cov_2.675486, whole genome shotgun sequence.
ACCESSION   DAOSYE010000308 DAOSYE010000000
VERSION     DAOSYE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33150_length_2474_cov_2.675486"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..683)
                     /locus_tag="PLU43_06130"
     CDS             complement(12..683)
                     /locus_tag="PLU43_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO28024.1"
                     /translation="MEEHKLELHIDEYLPLRDVVFNTLRQGILTGELKPGERLMEIHL
                     ANRLGVSRTPIREAIRMLELEGLVTMIPRRGAEVARISKQDLRDVLELRRALDSLAIR
                     LACERITDEEKIALKEAALEFERSTKTKDATMIAQADVHFHDIILRASKNNRLIQTVN
                     HLAERVYRYRLEYIKDAKNHRRLIEEHAEILQCVGQGDAEAAQTASEAHIDNQEKNII
                     SQLDL"
     gene            complement(683..1558)
                     /gene="ispE"
                     /locus_tag="PLU43_06135"
     CDS             complement(683..1558)
                     /gene="ispE"
                     /locus_tag="PLU43_06135"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOO28025.1"
                     /translation="MEQINLKAMAKINLALDVIRKREDGYHEVRMIMQTLHLFDKLSI
                     KKVQKKGIFVKTNLYFLPTDENNLVYKAAKLLTEEFEIRQGVCINLQKYIPVAAGMAG
                     GSSDAAAVLFGLNRLFKLGLSTEQLMERGVRIGADVPYCIMRGTALSEGIGEILTPLP
                     PMPECFVLAAKPAVSVSTKFVYENLKIGELAKHPDIDGMTEAIKAQDLRGIAALMSNV
                     LETVTIREYPVIRQIKEHMMEGGALNALMSGSGPTVFGLFEEERTAWKAYRELKEKKL
                     AKQVFLTDIYNRRNL"
     gene            1801..2364
                     /locus_tag="PLU43_06140"
     CDS             1801..2364
                     /locus_tag="PLU43_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28026.1"
                     /translation="MNVNGISGYSQLDVYSTYANTGKETTSSETASSQDADTAVVYEK
                     TADADIVEMLKNDAAAREQSLMNMVQAAISGQGNALAQTDSVWSFLASGNFTVTEDVQ
                     QEAQESISEGGYWSVENTSDRIIEMAKALTGGDSDLIEEMRSAFEKGFQQATGLWGDT
                     LPDISSSTYDAVMEKFDQWAEESQTQI"
BASE COUNT          642 a          623 c          476 g          733 t
ORIGIN      
        1 cagtcactgc tttataagtc cagctgactg attatattct tttcctgatt atcgatatgt
       61 gcctcacttg ccgtctgtgc ggcttccgca tctccctgcc cgacacactg caggatctcg
      121 gcatgttctt ctatcagtct gcgatgattt ttcgcatcct tgatatattc cagccggtaa
      181 cgatatactc tttcggccag atgattcact gtctggatca gacgattgtt tttggaagct
      241 cttaatataa tatcgtgaaa atgtacgtca gcctgtgcga tcatcgtcgc atctttcgtt
      301 ttggtagacc gttcaaattc cagtgccgcc tccttcaaag cgatcttttc ctcatcagtt
      361 atgcgctcgc aggcaagacg gatcgcaagg gaatccagtg ctctccgaag ttccagcaca
      421 tcccgcagat cctgctttga tattcttgca acctccgctc cccgccttgg tatcatcgtc
      481 acaagacctt ccagctccag catccgaata gcctctctga tcggagtacg gcttactccc
      541 agacggtttg caagatgtat ctccatcaaa cgttctcccg gtttcagttc tccggtcagg
      601 atcccctgtc tgagcgtatt aaataccaca tccctaagcg gcagatattc atcaatatgc
      661 aattctaatt tatgctcttc cattatagat tcctcctgtt ataaatatcg gtaagaaata
      721 cctgttttgc aagtttcttt tcttttagtt ccctataagc cttccacgct gtcctctcct
      781 cctcaaaaag accaaatacg gtcggtccgc ttccgctcat cagcgcattc agtgcgcccc
      841 cttccatcat atgctctttg atctgtctga tcaccggata ctccctgatc gtcacggttt
      901 ccagcacatt cgacatcagt gcggcgatcc ctcttagatc ctgtgccttg atcgcttccg
      961 tcattccatc gatatccgga tgcttcgcaa gttctcctat tttcaaattt tcatacacaa
     1021 actttgtcga aacactgaca gccggcttcg ccgccaacac aaaacattcc ggcataggcg
     1081 gcagcggtgt caaaatctcg ccgatccctt ctgaaagtgc ggttccccgc ataatgcaat
     1141 aaggcacatc agcgccgatc ctaacgcctc tttccatcag ctgttccgta ctaagtccca
     1201 gcttaaacag acggttgagc ccgaacagta cggcggcagc atcagaactt ccgcctgcca
     1261 tacctgccgc aaccggaata tatttctgca gattgataca gacaccctgc ctgatttcaa
     1321 attcttcagt caaaagtttt gctgctttat aaaccagatt attttcatct gtcggcagaa
     1381 aatacagatt tgtcttcacg aaaatacctt ttttctgtac ttttttaata ctgagcttgt
     1441 cgaataaatg aagcgtctgc atgatcattc ggacttcatg atacccatct tcccttttcc
     1501 tgatcacatc caatgccaaa ttgatttttg ccattgcctt taaatttatc tgttccataa
     1561 aacgcattct ccccgactgt ttcagccctt gtattttgta taccgatcgt acttatctta
     1621 acatttttcg ttacagcttt tcaagggcgg ccgtgcaaaa ttttaacatt tctatttaaa
     1681 taaaatataa aatcatgtaa tgtattccta aaaaccgtcg atatatttat taagagttat
     1741 gattctattc aattgcggcg tcaggaaaac gccgcatcat taaccaagga gggttttact
     1801 atgaatgtaa atggaatttc aggatactca caactcgatg tctacagcac ctatgcaaat
     1861 acaggcaagg aaacgacttc gtcagaaaca gcttcttccc aggatgcgga tacggctgtt
     1921 gtttatgaaa aaaccgcaga tgcagatatt gtggaaatgc tcaaaaacga tgcggctgcc
     1981 cgtgaacaaa gtctcatgaa tatggtgcag gctgcgataa gcggacaggg gaatgcgctt
     2041 gcacagacag acagcgtatg gagtttctta gcaagcggaa actttaccgt aacagaagac
     2101 gtgcagcagg aagcacagga atccatttcc gaaggcggtt actggagtgt tgagaatact
     2161 tccgacagaa tcatcgaaat ggcaaaagca ctgacaggcg gcgattcaga cttgatcgaa
     2221 gaaatgcgga gtgcctttga aaaaggtttt cagcaggcga ccggactatg gggcgatact
     2281 cttccggata tttcttccag cacctatgat gctgttatgg aaaaatttga tcaatgggct
     2341 gaagaatccc agacgcaaat ataaagcttt gaaccatttc cggacaggat tttttgttac
     2401 taaaatcctg tccggaaaat ttttttttaa atttttttaa ttttcgtgtt tacattacgc
     2461 aggaaataat ctct
//
LOCUS       DAOSYE010000309         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33195_length_2472_cov_3.239553, whole genome shotgun sequence.
ACCESSION   DAOSYE010000309 DAOSYE010000000
VERSION     DAOSYE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33195_length_2472_cov_3.239553"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLU43_06145"
     CDS             complement(<1..234)
                     /locus_tag="PLU43_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO28027.1"
                     /translation="MRKGGVSDPRNKTLMKMFNLINIGERAGSGVPNIFNVWEDEGWI
                     EPAKKSGEKKWRKECDEENIRELQSNIRGYAARY"
     gene            complement(276..1274)
                     /locus_tag="PLU43_06150"
     CDS             complement(276..1274)
                     /locus_tag="PLU43_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOO28028.1"
                     /translation="MNELFDISKFDSYKEDNRREVKKANGGFPVTLWETYSSFANTYG
                     GVIILGIKELDDKSWKTTGLKTADKEKLLDDFWNLIHNSQKVNINLLSEKDVEVYEIG
                     QDLIIVIYVPMAKREQKPVFINSDMFGGTFRRDHSGDYHCTRLQVKAMLRDQTDNTMD
                     MEVLDDAKMAGLNQETIQGYRNRHRTLKAGHPFERLNDSGYLRSIGAAAISDVDKQLH
                     PTAAGMLMFGNEYNIVRHFPEYFLDYREVLDPTIRWTDRLQSGSGEWSGNICDFYFRV
                     YNKIIRDIKVPFKTKGGDRIDDTPVHEALREALANCLINTDFYGVRGVVIKKSQIS"
     gene            complement(1320..1769)
                     /locus_tag="PLU43_06155"
     CDS             complement(1320..1769)
                     /locus_tag="PLU43_06155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO28029.1"
                     /translation="MLQIKELCMEHIEEIKTFFVGIFTDEPWNDDWSDPVQLHAYMID
                     LIGNRNSFVLGLFENEAMIGLSIGSIKHWYSGTEYYIDELCIKKEKQGHGLGTRFLKE
                     IETFIKQKGMNQIFLQTKRTIPAYDFYKKNGYFELEDYVSMVRKLES"
     gene            complement(1772..>2472)
                     /locus_tag="PLU43_06160"
     CDS             complement(1772..>2472)
                     /locus_tag="PLU43_06160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOO28030.1"
                     /translation="EYQNGMIIQNILKCVPKVYGMAEIDDRSGIIYEEIHGTDMLKIM
                     AGVPWKINAYAKELAHDHLSIQKPVSSDLWTVKEKLEENLQNAKMLSNENKELVLKYL
                     KQLPDGNELCHFDFHPGNVMIADGRPVILDWMTACRGNACADVARTCLLLKYGEAVNV
                     PWITRKMISFFQHHIYKIYIKEYLAISLKSRKDIDSWELPVAAARLCEWVSENEKKKL
                     LWLVDGHCKKIRRG"
BASE COUNT          656 a          603 c          354 g          859 t
ORIGIN      
        1 atatctggct gcatagcctc taatattact ctgtagttct ctaatgtttt cttcgtcaca
       61 ttcttttcgc cacttttttt cgccactttt tttcgccggt tcaatccacc cctcatcttc
      121 ccatacattg aaaatatttg ggactccgct tcctgcacgt tcgcctatat ttataagatt
      181 aaacattttc attagtgttt tattgcgggg atctgacact ccaccttttc tcatctgagt
      241 ttttcctgtc ctgctataac ccgggttttc cataatcagc ttatctggct cttttttatt
      301 accacgcctc tgactccata aaaatccgta ttaatcaggc aatttgcaag ggcctctcgt
      361 aaagcttcat gcacaggtgt gtcatcaata cgatctccgc ccttagtctt aaatggtact
      421 ttaatatccc tgattatttt gttatatacc cgaaagtaaa aatcacagat atttcctgac
      481 cattcaccgg aacctgactg taatcgatcc gtccagcgaa tcgtaggatc aagcacttct
      541 ctataatcta aaaaatattc tggaaaatgt cttacaatat tatactcatt tccaaacatc
      601 agcatgcccg ccgctgtagg atgcagctgt ttgtccacat cagaaatagc tgctgcacca
      661 atgctacgaa gatatcctga atcatttaat ctttcaaatg gatgcccagc ttttaatgtc
      721 ctatgacgat tcctatatcc ctgaattgtt tcctgattta aacctgccat tttagcatca
      781 tctaacactt ccatatccat tgtattatcc gtctggtcac gcaacatcgc ctttacttga
      841 agtctcgtac aatgataatc tccggaatga tctcttctga atgttccgcc aaacatatca
      901 ctgttaataa atacaggctt ttgctcacgt tttgccattg gaacataaat cacaataatt
      961 aaatcttgtc caatttcata aacctccaca tccttttcag ataagagatt gatatttacc
     1021 ttttgactat tatgaatcaa attccagaaa tcatccaaca gcttctcttt atcagctgtc
     1081 tttaagcctg tagttttcca acttttatca tccagttctt taatacccaa aataattaca
     1141 ccgccataag tattggcaaa tgatgaatag gtctcccaga gcgtaactgg aaaccctcca
     1201 tttgcttttt taacctctcg gcgattatct tctttgtacg aatcaaattt tgaaatatcg
     1261 aataattcgt tcactttcga tttgctccct ttctccacgt catttaataa tcagatgcat
     1321 cagctctcta atttcctgac catggaaaca tagtcctcca attcaaaata tccgtttttt
     1381 ttataaaaat cataggcagg gatcgttcgt tttgtctgta aaaaaatctg attcattccc
     1441 ttttgtttta taaatgtctc gatttctttt aaaaaccgcg tacccagtcc gtgtccctgt
     1501 ttttcttttt ttatacagag ctcatcaatg taatactccg tgcccgaata ccaatgcttg
     1561 atgcttccga tagaaagtcc gatcattgct tcgttctcaa acagaccaag tacaaaggaa
     1621 tttcggttcc cgatcagatc gatcatatat gcatggagct gtaccgggtc gctccaatcg
     1681 tcattccacg gttcatcggt gaaaatacca acgaaaaatg ttttgatctc ttcgatatgc
     1741 tccatacaaa gttcctttat ttgcagcatt attatcctct cctgattttt ttacaatgtc
     1801 catctaccag ccatagcaat tttttcttct cattttccga gacccattcg cacaatctcg
     1861 ccgctgcaac aggcagttcc cagctgtcaa tatccttcct gctttttaac gatattgcaa
     1921 ggtattcttt tatatatatt ttataaatgt gatgctgaaa aaatgaaatc atttttcgtg
     1981 tgatccatgg tacgtttaca gcttccccat actttaaaag caggcatgtc cgtgcaacat
     2041 ccgcacatgc attccccctg catgctgtca tccagtccaa aataaccggc ctgccgtctg
     2101 ctatcattac atttcccgga tgaaaatcaa aatggcacag ctcattgccg tccggtaatt
     2161 gttttaaata ttttaaaaca agctctttat tttcattcga aagcatcttc gcattctgta
     2221 aattttcttc cagtttttct tttacagtcc acagatcact gcttaccggt ttttgtatcg
     2281 ataaatgatc atgcgccagt tcttttgcat aagcattgat tttccaggga acaccggcca
     2341 tgatcttcag catgtccgtc ccatggatct cctcatatat aatgcctgac ctgtcatcga
     2401 tctcagccat tccatatact tttggaacac atttcagaat attttgtata atcatgccgt
     2461 tctgatattc ct
//
LOCUS       DAOSYE010000310         2464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33361_length_2464_cov_3.339975, whole genome shotgun sequence.
ACCESSION   DAOSYE010000310 DAOSYE010000000
VERSION     DAOSYE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33361_length_2464_cov_3.339975"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLU43_06165"
     CDS             complement(<1..417)
                     /locus_tag="PLU43_06165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOO28031.1"
                     /translation="MEMSYFYIHKTQYYETDQMGIIHHSNYIRWFEEARTAFMEENGY
                     SYARLEAENVISPVLTAECEYRKMVHYGDTVKIEVIVEKFNGIKLVVGYRVFDLKTGE
                     LCTTGRTSHCFLNAAGRPISLKKEKEEFYRMFASALE"
     gene            640..1206
                     /locus_tag="PLU43_06170"
     CDS             640..1206
                     /locus_tag="PLU43_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOO28032.1"
                     /translation="MSLAMYLVDNIDTTELNRYRRSFYIGSILPDCKPSFLTTRHEFD
                     GTFQMIKDRILKLTTQAAQNDGNSCSYMRDLGQIIHYIADYFTYPHNTVYDGNLKDHC
                     IYEKHLKCSLREYIKSGEAARNQKQPKHFETPQALFGFIQKTHEDYLNMKHSVEEDCM
                     SIVRICHQVVISILQLFHMNINSIPVFA"
     gene            complement(1280..>2464)
                     /gene="serS"
                     /locus_tag="PLU43_06175"
     CDS             complement(1280..>2464)
                     /gene="serS"
                     /locus_tag="PLU43_06175"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO28033.1"
                     /translation="RLDEENRSAKQEADALRSSRNKISKQIGALMAQGRKEEAEQVKK
                     QVTANAKRLADLEEMEAQLSEKITGIMMSLPNMIDKSVPIGRDDSENVEVTKYGEPLV
                     PDFEIPYHTEIMERFDGIDLDAAGRVAGNGFYYLMGDIARLHSAVISYARDFMIDRGF
                     TYCVPPFMIRSNVVTGVMSFAEMDAMMYKIEGEDLYLIGTSEHSMIGKFIDTIIPEEQ
                     LPKTLTSYSPCFRKEKGAHGIEERGVYRIHQFEKQEMIVVCRPEESMMWFDKLWQNTV
                     DLFRSLDIPVRTLECCSGDLADLKVKSFDVEAWSPRQKKYFEVGSCSNLGDAQARRLK
                     IRVNSENGKYFAHTLNNTVVAPPRMLIAFLENNLNADGSVTVPQVLRPYMGNKEKLIP
                     VN"
BASE COUNT          676 a          639 c          427 g          722 t
ORIGIN      
        1 ttcaagagcc gatgcaaaca tccggtaaaa ctcttctttt tcctttttta atgagatcgg
       61 tctgcctgca gcatttaaaa aacaatggct tgttctgcct gttgtacaaa gctcccctgt
      121 tttcaaatca aaaacacggt acccgaccac aagtttgatc ccattgaatt tctcaacaat
      181 aacctcgatc tttacggtat ccccataatg taccatcttc ctgtattcgc actccgccgt
      241 aagcaccgga ctgatcacat tctccgcctc cagcctcgcg taggaatacc cattctcctc
      301 cataaaggcc gtacgggctt cctcgaacca tctgatataa ttagaatgat ggataattcc
      361 catctgatct gtttcataat actgtgtctt atgtatataa aaataactca tttccatctc
      421 cggcctgaaa tattgcacaa ttcattgctc tgttcatttt actctatttt gtttatattt
      481 acaacaagta agatattata caatttgttt tcaaaatctt cacagaatat ttactggact
      541 ttcgcgtccg gcaggaatat gattaatcac gaagtgaaaa atattgaaat cataattctt
      601 tgacagacaa aaggagtacg ctgtgagaaa aaatcacata tatcattagc aatgtattta
      661 gtcgacaata tcgatacgac agaattgaac agataccgca ggtcttttta tatcggaagc
      721 atcctgcccg actgcaagcc ttctttttta acgacacggc atgaattcga cggcaccttc
      781 cagatgataa aggaccgaat tttgaagctt acaacacagg cagcgcaaaa cgacggaaat
      841 tcctgctcct atatgcgtga cctcggccag atcatccact atattgcgga ttattttaca
      901 tatcctcata atactgttta tgacggaaat ctgaaggatc actgcatcta tgaaaagcat
      961 ctgaaatgca gtctccgtga atatatcaaa agcggtgagg cggcacgaaa ccagaagcag
     1021 ccgaagcatt ttgaaactcc ccaggcgctc tttggcttta ttcagaaaac tcatgaagac
     1081 tatttaaata tgaagcattc cgtagaagag gattgtatgt ctatcgtccg tatctgtcat
     1141 caggtcgtca tttctatttt acagctgttt catatgaata tcaattccat cccggtattt
     1201 gcataataaa taaaaaagag aacggccacg gatcatttcc gacagccttt ctctttttta
     1261 ttaccaggtc ccggctgttt tagttgaccg ggatcagttt ctccttattt cccatataag
     1321 gccgaagtac ctgtggaaca gtaacactgc cgtccgcatt gagattattc tcaagaaacg
     1381 cgatcagcat tcttggcgga gcaaccaccg tattattcag cgtatgcgca aaatattttc
     1441 cgttctcact atttacacgg atcttaagcc ttcttgcctg cgcatcccca agattggagc
     1501 agctgccgac ttcaaaatat tttttctgcc gcggagacca cgcctccaca tcaaacgatt
     1561 tcactttcag atcagccaga tcacccgaac agcattccag cgtccttacc ggtatatcaa
     1621 gtgagcggaa cagatcaacc gtattctgcc acagtttatc gaaccacatc atgctttctt
     1681 ccggtctgca gacaacgatc atctcctgtt tctcaaactg atggatacgg taaacacctc
     1741 tctcctcgat cccatgcgca cccttttctt ttctgaagca cggcgaatag ctcgtcaaag
     1801 ttttcggaag ctgttcctcc ggaatgatcg tatcaataaa cttaccaatc atggaatgct
     1861 cactcgtacc gatcagatac agatcctcac cttcgatctt gtacatcatc gcatccattt
     1921 cagcaaagct catcacgccc gttacaacat tgctgcggat cataaacggc ggcacacaat
     1981 aggtaaagcc cctgtcgatc ataaaatccc ttgcatagga gatcactgcg gaatgcagcc
     2041 ttgcaatatc acccatcagg taataaaatc catttccggc aactctgccg gcagcatcca
     2101 gatcgattcc gtcaaagcgc tccataattt ccgtatgata cggaatttca aaatcaggaa
     2161 caagcggttc cccatacttt gttacctcaa cattttccga atcatccctg ccgatcggta
     2221 cgcttttatc aatcatattc ggcagcgaca tcataattcc ggtaatcttt tcactaagct
     2281 gcgcttccat ttcttccagg tcagcaagcc ttttggcatt cgcagtcacc tgctttttta
     2341 cctgctctgc ttcttcctta cggccctgtg ccatcagagc cccgatctgt tttgaaattt
     2401 tatttctgct cgaacgcagt gcatcggctt cctgctttgc actcctgttt tcctcatcca
     2461 gtct
//
LOCUS       DAOSYE010000311         2464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33364_length_2464_cov_3.205479, whole genome shotgun sequence.
ACCESSION   DAOSYE010000311 DAOSYE010000000
VERSION     DAOSYE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33364_length_2464_cov_3.205479"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLU43_06180"
     CDS             <1..466
                     /locus_tag="PLU43_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOO28034.1"
                     /translation="LCSSVGETVDAMQDFAKYSGVPIVRVDEEMCREAVRRGKVIGVL
                     ATLRTTLEPTQNTIKRAAREAGKQVTLVPGLFDAFGADPEKFNQLMLEAGAKMKDSVD
                     VIVLCQGSMAFGEKVLRDALHIPVLSSPGFGAVELKKALQRKGLETSGGTLC"
     gene            460..2427
                     /locus_tag="PLU43_06185"
     CDS             460..2427
                     /locus_tag="PLU43_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOO28035.1"
                     /translation="MLTDTRTSKYVKVTAPEWDQVKWTGGLWGETAATCYHHTVFQLK
                     RMFDDKTISHVVENFRICAKETDGAHDGTVFGDGDFYKWMEAALYTAAENRDEALFSE
                     LDAYIDLIGRAQQPDGYLSTKQIIGERSNNGVARMGDIDDFEVYNFGHLFTSACLHKR
                     VTGKDSFLKIAVKAADYLEKLYDEAEKRGEVQTAVCPSHYMGLIELYRTTGQEKYLAL
                     ARKAIELRDSVKDGKDDNQDRLPLKQHEKIIGHAVRANYLYAGVADLYAETGDPKYLQ
                     VLHKVWNDLMSYKIYLTGGCGALYNGASPYGNFFVHQLIHQAYGYEYQLPNITAYAET
                     CASVGLVMWAYRMFLIEPEAKYFDAIERTMLNVNLAALSLDGKKFFYENMLRRTKKLE
                     YEMVWPLARTEYITSYCCPPNLARVLSQSAEYAYMKSEDALWLGMYGANEAVLSLACG
                     TSFQVKQKTEYPFSGEIILEFSEVSGPSTVTVMVRIPGWAETGRITVSDGRVYEVDQK
                     NAGSYLPVRVSLRNGLRLELQFDMPVRLTAANAMVEENTNQAAVERGPLVYCMETEDA
                     SVETLDDVYLRADGKFSMEPYEIDGHQMTALIGDAYVMKRESYDRTALYQTFKFHGME
                     KTKIRMIPYYAWDNRGFQEMRIWMPLVYPCE"
BASE COUNT          684 a          483 c          704 g          593 t
ORIGIN      
        1 tttatgttct tctgtcgggg aaacggtgga tgccatgcag gattttgcta aatacagcgg
       61 ggtgccgatc gtacgcgtcg atgaagaaat gtgccgtgag gcagtcagac gcggaaaggt
      121 gatcggcgtg cttgcaacac tgcgcacgac actggaaccg acacaaaata caatcaagcg
      181 ggcagcacgc gaggccggaa agcaggtgac gctcgttccg ggactgtttg atgcgtttgg
      241 ggcagatccg gaaaaattca accagttgat gctggaggcc ggagcaaaaa tgaaggacag
      301 tgtggatgtg atcgtattat gccagggctc catggctttt ggagaaaagg tgctgcgcga
      361 tgcactgcat atacccgtat tgtcaagccc gggctttggt gctgtggaat tgaaaaaagc
      421 gctgcagagg aaaggcttgg aaaccagtgg aggaacatta tgttaaccga tacaagaact
      481 tccaaatatg taaaagtaac ggcgccggaa tgggatcagg taaaatggac gggcggttta
      541 tggggtgaaa ctgcagctac ctgttatcat catacggttt tccagctgaa gcgcatgttt
      601 gatgataaaa cgatcagcca cgtagttgaa aattttcgta tttgtgcaaa ggaaacagac
      661 ggtgcgcatg acggaacggt tttcggagac ggagattttt ataaatggat ggaagccgca
      721 ttatatacgg cggccgaaaa cagggatgag gcactgtttt cagaactgga tgcctatatc
      781 gatctgatcg gccgagcaca gcagccggac gggtatcttt ccacaaagca gatcattggg
      841 gaacgcagca ataatggtgt tgcaaggatg ggtgacatcg acgattttga agtatataat
      901 tttggccatt tatttacttc tgcctgtctg cataaaaggg taacagggaa agacagtttt
      961 ttgaagatcg cagtaaaagc agcggattat ctggaaaagc tttatgacga agcggaaaaa
     1021 aggggagagg tgcagacggc agtctgtcct tctcattata tgggcctgat cgaactgtac
     1081 cgtacaacgg ggcaggagaa ataccttgca cttgcaagga aagcaatcga attgagagat
     1141 tccgtaaaag acggcaaaga tgataatcag gatcgtctgc cgcttaaaca gcatgaaaag
     1201 atcatcggcc atgctgtcag ggcgaattac ctgtatgccg gtgttgccga tctgtatgcg
     1261 gaaacaggtg atccaaaata tctgcaggtg ctgcataagg tttggaacga tcttatgagt
     1321 tataagatct atttaacagg cggatgcggg gcgctttata acggggcgtc tccttatggc
     1381 aatttttttg tgcatcagct gatccatcag gcatacggat atgaatacca gctgcctaat
     1441 attaccgcat atgccgagac atgtgccagt gtcggccttg tgatgtgggc atacaggatg
     1501 tttctgatcg aaccggaagc aaaatatttt gatgcaatag aaaggacgat gctgaatgtc
     1561 aatctggcgg cactgagtct tgacggtaaa aaattttttt atgaaaacat gctcagaaga
     1621 acaaagaagc tggaatatga gatggtctgg ccgcttgcac gtacggaata cattacgtct
     1681 tactgctgtc cgcccaatct tgcgcgggtg ctgtcacaga gtgcggaata tgcgtatatg
     1741 aaatccgagg atgcgctctg gcttgggatg tacggcgcga acgaagcggt cctttctctt
     1801 gcatgcggca cttcttttca ggtaaaacag aaaacagagt atcctttttc aggagagatc
     1861 atcctggagt tttctgaagt ctcggggcca tcgaccgtta cggtcatggt aaggatcccg
     1921 ggttgggccg aaaccggccg tataacggtt tctgacggca gggtatatga agtggatcaa
     1981 aaaaatgccg gaagctatct gcctgtccgg gtgtctttgc gaaacggcct gcggctggag
     2041 cttcagtttg atatgccggt gaggcttacg gcggcaaatg ccatggtaga ggaaaacacg
     2101 aaccaggcgg cagtagagcg cggaccgctt gtatactgca tggagacaga ggatgcctcc
     2161 gtagagacgc tggatgacgt ttatctgcgt gcggacggaa aatttagtat ggaaccctat
     2221 gagatcgacg gtcatcagat gacggcactg atcggagatg cgtatgttat gaaacgggaa
     2281 tcctatgaca gaacggcatt gtatcagaca ttcaaatttc atggcatgga gaaaacaaaa
     2341 atccgcatga tcccttatta tgcatgggat aaccgcggat ttcaggaaat gcgtatctgg
     2401 atgcctctgg tgtatccatg tgaataaggg agatcgatca tgaatgctgt attggaacga
     2461 ttat
//
LOCUS       DAOSYE010000312         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33506_length_2458_cov_2.995839, whole genome shotgun sequence.
ACCESSION   DAOSYE010000312 DAOSYE010000000
VERSION     DAOSYE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33506_length_2458_cov_2.995839"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLU43_06190"
     CDS             complement(<1..327)
                     /locus_tag="PLU43_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28036.1"
                     /translation="MEKNILYIQSNEDVAESFREKFAERGIELIVAASASEAFQVMRE
                     REITLLLIDINIPDMRLRNLVEVCTRDFPGVILNVCVDVLSPLLITKLVNRHRIHKIF
                     VAPWNVD"
     gene            complement(358..>2458)
                     /gene="htpG"
                     /locus_tag="PLU43_06195"
     CDS             complement(358..>2458)
                     /gene="htpG"
                     /locus_tag="PLU43_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOO28037.1"
                     /translation="QGNLSINSDNIFPIIKKWLYSDHDIFVREMISNGCDAITKLKKL
                     DMMGDYSFPDEHENKIRVIVNPEEKTLKFIDTGIGMDADEVEEYITQIAFSGATAFLE
                     KYKDKTNEDQIIGHFGLGFYSAFMVADDVHIDTLSYKEGAKPVHWECDGGTEYTLDEG
                     TKTDVGTEITLFLNEESVEFCNEYRAREIIKKYCSFMPIPIYLEKANAEPEYETIDVE
                     EKNEKDTVIETIHEEAKYEEKENEDGTKEQVEVSPAKDKLKILKRPVPLNDTTPLWTK
                     HPNDCTKEEYLEFYRKVFLDYKEPLFWIHLNMDYPFNLKGILYFPKINMEYESIEGTI
                     KLYNNQVFIADNIKEVIPEFLMLLKGVIDCPDLPLNVSRSALQNDGFVKKISDYISKK
                     VADKLSGMCKTEKEEYEKYWDDISPFIKYGCLKDEKFCDKITDYILFKNLEGNYVTLP
                     ECLELEKKTEAEATDENGEKVEAEIVEEKPEDTGAETDDTEEEEKPKKVIYYVTDEKQ
                     QSQYINMFREQGMDAVILTHNIDQPFVSQLESKNEGIKFQRIDADLTDSFKEEAAEGD
                     KEAIEEKTTTLSELFRKVLGKDKLEVKVEKLKNGNVSSMLTVSEESRRMQDMMKMYNM
                     SGMDMGAFGTEGETLILNANNKLVQYIFENKDSENVPIICEQLYDLAVLSNKPLSPEA
                     MTKFVARSNEIMLLLAK"
BASE COUNT          626 a          637 c          409 g          786 t
ORIGIN      
        1 gtcaacattc cacggcgcta caaagatctt atggatccga tgcctgttca caagcttcgt
       61 gatcagcagc ggacttaaca catcaacaca gacatttaat attacgccgg gaaagtcgcg
      121 cgtacacact tccaccaaat ttcgaagccg catatccgga atattgatat cgatcagcag
      181 cagcgtaatt tcacgctcac gcataacctg aaaggcttcg gaagcagacg ccgcaacgat
      241 cagttcaata ccccgctcgg caaacttttc ccgaaaactt tcagcaacat cctcattaga
      301 ctgtatgtaa aggatatttt tttccataac ggctcccacc cacaaatatg ccacaaatta
      361 tttagctaat aataacatga tttcattgct tcttgcaaca aatttagtca tggcttccgg
      421 tgaaagcggc ttattggaaa gcactgccag gtcgtagagc tgttcacaga tgatcggaac
      481 attttcactg tccttatttt caaaaatata ctgtaccagc ttattgtttg catttaagat
      541 cagcgtttcg ccttcggttc cgaacgcgcc catatccatg cccgacatat tatacatttt
      601 catcatatcc tgcatacggc ggctttcttc agaaactgtc agcatggaag atacgttccc
      661 gttcttaagc ttttccactt taacttccag cttatccttg ccaagcacct ttctaaacag
      721 ttcggaaagc gttgtcgttt tctcctcgat cgcttccttg tctccttccg ctgcttcctc
      781 cttaaaggaa tcggtcagat cggcatcgat cctctggaac ttgatgcctt cgttctttga
      841 ctcaagctgg gatacaaacg gctgatcgat gttatgtgtc agaatgacgg catccatccc
      901 ctgttccctg aacatattga tatactgaga ctgctgtttt tcatcagtaa catagtaaat
      961 gacttttttc ggcttctctt cctcttccgt atcatctgtt tcggctccgg tatcctccgg
     1021 cttctcttca acgatctcag cttctacctt ttcaccgttc tcatccgtgg cctctgcctc
     1081 tgttttcttt tcaagctcca gacattccgg cagcgttaca tagttgcctt ccagattctt
     1141 aaagagaata taatccgtta tcttatcaca gaatttctca tcctttaagc agccgtattt
     1201 aataaacgga ctgatatcat cccagtattt ttcatactct tccttttcgg tcttgcacat
     1261 gccggacagc ttgtccgcaa cctttttgga aatatagtct gagatcttct tcacaaaacc
     1321 atcgttctgc agcgcgcttc tggacacatt cagcggcagg tccggacagt cgatcacgcc
     1381 ctttaaaagc atcagaaatt ccggaatgac ttctttgata ttatccgcaa taaacacctg
     1441 attattatat aacttgatcg tgccttctat cgactcgtat tccatgttga tcttcgggaa
     1501 atacaggatc ccttttaaat tgaacggata atccatgttc agatggatcc agaacagcgg
     1561 ctccttataa tctaaaaata cctttctgta gaactccaga tattcttcct ttgtacagtc
     1621 attcggatgc tttgtccaaa gaggagtcgt atcgttcaaa ggcaccggac gcttcagaat
     1681 tttcagtttg tcctttgccg gagaaacctc aacctgttcc tttgtaccat cctcattttc
     1741 tttttcctca tacttggctt cttcatggat cgtctcaata acagtatctt tttcattctt
     1801 ttcttcaacg tcgatcgtct catattccgg ctccgcattt gctttttcaa gatagatcgg
     1861 aatcggcata aaggaacagt atttctttat gatctctctc gcacggtact cattgcagaa
     1921 ttccacgctc tcctcattca aaaacagcgt gatctccgta ccgacatctg tctttgttcc
     1981 ttcatcaagc gtatattccg taccgccgtc acattcccag tgaaccggtt ttgcaccctc
     2041 tttataggaa agcgtatcga tatgaacatc atccgctacc ataaatgcag aataaaagcc
     2101 aagtccgaaa tgcccgatga tctgatcctc gttcgtcttg tctttatatt tctccaaaaa
     2161 tgcggttgca ccggaaaacg cgatctgcgt aatatattct tccacttcat ccgcatccat
     2221 accaataccg gtatcaataa atttcaatgt tttttcttcc ggattcacaa tgaccctgat
     2281 cttattctca tgttcgtcag gaaaagaata atctcccatc atatcaagct ttttcagctt
     2341 tgtgatcgcg tcacatccat ttgagatcat ttctcttaca aaaatgtcat ggtcagaata
     2401 taaccatttt ttaataatcg gaaaaatatt atcactgttg atcgataaat tcccttgc
//
LOCUS       DAOSYE010000313         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_33716_length_2449_cov_2.793233, whole genome shotgun sequence.
ACCESSION   DAOSYE010000313 DAOSYE010000000
VERSION     DAOSYE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33716_length_2449_cov_2.793233"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1725)
                     /locus_tag="PLU43_06200"
     CDS             complement(88..1725)
                     /locus_tag="PLU43_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28038.1"
                     /translation="MKRKIAATILSVCMVLGLAGCGADAQSQTGETVVTDEEAAEETV
                     VTDEEAEEETAGAETEIEEETTEPVTIRLEGSNISTPNPFRHTTRGPGIFRTWLLYDS
                     LLEKDENGDIPWLAKDWDVSEDGTVYTFYLEEDACWHDGEPLTAEDVAFTFDYYREHL
                     PVYHSLLADGEYIVDKTEAIDDHTVEITLTHFDNTFLSSIGMARIIPKHIWENIEDPA
                     AYDGEGVTVGSGPYMLDSYDAEQGAYRYVAFEDYWGLTPAAEAIEWVPVSDSVLAFEN
                     EEIDLLNASADLLSRYENDSQYTVKSVSSLHSFRLMMNMEAVPEFLDVNVRKAIAYAI
                     NCQELIDTVSRGAGTVSSMGYVPMESAWYNSDTVQYDYDPQKAEELLDGQTYSFKLLT
                     DNSADSTKTAEMIKIYLAEVGIDVTIESVESKTRDNAITTGEYELLLNYIGGMGGDPD
                     YLRDVYGQEAGTIRGWSNETVAELLTAQAVEQDTAAREEMIDEVQEIISDEVPMIMLY
                     GKVVNYVYRQDKYDGWMCRYDHNMLDHNKLSYLIREE"
     gene            complement(1754..>2449)
                     /locus_tag="PLU43_06205"
     CDS             complement(1754..>2449)
                     /locus_tag="PLU43_06205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO28039.1"
                     /translation="IDHAGKVAGLLLEKGIIKDADVLDIGGGTGRYAVPFAEYAKEVT
                     IADVSSQMMAYAKENAEKVDRRNLQYVRLNWEEADLKELGWEKRYDLVFASMCPAVMS
                     GAGIEKMSAASRGRCQINQLFEMTDNISRRLAEELKIERNYDPHNDRDTVQGIFNVLW
                     LQGLEPEITYLRDTKQQVLSVDEALPQYSHRFGKAALEKGIEIKSLLEQYAGGKDLTI
                     ESSAALAMICWKV"
BASE COUNT          595 a          687 c          457 g          710 t
ORIGIN      
        1 gaacggatcg ccgggcatca gccgcggcaa agtaaaatta attaccagaa ttataaaaag
       61 tacaagcaga taatgctttt tcatctgtta ttcttccctg atcaaataag acagcttatt
      121 atgatccagc atattgtgat cataccggca catccagccg tcatatttat cctgacgata
      181 cacgtagttc acaacttttc cgtacagcat gatcatcggc acctcatcgg aaataatctc
      241 ctgtacttca tcgatcattt cttcccttgc tgcagtatcc tgctcaactg cctgtgcagt
      301 caaaagctct gcaaccgttt catttgacca gccgcgtatc gttcctgcct cctggccgta
      361 aacatcacgc agataatccg gatcaccgcc catgccgcct atataattaa gaagcagttc
      421 atattctcct gtcgtaatcg cattgtccct cgtcttgctt tccacgcttt cgatcgttac
      481 gtcgattcct acttcggcaa gatagatctt gatcatttca gccgtctttg tcgaatcggc
      541 agaattgtcc gtcagcagtt tgaaggaata ggtctgacca tcaagcagtt cctcagcctt
      601 ttgcgggtca tagtcatact gcaccgtatc ggaattatac cacgcactct ccattggaac
      661 atatcccatg ctgctgacag tacctgcacc gcgcgaaacc gtatcaatca gttcctggca
      721 attgatcgca tatgcgatcg cttttctgac attgacatcc aaaaattccg gaacagcttc
      781 catattcatc attaagcgga agctgtgcag ggatgaaaca cttttcaccg tatactgact
      841 gtcattttca taacgggaca gcagatctgc agaagcattg agcagatcga tctcttcatt
      901 ttcaaatgcc agtacgctgt cgctgaccgg tacccactcg atcgcctcag ccgcaggagt
      961 aagtccccag tagtcttcaa atgcaacata ccggtatgca ccctgctctg catcataact
     1021 atccagcata tacggaccgc ttccaacagt aacgccctcg ccgtcataag ccgccggatc
     1081 ctctatattt tcccatatat gctttggaat aatgcgggcc ataccgatac ttgaaagaaa
     1141 cgtgttgtca aaatgcgtaa gtgtgatctc taccgtatga tcatcgattg cctctgtttt
     1201 atcaacaata tattccccgt ctgcaagcag gctgtgatag accggcagat gctctctgta
     1261 gtaatcaaac gtaaatgcca cgtcttccgc tgtcaaaggc tctccgtcat gccagcatgc
     1321 atcttcctct aaataaaaag tataaacagt gccgtcttcg cttacatccc agtcttttgc
     1381 aagccacgga atatctccgt tttcatcttt ttcaagaagt gaatcatata agagccatgt
     1441 cctgaaaata ccgggacctc ttgtcgtatg ccggaacggg ttcggcgtac tgatattact
     1501 gccttccaga cgaattgtga ccggttcggt cgtttcttct tctatttccg tttctgcacc
     1561 agcagtctct tcttctgctt cttcatctgt aacaacagtc tcttctgctg cctcttcatc
     1621 tgtaacaaca gtctccccgg tctgtgactg ggcatctgct ccgcagccgg ccagtccaag
     1681 caccatgcag accgataaaa ttgttgctgc aatctttctt ttcataatct tcctcctgtt
     1741 ttataaatgc aaactatact ttccagcaga tcatggcaag tgccgcactg ctttctattg
     1801 ttagatcttt gccgccggca tactgctcca gcagactttt gatttcaatt cctttttcca
     1861 aagcagcctt gccaaaccga tgggaatatt ggggcagcgc ttcatcaaca gacaaaacct
     1921 gctgctttgt atctcttaaa taagtaatct ccggctcaag tccctgcagc caaagcacat
     1981 tgaaaattcc ctgtaccgtg tccctgtcgt tatgaggatc atagtttctt tcgatcttca
     2041 gttcctctgc cagcctccgg gatatattat ccgtcatttc aaacagctgg ttaatctggc
     2101 accggcctcg tgatgcggcc gacatctttt caattccggc tccgctcatt acggcaggac
     2161 acatggatgc aaaaaccaga tcataacgtt tttcccagcc cagctccttc agatcggctt
     2221 cttcccaatt aagcctgaca tactgcaggt tccggcggtc cactttttca gcattttctt
     2281 tcgcatacgc catcatctga gaagagacat ccgcaatcgt gacttccttt gcatattctg
     2341 caaaaggaac cgcatatcgc cctgtccctc cgccgatatc cagaacatcg gcgtctttta
     2401 ttatgccttt ttctaacagc agcccggcta ctttccctgc atgatcgat
//
LOCUS       DAOSYE010000314         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34098_length_2431_cov_3.306818, whole genome shotgun sequence.
ACCESSION   DAOSYE010000314 DAOSYE010000000
VERSION     DAOSYE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34098_length_2431_cov_3.306818"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLU43_06210"
     CDS             <1..515
                     /locus_tag="PLU43_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO28040.1"
                     /translation="RCTELHMRPVPDKDVRKYLMETLHVPDYQADICIAFARGNIGKA
                     KSLAMSEEFENIKREAITVLKYIHEMDIGELAQSIKKLNEYKVSISDYLDIMIIWYRD
                     VLLFKATNDANDLIFKEEVKYIRERANQSAYEGIETIIQAIKKTKIRLEANVNFDLAI
                     ELLMLTIKEN"
     gene            517..1425
                     /locus_tag="PLU43_06215"
     CDS             517..1425
                     /locus_tag="PLU43_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOO28041.1"
                     /translation="MTKVIGIRFRTAGKVYYFDPKKLNFKRGDHVIVETARGVEYGTV
                     VMPPTNVEDEKVIQPLKPVIRKATEDDAKTEQKNREKEKEAFQICLEKIQKHKLEMKL
                     IDAEYTFDNNKVLFYFTADGRIDFRELVKDLAAVFKTRIELRQIGVRDETKILGGVGI
                     CGRPLCCHTYLSDFIPVSIKMAKEQNLSLNPTKISGICGRLMCCLKNEEDTYEELNRK
                     LPNVGDFVTTDDGLKGSVSSVNVLRQLVKVIVDVGDEKEIKEYKAGQLRFKSKHKKEK
                     AKLSAEELKELARLEKQDGKSKLDDN"
     gene            1397..2152
                     /locus_tag="PLU43_06220"
     CDS             1397..2152
                     /locus_tag="PLU43_06220"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOO28042.1"
                     /translation="MENQSSTTIKLKEHEHIDELNRNGYRIIQNEKTFCFGMDAVLLS
                     GFAKVKEDETVLDIGTGTGIIPILLEAKTAGKHFTGIEIQEEIADMARRSVALNGLSH
                     RIEIVTGDIKEAERLFAMSSFDVITTNPPYMIGSHGLKNEAESKAIARHEILCTLEDI
                     VREGSRLLKPGGRFYMVHRPFRLVELFAVLTKYRLEPKRMRLVYPYADKEPNMVLIEA
                     LYGGRSRITVEKPLVVYKERGKYTDEIYDVYGY"
     gene            2177..>2431
                     /locus_tag="PLU43_06225"
     CDS             2177..>2431
                     /locus_tag="PLU43_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOO28043.1"
                     /translation="MQEKIPQGMLYLCATPIGNLSDMTFHVLDTLQKVDLIAAEDTRN
                     SRKLLNYFKIKTPMTSYHEHNKYDKAADPIRQMQEGKSVAL"
BASE COUNT          824 a          390 c          632 g          585 t
ORIGIN      
        1 ccagatgtac ggaattgcat atgcgtccgg tcccggataa agatgtccgg aaatatttaa
       61 tggaaacgct gcatgtgccg gattatcagg cagatatttg tattgctttt gcaagaggaa
      121 atattgggaa ggcaaaaagc cttgcaatga gcgaagagtt tgaaaatata aaaagagaag
      181 cgattactgt gcttaaatat attcatgaaa tggatattgg ggagctggca cagtcgatca
      241 aaaaactaaa tgaatacaaa gtcagtattt ccgactatct ggatattatg atcatttggt
      301 atcgtgatgt tctgcttttt aaagcaacga acgatgcgaa tgaccttatt tttaaagaag
      361 aagtcaaata tatcagagaa cgtgcaaatc aaagcgcata tgaaggaatc gaaacgatca
      421 ttcaggcaat caagaaaaca aaaatcagac tggaagctaa tgttaatttt gatttggcaa
      481 tcgaacttct gatgcttaca ataaaggaga attagaatga caaaagtaat aggaatcaga
      541 ttcaggacag caggaaaggt ttattatttt gatccgaaga agttgaattt taaaagaggc
      601 gaccatgtga tcgtggaaac cgcaagaggc gttgaatatg ggacggtcgt aatgcccccg
      661 acaaatgttg aggatgaaaa ggttattcag ccgctgaagc cggtaatcag aaaggcaacg
      721 gaggacgatg caaaaacgga acaaaaaaac agagagaagg aaaaagaggc ttttcagatc
      781 tgtcttgaaa aaatacagaa gcataagctt gaaatgaagc tgattgacgc ggagtatacg
      841 tttgataata ataaagtgct gttttacttt acggcagacg ggcgcattga ttttcgcgaa
      901 cttgtaaagg accttgccgc agtgtttaaa acaaggatcg agctgcggca gatcggtgtc
      961 agggatgaga caaaaatcct gggaggtgtc ggtatttgcg gacggccttt atgctgccat
     1021 acatacttat cggattttat tccggtatcg atcaaaatgg caaaagagca gaacctgtct
     1081 ttaaatccga cgaagatctc cggtatttgc ggcagactga tgtgctgttt gaaaaatgaa
     1141 gaggatacgt atgaggaatt aaaccggaaa ttgccgaatg tcggtgattt tgtaacaacg
     1201 gatgatgggt taaagggttc cgtatccagc gtaaatgtat taagacagct tgtcaaagta
     1261 atcgttgatg ttggtgatga aaaggaaatc aaagaatata aggcagggca gcttcgattt
     1321 aaatcaaagc acaaaaaaga gaaggcaaag ctttctgcgg aggaattaaa ggaactggcg
     1381 cggctggaga agcaggatgg aaaatcaaag ctcgacgaca attaaattaa aagaacatga
     1441 acacattgat gaactgaacc ggaacggcta ccggatcatt cagaatgaaa aaactttttg
     1501 ctttggcatg gacgctgttt tgctgtccgg gtttgcaaag gtaaaagagg atgaaacggt
     1561 tctggatatc ggaaccggga cggggatcat accgatcctg ctggaagcga aaacagccgg
     1621 aaaacatttt acgggaattg aaattcagga agagattgcg gatatggcac gaagaagtgt
     1681 ggcgctgaat ggactttccc atcgcataga gatcgttacc ggcgatataa aggaagcaga
     1741 aagactgttt gcgatgtcgt cttttgatgt gataacaaca aatccgccgt atatgatcgg
     1801 cagccatggt ctgaaaaatg aagcagagtc aaaagcgatt gcgcggcatg aaatattatg
     1861 tacacttgag gacattgtca gggaaggcag caggctgtta aaacctggcg gcagatttta
     1921 tatggtgcat cggccgttcc ggcttgtgga gctatttgca gtacttacga aatatcggct
     1981 ggaaccaaaa agaatgcggc ttgtgtatcc gtatgcggat aaagaaccca atatggtgct
     2041 gatagaagcg ctgtatggag gaagatcaag aataactgtg gaaaagccgt tggttgttta
     2101 taaggaacgc ggaaaatata ccgacgaaat ttatgatgtt tacggatact gaggctgctg
     2161 atgggaggaa gaggaaatgc aggagaaaat tccgcagggc atgctgtact tatgtgcgac
     2221 tccgatagga aatctcagtg atatgacgtt tcatgttctt gatacattgc agaaggttga
     2281 tctgatagca gcggaagata cgcgcaacag cagaaagctg ttaaattatt ttaaaataaa
     2341 aactccaatg acaagttatc atgaacataa caaatatgac aaagccgcgg atccgatccg
     2401 gcagatgcag gaagggaaaa gcgttgcatt g
//
LOCUS       DAOSYE010000315         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34223_length_2426_cov_3.306622, whole genome shotgun sequence.
ACCESSION   DAOSYE010000315 DAOSYE010000000
VERSION     DAOSYE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34223_length_2426_cov_3.306622"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..878)
                     /locus_tag="PLU43_06230"
     CDS             complement(147..878)
                     /locus_tag="PLU43_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOO28044.1"
                     /translation="MMETKKLEKYFGHLEVLKGIDFSIEKGEVVSLIGSSGSGKSTFL
                     RCLNFLENKTSGDILYMDKPIEKHSSEINELRKKIGMVFQDFNLFLNMTILDNVMSGP
                     VIVLKKSKEEARRIALEMLEKVGLSEKAAAYPIALSGGQQQRVAIARALAMSPEVILF
                     DEPTSALDPELVVEVLNVMKEMAKEGMTMIIVTHEMGFAKEVSDRVIFLHEGVIAEQG
                     TAEDIFLRPQNERLRSFLKSMEILS"
     gene            complement(881..1552)
                     /locus_tag="PLU43_06235"
     CDS             complement(881..1552)
                     /locus_tag="PLU43_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOO28045.1"
                     /translation="MFEGIMNFFVIAVENMPKFRGAIGNTLLLSILSIIFGVVIGIIV
                     NLMTMTKIKPLVWIAKFYIAVIRGTPLLLQLFFVFYGLPQIGIYFSSFASAVLGLAFH
                     NGAYISEIFRGAILAVPHGQNEASRAIGMTKLQAFRHVIFPQAFKHAVPALGNQFLVS
                     VLDSSLASVITITETMMKARQLAAATYSIFPIYFDAALFYLLMTYVLSFFLKKLENRL
                     KVNER"
     gene            complement(1659..2342)
                     /locus_tag="PLU43_06240"
     CDS             complement(1659..2342)
                     /locus_tag="PLU43_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO28046.1"
                     /translation="MQKEGKFTFACSGGYPPFNYIDEDGSLIGFDVEIAQALGEEMGV
                     EAEGITTDWDGILGGLTGKRFDTIIGSMAITDERLEQVNFTDPYYYDGSQFFSPSGLG
                     LTSIADAAGSGVGVVTGTTYQTFLEENYPDVEVVPFSSDIDNFMAASEGRTVGLVTSR
                     FVGLQAPEEYDLVPTGELLYAENIGIAVRKEDTELLENLNNALATIVENGTYEEISMK
                     YFGTNILEK"
BASE COUNT          692 a          585 c          421 g          728 t
ORIGIN      
        1 agtggtggaa acattgtaac tggggctagc cgtcgcgcta gcgacttggt acaatgggat
       61 aatccttttt aaaaaaagga tttaaaatgt atgtattttt actcatttcc atatctcttt
      121 tcttattagg ttaaatttat ttccattcag gataagatct ccatactttt taaaaatgat
      181 cttaaacgtt cattttgagg acgcagaaaa atatcttccg ctgttccctg ttctgcaatc
      241 acaccttcat gcagaaaaat aactctgtct gatacttcct ttgcaaaccc catttcatgc
      301 gtgacaatga tcatcgtcat cccttccttt gccatttcct tcatgacatt cagcacttca
      361 acgaccaatt cgggatcgag cgcggacgtc ggctcatcaa aaaggatcac ttccggagac
      421 atggccaatg cgcgtgcaat tgcaaccctt tgctgctgac ctcccgacaa agcgatcgga
      481 tatgcagcgg ctttttcaga cagccctact ttctccagca tttcaagtgc gatccttctg
      541 gcttcttcct tgcttttttt caaaacaatg accggaccgc tcataacatt atccagaatc
      601 gtcatattca ggaacagatt aaaatcctga aataccatgc cgatcttttt tctcagctca
      661 ttgatctcgg atgaatgctt ttcaattggc ttgtccatat ataaaatatc cccggaagtt
      721 ttattttcca gaaaatttaa gcaccgcaaa aacgtactct ttccggaacc ggaagaaccg
      781 atcaggctca cgacttcccc tttttcaatt gaaaaatcga ttcctttgag tacttccaaa
      841 tgtccaaaat atttctccag ctttttcgtt tccatgatct tcatcgttca ttcaccttca
      901 atctattttc taatttcttc aggaagaaac ttaacacata agtcatcagt aaatagaaga
      961 gcgccgcatc aaaataaata gggaaaatgg aataggtcgc tgctgctaac tgccgtgcct
     1021 tcatcattgt ctccgttatt gtaatgacac ttgccaggga cgaatccaaa acagaaacta
     1081 aaaactggtt tccaagtgcc ggaacagcat gtttaaatgc ctgagggaat atcacatgtc
     1141 taaatgcctg cagctttgtc atgccgatcg ctctggatgc ttcattctgc ccatgcggaa
     1201 ctgctaaaat agcacctctg aatatttcac ttatataagc accgttatga aatgccagtc
     1261 ccagaacagc agaggcaaaa ctgctgaaat aaatgccgat ctgcggcaga ccgtaaaata
     1321 caaaaaataa ctgtaaaagc aggggcgttc ctctaatgac cgctatgtag aactttgcta
     1381 tccatacaag cggtttgatt tttgtcattg tcatcaaatt tactataatt ccgatcacca
     1441 caccgaatat aatggaaaga atggataaga gcaacgtatt ccctattgcg ccgcggaatt
     1501 taggcatatt ttcaacggca attacaaaga aattcataat tccttcaaac atataaatca
     1561 cctttctggt cgttttgtct tttcattttt catgggctcc cataaacgga agcccatgca
     1621 tatgctatgc agcaacaacg atctgcagcc ttgttttgtt atttttcaag aatatttgtt
     1681 ccaaaatatt tcatgctgat ctcttcatat gtcccgtttt caacgatcgt ggccaatgca
     1741 ttattcagat tttccaataa ttcggtatct tcttttctga cggcaatccc gatattttcc
     1801 gcataaagca gctcaccggt cggtaccaga tcatactctt caggtgcctg cagtccaaca
     1861 aaacggctcg ttacaagacc aactgttctt ccttcggatg ctgccataaa gttatcaata
     1921 tcacttgaaa aaggaactac ttccacatca ggatagtttt cttcaaggaa cgtctggtat
     1981 gtcgttcctg ttacaacacc tacgccggag cctgcagcat cagcgatcga ggtaaggccc
     2041 agtcctgacg gagaaaagaa ttgtgaacca tcataataat aaggatccgt aaagttgacc
     2101 tgctctaatc gctcatcagt aattgccata ctgccgatga tcgtatcaaa tctttttccg
     2161 gtcagaccac ctaatattcc gtcccagtca gtcgtaatgc cttctgcttc cacgcccatc
     2221 tcttcaccaa gcgcctgtgc gatctccacg tcaaacccga tcaggcttcc atcctcatca
     2281 atgtaattaa acgggggata tcctccggaa caggcaaatg taaatttccc ttctttttgg
     2341 atcgtctccc atgtggattt ttccgaagaa cataactact tttttagaaa gcaatgtagt
     2401 gtttgtcaca aagaaaaagt attcgt
//
LOCUS       DAOSYE010000316         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34431_length_2416_cov_2.953410, whole genome shotgun sequence.
ACCESSION   DAOSYE010000316 DAOSYE010000000
VERSION     DAOSYE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34431_length_2416_cov_2.953410"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLU43_06245"
     CDS             <1..768
                     /locus_tag="PLU43_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28047.1"
                     /translation="YLGLGCILLLAAALLLVLSQLFTALWHSKKIPVMLRSHRNLLLY
                     GAAFLIIVFFAASNELSFGSALLLKVPLPQKLLDLWGTFRASGRMIWPAVYMLMLFSI
                     VICIRKLPKRLSVILLFCCLFLQILDLKDMLYEKHQEFTSRQTYESCLQNEFWTDILT
                     ERSLQHIVFYDKENLTQEQLYSFAEYAARNQFTINDFYFSRALSYPITDVAKDFFSHP
                     DDHTLYIMTYDSYQAHYDYTLSYYTFDGFIIGLKTSR"
     gene            complement(783..1655)
                     /gene="sdaAA"
                     /locus_tag="PLU43_06250"
     CDS             complement(783..1655)
                     /gene="sdaAA"
                     /locus_tag="PLU43_06250"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO28048.1"
                     /translation="MEIRSGLDLLKECTDKKQPISEVMLEREIGHGKLNRQEVLERLQ
                     NSLAIMRAASKRALAEEIHTNGGLISGNARMLASYTKEHEDLCGKAMSRAICYAMSVQ
                     EVNASMGLIVAAPTAGSAGVLPGVLFGLSDVFSYTEEQLVKGLLNAGAIGYLVMRNAS
                     VSGAEAGCQAEVGSASAMAASAVVELMGGTPKMCLNAAAFAFSNLLGMVCDPVGGLVE
                     TPCQSRNVIGVSNAFVCAQLSLSGVEHVVPFDEMTEAMLKVGKSMPESLRETAHGGCA
                     ATKAGSAACEACME"
     gene            complement(1671..2342)
                     /gene="sdaAB"
                     /locus_tag="PLU43_06255"
     CDS             complement(1671..2342)
                     /gene="sdaAB"
                     /locus_tag="PLU43_06255"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOO28049.1"
                     /translation="MEFISVFDVIGPNMIGPSSSHTAGAASIALLAKKLFPYPLKKAE
                     FILYGSFAKTCLGHGTDKALLGGILGFRADDERIRESFKIAGEQGLAYSFRTDTVKKM
                     EHPNTADIMLESTDNRQLSMRGQSVGGGKIRIVRINGIDVDFTGEYSTILVKQDDVPG
                     IIAGLSSTLSKHQVNIAYMRMYREAKGELAYTVIEADEQIPNEIIEEMSRHEHIHSVM
                     LLQET"
BASE COUNT          547 a          698 c          480 g          691 t
ORIGIN      
        1 tatctcggac tcggctgtat cctcctgctc gctgccgccc tgcttcttgt tctgtcacag
       61 ctgtttacag ctctttggca ttcaaaaaaa atacctgtaa tgctgcgttc gcacagaaac
      121 ctgcttttat acggtgccgc ctttttgata atcgtttttt tcgccgcttc caatgaactc
      181 tcttttggtt ccgctctcct attgaaagtc cctctgcctc aaaagctgct cgacctgtgg
      241 ggaaccttcc gggcaagcgg cagaatgatc tggccggctg tctatatgct gatgcttttt
      301 tccatcgtga tctgtatcag aaaactgccg aagcgcctgt ccgttatttt actgttctgc
      361 tgtctctttt tacagatcct tgatctgaag gatatgcttt atgaaaagca tcaggaattc
      421 acatcccggc agacttatga gagctgtctt cagaatgagt tctggacaga tattcttaca
      481 gaacggtcgc tgcagcatat tgtattttat gataaggaga acttaacaca agagcagcta
      541 tattcctttg cggaatacgc tgcccgtaat caatttacta ttaatgattt ttatttttca
      601 cgcgcccttt cctatccgat caccgatgtg gcaaaagatt ttttctcaca ccccgatgat
      661 catacgctgt atattatgac gtatgattcc tatcaggcgc attatgacta tacgctttct
      721 tattatacat ttgacggttt cattattggt ctgaagactt cccgctgacc ggaaccaata
      781 tcctattcca tgcacgcttc acatgccgca cttccggctt ttgttgccgc gcagccgccg
      841 tgtgccgtct cacgcagaga ctccggcatg ctctttccga ccttcagcat tgcctctgtc
      901 atttcgtcaa acggaacgac atgttctaca ccgcttaaag aaagctgtgc gcagacgaat
      961 gcattggata caccgatcac atttctgctc tgacatggtg tctcgacaag gcctccgacc
     1021 ggatcacaca ccattccaag cagatttgaa aacgcaaacg ccgccgcatt cagacacatt
     1081 ttgggagtgc cgcccatcag ctccacgact gcggacgcag ccattgccga cgcactgccg
     1141 acttctgcct gacagccggc ctctgctccc gatacggaag cattccgcat gaccagatat
     1201 ccgatcgcac ctgcattcag caggcctttt accagctgct cctccgtata ggaaaacaca
     1261 tcggaaagtc cgaacaaaac gcctggaaga acacctgccg atcctgctgt cggcgccgcc
     1321 acgatcaaac ccatggaagc attgacttcc tgtacggaca tcgcataaca gatcgcccgg
     1381 ctcattgcct ttccgcacag atcctcatgt tcctttgtat aagacgcaag catcctggca
     1441 tttccgctga tcagaccgcc gtttgtatgg atctcctctg caagcgctct tttagaagcc
     1501 gcacgcatga tcgcaaggct gttctgcaat ctttccagta cttcctgccg attcagcttt
     1561 ccgtgcccga tctccctctc taacatcact tcagaaatcg gctgcttttt atccgtacac
     1621 tcttttaaca ggtcaagtcc tgaccgaatt tccatagtat tcctccgtct ttacgtttcc
     1681 tgcagcagca tcacactgtg aatatgctca tgcctgctca tttcttcaat gatctcattc
     1741 ggtatctgtt cgtctgcctc aataaccgta taggccagct ctccttttgc ttcccggtac
     1801 atacgcatat aggcaatatt gacctgatgt ttgcttagcg ttgaagacag ccctgcaatg
     1861 atacccggaa catcgtcctg cttgacaagg atcgtgctgt attctcctgt aaaatccaca
     1921 tcgattccgt tgatccgcac gatccgtatt ttcccgcctc cgaccgactg tccgcgcatg
     1981 gaaagctgtc tgttgtccgt actttccagc ataatgtccg ccgtattcgg atgttccatt
     2041 ttttttaccg tatcggtacg aaaggaatag gcaagcccct gttccccggc aatcttaaac
     2101 gactccctga tccgttcgtc gtctgcccga aacccaagta tcccgccaag caatgccttg
     2161 tccgtaccgt gtccaagaca ggtttttgca aatgacccgt ataaaataaa ctccgctttt
     2221 tttaaaggat acggaaacag cttttttgca agcagtgcta tagacgccgc acccgccgta
     2281 tgagaacttg acggtccgat catatttggt ccgatcacat caaagacact gataaattcc
     2341 atatctgcct ccgttcctgt tcatatcttt cagtccacct tcggaagttc gtcaaagccc
     2401 ggctgcagat ccgcat
//
LOCUS       DAOSYE010000317         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34485_length_2414_cov_3.197117, whole genome shotgun sequence.
ACCESSION   DAOSYE010000317 DAOSYE010000000
VERSION     DAOSYE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34485_length_2414_cov_3.197117"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(467..1915)
                     /gene="gtfA"
                     /locus_tag="PLU43_06260"
     CDS             complement(467..1915)
                     /gene="gtfA"
                     /locus_tag="PLU43_06260"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HOO28050.1"
                     /translation="MRKLENKIMLITYADSLGCNLKELESILEKYFSRAIGGLHILPF
                     FPSSADRGFAPVTYREVEPAFGSWKDIESLADQYYMMCDYMINHISAQSEYYQDFLEK
                     KDDSVYKDLFIRYKDFWQNGEPTQEEVDAIYKRKPRAPYVEAHFADGSVEKVWCTFDE
                     EQIDINCKSDIAKKFIRDNLECLCGHGMALIRLDAFAYATKRAGTSCFFIEPDVWEFL
                     QDCEKITDEYGVMILPEIHEHYTMQQKIAAQDYYVYDFALPMLLINATYYGSTEYLKH
                     WLSICPRKQFTTLDTHDGIGVVDVKDLLPSSEIERTKEDIFKYGANVKKVYNTAAYNN
                     LDIYQVNCTYYSALGDDDNAYLLSRAVQFFAPGIPQVYYVGLLAGKNDLQFLEETKVG
                     RNINRHYYTQEEIEKEVERPVVEKLLRLMEFRNTHAAFDGEFILEDCKENEIRIRRQS
                     GENWAFLHGDFRTKMFEIRYSGADGAEQVYEV"
     gene            complement(1979..>2414)
                     /locus_tag="PLU43_06265"
     CDS             complement(1979..>2414)
                     /locus_tag="PLU43_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28051.1"
                     /translation="RGLMGLPRELTLLQKDGEYVLAAAPVKEFYQARKKVQLPFFSSA
                     KAAVELVFQMEPDGNASVSVYGMNYEYHAGTGILKIGENETELIKNLTELHLLIDKEL
                     VEISAVEDTFFAAVETKNKNTNGSVKITAHNKGACVTAYMIG"
BASE COUNT          668 a          610 c          396 g          740 t
ORIGIN      
        1 tatcagtccc agacatatga agaactgttc tttcacctgc gcctgctatt tcatacatta
       61 tttagctgta ttctgtcgga aacagaactt acggaagacc gcacaaatat attgagtcat
      121 attgaccatt atatttctac acattgtttt gaacagatca acacaaaaag catcgctgac
      181 aaatttggat tcacacctgc ctatttgagc agaatctttc aggaatacaa gcacagcagt
      241 cccatggaat acattcttcg gctacgtata gaaaaagcaa tggagctgtt ccaatgcaat
      301 ccatcccttc taatcaaaga cgtcgccgcg tacatcgggt atacagactc cctatatttt
      361 agtaaagtat ttaaaaaata cacgggccag tcaccaaaac aatatattga aagcatctct
      421 cattctacag tatcaaacat gtcaggaaat tcctgacatg ttttctctag acctcatata
      481 cctgttccgc tccgtcagct cccgaatacc ggatctcaaa cattttcgtt ctgaagtccc
      541 catgaagaaa cgcccagttt tcaccgcttt gacggcggat cctgatctca ttttctttgc
      601 agtcttccag tatgaactct ccgtcaaaag cagcatgtgt attccgaaat tccatcagac
      661 gcagcaattt ttctacgacc gggcgttcca cttccttttc aatctcttcc tgcgtataat
      721 aatgacggtt gatattgcgc cctacctttg tttcttccaa aaactgcaga tcattttttc
      781 ctgccagaag tccgacataa taaacctgcg gtatccccgg ggcaaaaaac tgcactgcac
      841 gcgaaagcag atatgcgttg tcgtcatcgc caagagccga atagtaagta cagtttacct
      901 gataaatatc aagattgtta tatgccgccg tattatatac tttctttaca tttgcaccat
      961 atttaaaaat atcttctttc gttctttcta tctcagaact tggcaaaagg tctttgacat
     1021 cgaccacacc gatcccgtca tgcgtatcca gtgttgtaaa ctgtttccgc ggacagatcg
     1081 acagccagtg ctttaaatat tctgtagaac cgtaataagt cgcattgatc agcagcatcg
     1141 gcagcgcaaa atcataaacg taataatcct gagccgcaat tttttgctgc atcgtataat
     1201 gctcatggat ctccggcagt atcatgaccc cgtactcatc cgttatcttt tcgcaatcct
     1261 gtaaaaattc ccagacatcc ggttcaataa aaaagcagct ggttcccgca cgtttcgtcg
     1321 catatgcaaa tgcatccaga cgtatcaatg ccatcccatg cccgcacaga cattccagat
     1381 tgtccctgat aaattttttc gcaatatctg atttgcagtt gatatcgatc tgctcttcat
     1441 caaaggtaca ccatactttt tcaacggagc catctgcaaa atgagcttcc acatatggag
     1501 ctcttggttt tcttttataa atggcatcca cttcctcctg cgtcggctcc ccgttctgcc
     1561 aaaaatcttt ataccgtata aagagatctt tgtatacgga atcatccttc ttttctaaaa
     1621 aatcctgata atactcactc tgtgcggaaa tatgattgat catataatcg cacatcatat
     1681 aatattgatc cgcaaggctc tcgatatcct tccagcttcc aaacgccggc tcgacctccc
     1741 gataagtcac aggtgcaaag ccccgatccg cagaagaagg aaaaaatggc agaatatgaa
     1801 gtcctccaat cgccctgcta aaatattttt caagaatact ttccagttct ttcagattac
     1861 atccaagaga atcagcatat gtaatcagca taatcttatt ttccagtttt ctcatttaca
     1921 gttatactcc tttcccatct ctccattaat ttatcacttt ctttcctttc atgaatttct
     1981 atccgatcat ataggccgtt acacaagccc ctttattatg cgctgttatt ttcacgctgc
     2041 cgtttgtatt tttatttttt gtttccaccg cagcaaaaaa agtatcttcc acggctgaaa
     2101 tttcaaccag ttccttatcg atcagcagat gaagctctgt cagatttttt ataagctctg
     2161 tttcgttttc accgatcttc agtattccgg taccggcatg atattcatag ttcattccat
     2221 acacggacac cgaagcattt ccatcaggtt ccatttgaaa aacgagctcg accgcagcct
     2281 ttgctgaaga aaaaaacggc agttgtactt tttttcttgc ctggtaaaat tcctttaccg
     2341 gtgcagccgc aagtacgtac tctccgtctt tttgcagcag ggtaagttct cttggcagac
     2401 ccatcagtcc tctg
//
LOCUS       DAOSYE010000318         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34512_length_2413_cov_3.516964, whole genome shotgun sequence.
ACCESSION   DAOSYE010000318 DAOSYE010000000
VERSION     DAOSYE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34512_length_2413_cov_3.516964"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PLU43_06270"
     CDS             <1..1446
                     /locus_tag="PLU43_06270"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component I subunit alpha"
                     /protein_id="HOO28052.1"
                     /translation="CCYAGCKGVVVGPMKDVVTITHGPIGCAFYSWGTRRNKAKPEEG
                     GQNYVPYSFCTDMRPTDIVFGGEKKLEQGIDEVMEMFHPKCIFICSTCPIGLIGDDVQ
                     AVARRAEAKYGITAIGFSCEGYKGVSQSSGHHIANNGLMKQIIGIGDKKPAGKYSVNI
                     LGEYNIGGDGWEEERILKKIGYEVVTVFTGNGSFETMQNAHLADLNLVMCHRSINYVA
                     QMMKTKYGIDWLKINFIGVGATCKSLRMIAEYFGDQELIARTEEVIAEELDAIADDME
                     YYKSRLKGKTAGIFAGGSRSHHYQVLLRDLGIETIIAGYEFAHRDDYEGREVIPTIKP
                     DADSKNIEEITVTQDEELFHERYSEEKVEELKEQGVEIDDYGGMFEDMKKGYMVVDDY
                     NMFETDELIKSMKPDIFFSGIKDKYSIQKQGVVSRQMHSYDYSGPYAGFRGAVIFGHD
                     VTMSIYTNAWAFLTPPWRTAPLLGGRIGGEN"
     gene            1446..1769
                     /locus_tag="PLU43_06275"
                     /pseudo
     CDS             1446..1769
                     /locus_tag="PLU43_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
     gene            complement(1765..>2413)
                     /locus_tag="PLU43_06280"
     CDS             complement(1765..>2413)
                     /locus_tag="PLU43_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO28053.1"
                     /translation="LIVSYSLKYAAYQKLIRERQIERAKKFVETPSKLNKKKANDPKR
                     FIEQEKCTDNGEIANHFFTTLNQEQIDKEAVYDGFYAVCTNLEDEPSSIIQINQKRWE
                     IEECFRIMKTEFKARPVYLSRKDRITAHFMTCFTALIIYRILEKKLHEKFTCDEIIAT
                     LRDMNMLILPGEGYVPTYTRTALTDALHDAFGFRTDYQITSQKNMRKILNQTKKK"
BASE COUNT          709 a          441 c          585 g          678 t
ORIGIN      
        1 tgttgttacg caggctgtaa aggcgttgtg gttggaccta tgaaggacgt ggtaacgatt
       61 acgcatggac cgatcggatg tgcgttttac agctggggaa caagaagaaa caaggcaaag
      121 ccggaagagg gcggacaaaa ttatgtacca tattcttttt gtacggatat gcgcccaaca
      181 gatatcgtat tcggcggtga aaagaagctg gaacagggta tcgatgaggt tatggaaatg
      241 tttcacccga aatgtatttt tatctgttcg acctgtccga tcggtctgat cggtgatgat
      301 gtacaggcag tggcgagaag agcagaagca aaatatggga tcacagcgat cggtttttcc
      361 tgtgaaggct ataaaggcgt atcacagtct tcgggccacc atattgcaaa taacggactg
      421 atgaaacaga tcattggtat cggagataag aaaccggccg gaaaatattc cgtcaatatt
      481 cttggtgaat ataatatcgg aggagacggc tgggaagagg aacggatcct taaaaagatc
      541 ggctatgaag tagtgaccgt atttacaggc aacggcagtt ttgaaacgat gcagaatgcg
      601 catttggcag atcttaatct ggtcatgtgc caccgctcga tcaattatgt ggcgcagatg
      661 atgaagacaa agtacggtat tgactggctg aagatcaatt ttattggagt cggggccaca
      721 tgcaagtcac tgcggatgat cgcggagtat ttcggtgatc aggaactgat tgcaagaaca
      781 gaggaagtca ttgcggaaga actggatgca attgcggatg atatggaata ctacaagtcc
      841 agactgaaag gaaaaacagc aggtattttt gcaggcggct cccgctccca tcattatcag
      901 gtattgctta gagatcttgg gatcgaaacg atcattgccg gctatgaatt tgcccacagg
      961 gatgattatg agggacgcga ggttattcct acgatcaagc cggatgcaga cagtaaaaat
     1021 attgaagaga tcacagtgac acaggatgag gagcttttcc atgagcggta ttcggaggaa
     1081 aaagtggagg aactgaagga acagggcgtt gaaatagacg attacggcgg catgtttgaa
     1141 gatatgaaga agggctatat ggtcgtggat gattataata tgttcgaaac cgatgagttg
     1201 atcaaatcca tgaagccgga catttttttc tcgggtatca aagataagta ttcgatccaa
     1261 aaacagggag tggtttcaag acagatgcat tcctatgatt attcgggacc atacgcggga
     1321 ttccggggag cagtcatatt cgggcatgat gtcacgatga gtatttatac caatgcatgg
     1381 gcatttctta ctccgccatg gaggacagct ccgctgcttg gaggaagaat cggaggtgaa
     1441 aattaatgct ggatttaacg ccaaaggaaa taatggaaaa aagacatatt acgatcaatc
     1501 catgtaagac atgcgaaccg gtcggcgcta tgtatgccgc actcggcgtg gaaaactgta
     1561 tgccgcacag ccacggttcc caggggtgct gctcttatca cagaacagtg ctgtcccgtc
     1621 attttaagga gccggcaatg gccacctcaa gctcctttac agagggtgcg tcggttttcg
     1681 gcggaagaag caacctgacg acttccgtaa aaaatatttt tgatatttat gatccggaca
     1741 tcattgcagt acatacgacc tgtcttactt ctttttcgtt tgattcaaga tttttctcat
     1801 atttttttga gatgtaatct gataatcagt tctaaaacca aatgcatcat gtaatgcatc
     1861 agtcagagcg gttcttgtgt atgtaggaac ataaccttca ccgggtaaga tcaacatatt
     1921 catgtctctt aaagttgcaa tgatttcatc gcatgtaaac ttctcatgta gtttcttctc
     1981 taaaatccta taaataatga gtgcagtaaa acaggtcatg aaatgagcag ttattctgtc
     2041 ttttcttgat aaatacacag gtcttgcttt gaattctgtt ttcataattc gaaaacattc
     2101 ttctatttcc caacgtttct gattgatttg aataatagat gatggttcat cctcgagatt
     2161 tgtacataca gcataaaacc catcatatac agcttcttta tcaatctgtt cctgatttag
     2221 agtggtaaaa aaatggtttg ctatttcacc attatcagta catttttcct gctcaataaa
     2281 tcgtttaggg tcatttgctt ttttcttatt cagtttagaa ggggtttcaa cgaacttctt
     2341 tgcccgttca atctgacgtt ctcttattaa tttctgatat gcagcatatt taagagaata
     2401 ggatacgata aga
//
LOCUS       DAOSYE010000319         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34576_length_2410_cov_2.680679, whole genome shotgun sequence.
ACCESSION   DAOSYE010000319 DAOSYE010000000
VERSION     DAOSYE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34576_length_2410_cov_2.680679"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLU43_06285"
     CDS             <1..908
                     /locus_tag="PLU43_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO28054.1"
                     /translation="IRETLFFHTAGRTAADIIWKIRLPRIAAVVVLGGALALSGFLLQ
                     TFFHNPIAGPYVLGISSGAKLMVALTLIYFLGRMKTPGSFVLILAAFFGAMLSMGFVL
                     FMAGYVRNIAMLIICGIMIGYIASSITDFVITFAAQEDIVNLHNWSLGSFSGMSWQNV
                     GMMSLVVFMTLAAVFFMAKPMGAYQLGEEYAKSMGVNIKAFRVGLILISSLLSACVTA
                     FAGPISFVGIAVPHLMKFFLKTARPMLLIPACFLGGSVFCMGCDLLARTLFAPTELNI
                     SSVTAVFGAPVVIVLLIKRGRSVHE"
     gene            901..1656
                     /locus_tag="PLU43_06290"
     CDS             901..1656
                     /locus_tag="PLU43_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28055.1"
                     /translation="MNDAVVLHTADLCVGYKNMPVLSELNLKAERGKLLTLIGPNGSG
                     KSTVLKSIAKQLAPLAGTVYLEGAALETLSYSDTAKKAAVVLTKKPHAELMTCFEVAA
                     AGRYPYTGRFGMLTKKDKQIVLEAMRLVDAADLADRQFRKLSDGQKQKILLARALCQE
                     PEILILDEPTSFLDLRHKIDILNVLKETAEKKNLAVVMSLHDLDLARQYSDTVMCVRG
                     EHISHIGTAEEIFRQEIICELFELTRQEYEKYV"
     gene            1783..>2410
                     /locus_tag="PLU43_06295"
     CDS             1783..>2410
                     /locus_tag="PLU43_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HOO28056.1"
                     /translation="MLGGCGSKKAEYEKPVILVVSFGTSYNDSRDKTIGGIEQAIADA
                     NPDYEVRRAFTSQIIIDKLKERDGLEIDNVKEALDRAVADGVKTLVVQPTHLMNGYEY
                     TDLMDELSEYEDSFDQIAVGEPLLTSDDDFEAVISAITAHTESYCSDDTAIIFMGHGT
                     EADSNSVYEKMQNMISADGFSDYYIGTVEATPSLEDVIADATAGGYTKV"
BASE COUNT          645 a          480 c          671 g          614 t
ORIGIN      
        1 aaatcaggga gacgctgttt tttcatacgg caggcagaac cgcggcggat attatctgga
       61 agatccggct gccaaggatc gctgccgtag ttgttctcgg aggagcgctt gcactgtcgg
      121 gatttcttct gcagactttt ttccataatc cgatcgcagg accgtatgtg cttggtattt
      181 cttcaggggc caagctgatg gtggcactga ccctgatcta tttccttgga aggatgaaga
      241 caccaggttc ctttgtactg attctggctg cttttttcgg agcaatgctg tccatgggat
      301 ttgtactttt tatggccggc tatgtacgca atattgcaat gctgatcatc tgcggcatca
      361 tgatcggcta tattgcttcg tcgatcacgg attttgtgat cacatttgcc gcacaggagg
      421 atatcgtaaa tctgcataac tggtcacttg ggagcttttc aggcatgtca tggcagaatg
      481 taggtatgat gtcgcttgtt gtctttatga cactggcagc cgtttttttc atggcaaaac
      541 caatgggggc ttatcagctc ggggaggagt acgcaaaaag catgggggtg aatatcaaag
      601 cattccgtgt cggtctgatc ctgatctcga gcctgctgtc tgcgtgtgta acggcgtttg
      661 ccgggccgat ctcgtttgtt gggattgccg tgccgcacct gatgaaattc tttttaaaaa
      721 cagccaggcc gatgctttta attccggcat gctttttagg cggaagcgtt ttctgcatgg
      781 gctgcgatct gcttgccagg acactgtttg cgccgaccga attgaatatc agctccgtta
      841 cggccgtatt tggggcgccg gtcgtaatcg tgctgcttat aaaaaggggg cgcagtgtac
      901 atgaatgatg cggtggttct gcatacggct gatctgtgcg ttggttataa aaatatgccg
      961 gttctctcag agcttaatct taaagcagag aggggaaagc tgcttacact gatcggcccg
     1021 aacggatccg gaaaatcaac agttctgaaa agtattgcta agcagcttgc accgcttgcg
     1081 ggtacggttt atctggaagg agcggcattg gaaacgctgt cctattccga tacggcaaaa
     1141 aaagcagcgg tcgtattgac aaaaaagccc catgcggaac tgatgacctg ttttgaagtg
     1201 gcggcagcag gcagatatcc gtataccgga cggttcggaa tgctgacgaa aaaagacaaa
     1261 cagatcgttt tagaagccat gcggctcgta gatgcggccg atcttgcaga caggcagttt
     1321 cggaagctga gtgacggaca gaaacagaag atactgcttg cgcgggcgct gtgtcaggag
     1381 ccggaaatac taatactgga cgaaccaacg tcgtttcttg atttaagaca caagatcgat
     1441 attttgaacg tattgaaaga aaccgcagaa aaaaagaatt tggcggttgt gatgtcactg
     1501 catgatctgg atcttgcaag acagtattcg gatacggtca tgtgcgtcag aggagaacat
     1561 atttcacata tcggaacagc agaagaaatt ttcagacagg agatcatttg tgagctgttt
     1621 gagctgaccc ggcaggaata cgagaaatat gtataaagaa gaaacttaat attgcatatg
     1681 atatatggcg ggatatccgc aaatcaatga tttcagggag gatgaaatat gaaagcaaca
     1741 aaaaagacag tagtatgtgc ggcggcaata ttgcttgcag gcatgctcgg cggctgtgga
     1801 agcaagaaag cggaatatga gaaaccggtg attcttgtcg tcagcttcgg aacaagctat
     1861 aacgacagca gggataagac gatcggcggg atcgaacagg cgatcgccga tgcaaatccg
     1921 gattatgagg tacgccgggc ttttacgagc cagatcatta ttgacaagct gaaagaacga
     1981 gatggtctgg agatcgacaa tgtaaaagag gccttggaca gagcggtggc agacggcgta
     2041 aaaacgcttg tcgtacagcc gacccatttg atgaacgggt atgagtatac ggatctgatg
     2101 gatgaattaa gtgaatatga agattctttt gaccagatcg cagtaggaga gccgcttttg
     2161 acgagcgatg atgattttga agctgttatt tctgcaatta cggcacatac ggaaagttac
     2221 tgcagtgacg atacggcgat catttttatg ggacacggta cggaggcaga ttccaacagt
     2281 gtttatgaaa aaatgcagaa tatgatcagc gcagacggtt tttccgatta ttatatcgga
     2341 acagtagagg cgaccccttc cttagaagac gtgattgccg acgcgacagc cgggggatat
     2401 acaaaagtag
//
LOCUS       DAOSYE010000320         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34591_length_2409_cov_3.158454, whole genome shotgun sequence.
ACCESSION   DAOSYE010000320 DAOSYE010000000
VERSION     DAOSYE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34591_length_2409_cov_3.158454"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="PLU43_06300"
     CDS             complement(<1..1354)
                     /locus_tag="PLU43_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28057.1"
                     /translation="MILELVRKGTKIRNFFIFMILVLGIAGLIYMNRTGKEYRMFNQV
                     DVLTEGNDIETQRLSLPKGTYYMVVNYVVSEDIPVTVYIDQEHQIEDTLVKNTENGYY
                     TLSFEVEKSTSLFHLIFADGAIHDFQMYNYEMTADRVLYNDDTYYAIVFILLALSIYI
                     LLQCKVVWEMSAKRKTIILTLVALTLFSSYPMFTNYLVHGHDLLVHLMRIEGVKDALA
                     DGQFPAMIYPDSNNGYGTLGFTYPTLFLYFPALLRMCNVSMVTAYQSLIIMINAATAA
                     AIFLSVKSVSDSDYASLIASVLYMLAPYRLSDLYVRSALGESLAMIFLPITLAGLFHI
                     FLGEKKKWYLLVIGYSGILQSHMLSCLLIAFVSLIVGLVLVRHLFRERRIFSLLKAIG
                     LFLGVNLWYMIPFWAYSQNPLGMRLIENPDFYEDAVFLGQLFMTKSSTYVPLGTAEGI
                     G"
     gene            complement(1686..>2409)
                     /locus_tag="PLU43_06305"
     CDS             complement(1686..>2409)
                     /locus_tag="PLU43_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO28058.1"
                     /translation="VTGSGQEQKYGNPHDYQADKVLVLYLPDCHESLAGIVAGRVKER
                     YYKPVIVLTKGEDGIKGSGRSIEAYSMYEELMKCRDLFTKFGGHKMAAGLSMHESAIS
                     ELRERLNENAVLSEEDLTEKVTIDIPMPVMYATETFIEELNLLAPFGIGNPRPLFAQK
                     DLKIRSLQVLGKNRNVVRLKLETLQEAGRAPVITDAICFGEGDTIEAELSKKESVSIV
                     YYPEFNEYLGRRNMQIVIRDYQ"
BASE COUNT          710 a          597 c          418 g          684 t
ORIGIN      
        1 cgccaatgcc ttctgctgtg ccaagcggta catatgttga tgactttgtc ataaaaagct
       61 gtcctaaaaa tacggcatcc tcataaaaat ccggattctc gatcagtctc attccaagcg
      121 gattttggga atatgcccaa aatggtatca tataccataa attaactccc agaaacagac
      181 cgatagcttt taacagggaa aagatccttc tttccctgaa caaatgccga accaatacaa
      241 gaccaacgat cagcgaaaca aatgcgatca gcaggcagct tagcatatgg gactgtaaaa
      301 tacccgaata tccgatcaca agcaaatacc atttcttttt ttctccaaga aaaatatgaa
      361 acagccccgc cagcgtaatc ggcaggaaga tcatcgcaag agactctcca agtgcacttc
      421 tgacatacag gtcactcaga cggtaaggcg ccagcatata cagtacgctt gcaatcagcg
      481 atgcatagtc tgaatcagat acggatttca ccgataaaaa aatcgcagcg gcggttgccg
      541 cattgatcat aatgatcagc gactggtaag ccgttaccat tgaaacattg cacatgcgca
      601 gcagcgccgg aaaatacaga aaaagggtcg gatacgtaaa gccaagcgta ccgtatccgt
      661 tattgctgtc cggatagatc atcgctggaa actgaccgtc agccaaggcg tcctttaccc
      721 cttcaatacg catcagatga acaagcaggt cgtggccatg cacgagataa ttcgtaaaca
      781 tcggatagct tgaaaacagc gtaagcgcca ctaatgtcag aataatcgtt ttacgttttg
      841 ccgacatctc ccatacaact ttgcactgca aaagtatata aatagagagc gcaagcagaa
      901 taaatacgat cgcgtaataa gtatcgtcat tatacaagac cctgtcggct gtcatttcat
      961 aattgtacat ctgaaaatca tgaattgcac catctgcaaa tatcaggtga aacagacttg
     1021 tacttttttc cacttcaaaa gaaagtgtat aataaccatt ctccgtattt ttaacaagcg
     1081 tatcttctat ctgatgttcc tggtcaatat aaaccgtaac gggaatatct tcggagacaa
     1141 catagttcac caccatgtaa tacgtgcctt ttggaagcga cagtctctgg gtctcaatat
     1201 catttccttc cgtgagcaca tcaacctgat taaacatccg gtactctttg cctgtgcggt
     1261 tcatgtagat cagaccggca attccaagta ccagaatcat aaagataaaa aaatttctta
     1321 tctttgtacc ttttcggacc aattccaata tcatcgacta ttctccttct ataattggtc
     1381 ccccgccaca tactaattag tataccacga ttatttccag tcataaacct tttttacaat
     1441 ttttaaatgc tcgttttacc ataaatataa cgtctgtttt tttaagtttt ttataaatta
     1501 tctccttttt cttaagattc ttaaaacttt ttttatacaa taaacacaca ttcaacacat
     1561 ttaatcaata taataggtat tggatagttg aaaacactca ctatctcccc cctcataaga
     1621 gaacgtagag ccggcaccgc aattatgcgg attccggctc tctctctgtt ctatttttta
     1681 tgattctatt gataatctct gatcacgatc tgcatgtttc ttctccctaa atactcattg
     1741 aactcgggat aatatacaat ggaaactgat tccttcttag acagttcggc ttctattgtg
     1801 tccccttctc caaaacaaat ggcatctgtt ataacaggag ccctgcccgc ctcctgcaaa
     1861 gtctccagct tcagcctgac aacattgcga ttttttccaa gtacctgcag acttcttatc
     1921 tttagatctt tctgcgcaaa cagcggccgc ggatttccga taccaaaagg cgcaagcaga
     1981 ttcagttctt caataaacgt ttccgttgca tacatgaccg gcatgggaat atcaattgtt
     2041 actttttctg tcagatcctc ttcacttaga acggcatttt cattcagacg ctctcttagt
     2101 tcgctgatcg cagactcatg catggacaga ccggcagcca ttttatgacc tccgaatttc
     2161 gtaaacagat cccggcattt catcagttcc tcatacatcg aatacgcttc aatagaacgt
     2221 cccgaaccct tgataccgtc ttctcccttt gtcaatacga tgaccggttt ataatatcgt
     2281 tccttcacac gcccggccac gattccggca agactttcat gacaatccgg caggtacagg
     2341 acaaggacct tatccgcctg ataatcatgc gggttcccgt acttttgttc ctgaccgctg
     2401 ccggtcaca
//
LOCUS       DAOSYE010000321         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34891_length_2396_cov_2.871850, whole genome shotgun sequence.
ACCESSION   DAOSYE010000321 DAOSYE010000000
VERSION     DAOSYE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34891_length_2396_cov_2.871850"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..2116
                     /gene="rnr"
                     /locus_tag="PLU43_06310"
     CDS             35..2116
                     /gene="rnr"
                     /locus_tag="PLU43_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOO28059.1"
                     /translation="MKEEVYVPMKEKELAIFLQVPKENRESLREVLQQLLDEKEIQIS
                     KRGKYSIADKKTLQGVFVATQHGYGFVEVEGEEEDYFVAEKNVDNAMHGDTVEFEPIA
                     ASYKRGKRKEAVVVKVLERATNLLVGTFDKAKNRYGFVIPDNHRFNQDIFIPIEKSNG
                     ALDGHKVVVEITDYGSRSKSPEGKVVEILGHINDPGVDVLSIIKSYDLPVAFPERVMN
                     QTARISDEVSEADMAGRTDLRNVTMVTIDGEDTKDLDDAVSISMEGDCYLLGVHIADV
                     TNYVQENSALDREALGRGTSIYLADRVIPMLPHRLSNGICSLNENQDRLALSCLMRVD
                     KNGEVIDHEIVETVIRTNRRMTYTAVNAILEDDDEELKKEYEELVPQFYLMRDLAHIL
                     REKRKKRGSIDFDIPETKLILDKEGHPIEIRPYERNTATKLIEDFMVLANETIAEHFF
                     WQELPFVYRTHEKPDPEKIKKLAAFIRNFGYGIKIKNEEIHPKELQKLLARIEDTPEE
                     ALISRLTLRSMQRASYSTECTGHFGLACKYYCHFTSPIRRYPDLQIHRIIKDSIRGRL
                     KEARIEHYHKILGEVAKQSGSRERLADEAERETEKLKKAEYMEDKIGEIYTGVISGIT
                     NWGIYVELPNTIEGLVHVSKITGDFYYYDENSYELIGEASGRRYKLGQTVSVCVNGVD
                     KASRSVDFVLA"
     gene            2146..>2396
                     /locus_tag="PLU43_06315"
     CDS             2146..>2396
                     /locus_tag="PLU43_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOO28060.1"
                     /translation="MAKENNKNGFKLIANNKKAYFDYFIEDKYEAGISLYGTEIKSLR
                     MGKCSIKEAFVRIEKGEVFIYGMHISPYEKGNIFNRDPLR"
BASE COUNT          769 a          418 c          623 g          586 t
ORIGIN      
        1 cccatctcaa ttcatcaata ccaacccggt agaaatgaag gaagaagtat atgtaccgat
       61 gaaggaaaaa gaacttgcaa tttttcttca ggttccaaaa gaaaacaggg agtctttacg
      121 ggaagtgctg caacagctgc tcgatgaaaa agaaatacag atctccaagc gcggtaaata
      181 ttccattgct gataaaaaga cattgcaggg agtttttgta gcaacgcagc atgggtacgg
      241 gtttgtggag gtggaaggcg aagaagagga ttattttgtt gctgaaaaaa atgtagacaa
      301 tgccatgcat ggtgatacgg ttgagtttga gccgatagcc gccagctata aaagaggaaa
      361 aagaaaagaa gccgttgtcg ttaaagtgct ggaacgggca acaaatctgc ttgtaggcac
      421 gtttgacaaa gcaaaaaaca gatatggatt tgtaatcccg gataatcatc gtttcaatca
      481 ggatattttt attccgatcg agaaatcaaa cggcgcgctg gacggccata aggttgttgt
      541 tgagattacg gattacggca gccgatcaaa aagtccggaa gggaaagttg ttgaaattct
      601 ggggcatatc aatgaccctg gcgttgatgt tttatcaatt atcaaaagct atgatctgcc
      661 cgtggcattt ccagagcggg tcatgaatca gacagcacgg atttccgatg aagtatccga
      721 ggcggatatg gccggaagga cagatttaag aaatgtaaca atggtaacga tcgacggtga
      781 agatacaaag gatctggatg atgcggtcag tatttcaatg gaaggagact gttatctgct
      841 gggtgtacat attgcggacg ttacgaatta tgtacaggaa aacagcgcat tggacaggga
      901 agccctcgga cgtgggacaa gtatttatct tgctgacagg gtgattccaa tgctgccgca
      961 cagactcagc aacggcattt gctctttgaa cgaaaatcag gacaggcttg cactcagctg
     1021 tttgatgaga gtagataaaa acggcgaggt cattgaccat gaaatagtcg aaacagttat
     1081 ccgaacgaac cgcagaatga cttatacagc cgttaatgca atcctggagg atgacgatga
     1141 agaactgaaa aaggaatatg aggaactggt tccgcaattt tatctgatgc gtgacctggc
     1201 tcatattctg cgggaaaagc gaaaaaagcg cggttccatt gattttgaca taccggagac
     1261 aaaattgatt cttgataagg aaggacatcc gatcgagatc aggccttatg agcgcaatac
     1321 tgcgacaaag ctgatagagg atttcatggt gcttgccaat gagacgattg ccgaacattt
     1381 tttctggcag gagcttccgt tcgtatatcg gacacatgaa aaaccggatc cggaaaaaat
     1441 taagaagctg gctgctttta tccggaattt tggttatgga attaaaataa agaatgaaga
     1501 gatccatcca aaagagcttc agaagctgct tgccagaatc gaagatacgc cggaggaagc
     1561 gctgatcagc cgattgacgc tgcgttcgat gcagagagca agctattcga ccgaatgtac
     1621 cgggcatttt ggactggcct gcaagtatta ttgtcatttt acatcaccga tccggcgcta
     1681 tcctgatctg cagatccaca ggattattaa agacagtatc cgcgggagac tgaaagaagc
     1741 acggatcgag cattatcata aaatactggg agaagttgcg aagcagtccg gaagcagaga
     1801 acggcttgcg gatgaggctg aacgtgagac agaaaaactg aaaaaggcgg aatatatgga
     1861 ggataagatc ggagaaatat ataccggtgt gatttccggt attacaaact ggggaattta
     1921 tgtggagctg cctaatacga tcgaaggtct tgttcatgta tcaaaaataa cgggcgattt
     1981 ctattattac gatgaaaaca gttatgaatt gatcggtgag gcatcaggcc ggcgctataa
     2041 acttggacag acagtttccg tatgtgtcaa cggggttgat aaggcttcgc gttcggtaga
     2101 ttttgtgctt gcctgaagat tgatttaaac gggaagggaa ttgtaatggc aaaggaaaat
     2161 aataaaaatg gttttaaact aatagcaaat aataaaaagg catattttga ttattttatt
     2221 gaagacaaat atgaggcagg tatctcctta tacggtacgg aaatcaagtc gctgcgtatg
     2281 ggaaaatgca gtatcaagga ggcgttcgtc cgtattgaaa aaggcgaggt atttatttac
     2341 ggcatgcaca tcagtccgta tgaaaaagga aatattttca acagagatcc tctgcg
//
LOCUS       DAOSYE010000322         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_34985_length_2392_cov_3.620454, whole genome shotgun sequence.
ACCESSION   DAOSYE010000322 DAOSYE010000000
VERSION     DAOSYE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34985_length_2392_cov_3.620454"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLU43_06320"
     CDS             <1..1408
                     /locus_tag="PLU43_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO28061.1"
                     /translation="LQPPVAGQIVLGWDPAFRTGCKLAVVDATGKVLDTKVIYPTAPQ
                     FKVNEAKAELKRLIKKYGVTLISVGNGTASRESEMIIVELLKEIDEKVQYVIVNEAGA
                     SVYSASKLATEEFPNFDVGQRSAASIARRIQDPLAELVKIDPKSIGVGQYQHDMNQKK
                     LSDALHGVVEDSVNKVGVDLNTASASLLEYISGITKAVAKNIVDYREKNGRFTNRRQL
                     LKVGKLGPKAYEQCAGFLRILNGENPFDATSVHPESYEATKKLLDKMGLTMEDVRAVQ
                     AAAAKNKEAVKKAPAGSTKKQAVKQTGIRNQNTAMGQALAAAMGTVKPEREPVYTPES
                     TGTVGVLEKKIVDKKKMALELGIGEITLTDILKELQKPARDPRDDMQKPILRSDILEM
                     KDLTPGMILKGTVRNVIDFGAFVDIGVHQDGLVHISQITDKYIKHPLEAVAVGDIVEV
                     KVLSIDVPKKRIALTMKF"
     regulatory      1490..1666
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1779..>2392
                     /locus_tag="PLU43_06325"
     CDS             1779..>2392
                     /locus_tag="PLU43_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO28062.1"
                     /translation="MNFSELFASAKENAAFLGVSLIVIIIVAVIAGVSEKLIGCKHDE
                     RMRTKRIAFIGIFSAIAAILMYLEIPLWFAPPFYKIDFSEVPVLICAFAYGPVAGVIA
                     ELLKVVLKLFIKGTSSAFVGDLANFIIGCTMILPASIIYHIKKTRKTALLGMTAGTVI
                     MTIFGSIFNAVYLLPTFSVLYGMPLEAIIAMGTAVNVNITDITTL"
BASE COUNT          707 a          424 c          656 g          605 t
ORIGIN      
        1 gttgcagccg cctgttgcgg ggcagatcgt gcttggctgg gatcctgctt ttcgaacagg
       61 ctgtaagctt gcggtcgtag atgcaacagg aaaagtgctt gatacaaaag tgatttatcc
      121 gactgctccg caatttaaag tgaacgaggc aaaagcagag ctgaaaagac tgataaagaa
      181 atatggtgta acattgattt ctgtcggtaa cggcacagct tcccgtgaat ctgaaatgat
      241 cattgtggag ctgctgaagg agatcgatga aaaagtacag tatgtgatcg tgaatgaggc
      301 gggagcttcg gtttattccg caagtaaact tgcaacggaa gagtttccga attttgatgt
      361 aggccagagg agtgcggcat cgatcgcaag gcgtattcag gatccgcttg cggagcttgt
      421 caagatcgat ccgaagtcga tcggagtagg tcagtatcag catgacatga atcagaaaaa
      481 gctgtctgat gccctgcatg gggttgttga ggatagtgtg aataaggtcg gggttgatct
      541 gaatacggca tcggcgtctc tgcttgagta tatatccggc attacaaagg ctgttgcaaa
      601 aaatattgtg gattacaggg agaaaaacgg caggtttaca aaccggagac agcttttgaa
      661 ggtagggaag ctgggaccta aggcatacga acagtgtgcg ggttttctgc gtattttaaa
      721 tggagagaat ccttttgacg caacaagcgt tcatcctgag tcttatgagg ccacgaagaa
      781 gctgcttgat aagatgggac tgacaatgga ggatgtgcgc gcagtacagg cggctgccgc
      841 taaaaataaa gaagcagtaa aaaaagctcc ggccgggagt acaaagaaac aggcggtaaa
      901 gcagacaggg atccgcaatc agaatacggc aatgggacag gcactggctg cggcaatggg
      961 aactgtgaaa ccggaaaggg aaccggtgta tacgcccgaa agtaccggaa cagtcggtgt
     1021 gcttgaaaaa aagatcgttg ataagaaaaa aatggcattg gagcttggca ttggtgagat
     1081 cacattaacg gatattttaa aagagctgca gaaaccggcc agagaccccc gggatgatat
     1141 gcagaaaccg atcctgcgtt ccgacatatt ggagatgaag gatctgactc cgggaatgat
     1201 tttaaagggg acggtcagga atgtgatcga ttttggtgca tttgtggata ttggcgttca
     1261 tcaggacgga cttgtccata tttcgcagat caccgacaaa tatattaagc atccgcttga
     1321 agcagtagcg gtcggtgata ttgtggaagt aaaggtgctg agtattgatg taccgaaaaa
     1381 aaggatcgca ctgacaatga aattctgacg acaattttgt attgacaaag cggcagtaat
     1441 gaagtatcgt gaaaatgaac agaagctgtt ctgtttaaat tgaataagga aaattcttcg
     1501 gggtaaggtg taattcctaa ccggtggtga tgacggaatg aagatgcagc agatgcatgg
     1561 attcataaac gtacaagtcc gcgacccgtt tgtatgagaa agatgcgaac ggctgatttg
     1621 gtataatcca aaaccgacag taaagtctgg atggaagaag aaacgtgaaa cggcaaagca
     1681 taagcgtaag ctttttcgaa tgataaaccc ccaatattta tttgggggtt ttctatttaa
     1741 aacgagaatt tcccttagaa agaaaagagg aaaagaacat gaatttcagt gaattatttg
     1801 cttcggcaaa ggaaaatgca gcttttttgg gagtcagtct gatcgtgatc atcatagtgg
     1861 cagtcattgc cggtgtatct gaaaagctga tcggatgtaa gcatgatgaa cgcatgcgta
     1921 cgaagcgaat tgcgtttatc ggtatttttt cagcaattgc ggcaatcctg atgtatttgg
     1981 agattccgct ctggtttgcg ccgccgtttt ataagatcga tttcagcgaa gtaccggtgc
     2041 tgatctgtgc atttgcatac ggtccggttg caggcgtgat tgcggaactt ctaaaggtcg
     2101 tattaaagct gtttataaaa ggcacttcat ctgcgtttgt cggtgacctt gccaatttta
     2161 taatcggttg cacgatgatc ctgcctgctt cgattattta tcacattaaa aaaacaagaa
     2221 agacggcgct gcttggtatg acagcgggga cagtgatcat gactatattt ggaagtattt
     2281 ttaacgcggt atatctgctg ccgacatttt cggtcctgta tggaatgccg cttgaagcga
     2341 tcatcgcaat ggggacggct gtcaatgtaa acattacaga tatcacaacg ct
//
LOCUS       DAOSYE010000323         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35028_length_2390_cov_2.817987, whole genome shotgun sequence.
ACCESSION   DAOSYE010000323 DAOSYE010000000
VERSION     DAOSYE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35028_length_2390_cov_2.817987"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1541
                     /locus_tag="PLU43_06330"
     CDS             51..1541
                     /locus_tag="PLU43_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HOO28063.1"
                     /translation="MKKGVMRVAACCIAGALAVTCFPAVSVSAASSSSVLPAAGGALA
                     TGEGISISDIKASIARSAAKQTAVATHSTITGSVSGANLQIGAGSVVGITAEKAETPA
                     SKAVQESATVNGTSVVGSASDAAAISVATDTEGDGVSADAVTDTEDDTVSDNTTDSDT
                     TVSENEVSTNAVPSLEIKPEAADVAEGEEDLSGTIIAQVSNYVNVRDVPSEDGEVVGK
                     LYNNSAGEWLGKEGDWYKIKSGSVVGYVKGEYVVTGQAAIELAAEVGNRIAVVNTTTL
                     KVREEPSADAAVLGLVPNEDRLTVLDETDEWIKVSIEEGDGYVSKEFVNVSTEFVQAE
                     STAEEEARLQKEEDERKKANAAAAAAQKKNSSSSSSSSSSSGYSVSGSSTGAAVANYA
                     LQFVGNPYVYGGTSLTNGTDCSGFTMSVYKNFGVSLPHSSGAQRSVGYDVGSIANAQP
                     GDLVCYSGHVALYIGNGQIVHASTAKTGIKISNASYRQILAIRRIF"
     gene            2015..>2390
                     /gene="raiA"
                     /locus_tag="PLU43_06335"
     CDS             2015..>2390
                     /gene="raiA"
                     /locus_tag="PLU43_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOO28064.1"
                     /translation="MKFIIVGKNIDLTEGLRSAVEDKIGKLERYFTPDTEVHVTLSVE
                     KERQKIEVTIPVKGNIIRSEQVSNDMYVSIDLVEEIIERQLKKYKNKLVDSKHGLGNF
                     RQEFIEKEYHDDDEIRIVRSKRF"
BASE COUNT          770 a          408 c          633 g          579 t
ORIGIN      
        1 ttgtaataat taaatgattt ttaggggata aatattcgga ggtccatttc atgaagaaag
       61 gtgtaatgag agttgcggca tgctgtatag caggcgcgct ggcggtgact tgtttcccgg
      121 cggtgagtgt aagtgctgct tcatcttctt cggttttgcc ggcagcaggg ggagcgctgg
      181 caacaggcga aggcatttcc ataagtgata taaaggcgtc gatcgcaagg agtgctgcaa
      241 agcagacggc ggtagcgacg cattctacaa taacaggcag tgtcagcgga gccaatctgc
      301 agatcggagc gggttcggta gttggtataa ctgcagaaaa ggcggaaacc ccggcatcca
      361 aagcggtcca ggaatcagct acggtaaatg gtacatccgt agtggggagt gcatcggatg
      421 cagctgcgat atcggtagct accgatactg aaggagatgg tgtatcggca gatgccgtca
      481 ccgatacgga agacgatacg gtttcggata atacaacgga ttccgataca acggtctcgg
      541 aaaatgaagt cagcacgaat gcagtgccgt cacttgaaat aaagcctgag gcagcggatg
      601 tggccgaagg tgaggaagac ttatcaggaa cgatcattgc gcaggtaagt aattatgtca
      661 atgtcaggga tgttccaagc gaagacggcg aggtagtagg taagctttat aataattcgg
      721 ccggtgagtg gcttggcaaa gaaggcgact ggtataagat caaatccggt tccgtagtcg
      781 gttatgtaaa aggtgaatat gtagtgaccg gacaggcagc gatcgaactg gctgcagaag
      841 tagggaacag gattgccgta gtaaatacga cgacattaaa agtcagggaa gagccgagcg
      901 cagatgctgc cgtactgggt cttgtaccaa atgaggacag gctgacggtg cttgacgaaa
      961 ccgatgagtg gataaaggta tccatcgagg aaggtgacgg atacgtttcc aaggaatttg
     1021 taaatgtttc gactgaattt gtacaggctg aatcaacagc agaagaagaa gcaagacttc
     1081 agaaggaaga agacgagcga aagaaagcca atgcagccgc agcggcagca cagaaaaaga
     1141 acagttccag ttcttcaagc tcctcctcca gttcaggata ttccgtatcc ggaagcagta
     1201 ccggtgctgc ggtagcaaat tatgcattgc agttcgttgg aaatccatat gtatatggtg
     1261 gtacgagcct gacaaacgga acagattgtt caggatttac aatgagtgtt tataagaact
     1321 ttggtgtttc cctgccgcat tcttcaggtg cacaaagaag cgtagggtat gatgtaggaa
     1381 gtattgcaaa tgctcagcct ggtgatcttg tatgttattc cggtcatgta gcgctttata
     1441 tcggaaatgg acagatcgta catgcgagta cggctaaaac cggaattaag atttccaatg
     1501 caagttatcg tcagattctg gcgatcagac gaatcttcta aaatgataca atataggata
     1561 ctgtaaaata ataacgcttc gaacatctct gttcggagcg ttatttattt atgaaacagc
     1621 cggataaaaa agatgtaatt tttgcttgac aaatcttttg ttagacatca tgcacaaatt
     1681 gtaaaaaaat catgaaggac agcagtaaaa tattgcagaa tgccataaaa cttatccaca
     1741 aaattcaaag tcaaagaaag cgtaaaatga acttatgcac gaagttgtac acattatcca
     1801 caggaagatc agaaaatacc atttaaaaaa atggaacaaa cgttttgtta aaaaataata
     1861 aaaaacagat gatgtttatt ttaagagatg cattgtaatt gcaaacagca agtaaaatgt
     1921 gatatactgt attcacataa taaatacgcg gcaggtatgg atattactgc ctgaatagcc
     1981 ggataagcgc gtatcagtga aaggagtggc tggcatgaaa tttattattg tcggtaagaa
     2041 tattgattta acggaagggc ttaggagcgc agtagaggac aagattggaa agctggagcg
     2101 ttatttcacc cctgataccg aagttcatgt gacgttgagc gtggaaaagg aaaggcagaa
     2161 gatcgaagta acgatccctg taaaaggtaa tattatacgt tcggagcagg tcagcaacga
     2221 catgtatgtt tccattgatc ttgttgagga aattattgaa agacagctta agaaatataa
     2281 aaataagctt gtggacagta agcatggact cggcaatttc agacaggaat ttattgaaaa
     2341 agagtatcat gatgatgatg agatcaggat cgtgcgttcc aaaaggtttg
//
LOCUS       DAOSYE010000324         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35152_length_2385_cov_3.348069, whole genome shotgun sequence.
ACCESSION   DAOSYE010000324 DAOSYE010000000
VERSION     DAOSYE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35152_length_2385_cov_3.348069"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..906
                     /locus_tag="PLU43_06340"
     CDS             7..906
                     /locus_tag="PLU43_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28065.1"
                     /translation="MMDRAKRGHLSLSKKQNLTGWIFLAPAAILIAAMSFYPMIKALL
                     LSFKTGIGTNMTWTGISNYVRMFQDVVFMQALENNFLYLIIQVPIMLLLALALASMLN
                     DRHLKFKGLFRTAIFLPCATSLVSYSIIFRSLFAVDGFVNTILIKLGILHTGYNFLTF
                     PTSARVVLILALIWRWTGYNMVFYLAGLQNIEYSVYEAAKIDGASPVQSFWRITVPLL
                     KPTILLTAIMSTNGTLQLFDESYNLTYGGPANATITMSHYIYNMSFKYVPNFGYAAAM
                     SYLILILVGTLAFIQMKVGDTRD"
     gene            899..1705
                     /locus_tag="PLU43_06345"
     CDS             899..1705
                     /locus_tag="PLU43_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28066.1"
                     /translation="MIKLKRALQYVILTIVSVLSVFPILWMAVSATNTSKDVIAGRML
                     PGTYLLENFKNLKASQNVGGAMLNSFKYSIILTLLSLLVCSLAGYGFEIFHDKAKDAV
                     MSVILLAMMVPFAATMIPLFQMFSKAGWLNSTIGFIMPTISTPFLIMMFRQSARSFPH
                     DIMEAARIDGLNETGIFFKMFFPTMKSTYAAAMTITFMNAWNSYLWPKVIMTDNNSIT
                     MPMLVANLKAGYMTDYGMLMLGVFLCSIPTVIIFFILQKSFAEGITGSVK"
     gene            1780..>2385
                     /locus_tag="PLU43_06350"
     CDS             1780..>2385
                     /locus_tag="PLU43_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOO28067.1"
                     /translation="MSGFDDKKIKDPVFFKENRIDAHSDHKYYRNNEEFEKDHSTFCF
                     SLNGLWKFNYAEKIGNAPVGFEAADFNCQGWDDIRVPAHIQLEGYDEPQYVNVQYPWD
                     GREDISPGEIPTAFNPTASYVKYFVLPENFDRKSTYISFQGVESGMALWLNGCYVGYS
                     EDSFTPAEFDLSPYLREGENKLAVQVYKWTAGSWCEDQDFFR"
BASE COUNT          616 a          459 c          601 g          709 t
ORIGIN      
        1 ggatggatga tggaccgggc aaagagagga cacttaagtc tgagtaaaaa acaaaatctg
       61 accggctgga tatttctggc gccggcagcg attctgatag cggcgatgag cttttatccg
      121 atgatcaagg cgctgctcct gtcgtttaaa accggtatcg gtacgaatat gacatggacg
      181 gggatcagca attatgtacg tatgtttcag gatgtggtat ttatgcaggc gcttgaaaac
      241 aattttttgt acctgatcat tcaggtgccg atcatgctgc ttcttgcgct ggcgctggcg
      301 tctatgctga atgacaggca tttaaagttc aaaggcctgt tccgtactgc gatctttctg
      361 ccgtgtgcga cttctcttgt ttcctattcg attattttcc gttcgctgtt tgcggtagac
      421 ggctttgtga atacgattct gatcaagctt ggaattttac atacgggtta taattttctg
      481 acgtttccga caagtgcgcg tgtcgttctt attcttgcac tgatctggcg ctggaccggt
      541 tataatatgg tgttttatct ggcgggtctt cagaatatcg aatattcggt ctatgaagcg
      601 gctaaaatcg atggggcgtc gccggttcag tccttttgga gaattacagt tccgctttta
      661 aagccgacga tcctgctgac agcaattatg tcaaccaatg gaacgttgca gctgtttgac
      721 gaatcctata atctgacata tggaggaccg gcaaacgcaa cgattacgat gtctcattat
      781 atttataata tgtcctttaa atatgtaccg aacttcggtt acgcggcagc gatgtcttat
      841 ttgatcctga tacttgtggg aacccttgca tttattcaga tgaaagtagg tgatacccgt
      901 gattaaattg aaaagagcac tgcaatatgt cattttaacg attgtttccg tactttccgt
      961 attcccgatc ctgtggatgg cagtatccgc aacgaataca agcaaggatg ttatcgccgg
     1021 cagaatgctt ccgggtacgt atctgctgga aaactttaaa aacctgaagg cttctcagaa
     1081 tgtcggcggt gcgatgctga actcctttaa atattccata atactgacac tgctttccct
     1141 gcttgtctgc tcgctcgcgg gatacggttt cgagatcttt catgataagg caaaggatgc
     1201 tgtaatgtcc gtgatcctgc ttgctatgat ggtgccgttc gcggctacga tgataccgtt
     1261 gttccagatg ttttccaagg ccggctggct gaattcgacg atcgggttta ttatgccgac
     1321 gatctccacg ccttttttga tcatgatgtt ccggcagagc gcacggtctt ttccgcatga
     1381 tattatggag gcggcaagga tcgacggatt aaatgaaaca ggtatttttt tcaaaatgtt
     1441 ttttccgaca atgaaatcaa cgtatgcggc agccatgaca attacgttca tgaatgcatg
     1501 gaattcctat ttatggccga aagttattat gacggataat aacagtatta cgatgccgat
     1561 gctggttgcc aatttaaaag caggatatat gacagactat ggaatgctga tgcttggcgt
     1621 tttcctctgc agcattccga ccgttattat tttctttatt ctgcagaaga gctttgcgga
     1681 agggattacc ggttccgtaa aatgagcgtt atctgatgaa acgatgccgg aaggcaggat
     1741 gaatggttta cataattaat aaacaaaggc ggaatgatga tgagcgggtt tgatgataag
     1801 aaaataaagg atcctgtgtt ttttaaggaa aacaggatcg atgcacattc ggatcacaaa
     1861 tattacagaa ataatgagga atttgaaaaa gatcacagta cgttttgctt ttcgcttaac
     1921 ggactttgga aatttaatta tgcggaaaag atcgggaacg cacccgttgg cttcgaagct
     1981 gcggatttta actgtcaggg atgggatgat atccgggtgc cggcacatat tcagctggaa
     2041 gggtatgacg aaccgcagta tgtcaatgta cagtatccgt gggacggaag agaggatatt
     2101 tcgcccggtg agattccaac agcgttcaat ccgacggcca gctatgtaaa atattttgta
     2161 ttgccggaaa atttcgacag aaaaagcacg tatatttcat ttcagggtgt ggagagcggt
     2221 atggcgctgt ggctgaacgg ctgttatgtc ggctatagtg aagacagttt tacgcccgct
     2281 gagtttgatt taagtcctta tctgcgagaa ggagaaaata agcttgccgt acaggtctat
     2341 aaatggactg cgggcagctg gtgtgaagat caggattttt ttcgt
//
LOCUS       DAOSYE010000325         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35203_length_2383_cov_2.518471, whole genome shotgun sequence.
ACCESSION   DAOSYE010000325 DAOSYE010000000
VERSION     DAOSYE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35203_length_2383_cov_2.518471"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..949)
                     /locus_tag="PLU43_06355"
     CDS             complement(119..949)
                     /locus_tag="PLU43_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO28068.1"
                     /translation="MENLKAVSFDLRKDVIDIIMAGGGGHTGGDMSVLDILMELYYEQ
                     MHISPSNMDKPDRDRFVLSKGHSVEAYYAVLCARGFLELDTVKREFSKFNTAYIGHPN
                     NKLPGIEMNSGSLGHGLPVCVGMALAGKLDKRDYRVYTVMGDGELAEGSVWEGVMSAS
                     HYKLDNLCAVVDRNCLQISGSTEDVMSHKRLKDRFESFDWHVRSVNGHDFKELKEAFE
                     EAKTVKGVPTMIIANTIKGYGSPVMENKAVWHHKVPTQEEYAQIMADLAERKEAALHE
                     "
     gene            complement(953..2353)
                     /locus_tag="PLU43_06360"
     CDS             complement(953..2353)
                     /locus_tag="PLU43_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOO28069.1"
                     /translation="MKKIKLGFAPTRRSIFSAPDAVKYANLTREKLRQMDVSFVDITD
                     INEEGLLYDESDRVRIAEKFRKEGVQGLFLPHCNFGTEYICARLAKELGVPVLLWGPL
                     DERPDEDGIRLRDSQCGLFATGKVLRRFQVPFTYLTNCRLSDPVFERGLNDFLAVCNV
                     VHTFKNTRILQIAPRPFDFWSTMCNEGELLEKFNIQLSPIPMPELTGELKKAKEEGTE
                     VKEVAAYCRSNMDVKIKDEELENVAALKVAMKRLAGKYGCNAIAIQCWNALQGEIGIM
                     PCAANSLLNEEGIPVVCETDIHGAITAVMAEAAGMNEIRGFFADWTVRHPDIANGELL
                     QHCGPWPISVAKEKPSIEYPLAFDYPGAVAAEAKHGDMTLCRFDGDNGEYSLLLGKAK
                     GVDGPYTKGTYVWVEVENIKRLEAKIVEGPYIHHCVGIHRDVVPVLYEACKYIGIRPD
                     LYDPVEEEVKAYLRGE"
BASE COUNT          576 a          685 c          452 g          670 t
ORIGIN      
        1 ggattcggat gagccgatgg ggtctacaac gagggaatca ctgcataaaa cgataatatc
       61 cttatcttcc tttgctttat taagcagtac attacaaata gctgccctgt tcggaatttt
      121 attcatgaag agccgcctcc tttctttctg caagatccgc catgatctgt gcgtattctt
      181 cctgcgtcgg caccttatga tgccataccg ccttattttc cattaccggg gagccgtatc
      241 ccttgatcgt attggcaatg atcattgtcg gaactccttt cactgttttg gcttcctcaa
      301 atgcttcctt taattcttta aaatcatgcc cgttcacact gcgtacatgc cagtcaaagc
      361 tctcaaaccg gtctttcagc cgtttgtgag acattacatc ctccgtactt cctgaaatct
      421 gaagacagtt gcggtccacg accgcacaca gattgtcaag cttataatgg gacgcagaca
      481 taacgccttc ccacacggag ccttccgcaa gctccccgtc tcccatgacc gtataaacac
      541 ggtaatccct cttatctaat tttccggcca gcgccatgcc gacacagacc ggaagtccgt
      601 gtccgagaga acccgaattc atctcgattc caggcagctt attattcgga tgtccgatat
      661 aggccgtatt aaatttggaa aattcccttt tgaccgtatc aagctccaaa aatcctcttg
      721 cacataaaac agcataatag gcctcgacag aatgcccttt gctgagcacg aaccggtctc
      781 tgtccggttt gtccatattg gacggagaaa tatgcatctg ttcataatac agttccatca
      841 aaatatcaag gacactcata tctccgccgg tatgtccgcc gcctcccgcc ataatgatat
      901 ctatgacatc ctttcttaaa tcaaatgaaa cggcctttaa attttccatt tctcattctc
      961 ctctcaaata cgctttcact tcttcttcga ccggatcata cagatcaggc cggatcccaa
     1021 tatatttaca tgcttcatac agcaccggca caacatcccg atggatcccg acacaatgat
     1081 ggatataagg accttctacg atcttcgcct caagccgctt gatattctcc acttccaccc
     1141 agacgtatgt tccttttgta tatggtccgt caacaccctt tgctttgcca agaagcagag
     1201 aatactctcc attgtcgccg tcaaatctgc acagcgtcat gtctccgtgc ttagcttccg
     1261 ccgcaaccgc acccggatag tcaaatgcaa gcggatactc gatcgaaggc ttttcctttg
     1321 ccacggagat cggccatgga ccgcagtgct gcagcagctc tccatttgca atatccggat
     1381 gtctgaccgt ccagtctgca aaaaagcccc ggatctcatt cattccggca gcctctgcca
     1441 tgaccgcggt gatcgctccg tgtatatccg tctcacatac gaccggtatt ccctcttcat
     1501 tcagaaggga attcgccgcg cacggcataa taccgatctc tccctgcagt gcgttccagc
     1561 actggatcgc gatcgcatta cagccgtatt tgcccgcaag ccttttcatt gcgactttca
     1621 gagccgctac attttcaagc tcttcatctt tgatcttgac atccatattg cttctgcaat
     1681 acgcagctac ttctttgact tccgttcctt cttcctttgc ttttttcaat tcaccggtaa
     1741 gctccggcat cgggatcggg gaaagctgga tattgaattt ctcaagcagt tctccctcgt
     1801 tacacatcgt agaccaaaaa tcaaacggac gcggtgcgat ctgaaggatc cttgtgtttt
     1861 taaatgtatg gacaacattg cacacggcaa gaaagtcatt aagaccacgc tcaaaaaccg
     1921 gatccgaaag cctgcagttt gtcagatacg taaagggcac ctgaaatctc ctcagtactt
     1981 ttcctgttgc aaacagtccg cactggctgt cacgcagccg gatcccatct tcatccggtc
     2041 tttcgtcaag cggtccccat aaaagtaccg gcacacccag ttcctttgca agccgcgcgc
     2101 agatatactc tgttccgaaa ttgcaatgcg ggagaaacag accctggact ccttcttttc
     2161 tgaatttttc ggcgatcctg acccgatcgc tctcatcgta aagcagtcct tcttcattga
     2221 tgtccgtaat atcaacaaac gaaacatcca tctgcctgag tttttccctt gtcaggtttg
     2281 catatttgac tgcatccgga gcactgaaaa tgcttcttct tgtcggcgca aaaccgagtt
     2341 tgattttctt catattctga taccctccac tttcctcaaa gaa
//
LOCUS       DAOSYE010000326         2379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35297_length_2379_cov_3.440620, whole genome shotgun sequence.
ACCESSION   DAOSYE010000326 DAOSYE010000000
VERSION     DAOSYE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35297_length_2379_cov_3.440620"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..321)
                     /locus_tag="PLU43_06365"
     CDS             complement(16..321)
                     /locus_tag="PLU43_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28070.1"
                     /translation="MSIFKKINYIFSKKQKVQSVLLCVGLFVGALLELVGVSFITQLV
                     SLITAPEKIHTSAMLQSFYDLFHMSSDGQFFLYIVIALILVYVVKNNCLSEDIWNRS"
     gene            complement(336..2201)
                     /gene="asnB"
                     /locus_tag="PLU43_06370"
     CDS             complement(336..2201)
                     /gene="asnB"
                     /locus_tag="PLU43_06370"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO28071.1"
                     /translation="MCGIFGVINEKIEKELAEQCVNRIRHRGPDGCGLWQEGGITLGH
                     RRLAILDLSANGSQPMAYADGRYMLVFNGEIYNFIEIRRELEQKGYRFRSETDSEVVL
                     ASYMEWGEKCLDRFNGMWAFAIWDRNEKRMFLSRDRFGVKPLYYTKLQHKNAIAFGSE
                     MKALLPLMKEVKPNRELVTDPGRTVFYESTDACVIEGISRIPAGSYAYIDESGMRITR
                     WWNTLDHLVQVPDRYEEQVELMRELFLDACKLRMRSDVTIGTALSGGLDSSATICAMA
                     HLARNDSDVRMNEDWQHAYVAVFPGTTMDESAYAKKVTDYLGINSTFVEIDPKKAIGM
                     LDTYIYMFEDVYLTSPIPMMMLYHALRSDGTLVTIDGHGADELFGGYTFDFIHALRDA
                     GSRQERELILTAYTDSFPQDDSNIALASRAKSSVYLHYLKRELKDRLNRKKEDYKTVR
                     AAADPRYGQLDTLNKILYASSHETILPTLLRNYDRYSMASGIEIRMPFMDYRIVELAM
                     SLGWRSKLHGGYSKSIIRDAMAPYMPKDIAYRKTKIGFNTPIVEWMKGPLKEYFEDTI
                     RSGEFLSCDLIDPKKTMEQVTRVITDSSATFAMGEQAWSSLYPYLWERAFVKNQY"
BASE COUNT          600 a          641 c          466 g          672 t
ORIGIN      
        1 gatgctgcag tcattttatg atctgttcca tatgtcttcg gacagacagt tatttttgac
       61 cacataaacc aggatcaggg caattacaat atacaaaaag aactgtccgt ccgaagacat
      121 atggaacaga tcataaaatg actgcagcat cgcactcgta tgaattttct ccggtgccgt
      181 aataagcgaa acaagctgcg tgataaaaga cacaccgaca agctccagca gtgccccgac
      241 aaacaaaccg acacacagca gcaccgactg cactttctgc ttttttgaaa aaatgtaatt
      301 gattttttta aaaatactca tcaggttctc ctgtatcaat actgattttt aacaaatgcg
      361 cgttcccaca aatacggata cagactgctc cacgcctgtt ctcccattgc aaaggttgcg
      421 ctgctgtcgg taatgacccg cgtaacctgt tccattgttt ttttcggatc gatcagatcg
      481 caggaaagaa attctccgct gcggatcgta tcttcaaagt attccttcag cggtcctttc
      541 atccattcaa cgatcggcgt attgaatccg atctttgttt tgcggtatgc aatatccttt
      601 ggcatatatg gtgccatggc atccctgata attgattttg aataaccgcc gtgcagcttg
      661 gatctccagc caagcgacat ggcaagctcc acgatccggt aatccataaa cggcatcctg
      721 atctcaatgc cgcttgccat gctgtagcgg tcatagttcc gcaaaagcgt cggcaggatc
      781 gtttcatggg aagaggcata taaaattttg ttcagcgtat caagctgccc ataacggggg
      841 tcagctgccg cccgtactgt cttatagtct tcttttttgc ggttcagccg atccttcagt
      901 tcccgcttca gataatgaag ataaacactt gattttgccc ggctcgccag tgcaatattt
      961 gagtcatcct gcggaaaact gtccgtatag gcagtcagga tcagctctct ctcctgcctg
     1021 ctgcctgcat cgcgcagtgc atggataaaa tcaaacgtat agccgccaaa cagctcatcc
     1081 gcgccgtgcc cgtctatcgt aacaagcgtt ccgtcgctgc gcagcgcatg ataaagcatc
     1141 atcatcggaa tcggactggt cagataaaca tcttcgaaca tataaatata ggtatccagc
     1201 atgccgatcg cttttttcgg atcgatctcc acaaaggtac tgttgatccc aagataatcc
     1261 gtcacttttt tagcataagc gctttcatcc attgttgtac ccggaaatac tgccacatat
     1321 gcatgctgcc agtcttcatt catacggaca tcgctgtcgt ttcgcgccag atgtgccatt
     1381 gcgcagatcg ttgcggaaga atccagtccg cccgagagcg ccgtaccgat cgtcacatca
     1441 cttctcattc gcagtttaca ggcatcaaga aacagttccc gcatcagctc cacctgttct
     1501 tcataccggt ccggcacctg caccaaatga tcaagcgtat tccaccatct tgtgatccgc
     1561 atgccgcttt cgtcaatata ggcataggat cctgccggaa tacggctgat cccttctatg
     1621 acacaggcgt ctgtactttc atagaacacc gttctgccag gatcggttac aagctccctg
     1681 ttcggtttta cttccttcat caagggaagc agtgccttca tttccgaacc gaacgcgatc
     1741 gcatttttat gctgaagctt tgtataatac aaaggcttta ccccaaaccg gtcccggctt
     1801 aaaaacatcc gtttttcatt tctgtcccaa atagcaaatg cccacatgcc gttgaacctg
     1861 tcaagacatt tttcgcccca ctccatatag gaagcaagca cgacttcaga atctgtttca
     1921 cttctgaacc gataaccctt ttgctccagt tcccttctga tctcgataaa attatagatc
     1981 tcaccgttaa acacgagcat gtatctgccg tccgcatatg ccatcggctg cgaaccattg
     2041 gcagaaagat ccaggatcgc aagccgcctg tgtccgagcg taatgccgcc ctcctgccac
     2101 agaccacatc cgtccggtcc ccggtgcctg attctgttca cacactgttc agccagttct
     2161 ttctctattt tttcgttaat aactccgaat atgccacaca taatttttgc agttcctttt
     2221 cattgcctgt aaagcttctg ctttacacga tagaataata tcgtacacag ggatctaact
     2281 ttcagtgctc tgtaataata aagatatttc tttgcttcct tttttttcgg cagagctccc
     2341 gttgtttttg tatcatataa ataaaattca tttccgggt
//
LOCUS       DAOSYE010000327         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35412_length_2375_cov_2.225000, whole genome shotgun sequence.
ACCESSION   DAOSYE010000327 DAOSYE010000000
VERSION     DAOSYE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35412_length_2375_cov_2.225000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..759)
                     /locus_tag="PLU43_06375"
     CDS             complement(70..759)
                     /locus_tag="PLU43_06375"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOO28072.1"
                     /translation="MAVNIILIVSFSYAALLSAVMGVYTLQINKKLPQYQLFFAASVS
                     LSLWALSFAMFENAASLDQAFMWRRIGAVGIRFFYVFLLDFLIRLIYGRLSKRGRILE
                     YLMYIPSVILIYVYTVSNRWSSEQFRIIRTSYGWTTGSVQNFWERFFYLYYFGYVFIG
                     IVLLVMWYKKSEDVRNKIIARNILFSFLITYVLGSFTDVIANITEDVIFPEIGPILAM
                     LQPTSARTKPA"
     gene            complement(918..1328)
                     /gene="mscL"
                     /locus_tag="PLU43_06380"
     CDS             complement(918..1328)
                     /gene="mscL"
                     /locus_tag="PLU43_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOO28073.1"
                     /translation="MKNEEKIEKGKGFLAEFKKFITKGNVLDMAVGIIIGSAFTAIVT
                     SLVNDVIMPVIGLILGGVDFSALSVQVGDATILYGSFIQAVVNFILIALTVFILLKAI
                     NQVIEKKEEQKPVPKPSEEAVLLSEIRDLLKEQK"
     gene            1603..1773
                     /locus_tag="PLU43_06385"
     CDS             1603..1773
                     /locus_tag="PLU43_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO28074.1"
                     /translation="MTQQELAEKVGVSRQTIISIEAGKYSPSLQLAFLIADAFDVKID
                     EAFDYEVKGENP"
     gene            1770..2162
                     /locus_tag="PLU43_06390"
     CDS             1770..2162
                     /locus_tag="PLU43_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28075.1"
                     /translation="MKAIVIVSSCHHHNTEKVGQSIASVLNAPIITPQQISPQELTGY
                     DLIGFGSGIYGGMHHRNILDLTDTLLRTDGAKAFIFSADRGPRFLVENESFLQNKTQK
                     DHKRDIKLLGNSTAPDSIPTVFRSFSAE"
BASE COUNT          747 a          562 c          399 g          667 t
ORIGIN      
        1 ggttcgagga ttgatcctcg cgcggctaaa tcacccgccc gccgtcgacc tccatcgcaa
       61 cgccggtgat catgctggct tcgtccgagc agaggtaggc tgcagcattg ccaatatcgg
      121 cccaatttcc gggaaaatga catcttctgt tatatttgca ataacatccg taaaactgcc
      181 caggacataa gtaatcaaaa acgaaaacaa aatattgcgt gctattattt tattccttac
      241 atcttcactt tttttatacc acattaccaa aagaacgatt ccaataaata catagccaaa
      301 ataatacaaa taaaaaaatc gttcccaaaa attctgaaca gaacctgtcg tccaaccata
      361 tgacgttcta ataattctaa attgttccga actccaccga tttgaaactg tatatacgta
      421 aatcaaaatc acagacggga tatacatcag atattcaagt atccttcccc gcttggaaag
      481 cctgccatag atcagcctga ttaaaaaatc cagcaaaaaa acataaaaaa atctgatgcc
      541 gaccgcaccg atccttctcc acataaatgc ctgatccaat gatgctgcat tttcaaacat
      601 ggcaaaactg agtgcccaga gactcaaaga cacagatgct gcaaagaaca attgatactg
      661 cggcagtttt ttattaatct gcagcgtata aacgcccatt acagcactta gcagcgccgc
      721 atatgaaaaa cttacaatta aaattatatt tactgccatg atttatcccc tcaataacaa
      781 gacacgtttt accctatacc caattttgta tattatatca ttttatatac tatctgtcat
      841 tgtactttac tcaaaaaaac agaggaaaca tcaactaaaa gacgtttccc ctgcttgtat
      901 tttcgttcct catgatccta tttctgttct tttaaaagat cacgaatttc tgacagcagc
      961 acagcctctt ccgaaggctt gggcactggt ttttgttctt cctttttttc tatcacctga
     1021 ttgattgcct ttaacaaaat aaatacggta agagcgatta gaataaaatt cacaacggcc
     1081 tggataaagg aaccatataa tatggttgcg tctcccacct gcacagacag cgctgaaaaa
     1141 tcaacacctc ccaatatcag tccaatgacc ggcatgatca catcattcac aagggatgtc
     1201 acgatcgcgg taaacgcgct cccaataatg atgccgacag ccatatccaa aacattgcct
     1261 tttgtaatga attttttaaa ttctgccaga aaccctttcc ccttttctat cttttcttca
     1321 tttttcatcg acctgctacc atccttttct cttctttctt tattcggctt ttcctaactt
     1381 ctatattata taatacttat acggaaaata cagtcacctt gcaaaaatta ttttttgttt
     1441 tcagacaggg caattatgct cactatcctg ttcctggcat tcggcacagg ctcactagca
     1501 ggagaaacac tgcgtatcta ctattatagg gctggtatca aataatgaaa aaaaaataat
     1561 gatcacaaac aatatcagaa ggcttcgttt tttgcaaacg aaatgacaca gcaggaattg
     1621 gctgaaaaag ttggcgtctc cagacagaca attatttcga tcgaggcagg caaatactca
     1681 ccgtcattac agcttgcctt tctcatcgca gatgcattcg acgtaaaaat cgatgaggca
     1741 tttgattatg aagtaaaagg agaaaaccca tgaaagcaat cgtgatcgtc agttcctgcc
     1801 atcatcataa cactgaaaaa gtcggtcaaa gcattgcatc tgttctcaat gctccgatca
     1861 tcactccaca gcagatctcg ccgcaggaac taaccgggta tgatctgatc ggctttggct
     1921 ccggcatata cggtggtatg caccatagaa atattttaga tctgactgat accctgctcc
     1981 ggacagacgg agcaaaagct tttatttttt cagcagacag gggccctcgt ttccttgtgg
     2041 aaaacgaatc tttcctgcaa aataaaacgc aaaaagatca taaaagggat atcaaattgt
     2101 tggggaattc aactgcgccg gactcaatac caacagtttt ccgaagcttt tcggcggaat
     2161 aaacaaaggc cggccggata acagagatct ggcactggct gcggacttcg cccggaacct
     2221 atctatcagt tgattttatg aaatgatatt taaatatttt aaagcctgat aagtgctgcc
     2281 gaaatctaca ctgccgcata cataatcggc cgttgcccgt tccttcatat aggtatcctc
     2341 catcatttca aaggtagtgt caaatctact acctt
//
LOCUS       DAOSYE010000328         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35566_length_2368_cov_2.967142, whole genome shotgun sequence.
ACCESSION   DAOSYE010000328 DAOSYE010000000
VERSION     DAOSYE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35566_length_2368_cov_2.967142"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="fliB"
                     /locus_tag="PLU43_06395"
     CDS             complement(<1..427)
                     /gene="fliB"
                     /locus_tag="PLU43_06395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HOO28076.1"
                     /translation="MKHWKFKPNYYDQFVCIADSCPMTCCQEWKITIDGPTLEKWKQI
                     PPPSDTKPSYQSLAAYTTIQEGSSVISLNRDRRCPFLQKDQLCSLVLSYTDQILSETC
                     SQFPRQFRQFSDRTEYSLAPCCPEVIRLMNETDAVTILRT"
     gene            593..1435
                     /locus_tag="PLU43_06400"
     CDS             593..1435
                     /locus_tag="PLU43_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOO28077.1"
                     /translation="MIKILYIDQIQELESDIRPNILDYIRQRQMGNFETFENFSVMAF
                     DWYDVAQRQRETHQVFIYCSVKDLFFLCEDAVMEHKVQKTMEEERGEAGENTSLLLYR
                     FFARLLKNDQLYLDRVETEITDTEDEMLTGSRKEYIKKIIEYRKELLSLKSYYEQLDS
                     IFDELSANDNSLLEADGIRRFHILSNRTDRCINEVQNLREYVSQMREAYQSTVDIQQN
                     ELMKLFTVITTIFLPLTLLVGWYGMNFPMPEFQWNYGYPALIVVSIIIVAILLYIFKK
                     KKWL"
     gene            1509..>2368
                     /locus_tag="PLU43_06405"
     CDS             1509..>2368
                     /locus_tag="PLU43_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOO28078.1"
                     /translation="MVQKKFDISGMQCAACSSAVERVTGKLAGVEEHSVNLTTGILTI
                     SFDEKALSDEDIIKKVERAGFGAAVHIDRNRQERKEQEERIRSEVEKTRKQLTVNAIL
                     AVLLLYVSMGHMLPFLLPLPKIIDMQENPMSFAVVQLILTVIILFNGRRFYTSGFQNL
                     FKGHPNMDSLVAIGTGSAFLYSFVMTVTIPQTAAHVHHLYYESAALVVTLVMVGKYME
                     GRSKNKTSDAIRKLMELAPETALVIRDGKQTEVLAGQVQAGEYLLIKPGSRIPLDGTI
                     ISGETTVDES"
BASE COUNT          673 a          453 c          617 g          625 t
ORIGIN      
        1 ccgtgcgcag gatcgttacg gcatctgttt cgttcataag tcgtatgact tccggacagc
       61 agggagcaag ggaatactcc gtccgatctg aaaactgacg gaactgtctt ggaaactgtg
      121 aacaggtttc ggataatatt tgatcggtat aggaaagaac cagactgcat aactggtctt
      181 tttgaagaaa ggggcatctg cggtccctgt tcagggaaat gacagatgag ccttcctgta
      241 tggtcgtata ggctgcaagt gactgatatg acggctttgt atcggaaggc ggcggaatct
      301 gcttccattt ttccagagtc ggtccgtcta tggtgatctt ccattcctgg cagcaggtca
      361 tgggacagct gtcggcaata cagacaaact gatcatagta atttggtttg aacttccaat
      421 gtttcacgaa tattctcctt ttataacggt ttcgtatgat tcagtgtaat gttttttggc
      481 agccgtgtca aatcagaaga tgaaaaatct tacaggcagt attccaattt ttctaagaat
      541 gcgatagaat aaaaacaatt gagaagtcat ataaaaagcg ggagatgatc gcatgataaa
      601 aatactgtat atcgatcaga tacaggaact ggaatctgat atcaggccaa atattctgga
      661 ctatatcaga cagcgccaga tgggcaattt tgagacattt gaaaatttca gcgtgatggc
      721 gtttgactgg tatgatgttg cacaaagaca gcgggaaacg catcaggtct ttatttactg
      781 cagtgtaaaa gatctgtttt ttttgtgtga agatgccgtg atggagcata aagtgcagaa
      841 gaccatggag gaggaacggg gagaagcagg ggagaatact tccttgctgt tgtatcgttt
      901 ttttgccaga ctgctgaaaa atgaccagct gtatctggac cgggtcgaaa cggaaattac
      961 ggacactgaa gatgaaatgc tgacaggttc caggaaagaa tatataaaga agatcattga
     1021 atacagaaaa gaactgctga gtctgaaaag ctattatgag cagctggatt cgatttttga
     1081 tgagctgtcg gccaatgaca acagcctgct ggaagcggac gggatccgcc gtttccatat
     1141 actcagcaac cgcacggacc gctgtatcaa cgaagtgcag aatctgcgcg agtatgtttc
     1201 ccagatgcgg gaagcatacc agtctacggt tgatatccag cagaatgaac tgatgaagct
     1261 gttcacagtc attacgacta ttttcctgcc tctgacgctg cttgtcggct ggtatggcat
     1321 gaattttcca atgcctgaat ttcaatggaa ttacggatat ccggcattga tcgttgtcag
     1381 tattatcatt gtggctattc tgctgtatat tttcaagaaa aagaaatggc tgtgaacata
     1441 tcgcttgaca aatactgtgt gaactgataa gatcaatata ccccatgggg gtataaggag
     1501 gcgatcgaat ggttcaaaaa aagtttgata tatcgggtat gcagtgtgcc gcatgctcca
     1561 gtgcggttga acgtgtgaca ggaaagcttg cgggtgttga ggaacattct gtgaatctga
     1621 cgacgggaat actgacaatt tcgtttgatg agaaggctct gtccgatgaa gatattataa
     1681 aaaaggtcga gagagccggc tttggtgcag cggtgcatat agacagaaac aggcaggagc
     1741 gcaaagagca ggaggaaagg ataagatccg aagttgagaa aacaagaaaa cagctgactg
     1801 taaatgccat tctggctgtg ctgctgctgt atgtttccat gggacatatg ctgccgtttt
     1861 tgctgccgct gccaaagatc atcgatatgc aggaaaatcc aatgtctttt gcggtcgtgc
     1921 agctcatcct gacagttatt attttgttta acggaaggcg gttctatact tccggatttc
     1981 agaatctgtt caaagggcat cccaatatgg attcgcttgt tgcgatcggt accggaagcg
     2041 cttttttata tagctttgta atgacggtca cgataccaca aacggctgca catgtgcatc
     2101 atctttatta tgagtctgca gcgctcgttg taacgcttgt catggtcggc aaatatatgg
     2161 aaggccgcag taaaaacaag acatctgatg cgatccgcaa actgatggag ctggcgcctg
     2221 aaacagcgct cgttatccgt gacggaaaac agacagaggt tctggcagga caggtacagg
     2281 caggcgaata tctgctgatc aagcccggca gccggatccc gcttgacggt acgatcattt
     2341 cgggagagac gacagtggat gaatccat
//
LOCUS       DAOSYE010000329         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35567_length_2368_cov_2.954604, whole genome shotgun sequence.
ACCESSION   DAOSYE010000329 DAOSYE010000000
VERSION     DAOSYE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35567_length_2368_cov_2.954604"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLU43_06410"
     CDS             <1..873
                     /locus_tag="PLU43_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HOO28079.1"
                     /translation="RSIAATAANRGYLTSAVDQIEAGTLKKYKYFFDQTIYANRVFDS
                     HGVADKSVEVKFGPNIKDWPKMGALPENLVLKVVSEIHDPVTTTDELIPSGETSSFRS
                     NPLGLAEFTLSRKDPAYVGLAKEIQKAEKERTEGGHPSQTNAEIKPVMEVIRKTFDSA
                     DDTNTGIGSTIFAVKPGDGSAREQAASCQKVLGGWANIATEYATKRYRSNLINWGMLP
                     FLIDAGELPFKNKDFLFIPGIRKAVEEKWEEIKAYAVSDTLREFTLRLGELTDDERTI
                     ILKGCLINYYADQA"
     gene            1088..2149
                     /locus_tag="PLU43_06415"
     CDS             1088..2149
                     /locus_tag="PLU43_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28080.1"
                     /translation="MVVNSSSIGMESARKYTSVRMEAYSFGTYKKTGNIESTGNGEND
                     NAEAEKSSENKTGSLWDSMEDLMNRYEQTKVRSVSSTKKEQDAIANIRMRCLQYLLRI
                     LFGSKSKRDVLSDEDIVTELQGQNSQSVSTSVSSYTKIDYYGEAEETAFSTEGTVVTA
                     DGRELSFNLSLEMSRSFEAYYSESYEIEQDLCDPLVINLDTDIAEVSDQKFMFDIDAD
                     GITDEISKLDAASGYLALDVNGDGEINDGSELFGTKSGDGFKDLSAYDSDENGWIDEA
                     DEIWDRLLIYTQDEEGNSVTYGLSEKGVGAICLKNVDTEYSLTNASENQVNAVIRKTG
                     IFLYENGNVGTVQHLDIAK"
     gene            complement(2223..>2368)
                     /locus_tag="PLU43_06420"
     CDS             complement(2223..>2368)
                     /locus_tag="PLU43_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO28081.1"
                     /translation="TRSRRPVTLVYYEKFNTKEEAMKREYAIKQLSSDQKQRLIDSVS
                     NML"
BASE COUNT          706 a          433 c          636 g          593 t
ORIGIN      
        1 cgttcgattg cggcgaccgc tgcgaacagg ggatatctga catctgccgt ggatcagata
       61 gaggccggta cattgaaaaa atacaaatat ttcttcgatc agacaattta tgcgaaccgc
      121 gtatttgaca gtcatggagt tgccgataaa agcgttgagg tcaaatttgg accgaatatc
      181 aaggactggc cgaagatggg tgcattgccg gaaaatctcg tgctgaaggt cgtatcggaa
      241 attcatgacc cggtgacgac aacggatgag ctgattccat caggagagac ttcttcgttc
      301 cgctctaatc cgttaggact tgcggagttt accctttcca gaaaggaccc ggcttatgtg
      361 ggtctggcaa aagagatcca gaaggccgaa aaggaaagaa cggaaggcgg acatccgagt
      421 cagacaaatg ccgaaatcaa accggtaatg gaagtgatca gaaaaacttt tgacagtgca
      481 gatgacacaa atacgggtat tgggagtacg atctttgctg tgaagcccgg tgacggttct
      541 gcgcgtgaac aggcggcgtc ctgtcagaag gtacttggcg gctgggccaa tatcgcaact
      601 gaatatgcga caaaaagata ccggtcaaat ctgatcaact gggggatgct tccgtttttg
      661 atcgatgcgg gcgaactgcc gtttaaaaat aaagacttct tatttattcc cggaatcaga
      721 aaagctgtgg aagaaaaatg ggaggaaata aaagcatatg cggtatccga tacgctcaga
      781 gagttcacgc tgcgtctcgg agaactgacg gacgatgaaa ggacgattat attaaagggt
      841 tgtctgatta attattatgc ggaccaggca taataaaatt gtatcagcta ttatattttt
      901 taccggattg ccgataatta ttatacagga tgagatcttg gccggatcaa tattctgaat
      961 agtatggctg accacggagg gtatgacatg aacaggaatg gaattctgtt tatgaaaatg
     1021 ccctcttttt tatttacgac acggagggag tgatagaaag tgtgctaggc cgcatagggg
     1081 gaatactttg gttgtaaaca gcagcagtat aggaatggaa tctgccagaa aatatacttc
     1141 tgtgcgcatg gaagcgtatt ctttcggcac ttataaaaaa accggcaata ttgaaagcac
     1201 tggaaatgga gaaaacgata atgcggaggc agaaaagtcc tccgaaaaca aaacgggaag
     1261 cctgtgggat tctatggaag atctgatgaa ccggtacgaa cagacaaagg tgcgcagtgt
     1321 ttcaagcaca aaaaaggagc aggacgccat tgcgaacatc agaatgagat gcctccagta
     1381 cctgttacgg attctgtttg gttcaaaaag caagcgggat gttctttcgg atgaggatat
     1441 cgttacggaa ctgcagggtc agaattccca gagcgtttcg acgtcagtaa gcagttacac
     1501 aaagatcgat tattacgggg aagcagagga aactgctttt tctaccgaag ggacggtcgt
     1561 aactgcggac ggcagagagc tttccttcaa tttaagtctt gagatgagcc gcagctttga
     1621 agcgtattat agcgaaagct atgagatcga acaggatctg tgtgatccgc ttgtgattaa
     1681 tctggatacg gacattgccg aagtttcgga tcagaaattt atgtttgata tcgatgcgga
     1741 tggtattact gatgagatct cgaaactgga tgctgcgagc ggatatctgg ctcttgatgt
     1801 gaacggggac ggagagatca acgatggaag cgaattgttt ggaacaaaat ccggagatgg
     1861 ctttaaagat ctgtcagcgt atgattcgga cgaaaacggg tggatcgatg aggccgatga
     1921 gatctgggac agacttctga tctatacgca ggatgaagaa ggcaacagcg tgacctatgg
     1981 tctgtctgaa aaaggcgttg gtgcgatctg tctgaaaaac gtggatacgg aatattctct
     2041 gacaaatgct tccgaaaacc aagtgaatgc ggttatccgg aaaaccggca tttttctgta
     2101 tgagaatgga aatgtcggca cagtgcagca tttggatatt gcaaaatgaa tggaaacaga
     2161 aaattttatt gaaaaaaaga ctgggatcgg cgccgattcc ggtctttttt tgagcatcgg
     2221 ttttacagca tattcgaaac ggagtcgatc agccgctgtt tctgatctga ggacagctgc
     2281 ttgatcgcat attctctttt cattgcctct tcctttgtat taaatttttc ataataaaca
     2341 agtgtgacag gacgtctgga acgtgtat
//
LOCUS       DAOSYE010000330         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35574_length_2368_cov_2.080415, whole genome shotgun sequence.
ACCESSION   DAOSYE010000330 DAOSYE010000000
VERSION     DAOSYE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35574_length_2368_cov_2.080415"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLU43_06425"
     CDS             complement(<1..638)
                     /locus_tag="PLU43_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO28082.1"
                     /translation="MKKDITIGELADLMDVSTHQIRYFEEKGILFPAYIDTNGYRMYG
                     TEQIYILSHILLLRRLHIPVSDIKKQFTSFSPDDYLNMMEKSILKIEEQIKTLQYLRN
                     VIAGIMEKAKSNQKYINIFSIKSLPKRHLSLIKKTDRTFSFHARNIYDLLYKTNKTDS
                     VYRSDFITLIDGQTLHICIGACDSKDYILREGSYLSFQFLIKEEKELEHRIE"
     gene            757..1377
                     /locus_tag="PLU43_06430"
     CDS             757..1377
                     /locus_tag="PLU43_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO28083.1"
                     /translation="MDQDIKIRRFQEEDYEAVIKILVYSFKSKFHALTNLSDEEISQL
                     LRDTGFIYHIPFEGYLVGEAGGSVQGVMLLKWNRQRRPVRKGLRFFELTGKYGALNVC
                     KFIFGLMILQEKLDEDECYIEHVAVNPEARGWGLGTMFLDYGKELIGKTAYLDKLTLH
                     VAVNNKKAFSLYKQQGFQVKMLQSSFFTFLLLKEREWYYMNLRNIK"
     gene            complement(1436..1852)
                     /locus_tag="PLU43_06435"
     CDS             complement(1436..1852)
                     /locus_tag="PLU43_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO28084.1"
                     /translation="MTISARNKIAGSVASIKEGVVNASVIIETANGDKISSTITKESA
                     KELGLAVGKEAIAVVKASEVMIGIGEMKISARNQIDCTVTDITEGMVNALVSLTAEGG
                     SCLSSNISMAAVKELGIEKGSKVKAVIKATSVMVSV"
     gene            2213..>2368
                     /locus_tag="PLU43_06440"
     CDS             2213..>2368
                     /locus_tag="PLU43_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO28085.1"
                     /translation="MLRNGGKNMRQVAIYGKGGIGKSTTTQNLTAGLADMGKHIMIVG
                     CDPKADST"
BASE COUNT          717 a          412 c          499 g          740 t
ORIGIN      
        1 tgttcgatcc tgtgctccag ttctttttct tcttttatca gaaactgaaa ggaaaggtag
       61 gagccttctc tcagaatata atctttggaa tcgcaggccc caatacagat atgcaaagtt
      121 tgcccgtcga tcagcgtaat aaaatcactg cgataaacag agtctgtttt gttcgtttta
      181 tataatagat cataaatatt tctggcatgg aaagaaaaag tccgatcagt tttttttatg
      241 agggacagat gtcttttagg gaggcttttt atgctgaaga tatttatata tttctggttt
      301 gattttgcct tttccataat cccggcaatt acatttctaa gatactgcag tgtctttatc
      361 tgctcctcaa ttttcagtat tgatttttcc atcatattca aataatcatc cggtgaaaaa
      421 gaagtgaact gttttttgat atcagagaca ggaatatgca gtcttcttaa taataaaatg
      481 tgggaaagga tgtatatttg ttcagtacca tacatccgat atccgttcgt atcaatatat
      541 gctggaaata aaattccttt ttcttcaaaa tatcggatct gatgagtgga aacatccatc
      601 agatctgcca gttctccaat cgtaatatcc tttttcatta tataatattc tcctattgac
      661 attaggtcag acctaagctt taaactgagt atacaatgat gagcagatcc gatcaagtgt
      721 attgaaatta aaatgtttta atggaaagga agaaaaatgg atcaagatat taagatcaga
      781 aggtttcaag aagaggatta tgaagctgtc ataaaaatat tggtatattc ttttaaaagt
      841 aaatttcatg ccctgacgaa tttatctgat gaggaaatat cacaattatt aagagataca
      901 gggtttattt atcatattcc ctttgaagga tatttggtag gagaagcagg tggcagcgtg
      961 cagggtgtca tgctgcttaa atggaacaga caaaggcgtc ctgtgagaaa aggtctcaga
     1021 ttttttgaac tgaccggaaa atatggagca ttaaatgtat gcaaatttat ttttggtctt
     1081 atgattctgc aggaaaagct tgatgaagac gaatgctata tcgaacatgt agcagtcaat
     1141 cctgaggcca ggggatgggg gctcggaacc atgtttttgg attatgggaa ggaattgatc
     1201 ggtaaaacag cgtatttgga taagcttacg ctgcatgttg cggtgaataa taaaaaagcc
     1261 ttcagcctgt ataaacaaca ggggtttcaa gttaaaatgc tgcaaagcag tttctttact
     1321 tttttattgc tgaaagagcg ggagtggtat tatatgaact tacgaaatat aaaataagct
     1381 gccgcgagac gtttcagtct tacgacagct tatttaatag ccggctttta gaaagctata
     1441 cggataccat aacggaggtc gccttgatga cagctttgac tttggagcct ttttcaatgc
     1501 caagttcctt tacggctgcc atggaaatgt tggaggaaag acagctgccg ccttccgcag
     1561 tcaagctgac gagcgcattt accatacctt ctgtaatatc ggttaccgtg cagtcgatct
     1621 gattacgtgc actgatcttc atttcgccga ttccgatcat gacctcgctg gcttttacta
     1681 cggcgattgc ctctttgccg acggccagtc caagttcttt tgcggattcc tttgtaatcg
     1741 ttgaggagat cttgtcccca tttgcggttt cgataataac ggaagcgttt acaacgcctt
     1801 ctttaatgga tgcgaccgat cccgcgattt tgttgcgtgc actgattgtc atagtaatgt
     1861 cctcctttat gattgtgatc tgccgttaaa agtttgtgct caacacatat tcggatcgta
     1921 ttgattatat gtaacataac ttataaagga gctgcttgtc aatactgtaa accggaaatt
     1981 tccggatgtt tatgacaata gccgaaaaga agcaaggcag gcgctttcgt gctgaatttc
     2041 aatactgcaa ttgtgacaaa aaacagcggt aaaagtaaga ataattgtaa aaatcagcgt
     2101 attgttacca aataagatat cctttatgat tatcttaaca tttgaatagc aatgtgtttt
     2161 tcacaaagaa gtggcttttt agtcctgcgt atgcggatta aaaagccttt ctattttaag
     2221 aaacggagga aaaaatatga gacaggtagc aatttacgga aaaggcggga tcggaaaatc
     2281 cactacaaca cagaacctta cggcaggcct tgcagacatg ggcaaacata ttatgatcgt
     2341 aggatgcgat ccgaaagcgg attccaca
//
LOCUS       DAOSYE010000331         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35666_length_2364_cov_3.113469, whole genome shotgun sequence.
ACCESSION   DAOSYE010000331 DAOSYE010000000
VERSION     DAOSYE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35666_length_2364_cov_3.113469"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1189)
                     /locus_tag="PLU43_06445"
     CDS             complement(164..1189)
                     /locus_tag="PLU43_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO28086.1"
                     /translation="MTSVRFYGKGAGMVSIKEVADKAGVAISTVSKVLNNYPYVSEET
                     KKKVRAAIHELNYVPNTIAAALSSKQTGRAAILLSLNTQTQAIDEITMQYLSGVLNQA
                     LALNIEIITIFLSMISHMTPEEITRYLQSQGITGLIICGISKDDRYLHQLIESQVFRC
                     VVVDAPITNENTSYVWIDNRRAQYEVAKKTILENTCKRVLYIAGKKNGYVTNERLAGM
                     RQLADELDLKLLVRDGGFSELKARKVTFRYAGNKDAVVCASDLMAIGAMNALTEMDIF
                     RPVCGFDGITLMGYVGKQMNTVRQNFYNIGVNAVTELKRLFDGESGRDIVLPFELVKL
                     YYRDIIC"
     gene            complement(1268..>2364)
                     /locus_tag="PLU43_06450"
     CDS             complement(1268..>2364)
                     /locus_tag="PLU43_06450"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOO28087.1"
                     /translation="GFLQDNLCDLDDYLASETGVEMLKRTPSAFLGSCRFKMPKIEGH
                     EEVYEKVFEIMEKHDIECLFYAGGNDSMDTVKMLSDYAAAHNKPQRFMGVPKTIDNDL
                     PITDHCPGYGSAAKYIATSMKEIIRDNESFGVEKPTVCIVEIMGRNAGWLTAAAALSK
                     GIDCTGPDAVYLPERVFNMDKFMEDVRALAKEKSSVVIAVSEGIHIADGRYVCELGKE
                     VAVDAFGHKQMSGTAVILANKIAEETGLKTRAVEFSTLQRAATHLASLTDINEAFQVG
                     ADAVKAAEAGKTGMMITLDRNGDDPYQCGTSAYDIHAIANVERKVPDEWITDDSKGLN
                     DGYEKYARPLIMGELTPLFVDGLPRHLVRK"
BASE COUNT          620 a          606 c          425 g          713 t
ORIGIN      
        1 aatcaaaaag agttaaaaat aaatggttta gtcatttttt tatttttatt gaaatataac
       61 aaaataacgg gctctttttt tcaaaagaac ccgttatttt gtatactatt aataaaaatt
      121 agtcgttcca actttatatt gttcttttcg tccatttaca gactcagcaa ataatatccc
      181 tgtaatataa ttttacaagc tcaaatggca gtacaatatc ccggcctgac tctccgtcaa
      241 ataatctttt caattccgtg accgcattta ccccaatatt ataaaaattc tgtcgaacgg
      301 tgttcatctg cttcccaaca taccccatca gtgtaattcc atcaaatccg catactggtc
      361 gaaaaatatc catttctgtc agcgcattca tggcaccgat cgccatcaga tcagaagcac
      421 atacaacagc atctttattt cctgcatatc gaaatgtcac ttttcttgct tttaattctg
      481 agaacccgcc atccctgacg agcagtttca gatcaagttc gtctgccagc tgtctcatac
      541 cggcaagccg ctcatttgtt acatagccat tcttctttcc tgcaatatac aggacccttt
      601 tgcatgtatt ttcaagaatc gtctttttgg ccacttcata ttgtgcacgt ctgttgtcga
      661 tccatacata agaagtattt tcatttgtga tcggagcatc cacaactacg catctaaata
      721 cctgggattc gatgagctga tgcagatacc tgtcatcttt ggaaatccca caaatgatca
      781 atccggttat tccctgtgac tggagataac gtgtgatctc ttctggtgtc atatgggaga
      841 tcatggacag aaagatcgta atgatctcga tattcagtgc cagtgcctga ttcagtacgc
      901 ctgataaata ttgcatggtg atctcatcga tcgcctgtgt ctgcgtgttc aggctgagca
      961 gaatagctgc gcggccagtc tgtttggatg aaagtgccgc cgcgatcgta tttggcacat
     1021 aattcagttc atgaatcgct gcacgtactt tcttctttgt ttcctcgctg acatatggat
     1081 aattattcag cacctttgat accgtagata tagcgactcc cgccttatct gcgacctctt
     1141 tgatcgatac cattccggct ccttttccat aaaaacgtac gctcgtcaaa cgcacctata
     1201 acagataagc ccgccgatca taccggcggg cttattttta aaacaaactc atgagatttc
     1261 catccggtta ctttctgaca agatgtcttg gaagaccgtc tacgaataac ggtgtcagct
     1321 ctcccataat cagaggacgg gcatatttct cataaccgtc gttcagtccc ttgctgtcat
     1381 ccgtgatcca ttcgtccgga acttttcttt ccacatttgc aattgcatga atatcatagg
     1441 ctgacgtacc gcactgatac ggatcgtctc cgtttctgtc aagcgtgatc atcatacccg
     1501 tctttccggc ctcagctgct tttaccgcgt cggctccgac ctgaaatgct tcattgatat
     1561 ctgtcaggga agcaagatgt gtagccgctc tctgtaatgt ggaaaactcg acagcacgtg
     1621 tcttaagacc tgtctcctca gcgatcttat ttgcaaggat cactgccgta ccggacatct
     1681 gcttatgtcc aaatgcatct accgcaactt ccttgccgag ttcacatacg taacgtccgt
     1741 ccgcaatatg gataccttcc gataccgcaa tcactacgga agatttctct ttagcaagtg
     1801 ctctgacgtc ttccataaat ttatccatat taaatacacg ctccggcaga taaacagcat
     1861 ccggaccagt acagtcaatt cctttggaaa gagctgcagc agccgtcagc cagcccgcgt
     1921 tacgtcccat gatctcaacg atacataccg tcggcttttc tactccaaaa gactcgttgt
     1981 cacgaatgat ctctttcatg gaagtagcaa tatactttgc tgccgaaccg tacccaggac
     2041 aatgatccgt gattggaaga tcattatcaa tcgtcttcgg cacacccata aaccgctgcg
     2101 gtttattatg tgctgccgcg taatccgaaa gcattttgac cgtatccatt gaatcgttgc
     2161 cgcctgcata gaataaacat tctatatcat gcttttccat aatttcaaat actttttcgt
     2221 aaacctcttc atgcccttcg atcttcggca ttttgaaacg acaggagccc aaaaacgcag
     2281 acggcgttct tttcagcatc tccacacccg tctccgatgc aaggtaatcg tcaagatcgc
     2341 acagattatc ctgtaaaaat ccct
//
LOCUS       DAOSYE010000332         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35915_length_2354_cov_2.801218, whole genome shotgun sequence.
ACCESSION   DAOSYE010000332 DAOSYE010000000
VERSION     DAOSYE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35915_length_2354_cov_2.801218"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..498)
                     /locus_tag="PLU43_06455"
     CDS             complement(4..498)
                     /locus_tag="PLU43_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28088.1"
                     /translation="MKKQILRKYSVLFLSIAIVMSIFPSIASAASGELTGSMTKGSGY
                     TNVDLTSLGDVDWAVWGYSSSGHSTSLSPDESMAGGSGISDLTNIDPDEDDALRGLGY
                     LGINHTFNWSNGTNISIATGANAGLQHNVNATYKTGDGFSFTVPADMAKRRLIVHSRS
                     FRHR"
     gene            complement(650..>2354)
                     /locus_tag="PLU43_06460"
     CDS             complement(650..>2354)
                     /locus_tag="PLU43_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO28089.1"
                     /translation="HIAIMGLYFMKLLKKTGVPLYLSSLITITLMTCYGMMTGLSTST
                     FRALLMFLLGVFSICIKRTYDLLSAAAVSAMLILAENPYYLYDTAFQLSFGAIIGIGV
                     FSPVVEMLLGLHKLDKKIAAANSGSMVKRKMGRGYYTVIRGMVKGIIASLSIQLATLP
                     VIMQNFYQVPIYGTFINLIVVPLMSLLILTGIAAGAAGTIGLPDSIVGGILKISYVIL
                     RLYERLAKTGTGLGGNLWITGKPALWQIVLYVLLLLLIWILYSFRKKRNSGYPAAFAV
                     CAVIAAAICILSVRKKEDFELRAISVGQGDCCFIKGKNTPVIIIDGGSSDEKQAGRYR
                     ILPCLKANAVHTVDYVFLSHLDSDHVNGIFEIMEDESSGIEIKRVIIPKTEAYFLSGE
                     QEKSENYDRLVELAKLRNIPVYLFEAGDTISEGDIRIICLSPAVSDSTLWKNRTLNEN
                     SLVLQITYLPAGFQALFAGDISEDIEKELSDSLTHVHYLKAAHHGSKTATSEWFLEAV
                     DPALTVLSAGRNNSYGHPHAETLERLKNRAFYITYDSGQVTVKIRQGKVTVSTFCADE
                     N"
BASE COUNT          681 a          579 c          424 g          670 t
ORIGIN      
        1 agctcaccta tgccgaaatg aacggctgtg tacaataagc cttctttttg ccatatccgc
       61 cggtaccgta aaggaaaaac cgtcacctgt tttatacgtt gcattaacat tatgctgcag
      121 tcctgcattt gctcccgtcg caatggatat atttgtaccg ttggaccagt tgaatgtatg
      181 gttgattccc aaatatccaa gtccccgcaa agcatcgtcc tcatcaggat caatattcgt
      241 cagatcactg attccgcttc cgcccgccat gctctcgtca ggagaaagag acgtactatg
      301 gcctgaactt gaataacccc atactgccca gtccacatcc ccaagtgaag tcagatccac
      361 attggtataa ccggaacctt ttgtcatcga gccggtaagc tcacctgaag ccgcagacgc
      421 tattgatggg aatatgctca tcactattgc aatgcttaaa aataacacgg aatatttcct
      481 taaaatttgt ttcttcatct actattctcc gcaaaaatca gtattttatc ctttaattaa
      541 ccgaactttc taatcagtta tgtatctacc gtaattccga gtgtgacagt aaaataatta
      601 tatcacagta ttcaattttt ttacaattat tttatgaact aattttcgac taattttcgt
      661 ctgcacaaaa ggtactgacc gttaccttcc cctgtctgat tttcactgta acctgaccac
      721 tgtcataagt aatataaaat gcccggttct tcagccgctc caatgtctcc gcatggggat
      781 gcccatagct gttattacgt ccggccgaca gaaccgtaag tgcgggatcc acagcctcca
      841 gaaaccattc cgaagtggct gttttcgaac catgatgcgc tgccttcaga taatggacgt
      901 gagtcagact gtctgacaat tccttctcaa tgtcttcact gatatctccg gcaaacaaag
      961 cctgaaaacc ggccggcaga tacgtgatct gcagaacaag tgaattttca ttcaacgttc
     1021 tgtttttcca cagcgtactg tctgaaacag ccggtgaaag acagatgatc ctgatatccc
     1081 cttcactgat cgtatcaccg gcttcaaaca gataaaccgg aatattccgc agcttcgcaa
     1141 gttcaacaag acgatcataa ttctctgatt tttcctgctc tccagataaa aaatatgcct
     1201 ctgttttcgg tatgatcacc cgcttgatct ctattccact ggattcatcc tccataattt
     1261 caaaaatacc atttacatga tcactgtcta aatgggaaag aaatacataa tcaaccgtat
     1321 ggaccgcatt cgcttttaaa catggaagga ttctgtatct gcccgcctgc ttttcatcgc
     1381 tgctgcctcc gtctataatg ataacaggtg tattctttcc tttaataaaa cagcagtcac
     1441 cctgcccaac agatatcgca cgtaattcaa agtcttcttt tttccttacg gaaagaatgc
     1501 atatcgcagc tgcaataacc gcacacactg caaaagcggc tggatatccg gaatttcttt
     1561 tcttccggaa agagtacagg atccatatga gcagtaacag cagaacatat aacacgatct
     1621 gccagagggc tggttttcct gttatccata gattcccccc tagccctgtg cctgtttttg
     1681 caagtctttc atatagtctt aatataacat atgatatttt taaaattcca ccaacgatcg
     1741 aatcaggcag tccaatggtc ccggctgctc cggccgcaat gcccgtaaga atcagcaggc
     1801 tcatcagcgg aacgacaatc agatttataa acgtcccata aatcggaacc tgataaaaat
     1861 tttgcatgat gaccggcagt gtagcaagct gtatggaaag acttgcaata attcccttta
     1921 ccattcccct gataactgta taatatcctc ttcccatttt tctttttacc atactgccgg
     1981 aattagcagc tgcaattttt ttgtcaagtt tatgtaaacc aagcaacatt tccactacag
     2041 ggctgaatac cccgattcca attatcgccc caaatgaaag ctgaaaggct gtgtcataca
     2101 aataataggg attctcggcc aaaatcagca ttgcggaaac agccgccgca gaaagcagat
     2161 cgtaggtgcg tttgatacat atactaaata cacccagcaa aaacatgagc agtgcccgaa
     2221 atgtagatgt ggaaagtcca gtcatcattc cgtagcatgt cataagagtg attgttatta
     2281 atgatgacag atagagcggg acacctgttt ttttcaacag cttcataaaa taaagtccca
     2341 taatggcaat atgg
//
LOCUS       DAOSYE010000333         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_35992_length_2351_cov_2.421167, whole genome shotgun sequence.
ACCESSION   DAOSYE010000333 DAOSYE010000000
VERSION     DAOSYE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35992_length_2351_cov_2.421167"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLU43_06465"
     CDS             complement(<1..287)
                     /locus_tag="PLU43_06465"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOO28090.1"
                     /translation="MKKPIMEEKYMKEALKQAKKAQALGEVPIGCVIVYENKIIGRGY
                     NRRNTDKNTLSHAEMTAINKASKKIGDWRLEGCTLYVTLEPCQMCAGACVQA"
     gene            388..1083
                     /locus_tag="PLU43_06470"
     CDS             388..1083
                     /locus_tag="PLU43_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOO28091.1"
                     /translation="MQIHGLQKTTLLDYPGLVAATIFSGGCNLRCPFCHNMNLVKHPE
                     SEPLLSEEEIFSFLNSRKNLLDGICITGGEPTLQKDLIPFIYKIKELGYRVKLDTNGT
                     FPNVLKTLIQNQLIDYVAMDIKSSPDTYAAVCGLKTIDLHPIQESISLLINGTLPYEF
                     RTTVIAQYHNEAIFHEIGNMIRGAENYYLQSFQDSEYVEDHTLCACSKKDLLHYKDLL
                     TQYVRHVELRGVD"
     gene            complement(1096..2178)
                     /locus_tag="PLU43_06475"
     CDS             complement(1096..2178)
                     /locus_tag="PLU43_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28092.1"
                     /translation="MISNQIIQSCIDEVSTISKIDFLVADTLGGTVAYTKQMTSPDKT
                     ILLSFLQSPAETQEVRGELLFKVYEDNQPAYVLVARGGEDTFLIGKIVVSQLQNLVIA
                     YKDRVDRNSFFQNLLLDNLLLVDIYNRAQKLHIEAVQSRCVFVIETKSEKDVNALEMV
                     KELFYTQTGDFVTAVDEKHIIVIKALNSGEGYETLEETARMLVDMLNSEAMTTVRVAY
                     GTIADEIKGVSKSYKEAKMALDVGKIFYIEKAIIAYNTLGIGRLIYQLPVNLCQIFIK
                     EVFGEHIPEEIDDELLMTVNKFFENSLNVSETSRQLYIHRNTLVYRIEKLQKATGLDI
                     RVFDDALTFKIALMVVNYMKYVEEEN"
     gene            complement(2175..2306)
                     /locus_tag="PLU43_06480"
     CDS             complement(2175..2306)
                     /locus_tag="PLU43_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOO28093.1"
                     /translation="MTRGDIWIADFGVSFGSETGFIRPVIIVQNTKAVHGSRERMQK"
BASE COUNT          633 a          572 c          412 g          734 t
ORIGIN      
        1 gcctgtacgc aggcaccggc acacatctga cacggctcaa gcgttacgta aagcgtacag
       61 ccttccagcc tccagtcccc gattttttta ctggctttgt taattgcagt catctccgca
      121 tgggaaagcg tgtttttatc cgtatttctc cgattatatc ccctgccgat aattttattc
      181 tcataaacga tcacacagcc gatcggcact tccccaagcg cctgtgcttt ttttgcctgt
      241 tttagcgctt ccttcatata tttttcttcc atgatcggtt ttttcataaa tacgttttct
      301 ccatttcttg aattccctct catattcgtt tataatttta tttgcggatc atgcacgttc
      361 cattcagtcc gctgaaggag aattcttatg caaatacatg gcttacaaaa gacaacttta
      421 cttgactatc cgggtctggt agctgccacg atcttttccg gcggctgtaa tctgcggtgt
      481 ccgttctgcc acaatatgaa tcttgtaaaa catccggaat ccgaaccgct tctctccgaa
      541 gaggaaattt tttctttttt aaactcacga aaaaatcttt tggacggaat ctgcatcacc
      601 ggcggggaac caacgctgca aaaagatctg attcccttta tctacaaaat aaaagagctt
      661 ggttatcgcg taaaactcga tacaaacggc acctttccaa atgtactgaa aacgctcata
      721 caaaatcagc tcatagacta tgtcgccatg gatattaaat cctctcccga tacgtatgca
      781 gctgtctgcg gtcttaaaac aattgacctt cacccaatac aggaaagtat ttctcttctg
      841 ataaacggaa cgctccctta tgaattccgc acaacagtta ttgcgcagta tcataacgaa
      901 gcgatttttc atgagatcgg aaacatgatc cggggtgctg aaaactatta tctgcagtca
      961 tttcaggatt ctgaatatgt ggaagaccat acgctctgcg cctgttcaaa aaaagatctg
     1021 ctgcattata aagatctgct gacccagtat gtcagacatg tcgaactgcg tggtgttgac
     1081 tgaacagaca gccgtttaat tttcttcctc cacatatttc atataattca cgaccattaa
     1141 tgcgatcttg aacgtcagtg catcatcaaa tacccgaata tccaaaccgg tggctttctg
     1201 cagcttttcg atccgatata cgagtgtatt cctgtgaata taaagctgtc gggatgtttc
     1261 cgatacattc aggctgtttt caaaaaattt attaactgtc atcagaagct catcatcgat
     1321 ctcttccgga atatgctccc caaatacttc cttaataaag atctggcaga gattaaccgg
     1381 aagctgatag atcaatcggc cgatccccag cgtattatat gcaataatcg ctttttctat
     1441 ataaaaaatc tttccgacat ccagcgccat ttttgcttct ttataggatt tggatacccc
     1501 tttgatttca tccgcgatcg taccatatgc tacccgcacg gttgtcatcg cctcggaatt
     1561 cagcatatct acaagcattc ttgctgtttc ctctaatgtt tcgtatcctt ctccgctatt
     1621 taatgcctta ataacaataa tatgtttttc atcgaccgcc gttacaaagt ctccggtctg
     1681 cgtataaaaa agctctttta ccatctcaag tgcattgaca tctttttctg attttgtttc
     1741 gatcacaaat acacagcggg actgtactgc ttcgatatgg agcttctgcg cacggttata
     1801 tatatcaaca agcagcagat tgtccaataa aaggttctga aaaaaactat ttctgtcgac
     1861 cctgtcctta taggcgatca caagattctg cagctggctg acaacgatct ttccgatcag
     1921 aaaggtatct tcccctcctc gtgccaccag gacatatgcc ggctgattat cttcgtatac
     1981 tttaaagagc agctctcctc ttacttcctg ggtttctgcc ggagactgca aaaaactcaa
     2041 taatatcgtt ttatccggag atgtcatctg ttttgtataa gcaacagttc ctccaagcgt
     2101 atcagctact aaaaaatcta ttttcgatat ggtactgacc tcgtcaatac agctctgtat
     2161 aatttgattg gaaatcattt ctgcatcctt tccctgctgc catgcacagc ttttgtattt
     2221 tgaactatta tgacagggcg gataaagcct gtttcactac cgaatgatac accaaaatct
     2281 gcaatccaaa tatcaccacg tgtcattttt agttaactcc cgcaatgatt caagagaagc
     2341 atagggcact g
//
LOCUS       DAOSYE010000334         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36008_length_2350_cov_3.516340, whole genome shotgun sequence.
ACCESSION   DAOSYE010000334 DAOSYE010000000
VERSION     DAOSYE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36008_length_2350_cov_3.516340"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PLU43_06485"
     CDS             <1..1502
                     /locus_tag="PLU43_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO28094.1"
                     /translation="DYGSCVLAMPQGERRRANLNMLLKKAEDYGKTSYKGLFHFVRYI
                     EFLQKYEVDFGEANLLDENDNTVRLMSIHKSKGLEFPICFVAGMSKKINYQDARAGVV
                     LDVDYGIGMDRIDIRQRTKRPTLLKKAAALKLMRESLAEEMRVLYVALTRAEEKLIMT
                     GVVKDVQKTLTEESFLAQAGSFLDFFVYARQNTKTDSCIRIMRNDAAGLTAGEVKEEL
                     SREAVRGELYESLQHKEQGACTEFGNTVRKRLAFVYPYEADPNLAAKVSVSELKKRSM
                     LLQEAADADIPLNEKFLYEDPVIVPYIPKFARTKDTYEAEGALYGTAFHRLLELWDYK
                     NGTDADSIRTYYEGVLEKKRMESELYEAVQMKDLLAFFGTELAARMGRAAQNGMLYRE
                     QPFVIGIGKTLIEPIAAHGEEILHNGLTVPKQEELLLVQGIIDAYFVENGELVVVDYK
                     TDKLGSPAALLKRYHAQLNYYAYALEKMTGLSVKEKTIYSSFQNREIRI"
     gene            1529..2131
                     /locus_tag="PLU43_06490"
     CDS             1529..2131
                     /locus_tag="PLU43_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO28095.1"
                     /translation="MRYRLGIKEISATVFGSVAFIGISMFLLKHDCGKLSEYYFQIRL
                     LLLTVMSALFGPIAGVLIGAGGSELSDMLIFGRASYISVLAFGTYGLFIGKYAKYYGI
                     RDGLFNRHSAAAFNVIQMLSSMIIWGFIVPLLTFLANKQDLLEAEMAGLETTMFNAAA
                     VCLVCTPVFWLIDQGMRLNRKRIDRKKKSKKDDGILHVPG"
     gene            complement(2128..>2350)
                     /locus_tag="PLU43_06495"
     CDS             complement(2128..>2350)
                     /locus_tag="PLU43_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOO28096.1"
                     /translation="YLAGIFGRFIFATISGVVFFGEYAWEGWNPLAYSAVYNAIYIFT
                     EGAITVIILMIPAVHTQLARIREMALEKH"
BASE COUNT          663 a          452 c          653 g          582 t
ORIGIN      
        1 gggattacgg cagctgcgtt ctggcaatgc cgcagggaga acggcgcagg gccaatttaa
       61 atatgctgct gaaaaaagca gaggattatg gaaagacgag ttataaggga ttgtttcatt
      121 tcgtgcgcta tattgaattt ctgcagaaat atgaggttga tttcggagaa gctaatctgc
      181 ttgatgagaa tgacaatacg gtcaggctga tgagtattca taaaagtaaa ggcctggaat
      241 ttcccatatg ctttgtggca ggcatgagca agaagatcaa ttatcaggat gcaagagcag
      301 gcgttgtgct tgatgtggat tatgggatcg gtatggacag gatcgatatc aggcagcgga
      361 caaagagacc gacacttctg aaaaaagctg ctgcactgaa gctgatgcgg gaaagccttg
      421 ccgaagaaat gcgcgtgctt tatgtcgcgc tgacacgtgc ggaagaaaaa ctgatcatga
      481 ccggtgtcgt aaaagacgtg cagaaaacgc ttactgagga gagctttctg gcacaggcag
      541 gatcttttct tgattttttt gtatatgccc ggcagaatac gaaaacagat tcctgcatca
      601 ggatcatgcg aaacgatgcg gcaggactga cggcagggga agtgaaagaa gagctgtcca
      661 gggaagctgt acgcggagaa ctgtatgaga gcctgcagca taaagaacag ggagcgtgta
      721 ctgaatttgg gaatacggtc aggaaacgcc tcgcatttgt ctatccatat gaagcagatc
      781 cgaatctggc tgcaaaggtt tccgtctcag agctgaagaa gcgcagtatg ctgctgcagg
      841 aggctgcaga tgcggatatt ccgttaaatg aaaagttttt atatgaggat ccggtcatcg
      901 taccgtatat tccgaaattt gcacggacaa aagacacgta tgaggcggag ggggcccttt
      961 acggaacagc atttcacaga ctgcttgagc tgtgggatta taaaaatggg acggatgcgg
     1021 acagtattcg tacttactat gaaggtgtgc ttgaaaaaaa acgaatggag tccgagcttt
     1081 atgaagctgt gcagatgaag gatctgcttg cattttttgg aacagagctt gcagcgcgga
     1141 tgggcagggc tgcacaaaac ggcatgctgt accgggaaca gccatttgtg atcggtatcg
     1201 ggaaaactct gatagaaccg atcgctgcac atggagagga gatcctgcac aatggtttga
     1261 ctgtgccaaa acaggaagag ctgctccttg tacagggaat catagatgcg tattttgttg
     1321 aaaacgggga gcttgtggtc gtggattata agacggacaa acttggctcg ccggcggcgc
     1381 tcctgaagcg gtatcatgcc cagctcaatt attatgcata tgcgcttgaa aaaatgacag
     1441 gactttcggt aaaggaaaag acgatctatt cttcttttca gaatcgggag atcaggatct
     1501 gataaatcat attacggaag gaaaacagat gaggtacaga cttgggataa aagaaatttc
     1561 ggcgacagta tttggttcgg ttgcatttat cggaatcagt atgtttttgt taaagcatga
     1621 ctgcggaaag ctgtctgagt attattttca gataagactg ctgctgctga cagtgatgtc
     1681 cgccctgttc ggtccaattg ccggcgtgct gatcggagcc ggcggcagtg aactttccga
     1741 catgcttata tttggacggg catcatatat cagtgtgctt gcgtttggaa cttacgggct
     1801 ctttatcggg aaatatgcga agtattatgg aatcagggac ggccttttca acaggcacag
     1861 tgcggctgca tttaatgtca tacaaatgct gtccagcatg atcatatggg ggtttattgt
     1921 tcctcttctt acgtttcttg caaacaaaca ggatctgctg gaagctgaaa tggcaggact
     1981 ggaaacgact atgtttaacg ccgctgctgt ctgtcttgtc tgtacaccgg tcttttggct
     2041 gatcgatcag ggaatgagac ttaacaggaa aagaattgac cggaaaaaga aaagcaagaa
     2101 ggacgacgga atacttcacg tcccgggtta atgtttttcc agtgccattt ccctgatgcg
     2161 cgcaagctgc gtatgtactg caggaatcat caggatgatg accgtgatcg cgccctccgt
     2221 gaaaatatag atcgcattgt agactgccga gtaagcgagc ggattccagc cttcccatgc
     2281 atattcacca aagaatacaa cgccggagat cgtggcaaaa atgaatcttc caaagatgcc
     2341 ggcgagatat
//
LOCUS       DAOSYE010000335         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36275_length_2340_cov_3.471335, whole genome shotgun sequence.
ACCESSION   DAOSYE010000335 DAOSYE010000000
VERSION     DAOSYE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36275_length_2340_cov_3.471335"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..193)
                     /locus_tag="PLU43_06500"
     CDS             complement(44..193)
                     /locus_tag="PLU43_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28097.1"
                     /translation="MTAVNRKGEPVCEATVVKVMNPESFDHTPVVTVEIPKEYADNVR
                     SIKRL"
     gene            complement(190..537)
                     /locus_tag="PLU43_06505"
     CDS             complement(190..537)
                     /locus_tag="PLU43_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO28098.1"
                     /translation="MALINTGYLEYEELASIQKLPTQERYEKGPVAVIECVQEIPCNP
                     CEAACRFGAISVGDPITNLPALDSSKCTGCAICVSKCPGLAIFVVNKAYGAATATVAF
                     PYNIFRHPCRDRR"
     gene            complement(566..1705)
                     /locus_tag="PLU43_06510"
     CDS             complement(566..1705)
                     /locus_tag="PLU43_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO28099.1"
                     /translation="MIENRDIVVVGAGPAGLAASIEAGRAGADVLLIDLNMKPGGQLF
                     KQIHKFFGSSAHRSGTRGYDIGLQLLKEAEKAGVEIWLQSTVMGVFPGNLIGIEKGTD
                     AGEKELVTLKAKKIIVATGASENVVRFKGWTIPGVMGAGAAQTMINVNHVRPGRKIVM
                     LGSGNVGLIVSYQLMQAGCDVVALIEAAPKIGGYAVHAAKIRRAGVPIYTKHTIVEAI
                     PGEDKTVSKVVIAEVDDKFQPIPGTEETIEADTVTIGAGLKPALDIVKLAKCDLTFNG
                     PFGGFIPLHNEKMETSKEGIYAAGDATGVEEANTALEEGKIAGISAAESLGLISSDKA
                     EAEREEIWKRLTALRMGPFGERRLKEKENVLHEYKEKIGAPSEWN"
     gene            complement(1702..2022)
                     /locus_tag="PLU43_06515"
     CDS             complement(1702..2022)
                     /locus_tag="PLU43_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO28100.1"
                     /translation="MCLRVEDHVILDVDKKGPKVTITVDGKVIEAYEGEPILAALLSS
                     GIRINRYTVKKHEPRGIFCGIGQCSDCSMIVDGQPNVRTCITPVKAGMVIKTQDGLSK
                     GESK"
     gene            complement(2104..>2340)
                     /locus_tag="PLU43_06520"
     CDS             complement(2104..>2340)
                     /locus_tag="PLU43_06520"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO28101.1"
                     /translation="QTKATMENAKAVLEAAGASMADVAKVTIYISDITLIPEMNEVYK
                     TYFSEGDYPARCCTQCVKMVDGCLVEMEFTAVIL"
BASE COUNT          527 a          679 c          440 g          694 t
ORIGIN      
        1 ccatacgcga acagtcttcg tatgagaatc ctttccttgt tatttacagt ctcttgatac
       61 tgcgtacatt atcagcatac tccttcggaa tttccaccgt gacgaccggt gtatggtcaa
      121 agctctccgg gttcatcacc ttcactaccg ttgcttcaca gacaggctcg ccttttctgt
      181 tgacggccgt caccttctgt ccctgcaggg gtgtcggaaa atattataag gaaacgcaac
      241 cgttgccgtt gccgcaccgt atgccttatt tacgacaaag atcgcaagtc ccggacattt
      301 tgaaacgcat attgcacagc cggtgcattt gctgctgtca agcgcaggca gattcgtgat
      361 cggatcaccc acggaaattg ctccaaaacg gcacgctgct tcacacggat tgcatgggat
      421 ctcctgtaca cattcaatca ctgccaccgg acccttttca tagcgctctt gtgtcggaag
      481 cttttgtatg gatgccagtt cttcatattc taaataacct gtattaatta atgccatgtt
      541 ccttcctccg cttttaccgt gccctttaat tccattctga cggagcaccg attttttctt
      601 tatattcatg cagtacattt tctttttcct ttaatctgcg ttcaccaaac ggtcccatac
      661 gaagggctgt cagccgtttc catatttctt ctcgttctgc ctctgcttta tcagaagaaa
      721 tcagccccag actttccgca gcggagatcc cggcgatttt tccttcttcc agtgccgtgt
      781 tggcttcctc aacgccggtt gcatcgccgg ctgcgtaaat gccttccttc gaagtttcca
      841 ttttttcatt atgcagcgga ataaatccgc cgaatggtcc gttaaaggtc agatcacact
      901 ttgcaagctt tacaatatcc aatgccggtt tcagtcctgc accaatcgtt accgtatccg
      961 cttcaattgt ttcttctgtt cccggaatcg gctggaactt atcgtcaacc tctgcaataa
     1021 cgactttgga aacggtctta tcctcacccg gaattgcttc cacaattgta tgctttgtat
     1081 agatcggaac acctgcacgt ctgatctttg ccgcatggac cgcatatccg ccgattttcg
     1141 gtgcggcttc gatcagtgcc accacatcac aacctgcctg catcagctga taggatacga
     1201 tcagaccaac attgccgctc ccaagcatga cgatttttct gcccggtctt acatgattga
     1261 cattgatcat tgtctgagct gctccggctc ccatcacccc cggaatcgtc cagcctttga
     1321 atcgtacgac attctcactt gcaccggtcg ccacaatgat cttctttgct tttagcgtca
     1381 caagctcttt ctctcctgca tccgtcccct tttcaatacc gatcagattg ccgggaaaaa
     1441 cacccatgac cgtactctgg agccatattt ccacaccggc tttttctgct tctttcaaaa
     1501 gctgaagccc gatatcatac cctctcgtac cggaacgatg cgcactggaa ccaaaaaatt
     1561 tgtggatctg cttgaacagc tgtcctcccg gtttcatatt caaatcaatc agcagtacgt
     1621 cggcacctgc ccgtccggcc tcaatagaag cggcaagtcc tgccgggccg gcaccgacca
     1681 caacgatatc cctgttttcg atcattttga ttcccccttt gataatccgt cctgtgtttt
     1741 gatcaccata ccggctttta ccggagtaat acaggttctg acattgggct gtccgtccac
     1801 gatcatcgaa cagtctgagc actgtccgat tccacagaaa attcctctcg gctcatgttt
     1861 cttcaccgta taacggttga ttctgattcc acttgataaa agcgctgcta aaatcggctc
     1921 gccctcatac gcctcgatga ccttaccgtc tacggtaata gttaccttcg gtcccttctt
     1981 atccacgtct aaaataacgt gatcttctac tctcaaacac atacgatagc ctcctgtttt
     2041 atccctatgc atacggcaca tcccggttgc cgatgtgccg tactgaatac tttatttctg
     2101 tatttataaa ataactgcgg tgaattccat ttccacaaga cagccatcca ccatcttaac
     2161 acactgcgta cagcatcttg ccggatagtc tccttctgaa aaataagtct tataaacctc
     2221 attcatttcc ggaatcagag taatatcgga aatgtagatc gtcaccttcg caacatctgc
     2281 catggaggca cccgcagctt ccaatactgc ttttgcattt tccatcgtag cctttgtctg
//
LOCUS       DAOSYE010000336         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36309_length_2339_cov_2.587566, whole genome shotgun sequence.
ACCESSION   DAOSYE010000336 DAOSYE010000000
VERSION     DAOSYE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36309_length_2339_cov_2.587566"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLU43_06525"
     CDS             complement(<1..447)
                     /locus_tag="PLU43_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28102.1"
                     /translation="MNSDQLSKKNTRSKRKCILDGAVQVFTKAGFDAASMDKIAETAG
                     VSKITIYKYFQNKETLFLAVVTEYLKENEAHKPVSYSPSQTIESQLHAFIQAELFRVK
                     DEKQRGMSKLLTSVYLYKTELVKKTMQEHPFFTDFIAWMKMAKQDGK"
     gene            567..1229
                     /locus_tag="PLU43_06530"
     CDS             567..1229
                     /locus_tag="PLU43_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HOO28103.1"
                     /translation="MRKTTNSYRLYELTAGTMYLLCMVTSIAGGLLIEQTFNTGDILQ
                     AVSDHYGTLLLAAVFDVINAAGVIGIAVSFYCILKTKKPAMSAGYICLRSMEAAFCLV
                     VAMIPVVSVSLVKNGGNLSGNQEEWLRMLLFVRTEFWAYLYPIVFIAGAALFYSMLYM
                     TKTVPAYIAIWGLLALAGVAAAVFMPAVKMIPGAGIIANELYLGGYLLIKGVRTDEPA
                     GA"
     gene            complement(1239..>2339)
                     /locus_tag="PLU43_06535"
     CDS             complement(1239..>2339)
                     /locus_tag="PLU43_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28104.1"
                     /translation="LFVAYDVEYHNTKSTDYTNLNQELVDAFDGITFVGAKNALRSIE
                     ELRGSGEFTQAELASVFSAGYAGKRNAVLYAAIENKIDFLLFLDDDEYPMAVTNNHQT
                     SLWGGQQVFLSHIKEIEHADYTNGYHCGYISPIPQVKFNDVLDENSFRLFIEAISNDI
                     INWESIRTLMESGGVTYASTDILTSGKASDVPLVGGCKFISGANLCINIKTPQKTLPF
                     FNPPGARGEDTFLSAMLQDRIVRKIPCYTFHDGFSIYQHLLDGVLPIHLSEITAASPA
                     INTRFLNACIGWVRYKPLLVYITDQENYEPRMRSLKNALTKTLPKMAEYFQNERFFLI
                     STEFDKYSKNVKRHYNQFLLAQRTWERIIGTL"
BASE COUNT          599 a          445 c          554 g          741 t
ORIGIN      
        1 ttttccgtcc tgttttgcca ttttcatcca tgcaataaaa tccgtgaaaa acggatgctc
       61 ctgcatagtc ttttttacaa gctcggtttt atacagataa acggaagtca gaagttttga
      121 catgcccctt tgtttttcat cttttacacg gaataattct gcctggatga atgcgtgcag
      181 ctgggactcg atcgtctgcg atggggaata ggaaacgggt ttatgtgcct cattttcttt
      241 cagatattca gttacgacag ccaggaataa agtttcctta ttttgaaaat atttatagat
      301 cgtgattttg gatacaccgg cagtttctgc aatcttatcc atgctggcag catcaaatcc
      361 tgcttttgta aagacttgga ctgcgccatc cagtatgcat tttcttttag aacgtgtatt
      421 ttttttagat aactgatcag aattcatagg ttcctccatt tgaactatac cgtatagttc
      481 attttttatt gacttaaact gtactatacg gtatagtata aaacgtaact attataaaga
      541 caagagattt ttaaaggaga cagtttatga gaaagacaac aaattcatat cggttgtatg
      601 aactgacagc cggtacgatg tatctgcttt gtatggtaac gagtattgca ggaggactgc
      661 tgattgagca gacttttaac accggagaca ttctgcaggc agtttccgat cattatggaa
      721 cgctgctgct tgcggctgtg tttgatgtaa tcaatgcggc aggtgttata ggaatcgcgg
      781 tttcctttta ctgtatactg aaaacaaaaa agcctgccat gtcggcaggg tatatttgtc
      841 tgcggagtat ggaagcagcc ttctgccttg tggtggcaat gattccggtt gtgtccgtat
      901 cgcttgtgaa aaatggagga aatttatccg gcaatcagga agagtggctt aggatgctgt
      961 tatttgttcg tacggaattt tgggcttatt tgtatccaat cgtttttata gcaggtgcgg
     1021 ctttgttcta tagtatgctg tatatgacca aaacagtgcc tgcctatatt gcgatttggg
     1081 gcttgcttgc tttggctgga gtggctgcgg ctgtatttat gcctgctgtc aaaatgattc
     1141 cgggagccgg gatcattgca aatgagttat atcttggcgg ttatctgctc ataaagggag
     1201 tgaggacaga tgaacccgca ggagcatgag cggtttgttt acagagtgcc gatgattctt
     1261 tcccatgtac gctgcgccaa aagaaactgg ttataatggc gttttacgtt tttggaatat
     1321 ttatcgaatt ccgttgatat aaggaaaaat cgttcattct gaaaatattc cgccatttta
     1381 ggaagtgttt ttgtcagtgc atttttcagg gaacgcattc tcggttcgta attttcctga
     1441 tcggtaatgt aaactaacag cggtttataa cgtacccagc caatacatgc atttaaaaat
     1501 ctcgtattga tcgcaggtga ggcggcggtg atttcggaaa gatggatcgg aagcacgccg
     1561 tcaagcagat gctggtagat cgaaaatccg tcatggaatg tatagcatgg tattttgcga
     1621 acgatccggt cctgcagcat ggcggataaa aaggtgtctt ccccgcgtgc gcccggcgga
     1681 ttaaaaaatg gaagtgtttt ttgcggtgtt tttatgttga tgcagaggtt ggctccggaa
     1741 atgaatttac agccgcccac aaggggaaca tcggaagcct ttccgcttgt taaaatatct
     1801 gttgaagcgt atgtaacgcc gccggattcc atcagtgtcc ggatactttc ccagttgata
     1861 atatcattgc tgatcgcctc gataaacagt cggaaactgt tttcgtccag aacatcattg
     1921 aattttacct gtgggatcgg ggagatatat ccgcagtgat aaccgtttgt ataatccgca
     1981 tgttcgatct cttttatgtg agagaggaaa acctgctgac cgccccaaag acttgtctga
     2041 tggttgtttg ttactgccat cggatattcg tcgtcgtcaa gaaacaggag aaagtcgatc
     2101 ttgttttcaa ttgccgcgta taaaacggca ttgcgttttc ctgcatatcc ggcactaaaa
     2161 acgcttgcca gttcagcttg tgtgaattct ccgctgccgc ggagttcttc aatgcttctc
     2221 aaagcgtttt tagctccgac aaaagtgatg ccgtcaaaag catctacgag ttcctgattt
     2281 aaatttgtat agtcggttga tttggtatta tgatattcca catcataggc aacgaagag
//
LOCUS       DAOSYE010000337         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36345_length_2337_cov_2.863716, whole genome shotgun sequence.
ACCESSION   DAOSYE010000337 DAOSYE010000000
VERSION     DAOSYE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36345_length_2337_cov_2.863716"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLU43_06540"
     CDS             <1..1398
                     /locus_tag="PLU43_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO28105.1"
                     /translation="KCYVCGNRLSEKDFCTNCGADVSVYKRILRISNKLYNDGLEKAN
                     VRDLSGATVSLKQSLKYNKRNIEARNLLGLVYFEMGEAVLALSEWIISKNLESKKNIA
                     DDYIQELQSNPTQLDMINQTIKKYNQALTYCYQESQDLAVIQLKKVLSINQNLISGYQ
                     LLALLYISVEDYEKARRTLLRAIRIDANNTITLRYLKEINTILREKEAHSGSKKKKAQ
                     KEVFSYQSGNDMIIQPVYTKERAGFSSIINIVIGIVIGIAICWFLILPARVEIETDKN
                     DEKFIEVSEELSNEKANSQEIEQTLTAVQKENEDLKAQVEELTGESGLTTVNDYLIQA
                     ALAYINAPDDSETIMDALGSITEDNLNEASQPFISLYTLLHEDAAAKAIAVYVDNGNS
                     AMKSNDYETAIEEYTKAWNLDNTNSDVLMNLAHAYRKAENTDKADELYRLVIADFPDT
                     QNAADAEGYITETDN"
     gene            1434..2261
                     /locus_tag="PLU43_06545"
     CDS             1434..2261
                     /locus_tag="PLU43_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO28106.1"
                     /translation="MGKKNSSFSVKPFIILAAMAVFICFALDTTQETLKSWQFFMVLV
                     SVLLVFFVFRKYLPISDVSVIIGSGMFLKLAYVLYTAVWTRQHDVVDFGTGEGHAGYI
                     EYILQNHALPDFDPRTVWAFFHPPLHHIIAALWMKISLYAGLAYRQAQENIQALTLCY
                     IGAVMLFSYFICRELGLKKWGMRAAMLLISFHPVYIILSGSINNDALSLALTAAALYF
                     AVIWYKNPGIPVIHLLAACIGLAMMAKLSGGMIALAVGALFLLKFITDRTNWKRYLG"
BASE COUNT          654 a          448 c          614 g          621 t
ORIGIN      
        1 aagtgttatg tatgcggaaa tcgattgtca gaaaaggatt tttgcacgaa ctgcggagca
       61 gatgtttcgg tatataaacg gatccttcgg atatcaaaca aactgtataa tgacggtctt
      121 gaaaaggcaa atgtaaggga tctgtccggg gcgaccgtaa gtcttaaaca gagtttgaaa
      181 tataacaaaa gaaacattga ggcgcgaaat ctgctcggac ttgtctattt tgaaatgggc
      241 gaggccgttt tagcgttgag cgagtggatc atcagtaaaa atcttgaaag caaaaaaaat
      301 attgctgacg attatattca ggaactgcag tctaatccga cgcagctgga catgatcaac
      361 cagacgatca agaagtacaa tcaggcattg acctactgct atcaggaaag tcaggatctt
      421 gccgttattc agctgaaaaa agtattatcg atcaaccaga acctgatcag cgggtatcag
      481 cttctggcat tattgtatat ttccgtcgag gattatgaaa aggcaaggcg gactcttttg
      541 cgtgcaatca ggatcgatgc aaataataca attaccctgc gttatctgaa agagatcaat
      601 acaattctgc gggaaaagga agcgcattcc ggttccaaaa agaaaaaggc gcagaaagag
      661 gttttcagct atcagagcgg gaacgatatg atcattcagc cggtgtatac aaaggaaaga
      721 gccgggtttt ccagtattat caatatcgtg atcggtattg tgatcggcat tgccatttgc
      781 tggtttttga tccttccggc cagggtggaa attgagactg ataaaaacga tgagaaattt
      841 atcgaagtaa gcgaggaact gtcaaacgag aaggcgaaca gtcaggagat cgaacagaca
      901 ctgacggcag tacagaagga aaatgaggat ctgaaggcgc aggtcgagga actgaccggc
      961 gaatccggcc tgacgactgt caatgactat ttgatacagg cagcgcttgc atatatcaat
     1021 gccccggatg attcggagac gatcatggat gcgcttggca gtattactga ggataatctg
     1081 aacgaggctt cgcagccgtt tatatcatta tatacactgc tgcatgaaga tgccgcagcg
     1141 aaagcaatcg ctgtttatgt ggataatggg aacagcgcca tgaaatccaa tgattatgaa
     1201 acagcgatcg aagaatacac gaaggcatgg aatctggata ataccaattc cgatgtattg
     1261 atgaatctgg cgcatgcata tcggaaagcg gagaatacgg ataaggccga cgaactgtat
     1321 cgtctggtca tagcggattt tccggataca cagaatgcgg cggatgccga aggatatatt
     1381 accgaaaccg ataactgatc aggtcgtttc aggaaggcac ggataacgga cagatgggaa
     1441 aaaagaactc ttcttttagt gtgaaaccat ttatcatatt ggcagcgatg gcggtcttta
     1501 tctgctttgc actcgatacg acgcaggaga cgctgaaatc atggcagttt ttcatggtgc
     1561 tggtttccgt actgcttgtt ttttttgtat tccgcaaata tctgccgatc agtgatgttt
     1621 ccgtgatcat cgggagcgga atgtttttaa aactggctta tgtgctttat actgccgtat
     1681 ggacgagaca gcatgatgtt gtggatttcg gtacgggaga agggcatgcc ggttatattg
     1741 agtatatttt acaaaatcat gcgctgccgg attttgatcc gcgcactgtc tgggcgtttt
     1801 tccatccgcc gctgcaccat ataatcgctg cactctggat gaagatcagt ttgtatgcag
     1861 gtcttgctta caggcaggca caggagaaca tacaggcgct gacgctttgc tatataggcg
     1921 ctgttatgct gttttcgtat tttatatgca gggagcttgg actgaaaaag tggggaatga
     1981 gagcagccat gcttctgatt tcttttcacc cggtctatat tattttgtcg gggagcatca
     2041 ataatgatgc tctgtcactt gcgctgacgg cggcggccct gtattttgcc gttatatggt
     2101 acaaaaatcc cggtattccg gtgatccatc tgctggcagc ctgtatcggg cttgccatga
     2161 tggcgaagct gtccggcggg atgatcgcgc ttgcggtcgg tgcgctgttt ttgctgaaat
     2221 tcataacaga tcgtacgaat tggaaaagat atttgggcta gatggctgcg tttgcggttg
     2281 ttgtgtttcc gcttggactc tggtggccgg tcagaaataa aatattatac gacatgc
//
LOCUS       DAOSYE010000338         2335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36394_length_2335_cov_3.237719, whole genome shotgun sequence.
ACCESSION   DAOSYE010000338 DAOSYE010000000
VERSION     DAOSYE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36394_length_2335_cov_3.237719"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1107)
                     /locus_tag="PLU43_06550"
     CDS             complement(136..1107)
                     /locus_tag="PLU43_06550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO28107.1"
                     /translation="MNYIVLDLEWNQSTEKFDRECGLPFEIVEIGAVKLNRKMNMEDH
                     FEQIIKPQIYQTIHYMTSKIIHMEKKELDKGRPFPEVIQEFLDWCGKDSIFCIWGTLD
                     LTELQRNMCYYGMDELSKGPLKYYDIQKFFSIAYEDGRIRRALEYAVDYLNIEKDIPF
                     HRAYSDAYYTAKVFKEIDSVNPEVERYVSYDVFMIPKNKREEIKVNFKTYSKYISREF
                     PDKTAAIADRTVNTTKCYLCGRTARKKIKWFSVNGKHYYCLAYCKKHGYLKGKIRMKK
                     AEDGNVYVIKTLKLVPEEVAREIREKQERVRIQRSQKRRSKKGAVAK"
     gene            complement(1218..2039)
                     /locus_tag="PLU43_06555"
     CDS             complement(1218..2039)
                     /locus_tag="PLU43_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28108.1"
                     /translation="MEKRNKQTGRRKAMAERRKKKQFLDILKLAWVEGGQRFSKEQFY
                     SYMEGLDYLQNTGESMDLNKEEILDIFMYIDKTVRIYTAYSIRKMKIETEIINSLQNL
                     YFILEYLFVFHQEKISVDQPLELLYSIIGIEQYNFEKLEFLLRFYEESELLDKICFYL
                     YYYFDFICRAQLLEKFLSKIDQTDHYRLFNIILYQINPAYIKQRDYIQVSECKPKELY
                     YGICYGYYFNDHKIEVYKYLLKLCNIVKNQNKTGDSIDNPVVVFSRLFQDGYFNA"
BASE COUNT          692 a          469 c          316 g          858 t
ORIGIN      
        1 tcttcgaaaa tatttatatt caaaacagca tcgaaggaat ctgaaaagaa agcgttacaa
       61 aaaaagaaac cggacgatct ttcattgatc ttatcaagcc tcaaaaaagc tgctgctcca
      121 taactgactt ctcagttatt ttgcaacagc tcctttttta cttctccttt tttgagaccg
      181 ctgtatcctt acccgttcct gcttctccct gatttctctt gcaacctctt ccggcactaa
      241 ttttaatgtc ttaattacat aaacatttcc atcctcggct tttttcatcc gtattttacc
      301 ttttaaatat ccgtgttttt tacaataagc cagacaataa taatgtttgc cgtttaccga
      361 aaaccacttg attttttttc tggcagttct tccgcacaga taacattttg tggtatttac
      421 cgttctgtcg gcaattgcag ctgtcttatc gggaaattct ctggaaatat attttgaata
      481 tgtcttaaaa ttaactttta tttcttctcg tttgtttttc ggaatcataa aaacatcata
      541 cgaaacatat ctttcaactt ccgggtttac actgtcgatc tccttaaaaa cctttgccgt
      601 atagtaagcg tcactatacg cacgatgaaa aggaatatcc ttttctatat ttaaataatc
      661 aacggcatat tccaatgccc gtctgattct tccatcctca taagcgatac tgaaaaattt
      721 ttggatatca taatatttca gtggtccttt tgataattca tccattccat aataacacat
      781 gtttcgctga agctctgtca aatccagtgt gccccatata caaaaaatgc tgtcttttcc
      841 gcaccagtca agaaattcct gtattacttc aggaaaaggc cggcctttat ccagctcctt
      901 tttttccata tgtataattt ttgatgtcat gtaatggatc gtttgataga tctgcggttt
      961 tatgatttgt tcaaaatgat cttccatatt catttttcta ttgagcttca cagcgcctat
     1021 ttcaacaatt tcaaaaggaa gtccgcattc cctgtcaaat ttttctgtcg attgattcca
     1081 ttccaaatca agtacaatgt aattcatcta tcttctccgg aatttctttt agcagaaaaa
     1141 atatctgctt ttttattata ctcttatatg gaattgtttt caatcaaatc ctgtgagctg
     1201 tcgcggaaga aaggctgtta tgcattaaaa taaccatcct gaaatagtct ggaaaataca
     1261 acgacaggat tatcaatgct atctcctgtt ttattttggt ttttcacaat attgcaaagt
     1321 tttagcaaat atttgtaaac ctcaatttta tgatcattga aatagtatcc ataacaaatt
     1381 ccataatata attcttttgg tttacattcg ctgacctgaa tataatcccg ctgtttgata
     1441 tatgcaggat ttatttgata caaaatgata ttaaaaagac ggtaatgatc cgtttggtca
     1501 atttttgata aaaacttctc cagtaattgt gccctgcata taaaatcaaa ataatagtaa
     1561 agataaaaac agattttatc cagaagttcg ctttcttcat aaaatctcag taaaaattcc
     1621 agcttttcaa aattatattg ttcgatacca ataatcgaat ataataattc cagcggttgg
     1681 tcgacagaaa ttttttcctg atggaaaaca aataaatatt ccaaaataaa atataaattc
     1741 tgcagactgt ttattatttc tgtctctatt ttcatttttc ttatggaata ggccgtatat
     1801 atccgtaccg ttttatcaat atacataaaa atatctaaga tctcttcttt gttgagatcc
     1861 atggattctc cggtattttg taaataatca agcccttcca tataactgta aaactgctct
     1921 tttgaaaatc tctgcccgcc ttcgacccag gccaatttca aaatatccaa aaattgtttc
     1981 ttttttctcc tttcagccat agcttttcgt ctgcccgttt gtttgttcct tttttccata
     2041 taacacttcc cccttcatga aaaatacttg ctttgtgaaa aatattggca tattcttcta
     2101 ggaaaatgtt accatatatt ttttttgatg ataagatgtg aaccttcgac aatttaccgc
     2161 agctattttc tgaatggaat cggagaaagc tcaactttat caactaaata ttccagatcc
     2221 tgttttgtaa gaaaaaactt gatgccatct gccagttctt cctgtcttgc attcggcccg
     2281 acaattattt tttctatcgg ccattgtttc aaactatttt ggcgctcatc gacca
//
LOCUS       DAOSYE010000339         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36468_length_2332_cov_3.314449, whole genome shotgun sequence.
ACCESSION   DAOSYE010000339 DAOSYE010000000
VERSION     DAOSYE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36468_length_2332_cov_3.314449"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLU43_06560"
     CDS             complement(<1..368)
                     /locus_tag="PLU43_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HOO28109.1"
                     /translation="MDKISQSVVKKDHGAKVSGRSIYVGDYSDDRILTGRMLRSKYAR
                     AKILDVIVPKLPDGYYYIDRNDVQGDNRVNIVMDDTPVYAEDTVEYIGEPIGMVCGPD
                     KIKCNQILKEIEVKYEELEP"
     gene            complement(395..1042)
                     /locus_tag="PLU43_06565"
     CDS             complement(395..1042)
                     /locus_tag="PLU43_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO28110.1"
                     /translation="MTILITERAKNNVMCINGLIPAAGLSSRMGEFKPLLPLRGKTVI
                     ENTIDSMLIAGVSQIVLVTGYRAEDVERLVRSRYIENTVICIRNPLYESTDMITSIKT
                     GLHAMPDCDAFFLLPGDMPIVLKDTYLAVYRKMTESGKAVIFPEINGKRKHPPLIRSS
                     FIEKILDFTGQGGLRELWKYHEEEIDGVSVDDTGCRIDLDTYEQYLHCIDRCLEQ"
     gene            complement(1008..>2332)
                     /locus_tag="PLU43_06570"
     CDS             complement(1008..>2332)
                     /locus_tag="PLU43_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO28111.1"
                     /translation="PVKVKYSRQESLNYHVKRHPMEMEFTTACDENGYLTGMKGVIIA
                     DTGAYASLGGPVLHRACTHAAGPYNYQNIDIFGMSVYTNNVVSGAFRGFGVSQSCFAT
                     EMNINLLAEMAGIDPWEMRYRNAIRPGQVLANGQLADPNTNMAACLEAVKDIYYANPY
                     AGIACGFKNSGTGMGKKDIGRIILSVEQGKIHLRTSAACMGQGIGQMVLTEVCHVTGL
                     NPALFVHETADTVRTPDAGTSTASRQTVMTGEAARRAGEKLRLELEAGRSLADLEGME
                     FSGEFSAKTDPLGAVKENPISHISYSYGAQVIILNEEGRITKAVSSFDVGTPVNISSV
                     EGQIEGGMVMGIGFGVTEEFKCIDGYPQSRFGTIGFMRATDAPELEVILCEPPEEDKL
                     PYSLGAKGCGELCMIPTAPACAHAYYRLDGKFRQSLPLDDTYYRKGKK"
BASE COUNT          554 a          648 c          483 g          647 t
ORIGIN      
        1 atcggctcca gctcttcata ctttacttcg atctccttta agatctggtt gcatttgatc
       61 ttgtccggtc cgcagaccat accgatcggc tctccgatat attccaccgt atcttccgca
      121 taaacaggtg tatcgtccat aacgatattt acccggttat ccccctgtac gtcatttctg
      181 tcgatataat aatagccatc cggcagcttc ggaactatga catccaggat ttttgccctc
      241 gcatatttgg atcgcagcat tctgccggtc agtatcctat cgtcggaata gtccccgaca
      301 taaatcgaac ggcctgatac tttggcgcca tgatcctttt ttacaacaga ttgtgatatt
      361 ttatccattt gtctccctcc gataatctaa taatttactg ctccaggcag cggtctatgc
      421 aatgcagata ctgttcatag gtgtccagat ctatccggca tccggtatca tcgacggata
      481 cgccgtcaat ttcctcttca tgatatttcc acaactccct gagtccgccc tgccctgtaa
      541 aatctaagat cttctcaata aaggaagacc ggatcagggg cggatgcttt cttttcccat
      601 tgatctccgg aaatataacg gcttttccgc tctctgtcat tttccgataa acggccagat
      661 acgtatcctt taaaacaatc ggcatatctc ccggcaaaag aaaaaatgcg tcacagtccg
      721 gcattgcatg caggcctgtc tttatggaag taatcatgtc tgtcgattcg tagagaggat
      781 ttctgataca gatcaccgta ttttctatat aacggctccg aacgagcctt tccacatctt
      841 ccgcacggta tcccgtgacc agcacgatct gagatacacc cgcgatcagc atggaatcga
      901 tcgtattttc aattaccgtt tttccgcgca gaggcagaag aggcttgaat tctcccattc
      961 tggaagaaag acctgctgcc gggatcaatc cgttaataca catcacgtta ttttttgccc
     1021 tttctgtaat aagtatcgtc aagcggaagg ctctgccgga atttcccgtc aagacgatag
     1081 tatgcatgcg cacaggccgg cgccgtaggt atcatacaca gctctccgca gccttttgca
     1141 ccgagtgaat acggaagctt gtcttcttcc ggaggctcac ataaaatgac ttccagctcc
     1201 ggtgcatccg tagccctcat aaaaccgatc gttccaaacc ggctctgcgg atagccgtct
     1261 atgcatttga attcttcggt aactccaaag ccgatgccca tgaccatgcc gccttcgatc
     1321 tgtccttcaa cagaagaaat attgaccggc gttccgacat caaacgaaga gaccgctttt
     1381 gtgatccttc cttcttcatt gagtataatg acctgtgctc cataagaata ggaaatatga
     1441 ctgatcgggt tttcctttac agcaccaagc ggatctgttt tggcggaaaa ttcacctgaa
     1501 aattccatgc cctccaaatc agccagagat ctgcctgctt ccagttcaag ccgcagcttt
     1561 tctccggcgc ggcgcgcagc ttcccccgtc ataacggtct gtcttgacgc agtagatgtt
     1621 ccggcatcag gtgttcgtac cgtatctgca gtttcatgta cgaacagtgc aggatttaag
     1681 ccggttacat ggcatacctc ggtcagaacc atctgcccga tcccctgtcc catacaggca
     1741 gcagatgtac gcaggtgaat tttgccctgt tcgaccgaaa ggatgatgcg gccgatatcc
     1801 ttctttccca tgcccgtacc ggaattcttg aacccgcatg caatgccggc ataaggattc
     1861 gcataataaa tatcttttac cgcttccaga catgcggcca tattcgtatt cggatctgca
     1921 agctgtccgt tcgcaagcac ctgtccgggc cggatcgcat tgcggtaccg catttcccac
     1981 ggatcgatgc cggccatctc tgccagcaga ttgatgttca tttccgttgc aaaacagctc
     2041 tgactcactc cgaacccgcg gaatgcaccg gaaacaacat tattcgtata tacagacatc
     2101 ccgaaaatat cgatattctg ataattgtac ggccctgccg catgcgtaca ggctctgtgc
     2161 agaaccggac ccccaagcga tgcataggca cccgtatctg ctatgatgac acctttcatg
     2221 cctgtcagat atccgttttc atcgcaggcc gttgtgaatt ccatttccat cggatgacgc
     2281 tttacatgat aattcaggct ttcctgtctg ctgtatttta cttttacagg ct
//
LOCUS       DAOSYE010000340         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36671_length_2324_cov_2.728955, whole genome shotgun sequence.
ACCESSION   DAOSYE010000340 DAOSYE010000000
VERSION     DAOSYE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36671_length_2324_cov_2.728955"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLU43_06575"
     CDS             <1..344
                     /locus_tag="PLU43_06575"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949067.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO28112.1"
                     /translation="QDKITLTISTNEAGEKAAKLLQTIQEEMLVHAKEHLAAHTYQAK
                     DYDEFKKTVEEKPGFVKAMWCGDEACEDAIKEETGATSRCMPFEQEQIADTCVYCGKP
                     AKAMVYWGKAY"
     gene            368..1561
                     /locus_tag="PLU43_06580"
     CDS             368..1561
                     /locus_tag="PLU43_06580"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOO28113.1"
                     /translation="MNVLVINCGSSSLKYQLIDSDTEEVLAKGLCERIGIDGGMLTHQ
                     KKDGDKIKTQAEMPNHTVAVKLVIESLTDKEHGVISSLDEIDAVGHRVVHGGEKFAEA
                     TLLTADVMKAIEDCNDLAPLHNPANLIGIRSCMEIMPNVPMSAVFDTAFHQTMPKKAY
                     LYGIPYEYYKKYKIRRYGFHGTSHEFVSKRAAKILGKDYNDFKVIVCHLGNGASISAV
                     KNGNCVDTSMGLTPLEGLIMGTRSGDLDPAILEFLANKENLTLSEVTAILNKKSGVLG
                     LSEISSDFRDLGEAAANGNEQAAVTLETYAYRVAKYIGAYAAALGGTDAIVFTAGVGE
                     NNIEARQMIGDYLGYLGTGVDKEKNQVRGEEAILSADNAKVKVMVVPTNEELAIARET
                     VRLLK"
     gene            1584..>2324
                     /locus_tag="PLU43_06585"
     CDS             1584..>2324
                     /locus_tag="PLU43_06585"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO28114.1"
                     /translation="MQIRLPDKVKSIIRTLQDEGYEAYAVGGCVRDSVLGRIPEDWDI
                     TTNAHPLQIKALFRRTVDTGIQHGTVTVMIGKESFEVTTYRIDGEYEDSRHPKEVRFT
                     KTLAEDLRRRDFTINAMAYNEETGIVDVFGGLSDLEKGIIRAVGNAEERFSEDALRIL
                     RAIRFCAQLNYSIEEKTEAAVKKLAKTLANISAERIRTELLKLLLSQSPEKLSIAYET
                     GVIAVILPEFDHMMRTDQNHPHHCFNVGE"
BASE COUNT          730 a          443 c          636 g          515 t
ORIGIN      
        1 cgcaggataa aataactctc actattagta ctaatgaagc cggagaaaaa gcggctaagc
       61 tgctgcagac catacaggag gaaatgcttg tacacgcaaa ggagcatctt gcggcacata
      121 cgtatcaggc aaaggattat gacgaattca aaaagaccgt agaagaaaag ccgggctttg
      181 tgaaggcgat gtggtgcggc gacgaggcat gtgaagatgc gatcaaggag gagacaggag
      241 caacatcccg ctgcatgccg tttgaacagg agcaaattgc agatacgtgt gtttactgtg
      301 gaaaacctgc caaagcaatg gtatactggg ggaaagcata ttaacggaaa aggagaagaa
      361 tcaaattatg aatgttttag ttattaactg cggaagctct tcgctaaaat atcagctgat
      421 tgactccgat acagaggaag tgcttgcaaa aggactttgt gagaggatcg gaatcgacgg
      481 cggcatgctg acacatcaga aaaaggacgg ggacaagatc aaaacacagg cagagatgcc
      541 gaatcatacg gtagctgtca aactggtgat cgaaagcctg acagataagg aacatggtgt
      601 gatctcatcc ctagacgaga tcgacgctgt cggacaccgg gtcgtgcacg gcggtgaaaa
      661 gtttgcggaa gcaacactgc ttacagcaga tgttatgaag gcgatcgagg actgcaatga
      721 ccttgcgccg cttcataatc cggcaaatct gatcggcatt cgttcctgca tggaaattat
      781 gccaaatgta ccgatgagcg ctgtatttga tacggctttt catcaaacga tgccgaaaaa
      841 ggcatatctt tacggaattc catatgaata ttataaaaaa tacaagatcc gccgttacgg
      901 ttttcacgga acaagccatg aatttgtttc aaagcgggcc gccaaaatac tcggaaaaga
      961 ctacaatgat tttaaagtga tcgtatgcca tttgggaaat ggtgcaagca tcagtgcagt
     1021 caaaaacgga aactgtgttg atacgagcat gggactgacc ccattggaag ggctgatcat
     1081 gggaacaaga agcggagatc tggatccggc tattcttgaa tttcttgcga acaaggaaaa
     1141 tctgacgctt tctgaagtga cggctattct gaataaaaaa tcgggagtgc ttggcctgtc
     1201 cgaaatttca agtgatttca gggatttggg agaagcggca gcaaacggaa atgaacaggc
     1261 agctgttacg cttgagacct atgcataccg tgtagcaaaa tatatcggag cctatgcggc
     1321 tgcactgggc ggtactgatg ccattgtgtt tacggcaggc gtcggggaga acaacattga
     1381 agcaagacag atgatcggag attatctcgg atatcttgga acaggtgttg ataaagagaa
     1441 gaaccaagta agaggagagg aagcgatcct ttctgcagat aatgcaaagg tgaaggtgat
     1501 ggtcgttccg acaaatgagg aactggcgat tgcaagagaa accgtcaggc ttttaaaatg
     1561 acaaaagaaa tgagggatcc cgtatgcaga taagacttcc ggacaaggtg aaaagcatca
     1621 tcagaacatt acaggatgaa ggctatgagg cttatgccgt cggcggctgt gtacgggatt
     1681 ctgttttagg aaggatcccg gaggactggg atattacgac aaatgcccat ccgctgcaga
     1741 tcaaggcgtt atttcgaaga acggtagata ccggtatcca gcacggaacc gttacagtta
     1801 tgatcggaaa ggaaagcttt gaggtgacga cctatcggat cgacggagag tatgaggaca
     1861 gcagacatcc gaaggaagtc aggtttacaa agacacttgc tgaagatcta aggcgcaggg
     1921 attttacgat caatgcaatg gcatataatg aagaaacggg aatcgtggat gtatttggag
     1981 gcctgtccga tttagaaaag ggaattatcc gtgcggtcgg aaatgctgag gagcgttttt
     2041 cagaggatgc gctgcggatc cttcgggcga tccgtttttg tgcacagctg aactattcga
     2101 tcgaggaaaa aacagaggca gcagtcaaaa aactggccaa aacacttgca aacatcagcg
     2161 cggaaaggat ccgcaccgaa cttttaaagc ttcttttatc gcagtcgccg gaaaaactca
     2221 gtattgctta tgaaacggga gtaatagcag tgatccttcc ggaatttgat catatgatgc
     2281 ggacagatca gaatcatccg caccattgtt ttaatgttgg agag
//
LOCUS       DAOSYE010000341         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36811_length_2318_cov_2.857711, whole genome shotgun sequence.
ACCESSION   DAOSYE010000341 DAOSYE010000000
VERSION     DAOSYE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36811_length_2318_cov_2.857711"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLU43_06590"
     CDS             complement(<1..1192)
                     /locus_tag="PLU43_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOO28115.1"
                     /translation="MGKKNGYFQLLMKSDGAYVRLFPQKEGGEPISLDECKEYLALKG
                     YKPDAVVLNKTLMRLEQPEDVKIDDKANSAESEMLVLKVSENKMKASIRFYPPSSGGA
                     VLSGEAILSDIRQKGIQFGIREDVISSYLADRCYCMDYLIAEGKLPRQGTDAQIEYLF
                     NTNPNTKPTLNSDGTVDFFNLNIISKCTVGQVLARLIREDRGDDGQNVFGERVSPRDV
                     RRLSLKYGRNIALSDDDLSISSKINGHVSLVDDKVFVTDVYEVNDVDTSTGNIDYKGN
                     VLVLGNVKAGFSVISEGNIEVRGVVEGALLDAGGDIIIIRGMNGMGRGKLRAGGNIVA
                     KFIENADVSAGGYVQTEALLHSKVSAKGDIEVNGKKGFIAGGVIRSRGTVSARVVGST
                     MGVDT"
     gene            complement(1230..1997)
                     /locus_tag="PLU43_06595"
     CDS             complement(1230..1997)
                     /locus_tag="PLU43_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOO28116.1"
                     /translation="MEETARLKLWEDYAKSPTPELREKIILEYAPLVKVVAGRLSMYL
                     GYNVEYDDLVSYGIFGLIDAIDKFDAGKAVKFETYASLRIRGAILDQIRKMDWIPRTV
                     RQRQKKIAAVIKEIELMTGKGATDDKIAEALGISESEYDEWQSQMKVTNLISLDEFVE
                     QGSDVADNRGISSKIECPEEVVEKEELKKILGEALEQLTEKERKVILLYYYEELTLKE
                     ISNILEVSESRISQLHTKGLQKMKTKMGKYMGILLDN"
     gene            complement(2049..>2318)
                     /locus_tag="PLU43_06600"
     CDS             complement(2049..>2318)
                     /locus_tag="PLU43_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28117.1"
                     /translation="PALIMLLAGAVACIVTYLNHYDLKDMLITLIWVLLIFLVIGFGI
                     KRIFDSFHMPDENAVDDEGEVVEKQGAQEAESEEESKAKNMKNES"
BASE COUNT          592 a          658 c          348 g          720 t
ORIGIN      
        1 ccgtatcaac gcccatcgtc gaaccgacca cgcgggcact gaccgtaccg cgggaacgaa
       61 taacgcctcc tgcaataaat cccttcttac cgtttacttc aatatcccct ttggctgaaa
      121 ccttgctgtg caggagtgct tctgtctgca cataaccacc ggcagacaca tccgcatttt
      181 cgataaattt tgcaacgatg ttcccgccgg cacgcaattt gccgcgcccc ataccattca
      241 tgccccgaat gataataata tctcctccag catccaaaag ggctccctca acgacacccc
      301 tgacttcaat attgccttcc gatataacac tgaaaccggc cttcacattt ccaaggacca
      361 atacattccc tttataatca atattacccg tagaagtatc aacatcattt acttcataaa
      421 catccgttac gaaaacctta tcatccacaa gtgagacatg accgtttatc ttggaactga
      481 tcgacagatc atcatcactc agtgcaatat tccgcccata ttttaatgaa agccgcctga
      541 catctcttgg gctgacacgc tctccgaaca cgttctgtcc gtcatctccc cgatcttccc
      601 tgatcagtct tgcaagcacc tgtcctaccg tacatttact gataatattc aggttgaaaa
      661 aatcgaccgt tccgtcactg ttcaaagttg gttttgtatt tggatttgta ttgaacaaat
      721 actcgatttg cgcatccgtt ccctgtctcg gcagttttcc ttccgcaatt agataatcca
      781 tacaataaca tcggtcggcc agatagctgg aaatcacgtc ttccctgatt ccaaactgga
      841 tccccttttg tctgatatca gataaaatag cttctcccga taaaaccgca ccgccgctgg
      901 aaggaggata aaatctgata ctcgccttca ttttattttc tgacacttta agtacaagca
      961 tttccgattc cgcactgttt gctttatcgt cgatttttac atcttccggc tgttccagcc
     1021 gcatcagcgt cttattgagt acgactgcat ccggcttata gccttttaat gccagatatt
     1081 ccttacactc atccaaagaa atcggctcac cgccttcttt ttgcggaaac aatctgacat
     1141 acgccccgtc acttttcatc aataattgaa aatatccgtt ttttttaccc atgtttatcc
     1201 cccaatatga ttggctatac caaacgtgtt caattgtcta ataaaatacc catatatttc
     1261 cccattttcg ttttcatctt ctgcagcccc ttcgtgtgaa gctgcgagat ccttgattcg
     1321 gaaacctcca gaatattact gatttctttt aatgtcagtt cttcataata atacagtaag
     1381 attacttttc tttctttttc cgtaagctgt tccaatgcct ctccaagtat tttcttcagc
     1441 tcctcttttt caacaacctc ttccggacat tcgatcttgg aggaaattcc cctgttatcc
     1501 gctacatcac tcccctgctc aacaaattcg tccaacgata ttaaatttgt cactttcatc
     1561 tgtgactgcc actcatcgta ctcactttcg gaaatgccaa gcgcctcggc tattttatcg
     1621 tcagttgcgc ctttccctgt catcaattca atctccttga taactgccgc aatttttttc
     1681 tgacgctgac gaactgttct tggaatccag tccatcttcc tgatttgatc taaaatggcg
     1741 cctctgattc gcagacttgc ataagtttca aatttgaccg ccttccctgc gtcaaattta
     1801 tcgattgcat caatcaatcc aaaaatacca tagctgacaa gatcatcata ttccacatta
     1861 taaccaagat acatggataa acggcccgct acgaccttta caagcggcgc atattccaat
     1921 atgatctttt cccgcaattc cggtgtcgga ctttttgcat aatcctccca aagctttaat
     1981 cttgcagttt cttccatctt cccctccaac tgcgtcacct tacggcaact tgtttattcc
     2041 ccctcatatc agctctcatt cttcatattt tttgccttgg actcttcttc cgattcagcc
     2101 tcctgcgctc cctgcttttc gacgacttcc ccttcatcat cgaccgcatt ctcatcaggc
     2161 atatgaaaag aatcaaaaat tctttttatc ccgaatccta tgacaagaaa aatcagcaat
     2221 acccaaatca acgttatcag catgtctttt aaatcataat gatttaaata tgtcacgata
     2281 caggctaccg ctccggcaag cagcatgatc agtgccgg
//
LOCUS       DAOSYE010000342         2315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_36882_length_2315_cov_4.012389, whole genome shotgun sequence.
ACCESSION   DAOSYE010000342 DAOSYE010000000
VERSION     DAOSYE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36882_length_2315_cov_4.012389"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PLU43_06605"
     CDS             complement(<1..1279)
                     /locus_tag="PLU43_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HOO28118.1"
                     /translation="MSKYIKLKELAANLGLKKGDNVLVTSDVKQLLYSLMEHGDETDL
                     NVLIDGIINVIGEEATLVFPTFNWSFCKGQAFDYHKTPCKTGSLGKLALQRDDFKRTK
                     HPIYSFAVWGKGKDELCALDNKSSFGKDSPFTYMTQHRFKNLLIDKDCQHSFVYVHYV
                     EEQEGPVAYRYLKDFRAAYTDGNGVTREAVYSMNVRDLDLDVDILIYPFEPDFEAAGV
                     MRRFYVNDMEYKLIDLAGAYPIIAEDVRHNRSRKLCSYIGQNDTAYLEEGKGMFSLAK
                     EIFPICRSITGNGVRETFRILKKYYPDIELTEVPTGTHVFDWIVPREWNITEAYLEDS
                     FGNKIVDFKENNLHVLGYSIPVDTVLSLEELEPHLNTLQEQPELIPYTTSYYKERWGF
                     CMSQKQKETLKPGNYHAVIRSSLTEGSLTYGEIL"
     gene            1604..>2315
                     /locus_tag="PLU43_06610"
     CDS             1604..>2315
                     /locus_tag="PLU43_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HOO28119.1"
                     /translation="MLQLANTSVLYKQLHKKTVYTTEALALSISTYVSSYIESVYNES
                     LLIFRKRDVLSGNKEGQMILEDAVSQFSGLSLIYVQDLDGMQTIRSNGKLANRSDRWW
                     YLKMMEDPSDFVSDAYLSVYNNELVTSIFLPMYDEQDKMIGIYGADLTLNPVRNAIGQ
                     YWDKDISYIIMDSKGTVLAGSDYEQQEFINYMDQTKQTIVLNENGEFSLDDNGQILTT
                     TESIALSDSMQKIIRNALD"
BASE COUNT          649 a          543 c          440 g          683 t
ORIGIN      
        1 tcagaatctc tccatacgtc agactgcctt ccgttagtga agaacgaatg accgcgtggt
       61 aatttcccgg ctttaacgtt tctttctgct tctgcgacat acagaagccc caccgctctt
      121 tataatagga agtcgtatac ggaattaatt ccggctgctc ctgcagcgta tttaaatgcg
      181 gttccagttc ttcaagggaa agcacggtat cgaccggaat agaatatcca agtacgtgca
      241 gattattttc cttaaaatcc acgattttat taccaaaact gtcttccaga taagcctccg
      301 taatattcca ttccctcgga acgatccagt caaacacatg cgttccggtc ggtacttcgg
      361 tcaattcgat atccggataa tattttttta aaatacggaa cgtttcccgc actccgttgc
      421 cggtgatgct gcggcagatc gggaagatct cctttgcaag ggaaaacatg ccctttcctt
      481 cctcaagata tgcggtatca ttctgcccga tataggaaca cagcttccgg ctgcggttat
      541 gcctgacatc ctcggcaatg atcggatacg cacctgccag atcgatcagt ttatattcca
      601 tatcattcac ataaaagcgt ctcatcacgc cggctgcttc aaaatccggc tcaaacggat
      661 agatcagaat atctacgtca aggtcaagat cccggacatt catggaatag accgcttccc
      721 ttgtaacacc attcccatcc gtataagccg ccctgaaatc ttttagatag cggtatgcaa
      781 caggcccttc ctgttcctcc acataatgta catatacaaa agagtgctgg cagtctttat
      841 cgatcagtaa atttttaaac cgatgctgtg tcatataggt aaacggcgaa tcttttccga
      901 atgaactttt attgtcaagc gcgcacagtt cgtctttccc ttttccccat acggcaaaag
      961 aatagatcgg atgcttcgtt cttttaaagt catcccgctg caatgcaagc tttcctaaag
     1021 aaccggtctt gcacggcgtt ttatgataat cgaatgcctg ccctttgcag aacgaccagt
     1081 taaatgtcgg aaatacgagt gttgcctcct cgccgatcac attgatgatc ccatcgatca
     1141 gaacattcag atcggtttcg tccccgtgtt ccatcagact gtacagcagc tgcttgacat
     1201 cggaagtgac cagaacatta tcgccctttt tcaaaccaag gtttgccgca agctctttca
     1261 gcttaatata tttgctcata ctatagtttg tatcctctcc gtacaaaatg cagaccttcc
     1321 atgaaaattc acggcaggct gtttctcaat acattatatc attatatccc gatatcctct
     1381 ttttttcaat aaattcatac ccctggttct aaaagtcagg ccgggtccga tttccttgac
     1441 agtttattca taatgcttta gtatgaaatc agaaactttt tttgaccctg ttattgtacg
     1501 tatatacgtt ggttggttaa aaaatatgaa aaattcgacc tttcattcga tacgatttca
     1561 aatcattatt gccgttactt tactaataag tatccctttt attgtgctgc aattagcaaa
     1621 tacatccgta ctttacaaac aactccacaa aaaaaccgta tacacgacag aagcgcttgc
     1681 attgtccata tcgacctatg tcagcagtta tatcgaaagt gtatataatg aatctttact
     1741 tattttcaga aagagagacg tcctttcagg caataaagaa ggccagatga ttttggaaga
     1801 cgccgtcagc caattctccg gccttagttt aatttatgtg caggatcttg acggcatgca
     1861 gacgatccgc tcaaacggaa aacttgcaaa ccgttccgat cggtggtggt acttgaaaat
     1921 gatggaagat ccatctgatt ttgtcagtga tgcttatttg tccgtatata ataatgagct
     1981 ggtcacttct atttttttac ccatgtatga tgaacaggat aaaatgatcg gtatctacgg
     2041 tgcggatctg acactcaatc cggttcgaaa tgcgatcggc caatactggg acaaagacat
     2101 ctcatacatt attatggaca gcaaagggac tgtacttgca ggctctgatt atgaacagca
     2161 ggaatttatc aattatatgg atcagacaaa acaaacgatc gtcctcaatg aaaatgggga
     2221 gttttctctg gatgacaacg ggcagatact tactactacg gaatcgatcg cgttatctga
     2281 ttccatgcag aaaatcatcc gcaatgcact tgatt
//
LOCUS       DAOSYE010000343         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37009_length_2310_cov_2.813304, whole genome shotgun sequence.
ACCESSION   DAOSYE010000343 DAOSYE010000000
VERSION     DAOSYE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37009_length_2310_cov_2.813304"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLU43_06615"
     CDS             complement(<1..826)
                     /locus_tag="PLU43_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO28120.1"
                     /translation="MKKKLLSALLSAAMVAGLLTGCGQTAETPAADDTAAEASAEEAS
                     TDDAAEETAVEETESTQNMADADIDVSALKGTKIAVYTHGGNRVLGEEKKDEDGNTYR
                     DESSAYLTQLAEKFTEETGIEVELNVVTNEDEIEPLFKVQDASVDVFTCPNWSLAQWE
                     EYTEPYCTVEEASEIYGADYAGSMPNNGSEIFSIMPAKAYNQCVVYNEEVIKAAGYDE
                     IPATLDEFNEMCQAIKDMGVTPISLHRVENWPLATVQDFAGYVVGDANVLAECLKEE"
     gene            1167..2120
                     /locus_tag="PLU43_06620"
     CDS             1167..2120
                     /locus_tag="PLU43_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28121.1"
                     /translation="MGQPKKLLTTEELGEDLVISSRTSKFYPVSGYHSVPDVKGNLLY
                     LSLYGEINAVFPFCYDIKESDSFWIIYTKEGKGKLTAANNSVFLTENTVLFFSGAYAH
                     TLELYDSTKWDFHVLNLNGHALPFYYRTFLTAAPTGSKPELCLSDNLLPVDSAGSIPS
                     LFKQLSALNGTVQKNGGFNHPFTVSLLLTELLTVLIAEKGKNHTNHADIPSYLRTLKN
                     DLDHQYMEPCSLKLLEEKYKICKYTIVHDFSEHFGTSPIRYLIGRRICAAREQLTGTD
                     LPINRIASSVGIENMNHFIYLFKKETGMTPSAYRKNMKGAP"
BASE COUNT          616 a          548 c          403 g          743 t
ORIGIN      
        1 tctcttcttt taagcactca gcaagtacat tggcatcgcc tacaacatac cctgcaaaat
       61 cctgtactgt cgccagcggc cagttttcta ccctgtgaag ggagatcggt gtaacgccca
      121 tatccttgat tgcctgacac atttcattga attcatcaag tgtcgccgga atttcatcat
      181 agcctgctgc cttgatcact tcttcgttat aaacaacaca ctgattataa gcctttgccg
      241 gcataatact gaaaatttcg cttccgttgt tcggcatgga acccgcgtag tcggcaccgt
      301 atatttcaga cgcttcctct accgtacaat atggctccgt atattcttcc cactgggcaa
      361 gactccagtt cgggcatgtg aatacatcaa cacttgcatc ctgcacttta aagagcggct
      421 cgatctcatc ttcattcgtt actacattca attctacttc aatacctgtt tcttccgtaa
      481 atttctcggc aagctgtgta agatatgctg atgattcgtc acggtacgta tttccgtctt
      541 cgtccttctt ctcttctcct aaaactctgt ttccgccatg tgtgtaaacg gcaatttttg
      601 tccctttcag cgcagatacg tctatatccg catcagccat attctgtgtg ctttccgtct
      661 cttcgacagc agtctcctct gccgcatcat ccgtggatgc ctcttctgcg gaagcttctg
      721 ctgcggtatc atctgccgcc ggtgtttccg ctgtctgtcc gcatcctgtc aataacccgg
      781 ctaccatggc tgctgataac agtgcactca ataacttctt tttcatcttt ttccctccat
      841 taaattcgtt tgttgtttta gctaattaat tttagttaat taacttaatt tatttataca
      901 ctaaaatatc tgattaatca tcaccctatt ttgtgtttaa tattgatatc ttgtttttat
      961 tgtatttttt gtatatatta ctatatttca ctgatcattt ttgtatactt tgtcttatat
     1021 gtttttaatt aattaagttt aatattgatt aatattatta attgaatgat tttcattaat
     1081 ttatgggttt ttattatcct taaaataagt tatactgatg ttaattaaca aaacgaatca
     1141 ctttagcaaa cgcggagata aaacatatgg gtcagccaaa aaaactgctt acaacagaag
     1201 aactcggtga agatcttgtc atttcctccc gaacctcaaa attttacccg gtatccggat
     1261 accacagtgt tccggatgtg aaaggtaatc tgctttatct ttctctatac ggagaaatca
     1321 atgctgtttt tcccttctgc tatgatataa aggaatcgga ttcgttctgg atcatttata
     1381 caaaagaagg aaaaggaaaa ttaacggccg caaacaactc tgttttcctt acggaaaata
     1441 ctgtcctgtt tttttccggt gcttacgcac atacgctgga actttacgat tccacaaaat
     1501 gggacttcca cgtgctgaat ttaaacggac atgcattgcc gttctattat cgcactttcc
     1561 tgactgcagc cccaaccggt tcaaagccgg aattgtgctt atctgataat ctccttcccg
     1621 tcgactctgc cggcagcatt ccctcactgt ttaaacagct ttcagcctta aacggcacag
     1681 tccaaaaaaa tggagggttt aatcacccat ttaccgtatc gctccttctt acggagcttc
     1741 ttacagtact gattgcggaa aaagggaaaa atcatacaaa ccatgcggat attccttcct
     1801 atcttcgtac gctgaaaaac gatctggatc atcagtacat ggaaccatgc agcctgaaat
     1861 tactggagga aaaatataag atatgcaaat acacaatcgt tcatgatttt tcagagcatt
     1921 ttggcacttc accgatccgc tatctgatcg gccgccggat ctgtgccgcc agagaacagc
     1981 tgaccggtac agacctgcca atcaaccgga tcgcttcttc tgtcggtatt gaaaatatga
     2041 accatttcat ctatctgttc aaaaaagaaa ccggcatgac accatcggca taccggaaaa
     2101 acatgaaagg agccccctga gactgactgg cagcctgcag gaggcatcat gaaggagtat
     2161 atttcaaaat gaaatatcgt aatttattgg cttcgtccca tttacgaagc agtactatcg
     2221 atactgccga ccgaacgtcc ttccagaata tgacctggaa tgacgatttt ttcaatcagc
     2281 gtcgtctttg gattttcaat caatttaatc
//
LOCUS       DAOSYE010000344         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37028_length_2309_cov_3.598935, whole genome shotgun sequence.
ACCESSION   DAOSYE010000344 DAOSYE010000000
VERSION     DAOSYE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37028_length_2309_cov_3.598935"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLU43_06625"
     CDS             complement(<1..684)
                     /locus_tag="PLU43_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28122.1"
                     /translation="MQYQAAKYLSRGGRIKETATGEFYIVERIEDNRQSILIYGREER
                     TKKPRCFDHRKVEKASLILEMRKMIADKSLYDWKKDVPALYEMLQYKEQLWINPDKED
                     PVPEVAAGFGKKDMEEAAKRMVRFAPYIEKVFPETAPMRGRIRSELIEIPAMLENLKE
                     TDSENIKGKLYLKCDDQLPVAGSVKARGGIYEVLYHAEALALENGLLKENDDYSVLAD
                     ERFRKFFSNY"
     gene            complement(698..1954)
                     /locus_tag="PLU43_06630"
     CDS             complement(698..1954)
                     /locus_tag="PLU43_06630"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOO28123.1"
                     /translation="MHTIYFFSGTGNSMYAAQELAKYVHAAVKPMLKEENRCLEGVIG
                     IVFPVYVNSLPKPVEAFIKRMEFRQVTYLYCIMTHAGMPGKPEGYLNMLLHRKNSRLD
                     EFFSLKMISNTPKGIAPKFLMKMNWETEITKDKVDKMVQNTNEAIRQIAEAVNNRLAV
                     FKEEVENGKRKDSMMNQLLWKMSASPKLVFYSDDTCNGCGICEKVCLSGRIRVDKKPV
                     WTKEECYYCYACFNYCPKQAISVKYYEKKQGRYHFPTIDAEQIAGQKVKSLRFFDTDG
                     KNEEFVYLCGKLDENLDEIVGGRFQRQQYVKYNTLEKIQDVIVVYDKERPIGCGAFRF
                     YDERTAEIKRLYLLPEYRGRGIAGELLKRLEANAKNKGFSTVVLETGEPLTEAMALYK
                     RAGYQVVPNYGAYADMPDSVCMSKKL"
     gene            complement(2021..>2309)
                     /locus_tag="PLU43_06635"
     CDS             complement(2021..>2309)
                     /locus_tag="PLU43_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28124.1"
                     /translation="KPARKETKWSEAVVAPFHLHMERQEDYTLEYKMSREQFIRFMML
                     QSNVTVQIEEKGRSRNEVCQWFEKTTDSFWQKGEHKLYFTGYSWYITKFAE"
BASE COUNT          571 a          605 c          342 g          791 t
ORIGIN      
        1 ataatttgaa aaaaatttcc gaaaacgttc gtctgccaaa acagaatagt cgtcgttttc
       61 ttttaacagc ccgttctcaa gagcaagggc ttccgcatgg tacaaaactt cataaattcc
      121 gcctcttgct ttgacagagc ctgcgaccgg cagctgatca tcacatttca gataaagctt
      181 tcccttaata ttttcgctgt cagtttcttt taagttctca agcattgccg gtatttcaat
      241 cagctccgat cttattctgc ctctcatcgg ggccgtttcc ggaaatacct tttcaatata
      301 cggtgcaaat cgcaccatcc gtttagctgc ctcttccata tcttttttac caaacccggc
      361 agcgacctcc ggcaccggat cttccttatc cggatttatc caaagctgct ctttatactg
      421 cagcatttca tacaaggccg gcacatcctt tttccagtca tacagcgatt tatctgcgat
      481 catcttgcgc atttccaata tcagactggc cttctccacc tttctatggt caaaacacct
      541 tggttttttt gtccgttctt ccctgccgta aataagtatt gactgcctgt tgtcttcaat
      601 tctttccaca atataaaact cgcctgttgc cgtttctttt atcctgccgc ctctggacag
      661 atattttgct gcctgatact gcataaaatc atcaccttta aagtttttta ctcatacaga
      721 cggaatccgg catatccgca taagcgccat agtttggtac gacctgatac cctgctcttt
      781 tatataaagc cattgcctct gtcagcggct cgcctgtctc taaaaccaca gtggaaaacc
      841 ccttgttctt tgcgtttgct tccagccttt ttaacagttc ccctgcaatt ccccgtcctc
      901 tgtattccgg caataaatat aatcttttga tttctgcggt cctttcatca taaaaccgaa
      961 atgctccgca gccgatcggc ctttctttgt catacaccac aattacatcc tgtatttttt
     1021 ccaatgtatt atattttaca tattgctgtc tttgaaaccg accgcctacg atctcatcta
     1081 aattttcatc cagcttccca cacaaataaa caaattcctc gttttttcca tctgtatcaa
     1141 aaaatctcaa actctttact ttctgtcctg caatttgttc agcatcgatt gttggaaaat
     1201 gatagcggcc ctgttttttt tcataatatt ttacggatat cgcctgcttc ggacaataat
     1261 taaagcaggc ataacaataa tagcactcct ctttcgtcca tacaggcttc ttatccaccc
     1321 tgatccggcc tgacagacat actttttcac atattccgca gccattacac gtatcatccg
     1381 aataaaaaac aagttttgga gacgcgctca ttttccacag taactgattc atcatggagt
     1441 cttttctttt tccgttttca acctcttctt taaatacagc caaccggttg ttaacagctt
     1501 cggcgatctg cctgatcgcc tcatttgtat tttgaaccat tttatcaact ttgtcttttg
     1561 taatttccgt ttcccagttc attttcatca aaaatttagg agcgatcccc tttggcgtat
     1621 tacttatcat tttcagactg aaaaactcat cgagccggct gttttttcta tgcagaagca
     1681 tattcagata cccttccggc tttcccggca ttccggcatg tgtcatgata caatacagat
     1741 atgttacctg tcgaaattcc attctcttaa taaaagcttc caccggtttt ggcaaactat
     1801 ttacataaac aggaaacaca attccaataa cgccttctaa gcatctattt tcttctttaa
     1861 gcattggttt tacagctgca tgcacgtatt ttgccagttc ctgcgcagca tacatcgaat
     1921 tgcctgtacc ggaaaagaaa tatatcgtat gcatcaaatc ctccttttca aatcaacact
     1981 cctatgattt cctgatttta gtttaccata tccaatagtt ttattctgcg aattttgtaa
     2041 tataccagct atagccagtg aagtacagct tatgctctcc cttttgccaa aaggaatccg
     2101 ttgttttttc aaaccattga caaacttcgt tcctgctccg tcctttttct tctatctgca
     2161 cggtcacatt tgactgcagc atcataaacc gaataaattg ttctctgctc attttatatt
     2221 ccagcgtata gtcctcctgt ctttccatat gcaaatgaaa tggcgccaca accgcttccg
     2281 accattttgt ttcctttctt gccggcttt
//
LOCUS       DAOSYE010000345         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37111_length_2306_cov_3.426033, whole genome shotgun sequence.
ACCESSION   DAOSYE010000345 DAOSYE010000000
VERSION     DAOSYE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37111_length_2306_cov_3.426033"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1401
                     /locus_tag="PLU43_06640"
     CDS             7..1401
                     /locus_tag="PLU43_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO28125.1"
                     /translation="MKNDNRVVRMNSQNNLLELEEYMYILADVETPNVFRNLFPYEEV
                     PKVAFNDRIVPHNMPKEIWITDTTFRDGQQSRAPYTTDQIVTIFDYLHKLGGPNGMIR
                     ASEFFLYSKKDRDAVYKCMERGYQFPEITSWIRASKEDFQLVKDLGMKETGILVSCSD
                     YHIFYKLKMSRRQAMEHYLNVVKECLETGISPRCHLEDITRADIYGYVIPFCLELMRL
                     MDEYKIPIKIRACDTMGYGVSYSGAVIPRSVQGIIYGLNKHAGVSPHLLEWHGHNDFY
                     KAVSNSTTAWLYGCSGVNTSLFGIGERTGNTPLEAMVFEYAQLKGDLNGMETTIITEL
                     AEYYEKEIGYKIPPRTPFVGENFNVTRAGIHADGLLKNEEIYNIFDTDKFLNRPVLCA
                     VSNTSGLAGIAHWINTYYKLPDERQVAKNSRLVKRIKGEVDAEYESGRVTVMTNQELV
                     SMISQACIDLKITL"
     gene            1436..>2306
                     /locus_tag="PLU43_06645"
     CDS             1436..>2306
                     /locus_tag="PLU43_06645"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOO28126.1"
                     /translation="MDKIKMITPLVEMDGDEMTRILWQMIKEELLIPFIDLKTEYYDL
                     GLKHRDETDDQVTIDSAIATEKYGVAVKCATITPNAARVQEYQLKKMWKSPNGTIRAI
                     LNGTVFRTPIIVKGIEPCVKNWKKPITIARHAYGDIYINTEFLIEKAGKVELLFTGED
                     GTVMRELVHEFPASGGIVRGMHNRTDSIEAFARSCFNYAIDQKQNLWFGTKDTISKKY
                     DHHFKDVFNEIFEAEYKEQFEEAGIEYFYTLIDDIVARIMKSEGGMIWACKNYDGDVM
                     SDMISSAFGSLAMM"
BASE COUNT          711 a          403 c          577 g          615 t
ORIGIN      
        1 atatctatga aaaatgataa tagagttgtc cgtatgaaca gccagaataa tttattggaa
       61 ctggaagaat atatgtatat attggcggat gtggaaactc ccaatgtgtt cagaaacctg
      121 tttccttatg aggaagttcc aaaggttgcc tttaatgatc gtattgttcc gcataatatg
      181 ccaaaggaaa tctggatcac agatacgaca tttcgtgacg gacagcagtc gagggcacca
      241 tatacgactg atcagatcgt aacgatcttt gattatctgc ataagcttgg cggtcctaac
      301 ggcatgatcc gtgcgagcga atttttcctt tacagcaaga aagacaggga tgctgtgtat
      361 aagtgcatgg aacgcggcta tcagtttccg gaaattacaa gctggatccg cgcgagcaag
      421 gaagactttc agctggtaaa agatctgggt atgaaagaaa caggaattct tgtcagctgt
      481 tcggattatc atatatttta caagctgaaa atgagccgcc ggcaggccat ggaacattat
      541 ctgaatgttg ttaaggagtg tctggaaacg ggtatcagcc caagatgtca tttggaggat
      601 attacgcgtg cggatattta cggttatgtg attccgttct gtttggaact gatgcgcctg
      661 atggatgaat ataaaattcc aattaagatc agggcctgtg atacaatggg atacggtgtc
      721 agttattcag gtgcagtcat accgcggtcc gtacagggaa tcatctacgg acttaataag
      781 catgccggcg tcagtccgca tctgctggaa tggcatggcc ataatgattt ttataaagca
      841 gtctcgaatt ccactacagc atggctgtat ggttgttccg gtgtaaatac ttctctgttc
      901 ggaattggtg agcggacggg aaatacacct cttgaggcaa tggtatttga gtatgcacag
      961 ctgaagggtg atctgaatgg catggaaacg acgatcatta cggagcttgc cgaatattat
     1021 gagaaggaga tcggttataa gatccctccc cgtacaccgt ttgtcggcga aaattttaat
     1081 gtgacccgtg ccggtattca cgcggacggt cttttaaaaa atgaagaaat ttataatatt
     1141 tttgataccg ataagttttt gaacaggcct gtactctgtg cagtatcaaa cacttccgga
     1201 cttgcgggaa tcgcccactg gatcaataca tactataaac tgccggatga acgtcaggtt
     1261 gctaagaatt caaggcttgt aaagcgtatt aaaggtgaag tggatgccga atatgagagc
     1321 ggcagagtaa ctgttatgac aaatcaggaa ttggtcagca tgatttcaca ggcatgtatt
     1381 gatttgaaaa ttacattata aagatgaact taataaagaa aggattacgt ggtaaatgga
     1441 taaaattaaa atgataactc cccttgtaga aatggatgga gacgagatga cgaggatttt
     1501 gtggcagatg ataaaagagg agctgttgat tccatttatc gatttaaaga cagaatatta
     1561 cgatttggga ttaaagcaca gagatgaaac cgatgaccag gtaacaatag attccgcaat
     1621 agcgaccgag aagtacggtg tggctgttaa gtgtgcgacg atcacgccga acgcggcacg
     1681 tgtacaagag tatcagctga aaaaaatgtg gaagagtcct aatggtacga tccgtgccat
     1741 attgaacgga actgttttcc ggacaccgat tattgtaaag ggcattgaac catgcgttaa
     1801 gaactggaaa aaaccaatta cgatcgcaag acatgcttat ggtgatattt acatcaatac
     1861 ggaatttctg atcgaaaaag cagggaaagt cgaattgctg tttacgggtg aagacggaac
     1921 agtcatgcgc gaactggtcc atgagtttcc tgcatccggc ggtattgtac gcgggatgca
     1981 taaccgtacg gattccatag aagcatttgc acgaagctgt tttaactatg cgatcgacca
     2041 aaaacaaaac ctgtggtttg gaacaaagga tacaatatcg aaaaagtatg accaccattt
     2101 caaggatgtt tttaacgaga tctttgaagc tgaatataag gaacagtttg aagaagccgg
     2161 tattgagtac ttttatacgc tgatcgatga tattgtagcc agaatcatga aatcagaggg
     2221 cgggatgatc tgggcgtgta aaaattatga tggagatgtc atgagcgata tgatctcatc
     2281 cgcgtttggc tcccttgcaa tgatga
//
LOCUS       DAOSYE010000346         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37165_length_2305_cov_2.288444, whole genome shotgun sequence.
ACCESSION   DAOSYE010000346 DAOSYE010000000
VERSION     DAOSYE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37165_length_2305_cov_2.288444"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLU43_06650"
     CDS             <1..454
                     /locus_tag="PLU43_06650"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOO28127.1"
                     /translation="FANTISVVEVTGQQILDALEMGAREWPEENGGFLQVSGMSYEID
                     TQIPSSVKTDDTGAFIGVEGEYRVKNVKISGRDLETEKKYVLSATDYILRDCGDGYTM
                     FTEAKRINESFILDSEAVIEYVSNGLNGHIGKEYEDPYGEGRIIETGE"
     gene            490..735
                     /locus_tag="PLU43_06655"
     CDS             490..735
                     /locus_tag="PLU43_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HOO28128.1"
                     /translation="MKNSAKYFENRDCKYYPCHQGLEEMNCLFCYCPLYHLEECPGNP
                     DFIVKDGKKIKCCINCTFPHRAENYELIMQYLKQELQ"
     gene            complement(767..1489)
                     /locus_tag="PLU43_06660"
     CDS             complement(767..1489)
                     /locus_tag="PLU43_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO28129.1"
                     /translation="MKNLLKAAGTKSNPFSLKEFLSSYRFGLIIPLYAAIYLPWFCYL
                     EEKVTKRYHIIHMAIDDYIPFCEVFIIPYFLWFVYMTVSIVLCMVTNKESFFRSFFFL
                     SCGMTIFLIVSTVFPNGQHLRPTVFPRDNFLTDMVKKLYSIDTSTNLFPSIHVYNSIG
                     SHIAIVKNETLRKKKWLKNSSFILCILIILSTIFLKQHSMFDVLTAIGLAVIMYPIAY
                     KIDYAAWAARKRSAGEKEHAGA"
     gene            complement(1536..1805)
                     /locus_tag="PLU43_06665"
     CDS             complement(1536..1805)
                     /locus_tag="PLU43_06665"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28130.1"
                     /translation="MFCPDADYKDGFLDICVAGNLSKPNVLRILPTAYKGNHVHYKGI
                     DNYRAKKVTVVADTPSPVHADGESCGIRTEITVTQDIKQLRVIIG"
     gene            complement(1766..>2305)
                     /locus_tag="PLU43_06670"
     CDS             complement(1766..>2305)
                     /locus_tag="PLU43_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOO28131.1"
                     /translation="EITSGTEPVTIIVLGGDGTINEVLTGIRDYDLVTLGYIPAGSSN
                     DLARDLMLARNPALALQAVLHPKEYTYMDVGVIRYQDDGKNFGVSAGIGYDAAICHEA
                     LHSGMKDFLNKLHLGKLTYVGIALKQLAKTKNSGCTVILDDSRKLRLTGFILLHAWYI
                     NMKAADLCSARMRIIKTDF"
BASE COUNT          706 a          451 c          529 g          619 t
ORIGIN      
        1 gtttgccaat acgatcagtg tagttgaagt gaccggacag cagattctgg atgcgcttga
       61 aatgggcgca agggaatggc ccgaggaaaa cgggggcttt ttgcaggtct ccggtatgtc
      121 gtatgaaata gatacacaaa taccgtcttc ggtcaaaaca gatgataccg gggcatttat
      181 cggtgtagaa ggagaatacc gtgtaaaaaa tgtgaaaata agcggaaggg atcttgaaac
      241 ggaaaagaaa tatgtgctgt ctgcaacaga ttatatattg cgtgactgcg gggatggcta
      301 tacgatgttt acggaagcaa aaagaatcaa tgagtcgttt attctggaca gtgaagccgt
      361 tattgaatat gtatcgaatg gtttgaacgg gcacatcgga aaagaatacg aagatccgta
      421 cggagaagga agaattatag agaccggtga gtgaatctgg ccggaatatc agattgggat
      481 gataaaatta tgaaaaacag tgcgaagtat tttgaaaaca gggattgcaa gtactatccg
      541 tgtcatcagg gtcttgaaga aatgaattgt ctgttctgct actgtccgct gtatcatttg
      601 gaagaatgtc cggggaatcc tgattttatt gtaaaagacg gtaaaaagat caaatgctgc
      661 ataaactgca cgttcccgca cagagctgaa aattatgagc tcattatgca atatttaaag
      721 caggaactgc agtgagtgaa acattttccg gcatgctccg aaaagattac gcaccggcat
      781 gctctttttc tccggccgac cgcttgcgtg cagcccaggc cgcataatcg atcttgtagg
      841 cgatcggata catgatgact gcaagtccga tcgcagtcag aacgtcgaac atggagtgct
      901 gttttaaaaa gatcgtcgat aaaatgatca aaatacataa aataaaggaa ctgttcttca
      961 gccatttctt ttttcggagc gtctcatttt ttacgattgc aatatgggaa ccgatcgaat
     1021 tatatacatg aatacttgga aacagatttg ttgaagtatc aatggaatat aattttttca
     1081 ccatatcagt cagaaaattg tctctcggga agacagttgg tcttaggtgc tgcccattcg
     1141 ggaagacggt tgaaacgatc agaaaaattg tcatgccgca ggagagaaag aaaaagcttc
     1201 tgaaaaaaga ttccttattt gtgaccatgc acagaacaat ggaaacagtc atgtagacaa
     1261 accacagaaa atatggaata ataaatacct cacaaaatgg aatgtaatca tcaatggcca
     1321 tatgaatgat gtggtatctt tttgtaacct tttcctccag ataacaaaac catggaaggt
     1381 agatcgcagc gtaaagcgga atgatcagtc cgaatcgata ggacgaaaga aattctttca
     1441 gggaaaaagg gtttgacttg gtcccggctg ctttcaataa atttttcata atatattcac
     1501 ctctgtattc tcctcaaaat aacagtgtta tttaattatc cgataattac acgcagctgt
     1561 ttgatatcct gcgtgaccgt gatttctgtt cggatgccgc atgattctcc gtcggcatgt
     1621 acgggagacg gtgtgtccgc aacaacagtc actttttttg cgcgatagtt gtcaattcct
     1681 ttataatgga catgatttcc cttgtaagct gtcggaagga tacgcaggac atttggcttg
     1741 gaaagatttc cggcaacgca gatatctaaa aatccgtctt tataatccgc atccgggcag
     1801 aacataaatc cgccgccttc atatttatgt accatgcatg taacaaaata aaaccggtca
     1861 aacgtaattt acgggaatca tccaaaatta cggtgcagcc gctgtttttc gtttttgcga
     1921 gctgctttaa ggcgataccg acataggtca gtttcccaag atgcagttta tttaaaaaat
     1981 ctttcatacc ggaatgcaaa gcctcgtgac agatcgcagc atcgtatccg atacctgcgc
     2041 tgaccccgaa atttttaccg tcatcctgat aacggataac gccgacatcc atatacgtat
     2101 attccttcgg atggagaact gcctgtaggg ccagggcagg attgcgcgcc agcatcagat
     2161 ctcttgcgag atcattgctg gaacctgccg gaatataacc cagggtaaca agatcataat
     2221 ccctgatgcc ggtcagtact tcattgatcg taccgtctcc tccgagaaca ataatagtaa
     2281 ccggttctgt tccggatgtg atttc
//
LOCUS       DAOSYE010000347         2303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37201_length_2303_cov_2.922153, whole genome shotgun sequence.
ACCESSION   DAOSYE010000347 DAOSYE010000000
VERSION     DAOSYE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37201_length_2303_cov_2.922153"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLU43_06675"
     CDS             complement(<1..270)
                     /locus_tag="PLU43_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO28132.1"
                     /translation="MALFKSTKKEEKTEGQEASVKVLGSGCAKCNQLEAAAKEALKAL
                     GMDTTVGHVTDFAKIAAYGVMSTPALVVDEKVVSYGKVLKADEVME"
     gene            complement(329..1474)
                     /locus_tag="PLU43_06680"
     CDS             complement(329..1474)
                     /locus_tag="PLU43_06680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO28133.1"
                     /translation="MSTFLYVELNLVGILLLLLMLTSIHGEARRDISPDQFLFHGVML
                     LNLFIFVFDTVMWLIDGRMFAGARVLNYIASTCYYLLNALICFWWLIYTDYKIYESRH
                     GLIRRVRYYMIPFLFCAVGSLLTPWTGWFFVIGSDNRYSRGPFFWFMVACAFLYLLGS
                     FGITVYDILKNGLEERRGMNIYLVLFPIIVLIAVVIQSLFFGISIIWTCTMLIAVSIY
                     IYIQNGEINNDYLTGLYNRRRMDQYLIRKIKVRKQDRLLFLVVLDVNNFKRINDTYGH
                     LAGDCALKKAAEILRAVCAGTDDFIARMGGDEFVIIGERNTEEEIRGLIRKIREQENK
                     KEQTEQADYQLSFSIGYSVFREEHTPDSFLAEADHMMYEEKQERKTY"
     gene            1625..2299
                     /locus_tag="PLU43_06685"
     CDS             1625..2299
                     /locus_tag="PLU43_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOO28134.1"
                     /translation="MMQTYVCGICGFVYDEAAGYPEGGIAPGTRWEDLPDDWVCPLCG
                     ASKDVFELQNKTEPASSSKKADSTETLSDENLSAKELSALFSNLSKGCEKQYRQEEAT
                     LFRRLEDYYGQKTAPEKESQLSALTKLAKDDLSSGFEDGQQAASSSHDRGAMRALTWS
                     EKVTKNTAFLLNRYEAHTGPALEQTNIYVCEICGFIYVGEKAPEICPVCKVPKQKLKL
                     IPKGVA"
BASE COUNT          673 a          580 c          424 g          626 t
ORIGIN      
        1 ttccataacc tcatccgcct ttaaaacttt accataagaa accacttttt catctaccac
       61 aagtgccgga gtcgacatca caccatacgc tgcgatcttg gcaaaatccg tcacatggcc
      121 gaccgtcgta tccattccaa gtgctttcag ggcttccttt gctgctgcct ccagctgatt
      181 acactttgca catccggaac caagcacctt taccgatgct tcctgccctt ctgttttttc
      241 ctcttttttc gtagatttaa acaatgccat ttcaattcct cctatacgat atctgttgct
      301 attactatat ctctcaaatc gataattgtc aatatgtctt tcgttcctgt ttttcttcat
      361 acatcatatg gtccgcttcc gccaaaaacg aatccggcgt atgttcttca cgaaaaacgg
      421 aatacccaat actgaacgat aactgataat ccgcctgttc tgtttgctct tttttatttt
      481 cctgttccct gatttttctg atcagccccc tgatttcctc ctccgtatta cgttccccga
      541 tgatcacaaa ctcatcgccg cccattctgg caataaagtc atccgttccc gcacatacgg
      601 cccttaatat ctccgcggct ttttttaatg cacaatcacc cgcaaggtga ccgtatgtat
      661 cattgatccg tttgaaatta ttcacatcaa gaactacaag aaacagcagc cggtcctgct
      721 ttcgtacttt gatcttcctg atcagatatt gatccatacg gcgtctgtta taaagtcctg
      781 tcaaataatc attattgatc tcgccgtttt gaatataaat ataaatgctc accgctatga
      841 gcattgtaca tgtccatatg atcgatattc caaaaaacag ggattgtatg acgactgcga
      901 tcaaaacgat gattggaaac agaacaagat aaatattcat gcccctgcgc tcttccagac
      961 catttttcaa aatatcatac acagtgattc cgaaagaacc aagcagataa agaaacgcac
     1021 atgctaccat aaaccagaaa aaaggcccgc ggctgtaccg attgtcggag cctatcacaa
     1081 aaaaccagcc ggtccacggc gtaagcagac ttccgaccgc gcaaaaaaga aagggaatca
     1141 tataatatct aaccctgcgg atcagaccat gtctgctctc atatatttta taatccgtat
     1201 aaataagcca ccaaaagcag atcagtgcat tcagcagata atagcaggtc gatgcaatat
     1261 aattgagtac tctcgctccc gcaaacattc ttccgtctat gagccacatt accgtatcaa
     1321 atacaaaaat aaaaaggttc agaagcatca cgccatgaaa aagaaattga tccggagaaa
     1381 tgtctcttct tgcctctcca tgtatgcttg tcagcatcaa aagcaaaagc agtataccga
     1441 ccagattcag ttcgacatat aaaaacgttg acataatatc ctctttcctt ctgcactgac
     1501 tgtccatcat ggttcgtatc agattatatc ataggaaagc agttttttct atatgatttc
     1561 cgcctgaaat agcgtaaact gttgttatat tcacattttt attcatttca aaaaggagga
     1621 ctgaatgatg caaacttatg tatgtggtat ttgtggtttt gtctatgacg aagctgccgg
     1681 ttatccggaa ggaggcatag cgcccggaac caggtgggaa gatcttcccg acgactgggt
     1741 ctgcccgtta tgcggcgcct caaaggatgt ctttgaattg cagaacaaaa cagaaccggc
     1801 ctcttcttcc aaaaaggcag acagtacgga aacattatcc gacgaaaatt tatctgcaaa
     1861 agaactatcc gccctgtttt caaacctctc aaagggatgc gaaaaacaat accggcaaga
     1921 ggaagccacc ctgtttcgcc ggctggagga ctattacggc cagaagaccg ctcctgaaaa
     1981 ggagtcccaa ctgtctgcgc tcacaaagct tgcaaaagac gatctttctt ccggatttga
     2041 agacggacag caggcagcgt cctcttccca tgaccgcggc gccatgcgcg cactgacctg
     2101 gagtgaaaag gtaacaaaaa atacagcctt cctgctgaac agatatgaag cccataccgg
     2161 tcctgctctt gaacagacca atatttacgt atgtgagatc tgcggtttca tctatgtcgg
     2221 agaaaaagct cctgaaatct gtccggtatg caaggttccg aaacagaaac tcaaactgat
     2281 tccaaagggg gtggcttaaa tgt
//
LOCUS       DAOSYE010000348         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37378_length_2297_cov_2.272079, whole genome shotgun sequence.
ACCESSION   DAOSYE010000348 DAOSYE010000000
VERSION     DAOSYE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37378_length_2297_cov_2.272079"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLU43_06690"
     CDS             <1..444
                     /locus_tag="PLU43_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28135.1"
                     /translation="KILHLTGTYAGVIFVESAVQLPWTVFTLTGFIKNVPKELDEAAF
                     IDGAAPLHMFFKIVLPLMKPILATALVSAAMYAWNEFMVPLYFFNTSSNWTLPLTVYN
                     FFGQYASNWNYVFADLMITALPITILYLCCQKYIVSGATAGAVKG"
     gene            459..>2297
                     /locus_tag="PLU43_06695"
     CDS             459..>2297
                     /locus_tag="PLU43_06695"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28136.1"
                     /translation="MGDLRFRQVHLDFHTSEYLTDIGKKFDKLQFQQTLQMGHVNSIT
                     LFSKCHHGWSYHPTTVNQMHPHLTIDLLQEQMLACREIDVRTPVYLSAGLDEKEAVRH
                     PEWVARQADETTTWVKDFTSEAGFHLLCFNTGYLDLLLRQIEEVMRVYHPPGIFLDIA
                     AVHPCYCSKCRREILERGKDPRDMEAVMEQAEKVYASYTDKVEQMIRKYSSSCTIFHN
                     AGHITRGRRDLAACNTHLELESLPTGGWGYDHFPMSAAYVSNLSMEYLGMTGKFHNTW
                     GEFGGFKHPNALRYETSLSLAFGAKCSIGDQLHPSGCLDHDTYELIGRAYREVEKKEP
                     WCRDAKNCYDIGVLGEEAVHTKISDRNQKRYADIGANRILLEGKFLYRFLDLQSDFSR
                     CKVLILPDRIQPDEKLQRRLQEYLRDGGKILASGRSGTEPSSDRFVLDFGAEFRGPIS
                     FRPDYLIPAFPLSTGQSPHAMYEQGYEIVMKNGKQMGSREYPYFNRDVFHFSSHQHTP
                     NDPDQKWPSISMTERTAYIGWDIFTDYAKMGSLHLKETVIYLLDCLLGDRKMIQASLP
                     DRGVCTLTWQKKEQRFITHLLFAHTSVRGQFLWDGELHPIEVIESIV"
BASE COUNT          649 a          412 c          585 g          651 t
ORIGIN      
        1 aaaatactgc atctgacagg aacctatgca ggagtgatct ttgttgagtc ggcagtacag
       61 ctgccatgga ctgtctttac cctgaccgga tttattaaaa atgtaccaaa ggaattggat
      121 gaagctgctt ttatagatgg tgctgcgcca ctgcatatgt tttttaagat tgtacttcca
      181 ctgatgaagc cgatattggc gactgcatta gtatctgcgg caatgtatgc atggaatgag
      241 tttatggttc ctttgtattt ctttaataca tccagcaatt ggacgcttcc tctgacagtg
      301 tataatttct ttggacagta tgccagcaat tggaattatg tatttgctga tttgatgatt
      361 acggcattac caatcactat tttatatttg tgctgtcaga aatatattgt ttccggagca
      421 actgccggtg cggtaaaggg ataataggag gagaaaatat gggagatctt cgattccggc
      481 aagtccattt ggacttccat acatcagaat atcttacaga cattgggaaa aagttcgata
      541 agctgcaatt tcagcagacc ttgcagatgg gacatgtaaa ttctattact ttattttcaa
      601 aatgccatca tggatggtca tatcatccga caacagtaaa ccaaatgcat ccgcatctga
      661 cgatcgatct tttacaggag cagatgctgg cgtgccggga aattgacgta agaacgcctg
      721 tctatctgtc ggccggtctg gatgagaaag aagctgtcag gcatccggaa tgggttgcga
      781 ggcaggcaga tgaaacaact acctgggtga aggattttac ttcggaagct gggtttcatt
      841 tgttatgctt taatacagga tatcttgatc tgctgcttag acagattgag gaggtcatgc
      901 gtgtgtatca tccgccgggc atttttttag atattgctgc cgtgcatccc tgttactgtt
      961 caaaatgccg tagagagatt ctggaaagag gcaaggatcc aagagatatg gaagcagtga
     1021 tggaacaggc agaaaaggta tatgcgtcat atacggataa agtggagcaa atgatcagaa
     1081 aatatagctc cagttgtacg atttttcata acgccggtca tattacaaga ggacgcagag
     1141 atctggcggc ctgtaataca catcttgaat tagaaagtct gccgaccggt ggctggggat
     1201 atgatcattt tccgatgtcg gcagcatatg tatccaatct ttcgatggag tatcttggaa
     1261 tgacaggaaa atttcataat acatggggag aattcggtgg ctttaagcat ccgaatgcat
     1321 tacgctatga aaccagtctg tcgttagctt ttggagcaaa atgttccatt ggggatcagc
     1381 ttcatccttc aggatgtctg gatcatgaca catatgaact gatcggcaga gcttatcggg
     1441 aagtggagaa gaaggaacca tggtgtcggg atgcaaagaa ttgttatgac attggtgtgt
     1501 tgggagaaga ggctgttcat acgaaaattt cggatcgaaa tcagaaacgt tatgcggata
     1561 taggagcaaa tcggattctg ctggagggta agttcctata tagattcctt gatctgcagt
     1621 cggattttag tcgatgtaag gtgttgattc ttccagatag aatacaaccg gatgagaagc
     1681 tgcaaagacg cctgcaggaa tatttacggg atggtggaaa gattcttgct tcaggaagaa
     1741 gcggaacaga gccttcatcc gatcgatttg tattggattt tggagcggaa ttcagaggtc
     1801 ccatttcatt caggccggat tatctgattc ctgcgtttcc attgagcacc ggacagtctc
     1861 cacatgcgat gtatgaacag ggatatgaga tcgtgatgaa aaatggaaaa caaatgggaa
     1921 gcagggagta tccgtatttc aatagagatg tctttcattt tagctcacat cagcatacgc
     1981 cgaatgatcc ggatcagaaa tggccatcta tatctatgac agagcgtacg gcatatatcg
     2041 ggtgggatat tttcacagat tatgcaaaga tgggttctct tcatctgaag gaaacggtga
     2101 tctatctgtt agactgtctg cttggagata gaaagatgat acaggcatct ctgcctgatc
     2161 gtggagtctg tacgctgacg tggcagaaga aggaacagcg attcattaca catttgcttt
     2221 ttgcgcatac gtccgtcaga ggacagtttt tatgggatgg ggaactccac ccaattgagg
     2281 taatagagag catcgtt
//
LOCUS       DAOSYE010000349         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37461_length_2293_cov_4.198391, whole genome shotgun sequence.
ACCESSION   DAOSYE010000349 DAOSYE010000000
VERSION     DAOSYE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37461_length_2293_cov_4.198391"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLU43_06700"
     CDS             complement(<1..1173)
                     /locus_tag="PLU43_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO28137.1"
                     /translation="MGTKHTLEELNNCSREELIAYVLMMQGQLDTLNENIEKLIEQVR
                     LANQYRFGRHTEKLDAIDGQISFFDEAESLCDEKAAEPEMEDVLPSKPKKKKAKGRRE
                     EDLKDFPQDIKAPYTVSVEELDSFYGVGNWKKMPDETYKRLRHEPESWTVEIHTVEVY
                     VGTDGDHQDEFLRGKRPKDLLRNSIVTPSLLSSIVNVKYVNSSTLNRVEQEFSRNGVN
                     ISRQTMSNWIIKSTDKYLCPLWDRLQEELLKFHVNQSDETPTQVIHDGKSAGSKSYMW
                     VHRSGEFYTETPIVLYEYQKGRDHSLPLEFYRDYKGILVTDSLEQYHLVDKKLPDVTN
                     ANCWAHARRSYADAIKAADKKDPDAVKKSVAYQALSRLSLIYKLEGTLKDFSPKERL"
     gene            complement(1448..1804)
                     /gene="tnpB"
                     /locus_tag="PLU43_06705"
     CDS             complement(1448..1804)
                     /gene="tnpB"
                     /locus_tag="PLU43_06705"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOO28138.1"
                     /translation="MLEDAAGIRRVVLACGTVDLRKGIDGLSMIIGDKYNQNPFEKGT
                     LFLFCGRRSDRIKGLLWMGDGFLLLYKRFEAGHMSWPRTTQEAAELTEEQYHYLMLGL
                     NPLDPKIKEVHPRKLY"
     gene            complement(1797..2183)
                     /locus_tag="PLU43_06710"
     CDS             complement(1797..2183)
                     /locus_tag="PLU43_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOO28139.1"
                     /translation="MDQCTHEVRAQYWKEIIEACQQRPVGQSAKQWLKENGILEQSYY
                     KWQRKFRKETFELMQATDLNVTPAQKNNISFAEISVKQDKTENCLETFVSPTAVIKTP
                     SMTIAISNEISDSLLSRIMREVSADA"
BASE COUNT          557 a          521 c          454 g          761 t
ORIGIN      
        1 aagtctttcc ttgggcgaaa aatcctttag tgtgccttcc agcttataga tcaacgagag
       61 cctgcttaat gcctgatatg ccacggattt tttcacggca tctggatctt ttttatctgc
      121 tgcttttatc gcatctgcat agcttcgtct ggcatgtgcc cagcagttgg catttgttac
      181 atccggcagt tttttatcta ccagatgata ctgctccaga ctgtccgtta ccagaatgcc
      241 tttataatcc cgatagaatt ccaatggcag actgtgatca cgtcccttct ggtattcgta
      301 caggacgata ggcgtttccg tgtaaaattc ccccgaccgg tgtacccaca tgtaactttt
      361 acttccggca ctttttccat catggatcac ctgggttggc gtttcgtccg actggtttac
      421 atggaatttc agaagttctt cctgcagtct gtcccataac gggcacagat acttatccgt
      481 acttttgatg atccagtttg acatggtctg tctggagata tttactccgt ttctggaaaa
      541 ctcctgctca acacggttga gggtagagga atttacatat ttcacattta cgatagagga
      601 aagcagtgaa ggagtcacta tactgtttct gagcagatct tttggtcttt ttccacgcag
      661 gaattcatcc tggtggtcac cgtctgttcc tacatatact tcaacagtat gaatctccac
      721 tgtccaggat tccggctcat ggcgtaatct tttataggtt tcatccggca tctttttcca
      781 gttccccaca ccataaaagg aatccagttc ctccacggaa acggtataag gagctttgat
      841 atcctgtggg aaatccttaa gatcttcttc tcgccggcct tttgcctttt tcttctttgg
      901 tttagatggc agtacatctt ccatttctgg ttcagcggct ttttcatcac aaagtgattc
      961 agcttcatca aaaaaggaaa tctgtccatc tatggcatcc agcttttctg tatggcgtcc
     1021 aaaacggtac tgattagcaa ggcgtacctg ctcgatcagt ttttcaatgt tctcattcag
     1081 tgtgtcaagc tgtccctgca tcatgagaac atacgcgata agttcttcac ggctgcagtt
     1141 attcagttct tccagtgtat gctttgtacc cattgtctgt ccgctccttt attaatcctt
     1201 attatagcgg aaatgatgga aaatggcgaa ttttccagcc tgcccggttc gtcattttga
     1261 ctatggataa aaccttgtaa aaagctttct cgggaatgga aatatagagt tttcaaggtt
     1321 caggcagtta ttgattccat tctgaaatca tcatgtaaag cggcataaag acctgcattt
     1381 atcatgcttt tacattgttt atatattgaa aaaacaggca gaaaaatttc tctgttttta
     1441 acaatcatta gtatagtttt cttggatgga cttcctttat cttgggatcc agcgggttta
     1501 agcccagcat cagataatgg tactgttctt ctgtaagttc agctgcctcc tgtgtagtgc
     1561 gcggccatga catatgtcca gcctcaaagc gtttgtataa gagcagaaag ccgtcgccca
     1621 tccataatag ccccttgatt ctgtcactcc ttcttccaca gaagagaaac agtgtcccct
     1681 tttcaaaggg attttgattg tatttgtcac caatgatcat cgacaggccg tcaatgcctt
     1741 tacgtagatc aacagtccca caggcaagga cgacacgtcg gattccagct gcatcttcaa
     1801 gcatctgcgg atacctccct catgattctg gatagtaagg aatccgatat ctcattcgaa
     1861 atggcaatgg tcattgatgg ggtttttatt acagcggtgg gggaaacgaa tgtttctaaa
     1921 cagttttctg tcttgtcttg ttttactgaa atttcggcaa atgaaatatt gtttttctga
     1981 gcaggtgtaa cgtttaaatc tgttgcctgc attaattcaa aagtttcctt tcggaatttc
     2041 cgctgccatt tgtagtagct ttgttcaaga atcccatttt ctttcagcca ctgctttgcg
     2101 ctttgcccca ccggacgctg ctggcaggct tcaataattt ctttccaata ctgtgctctt
     2161 acctcatgag tgcattgatc cataaataaa gtacctccta ctcgcaaaaa tcaattgttt
     2221 tttacgagta tgcactattt tgaatattct tgtacactga aatcacaaat ccggattgtt
     2281 ggaaatcgat att
//
LOCUS       DAOSYE010000350         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37742_length_2282_cov_4.097890, whole genome shotgun sequence.
ACCESSION   DAOSYE010000350 DAOSYE010000000
VERSION     DAOSYE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37742_length_2282_cov_4.097890"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLU43_06715"
     CDS             <1..632
                     /locus_tag="PLU43_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO28140.1"
                     /translation="AKTAGIPYVSFLTSVTGCDDYDGYIYVGSPNYDAGQAQGEYLCD
                     ALPENAQILYFTGEPNDQQYIDRKQGLEEALAARDDITILDEYNVKNSKDQGMSTTED
                     CLLSYDSFDAIVCQNDDAALGVVEALKSADRLDGSILVLGVDGSDEALQSIKNGEMAM
                     TALQDAKAQAEAGADIFEQIKNGTDPADIDDVYIPFKIVTSDNVDDYIE"
     gene            736..>2282
                     /locus_tag="PLU43_06720"
     CDS             736..>2282
                     /locus_tag="PLU43_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOO28141.1"
                     /translation="MYKYKNAELPIEERLDDLMERMSFEQKIDQITCLVTITEEIPDF
                     KEYIPAGIGNVGAFTVSDDIEEIAEYVHKLQKFLMEETPLGIPALIHCEAAAGAQFTE
                     ANVFPSAIAQASTFDLHVISEMADIIRRQMLSVGFRQALSPVVDITRDPRWGRITETY
                     GEDPTLTAAMASVFVKGLQTENLREGLIATAKHYVGHGITEGGLNMGRNLVLERDLLE
                     IHCKPFQCAITEAGLGSVMNSYCSINGEPVVGSKKLLTDILRGEMGFEGFVVSDYLSV
                     DRMVDPFCVADSYEDAGIKAINAGLDVEYPRPKGFCYQMKKAVENGTLRMDTIDRAVR
                     RVLKAKFELGLFENPYPELTGLKKVLHMETTDKLNEKIAMESLTLLKNENGLLPLSKE
                     KKKIAVIGPHGDSLRSYFGTFSYPAVLDMITAREEDGQEFEEPGLIIYDIKQKYRGQI
                     REVSPKIERRLQKEFPRSRTLKKALQEYVPACEIAYAKGINCSGSDIGGMQEAIDLAA
                     QADVVILT"
BASE COUNT          692 a          421 c          622 g          547 t
ORIGIN      
        1 cagcaaaaac tgcggggatt ccgtatgttt catttttgac atctgttaca ggctgcgatg
       61 attatgacgg ttatatttac gtaggatctc caaattatga cgcaggtcag gcacagggtg
      121 aatatttgtg tgatgcgctg ccggaaaatg cacagatctt atattttacc ggagagccga
      181 acgaccagca gtatattgac agaaaacagg gattggaaga agcgcttgca gcaagggacg
      241 acattacgat tctcgatgag tataacgtaa aaaattccaa agaccagggg atgagcacaa
      301 cagaggactg cctgctgtca tatgattcgt ttgacgcgat cgtctgccag aacgatgatg
      361 cggcacttgg tgttgttgaa gctttaaagt cggcggatag actggacgga agtattttag
      421 tgctgggcgt ggatggaagt gatgaagcac tgcagtcgat caaaaacgga gaaatggcaa
      481 tgactgcatt gcaggatgca aaggcacagg ccgaagcggg agccgatatt tttgagcaga
      541 tcaaaaacgg aacggacccg gctgatatcg atgatgtgta tattccgttt aaaattgtaa
      601 caagcgacaa cgttgacgat tatatagagt aacataataa gcgttagatg aaatacacag
      661 gtgttcatat aaaagcgcct gaccgtttca gggtcaggcg catgctataa aatcataaga
      721 aagagaccag tgacgatgta caaatataaa aatgcggaat taccaataga agagagactt
      781 gatgatttaa tggagagaat gtcgtttgag cagaaaatag accagataac ctgtctggtg
      841 acaattaccg aagaaattcc ggattttaag gaatacatac cggcgggaat tggaaatgtc
      901 ggtgcattta cggtgtctga cgatattgaa gaaattgcgg aatacgtgca taaactgcag
      961 aagtttctca tggaggaaac cccgcttggg attcctgcgc tgatccactg tgaagcagcg
     1021 gccggcgcgc agtttacgga agcaaatgtt tttccgagtg caattgcaca ggcctctacc
     1081 tttgatctgc atgtgatttc tgaaatggcg gatattatcc gcaggcagat gttatctgtc
     1141 ggtttccggc aggcattgtc accggtcgtt gatattacaa gagatccaag atggggaagg
     1201 atcacagaga cttatgggga agacccgacg ctgacggctg caatggccag tgtttttgta
     1261 aaaggactgc agacagagaa tctgcgggaa ggactgattg caactgcaaa gcattatgtc
     1321 ggccatggga ttacggaagg cggtctgaat atgggcagaa atctcgtttt ggaacgggac
     1381 ctgctggaga tccactgtaa gccgtttcaa tgtgcaatta cagaagcagg cctgggcagt
     1441 gtaatgaatt cctattgcag tatcaatgga gagccggtcg tcggctcaaa aaagctgctg
     1501 accgatattc tgcgtggtga aatgggcttt gaaggatttg tcgtttccga ttatctgtcg
     1561 gtagaccgga tggtcgatcc gttctgtgtt gcggattcct atgaagacgc gggaatcaaa
     1621 gcaatcaacg ccggactgga tgtagagtat ccaagaccaa aggggttttg ctatcagatg
     1681 aaaaaggcag tggaaaacgg aacgcttcgc atggatacaa tcgatcgagc ggtcagacgt
     1741 gttttaaagg caaaatttga gctgggtctg tttgaaaatc cttatccgga gctgaccgga
     1801 ttaaaaaagg ttctgcatat ggaaactacg gataagctga atgaaaaaat tgccatggaa
     1861 agtctgactc tgttaaaaaa tgaaaatggg ctgcttccct tatcaaagga gaagaaaaaa
     1921 atagcggtca tcggtcctca tggggacagc ttaagaagct attttgggac attttcttat
     1981 cccgcagttt tagatatgat aacagcacgc gaagaagacg gacaggagtt tgaagagccg
     2041 ggtctcatta tttatgatat caaacagaaa tacagaggac agatcaggga agtatcgcct
     2101 aaaatagaac gcagactgca gaaagaattt ccccgctcgc ggacactgaa aaaagcactg
     2161 caggaatatg ttccggcatg tgagattgcc tatgcgaaag gaatcaactg ttccggcagc
     2221 gatatcggcg gtatgcagga agccatagat ctggcagcac aggcggatgt ggtaattttg
     2281 ac
//
LOCUS       DAOSYE010000351         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37783_length_2281_cov_2.685984, whole genome shotgun sequence.
ACCESSION   DAOSYE010000351 DAOSYE010000000
VERSION     DAOSYE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37783_length_2281_cov_2.685984"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2281)
                     /locus_tag="PLU43_06725"
     CDS             complement(<1..>2281)
                     /locus_tag="PLU43_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOO28142.1"
                     /translation="HKKSGARVLLLSNDDENKVFSIGFRTPPYDSTGLPHILEHSVLC
                     GSKLFPAKDPFVELAKGSLNTFLNAMTYPDKTIYPVASCNDKDFQNLMHVYLDAVFYP
                     NIYKREQIFRQEGWHYELEEKNDEITINGVVYNEMKGAFSSPDDMLDREVFNTLFPDT
                     AYSYESGGDPEVIPTLTYEKFLDFHRKYYHPSNSYIYLYGNADMAEKLTFIDEAYLSK
                     YEEAAISSKIELQKPFDEPVRKTKKYSITNEETEEDNTYLSYNAVIGTSLDRELYVAF
                     QIIEYALLLAPGAVLKQTLLDRGIGKDIQSTYENGVLQPYFSVIAKYANTEQLDTFLD
                     VIKEVLSNVVQNGIDQKALLAGINYFEFKYREADFGSYPKGLMYGLQAFDSWLYDDAL
                     PFIHIEANETFAKMREYVGSGYFEKLVETYLLHNTHASVVVVEPERGLTAKLDKKLAD
                     QMQAYKESLSEEETERLIADTKALIAYQEEPSTKEELLSIPLLKISDIKEEAQPYIYA
                     EHRLDRTILLTHDIFTNGIGYLTLGFDTGKLPAGMLPYLGVLKNVLGFVSTKNYTYAD
                     LFNEININTGGITMGAVTYVESAHPDNYRTMFEVRAKVLFDKLPFAFEMIKEILFTSD
                     FSDEKRLKEILAMLKSRLQTTMVSAGHSTAALRAMSYCSETASVSEQIGGVTFYRLIE
                     LLEAHFEEKKEEFIENLKKCMRLIFCEENLFVDYTADQKEYALLENEVGKFKPLLYRT
                     DPAEDGGRPVPEKKNEGFRT"
BASE COUNT          609 a          561 c          427 g          684 t
ORIGIN      
        1 ggtcctgaac ccttcattct ttttctccgg taccggcctg ccaccgtctt ctgcaggatc
       61 cgtgcggtat aaaagcggtt taaattttcc aacttcattt tcaagaagcg catattcctt
      121 ctgatcagcc gtataatcaa caaacagatt ctcctcacag aaaatcagac gcatgcattt
      181 tttcaggttt tcaataaatt cctctttttt ttcttcaaaa tgtgcttcca gaagctcgat
      241 cagccgataa aatgtcaccc cgccgatctg ttccgaaaca gaagctgttt ctgagcaata
      301 agacatggca cgcaaagccg cagtcgaatg tccggcactg accatcgttg tctgcagtct
      361 ggactttagc atcgccagaa tttcctttaa acgcttttca tctgaaaaat cagaagtaaa
      421 caaaatttcc tttatcattt caaatgcaaa cggcagctta tcaaacagta cttttgccct
      481 aacttcaaac atcgtccggt aattgtccgg atgtgcagat tccacataag taaccgcacc
      541 catagtaata ccgccggtat taatgttgat ttcattaaac agatccgcat acgtatagtt
      601 ctttgtactg acaaaaccga gcacattttt taatacgcca agatacggaa gcatcccggc
      661 cggaagcttt ccagtatcaa aaccaagtgt taaatatccg atcccatttg taaaaatatc
      721 atgtgtcagc aatatcgtcc ggtcaagccg gtgctccgca taaatatatg gctgtgcttc
      781 ttctttgatg tctgagatct taagcagcgg aatcgataac agctcttctt ttgtacttgg
      841 ctcctcctga tatgcgatca gtgcctttgt atctgcgatc agacgctctg tctcttcttc
      901 cgataacgac tctttatatg cctgcatttg atccgcaagt tttttgtcaa gctttgcggt
      961 cagcccgcgt tccggctcaa caaccacaac ggaagcatgc gtattgtgaa gcaggtatgt
     1021 ctcgaccagc ttttcaaaat atccgctccc tacatattct ctcattttag caaatgtttc
     1081 attggcttcg atatgaataa acggcagtgc atcatcatac agccagctgt caaatgcctg
     1141 cagtccgtac atcagtccct tcggataaga accaaaatcc gcttccctgt acttgaattc
     1201 aaaataattg ataccggcaa gcagtgcttt ttgatcgatt ccgttctgta caacattcga
     1261 cagtacttct tttatcacat ccagaaatgt atccagctgc tccgtattcg catattttgc
     1321 aatgacggaa aaatacggct gtaaaactcc gttttcatag gtactctgaa tatcctttcc
     1381 tatcccgcgg tctaacagcg tctgctttaa aacagcaccc ggtgcgagaa gaagcgcata
     1441 ttcgatgatc tgaaaggcca catacagttc tctgtccaga ctcgtaccga tcacggcatt
     1501 ataggagaga taggtattat cttcttccgt ctcctcatta gtgatcgagt attttttcgt
     1561 ttttctcaca ggctcatcaa acggcttctg cagctcgatt ttagaagata tcgcggcttc
     1621 ttcatatttt gacaaatatg cttcatcgat gaacgtcagt ttttccgcca tatcggcatt
     1681 accgtacaaa taaatgtagc tgttggacgg atgataatat ttccggtgaa aatcaagaaa
     1741 tttttcatag gtcagtgtcg ggatcacctc cggatccccg cctgattcat acgaatatgc
     1801 cgtatccgga aacagcgtat tgaatacttc gcggtccagc atgtcgtccg gtgaggaaaa
     1861 cgcacctttc atttcattat atacaacacc gttgatcgtg atctcatcgt ttttttcttc
     1921 aagctcataa tgccagcctt cctgcctgaa tatctgctct cttttataaa tattcggata
     1981 aaagaccgca tccagatata catgcatcag attctgaaaa tctttgtcat tacagcttgc
     2041 tacaggataa atcgtcttat ccggatatgt cattgcattc agaaatgtat taagagagcc
     2101 tttggcaagc tctacaaacg gatccttggc cggaaacagc tttgagccgc acagcacgga
     2161 atgctccagt atatggggaa gcccggtact gtcatacggc ggagtacgga acccgatgga
     2221 aaacactttg ttttcatcat cgtttgacag cagcagcact ctggctcctg attttttatg
     2281 c
//
LOCUS       DAOSYE010000352         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37842_length_2279_cov_2.512140, whole genome shotgun sequence.
ACCESSION   DAOSYE010000352 DAOSYE010000000
VERSION     DAOSYE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37842_length_2279_cov_2.512140"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLU43_06730"
     CDS             <1..267
                     /locus_tag="PLU43_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOO28143.1"
                     /translation="YCLVKDGEEVGSLPLRVGGLMSDMLPEQFIPALDQMLQKAADLG
                     VASGIDPFITLSFIALPVIPSVRLTDEGMFDVDRFCFFAEGDET"
     gene            284..1123
                     /gene="modD"
                     /locus_tag="PLU43_06735"
     CDS             284..1123
                     /gene="modD"
                     /locus_tag="PLU43_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HOO28144.1"
                     /translation="MYISEDYLDKLIAEDVPYFDLTTYVLGIEACAGTIEFFTREDAI
                     VCGTEEAERILKKLDVEVFERIPSGRKAAAGTVLLAGRGPAGSLHTGWKVCQNILDRY
                     TGIATKTNALVERAKAVNKDIAVVATRKIFPGTKALTVKAIVAGGAMPHRLGLSETVL
                     VFKQHMNFMGGFDGFLEKLPEIRHKICEKKLTVEAENMEQAMKLAKAGVDEIQFDKVP
                     AAEIRKGLSSLRAEKPDLLILAAGGINESNIEEYAASGADAIVTTSLFDSKPTDIGVR
                     IFA"
     gene            1366..>2279
                     /locus_tag="PLU43_06740"
     CDS             1366..>2279
                     /locus_tag="PLU43_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO28145.1"
                     /translation="MKKILSTLLAMGMAVSLLAGCGSTSTAESSDTSAAAESTEAAEE
                     STEETEAAEEPAADTAEEETEAASDVKIGVILVGDETEGYTKAHIDGITTAGQNVGLA
                     DSNILWKYNVPESDDCYAAAKELVANGCTLIISNSYGHQDYMAEAAEEFPDVSFVAMT
                     GDYAAISGLDNFYNAFTDVYESRYVSGVVAGMKIAELAEADLIPESGYDADHNVKIGY
                     VGAFPYAEVVSGYTAFFLGIKSVYPDVSMEVTYTNSWFDIEAEGAAAESLMADGCVVI
                     GQHADSTGAPTAVQTAQDKNGAVVYSVG"
BASE COUNT          666 a          415 c          626 g          572 t
ORIGIN      
        1 tactgtcttg taaaagacgg tgaagaggtc ggcagtctgc cgcttcgggt cggcggcctt
       61 atgagtgaca tgctgccgga gcagtttatt ccggcattgg accagatgct gcagaaagca
      121 gctgatcttg gagtggcatc cgggatcgat ccttttatta cattaagttt tattgcactg
      181 ccggtcattc cgtctgttcg tctgacagac gagggaatgt ttgatgtgga tcgattctgt
      241 ttttttgcag agggagatga aacctgaaga aagagggatt gcgatgtata tttcagaaga
      301 ttatctggac aaactgattg ctgaagatgt accgtatttt gatctgacga cctatgttct
      361 tggtatagag gcatgtgccg gtacgatcga atttttcaca cgggaagatg caatcgtgtg
      421 cggcactgag gaagcggaac ggatattaaa aaaactggat gttgaagttt ttgaaaggat
      481 accgtccgga cggaaggcag cggcagggac ggttcttctg gcgggcagag gccctgcggg
      541 aagccttcat acgggctgga aggtatgtca gaatattctt gaccgctata cggggatcgc
      601 tacaaaaaca aatgcgctgg ttgagagagc aaaggctgtc aataaggata ttgctgtggt
      661 tgctacgaga aaaatttttc cagggaccaa agcacttacg gtaaaggcca ttgttgcagg
      721 cggagcaatg ccgcacaggc ttggtttatc agaaacagtg cttgtattta aacagcatat
      781 gaattttatg ggagggtttg acggttttct tgagaagctg cctgagatcc ggcataaaat
      841 atgtgaaaag aagctgacgg ttgaagctga aaatatggag caggcaatga aacttgcaaa
      901 ggccggcgtg gatgagatcc agtttgataa agtaccggca gctgaaataa ggaaagggct
      961 tagttccctg agggcagaaa aaccagatct gttgattttg gcagcaggcg gtataaacga
     1021 aagcaacatc gaagaatatg cagcatccgg cgcggatgcc atagtgacga ccagcctgtt
     1081 tgattccaaa ccgaccgata ttggagtaag aatatttgca tgacaatgcg ggtatttttc
     1141 agaggtcaga tccatattac ccgaatggat tatgaccata taaaaataag aagatttttt
     1201 ggctgaaaaa taatatcagg cggaatttat ccttgacatt cggactaaaa tattatattc
     1261 tgagtgaaag tatcagatgc gaaggtgcac agagagatct gtacatcttt ttctgctttt
     1321 agccagaaaa acgcacaata ttaataaaat caggaggaag cgattatgaa aaagatttta
     1381 agtacattac ttgcaatggg tatggctgtt tccctgcttg caggatgcgg ttctaccagt
     1441 accgcggaaa gcagtgacac ttctgctgcg gcagaaagca cggaagccgc ggaagagagc
     1501 acagaggaga ccgaagcagc ggaagaacct gcagcggata cggcagaaga ggaaacagag
     1561 gctgcgtccg atgtgaagat cggtgttatt cttgtaggag atgagacaga aggctataca
     1621 aaagcccata tcgacggaat tacgacagca ggacagaatg taggtctggc agacagcaac
     1681 attctttgga agtataacgt accggagtca gatgactgtt atgcggcagc aaaggaactg
     1741 gttgcaaacg gatgtacatt gattatttca aacagttacg gacatcagga ttatatggca
     1801 gaggcagcgg aagaatttcc ggatgtaagc tttgtagcaa tgacaggtga ttatgctgca
     1861 atttccggtc ttgataattt ctataatgca tttacggatg tttacgaatc ccgttatgta
     1921 tccggtgttg ttgcaggtat gaagatcgca gagcttgcag aggcagatct gattcctgaa
     1981 tcaggctatg atgcagatca taatgtaaag atcggatatg ttggtgcatt cccgtatgca
     2041 gaagttgtat ccggctacac ggcatttttc cttggaatca aatccgtata tcctgacgta
     2101 tccatggaag taacctatac aaattcatgg ttcgacatcg aagcagaagg tgctgcggca
     2161 gaatcactga tggcagacgg ctgtgtagta atcggacagc atgccgattc cacaggtgct
     2221 cctacggcag tacagacagc gcaggataag aacggcgcag ttgtatattc ggtaggata
//
LOCUS       DAOSYE010000353         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37888_length_2277_cov_3.116112, whole genome shotgun sequence.
ACCESSION   DAOSYE010000353 DAOSYE010000000
VERSION     DAOSYE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37888_length_2277_cov_3.116112"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLU43_06745"
     CDS             <1..736
                     /locus_tag="PLU43_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO28146.1"
                     /translation="KRIRENADVSMERAQKGSTDIEQYTNQLSEVNQIMNQLSGVASE
                     LNTSTQEMNTIIESIANISSQTTLLSLNASIEAARAGQAGRGFAVVATEIKKLADDTQ
                     AAANRIGSIIEQVQGNVEEMTVKMQAGLTQLDKSNELARVTQGSFGDIREGTLVVNED
                     VQNIVSYMDEMSGIVEYVTKSVKGITDAIEQNVSMTTEISKTVESETESLQEVKESAG
                     NLETLAQELHAAVLEFKLKQQETEAP"
     gene            complement(739..1617)
                     /locus_tag="PLU43_06750"
     CDS             complement(739..1617)
                     /locus_tag="PLU43_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28147.1"
                     /translation="MNPAEFRKAYNDLNISFSLENYSISASNIVFEKFLRSIPMHSHS
                     SNSYEIHYIAYGKGMATIDGRDYEVGQNVLYITGPHIEHAQIPAPEDPMAEYCVYLRL
                     EEKTPCPKKDCNNQLVHTFVNRPFWFGQDSHNIHTIMQALFWELENKYPGYKIEAETL
                     LKQLLVAIVRNYSYTKETSSPLETQVLNDNQYLTIEECFLYEYNTLTLPELSDRLGLS
                     SRQTERLLQKHYGKTFLSKKTEAKMSAAVLLLRNPAKTITEISEELGYSSVEHFSSAF
                     KRYYHINARQYRKTKI"
     gene            1864..>2277
                     /locus_tag="PLU43_06755"
     CDS             1864..>2277
                     /locus_tag="PLU43_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO28148.1"
                     /translation="MAKMKGAILPGDSTVEVKEFEIPTPGYGQVLVKTKATTICGSDI
                     RCIYREHVGKGPEGYIPGTIAGHEPCGIIVEEGEGLKRFGKGDRVIVYHISGCGVCND
                     CRRGYYISCSSKHRKAYGWQRDGGMGEYILCDEKDL"
BASE COUNT          685 a          424 c          585 g          583 t
ORIGIN      
        1 aaagcgaatt cgtgaaaacg cggatgtgtc catggagcgt gcccagaaag gaagtactga
       61 tattgaacag tatacaaacc agctttccga ggtcaaccag atcatgaacc agctgtccgg
      121 tgtggcatct gagctgaata caagcacgca ggaaatgaac acgattattg aatcaattgc
      181 aaatatatca tcacaaacga cgctgttgtc tttgaatgct tccatagaag cagccagagc
      241 cggtcaggca ggaagaggat ttgccgttgt agcaacggaa atcaaaaagc ttgccgacga
      301 tacacaggca gctgcaaatc gtattggctc cattatcgag caggtgcagg gaaatgtcga
      361 agagatgaca gtaaagatgc aggcaggtct tacacagctt gataagagca atgagcttgc
      421 cagagtcacg cagggcagtt ttggtgatat cagggaagga acgcttgttg tcaatgaaga
      481 tgtacagaat attgtgtcat atatggatga aatgagcgga atcgtggaat atgtgacaaa
      541 gagcgtaaaa ggaatcacag atgcgatcga acagaatgta tcgatgacaa cagagatttc
      601 taaaaccgtg gaaagtgaaa ccgaaagtct gcaggaagta aaagaaagtg cgggcaacct
      661 ggaaacactg gcacaggaac tgcatgcggc cgtacttgag tttaaactga aacagcagga
      721 gacagaagca ccttaatatc agatctttgt tttcctgtac tgacgtgcat taatatgata
      781 atagcgtttg aacgcgctgg aaaaatgttc gacagaagaa taaccaagct cttcggaaat
      841 ttcagtaatg gtttttgcgg ggtttctaag taataagaca gcggccgaca ttttagcttc
      901 tgtctttttg cttaaaaaag tttttccata atgcttttgc agcagccgtt ccgtctgcct
      961 ggaacttagg ccgagccggt cggaaagctc gggaagggta agcgtattat attcatataa
     1021 aaaacattcc tcgatcgtca gatactggtt atcattcagc acctgggttt cgagaggaga
     1081 agaagtttct tttgtatagg agtaattgcg gacgattgcg acaagcagct gcttgagcag
     1141 tgtttccgct tcgatcttat agccgggata tttattttca agctcccaga ataatgcctg
     1201 catgatcgta tggatattgt ggctgtcctg tccgaaccag aaaggtctgt ttacaaaagt
     1261 atgtacgagc tgattgttac agtccttttt tggacaaggt gttttttctt ccagccgcag
     1321 atagacgcag tattctgcca ttgggtcctc aggggccggt atttgtgcat gttcaatgtg
     1381 cggaccggtg atgtacagga cgttttgtcc gacttcatag tcccttccgt cgatcgtagc
     1441 cattcctttt ccgtacgcga tgtagtggat ctcatagctg ttgctgctgt gactgtgcat
     1501 tggtatcgaa cgtaaaaatt tttcaaatac gatattggat gccgatatgg aatagttttc
     1561 taatgaaaaa gaaatattga ggtcgttata tgcttttctg aattccgctg gattcataag
     1621 atcagtcctt ctttgatccg gtccgtacga ccggctgtta taagaatact atacgatgtt
     1681 ctgcagataa ttacaatcag aacttgcgat aatgcgacat aaaaaagtta actatgtcag
     1741 ttgcttctgt aataaaatta tccggctgct gatataataa aaccaacaga taaacaaacc
     1801 gtcaaagcgg cggtaattac acggaaaaca gggcttttcc gacaagaaag gggttacttg
     1861 aaaatggcaa aaatgaaagg tgcgatctta ccgggcgaca gcacggtgga agtgaaagaa
     1921 tttgagatcc caacgccggg atatggacag gtacttgtaa aaacaaaggc gacaacgatc
     1981 tgcggcagtg atatccgctg tatttacagg gagcatgtcg gaaagggacc ggaaggatat
     2041 attccgggaa cgatcgcagg gcatgaaccg tgcggtatta tcgtggaaga gggagaaggc
     2101 ctgaagcggt ttgggaaggg tgacagagtg atcgtttatc atatttcagg ctgcggtgta
     2161 tgcaatgact gcagaagagg ctattatatt tcctgctcaa gcaaacacag aaaagcatat
     2221 ggctggcaga gagacggcgg aatgggagaa tacattctgt gtgatgaaaa agacctg
//
LOCUS       DAOSYE010000354         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_37935_length_2275_cov_3.254054, whole genome shotgun sequence.
ACCESSION   DAOSYE010000354 DAOSYE010000000
VERSION     DAOSYE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37935_length_2275_cov_3.254054"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLU43_06760"
     CDS             complement(<1..331)
                     /locus_tag="PLU43_06760"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949067.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO28149.1"
                     /translation="MADKKLVEAITSMEEDFAQWYTDVVKKAELIDYSNVKGCMVIKP
                     AGYAIWELIQRQLDERFKQTGVENVYMPLFIPESLLQKEKDHVEGFAPEVAWVTHGGL
                     EPLPERMC"
     gene            complement(399..524)
                     /locus_tag="PLU43_06765"
     CDS             complement(399..524)
                     /locus_tag="PLU43_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28150.1"
                     /translation="MLKIPAQGSGTCLGVLYENTAYGGVNAAGESAQMLKGVVPP"
     gene            complement(608..1303)
                     /locus_tag="PLU43_06770"
     CDS             complement(608..1303)
                     /locus_tag="PLU43_06770"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO28151.1"
                     /translation="MDILFEDKYLLVCRKPAGMPTQTAGVCAKDMVSEVKNYLKTKEK
                     GDLYTGLVHRLDQPVEGVLVFAKNSFAAANLSRQLHEGNMKKYYDAAVCGIVCDQEEV
                     KLTHYLVKDAKAKKALFADEKTPGAKRAELLYKVTVTDEEAGESLLRIRLLTGRFHQI
                     RAQLAKIGHPIVNDTKYGGRKEEGSGNSRSIALCACCLEFVHPKTNKGMRFEIEPCQE
                     DLKRLNSLTNPCK"
     gene            complement(1305..1841)
                     /locus_tag="PLU43_06775"
     CDS             complement(1305..1841)
                     /locus_tag="PLU43_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOO28152.1"
                     /translation="MSKYAGTKTEKNLMDAFAGESQARNKYTFYASAAKKAGYEQLAA
                     LYLETADQEKEHAKMWFKEFHGIASEEVNLEDAAAGENYEWTDMYARMAREAREEGFP
                     VLAAKFEGVAKVEATHEKRYLKLLDSMKNNKTFKGDAPLGWKCRNCGYVHEGESAPEI
                     CPVCAHPKAYFERKVENY"
     gene            complement(1860..>2275)
                     /locus_tag="PLU43_06780"
     CDS             complement(1860..>2275)
                     /locus_tag="PLU43_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOO28153.1"
                     /translation="EHVVGVAKGVSQDIIDDLRANFTGECSEVGMYLAMSRVAHREGY
                     PEIGLYWEKAAHEEAEHAAKFAEMLGEVLTDSTKKNLEMRVEAENGACAGKFDLAKRA
                     KAANLDAIHDTVHEMARDEARHGKAFEGLLKRYFQ"
BASE COUNT          504 a          658 c          394 g          719 t
ORIGIN      
        1 cgcacatccg ttccggaagc ggctccaaac cgccgtgtgt gacccatgca acttcaggtg
       61 caaacccttc cacatggtcc ttctccttct gcaaaaggct ttccggaata aacagcggca
      121 tataaacatt ttccacaccg gtctgtttaa atctttcatc aagctgtctc tggatcagtt
      181 cccagatcgc atagcccgcc ggtttaatga ccatacagcc cttcacattg gaataatcga
      241 tcagttccgc ctttttcaca acatccgtgt accattgcgc aaaatcctct tccatcgagg
      301 taatcgcttc tacaagtttc ttgtcagcca tactttttct cctttaatat ccatcatttt
      361 ccgataagaa aaataagccc accaatgcag aggacccatt acggcggtac cacccctttt
      421 agcatttgtg cgctctctcc tgcagcgtta acgccgccgt acgctgtatt ttcatacagg
      481 actccaaggc aggttccgga tccctgcgca ggaattttca gcatccattc cctctctgcg
      541 gcgcatcggt atcattctca tccccgtact aacccttttc tcagtctttg tctatcgtca
      601 ttctatttta tttgcaggga tttgtcaagg aattcaatcg ttttaaatcc tcctgacacg
      661 gctctatttc aaaccgcatt cccttatttg tctttggatg gacaaactcc agacagcagg
      721 cacataatgc aatactgcgg gaattcccgc tgccttcctc tttccgtccg ccgtattttg
      781 tatcattgac gatcggatgc ccgatctttg ccagctgtgc cctgatctga tggaaccgcc
      841 cggtcagcag acggatcctc agcaggcttt cccctgcttc ttcatctgtc accgttactt
      901 tgtagagcag ctccgccctt ttcgctcccg gcgttttttc atcagcaaac agtgctttct
      961 tcgcttttgc atccttcacc agatagtgcg tcagttttac ctcttcctga tcgcatacga
     1021 ttccacatac tgcggcatca taatactttt tcatatttcc ttcatggagc tgcctgctta
     1081 aatttgcagc tgcaaaagaa tttttcgcaa aaacaaggac accctcaaca ggctggtcca
     1141 aacgatggac cagccctgta tataaatcgc ctttttcttt tgtttttaaa taattcttta
     1201 cttcactgac catatccttt gcgcagacac cggcggtctg cgtaggcatc cccgccggtt
     1261 ttctgcatac aagcagatat ttgtcttcaa ataaaatatc catatcagta gttttctacc
     1321 ttacgctcaa aatatgcctt tggatgtgca cagaccggac agatctccgg tgccgactcg
     1381 ccttcatgca catatccgca gttacggcac ttccagccaa gaggagcgtc tcccttaaat
     1441 gttttattat tcttcatact gtcaagaagc ttcagatatc gcttttcatg tgtcgcctcg
     1501 acctttgcaa cgccttcaaa cttcgctgcc agtaccggaa agccttcttc tcttgcttct
     1561 ctggccatac gcgcatacat atccgtccat tcgtaattct cgcctgctgc cgcatcctca
     1621 aggtttactt cttcactggc gattccgtga aattccttaa accacatttt agcatgctct
     1681 ttttcctggt ctgctgtttc cagatacaga gccgcaagct gctcatagcc tgcttttttt
     1741 gctgccgacg cataaaacgt atatttattt cttgcctgtg attcacctgc aaaagcatcc
     1801 attaaattct tttccgtttt tgttcctgca tattttgaca tagtcctacc tccctgtttt
     1861 tattgaaaat aacgttttaa cagtccttcg aatgcttttc catggcgagc ctcgtcacgg
     1921 gccatttcat gaacagtatc atggatcgcg tcaagatttg ctgctttggc acgttttgca
     1981 agatcaaact tgcctgcaca tgcgccgttt tccgcttcca ctctcatctc aagattcttc
     2041 tttgtgctgt ctgtcaagac ctcaccaagc atctcggcaa atttagccgc atgttctgct
     2101 tcttcatgtg ctgctttttc ccaatataac ccgatctccg gatatccctc tctgtgtgca
     2161 actcttgaca ttgcaagata catgccgact tcagaacatt ctccggtaaa attcgccctt
     2221 aaatcatcaa taatatcctg gctgacacct tttgcaactc ctaccacatg ttctg
//
LOCUS       DAOSYE010000355         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_38035_length_2271_cov_1.888087, whole genome shotgun sequence.
ACCESSION   DAOSYE010000355 DAOSYE010000000
VERSION     DAOSYE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38035_length_2271_cov_1.888087"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1084
                     /locus_tag="PLU43_06785"
     CDS             44..1084
                     /locus_tag="PLU43_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28154.1"
                     /translation="MKKFLFKILVFFLILFAIFAPVNILIDPYNIFHYDDPVNNGVEP
                     NKNFIKTKYIIHNQDKFDSLVFGSSRAGFIDVEKIPGGTYYDMASSEAVPQEHVNTLK
                     VLIKNGFVPKNILLLVDDISCFVDPAVHENTLYRVPYPTGGFISQLEFYSKYCDLLTT
                     YQSLSVIREHEDNDPDFAERFRRTGTERLDIVSEFTGKYEDGTEVPGYWADYYSLRLT
                     EATAQIQEFVDICEQYDINLTIVTNPLYCKTYERDLENGYLEFLDALADVTDYYNFSS
                     FSNVTEGTGNYYEASHFIPAIGRMMIDAAYNGEVDPVLLKQGFGVKVTAENKEEFIEF
                     LREQAVENGVGL"
     gene            1081..1863
                     /locus_tag="PLU43_06790"
     CDS             1081..1863
                     /locus_tag="PLU43_06790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO28155.1"
                     /translation="MKKSFGILICILPYFAMLLIRMTGAAVFGRIFETGLGMDYNTYV
                     VYVLALIAVIYIIVFGVWYYFGFARKNQGEGQDSFWTIRTMLLLLLLGAAVQIAVSYI
                     LFFLLSLSPSLSDEYMSMLSPLLSLSPAAVLYAGILSPVGEECIFRGVILGYAKENTR
                     FYLANFLQAALFGVFHGNLVQGIYAFLLGLLLGYLVKLSGSVFGGIIFHSALNFAGLY
                     LERLLPEELPVYLKVFFMLAAAAVCALILFVLSRHGKNKNRA"
     gene            complement(1860..>2271)
                     /locus_tag="PLU43_06795"
     CDS             complement(1860..>2271)
                     /locus_tag="PLU43_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28156.1"
                     /translation="HELTLIRIDYGFLPKLLFQRCADVIGKDLRIYVLGNKMLAAMMR
                     ISSGSDFRANFSLGGHAIPHTLTNEETALAKKIITALPADFVGIDFLYHKDHPVLSEI
                     EDVVGTRMLYQHTNIHAVREYLNYILLSMQNTTA"
BASE COUNT          572 a          393 c          556 g          750 t
ORIGIN      
        1 aatttatata ttttaagttc taacgatatg aaagggatca tgtatgaaaa agtttttatt
       61 taaaatactt gtatttttct tgatattatt tgcgattttt gcaccggtaa atatattgat
      121 tgatccatat aatatttttc attatgatga tccggtgaat aatggcgttg agcccaataa
      181 gaattttatt aaaacaaaat acataattca taatcaggat aagtttgatt cccttgtatt
      241 tggctcttcg cgtgcgggat ttatcgatgt cgaaaagatt cccgggggaa cctattatga
      301 catggccagt tcggaggctg ttccacagga gcatgtgaat acgctgaagg tattgatcaa
      361 aaatggattt gttccgaaga atatcctgct tcttgtagat gatatttcct gttttgtgga
      421 tcctgcggtt cacgaaaata cactttaccg ggtgccttat ccaacaggag ggttcatttc
      481 ccagcttgaa ttttattcaa agtactgcga tcttctgacg acatatcagt cgttgtcggt
      541 tattagggaa catgaggaca acgatcctga ttttgcggag cgttttagaa gaacaggaac
      601 cgagcgtctg gatattgtat ctgaatttac cggaaagtat gaagacggaa cggaggttcc
      661 gggctattgg gcagattact attccctgcg tctgactgaa gcaactgcgc aaatacagga
      721 atttgtagat atttgtgaac aatatgatat caacctgaca attgtgacaa atcctcttta
      781 ctgtaagacc tatgaaaggg atcttgaaaa tgggtatctg gagtttcttg atgcgctggc
      841 tgatgtgacg gattattata atttcagcag tttttccaat gttacggaag gaaccgggaa
      901 ttattatgaa gccagtcatt ttattccggc gatcggtcgt atgatgatcg atgccgcgta
      961 taacggcgag gtagatcctg tgcttctgaa acagggcttt ggcgtgaaag ttactgcgga
     1021 aaataaagag gagttcatag aatttctgag agaacaggca gttgaaaatg gagtggggtt
     1081 atgaaaaagt cttttggtat actgatctgt attttgccat attttgcaat gcttttgatc
     1141 cgcatgaccg gggctgcggt attcgggcgg atctttgaaa ccggacttgg aatggattac
     1201 aatacatatg tcgtctatgt acttgcattg attgcggtta tttatatcat tgtgttcggc
     1261 gtatggtatt attttggttt tgcgagaaaa aatcagggag aaggacagga ttctttttgg
     1321 actattcgta cgatgctgtt gctgcttttg cttggagctg cggttcagat agcagtttcg
     1381 tatattcttt tttttctgct gtccctgtcg ccttcgcttt ccgatgaata tatgtcgatg
     1441 ctttcacctc ttctttcttt gtccccggcc gcagtgctgt atgccggtat tttgtcgcct
     1501 gtcggagagg aatgcatttt tcgcggcgtg attttaggct atgcaaagga aaatacacgg
     1561 ttttatctgg ctaatttttt gcaggcagct ttattcggtg ttttccatgg aaatcttgta
     1621 cagggaatct atgcgttttt gctcggtctt ttactgggtt atcttgtgaa gctgtcaggt
     1681 tcggttttcg gcggaattat ttttcacagt gcgcttaatt ttgccggtct gtatttggaa
     1741 cggctgctgc cggaagaact gccggtctat ttgaaggtgt ttttcatgct tgctgctgcc
     1801 gccgtttgtg cgttgatcct gtttgtactt agccggcacg gtaaaaataa aaatagggct
     1861 taggcagtgg tgttctgcat ggaaagcagg atataattca gatattccct gactgcgtgt
     1921 atgtttgtat gttgataaag catacgtgtt ccgacgacat cttcgatctc actgagaaca
     1981 ggatggtctt tatggtagag gaagtcgatg ccgacgaaat cggccggcag tgctgtaatg
     2041 attttttttg caagggctgt ttcttcattt gtaagagtat gcgggatggc atggccgcca
     2101 agagaaaagt tggcgcgaaa atcggaaccg gagctgatcc gcatcattgc ggcaagcatt
     2161 ttattgccaa gaacgtaaat acgcagatct tttccaatca catcggcgca gcgctgaaac
     2221 agcagttttg gtaaaaatcc ataatctatt cttataagag ttagttcatg a
//
LOCUS       DAOSYE010000356         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_38545_length_2252_cov_3.022758, whole genome shotgun sequence.
ACCESSION   DAOSYE010000356 DAOSYE010000000
VERSION     DAOSYE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38545_length_2252_cov_3.022758"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLU43_06800"
     CDS             complement(<1..323)
                     /locus_tag="PLU43_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28157.1"
                     /translation="MKKKTGIARLLEIAGERRGLLILSGLLSVLSALIQMVPFAAIYF
                     IAKELLQNAASPEFIDKNLIRNWGVYALISLAVALVLLYAGTMASHVSAFRIFLNPKS
                     IPLMNS"
     gene            complement(341..955)
                     /locus_tag="PLU43_06805"
     CDS             complement(341..955)
                     /locus_tag="PLU43_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HOO28158.1"
                     /translation="MKKTNTQKLMGKDFVTIGIFSILLMAVFIVVSLPFMPFLSVAYP
                     LIGVASAIFTAPVFMLMTYKVAKRGTVFLCSTIISLLYVIMGYVYLLPFGIAAGMLCE
                     AIVWKKGAYRSFWYNSASFSVFCVWLYLGSSFLPIYIFGTEYYKKLLSGNEESALVHI
                     KFATSPLWAAGSVIITVIAAVIGCLIGRRLLKKHFIKAGVIAAE"
     gene            complement(1065..2072)
                     /locus_tag="PLU43_06810"
     CDS             complement(1065..2072)
                     /locus_tag="PLU43_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28159.1"
                     /translation="MTDEKIEYPVIVNPNNQNCQMCAGFNSLYHTDSFESKRCLPEMI
                     GDGYCKRITIKPSVEISIVDMTFFEDIVIDGRGEQTPSFYDLSFCLEGSFRWKTKGRK
                     LELETQGGKSFLLCGEDVYGLSKIHEGQSFSYVNIRMQESFIKKLLSGAGAGDAAAAK
                     FCSFEFLKQGEIGFSMKKILNEIINCRLSQNMKKIYLEAKIMELFAVYMEETIFENSA
                     EPSSTDFSKEDLAGLRKAKEILDSDIVNVPSLSRLARMTHLNEYKLKRGFKALFGQPV
                     HSYVIDKRLEGAYGLLESGTRSVSETAQLVGYNDLGRFAEKFRKKFGVNPSEYKKYNQ
                     V"
BASE COUNT          660 a          553 c          391 g          648 t
ORIGIN      
        1 gaattcatta aaggtatgga tttagggttc aaaaatatcc gaaaagcgga aacatgggag
       61 gccatcgtgc ctgcataaag aagaacaagt gccacggcaa gagaaataag cgcgtacacc
      121 ccccagttcc tgattagatt tttgtcgata aattccggcg atgccgcatt ttgcagaagc
      181 tccttcgcaa taaaatagat tgctgcaaaa ggaaccatct gtataagcgc gcttaaaacg
      241 gataaaagac cggataaaat taaaagtccc cgtcgttccc ctgcaatctc cagcagtctt
      301 gcaattcctg tttttttctt caaaatttca cctcatcccg ttattcagcc gctatcacgc
      361 ctgcttttat aaaatgtttt ttcagcagcc tacgcccgat cagacagccg ataactgctg
      421 caattacggt tatgatcact gagccggccg cccataacgg cgacgtcgca aattttatat
      481 gtaccagtgc cgattcttca ttcccggaaa gcagcttttt ataatattcc gttccgaaaa
      541 tatagattgg caggaatgag gatccaagat acagccatac acaaaaaaca gaaaagctcg
      601 cgctattata ccagaagctg cggtaagcac ccttcttcca tacgattgct tcgcatagca
      661 ttcctgcagc aattccaaac ggaagcaaat aaacatatcc catgattaca tagagcagac
      721 tgatgatcgt actgcacaaa aatacggtcc cacgctttgc gaccttatag gtcatcagca
      781 taaagaccgg tgcggtaaag atcgcactcg ccactccgat cagcggatag gccactgata
      841 gaaaaggcat aaacggcaga cttactacaa taaaaaccgc cataagcaat atactgaaaa
      901 tcccgattgt tacaaaatct tttcccatta atttctgcgt atttgttttc ttcataataa
      961 aaaactcctt tcagacgact tcaatattgt attttagtcg ttccaaatac accatgctat
     1021 ggaaaaaggg attattttta cgcaaaaggg gattccctga ctgtttacac ctgattatat
     1081 tttttgtact ccgaaggatt tacaccaaat ttctttctga atttttccgc aaaacgcccc
     1141 agatcattat atcctacaag ctgggctgtc tccgaaacgc tccgtgtccc gctctctaaa
     1201 agaccatacg caccttccag tcttttatcg atcacatagg aatggaccgg ctgtccgaaa
     1261 agcgctttga atccccgctt cagcttatat tcgttcagat gtgtcatccg tgcaagccgg
     1321 cttaaagacg gaacatttac gatatccgaa tcaaggatct ctttcgcctt tctaagaccg
     1381 gccagatctt ctttggaaaa atcggtagaa gacggctccg cactgttttc aaatatcgtc
     1441 tcctccatat aaacagcaaa caattccatg atttttgctt caagatatat ttttttcata
     1501 ttttgtgaca atcggcagtt tatgatctca tttaatattt ttttcatgga aaacccgatc
     1561 tctccctgct ttagaaactc aaaagagcaa aactttgcag ctgccgcatc tcctgcccct
     1621 gcgccgctta ataacttttt gataaaggat tcctgcatcc ggatattgac atacgaaaag
     1681 ctttgtcctt catgaatctt gcttaatcca taaacgtctt ccccgcaaag cagaaaactc
     1741 tttcctccct gcgtttcaag ttcaagcttt cttccttttg ttttccatcg aaagctccct
     1801 tccaggcaaa aagacaggtc ataaaaagag ggggtctgct cccctcttcc atcaattacg
     1861 atatcttcaa aaaacgtcat atccacaata gagatctcca ccgaaggctt tattgtgatc
     1921 cttttacaat aaccgtctcc gatcatttcg ggcagacatc gtttactctc aaaagaatcc
     1981 gtatgataca gactgttaaa tccggcgcac atctggcaat tctgattatt tggattaacg
     2041 atcacaggat actcgatctt ttcgtccgtc attttctatc catcctttcc atagattttc
     2101 aactgtgttt cttattttta tgtttcgtcg cgacaacata agaaagagga aaggatgatt
     2161 caaacaaagc tgaatccaca atcgtaaatc cgttctccct gagaaattca tggtagcttt
     2221 gttccgtcca tttatgataa gcccggaatc cg
//
LOCUS       DAOSYE010000357         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_38683_length_2247_cov_2.335310, whole genome shotgun sequence.
ACCESSION   DAOSYE010000357 DAOSYE010000000
VERSION     DAOSYE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38683_length_2247_cov_2.335310"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLU43_06815"
     CDS             <1..252
                     /locus_tag="PLU43_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOO28160.1"
                     /translation="ALKNSEVLIHQPSISGGLQGQATDIKIHSDWLEKTKEKLNEIYH
                     RLTEQPLEKIKADMERDYYMTAEQAKAYGLIDGILVSRG"
     gene            462..1598
                     /locus_tag="PLU43_06820"
     CDS             462..1598
                     /locus_tag="PLU43_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOO28161.1"
                     /translation="MKFIHLSDLHIGKRINDFSMLEDQEYILKRIISIIDEEMPDGVL
                     IAGDIYDKSVPSAESVQLFDGFLVQLSKRDLQVFVISGNHDSPERIAFGSRLMSSSGV
                     YMSPVYDGAVVPVSLSDKYGMVNVFMLPFIKPSHVKRFYPDEDVLTYSDAVQVAIKQM
                     TVDPKDRNLLITHQFVTGASRCDSEDIAVGGSDNVDVSVFTAFDYVALGHIHSPQNVG
                     SEAVRYCGTPLKYSFSEAGQSKSVTVVELKTKGCMTVRTVLLVPKRDMVEIKGSYMEI
                     TSRDFYKDMDTAAYMHITLTDEEDIPDAVGKLRAIYPNIMKLDYDNKRTRSNLEMYGV
                     DDVENRTPVELFTEFYEKQNNQPLSAQQRAFVTGVIEKIWEGAQ"
     gene            1595..>2247
                     /locus_tag="PLU43_06825"
     CDS             1595..>2247
                     /locus_tag="PLU43_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOO28162.1"
                     /translation="MRPIKLTLSAFGPYARKAELNMNQLGDRGLYLITGNTGAGKTTI
                     FDAITFALYGEASGENRDANMLRSKYADPDTPTFVELYFLYAGKTYHVTRNPEYERPS
                     KRGMGMTIQKADAELVYPDGRIITKTRDVSSAIQEVIGIDRGQFTQIAMIAQGDFLKL
                     LLAPTEDRKKIFRQIFKTGLYQDLQDKLKEESGRLGKDHEILKTSIQQYVNGTFCNE"
BASE COUNT          675 a          419 c          538 g          615 t
ORIGIN      
        1 gcactgaaaa acagcgaagt actgattcat cagccttcga tatccggagg attacagggg
       61 caggccacag acataaaaat ccatagcgac tggcttgaaa agacaaaaga aaagctaaat
      121 gaaatatatc acaggcttac ggaacagcct cttgaaaaaa tcaaggctga tatggaaaga
      181 gactattata tgacagccga acaggcaaaa gcatacggat taattgacgg gatcctggtg
      241 agccgcggat agtaatgcat ggcagcacta ttcaattcag tcaggttata agctatattt
      301 ttgttggtaa caaaccaatg agaatgtagc ttttttgatt gataatactg tgaatgcttc
      361 cataatttgt acaaaacaaa ttgctttatt tattgcagcg acatataata tacataatgt
      421 taaaaattta tctgtgttta gcacaaactg aagggaaaat tatgaaattt attcatttgt
      481 ctgatcttca tataggaaag cgcatcaatg atttttcaat gctggaagat caggagtata
      541 tattgaaaag gatcatcagc atcatcgatg aagagatgcc tgacggcgtg ctgattgccg
      601 gtgatatcta tgacaaatcc gtaccgtcgg cagagtccgt gcaattattt gatggatttc
      661 ttgtacagct ctccaaacgg gatctgcagg tgttcgttat aagcggaaat catgattcgc
      721 cggagcggat tgcttttgga tcaaggctga tgagcagcag cggagtttat atgtcgcctg
      781 tttatgacgg agctgttgtg cctgtcagcc tgtctgacaa gtatggtatg gtaaatgttt
      841 ttatgcttcc gtttataaaa ccttcccacg tgaaaagatt ctatccggat gaagatgttt
      901 tgacttattc agatgccgtc caggtcgcca tcaaacaaat gacggttgat ccaaaagacc
      961 ggaatcttct tattacacat caatttgtta caggtgcttc ccgctgtgat tcggaagaca
     1021 ttgctgtcgg cggttccgat aatgttgacg tgagtgtgtt tacagcgttt gattatgttg
     1081 cactggggca tattcatagt ccgcagaatg ttggctctga agctgtccgg tattgcggaa
     1141 caccgctgaa atattcgttt tctgaagccg gccagagcaa gtctgttacg gttgttgaac
     1201 tgaaaacaaa aggatgtatg actgttcgta cagttttgct tgttccaaag agggatatgg
     1261 tggagatcaa aggaagttac atggaaatta cgtcccggga tttttataag gatatggata
     1321 cggcagccta tatgcatatt acgctgacag atgaagaaga tattcctgat gcggtcggaa
     1381 agctccgggc aatttatcct aatattatga agctggatta tgataacaaa cgtacccgtt
     1441 ccaatctgga gatgtatggt gtggacgatg tggagaacag gactccggtc gaattattca
     1501 cagaatttta tgaaaagcag aacaatcagc ctctttctgc acagcagcgg gcttttgtaa
     1561 ccggagtgat tgaaaagata tgggagggcg cacaatgaga ccgatcaaac tgacgctttc
     1621 tgcttttggt ccttatgcca gaaaggctga actgaatatg aaccagcttg gagaccgggg
     1681 gctgtatctg atcacaggca ataccggcgc aggcaagaca accatttttg atgcgatcac
     1741 ctttgccctt tatggtgaag ccagcggtga aaatcgtgat gccaatatgc tgcgcagtaa
     1801 atatgctgat ccggacacgc ctactttcgt ggaactttat tttttgtatg caggaaaaac
     1861 ctatcatgtc actcgtaatc cggaatatga acgtccgtcc aaacgcggca tgggtatgac
     1921 gatacaaaaa gcagatgcgg aactcgttta tcctgatggc agaatcatta ctaaaacacg
     1981 ggatgtatcg agtgcaattc aggaagtcat tggtattgac cgcggtcagt ttacccagat
     2041 cgcaatgatt gcacagggag acttcctgaa gcttttgctt gcaccaacgg aggatcgtaa
     2101 aaagatattt cgtcagatat ttaaaaccgg actgtatcag gaccttcagg acaaattgaa
     2161 agaagagtcc ggcagacttg gaaaagatca tgaaattttg aagaccagta ttcagcaata
     2221 tgtaaatgga acgttctgca atgaaga
//
LOCUS       DAOSYE010000358         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_38948_length_2237_cov_2.621448, whole genome shotgun sequence.
ACCESSION   DAOSYE010000358 DAOSYE010000000
VERSION     DAOSYE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38948_length_2237_cov_2.621448"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLU43_06830"
     CDS             <1..242
                     /locus_tag="PLU43_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOO28163.1"
                     /translation="VTVLFEEDGQEAEYKIVTTVRSNSLKGLISIESPLGKALLGHKN
                     GDRISVQVNENISYHVIIKAIDKSTGCADDAIRQY"
     gene            412..930
                     /locus_tag="PLU43_06835"
     CDS             412..930
                     /locus_tag="PLU43_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOO28164.1"
                     /translation="MHYLVISTLPRNYEENRQVLSELAMEGITASVMYEEDYHIEGCK
                     GCTFCWLRTPGRCVVKDDYEILLREMLKADVVIFLSEAKLGFVSYRLKNIIDRIIPMV
                     EPYVVIRNGECRHIPRYEKRPEFALVYTGEKNGDFLNQWLACVTKNMNSTSIGAYHYE
                     ERQVFAHAVNNI"
     gene            911..1582
                     /locus_tag="PLU43_06840"
     CDS             911..1582
                     /locus_tag="PLU43_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28165.1"
                     /translation="MQLTIFSCSPQVNEKSASAFIAQAFKIGFTSDHKNTADIYYLRE
                     HSQWEMYREKFAAGSRILFVLPLYVDCIPGLLMEFFEYLAGWETLESAALDKQLAFIV
                     QSGFPEGLQLRTCELFLEKLPSHLGCEYAGTLVKGGLYGIAYAFGTRAKEQAGNAFMD
                     WGKRFAKELSFEKEAADRFAAPEKIEGIKLWTLKLMLPFSRLMWNRLARKTGVSGRLD
                     EKPYA"
     gene            1777..>2237
                     /locus_tag="PLU43_06845"
     CDS             1777..>2237
                     /locus_tag="PLU43_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HOO28166.1"
                     /translation="MALLEKWHEKAYSQTANKGDLQKLWAAYFEKEKEIYKKLLKKLD
                     EPVTGTVEALAQEYKVDLMTMTGFLDGINDSLIEPNPIEEMESETEVSLAFDKELLYK
                     NMVAAQADWLYGLEEWHEIYDEETLQRLYKEQKRSTTIVKGEKIGRNDPCP"
BASE COUNT          694 a          382 c          575 g          586 t
ORIGIN      
        1 cggtcacggt attgtttgaa gaagacgggc aggaggcgga atataaaatt gtgacgacgg
       61 tgcgcagcaa ttccctgaaa ggcctgatca gcattgaatc accgcttgga aaagcactgc
      121 tcggacataa gaacggtgac cgcatatctg tgcaggtcaa tgaaaatatc agttatcatg
      181 ttataataaa ggcgatcgat aaatcaacag gctgcgcgga tgatgccatt cgccagtatt
      241 aagctgtctg tttcagacgt ctggaataaa tgtcatgact tcatatgaca attgtaataa
      301 atatccataa agctattgaa atcagaaacc ttatcctgta aggtaaaaac aagcaggatg
      361 ggtttcgttt ttttatactg cgttatctgt aaaagaggag gtcatggaaa catgcattat
      421 ttggtaatat ccacattgcc ccgcaattat gaggaaaaca gacaggtgct gtctgaatta
      481 gccatggaag gaattaccgc ttcagttatg tatgaagagg attatcatat cgaaggctgc
      541 aagggctgca ctttttgctg gttaagaaca cccggcagat gtgttgtgaa agatgattat
      601 gaaatactgc ttcgggaaat gctgaaagcg gatgttgtta tatttttgtc cgaggcaaag
      661 cttggatttg tatcctaccg tctgaaaaat atcattgacc gtatcattcc tatggtagaa
      721 ccctatgtgg tgatcagaaa cggagaatgc aggcatattc caaggtatga aaaaagaccg
      781 gagtttgcgc ttgtttatac cggtgaaaaa aacggagatt ttttgaatca atggcttgcg
      841 tgcgttacaa aaaatatgaa cagtacttcg atcggtgcat atcattatga agaaaggcag
      901 gtatttgcac atgcagttaa caatatttag ctgttctccg caggtgaacg agaagagtgc
      961 ttccgctttt atagcgcagg catttaaaat cgggtttacg tcagatcata aaaatacggc
     1021 agatatttat tatctgcggg agcacagtca gtgggaaatg tacagggaaa aatttgcggc
     1081 cggcagcagg atcctttttg tccttccgct ttatgtggat tgcattcccg gacttctgat
     1141 ggagtttttt gaatatcttg caggctggga gacacttgag agcgctgcct tggataaaca
     1201 gctggcgttt atcgttcagt ccggttttcc ggaagggctg cagctgcgta cctgtgaact
     1261 gtttttggaa aagctgcctt ctcatcttgg atgtgaatac gcaggcaccc tggtgaaagg
     1321 cggtctgtat ggaattgcct atgcatttgg cacaagggca aaggaacagg caggaaatgc
     1381 ttttatggat tggggaaagc ggtttgcaaa agaacttagc tttgaaaagg aggcagccga
     1441 ccggttcgct gcgccagaga aaattgaagg aataaaattg tggacattga agctgatgct
     1501 tccgttcagc cgtcttatgt ggaacagact ggcaaggaaa accggagtaa gcggaaggct
     1561 tgatgaaaaa ccttatgcat aagctgcaga atatataaaa aagacgcaac gatgttgcgt
     1621 ttttttgtta ttttgtgtaa aatcagtatg taaagattac aacaaaaaag ttgcaaagtt
     1681 atgttgatgc ttgtatacta tagtttattg agcgaaacca tgcagcatcg gactgccggg
     1741 ttcaaattcc gtaagagtaa agagggagac gttaatatgg cactattgga aaaatggcat
     1801 gaaaaggcat attcacagac agcaaataaa ggtgatctgc aaaaactgtg ggcggcatat
     1861 tttgaaaagg aaaaagaaat atataaaaag ctgttaaaga agctggatga gccggtaaca
     1921 ggcacggttg aggctcttgc ccaggaatat aaagtggatc tgatgacgat gaccggtttt
     1981 ctggacggaa tcaatgacag tctgatcgag cccaacccga tcgaagagat ggaatcagag
     2041 acagaagtca gtctggcatt tgacaaagag cttttatata aaaatatggt tgcggcacag
     2101 gcggactggc tgtatggact tgaggaatgg catgaaattt atgacgagga gacccttcag
     2161 agactgtata aagagcagaa gcgttctact acgatcgtaa aaggtgaaaa aatagggcgt
     2221 aacgacccat gtccgtg
//
LOCUS       DAOSYE010000359         2230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39129_length_2230_cov_2.447356, whole genome shotgun sequence.
ACCESSION   DAOSYE010000359 DAOSYE010000000
VERSION     DAOSYE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39129_length_2230_cov_2.447356"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..424
                     /locus_tag="PLU43_06850"
     CDS             44..424
                     /locus_tag="PLU43_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO28167.1"
                     /translation="MIVEIDFNSEEAIYIQLRNQIVIGIATSQLKEGDTLPSVRQLAE
                     LAGINMHTVNKAYAVLRDEGFVKIDRRKGAVIALDINKLKAMEEIKEELLILLAKASL
                     KNITAAEIHEMINEIYAEYGCTIE"
     gene            459..>2230
                     /locus_tag="PLU43_06855"
     CDS             459..>2230
                     /locus_tag="PLU43_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOO28168.1"
                     /translation="MALKYTDKAEKALELAGRISRELKNGYIGTEHLLLGLIKEETGV
                     AAKVLTGNGISAESIVELIRQQIAFTSPANIRERDGWTPKAEAIIEKSGDEAFRFRAE
                     RIGTEHLLLALLNDNSSLAVRLLNTLGVNLQKLHVDILLAMGEDVAKYKEELQNAKLG
                     KKKNQGSGLLVDQYSRDLTALATEGKLDPVIGREMEISRIIQTLSRRTKNNPCLIGEP
                     GVGKTAIVEGLARRIVEGLVPETVQNKRVLTLDLSGMVAGSKYRGEFEERIKRVVKEV
                     TEAGNILLFLDEMHTIIGAGGAEGAIDASNILKPSLARGEIQLIGATTYEEYHKYVQK
                     DAALERRFQPIMVEEPSEEITVQILDGIKNNYEKHHGVEITEEAVAAAVQMSVRYIND
                     RFLPDKAIDLMDEAASKVRLKGLKAPAGLEELEQQLQNFEERKEQLLKKDLIEECGEL
                     RKEEEAVRKVYEKKKKRFENSMKGKKPVVDADEIADVVSEWTKIPVTKLAEAESAKLL
                     RMEKELKKRVIGQEEAVSAVARAIKRGRIGLKEKNRPIGSFLFLGPTGVGKTELSKAL
                     SEALFGTENALIRVDMSEYMEKHTV"
BASE COUNT          708 a          402 c          661 g          459 t
ORIGIN      
        1 atatatatca caggaaagag atcaaaacaa aaaggaaatg cgtatgatag tagaaataga
       61 ctttaacagt gaagaagcaa tttatataca gcttcgaaat cagatcgtaa tcgggatcgc
      121 cacctcacaa ttaaaggaag gggatacact gccgtctgtc cgtcagcttg cggaactggc
      181 cggcatcaat atgcatacgg tcaataaagc atatgctgtt ttaagggatg aaggttttgt
      241 taagatcgat cgcaggaagg gtgctgtgat cgcactggat atcaataagc tgaaagccat
      301 ggaggaaatc aaagaggagc tgctgatcct gcttgcaaag gcgagcctta agaatattac
      361 ggcggctgaa atccatgaga tgatcaacga gatctatgca gagtacggat gtacgatcga
      421 gtgatatgag taaaagaata tgataaggag actgactaat ggcattgaaa tatacggata
      481 aagcagaaaa agcgctggaa ctggcaggca ggatctcgcg ggagttaaaa aacggttata
      541 tcgggacaga acatctgctt ttaggactga tcaaggaaga aacgggagtg gcggcaaaag
      601 tgctgaccgg aaatggtata tcagcggaaa gcattgttga gctgatcagg cagcagatcg
      661 cttttacatc gccggcaaat atcagggagc gtgacggatg gacgccgaag gcagaagcaa
      721 tcattgaaaa gagcggggat gaagcgtttc ggtttagggc agaaaggatc gggacagagc
      781 acttgctgct tgcgcttctt aatgacaaca gcagcctggc tgtccggctg ctgaatacgc
      841 tcggggtcaa tcttcagaag cttcatgtgg acatactgct ggcaatgggc gaagacgtgg
      901 ccaaatataa agaagagctg cagaatgcaa agcttgggaa gaaaaagaac caggggagcg
      961 gtcttcttgt ggatcagtac agcagggatc tgactgcgct tgccacagaa ggaaaactgg
     1021 atcctgtcat cggacgggaa atggaaatct cacggatcat tcagacgttg agcaggcgga
     1081 caaagaacaa tccctgcctg ataggagaac cgggagtcgg taaaactgcg atcgtggaag
     1141 gacttgcaag aaggatcgta gaagggcttg tgccggagac tgtgcagaac aaacgcgtac
     1201 tgacgcttga tctgtcaggg atggttgcag gcagtaaata ccgcggtgag ttcgaggagc
     1261 ggatcaagag agtcgtgaaa gaggtgacgg aagccggaaa tattctgctg tttctggatg
     1321 aaatgcatac gatcatcggc gccggcggcg cagagggagc gatcgacgct tccaatatat
     1381 taaaaccgtc tcttgcaagg ggtgagatcc agctgatcgg agcgacgaca tatgaggaat
     1441 atcataaata tgtacaaaaa gatgctgcat tggagcgacg gttccagccg atcatggtag
     1501 aagagccaag tgaagaaatt accgttcaga ttctggatgg gatcaagaat aattatgaga
     1561 agcatcatgg tgttgaaatt acggaggaag ccgtagcggc agccgtacag atgtcggtac
     1621 gctatatcaa tgaccgcttt ctgccggata aggcaattga tctgatggat gaagccgctt
     1681 ccaaagtgcg cctgaaagga ttgaaggcgc cggcgggtct tgaagagctt gaacagcagc
     1741 tgcagaattt tgaagaaaga aaagaacagc ttctgaagaa agatctgata gaagaatgcg
     1801 gtgaactgcg caaagaagag gaagcagtcc ggaaagtcta tgaaaagaag aaaaaacgtt
     1861 ttgaaaacag catgaaagga aagaagcctg ttgtagatgc ggatgaaatc gctgatgttg
     1921 tatcggaatg gacaaaaatt ccggttacaa agctggcgga agcggagagc gcaaagcttt
     1981 tgcgtatgga aaaagagctt aaaaaacgcg tgatagggca ggaagaagcg gtcagtgcgg
     2041 tggcacgggc gatcaaacgg ggcaggatcg gtttgaaaga gaaaaatcgt ccgatcggtt
     2101 cgtttttgtt tctggggccg accggtgtcg gcaagacaga gctgtcaaaa gcactttccg
     2161 aagcactttt tggaacggag aatgctctga tccgtgtaga tatgtcggaa tatatggaga
     2221 aacataccgt
//
LOCUS       DAOSYE010000360         2220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39370_length_2220_cov_2.999538, whole genome shotgun sequence.
ACCESSION   DAOSYE010000360 DAOSYE010000000
VERSION     DAOSYE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39370_length_2220_cov_2.999538"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLU43_06860"
     CDS             <1..711
                     /locus_tag="PLU43_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOO28169.1"
                     /translation="KQSVQFYKTTQGAFDITIAPVSSQWDFTSEDPVLPDDDAIKAAL
                     PLISYQNIVLDEKTQSVSLKDPNSAIDLGAIAKGYIADKLKEYLAGEGVTSAIIDLGG
                     NILTVGEKLDGSDFCVGIKEPFSKTGEVAAAVYVKDKSVVTSGTYQRCFTLDGTLYHH
                     ILDPCTGYPVNTDLNSVTIISGHSVDGDALSTTCMLLGMDKGLTLIEGLPDTEAIFIT
                     TDNALVYSSGMDAYLASP"
     gene            complement(769..1572)
                     /locus_tag="PLU43_06865"
     CDS             complement(769..1572)
                     /locus_tag="PLU43_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOO28170.1"
                     /translation="MDIQGIMIAVVCVGGVGLLIGVFLSFFGNYFKVETDPREDAVID
                     ALPGNNCGACGYAGCSGMAAAIVKGEAPVSGCPVGGEKTAAKVAAIMGVDAGKTKRMV
                     AYVKCRGTCEKTTVDYEYHGVEDCKMLSFVPNGGAKTCDYGCLGFGSCKKVCDFDAID
                     ILDGVAVINKEKCKACGKCVDNCPKNLIEMIPYEASYVVGCSSKDKGPVVMKACSAGC
                     IGCGLCEKACPVGAITVTDFIAHIDQEKCTGCGACAAKCPKKIIEAVCL"
     gene            complement(1584..2159)
                     /locus_tag="PLU43_06870"
     CDS             complement(1584..2159)
                     /locus_tag="PLU43_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOO28171.1"
                     /translation="MGELLAIAIGSAFINNVVLSQFLGICPFLGVSKKVKTAAGMGTA
                     VIFVITLSSAVTGVVYQFILIPLGITYLQTIVFILVIAALVQFVEMFLKKKMKSLYQA
                     LGVYLPLITTNCAVLGVALTNVQKEYGIFQGIVNGFAVAVGFLIAIVIMAGLRESMEY
                     NDVPKPFKGMPIVLLTAGLMAIAFFGFSGLK"
BASE COUNT          635 a          607 c          386 g          592 t
ORIGIN      
        1 aaacagtcag tgcaatttta taagacgaca caaggcgcat ttgatattac aattgcccct
       61 gtttccagcc agtgggattt tacttccgaa gatcccgttc tgccggatga tgatgcaatc
      121 aaagcggcgc tgccgcttat ctcttatcag aacatcgttc tcgacgaaaa aacacagtcc
      181 gtgtctttaa aagatccgaa ctctgccatt gatctcggtg cgatcgcgaa gggatatatt
      241 gcagataaac tgaaagaata tcttgccgga gaaggagtca catccgcaat catcgatctc
      301 ggcggcaata tactgactgt cggcgaaaag ctcgacggct ccgatttctg tgttggaatc
      361 aaggaacctt tttccaaaac cggtgaagta gctgccgccg tctacgtaaa agataaatct
      421 gttgtgacat ccggcactta tcagcgctgc ttcacacttg acggaacact ttaccatcat
      481 atcctcgatc cgtgtacagg atatccggtc aacacagatt taaacagcgt cacgatcatt
      541 tccggccatt ccgttgacgg cgatgcactc agtacgacct gtatgctgct tggcatggat
      601 aaaggactga cactgatcga aggtcttcct gatacggaag ccatctttat taccacagat
      661 aacgcacttg tttattcaag cggcatggac gcttatctgg cttctcccta acaaaaaacg
      721 tatgcatcct tcgaaaggat ccatacgttt tttataagcc aacgtttttt ataaacagac
      781 agcctcaatg attttcttcg gacatttggc cgcacatgct ccgcagcccg tgcatttttc
      841 ctgatcgata tgcgcaatga aatccgtaac ggtaatcgca ccaaccggac aggccttttc
      901 acataaacca cagccaatac atcctgccga gcaggccttc ataacaaccg gccctttgtc
      961 tttagagctg caccctacaa cataggacgc ctcatacggt atcatctcga tcaaattctt
     1021 cggacagttg tcaacacatt tcccgcatgc tttacatttt tctttattaa tgactgcaac
     1081 tccgtccaaa atatcaattg catcaaaatc gcacaccttt ttacagcttc caaagccgag
     1141 acatccgtaa tcgcatgtct ttgcgccgcc atttggtaca aatgaaagca ttttacagtc
     1201 ttcaactcca tgatattcat aatctaccgt tgttttttca caggtgcccc tgcactttac
     1261 atacgcaacc atccgcttcg ttttaccggc atctactccc ataatggcag ctaccttggc
     1321 agcagttttt tctccgccga ccgggcagcc ggaaacaggc gcttctcctt ttacgatcgc
     1381 cgcagccata ccggaacagc ccgcatatcc gcaggcacca caattattac ccggcagtgc
     1441 atcaattacc gcatcttctc ttgggtctgt ttccactttg aaataattac caaaaaagct
     1501 caaaaataca ccgatcagca aaccgacccc gccaacacag acgaccgcta tcataattcc
     1561 ctgtatatcc atattcctac ctcctatttc aagcctgaaa atccaaagaa tgcaattgcc
     1621 atcaagccgg ccgtaagcaa aacgatcggc attcctttaa acggcttcgg tacgtcattg
     1681 tattccatac tttctctcaa acctgccatg atcacgatcg caatcagaaa accgaccgca
     1741 acggcaaatc catttacaat accttgaaaa atcccatatt ccttctgtac atttgtcagc
     1801 gccacgccaa gaaccgcaca gtttgtcgta atcaaaggaa gataaacacc aagtgcctga
     1861 tacagacttt tcatcttctt tttcaaaaac atttccacaa actgcactaa cgcagcaata
     1921 accagaataa atacgatcgt ctgcaaatat gtaatgccaa gaggtatgag tatgaactga
     1981 tagaccacac cggttactgc agaagaaagc gtaataacga aaataactgc agttcccatt
     2041 ccggctgctg ttttcacttt tttcgataca ccaagaaacg gacaaattcc caaaaactga
     2101 cttaacacaa cattattaat aaatgcggat ccaatcgcaa tcgctaataa ttcacccatc
     2161 gcttattcct ccccattctt attttttgct atatttttcg acttcactgc gcctttttcg
//
LOCUS       DAOSYE010000361         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39477_length_2216_cov_3.471078, whole genome shotgun sequence.
ACCESSION   DAOSYE010000361 DAOSYE010000000
VERSION     DAOSYE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39477_length_2216_cov_3.471078"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLU43_06875"
     CDS             <1..391
                     /locus_tag="PLU43_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOO28172.1"
                     /translation="DAFTKIYIKVGLNELGWGTDELFLEKYAELIARLREVEPHAIIY
                     VHGLIPVTAEKSSTDSTHTNEKITARNELLAQFAVEQKAYYITINSIVSDDDGCLKDE
                     MTTDGIHLKSQYMDLWREYLRTHAVVH"
     gene            430..903
                     /locus_tag="PLU43_06880"
     CDS             430..903
                     /locus_tag="PLU43_06880"
                     /inference="COORDINATES: protein motif:HMM:NF025628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HOO28173.1"
                     /translation="MKKQKKMMEIIGLLFVVMLLAVGCGKKAASIDVIEVANELLEGI
                     PYQDALTAIDFDTADMIFNFSDVTIVNGAIFESSGATAEEIVVLECASAEDAKAAKTA
                     LETRVSEQEESFEDYVPEELVKLNEAVIVTSGKYAILSVSNEPDEAEKIIDGFIR"
     gene            complement(917..1528)
                     /locus_tag="PLU43_06885"
     CDS             complement(917..1528)
                     /locus_tag="PLU43_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOO28174.1"
                     /translation="MNPKQEAYKKQAQTIIKNLEKRNMEGHYFADSKECTNYILDMIP
                     MGSSIANGGSMTITDIGLLDALKGENYQYIDRMKESFSKVCSANYYLMSTNAITLDGE
                     LVNIDGAGNRVSCLIYGPDNVIVVAGMNKVTTTVTDAQNRVRNTASPANTTRLNKNTP
                     CAVTGVCGDCLSPDCICNQIVITRRSGIKNRIKVFLIGEELGY"
BASE COUNT          733 a          354 c          513 g          616 t
ORIGIN      
        1 ggatgcattt actaagatct atatcaaggt cggtttgaat gagcttggct ggggaacaga
       61 tgagctgttt cttgaaaaat atgctgagct gattgccaga cttagagagg tcgagcctca
      121 tgcgatcatt tatgtacatg ggttgatccc ggtcactgca gaaaaatcca gtacggacag
      181 tacccataca aacgaaaaga tcacggcgag gaacgagctg cttgcgcagt ttgccgtgga
      241 gcagaaggcg tattatatta ctattaattc gattgtttcc gatgatgacg gctgcctgaa
      301 ggatgagatg acaacagacg gaattcatct gaaatcgcag tatatggatc tgtggagaga
      361 atatttaaga acgcatgctg ttgtacatta agaccgggtg tgcaccattg agaggagaaa
      421 gatataatta tgaaaaaaca gaaaaaaatg atggaaataa tcggactgct gtttgttgtg
      481 atgcttttag ccgtcggatg cggaaagaag gcagcatcga tcgatgtgat cgaagtggcc
      541 aatgagctgc tggaaggaat tccttatcag gatgcgctga ctgcgatcga ttttgacacg
      601 gcggatatga tatttaactt ttctgatgta accattgtaa acggcgctat ttttgaaagc
      661 agtggtgcga ctgcggaaga aatcgtcgtg ttggagtgtg cgagcgccga agatgcgaaa
      721 gcggcgaaga ccgcacttga gacaagggta tccgaacagg aagaatcttt tgaggattat
      781 gtacctgaag agcttgtgaa actgaatgag gctgtgatcg ttacaagcgg aaagtacgcg
      841 attctttccg tttcaaatga gccggatgag gcagaaaaga tcattgacgg atttatcagg
      901 taaagcggag ctgggatcaa tatcccagct cttctcctat caaaaatacc ttgatgcggt
      961 ttttgatgcc gcttcgcctt gttataacga tctgattgca gatacagtca ggtgagaggc
     1021 aatctccgca tacgccggta acagcacacg gcgtattttt gtttaaacgc gtcgtattgg
     1081 ccggagacgc ggtgttacgt actctgtttt gtgcatcggt gacggttgtc gttactttgt
     1141 tcatacctgc gactacgata acattgtccg gtccgtaaat caggcaggac acacggtttc
     1201 cggcaccatc aatattaacg agttctccgt caagcgtaat ggcatttgtg ctcatcaggt
     1261 aatagtttgc ggagcagact ttagaaaatg attctttcat acgatcaatg tattgatagt
     1321 tttcgccttt tagggcatct aacagtccga tatcggtaat tgtcatggaa ccgccgtttg
     1381 caatggaact tcccattggt atcatgtcta aaatataatt tgtgcattcc ttgctgtctg
     1441 caaagtagtg tccttccata tttctttttt caaggttctt gataatggtc tgcgcctgtt
     1501 ttttataggc ttcctgtttt ggattcataa aatacctcct gttaaaataa ttatgttatt
     1561 tttattctaa cgaatatgat ataaattatc aatgtgaaaa tgaaacatta aattgtataa
     1621 aaaggacaaa aaaaagaaat tatttagaca tcaataacga ataataaaga gataagtttc
     1681 atataattag aaaacataaa aataaacata aaaaagtatt gacaattata agcgtttgaa
     1741 ttataatcaa atcataaaaa gaagaggcgg agaaaagaaa aaagtaagat ttataaataa
     1801 gaaaaggagg tacggaccac caaaatatta attttataaa tatcctttat ttgacaaatg
     1861 acatttagta agtacatcag ttataattag ggattcaggt aaccgggaaa agaaataagc
     1921 tcagtataat agattttgaa gtaatataaa tacgaactgt tggcgagtat ataaataaga
     1981 tcaggaactg agaagtaaac gtacaggtga tataaaaaat gcgatgtaaa gcaaggtaca
     2041 tcatgataaa tgaaaatgta cagaaaataa ttatccgacg tacggaagaa attacaatta
     2101 aatatggatg gtagaacgga aaatctaccc gagagaagga atcgggtatg gtacctgaaa
     2161 aactataaga tgtacaagca gaattgagag gtatagtcca ttggaaagtg acagtg
//
LOCUS       DAOSYE010000362         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39675_length_2210_cov_3.100232, whole genome shotgun sequence.
ACCESSION   DAOSYE010000362 DAOSYE010000000
VERSION     DAOSYE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39675_length_2210_cov_3.100232"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLU43_06890"
     CDS             <1..200
                     /locus_tag="PLU43_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28175.1"
                     /translation="NTVAEYLYPELSCEQINNHLLEEMVKKGLNGTSTQKGFYNYTEA
                     SVMDEEKERDNKMLAVLKLSS"
     gene            361..1332
                     /locus_tag="PLU43_06895"
     CDS             361..1332
                     /locus_tag="PLU43_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28176.1"
                     /translation="MRVRNNRVWIGIVIANVLLICAGLHFFFSDRDYSALQIEHSEKI
                     GACYADLSNSFFDVLNNEISSVVEEHGDILITRDSMLSQEKQNEQIRQLIADGVKALI
                     IAPVDWKEITPALEEAKTSDVIVIIVDAQVYDTGLVDCTVTSDNYDVGIQMSNFLMSQ
                     KPSAKILLLEQEGSSSSSDRAAGFKEGIRKFGAYRITAEEQCSGTVESAMQTVNNCIM
                     QGEEFDCVFSVNDYIAVGAITACEKNGIVEEVSFLSTDGSPEGKKLIDSQKMMATAAQ
                     FPGEMGGKAVDALYKIINGVECEKNIKVPVKLITRYTINSYDLEKWQ"
     gene            1352..>2210
                     /locus_tag="PLU43_06900"
     CDS             1352..>2210
                     /locus_tag="PLU43_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO28177.1"
                     /translation="MGVQEKKKVISGELALFYIRRIMKNLNLLIILWISGIICLTTYK
                     IVGAGEAQAFIEQTEKIPIEPWKVPLIALAAYFILLTIMSLRCFHLEKEKNFILYGTV
                     EVLLCMVIMGALNFNYNSIILLAIADLFIYWKNTKLRITAFIFLFILYIITDAAVYQN
                     FEQMIPLSTYLTYYNAEAKTILISVKSILCSCNILLFMFYMILLIRVQRIENERILDL
                     NRQLDEANEQLKIYAINTEKMAETRERNRLAREIHDTLGHVLTGIVAGIDACKKLIDY
                     STEETKEQLD"
BASE COUNT          701 a          358 c          546 g          605 t
ORIGIN      
        1 ttaacacagt ggcagaatac ctttatccgg aactttcctg tgaacagatc aataatcatc
       61 tgctggaaga aatggtgaaa aagggactta acggaacgtc cacacagaag ggattttata
      121 attatacgga agcatcggtc atggatgagg aaaaagagag ggataataag atgcttgccg
      181 tgctgaagct gtcctcgtga tcgataccgt gtatgtttcc ctttatgaca tttgtaatat
      241 gacagatgca agttgtacag cagcttgttt ttcgctataa ttataaatat agattattga
      301 aaaaagaaaa gattttttta aaaaggtgac aaatgtcatc agagtagata tggggaccat
      361 atgcgggtaa gaaataatcg agtgtggatc ggtattgtaa tagccaatgt gctgctgata
      421 tgtgcaggat tacatttttt tttctccgat cgggattatt cggctttaca aatagagcat
      481 agtgaaaaaa taggcgcctg ttatgctgat ctgagtaatt ccttttttga tgtattgaat
      541 aatgaaatca gttctgttgt tgaagaacac ggggatattc tgattacgag agattccatg
      601 ctgagtcagg aaaaacaaaa tgagcagatc cgtcagttga tcgcagacgg ggtaaaagcg
      661 ctgatcatag cgccggtgga ctggaaagaa atcacgccgg cactggaaga agcaaagaca
      721 tcggatgtaa ttgtcatcat tgttgacgcg caggtatatg ataccgggct ggtcgattgt
      781 acggttacat ccgataatta tgatgtcgga attcaaatgt caaatttttt aatgagtcag
      841 aagccttccg ccaaaatact tttgctggaa caggaaggtt ccagttcgtc atctgaccga
      901 gcggcaggtt ttaaagaggg aatccgaaag tttggcgcat atcggatcac agcggaagaa
      961 cagtgcagcg gaacggtcga atcggccatg cagaccgtaa ataactgtat tatgcagggg
     1021 gaggaattcg attgtgtgtt ttccgttaat gattacatag cagtcggcgc aatcacggca
     1081 tgtgaaaaaa atggaatcgt tgaggaggta agctttttga gtacggacgg cagtccggaa
     1141 gggaaaaaac tgatcgattc ccagaaaatg atggcaacgg ccgctcagtt tcccggtgaa
     1201 atgggaggaa aagcggttga tgcgctgtac aaaattataa atggcgtgga atgtgaaaaa
     1261 aatataaagg tgccggtaaa actgataacg cggtatacga taaacagtta tgatttggaa
     1321 aagtggcaat aagcttagcg gagtgagaaa attgggtgtt caggaaaaaa agaaagtaat
     1381 atcaggagaa cttgcacttt tttatattcg aagaatcatg aagaatctga atctgctgat
     1441 catattgtgg atctcgggga tcatctgtct tactacatat aaaattgttg gggctggaga
     1501 ggcacaggct tttatagaac aaacggagaa gatccctatt gaaccgtgga aggtgccgct
     1561 gatcgcattg gccgcctatt ttattctgct cacgatcatg tcccttcgct gctttcatct
     1621 ggaaaaagaa aaaaatttta ttctatacgg aacagtagaa gtattgctgt gtatggttat
     1681 tatgggagcg ctgaacttca attataacag catcatattg ctggcgatcg ccgatctgtt
     1741 tatttactgg aaaaatacaa agctgagaat tacagccttt atctttttgt ttatcttata
     1801 tattattacg gatgccgccg tttatcagaa ttttgagcag atgataccgc ttagtacgta
     1861 tctgacgtat tataatgcgg aagcaaaaac aatactgatc agtgtaaaaa gcatcctgtg
     1921 ttcctgtaat attttgttgt ttatgtttta tatgattctg cttatccgtg tgcagcggat
     1981 agaaaatgag agaattctgg atttaaacag acaattggac gaagcgaacg aacagctgaa
     2041 aatttatgcg atcaatacgg aaaaaatggc ggaaaccaga gagagaaacc gtctggccag
     2101 agagatccat gatacgctcg gacatgttct gaccggtatt gtggcaggaa tcgatgcatg
     2161 taaaaaactg atcgattatt caacggagga aacgaaggaa cagctggacg
//
LOCUS       DAOSYE010000363         2209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39693_length_2209_cov_3.679201, whole genome shotgun sequence.
ACCESSION   DAOSYE010000363 DAOSYE010000000
VERSION     DAOSYE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39693_length_2209_cov_3.679201"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLU43_06905"
     CDS             complement(<1..300)
                     /locus_tag="PLU43_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOO28178.1"
                     /translation="MLAGIDAVIFDLDGTLVDSMWIWKAIDREYLKTVNRTLPDDLQL
                     CIEGKSFYETAAYFKERFALRESVEEIMDTWNEMAFEKYAKEVTLKNNVLKFLDLL"
     gene            complement(322..1080)
                     /locus_tag="PLU43_06910"
     CDS             complement(322..1080)
                     /locus_tag="PLU43_06910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO28179.1"
                     /translation="MDKKIRLDKLLSEHADRTRSQVRASIKNGMVRVNGSVIKEAGYK
                     ISAADRVSFDGQPVIYEEFSCYMLNKPIGCVSATKDNLSRTVLDLLKDVSTKKMFPVG
                     RLDKDTEGLLLITDDGMLAHQLLSPKKHVEKTYYAILDDVLTEQNMEALKTGIEIGDE
                     KPCLPAAVKKCGEETDRPEFQGISTQAYEITIVEGRFHQVKRMFAKCGRQVLYLKRIS
                     MGTLVLDRNLKPGEFRRLTETEERLLRTDHGKTE"
     gene            complement(1083..>2209)
                     /locus_tag="PLU43_06915"
     CDS             complement(1083..>2209)
                     /locus_tag="PLU43_06915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOO28180.1"
                     /translation="PSAMLPADRLPIKEGDRVLDLCAAPGGKSTALLAKLHGTGFLLS
                     NDISYSRAQALLKNMEMHGASNFCVTAEEPQKLAAYYPGFFDKILVDAPCSGEGMFRK
                     DAALISSWETKGPDYYAPIQLAILSAAVRMLKPGGLLLYSTCTFSEKENEGVIRELLN
                     SNPELTTAAISPYDGFSQGRMGLTDCVRIFPHRMAGEGHFLALLKKENTPVSRAAESP
                     SVLQKNGRSFGQAGADLEAFLSFLDFSLPKDRLLFLNGMLYLLPENYTDSYHEALRYV
                     RSGILIGEIDKRGTFKPSQAFAMHLSYDRFRNTMDLALADLRVIKYLKGETIFYSSDE
                     VRLEKGYVLICVDGYPLGFGKVSVAGTIKNKYKPGWRMMG"
BASE COUNT          560 a          557 c          442 g          650 t
ORIGIN      
        1 taaaagatca agaaatttca gcacattatt ttttagtgta acttcttttg catatttttc
       61 gaatgccatt tcattccagg tgtccataat ttcttccacc gattccctaa gggcaaagcg
      121 ttccttaaaa tatgctgccg tttcataaaa actctttccc tcgatacaaa gctgcaggtc
      181 atccggcagc gttctgttta ctgtttttaa atattccctg tcaattgctt tccagatcca
      241 catggaatct acaagcgttc cgtccagatc gaaaataaca gcatcaatac cagctaacat
      301 tttttattct ccgttatcgt tttattctgt tttgccatgg tctgtccgaa gcagccgttc
      361 ttccgtttcg gtaagtctgc ggaattcccc tggctttaaa tttctgtcca gtacaagtgt
      421 acccatggag atccttttta aataaagcac ctgtctgccg cattttgcaa acattctttt
      481 aacctgatga aatctgccct ccacaattgt aatttcatag gcctgcgtac tgattccctg
      541 aaattccggt ctgtcggttt cttcgccgca ttttttaaca gccgcaggaa gacacggttt
      601 ttcatctccg atttctattc cagtctttaa ggcttccata ttctgctctg tcagcacatc
      661 gtcaagtatg gcataatacg ttttttcaac atgctttttc ggtgaaagaa gctgatgtgc
      721 aagcatgccg tcgtcagtga tcagcaacaa accctcggta tccttatcaa gccttccgac
      781 aggaaacatt tttttcgtac tgacatcctt tagcagatca aggactgttc tgctcagatt
      841 gtcctttgtg gcactgacac agcctatagg cttgttcagc atatagcagg aaaattcttc
      901 atagataaca ggctgtccgt caaaagagac cctgtcggct gctgaaattt tataaccggc
      961 ctcttttatg actgacccgt tcacccggac catgccgttt ttaatagaag ccctgacctg
     1021 acttcttgtt ctgtcagcat gctcagaaag aagtttgtca agcctgattt ttttatccat
     1081 ggtcacccca tcatccgcca gcccggttta tatttattct tgatcgttcc ggctacagat
     1141 accttgccaa aaccaagcgg gtatccgtcc acacagatca gtacatatcc tttttcaaga
     1201 cgaacctcgt cagaagaata aaatatcgtc tctcctttta aatacttaat gacacgcagg
     1261 tctgcaagtg caagatccat tgtgttccgg aagcggtcat aagaaaggtg catggcaaac
     1321 gcctgactcg gtttaaaggt cccgcgcttg tcgatctcac cgatcagaat tccgcttctg
     1381 acgtatcgta aagcttcatg ataactatct gtataatttt ccggaagcag gtaaagcatt
     1441 ccgtttaaaa acaaaagcct gtcttttgga agcgaaaaat caagaaaaga taaaaaagcc
     1501 tccagatcag cgcctgcctg cccaaagctc cggccgttct tttgcaaaac ggaaggagat
     1561 tccgccgccc tgctgacagg ggtgttttct ttttttagta atgccagaaa atgcccttcc
     1621 ccggccatac ggtggggaaa aatccttaca caatccgtca gacccatacg gccctgtgaa
     1681 aatccatcgt atggactgat tgccgccgta gtcagttcgg gattcgagtt gagcagctct
     1741 ctgattacac cttcattctc tttttcggaa aaggtacagg tcgaatataa aagcagtccg
     1801 ccaggcttca gcatcctgac cgctgccgaa agtattgcaa gctgtatcgg cgcataatag
     1861 tcagggcctt ttgtttccca gctgctgatc agagctgcat ctttccggaa catgccttct
     1921 cccgaacacg gggcgtctac caggatcttg tcaaaaaaac caggataata tgcggcaagt
     1981 ttctgtggtt cttcagccgt aacacaaaaa ttggaggcac cgtgcatctc catgtttttc
     2041 aggagcgcct gggctctgga ataactgatg tcattggata aaagaaaacc tgtcccatga
     2101 agctttgcaa gaagtgctgt tgatttgccg cctggcgccg cacacagatc cagcacacga
     2161 tcgccttctt tgatcggaag cctgtctgca ggcagcattg cgcttggtt
//
LOCUS       DAOSYE010000364         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_39798_length_2206_cov_3.518364, whole genome shotgun sequence.
ACCESSION   DAOSYE010000364 DAOSYE010000000
VERSION     DAOSYE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39798_length_2206_cov_3.518364"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLU43_06920"
     CDS             complement(<1..210)
                     /locus_tag="PLU43_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOO28181.1"
                     /translation="MPSQFSRTEMLLGEKAMEKLRTSAVAIFGIGGVGGYAAEALARS
                     GVGRLALYDNDVVCLTNLNRQIIALH"
     gene            complement(297..875)
                     /locus_tag="PLU43_06925"
     CDS             complement(297..875)
                     /locus_tag="PLU43_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO28182.1"
                     /translation="MDTKQFLIFLGKIEKLKSNTRHSYTADGTHEVVASHTWRLAVMA
                     MLLEREFPALDMDKVIRMCLIHDWGEAVTGDIPSFDKTKEHEDIETQAVEGLIRTLPK
                     ELYQDMAVLFEEMDALETPEARFYKALDKLEAVISHNESDISTWIPIEYDLQMTAATE
                     EVKGFPFLEQLREQMKQDTKEKIAKAGTQHAD"
     gene            1002..1075
                     /locus_tag="PLU43_06930"
     tRNA            1002..1075
                     /locus_tag="PLU43_06930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1036..1038,aa:Pro,seq:cgg)
     gene            complement(1125..2066)
                     /locus_tag="PLU43_06935"
     CDS             complement(1125..2066)
                     /locus_tag="PLU43_06935"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOO28183.1"
                     /translation="MKNVVVAGGGVLGAQIAFQAAYCGFDVTIWLRSEGSISRTQPKI
                     DRLRKVYKDTIRTMATAEGKNAENWARGLAEYDTFDADDCLAKAEKAYSGIKIELDLA
                     KAAEDADLIIESMAENADEKIIFYKKLAPLMPEKTILVSNSSTLLPSKFAKSTGRPDR
                     YLSLHFANSIWKNNMAEVMAQDKTKQEIFDQVIQFAKEIRMVALPVKKEKAGYLLNSM
                     LVPLLFSGMDLYVNGISDPESIDKAWTLGTGAPKGPFRILDTVGLITAYNIVQQYVKL
                     PSFLAPYNFKGMSKMLKEYIDAGKLGMSAGEGFYKYH"
BASE COUNT          565 a          546 c          387 g          708 t
ORIGIN      
        1 gtgcagtgca atgatctgcc ggttcaaatt tgtcagacag acaacgtcat tatcataaag
       61 cgccagcctg ccaactccgc tcctcgccag tgcctctgcc gcgtaaccgc cgactccgcc
      121 gataccgaat atcgccacgg cagatgtccg cagcttctcc attgcttttt ctcctaataa
      181 catttctgtt cttgaaaatt gagacggcat tctttttccc ttcatttgtc aatatgttta
      241 agcgcagcat tctaaattaa tatagcatat ccgcaaatga atgaaaagca ggaatgtcag
      301 tctgcatgct gcgtgccggc tttcgcaatc ttttcctttg tgtcctgctt catctgctcc
      361 cgcagctgtt ccaaaaacgg aaatcctttt acttcctctg tagctgccgt catctgaagg
      421 tcatactcga tcggtatcca tgtactgatg tcgctttcat tatgagaaat aacggcctcc
      481 aacttgtcca gtgctttata aaatctcgcc tccggtgtct ccagggcatc catttcttca
      541 aaaaggactg ccatatcctg ataaagttcc tttggcagcg tacggatcag gccctcaaca
      601 gcttgtgttt caatatcctc atgctctttt gtcttatcaa aagaaggaat atcgcccgtc
      661 actgcctcac cccagtcatg aatcaggcac atcctgatga ccttatccat atcaagcgcc
      721 ggaaactctc tttctaacag cattgccatt acggctaagc gccatgtatg cgatgctacg
      781 acttcatgtg ttccatcggc agtatagctg tgtcttgtgt tggattttaa cttttctatt
      841 tttcctaaaa aaatcaaaaa ctgttttgtg tccatcttgc tcctccctga cttataaatg
      901 atgattacat ttatattttt taaaaatctt gttgattttt ttcattttta tggtatacta
      961 ggactgttcg cgaatcttct ttttcgcaga cgataggata tcggcgtgtg gctcagcttg
     1021 gtagagcgct acgttcggga cgtagaggtc gcaagttcaa atcttgtcac gccgacttaa
     1081 aaaggaagtg atccgtcact tccttttttg tggatatgat aagatcaatg atatttataa
     1141 aaaccttccc cggctgacat gccaagtttt cctgcatcaa tatattcttt aagcatcttg
     1201 gacatgccct taaaattata tggtgcaagg aatgacggca gcttaacata ctgctggacg
     1261 atgttataag cagtgatcaa tcctaccgta tcaagaatac gaaatggtcc cttaggcgct
     1321 ccggttccta atgtccaggc cttatcaata ctttccggat ctgaaatacc attcacatac
     1381 aaatccatac cggaaaaaag caatggcaca agcattgaat taagcaaata tcccgctttt
     1441 tcctttttga ccggaagggc aaccattctg atctccttgg caaattgaat aacctgatca
     1501 aatatttcct gctttgtctt gtcctgggcc ataacctcag ccatattatt cttccatatt
     1561 gagtttgcaa aatgcaatga taaataccga tcaggcctcc ctgtgctctt ggcaaattta
     1621 gacggaagca atgttgagga attagataca agaatcgtct tttccggcat cagcggtgca
     1681 agctttttat aaaagattat tttctcatct gcattttctg ccattgactc aataatcaag
     1741 tccgcatcct ctgctgcctt agctaaatcc agttctattt ttataccaga atatgctttt
     1801 tcagctttgg ccagacaatc gtccgcatca aatgtatcat actcagcaag ccctctggcc
     1861 cagttttctg cgttctttcc ctctgctgtt gccatggtcc tgatcgtatc tttataaact
     1921 tttcttaacc ggtctatctt tggctgtgtc cgcgaaatac ttccttcact tctaagccat
     1981 atggttacgt caaatccaca atatgctgcc tgaaaagcga tttgagcgcc aagtaccccg
     2041 ccgcctgcta ctacaacatt tttcatatct tactatcctc ccatacatta ttaaattcag
     2101 aaatattata acattggtgt gcagttttgc caatcaaatc aacgtcttta ataaaaaaag
     2161 aaaagccggc cttttcctgc ctgcttttct cattttctac tgtctt
//
LOCUS       DAOSYE010000365         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40076_length_2197_cov_3.079832, whole genome shotgun sequence.
ACCESSION   DAOSYE010000365 DAOSYE010000000
VERSION     DAOSYE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40076_length_2197_cov_3.079832"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLU43_06940"
     CDS             complement(<1..310)
                     /locus_tag="PLU43_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO28184.1"
                     /translation="MVEVILVSHGTYARALLESSELIMGEQECVYPFGFSLGENVEDL
                     RSRIEEKIVEIKKAHPEHEIMVLTDMKSGSPFNAAAFLMQSHSFIHIAGINLPIFLEI
                     L"
     gene            complement(324..797)
                     /locus_tag="PLU43_06945"
     CDS             complement(324..797)
                     /locus_tag="PLU43_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO28185.1"
                     /translation="MEEILRKENIKLDLAAESKMDVLKKLSELLFENGEIENAEAFFE
                     DVMERESLGFTGAGNQIAIPHGISDQVNKVVLAAAALTDPVIWDTKQQDIPEESKAVR
                     LVILFAVPKDPPADKEAAYIRVLKRVCVKLADAKSLQKLLAAVSASQIMDIINGE"
     gene            complement(807..>2197)
                     /locus_tag="PLU43_06950"
     CDS             complement(807..>2197)
                     /locus_tag="PLU43_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOO28186.1"
                     /translation="YSKKLNVSTKTLQSDLKKIRSFLKKYEIRINAVSGKGILLLQKN
                     RVREQLLNAMRPDDRKENEESSGERRNSILKNMLLHTNELTSIHKLSEQYFVGKTSIV
                     NDMKTIEKWLEKYNLELSKTKEGTCVKGSEINIRKAIAGLAIRENARSGLQELFEKED
                     IDFIEELLYDVEKKNLDISDVYYANLLTHILICIKRVRENIHIEENETSYMVNAETVE
                     QYQKASEIGKRIDEHYGIQIKEGEVYYIYQYLISSGVEKKTDTEEREWNRDDKSLRFA
                     KELTGLLSERFGIRFEQDTDMMQGLLLHIRPMINRLEYNIQIQNPLKEDITRQYPEMV
                     KSCGEILAKLAVKYDLKQISLDEAVNIAIYYQTMLEKKVMKKQVIVVCHSGYGTSQLL
                     AAKLQNAFAFLKIVDVVSSRKIKDMDLSGIDFIITTVPIERKDVPHIVVSSLLSGQDI
                     KAIRNCFAGQRE"
BASE COUNT          557 a          549 c          343 g          748 t
ORIGIN      
        1 caagaatctc cagaaaaatc ggcagattga taccggcgat gtggataaat gaatgactct
       61 gcattaaaaa tgctgccgca ttaaacggac ttcccgattt catatctgtc agaaccataa
      121 tttcatgttc cggatgcgct tttttaattt caactatttt ctcttctatt ctgcttctta
      181 gatcctccac attttctcca agagaaaaac caaaaggata tacacactcc tgttccccca
      241 taatcaattc gctgctctca agcaaagccc ttgcataagt tccgtgagag actaagatca
      301 cctcgaccat tttgtactcc tttctattcc ccgttaataa tgtccattat ctgtgaagca
      361 gatactgccg ccagaagctt ctgcagactt tttgcatctg ccagctttac acacactctt
      421 ttcaataccc gtatataagc tgcctctttg tctgccggcg ggtctttggg tactgcaaaa
      481 agaatgacca gcctgactgc ttttgattct tccggaatat cctgctgctt cgtatcccat
      541 ataacgggat ctgtcagtgc agccgctgca agaactactt tgttgacctg atctgatatt
      601 ccatgcggga tcgcaatctg atttcccgcc cctgtaaacc ccagactttc ccgttccatc
      661 acatcttcaa aaaaggcttc tgcgttttct atttctccat tttcaaaaag cagttcagac
      721 agttttttca gaacatccat cttagattct gccgccagat ccaattttat attttctttt
      781 cgcaagatct cttccatttt gaccgcctac tctctctgcc cggcaaaaca atttctgatt
      841 gccttgatgt cctgtcctga caacagtgac gatacaacaa tatgcggaac atcttttctc
      901 tcgatcggca ccgttgttat aataaagtcg ataccggaca gatccatgtc ttttattttc
      961 ctgctggaaa ctacatctac aatttttaaa aaagcaaatg cattctgcag cttggctgct
     1021 aaaagctgag acgttccata gccgctgtga caaacaacga tcacctgctt tttcatcacc
     1081 tttttttcaa gcattgtctg ataataaatt gcaatattta cggcttcatc cagactgatc
     1141 tgtttcagat catactttac ggccagcttt gcaagaatct ccccgcagct ttttaccatt
     1201 tccggatact gtctggtaat atcttctttc agagggtttt gtatctgtat attatattcc
     1261 agacgattta tcatcggacg gatgtgaagc agcagaccct gcatcatatc cgtatcctgc
     1321 tcaaaacgaa taccaaatct ttcagacaaa agtcctgtca gttctttcgc aaaacggagc
     1381 gatttatcgt ccctgttcca ttcccgctct tctgtatccg tcttcttttc cacaccggag
     1441 gaaatcaaat actgatagat ataatagact tccccttctt ttatttgaat tccgtaatgt
     1501 tcatcgatcc tcttcccgat ttcactggcc ttctgatact gttcgaccgt ttcggcattc
     1561 accatatagc ttgtttcgtt ttcttctata tgtatatttt cacgtactct tttgatacag
     1621 atcaaaatat gagtcaaaag attggcatag tatacgtcac tgatatccaa attctttttt
     1681 tccacatcat agagaagctc ttcaataaaa tcaatatcct ctttctcaaa caattcctgc
     1741 agaccgcttc ttgcattttc ccgtattgca agtccggcga tcgctttcct gatatttatc
     1801 tcgctgccct tgacgcaggt tccttctttt gttttgctca attccagatt atatttttca
     1861 agccactttt caatggtctt catatcattt acgatacttg tttttccaac aaaatattgc
     1921 tctgacaatt tatgaataga agtaagttca ttggtgtgca gcagcatatt ctttaaaata
     1981 gaattgcgcc gttctccgga agactcctca ttttccttcc tgtcatccgg tctcattgca
     2041 ttcagcagct gctcccggac ccgattcttc tgcagcagca aaataccttt tccagagacc
     2101 gcattgatcc tgatctcata tttttttaaa aagcttctga tctttttcag atctgattgt
     2161 aatgtttttg ttgaaacatt cagcttttta gaataat
//
LOCUS       DAOSYE010000366         2196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40107_length_2196_cov_3.179823, whole genome shotgun sequence.
ACCESSION   DAOSYE010000366 DAOSYE010000000
VERSION     DAOSYE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40107_length_2196_cov_3.179823"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1287
                     /locus_tag="PLU43_06955"
     CDS             313..1287
                     /locus_tag="PLU43_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOO28187.1"
                     /translation="MSKFQQIPYDPDNLESFDREWLCHRGVSEWWYATGYFYDENNCL
                     FSFQYTLVRALVCSVRIYILMLAVTDIKTGRHYFVQQPALSEKKIDIDEKTLRFCDTA
                     TLIKEKTGIHIKATAADFTYDLFLDYGKGAFRHCDNGRLQMGIPDKRATTRYYSYTNM
                     PAFGTITLDGITHTVTGKAWFDKQGGTYPITNPACMWEWFSLRFFDEEEIMLFSFPQD
                     DYMDGTYIRKDRTSMRLNRYTITPKKFTMSGGYRFSCEWELSLPGQKDKHYLIRPLTD
                     GQLNLVYFELLAGIYKDSGDLAGYCVAELLPGVYNKKINVLHLLKKQN"
     gene            complement(1284..2033)
                     /locus_tag="PLU43_06960"
     CDS             complement(1284..2033)
                     /locus_tag="PLU43_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28188.1"
                     /translation="MMEYKKVWLGIIIWAVLVFLFFNAAFAAGVTLSLSEKSGCEGDS
                     IEISGSGTPDEWIMIKSLDADGNIIFIKPVKAETDGTYSVTVIIPEVEAGILQIIAGS
                     GSDVKSVDFTVEERPDEEEESSSEAAESSSETVHEPALLEETKEPGIMPKLFVTKLPD
                     DTTESTAQASAKQAVKEPESNEEPEKIADEPEDEPMKEPEASPILPDEDKEPAGDVGS
                     IMLLLLCGAVLAAGTAVWFLRRMKKRKAESL"
BASE COUNT          555 a          577 c          442 g          622 t
ORIGIN      
        1 cgcgccgcat gtgaccactc cgttcacgat acttacactt ccctcagcca gatcgactgt
       61 gatcgttgta tccgctgcca ttaccggcag taccggctgc tgcaacagcc tgataaacac
      121 aaatgacact gctgccatca aaataatttt tctgatcctt tttttctttc tcatgcaaat
      181 ccctcttctc ttgaaatttt attttgacag cttactaccc atttggatgt ttttcattta
      241 taatatagac agcaataact gttacaaatg taacacgttg actctgtctg ttctgtatat
      301 aaggagaaaa ctatgtcaaa attccagcag attccctatg atccggacaa tttagaaagc
      361 tttgaccgtg aatggctgtg ccaccggggt gtcagtgagt ggtggtacgc taccggttat
      421 ttttatgatg aaaacaactg cctgttttcg tttcagtata cacttgtgcg cgcacttgtc
      481 tgttctgtac ggatctatat tctgatgctc gcagtgacag atatcaaaac cgggcggcat
      541 tactttgtcc agcagccggc cctgtcggaa aagaagatcg atattgatga aaaaacactg
      601 cggttctgcg acactgctac tctgataaaa gaaaaaaccg gtatccacat caaggcaacg
      661 gcagcggatt ttacctacga cctgtttctg gattacggga aaggcgcctt ccggcactgt
      721 gacaacggca gactgcagat gggcatccct gataagcggg caaccacacg atactattcc
      781 tatacgaata tgcctgcctt tggaacgatc acacttgatg gaatcacaca tacggtaaca
      841 ggaaaagcgt ggttcgataa acagggcggc acctatccca tcacaaatcc ggcatgtatg
      901 tgggaatggt tttccctgcg tttttttgat gaggaggaga tcatgctgtt ctcctttccg
      961 caggatgatt atatggacgg cacctatatc cgcaaagacc ggacaagcat gcggctgaac
     1021 cgctatacca ttacaccaaa aaaatttaca atgtcaggag gctatcggtt ttcctgtgaa
     1081 tgggagctct ctcttccggg gcaaaaagac aaacactatc tgatcaggcc gctgacagac
     1141 ggacagctga acctcgtcta ctttgaactg ctggccggca tttacaaaga ttccggcgac
     1201 ctggccgggt attgtgtcgc agaacttttg cctggtgtat ataacaaaaa aatcaacgtc
     1261 ctgcacctgc ttaagaagca gaattaaagg ctttccgcct ttcttttctt catccgccgc
     1321 aggaaccaga cagcagtgcc cgccgcaagc accgcgccgc acaacagcag cagcatgatc
     1381 gagcccacat cgccggccgg ctctttatct tcatccggta aaatcggaga agcttccggt
     1441 tccttcatcg gctcgtcttc cggttcatcc gctatttttt ccggttcttc atttgattcc
     1501 ggctctttca ctgcctgttt ggcggatgcc tgcgccgtac tttcagtcgt atcatccggc
     1561 agcttagtca caaacagctt tggcatgatg cccggctctt ttgtctcttc caaaagtgcc
     1621 ggctcatgta cagtttctga tgaggattcc gcagcttccg acgaggattc ctcttcttca
     1681 tccggtcttt cttctaccgt gaaatcaacg gatttcacat cgcttccgct ccctgctatg
     1741 atctgcagga tcccagcttc cacttccggt ataatcactg ttacgctgta agtcccgtca
     1801 gtttccgcct taaccggttt tataaaaata atattcccat ccgcatccag actcttgatc
     1861 atgatccatt cgtccggcgt accggaaccg gaaatttcga tactgtcccc ttcacatccg
     1921 cttttttcgc ttaacgataa cgtaacgcct gctgcaaagg cggcgttaaa aaacaaaaaa
     1981 accaatactg cccatatgat tattccaagc catacctttt tatattccat catttcctcc
     2041 tattacaaaa acagccactg tatattttac agtgactgct ttttcccgtg tactacccat
     2101 tcgggtattt ttatttattt ttgtttttaa aattccataa gtgaaagagc aattgcaata
     2161 aaaactgcaa ttccagtctc cagacaagcc tcatca
//
LOCUS       DAOSYE010000367         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40126_length_2195_cov_4.069159, whole genome shotgun sequence.
ACCESSION   DAOSYE010000367 DAOSYE010000000
VERSION     DAOSYE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40126_length_2195_cov_4.069159"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLU43_06965"
     CDS             <1..978
                     /locus_tag="PLU43_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO28189.1"
                     /translation="YATEHELSLYDALLQADQITALGRSAVKLKPFTEMIQVFRSKLP
                     YLTLEDLMNDIIKTTGYIESLEASDKEEAEDRVNNIEELLNKVIAFEQNADEPPSLSE
                     FLEEVALVADIDNVDRDDNKVLLMTLHSAKGLEFPQVYLAGMEDGLFPSYMNITSDDP
                     MELEEERRLCYVGITRAMEELTLTCARQRMVRGETQYNAVSRFVKEIPPLLLDRTPFA
                     KKVREEDIPSSSFTKYTKEIAKTKPYAMSAQERNAHVKPYIAGAVKNIGTIQTELDYE
                     VGDTVRHIKFGEGTVKNIISGGRDFEVTVDFQKTGIKKMFASFAKLKKI"
     gene            1175..1522
                     /locus_tag="PLU43_06970"
     CDS             1175..1522
                     /locus_tag="PLU43_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HOO28190.1"
                     /translation="MDSRLDDLVKTVKAQEALGVAKASDFINAVKANELLNLKKKEEE
                     KKKSNKAAWIIGIILGVAAIAAIAYGLYCYFTPDYLEDFEDDLDDDLNDEFDDEFFED
                     EDEVQKDESKEEK"
     gene            1631..>2195
                     /locus_tag="PLU43_06975"
     CDS             1631..>2195
                     /locus_tag="PLU43_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HOO28191.1"
                     /translation="MKQLMTYWGGQLNQKHVLEEYPRPLMQRNSYVNLNGLWDYAFTP
                     DKSKDLSAIGWEGRILVPFSPEAYLSGVNRQLKPGEYLWYRRDLPVPSDFEKGKRLLV
                     HFGAVDQQCEIYVNQKSVMTHSGGYLPFEADVTEVLMPAGENELIVCVRDDSDTSYYS
                     RGKQMLKSGGMFYTAQSGIWQTVWMECV"
BASE COUNT          678 a          378 c          592 g          547 t
ORIGIN      
        1 tatgccactg agcatgaact cagcctgtat gacgccctgc tgcaggccga tcagatcacg
       61 gcgcttggaa ggagtgcggt aaagctgaag ccatttacgg aaatgatcca ggtatttcgc
      121 agcaagctgc cttacctgac gctggaagat cttatgaacg atattatcaa gacgaccggg
      181 tatatagaga gtctggaggc ttctgacaaa gaggaagcag aggacagggt caataatatt
      241 gaagaactgc tcaataaggt cattgccttt gaacagaatg cggatgaacc gccgtcactt
      301 tccgagtttt tagaggaggt cgctcttgtt gccgatatcg acaatgtgga cagagatgac
      361 aataaagtgc ttttaatgac acttcacagc gcaaagggtc ttgagtttcc gcaggtctac
      421 ctggcaggga tggaggacgg tctgtttccg agctatatga atattacatc ggatgatccg
      481 atggagcttg aagaggagcg ccggctctgt tacgtgggaa ttacgcgtgc gatggaggaa
      541 ctgacgctga catgcgccag acagcggatg gtgcgcggag aaacacaata taatgcagta
      601 tcccgatttg taaaggagat cccgcctctt cttttagaca gaacgccttt tgcaaaaaaa
      661 gtgcgggaag aagatattcc gagttcttct tttacaaagt atacaaagga gatcgcaaaa
      721 acaaagccat acgcaatgtc cgctcaggaa agaaatgccc atgtaaagcc ttatattgca
      781 ggtgctgtga aaaatatagg gacgatccag acggaacttg attatgaggt gggtgatacg
      841 gtccggcata taaaattcgg agagggaact gtaaaaaata ttatcagcgg aggcagggat
      901 tttgaggtta cggttgactt ccaaaaaacc ggaatcaaga aaatgtttgc ttcttttgca
      961 aaactaaaaa aaatataaaa tttttcacaa agcaatagta aattcagaca gtttatggta
     1021 aactgattat agttttaata aaatttaagt aaggtttgta aaataattca tgttatgaca
     1081 atcagatgcc gatttagttt tgattttgat acggtatagt cataatttcc gataaatgcg
     1141 gattatagaa aatgaaagga tgggcggaga aatgatggat agcagattgg acgatttagt
     1201 aaaaacagta aaggcacagg aggcgctcgg ggtggcaaag gcaagtgatt ttatcaatgc
     1261 tgtgaaggca aacgagcttt taaatttgaa gaaaaaagaa gaggaaaaga agaaatccaa
     1321 caaagcggcg tggatcattg gaatcatcct cggagttgct gcaatcgcag cgatcgccta
     1381 cggattatac tgttatttta caccggatta tctggaggat tttgaggatg accttgacga
     1441 cgatctgaac gacgaatttg acgacgagtt tttcgaagac gaggatgaag tccaaaagga
     1501 tgagagtaag gaagaaaaat aagcgtagga tgttaggaag ctctgtgaat acgcagagct
     1561 tcttttttag ttcataggaa agagctccgc taaggatgcg cttctatata agaatggatg
     1621 gatatcggaa atgaaacagc ttatgacata ctggggcgga caattaaatc agaagcatgt
     1681 tctggaagaa tatccgcgtc cgctgatgca gcgcaacagt tatgtaaact taaacggatt
     1741 gtgggattat gcgtttacgc cggataaaag taaagacctg tcggcaatcg gctgggaggg
     1801 aaggatcctt gttccgtttt cgccggaggc ttatttatcg ggtgttaaca gacagttgaa
     1861 gccgggtgaa tatttatggt accgcagaga tctgccggtg ccttctgatt ttgaaaaagg
     1921 aaagcggctg cttgtgcatt ttggggccgt tgatcagcag tgtgaaattt atgtaaacca
     1981 aaaatcagtt atgacgcaca gcggcgggta tctgccgttt gaagcggacg tgacagaggt
     2041 gttaatgcct gctggggaaa acgaactgat cgtctgtgtg cgtgacgaca gcgatacttc
     2101 ttattacagc aggggaaagc agatgcttaa gagcggcggg atgttttaca cagcacagag
     2161 cggtatctgg cagacggtat ggatggaatg tgtcc
//
LOCUS       DAOSYE010000368         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40129_length_2195_cov_3.503738, whole genome shotgun sequence.
ACCESSION   DAOSYE010000368 DAOSYE010000000
VERSION     DAOSYE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40129_length_2195_cov_3.503738"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /gene="cutA"
                     /locus_tag="PLU43_06980"
     CDS             <1..377
                     /gene="cutA"
                     /locus_tag="PLU43_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOO28192.1"
                     /translation="HEALMKPILIFITCASTEEAQKISQELLQHHLVACASILPEIQS
                     HFHWQGKIQLENEILMILKTTEYHFQQISKKSLKKSSTKLSLKILGANISHSSLDLKF
                     IFTFSQNQTSCLFCYIIYNTSC"
     gene            complement(495..1979)
                     /locus_tag="PLU43_06985"
     CDS             complement(495..1979)
                     /locus_tag="PLU43_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOO28193.1"
                     /translation="MNNIPKVKIGIVAVSRDCFPEPLSVNRRKALVAAYTKKYDASDI
                     YECPICIVESEIHMVQALEDIKKAGCNALCVYLGNFGPEISETLLAKHFDGPSMFVAA
                     AEESQNDLVGGRGDAYCGMLNASYNLKLRNVKAYIPEYPVGTADQCADMINEFVPIAR
                     AVVGLSDLKIISFGPRPLNFLACNAPIKQLYNLGVEIEENSELDLFEAFKKHDGDPRI
                     ADVVKDMEAELGTGNKKPEVLPKLAQYEITLLDWIEAHKGYRKHVAIAGKCWPAFQTQ
                     FGFVPCYVNSRLTSRGIPVSCEVDIYGALSEYIGTCVSNDIVTLLDINNSVPDDMYEE
                     AIKGSYEYTHQDTFMGFHCGNTSSKKLTSCSMKYQMIMARNLPEEVTQGTLEGDIVPG
                     DITFYRLQSTADNVLRAYVAQGEVLPVATKSFGGVGVFAIPEMGRFYRHVLIEKNYPH
                     HGAVAFGHYGKALYEVFKYIGVAADEIGFNQPKGMMYKTENPFQ"
     gene            complement(1998..>2195)
                     /locus_tag="PLU43_06990"
     CDS             complement(1998..>2195)
                     /locus_tag="PLU43_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28194.1"
                     /translation="ESGEQRDRGTEGFEVLCERKEPDSKGKSRGTCTEGQTGKNPGSN
                     ATEEPKVSRCSAKKVSMYVIN"
BASE COUNT          602 a          549 c          391 g          653 t
ORIGIN      
        1 cgcatgaggc cctaatgaaa ccaattttaa ttttcatcac ctgcgcttcc actgaagaag
       61 cgcaaaaaat cagccaggaa ttgctgcagc accatttagt tgcctgcgct tcaattctgc
      121 cggaaattca gtcccacttc cactggcagg gaaaaattca gctggaaaac gaaattctga
      181 tgattctcaa aacgacagag tatcattttc agcaaatatc taaaaaatca ttgaaaaaat
      241 catctacaaa attgtctctg aaaatactcg gtgctaacat aagtcattcc tccttagact
      301 taaaatttat ttttacattt tcgcaaaacc aaacttcttg tttgttttgt tatataatat
      361 ataacacaag ctgttagcac tgtcaatagg tgagtgctaa ttttataaaa tgtttatatt
      421 tataaaaaaa attcccatcc tttctattct gaaaacaaaa ggatgggaac cactctttac
      481 ttatttaata tgttttattg aaatggattt tctgttttat acatcattcc ctttggctgg
      541 ttaaagccga tctcatcggc agcaacccca atatatttaa acacctcata aagggccttt
      601 ccataatgac cgaatgcaac agcgccatga tgcggataat ttttctcgat cagcacatga
      661 cgatagaaac gtcccatttc cggaatcgca aatacgccga ccccgccaaa agattttgta
      721 gcaaccggaa gcacttcacc ctgtgcaaca taagcacgaa gcacattatc cgctgtgctc
      781 tgaagtctgt agaacgtaat gtcacccgga acaatatctc cttcaagcgt accctgtgtt
      841 acttcttccg gaagatttct tgccatgatc atctgatatt tcatggaaca tgacgtaagc
      901 tttttcgaac tggtattgcc gcaatggaag cccatgaatg tatcctgatg cgtatattca
      961 tagctgccct tgatcgcttc ttcatacata tcatccggta cggaattgtt aatatcaagc
     1021 agcgtcacaa tatcattgct gacacaggtt ccgatatatt cactcaaagc gccgtaaata
     1081 tcaacttcac aggaaacagg aattccgcgg ctcgtcagac ggctgttaac atagcacgga
     1141 acgaatccga actgtgtctg gaatgccggc cagcattttc cagcgatcgc aacatgcttt
     1201 ctatagcctt tatgtgcttc gatccagtca agcagcgtga tctcatactg ggcaagcttc
     1261 ggcagaactt ccggcttttt gttgcctgtt ccaagttccg cttccatatc ctttacaaca
     1321 tctgcaatac ggggatctcc gtcatgcttt ttaaacgctt caaacagatc cagttctgaa
     1381 ttttcctcga tctctacgcc aagattatac agctgtttga tcggtgcatt acatgcaagg
     1441 aaattcaacg gtctcggacc gaagctgatg atctttaaat cggaaagtcc gacaacagct
     1501 cttgcgatcg gtacgaattc attgatcata tccgcacact ggtccgctgt tcctaccgga
     1561 tactccggaa tataggcctt tacgttacgc agctttaaat tatagctggc attcagcatc
     1621 ccgcagtaag catctccgcg tcctccgaca agatcattct gagattcttc cgctgctgca
     1681 acaaacatgg acggtccgtc aaaatgcttt gcaagcagtg tctctgaaat ttccggtccg
     1741 aaatttccaa gatatacgca aagtgcatta cagcctgcct tcttgatatc ctcaagtgcc
     1801 tgtaccatat ggatctcact ttctacgata cagatcgggc actcataaat atcagatgca
     1861 tcatactttt tcgtataggc cgcaacaagt gcctttcttc tgtttaccga taatggttcc
     1921 ggaaaacaat cacgggaaac tgccacaata ccgattttta cttttggaat gttattcata
     1981 tttttatcct cctgaaatta attgatcaca tacatactta cttttttcgc agagcacctc
     2041 gaaaccttcg gttcctcggt cgcgttgctc cccggattct tcccggtctg tccttcggta
     2101 caagtacctc ttgacttccc tttcgaatcc ggctcttttc tttcgcagag cacctcgaaa
     2161 ccttcggttc ctcggtcgcg ttgctccccg gattc
//
LOCUS       DAOSYE010000369         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40162_length_2194_cov_3.198691, whole genome shotgun sequence.
ACCESSION   DAOSYE010000369 DAOSYE010000000
VERSION     DAOSYE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40162_length_2194_cov_3.198691"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLU43_06995"
     CDS             complement(<1..1061)
                     /locus_tag="PLU43_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO28195.1"
                     /translation="MQIGFFICNCETAEELIYSGPQLEKIKEAGRGSFLVEKERFGIS
                     ESVEILFTWDGKSVDFLLHEKGSCRFFEDGKIIRNKCFSEQCFFTIVTAKREKFWLSL
                     IFYNTVKAKIYLTKQNVDVGRGAKNTICCVGDRFISDLHLRIRKKEGTAEAAVEGRNG
                     IYINRRYIGRHGKCRLYFGDEICFFSWKIIWLDSLLAVYSGAGQDVSAALLKYTGAES
                     INVLAPAGRKGAGKDKPEQVFSPSPRNYPVLNREAVELEFPPEKREEEKQPLFLTIGP
                     AFTMALPMLAGFGSVILISRSQDNRSGTFLYMGIITAVTSAFLGVFWGIMNVRQRQKR
                     IRQAEGQRQNAYLKYAADC"
     gene            complement(1283..>2194)
                     /locus_tag="PLU43_07000"
     CDS             complement(1283..>2194)
                     /locus_tag="PLU43_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO28196.1"
                     /translation="TDKEGMLTEYQLRYLLMDTSGDHSDSDRTLAFVDVSEQLSDFHK
                     LVLTLVIVGVLCELAFLIISIFLAKFTIKPVRIAWEQQRQFVADASHELKTPLTVILA
                     NVGILLGHKENTIAREEKWVDYIKTEAERMKKLVEDMLFLAKTDMAGLPVERRKINFS
                     DLVMSSLLAFESVAFENGIKLKQEIAQEIALYGNETQLRQMAIIFLDNACKYTPSGGE
                     VMVRLIKKQDKIYFSVKNTGSYIPKEVQDRIFERFYRVDESRVRKEGGYGLGLSIAKS
                     IVDSHKGKLTVSSSEKDGTTFTMQFPQ"
BASE COUNT          615 a          571 c          373 g          635 t
ORIGIN      
        1 tcacaatcgg ctgcatattt caaataagcg ttctgtctct gcccttctgc ctgccgtata
       61 cgtttttgcc tctgccggac attcataatt ccccaaaaaa caccaagaaa cgcagacgta
      121 acagctgtta ttattcccat atacaaaaaa gtaccgcttc tattatcctg ggatcttgaa
      181 atcagaatta ccgagccaaa cccggccagc atcggcaagg ccatcgtaaa tgccggtccg
      241 atcgtcagga acagcggctg tttttcctct tctctttttt ccggaggaaa ctctaactcc
      301 accgcttccc tgttaagaac cgggtaattt cgcggtgacg gagaaaatac ctgttccggc
      361 ttatcctttc cggctccttt ccgaccggcc ggggctaaaa catttatgga ttctgcaccg
      421 gtatatttca aaagagcagc cgatacatcc tggccggccc cggaatacac tgccagcagc
      481 gaatccagcc agatgatctt ccatgaaaag aaacagatct catctccaaa gtacaaccgg
      541 cactttccat gcctgccaat ataacgccgg ttgatataga tcccattcct cccttctact
      601 gccgcctcag ctgttccttc ctttttccgg atccgcagat gcagatctga aataaaacga
      661 tccccaacgc agcatattgt gtttttcgcg cctcttccta catcaacatt ctgttttgtc
      721 aaataaattt tggccttgac agtattataa aaaatgagcg acagccagaa tttttctctt
      781 ttagccgtta caattgtaaa aaaacactgt tctgaaaaac atttgttcct tatgattttt
      841 ccgtcttcaa aaaacctgca gctgcctttt tcatgcagca gaaagtccac actttttcca
      901 tcccatgtga ataggatctc aacagattcc gaaatcccga acctctcttt ttcgacaaga
      961 aaactccctc ttccggcttc tttgattttt tccagctgcg gaccggaata aataagctct
     1021 tccgctgtct cgcaattgca aatgaaaaaa ccgatttgca tacagtcctc ccctgtaatc
     1081 cgatcagcca tcgtcgaatc acaaaaccat aaattcaatg aattgtcttt gtttgaatat
     1141 ttccgtgcga caggcacggt catgaaacga atcgtatttg cttattaatc tgaaatagaa
     1201 ctgcatattc aagcttaatg ttttattatc atatcatgtg ccattttaaa aatcaacaca
     1261 aaataaaaaa atgttcccgc atttactgcg ggaactgcat cgtaaaagtt gtaccatcct
     1321 tctcactgct cgaaacagtc agctttccct tatgagaatc cacaatactt ttggcaatcg
     1381 acaagccaag tccatatccg ccctcttttc tgaccctgga ctcatctacc ctgtaaaaac
     1441 gttcaaatat tctgtcctgt acttctttcg gaatatagga acctgtgttt ttaacggaaa
     1501 aatatatttt atcctgcttt tttataagcc gcaccatgac ctcaccgccg gacggtgtat
     1561 atttacatgc attgtcaaga aaaattatag ccatttgacg aagctgtgtt tcatttccat
     1621 acagcgcaat ttcctgtgca atttcctgtt tcagtttgat cccattctca aaagcaactg
     1681 attcaaacgc aagaagactg ctcatgacaa gatcgctgaa attgattttc cgtctctcga
     1741 caggaagtcc tgccatgtct gtctttgcca gaaaaagcat atcctctaca agctttttca
     1801 tgcgttctgc ctcagtttta atataatcga cccacttttc ctctctggcg atcgtattct
     1861 ccttatggcc gagcaaaata ccaacattgg caaggattac cgtaagaggt gtttttaact
     1921 catgggaagc atccgcaaca aactggcgct gctgttccca tgcgatcctg accggtttga
     1981 tcgtaaattt agcaagaaaa atactgataa tgagaaacgc aagttcacat aagaccccta
     2041 cgatcactaa tgtcaataca agcttatgaa aatcactcag ctgctccgac acatccacaa
     2101 aagcaagcgt acggtccgaa tcagaatggt caccggatgt atccatcagc aaatagcgaa
     2161 gctgatattc cgtcagcatg ccttccttgt ctgt
//
LOCUS       DAOSYE010000370         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40313_length_2189_cov_2.888472, whole genome shotgun sequence.
ACCESSION   DAOSYE010000370 DAOSYE010000000
VERSION     DAOSYE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40313_length_2189_cov_2.888472"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLU43_07005"
     CDS             complement(<1..762)
                     /locus_tag="PLU43_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28197.1"
                     /translation="MTNLVAWIIRAVVFGTVVLYGAIGETITEKVGHLNLGTPGIMCI
                     GGAFGFVGAYSYEIHTTDPNGAVCILIALSMSFFASLLAGLVYSFLTVSLLINQNVVG
                     LTMTIFGVGIGKFFGSYVIPEGAVSTKASFANSIFGAKMPVLSEMGFVGEIVFSYGFM
                     MYLALLLAIITYLFMQKTRVGLNLRSIGENPATADAAGIAVNRYKYFAICIGSGITGL
                     GGVYYVLDYSSATWATAAGDAIESLAWLSVALVIFV"
     gene            complement(764..1855)
                     /locus_tag="PLU43_07010"
     CDS             complement(764..1855)
                     /locus_tag="PLU43_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28198.1"
                     /translation="MNRKEPVIRVMKRDHIENWKGWTIRAVAVVLALLVEALFVFSIL
                     KIPPHRVYSLMWEGAFGNSIYLTDTIFFTAKLLCIAVALAPAFMMRFWNIGGEGQVLA
                     GALATAIVMVYFSSLPAPLLYLLMFESAVIMGALWGLIPAIFKAKWNVNETLFTLMMN
                     YVAIQIVNYFYNKWRGNASALGKLNKGTKAGYLPEILGSDNVLMVGVVILLTVLVFLY
                     LNHTRHGYEIAVVGESKNTAKYAGMDVKRVVMRTMFLSGAVCGICGFMTVAGQDHSVS
                     SSTAGGYGFTAIIVAWLAKFNTLFMVIISLFIVFLDKGTKHIADTYAGFDDSATKIVI
                     GIMLFFVIGSEFFIQYQLHFRGQKEKEEQ"
     gene            complement(1848..>2189)
                     /locus_tag="PLU43_07015"
     CDS             complement(1848..>2189)
                     /locus_tag="PLU43_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28199.1"
                     /translation="TPVRRLSGGNVQKVLVGREIESNPTVLMTAYAVRGLDINTSYTI
                     YRLLNRQKKKGTAVIYVGEDLDVLLALSDRIMVLCGGKVNGILDARSTTKEEVGLLMT
                     KLRESKGAGHE"
BASE COUNT          657 a          600 c          389 g          543 t
ORIGIN      
        1 aacaaatata acaagtgcta ccgacagcca tgccagactc tcgatcgcat ctcctgcagc
       61 ggtcgcccat gtcgcactgc tgtaatccag tacgtaatag acgcctccaa gtcccgtgat
      121 tcccgatcca atacagatcg caaaatattt atagcggttt accgcaattc cggcagcatc
      181 cgcagttgcc ggattttctc cgatcgaacg aagatttaag ccgactcttg tcttctgcat
      241 aaacagatac gtaatgatcg caagcagcag tgcaagatac atcatgaatc cataggaaaa
      301 cacgatctct cccacaaaac ccatttcact caaaaccggc attttcgccc cgaaaatact
      361 gtttgcaaaa gaagcctttg tggaaaccgc accttccggt attacatagg agccaaaaaa
      421 tttaccgatc ccaacaccga agatcgtcat cgtaagtcct actacattct gattgatcaa
      481 aagcgaaacc gttaaaaagg aataaaccag gccggccagc agagaagcaa aaaaactcat
      541 ggataaagca atcagaatac agacagcacc gttcggatct gttgtatgta tctcatagga
      601 ataagctcct acaaatccga aagctccgcc gatacacata ataccgggag ttccaagatt
      661 taaatgtccg actttttccg taatagtctc gccgatcgca ccatatagta caacagtgcc
      721 aaatactacg gcccgtataa tccatgcgac taaattcgtc atattactgt tcctcctttt
      781 ctttctgtcc gcggaaatgc agctgatact ggatgaagaa ctcgctgccg attacaaaaa
      841 acagcataat gccaattacg atcttagtcg cactgtcatc aaatcccgca tatgtatctg
      901 caatatgttt tgttccttta tccagaaata caatgaacag ggaaataatg accatgaaca
      961 gtgtattgaa ttttgccagc caggcaacga tgatcgcagt aaaaccgtaa ccgcccgcag
     1021 tcgaggagga aacggagtga tcctgacccg ccacagtcat aaatccgcag atcccgcaaa
     1081 cagcgccgga taaaaacatc gtacgcataa caacccgttt tacatccatg cccgcatatt
     1141 tggcagtatt cttggattct cccacaacgg cgatctcata tccatgcctt gtatgattca
     1201 gatacagaaa aacaagaacc gtaagcagta ttacgacacc taccatcaat acattatcgc
     1261 ttcccaaaat ttcaggaaga tatccggcct ttgttccttt gttcagtttc ccaagggcag
     1321 aagcatttcc tctccattta ttgtagaaat aattcacgat ctggatcgca acataattca
     1381 tcatcagtgt aaacagtgtc tcattcacat tccactttgc tttaaaaata gccggaatca
     1441 gtccccataa cgcacccatg ataacagcag actcaaacat cagcaaatat aacagcggag
     1501 caggcagact gctgaaatat accattacga tcgcggttgc aagggctcct gcaagcacct
     1561 gtccctcacc gccgatattc cagaaacgca tcataaatgc cggcgccagc gcgactgcaa
     1621 tacacagcag cttcgccgta aaaaatatcg tatcggtcaa ataaatggaa tttccaaaag
     1681 ctccttccca catcagagaa tacacccgat gcggcggaat cttaagaatc gaaaatacaa
     1741 atagcgcctc gacgagcaat gccagtacta ctgcaactgc acgaatcgtc caccccttcc
     1801 agttctcaat atgatctctt ttcattactc gaataacagg ttcttttcta ttcatgtccg
     1861 gctcctttac tttcccgcag tttagtcatc agcaatccca cttcctcttt ggtcgtgctt
     1921 cttgcatcta aaattccgtt tacctttcca ccgcacagta ccattatccg gtccgataaa
     1981 gccagcagca catccaggtc ttccccgaca tagatcactg cagtcccttt tttcttctgc
     2041 cgattcaata aacgatagat cgtataggat gtattgatat ccagtcccct taccgcatag
     2101 gcggtcatca gaactgtcgg attgctttcg atctcccgtc ctaccaatac tttttgaaca
     2161 ttccccccag agaggcgtct gaccggcgt
//
LOCUS       DAOSYE010000371         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40519_length_2182_cov_2.614951, whole genome shotgun sequence.
ACCESSION   DAOSYE010000371 DAOSYE010000000
VERSION     DAOSYE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40519_length_2182_cov_2.614951"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1007)
                     /locus_tag="PLU43_07020"
     CDS             complement(126..1007)
                     /locus_tag="PLU43_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOO28200.1"
                     /translation="MKKECERKKKPIETDAVRLLIVAIAAFVLALNLKSFVWAGSLVP
                     GGLSGVTVLLQRIFKYFFNISIPYTPIYLVFNAGPIILSLRNLGKKFTLYSIFMIVLS
                     SIFTDLLPDITITYQLPLISIFGGLINGAVISACLFANATSGGLDFVAVYLSEKKSVD
                     AWNYILAFNAFAIGICGYLFGWDTALYSIIFQFTSTQVVRTLYRKYKKDTLLIITKKP
                     QEVYESIREATNHDATLFKGEGCYRMEERTMVYSVVAGDEVRGVTDLVKQVDADAFIN
                     VIHTEQIGGRFYRRPNE"
     gene            complement(1024..>2182)
                     /locus_tag="PLU43_07025"
     CDS             complement(1024..>2182)
                     /locus_tag="PLU43_07025"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOO28201.1"
                     /translation="KWYYAVPIVVIWLLVVIAVIRIICSAAGVFSQQAELPEGTQLGA
                     AAISEGMPKEGYRYLYFGENEGIPIEWRFLCADTSMGGEDGIFLMANRGMAFSDFGTD
                     NKWEHSKIKDWCVDFYESDTHFTSAEKKLIMETTVEETEQDDYHYNDGSRFLHCSLEK
                     EHIFILSAAEAMNPMFGFSESIRTDENREMQMGTRLRWWWLRSPDSKSDQIGCLIYRD
                     GNITYDIVTRSMIGIRPVMNLLTDSSSGIFMAVPCEEYVVQEKNWVDKTQALNGQSAY
                     WKLKLSDDTIRTPQFEDAGVRSGKLKVIFSDGKPVLEEGQCLMLLITDKQYRTAYAYI
                     PVQDSFYRSDGTLEWELPSYYNKKKCDLYLLAEMEGEAQQTDYVSLPAKLE"
BASE COUNT          591 a          603 c          413 g          575 t
ORIGIN      
        1 ggtctcccac cagaacctaa atctggcgcg tctgccaatt ccgccatatc cgcatatgac
       61 taacatccca tatgttatca tacgcctaag ggaatgtcaa tttgatctgc ccgcaaaaca
      121 acgcattatt catttggacg gcggtaaaat cttccgccga tctgctccgt atggatcaca
      181 ttgataaaag catctgcatc tacctgcttt acaagatccg taactccccg cacctcatct
      241 ccggccacta cggaatagac cattgtccgc tcctccatgc gataacagcc ctctcccttg
      301 aacagcgttg catcgtggtt cgtggcttcc ctgatacttt cataaacctc ctgcggtttt
      361 tttgtaatga tcagcagcgt atccttctta tatttacgat acagtgtcct gacgacctgt
      421 gtcgaagtga actgaaaaat gatcgaataa agtgcggtgt cccagccgaa cagataaccg
      481 caaatcccga tcgcaaaagc attaaatgca agaatataat tccaggcgtc aacacttttc
      541 ttttccgaaa ggtaaacggc aacgaaatcc aggccgccgc ttgtagcatt ggcaaaaaga
      601 cacgccgaaa taacggcacc attgatcagg ccgccgaaaa tactgatcag cgggagctga
      661 taagtgatcg tgatatcagg cagcagatct gtaaaaatac tgctgagaac gatcatgaaa
      721 atggaataca gcgtaaattt tttcccaaga tttctaaggc ttaaaataat cggtccggca
      781 ttgaacacaa ggtagatcgg cgtatatgga atactgatat taaagaaata tttaaagatc
      841 cgctgcagca gaaccgtaac accgcttaat ccgcccggca ccagactccc cgcccataca
      901 aagctcttca ggttcagtgc aagcacaaac gccgcaattg ccacgatcag cagcctgacc
      961 gcatctgttt ctattggttt cttttttcgt tcgcattctt ttttcatttt actattccta
     1021 tactcattca agtttggcag gaaggctcac atagtccgtc tgctgcgctt ccccttccat
     1081 ctcggccagc agatacagat cgcatttctt cttattataa taggacggca gctcccactc
     1141 cagtgtaccg tcggatctgt aaaaggaatc ctgtaccgga atatatgcat atgccgtccg
     1201 gtactgcttg tccgtaatca gcagcatcag acactgccct tcctcaagga ccggcttccc
     1261 gtcggaaaat atcaccttca gtttcccgga acgtacgccg gcgtcttcaa actgcggcgt
     1321 cctgatcgta tcatccgata acttcagctt ccaataggcc gactgtccat ttaaagcctg
     1381 cgtcttatcc acccagttct tctcctgcac aacatactct tcacacggta ccgccataaa
     1441 gattccggaa ctgctgtctg ttagcagatt cataaccgga cgtatcccga tcatggagcg
     1501 tgtcacaata tcataggtaa tatttccatc cctgtagatc agacagccga tctgatccga
     1561 ttttgaatcc ggagaacgga gccaccacca gcgcaggcgc gttcccatct gcatctcacg
     1621 gttttcatcc gttcggatcg attctgaaaa accaaacatc ggattcatag cctcagcagc
     1681 ggacagtata aaaatatgtt ctttttcaag actgcagtgc agaaaccggc ttccatcgtt
     1741 ataatggtag tcatcctgtt ctgtctcttc aaccgttgtc tccatgatca attttttctc
     1801 agctgatgta aaatgcgtat ctgattcata aaaatcaaca caccagtctt ttattttgct
     1861 atgctcccat ttattatccg taccaaaatc agaaaatgcc attccccggt ttgccatgag
     1921 aaagatgccg tcttctcctc ccatactcgt atctgcgcaa agaaatctcc attcgatcgg
     1981 tattccttca ttttctccaa aatagagata acgatatccc tcttttggca tcccttcact
     2041 gatggccgca gccccaagct gtgttccttc cggaagttca gcctgctgtg agaaaacacc
     2101 cgcagcagaa cagataatac gaatgaccgc gatcacaaca agcagccaga tcacaacgat
     2161 cggaaccgca taataccact tc
//
LOCUS       DAOSYE010000372         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40751_length_2174_cov_2.967437, whole genome shotgun sequence.
ACCESSION   DAOSYE010000372 DAOSYE010000000
VERSION     DAOSYE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40751_length_2174_cov_2.967437"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="glpK"
                     /locus_tag="PLU43_07030"
     CDS             <1..1070
                     /gene="glpK"
                     /locus_tag="PLU43_07030"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOO28202.1"
                     /translation="DHVPGARKRAEARELLFGTVETWLIWKLTGGAVHVTDYSNASRT
                     MLFNIRTLDWDPDILEELSIPRSMLPKACSSSSIYGMTDESFLGGKIPIAGAAGDQQA
                     ALFGQTCFREGEAKNTYGTGCFLLMNTGETPVYSKNGLVTTIAWGLDGKINYALEGSI
                     FVAGAAIQWLRDEMRLIDSAEDSAYMAEKVPDTNGCYVVPAFTGLGAPYWNQYARGTI
                     VGLTRGVNKYHVIRATLESIAYSADEVVSAMEADSGIVLTSLKADGGASANDFLMQCQ
                     ADISDVPVKRPACVETTAMGAAYLAGLAVGYWKGTEEILANRQTDRTFVKRITDSERM
                     AKKKGWLKAVRYAFDWAKEDK"
     gene            1147..1845
                     /locus_tag="PLU43_07035"
     CDS             1147..1845
                     /locus_tag="PLU43_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOO28203.1"
                     /translation="MYSFNSRIRYSEVDEDKNLTVGGIINYFQDCSSFQSEDIGVGFD
                     FLESNHRAWVLNAWQIVLEKYPKYGERITIGTWPYDFRGFIGYRNFVLLNEEKEKLAY
                     ANSIWSYLDTQTGRFAKAGEYELSCYRLEERLDMEYADRKIRIPEGGENAEGVVITEH
                     QIDTNHHVNNEEYVKLAMDILRIKNPRQVRVEYKKSALLGNVIYPRVFTERSEKTVVL
                     ADEDGSPYSVVVMS"
     gene            1886..>2174
                     /locus_tag="PLU43_07040"
     CDS             1886..>2174
                     /locus_tag="PLU43_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOO28204.1"
                     /translation="MLELGKEQELTIVKKVEYGVYLAESADDEIRVLLPVKQVPEGAV
                     IGGKLSVFLYLDSKDRLIATTRMPKIRLGETAVLKVVQTGKIGAFVDWGLEK"
BASE COUNT          625 a          394 c          637 g          518 t
ORIGIN      
        1 tggatcatgt accgggtgca agaaaaaggg ctgaggcaag agagctgctg tttgggacgg
       61 tagaaacatg gctgatctgg aaactgacag gaggcgctgt tcacgttacg gattattcta
      121 acgcgtcgcg aacgatgttg ttcaatatcc gtacacttga ctgggatcct gatattctgg
      181 aagaactctc cataccccgc agcatgctgc caaaggcctg ttcatcaagc agcatttatg
      241 gaatgacaga cgaatcgttt ttgggaggta aaataccaat tgcaggggca gcaggcgatc
      301 agcaggccgc acttttcggg cagacctgtt ttcgcgaggg agaagcaaaa aacacatatg
      361 gaaccggctg ctttcttttg atgaatacag gagaaacacc ggtctattct aaaaacggac
      421 ttgttacgac gattgcatgg ggacttgacg gaaaaataaa ttacgctttg gaaggttcta
      481 tctttgtggc gggagctgcc attcaatggc tgcgcgacga gatgcggctg atagacagtg
      541 ctgaggattc cgcctacatg gctgaaaagg tgccggatac aaacggctgc tatgtggtgc
      601 ctgcttttac gggactggga gcgccgtatt ggaatcagta tgcaagaggt acgatcgtcg
      661 gtttgacgag gggtgtcaat aaatatcatg ttattcgtgc aacgctggaa tcgatcgctt
      721 attctgcgga tgaagtcgtc agcgccatgg aagcggattc cggtattgtg ctgacatcgc
      781 tgaaagcaga cggcggcgca agcgccaatg attttttgat gcagtgccag gcagatattt
      841 cagatgtacc ggtcaaacgg ccggcatgtg tggaaacgac ggcaatgggc gccgcctatt
      901 tggcgggtct tgcagttggt tattggaaag ggacggaaga aattcttgca aaccggcaga
      961 ccgacaggac atttgttaaa aggatcacgg acagcgaacg gatggcaaag aagaaaggct
     1021 ggctgaaagc tgtgagatat gcgtttgact gggctaaaga ggataaataa ggaagaaatc
     1081 cgtcgtgcaa tttaggcgga aatatgttat agtattgaaa acaggattgt cgggagatta
     1141 tggaaaatgt acagttttaa ttcaagaatc agatacagcg aggtagatga agacaaaaac
     1201 ctgacagttg gcggaatcat caattatttt caggactgca gttcgtttca gtcggaagat
     1261 atcggagtcg gttttgattt cctggagagt aatcacaggg catgggtttt gaatgcatgg
     1321 cagatcgtgc ttgaaaagta cccgaaatac ggagaacgga taacgatcgg aacgtggccg
     1381 tatgatttca ggggatttat cggttaccga aattttgtgc ttttgaatga agaaaaagaa
     1441 aagctggctt atgcaaactc gatctggtcg tatttggaca cgcagacggg caggtttgca
     1501 aaggcggggg aatatgagct ctcctgttac cggcttgagg aaaggctgga catggaatat
     1561 gccgaccgta agatcagaat cccggaggga ggagaaaatg cggaaggcgt cgtgatcaca
     1621 gaacaccaga tcgatacaaa tcatcatgtc aataacgagg aatatgtgaa actggcaatg
     1681 gatattttaa ggatcaaaaa tccgcgtcag gtccgcgtag agtataaaaa atcggcgctg
     1741 cttggaaatg ttatttatcc aagagtattt acggaaagat cagaaaaaac agttgtgctt
     1801 gcagatgaag atggaagtcc gtattctgtt gttgtaatgt cctgacagcg gccggattgt
     1861 tttatccggc ggataaggag caattatgtt ggaacttgga aaagagcagg agctcacgat
     1921 tgtaaaaaaa gtagaatatg gagtttatct ggcggagtcg gcagatgatg agattcgtgt
     1981 actgcttccg gtcaaacagg taccggaggg agcagtaatc ggcgggaagc tttccgtatt
     2041 tctttatttg gattcgaaag accgcctgat cgcaacgact cggatgccga agatccggct
     2101 gggggaaaca gcggtgctga aagttgtgca gacaggaaaa atcggagcct ttgtagactg
     2161 gggtctggaa aaag
//
LOCUS       DAOSYE010000373         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_40795_length_2172_cov_3.586679, whole genome shotgun sequence.
ACCESSION   DAOSYE010000373 DAOSYE010000000
VERSION     DAOSYE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40795_length_2172_cov_3.586679"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLU43_07045"
     CDS             complement(<1..1082)
                     /locus_tag="PLU43_07045"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28205.1"
                     /translation="MKKKPGFFEKLSYRFDNLMSKGTAALVGMLFLITFVVVIITGIL
                     GSLCNGDLTTGNSIWQSLMHALDAGTLAGDTTANIPFIILMTVVTVCGIFVTSILIGI
                     ISTGFEEKLNSLRKGLSRVLETEHTVIVGFNDEIYTILTELIEANSNHKNACIVILDE
                     ADKEEMDEEIRSHISDFKTTRIICRSGSCTNKHSFHLISVETARSVIVNREDDFQVIK
                     SILALTSYLKETGAFTNSAHITALIHHKENLDAAKIAAEGKAEIIFFKDILARIVAQV
                     CRQPGLSLVLSDFFSYGGSEFYYEKFPELSGKAFGDILGLFENSVVVGIKKNGQTLLN
                     PPIDMIINKDDFIIHLAEDDGVSKPAV"
     gene            complement(1177..2055)
                     /locus_tag="PLU43_07050"
     CDS             complement(1177..2055)
                     /locus_tag="PLU43_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO28206.1"
                     /translation="MLDFRIETFLCVCKHMNYTKAAEELHITQPCITQHIRFLEEYYN
                     TKLFRYQNRTLRLTPAGKKLLSGMSSMKHDTDHLKASVASADSRTELYKMGATRTIGE
                     YYLPKKLLKWLKERPDLSIELSISNTQKLLELLDNGDIDFALVEGYFEKNNYDFITAE
                     IENFIPVCGINFPITALKDFSDILEFPLLLREKGSGTREILERFLALKGLAISSFSQV
                     HTISSIPVIKQMLEQNLGISFLFETAVSEELQHMTLKKIDLPGFLLSHEFTFIWRKNS
                     IFQDTYQSVCNSLLGR"
BASE COUNT          639 a          432 c          438 g          663 t
ORIGIN      
        1 acagcgggct ttgaaacacc gtcgtcttct gccagatgaa tgataaaatc atctttgttt
       61 atgatcatgt cgatcggagg attcaacaag gtctgtccgt tttttttgat accgaccacg
      121 acggaatttt caaacagtcc caaaatatca ccaaaagctt ttccagataa ttccggaaat
      181 ttctcataat aaaattcgga gccgccatag ctgaaaaaat cggacagaac gagtgaaagt
      241 cccggctggc ggcagacctg tgcaacgatt ctggctaaaa tatccttgaa aaaaatgatt
      301 tcagcctttc cttccgcagc aattttagca gcgtccagat tttctttatg atggataaga
      361 gcggtaatat gagcgctgtt cgtaaaggca cccgtttctt tcagatagga agtcagcgcc
      421 agaatggact ttatgacctg aaagtcgtct tcccggttta ctatgacaga tctggcagtt
      481 tcaacagata taagatggaa ggaatgtttg tttgtgcagc tgccggaacg gcagatgatt
      541 ctggttgttt taaaatcaga aatatgtgaa cggatttctt catccatttc ttctttatcg
      601 gcttcgtcca gtattacgat acaggcattt ttatgattgg agtttgcttc gatcaattct
      661 gtcaggatcg tatagatctc atcgttaaaa ccgacaatga cagtatgttc cgtttccagg
      721 acgcgggaaa gacctttgcg caggctgttc agtttttctt caaaaccggt gctgataatg
      781 ccgatcagga tacttgttac gaaaataccg cagaccgtta cgactgtcat taaaatgata
      841 aacgggatat ttgccgtagt atctccggcg agagttcctg catcaagggc atgcatcaga
      901 ctctgccaaa tggaattgcc tgttgtcaga tcgccattgc acaaactgcc aagaattcct
      961 gttatgatta caacaacaaa cgtaattaaa aagagcatac cgacaagggc ggcagttcct
     1021 tttgacatca ggttatcgaa acgataactt aatttttcaa aaaatccagg tttttttttc
     1081 ataataaaat tcctccgcac cagttattct gataagatcg ttatccttat cataatgaga
     1141 aaagaataaa aatgcaataa aaaataaatg gtttgttcaa cggccgagaa gagaattgca
     1201 gacagattgg taagtatcct gaaaaatact gttttttctc cagataaaag tgaattcatg
     1261 ggaaagcagg aatcctggaa gatctatttt tttcagcgtc atatgctgca gttcttcact
     1321 cacggcggtt tcaaacaaaa acgaaattcc aaggttctgt tccagcatct gcttgatgac
     1381 cgggatactg ctgatcgtat ggacctgaga aaaggagctg atcgcaagtc ctttcagtgc
     1441 cagaaaccgt tccagtattt ctcttgtccc ggaccctttt tcccgaagca aaagtggaaa
     1501 ttccaaaatg tcgctaaaat ctttcaaggc ggttatagga aaattgattc cgcatacggg
     1561 aataaaattt tctatttcag ccgtaataaa atcatagtta ttcttttcaa aatatccttc
     1621 cacaagtgca aagtctatat cgccgttgtc cagcagctct aacagcttct gcgtattgga
     1681 gatgctgagt tcaatggaca gatcagggcg ttctttcagc cattttaaca gtttttttgg
     1741 aagataatat tctccgatcg tccgggtagc ccccatttta tataattcag tcctggaatc
     1801 tgcagatgcc acagaagctt ttaaatgatc ggtatcatgt ttcatggagc tcatgccaga
     1861 taatagtttt ttccccgcag gcgttaagcg cagcgtccgg ttttggtagc ggaaaagttt
     1921 tgtattataa tattcctcca gaaaacgaat gtgctgcgtg atacagggct gtgtaatgtg
     1981 gagttcttct gcggctttcg tataattcat gtgtttgcag acgcatagaa acgtttcgat
     2041 tctgaaatca agcataggtc gtctccaatt tctttcttta tatataataa atatttatct
     2101 atatataata ataaattaac ctgaattatt catggtgatg taaaaaaaat attttgctcg
     2161 ccaatttact tt
//
LOCUS       DAOSYE010000374         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41010_length_2165_cov_2.383886, whole genome shotgun sequence.
ACCESSION   DAOSYE010000374 DAOSYE010000000
VERSION     DAOSYE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41010_length_2165_cov_2.383886"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLU43_07055"
     CDS             <1..519
                     /locus_tag="PLU43_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28207.1"
                     /translation="VYYYLGTYIKEYRPKVKKWLLVLITVVIVVGEAVTSYLFADGKS
                     FIWNVLGPVDSGYSTITVVISAAAVFLLFYDVKLKTNWLKVVFRKISEVSFEIYLFTG
                     VYDAIVFYYLKRIIWEVEDFFWWFFITVPVSFLLAWFSSMLLKLIFKEVKKYVLLPIY
                     RRAAEKKKEKAS"
     gene            complement(527..1144)
                     /locus_tag="PLU43_07060"
     CDS             complement(527..1144)
                     /locus_tag="PLU43_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28208.1"
                     /translation="MKQYRHKLNTLHGGGAVLPASCICLCIGLGMQALSLPVLGTVFF
                     ILSALLFAALMILLIIEQHQDDVLNREAMQADEKLEAAIKKKSFSLPYHNGEIWCEHL
                     DGLYYYTELVIHKFQNDLARLQSPSAPSAIAINLDETAINDELLHILCSGLCSHKKHL
                     QKIAFIGISKKDRPKMKLHLINASVPFQYRFFHDFEKAKEWLISI"
     gene            1319..1403
                     /locus_tag="PLU43_07065"
     tRNA            1319..1403
                     /locus_tag="PLU43_07065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1353..1355,aa:Leu,seq:aag)
     gene            1483..2055
                     /locus_tag="PLU43_07070"
     CDS             1483..2055
                     /locus_tag="PLU43_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO28209.1"
                     /translation="MSKIAIYFPGIGYTADKPLLYYGRKAAYEAGFHEYRNVTYIYTR
                     KDNLRGNDEKMKEVYESLFPQAESQLTDIDWNAYDDVLFVSKSIGTIVAASYAKQYEL
                     KQVSHILYTPLARTFDYTLKEAIGFIGTADPWSNVDEIIKLSEKAGIPLNIYDNCNHS
                     LECDDTFRNLEVLRNVMEKTFSFCRKSSCI"
BASE COUNT          659 a          361 c          475 g          670 t
ORIGIN      
        1 gtctattatt atctggggac atatatcaaa gaataccgtc cgaaagtgaa aaaatggctg
       61 cttgtgctga tcacagtcgt tattgtagtg ggagaggcag ttacgtccta tttgtttgct
      121 gacggaaaga gttttatatg gaatgtgctc ggcccggtgg acagcggtta ttcgacaatt
      181 actgttgtta tttccgctgc tgcggtattt ctgctttttt atgatgttaa gcttaagacg
      241 aactggctca aagtagtctt tagaaaaata tcggaggttt cttttgaaat ttatttattt
      301 acaggcgttt acgatgcgat cgtattctat tacctgaaac ggatcatatg ggaagtggaa
      361 gactttttct ggtggttttt tataaccgta ccggtgagtt ttcttttggc atggttttct
      421 tccatgttat tgaaattgat tttcaaagaa gtaaaaaaat atgttttgct gccaatatat
      481 agacgggcag cggaaaaaaa gaaagaaaag gcaagttagc agccgcttaa attgagataa
      541 gccattcctt tgccttttca aaatcatgaa aaaaccggta ctgaaacgga acagatgcgt
      601 ttataagatg cagtttcatt ttgggccggt ctttttttga aataccgata aaagctattt
      661 tctgcaaatg ttttttatga ctgcacagtc cgctgcagag gatgtgcaga agttcgtcat
      721 tgatcgcagt ttcatccaga ttgatcgcga tggccgatgg tgcggaagga gactgcagtc
      781 tggccagatc attctgaaat ttatgaatta ccagttcggt ataataataa agtccgtcca
      841 gatgttcaca ccagatctca ccgttatgat atggaaggga aaaggatttc ttttttatgg
      901 cagcttccag cttctcatct gcctgcatgg cttcccgatt caatacatca tcctgatgct
      961 gctctattat gagcaatatc ataagcgctg caaacaggag tgcagataaa ataaagaaaa
     1021 cggttccaag aacaggcagg gacagtgcct gcatgccaag tccgatgcac agacagatac
     1081 agcttgcggg cagtacggcg cctccgccgt gtaatgtatt taatttatga cgatactgtt
     1141 tcatttaccg attctccaat tttgaataat tttgctgttc tgcctattat aaaggaaaca
     1201 gaaggacatt tctacttaca aatgtcatca ttttaacggg tgtattcaaa ttaatttttg
     1261 aaattaggga ttgattttta cataagaaaa tgttattata tcaaagtcgc gatgacatgc
     1321 ggaagtgtcg gaattggcag acgagcaaga ctaaggatct tgtgatcaat gcgatcgtgt
     1381 gggttcaagt cccatcttcc gcactagagt aagaggcctg gaaacattga gaaatcaatg
     1441 gttctgggtt ttctttttgg aaataattct gaggtgatat aaatgtctaa aatagcaatt
     1501 tattttccgg gaataggata cactgcggat aaaccacttt tatattatgg aaggaaggct
     1561 gcttatgaag ccggattcca tgagtataga aatgtaacct atatatatac gcgaaaagat
     1621 aatttacgcg gaaatgatga aaaaatgaaa gaagtatacg agtcactgtt tccacaggca
     1681 gagagtcagc tgacggatat tgattggaat gcttatgatg atgtattgtt tgtatctaaa
     1741 agtatcggaa ctattgttgc agcttcttat gcgaaacaat atgaattaaa gcaggtcagc
     1801 catatcttat acacgccgct tgcccggacc tttgattata cgcttaagga agccattggc
     1861 tttatcggaa cagcagatcc atggagtaat gtggacgaaa taattaagct atcagaaaaa
     1921 gccggtattc cattgaatat atatgataac tgcaatcatt ctctcgagtg tgatgatacg
     1981 tttagaaatt tagaagtatt aaggaacgta atggaaaaga cgtttagttt ttgcaggaaa
     2041 tccagttgca tttgaacaca tatatcaaga tacatccttc tttcgttgat tactggaatt
     2101 gttttttaaa cattcaaatg ggcggacgcc tgaatcgcaa acataagcac tgtaatgata
     2161 ttaaa
//
LOCUS       DAOSYE010000375         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41150_length_2160_cov_3.161520, whole genome shotgun sequence.
ACCESSION   DAOSYE010000375 DAOSYE010000000
VERSION     DAOSYE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41150_length_2160_cov_3.161520"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLU43_07075"
     CDS             <1..408
                     /locus_tag="PLU43_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO28210.1"
                     /translation="PTPEEIAVEMNLPVERVREILKISQEPVSLETPIGEEEDSHLGD
                     FIQDDNVPVPADAAAFTLLKEQLVEVLSTLTEREQKVLRLRFGLDDGRARTLEEVGKE
                     FNVTRERIRQIEAKALRKLRHPSRSRKLKDYLD"
     gene            428..1147
                     /locus_tag="PLU43_07080"
     CDS             428..1147
                     /locus_tag="PLU43_07080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO28211.1"
                     /translation="MTILSERLHNVAELVTPGLSAADIGCDHGYLSIYLVENKLAEHV
                     IATDINQGPLRRAKENIRKAGLSEMIETRLSDGLKKLAPGEADCLIMAGMGGRLMSDI
                     IRTSPAVCEKAAEIILQPQSEIAGVRHFLEESGYLIISESMVYEDGKYYQMMKAVHGA
                     MHLEREIYYKYGKILLHEENPVLHQFLVQEKKYYVDLYKELAAQDPTTRVSDRLIEVE
                     KELEIVREALNRSKESPYEMP"
     gene            1134..1916
                     /locus_tag="PLU43_07085"
     CDS             1134..1916
                     /locus_tag="PLU43_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOO28212.1"
                     /translation="MKCHEIIAKFEELSPPSYAADWDNVGLLIGRREKEIRKVYIALD
                     ATDEVIRNAVELQADMLLTHHPLIFSPMKKISSEHFIGRRVLRLARHDIAYYAMHTNF
                     DVMGMADAAADEIGLKKREVLEITFEDEIAKEGFGRIGKLPRIMSLSECAEYVKKCFH
                     IEHVTVFGDPDMIVETAAICPGSGKSMIENAIAASADVFITGDIGHHDGIDANAQGLA
                     IIDASHFGIEKIFVPYMKEFLRREFTELTVFTHHPEAPFTVL"
     gene            1945..>2160
                     /locus_tag="PLU43_07090"
     CDS             1945..>2160
                     /locus_tag="PLU43_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOO28213.1"
                     /translation="MEINMVKVTAAGITKEYRTGTVYEEIAKEHQKDFDAKIVLVLVN
                     GKLNELHKKLTGDCTIEFKTVKDRSGYK"
BASE COUNT          636 a          393 c          629 g          502 t
ORIGIN      
        1 ccgaccccgg aggagatcgc agttgagatg aatcttccgg ttgaaagagt acgtgaaatt
       61 ttaaagatct cccaggaacc ggtctcactt gaaacgccga tcggagaaga agaggacagt
      121 catctcggcg attttattca ggatgataat gtgcctgtac cggctgatgc cgcagcgttt
      181 acgttactga aggaacagct tgttgaagtg ctcagcactt tgacggagag ggagcagaaa
      241 gtactgcgtc tgcgttttgg tctggatgac ggacgtgcgc ggacactcga agaggtaggc
      301 aaggaattta atgtaacgcg tgagcgtatc cggcagatag aggcaaaggc actgcgcaag
      361 ctgcggcacc cgagcagaag cagaaagctg aaggattatt tggactgact atataccgga
      421 ggagattatg acgatacttt cggaaagact gcataatgtg gcggaacttg taacacctgg
      481 tctgtcagcg gctgatatcg gctgtgacca tggatatctg tcgatctatt tagtggaaaa
      541 caagcttgca gagcatgtga tcgcaacgga tatcaaccag ggaccgcttc ggcgggcaaa
      601 agagaatatc agaaaggccg gtttgtcgga aatgatcgag acccgtctgt cggacggctt
      661 aaaaaagctt gctccgggag aagcggactg tctgatcatg gcgggaatgg gcggccgtct
      721 gatgagtgat attataagga cctcgcccgc agtctgtgaa aaagcagcgg aaattatttt
      781 gcagccgcag tctgaaattg caggggtcag acattttctg gaggagtccg ggtatctgat
      841 catttcggaa tccatggtgt atgaggacgg gaaatattat cagatgatga aagctgttca
      901 cggagcaatg catcttgaaa gagaaattta ctataaatac gggaaaatat tgctgcatga
      961 agagaatccg gtattgcatc agtttctggt gcaggagaaa aagtattatg ttgacttata
     1021 taaggaactt gcggcgcagg atccaacgac acgtgtaagc gacagactga ttgaagtgga
     1081 aaaggagctt gagattgtca gggaggcgct gaaccgatca aaggagagtc catatgaaat
     1141 gccatgaaat cattgcaaag tttgaagagc tttcgccgcc gtcttatgct gcggactggg
     1201 ataatgtcgg actgctcata ggcagaaggg aaaaggagat ccgaaaagta tatattgcgc
     1261 tggacgcgac ggatgaagtg atccggaatg cggtcgaact gcaggcagat atgcttctga
     1321 cacatcaccc gcttattttt tcaccaatga aaaagataag ttcggagcat ttcatcggac
     1381 gcagggtcct gcggctggcc aggcatgaca ttgcctatta tgcgatgcat acgaattttg
     1441 atgtgatggg aatggcggat gcggctgcag atgagatcgg actgaagaaa agagaggtat
     1501 tggaaattac ctttgaagat gaaattgcaa aagaaggatt tggcaggatc ggaaagctgc
     1561 ctcgtatcat gagtctttca gagtgtgcgg aatacgtaaa gaaatgtttt catatcgagc
     1621 atgtaactgt gtttggggat ccggatatga tcgtagagac tgcggctatt tgtccgggct
     1681 ccggcaagag catgatcgaa aatgcgatag ctgcttcagc ggatgttttt attacgggcg
     1741 atatcgggca tcatgacggg atcgacgcaa atgcgcaggg acttgcaatc attgatgcaa
     1801 gccattttgg aatcgaaaag atatttgttc cttatatgaa agaatttttg cggagggaat
     1861 ttacggagct gacggtattt acgcaccatc cggaagcgcc gtttacggta ttataaaata
     1921 agaaacgggt aattggaggg gaacatggaa atcaacatgg taaaggtaac ggctgccggt
     1981 atcacgaagg agtaccggac aggaactgtt tacgaagaga ttgcaaaaga acaccagaag
     2041 gattttgacg caaagatcgt acttgtcctg gtgaatggaa agctgaatga actgcataag
     2101 aagctgacag gagactgtac gatcgaattc aagacagtga aggacaggag cggatataag
//
LOCUS       DAOSYE010000376         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41151_length_2160_cov_3.073634, whole genome shotgun sequence.
ACCESSION   DAOSYE010000376 DAOSYE010000000
VERSION     DAOSYE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41151_length_2160_cov_3.073634"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..530
                     /locus_tag="PLU43_07095"
     CDS             276..530
                     /locus_tag="PLU43_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28214.1"
                     /translation="MKKKASAWTKEVKKAMIDRDLDLHALCSAIGGYTREYISAVINQ
                     RIYAPDVIKKINTYLQIDEYLENQTLDSGNMENKEEISNL"
     gene            632..1789
                     /locus_tag="PLU43_07100"
     CDS             632..1789
                     /locus_tag="PLU43_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO28215.1"
                     /translation="MKDLKMRKKLLVLATVVGFIPMIVVGLLSYILADRELESAVDTT
                     NSVYALLAEEQLDTYFSERKGNADTLSTADNLVLNMEIYNDAGTSESEKAAALAEMET
                     YLAEEREAYEYPDIFMTDASGTGILAVVYKDELEGADLSERTYVNGALQGTQLWSELF
                     YSSFIDDNAMVLSSPVYNEDGSQVLGTVNLMIDQAKLDEIIHKGADILGETGDSYLID
                     SAGLLLSNTRLGDYVEGAALNQTINTEAAEVLSDEIAAGNTDFTYTGIYDDYRGNAVC
                     GSLGVIKVGDTNAGLIIEIDKSEAFAGVYTLRNVSIIIVLFFYITAVIMLILISSSIT
                     KPLTAVVGNAELLGCAELPSALLFIKLKAKFNIRNLLRTWPVDITAGNWMR"
     gene            1777..>2160
                     /locus_tag="PLU43_07105"
     CDS             1777..>2160
                     /locus_tag="PLU43_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28216.1"
                     /translation="MDAIVMRLWDAILSFPSLLLAFIFVAVFGKSSINAVMALGVVYI
                     PMISKLSRSMTMTEITKTYVEAAHSLGYSDRRIIFVHILPNCIGTMLAELTLDVGSAI
                     MGLASLSFLGLGVQPPTPTWGGMISD"
BASE COUNT          642 a          360 c          537 g          621 t
ORIGIN      
        1 ggtattgaat tttcaaggtg catggctaaa atctatgtgc gaagtttgag taattaagaa
       61 tataatgtat atctttatac tataatttaa acctacccaa agaaatacgc aaataaaagt
      121 ctgctgagat gataccgcag ataaaggaag actggccggt cttgtatatt tcgtgttttt
      181 ttggagggtt taaattagtt attatatttg tattttaagc gatttgggtt tgcttgtaaa
      241 ttgttatttg tgtggtttca taaaagaggg gaaaaatgaa gaaaaaagca tcggcatgga
      301 cgaaagaggt aaaaaaagca atgattgaca gagatctgga tctgcatgca ttatgttctg
      361 cgataggcgg ttacaccaga gagtatatat cagcagtgat caatcagaga atttatgcgc
      421 ctgatgtgat caaaaaaatt aacacctatc tgcaaatcga cgaatatctg gaaaatcaga
      481 cgctggattc aggaaatatg gaaaacaagg aagaaatatc caatttataa tcgataatct
      541 gccggcggag aaccagcggc ttacaatatt taattttatt ttttaacaaa atgagggaaa
      601 gtcaaaatat ttgacgggaa ggaaagaacg tatgaaagat ttaaaaatga gaaaaaagtt
      661 attggtttta gctactgtag taggttttat tccgatgatc gtagtaggtc tgctgtcgta
      721 tattctggca gacagagagc tggaaagtgc cgttgacacg acgaattccg tatatgccct
      781 gctggcagaa gagcagctgg atacttattt ttcggaacgg aagggaaatg cagatacgct
      841 ttccacagcg gacaatcttg tattaaatat ggagatctat aacgacgcgg ggacatcgga
      901 aagtgaaaag gctgctgcac ttgctgaaat ggaaacctat ctggcagagg aaagagaagc
      961 atatgaatat cctgatattt ttatgacaga tgcgagcggt acgggtattc ttgctgttgt
     1021 atataaggat gagctggaag gagccgatct gagtgaacgc acttacgtta atggtgcatt
     1081 acagggaaca cagctgtggt ctgagctctt ttactcaagc tttattgatg ataacgcgat
     1141 ggtgctttcc agtcctgtgt ataatgagga tggcagtcag gtgcttggaa cggtcaatct
     1201 gatgatcgat caggcaaagc tggatgagat catccataag ggagcggata ttctagggga
     1261 aaccggagac tcttacttaa tcgattcagc tggtcttctg ctttccaata caaggctcgg
     1321 ggattatgtg gaaggagctg cactcaatca gacgattaat acagaagcag cggaagtcct
     1381 atcggatgag atcgcagcag gaaatacgga ttttacttat acgggaatct acgatgatta
     1441 cagaggaaat gcggtatgcg gttcccttgg tgtcataaag gtcggagaca caaatgcggg
     1501 actgatcatt gagatcgata aatcagaagc ctttgcaggt gtttatacgc tgcgtaatgt
     1561 ttcgatcatt attgtactgt tcttttatat aaccgcagtc attatgctga ttctgatatc
     1621 ttcctccatt acaaagccgt taaccgcagt tgttggaaat gcggagcttc tgggatgcgc
     1681 cgagctgccg tcggcattat tgtttataaa attaaaggcc aaattcaata tcagaaattt
     1741 gctgagaaca tggcctgtgg atattacggc gggaaattgg atgcgatagt tatgagattg
     1801 tgggatgcga tcctttcttt tccatcactg ctgctggcat ttatttttgt ggcggtattt
     1861 ggtaagagtt ccattaatgc ggtcatggcg ttaggcgtag tttatattcc gatgatctca
     1921 aagctttcca gatcaatgac gatgaccgag ataacaaaaa cttacgtgga agcagcgcat
     1981 tccctcggtt attccgacag gagaatcatt tttgttcata tattgccaaa ttgtatcggt
     2041 acaatgctgg ctgaactgac gctggatgtc ggatctgcaa ttatgggtct tgcatcattg
     2101 agttttcttg gcctgggcgt tcagccgcca actccgacat ggggcggcat gatcagcgac
//
LOCUS       DAOSYE010000377         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41377_length_2153_cov_3.087226, whole genome shotgun sequence.
ACCESSION   DAOSYE010000377 DAOSYE010000000
VERSION     DAOSYE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41377_length_2153_cov_3.087226"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLU43_07110"
     CDS             <1..636
                     /locus_tag="PLU43_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28217.1"
                     /translation="DRYDLILMDYMMPVIDGRMATQIIRKLNGCSVEELPIIALTADV
                     LRGTKDMLLSSGMNDYLAKPIEIEKLDEILRKYIPEEKKYYKQDTGRAEDAPRLFQKE
                     DAIDEEAAMRQCMNDFGMYRLVVQTFAEESAEMQEKLEDAYAKESAEDYTIYAHALKS
                     AAASVGAMDVREIAYRMEMYGKKGHWSEITRDHEEFMKMIRELRELIGKRL"
     gene            777..>2153
                     /gene="alaS"
                     /locus_tag="PLU43_07115"
     CDS             777..>2153
                     /gene="alaS"
                     /locus_tag="PLU43_07115"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO28218.1"
                     /translation="MQKYGVNELRKMFLDFFESKGHLVLKSFSLVPHNDNSLLLINSG
                     MAPLKPYFTGQEIPPRTRVTTCQKCIRTGDIENIGKTARHGTFFEMLGNFSFGDYFKR
                     EAITWTWEFLTQVVGLDPDRLYPSVYLEDDEAFEIWNKEIGIPADRIFKFGKEDNFWE
                     HGSGPCGPCSEVYYDRGEKYGCKKPDCTVGCDCDRYIEIWNNVFTQFDNDGNGNYTEL
                     EHKNIDTGMGLERLAAAVQDVDSIFDVDTIYALRSKVCKLAGKEYHVEYKWDVSIRIV
                     TDHIRSASFMISDGIMPSNEGRGYVLRRIIRRASRHGRLLGINGMFLAKLSETVIEGS
                     RDGYPELEEKKDFIFNVLTQEETKFNKTIDQGLGILNELCENMEKDKSSVLSGEDAFK
                     LHDTYGFPLDLTKEILEEKGYAVDDAGFAKAMQEQKAQARKARKVTNYMGADATVYEQ
                     IDAAVTSKF"
BASE COUNT          647 a          364 c          587 g          555 t
ORIGIN      
        1 gaccggtatg atctgatcct gatggattac atgatgccgg taatcgacgg gcggatggcc
       61 acacagatca tcagaaaact gaatggatgt tctgttgagg agctgccgat tatcgcactg
      121 actgcggatg tgcttcgtgg aacaaaggat atgctacttt catcgggaat gaatgattat
      181 cttgcaaaac cgatcgaaat agaaaagctg gatgaaatac ttagaaaata tattccggag
      241 gaaaaaaagt attataaaca ggatacaggc agggctgagg atgctcctcg tctgtttcag
      301 aaagaagacg caattgacga agaagctgcc atgcggcagt gcatgaatga tttcggtatg
      361 taccgtctgg tcgttcagac ttttgcggag gaaagcgctg agatgcagga aaagctggaa
      421 gatgcatatg caaaagagag cgcagaggat tatacgatct atgcgcatgc gttaaaaagt
      481 gccgcggctt cagttggtgc gatggatgta cgggagatcg catacaggat ggagatgtac
      541 ggaaagaaag ggcattggag cgaaatcacg cgtgatcatg aagagttcat gaaaatgatc
      601 agggaacttc gtgaactgat cggaaaaaga ttatagcagg gataaacgtg ttaattcatt
      661 gacaattacc gtattcatgg tataaaattg tacgcgggta tttttaaatg tgactgtctg
      721 atatggacgg ttcgataaat aacagtctgc agggacggac aatcaggagg aatacagtgc
      781 aaaagtacgg tgtaaacgag cttagaaaaa tgtttttgga tttttttgaa agtaaaggac
      841 atctggtgct caaaagtttt tctttagtac cgcataatga caacagtctg ctgttgatca
      901 attccggtat ggcaccttta aaaccatatt ttacaggtca ggagatcccg ccgaggacac
      961 gggtgacgac ctgtcagaag tgtatccgca cgggagatat tgaaaatatc gggaagaccg
     1021 caagacacgg tacctttttt gaaatgctcg gtaatttttc ctttggagat tattttaaac
     1081 gggaagcgat cacttggaca tgggagtttt tgacacaggt agtaggactt gatcctgaca
     1141 gattataccc gtcagtttat ctggaggatg acgaagcatt cgagatctgg aataaagaaa
     1201 tcggcattcc tgccgacagg atctttaaat ttggcaagga agataatttt tgggagcatg
     1261 gttccggtcc gtgcggtccc tgttcggaag tttattacga ccgcggagag aagtatggct
     1321 gtaagaaacc ggattgtacg gtaggctgtg attgtgaccg ctatattgaa atctggaaca
     1381 atgtgtttac tcagtttgat aatgacggaa atggaaatta tacggagctt gagcataaga
     1441 atatcgatac cggtatgggg cttgaacgtc ttgctgccgc tgttcaggat gtggattcga
     1501 tctttgatgt tgatacgatc tatgcactgc gcagcaaagt gtgtaaactt gcagggaagg
     1561 aatatcatgt ggaatataaa tgggatgtgt ccattcgtat tgtgacggat cacatccgtt
     1621 ccgcttcttt tatgatttcc gatggtatta tgccgtccaa tgaaggaaga gggtatgtac
     1681 ttcgcaggat catccggcgc gcttccaggc atggaaggct tcttggcatt aacgggatgt
     1741 ttctggcaaa gctcagtgaa actgttattg agggctccag agatggttat ccggagcttg
     1801 aagagaagaa agattttatt ttcaatgttc tgacacagga agaaacaaaa tttaataaaa
     1861 cgatcgatca gggactggga atattgaacg agctgtgcga gaatatggaa aaagataaaa
     1921 gcagtgttct gtcgggagag gatgccttta agctgcatga tacctatgga ttcccgctcg
     1981 atctgacaaa agaaattctt gaggaaaaag gatatgccgt agatgacgca ggatttgcaa
     2041 aggcgatgca ggagcagaaa gcgcaggccc gcaaagcccg caaggtgaca aattatatgg
     2101 gtgcggatgc aacggtatat gagcagatcg atgctgcggt aacatccaaa ttt
//
LOCUS       DAOSYE010000378         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41381_length_2153_cov_2.859390, whole genome shotgun sequence.
ACCESSION   DAOSYE010000378 DAOSYE010000000
VERSION     DAOSYE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41381_length_2153_cov_2.859390"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..664)
                     /locus_tag="PLU43_07120"
     CDS             complement(350..664)
                     /locus_tag="PLU43_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28219.1"
                     /translation="MFCHICGAEIKEVIVCDIVLVDGKYHYEEVVSPILFSDEFNMRT
                     GKIFDYDFFKKAIAKKYLCTPEDVQSKYIDEIEIPAAVCVQLDKKEKDLGCRCPVCED
                     FM"
     gene            complement(694..1071)
                     /locus_tag="PLU43_07125"
     CDS             complement(694..1071)
                     /locus_tag="PLU43_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOO28220.1"
                     /translation="MELKFYKCELCGKIITIIKDTGVPTICCGQPMKEIAAGSTDAAV
                     EKHVPVVSIDGNKIAVTVGEVEHPMAEEHYIEWITISTKNGMQSKSLNPGEQPKAEFL
                     LSAGDEFVTAYAYCNLHGLWKNK"
     gene            complement(1108..1266)
                     /locus_tag="PLU43_07130"
     CDS             complement(1108..1266)
                     /locus_tag="PLU43_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOO28221.1"
                     /translation="MDKYVCVCGYVYDPALGDPDNGVEPGTAFEDIPDDWVCPMCGLG
                     KDMFEKEI"
     gene            complement(1383..>2153)
                     /gene="trpA"
                     /locus_tag="PLU43_07135"
     CDS             complement(1383..>2153)
                     /gene="trpA"
                     /locus_tag="PLU43_07135"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO28222.1"
                     /translation="AEAFASKKAFIPFITCGYPDLDTTKKVVMEMEKNGADLIELGIP
                     FSDPTAEGPVIQAANAAALKNGITTDQVFDFVAEIRKDLHIPLVFMTYANVVFSYGMD
                     RFMGKCAETGMDGLILPDVPFEEKGEFKEAAGRFGVDLISLIAPTSENRIAMIAKEAA
                     GFVYIVSSLGVTGVRSEISTDIGAIVRLIRENTAVPCAVGFGISTPEQAARMADLSDG
                     VIVGSAIIKIIAKYGKDCVPYVGSYVKSMKTAVIKSDE"
BASE COUNT          567 a          562 c          357 g          667 t
ORIGIN      
        1 ttttctctgc cggctgctgc ccactcatca atatgtgcag ccatcggcga agacggtgta
       61 atcggataaa ttgccgtcac ctcggaataa gcataagaaa cgtgagccgc tgcctcgttt
      121 ccgtccattg ttttcatttg tcttttcata ctaccatatc ctccttaaaa ttatgatcag
      181 catttttcgt aaccactttg ggcgatcact tttacaataa atcctccttt tgcatatgat
      241 acggttcaaa gaccgcatca gtataaaaaa ggaggattat tctttataaa taaatatcat
      301 cggaaccatt cttttccata ccatatcatt gatctgactg ttctgcctgc tacataaaat
      361 cttcacaaac aggacaacgg catccaagat ccttttcctt tttatccagc tgcacacaga
      421 ctgctgccgg aatttcgatc tcgtcgatat atttagactg tacatcctcc ggcgtgcaga
      481 gatacttttt ggcaattgct ttcttaaaga aatcataatc aaagatcttt cccgttctca
      541 tgttaaactc atcgctgaac aagatcggcg aaacaacctc ttcataatga tattttccgt
      601 ctaccagtac aatatcacaa acaatgacct cttttatttc tgctccgcat atatgacaaa
      661 acatttgaca agctcctctc tttacgtccg cgtttattta tttttccata aaccatgcag
      721 gttacaatat gcataagcag tgacaaattc atcaccggcg gataaaagga attctgcctt
      781 cggctgttct cccgggttta acgatttgct ttgcatacca ttctttgtgc tgatggtgat
      841 ccattcgata taatgttcct ctgccattgg atgctctact tctccaacag taacagcaat
      901 cttatttcca tctatgctga ctacgggcac atgcttttcg actgccgcat ccgtacttcc
      961 cgcagcaatc tccttcatag gctgaccgca gcatatggtg ggaacaccgg tatctttgat
     1021 gattgtaatg atctttccgc acaactcaca cttataaaac tttaattcca ttgtattcct
     1081 ctcctttctg ttattgcttc ctttggccta gatttccttc tcaaacatat ccttccccag
     1141 tccgcacata gggcaaaccc aatcgtcagg aatatcttca aatgctgttc ccggctctac
     1201 cccattatca gggtctccta atgccggatc atagacataa ccgcatacac acacatactt
     1261 atccatttgg tcacctccta tcttaattta gtcaatattt ccagttgact tcctgcagat
     1321 agaattatat cacatacctt ttatattttc cattcatttt atacattatt catataatta
     1381 atttattcat cactttttat aacagcggtt ttcatggatt ttacataact gcccacataa
     1441 ggaacacaat ctttcccata tttcgctatt atttttatga ttgcggagcc tacaataacc
     1501 ccgtcgctta agtctgccat gcgggctgcc tgttccgggg tcgaaatgcc gaatccaaca
     1561 gcacacggca cggctgtgtt ttccctgatc agccttacaa tggcaccgat atccgtggat
     1621 atctcagaac gaacgcccgt aacgccaaga gaagaaacaa tataaacaaa cccggctgct
     1681 tctttagcga tcattgcgat cctgttttca gaagtcggcg cgatcagcga gatcagatcg
     1741 actccgaacc ggccggctgc ttccttaaat tctccctttt cctcaaacgg aacatccggc
     1801 agaatcaggc cgtccattcc tgtttccgca cattttccca taaagcggtc catgccgtag
     1861 gagaacacca cattggcata tgtcataaat acaagcggaa tatgaagatc ttttcttatc
     1921 tcagccacaa agtcaaaaac ctggtcggtt gtgatcccat ttttcaaggc cgccgcattc
     1981 gctgcctgaa tgaccggtcc ttccgctgtc ggatcggaaa acggaatgcc aagttcaatc
     2041 aaatcggctc cgtttttttc catttccatc acaacctttt ttgttgtgtc caaatccgga
     2101 tagccacatg tgataaacgg aataaatgcc ttctttgatg caaaggcttc tgc
//
LOCUS       DAOSYE010000379         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41414_length_2152_cov_2.877444, whole genome shotgun sequence.
ACCESSION   DAOSYE010000379 DAOSYE010000000
VERSION     DAOSYE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41414_length_2152_cov_2.877444"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLU43_07140"
     CDS             <1..1275
                     /locus_tag="PLU43_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO28223.1"
                     /translation="LISNRYANSFYHDALSTDDVKDQLEAIDTFTSAYIWIINKNGDL
                     LYNSREQTDSSNPVTIDHFNPADLGNSYYMLGTFYEQFGEPVLSVFSPITSNFKVQGY
                     VVIHAPLRSIENTRDRLLNISYITLGIIFILSTTILIVFTDMVYIPLKKITKATEEYA
                     EGNLKYTFEINKDDEIGYLAASLNYMASELSQSEENQKKFIANVSHDFRSPLTSIKGY
                     LEAMVDGTIPPEMHEKYLKIVLNETERLTKLTNSLLALNNLNTKGMLLDITDFDINKV
                     IKNTTATFEGTCKAKHIRFKLVLTGKTMFVTADIGKIQQVLYNLIDNAIKFSDNNSYI
                     KVETTEKNETVFVSVKDSGIGIPKESLKLIFDRFYKTDLSRGKDKKGTGLGLAITKEI
                     ISAHHENINVISTVGVGTEFIFTLPKSKRLEN"
     gene            complement(1272..1799)
                     /locus_tag="PLU43_07145"
     CDS             complement(1272..1799)
                     /locus_tag="PLU43_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOO28224.1"
                     /translation="MDIREGAAECRIHNMRKPDKRGLLKAVFYIYLIFVISVVAVKYT
                     GNNLDEILKRWDIISSAKEAGERNINIVPFATISNYMKQFSRSYAYTNLIGNFVVFIP
                     FGFLLPAAFPKKRNFFAVIGLCFLFVVGIELFQYLTCLGFCDIDDLILNLLSCTVGYI
                     LYVIIMHGKARKDGN"
     gene            complement(1819..>2152)
                     /locus_tag="PLU43_07150"
     CDS             complement(1819..>2152)
                     /locus_tag="PLU43_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOO28225.1"
                     /translation="TRYINFEEFLEKNHDPVIYMATTKAKKKYTDAVFEEDAYIMFGK
                     ESAGIPEEILLQYEDTCIRIPMLAQIRSLNLANSVAIVLYEALRQQGFASMQLEGELH
                     RLHWNAEG"
BASE COUNT          671 a          508 c          406 g          567 t
ORIGIN      
        1 ctgatctcca accgttacgc aaacagcttt tatcatgatg cgctctccac tgatgatgtt
       61 aaggaccagc tggaggcgat cgatacgttt acatccgctt atatctggat catcaacaaa
      121 aacggcgatc tgctctacaa ttccagagag cagacagaca gcagcaatcc tgtcacgatc
      181 gatcatttta acccggcaga tctcggcaac agctattata tgctcggtac attttacgaa
      241 cagttcggcg agccggtact tagcgtattc tctccgatca cctccaactt taaagtacag
      301 ggctatgttg tcattcatgc accgctgcgt tcgatcgaaa acacccgtga cagactctta
      361 aatatctctt atattacact tggaatcatt tttatccttt ccactaccat tttgatcgta
      421 ttcacggata tggtctatat tccgctgaaa aaaattacaa aggctacgga agaatatgcc
      481 gaaggcaatc tgaagtatac atttgagatc aataaagacg atgagatcgg ttatctggcc
      541 gcctctttga actacatggc aagcgaactg agccagtctg aagaaaatca gaaaaaattc
      601 attgcaaatg tctcccatga cttccgctct cccctgactt ctatcaaagg atatctggaa
      661 gcaatggtgg acggcacgat cccgccggaa atgcatgaaa aatatttaaa gatcgtatta
      721 aatgagaccg aacggctgac aaagctgaca aacagtctgc tggcgctgaa taacctgaac
      781 acaaaaggca tgctgcttga catcacggat tttgacatca acaaagtgat caaaaacacg
      841 accgccacat ttgaaggaac ctgcaaggca aagcatatcc ggttcaagct ggtgctgacc
      901 ggaaaaacaa tgtttgtcac tgccgatatc ggaaagatcc agcaggtcct ttacaacctg
      961 atcgacaacg cgatcaaatt cagtgataac aattcctata taaaagtcga aacaacggaa
     1021 aaaaatgaga ctgtatttgt ctctgtcaag gattccggaa tcggaattcc gaaagaaagc
     1081 ctgaagctga ttttcgatcg tttttataaa acagacttgt cacgcggaaa agataaaaag
     1141 ggaaccggtc tcggacttgc gatcacaaag gagatcatca gcgcacatca tgaaaacatc
     1201 aatgtcatca gtacggtagg cgttggtacg gaatttattt tcaccctgcc aaaatcaaaa
     1261 agactggaaa actaatttcc gtcttttcgc gcttttccat gcataatgat tacataaaga
     1321 atatatccga ccgtacagct taacagattc aggatcagat catcaatatc gcagaaacca
     1381 agacacgtca gatactgaaa taattcgatt ccgacaacga acagaaagca gagcccgatt
     1441 accgcaaaaa aattcctttt tttcgggaac gcagccggga gcagaaatcc aaacggaata
     1501 aagaccacaa agtttccgat aaggttcgta tatgcataag aacgcgaaaa ctgcttcatg
     1561 taatttgata tcgtcgcaaa cggcacaatg ttgatatttc gctcccctgc ttcctttgca
     1621 gaagaaataa tatcccatcg tttcaaaatt tcatcaagat tatttcccgt atatttaacg
     1681 gcaacaacac tgatcacaaa aatcagatat atataaaata ccgcttttaa taatccgcgt
     1741 ttatcaggct ttctcatgtt gtgtatcctg cattctgcag ctccctctct gatgtccata
     1801 taaacccctt cccattcatt atccctctgc gttccagtgc agtctgtgca gctccccttc
     1861 aagctgcatg gaagcaaatc cctgctgccg cagtgcttca taaagcacga ttgccaccga
     1921 attcgccagg ttcagtgaac ggatctgagc aagcattggt atccggatgc aggtgtcttc
     1981 gtactgtaaa aggatctcct ccggtatgcc ggcactttcc ttaccaaaca taatatacgc
     2041 gtcttcttca aacactgcat ccgtatattt ttttttcgcc tttgtcgtag ccatataaat
     2101 aacagggtca tgattttttt caagaaactc ttcaaaattg atatatctgg tt
//
LOCUS       DAOSYE010000380         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41566_length_2147_cov_4.266730, whole genome shotgun sequence.
ACCESSION   DAOSYE010000380 DAOSYE010000000
VERSION     DAOSYE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41566_length_2147_cov_4.266730"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>2147)
                     /locus_tag="PLU43_07155"
     CDS             complement(9..>2147)
                     /locus_tag="PLU43_07155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOO28226.1"
                     /translation="ISESQERMAVVVSKEDVAQFLAYAKEENLEAVEVAVVTDSPRLV
                     LLWRGQEIVNLSRAFLDTNGAHQETGVFVEMPDENKKFFAKPELPAVETALAGGDVRA
                     AWLAVLRDLNVCSQKGLVEMFDGSIGAGSVYMPYGGRYQLTEIQTMVAKLPVLEGKTD
                     TVTMMSYGFDPYLSSWSPYHGAVYAVLESMAKVVAAGGDYSKIRFTFQEYFRRMSEEP
                     SRWSQPFAALLGAFAAQMEFGLPSIGGKDSMSGSFNEIDVPPTLVSFAVDVAKQQDII
                     TPEFKKEGSKIIVMDIKKDAYDLPCYESVMDAYGKFHQDIQNGNIISAYALDGKGLIA
                     AVSKMAFGNGLGMKIEHHIDPKKLFEAGFGSLVAEVPADRVGKLAITYTVIGEVTKAP
                     AFTYQNITLPLEEALVSWKGTLEKVFPTKAEAGMEPIETKLYEADSIYVCKNKVAEPV
                     VFIPVFPGTNCEYDSAKAFERAGAQVVTKVFTNMTAQDIRDSVEVFEKSIRQAQIIMF
                     PGGFSAGDEPDGSAKFFATAFQNAAIKEAVFDLLYKRDGLALGICNGFQALIKLGLVP
                     DGKITGQTENSPTLTFNTINRHVSKMIYTKVVSNKSPWLSQAGLGNVYVIPASHGEGR
                     FVADKEHLDKLFANGQIATQYVDFDGHISMDEEWNINGSYMAVEGITSPDGRCLGKMG
                     HSERRGEAVAVNIYGEQDMKIFESGVEYFR"
BASE COUNT          541 a          616 c          406 g          584 t
ORIGIN      
        1 caaagaagtt atctgaaata ttcgactcct gactcaaaaa ttttcatgtc ctgttccccg
       61 tatatattga cagccacggc ctccccgcgc ctttcggaat gtcccatctt tccaaggcat
      121 ctgccgtccg gacttgtaat tccctcaaca gccatatagg aaccattgat attccattcc
      181 tcatccatgg aaatatgacc gtcaaaatca acatactgcg tagcgatctg accatttgca
      241 aacaatttat ccaaatgttc tttatccgct acaaatctgc cttcgccgtg agatgccgga
      301 atcacataga catttccaag tccggcctgt gaaagccatg gcgatttatt tgaaacgacc
      361 tttgtataga tcatttttga aacatgccgg ttgatcgtat taaaggtaag tgtcggtgaa
      421 ttttccgtct gtccggtaat tttaccgtca ggtacaagcc cgagcttgat cagcgcctga
      481 aatccgttgc agataccgag cgccagcccg tcccttttat aaagcagatc aaaaaccgct
      541 tctttgatcg cagcattctg gaatgccgtt gcaaaaaact ttgcggaacc gtccggttcg
      601 tcacccgcgg aaaacccgcc gggaaacata atgatctgag cctgtctgat actcttctca
      661 aacacttcca cagaatccct gatatcctgc gccgtcatat ttgtaaatac cttcgtaaca
      721 acctgtgccc cggcacgttc aaatgctttt gcactgtcat attcacagtt cgttcccgga
      781 aagaccggaa tgaatactac cggctctgcc actttatttt tacatacgta aatactgtcc
      841 gcttcataaa gctttgtttc aatcggctcc atacctgcct ctgctttagt cggaaatact
      901 ttttcaagcg ttcctttcca tgaaacaagt gcctcttcca gcgggagcgt aatattctga
      961 taagtaaatg ccggtgcttt cgtcacttcg ccgattaccg tataggtgat cgccaatttc
     1021 ccgaccctgt cggccggcac ctccgctaca agcgaaccaa aaccggcttc gaataatttc
     1081 tttggatcta tatgatgctc tattttcata cccagtccat tgccgaacgc catcttgctg
     1141 acagccgcga tcagcccttt tccatcaagc gcataggctg aaataatatt gccgttctga
     1201 atatcctgat gaaactttcc gtacgcatcc ataacagact catagcatgg cagatcatag
     1261 gcatcctttt ttatatccat aacgatgatc ttactgcctt cttttttaaa ctccggtgta
     1321 ataatatcct gctgctttgc cacatcgacc gcaaatgaca caagtgtcgg cggcacatcg
     1381 atttcattga acgaaccgga catgctgtcc ttcccgccga tagacggcag gccgaattcc
     1441 atctgcgcgg caaacgcgcc gagcagcgcg gcaaacggct ggctccatct ggatggttcc
     1501 tcactcatcc gtctgaaata ttcctgaaat gtaaatcgta ttttcgaata gtctccgcct
     1561 gcagccacta cctttgccat ggactcaagc accgcatata cggccccatg ataagggctc
     1621 cacgaggaaa gatacggatc aaacccataa ctcatcatcg ttaccgtgtc cgtttttcct
     1681 tcaagcaccg gcagctttgc gaccatcgtc tgtatttccg tcagctgata tctgccgccg
     1741 taaggcatat agaccgagcc tgctccgatc gagccgtcaa acatctccac aagtcccttt
     1801 tgagaacaga cattcagatc acgcagcacc gcaagccacg ccgctctgac atctcccccg
     1861 gcaagtgccg tctccaccgc aggaagctcc ggctttgcaa aaaacttttt attttcatcc
     1921 ggcatttcca caaaaacacc ggtctcctga tgtgctccgt ttgtatcaag aaatgctctt
     1981 gaaagattga cgatctcctg tcctctccat agaaggacca gacgcggcga atctgtaaca
     2041 actgccactt cgacagcctc cagattttct tcttttgcat atgccagaaa ctgcgcaaca
     2101 tcctcttttg aaacaacaac tgccattctt tcctgtgatt ccgatat
//
LOCUS       DAOSYE010000381         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41604_length_2146_cov_3.817312, whole genome shotgun sequence.
ACCESSION   DAOSYE010000381 DAOSYE010000000
VERSION     DAOSYE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41604_length_2146_cov_3.817312"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..519
                     /locus_tag="PLU43_07160"
     CDS             46..519
                     /locus_tag="PLU43_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO28227.1"
                     /translation="MNEIAFTLNGEKTSYSGNAADRLLDVLRETYGLTSVKCGCKEGE
                     CGACSVLIDGKLMNSCCVAMGAVAGSDVVTMEGYRETERFKVLDRAFGEMSAVQCGFC
                     IPGMVLASEALLAANPHPTDAQIRDGLSGNLCRCTGYNAIVRAVLAAAKEGDGLW"
     gene            513..1331
                     /locus_tag="PLU43_07165"
     CDS             513..1331
                     /locus_tag="PLU43_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOO28228.1"
                     /translation="MVNGYLADSLEEALKIRSEQTVIPYAGGTDLMIANRQEAPYLFI
                     GKLEELKQITVDENYVRIGGAVTFTQALGSDLIPPLMKEALSRIAAPAIRNAGTFAGN
                     IGNGSAKADSVLIEYAADAKICLRSLRGERIVDIDQFYLGRKKLDLKEDELITEILLP
                     KAGLENYYYKKVGGRNALAISRVAFTGIIRINNGTIESFAAAFGAVAGTVLRFKDLEK
                     QLVGKTLDEAKEMKKDILDAYAEKMVLTKGRVSAEYRKTVCMNLLGDFMEEKGI"
     misc_binding    1412..1633
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1685..>2146
                     /locus_tag="PLU43_07170"
     CDS             1685..>2146
                     /locus_tag="PLU43_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOO28229.1"
                     /translation="MGMIGHIKNEIRVIRERDPAIHCAMEVFLYPSFKVMLHYRIAHK
                     LYLKRHYFLARYISQRAVRKTGIEIHPGAEIGSGFFIDHGNGVIIGETSIVGNNVTLY
                     QGVTLGGTGKECGKRHPTIEDNVMISAGAKVLGSFTIGANSKIGAGSVVLEE"
BASE COUNT          614 a          385 c          627 g          520 t
ORIGIN      
        1 aacagaggat gttgtgaagg cagtaacgga aagagaggga aaacgatgaa cgaaatagca
       61 tttactttaa acggcgaaaa aaccagttac agcgggaatg ctgcggatcg tctattggac
      121 gtgcttcgtg aaacctatgg tctgacatcg gtcaaatgcg gatgcaagga aggagagtgc
      181 ggagcctgtt ccgtgctcat cgacggaaaa ctgatgaatt cctgctgtgt tgcaatggga
      241 gcagttgcgg gcagtgatgt agtgacaatg gaaggctatc gggaaaccga gcgatttaaa
      301 gtacttgaca gggcgtttgg tgaaatgtcc gcagtacagt gcgggttctg tattccgggc
      361 atggttttgg catcggaagc gctgctggct gcgaatccgc atccgacaga tgcgcagatc
      421 cgtgacggac tctccggaaa tttatgccgc tgtaccggtt acaatgcgat cgtaagagcg
      481 gttttggcag cagcaaagga gggtgatgga ttatggtaaa cggatatctg gcagattctc
      541 ttgaggaggc tttgaaaatt cgttctgagc agacagtgat cccgtatgcg ggaggaacgg
      601 atctgatgat tgcaaaccgt caggaggcac cgtatttatt tataggaaag ctggaagaat
      661 taaaacagat cacagtggat gaaaactatg tcagaatagg cggagccgta acgtttacac
      721 aggcacttgg ctcagatctg attccgccgc tgatgaaaga ggcattatcc agaattgctg
      781 cccctgcgat ccggaatgcc ggtacatttg cagggaatat cgggaatggt tcggcaaaag
      841 cggactcggt tctgatcgaa tatgcggcag acgcaaagat ctgcctgcgt tcattgcggg
      901 gtgagcgtat cgtggatatc gatcagtttt atcttggcag aaaaaagctg gatctgaaag
      961 aggatgagct gattactgaa atactgctgc caaaggcagg acttgaaaat tattattata
     1021 aaaaagtcgg cggcagaaat gcactggcaa tttcccgtgt tgcatttacg ggtatcatac
     1081 ggataaacaa cggaacgatc gaatcgtttg cagcagcgtt cggagcggtt gcaggcactg
     1141 ttctgcggtt taaggatctg gaaaagcagc ttgtcggaaa gacgcttgac gaagcaaagg
     1201 aaatgaaaaa agatattttg gatgcgtatg cggaaaaaat ggttcttaca aaaggacgtg
     1261 tttccgcaga gtacagaaaa acagtctgca tgaatctgct cggagatttt atggaagaaa
     1321 agggaatctg aagtgaaatt ccatttgaca aaccttttgt ttttgcatag actgtattat
     1381 ataggaacgt atcgttgatc ggtgcgtaac agagaatgcg gagaacggga agagtaggtc
     1441 ttgaaagata acagagaggg agtgtcatcg gctggaagca gtcccggttc ggaaaagacc
     1501 aaagtgcatc cgggagcaga tcgggcgaat atacagtagt ccggtacgta tggcatacgt
     1561 tacatgcatc gagggaaaga cgattgttct tttaacagag tgggaccgcg gagtttacag
     1621 cttcgtctct tttttggaga cgaagcttat tttgtgggtt ataataatga aggagctgtc
     1681 atttatgggg atgattgggc atattaaaaa tgagatccgt gtaatacggg aacgggatcc
     1741 ggctatacac tgtgcaatgg aagtattttt atatccgagt tttaaagtta tgctgcatta
     1801 caggatcgcg cataagctgt atctgaaaag gcattacttt ctggctcgtt atatttccca
     1861 gcgtgcggtc agaaagaccg gaatcgagat ccatcctgga gcagagatcg gaagcggatt
     1921 ttttatcgac catgggaacg gtgttatcat cggagagaca tcgatcgttg gaaacaatgt
     1981 gacgctgtat cagggagtga cgctcggagg aacaggaaaa gagtgcggaa agcgccatcc
     2041 gacgatagaa gataatgtca tgatcagtgc cggtgccaag gtgcttggtt cgtttacgat
     2101 cggggcaaat tccaaaatcg gcgcaggttc ggtcgtgctc gaagaa
//
LOCUS       DAOSYE010000382         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41618_length_2146_cov_2.790053, whole genome shotgun sequence.
ACCESSION   DAOSYE010000382 DAOSYE010000000
VERSION     DAOSYE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41618_length_2146_cov_2.790053"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLU43_07175"
     CDS             complement(<1..324)
                     /locus_tag="PLU43_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HOO28230.1"
                     /translation="MYKAFLRIMKKNYLFSFGCIICSFWIIMALFADFIAPFSPTEQN
                     LAIKLNAPSLTHIFGTDNFGRDIFSRVIYGGRYSLLAGCVTVVLVAVIGSAYGAIAGY
                     VGGMID"
     gene            complement(354..1373)
                     /locus_tag="PLU43_07180"
     CDS             complement(354..1373)
                     /locus_tag="PLU43_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28231.1"
                     /translation="MERLKFICKRLLFLIVMLIGVATIVFILTKIVPGDPVVANLSQR
                     NLNNPEIVAAFKAKYGLDKPVIVQYFLYMKNIFQFNLGTSIRTNKPVIAELSRCFPAT
                     AELALFAIIMASLFGILFGILSAIKRNSIIDQIVRAISVVGVSIPSFWFALIVLFIFY
                     YKLKIMPGPGRLSSIFDAPAAVTGFYTIDSLLEGNVSKFLDALKHLILPGGVLAAFTM
                     GLITRTTRSNLLDVMSTDYIRTARAKGLSKKRIIWRHALGNALIPVLTVIGLGFGNLL
                     GGMVLVETIFNWPGVGQFAYNSVKALDYPSIIGVSLLIALNYMIINTIVDILYGVIDP
                     RVRIK"
     gene            complement(1455..>2146)
                     /locus_tag="PLU43_07185"
     CDS             complement(1455..>2146)
                     /locus_tag="PLU43_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28232.1"
                     /translation="DPDGQQAIRYAVDYAGIQNVCGEGSVTPKSIIQVGFMGSAGELD
                     TATAQDLDKAKELMEKAGYADGFDIDMPVCELDMEGVPLLDLAQKVKEDLSAININVN
                     IVQQNWSGGYGDDYRAGNIGLTVMYWGIDYNDPNVQLAFLPGEEVGLRAGWTADGHEE
                     TLDLYDQILSATDNEVRSDLLEQLQSSIESDCPFIMLAQAACHIGYSSSLDGVAFSDT
                     YRIDVTSVNVK"
BASE COUNT          648 a          533 c          373 g          592 t
ORIGIN      
        1 gtcgatcatt ccgcctacat aacctgcgat cgcaccgtaa gcagagccga tcacagcaac
       61 aagaacaact gttacacagc ctgcaagaag cgaatacctg cccccataga taactctgct
      121 gaaaatatca cgtccaaaat tatcagtccc aaaaatatgt gtcagagacg gcgcatttaa
      181 tttgatggca agattctgtt ctgtcggact gaaaggcgcg ataaaatcag caaacaatgc
      241 cattataatc caaaatgaac aaataataca gccaaaagaa aataaataat tctttttcat
      301 aattcttaaa aatgctttat acatacaata actgcctcct gtcatctgcc tttctatttt
      361 attctgacgc gcggatcgat cactccatac aggatatcaa cgatcgtgtt gatgatcatg
      421 taattcagtg cgatcaataa agacactcct ataatagacg gataatcaag tgcttttacc
      481 gaattatatg cgaactgacc gactcccggc cagttaaaga tcgtttcgac caataccatg
      541 ccgccaagaa gattcccaaa gccaagtcca atgaccgtaa gaacaggaat caaagcattt
      601 ccaagtgcat gacgccatat gatccgcttt ttggaaagtc ccttggctct tgccgtacgt
      661 atgtaatctg ttgacattac atccagcaga tttgacctcg tcgttcttgt gatcagaccc
      721 attgtaaaag ctgccagcac gccgcccggc aaaatcaaat gtttcaatgc atccagaaac
      781 ttggatacat taccttccag cagactatct attgtataga atcccgtgac cgctgccgga
      841 gcatcgaaga tcgaactcag cctgcccggt ccgggcataa ttttcaattt ataataaaaa
      901 ataaaaagaa cgatcagggc aaaccagaaa cttggtatac tgacaccaac tacggaaatt
      961 gcccgaacga tctggtcaat gatactgttt ctcttgattg cggataaaat accaaacaaa
     1021 ataccaaaca aagatgccat aataattgca aacaatgcta attcggcagt tgccggaaag
     1081 cacctggaca gctcggcaat caccggttta ttcgttctga tcgatgtacc gagattaaac
     1141 tgaaaaatat tcttcatata caggaaatat tgtacaatga ccggtttatc caatccatat
     1201 ttcgctttaa atgccgcaac gatctcaggg ttgtttaaat ttctctgact taagtttgct
     1261 acaaccgggt ctccgggaac tattttcgtc aatataaaga cgatcgtagc tacgccgatc
     1321 agcatgacga tcagaaacag cagcctttta caaatgaatt ttaaccgttc catgcgattc
     1381 ctctttctga atttttatac agcagtaaat gcagcttcct gcacttacca ctgtatattt
     1441 aacaacacaa tctattattt tacatttaca cttgtcacat caattctata cgtatctgag
     1501 aatgcgactc cgtcaaggct gctgctgtaa ccgatatggc aggctgcctg tgccagcata
     1561 ataaacggac aatcactttc aatactgctc tggagctgtt caagcagatc ggaacgtacc
     1621 tcattatctg ttgccgataa gatctgatca tacaagtcta aggtttcctc atgaccgtcg
     1681 gctgtccatc cggctcttaa tcctacttct tcaccaggca ggaacgcaag ctgtacattc
     1741 gggtcattat aatcgattcc ccagtacatc acagtaagcc caatatttcc ggcacgataa
     1801 tcatcaccat atccgcccga ccagttctgc tgaacaatat ttacattgat attgattgct
     1861 gaaagatctt cttttacttt ttgtgcaagg tccagaagcg gtacgccttc catatccagc
     1921 tcacataccg gcatatcaat atcaaatccg tctgcatacc ctgctttttc catcaattct
     1981 tttgctttat ccagatcctg cgctgttgct gtatcaagtt ctcctgcact gcccataaat
     2041 ccaacctgaa taattgattt tggcgtaaca gagccttctc cgcacacgtt ttgtattcct
     2101 gcataatcaa cagcataacg gatcgcctgc tgtccatccg gatctg
//
LOCUS       DAOSYE010000383         2144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41685_length_2144_cov_2.174246, whole genome shotgun sequence.
ACCESSION   DAOSYE010000383 DAOSYE010000000
VERSION     DAOSYE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41685_length_2144_cov_2.174246"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLU43_07190"
     CDS             <1..1045
                     /locus_tag="PLU43_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28233.1"
                     /translation="MTSRVLVMYAGNILEEGPTQELLMEPFHPYTRGLIYSSPAVNPY
                     RDMWGIPGEIENSGEGACPFYKRCSQRIEQCACSHPVLETVCRKRSVSCLRGGIVTLL
                     HGNGLKKEYRTGKRMIKACRGCDIEIKAGEVCALIGESGSGKTTLAEILSGILRPDDG
                     KIVFDGKCIDGNSATSKIGGIQIVFQDPLSATNERLSIEEIVREPLDILHNGTKDERT
                     ASVKEALKNVQLAYDAAFLKKRGFMLSGGQRQRVAVARALVMRPKLLIADEISAMLDP
                     STAANLLRLLKGLQNTEGFAMLYITHDLALAQKISDQVYVMHEGVIIEQGPAKDVLRN
                     PKEAYTKLLLGRT"
     gene            complement(1059..1289)
                     /locus_tag="PLU43_07195"
     CDS             complement(1059..1289)
                     /locus_tag="PLU43_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOO28234.1"
                     /translation="MTQIMQIHTKENYRLEVTLDNGSSILLNLKPKLDTLRFGLLRDE
                     AFFRCAKTDGHVIHWDGKVELSISEIFELIKK"
     gene            complement(1401..>2144)
                     /locus_tag="PLU43_07200"
     CDS             complement(1401..>2144)
                     /locus_tag="PLU43_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOO28235.1"
                     /translation="RKDMTDQVEYQFLRTVELCEISFYTWIGCKYKIPESLVKPETGR
                     IRLMFTAHAMYNIIYGRVLLISESYTELLGSADYFFETAAVYPNLLGVVYTHIFLAAA
                     NQKLFRENKALDHLKKAMHIAMPDDLFMPFVENCDYIAPLLHALSVQELFREQIEQIL
                     ALYRLYEKSKKSILQCWLSDSKPLLTEREMNVSKLVAEGLTNHEIAERLFISVNTVKA
                     VLKAIYAKLAINNRVRLKQYVDELDNTAL"
BASE COUNT          580 a          452 c          533 g          579 t
ORIGIN      
        1 catgacgagc cgtgtacttg ttatgtatgc gggcaatatt ctggaagaag gaccgacaca
       61 ggaactttta atggagccgt ttcatcctta tacaagggga cttatttatt cttcgcctgc
      121 agtcaatcct tatcgcgata tgtggggaat tccgggagag atcgaaaatt ccggcgaagg
      181 agcatgccct ttttataaac gctgcagtca gcggatcgag cagtgtgcct gttcgcatcc
      241 ggttttggaa acggtctgca gaaaaagaag tgtatcctgt ctgcggggcg ggatcgtgac
      301 actgctccat gggaacggtc tgaaaaaaga atacaggaca ggaaaaagga tgatcaaagc
      361 atgccgtgga tgcgatattg aaataaaggc gggcgaagta tgtgcgctga tcggagaatc
      421 cggttccgga aaaacgactc tggcggagat tctgtccggc attttacgtc cggatgacgg
      481 aaagatcgta tttgacggaa aatgtattga cggaaacagt gctacgtcaa agatcggcgg
      541 gatccagatc gtctttcagg atccgttgtc cgcaacgaat gaacgtctgt cgatcgaaga
      601 gatcgtacgg gaaccgcttg acattctcca caatggaaca aaagacgagc ggactgcctc
      661 tgtaaaagaa gcgctgaaaa atgtacagct tgcatatgat gcagcctttt taaaaaagcg
      721 cggatttatg ctcagcggcg gtcagcgaca gagggtagcg gtggcgagag cacttgtcat
      781 gcgtccgaag cttctgattg cagacgagat cagcgccatg ctggatcctt cgacggcagc
      841 gaatcttctg cggcttctga aaggacttca gaatacggaa ggctttgcaa tgctttacat
      901 tacccatgat ctggcgcttg cacaaaagat atccgatcag gtatatgtaa tgcatgaagg
      961 cgtaatcatc gaacagggac cggcaaagga tgtcctgcgt aatccgaagg aagcatatac
     1021 gaagctgctt cttggcagga cttaagatac aggcagattt attttttgat cagttcaaaa
     1081 atttcgctga tggacagttc tactttgccg tcccagtgga tcacatggcc gtctgttttt
     1141 gcgcaccgga aaaatgcttc atcgcgcagc aatccgaaac ggagcgtatc aagctttggc
     1201 ttcagattaa gcagaatgct gctgccgtta tcaagagtga cctcaagccg gtaattttct
     1261 tttgtatgga tctgcatgat ctgtgtcaaa gggttcctcc cattataaaa taaacataaa
     1321 gcagccacaa tacaaacagt gtatcgtgac tgcttttaaa tttgtattac ccgaatgggt
     1381 atattttgaa tatttatata ttagagtgcc gtattgtcca gttcatcgac atattgtttt
     1441 aaacggactc tgttattgat ggcaagcttg gcgtagatcg ctttcagtac ggctttgacc
     1501 gtgttaacag atataaaaag ccgttctgcg atctcatggt ttgtcagtcc ttcggcgact
     1561 aatttggaaa cattcatctc tctttcagtt aacaggggtt tgctgtcgga aagccagcat
     1621 tgcaggatgc tttttttgga tttttcatat aatcggtaca acgccagaat ttgctcgatc
     1681 tgttctctga acagttcctg aacggaaagc gcgtgcagga gcggtgcgat ataatcgcag
     1741 ttttccacaa acggcatgaa gagatcatcg ggcatggcaa tgtgcattgc ttttttcaga
     1801 tgatcaagcg ctttgttttc acgaaaaagc ttttgattcg cagccgcaag aaaaatatgt
     1861 gtatagacga cgccaagcag atttgggtaa accgctgcgg tttcgaaaaa ataatccgca
     1921 ctgcctaaaa gttccgtata actttcgctg atcagcagga cccttccgta tatgatatta
     1981 tacattgcat gggccgtgaa catcagacgg atacgtccgg tttccggctt cacaaggctt
     2041 tccggtattt tgtatttgca gccgatccac gtataaaagc ttatttcaca caattcgaca
     2101 gtacgcagga attgatattc cacttggtcc gtcatatctt tgcg
//
LOCUS       DAOSYE010000384         2140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41800_length_2140_cov_2.489209, whole genome shotgun sequence.
ACCESSION   DAOSYE010000384 DAOSYE010000000
VERSION     DAOSYE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41800_length_2140_cov_2.489209"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLU43_07205"
     CDS             complement(<1..277)
                     /locus_tag="PLU43_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO28236.1"
                     /translation="MIYTLTYSPSIDYLVYLDELEQGKLNLMKKDAVFYGGKGVNVSQ
                     VLQGLGVESTALGFVAGFTGREIEDGLERRGCKTDFVHLKEGHSRINI"
     gene            complement(369..1301)
                     /locus_tag="PLU43_07210"
     CDS             complement(369..1301)
                     /locus_tag="PLU43_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28237.1"
                     /translation="MRHIKRLFFCFLLFGLLTGCRETEAVSDETVDLPRIICWGDSLT
                     EGTGGDGVTYPNTLEELSGREVINYGVYAETASCIAARQGGNPMHTRASLTIPSACEP
                     VELILDNEKGNPEMLLVFGDAGINPCSIGGVEGTLSIDMETGNRYFTRNEAGEPVTIE
                     AGTQMLFHAMLDKRTDDILVIFSGTNDYPDTESIEDVIAYQRAMLAYAGAENYVIIGM
                     TKKKAMPKIEEINQILEEEYGEHFLDIRKYLLENGLQDAGVTETQQDRADLESGEIPS
                     SLRTDDEGHGTSEFYRIIGEQLYLKLLELNYLTR"
     gene            complement(1330..>2140)
                     /locus_tag="PLU43_07215"
     CDS             complement(1330..>2140)
                     /locus_tag="PLU43_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28238.1"
                     /translation="IICWGDSLTESVDGRTAYPDVLAQLTETEVVNYGVRSETTRTIA
                     MREGAVPVYTGEFVIPEDTSLTPLQITNSTQFLKNGTAGVNPCYIGGVAGTLSYDGTA
                     GTYYFSRLSAGESVYVEAGSRITTNGMADKQKEDVLIIFTGTNDAPDPSSIYDIITIQ
                     KAMLAYTGCENYLIIGLTCKKVMPKIEELNEILAEEYGEHFLDIRSYLLTYGLEEAGI
                     EASAQDLADLEDGEIPSSLRRDYVHGNEVFYQILAGQIYRKLQYLGYLPLE"
BASE COUNT          544 a          610 c          377 g          609 t
ORIGIN      
        1 taatattgat cctggaatgc ccctctttta aatgtacaaa atccgttttg cagccgcgcc
       61 tttccaatcc gtcttcaatt tcacggcctg taaaaccagc cacaaatcca agcgccgtac
      121 tctctacacc gagtccctgc agcacctggg aaacattgac ccccttgccg ccgtaaaaaa
      181 cagcgtcttt tttcatcagg ttcagtttcc cctgctctaa ttcatcaaga taaaccaaat
      241 agtcgatcga cggactatag gtaagcgtat aaatcataaa tctcctccaa acacaaatgt
      301 cgtatggata tcataccatt tttcccgccg caaaacaagc ttccatatta tgcaaaatca
      361 taacccctct accgggtcag atagtttaac tccagcagct ttaaatagag ctgttcccct
      421 atgatcctgt aaaactcact tgtaccatgt ccttcgtcgt ctgttctaag gctcgatggg
      481 atctctccgg attccaggtc agcccggtcc tgctgtgtct cagtaacgcc ggcatcctgc
      541 agcccgtttt ccaataaata ttttctgata tccagaaaat gctccccgta ctcttcttca
      601 agtatctgat tgatctcctc gatctttggc attgcctttt tctttgtcat tccgatgata
      661 acataatttt cagctccggc atacgcaagc attgcccgct gatatgcaat gacatcctct
      721 atgctctccg tatccggata atcattcgta ccggaaaaaa taacaagaat atcatctgtt
      781 cttttatcga gcatcgcatg gaacagcatc tgcgtgccgg cctctatcgt aaccggttcc
      841 cccgcttcat ttcttgtaaa atatctgttt ccagtttcca tatcgatcga aagtgtacct
      901 tccacgccgc cgatgctgca tggattaata ccggcatctc cgaacacaag cagcatctcc
      961 ggatttccct tctcattatc caatatgagc tctaccggtt cacatgcgga cggaatcgta
     1021 agcgaggctc ttgtatgcat cggattaccg ccctggcgcg cagcgataca gcttgctgtt
     1081 tccgcataga ccccataatt gataacctcc ctgccgctta actcttcaag tgtattcgga
     1141 taagtcacac catcgccgcc ggtaccttcc gtcagggagt ctccccagca gatgatccgc
     1201 ggcagatcaa ctgtttcgtc actaaccgct tccgtttccc tgcagcctgt cagcaaacca
     1261 aatagcaaaa aacaaaaaaa tagtcttttt atatgtctca tttattctct cctaactgtt
     1321 attcctatcc tattctaatg gaagataacc taaatactgc aatttcctgt aaatctgccc
     1381 tgccagtatc tgataaaata cctcattccc atgcacataa tccctgcgca gacttgatgg
     1441 aatttcgccg tcttcaagat ctgccaaatc ctgcgcggat gcctcgatcc ctgcctcttc
     1501 aagtccataa gtgagcagat agcttcggat gtccaaaaaa tgttcaccat attcttctgc
     1561 caggatctca ttcagctcct ctattttggg cattaccttc ttgcaggtaa gtccaataat
     1621 cagatagttc tcacatcctg tatacgctaa cattgccttc tgtattgtaa tgatatcata
     1681 tatggaagac ggatccggcg catcatttgt tcccgtaaaa ataatcaaaa catcttcttt
     1741 ctgtttatct gccatcccat ttgtcgtaat ccggctgcct gcctccacat aaacggactc
     1801 tcctgcagaa agacgggaaa aataataagt cccggccgta ccgtcatagc tgagtgtacc
     1861 ggcaacgcct ccgatatagc agggattgac tcccgccgtt ccgtttttga gaaactgcgt
     1921 gctgttcgtt atctgaagcg gcgtcaaaga cgtgtcctcc ggaataacaa actctcccgt
     1981 ataaaccgga accgctcctt ctctcatggc aattgtcctt gttgtctcgg aacgaactcc
     2041 ataattgact acttcagtct ccgtcagctg cgccagcaca tcaggataag ccgtccggcc
     2101 atcaacactt tccgttaaag aatctcccca gcagataatt
//
LOCUS       DAOSYE010000385         2137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_41883_length_2137_cov_3.179155, whole genome shotgun sequence.
ACCESSION   DAOSYE010000385 DAOSYE010000000
VERSION     DAOSYE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41883_length_2137_cov_3.179155"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>2137
                     /gene="ileS"
                     /locus_tag="PLU43_07220"
     CDS             71..>2137
                     /gene="ileS"
                     /locus_tag="PLU43_07220"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOO28239.1"
                     /translation="MYKKVSTDLGFVEREKEVEKFWKENHIFEKSMENRKEGETYTFY
                     DGPPTANGKPHIGHVLTRVIKDMIPRYRTMKGYMVPRKAGWDTHGLPVELEVEKALGL
                     DGKEQIEEYGLDPFISKCKESVWKYKGMWEDFSATVGFWADMDNPYVTYHDDFIESEW
                     WALKQIWEKDLLYKGFKIVPYCPRCGTPLSSHEVAQGYKDVKERSAIARFKVKDEDTY
                     ILAWTTTPWTLPSNVALCVNPVEMYAKVKAADGFTYYMAQALLDTVLGPLANKETGIP
                     AYEILETYTGKDLEFKEYEPLFDYAVDICQKQKKKAFYVTCDSYVTLTDGTGVVHIAP
                     AFGEDDANVGRNYDLPFVQLVDGKGQMTKETPWAGTFCKDADKLVLVELEKNGLLFSA
                     PKFEHSYPHCWRCDTPLIYYARESWFIKMTAVKEDLIRNNNTINWIPESIGKGRFGDW
                     LENIQDWSISRNRYWGTPLNVWECECGHMHAVGSREELKELSGREDAMTAELHRPYID
                     EFTITCEKCGKQMHRVPEVVDCWFDSGAMPFAQHHYPFENKELFESQFPAQFISEAVD
                     QTRGWFYSLLAESTLLFNKAPYENVIVLGHVQDENGQKMSKSKGNAVDPFDALDTYGA
                     DAIRWYFYINSAPWLPNRFHGKAVMEGQRKFMGTLWNTYAFFVLYANIDQFDATKYTL
                     EYEKLPV"
BASE COUNT          629 a          391 c          581 g          536 t
ORIGIN      
        1 ttctcaaaag ttatccacag ataacgaaaa tgaattatga aatttcttca attatgaaag
       61 gacgactgtt atgtacaaaa aagtttctac agatttaggt tttgtggaaa gagaaaaaga
      121 agtagagaaa ttttggaaag agaatcatat ttttgaaaaa agtatggaaa acaggaaaga
      181 aggagaaacc tatacgtttt atgacgggcc tccgactgca aatggaaagc cgcatatcgg
      241 tcatgtgctg acgcgtgtta ttaaagacat gattccgaga taccgtacga tgaagggata
      301 tatggttcca aggaaggcag gctgggatac gcacggactg ccggtagagc tggaagtgga
      361 aaaagcgctc ggtctggacg gaaaagagca gatagaagaa tacggcctgg atccgtttat
      421 ttcaaagtgc aaggaaagtg tctggaaata taaaggaatg tgggaggatt tttccgcgac
      481 tgttggtttt tgggcggata tggacaatcc ttatgtgact tatcacgacg attttattga
      541 atcggagtgg tgggccctaa agcagatctg ggaaaaggat ctgttgtata aagggttcaa
      601 gatcgtcccg tactgcccgc gctgcggaac accgctttct tcccatgagg tggcgcaggg
      661 ctataaggat gtaaaggaac gttctgcaat tgcgcggttt aaagttaaag acgaggatac
      721 ctatattctg gcatggacaa cgacgccgtg gacgcttcct tccaatgtgg cgctttgtgt
      781 gaaccctgtt gagatgtatg caaaagtaaa agccgcagat ggctttactt attatatggc
      841 acaggcgctt ttggatacgg tgcttggtcc gctggccaat aaagaaacgg gaataccggc
      901 atatgagatt ctggagactt ataccggaaa agatctggaa ttcaaggaat atgagccgct
      961 gttcgattac gcggtagaca tttgccaaaa gcagaaaaag aaagcgtttt atgtgacatg
     1021 cgattcatat gttacgttaa cagatggtac cggtgtcgta catatcgcgc cggcctttgg
     1081 tgaagacgat gcgaacgtag gacgcaatta tgacctgccg tttgtacagc ttgtagacgg
     1141 caaggggcag atgacaaagg aaacgccgtg ggcaggcact ttctgcaaag atgccgacaa
     1201 gcttgttctt gtggagctgg aaaaaaatgg gctgctgttt tctgcaccga aattcgagca
     1261 cagctatccg cattgctggc gctgtgatac gccgctgatc tattatgcaa gagaatcctg
     1321 gtttataaaa atgacggccg taaaagagga cctgatcaga aataacaata cgatcaactg
     1381 gattccggaa tcgatcggaa agggccgttt cggtgactgg ctggagaata ttcaggactg
     1441 gagcatttcg agaaatcgtt actggggtac cccgctgaat gtatgggaat gtgagtgcgg
     1501 gcatatgcat gctgtcggaa gccgtgagga attaaaggaa ttgtccggca gggaagatgc
     1561 catgacggca gagctgcaca ggccgtatat cgacgagttt acgatcacct gtgaaaaatg
     1621 cggaaaacag atgcacagag tgccggaagt cgttgactgc tggtttgatt cgggtgcaat
     1681 gccgtttgcg cagcatcatt atccgtttga aaacaaggaa ctgtttgaaa gtcagttccc
     1741 tgcacagttt atttcagaag ctgtagacca gacaagaggc tggttctatt cgctgctggc
     1801 tgaatcaacg ctgctgttta ataaagcacc gtacgaaaat gtcatcgtac tcggccatgt
     1861 acaggatgaa aacgggcaga agatgagcaa atccaaagga aacgcagttg atccgtttga
     1921 cgcgcttgat acatatggag ctgatgcgat cagatggtat ttttatatta attctgcgcc
     1981 atggcttcca aatcgttttc acgggaaagc tgttatggaa ggacagcgca agtttatggg
     2041 aacactgtgg aatacttatg cgttttttgt attatatgcc aatattgacc agtttgatgc
     2101 aacaaagtat acattggaat atgaaaagct gccggtc
//
LOCUS       DAOSYE010000386         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42337_length_2123_cov_2.777563, whole genome shotgun sequence.
ACCESSION   DAOSYE010000386 DAOSYE010000000
VERSION     DAOSYE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42337_length_2123_cov_2.777563"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLU43_07225"
     CDS             <1..1035
                     /locus_tag="PLU43_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HOO28240.1"
                     /translation="RYDAREFVFPMSATKQLTEDELMNESDDSLSFARNEIFARHGKQ
                     FDDYYLQCYFDSCSWYTGTSADIGAEALNETEAYNVELIKKAEEDYQASHPYPKKVLC
                     GDSVNETLCTELGMQVIDYRVSGDADTGSYDGIITIDGQDFKLSDFGIDPEVPDEEEF
                     FITDINPYLDGLEIAILDFGNNDFNTTYFFGYAADQKLYYIGEVPGCPFKGVASASCN
                     GFADEGAVIGTVRTDLLETASVYVSYWYDYDGKALVEQDAGCRKMVPSEAHELYMDLP
                     VYNSMDENSDTVLLKAQKEVYFMKNDNREWIAVKGKDGVEGYIHVNDGVITNIGEDAA
                     NVFSGLGFSG"
     gene            1104..2042
                     /locus_tag="PLU43_07230"
     CDS             1104..2042
                     /locus_tag="PLU43_07230"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28241.1"
                     /translation="MDFYTVSQWLLFFYIYCFIGWVWESGYVSVKEKRLVNRGFMHGP
                     FLPIYGSGAIVILFATLPVKGNIVLVYVFGMLSATVLEYCTGACMEGLFKVRYWDYSE
                     LPLNLNGHICFFVSLGWGVFSIVLVRFAHLPIEAFVLSIPKTASDITAMLLTIYVTVD
                     FTQSFNEAMDLREMLAKLSESNEQLRRLEKRIEVISAVVGDDLQKRAENKAVKIQSKK
                     ERIEVILNRTREEKTRRLAGLEEQANLYFAKRKPGSDEEKSYMAELNKLRQRLHMQTD
                     KEYYSLRRILERNPGAVSRLQSDALKEIKEMFHMKK"
BASE COUNT          605 a          360 c          590 g          568 t
ORIGIN      
        1 aggtatgatg cgcgggaatt tgtttttccg atgagtgcaa cgaagcagct tactgaagat
       61 gaactgatga atgagtcgga tgacagtctt tcctttgcac gcaatgaaat atttgcaaga
      121 catggaaagc agtttgatga ctactattta cagtgctatt ttgattcctg ttcctggtat
      181 acgggaacgt ctgctgatat cggagccgaa gcactgaatg aaacggaagc atataatgtg
      241 gaactgatca aaaaggcgga agaagactat caggcttctc atccgtatcc gaaaaaagtg
      301 ctgtgcggcg attctgtaaa tgagacgctt tgcacggagc ttggcatgca ggtgatcgat
      361 tacagggtgt caggggacgc cgataccggt tcatatgacg ggatcattac gatcgacgga
      421 caggatttta agctgagtga ttttggaatc gatccggaag taccggatga ggaagagttt
      481 tttataaccg atatcaatcc atatttggac gggctggaga ttgcgatact ggattttggg
      541 aacaatgatt ttaacactac gtattttttt ggttatgcgg ccgatcaaaa gctgtattat
      601 atcggtgagg tgccgggctg tccgtttaag ggcgtcgctt ccgcgtcctg caacggattt
      661 gcggacgaag gcgctgtgat cggaacagta cgtaccgatc tgcttgaaac ggcaagtgtc
      721 tatgtgagct actggtatga ttatgatgga aaagcgctgg tagaacagga tgcgggatgc
      781 agaaaaatgg ttccgtcaga ggcgcatgaa ctgtatatgg acctgccggt ctataattcg
      841 atggatgaaa attccgatac ggtactattg aaagcccaga aggaagttta ttttatgaaa
      901 aatgacaaca gggaatggat cgctgtcaaa ggaaaagacg gtgtagaggg atatattcat
      961 gtgaatgacg gggtcataac aaatatcgga gaggatgcgg caaacgtgtt ttccgggttg
     1021 gggttttccg gctgaatttt cctggactgc atttgattta ggacagaaga tttttgaata
     1081 cagaaaaaca ggagagtgtc gggatggatt tttatacggt atcacagtgg ctgctgttct
     1141 tttatattta ttgctttatc ggctgggtat gggagtccgg ctatgtgtcg gtaaaagaga
     1201 aaagattggt aaacagggga tttatgcatg gtccgttttt gccgatctat ggttcgggtg
     1261 caattgtgat tttatttgct acccttccgg tcaagggaaa tatcgtgctt gtatatgtgt
     1321 tcgggatgct gagtgccact gttttggaat attgtacggg tgcctgcatg gaaggattat
     1381 tcaaagttcg ttactgggat tacagtgaac tcccgctcaa tttgaacggg catatctgtt
     1441 tttttgtatc gcttggatgg ggagtgtttt ccattgttct tgtaaggttt gcgcatcttc
     1501 cgatcgaagc gtttgttctt tcgattccaa agacagcatc ggacattacg gcaatgctgc
     1561 tgacgatcta tgtgacagtc gactttacgc agtcgttcaa cgaggcaatg gatctgcgcg
     1621 aaatgcttgc aaaattgagc gaaagcaacg agcagctgcg cagacttgaa aaacgtattg
     1681 aagtcatatc tgcagtagtg ggggatgatc ttcaaaagcg tgcggaaaat aaggcggtga
     1741 agatacagtc caaaaaagag aggatcgaag tgatcctgaa ccggacacgc gaagagaaaa
     1801 cgcgccgtct ggcaggactt gaggaacagg caaaccttta ttttgcaaag cgtaaaccgg
     1861 gaagtgatga agaaaagagc tatatggctg aactgaataa actgcgccag aggctgcata
     1921 tgcagacgga taaggaatat tacagcttaa gacgaatact ggagagaaat ccgggagcgg
     1981 tctccagact gcagagtgat gcgctgaagg aaatcaagga aatgtttcat atgaaaaagt
     2041 gaaattgaaa aacgtacttg cgtaaaaccg caggatagtg atactattaa gaagttctat
     2101 acaacagcat atacggaata ggt
//
LOCUS       DAOSYE010000387         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42428_length_2120_cov_3.064891, whole genome shotgun sequence.
ACCESSION   DAOSYE010000387 DAOSYE010000000
VERSION     DAOSYE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42428_length_2120_cov_3.064891"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PLU43_07235"
     CDS             <1..1277
                     /locus_tag="PLU43_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28242.1"
                     /translation="TRLAFNSSYFCLCALYSFHDFGQSMHTLSGLIAVIVMLYAAAGF
                     FMNLNKKEKLLTACNFVRLSQFLYFVIICQELISPTTDFVTIYFIIWLSIRWLALLEK
                     KEKEIAPYAILCVVAVFLVSIKLSVGILALLVIWPAYRLIREKRIKDILCYVLLGILL
                     ILPYFIRNVLISGWLIYPFAGLDLFSVDWKVPASDVRYEADEITVWARYTKDAKLIGQ
                     SIREWFPVWWQEQGRENRYFSLAAFLALGLEVLQFIRAAIRFIRTKSTAGLEGLYYGA
                     IFSAGFLFWLFSAPANRFGYAYLLVLPLLVMGSMLAQRDRFLIRIMKFAASAVIIGAL
                     GFGIYLLVPADIAYVQNNLNGDYLIAQKDYPTVETGSRNFEGLTICFPAEEGAQIWYH
                     AFPAILYEGNLDSIERRGDTVRDGFRLKIQRE"
     gene            1325..1846
                     /locus_tag="PLU43_07240"
     CDS             1325..1846
                     /locus_tag="PLU43_07240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO28243.1"
                     /translation="MEHPVITITMENNDVMKAELYPETAPNTVNNFISLVKKGFYDGL
                     IFHRVINGFMIQGGCPEGSGMGGPGYSIKGEFNQNGIKNDLAHTEGVLSMARAMDPDS
                     AGSQFFIMHKTSAHLDGAYAAFGKITEGLDIVDKIAQTDTDYSDRPRTPQKIKSMTVE
                     TFGVDYQEPEKTA"
BASE COUNT          585 a          392 c          535 g          608 t
ORIGIN      
        1 agacaaggct tgcgttcaac agctcgtatt tctgtctttg cgccctgtac agctttcatg
       61 attttgggca gtccatgcac acattatcgg ggctgatcgc ggtaattgtc atgctttatg
      121 cggcagcagg cttttttatg aatcttaata aaaaagaaaa gctgcttacg gcatgtaatt
      181 ttgtgcggct gtcgcaattt ttgtattttg tgatcatatg tcaggaactg atctctccca
      241 cgacagattt tgtaacgatc tattttatta tctggctttc gatcaggtgg ctcgcactgc
      301 ttgaaaagaa agaaaaagag atcgcgccgt atgccatttt gtgtgtagtc gcggtatttc
      361 ttgtttcaat aaagctttct gtcggcattc tggcgctgct tgtgatctgg ccggcatatc
      421 gtctgatcag ggagaagcgg atcaaagata ttctgtgcta tgtattgctt ggcattcttt
      481 tgatcttgcc gtattttatt agaaacgtgt tgatttcggg ctggttgatc tacccgtttg
      541 caggactcga tctgttttcg gtggactgga aggttccggc ctccgatgta cgctatgaag
      601 cggatgagat cacagtatgg gcgcgttaca cgaaagatgc gaagctgatc ggacaaagta
      661 tcagggagtg gtttccggtc tggtggcagg aacagggacg ggaaaatcgt tatttttccc
      721 ttgcggcttt tttggcgctc ggattggaag tgctgcagtt tatcagagcc gcaatccgct
      781 ttataaggac aaaaagcacg gccggtctgg aggggcttta ttacggagcc atattttccg
      841 cgggtttttt attctggctt ttttcagccc cggcgaaccg ttttggatat gcatacctgc
      901 ttgtgctgcc gcttcttgtg atgggaagca tgcttgcgca gcgtgatcgg tttttgatca
      961 gaattatgaa atttgcggca tctgccgtaa taataggagc gcttggattt ggtatatatc
     1021 tgcttgtgcc tgcagacatt gcgtatgttc aaaataattt gaacggcgat tacctgattg
     1081 ctcagaagga ttatccgacg gtcgagaccg gaagccggaa ttttgaggga cttacgatct
     1141 gttttccggc agaagaaggc gcgcagatct ggtatcatgc gtttccggca attttatatg
     1201 aaggaaatct ggacagcatc gaaagaagag gagatacggt gcgtgatggg ttccggctga
     1261 aaatacaaag ggaatgatcc ctgaatttta ataataaaaa ttataaaagg aaaaggtgaa
     1321 aataatggaa catccagtta ttacgattac aatggaaaat aatgacgtta tgaaggcgga
     1381 gctttatccg gaaacggcgc cgaatacggt taataatttt atcagtcttg tgaaaaaagg
     1441 attttatgac ggcctgatct ttcacagagt gatcaatggt tttatgatcc agggaggctg
     1501 tccggaagga agcggtatgg gaggaccggg gtatagcatc aaaggagaat ttaaccagaa
     1561 tggtatcaag aatgatcttg cccatacgga aggggttctt tccatggccc gtgccatgga
     1621 tccggactcg gccggctccc agttttttat tatgcacaaa acgtctgcgc acctggacgg
     1681 cgcgtatgcg gcatttggaa agattacgga aggactggat attgtagata aaattgcgca
     1741 gacagatacg gattattccg acaggccacg tacgccgcag aagattaaat ccatgactgt
     1801 ggaaactttt ggagttgatt accaggaacc ggaaaaaaca gcataattac aaagaaatac
     1861 ggaattggaa aaaataaatt atgaaatatg acgaaaagcc ggaggatgta ggaaaataga
     1921 gggtttggcg ttcatgaaat gttcatattt catttaaaaa agtttcactc acacaacatt
     1981 gtttggacat ttatgtgaac tatactaaga ccataagata aagcaagaca ataaaaacgc
     2041 caaactcata gtaacattac tattaaaata ctttttcata ataaatgcca agtgaccagg
     2101 tcacttggca tcctcccttt
//
LOCUS       DAOSYE010000388         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42533_length_2117_cov_3.392338, whole genome shotgun sequence.
ACCESSION   DAOSYE010000388 DAOSYE010000000
VERSION     DAOSYE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42533_length_2117_cov_3.392338"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLU43_07245"
     CDS             <1..225
                     /locus_tag="PLU43_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HOO28244.1"
                     /translation="PAVQVVSEELLFRLIRASFNQRRKTLANSLCNSPELAFTKEQVL
                     QVLKQMGVSENIRGEALTLQQFAKLSNLLS"
     gene            728..>2117
                     /locus_tag="PLU43_07250"
     CDS             728..>2117
                     /locus_tag="PLU43_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28245.1"
                     /translation="MDKYEYQVCADQIKSLLAERNYTEAVDIADTIDWRRVKSVSMLC
                     TVSEIYKINKRYEESRDILLLAYERYPNGRMIVYALCELAIKMDDVLQAIEYYKEFAQ
                     IAPEDTGSYILLYKIYEAQEVSLEERIEVLEEFKKQDYREKWAYELACLYHKTGQETK
                     CVEECDELILWFGEGKYVKKAMELKMEHTKLTSEQQMKYEGRQEETQPEQIAFVQPEE
                     QSYMDSVNQQPFMQMPESPYGMEQQQYGQPYMEHPYVNNSEQPAQIPYPAVDDIQVQP
                     VNTGKYSTLNLQEEIARNVGEVLANGENPIQAPYYGQMESLYEPAQEPENYQSMYGQP
                     SAEAVQQMYEPLPAEAEQQMYEQPPVEAEQQIYEQPAGAKIIPYVQPAVQAAQEPQAE
                     KKFDSILAQEYDGQISLSLPDTDMVEKQITGQISLDDILKGWEEKRKESEKKRLAEVK
                     RRSLEQTNDIMSQ"
BASE COUNT          747 a          351 c          508 g          511 t
ORIGIN      
        1 cctgccgtac aggtcgtgag tgaagaattg ttattcaggc ttattcgggc atctttcaat
       61 cagaggagaa aaacactggc caacagcctc tgcaattcgc cggaactggc atttacaaag
      121 gaacaggtgc tgcaagtatt gaagcaaatg ggtgtttccg aaaatatccg cggggaagca
      181 ctgacactgc agcagtttgc aaagctgagc aatttgctgt cataaaaagc acatatagta
      241 cgaattatta aaacaaaacg aaataataca gaaaaataaa ctattaatta attattaaat
      301 atataaataa actgttaatt cattgacaaa aagcaggctc catgttataa tcattttatc
      361 aatcagaaac ctgcttctta tttttaacaa aagtgaagat aaagttatca aaataatgtt
      421 tgatgatata tttgctataa gaaggcaggc ggttgattat ataaatatga cgtggaaaaa
      481 aggaggagag tccaatggac acagcagggt tttagcagcc gttttggcag atgtcaaaac
      541 ggctgttttt atgtgtttca taggtgcttt ttgaccgaat atttttgaca atgcactagt
      601 cctatggtaa acttagttag aaatgtatag atattgttgt tttgcttata aaaaaggata
      661 ccattttaat aaaagaatac atattttatt tatagaatca ttgataaaag catgaggtga
      721 aagttaattg gacaaatatg aataccaggt ttgtgccgat cagatcaagt cattgcttgc
      781 cgaaagaaac tatacggaag cagtggatat tgcagacacg atcgactggc gcagggtaaa
      841 aagtgtttcg atgctttgta cggtcagtga gatatataaa attaataagc gatacgaaga
      901 aagccgggat atattactcc tggcttatga gcgttatcca aacggaagaa tgatcgttta
      961 tgccttatgt gagcttgcca ttaaaatgga tgatgtgctg caggcaatcg aatattataa
     1021 ggaatttgca cagatagcgc ccgaggatac cggcagctat attttactgt acaaaattta
     1081 tgaagcacag gaagtttctc ttgaagaaag aatagaagtg ctggaagaat ttaaaaaaca
     1141 ggattacaga gaaaaatggg catatgaatt ggcatgtctt tatcataaaa caggacagga
     1201 aacaaagtgt gtagaggaat gtgatgaact gattctgtgg tttggggaag gcaaatatgt
     1261 aaaaaaagcc atggaattga aaatggaaca tacaaaactg acgtcagagc agcagatgaa
     1321 atacgaagga cgacaggaag aaacacagcc tgaacagata gcttttgtac agccggagga
     1381 gcagtcttat atggactctg taaatcagca gccctttatg cagatgccgg agagtcccta
     1441 tggaatggag cagcagcaat acggccagcc ctatatggaa catccgtatg tgaataattc
     1501 ggaacagccg gcgcagatac cgtatcctgc ggtagacgat attcaggtac agcccgttaa
     1561 tacaggtaag tatagtacgc ttaatctgca ggaagagatc gcaaggaacg taggagaggt
     1621 tctggcaaat ggagagaacc ctattcaggc accttattat ggtcagatgg aaagcctgta
     1681 tgaaccggca caggaaccgg agaactatca gtcaatgtat ggacagcctt cggcggaagc
     1741 agtacagcag atgtatgagc cgctgccggc agaagcagag cagcagatgt atgaacagcc
     1801 gccggtagaa gcggagcagc agatatatga acagccggca ggagcaaaaa tcataccgta
     1861 tgtacagcct gcagtacagg cagcacagga accacaggca gaaaagaagt ttgattccat
     1921 acttgcacag gaatacgacg ggcagatctc tttgagcctg ccggataccg acatggtgga
     1981 aaaacagatt acagggcaga ttagtctgga tgatatttta aaaggctggg aagaaaaaag
     2041 aaaagaaagt gaaaaaaagc ggcttgcaga ggtaaaaaga agatctcttg agcagacaaa
     2101 cgatattatg tcacaac
//
LOCUS       DAOSYE010000389         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42537_length_2117_cov_3.209505, whole genome shotgun sequence.
ACCESSION   DAOSYE010000389 DAOSYE010000000
VERSION     DAOSYE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42537_length_2117_cov_3.209505"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLU43_07255"
     CDS             complement(<1..903)
                     /locus_tag="PLU43_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28246.1"
                     /translation="MGERHQKRNVGLLICVIVLCIAVVCLAVWLIKDRMHGKESFEDV
                     ILNLEFDDKEDLYWVENISSKELLRMQKNGHIHFDSDGPREFILKYRIKGGELVHYRV
                     RNAESGPSFFAGLSYGTAGSKSEMQQIRFIPNSFSPVSCIYMVDFMDGGEPLGSWEGE
                     FQGSAIMNTGQWHDVIYYLPEDADSVYVILSDEEQDKIAYLSFVLPEDWRHEDWQLML
                     NPFFDEGVSGQSVDLDFVRVSDSTLSDYLKNHISSYQTHKERLDQFLAKGADQIPVLS
                     NQEQEETTGDESQAREEEADEQNGG"
     gene            complement(1005..1697)
                     /locus_tag="PLU43_07260"
     CDS             complement(1005..1697)
                     /locus_tag="PLU43_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOO28247.1"
                     /translation="MKRSKINQCIREMELMAKTYHFALPPFCAWTPEEWKTKNHEYDE
                     IRDNMLGWDITDCGLDNYDKIGFSLITLRNGNQKNDKYRKVYAEKLIMLKEGQQFPFH
                     FHWTKSEDIINRGGGTLIIHLYNDDGNGGFSDEDVLVNSDGRSFMVPAGTGIELKPGQ
                     SITLWPHQYHNFDTVPGTGDLLIGEVSMCNDDMTDNRFHDKVGRFPKIEEDEQPYRLL
                     CNEYPAAAGCFN"
     gene            complement(1755..>2117)
                     /locus_tag="PLU43_07265"
     CDS             complement(1755..>2117)
                     /locus_tag="PLU43_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO28248.1"
                     /translation="VPEYFMNNINLAASNDVLIAISQSGVTKDILNGVDLAKKRGLKV
                     IAITAYVNSSLAEKSDIVLESKGDFSRFDYYKNYNHLCEIAVVDVLCELIQQKFAVMN
                     RKGKDDDMLELLLSEAKL"
BASE COUNT          564 a          558 c          377 g          618 t
ORIGIN      
        1 tccgccgttc tgctcatcag cttcctcttc ccgtgcctga ctttcatctc ccgtagtttc
       61 ttcctgctcc tgatttgaca gcaccggaat ctgatccgct cctttggcaa gaaattgatc
      121 cagtctttct ttatgtgtct gataagaaga aatgtgattc ttcagatagt ctgacagcgt
      181 actgtccgaa acccggacaa aatccaggtc cacggattgt ccgcttactc cttcatcaaa
      241 aaaaggattc agcatcagct gccagtcttc atgccgccag tcttccggaa gcacaaaaga
      301 aagatacgct atcttgtcct gttcttcgtc actcagaata acatagacac tgtccgcatc
      361 ttcaggcaga taatagatca cgtcatgcca ctgccccgta ttcataatgg ccgaaccctg
      421 aaactctcct tcccagctgc caagcggttc tccgccatcc ataaagtcaa ccatatagat
      481 gcacgaaaca ggagaaaaag aattcggtat aaacctgatc tgctgcattt ccgactttga
      541 tccggcagtc ccatatgaca gaccggcaaa aaaggaaggg ccgctttcag cgtttctgac
      601 tctgtaatgt acaagttctc cacccttaat cctgtatttg agaataaatt cccgtggtcc
      661 gtcactgtca aaatggatat gcccattctt ttgcatacgc agaagctctt ttgaggaaat
      721 attttcaacc cagtacagat cttctttatc atcaaattcc aaattaagta ttacatcctc
      781 aaagctttct tttccatgca tcctgtcctt gataagccat actgccaaac aaaccacagc
      841 aatgcataaa acgatcacac agataagcaa tcccacattc cttttttgat gcctttcacc
      901 cattttctct ccccctgctt ctccggagcc acccggctgc cctgcagcag tcttacgaag
      961 ccagcagaat atttctgatt tatcagacgg aacagacaat attgtcagtt aaaacagccg
     1021 gctgctgccg gatactcgtt gcataacagc cgatatggct gctcatcttc ttctatttta
     1081 ggaaaacgtc cgactttatc atgaaaacgg ttgtccgtca tatcgtcgtt gcacatggaa
     1141 acctccccaa tcagcagatc tcccgttccg ggcaccgtat caaaattgtg atactgatga
     1201 ggccagagcg taatactctg tccgggcttc agttcaattc ccgttccggc cggaaccata
     1261 aatgaccggc cgtcactatt gacaagaaca tcttcatctg aaaagccgcc gttcccgtca
     1321 tcgttatata aatgtatgat cagcgtgccg ccgccccggt tgataatgtc ttcgcttttc
     1381 gtccagtgaa aatgaaaagg aaactgctgg ccttccttca gcatgatcag cttttcggca
     1441 tatactttcc gatacttgtc atttttctga tttccattac ggagcgtaat cagtgaaaag
     1501 ccgatcttat cataattatc gagtccgcag tccgtaatgt cccagccaag catattatcc
     1561 ctgatttcat catactcatg attctttgtc ttccattcct ccggtgtcca tgcgcagaat
     1621 ggcggaagtg caaaatgata tgtttttgcc attaattcca tttcccggat acattgattt
     1681 atttttgatc ttttcatagt tttccccatt gatacaaaaa actgctcgaa aaggcttggg
     1741 cagccccctg ttttctataa tttggcttcc gatagcaaaa gctccagcat atcatcatcc
     1801 ttgcctttcc gattcataac ggcaaacttc tgctgaataa gctcacataa aacgtcgaca
     1861 accgcaattt cacacaaatg attataattc ttatagtaat caaatctcga aaagtcacct
     1921 ttcgattcaa gaacgatatc cgatttctca gcaagactgg aattgacata tgctgtaatg
     1981 gcaattactt ttagtccccg tttttttgca aggtcgacgc cgtttaaaat atcttttgtc
     2041 acgcctgact gtgaaatggc aatcagcaca tcgttggacg ctgcaagatt gatattgttc
     2101 ataaagtatt ccggcac
//
LOCUS       DAOSYE010000390         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42539_length_2117_cov_2.951988, whole genome shotgun sequence.
ACCESSION   DAOSYE010000390 DAOSYE010000000
VERSION     DAOSYE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42539_length_2117_cov_2.951988"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLU43_07270"
     CDS             <1..783
                     /locus_tag="PLU43_07270"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO28249.1"
                     /translation="ALLVMLAYTMQIYFDFSGYCDMAAGICLMLNIDLPVNFDSPYKA
                     KSITEFWKKWHITLTRFFRVYVYFPLGGNRKGKIRTYINMFLIFFLSGLWHGAAYTFI
                     IWGMLHGIGICLSKLLAKWTVKLPSFVRWLGTFLFVNITWIYFRAPDLASANAFIGQL
                     FSFQFQPLSIALVAAATPAEANLAQWLIQNSSGSAPYYSGAVIILGVLVFSVFAGTVM
                     KNTQERVQSFKASKGILAVTVLLLIWSILSLSEVSKFIYVNF"
     gene            815..1864
                     /locus_tag="PLU43_07275"
     CDS             815..1864
                     /locus_tag="PLU43_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28250.1"
                     /translation="MEYGKKFTIKTISYTLGILILLAAVTVIIDPFIHYHAPICGLAA
                     VETDERSALVGVARNMDYDTALIGSSMSENFKESWFEDGVFGNSCVKLSLQGAHFNDY
                     DVLFREVSKKKDLKNIVFSLDTYLLTNPPEDYPVTIPSYLSNTSYWDDASYVWNKSVL
                     LEYLPKFLINNVTEHYNADSAYTWADDYVFSKYAARGSYNAERLLIPEEEKSYDTYFT
                     YSDEFLADIIPYIESCPDVTFYFYSSPYSILFWDDSVRRGNTTAEICALSRVFEKLLS
                     YDNVRIFYFQDDYDIITDLDNYRDYSHFDQPINYYMYECMRDGKKEVTLDTYYDTLLN
                     MYQFAIAYDYESAFH"
     gene            1996..>2117
                     /locus_tag="PLU43_07280"
     CDS             1996..>2117
                     /locus_tag="PLU43_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28251.1"
                     /translation="MNTDKTAELISRRRKMKNLTQKELAMQLGVTDKAVSKWERG"
BASE COUNT          551 a          475 c          438 g          653 t
ORIGIN      
        1 gcccttcttg tcatgctcgc ctatacgatg cagatctatt ttgacttttc cggctactgt
       61 gatatggccg ccggtatctg tctgatgctc aatatcgatc tgccggtaaa ttttgattct
      121 ccttataagg caaaatcaat tacagaattc tggaaaaagt ggcatatcac cctgacaagg
      181 tttttccggg tctatgtcta ttttcctctt ggcggcaaca gaaaggggaa aatccgtacc
      241 tacattaata tgtttctcat ctttttctta agcggcctct ggcacggtgc cgcctatacg
      301 tttattatat ggggaatgct tcatggcatc ggcatctgtt taagcaaact gcttgcaaaa
      361 tggacggtaa agcttccttc cttcgtgcgc tggcttggta ctttcctttt cgtaaatatc
      421 acctggatct atttcagggc tcccgatctt gcttctgcca atgcctttat cggacagctg
      481 ttctcttttc agtttcagcc gctcagcatt gcgcttgtcg cagcagccac gccggcggaa
      541 gccaatctgg cacaatggct gatccaaaac agctccggct ccgcgccgta ctactccggt
      601 gctgtcatta ttctcggcgt actggtcttt tccgtatttg caggtactgt tatgaaaaac
      661 acccaggagc gtgtccagtc cttcaaggca tcaaaaggaa tactggccgt tacggtgctg
      721 ctgcttatat ggtcgatctt atctctgtcc gaagtttcaa aatttattta tgtcaatttt
      781 taatcgtaca atatttttat ataatggaaa aactatggaa tatggaaaaa aatttaccat
      841 taagacaatt tcatatacgc ttggaatttt gatcctgctg gccgccgtta ctgtcattat
      901 cgatccgttt atccactatc acgcgccgat ttgcgggctc gcggcagtgg aaaccgatga
      961 acgttccgcc ctggtcggtg tcgcaagaaa catggattac gatacggccc tgatcggttc
     1021 ttccatgagc gaaaacttta aggaatcatg gtttgaagac ggtgttttcg gaaacagctg
     1081 tgtcaagctg tccttgcagg gcgcccattt caatgattat gatgtcctgt ttcgtgaagt
     1141 ttcgaaaaaa aaagacttga aaaatattgt tttcagtctg gatacttacc tgctgacaaa
     1201 tccgcccgaa gattaccctg tcacgattcc ttcttatctg tccaacacat cttattggga
     1261 tgatgcctct tacgtatgga ataagtccgt actgctggaa tatttgccga agtttctgat
     1321 caacaacgtg acggaacact ataacgccga ttccgcatat acatgggcgg acgactatgt
     1381 cttttccaag tacgccgcac gtggttctta taatgcggaa cgtcttctga tcccggaaga
     1441 agaaaagtcc tatgatactt attttacata cagtgatgaa ttcctggcag atattatccc
     1501 ttatattgaa agctgtcccg acgtaacatt ttatttctat tcgtcaccat acagcatcct
     1561 gttctgggac gacagcgtac gccgcggcaa tacgaccgca gagatctgtg ctctttcacg
     1621 tgtatttgaa aaattgcttt cttacgacaa tgtccgtatt ttctattttc aggacgacta
     1681 tgatattatt acggacctgg acaattaccg ggattattcc cattttgacc agccgatcaa
     1741 ttactatatg tacgaatgca tgcgcgacgg caaaaaggag gtaacgcttg atacgtacta
     1801 tgatacactg ctgaatatgt atcagtttgc aattgcatat gattatgaat ccgcttttca
     1861 ctaatatccc atcagcggat gcttttgttc tctacttttg gtttacccta tgaatttcaa
     1921 ccgttagtcg gttgttatat atgagtgttt ccctttacaa ttattatata atttaaaatg
     1981 aaaggaatca taaaaatgaa taccgataaa acagcagaac ttatttcccg cagacgaaaa
     2041 atgaaaaacc ttacgcaaaa agaactggca atgcagttag gagttacgga taaggccgtt
     2101 tccaaatggg agcgggg
//
LOCUS       DAOSYE010000391         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42561_length_2116_cov_2.789908, whole genome shotgun sequence.
ACCESSION   DAOSYE010000391 DAOSYE010000000
VERSION     DAOSYE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42561_length_2116_cov_2.789908"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1553)
                     /locus_tag="PLU43_07285"
     CDS             complement(300..1553)
                     /locus_tag="PLU43_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOO28252.1"
                     /translation="MVVELISVGTELLLGNIVNTNAAYLSEKCAGLGLSCFYQSVVGD
                     NEKRLIHTIETALTRSDIVILSGGLGPTEDDLTKDAAAGVFGKEMRVDEDAREHIQEY
                     FEKKGMEIAANNWKQAMVPEESVVLINENGTAPGFIMEQKGKVMILLPGPPNEMIPMF
                     EKNIIPYLSRKTNSIIYSQVVKLCGISESAAEEKLADMVKSENPTVATYAKTGEVHIR
                     VTAKEEDEKGAKKLIKPVVKELKNRFGTAVYSTDTDTTLEQSIVELLSENHLTITTAE
                     SCTGGMVAARIVNVPGASEIFKEGFITYSNKAKRRYLGVKKRTLDKYGAVSEQTAKEM
                     AKGGILFTKSDLCIAVTGIAGPDGGTVEKPVGLVYISCAFGGKTYVEKCCFTGNRMKI
                     RENTAVEALVFLRMCVLQHYNKMNS"
     gene            complement(1611..2114)
                     /gene="pgsA"
                     /locus_tag="PLU43_07290"
     CDS             complement(1611..2114)
                     /gene="pgsA"
                     /locus_tag="PLU43_07290"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOO28253.1"
                     /translation="MIPVFLVFMLTEAGGAAGKWIALALFIAASLTDLLDGYIARKYN
                     LITNFGKFMDPLADKLLVTSAMLCFVELGSLPAWIVIIIISREFIISGFRLIASDNGV
                     VIAASYFGKFKTTFQMLMVCLLIADIPGMVFLVITKICIWIALILTVVSLIDYIVKNK
                     DVLKSHR"
BASE COUNT          553 a          536 c          365 g          662 t
ORIGIN      
        1 atcgcataga acatctgttc gtctttttaa gaataaaaca tctgttcgtt tttgtcaacc
       61 gctttccgat atttttactt tttttctaag cttctaaact ttcactttta ttgattttac
      121 catttttatt gtcctattaa aagtacaata ataaccgata ctttgccgtt aatggaatat
      181 tttactataa aatcatgaaa ttcatggcga actgcctgat atgagtttgt tctgccgccg
      241 gataacccga tcattcccat ggctatccgc aaacggcttc tgaatatttg attccgtatt
      301 tatgaattca ttttattgta atgctgaagt acacacatcc gtagaaaaac cagtgcctca
      361 acagccgtat tttccctgat cttcatcctg tttccggtaa aacagcattt ttcaacatac
      421 gttttcccgc caaacgcgca ggaaatataa acaagtccta cgggcttctc caccgtacct
      481 ccgtccggac ctgcaattcc ggtcacggca atacaaaggt ctgattttgt aaacaaaatg
      541 ccgcccttgg ccatctcctt tgctgtttgc tcactgaccg ctccatactt atcaagtgtt
      601 cttttcttta ctccgaggta gcgtcttttg gctttattgg aataggtaat aaacccttct
      661 ttaaatattt ccgacgctcc cggcacattt acgatccttg ccgccaccat gccgcccgta
      721 caggactctg ccgtagtaat cgtcaaatga ttttccgaaa gcagctccac tatggactgc
      781 tccagcgtcg tatccgtatc ggtcgaataa acagcagtac cgaaacgatt ctttaattct
      841 tttacaacag gcttgatcag ttttttagcc cctttttcat cttcttcctt tgccgttaca
      901 cggatatgta cctcgcctgt tttggcatat gtggcaaccg tcggattttc cgatttcacc
      961 atatcggcca gtttttcttc cgcggcgctt tcactgatcc cgcacagctt tacgacctgt
     1021 gaatagatga tcgaattagt ttttctgctt aaatatggaa tgatgttttt ttcaaacatc
     1081 ggtatcattt cattcggcgg tccgggaagc aggatcatta ccttcccctt ttgttccatt
     1141 ataaatccgg gcgccgttcc gttttcattg atcagtacaa ctgattcttc cggaaccatt
     1201 gcctgtttcc agttattcgc ggcaatttcc attccctttt tctcaaaata ttcctgaata
     1261 tgctctctgg cgtcttcgtc taccctcatt tcttttccga acacccctgc cgcagcatct
     1321 tttgtcagat cgtcctccgt aggaccgagg ccgccggata aaatcacgat atcagaacgg
     1381 gtaagcgctg tttctatcgt atggatcagc cgcttttcat tgtctccgac cacactctga
     1441 taaaagcagg aaagtccaag accggcacat ttttcagaca gataggcagc attcgtattt
     1501 acgatgttac caagcaataa ttctgttccg acgctgatca gttccactac cataaagcaa
     1561 ttcctctctt cttctccgtt tatatctttt tcacgtacag ccaatgaaaa ttatctgtga
     1621 ctttttaaaa catctttatt ctttacaata taatcgatca atgaaacaac cgtaaggatc
     1681 aatgcgatcc agatacagat ttttgtaata accaaaaaaa ccatgcccgg aatatccgca
     1741 atcagcagac agaccatcag catctgaaat gtcgtcttaa attttccaaa ataacttgcg
     1801 gctatgacta ccccgttatc ggatgcgatc agacggaagc cgctgataat aaattcccgg
     1861 ctgataataa taatgactat ccaggccgga aggctgccaa gttccacaaa gcagagcatt
     1921 gcagaggtaa caagaagctt atccgcaagc ggatccataa attttccgaa gttcgtaatc
     1981 aaattatatt ttcttgcgat atatccgtcc aacaggtccg tcagactcgc agcgataaaa
     2041 agtgcaagtg cgatccattt tcctgcggcc cctccggctt cggtcagcat aaacacaaga
     2101 aataccggta tcatca
//
LOCUS       DAOSYE010000392         2111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42713_length_2111_cov_3.271401, whole genome shotgun sequence.
ACCESSION   DAOSYE010000392 DAOSYE010000000
VERSION     DAOSYE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42713_length_2111_cov_3.271401"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLU43_07295"
     CDS             complement(<1..1053)
                     /locus_tag="PLU43_07295"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HOO28254.1"
                     /translation="MMAVEKVIQFGEGGFLRGFVDYFLYKLNEKGVWEGKVVVVQPIE
                     KGMCELLSAQNCAYHLYLRGIENGQMVNERTYIDVISRCINPYEEFEEYRKLAENPDI
                     RFIISNTTEAGIEYRGTEKLSDRPAGSYPAKLTQLLYRRFELGLKGFILMPCELIDKN
                     ADHLKECVIQYAKLWELPQAFLTWLDEENDFCNTLVDRIVTGYPKEEAEELWKEIGYQ
                     DHCLDTAEIFHLWVIEGNHEKELPFNKAGYHIVWTDDVQPYKRRKVRILNGGHTSMVL
                     GAYLYGLQTVEECMHDETVSAFLKKCIFDEIIPTLGNTQTDIEFGKAVIERFSNPYIK
                     HRLLSIALNSVSKFAVR"
     gene            complement(1100..>2111)
                     /locus_tag="PLU43_07300"
     CDS             complement(1100..>2111)
                     /locus_tag="PLU43_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOO28255.1"
                     /translation="GEIMLRLSTPGNERFIQADEFDVCYGGGEANVAVSLANYGYDTQ
                     YVTAVPDNPIGDCAVASLKKYNVGCDFIIKDGERLGIYYLESGSAMRPGNVIYDRTNS
                     AVATISADMFDFSAIFENADWFHFTGITPALSDSAAELTEAACRAAKAAGVMVSVDLN
                     FRKKLWSPQKAQKVMTNLMQYVDVCIGNEEDAELALGFKTEASSVVTGELDLMEYKKV
                     FHQMADKFGFRYIAGSLRRSRSASDNGWSACMIDGKTGEFYHSKTYAITPIVDRVGSG
                     DSFAAGVICGLLDEKNIKESLEFAVAASALKHTIPGDFNLVSRMEVERLVKGDGSGRV
                     RR"
BASE COUNT          558 a          603 c          372 g          578 t
ORIGIN      
        1 ccgtacggca aacttgctta cggaatttaa tgcgatcgac aacagccggt gcttaatata
       61 cggattagaa aaccgttcta tcaccgcttt gccaaactcg atatcggtct gcgtatttcc
      121 gagcgttgga atgatctcat caaaaatgca ctttttcaga aacgccgata cggtctcatc
      181 atgcatgcat tcttccactg tctgcagtcc gtataaatac gcacccagta ccatggacgt
      241 atgtccgccg ttcaaaatgc gcacttttcg gcgtttatac ggctgcacat catccgtcca
      301 tacaatatga tagcctgcct tattaaacgg cagttctttt tcgtgatttc cctcgatcac
      361 ccacagatga aaaatttccg ccgtatccag acaatgatcc tgataaccga tctctttcca
      421 gagctcttct gcttcttctt tcggatatcc tgtaacaata cggtctacaa gcgtattgca
      481 gaaatcattt tcttcatcga gccatgtcag gaatgcctgc ggaagttccc acagctttgc
      541 atattggatc acgcattcct ttaaatgatc cgcatttttg tcgatcaatt cacatggcat
      601 cagaataaat ccttttaacc ccagttcaaa acgtctgtac aacagctggg tcagctttgc
      661 cggataggag cctgcaggcc tgtcggaaag cttttctgtt ccccggtact cgatccctgc
      721 ttccgtcgta ttggaaataa taaaacggat atccggattt tccgccagct tcctatattc
      781 ctcaaattcc tcgtatggat tgatacatct tgaaatcacg tcaatatacg tgcgctcgtt
      841 caccatctgt ccgttttcaa tgccgcgcag atataaatga tatgcacagt tctgcgcaga
      901 gagcagttca cacatccctt tttcgatcgg ctgaacgacc accactttac cttcccacac
      961 acccttctca tttaatttat ataaaaaata gtccacaaac ccgcgcagaa atccgccttc
     1021 tccaaattgg atcacttttt caactgccat catgaatccc tccctgatac ttatttaaac
     1081 caactatagc caaatacgct cagcgtctta cgcgtccgga accgtctcct ttcacaaggc
     1141 gttccacctc catacgcgac acaagattaa aatctcccgg aatcgtatgc ttcagtgccg
     1201 aagcagccac tgcaaattca agcgattctt ttatattttt ttcatcgagc aatccgcaga
     1261 tcacacctgc tgcaaaagaa tctccgcttc ccacacgatc aacgatcggc gtgatcgcat
     1321 atgtttttga atggtaaaat tctcccgttt tcccgtcaat catgcaggcc gaccagccgt
     1381 tatcagatgc cgaacgagac cggcgcagag aaccggcaat atagcggaat ccaaatttat
     1441 cagccatctg atgaaatacc ttcttgtatt ccatcaaatc cagctcaccc gtcacaacag
     1501 aagatgcctc tgtcttgaaa ccaagtgcca gctccgcatc ttcttcgttc ccaatacaca
     1561 catccacata ctgcatcaga tttgtcataa ccttctgcgc cttttgcgga ctccataatt
     1621 tttttctgaa attcagatca accgaaacca tcacgcccgc tgcctttgct gccctgcatg
     1681 ccgcttccgt cagctcagcc gcactgtctg acagtgcagg cgtaataccg gtaaaatgaa
     1741 accagtccgc attttcaaaa atcgcagaaa aatcaaacat gtctgccgat attgtggcaa
     1801 cagcgctgtt tgtacggtca tagataacat tgcccggcct cattgccgat cctgattcca
     1861 aataataaat accaaggcgc tctccgtcct ttataatgaa atcacagccg acattatatt
     1921 ttttcagtga ggcgaccgca cagtcgccga tcggattatc cggcaccgct gttacatact
     1981 gcgtatcata tccgtaattg gcaagagaaa ctgcgacatt tgcctcaccg cccccataac
     2041 agacatcaaa ttcatctgcc tgtataaacc gctcatttcc cggcgtagac agacgcagca
     2101 tgatctctcc c
//
LOCUS       DAOSYE010000393         2110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_42756_length_2110_cov_2.783942, whole genome shotgun sequence.
ACCESSION   DAOSYE010000393 DAOSYE010000000
VERSION     DAOSYE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42756_length_2110_cov_2.783942"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLU43_07305"
     CDS             complement(<1..504)
                     /locus_tag="PLU43_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28256.1"
                     /translation="MNKKTNQILEEAKRSIFIVPSVLLLVVFFVTPILLTVYYSFTNL
                     ALSGENARQLKFIGLTNYRNMFKDRTVRVSIINTLIFLLGSLIGQQVLGFIIAVLMKN
                     KNRGFRGVIGPVFLAGWIMPEVVVALCCSTFFGDEGTLNSIFTFLHLPNVEFLYTIPM
                     ITVILANV"
     gene            complement(590..2035)
                     /locus_tag="PLU43_07310"
     CDS             complement(590..2035)
                     /locus_tag="PLU43_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO28257.1"
                     /translation="MKMKKLSALMMAAVMTLSLAACGSTSDTEENGSDTASASESAET
                     AEAEETTEAAETADNSAAEEKTKLTITFRDDGSDVLKNWFQNAYDTYDKKDSVELDIA
                     PITASEGDYFAKVALALQSEDTAPDVVCEDTFQLPSDVAAGYLANLSDYLKDYDEWND
                     GIFYGALVDGVTYDDGSVYGIPYCTDTRGLWYNKDIFAEAGLDTEWQPETWQDVIDTC
                     AVIKEKCPDVVPFWCNSGVATGEATSMQTYEMLLYGTGEQLLDDNDKWIVSSDNILKS
                     LNFISTIYTEGYGPSLSKVLNGEAGNTASREYLPNGKLAISLDGYWMTGNYKDTGAAP
                     WPEYEEKLGIAAMPTSEGQDPGSITMSGGWALSIPELSDNKDVAMDFIKHCMSYDVYL
                     DAIISQGNIATRTDIASDETYSSQPFMEKCTGFLDGAFYRPRNSQYSTVTTHIQTMVE
                     SVVSGTSPEDAMAQYKSDVTNSVGEENVVEK"
BASE COUNT          538 a          532 c          382 g          658 t
ORIGIN      
        1 cacatttgcc agaatcaccg ttatcattgg aatcgtatac aaaaattcaa catttggtaa
       61 atgcaggaac gtgaatatac tgttcaatgt tccttcatct ccgaaaaagg tactgcagca
      121 tagcgccaca actacttccg gcatgatcca tccggcaaga aatacaggcc ctatcactcc
      181 cctgaatccc ctgttcttat ttttcataag cactgcaata ataaatccca gcacctgctg
      241 tccgatcaga ctgcctaaca aaaagatcag cgtattgata atgctgaccc gtaccgtgcg
      301 gtctttaaac atattccggt aattcgtcag tccgataaac ttcaattgtc tggcattttc
      361 tcctgataat gccagattcg taaaggaata atataccgtt aataaaattg gtgtcacaaa
      421 aaacacaaca agaaggagga cagaaggcac tataaaaatt gatctctttg cttcttccag
      481 tatctgatta gtttttttgt tcattgcgtt cctccttaat aatagatata aaaaggaagg
      541 ctgggtgctt agatattgca cttcgccctc ctgcttttat ttcatccttc tatttttcta
      601 ccacgttttc ttccccgaca ctgttggtca catctgattt atattgtgcc atcgcgtctt
      661 ccggactcgt ccccgatacg actgattcga ccattgtctg aatatgcgtc gtaactgttg
      721 aatactggct gtttcttggt ctgtaaaaag caccgtcaag aaagcccgta catttttcca
      781 taaaaggctg tgacgaatat gtctcatctg atgcaatatc tgtccttgtc gctatattac
      841 cctgcgaaat aatagcatcc aggtaaacgt catagctcat gcagtgtttg atgaagtcca
      901 ttgcaacgtc tttattatcg gaaagctccg gaatcgacaa tgcccagccg cccgacattg
      961 taatacttcc cggatcctgg ccttctgatg tcggcattgc cgcgatcccg agtttttctt
     1021 catattccgg ccacggagca gcgccggtat ctttataatt tcctgtcatc cagtatccgt
     1081 caagagaaat tgcaagcttg ccgttcggca gatattccct tgatgccgta ttgcctgctt
     1141 ctccgttcaa tactttagac agagacggac cgtagccttc cgtatagatc gtactgatga
     1201 aatttaagga ctttaaaata ttgtcgctgg aaacgatcca tttgtcatta tcgtccagta
     1261 attgttcccc tgttccgtaa agaagcattt catatgtctg catggaagtc gcttctcctg
     1321 ttgcaacacc tgaattacac cagaacggaa caacatccgg acatttttct ttgatgactg
     1381 cacacgtatc aatcacatcc tgccatgttt ccggctgcca ctcagtatca agtcctgctt
     1441 ctgcgaatat gtctttgtta taccagagcc cgcgcgtatc cgtacaatat gggatcccat
     1501 atacgctgcc gtcgtcatag gttacaccat cgaccagtgc accgtaaaat attccgtcat
     1561 tccactcgtc ataatccttt aaatagtcgg aaagattcgc cagatatccg gctgccacat
     1621 cagacggcaa ttggaaggtg tcttcacata caacatccgg cgccgtatct tctgattgca
     1681 gagccaatgc aacttttgca aaatagtctc cctcagatgc agtgatcggt gcaatatcca
     1741 gctcaacaga atctttttta tcgtacgtat cataagcatt ttggaaccag ttctttaaaa
     1801 catcactccc gtcatcacgg aatgtgatcg taagctttgt tttttcctct gctgccgaat
     1861 tgtcggctgt ctctgctgct tctgttgttt cctctgcttc ggctgtctct gccgattctg
     1921 aagctgaagc agtatccgac ccgttctctt ctgtgtccga tgtactcccg caggcggcca
     1981 aagataatgt cattactgct gccatcatca atgctgataa ctttttcatt ttcatataat
     2041 accctctcct ttttctttgg tagtgtcaaa tctactacct tttctttttc ttaaaacatt
     2101 atattttcaa
//
LOCUS       DAOSYE010000394         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43016_length_2103_cov_3.123535, whole genome shotgun sequence.
ACCESSION   DAOSYE010000394 DAOSYE010000000
VERSION     DAOSYE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43016_length_2103_cov_3.123535"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="PLU43_07315"
     CDS             complement(<1..2094)
                     /locus_tag="PLU43_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOO28258.1"
                     /translation="MPIVMLGIHRLVQNPKKSKLYIITLAVSILMNYYLGYMLCLFSV
                     FYFLYILVINTTFKKHKRIYLKKIGSFLIASLLGVGLAAFDLIPIVISLKGQKSMPDR
                     SVLSFYRNFCFRDVFSKLYTNAFDGNVSNSSMPYIYTGILAAAFLILYFFNKKIAKKE
                     RIASAVFLVLMLGFCYIHTTDVIWHAFNEPVGFAYRYAFYISFLILHIGYQGYLHYEG
                     NRWSFLPVILIFISYSVYLILFGSKTLPRSAYILDMSILLLLGILIGIQKNYVLPFQL
                     GFLLILCLQCADLFENAVWSLKQYESITQTEYSAYIDRVKPVVEKIKEEDTSLYRIEK
                     NFTRNLNDSMQFSYNGLSHNSSCEKDYVKEFAANMGFRNFGIWAFYNEGSTSFTDCFL
                     GVKYFISKYDTTNKPYTADFNTEDTYVFKNPYVLPLAFSMEEQVRDLEINQSDTNDKD
                     PFEIQNKIAACFGSAAPVYTKAQVSDRTLENLTEEFSGEGDKGYTVYKKIDEKKEAFI
                     EYEITAAGNDILYYYFSAPDYQGAEIYVNDFSYGDYFSNWRWNIVNAGNYQKNEKIKI
                     RLEVKEEELKLYNSYFYEEDQNALEEWYAAAAAGAGELQEISSSHLTGTIDLDKQKYL
                     VFSIPYEKDWVIKIDGTRIEQEEVLDALMAVKLPAGHHVIEMYYLPSGFIAGAIISII
                     SLLIVLCMQLKLIDKE"
BASE COUNT          689 a          426 c          338 g          650 t
ORIGIN      
        1 ttctttgtca atcaatttca actgcataca taaaacaatc agcagggaaa ttatagaaat
       61 tatggcaccg gctataaatc cggaaggaag ataatacatt tcgatcacat gatggccggc
      121 cggcagtttt actgccatta atgcatcaag gacttcttcc tgttcgatcc tggtaccatc
      181 gatcttaatc acccagtctt tttcatatgg aattgaaaat acaagatatt tctgtttatc
      241 cagatcaatt gtgcctgtta aatgagaact tgatatttcc tgcaattcac cggctccggc
      301 tgctgccgca gcgtaccatt cttcgagagc attctgatct tcctcataaa aatagctgtt
      361 atatagtttt aattcctcct cttttacctc aagacgaatc ttaatttttt catttttctg
      421 ataatttccg gcattaacaa tgttccagcg ccagtttgaa aaataatctc cataagaaaa
      481 atcattcaca taaatttctg caccctgata atccggtgcc gaaaaataat aatataaaat
      541 atcattgccg gccgctgtga tttcatattc tataaaggcc tctttttttt catcaatctt
      601 tttatagacc gtatagcctt tgtctccctc tccggaaaac tcttccgtca gattttcaag
      661 tgtcctgtct gagacctgcg ctttcgtata aacaggtgcg gcagaaccaa aacaggcagc
      721 tattttattt tgaatttcaa agggatcctt atcatttgta tccgattgat ttatttccag
      781 atcccgaacc tgttcttcca tactaaaggc taatggcagt acatacggat ttttgaatac
      841 ataggtatct tccgtattaa aatctgctgt atacggttta tttgtcgtgt cgtacttgga
      901 aataaaatat ttaacaccta aaaaacaatc tgtaaaagaa gtacttccct cattataaaa
      961 cgcccatatt ccaaaattcc gaaagcccat atttgcagca aattctttta cgtaatcttt
     1021 ttcacaggaa ctgttatggg aaagtccgtt ataagaaaac tgcatggaat cattcaggtt
     1081 tcttgtaaaa tttttttcaa tccggtacag agacgtatcc tcttctttga tcttttcgac
     1141 aaccggtttt accctgtcga tatatgcgct gtattctgtc tgtgttatac tctcatactg
     1201 ttttaacgac catacggcat tttcaaataa atctgcacac tgcagacata aaataagaag
     1261 aaaaccaagc tgaaacggca aaacataatt tttctgtatt ccgatcaaaa ttccaagcag
     1321 taacaggata ctcatatcaa ggatataagc tgatcgcgga agcgttttac ttccaaataa
     1381 gatcaaatat acggaataac taataaaaat caatataaca ggcaggaacg accatctgtt
     1441 tccctcataa tgcaaatagc cctgatatcc gatatgaaga attagaaagc ttatatagaa
     1501 cgcataccgg taagcaaatc caaccggttc gttaaacgca tgccaaataa catctgttgt
     1561 atgtatgtaa caaaaaccaa gcattaaaac caaaaaaaca gcggaagcaa ttcgttcttt
     1621 ttttgcaatt tttttgttga aaaaatacaa aatcaaaaaa gctgctgcca gtatccccgt
     1681 ataaatatag ggcatgctgc tgtttgatac gtttccgtca aacgcatttg tatagagttt
     1741 tgaaaatacg tcccgaaaac agaaattccg ataaaaagac agcaccgaac gatccggcat
     1801 acttttttgt ccctttaggg aaatcacgat cggtatcaga tcaaaggcgg ccagtccgac
     1861 tccaagcaag gatgcaataa gaaaggatcc tattttcttt aaataaatcc ttttgtgttt
     1921 tttaaaagtt gtgttgatca caagaatata taaaaaatag aaaacggaaa acaggcagag
     1981 catataacct aaataataat tcatcagaat agatactgcc agtgttatga tatacaattt
     2041 gcttttttta ggattttgaa ccagcctgtg aattccaagc ataacgatcg gcagcaggat
     2101 aat
//
LOCUS       DAOSYE010000395         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43047_length_2102_cov_2.787005, whole genome shotgun sequence.
ACCESSION   DAOSYE010000395 DAOSYE010000000
VERSION     DAOSYE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43047_length_2102_cov_2.787005"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLU43_07320"
     CDS             complement(<1..245)
                     /locus_tag="PLU43_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HOO28259.1"
                     /translation="MFVQNGTGILKRVLLSPPAYLKPAPINEIAKQWKDTALDPVKME
                     REHKELIRVYEEQGVRTELLDPDPERPNAVFARDFGA"
     gene            complement(258..1931)
                     /locus_tag="PLU43_07325"
     CDS             complement(258..1931)
                     /locus_tag="PLU43_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO28260.1"
                     /translation="MDKSRFEEIEELTKKLVGIPSINGTEGEREIGEFLYSYIREIPY
                     FKEHPEHLYMQELKDDRLKRKNVFALVKGRAAALDGSGGNTILFHGHIDTVGVQDFGK
                     LSENAFSPDTLMEKLREMDLPPEVKQDLETGDYMFGRGACDMKSGDAVFLVLLKEFAA
                     VFEKLKGNLLFSFNPVEENAHTGIIRSLAFLNSLKEKEKLNYLMAINNDYICPLYPGD
                     TNRYVYTGAVGKILPCFYIQGKETHVGQCFEGVDASMLASILIRNINENPAYSDGYEG
                     EYTLPPSVLKVRDGKKWYNVQTAKDAFLYFNYFIHNASMEEILEQLVRAGKAAFEELE
                     KTVDQRYREYCELAGIQYSPWKMEKKVYTYQELFEIVWNRTKKADREELLAEIERIVQ
                     EDQKEGLDDREISAKVTSFLLRTAKITWPCIVIFFAPPYCPHNTLSGLAENEKKIKED
                     LKKITDKTGEKFGEQYQILNFFPSLSDSSYLKLDDSPESIECLVGNFPQQKQIYPIPF
                     EEIRKMNIPAVNYGCYGKDAHKWTERVYKPYSFGVLPELIRETVTYYLM"
BASE COUNT          534 a          544 c          350 g          674 t
ORIGIN      
        1 caggctccaa agtctcttgc aaacacggca ttcggccttt caggatccgg gtcaagcagc
       61 tctgttctga ctccctgctc ttcatacact ctgatcagct ctttgtgttc cctctccatt
      121 tttaccggat caagcgcagt atctttccac tgctttgcga tctcattgat cggtgccggc
      181 ttcagatatg cgggcggtga caataatact ctttttaata ttcccgttcc attttgtaca
      241 aacatatgct tctcctttta cattaaatag taggtgactg tttccctgat cagttccggc
      301 agcacgccaa atgaatacgg cttataaacc cgttccgtcc atttatgcgc atcctttcca
      361 tagcacccgt aattgacagc cggaatattc atctttctga tttcttcaaa cggaatcgga
      421 tagatctgct tctgctgcgg gaaattgccg accaggcatt ctatggattc cggtgaatca
      481 tccagcttta aatagctcga atcagacagg cttggaaaga aattgagtat ctggtattgt
      541 tccccaaatt tttctcctgt tttatctgtg atcttcttta aatcctcttt tatcttcttt
      601 tcattttccg ccaggcctga taacgtatta tgcggacaat agggcggagc aaagaaaata
      661 acaatgcacg gccatgtgat ctttgccgta cgcagcagga aggacgtcac ttttgcggaa
      721 atttccctgt catcaagacc ctccttctga tcctcttgca cgatcctttc gatttcagcc
      781 agcaactctt ccctgtctgc cttcttagtc ctgttccata cgatctcaaa cagctcctga
      841 tatgtataga cctttttctc catcttccat ggggaatact gaatacctgc cagttcgcaa
      901 tattccctat atctttgatc cactgtcttt tccagctctt caaaggcagc ctttccggca
      961 cggacaagct gctccaaaat ttcctccata gacgcattgt gaataaaata attaaaatat
     1021 aaaaacgcat cctttgcagt ctgcacattg taccatttct tcccgtccct gactttgaga
     1081 acactgggcg gcagtgtata ttctccttca tagccgtcac tgtacgcagg attttcattg
     1141 atatttcgaa tcagtatgga agccagcata gaagcatcga ctccctcaaa gcactgacca
     1201 acatgtgtct cttttccctg tatataaaaa cacggaagga tctttccgac tgctcccgta
     1261 taaacataac ggttcgtgtc tcccggatac agcgggcaga tatagtcatt attgatcgcc
     1321 atcagataat tcagcttttc cttttctttt aagctgttta aaaatgcaag gctccgtatg
     1381 atcccggtat gtgcattttc ctccaccgga ttaaaagaaa acaacagatt tccctttaat
     1441 ttttcaaaaa cagcagcaaa ttctttcagc agaactaaaa atacggcatc tccgctcttc
     1501 atatcgcagg cgcctctccc aaacatataa tctcctgttt ccagatcctg tttcacctca
     1561 ggcggaagat ccatttcccg taatttctcc atcagcgtgt ccggagaaaa cgcgttctcg
     1621 gacagctttc caaaatcctg gaccccgaca gtatctatgt gcccgtgaaa aaggatcgta
     1681 tttcccccgc tgccgtcaag agctgctgct ctgcccttta caagcgcaaa cacatttttt
     1741 cttttcaatc tgtcatcttt taattcctgc atatataaat gctccggatg ttctttaaaa
     1801 taagggatct ctctgatata tgaatacaga aattctccga tctcacgttc cccttctgtt
     1861 ccgttaatgc tgggaatacc taccagcttt tttgtaagtt cttcaatttc ttcaaatctg
     1921 gatttgtcca ttgtcctgct cctttacatt gatctgcctt tcaaaataaa aaggaaaccg
     1981 tcttcaaaat ttcagtaatt gaaatcttaa tccagctccc ttgcttctat tttattttaa
     2041 cagtccccaa aaaatattct atagcagtcc ctacaaaatt taaaaattat ttttgtaact
     2101 tt
//
LOCUS       DAOSYE010000396         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43081_length_2101_cov_2.840665, whole genome shotgun sequence.
ACCESSION   DAOSYE010000396 DAOSYE010000000
VERSION     DAOSYE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43081_length_2101_cov_2.840665"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLU43_07330"
     CDS             <1..357
                     /locus_tag="PLU43_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOO28261.1"
                     /translation="TIYKPVNKIAASFSSENTKSFKSVVTVEFPSQGIYSIGFITNDN
                     AAFGGDDKLCVFIPTTPNPTNGFLIFVEKDKVKELDLSVSDGLNMVVSIGSVIPQNIP
                     YKVSSETEGQQNGEAG"
     gene            complement(409..1041)
                     /locus_tag="PLU43_07335"
     CDS             complement(409..1041)
                     /locus_tag="PLU43_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HOO28262.1"
                     /translation="MQISSTTESTSASYSYGSAMNSGGTNQDEEIKSIKKQIVDLKQQ
                     IQSLSDDDELTDEEINEEKQELQQQIQDLNRQMSQRKTEIQQEKREAAADKAAKSGSD
                     ENAGGLSMDEQGVIISLSTKQEISEESTGSEETEETEETEETEATSGTEAASGSEEDT
                     ASQTKTENSVNKQSEDDSKQKTNLNDKPVIYTKDGKANQPEAEETLSVFV"
     gene            complement(1243..1635)
                     /locus_tag="PLU43_07340"
     CDS             complement(1243..1635)
                     /locus_tag="PLU43_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28263.1"
                     /translation="MEDKITCCIVDDSNFFRKFMRDIAEEMALTVLGDYHSGDSFLKD
                     LADESILFPELVFLDINMPGHSGTELLEDILDIVPEAVIIMISTVSDVKIVKECLMSG
                     ASNFINKNTDKKTIMEIITDTLRNNAML"
     gene            complement(1652..>2101)
                     /locus_tag="PLU43_07345"
     CDS             complement(1652..>2101)
                     /locus_tag="PLU43_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOO28264.1"
                     /translation="FKGLIDLIMDFVVRFFWWITDYGSSTIRLISVFLIATLGFAVVY
                     ALNPQVTNDIVLNESSNPLLVFIRALYFSVIIMTGLGLGEINAYEASYIGHIIILIQS
                     LSGYILLGAFLVRIGILFQGEFPVAAVRKKEGDHACDPDKSCVIKKQ"
BASE COUNT          548 a          503 c          389 g          661 t
ORIGIN      
        1 acaatctata agccggtaaa caagatcgct gccagctttt catcagagaa tacgaaaagc
       61 tttaaaagcg ttgtgaccgt ggagtttccg tcccagggta tctactccat aggctttatc
      121 acaaacgaca acgcggcatt cggaggagat gacaaactat gtgtgtttat ccctactaca
      181 ccgaacccca ctaacggatt cctgatcttt gtggaaaaag acaaagtaaa ggaactggat
      241 ctgtcagtat ccgacggatt gaacatggtc gtatccattg gttctgtgat tccacagaat
      301 ataccctata aggtctcttc cgaaacggaa ggacagcaaa atggagaagc cggctgaccg
      361 gcttctccga tgattatttt tgtatacaat aacgatccct gtgccggact atacaaatac
      421 ggagagggtc tcttccgctt caggctgatt tgccttgccg tccttcgtat aaatgacagg
      481 cttatcattc aaattggttt tctgtttgga atcatcctca gactgcttat tcacactgtt
      541 ttcggtcttg gtctgcgaag cagtgtcttc ttcgcttccc gatgccgctt ctgttcccga
      601 cgttgcttcc gtttcttctg tttcctctgt ttcttctgtt tcttccgagc ctgttgactc
      661 ctcactgatc tcctgtttgg tggaaagcga aataataacg ccctgctcat ccatacttaa
      721 tccgcctgca ttctcatcac tgccgctttt tgctgcctta tctgccgccg cttctcgttt
      781 ttcctgctgg atctccgttt ttctctgtga catctgcctg ttcagatcct gaatctgctg
      841 ctgcagttcc tgcttttcct cattgatttc ctcgtctgtc aattcatcat cgtctgaaag
      901 gctttgtatc tgctgcttca gatctacgat ctgttttttt atggatttga tttcctcgtc
      961 ctgattcgtt ccgcccgaat tcattgcgga accataactg taactggcag aagtgctttc
     1021 tgtcgtgctg ctgatctgca tatgtgtccc tccttggtct gattataact ggttgtcagg
     1081 tgatttctta tatatgttat cggttggttt cagaaaaatt gaagtggaga attgtgtcat
     1141 ttgtgttaaa aaaaagtgat gaaattgcaa aatgaaatac ccctgatact ttctttgcaa
     1201 tttcatcatt tttatagagc agctatgtga catgtcccat atttacagca tcgcattatt
     1261 tctcagcgta tccgttataa tttccatgat cgtcttttta tcagtatttt tattaataaa
     1321 atttgatgcg ccggacataa gacactcttt cacgattttg acatcgctga ctgttgagat
     1381 catgataata acagcttccg gaacgatgtc cagaatatcc tccagcagct ccgttcctga
     1441 atgtccgggc atattgatat caagaaagac cagctccgga aacaggatgc tctcatccgc
     1501 cagatctttt aaaaaactat ctccgctatg ataatctccc agcaccgtga gcgccatctc
     1561 ttcggctata tccctcataa attttcgaaa aaaattgctg tcatctacta tacaacaggt
     1621 aattttatct tccatgctct ctctcctgct cctattgttt ttttattaca cagcttttgt
     1681 ccggatcaca ggcatgatct ccttcttttt ttctgactgc tgccacagga aattccccct
     1741 gaaataagat gccgatcctg actaaaaaag cacccagtaa aatataacct gacaaagact
     1801 gtattaaaat aataatatgt cctatataag acgcttcata ggcattgatc tcccccaatc
     1861 caagccctgt cataatgatg accgaaaaat aaagcgctct gataaatact aaaagaggat
     1921 tagaagattc attcaggaca atatcgtttg tcacctgcgg attaagcgca tagacaacgg
     1981 caaatcccaa tgtagcgatc agaaagaccg aaatcagacg gatcgttgag gaaccataat
     2041 ccgtgatcca ccagaaaaac cgtacgacaa aatccatgat cagatcaatg agtcccttaa
     2101 a
//
LOCUS       DAOSYE010000397         2094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43327_length_2094_cov_2.394311, whole genome shotgun sequence.
ACCESSION   DAOSYE010000397 DAOSYE010000000
VERSION     DAOSYE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43327_length_2094_cov_2.394311"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLU43_07350"
     CDS             <1..606
                     /locus_tag="PLU43_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO28265.1"
                     /translation="LYVNQESAGFSDYLKEFQSTRELRNKKMCARLREHGIDITPEEL
                     KKEFPGAVITRGLYAKYMLKNGYVKSMKEAFDRYIGDHAPCYVPREKITPEQAVGFIK
                     SAGGLSILAHPTLYHMSRTRLETLVGRLKEAGLDGIEAKYTTYSISEEREMKEIAAKF
                     GLAISGGSDFHGTTKPDTDLGVGYGRLYIDDSVLENLKKIR"
     gene            623..1444
                     /locus_tag="PLU43_07355"
     CDS             623..1444
                     /locus_tag="PLU43_07355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOO28266.1"
                     /translation="MTADKKILFADLDGTLLNSEKNIAENDLRAIREMTANGHKFVIA
                     TGRPIQSAIHIAQKYDFITPGFYIASFNGGLIYDCYEKKTIRKYTVPFSSVSYLFREA
                     RKADLHCHTYSRTHVISEHDTDALRHYTAHIGMPPLVVDHILDALEEEPIKLIVISLD
                     SRKKLDDFRKKHASWEKGKLSSTFSSDILLEYANPASTKGNAVLFLCDYLHIPIENSI
                     AVGDEENDITMIDAAGLGVVMQNGTDRTKQHADYITEHDNNHNGIEEIIKKFMPN"
     gene            complement(1470..1742)
                     /locus_tag="PLU43_07360"
     CDS             complement(1470..1742)
                     /locus_tag="PLU43_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HOO28267.1"
                     /translation="MQEQQEYDDEVLQAFLENQNRLFDEDVANTLEEAEAFLEDCMAV
                     VVDSIEEVREYFEENGMDIDGMDMDELEDASEVFKVPDGRYLIVEG"
     gene            complement(1754..>2094)
                     /locus_tag="PLU43_07365"
     CDS             complement(1754..>2094)
                     /locus_tag="PLU43_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOO28268.1"
                     /translation="YHKGSIRDIGERILVPKWKDLCKACMAGEEWVDFMDASVDKGNA
                     LKTIQDFFHISKAETVVFGDNANDLGMLKRAEKSYVVANARDEVKRHAKYICAPYSEK
                     GVYRELQNYF"
BASE COUNT          614 a          529 c          412 g          539 t
ORIGIN      
        1 ctttatgtca accaggaatc cgccggtttt tcagactacc tgaaggaatt tcaaagcaca
       61 agagaactgc gcaacaaaaa aatgtgtgca cggctgcgtg aacacggcat agacatcacg
      121 ccggaggaac tgaaaaaaga atttccggga gcagttatca cccgtggtct ttacgctaaa
      181 tatatgctga agaacggtta tgtgaaaagc atgaaggaag cgttcgaccg ctatatcggc
      241 gatcatgccc cctgttacgt tccaagagaa aaaatcacgc cggaacaggc ggtcggtttt
      301 ataaaatctg ccggcgggct ttccattctc gcacatccga cactctacca tatgagccgt
      361 acacgccttg aaacacttgt cggcaggctg aaagaagccg gacttgacgg aattgaagcc
      421 aaatacacga cgtattccat tagtgaagaa cgggaaatga aagaaattgc cgctaaattc
      481 ggccttgcca tttcaggcgg ttccgatttt cacggcacga caaaaccgga cactgatctc
      541 ggtgtcggat acggccgtct ttatattgat gattccgtac ttgaaaacct aaaaaaaata
      601 cgataacttt ggagctgaaa aaatgactgc agacaaaaaa attttatttg cggatttaga
      661 tgggacactg ctgaattcag aaaaaaatat tgccgaaaat gatctgcgcg cgattcgtga
      721 aatgacggca aacggccata aattcgtgat tgcaacaggc cgcccaatac aaagcgctat
      781 tcatattgcg caaaaatatg actttatcac acccggcttt tatattgcca gcttcaacgg
      841 cggtctgatc tatgactgct atgaaaaaaa gacgatcaga aaatatacgg tgcctttttc
      901 cagcgtaagc tatctgttcc gtgaagcccg caaggccgat ctgcattgcc atacctattc
      961 acggacacat gtcatttccg aacatgacac ggatgctctg cgccactata cagcacatat
     1021 cggcatgcct cctcttgtcg tggaccatat tcttgacgca cttgaagaag aaccgatcaa
     1081 gctgatcgtc atttccctgg acagccgcaa aaagctggat gattttcgca aaaaacatgc
     1141 ctcctgggaa aagggaaagc taagcagcac tttttcaagt gatattctgc ttgagtatgc
     1201 caatcccgct tctacaaaag gcaatgccgt actgttccta tgtgattatc tgcatattcc
     1261 tatcgaaaac tccattgccg tcggcgatga agaaaacgat attaccatga tcgatgccgc
     1321 cggcctcggt gttgtcatgc aaaatggaac tgaccgcaca aaacagcatg cggactatat
     1381 taccgaacac gacaataacc ataatggaat tgaagagatc atcaaaaaat ttatgccaaa
     1441 ctgactaaaa acagcacgcc ggcagattcc tatccttcca cgatcagata tcttccgtcc
     1501 ggtaccttga atacttcgct ggcatcttcc agctcgtcca tatccatgcc atcgatatcc
     1561 atgccgttct cctcaaagta ctcccgtacc tcttcgatgg aatccacaac gactgccata
     1621 cagtcttcca aaaaagcttc tgcttcttcc agtgtattgg ccacatcttc atcaaaaagg
     1681 cggttctgat tttctaaaaa cgcctgtaac acctcgtcat catattcctg ttgttcctgc
     1741 atatcgatcc ctcctaaaaa taattttgaa gttccctgta tactcccttt tcactgtaag
     1801 gagcacagat atactttgca tgacgtttta cttcgtcacg ggcattcgcc acaacataac
     1861 ttttctcggc ccgtttcagc attccgagat cattggcgtt atcaccaaat acgaccgttt
     1921 ctgctttgga aatatgaaag aaatcctgaa tcgtctttaa tgcatttccc ttgtcgacag
     1981 aagcatccat aaaatcgacc cattcttctc ccgccataca tgccttgcac agatctttcc
     2041 attttggaac aaggatacgc tcaccgatat ccctgatgct acctttatga tagt
//
LOCUS       DAOSYE010000398         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43399_length_2092_cov_2.572901, whole genome shotgun sequence.
ACCESSION   DAOSYE010000398 DAOSYE010000000
VERSION     DAOSYE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43399_length_2092_cov_2.572901"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PLU43_07370"
     CDS             <1..2058
                     /locus_tag="PLU43_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28269.1"
                     /translation="LVIADTFLKRYIYEKTMGEQHATILKLYESEKKYNEKIEVYQKV
                     LEQSPVSVVITDTEGKIEYVNPYFSELTGYQLEEVLGKNPRILKSGKTDSAKYMELWK
                     KLKNGEEWRGEFVNLNKENQEYEESAIITPIKNADNHVTHYVSIGENITQRKQKEQEL
                     QNALIRAEEAAVAKSLFLANMSHEIRTPMNAVIGMAYLALQTDLSKKQADYISKIHKA
                     ATSLLGILNDILDFSKIEAEKLELECEEFNLDSTVLETIGLTTQAGYEKGLEFIYHLS
                     PEIPKQLLGDSLRLRQVIANLVSNAVKFTQKGKITIDIKMIGNTVKNTGEKIQLIFSV
                     VDTGIGIAEADLKKLFEAFSQTDNSMTKRFGGTGLGLAICKKLVSLMEGEIWVESIPK
                     KGRIFSFSASFKIADTCKEECCVFPSEIHNKRILIVGDSNEERYILAEYLKAMDFQTE
                     EASSAREAENFIKEADQTQFYDIVLVDWKMERMNGLELIRKINRELGLFHVPKVILLT
                     SCNREDIREQAGDISIHNILVKPIGQSMLYNAIVNIYLAEKKNTDTKVRVDTDICCLD
                     KISVLLVEDNEVNRQIASELLESQGMIVEIAQNGLEAVHKVENMPAEAPYHLILMDLQ
                     MPKMDGFEATRQIRKKNIQIPIIAMTARTLLEEQKECYKAGMNDHISKPIDIDLLFQT
                     IER"
BASE COUNT          751 a          348 c          468 g          525 t
ORIGIN      
        1 cttgttattg cagatacgtt tttaaagcga tatatctacg aaaaaacaat gggtgaacaa
       61 catgcaacca ttttaaaatt atatgaatca gaaaaaaaat ataatgaaaa aatcgaggta
      121 tatcaaaagg ttctggaaca atcgccggta tctgttgtta ttacggatac ggaaggaaag
      181 atcgaatatg tcaatcccta tttcagtgaa ctgacaggat atcaattaga ggaagttctg
      241 gggaaaaatc cacggatact caaatcggga aaaacagatt ccgcaaagta tatggaactt
      301 tggaaaaaac tgaaaaacgg agaagaatgg agaggggaat ttgtaaatct caataaagaa
      361 aaccaggaat atgaagagtc tgcaatcatc acaccaatta agaacgcaga caatcatgta
      421 acacattatg tttctatcgg cgagaacatt acgcagagaa aacaaaagga acaggaactc
      481 caaaatgcat tgatccgtgc ggaagaggct gctgtagcaa aatctctgtt tttggccaat
      541 atgagtcatg agatcagaac gccgatgaat gcggtaattg gaatggctta cctggcactg
      601 cagacagatc taagtaaaaa acaggcagac tacatatcaa agatccacaa ggcggcaacc
      661 tctttattgg gaattttgaa tgatatcctg gatttttcaa aaattgaagc tgaaaagctg
      721 gagctggaat gtgaagaatt caaccttgat tctactgttt tagagacaat cggacttaca
      781 acacaagccg gttacgaaaa aggcctggaa tttatatatc acctttcccc ggaaatacca
      841 aaacagctgt taggggattc cctgcggctg cgtcaggtaa ttgcgaattt agtgagcaat
      901 gctgtgaagt ttacgcaaaa aggaaaaatt acgattgata ttaaaatgat tggcaataca
      961 gtcaaaaata caggtgaaaa aatacagctt atattttctg ttgtggatac gggtataggg
     1021 attgcggaag ctgatttgaa aaaattattt gaagcttttt cccaaacaga taattcaatg
     1081 actaaaagat ttggcggaac gggccttggg cttgcgatct gtaagaaatt agtatccctg
     1141 atggaagggg aaatatgggt cgaaagcata ccaaaaaagg gacgcatctt ttctttttcc
     1201 gcatctttta aaattgcaga tacatgtaag gaagaatgct gtgtttttcc aagtgagatc
     1261 cataataagc ggatattaat tgtgggagat agtaatgaag aaagatatat actggcagaa
     1321 tacttaaagg caatggactt tcagacagaa gaggcctctt ctgcaagaga agcggaaaat
     1381 ttcattaaag aagccgacca gacacaattt tatgatattg tactggtcga ttggaaaatg
     1441 gaacgaatga atggccttga actaatcaga aagatcaaca gagaacttgg actttttcat
     1501 gtgccaaaag ttattttgct cacttcctgt aacagggagg acatcaggga gcaggccgga
     1561 gatatttcaa ttcataatat tctggtaaaa ccaatcggtc agtctatgct gtacaatgcg
     1621 attgtcaata tatatcttgc ggagaagaag aacacagata caaaagtccg cgtagataca
     1681 gatatctgct gcctggataa aataagcgta ctcttggtag aagacaatga agtgaaccgg
     1741 cagatcgctt ccgaattgct ggaaagtcag ggaatgatcg ttgagatcgc acagaatggt
     1801 ctggaagcgg ttcacaaagt ggaaaacatg ccggctgaag ctccttacca tctgatactg
     1861 atggatttac agatgccaaa aatggatggt tttgaagcga cgagacagat cagaaagaaa
     1921 aacatacaga taccaattat tgcaatgaca gccagaacgc tgctggaaga gcagaaagaa
     1981 tgctataaag caggaatgaa tgaccatatt tcaaaaccaa ttgatattga tctgctgttc
     2041 cagacgatag aacgctgaat accatctttt cataatcagg attctgctgc tg
//
LOCUS       DAOSYE010000399         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43439_length_2091_cov_2.503438, whole genome shotgun sequence.
ACCESSION   DAOSYE010000399 DAOSYE010000000
VERSION     DAOSYE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43439_length_2091_cov_2.503438"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLU43_07375"
     CDS             <1..533
                     /locus_tag="PLU43_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO28270.1"
                     /translation="GIILDISSQTDLLALNASVEAARAGEAGRGFAVVAEEIRKLAEQ
                     TRMATENITGLLDALSVNTTAVSNKVSDNVVIAENQKGLIDVTKDGFSKIDDEIRILS
                     QNINEVNERMEDLMDSNNKIVDAISNISATGEEISASTEQAFGASKENVSSIENFADI
                     MNEISEAVKELASYQI"
     gene            762..>2091
                     /locus_tag="PLU43_07380"
     CDS             762..>2091
                     /locus_tag="PLU43_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO28271.1"
                     /translation="MKTDYLVQKMENFFDKRTVLLKNEANDVSALIGAEDQKGALQQY
                     LIKQLENQKDDYGIIDVYVGYPDGTIDCGSGWIPDDPDWKANERSWYLLAEQAGGEIA
                     YTDVYIDSDTKKPVVTIAIALNESGKTEGVLAADVSLSQLDELMSSEKIGNTGYPFVL
                     DSDGRFLIHPTYEFNEEVEAADTIFNISNGTLVEVGNKLLSGEAELCKGSFNGVDKVY
                     YAVQIEGTQFYLVASITYEEIMSGLNKILMHEGIIALLAILLSTFLLMFFIRRITGAI
                     ERNVMQLRKLSAGELGIGEEVIDSKRKDEIGDIARATVELESALRNIISEVDQAAVSL
                     QTTSEKLEQVSAETTATTDGIERAVGDVARGALSQAQSTEEASRQSRIMGENIEDTVN
                     AIQQLNSNSDKMIKSSTAAMDSLNELNTINEKTKTEINTIYDQTNETNEFA"
BASE COUNT          696 a          329 c          529 g          537 t
ORIGIN      
        1 ccggaatcat attggatatt tcttcacaga ccgacctttt ggcgttaaat gcttctgtgg
       61 aagcagcgag agccggcgaa gcaggaagag gatttgcagt cgttgcggag gagatcagaa
      121 aactggcaga gcagaccaga atggcaacag agaatattac aggtctgctt gatgcgcttt
      181 ctgtcaatac cactgctgtt tccaataaag tttcggataa tgttgtgatt gctgaaaatc
      241 agaagggcct gatcgatgtt acaaaagacg gattttcgaa aattgacgat gagatccgca
      301 ttttaagtca aaatatcaat gaagtaaatg agcgcatgga agatctgatg gattcaaata
      361 ataaaatcgt tgatgcgatc agtaatattt cggcaacagg agaagagata tcggcgagta
      421 ccgagcaggc gtttggagcc agcaaggaaa atgtgtcttc aattgagaat tttgcggata
      481 ttatgaatga aatttcggaa gctgttaagg aattggcaag ctatcagata taaattgaat
      541 aattacagaa ccctacttct tttcatttaa aaatgaaagg ataaaagacc tgcagtgaat
      601 ttattatgag agggaaaacg ataatgagaa aaaacggact taacaaaaag tttattgtaa
      661 tgatatctgt gatactaata ttgacggtgc tatcggtctc gacatcttca tacagagata
      721 catctgttat agttaagaac caggtagatg aatatctgat tgtgaaaacg gattatcttg
      781 ttcagaaaat ggagaatttt tttgataaac gaacagtgct gcttaagaat gaagcaaatg
      841 atgtttcggc cttaatcggt gctgaagatc aaaagggggc tcttcagcag tatttgataa
      901 agcagcttga aaatcagaaa gatgactatg gtattatcga cgtgtacgtc ggttatccgg
      961 atggtacgat agactgcggc tcgggatgga tcccggatga tccggattgg aaggcaaatg
     1021 aacgttcctg gtatctttta gcagaacagg ccggcggaga aattgcatat acggatgttt
     1081 atattgattc ggacacaaag aaaccggtcg ttacgattgc aattgcactg aatgaaagcg
     1141 ggaaaacgga aggggtattg gctgctgatg taagtctttc acagcttgat gagctgatgt
     1201 cctcagaaaa gatcggaaat acggggtatc cttttgtact tgacagtgac ggcagatttc
     1261 tgatccatcc tacatatgaa ttcaatgaag aagtggaagc ggcagatacg atttttaata
     1321 tcagcaacgg gacattggta gaagtgggaa ataaattgct ttccggagaa gcggaattat
     1381 gtaagggttc gtttaatggg gtggataaag tttattatgc agttcagatt gaaggcacac
     1441 agttttattt agtagcaagt atcacatatg aagaaataat gtcgggattg aataagatcc
     1501 tgatgcatga aggtatcatt gcgctgcttg caatacttct ttcgacgttt cttctcatgt
     1561 ttttcataag aaggattacc ggtgccattg aaagaaacgt aatgcagctg cgaaaattgt
     1621 cggctggtga gctcggtatc ggggaagaag tgattgattc aaaacggaaa gatgagattg
     1681 gggatattgc aagggcgacc gttgaactgg aaagtgcact tagaaatata atatcggagg
     1741 tggatcaggc tgcagtgtct ttacagacta cttcggaaaa acttgaacag gtttctgcgg
     1801 aaacaacagc gacaacagac gggatcgaaa gagcagtggg agatgtggca cgaggagctc
     1861 tatcacaggc acagtccaca gaggaagcgt caaggcagag cagaatcatg ggagagaata
     1921 ttgaggacac agtgaatgca atccagcagc tgaattcgaa ttccgataaa atgatcaaat
     1981 ccagtacggc agcaatggac tcgctgaatg aactgaatac gataaatgaa aaaacaaaaa
     2041 ccgagatcaa tacaatatat gatcagacaa acgaaacaaa tgaatttgca t
//
LOCUS       DAOSYE010000400         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43466_length_2090_cov_3.342015, whole genome shotgun sequence.
ACCESSION   DAOSYE010000400 DAOSYE010000000
VERSION     DAOSYE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43466_length_2090_cov_3.342015"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1170)
                     /locus_tag="PLU43_07385"
     CDS             complement(37..1170)
                     /locus_tag="PLU43_07385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO28272.1"
                     /translation="MKRTADVIVIGAGIIGNAAAYYLAKKKMDVIVLEAGDSIGCGGS
                     SRNGGGVRQSGRDPRELPYAIYGIQHIWPTLSEELGTDVEYHQGGNLRLGLNEEHLKI
                     LQNFAKKAQNCGLDVKMVDGREVREICPYLSEEIVGASWCPTDGHANPMKTTLAYYKR
                     ARELGAVFVTGEAVLSIKKIAGRARKAITVNGNVYEADHIILAAGYESRAIAQTVGID
                     IPMSRYYEECLVTEMQPPMFDIMLGVATAEIYGHQCEHGSFVFGSGTGLERTFEFDNM
                     RNKCRALNASANCRVIMKYVPSLKDAKVVRVWGGWMDIAQDMVAVIGKTDEVPGLILA
                     CGMSGHGFGTGPIVGLMLSQLVLDEELPCDISQLRYDRFKSDR"
     gene            complement(1167..1496)
                     /locus_tag="PLU43_07390"
     CDS             complement(1167..1496)
                     /locus_tag="PLU43_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO28273.1"
                     /translation="MTKENVRLKDIGEFVPQEDDDLIICRCEEVTKGEIRRAVHDGMY
                     TLTEIRRYLRTGMGLCQGQTCARLVKGIVAKELQVAPEELEPALSRTPMRPIEIDILA
                     KDGGNRS"
     gene            complement(1500..>2090)
                     /locus_tag="PLU43_07395"
     CDS             complement(1500..>2090)
                     /locus_tag="PLU43_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HOO28274.1"
                     /translation="AEFEKALESLRQGMFAPKNRGKLLTKTEEGILISENLLKKGFVA
                     ESEIERFPGVTKRAGIHPVIECTQNIPCNPCQDACPKGCIKIGEKITSLPAVDESAVC
                     IGCGMCVASCSGQAIFLVDEEFEEGYASVTLPYEFLPLPEKGYMGKGMDRSGKEICDA
                     QVIDVKTAQAYDHTNLLTIKVPKDMAMKARFFKCEV"
BASE COUNT          490 a          614 c          369 g          617 t
ORIGIN      
        1 tttgtaatca gtattacgaa aaatcctgct taagttttat ctgtcacttt tgaaccggtc
       61 gtatcgcagc tgcgaaatat cacacggcag ttcttcatca agaactaact gcgacagcat
      121 aagtcccacg atcggacctg ttccaaaccc atgtccgctc ataccgcatg ccagaatcag
      181 acccggcact tcatctgtct taccgatcac ggctaccata tcctgcgcaa tatccatcca
      241 gcctccccag acacgcacga cttttgcatc ttttaaggac ggtacatatt tcataatgac
      301 cctgcaattg gccgaagcat tcaatgcccg gcatttattt ctcatattgt caaattcaaa
      361 cgtccgttcc aggccggtcc cgcttccaaa tacaaaagaa ccatgctcac actgatgccc
      421 atatatttcc gcagttgcaa ctccaagcat aatatcaaac atcggcggct gcatttccgt
      481 cacaagacat tcttcatagt acctcgacat tgggatgtcg attccaactg tctgcgcaat
      541 tgctcttgat tcatacccgg ccgcaagaat aatatgatcc gcttcatata catttccgtt
      601 caccgttatt gcttttctgg ccctccccgc tattttttta atggaaagca cagcttcgcc
      661 tgtaacaaat accgctccca gttctcttgc ccgtttataa taagccagcg tggttttcat
      721 cggatttgca tgcccgtctg tcggacacca gcttgccccg acgatttctt ctgaaagata
      781 cggacagatc tctctcactt ccctgccgtc gaccattttt acatcaagcc cgcagttctg
      841 cgctttttta gcaaaattct gcagtatttt cagatgctct tcgtttaatc caagcctgag
      901 atttccgccc tgatgatatt ccacatccgt accgagctcc tccgacaaag taggccagat
      961 atgctgaatt ccatagatcg catagggaag ttccctggga tctcttccgg actgacgcac
     1021 accgccgcca ttcctgctgg aaccgccgca gccaatgctg tcgcccgcct ccaaaacaat
     1081 gacatccatt tttttctttg ccagataata tgcagcagca ttccctatga ttcctgcacc
     1141 aataacgatt acatccgccg tacgcttcat gacctgttcc ctccatcctt tgccagtata
     1201 tcgatctcta tcggccgcat gggagttctg gacaatgcgg gttccagctc ttcaggtgca
     1261 acctgcagct cctttgcaac aattcccttt acaagccgcg cgcaggtctg tccctgacac
     1321 agtcccatgc cggtccgcaa ataccttctg atttcagtga gcgtatacat gccgtcatgt
     1381 acggctctgc gaatttctcc tttggtcact tcctcgcacc ggcaaatgat cagatcgtca
     1441 tcctcctgcg gaacaaattc tcctatatcc ttcaaacgca cattttcctt tgtcattttt
     1501 tacacctcgc atttaaaaaa tcgtgccttc attgccatat ccttcggtac ctttatcgtt
     1561 aacagattcg tatggtcata tgcctgagct gttttgacat caatgacctg cgcatcacat
     1621 atttcttttc cgcttctgtc cattcctttt cccatatacc ctttttcagg aagaggcaga
     1681 aattcatatg gtaatgtcac ggatgcatat ccctcttcaa attcctcatc tactaagaaa
     1741 atagcctgac ctgaacagga agccacacac atgccgcatc cgatacagac agcgctttca
     1801 tcaactgcag gcagagaggt gatcttttct ccaattttta tacagccctt cggacaggca
     1861 tcctggcatg gattgcatgg aatattctgt gtacactcta tgaccggatg aatgcccgca
     1921 cgttttgtta cgcctggaaa tcgttctatt tccgattctg ccacaaatcc ctttttcagc
     1981 aggttttccg atattaagat cccttcttcc gttttcgtca gcagttttcc cctgttcttc
     2041 ggggcaaaca ttccctgtct gagactttcc agtgcctttt caaattctgc
//
LOCUS       DAOSYE010000401         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43557_length_2087_cov_3.324311, whole genome shotgun sequence.
ACCESSION   DAOSYE010000401 DAOSYE010000000
VERSION     DAOSYE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43557_length_2087_cov_3.324311"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLU43_07400"
     CDS             <1..1192
                     /locus_tag="PLU43_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOO28275.1"
                     /translation="IEEAVLYGKSGNPVVRYKEQKDLEQEKKVYALDLQFDRTAIRSA
                     VEAQGEQYNVEAIEASMTKEDDGFQIVSGQIGYIVNEDASVNLIAEALTSWDGQPAEI
                     RLQVDVAEPKGTAEELSLVKDVLGTYTTSFSSSATERSGNVRNGTKLINGTLLYPGDT
                     FSAYETVSPFTEENGYYLAGSYLNGMVVESLGGGICQVSSTLYNAVIRAELQVDERYN
                     HSMIVGYVQLSADAAISGTYKDFKFTNSSEYPIYIEGYTTDDKKITFTIYGVETRPAN
                     RTIEFESVELSKTEPEGEKVIEDSAQPAGYISVQSAHIGYVGELWKVVKIDGTETERT
                     QLNKSTYTASPKTATVGTAASDPSITTMLQAAIATGSIDYCKSIISQLNAAAADAVAA
                     GAAQ"
     gene            1214..>2087
                     /locus_tag="PLU43_07405"
     CDS             1214..>2087
                     /locus_tag="PLU43_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOO28276.1"
                     /translation="MNIGKVPENILKRSIIKQIKTKRKEILVGAGVGEDCAVISLAAD
                     EVFVISTDPITGTSHDIGSLSVHVTANDIASSGAEVIGVMLSVLLPENTEEAELKDMM
                     RQVEETCAKLHIQTIGGHTEVTKAVSQAIITVTGVGKVKKDRVITTSGARPGDDVVVT
                     KWIGLEGTSIIAKEKEQELLSKFAPSFVEKAKSFSDYLSVVPEAAVAAKSGVSAMHDV
                     TEGGIFGALWEVAEASGTGLEIDLKKIPVRQETIEVCEYFGLNPYTLISSGSMLIAGP
                     AGHDRSGRPGGGRCA"
BASE COUNT          570 a          422 c          612 g          483 t
ORIGIN      
        1 aattgaagag gctgttttat acggaaaatc cggcaatccg gtagtccgct ataaagagca
       61 gaaggatctg gaacaggaga aaaaagtata tgcgcttgat ctccagtttg acaggaccgc
      121 gatcagatct gcagtggaag cgcagggcga acagtataac gtggaagcga tagaggcttc
      181 catgacaaag gaagacgacg gcttccagat cgtttcagga cagatcggat atatcgtgaa
      241 tgaggatgcg tccgtcaatc tgatagctga ggcgcttact tcatgggatg ggcagccggc
      301 tgagatccgc ctgcaggtgg atgtggccga gccgaaagga acagcagagg agctttcttt
      361 agtcaaagat gttcttggta cctatacgac gagtttttct tcttccgcaa cagagcgttc
      421 gggaaatgta cggaacggaa cgaagctgat caacggaaca ctgctttacc cgggagatac
      481 gttttccgca tatgaaacag tcagtccgtt tactgaggaa aacgggtatt atctggcagg
      541 ctcctattta aatggtatgg tagtggaaag tcttggcggc gggatctgtc aggtgagctc
      601 cactctttat aatgcggtta tccgtgcgga gctgcaggta gatgagcgct ataatcattc
      661 catgatcgta gggtatgtac agctgtctgc ggatgctgcc atttccggga cttataagga
      721 ttttaaattt acgaacagct ctgaatatcc gatttatatt gagggctata cgactgatga
      781 taaaaaaatt acatttacaa tttacggagt agagacaaga cctgcaaacc gtacgatcga
      841 atttgaaagc gtggaactta gcaaaacgga gcccgaaggg gaaaaggtga tcgaggacag
      901 tgcacagccg gccggttata tcagtgtcca gtcggcacat atcggctatg tcggtgaact
      961 ctggaaagtt gttaagatcg atggaacgga aacagagcgt acacagctga ataagagtac
     1021 gtatacggca tctccgaaaa cagctacggt aggaacggca gcgtccgatc cgagtattac
     1081 gactatgctg caggcggcga tcgctacggg cagtatcgat tattgcaaga gtattatttc
     1141 ccaattgaat gcggcggccg cagatgctgt ggcggcagga gccgcacaat aatgaaaggg
     1201 acggatatgt gacatgaaca tcgggaaagt acctgaaaat atattaaagc gttcgatcat
     1261 caaacagatc aaaacaaaga ggaaagagat ccttgtcgga gcaggcgtcg gtgaagactg
     1321 tgcggttatt tcgcttgctg ctgatgaggt ttttgttata tctacggacc cgattaccgg
     1381 aacatcccat gacatcggtt ctttatccgt tcatgtgacg gcaaatgata ttgcgtcctc
     1441 aggggcggaa gtgatcggag tcatgctgag cgtgctgctt ccggaaaata cggaggaagc
     1501 ggagcttaag gatatgatgc ggcaggtgga ggaaacctgt gcaaagctgc atattcagac
     1561 gatcggcgga cataccgaag tgacgaaagc ggtcagtcag gcgattatca cggtgaccgg
     1621 tgtcggaaag gttaaaaagg acagggtcat tacgacttcc ggggcgagac cgggagatga
     1681 tgtggtcgtg acaaaatgga tcggacttga aggcacttcc attattgcaa aggaaaagga
     1741 acaggagctt ctttctaaat ttgccccttc ttttgtggaa aaggcaaaaa gtttttccga
     1801 ttatctgtcg gttgtgccgg aagctgctgt ggcggcgaag tccggcgtca gtgcaatgca
     1861 tgacgttacg gagggcggca tcttcggggc tctttgggag gtcgcggaag catccggcac
     1921 cggacttgaa atcgacttaa aaaagatacc ggtcagacag gagacgatcg aggtctgcga
     1981 gtatttcgga ttgaatccgt atacattgat ttcgagcggt tccatgctga tagcgggtcc
     2041 ggcaggacat gatcgatctg gtcggcccgg cggtggacgt tgcgccc
//
LOCUS       DAOSYE010000402         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43564_length_2087_cov_2.889272, whole genome shotgun sequence.
ACCESSION   DAOSYE010000402 DAOSYE010000000
VERSION     DAOSYE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43564_length_2087_cov_2.889272"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="PLU43_07410"
     CDS             complement(<1..2066)
                     /locus_tag="PLU43_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOO28277.1"
                     /translation="MYLDSDDVLHYTLTLNGYKFDFDENGDITGCEPQSGADTDLVIP
                     DTIGDISITTIGDSAFSGLSGTTTTITIPDTITTIGDNAFENCSTIEEVYINTKGVAI
                     GASAFADNASLTGVYFTQTEGSGETTLGAACFKNCPDMEIIQLRNDDFTGDSINDVNI
                     TSIGSEAFKTGRTSPYTDSDLDIDNIFLLIKGKMDTTYAPYAFAIDGNNTVSTINNSY
                     VTYFSGNPQNLVARYSKTLNDSAGGVSVLKYPGMNTSVGTVSGSSVTVHDLVDKVSTG
                     ELITVNQQDIIDNTTAMSIPNGIDSIDNASNDLDGTNSYFRKVNDPYDEGNGLQTINL
                     YGVTALPEDGQSFSENEDLTAVTFHSDVTDLSSIPFYLSTSLASITFAGEGTYEDATT
                     EDPYYWCTDGIIFSYDGTDTTIEEVLAGRGSVVGETSISSTETADVTKIAESAFMDCD
                     IIREVDLSGSEGLTEIPENCFYGCDQLTDVILPESVNQIGDAAFADGNDYISVTIPST
                     EVDIADDAFSNDLYATIWSYDDSAAQRYATRKGITFKLLDSLYTVKFADYDGTILKTE
                     RVEAGEDATPPTTDPTRTGYTFTGWNPTYLDITEDTTCVAQYTSTTTSTSSSSTSSSS
                     SSSSSTSTSSSSTSTSSSSSSSSSSSSSSAVVSTTITSSTAAPIVISGTGTSGGTGSS
                     GTTGSGT"
BASE COUNT          515 a          541 c          436 g          595 t
ORIGIN      
        1 gtcccgctgc cggttgtccc gctgctgcct gttccgccgg aagtaccggt accgctgatc
       61 acgatcggtg ctgccgtgct gcttgtgatc gtagtagaga ccactgcaga actggaactc
      121 gaactggaag agctggaact tgagctggac gttgaagtgg aagaactgga tgttgaagtt
      181 gaactcgaac tggagctgct cgatgatgta cttgacgatg aagttgaggt cgttgtactc
      241 gtatactggg caacacaggt cgtatcctcc gtaatatcca gataagtcgg attccaccct
      301 gtaaacgtat aaccggtcct tgtaggatca gtcgtcggcg gtgtcgcatc ctctccggct
      361 tcgacccgct ctgtcttgag tatggttccg tcataatccg caaattttac cgtatacagg
      421 gaatccagca atttaaacgt aattcctttt ctagttgcat agcgctgtgc agcactgtcg
      481 tcataactcc agatcgtcgc atacagatca ttggaaaacg cgtcgtctgc aatatccact
      541 tctgtagaag gaatcgttac actgatataa tcgtttccat ccgcaaatgc agcatccccg
      601 atctggttga cagactccgg aagtattaca tccgtaagct gatcacatcc ataaaaacaa
      661 ttttccggta tttccgtaag tccttcagat cctgacagat ccacttctct tataatatca
      721 caatccataa aggcactttc agcgatcttt gttacatccg ctgtctctgt cgagctgatc
      781 gacgtctccc caactacact tccacggcct gcaagcactt cttcgattgt cgtatccgtc
      841 ccgtcatagc tgaagatgat cccatccgta caccagtaat acgggtcttc cgtcgttgca
      901 tcctcataag ttccttcccc ggcaaaagtg atgcttgcca ggctcgttga taaataaaac
      961 ggaatggaac ttaagtcagt tacatcagaa tgaaacgtca ccgcagtcag atcttcattt
     1021 tcggaaaaag actgcccgtc ttccggcaaa gcggtcaccc catataaatt gatcgtctgc
     1081 agtccgttgc cttcatcata cggatcattt acttttctga aataagaatt tgtaccgtca
     1141 agatcattgg atgcattatc tatggaatcg atcccgttcg gtatactcat agcggtcgta
     1201 ttatcgataa tatcctgctg attaaccgta atcagttctc ctgtgctcac tttatcgacc
     1261 agatcgtgca cggtaacaga gcttccggac accgtaccaa cacttgtatt catccccgga
     1321 tattttaaaa cagacacacc gcctgcgctg tcatttagtg ttttgctgta ccgtgcaacc
     1381 agattctgcg gatttccgct gaaataagtc acatagctgt tattgatcgt actgaccgta
     1441 ttgtttccgt caatggcaaa cgcatatggt gcataagtcg tgtccatctt tcccttgatc
     1501 agcagaaaaa tattatcgat atcaagatcc gaatccgtat acggagatgt ccttcctgtc
     1561 ttgaatgcct cgcttccgat cgatgtaata ttgacatcat tgatgctgtc gccggtaaaa
     1621 tcatcgtttc gcagctgaat gatctccata tccggacaat ttttaaaaca ggcggcgccc
     1681 agtgtcgttt caccgcttcc ttctgtctgg gtaaaataaa cacctgtcag ggaagcatta
     1741 tctgcaaacg cactggctcc gatcgcgacc ccctttgtat tgatatagac ttcctcgatc
     1801 gtcgaacaat tttcaaacgc attatctccg atcgtcgtga tcgtatccgg gatcgttatt
     1861 gtcgtagttg tcccggacag tccgctgaat gcggaatccc cgatcgtcgt gatactgata
     1921 tccccgatcg tgtcaggaat aaccagatcg gtatctgctc cggattgcgg ctcacagccg
     1981 gtaatgtccc cgttttcatc aaaatcaaat ttatatccgt ttaatgtcag cgtataatgc
     2041 agcacatcat cgctgtccag atacatatac gtattcccgt tatcact
//
LOCUS       DAOSYE010000403         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43887_length_2077_cov_3.106825, whole genome shotgun sequence.
ACCESSION   DAOSYE010000403 DAOSYE010000000
VERSION     DAOSYE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43887_length_2077_cov_3.106825"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLU43_07415"
     CDS             complement(<1..454)
                     /locus_tag="PLU43_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOO28278.1"
                     /translation="MEYETIISKMSLEDKVALGSGKDFWNTKDMVRYGIKGFTMADGP
                     HGVRRQQDQTDMLGINQAVPATCFPTAVLSACSWDEELLEQMGTAIAKEAKANGISVV
                     LGPGANIKRNPLCGRNFEYFSEDPYLTGKLAAAYIKGVEHEKISAGLKH"
     gene            complement(470..>2077)
                     /locus_tag="PLU43_07420"
     CDS             complement(470..>2077)
                     /locus_tag="PLU43_07420"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOO28279.1"
                     /translation="NFAGYLSFDIQAEEGQKLVLRFGELLDEYGELTQENIQCNKGKK
                     KTPLQQVVYRAKAGENKYKTRFAIFGFQYVEIDTDIALLAENFTGIAVYSGFTQTGRF
                     RSSNQLLNRFVQAAIWSAKSNSADLPTDCPTRERHGWTGDAQIFFESASYLFDYASFA
                     KKYLRDVYDWQKKSGKLPQIAPMGGVDFYMNVMNGSAGWSDIGILLPYHFWKLFGDEQ
                     ILEDYYDGMRAYAGFLMRRCGKWGGPYAKPLHLSGREKRFVVNTGQSYGEWAEPADVS
                     VLHWYDFARPHPEVSTAYTAYMMDLMEQIAVQLGKTEDIAGYHRYKEGCREAYRELVK
                     TKAYSLDTDRQARLVRPLAFGLLDKEQEKFAKKRLLQALENYGWRLGTGFLSTPLILD
                     VLMEIDPEAAYRLLENEEIPGWLSMPKAGATTIWESWEGPHAAAGVGSLNHYSKGAIC
                     RWLFASMCGIKPAGENAFLISPQPGGHVMFAEASYYSVYGMVSSGWERKEDGKLEYRI
                     SIPANTTAAIILPDGREYHYGAGNYQL"
BASE COUNT          479 a          602 c          417 g          579 t
ORIGIN      
        1 aatgcttcag tccagcactg attttttcat gctcgacacc cttgatataa gctgctgcca
       61 attttcctgt taaataagga tcttccgaaa aatattcaaa attacggccg cacagcggat
      121 tccgcttaat gttcgcgccc ggtccgagaa ctacactgat cccgtttgcc tttgcttctt
      181 ttgcaatggc tgttcccatc tgttcaagca gttcttcgtc ccagctgcag gcggagagaa
      241 ccgctgtcgg aaaacaggtc gccggcacag cctgattaat gccaagcata tcggtctggt
      301 cctgctgcct gcggacacca tgaggaccgt ccgccattgt aaagcctttt attccatacc
      361 gcaccatgtc ctttgtattc cagaaatctt ttccgcttcc gagggcaacc ttgtcctcca
      421 ggctcatttt actgatgatc gtttcatatt ccatgtgata gctgcctttc tataactgat
      481 agtttcccgc accataatga tattctctgc cgtccggcag aataattgct gccgttgtat
      541 tggccgggat cgaaatgcgg tattccagct ttccgtcttc ttttctctcc catccgcttg
      601 aaaccattcc atatacacta taataggaag cttcggcaaa cataacatgt ccgcccggct
      661 gcggcgaaat aagaaacgca ttttcccctg ccggcttgat cccgcacatg gaggcaaaca
      721 gccacctgca gatcgcccct ttggaataat gattgagcga gcctactccc gcggctgcat
      781 gcggaccttc ccacgattcc cagatcgttg tcgccccggc cttcggcatt gacagccagc
      841 ctgggatctc ttcgttttcc agcagacggt aggctgcttc cggatcgatc tccatcagca
      901 catccagaat caacggtgtg gataaaaagc cggtccccag tctccacccg taattctcaa
      961 gcgcctgcag cagacgtttt ttcgcaaatt tctcctgctc tttgtctaac agtccaaatg
     1021 ccagaggacg cacaagacgg gcctgtctgt ctgtgtcaag cgaataggct tttgtcttca
     1081 caagctccct gtaagcttcc cgacatcctt ccttatatct atggtatccg gcaatatctt
     1141 ctgtttttcc aagctgtacg gcgatctgtt ccatcagatc catcatataa gccgtatacg
     1201 cggtcgacac ttccggatgc ggcctggcaa aatcatacca gtgaagcaca ctgacatcag
     1261 ccggttccgc ccattctccg tatgactgcc cggtatttac aacaaagcgt ttttctctgc
     1321 cggaaagatg cagcggcttt gcataaggac cgccccattt tccgcaccgt ctcatcaaaa
     1381 atccggcata cgccctcatg ccgtcataat agtcttccag tatctgttca tcgccgaaca
     1441 gtttccaaaa gtgatatgga agcaggatcc caatatcaga ccatcctgca cttccgttca
     1501 ttacattcat ataaaaatcg acgccgccca ttggcgcgat ctgcggcagt ttgccgcttt
     1561 tcttctgcca gtcatataca tcccgcaaat atttttttgc aaaagaggca taatcgaaca
     1621 ggtacgaagc cgactcaaaa aatatctgcg catctccggt ccagccatgc cgctcccttg
     1681 tcggacagtc tgtcggcaga tccgcgctgt tgctttttgc agaccatatt gcagcctgta
     1741 cgaacctgtt cagcagctga ttggagcttc gaaaccttcc tgtctgtgta aatccggaat
     1801 ataccgcgat ccctgtaaaa ttttccgcta agagcgcaat atccgtatct atctctacat
     1861 attgaaaacc gaatatcgca aaccttgtct tatatttatt ttctcccgct ttcgcacgat
     1921 aaacgacctg ctgcagcggg gtctttttct tccctttatt gcactggata ttttcctgcg
     1981 tcagttctcc gtattcatca agcagttctc caaaacgcag cactaatttt tgtccttcct
     2041 ctgcctggat gtcaaaggaa agatagcctg caaaatt
//
LOCUS       DAOSYE010000404         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43899_length_2077_cov_2.721068, whole genome shotgun sequence.
ACCESSION   DAOSYE010000404 DAOSYE010000000
VERSION     DAOSYE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43899_length_2077_cov_2.721068"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2077
                     /locus_tag="PLU43_07425"
     CDS             <1..>2077
                     /locus_tag="PLU43_07425"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOO28280.1"
                     /translation="MTGLFICAEYLFEKKEERTHYFLFRLGAGTLASAAVSAFIVIPQ
                     LLQTFVSARFENENSGGLLMQYLAILRQVTGAYTTRWWSLLGLSFAAALIVCGLVKFR
                     KEKKTVFLVCSAVLMMVLELFFESVNLMWHFGSYIQYPIRNGFMIYTVIASCACYYAG
                     RLFPKEERGLFCGKKKQILKAGAGLAVTAILAGIGIMLYRAHPGLQLRIVFHTTSIVM
                     AAAFFLYLSFFVWKSGKYKYYSVFLLMAELIFYTFLLLGKPSFITGYSEEPEQGGEYI
                     RICNQLAGDFSIEPDYLARVKNPDESLNANYGLILRRPALSNWTHLISPELQQGAGAW
                     GYSWQFTRLLDAGGTAFSDALLGVTETISCLVQDPALYEKKASAEVVVDHTSGRQDTY
                     TLYQNQYTLPWGLTAAQADMVSKWMGTDLADTDADIVSLQNSLYRALAKESGSGEIAC
                     WIKNGDDLNQNSAVSDEEKTDGLVNTETVIIQVQDMTALYFQGGGADREDKNTEIFVN
                     GEPAAVPSIKETDNTLYPAHFNNNAICLGTFENEQVTVEIKTDLSVTDKSETQISALD
                     IGKLGELCKSYQNYDTKVSAGKHSLSAQIYAEEDGEFLLLPLAYDDGWNVRVNQKTVQ
                     ASSYAGIFTAIPLEKGDNEVTMTYLPKGMKQGLLLTAGTLLALFTAMLFRLKRPGVRK
                     PEGSFMEPVY"
BASE COUNT          557 a          423 c          556 g          541 t
ORIGIN      
        1 tatgacaggg cttttcatct gtgccgaata tctttttgag aagaaggaag agagaacgca
       61 ttattttctt ttccggcttg gtgccggaac gctggcatcc gcagcagtct ccgcatttat
      121 tgtcataccg cagcttttgc agacgtttgt ctccgcaagg tttgaaaatg agaacagcgg
      181 cggtttgctt atgcagtatc ttgcgatctt aagacaggta accggcgctt atacgaccag
      241 atggtggtcg ctgctcggtc tgtcgtttgc agcagcactg attgtctgcg ggcttgtaaa
      301 gttcagaaaa gaaaagaaaa cggtttttct tgtatgcagt gcagttttga tgatggtact
      361 ggaactgttt tttgaaagtg taaacctgat gtggcatttc ggttcctata ttcagtatcc
      421 gatccgcaat ggttttatga tttatactgt gatcgcgtcc tgtgcgtgtt attatgcggg
      481 cagattgttt ccaaaagagg aaaggggtct gttttgcgga aagaaaaagc agatccttaa
      541 ggcaggggcc ggtcttgccg ttacggctat ccttgccggt attgggatca tgctgtaccg
      601 ggcacatccg ggactgcagc ttcgaatagt atttcatacg acttccatcg ttatggcagc
      661 cgcctttttt ctgtatctta gtttttttgt atggaaaagc gggaaatata aatattacag
      721 cgtattttta ctgatggcgg aactgatttt ctataccttt ctgctgctcg ggaaaccgtc
      781 gtttataaca ggttattccg aagaaccgga acagggggga gaatatatca gaatctgcaa
      841 tcagcttgca ggagattttt caatagaacc ggattatctt gccagagtga aaaatccgga
      901 tgagagcctg aatgcaaatt acggactaat tctgcgccgg cctgcacttt ccaactggac
      961 acacctgatt tcgccggagt tgcagcaggg tgccggtgca tggggataca gctggcagtt
     1021 tacccggctt ttggatgcag gagggaccgc attttccgac gcgctgcttg gagtcacgga
     1081 gacgatcagc tgtctcgtgc aggatccggc actgtatgaa aaaaaagcaa gtgcagaagt
     1141 cgttgtcgat catacgtcag gccggcagga tacgtatacg ttatatcaga atcaatatac
     1201 actgccgtgg ggacttacgg cagcgcaggc agatatggtt tctaagtgga tgggaaccga
     1261 tcttgcggac acggatgcgg atatcgtttc cctgcagaat tcgctgtatc gggcattggc
     1321 gaaagaatcc ggctcaggtg agatcgcctg ctggataaaa aacggggacg atctgaacca
     1381 gaacagcgca gtaagcgatg aagagaaaac agatggtctg gtaaataccg aaacggttat
     1441 catacaggta caggatatga cggcacttta ttttcagggc ggcggcgcag accgggaaga
     1501 caaaaatacg gagattttcg taaatggaga accggcggca gtcccaagca tcaaggaaac
     1561 cgacaataca ttatatccgg cccattttaa taacaatgcg atctgccttg gtacatttga
     1621 aaatgaacag gtaacggtag agatcaaaac agatctgtcc gtcacggata aatctgaaac
     1681 gcagatcagt gcgcttgaca tcggaaagtt aggagaattg tgtaaatcct atcaaaatta
     1741 tgacacaaag gtgagtgctg gaaaacattc gctttctgca cagatatatg cggaagaaga
     1801 cggagaattt ctgctgctcc cgcttgctta tgatgacgga tggaatgtac gggtcaacca
     1861 aaaaaccgtg caggcaagct cctatgcagg aatttttacg gccattcctc ttgaaaaggg
     1921 cgataatgag gtgacgatga cctatctgcc gaagggaatg aaacagggac tgctgcttac
     1981 ggcaggtacg ctgcttgcgc tgtttacagc catgcttttt cgtttaaaga gaccaggggt
     2041 caggaaaccg gaaggcagtt ttatggaacc tgtatat
//
LOCUS       DAOSYE010000405         2074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_43987_length_2074_cov_2.796434, whole genome shotgun sequence.
ACCESSION   DAOSYE010000405 DAOSYE010000000
VERSION     DAOSYE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43987_length_2074_cov_2.796434"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLU43_07430"
     CDS             <1..293
                     /locus_tag="PLU43_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO28281.1"
                     /translation="LGRLIPRFKIRTKKRVIQTIVNKMEENAADGLNYSGKCYISESA
                     CMEDARAVADLVETRFPKLNGKVEIYSIGTTIGSHTGPGTVALFFWGSRRTD"
     gene            319..1188
                     /locus_tag="PLU43_07435"
     CDS             319..1188
                     /locus_tag="PLU43_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO28282.1"
                     /translation="MKTAIMTDGNSGITVAEAEQSGIFRMRMPVIIDGENYFEGINLT
                     QDIFYGSLSAGKDVTTSQPSPGDVIEMWEDIFSKGYDEIVYIPMSSGLSNSCESAQGL
                     AKEYDGHVQVADNHRISVTQRQSVFDAKQMADAGSPAVDIRKYLEQTAYDSSIYIAVN
                     TLEFLKKSGRVTAAGAALGTMLNIKPLLTIQGGKLDAFAKVRGMKKCETKIIEAVHHD
                     ITVRFAGVKKSGLIIGTAGTFLKDEDAQGWCDSVRDAFDGIEVYYNQLSLSIGSHVGP
                     DAIGIGVSVKVSA"
     gene            1228..1890
                     /locus_tag="PLU43_07440"
     CDS             1228..1890
                     /locus_tag="PLU43_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO28283.1"
                     /translation="MEREIDLAEISDGRLYNANDMVKAGCHDCAGCFACCRGMGTSII
                     LDPMDIYRMSDGLHRDFMQLLEESLELTVVEGIIQPNIRMQGENEQCVYLSENGRCGI
                     HEFRPGFCRMFPLGRIYENGRFRYFLQVHECVKQVRTKVKVRKWIGIPNIEKYECYIT
                     DWHYFLKHVQEWFCDMEDEQIKKYNLYLLHEFYKKPYEAADFYGQFYERMQNAKEAVT
                     SV"
BASE COUNT          619 a          333 c          566 g          556 t
ORIGIN      
        1 atttagggag actgattccg agatttaaaa tacgtacaaa gaagagagtc attcagacga
       61 ttgtaaataa aatggaagag aatgctgctg atgggcttaa ttattccggg aaatgttata
      121 tttccgagtc agcctgtatg gaagatgcaa gggcagttgc agatcttgta gagacgcgtt
      181 ttccaaagct taacggcaag gtggagattt attcgatcgg gacgacgatc ggcagtcata
      241 ctggtccggg tacggtagca ctgttctttt ggggcagcag acgtactgac tagtcagcgg
      301 agggagaagt aaggattcat gaaaacggca attatgacag atggcaacag cgggattact
      361 gttgcggagg cagaacagtc cggtattttc aggatgcgga tgcctgtaat tatagacggg
      421 gaaaattatt ttgaaggaat caacctgaca caggatatat tttacggcag tctttcagct
      481 ggtaaggatg tgacgacttc ccagccttca ccgggcgatg tgatagaaat gtgggaagat
      541 attttttcaa aagggtatga tgaaattgtt tatattccga tgtcgagcgg tctgagtaat
      601 tcctgtgaaa gtgcacaggg acttgcaaag gaatatgacg gacatgtgca ggtggcagac
      661 aaccacagaa tatccgtgac ccagcgccag tccgtatttg atgcaaaaca aatggcagat
      721 gcgggcagcc cggcagttga catcaggaaa taccttgaac agacagcgta tgattccagc
      781 atttatattg cagtcaatac gcttgaattt ttaaaaaaga gcggcagggt gacagcggca
      841 ggtgctgcgc ttggaacgat gctgaatatt aagccgctgc ttacgatcca gggcgggaag
      901 ctggatgcgt ttgcgaaagt acgcggtatg aaaaaatgtg agacaaaaat cattgaagct
      961 gttcatcatg atataactgt tcggtttgca ggtgtaaaaa agtccggact gattattggt
     1021 acggcaggta cgtttttaaa ggacgaggat gcacagggct ggtgcgattc ggtaagagat
     1081 gcgtttgatg gaatagaggt ttattataat cagttatctt taagtattgg cagtcatgtt
     1141 ggtccggatg cgatcggtat tggtgtgagt gtaaaggtat cggcataata cgtttacttt
     1201 gcagatattt tcagacagga tgattttatg gaaagagaga tagatttagc tgaaatttca
     1261 gatggaaggt tatataatgc gaatgatatg gtaaaggcag gctgtcatga ttgtgcaggc
     1321 tgctttgcct gttgcagggg aatgggaaca tcgattattc ttgatccgat ggatatttat
     1381 cgtatgtcgg acggacttca cagagatttt atgcagctgc tggaggaaag ccttgagctt
     1441 actgttgtag agggaattat tcagccgaat atccggatgc agggagaaaa tgaacagtgt
     1501 gtttatctca gcgaaaacgg gcgatgcggg attcatgaat tccgcccggg cttctgtcgg
     1561 atgtttccgc ttggaaggat ttatgagaac ggccgcttcc gttacttttt acaggtacat
     1621 gaatgtgtaa aacaggtcag aacaaaagta aaggtacgta aatggatcgg gatccctaat
     1681 attgaaaaat atgaatgcta tatcacggac tggcattatt ttttaaaaca tgtgcaggag
     1741 tggttttgtg acatggaaga tgagcagata aaaaaatata atctgtatct gctgcatgaa
     1801 ttttataaaa aaccttatga agctgcagat ttttacggac agttttatga aagaatgcag
     1861 aatgcaaaag aagccgtcac ttcagtttga atgacggctt cttatcttat ggcaaataag
     1921 caagatactg tgtgaaccca acgatcaatg tgatcgtgat gatagaaaac agtgtggaaa
     1981 cggctaccgt accggcggat agtttggcat ccgcatgaaa tttaatggaa aacatcgttg
     2041 aagaagccgc tacgggtgcg ctggcagata taat
//
LOCUS       DAOSYE010000406         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44026_length_2073_cov_3.139247, whole genome shotgun sequence.
ACCESSION   DAOSYE010000406 DAOSYE010000000
VERSION     DAOSYE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44026_length_2073_cov_3.139247"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLU43_07445"
     CDS             complement(<1..724)
                     /locus_tag="PLU43_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOO28284.1"
                     /translation="MKNLKVALLQLLPDDTIDGNLEKGLKYCSTAKQMGADIALFPEM
                     WSVGYNIPEDIDELKAKALSADSAFVDSFGKCAKKLDMAIGITFLEKYDPLPRNVLCL
                     FDRFGNKVFTYAKVHTCDFGEESGLTAGDDFYAADLNTKQGNIKVGAMICYDREFPES
                     ARILMLKGAEIILVPNACPMEINRISQLRARAFENMVGIATVNYPEGKVDCNGHSTAF
                     DGIAYKPSKSGSRDTLILEAGEQ"
     gene            complement(831..903)
                     /locus_tag="PLU43_07450"
     tRNA            complement(831..903)
                     /locus_tag="PLU43_07450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(868..870),aa:Ala,seq:ggc)
     gene            complement(936..1880)
                     /locus_tag="PLU43_07455"
     CDS             complement(936..1880)
                     /locus_tag="PLU43_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOO28285.1"
                     /translation="MREEIIYELKSIYREPMHVRAFRFGSGKDSVCIMGATRGNEMQP
                     LYICSRLIRALREEERRHHFVKDRSVMVIPTINSYSMNVGKRFWPTDNTDINRMFPGY
                     AEGETTQRIAAGIFERISRFEYGIQLASYYMPGYFIPHVRIMKTGYEEPDLARLFGLP
                     YVMLRQPKPYNTATLNYNWQIWNTPAFSVYTCTADQIDEESADIGVKAVLNFLRAAGI
                     LRGEAEQGSDSRVVNYEALIAVSTSKAGLFRCLVDLNQEVHKGDVIASVLDPYTGEEL
                     ERILAPADARVFFMHNQPLVYAYSVIFKLIPLDIPIKK"
     gene            complement(1877..>2073)
                     /locus_tag="PLU43_07460"
     CDS             complement(1877..>2073)
                     /locus_tag="PLU43_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOO28286.1"
                     /translation="IFIPAVEHWAGIEKGDHIGDIIHPLYGTCVQRVTSPGSGLIFTL
                     REHPVVDEGSLIARILEKET"
BASE COUNT          556 a          536 c          413 g          568 t
ORIGIN      
        1 cttgctcacc ggcttccaga ataagagtat ctcttgaacc agatttagaa ggtttgtatg
       61 cgatcccgtc aaacgccgtc gaatgaccat tgcagtcaac tttgccttcc gggtagttga
      121 cagttgcaat gccaaccata ttttcaaatg ccctcgcccg cagctgtgat atccggttta
      181 tttccatagg gcaggcattt ggtactaaaa taatctccgc accttttaac ataaggattc
      241 ttgcactttc cggaaattcc ctgtcataac agatcattgc gccaactttt atattaccct
      301 gcttcgtgtt caaatcggca gcataaaaat catcacctgc cgtcagtccg gattcttctc
      361 caaaatcaca ggtatgcact tttgcatatg taaatacctt gttgccaaac cgatcgaaca
      421 gacaaaggac gtttcttggc aatgggtcat acttctcaag aaaagttatc ccaatagcca
      481 tatccaactt ctttgcgcat ttgccaaacg aatcaacaaa ggcgctgtca gctgataatg
      541 cctttgcttt caattcatca atatcttccg ggatattata accaacactc cacatttcag
      601 gaaacagagc aatgtcggcg cccatttgtt ttgctgtgct gcaatatttt aatccttttt
      661 ccaaatttcc gtctatagtg tcatcaggca gcagctgtaa caaagccact ttcagatttt
      721 tcattgtgaa aatcctcctt cctagtttct atcattcatt ttatcacatc tgcacagcag
      781 atggttcaat aaaaaaaaac catgtctttt gacatgagcc ttttttctga tggagatagt
      841 gggattcgaa cccatgacct cttgaatgcc attcaagcgc tctcccagct gagctatacc
      901 cccggaatat aaaaatcgaa cataaaaata ctttattatt tttttattgg aatgtcaagc
      961 ggtatcagct taaagattac cgagtaagca tacaccagcg gctgattatg cataaaaaat
     1021 accctggcat cggcaggggc taaaatgcgt tccagttctt cccctgtata tggatccaga
     1081 acagaggcaa tcacatctcc tttgtgtact tcctgattca gatctaccag acacctgaac
     1141 agcccggcct ttgaggtact taccgcaatg agtgcctcat agttaacgac cctggaatca
     1201 gaaccttgtt cagcctctcc gcgaagaatt ccggcagcac gcagaaaatt cagtacggct
     1261 ttcactccga tgtcggcact ttcttcatcg atctgatctg ctgtgcatgt ataaacggaa
     1321 aatgccggcg tattccagat ctgccagttg taattaaggg tagcggtatt gtaaggcttc
     1381 ggctgacgga gcatcacata gggcagaccg aacagacgcg ccagatcagg ctcctcatat
     1441 cctgtcttca tgatcctcac atgcggaata aaataacccg gcatataata gcttgccaac
     1501 tggatcccgt attcgaaccg gctgatccgc tcaaaaatac cggcagcgat ccgctgcgta
     1561 gtttcccctt cggcgtatcc cggaaacatg cggttgatgt ccgtattgtc ggtcggccag
     1621 aaccgttttc caacattcat cgaatagctg ttgatcgtcg gaatgaccat aacagaccgg
     1681 tctttgacaa aatggtgcct tcgctcctct tctctaagcg cccggatcag acgggaacag
     1741 atataaagcg gctgcatttc attgccgcgg gtagctccca taatgcagac ggaatccttt
     1801 ccgctgccaa atcggaaagc acggacatgc ataggctccc ggtaaataga tttcaattca
     1861 taaataattt cttctctcat gtttccttct ccagaatgcg ggcgatcagg gacccttcgt
     1921 ccacgaccgg atgttcccgc agcgtaaaaa taagcccgct gccgggcgac gtgactcgct
     1981 ggacacaggt tccgtaaaga ggatggatga tatctccgat atgatcaccc ttttcgatcc
     2041 ctgcccaatg ttccacagca ggaataaaaa ttc
//
LOCUS       DAOSYE010000407         2065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44345_length_2065_cov_2.609950, whole genome shotgun sequence.
ACCESSION   DAOSYE010000407 DAOSYE010000000
VERSION     DAOSYE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44345_length_2065_cov_2.609950"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2065
                     /gene="feoB"
                     /locus_tag="PLU43_07465"
     CDS             <1..>2065
                     /gene="feoB"
                     /locus_tag="PLU43_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOO28287.1"
                     /translation="AGNPNSGKTTLFNALTGANQFVGNWPGVTVEKKEGKLKNHKEVV
                     IMDLPGIYSLSPYTLEEVVARNYLITERPDAILNIVDGTNIERNLYLSTQLLELGIPL
                     VIAVNMMDVIKKTKDKIHIDQLSKKLGCEVVEISALKGDGVKEAAEKAVEAANRKKAA
                     VLVHKFAPEVESVIEETEYKLGAGIPREQRRFFAIKLLERDDKITEQMEQVPDVTEEI
                     KKLEEVMDDDTESIITNERYLYISSIIKDCYKKNERGRLTSSDKIDRVVTNRWLALPI
                     FAVVMFLVYYVSVTTIGTIVTDWTNDVLFGEIVPPAIEQFLAWIHCADWLQGLILDGI
                     VAGVGAVLGFVPQMFVLFIFLAFLEACGYMARVAFIMDRIFRKFGLSGKSFIPMLIGT
                     GCSVPGLMASRTIENDRDRKMTIITTSFIPCGAKLPIIALIAGALFHGAWWVAPSAYF
                     VGIAAVICSGVILKKTKMFSGEPAPFVMELPAYHMPTVGNVLRSMWERGWSFIKKAGT
                     IILLSTIVLWFLMSFGWADGSFGMLEAEQLDSSVLASVGSFIAPLFIPLGWGDWKMAV
                     AAVTGLIAKENVVGTFGVLFGFAEVAEDGSEIWGSLAGSISAVAAYSFLVFNLLCAPC
                     FAAMGAIKREMNSQKWFWFAIGYQTLLAYAVSLCVYQIGMLFTASSFGFFTIIALLIL
                     IGFVY"
BASE COUNT          520 a          407 c          578 g          560 t
ORIGIN      
        1 tggcaggtaa tccaaacagc ggaaagacca cgttatttaa tgcgctgacc ggtgccaacc
       61 agtttgtcgg caactggccc ggtgttacgg tagagaaaaa ggaaggaaag cttaaaaatc
      121 ataaggaagt cgttattatg gatcttcctg gtatttattc actgtctcct tatacactgg
      181 aagaggtcgt ggcaagaaat tatttgatta cggaacggcc ggatgccatt ctgaatattg
      241 tagacggaac aaatatcgag aggaacctgt atctttccac acagcttctg gaacttggaa
      301 ttccgcttgt gatcgcggtc aatatgatgg atgtgatcaa aaagacgaaa gacaagatcc
      361 atattgacca gctgagtaaa aagctgggct gtgaagttgt ggagatttca gcacttaagg
      421 gagacggtgt caaagaggcg gcggaaaaag cagtggaagc cgcaaacagg aaaaaagcag
      481 ctgtcctggt acataaattt gcgcctgaag tagaatcggt gattgaagag acggagtaca
      541 aactcggcgc tgggattccg cgtgagcaga gacgattttt tgcgatcaag ctgctggaaa
      601 gagatgataa gatcacggag cagatggaac aggtgccgga tgtgaccgaa gagatcaaaa
      661 aactggaaga ggtcatggat gatgatacgg aaagtattat cacaaatgaa aggtatcttt
      721 atatatcttc cattatcaaa gactgttata aaaagaacga aagaggcaga ctgacgtctt
      781 ccgacaagat cgatcgtgtt gtgacgaaca gatggcttgc tttgccgatc tttgctgttg
      841 tgatgtttct tgtgtattat gtgtctgtta caacgatcgg aacgatcgtg acagactgga
      901 caaatgatgt gctgttcggc gagatcgttc cgcctgcgat cgaacagttt cttgcatgga
      961 tccattgtgc agactggctg cagggcctga tcttagacgg tatcgtagcc ggtgtcggag
     1021 cagtactcgg ctttgtaccg cagatgtttg tactgtttat ttttctggcg tttcttgagg
     1081 cgtgcggata tatggccagg gtggcattca ttatggacag gatctttcgt aaattcggcc
     1141 tgtccggcaa gtcctttatt ccgatgctga tcggtacggg ctgcagtgtt ccgggtctga
     1201 tggcatccag aacgatcgaa aacgacagag accgtaaaat gacgatcata acaacatcct
     1261 ttatcccatg cggagccaag cttccgatca ttgccttgat tgcgggagcc ctgttccacg
     1321 gcgcatggtg ggtcgctccc agtgcatatt ttgttgggat cgcagcagta atctgttcgg
     1381 gagttatttt gaagaagaca aaaatgtttt caggggaacc ggcaccgttt gttatggagc
     1441 tgccggccta tcatatgccg acagtcggaa acgtactgcg cagtatgtgg gagcgcggct
     1501 ggtcctttat caagaaagcc ggaacgatta ttttactttc cacgatcgtg ctttggttct
     1561 taatgagctt tggctgggct gacgggtcgt ttggaatgct ggaagcagaa cagcttgaca
     1621 gcagtgtgct tgcttcggtc ggaagtttca ttgccccgct gtttataccg cttggctggg
     1681 gagactggaa gatggcggta gccgcggtga caggtctgat cgccaaggaa aatgtagttg
     1741 gtacgtttgg tgtcctcttt ggctttgcgg aggtggcaga ggacggatca gagatatggg
     1801 gctcgcttgc aggcagcata tcggctgttg cagcgtattc attccttgta ttcaacctgc
     1861 tttgtgcacc ctgcttcgca gcaatgggag cgatcaagag agagatgaac agtcagaaat
     1921 ggttctggtt tgcgatcgga tatcagacat tgcttgctta tgcggtatcc ctctgtgtat
     1981 atcagatcgg aatgctgttt acagcttctt cgtttggatt ttttacaatt atagcactgc
     2041 ttatcctgat cgggtttgtt tactt
//
LOCUS       DAOSYE010000408         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44412_length_2063_cov_2.752490, whole genome shotgun sequence.
ACCESSION   DAOSYE010000408 DAOSYE010000000
VERSION     DAOSYE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44412_length_2063_cov_2.752490"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLU43_07470"
     CDS             <1..811
                     /locus_tag="PLU43_07470"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOO28288.1"
                     /translation="RNANRIKDAKFSIDGVTYQLKVNDNANNLHSDQDIGYHKQLWDA
                     QIMDQSVKFSYFSPDGEMGFPGNLKISVTYTLTEGNALELSYYGVSDKKTVINLTNHT
                     YFNLSGHDAPDILDTEVTLQASHYTPIVPGAIPTGEIAAVAGTPMDFTKAKTIGKEID
                     TEWDQLTMVGGYDHNFAVDHYDGSLQKIAEVSADNRTMEVYTDLPGVQFYTGNGTQKM
                     AGKGGALYGPRTAFCLETQYFPNSINQEGFIKPVFDAGEVYQTKTVYKFVR"
     gene            842..1177
                     /locus_tag="PLU43_07475"
     CDS             842..1177
                     /locus_tag="PLU43_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28289.1"
                     /translation="MKKKRNRVPTQMNYLRIVVGGYLVYLAYDIFRLRDTQASDYWLM
                     MLFVLLFTIFGGVIVIFSLKHLIKKEYYDPNNMDIEDEPEEQTPKIEEDSMEEEQTGG
                     ESESEKNNL"
     gene            1318..>2063
                     /locus_tag="PLU43_07480"
     CDS             1318..>2063
                     /locus_tag="PLU43_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28290.1"
                     /translation="MSNKNDEPDEKKKSKRKSHATMKRQKDQVSVTLAGMAIYSVVLI
                     AIVAGTYLGIKAIFSGYQKNTAVTETTETADQTETPEEETVSGSESEVLTEPASDAET
                     DDDETMQEELIDISSLVSGDNHYIDYSQTLFTPAERDTSLTWDDSVFLKLENVKNPSE
                     SLVNTYQFSRKFATREDDKKMEFDVYTNPDTKEIEKITAIEYCGDDTEAIDYYFDHGK
                     INYAAQRRSVINTPVDISSAAVQSRYYFNQ"
BASE COUNT          674 a          378 c          522 g          489 t
ORIGIN      
        1 aagaaatgca aacaggatca aagatgcaaa attttcaatc gatggtgtaa cgtaccagct
       61 gaaagtcaat gacaatgcaa ataatctgca cagtgatcag gatatcggat atcataaaca
      121 gctttgggat gcacagatca tggatcagtc ggtaaaattt tcttattttt ccccggatgg
      181 agaaatgggc tttccgggaa atctgaaaat ttccgtcacc tatacgctga cggagggaaa
      241 tgcactggaa ttgtcttatt atggtgtcag cgacaaaaag acagtgatca atctgacgaa
      301 tcatacatat ttcaatcttt ccggtcatga tgcaccggat attctggata cggaagtaac
      361 gctgcaggca tctcactata caccgatcgt gccaggggcg atcccgacag gggagatcgc
      421 ggctgttgcc ggtacgccga tggattttac aaaggccaaa acaatcggga aagaaataga
      481 cactgagtgg gatcagctta cgatggtagg cgggtatgat cataattttg cggtggatca
      541 ttatgacggt tcacttcaaa aaatagcaga ggtatccgca gacaacagga caatggaagt
      601 ttatacggat ctgccgggcg tgcagtttta tacggggaac ggaacgcaga aaatggccgg
      661 caaaggcggt gccttatacg gaccgagaac cgcgttctgt ctggaaacgc agtattttcc
      721 aaacagcata aatcaggaag gattcatcaa gccggtattt gatgccggcg aagtctatca
      781 gacaaagacc gtatataagt ttgtccggta atatgcggat caaaaaggaa ggaggatgaa
      841 catgaaaaaa aagaggaaca gagtgcctac ccagatgaat tatttaagaa tcgtagtcgg
      901 cggatacctc gtttatcttg cttatgatat tttcagactc agggatacgc aggcatctga
      961 ttattggctc atgatgctgt ttgtgctgct gtttacgatt ttcggcggag tgatcgttat
     1021 tttttcactg aagcatctga tcaaaaaaga atattacgat ccgaacaata tggatattga
     1081 ggacgagccg gaggagcaga cgccaaagat agaggaagac agcatggaag aggagcagac
     1141 cggcggggaa tcggaatctg aaaaaaacaa tttgtaaagc agctgagggg taaagggctg
     1201 ttggaacaca caaaggctat ttaataaaac ttaagaaatt ctttctgaat gttttctttt
     1261 cggatgttat gataaagaag agcagatgtg ttgtattcaa tggcaaggag agtatatatg
     1321 agtaataaaa atgatgagcc ggatgaaaag aaaaaaagca aacgcaaaag ccatgccaca
     1381 atgaaacggc aaaaggatca ggtttccgtg acgctggcag ggatggccat ttattcagtt
     1441 gtcttgattg cgatcgtggc cggaacatat cttgggatca aggcgatttt ttcaggatat
     1501 caaaaaaata cggcggtaac ggagacgacg gagacggcag accagacgga aacacccgaa
     1561 gaagaaaccg tttcgggaag cgaaagtgag gtcttgacag agccggcatc tgacgcagag
     1621 acagacgacg atgaaacgat gcaggaagag ctgatcgaca tctcctcgct tgtgagcgga
     1681 gacaaccatt atattgatta cagccagacc ctgtttacgc ctgcagagcg tgacacctcc
     1741 ctgacatggg acgacagtgt attcctaaaa cttgaaaatg taaaaaatcc ttcggaatca
     1801 cttgtaaata catatcagtt ttcacgaaag tttgcaacgc gtgaagatga taagaagatg
     1861 gaatttgacg tttatacaaa tccggataca aaagaaatcg aaaagattac ggcaattgaa
     1921 tattgcggag acgatacgga agcaattgat tattattttg atcatggaaa aataaattat
     1981 gcggctcagc ggcgttccgt aatcaacaca ccggtcgata tttcttcggc tgcggtacag
     2041 tccagatatt attttaatca gga
//
LOCUS       DAOSYE010000409         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44622_length_2057_cov_3.217283, whole genome shotgun sequence.
ACCESSION   DAOSYE010000409 DAOSYE010000000
VERSION     DAOSYE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44622_length_2057_cov_3.217283"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1925)
                     /locus_tag="PLU43_07485"
     CDS             complement(192..1925)
                     /locus_tag="PLU43_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO28291.1"
                     /translation="MMGKNESQKQNKKDGIVKKRSFFGSISFKIMLLVVLSMAIAATT
                     VVVILVKDAKKTLKDQVLSSMLYMAETQRTLLDNETGGVSATIAQYTAVLEGVKIDGV
                     DSSYAYLVSSDGIMKYHPTESKLGQPVENSVVKGLVAQIEAGETPEDAVVSYEYKGAM
                     KYAGYAITKNKSILVITADQSDVMSSINEITWVATGVYIAMILICAIIAFLISRMITK
                     PINQLTEVITTTADFDFKKSVYGESLVKRKDETGDMARAIRRMRANLREMVGSIDGAS
                     EKINGNVNQLQDVTNVVNHMCTDNSATTQQLAAGMEETAATAESIYANIGYMQTGAKD
                     IIQLSENGDAVSDEVMARANALMDKTMEATKRTKETYDSVKVRSSNAIEDSKAVSKIN
                     ELTEAIMAISSQTSLLALNASIEAARAGEAGRGFAVVATEIGNLAEQTSKAVTDINSI
                     VGEVNAAVGNMAGCLEETSSFLEKTVLSDYQEFTEVSEQYNGDARKFKESMKDVHESI
                     VNLAESISKISDALSGINATVGESTLGVTDIAGKTTDMVTRTSETNSLVEESLSCVEQ
                     LKTIVSTFKLD"
BASE COUNT          515 a          543 c          357 g          642 t
ORIGIN      
        1 tttttattca aatgaaaaac aaaaaaaact aaacatcctt ctaaaagcag cagtatcatc
       61 agcaagtata tcaaaatttt tctatttctc ataggacctc caaaacagca gcagccgcat
      121 tgttccatta gcaaaacaag gctgcagacc tttcgtcttg cagccttggc ttttatatat
      181 tatggatcgt cttaatccag tttaaacgta ctgactattg tcttcagctg ttccacacag
      241 ctaaggcttt cttctaccag actatttgtt tcgcttgtcc ttgtaaccat atccgtcgtc
      301 tttccggcaa tatccgtgac cccgagtgtc gattccccaa ctgtcgcatt gataccggaa
      361 agcgcatctg aaattttgga aatggactcc gccagattca cgatcgactc atgtacatct
      421 ttcatgctct ccttgaactt cctggcatct ccgttatact gctcacttac ttccgtaaac
      481 tcctgataat cggacaatac tgtcttttca aggaaggaac ttgtttcctc aaggcatcct
      541 gccatgttgc ctacggccgc attgacctct ccaacaatag aattgatatc cgtaacagcc
      601 ttggatgtct gctccgccag attaccgatc tcagtagcaa ctaccgcaaa tccccggcct
      661 gcttctccgg ctctcgctgc ctcaatactt gcattcagtg caagcagact tgtctgagac
      721 gaaattgcca tgatcgcttc tgtcagttca ttaatcttgg aaactgcctt ggaatcttcg
      781 attgcattgg aagatctgac ttttacggaa tcataggttt cctttgttct cttcgtagcc
      841 tccatcgtct tatccatcag ggcatttgcg cgggccatga cttcatcaga taccgcatcc
      901 ccattctccg aaagctgaat aatgtctttt gcgccagtct gcatatatcc gatatttgca
      961 taaatgcttt ctgctgtggc agccgtttct tccattcctg cggccagctg ctgagttgtt
     1021 gcggagttgt ccgtacacat atggtttacc acatttgtaa catcctgcag ctgattgaca
     1081 ttcccattga ttttttcact tgcaccgtca atactgccga ccatctcccg taaattcgca
     1141 cgcatccttc tgatcgcacg cgccatatct ccggtctcat ctttccgttt tacaagcgat
     1201 tccccatata cactcttttt aaagtcaaaa tctgccgttg tagtgatcac ctcggtcagc
     1261 tgattgatcg gctttgtgat catacggctg atcaaaaacg caataattgc acagatcagg
     1321 atcatagcga tatagacacc tgttgccacc cacgttatct cattgatcga agacattaca
     1381 tcactctggt ctgccgtaat aacaagaata cttttatttt tcgtgatcgc atagccggca
     1441 tacttcattg cccctttata ctcataacta acgaccgcat cctcaggtgt ttcgcctgct
     1501 tcgatctgcg caaccaggcc ttttacaaca ctgttttcaa caggctgacc aagtttggat
     1561 tcggttggat gatatttcat aataccgtcg cttgaaacaa gatatgcata agaagaatcc
     1621 acgccatcga tcttaactcc ctcaagcact gccgtatact gcgctatcgt agcactgact
     1681 cccccggttt cattatcaag cagcgtccgc tgtgtctctg ccatataaag catggacgac
     1741 aaaacctgat cctttagtgt cttctttgca tctttcacaa ggatcactac tactgtcgtt
     1801 gcggcaatcg ccatgctaag tacaacaagc agcatgatct taaagctgat tgaaccgaaa
     1861 aacgatcttt ttttaacaat tccatccttc ttgttttgtt tttgtgactc gtttttcccc
     1921 atcatatgtc ctttccggca caaaatgaac gtatgcacct atgcatctca attcggcttt
     1981 tcgccgaata ttttttttta catcagatag tatagtacaa ttttgtgaat atttcaacaa
     2041 aaaagcggca attatcg
//
LOCUS       DAOSYE010000410         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44713_length_2054_cov_4.359180, whole genome shotgun sequence.
ACCESSION   DAOSYE010000410 DAOSYE010000000
VERSION     DAOSYE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44713_length_2054_cov_4.359180"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /locus_tag="PLU43_07490"
     CDS             complement(<1..2051)
                     /locus_tag="PLU43_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOO28292.1"
                     /translation="MAEFDGARDELVKNTGIIELSGCADSQKLHMFYGFSDGFQYKII
                     VTFHEQKAKEICEDYQFYDKQVCFYPAKDLIFYQADIRGNLLTKQRLKCLKALFLNEP
                     VTIVTTFDAFMDHIVPLSVIRDHIIELMVGDTLDLKDFVRELTALGYERNYQVESEGQ
                     FSLRGGIVDIFPLTEENPIRIELWGDEIDSVRSFDILSQRSLEKMDTVTIFPATETVL
                     TEEEKQDGLRRLEKDAGKFAERLRKDFKTEEAHRIKELVAELKEKLLELGTGGCNIES
                     CLDYFYESHVSLFDYFDKEKTVFFLDEPARLYEKGSVVEQEFRESMEQRLAKGYVLPG
                     QTDILYAKEEIFGRLEASHCISLSALQVRKGICRTTAKHSIAAKAMNSYNNSFEALVS
                     DLRKYKKNGYRVILLSASRTRAVRLSSDIREQELSSFFTEDYDHTVKPGEIMVAYGRI
                     RQGFEYPMLKFVVISESDIFTAQKKKRPKRKKYEGQKIQNFSELSVGDFVVHESHGLG
                     IYKGIEQVTVDKVTKDYMKIEYRDGGNLYIPATALDAIQKYASKSSSRPRLNKLGGTE
                     WTKTKAKVKSAVDVIASDLVELYAVRREKSGYQFSRDTVWQTEFEELFPFEETEDQLL
                     AIDATKQDMESSKIMDRLICGDVGYGKTEIAIRAAFKAVQDGKQVVYLVPTTILAQQH
                     Y"
BASE COUNT          511 a          571 c          357 g          615 t
ORIGIN      
        1 ttataatgct gctgtgccag aatcgtcgtc ggcaccagat aaacgacctg cttcccgtcc
       61 tgtacggcct tgaatgccgc cctgatggca atctccgtct ttccgtatcc gacatccccg
      121 cagatcagcc ggtccatgat cttactgctt tccatatcct gctttgtcgc atcaatggcc
      181 agaagctgat cctctgtctc ctcaaacgga aacagctcct cgaactctgt ctgccatacg
      241 gtgtccctgc taaactgata accgctcttc tctctgcgca ctgcataaag ctccacaaga
      301 tccgacgcaa tcacatcgac cgcactcttt acttttgctt ttgtctttgt ccattcggta
      361 ccgcccagct tattcagcct tggccgggat gaagatttgg aggcatattt ctgaatggcg
      421 tccagtgctg tcgccggaat atacagattg ccgccgtcac ggtattcaat cttcatataa
      481 tcctttgtta ctttgtcgac cgtcacctgc tcgattcctt tatagatacc aaggccatga
      541 ctttcatgaa cgacaaaatc tccaacagac agttcggaaa aattctgtat tttctgtcct
      601 tcatattttt ttcttttcgg ccgttttttc ttttgcgccg taaaaatatc actttccgaa
      661 atcacaacaa atttcagcat cggatattca aatccctgcc ggatacgccc atatgccacc
      721 atgatttctc ccggcttcac ggtatggtca taatcctccg taaaaaaact gctcaattcc
      781 tgttccctga tatctgaaga aagccttacg gcccttgttc ttgatgctga taaaaggatg
      841 acacgatacc cgttcttttt atattttcgc agatccgaaa caagtgcctc aaaactgtta
      901 ttataagaat tcatcgcttt tgcggcgatc gaatgctttg ctgtcgttct gcagatccct
      961 tttctgacct gcagggccga cagggaaata caatggcttg cctccagacg tccgaagatc
     1021 tcttcctttg catacaggat atctgtctgt cccggcagca catacccctt tgcaagacgc
     1081 tgttccatgc tttcccgaaa ttcctgttcc acaacgcttc ctttttcata cagtctcgcc
     1141 ggctcatcca aaaaaaacac tgttttttct ttatcaaaat aatcaaacag ggacacatga
     1201 ctttcataaa aatagtccag acagctctct atattgcatc cgcctgttcc aagctccagc
     1261 agcttttctt tcagttctgc cacaagttct ttgattctgt gtgcttcttc ggtcttaaaa
     1321 tcctttcgaa gtctttccgc aaattttccc gcgtcttttt cgagccgtcg aagtccatcc
     1381 tgcttctctt cctcggtcag gaccgtttcc gtggccggaa atatcgtaac agtatccatt
     1441 ttttccaaag aacgctggct taaaatatca aagcttctga cagaatcgat ctcgtccccc
     1501 cacagctcga ttctgatcgg attttcctct gtcagcggaa aaatatcaac aatccctccc
     1561 cgcagggaaa actgtccctc agactctacc tgataattac gttcatagcc aagtgccgtc
     1621 agctcccgga caaaatcttt caaatcgagg gtatcaccaa ccatcagttc tattatatga
     1681 tccctgatta ccgacaacgg cacaatatga tccataaaag cgtcaaacgt tgtcacgatc
     1741 gttaccggct cgttcagaaa aagagccttc aggcatttta aacgctgctt tgtcaacagg
     1801 tttccgcgaa tatccgcctg ataaaaaatc agatccttgg caggataaaa acatacctgt
     1861 ttatcgtaga actgataatc ctcacatatt tcctttgctt tctgttcgtg aaaagtaacg
     1921 atgattttat actgaaaacc atcgctgaag ccataaaaca tatgcagttt ctgagagtct
     1981 gcacaaccgc ttagttcaat aatacccgta ttttttacga gctcatctct cgccccatca
     2041 aattctgcca gctc
//
LOCUS       DAOSYE010000411         2052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44820_length_2052_cov_1.774662, whole genome shotgun sequence.
ACCESSION   DAOSYE010000411 DAOSYE010000000
VERSION     DAOSYE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44820_length_2052_cov_1.774662"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLU43_07495"
     CDS             <1..1021
                     /locus_tag="PLU43_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO28293.1"
                     /translation="GRPGTVGNRSGNFITQNADLVLVLGCRLNIRQISYHWEMFARSA
                     FKIIVDIDKNELKKPTLTPDLPVNANVADFIKAMLRKSEKIELKKEWMDYCRTIRNKF
                     TTRSTLFPERKAKVNPYYFVESLTEKLKENDIIVTANGSACVVTFQVAFIKKGQRLYT
                     NSGCASMGYDLPAAIGAYTASSRKIYCVAGDGSIQMNIQELQTIFHDKMDITIFLFNN
                     DGYHSIRQTQNAFFGQPLVGVNSASGISFPDAEKIAEAYGLHFARIGKNISVNPVLDK
                     VLKMKGPVLCEVVLDPEQNFEPKLSSRNLGNGRMFTPPLEDMYPFLDRKTFKECMIIE
                     PIKEE"
     gene            1026..>2052
                     /locus_tag="PLU43_07500"
     CDS             1026..>2052
                     /locus_tag="PLU43_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HOO28294.1"
                     /translation="MKAIKILDCTLRDGGYVNDWNFGLGTIESIISRLDKAGIDIVEI
                     GFLDDRRKYDENRSILPDTDSIKPIFKNLDIQNAMILAMIDYGTCDIKALTHQKDSVL
                     DGIRVIFKKKDYPEALKFCAEIKKKGYKLSLQPVSVTTYSDSEMTELLKRVNEVAPYS
                     VSIVDTYGLMHKKELLHYFDLMDKNLTDGILIGYHSHNNFQLAYANCTELMAVESDRE
                     LMIDGSLYGMGKSAGNACTELLAMHMNENYGGRYNLDQLLEAIDVDIMKEYAKQYWGY
                     SFKHFIAASNDCHPDYVSNLLNKKTLSVRSINEIIAKIEPANKLTFNRDIMENLYLEY
                     QNNIIEDS"
BASE COUNT          664 a          438 c          446 g          504 t
ORIGIN      
        1 cggaaggccg ggaaccgtag gaaacaggag cggaaatttt attacacaaa acgccgatct
       61 ggttctggta ctcggatgca gactcaacat caggcagatc agctaccatt gggaaatgtt
      121 tgcaagatca gccttcaaga taatcgtgga tatcgataaa aatgagctga aaaaacctac
      181 ccttacccct gatcttcctg ttaacgccaa tgtcgccgac tttataaaag caatgctgag
      241 gaaatcagaa aaaatagagt taaaaaaaga gtggatggat tactgccgga caataagaaa
      301 taaatttacg acacgaagca cactcttccc ggaaagaaaa gcaaaagtaa atccctatta
      361 ctttgtagaa tccctgacag aaaaattaaa agaaaacgat attattgtaa cggcaaacgg
      421 cagtgcttgt gtcgtcacat ttcaagttgc ttttattaaa aagggccagc gactctatac
      481 caattcggga tgcgcgtcga tgggctatga tcttcctgcc gcgatcggag cctacacggc
      541 atccagcaga aaaatatact gcgttgccgg tgacggctcc atccagatga atatccagga
      601 actgcagacg atctttcatg ataaaatgga cataaccatt tttcttttta acaatgacgg
      661 ataccactcc atcaggcaga cgcaaaatgc tttttttgga caaccgcttg tcggtgtcaa
      721 ttccgcttcc ggcatcagct ttcccgatgc ggaaaaaatt gcagaggcct atggactgca
      781 ttttgccaga atcggaaaaa atatctcagt caatccggtc ctggataaag tattaaaaat
      841 gaaaggacct gttttgtgcg aggtcgtcct ggatcccgaa cagaatttcg agccgaagct
      901 gtcctcccgc aatctcggca atggcaggat gtttacgccg ccactggaag acatgtaccc
      961 ttttctcgac agaaaaacat ttaaagaatg catgatcata gaaccgatca aagaggaata
     1021 ataatatgaa ggctataaaa atccttgatt gcactttgcg cgacggcggc tatgtcaatg
     1081 actggaattt cgggcttggc acgatagaaa gcattatcag ccggctggac aaagcgggaa
     1141 tcgatatcgt ggaaatcggt tttctggatg acagaagaaa atatgatgaa aaccgttcta
     1201 ttctgcccga tacggattcc ataaaaccga ttttcaaaaa tctggacatt caaaatgcga
     1261 tgattctggc catgatcgat tacggcacat gcgatataaa agcgcttacc catcagaaag
     1321 attccgttct ggacggcatc cgggtcatct tcaagaaaaa agattatccg gaagcgctta
     1381 agttttgcgc agaaatcaag aaaaaaggat acaaattatc cttgcagcct gtttccgtaa
     1441 cgacttacag cgacagcgaa atgacagaac ttttgaaaag agtgaacgaa gtggcgcctt
     1501 acagcgtttc cattgtggat acctacggcc tgatgcacaa aaaggaatta ttgcattact
     1561 tcgatttaat ggacaaaaat ctgactgatg gaattttaat cggttaccat tcacacaaca
     1621 attttcagct tgcctatgcg aattgtactg aattgatggc ggtggaatct gacagagagc
     1681 ttatgattga cggatcgctc tacggcatgg gcaaaagcgc aggaaatgcc tgcacggaat
     1741 tactggccat gcatatgaat gagaactatg gtggccggta taatctcgat caactcctgg
     1801 aagccattga cgtcgatatc atgaaggagt acgccaaaca atactggggt tattcgttca
     1861 aacattttat cgccgcctcc aatgactgtc accccgatta cgtgagcaat ttactgaata
     1921 aaaaaacttt atccgtaaga tccatcaatg agattattgc gaaaatcgaa ccggccaaca
     1981 aactgacctt caacagggat attatggaaa atctctacct ggagtatcaa aataacatta
     2041 tcgaagattc cg
//
LOCUS       DAOSYE010000412         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_44913_length_2049_cov_2.857071, whole genome shotgun sequence.
ACCESSION   DAOSYE010000412 DAOSYE010000000
VERSION     DAOSYE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44913_length_2049_cov_2.857071"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..470
                     /locus_tag="PLU43_07505"
     CDS             45..470
                     /locus_tag="PLU43_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOO28295.1"
                     /translation="MNIKDQYRVLKFGEFGDERGNLVVAECGGMDVPFDVKRVFYMYG
                     SDTTVIRGRHANRRTKFVLINVCGSSKVKIDNGFESDTVELNKPRMGLYLPTMVWKDM
                     YDFSDDSILLVLASEHYDGAEYIRDYDEFLKEVGGMRHE"
     gene            463..1422
                     /locus_tag="PLU43_07510"
     CDS             463..1422
                     /locus_tag="PLU43_07510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28296.1"
                     /translation="MNKISIIIPVYYNSDTLLLLYEDMEKKILHKFSDYEIVFVDDGS
                     GDDSWDVMNRIRELDEHVKCVRLSRNFGEHAAILAGLSNCTGDCAVTKQADLQEDSEL
                     ILEMYESWMRGNKVVLAVRMQRDDSVIKQFFANMYYGIVRRFIEKKMPVGGFDCYLLD
                     RQVIGVLQMLDEKNSALTLQILWAGFRSEKIYFHRKDREIGKSRWTLSKKFKLVMDSM
                     MSFSYFPIRFMSGVGVICAIIAFLWIVEVILERLIIGTPIEGWASLMCLILFSTGMVL
                     LMLGILGEYLWRTLDASRNRPPFLVDTVNECRRNGTSESDEKI"
     gene            1438..>2049
                     /locus_tag="PLU43_07515"
     CDS             1438..>2049
                     /locus_tag="PLU43_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOO28297.1"
                     /translation="MEQKEKKEGILTGFIQYFYGNFVVLLLGFVSLPLITRVMTTDEY
                     GRTAMFTSAVSMIYIFAILGLDQSYIRYFYKEGVNRKKLFMQCLRFPVILIVLLAGLY
                     VLGARYFNGFLFGRSALDITLLVIAYTIISVFERFLFLNIRMEQNGKLYSNLNILSKV
                     LYILFILLFVAFLGNDFRVVLYAMTLSHTERPENHFPEMRQTAK"
BASE COUNT          539 a          355 c          550 g          605 t
ORIGIN      
        1 attgatacgg tcaatgcttt tcaataacag ataggagaag ccggatgaat ataaaggatc
       61 agtatcgggt gctgaaattt ggagaattcg gagacgaaag aggaaacctg gtcgttgcgg
      121 aatgcggcgg gatggatgta ccgtttgatg tgaaacgcgt attttatatg tatggttccg
      181 acacgactgt gatccgcgga cgccatgcga acagacggac aaagtttgta ttgatcaatg
      241 tgtgcggttc gagcaaagta aagatcgaca acgggtttga atcggatacg gtggagctga
      301 acaagccgag aatggggttg tatctgccta cgatggtatg gaaagacatg tatgattttt
      361 cagatgattc catacttttg gtactggcaa gcgaacacta tgacggagcg gaatatattc
      421 gtgactatga tgaatttttg aaagaagttg gaggcatgcg gcatgaataa gatttcgatt
      481 atcataccgg tctactataa ttccgatacg ctgctgctgc tttatgagga tatggagaaa
      541 aagatcctgc ataaattcag tgactatgag atcgtgtttg tggatgacgg ctccggggat
      601 gattcatggg atgtaatgaa ccggatacgt gaactggatg aacatgtaaa atgtgtacgt
      661 ttatccagaa attttggtga gcatgcagcc attcttgcag gcctgtcgaa ctgtacgggg
      721 gattgtgcgg tgacgaagca ggctgatctg caggaggatt ccgaactgat ccttgaaatg
      781 tatgagagct ggatgcgcgg aaacaaggtc gttcttgcgg tgcgcatgca gagagatgat
      841 tctgtgatca agcagttttt cgccaatatg tattacggga tcgtacgcag gttcatagaa
      901 aagaaaatgc ctgtgggcgg ctttgactgt tatcttctgg acagacaggt gatcggtgtt
      961 ctgcagatgc tggatgaaaa aaattcggcg ctcaccctgc agatcctatg ggcaggattt
     1021 cgttctgaaa aaatatattt tcacagaaaa gacagggaga tcgggaaatc ccgctggact
     1081 ctttccaaga agtttaagct tgtgatggat tccatgatga gtttttccta ttttccgata
     1141 cgatttatgt caggagtcgg tgtgatctgt gcgatcattg cgtttttgtg gatcgtagag
     1201 gtgatcctgg aacgtctgat cattggaacg ccgatcgaag gctgggcatc actgatgtgc
     1261 ctgattttgt tttctaccgg catggtcctt ctgatgcttg gcattcttgg ggaatactta
     1321 tggaggacac ttgatgcgag ccgtaatcgt ccgccgtttc ttgtggatac ggtcaacgag
     1381 tgcagacgga acggaacttc tgaatcggac gaaaagatat agcggagtat ggacggaatg
     1441 gaacagaaag aaaaaaagga aggcattctt acaggcttta tccagtattt ttacggtaat
     1501 tttgttgtac tgctgctggg atttgtttcg ctgcctctga tcacacgtgt gatgacaacg
     1561 gatgaatatg gcagaacggc aatgtttacg tcggctgttt ccatgattta catttttgcc
     1621 attttaggtc tggatcagtc ttatattcgt tatttttata aggaaggcgt aaacagaaaa
     1681 aagcttttta tgcagtgtct ccgtttcccg gtgattctga tcgtgctgct ggcagggctg
     1741 tatgtgcttg gagccaggta ttttaacgga tttttgttcg gacgaagcgc ccttgatatc
     1801 acactgcttg ttattgctta tacgatcata tccgtgtttg agcgatttct gtttttaaat
     1861 atcaggatgg agcagaacgg aaagctgtac tcaaatctga atattttatc caaggtactt
     1921 tatattttat ttattttgct gtttgtcgca tttctgggaa atgattttcg ggtcgttctg
     1981 tatgcgatga cgttgtcgca tacagaacga cccgaaaatc atttcccaga aatgcgacaa
     2041 acagcaaaa
//
LOCUS       DAOSYE010000413         2042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45147_length_2042_cov_3.106190, whole genome shotgun sequence.
ACCESSION   DAOSYE010000413 DAOSYE010000000
VERSION     DAOSYE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_2042_cov_3.106190"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLU43_07520"
     CDS             <1..245
                     /locus_tag="PLU43_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOO28298.1"
                     /translation="KETLHYRLSDEGIEITVGDAKESQAWADIHKAVSTNKSIIVYTN
                     KVNASIFPRVDVGDKITDVIEMISTHMPPKKVNIRI"
     gene            262..708
                     /gene="tsaE"
                     /locus_tag="PLU43_07525"
     CDS             262..708
                     /gene="tsaE"
                     /locus_tag="PLU43_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOO28299.1"
                     /translation="MIIETYNREETYALGKALGKEAVPGSIYTLVGELGTGKTVLTQG
                     FAEGLGIAGPVSSPTFTILQVYEDGRLPFYHFDVYRIGDIEEMDEIGYEDCFYGTGVC
                     MIEWADLITELIPENAHWITIEKELDKGPYYRLIKIDEYEIRDSSL"
     gene            722..1435
                     /gene="tsaB"
                     /locus_tag="PLU43_07530"
     CDS             722..1435
                     /gene="tsaB"
                     /locus_tag="PLU43_07530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOO28300.1"
                     /translation="MKILAIDSSGLVASVAIMEDTRLLAEYTVNYKKTHSQTLVPMLS
                     EIVKMIELDLSEIDAIAVAKGPGSFTGLRIGSATAKGLGLALDKPIIAVPTVDALSFN
                     AWGTNMLVCPLMDARRSQVYTGIYEFPEGKHHIIKEQCAIAIDDLIKELNGIGRKVLF
                     LGDGVPVYRDDLMERLHVPYEFAPAHMAGQRAGALGVLAFAYFKEGKIETAAEHKPDY
                     LRLSQAERERMEKNRLGRA"
     gene            1487..1930
                     /gene="rimI"
                     /locus_tag="PLU43_07535"
     CDS             1487..1930
                     /gene="rimI"
                     /locus_tag="PLU43_07535"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOO28301.1"
                     /translation="MIKIREMLESDIADIARIEEETFSMPWSADAFHDMLVRDNMTYL
                     VIDLNGQIIGGAGIRNILGDAEITNVAIKKSYRNKGYGKRLINALTNKGYEMGANAFT
                     LEVRKSNIPAILLYKSLGFTEEGLRKNFYEKPREDALIMWKRQSV"
BASE COUNT          605 a          347 c          579 g          511 t
ORIGIN      
        1 ttaaggaaac gcttcactac agactttcgg atgagggaat tgagattacc gttggtgatg
       61 caaaagaatc acaggcatgg gctgatatac ataaagccgt atcgacaaat aaaagtatta
      121 ttgtatatac aaataaagtg aatgccagta tttttccaag agtggatgtg ggagacaaaa
      181 taacggatgt gatcgagatg atttcgaccc atatgccgcc aaagaaagtg aatatcagaa
      241 tatgagccgg cggagacagg tatgataata gaaacatata acagggaaga aacgtatgcg
      301 cttggtaaag cgcttggtaa agaagctgtg ccaggaagca tttatacgct ggttggtgaa
      361 cttggaacag gaaagacagt gctgacacag ggatttgcgg aaggacttgg tattgccggc
      421 ccggtcagca gtccgacctt tacgattctt caggtctatg aggacggaag gctgcctttt
      481 tatcattttg atgtttaccg gatcggtgat attgaggaaa tggacgagat cggatatgag
      541 gattgttttt atggaacagg cgtctgcatg atcgagtggg ctgatctgat aactgagctg
      601 ataccggaaa atgcgcattg gattacgatc gaaaaagaat tggacaaagg gccttattac
      661 cggctgatca agatagatga gtatgaaata cgggacagtt ccctgtaaag aggcagttat
      721 tatgaagatt ttagcaattg acagttcagg actcgtagca tctgtggcaa ttatggaaga
      781 tacgcgtttg cttgcggaat atacggtaaa ttataagaaa actcattcgc agacattagt
      841 gccgatgctg agcgagatcg taaagatgat agaactggat ttgtccgaaa tagatgcgat
      901 tgcggtcgca aagggtccgg gttcgttcac aggacttcgg atcggttctg cgacggcaaa
      961 ggggcttggg cttgcgcttg ataaaccgat tattgcagta ccgacggtcg acgcacttag
     1021 ttttaatgca tggggtacca atatgcttgt ctgtccgctc atggatgcaa gaagatccca
     1081 ggtttataca ggaatttatg agttcccgga agggaaacat catattataa aagaacaatg
     1141 tgcgatcgca attgatgacc tcattaaaga actgaacgga atcgggagaa aggtgctgtt
     1201 tttaggagac ggggtgccgg tctatcggga cgatcttatg gagaggctgc atgttccata
     1261 tgaatttgca cctgcgcata tggccgggca gcgtgccggt gcgctggggg ttttggcatt
     1321 tgcttatttt aaagaaggaa agatcgagac cgcagcagaa cataagccgg attatttgcg
     1381 gctttcacag gcagaacggg aacgtatgga gaaaaacagg cttggacggg cctgacgata
     1441 tgataaatga cggcggcaat tgcggccgga gacgggggat aaggatatga taaagatacg
     1501 ggagatgctg gagagcgata tcgctgatat tgccaggatc gaggaggaga cgttttccat
     1561 gccttggtct gcggatgcgt ttcacgacat gctggttcgg gataatatga cttatcttgt
     1621 aattgatctg aatggtcaga tcatcggcgg tgccggtatc cgtaatattc tgggagatgc
     1681 agaaatcaca aatgtagcga tcaagaagag ttacaggaac aaaggctatg gaaaacgtct
     1741 gatcaatgcc ctgaccaata aagggtatga gatgggggcc aatgcattta ctctggaagt
     1801 gagaaaaagt aatataccgg ctattttact ttataaaagc cttggattta cagaagaggg
     1861 gcttcgaaag aatttttatg aaaagccaag agaggacgcc ctgatcatgt ggaaaagaca
     1921 gtctgtttaa aacagctgca tggtctggcg cagacagtga ggaaatgaaa tgctggatgt
     1981 ttgtttgtta ggaaccggcg gtatgatgcc actgccatat cgatggctga cgtctcttat
     2041 gt
//
LOCUS       DAOSYE010000414         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45205_length_2041_cov_2.161128, whole genome shotgun sequence.
ACCESSION   DAOSYE010000414 DAOSYE010000000
VERSION     DAOSYE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45205_length_2041_cov_2.161128"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLU43_07540"
     CDS             complement(<1..484)
                     /locus_tag="PLU43_07540"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO28302.1"
                     /translation="MAIDLFTIGRFTIHGYGLMIGIGFLAAVLVGCYRAKRYGLSDDH
                     LINIALLGLVAGFLGSKILFVIVEFRTFLEDPLEVLGSEGFVVYGGIIGGTLAMYLYC
                     RIRKLKFLSYFDIVTPSVALNQAFGRIGCFLAGCCYGKETHSVFGVIFPSGCLAPSGV
                     K"
     gene            complement(601..1221)
                     /locus_tag="PLU43_07545"
     CDS             complement(601..1221)
                     /locus_tag="PLU43_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOO28303.1"
                     /translation="MKTYLTPEEIEARSFAIIEEELGRELPFMEKPVIKRVIHTTADF
                     SYADTMCFSENAVEKGLCALQEGADIVTDTNMGMAGINKKKLKEFGGKTYCFMADESV
                     AIEAKSRHITRASVSMERTAAMEQNLIYAIGNAPTALFTLYDLIREGKIRPRLIIGVP
                     VGFVNVTEAKELIMTAGVPYIVARGRKGGSNVAAAICNALLYFYGK"
     gene            complement(1265..>2041)
                     /locus_tag="PLU43_07550"
     CDS             complement(1265..>2041)
                     /locus_tag="PLU43_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HOO28304.1"
                     /translation="PDEEKIIAAVILLPHISNYTDFSLLAAYEGVSLSYIKNVRELGR
                     PDLVILPGSKNTMGDLLWLRQSGLEASIQRLAKEGKRIIGICGGYQMLGKTIRDPDCM
                     EHGGLLRGIGLLPAATCFGNKKHRTRVTGKIEKQETCYQTLSHVEFEGYEIHMGDTVL
                     EEGATARSFAVLKDSVNNTCYNDGYVKDNILGTYVHGILDSPQLLKALLTPLFEEKGL
                     QMEAVEYEDHYRYKLRQYDLLADVIREHMDMKAVYEMLGL"
BASE COUNT          523 a          568 c          386 g          564 t
ORIGIN      
        1 gcttcacccc tgacggagcc agacatcctg acgggaaaat aacaccgaac accgaatgcg
       61 tctctttccc ataacagcag cccgccaaaa aacagccgat cctgccgaat gcctgattga
      121 gtgcgaccga tggggtgacg atatcaaaat agcttaaaaa tttcagtttc ctgatccgac
      181 agtacaaata catggcaagg gtcccgccga tgatgccccc gtatacaaca aatccttccg
      241 agccaagtac ctcaagcgga tcttctaaaa aggtcctgaa ttccacaatt acaaacagga
      301 tcttgcttcc aagaaatccg gccaccaggc ccagcagtgc aatattgatc agatggtcgt
      361 cactgagtcc atatcttttc gccctgtagc agccaacaag gaccgccgct aaaaacccga
      421 ttccgatcat cagaccatag ccgtgtatcg taaatctgcc tattgtaaat aaatcgattg
      481 ccatatcttt tgctcctttt tccatactct gtacattatt cgccagtgta tttttctgat
      541 acagtttcct gtgttcatta tattttttgc acggaaaaat gtcaacaatc catggcagct
      601 ctattttcca taaaaatata agagcgcatt gcagatggct gccgccacat tgcttccgcc
      661 ttttctgccg cgcgccacga tatacggtac acctgccgtc atgatcagct ccttggcttc
      721 tgttacattg acaaagccta ccggtacgcc aatgatcaga cgcggccgga tctttccttc
      781 cctgatcaga tcatacagcg taaaaagcgc agtcggtgca tttccgatcg catagatcag
      841 attctgttcc atcgccgccg tacgctccat actgacggaa gccctcgtga tatgccggct
      901 tttcgcttct atggcaacgc tttcatctgc cataaagcaa taggtctttc caccgaattc
      961 cttcagcttt ttcttgttga tgccggccat tcccatattc gtatctgtaa caatatcggc
     1021 gccttcctgc agcgcacaca gacctttttc cactgcattt tcagaaaaac acatcgtatc
     1081 cgcataggaa aaatcagcgg tcgtatggat cacgcgctta atgacaggtt tctccataaa
     1141 cggaagctcc ctgccaagtt cctcttcaat gatcgcaaag ctgcgtgctt cgatctcttc
     1201 cggtgttaaa taagttttca tgcaagcctc ctcatcatcg acaatttctt tccatgcagc
     1261 atctttacag accgagcatt tcatataccg ctttcatatc catatgttcc ctgatcacat
     1321 ctgcaagcag atcatactgt cttagcttat agcggtaatg atcttcatat tccacggctt
     1381 ccatctgcag gcctttttct tcaaataaag gtgtcagcag tgccttaagc agctgcgggc
     1441 tgtccaggat accatgcaca taggtgccaa gaatattatc tttcacatat ccatcattat
     1501 aacatgtgtt atttacgctg tctttcagca ctgcaaacga tcgcgctgtc gctccctctt
     1561 ccaaaacggt atcacccata tggatctcat agccttcgaa ttccacatga ctgagtgtct
     1621 ggtaacaggt ttcctgtttt tcgatctttc ctgtcacccg tgtccggtgt tttttatttc
     1681 caaagcatgt cgccgcaggc aaaagcccaa tcccacgaag aagaccgccg tgttccatgc
     1741 aatccggatc acggatcgtt tttccaagca tctgatagcc tccgcagata ccaatgatcc
     1801 gcttcccttc tttggcaagc cgttgtatgg aagcttccag gccgctctgt ctaagccata
     1861 gcagatcacc cattgtattc ttactgcccg gcagaatcac aagatccggt ctgccaagct
     1921 cccgcacgtt ttttatatag gaaagggaaa cgccttcata tgcggcaagc agcgaaaaat
     1981 cagtataatt tgaaatatgc ggcaaaagaa taacagccgc aattattttt tcttcatcag
     2041 g
//
LOCUS       DAOSYE010000415         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45287_length_2038_cov_3.502269, whole genome shotgun sequence.
ACCESSION   DAOSYE010000415 DAOSYE010000000
VERSION     DAOSYE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45287_length_2038_cov_3.502269"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PLU43_07555"
     CDS             <1..1684
                     /locus_tag="PLU43_07555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO28305.1"
                     /translation="IIVSFISVTVSVLTVYSISKKTVQNQYIKLAEDNLAAFDIVVDK
                     DLETLIGTIRNYILDDKIISTISKEPQTQNGHYFDSGSRTQLFDQVSEMFNQLSMVEG
                     VFIFDNYDRYYMYLKNGKNTSAYLNYYNVGIDKDSDWYRAMMETRGKEIFWNGDVLNP
                     DNQDCFTIIKKLNDTTKYDPVGVVVITVRKTFLKEIYSELNTQNIVLLLDESDNYITQ
                     IGTSNSTDDFFEAYKKEKYEDDGDYLYLERINTTTGWKFITGIRKNKLYEENIYIRSY
                     IEILLLIVVGAVFLVSILVTNRIYKPLRKLEEAVEEINCGKRQIETEFDDSEIGTIGQ
                     TIKRVVNNNLYLEKQIVEIELSKKKAQFLLLQAQINPHYLYNTLDSIYILALKYKAED
                     IAQMVLSLSEMFKLSLNEGKGYVHVQEEINYVKNYMCVMNYRFKNRFKVFFDVDDEIL
                     DCYMLKFLLQPFVENSIIHGLEQKVGEGSVTISGMRSDGNLEFKIYDDGIGFDVENEH
                     TTGYGIRNVIERIELVYGKGYGVKIRSTIGEGTTVIIKIPDKGHEFYYQSES"
     gene            1850..>2038
                     /locus_tag="PLU43_07560"
     CDS             1850..>2038
                     /locus_tag="PLU43_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO28306.1"
                     /translation="MKKKFLSILLSIAMVSVMLAGCGSTAETEESTDTAVTEEDTTAA
                     AESSDDTTAADTAAESRTI"
BASE COUNT          678 a          305 c          495 g          560 t
ORIGIN      
        1 cattatcgta tcgtttattt ctgtaaccgt atcggtattg actgtctaca gtatctctaa
       61 aaaaaccgta cagaatcagt atattaaact ggcagaagac aatctggctg cttttgatat
      121 tgtagtggat aaggatctgg aaaccttgat cggtacgatc cggaattata tattggacga
      181 taagatcatc agtacgatca gtaaagagcc gcagacgcag aatggtcatt attttgattc
      241 cggcagcaga acgcagcttt ttgatcaggt ctcggaaatg ttcaatcagc tcagcatggt
      301 ggaaggcgtg tttatttttg ataattatga ccgctattat atgtatttaa agaacggaaa
      361 gaatacatcg gcgtatttga attactacaa tgtcgggatc gataaggaca gtgactggta
      421 ccgtgcgatg atggaaacaa gggggaaaga gatcttctgg aacggagatg tgctgaatcc
      481 ggacaatcag gactgcttta cgatcataaa aaagttaaat gatacgacaa aatacgatcc
      541 ggtgggcgtt gtggtaatta cggtcagaaa aactttttta aaggagatct acagtgagct
      601 gaatacacag aatatcgtgc ttctgctgga tgaatccgat aattatatta cacagatcgg
      661 aacaagcaat agtacggatg atttttttga agcatataaa aaagaaaaat atgaggatga
      721 cggcgattat ttatatctgg agcggatcaa tacgacgacc ggctggaagt ttattaccgg
      781 tatccgaaaa aataaattgt atgaagaaaa catttatatc aggtcctata tagagatcct
      841 gctgcttatt gtcgtggggg ccgtattttt agtgtcgatc ttagtgacga acaggatcta
      901 caagccgctt cgaaagcttg aagaggctgt ggaagaaata aactgcggaa aacggcagat
      961 cgaaacagaa tttgacgaca gtgagatcgg aacgatcggg cagacgatca aacgcgtggt
     1021 caataataat ctgtatctgg aaaagcagat cgtcgaaata gagctgagca agaaaaaagc
     1081 gcagttttta ctgctccagg cgcagatcaa tccgcattat ctgtataata cgctggattc
     1141 catttatatt ctggcgctga aatataaagc ggaggatatt gcccaaatgg tgctgtccct
     1201 gtcggaaatg tttaagctgt ctctgaatga aggaaaggga tatgttcatg tgcaggaaga
     1261 gatcaattat gtgaaaaatt atatgtgtgt gatgaattat cgttttaaaa acaggtttaa
     1321 ggtatttttt gatgttgatg acgagatcct ggattgttat atgttgaagt ttttattgca
     1381 gccgttcgtt gaaaattcca ttatccatgg tctggaacag aaagtcggag aaggcagtgt
     1441 gacgatttcc ggtatgcgtt cggatggaaa tctggaattt aagatctatg atgacgggat
     1501 cggatttgat gtggagaacg agcacacgac aggctatgga atcagaaatg taattgaaag
     1561 gatcgaactg gtttatggaa aagggtacgg ggtaaagatc agaagcacga tcggagaagg
     1621 tacgacggtg atcataaaga tcccggataa gggtcatgaa ttttattatc agagtgaatc
     1681 ttaattttct gtacattata tttctttttc agacatgaaa cttaaataat atgacaaatt
     1741 ataaagaatg aggctctata aaaaaacatc atagtaaggt ataaataaat tataaagttt
     1801 tcgaaaactt atgcagcatg tcatttatta taagtgtagg aggactatga tgaaaaagaa
     1861 atttttatca attttattaa gtattgcaat ggtttcggtt atgcttgcag gatgcggaag
     1921 tacggctgaa acagaagaaa gtacggatac ggccgtaacg gaagaagaca cgacggcagc
     1981 ggcagagagc agtgatgata caacagcagc tgatacggca gcagaatcca gaacaatt
//
LOCUS       DAOSYE010000416         2037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45340_length_2037_cov_2.239657, whole genome shotgun sequence.
ACCESSION   DAOSYE010000416 DAOSYE010000000
VERSION     DAOSYE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45340_length_2037_cov_2.239657"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..486
                     /locus_tag="PLU43_07565"
     CDS             34..486
                     /locus_tag="PLU43_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28307.1"
                     /translation="MDENQFHDDEAFYQENKQADQGKVAAAKTLAAASMILGILSIVS
                     VFCFIPFVFSAIGITLAILSKGASKYYSNQGRFGLLCSIIGLAASFILTIFVVVVEFL
                     TLFSGTNGTGSLWENVEQKYEQQYEDVYGKEMPQEFKDLFQELENADE"
     gene            526..1320
                     /locus_tag="PLU43_07570"
     CDS             526..1320
                     /locus_tag="PLU43_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOO28308.1"
                     /translation="MKRQKISSDLKAMAKEQLLGNYGIAAGTFAILYGIIIGVVILVY
                     LAVALTILTGGNVSQASDLEAIALSPGYYIMMLFVTALLGALFSTLFVGYANIALKIA
                     RGDKPKIADLFYCYSHHPDKVIILYLMLYAVNFVLMLPAQITAFFMEGTEAASGVQFL
                     IWAVLYTAGLVLSVIFSLMVSQVYFLYVDDSMENTIDIFKQSIRIMKKNKGRLFYIML
                     SFLGWYLLILASCGIAAFWIIPYQAVTLADFYRDLAGEFNENADEN"
     gene            1341..>2037
                     /locus_tag="PLU43_07575"
     CDS             1341..>2037
                     /locus_tag="PLU43_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOO28309.1"
                     /translation="MDIIKKLKDELKVEKWQVEAAVKLIDEGNTIPFISRYRKEATGS
                     LNDEVLRDLNERLTYLRNLEEKKEQVLSSIKEQGKLTEELKTQILAAETLVVVDDLYR
                     PYRPKRRTRATIAKEKGLDPLAELIYAQQTDIPLEESAKEYINEETEVLTAEEAIAGA
                     KDILAEKISDEADYRRYIRQATFESGRIVSAARNEKQESVFEMYYQFEEAVKKIAGHR
                     VLALNRGEAEKILT"
BASE COUNT          602 a          350 c          518 g          567 t
ORIGIN      
        1 cttgaggact gattttatat aaggggagcg gcgatggacg aaaatcaatt tcatgacgac
       61 gaagcctttt atcaggaaaa taagcaggca gatcagggaa aagtagcagc tgcaaaaaca
      121 cttgcggcgg catccatgat ccttgggatt ctgtcgatcg tctcggtctt ctgctttata
      181 ccgtttgtat tcagcgcgat cgggataaca cttgccatat tatcaaaggg tgcgtcaaaa
      241 tattattcaa atcagggcag attcggcttg ctctgctcta ttatcggtct cgcggcttct
      301 tttattttaa cgatatttgt tgttgtcgta gagtttttga cgctgttttc aggcacgaac
      361 ggaaccggtt cgctttggga aaacgttgaa caaaaatatg aacagcagta tgaggatgtg
      421 tatggaaagg aaatgccgca ggaattcaag gatctgtttc aggagctgga aaatgcagat
      481 gaatgatcat ttccaatact ttagttcgga taagaggaga gtattatgaa aaggcaaaaa
      541 atatcatcgg atttaaaagc aatggcaaag gaacagttac ttggcaatta cggaattgca
      601 gcaggtacct ttgctattct ttatggaatc ataattggtg ttgttatact tgtatatctt
      661 gcagttgccc tgacgatact tacagggggg aatgtgagtc aggcttccga tctggaagcg
      721 atcgcattat ctccgggtta ttatattatg atgctttttg tgaccgcatt gctgggagcg
      781 ctgttcagca ctctgtttgt gggttatgcc aatatcgctc tgaaaatagc aagaggagac
      841 aaaccgaaaa tagcagatct tttttattgt tattcccatc atccggataa agtgatcatt
      901 ctttatctga tgctgtatgc ggtcaatttc gtattgatgc tgcctgcaca gatcactgca
      961 ttttttatgg aaggaacgga agcggcctcc ggtgtgcagt ttttgatctg ggccgttctt
     1021 tatactgccg gtcttgtgtt gtctgttatt tttagtctga tggtttccca ggtatatttt
     1081 ctctatgtgg acgattcaat ggagaacacg atcgatatct ttaaacagag cattcggatc
     1141 atgaaaaaaa acaagggccg gttattttat atcatgttaa gttttctcgg ctggtacttg
     1201 ctgattttgg cttcctgcgg aatcgcagct ttttggataa ttccgtatca ggcagtgacg
     1261 cttgcggact tttacaggga tcttgcaggg gaatttaatg agaatgcaga tgaaaattaa
     1321 tgaacaggaa ttgataaata atggatatta taaaaaaatt aaaagatgag ctcaaggtgg
     1381 agaaatggca ggtagaggct gccgtaaagc tgattgatga gggaaatacg attcctttta
     1441 tttcccgtta caggaaagaa gcaacaggct ctttaaatga tgaagtttta agagatctga
     1501 atgagaggct gacctatctt cggaatctgg aagagaaaaa ggaacaggtg ctttcgagta
     1561 ttaaagagca gggaaagctg acagaggagc tgaaaacaca gatactcgcg gcggagacgc
     1621 ttgtcgtcgt ggatgatctg tacaggccgt accgtccgaa aagaagaaca agggcgacga
     1681 ttgcaaagga aaaaggcctg gatccgcttg cggaactgat ctatgcacaa cagacagata
     1741 ttccgttaga agagagtgca aaagaatata tcaatgagga aacagaagtt ttaactgcag
     1801 aagaagcgat cgcaggcgcg aaggatattc ttgccgaaaa aatttccgat gaggcagact
     1861 acagaaggta tatccggcag gcgacctttg agtccggcag gatcgtatct gctgccagaa
     1921 atgaaaagca ggaatctgta tttgaaatgt actatcagtt tgaagaagca gtcaaaaaaa
     1981 ttgcaggaca tcgtgtgctt gcgttaaacc gcggagaggc ggaaaagata ctgaccg
//
LOCUS       DAOSYE010000417         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45370_length_2036_cov_3.552246, whole genome shotgun sequence.
ACCESSION   DAOSYE010000417 DAOSYE010000000
VERSION     DAOSYE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45370_length_2036_cov_3.552246"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLU43_07580"
     CDS             complement(<1..1104)
                     /locus_tag="PLU43_07580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO28310.1"
                     /translation="MKDFEMIDDYAELEEKQKAPLFRDVTAVSLDENMENEFTGQGNN
                     EEIQQPVISQAASRIMASMDKLENRRVLLINTIVGSGSVGRIVSGLYRTLNEHGYECL
                     VAYGRGEAPDSIRSYRIGTDTGVYIHGGLSRITDRHGFFSSKATIELIAVIEEFNPDI
                     VHLHNVHGYYLNVKLLFEYLKRSGRRVIWTLHDCWSFTGHCTHFEYIGCQKWQNGCFQ
                     CEQLQEYPKSLMVDNSKKNYQQKKELFTGFSNMTIVTPSEWLEARVKQSYLQKYHTVV
                     IPTGIDLEQFAPVEEERTDDNLVFQLRTRLGIKNKHVLLGVANPWRERKGLSQFESLA
                     KAISDKFVIILIGLNDDQLSSLPHSIIGFSKTES"
     gene            complement(1167..>2036)
                     /locus_tag="PLU43_07585"
     CDS             complement(1167..>2036)
                     /locus_tag="PLU43_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HOO28311.1"
                     /translation="QESDIGIARIREANFPIVTVEDETEFEKILEKEQPDILVNDILD
                     TSADYMAMVTKYAKRVVNFEDVGPGAKYADAVINALYEKGEKLHNEYYGSKYYCIRDE
                     FLEEKPKEFSEEVKNILVIFGGADPSDLTGRLYDICKVLHGKHPKIQYHFLLGFAYRH
                     KDKIVTDEANNIFVHNDVKRVSAYMSKMDLAITSQGRTVYELASMGVPSIVLAQNERE
                     AEHVFAGIQNGFINLGIGREQDALTIISTIRWLLSTPKVRQEMRKLQLTKDFSKGQER
                     VIRLIMNDPDDED"
BASE COUNT          489 a          555 c          396 g          596 t
ORIGIN      
        1 gctctctgtt ttggaaaaac caataatcga atgcggcaga ctcgaaagct ggtcgtcatt
       61 cagcccgatc agtatgatca caaacttatc gctgatcgcc tttgccagcg attcaaactg
      121 agacagacct tttctttctc tccacggatt tgccacacca agcaggacat gcttgttctt
      181 gatgccaagc cttgtccgaa gctgaaatac caggttatcg tcggtgcgct cttcttctac
      241 cggcgcaaac tgctccagat cgatcccggt cggaatcacg accgtatggt atttctgtaa
      301 ataggactgc ttcacacgtg cttccagcca ttccgacggc gttacgatcg tcatattgga
      361 aaaaccggta aacagttcct tcttctgctg gtaatttttc tttgaattgt ctaccatcag
      421 cgattttggg tattcctgca gctgttcaca ctgaaaacag ccgttctgcc atttctggca
      481 gccgatgtat tcaaaatgcg tacagtgtcc cgtaaagctc cagcagtcat gcagcgtcca
      541 gatgaccctt cttcccgacc gctttaaata ctcaaacagc agcttcacat tcagataata
      601 accatggaca ttgtgcagat gtacaatatc cggattaaac tcttcaatga ccgctatcag
      661 ctctattgtt gcctttgaag agaaaaaccc atgcctgtct gtaatcctgc tcagcccgcc
      721 gtgaatgtag acacctgtgt ccgtgccgat ccgataggaa cggatgctgt cgggcgcttc
      781 acctcttccg taagcaacca gacattcata tccatgttcg ttcagtgtcc tgtacagacc
      841 gctgacgatc ctgccgacac tgccggaacc gacgatcgta ttgatcagca ggacacgcct
      901 gttctcaagc ttatccatag acgccatgat cctgcttgca gcctgactga tcacgggctg
      961 ctgtatttct tcattatttc cctgtccggt aaattcattt tccatattct catccagaga
     1021 aactgccgta acatccctga acaaaggagc tttctgtttt tcttcaagct ccgcataatc
     1081 atcgatcatt tcaaaatcct tcatgggaac ctcttctttc ttttaaatcc gatgtgcaga
     1141 ccggccggat ccattttgaa atccgctcag tcttcatcgt ccgggtcgtt cataatcaat
     1201 cgaatgactc tctcctgccc tttggaaaaa tccttagtca gctgcagctt tctcatttcc
     1261 tgtcttacct ttggtgtgga aagcagccat ctgatcgtgg aaatgatcgt cagggcatcc
     1321 tgttctctgc cgatgccaag attgataaaa ccattctgaa tacctgcaaa cacatgctct
     1381 gcctcccgtt cgttctgtgc cagtacaatg gacggcacac ccatgctcgc aagctcataa
     1441 acagtccttc cctgcgaggt gatcgcaaga tccatcttgc tcatgtaggc actgactctc
     1501 tttacatcgt tatgaacaaa aatattgttt gcctcatcgg tcacgatctt gtctttatga
     1561 cggtaggcaa agccgagcag aaaatgatat tggatctttg gatgcttgcc gtgcagcacc
     1621 ttgcatatat catataatct gcccgtcaga tcggaaggat cggctcctcc gaagatcaca
     1681 aggatatttt tcacttcctc ggaaaattct tttggtttct cttctaaaaa ttcatccctg
     1741 atacagtaat acttagaacc gtaatattcg ttatgcagct tttctccctt ttcatacagt
     1801 gcattgatga ccgcatccgc atatttggca ccgggaccga cgtcttcaaa attaacgaca
     1861 cgcttcgcgt acttggtcac catcgccata tagtctgcgg aggtgtccag aatatcgtta
     1921 acgagaatat ccggctgctc tttttccaat attttttcaa attccgtctc atcttccact
     1981 gtcacgatcg gaaaattggc ttccctgatc cttgcgatcc cgatatcact ctcctg
//
LOCUS       DAOSYE010000418         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45416_length_2035_cov_2.587374, whole genome shotgun sequence.
ACCESSION   DAOSYE010000418 DAOSYE010000000
VERSION     DAOSYE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45416_length_2035_cov_2.587374"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1021
                     /locus_tag="PLU43_07590"
     CDS             152..1021
                     /locus_tag="PLU43_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28312.1"
                     /translation="MQTLNVFEKGFGYLEACLAFYSENCIYFLLYIAAIAVIVLKGTK
                     KEKQIFVPGAVLLLLTVYNPAAPVVLDKIFDVNSEYYRFFWIAPIIVLLPYMAAKLIM
                     SGENKGQKAVIALLLAAICVLGGNFLYENGYVSAANQYKMPEEMIQISELIHEDTAVE
                     YPKAFLEYEYNMQMRQYDPKMLLTIDREDYLYAVSSPSYTDEMIADDKNPQFRLLAAL
                     VRYQQVDSDAFLAALDATGTEYVVLSADNPMAVYLKKAGLTVIGSTATHTIYKYKLKD
                     PTVFELVDYSVVY"
     gene            1043..1915
                     /locus_tag="PLU43_07595"
     CDS             1043..1915
                     /locus_tag="PLU43_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28313.1"
                     /translation="MLLTVFTPTYNREGLLKRAYESLLNQTDGNFCWLIVDDGSTDNT
                     RELVADWIKEGRLQIRYEYCRNGGKMRAHNTGVRLCETELFLCLDSDDYLTKTAVEEI
                     SACWREAVKTDGKKMPAGIVAYKGKSETELLSGTEFPYTKDTRLYHLYLNGFRGETTL
                     IFRTDILKEFPFPEIEGEKYVPEDYIYDKIDTEHVLRVLPRIITVCELVSSGYTDSVA
                     GLKQDNPNAWFLYYEQRARITPFSVLKLKYLAYYIVYAKRCKKPLFKATGMPFFFVLL
                     GLPGALVLKLTGRK"
BASE COUNT          547 a          381 c          527 g          580 t
ORIGIN      
        1 tatgtgctgt gcatgcttcc ggagctgatc atggcggtaa catatgttgc ctatgtcaaa
       61 gggtattttg tattgtatac gtacccgcgc tagttttccg gccgtttatc aggcctgttt
      121 taaagattgt tgattctata ggtggacaga gatgcaaaca ttaaatgtat ttgaaaaagg
      181 gtttggctac ctggaagcgt gccttgcctt ttattccgaa aattgtatat attttttatt
      241 gtatattgca gccatagcgg tgatcgttct gaaagggacg aaaaaggaga aacagatctt
      301 tgttccgggt gcggttcttt tgctgctgac tgtttataat ccggcggcgc cggtagtgct
      361 tgacaagatt tttgatgtga acagtgaata ttaccgtttc ttctggatcg caccgatcat
      421 cgtgctgctt ccatatatgg cggcgaagct gatcatgtca ggggaaaata aagggcagaa
      481 ggctgttatt gcactgcttc ttgcggcgat ctgtgtattg gggggtaatt ttctttatga
      541 aaacggctat gtgtctgcgg ccaatcaata caaaatgccg gaagaaatga tacagatctc
      601 ggaactgatc catgaggata ctgctgttga atatccgaaa gcatttctgg aatatgaata
      661 taatatgcag atgcgtcagt atgacccgaa aatgctgctt acgatcgatc gggaagatta
      721 tctttatgca gtcagttctc cttcgtacac ggatgaaatg atcgcagatg ataaaaatcc
      781 gcagtttcgg ctgcttgccg cacttgtccg gtaccagcag gtcgattctg atgcctttct
      841 tgccgcactt gatgcaacgg ggacagagta tgttgttctt tccgcagata atccgatggc
      901 tgtctatctg aaaaaagcag gtctgaccgt gatcggcagt acggccactc atacgatcta
      961 caaatataag ctgaaagatc cgacagtatt tgaactggtg gactattctg tagtatactg
     1021 aacaggaacc ggaggaatct atatgctgct gacagttttc acaccgacat ataaccgtga
     1081 aggtcttctt aaaagagcat atgaaagtct tttgaaccag acggacggca atttctgctg
     1141 gctgatcgtt gacgatggtt ctaccgacaa cacgcgggag cttgtggcgg actggataaa
     1201 agagggccgg ctgcagatac gctatgaata ttgcaggaac ggcggaaaga tgcgtgcaca
     1261 taacacaggc gtgcgtcttt gcgaaacgga gcttttttta tgtctggatt cggatgatta
     1321 tctgacgaaa acggcagtgg aggagatttc ggcatgctgg agggaagcgg tgaagacgga
     1381 cggcaaaaaa atgccggcag ggatcgttgc ctataaggga aaaagcgaga cggagctttt
     1441 atcagggacc gaatttccct atacgaagga tacgcggctg tatcatttgt atttaaatgg
     1501 ttttcgcggg gaaacgacgc ttattttcag gacagatata ttaaaagaat tcccgtttcc
     1561 ggaaatagaa ggcgaaaaat atgtaccgga agactatatt tatgataaaa tagatacaga
     1621 acatgtattg cgtgtattac cgcggatcat tactgtctgc gaacttgttt caagcgggta
     1681 tacggattca gttgccggac tgaagcagga caatccgaat gcatggtttc tgtactatga
     1741 acagcgcgca aggattacgc cgttttcggt gttgaaactg aaatatcttg cttattatat
     1801 tgtttatgcg aagaggtgta aaaagcccct ttttaaagcg accggcatgc cgtttttttt
     1861 cgtgctgctc ggacttcccg gcgcactcgt actgaagctg accggcagaa aatgaggtgt
     1921 ttttttgaac aggacacaaa tgtggaaaag gatatacagt tttttcatgg cttttttgat
     1981 cgtagcggtt gaaacgggca ttgtgacata tgtctgggtg cgatactata atact
//
LOCUS       DAOSYE010000419         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45519_length_2032_cov_3.124431, whole genome shotgun sequence.
ACCESSION   DAOSYE010000419 DAOSYE010000000
VERSION     DAOSYE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45519_length_2032_cov_3.124431"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLU43_07600"
     CDS             complement(<1..523)
                     /locus_tag="PLU43_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO28314.1"
                     /translation="MFLQHLLNGIIIGGIYAMVALGYSMVYGVLQIVNWAHADVLMFG
                     TFIGMLLATKLGFPIIAMILLAACFTSVLGMAVERAAYRPIKKNRRMAVLVSALGMST
                     FLQNLAQLIFGSQTQPFSTIKKVTYQVGNVSFSNMQIIILSVTAGMLIILYILVYKTK
                     MGVAMRACSVSIEN"
     gene            complement(617..1759)
                     /locus_tag="PLU43_07605"
     CDS             complement(617..1759)
                     /locus_tag="PLU43_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28315.1"
                     /translation="MMRKKVFKRMTAGVLTAAMMATLLTGCGSKSASEDTPIYIGTSF
                     PLTGSVAADGMLIMDAIQLAVDQVNEEGGIKGRQVEIVKEDDEASPTSAAAVANKFVE
                     NDDILGVISSYNSSCMFAQVDVYAEAGLPSISPVATNPGLTGISDYFYRTAPNDADVG
                     AEGASFCSDLGWKNVALLYENDDYGLGIADEFRSNAEELGMSIATEQTFVYGETVDFS
                     TILTAVADSGADGMFIAGLVTESGMICEQKSSYGCADVGIVGANGLYSPAMLEYGDAV
                     EGLYVLGEFSAEADNELTKKFVSDYSEKYGEEPGNWSALAYDAVMVMMDAMERVDGDL
                     TRESINEALKTTDYEGVSGTYQFVDCNCTKSEMRFVVKDGKFEIYE"
BASE COUNT          552 a          541 c          365 g          574 t
ORIGIN      
        1 cattctcaat acttacggag caggcacgca ttgccacacc cattttcgtc ttataaacaa
       61 gaatatataa aataatcagc atgcctgccg taacacttaa aataatgatc tgcatattgg
      121 aaaaactgac gtttccaacc tgataagtca ccttttttat cgttgaaaag ggctgcgtct
      181 gactgccgaa gatcagctgt gcaaggttct gcaggaaagt agacatacca agagcactga
      241 caagcactgc cattcttctg tttttcttga ttggccggta cgccgcacgt tccacagcca
      301 taccaaggac agacgtaaaa caggcggcca gcaggatcat tgcaatgatc ggaaagccaa
      361 gctttgtagc gagcagcata ccgataaaag taccaaacat cagcacgtcc gcatgcgccc
      421 agtttacgat ctgcagtacg ccgtatacca tcgaatagcc aagtgcgacc attgcataaa
      481 tgccgcctat aataattccg ttcagtaaat gttggagaaa cataagaaac tcctccttct
      541 tatttaaatt gaaagggctg ccgcggctgc ttcacacagc agcccttttc ttctgctctg
      601 acagtaacgt tacttcctat tcgtaaattt caaactttcc gtcttttaca acaaaccgca
      661 tttccgactt tgtgcagtta cagtcaacaa actgatacgt tccggaaaca ccttcataat
      721 cggtcgtctt taaggcctcg ttgatcgatt ctcttgtcag atctccgtct actctctcca
      781 tcgcatccat catgaccatg accgcatcat aagcaagtgc cgaccagttt cccggctctt
      841 ccccatactt ttcactgtaa tcggaaacaa atttcttggt cagctcattg tcagcttccg
      901 cactgaattc tccaagtaca tataaacctt ctaccgcatc accatattca agcattgccg
      961 gtgaatacaa accgtttgct ccgacaattc caacatccgc acatccgtaa gaggatttct
     1021 gctcgcagat catacctgac tctgttacaa gacccgcaat aaacatacca tctgctccgg
     1081 aatctgccac tgccgtcaaa atcgtggaaa agtcaactgt ttcaccgtaa acaaatgtct
     1141 gctcagtagc aatgctcatt ccgagttctt ccgcgttgct cctgaattca tctgcaatgc
     1201 caagaccata atcgtcattt tcataaagca gtgctacatt tttccaccca agatcactgc
     1261 agaaggaagc accctcggct ccgacgtcag catcattcgg tgccgtacgg taaaaataat
     1321 cactgatgcc tgttaacccc ggattcgttg caacagggct gatgctcgga agacctgcct
     1381 ccgcatatac atctacctgt gcgaacatac aggaactgtt gtaagaagaa attacaccta
     1441 aaatatcatc attttccaca aacttatttg ccactgcagc tgcggatgtc ggagacgcct
     1501 catcgtcttc tttcacgatc tcgacctgtc tgcctttgat tcctccctct tcatttacct
     1561 ggtcaacggc aagctggata gcgtccatga tcagcattcc gtctgctgcc acactgcctg
     1621 tcagcggaaa agaagtaccg atatagatcg gcgtatcttc cgatgcggat ttggaaccgc
     1681 atccggtaag caatgttgcc atcattgcgg cagtcagaac acctgcagtc attcttttga
     1741 aaaccttttt tctcatcatc tctttcttcc tcctttagtt taatctctct ctgttggtgt
     1801 attccttttg ttttatagtc acggcggcaa ttggcatgcc ccggttctat ctgtaaaagc
     1861 aaataacaaa gtcttgcctg tgaaataaaa acagcgcatt gattatactc aatgcgccat
     1921 tacattttcg tgtggtccgt ctggtcatac cacttaattg atttttacaa atcatactgg
     1981 ataaattcca aaaatgcaat acctttttat ttgaaacgtc ttttttaaat tg
//
LOCUS       DAOSYE010000420         2026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45749_length_2026_cov_3.496195, whole genome shotgun sequence.
ACCESSION   DAOSYE010000420 DAOSYE010000000
VERSION     DAOSYE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45749_length_2026_cov_3.496195"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..478)
                     /locus_tag="PLU43_07610"
     CDS             complement(86..478)
                     /locus_tag="PLU43_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28316.1"
                     /translation="MTREQFCDRLLESYSAYYDIEKTDPTAELPLIAKCSFFVHSEKY
                     VLLKSAKLWSADSKEYVYLFSVSHLTKKLYEKYRNYAYEKGMALIEPKPGHMYTYITA
                     IFVCDTYESAAKSALKRCRLHKNFHFSK"
     gene            complement(606..1520)
                     /locus_tag="PLU43_07615"
     CDS             complement(606..1520)
                     /locus_tag="PLU43_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO28317.1"
                     /translation="MKNYQFTIEEEQQNERIDKCIAAVFGDLSRSYIQKLIKEERVLI
                     NGNICKANARPVCGNLITFSTPDAVLPDILPENIPLDILYEDHDLIIVNKPKGMVVHP
                     APGHYSGTLVNALLYHCGDELSGINGVMRPGIVHRIDMDTTGSVMACKNDAAHAFIAA
                     QLKEHSINRKYVALVHGIMKDDTGRINAPIGRHPRERKKMAVNYKNGREAITNYRVLQ
                     RFKEYTLVECSLETGRTHQIRVHMASIHHPVVGDPVYCSRKNPEKLQGQVLHAKTLGF
                     IHPSLKTYMEIDAPLPAYFERLLDDLVL"
     gene            complement(1526..2023)
                     /gene="lspA"
                     /locus_tag="PLU43_07620"
     CDS             complement(1526..2023)
                     /gene="lspA"
                     /locus_tag="PLU43_07620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO28318.1"
                     /translation="MLVLIDQLTKLQAVRMLKDKPAVPVIPGILELYYLPNGNTGAAF
                     GILQGHKSLFLIISTIVIVLMLYFLYHIPATKRYSHLHILLVCIAAGGAGNMIDRIRL
                     DYVIDFIYISAINFPVFNIADCYVSVSTVLLALLLLFYYKENDLSCLEKEIKKPFRKK
                     NKEGD"
BASE COUNT          600 a          415 c          391 g          620 t
ORIGIN      
        1 gcttgcgatg ccgcccttct gggcgattag tgacaatagc cctttggagg gctaatataa
       61 aaccaccagt tgaactggtg gctgattact tagaaaaatg aaaattttta tgcagacggc
      121 atcttttcaa tgccgattta gccgcagatt cataagtatc acatacaaaa atcgctgtaa
      181 tataagtata catatgtcct ggttttggct cgatcagggc catccctttt tcatatgcat
      241 aattcctgta tttttcataa agttttttgg tcaggtggct gactgaaaaa agatagacat
      301 attctttgct gtcggcactc cataactttg cactcttcaa aagaacgtat ttttcagagt
      361 ggacaaaaaa agaacatttt gcaataagag gtaattcggc tgtaggatct gttttttcga
      421 tatcataata agcgctgtaa ctttccagta atctgtcaca aaactgttct cttgtcatta
      481 tctgttccaa tcaattatga atgtgtaaaa ttgcatgcat ataattctaa aaaaaatcag
      541 caaattctaa aaaaaactat gaatttatta aatttctatg aaattatacc accctttttc
      601 acacattaca atactaaatc gtcaagtaat ctttcaaaat atgcaggtaa tggagcatcg
      661 atctccatat atgttttcag agacggatga ataaatccca atgtttttgc atgcaaaacc
      721 tgtccctgta atttttcggg attttttctg ctgcagtata ccggatcgcc aacaaccgga
      781 tgatggatac tggccatatg gacgcgtatt tgatgagtac ggccggtctc taagctgcac
      841 tctaccaatg tgtattcttt aaatcgctgc agtacccggt agtttgtaat tgcttctctc
      901 ccatttttat aatttacggc catttttttt cgttcacgcg gatgacggcc gatcggcgca
      961 ttgatccttc cggtatcatc tttcatgatc ccatgtacaa gagcaacata ttttctgtta
     1021 atggaatgtt ctttcagctg cgcagcaata aaagcatgtg ccgcatcatt tttgcacgcc
     1081 atgacggaac cggttgtatc catgtcgatt cgatgtacga tgcctggacg catcacgcca
     1141 ttgattcctg ataattcatc gccgcaatga tataacagtg cattgacaag agttccgctg
     1201 taatgtccgg gcgcaggatg aacaaccatt ccctttggtt tattaacaat gatcagatca
     1261 tggtcttcat ataaaatatc cagaggaata ttttccggca gaatatcagg cagaacagcg
     1321 tcaggcgtgg aaaaggtgat cagattgccg cagaccggcc tggcatttgc tttacagata
     1381 tttccgttta taaggacccg ttcttctttt atgagctttt ggatataaga gcgtgaaaga
     1441 tcaccgaata cggctgcaat acacttgtct attcgttcgt tctgctgctc ttcttcaatt
     1501 gtaaactgat aattcttcat aggagtcaat cgccctcttt attcttttta cgaaaaggtt
     1561 ttttgatttc cttttccaga caggacagat cattttcttt ataataaaat aaaagaagca
     1621 gcgcaagcag aaccgttgat acggaaacat aacagtctgc aatattaaat acaggaaaat
     1681 tgattgcact gatataaata aaatcaatga cataatccag tcggatacga tcgatcatat
     1741 tgccggcacc accggctgca atgcagacca gtaatatgtg cagatggctg taacgcttgg
     1801 ttgccggaat atggtataaa aaataaagca tcagaacaat tacgatagtg ctgattatga
     1861 gaaacaggga tttatgtccc tgcaggattc caaaagccgc accggtattt ccattcggca
     1921 gataataaag ctccaaaatg cctggaatga ctggaaccgc aggcttgtcc ttcagcatac
     1981 ggactgcctg cagttttgta agctgatcga tcagcacaag tataca
//
LOCUS       DAOSYE010000421         2025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45784_length_2025_cov_3.722843, whole genome shotgun sequence.
ACCESSION   DAOSYE010000421 DAOSYE010000000
VERSION     DAOSYE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45784_length_2025_cov_3.722843"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PLU43_07625"
     CDS             complement(<1..955)
                     /locus_tag="PLU43_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOO28319.1"
                     /translation="MKDKRKRNKRKALQIKIPKVKPAPIKFNKLNNKLPNSISLKLLG
                     VFGITMFFMLLLMIVFFAKALGYNQEYQQVLENVNKINYIKTEISEQPNRMMSLCMMQ
                     NNVGESGEVEIVKTMLADLDEISASIGDNAAFQGNQGMIVSIKTPLTDYQTILTEIVD
                     AGTNGCFPPLDTDTTAKVQGLFGLNASIANYCGSLITMEVERSEVVQKEVNQNFRRTI
                     IIVGILFLLLLSVGMALCTIVVKSITRRIHRLKNEISIVAQGDLSREDIVIGSNDEIK
                     ELAVAFNHMSGSLRKIIGKMAQVTDEMDRSTQIVSSSAESNS"
     gene            complement(975..1415)
                     /locus_tag="PLU43_07630"
     CDS             complement(975..1415)
                     /locus_tag="PLU43_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOO28320.1"
                     /translation="MLKGIPKILSPELLKVLCEMGHSDRLVISDGNFPAESMGKNAIV
                     IRCDGHGVPELLDAILQVFPLDTYVEHPVNLMEVMKGDPVETPIWDDYKKIVGKYDGR
                     GENAVGNIERFSFYEEAKTAYAIIATGESALYANIMLQKGVVTD"
     gene            complement(1531..>2025)
                     /locus_tag="PLU43_07635"
     CDS             complement(1531..>2025)
                     /locus_tag="PLU43_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28321.1"
                     /translation="LVIADGKTVNVEGNDKFVDGCQIEKTDGADVVIEVLVPAQVTSE
                     LSAIDCQTLLNKDDTIAIYGSNQHSGEAMVTANENLNKFGTGDDQVIGVAFDSGAVIK
                     GAVKDGTFLGAITQAPVAMGEALVQALYDAASGKEVSDVDTGCQWYTADNMDDEEIAQ
                     NLYD"
BASE COUNT          533 a          518 c          354 g          620 t
ORIGIN      
        1 tcgaattgct ctcggcactg gaacttacga tctgcgtgga gcggtccatt tcatctgtga
       61 cctgagccat tttcccgatg atctttctta agctgccgct catatggtta aaggctaccg
      121 caagttcttt tatttcatca ttggaaccga tcacgatatc ttcccgtgac agatctccct
      181 gcgcaacgat cgaaatttca tttttcagcc gatggatcct tcttgtgatc gattttacaa
      241 cgatcgtaca taatgccatg ccaacggaca ataaaagcag gaaaagaatt ccgacaatga
      301 taatcgtacg tctgaaattc tggttgacct ccttttgtac gacctcactg cgttccactt
      361 ccatagtgat cagactgccg cagtaatttg caatggacgc attcagaccg aataatccct
      421 gtacttttgc cgtagtatcc gtatccagcg gcggaaaaca cccgttcgtt cccgcatcta
      481 cgatttctgt cagtattgtc tggtaatccg tcagcggcgt tttgatcgat acgatcattc
      541 cctgatttcc ctgaaaagcc gcattatcgc ctatacttgc cgaaatttca tcaagatccg
      601 caagcatcgt tttcacgatt tcaacttctc ctgactcccc cacattattc tgcatcatac
      661 aaaggctcat catacgattg ggctgttcgc tgatctcggt tttaatgtag ttgatcttat
      721 tgacattttc cagcacctgc tgatattcct ggttataacc aagtgctttc gcaaaaaata
      781 caatcatcag cagcagcata aaaaacatcg taatcccgaa tacacccagc agctttaatg
      841 atatactgtt aggcagctta ttatttaatt tgttaaattt gattggggcc ggtttcacct
      901 ttgggatctt tatttgcaaa gcctttcgtt tattcctttt ccttttatcc ttcataactt
      961 ctccaatcaa agcattaatc cgttacaacg cctttctgca gcataatatt tgcatagagc
     1021 gccgattcac cagttgcaat aatggcatac gcagttttgg cttcctcata aaaagaaaaa
     1081 cgttcgatat tgccgactgc attttcgcct ctgccatcat atttccctac aattttctta
     1141 tagtcgtccc aaatcggagt ctcaacagga tctcctttca tgacctccat cagatttaca
     1201 ggatgctcca cataggtgtc gagcgggaac acctgcagaa tcgcatccag aagttccggt
     1261 actccatgtc cgtcacaacg gataacgatc gcattctttc ccattgattc agccggaaaa
     1321 ttaccgtctg aaatcacaag cctgtcactg tgccccattt cgcacagaac ttttaataac
     1381 tccggagata aaattttagg aattcctttt agcatgacag tttcctccat tcagccataa
     1441 attctcattt ccataatata acaccattcg ggcataccat aagctatgat atgcccgcct
     1501 gatttttata cggtttttta aatattcaga ttagtcgtaa aggttctgag cgatttcctc
     1561 atcgtccata ttgtctgccg tataccactg gcatcctgta tcaacatcgg aaacttcttt
     1621 tccgcttgca gcatcataaa gagcctgaac aagcgcttca cccatagcta ccggagcctg
     1681 tgtaatagca ccaaggaatg tgccgtcctt tacagcgcct ttgataacag cacctgagtc
     1741 aaatgctacg ccgattacct gatcatcacc agtaccgaac ttattaaggt tttcatttgc
     1801 cgtaaccata gcttcacctg aatgctgatt ggaaccataa atagcgatcg tatcatcttt
     1861 attaagaagt gtctggcagt caatggctga aagctctgaa gtaacctgtg caggtactaa
     1921 tacttcaata acaacatcag cgccgtctgt cttttcgatc tggcagccgt ctacaaactt
     1981 atcattgcct tctacattta cagtcttgcc gtcagcaatt acaag
//
LOCUS       DAOSYE010000422         2025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_45791_length_2025_cov_3.186294, whole genome shotgun sequence.
ACCESSION   DAOSYE010000422 DAOSYE010000000
VERSION     DAOSYE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45791_length_2025_cov_3.186294"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /gene="metE"
                     /locus_tag="PLU43_07640"
     CDS             complement(<1..1824)
                     /gene="metE"
                     /locus_tag="PLU43_07640"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOO28322.1"
                     /translation="MKSSVIAFPRIGEKRELKFASEKYFAGEITEEELLSTAADLRLA
                     HWKLMKKEGIEWIPSGDFSLYDNMLDTAVLLHAVPEEYRKLGLHATAAYFAMARGYQG
                     EKGDVKALAMKKWFNTNYHYIVPELDDTTEIAVTGNKLFHEFTEAKEAGIETKPTMIG
                     PFTFLKLAYFRGKKSAADFVPQITAAYGSVLSRLGQLGADWAAFEEPALVKDLCREDV
                     ELFQTLYKEILKEKGNVKVLLQTYFGDIRDCYREAVKLPFDGLGLDFVEGEKTEELLA
                     QYGFPQDKVLFAGVVNGKNIWKCSYEKTCLLIEKIRNYGSETVLQPSCSLLLVPYTLA
                     YEKKLPEDVKQYFSFALEKLAELSDLKQLAENASWRTDPVFLANREIFYKPRTGEDRE
                     VREAIKKLTDSDFRREMLRCEREKLQKEKLGLPLFPTTTIGSFPQTKLVKANRSAFRR
                     GEKTKEQYDEQIRELMKDCIKRQEETGLDVLVHGEFERNDMVEYFGENLKGYVFTEFG
                     WVQSYGTRCLKPPIIFGDIKREHPITVAYIKYAQSLTKKPVKGMLTGPVTILNWSFPR
                     EDISLKEMAYQIGLAIRGEVTDLEKAGISVEELAESIARRGL"
BASE COUNT          474 a          553 c          370 g          628 t
ORIGIN      
        1 caggccgcgc cgggcgatgg actcagccag ttcctcgacc gaaataccgg ccttttccag
       61 atccgtaact tccccgcgga tcgcaagtcc gatctggtac gccatctctt tcagggaaat
      121 atcttctctt ggaaaggacc agtttaaaat cgtgaccggc ccggtaagca ttcccttgac
      181 aggctttttt gtcagactct gcgcatactt gatatatgca accgtgatcg gatgttcccg
      241 tttgatatct ccgaaaataa tcggcggttt cagacatctc gtcccgtatg actgtaccca
      301 cccaaattcc gtaaatacat agcctttcag attttcaccg aaatactcta ccatatcgtt
      361 tcgctcaaac tctccgtgca ctaatacatc cagacccgtc tcttcctgcc gctttatgca
      421 gtctttcatc aattcccgga tctgttcatc atactgttct tttgtttttt ctccccggcg
      481 gaaagcactc ctgtttgcct ttaccagttt cgtctgcgga aaagagccga tcgtcgttgt
      541 tggaaacaaa ggaagtccca gtttttcctt ttgcagtttt tctctttcac accttagcat
      601 ttcccgcctg aaatcactgt cggtcagttt ttttatggct tctcttactt ccctgtcttc
      661 accggtacgg ggcttataaa agatctcccg atttgccaga aataccggat cggttctcca
      721 tgatgcattc tccgcaagct gcttcagatc tgaaagctct gcaagctttt cgagcgcaaa
      781 cgaaaaatac tgcttcacgt cttccggaag ttttttctca taagcaagcg tatatggaac
      841 aagcaataac gaacaggagg gctgcaatac tgtttcactc ccataattcc tgattttttc
      901 aatcagcaga caggtttttt catagctgca tttccatatg ttttttccgt tgacgactcc
      961 ggcaaacagc actttgtcct gcggaaatcc atactgtgct aacagctcct ctgttttttc
     1021 accttccacg aagtcaagac ccagtccgtc aaagggcagc ttcacggctt ccctgtagca
     1081 gtcgcggatg tctccgaaat atgtctgaag cagtaccttc acattcccct tctctttcag
     1141 gatctcttta taaagcgttt gaaacagttc cacatcttcc ctgcaaagat cttttacaag
     1201 cgccggttcc tcaaaagctg cccagtcggc cccaagctgt ccgagcctgg aaagaacact
     1261 gccataagct gcagtgatct gcggaacaaa gtctgcggcc gactttttcc cgcgaaaata
     1321 cgcaagcttc aaaaacgtaa acggcccgat cattgtcggc tttgtctcta ttccggcttc
     1381 ctttgcctcc gtaaactcat ggaacagctt gttccctgtt actgcaatct ccgtcgtatc
     1441 gtcaagctcc ggcacgatat aatgatagtt cgtattaaac cactttttca ttgccagtgc
     1501 tttcacatcg cctttttctc cctgatatcc tcttgccatc gcaaaatacg cagccgtcgc
     1561 atgcagtccc agcttccggt attcttccgg aaccgcatgc agcagcacag ccgtatcgag
     1621 catgttgtca taaagggaaa aatccccgga tgggatccat tcgatcccct cttttttcat
     1681 aagcttccaa tgcgcaagac gcagatctgc tgcagtggaa agcagttcct cttctgtaat
     1741 ttcacctgca aaatattttt ccgatgcaaa ttttaactct cttttttccc cgattctcgg
     1801 aaatgcaatt actgaacttt tcatttttcg atctccttat gttgttttga tgtatccaaa
     1861 ccggatgttt tatgttaaga ataaatctta cataggaatc attcttattt caactttaaa
     1921 attttttacc tgttcctgca ttggtcacat acaccctcga gaatcacttt aattgttgat
     1981 accgtatatc gatccctgat tattttcgga atcttcagtt tgtca
//
LOCUS       DAOSYE010000423         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46022_length_2019_cov_3.147149, whole genome shotgun sequence.
ACCESSION   DAOSYE010000423 DAOSYE010000000
VERSION     DAOSYE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46022_length_2019_cov_3.147149"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PLU43_07645"
     CDS             complement(<1..1301)
                     /locus_tag="PLU43_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28323.1"
                     /translation="MAGNSLKQLFRTPLRTLLFFLLIVVTCIAISVGSVLVIENRRAL
                     KTYENNFVTIGTVEQQAAGIVQTEQWNADLSDYQVFQKPEYNQFLPLSVISDLEFDFI
                     QEPERRPFYYSDASEYELPDYGTVNRSGFVEFTPLEDCIPDHRVKVHLTKKFWGLEGI
                     MEGTDIEIYARYTKDPAPLYADKTYVALIGEDLTVSDTGEFITDLVPEALSIEQYDAA
                     GQLLETDFAEDILYYEVTEDFYQTQEGKMLLQYEAGVERDRQAIPVVGTDQTILLMPF
                     YKKDAYLCEGREISAEEYAAGEQVCLVTKDFANANDLTIGDPVHVQLYAADYKNAVGR
                     EFLWKGGQSWSNSPVNAKGKLYPVFYDASYRIVGIYDLTVGADTNYYYAMGMNEVIVP
                     SKSISCSDENNIMDYGPMMGYNTSFQIANGQTEEFMEEWEKT"
     gene            complement(1306..1899)
                     /locus_tag="PLU43_07650"
     CDS             complement(1306..1899)
                     /locus_tag="PLU43_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28324.1"
                     /translation="MKNSFETGKMYAITGKSGSGKSTLLSLLAGLDTPAAGEIFIEGQ
                     SLKNIDRDEYRRKQASVVYQAFHLFPLLNAVENVMFPMELNGVKQTEAKLQAEELIKK
                     VGLEAKIFRQFPKMMSGGEQQRIAIARAMASKGTILLADEPTGNLDSKNEELITALLK
                     KLAYEEQYTVIVVTHNAAVSKKADIVYVMQDGELKGE"
BASE COUNT          480 a          576 c          383 g          580 t
ORIGIN      
        1 gttttctccc attcctccat aaattcctct gtctggccgt tcgctatctg aaaggaagta
       61 ttatatccca tcatcgggcc ataatccatg atattgttct catctgaaca actgatcgac
      121 tttgacggca cgatcacttc gttcattccc attgcataat aatagttcgt atccgcaccg
      181 actgtcagat catagatccc gacgatccgg taactggcat catagaagac cggatacagt
      241 tttccctttg catttaccgg actgttggac cagctctgac cgcccttcca taaaaattct
      301 ctgcccacgg catttttata gtctgccgca taaagctgca catgtacggg gtccccgatc
      361 gtcagatcat ttgcattggc aaaatctttc gtgaccaggc atacctgctc ccctgcagca
      421 tactcctccg cactgatctc tctgccctca catagatacg catccttttt ataaaacggc
      481 atgagcagga tcgtctgatc tgtcccgaca acaggaattg cctgtctgtc tctttccact
      541 ccagcctcat actgcagaag cattttccct tcctgcgtct gataaaaatc ctccgttact
      601 tcataataca gaatatcctc cgcaaagtct gtttcaagaa gctgacccgc cgcatcatac
      661 tgctcgatag acagcgcctc cggcaccaga tccgttatga attctcccgt atcactcact
      721 gtcagatcct ctccgatcag cgcaacataa gtcttgtctg catacagggg ggcaggatcc
      781 tttgtatagc gtgcgtagat ctcaatatcc gtgccttcca taatgccctc aagtccccag
      841 aatttttttg tcagatggac cttgacccgg tggtccggga tacagtcctc taacggggta
      901 aactctacaa atccgctcct gttcacagtc ccatagtccg gcagttcata ttccgacgcg
      961 tcggaataat aaaacggccg tcgttccggc tcctgtataa agtcaaactc cagatcagag
     1021 ataacggata atggcagaaa ctgattatac tcaggctttt gaaaaacctg atagtcagac
     1081 agatccgcat tccactgctc tgtctgaacg attccggccg cctgctgttc tacggttccg
     1141 atcgtaacaa aattattttc ataggttttc agagcccttc tgttttctat cactaaaaca
     1201 ctgccgacag aaatggcaat gcacgtcacc acgatcagca aaaagaacaa aagcgtccga
     1261 agcggcgttc tgaaaagctg tttaagactg tttcccgcca tccttctatt cccccttcag
     1321 ttctccgtcc tgcatcacat agacaatatc cgctttcttt gagaccgcgg cgttatgcgt
     1381 aactacaata accgtgtact gttcctcata tgccagcttc ttaagaagcg ccgtgatcag
     1441 ttcttcgttt tttgagtcca gattgcctgt cggttcgtcc gcaagcagga tcgttccttt
     1501 ggatgccatt gctcgtgcga tggcgatccg ctgctgttct cctccgctca tcattttagg
     1561 gaactgccga aagatcttcg cttccagtcc gacctttttg ataagttcct ctgcctgcag
     1621 ctttgcttcc gtttgtttta caccattcag ctccattggg aacatgacat tttccaccgc
     1681 attcaacaag gggaacaaat gaaacgcctg ataaacgacc gatgcctgtt ttctcctgta
     1741 ttcatcacgg tcaatatttt tcaggctctg cccctcaata aagatctctc ctgctgccgg
     1801 cgtatccaga cctgcaagca gcgataataa cgtacttttt ccgctgccgg atttccctgt
     1861 aattgcgtac atttttcctg tttcaaaaga attcttaata aatcaagagc caaggccgtt
     1921 tgctctggcg ctaacctaac tgctgtttca agctctttct ccgccctaag ataatctgac
     1981 tttctttcat agacgcggcc taagtaatag tgcccgacc
//
LOCUS       DAOSYE010000424         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46068_length_2018_cov_2.725930, whole genome shotgun sequence.
ACCESSION   DAOSYE010000424 DAOSYE010000000
VERSION     DAOSYE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46068_length_2018_cov_2.725930"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLU43_07655"
     CDS             complement(<1..1619)
                     /locus_tag="PLU43_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO28325.1"
                     /translation="MNTAEQLIEFDRIKEQLAEFSCTQKGKQAAMQLKPYLSETEVRT
                     GQRETSEARAMFEKCGNPPVTALEGIEEWIGAAQRGECLSPWQLEQIRAILNAVGRMK
                     DYLNHCKQYQISLAYYEENLDSMEELRLEISEKIRGERVDDFASRLLKTLREEIDAAD
                     AKMHEKADAAIRANKACMSDSFSTIRSGHLCIPVKKEYKYKIKGAVIDKSATGNTMFI
                     EPLAVSKCYEKLQELKIEEENEERRILYTLSAMLSDKEELFLKNSRTIEKLDFIFAKA
                     RLSLEYDGVEPLFNTLRYIRLKGGRHPLMDKKTSVPLDFEIGNKINGIVITGPNTGGK
                     TVALKTVMINCMMAQSGLHVPCEKAEICMNDAFLCDIGDGQNLSENLSTFSAHITNVL
                     SILRRADRESLVVMDELGSGTDPAEGMGIAIAVLEELKKSGALFLVTTHYPEVKEYTK
                     KEEHIVNARMTFDKETLRPLYRLVIGEAGESCAFYIAGKLGMPKDMLRRAAYEAYGNE
                     PAVDVDRLAEKERLEKTSHEKIKRKKDISRQ"
BASE COUNT          475 a          544 c          350 g          649 t
ORIGIN      
        1 tcctgccggg atatgtcttt tttccttttg atcttttcat gagacgtctt ttcaagccgc
       61 tccttctctg ccagcctgtc aacatcgacc gccggttcgt ttccgtacgc ctcataagcc
      121 gcacgccgca gcatatcctt tggcatgccg agcttcccgg caatataaaa cgcgcagctc
      181 tctcctgctt ctccgatcac aagccggtaa agaggacgca gtgtttcctt atcaaaggtc
      241 atccgtgcat tcacaatatg ctcttctttt tttgtatatt cctttacttc cggatagtgc
      301 gttgttacaa gaaacaaggc accgcttttt ttcaattctt ccagaaccgc aatggcaatt
      361 cccatccctt ccgcaggatc ggttcctgaa ccgagttcat ccattacaac aagactttcc
      421 ctgtccgccc tacgcagaat actgagcaca tttgtaatat gtgctgaaaa agtcgacaaa
      481 ttttcagaca gattctgtcc gtccccgata tcacataaaa acgcatcatt catacagatc
      541 tctgcctttt cacatggaac atgcagaccg ctctgagcca tcatgcagtt gatcatcact
      601 gttttaagcg ccactgtctt tccgcccgta ttcggcccgg tgatcacaat accatttatt
      661 ttgtttccga tctcaaaatc aagcggtaca cttgtctttt tatccatcag cggatgcctt
      721 ccgcctttta atctgatata ccgcaacgta ttaaaaagcg gttccactcc gtcatattcc
      781 agactgagcc tggcttttgc aaaaataaaa tccagctttt cgatcgtccg gctgttcttc
      841 aaaaacaatt cttctttatc agacagcatg gctgacagtg tatataaaat tctgcgctcc
      901 tcattttctt cctctatttt tagttcctgc agcttttcat aacactttga tacagccagc
      961 ggctctatga acattgtatt tccggttgcg gatttatcaa tgaccgcgcc tttgatctta
     1021 tatttgtatt ccttttttac aggaatgcag agatgcccgc tgcgtatcgt actgaagctg
     1081 tccgacatgc atgctttgtt tgcccgaatg gctgcatccg ctttttcatg cattttagcg
     1141 tctgctgcat cgatttcttc ccgcagcgtc ttcagcagtc tggaagcaaa atcatctacc
     1201 cgttcgccgc ggatcttttc actgatttct agacgaagct cctccatgga atccagattt
     1261 tcctcataat atgccaggct gatctgatat tgcttacagt ggttcagata gtctttcatc
     1321 cggccgaccg cattcagtat tgcacggatc tgctccagct gccatggaga gagacattct
     1381 cccctctgcg cggcgccgat ccattcctcg atcccttcca gtgccgttac cggcggattt
     1441 ccgcattttt caaacatggc tctggcttcc gacgtctctc tctgtcctgt tctcacttct
     1501 gtttccgaca aatatggttt cagctgcatt gccgcctgct ttcctttttg tgtacaggaa
     1561 aactcagcca gctgttcttt tattctatca aattctatta attgttccgc tgtattcatt
     1621 ttttaaccga tccggccgac aagcaccgga ttttcctttc tctttatatt acaaaacacc
     1681 gcaggatgct gcctttacag cacaaattgc ggtgtttttc ctgatttatt ttagattctg
     1741 aacaggcgcg gcgcaggatg caaaaagagc atgaccatac agtcatgctc gaaaatccga
     1801 tcctattctc tatacgcgtg catttgtaag gcaaacaaaa agacatgacc atctaactta
     1861 ggacagccat ctcatcaact atttaattcg ttgaattttc cattactacc acgcttaaca
     1921 tccaatacct cataaatctt tcttgtctct gttttctaaa attataattt cttttttcgt
     1981 ttctgtcaac aaacagttta caaaacaaaa aagaaaat
//
LOCUS       DAOSYE010000425         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46218_length_2014_cov_3.584482, whole genome shotgun sequence.
ACCESSION   DAOSYE010000425 DAOSYE010000000
VERSION     DAOSYE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46218_length_2014_cov_3.584482"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLU43_07660"
     CDS             complement(<1..924)
                     /locus_tag="PLU43_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28326.1"
                     /translation="MSTVNSDTITLFYCKTLTDNGMTDYVLSGYNVIPAYFHTKKIGT
                     IGTPIWDFFKLGTWGTEKIRQQLASGCKNIPVGDISFESQKYHCELICMPNENLTLQL
                     SKLEPFSLSDLVYFKLMVENSIEAMALLENRNGTFFYLYNNAQHQKITGFSAVESSEK
                     NICDILGDPYYKKYETNCRHCIKTNSPILFKGLANWNGTVKERLCRLVPIFYKNKEYI
                     MAIEIETDSLKNWMRENQLALTKFDNMFHDHASIMLLIEPETGKITHILQLEQADATQ
                     LVQTLQQVIQDGARGSRGGQSTSSIRMTADAG"
     gene            1263..>2014
                     /locus_tag="PLU43_07665"
     CDS             1263..>2014
                     /locus_tag="PLU43_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOO28327.1"
                     /translation="MIIDPKKMKHVELTGEGLTVEKVVAVARFQAEVSISKEAVEKMR
                     KSRARVEQIVESEVPTYGINTGFGDFSTISIDKGNIKNIQKNLILSHCVATGKPYEKE
                     VVLAIMLLRANALCIGFSGIRPEVVQTLVKMINQKVIPVIPQKGSLGASGDLAPLAHM
                     VLPLIGYGKAVYEGEELDGADAMKRAGIPLVELEAKEGLALINGTQAMTAVGTLAYYD
                     ALMAAQLADIACAMSMEALLALTNAFDERVQAV"
BASE COUNT          608 a          367 c          491 g          548 t
ORIGIN      
        1 gcctgcatcg gcagtcatac gaatgctgga cgtagactgg ccaccgcgtg aaccgcgtgc
       61 gccgtcttga atcacttgct gaagcgtctg cacgagttgc gtcgcatccg cctgttcgag
      121 ttgaaggatg tgcgtgatct tcccggtttc aggctcgatc agcagcataa tggaagcgtg
      181 atcgtgaaac atgttatcga atttggtcaa tgcaagctga ttttcccgca tccagttttt
      241 cagggaatcg gtttcgatct ctatggccat gatgtattct ttatttttat aaaagatcgg
      301 aaccagtctg cacagacgtt ctttaacagt tccgttccag ttggccaatc ctttgaacag
      361 gatcggagaa ttggttttaa tgcagtgcct gcaattcgtt tcatattttt tataataggg
      421 gtcccctaaa atgtcacata tatttttttc ggagctttcc acagccgaaa atccggttat
      481 tttctggtgc tgtgcattat tgtaaaggta aaaaaaggtc ccgtttctgt tctccagtaa
      541 agccattgct tcaatactgt tttcgaccat cagcttaaag taaacaagat cagacaaaga
      601 aaacggttcg agcttactca gctgaagcgt cagattttca ttaggcatac agatcagctc
      661 gcaatgatat ttttgagact caaagctgat gtcgccaaca ggaatgtttt tgcagccgga
      721 ggcaagctgc tgtctgatct tttccgtacc ccatgttcct aatttgaaaa aatcccagat
      781 aggggtacca atagttccta tttttttcgt atgaaagtat gcaggaatta cattgtagcc
      841 ggaaagtaca taatctgtca tgccgttatc cgtcaatgtt ttgcaataaa aaagtgtgat
      901 agtatcggaa tttacagtac tcatagacag atacctcctt gtgagtgact tgacacgctg
      961 tatatgtatt acttaaccat agaaacagtc ggattgtcaa ggagtccggt tacaataaat
     1021 ggataagcag gcaaaggggc ttaaatagca gttgacagga aattatttta ggcggataat
     1081 ataaaaaagt tagcttataa gttaaaatta ttaatcagac ctgataagaa gaaaatttat
     1141 gaaaagacga atacaaataa tggaaaagaa taataaaaaa agcagacatt ggtgtctgct
     1201 ttttttaatg ctttaatgcg ataaaggaat aaaacgataa aagatctatg gaggcggaaa
     1261 caatgataat cgatccgaaa aaaatgaaac atgtggaatt aacaggggaa gggcttactg
     1321 tagaaaaagt agtggcagtt gccagatttc aggcagaagt aagcatttca aaagaagcgg
     1381 tggaaaaaat gcgaaaatcc cgtgccaggg tagaacagat cgtggaaagt gaagtgccga
     1441 cttacggtat taataccgga tttggggatt ttagtacgat ttccattgat aaaggcaata
     1501 ttaaaaatat acagaaaaat ctgatattga gccattgtgt ggcaacggga aagccatatg
     1561 aaaaggaagt ggttctcgcg atcatgctgc ttcgcgcaaa tgcactctgt atcgggtttt
     1621 ccgggattcg gccggaagtg gtgcagacgc ttgtaaaaat gatcaatcaa aaggtgatcc
     1681 ctgtgattcc gcagaagggt tcgcttggag ccagcgggga tctggcgccg ctggcccata
     1741 tggtactgcc tttgatcgga tatggaaagg ctgtgtatga aggagaggag ttggacggag
     1801 cagatgccat gaaacgtgca gggattcctt tagtggaact ggaggcaaag gaaggactgg
     1861 cacttattaa tggaacacag gcaatgacgg cagtcggaac acttgcctat tatgatgcac
     1921 ttatggcagc ccagcttgcg gacattgcct gtgcaatgag catggaagca cttttggcat
     1981 tgacgaatgc ctttgatgaa agggtacagg cagt
//
LOCUS       DAOSYE010000426         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46227_length_2014_cov_3.182746, whole genome shotgun sequence.
ACCESSION   DAOSYE010000426 DAOSYE010000000
VERSION     DAOSYE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46227_length_2014_cov_3.182746"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLU43_07670"
     CDS             <1..1274
                     /locus_tag="PLU43_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOO28328.1"
                     /translation="CGLWTEWGTLQKADLVKKQAVRLEADKKSAILKEMEDGDTVTVL
                     EQSEDWSEVQTQDLLTGYIETKYLTNIYEETQTAVNDVAELECSSISKDYPIVLAWDM
                     VTNVDANSLLSDRLQSVSGLTTLSPTWFSLADDEGTVASIASQSYVDTAHTAGYEVWG
                     LVDNLTYPEVSTYNILSDSEKRAYVIEQLISYAGQYGLDGINVDFESLSEEAGEPFIQ
                     FIRELSIQCRKEGLVLSVDNYVPAAYNDFYNRKEQGVFADYVIIMGYDEHYAGSTESG
                     SVASIDYVRTGIEKTVAEVPAEKVINALPLYTRIWIETPKTDEEIAAEDTNTEYIPYN
                     LNVQTVGMDDALNAVKNAGAETTWDETTAQNYAEWTKEDSTYKVWLEDTQSLTAKLQV
                     MENNQLAGVAVWQLGYADSSVWDVIAQYYAQ"
     gene            complement(1302..>2014)
                     /locus_tag="PLU43_07675"
     CDS             complement(1302..>2014)
                     /locus_tag="PLU43_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28329.1"
                     /translation="EKPVRVAITALGNWKKYKIAQLHYATNGREGLAAMRELRPDLVF
                     VDMQMPVINGIDFLKQATQEFEEVKYIVVSGYDDFQYAQAAIKNGAIDYLLKPLSSED
                     LNTAIERAFIQLGGILDEPDGDSAAQQLTPYEVIEIIRDYVKKNYACDISISMFSEKY
                     FFSKEYLSKLFKKQYHIGIYEYALKLRMDRAKELLSDSSIQIQEISDRLGYSNNNYFS
                     KAFKNYCGISPTDYRDSL"
BASE COUNT          541 a          388 c          533 g          552 t
ORIGIN      
        1 gctgcgggct ttggaccgaa tggggaactt tgcagaaagc ggatcttgta aaaaaacagg
       61 cggttcgtct ggaagcggat aaaaagagtg caatacttaa agagatggag gacggagata
      121 ctgttaccgt gctggaacag tcggaagact ggtcggaagt acagacacag gatctgctga
      181 ccggctatat agaaacgaaa tatttgacaa atatttacga ggaaacgcag actgcggtca
      241 atgatgtggc agaattggaa tgttccagta tatccaagga ttatccgatc gtactcgcat
      301 gggatatggt aacgaatgtg gatgcaaaca gtctgctttc ggatcgcctg cagtcggtct
      361 ccggtctgac gacactctct ccgacatggt tttcgcttgc ggatgatgag ggaacagttg
      421 cctcgattgc gagccagtct tatgtggata cagcacatac ggcaggctat gaagtctggg
      481 gtcttgtcga taatctgact tatccggaag tcagtaccta taatatttta tcggattctg
      541 agaaacgggc gtatgtgatc gaacagctga tcagctatgc aggacagtat ggactggatg
      601 gtatcaatgt ggattttgaa tccttgtccg aggaggcagg agagcctttt attcagttta
      661 tcagggagct ttcgatccag tgcagaaaag agggccttgt actttccgta gataattatg
      721 ttccggcggc atataatgac ttttataacc gaaaagagca gggtgtattt gcggactatg
      781 tgattatcat gggttatgat gaacattatg ccggatccac agaaagcgga tccgttgctt
      841 ctattgatta tgtaagaacc ggtattgaaa aaacagtcgc ggaagtgccg gctgaaaaag
      901 ttatcaatgc actgccgctt tatacgagga tctggataga aacgccgaag acagatgagg
      961 aaattgcggc agaagataca aatacggaat atattccata taatctgaat gtgcagactg
     1021 tcggaatgga tgatgcgctt aatgcggtaa agaacgcagg cgctgaaacg acatgggatg
     1081 agacaaccgc acaaaattat gcggagtgga cgaaagaaga ttctacttac aaggtctggc
     1141 tggaagacac acagtccctt acggcgaagc tgcaggttat ggagaataat cagcttgccg
     1201 gcgtggcagt ctggcagctt ggttatgcgg acagctcagt atgggatgtt attgcgcaat
     1261 attatgcgca ataagtctgt tctgcgctat ggaacggggt gtcacaggct gtcccggtaa
     1321 tcggtcggag agatgccgca gtagttttta aatgccttgc tgaaatagtt gttgttgctg
     1381 tagccaagcc ggtctgatat ttcctgtatc tgaatggaac tgtcggaaag cagttctttt
     1441 gcccggtcca ttcgcagttt cagggcatat tcataaatgc cgatatggta ttgttttttg
     1501 aagagcttgg aaagatattc cttggagaaa aaatattttt cactgaacat ggagatggaa
     1561 atatcacagg cataattttt tttgacgtaa tcgcggataa tttcgattac ttcatagggc
     1621 gtcagctgct gtgctgcgct gtcgccgtcc ggctcgtcaa gtatgccgcc gagctgaata
     1681 aaagcacgtt caatggcagt gtttaaatct tcggagctaa gtggctttaa caaatagtcg
     1741 atagctccgt tttttattgc ggcctgtgca tactgaaaat cgtcgtagcc gcttacgaca
     1801 atatatttga cttcctcaaa ttcctgtgtt gcctgtttta aaaagtcgat accgtttatg
     1861 acgggcatct gcatatctac aaaaacaaga tccggacgca gttctctcat ggcggcaagt
     1921 ccttccctgc cgtttgtagc atagtgcagc tgtgcgattt tgtatttttt ccagttgccg
     1981 agtgcagtga tcgcaactct taccggtttt tcgt
//
LOCUS       DAOSYE010000427         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46234_length_2014_cov_2.807555, whole genome shotgun sequence.
ACCESSION   DAOSYE010000427 DAOSYE010000000
VERSION     DAOSYE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46234_length_2014_cov_2.807555"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..186)
                     /locus_tag="PLU43_07680"
     CDS             complement(49..186)
                     /locus_tag="PLU43_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28330.1"
                     /translation="MKDAHMQNADQAAKVTKFMLDFMFYSGILLCLALPAVMKILSAY
                     Y"
     gene            complement(336..1478)
                     /locus_tag="PLU43_07685"
     CDS             complement(336..1478)
                     /locus_tag="PLU43_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HOO28331.1"
                     /translation="MIEKSREYRNVFLENGYAKEDIRRKVENTFMEIFYGKEKFFHLT
                     EDETMGYMEDTGNHDVRTEGMSYGMMVCVQLDRKKEFDCLWKWAKRYMYMDQGRNAGY
                     FAWSVSPEGVKNSYGPAPDGEEYFAMALFFASHRWGDGEGIFQYSLEAKKLLYSCVHK
                     GEDGIGRAMWDPQNYLIRFIPECDFTDPSYHLPHFYELFSRWAWEIDRPFWKKAAAAS
                     REFLRKACDPVTGLSAEYSTFDGIPYTGDQELFGRHDWYYSDAYRTIANIALDYEWFS
                     KETEYGVWCEGTAEKLQKFFCETAGRDTRAVYTTDGRVLAKKALHPVAITAVNAQASL
                     AAGKDFAKQCVEEFWNTPLRSGARRYYDNFLYLFSMLALSGSYRMW"
     gene            complement(1527..>2014)
                     /locus_tag="PLU43_07690"
     CDS             complement(1527..>2014)
                     /locus_tag="PLU43_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28332.1"
                     /translation="NLGLTNSFWVYVIPGMISAFNMLVIRTYMNGIPDSLEESAQLDG
                     AGYTTIFVKIISPLCKPVYATIALFVAVYQWNSWFDAMLYNRMSDNLTTLQYELMKLL
                     SSVTNQSTSAEVMKNSSGSVTPTSVRAAATIITMLPIVCIYPFLQRYFVTGLTLGGVK
                     E"
BASE COUNT          533 a          550 c          357 g          574 t
ORIGIN      
        1 tatttatcaa tgctttgagg gtggtttata aagtactgac gtagaatctc agtaatatgc
       61 ggacagtatt ttcattacgg ccggcagagc cagacaaagc aatataccgg aataaaacat
      121 aaaatcaagc atgaacttcg ttacttttgc cgcctggtcc gcattttgca tatgcgcatc
      181 tttcatatcc gtttcctccc attttatcct ctttcgcaca taacaacaga ggtgtatttc
      241 tgatgagtct catcatacac ccctgaaaac tgtttgtcaa tatttattta ttgtttttcg
      301 ataatttatt tttgtttatc actaaattta gagaattacc acattctgta actgccgctc
      361 agtgcgagca tggaaaacaa ataaagaaaa ttgtcatagt accgtcttgc gccgctgcga
      421 agcggtgtat tccagaattc ttccacacat tgcttcgcaa agtctttgcc ggccgcaagg
      481 gacgcctgcg catttactgc agtgatcgca accggatgca gcgctttttt cgccagtacc
      541 cgaccgtcag tcgtatacac tgcccgtgtg tctctgccgg cggtctcaca aaaaaacttt
      601 tgaagttttt ccgctgttcc ctcacaccag accccatatt ccgtctcctt tgaaaaccac
      661 tcataatcaa gcgcaatatt ggcaatcgta cggtatgcat cactgtaata ccagtcatga
      721 cgtccgaaaa gctcttgatc tcccgtatac gggatcccgt caaaagtact gtattcagca
      781 ctcagcccgg taaccgggtc gcaggctttc cgcaggaatt ccctgctcgc tgccgctgct
      841 ttcttccaga acggacggtc gatctcccat gcccacctgg aaaataactc ataaaaatgc
      901 ggcagatgat aagacgggtc cgtaaaatca cattccggaa taaatcggat cagataattc
      961 tgcggatccc acatagcgcg tccgatcccg tcctctcctt tgtgaacaca gctgtacagc
     1021 agctttttcg cttcaagtga atactgaaaa atcccctctc cgtcgcccca tcggtgcgat
     1081 gcaaaaaata acgccattgc aaagtattcc tctccatcag gtgccggtcc gtatgaattc
     1141 ttaactccct ccggggatac ggaccatgca aaatatcccg cattccttcc ctgatccata
     1201 tacatatatc tctttgccca cttccaaagg cagtcaaatt ccttttttct atccaactgg
     1261 acacagacca tcatcccata agacattcct tccgtacgga catcatgatt gcccgtgtct
     1321 tccatatatc ccatcgtctc atcttccgtc aggtgaaaaa atttctcttt tccatagaaa
     1381 atctccataa aagtattttc cactttccgc ctgatatcct ctttggcata tccgttttcc
     1441 aaaaatacat ttctatattc tctgctcttc tctatcatac tcccgcttcc cctttgtttt
     1501 tacgatccca tttcacagct gtacgtctac tcttttacac cgccaagcgt cagacccgtc
     1561 acaaaatacc tctgtaaaaa cggataaata catacgatcg gcaacatcgt aataatcgtc
     1621 gccgccgcac gaacagacgt tggtgtcaca cttcccgaag aatttttcat gacctctgcc
     1681 gatgtactct ggttcgtaac agaagacagc agcttcatca attcatactg cagggtcgta
     1741 aggttgtcac tcatccggtt atacagcatt gcatcaaacc aggaattcca ctgatatacg
     1801 gcgacaaata atgcgatcgt ggcatatacc ggcttgcaca gaggagaaat gatctttaca
     1861 aatatcgttg tataccccgc gccgtctaat tgtgccgact cctcaagact gtccggtatc
     1921 ccgttcatat atgtgcggat aacgagcata ttaaatgcgc tgatcattcc cggaatcaca
     1981 tatacccaaa aactgtttgt cagcccaaga tttc
//
LOCUS       DAOSYE010000428         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46379_length_2010_cov_3.534015, whole genome shotgun sequence.
ACCESSION   DAOSYE010000428 DAOSYE010000000
VERSION     DAOSYE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46379_length_2010_cov_3.534015"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1742
                     /locus_tag="PLU43_07695"
     CDS             165..1742
                     /locus_tag="PLU43_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28333.1"
                     /translation="MLKVIIADDEKLICRLVQTLADWEALGMQVAGTAENGLEALELI
                     RTLEPDILITDIRMPGCDGLELIEQAKKISPQLEVVIISGYAHFEYAQTAIRFGVGNY
                     LLKPIKQTELMDTLKSIGEKIEKTEGFLQAEQNNRQAVALLRKSLLKDMLSPHFRQLT
                     SETLKKEYYINIEAGLLQIFLIKIDETLSAKVSLSAEELLQEKVRKLLLTSMQEYCTE
                     TLLYFHMGTGYGLLQYCGKQKDAVRKKLRDCLNELNARRDLYGTVEFSIALGRTVHCA
                     KDLSLSLQDAAAAAAERLVEGVGRILEDVPEKSGIAIDAKVQEYGRQIDQVIALNDEN
                     LLKQAVAELKRKTVSTQKLCGREALELVEKAGILFISRITNENERLCFEFEKECERCG
                     SINELFSELFFMQKKLMKETAERKSSEATKPIRLAKQYVKQHFGEPVTLEEICEKTGF
                     SVSYFSALFKKETGEGFLKYLTKVRMDEAKAMLRETDLPISEICENVGYTDRKHFTHT
                     FRKATGLNPAEYRKIYG"
     gene            1735..>2010
                     /locus_tag="PLU43_07700"
     CDS             1735..>2010
                     /locus_tag="PLU43_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO28334.1"
                     /translation="MDKKYLSFRIRILAVIAAVLYLLSAAVYGYGIIRKGLSVWPLLA
                     ELILFAGLLSAAWFWVIKPYRYNEKNMQLFLDGYTTANMDELRMVELT"
BASE COUNT          598 a          355 c          555 g          502 t
ORIGIN      
        1 ggtcgtagat acgggtgtgt ctgttcttac aaaatagtaa tggattagtt tgacatcatg
       61 aaggagtgat ttcaaattcg ggaaagacgc aggatcgtaa atccggcgtt tttcttgaat
      121 tttatacagg gggatgctaa gatattttca gcagaggagt ttttatgttg aaggtgatta
      181 ttgcagatga tgaaaagttg atctgccgtc ttgtacagac actggcagac tgggaggcac
      241 tggggatgca ggtggctggg acggctgaaa acggactgga ggcgcttgag ctgatcagga
      301 cactggagcc ggatattctg attaccgata tccgtatgcc gggatgtgac ggactggaac
      361 tgatcgaaca ggcaaaaaag atcagtcctc aattggaggt cgtgatcatc agcggatatg
      421 cgcattttga gtatgcacag accgcaattc gttttggtgt cggcaattat ctgctgaagc
      481 cgatcaagca gacggaactg atggacacct tgaaaagtat tggtgaaaaa atagaaaaga
      541 cggaaggatt tctgcaggcc gaacaaaata acagacaggc ggttgccctg ctgcgtaaaa
      601 gtctgctcaa ggatatgctt tccccgcatt ttcgtcagct gacatcggaa acattaaaaa
      661 aggaatatta tattaatatt gaggcagggc ttcttcagat atttcttatt aaaatcgatg
      721 agacattatc tgccaaagtc agtctttctg cggaagagct tttgcaggaa aaggtcagaa
      781 aactgctttt aacttcaatg caggagtatt gtacggaaac cctgctctat tttcatatgg
      841 ggacaggcta tggactgctg cagtattgcg ggaaacaaaa ggatgcggtc agaaaaaaac
      901 tgcgggactg tctgaatgag ctgaatgcaa gaagggatct gtatggaaca gtggaatttt
      961 cgatcgcact cggaagaacg gtgcactgtg caaaggatct gtccctttcc ctgcaggatg
     1021 cagcggcagc ggcagcggaa cgtctcgtag aaggtgttgg aagaatttta gaagacgtcc
     1081 cggaaaaatc gggaattgca atagatgcaa aagtacagga gtatggcaga cagatagacc
     1141 aggtgatcgc gctgaatgat gagaacctgc tgaaacaggc agttgcggag ctgaagagaa
     1201 aaacagtatc cacgcaaaag ctgtgcggac gcgaggcgct ggaacttgtg gaaaaagcag
     1261 gcatattgtt tatttcacga attacgaatg agaatgaacg gctttgcttt gaattcgaaa
     1321 aagaatgtga gcgctgcggc agtattaacg aactgttttc cgaattgttt ttcatgcaga
     1381 agaaactgat gaaggagact gcggagcgca agagcagcga agctacaaag ccgatcagac
     1441 ttgccaagca atatgtaaag cagcattttg gcgaaccggt tacgctggag gaaatttgtg
     1501 aaaaaaccgg cttcagtgtc agttattttt cagcattgtt taaaaaagag actggggaag
     1561 ggtttttgaa atatctgaca aaggtcagga tggatgaagc aaaggcaatg ctgcgggaaa
     1621 ccgatctgcc catatcggaa atttgtgaga atgtcggtta tactgacaga aagcatttta
     1681 cacatacatt caggaaagcg accggattga atccggctga atacaggaag atctatggat
     1741 aaaaaatatt tgtctttcag aattcggata ttggcggtca ttgcagcagt gctctatttg
     1801 ctttctgcgg ccgtatacgg atatggaatc attcggaaag gactgtctgt ctggccgctg
     1861 cttgcggaac tgatactgtt tgcgggtctg ctgtctgcag catggttctg ggtgatcaag
     1921 ccttatcggt ataatgaaaa aaacatgcag ctatttctgg atgggtacac gacggccaat
     1981 atggacgagc ttagaatggt cgaactgacg
//
LOCUS       DAOSYE010000429         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46380_length_2010_cov_3.307417, whole genome shotgun sequence.
ACCESSION   DAOSYE010000429 DAOSYE010000000
VERSION     DAOSYE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46380_length_2010_cov_3.307417"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLU43_07705"
     CDS             <1..555
                     /locus_tag="PLU43_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOO28335.1"
                     /translation="QTVTYDGRAYLQATDQKYDVIMVDAYQDITIPFQMSTTEFFSLV
                     KEHLKKNGVMVVNMNMQTDDPDNINEYLSDTIAGVFDSVYTADVANNTNRELFASDNP
                     QMLNVFEEQLANLQEPDLKKFLAGIGPSITVYEKGEHILTDDKAPVELLGMQVIDSII
                     EDEVGYYKEKFREGDLDSLLKEVL"
     gene            670..1122
                     /locus_tag="PLU43_07710"
     CDS             670..1122
                     /locus_tag="PLU43_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOO28336.1"
                     /translation="MDSSIIAMMLAMGTAVVIFCLILYVLYVIAYWKLFTKAGEAGWK
                     SIIPIYNGYILYKIGWQVKWFWIVLGLGVISNILSAIGGNSPVVIIIVICTLMSVAAG
                     IISGIIFNLKLAKAYGKGTGFGIGLIFLNTIFVFILAFGDSKYVGAQE"
     gene            1251..>2010
                     /locus_tag="PLU43_07715"
     CDS             1251..>2010
                     /locus_tag="PLU43_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO28337.1"
                     /translation="MEQQKTDKTTEIFRDAPIPKAVFTNIIPSVVSMIMVLVYNLADT
                     FFIGQTHNAYMVAAVSLATPVFFMFMAIGMLFGIGGTSLISRMLGEGKPEKAKHASSF
                     CFWTGLAIGLLSMIFILLAGGFIANTIGASEDTFEYVRQYLFVIAFGVPFLIISNAFS
                     NIIRAEGMAGKAMMGMILGNVTNIILDPIMILGFGWNVVGAAVATVIGTIVSAVYYIY
                     HFASGKSMLSIKPADYYAGAGIATGVFAIGIPASF"
BASE COUNT          578 a          322 c          493 g          617 t
ORIGIN      
        1 cagaccgtca cctatgacgg acgtgcttat ctgcaggcga ccgaccaaaa atatgatgtg
       61 atcatggtag atgcctacca ggatattacg attccgtttc agatgtccac aacggaattt
      121 ttttctcttg taaaggagca tcttaagaaa aacggggtga tggtcgtcaa tatgaatatg
      181 cagacagacg atccggataa tattaatgag tatctgtcgg atacgattgc aggggtattt
      241 gactcagttt atacggcaga tgtggcaaat aatacgaaca gggagttgtt tgcttctgat
      301 aatccgcaaa tgctgaacgt gtttgaagaa cagcttgcaa acctgcagga gccggatctg
      361 aaaaaatttt tggcggggat cggaccgtcg atcactgttt atgaaaaggg agaacatatt
      421 ctgaccgatg acaaggcgcc ggtagaactt cttggcatgc aggtcataga cagtattatt
      481 gaagatgaag tcggatatta caaagaaaaa ttcagggaag gagatctgga ttccctgctg
      541 aaagaggttc tttgataaaa agcataattg gaatgtttaa taattgacac ataaagtgat
      601 ttggggtaaa ataataaagt gataatttat aaaaatttaa taattatttt ataaacagga
      661 gggaaaaaaa tggatagtag tataattgcc atgatgctgg caatgggaac ggcagttgtt
      721 attttttgtc tgattcttta tgtgttgtat gtgattgcat attggaaact gtttacaaaa
      781 gcaggagagg caggctggaa atcaattatt ccaatttata acggttatat attatataag
      841 attggatggc aggtcaagtg gttttggatt gttctgggtc ttggtgttat cagtaacata
      901 ttgtcggcca ttggcggaaa ttcgccggta gttattatta tcgtgatttg tacgttgatg
      961 tccgttgcag ccggaatcat cagcggtatt atatttaacc tcaagcttgc aaaggcttat
     1021 ggaaaaggta ccggattcgg tatcggactg atttttctga atactatatt tgtttttatt
     1081 cttgcatttg gcgattccaa atatgtaggg gcacaggaat agacggatca aaacaaaaca
     1141 aaagcggcag atttttctgt cgctttttct attgccatcc cggatattgt tcggtataat
     1201 aaaacagcgc gcattaaaaa agggaaataa ggaaaagaag gcagtttcat atggaacaac
     1261 aaaaaacaga caaaactact gaaattttca gagatgcacc gatacctaag gcggtgttta
     1321 caaatattat tccatctgtc gtcagtatga tcatggtgct tgtttataat ctggcagata
     1381 cgttttttat cggacagacg cataatgctt atatggtagc ggcagtttct cttgcaaccc
     1441 cggtattttt tatgtttatg gcgattggaa tgctgtttgg gatcggaggg acgtccctga
     1501 tctcaagaat gcttggggag ggaaaaccgg aaaaggcaaa acatgcttct tccttctgtt
     1561 tttggaccgg acttgccatt ggtctgctta gtatgatatt tattttgctg gccggcggtt
     1621 tcattgccaa tacgattgga gccagtgagg atacgtttga atatgtgagg cagtatttgt
     1681 ttgtcattgc attcggtgtt ccgttcctga tcatcagcaa tgcgtttagc aatattatcc
     1741 gtgcggaagg aatggctgga aaagccatga tggggatgat acttggaaac gtgacgaata
     1801 tcattctgga tccgatcatg attttgggat ttggctggaa tgttgtcgga gcagctgtgg
     1861 caacagttat cggaacgatt gtcagtgcag tttattatat ttatcatttt gcttcaggca
     1921 aatccatgct ttctattaaa ccggccgatt attatgcggg agccggtatt gcaacaggcg
     1981 tgttcgcgat cggaattccg gcttcgttta
//
LOCUS       DAOSYE010000430         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46429_length_2009_cov_2.756397, whole genome shotgun sequence.
ACCESSION   DAOSYE010000430 DAOSYE010000000
VERSION     DAOSYE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46429_length_2009_cov_2.756397"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLU43_07720"
     CDS             <1..399
                     /locus_tag="PLU43_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOO28338.1"
                     /translation="GGGSGLCSTLADYSHFALMLAGYGEYKGKRILSRKTIEYMTTDQ
                     LTQVQRNSIYFESALGYTYSNLMRIFDAKAPAASNGSYGEFGWDGLPGNYFMIDPKEE
                     LVFLYFQQIREGADLSLRRRMRNIIYGALE"
     gene            complement(473..1114)
                     /locus_tag="PLU43_07725"
     CDS             complement(473..1114)
                     /locus_tag="PLU43_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO28339.1"
                     /translation="MEQRVKKIYSKEFPDVVLRVIPGHFMTSHSHINYYVDMTYMKMR
                     QSEAQAVARAIASKYSTTAIIDTIVCMDGCEVIGAYLAEELSAAGIMSMNAHKTIYIT
                     SPEYSTGGQIIFRQNIVPMIENKNILLLFASATTGQTLLKCVECIDYYKGHVAGITSI
                     FSAIHKIADIPVESLFQISDLPDYKNYPYKDCPLCKNGVPIDAIVNGFGYSTL"
     gene            complement(1240..>2009)
                     /locus_tag="PLU43_07730"
     CDS             complement(1240..>2009)
                     /locus_tag="PLU43_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOO28340.1"
                     /translation="GEWFIRAYDAHGQKVGSRECEEGQIYIEPQGFCPLAGIGVKEGL
                     AKKALDSVKEKLDTAYGVMILQPAYTKYHLELGEVSSYPPGYKENAGIFCHNNPWISI
                     AETVIGRGDRAFEVYQKTCPAYVEEISEIHRTEPYVYSQMVAGRDAKYHGEAKNSWLT
                     GTAAWTFINISQYILGVYPTLNGLSIDPCIPKDFGDFELTRQYREGIYDIKVTNPDNV
                     EKGVSQMIIDGTAVNGCVIPYEKGKKEYHVEVIMGRS"
BASE COUNT          533 a          428 c          456 g          592 t
ORIGIN      
        1 ggaggcggtt ccggactctg ttcgactctt gcagattatt cgcattttgc cctgatgctt
       61 gcaggatatg gtgagtataa aggaaagagg atcctgagcc ggaagacgat agaatatatg
      121 acgaccgacc agctgacaca ggtccagcgg aattccattt attttgagag tgcccttggg
      181 tatacataca gtaatctgat gcgtattttt gatgcgaagg caccggcagc atctaacggc
      241 agctatgggg aatttggatg ggacggactt ccgggcaatt actttatgat agatccgaaa
      301 gaagagcttg tattccttta ctttcagcag atcagagaag gcgccgatct aagtctgcgc
      361 cgccgtatgc gcaatattat atatggtgcg ctggaataaa accgtatgat aacataaaaa
      421 gtcccccata agtgagggac ttttttgtgt atgcgcccgg cggcacacgg ttttataatg
      481 tggaatatcc gaagccgttt acaatggcat cgatcggaac tccgtttttg catagaggac
      541 agtctttata tggatagttt ttataatcag gcagatcaga tatctgaaaa agtgattcaa
      601 ccggaatatc tgcaattttg tgaatagcgc tgaagatgga tgtaataccg gcaacatgtc
      661 ctttgtaata atcaatgcac tccacacatt ttaacaaagt ctgtccggtt gtggcggagg
      721 caaacagcag aagaatattt ttattttcga tcatgggtac aatattttga cggaaaataa
      781 tctggcctcc cgtactgtat tcagggctcg tgatataaat agtcttatgg gcattcatcg
      841 acataatacc ggcggcggaa agctcttctg ccaggtaagc gccaatgact tcgcatccgt
      901 ccatgcagac aatcgtatcg ataattgcag tagtggaata tttggaagca attgcccttg
      961 cgaccgcctg tgcttcgctt tgtctcattt tcatgtatgt catatccaca taataattaa
     1021 tatgggaatg agacgtcata aagtgaccgg gaatgacgcg gagcacgaca tcaggaaatt
     1081 ccttggaata aattttttta acgcgttgtt ccatatagca cctcctattt acagttaaat
     1141 ttacatattg cattaaaaat gtcatacaat tcgataaaat aattataact gtatttacga
     1201 ctcattgcaa tagaaggtgt ctattttatt gaaactgttt taggaacggc ccatgattac
     1261 ttcaacatga tattcttttt tgcctttttc gtagggaatc acacagccgt ttacagctgt
     1321 tccgtctata atcatctgtg agacgccttt ttcaacattg tcaggattcg ttactttgat
     1381 atcgtagata ccttcacggt actgccgtgt caattcgaaa tcgccaaaat cttttggtat
     1441 gcacggatca atcgagagtc cgttcaatgt tggatatacg ccgagaatat actgtgaaat
     1501 attaatgaag gtccatgctg ccgtaccggt cagccagctg ttttttgcct caccgtggta
     1561 ttttgcatcg cgtccggcta ccatctggga ataaacataa ggttccgttc gatggatttc
     1621 gctgatttct tcgacgtacg cgggacaggt cttctgatag acttcaaatg cccggtctcc
     1681 gcgtccgatc acggtttcgg caatggagat ccatggattg ttgtggcaga agatcccggc
     1741 gttttctttg tatccgggcg gataggagct gacctcccca agttccagat gatattttgt
     1801 ataagcgggc tgcaggatca taacgccgta tgctgtatca agcttttctt tcacggaatc
     1861 aagcgctttt ttggcaagtc cttcctttac accaatgcct gcgagaggac agaagccctg
     1921 aggctcaatg tagatctggc cttcctcaca ttccctggat ccgacctttt gcccatgagc
     1981 atcataggca cggataaacc attcaccgt
//
LOCUS       DAOSYE010000431         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46431_length_2009_cov_2.685261, whole genome shotgun sequence.
ACCESSION   DAOSYE010000431 DAOSYE010000000
VERSION     DAOSYE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46431_length_2009_cov_2.685261"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2009)
                     /locus_tag="PLU43_07735"
     CDS             complement(<1..>2009)
                     /locus_tag="PLU43_07735"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOO28341.1"
                     /translation="VMGYYDGAGFTEKGSSCEIAALTQTPVILVVNAKGMSRSALAVL
                     KGFLETDTHAGIRGVIFNRLSEKLYPALKAEAEAMGITVLGFMPNVKDAVFESRHLGL
                     VGAAEIEDFKEKLEKLYQQAVGTIDWDRISAIADSAVPLSYQPESEIVPVIKPGTSVT
                     AAVAKDAAFCFTYQDNLDLLKELGCKILFFSPLTDREIPQEADAVILGGGYPELYAKQ
                     LSENRTMLASVRRAAASGIPVMAECGGFLYLLESLQDLEGRSFSMAGVLKGEGYRTDR
                     LKRFGYVYLTAEEDSLLCEKGDRLCAHEFHYYESTCNGDGLLASGTDGKRKWRAGNVG
                     PTLYAGFPHFYLYGNKKAARRFVKAAEHCHETRHPYKKTELFLSEMKKKIQETDQQAM
                     LLSRQKWNKIAKPLYGLGLFENAIVQIAGIQQTADVRIDTRAVVVMCADNGILEEGVA
                     QSSEEVTAVVAGNIAEGKACINHMAQCANAQVVAVDAGMKLTVLKDRLKVRKLMSGTR
                     NFIKEPAMSEEAMLFAFRTGVETVRDLKKEGVRIIVTGEMGIGNTTTSSAVAAVLTGT
                     DTELMTGRGAGLDDIRLKRKKEVIRQAIEKYDLKQEDTLRTLCCVGGLDLCGLAGVFI
                     GGAIYRIPIIIDGVITAAAALAAARLIPETKQYMLASHIGKEPAM"
BASE COUNT          479 a          597 c          395 g          538 t
ORIGIN      
        1 cattgccggc tcctttccaa tatgagaagc aagcatatac tgctttgtct ccggaatcag
       61 cctggctgcc gcaagtgccg cagctgccgt gatcaccccg tctatgatga tcggaatacg
      121 gtatattgca ccgccgataa acacaccggc aagaccgcac agatccaggc cgccgacaca
      181 gcacagtgtc cggagtgtgt cttcctgttt caaatcatat ttttcaattg cctgcctgat
      241 tacttctttt ttccgtttca aacggatatc atcaaggccg gctcccctgc ccgtcatgag
      301 ctctgtgtcc gttccggtaa gaactgctgc cacagcactg cttgtcgtcg tgttgccgat
      361 tcccatctca ccggtcacga ttatccgaac gccttccttt ttcagatcgc ggactgtttc
      421 cacaccggtc ctgaacgcaa acagcatggc ctcttcagac atggccggtt cctttataaa
      481 attacgcgtc ccgctcatca gcttgcggac tttaagacga tcttttaaaa cagtcagctt
      541 catgcctgcg tccacagcaa cgacctgtgc atttgcgcac tgcgccatat gattgatgca
      601 tgcctttccc tccgcaatat ttccggcaac aacagcagtc acctcctcac tgctctgtgc
      661 caccccttcc tcaaggatcc cattatccgc acacataacg acaaccgcac gggtgtcgat
      721 ccgtacatct gctgtctgct gtattccggc gatctgtacg atcgcatttt caaaaagtcc
      781 aaggccatac agcggcttgg caattttatt ccatttctgt ctgctcaaaa gcattgcctg
      841 ctgatccgtc tcctggatct tctttttcat ttccgataaa aacagctctg tttttttata
      901 aggatgtctg gtttcatgac agtgttcagc tgcttttaca aaacggcgtg cagctttttt
      961 gttcccatac aaataaaaat gaggaaatcc agcatacagc gtcggaccga cgtttcctgc
     1021 tctccacttt cttttgccgt ctgttcctga tgctaaaagg ccatccccat tgcatgtcga
     1081 ttcataataa tgaaattcat gcgcacataa acgatcccct ttttcgcaaa gcagactgtc
     1141 ttcttctgcc gtcagataca cataaccaaa ccttttcagc ctgtctgtcc gatatccttc
     1201 tcctttcagc acgcccgcca tcgaaaacga tctgccttcc agatcctgca gggattccaa
     1261 aagatacaaa aaaccaccgc attccgccat aacaggaatt ccggatgccg ccgcacgccg
     1321 gacggacgca agcatcgtac ggttttcgga taattgcttt gcataaagtt ccggataacc
     1381 gccgcctaaa ataacggcat ccgcttcctg cgggatctct ctgtcagtca gcggactgaa
     1441 aaacagaatc ttacacccaa gttccttaag cagatccaga ttatcctgat acgtaaagca
     1501 aaaagcagca tccttcgcca ctgcagccgt aacggatgtt cccggtttta tgacaggaac
     1561 gatctcagat tcaggctgat aagacagggg cactgcactg tccgcaatcg ctgaaattct
     1621 atcccagtcg atcgtcccga ctgcctgctg atacaatttt tcaagtttct ctttaaaatc
     1681 ttctatttcc gccgccccga caagtcccag atgcctgctt tcaaaaacag catcctttac
     1741 gttcggcata aaaccaagta ctgtgattcc cattgcttcg gcctctgcct tcagcgccgg
     1801 atagagtttt tcggaaagac ggttaaaaat aaccccgcgg ataccagcat gtgtatccgt
     1861 ttccagaaag cctttcagaa cggcaagcgc ggaccggctc atcccttttg catttacaac
     1921 aaggatgacc ggcgtctgcg tcaatgctgc aatttcacag ctgcttccct tttccgtaaa
     1981 gcctgctccg tcgtaatagc ccataacgc
//
LOCUS       DAOSYE010000432         2002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46706_length_2002_cov_3.322034, whole genome shotgun sequence.
ACCESSION   DAOSYE010000432 DAOSYE010000000
VERSION     DAOSYE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46706_length_2002_cov_3.322034"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLU43_07740"
     CDS             <1..678
                     /locus_tag="PLU43_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOO28342.1"
                     /translation="KDGQLACFHDNILEYRTDGTGEIADYTMDELKQLDIGYGYTADG
                     GKTYPFRGKGVGLMPSIEDVFKAFPDADLLIHIRSYSVETGEVLWSYLSQMSEERLNK
                     ITVYGSKEPLDYLRKQSPTLRVMNKEILIRAFLTYEAVGWTGYVPDAIKNTELHLPLR
                     YAKFLWGWPNKFTKRMESVNTRVVVVNGDGEWSEGFDSLADLEKLPKGYSGYVWTNRI
                     DKVADSE"
     gene            698..1255
                     /locus_tag="PLU43_07745"
     CDS             698..1255
                     /locus_tag="PLU43_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOO28343.1"
                     /translation="MFIKGLKFGMLLQFSVGPVCLFVFNIAGENGFLGGLTAMAAAIL
                     KNEKIQKIIKVFGFIILLIFGLNTIFGAFGRSLFSGPDLFSSIKTGNLFLQGILLTAS
                     NPLTILFWSGVFSTQAVAYQMNKPQLFYYGLGCVFATILFLTAVSGAGCLAHSFLSEK
                     LMMCLHIIVGSLLIVFGLKLLTKKI"
     gene            1309..>2002
                     /locus_tag="PLU43_07750"
     CDS             1309..>2002
                     /locus_tag="PLU43_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO28344.1"
                     /translation="MHNLKKQTTALTRKKKDKQAVYILMIVLQCLIYGIGNPITKIAY
                     ESITPFWCLAFRFTLAALLMAVLAGRKAIIELKAVRIGNWMPSALCMAGAYILSNLAL
                     NLTDATTVGFLMSLPVLFVPVLAVFILHRPYYRKFLPIQLAAAAGLYLLCSNGDTFYF
                     GLGEILALVTAVCTAGALVWSEKSLKSMSPVTISFVQITITAILSVTGAFLFERRTDL
                     SVIQPAAWLVILY"
BASE COUNT          523 a          383 c          514 g          582 t
ORIGIN      
        1 aaagacggtc agcttgcctg ttttcatgac aatatacttg aataccgtac ggacgggaca
       61 ggggagatcg ccgattatac gatggatgag ctgaagcagc ttgacattgg atacggctat
      121 acggcagacg gaggaaagac ctatccgttc agaggcaaag gcgttggttt gatgccctcg
      181 atcgaagatg ttttcaaggc gtttccagat gcggatcttt tgatccatat ccgttcttat
      241 tccgtggaga cgggggaagt attatggtcg tatttaagtc aaatgagcga ggagagactt
      301 aataaaatta cagtctatgg ttcaaaggag ccgcttgact atttgcggaa acagagtccg
      361 acacttcgtg taatgaataa agaaatattg atccgtgcgt ttctgaccta tgaagctgtc
      421 ggctggacag gatatgttcc tgatgcaata aaaaatacgg agctgcacct gccgctgagg
      481 tatgcaaagt ttctatgggg ctggccgaat aaatttacaa agaggatgga gtcggtaaat
      541 acaagggtcg ttgttgtaaa tggtgacggc gaatggtcgg agggcttcga cagtctggcc
      601 gatctggaaa aactgccgaa aggatacagc ggttatgtct ggacgaacag gattgacaaa
      661 gttgcagaca gcgagtagat ttttataagg agacagaatg tttatcaaag gattaaagtt
      721 tggcatgctt ttgcaatttt cagttggtcc cgtgtgcctg ttcgtgttca atatagccgg
      781 agaaaatggc tttttaggcg gacttaccgc aatggcagcg gccatactaa aaaatgaaaa
      841 aattcaaaaa atcataaaag tattcggctt tatcatatta ctgattttcg gtctgaatac
      901 aatattcggt gcatttggaa gatcactgtt ttccggtccg gatctgtttt cctccataaa
      961 aacaggaaat ctgtttttgc agggaatttt attgacagcg tcaaaccctc ttacaattct
     1021 attctggagc ggtgtctttt caactcaggc cgttgcgtat cagatgaata agccgcagct
     1081 tttttattat ggcttggggt gtgtttttgc gacgatcctg tttctgacgg cagtttccgg
     1141 tgccgggtgc ctggcacaca gctttctttc agagaaactt atgatgtgcc tgcacataat
     1201 tgtcggcagt ttattgattg tgtttggctt aaagctgctg acaaaaaaga tctgatccct
     1261 ggattacgtt aagcataaaa taaaaatttg gcagaaagag caggcatcat gcataattta
     1321 aaaaaacaga cgacagcatt aacgcggaag aaaaaggaca aacaggctgt ctatatattg
     1381 atgatcgtat tgcaatgcct gatttatggc attggcaatc ccattacaaa aatagcctat
     1441 gagagcatca cgcctttttg gtgtctggcc tttcgtttta cgctggccgc attgctgatg
     1501 gcagtccttg ccggcaggaa ggcgatcatc gaactgaaag cagtcaggat cggaaactgg
     1561 atgcccagcg cgttatgtat ggcgggtgct tatattctca gcaatctggc attgaatctg
     1621 acggacgcca cgacagttgg ttttttaatg tcgctgccgg tgctgtttgt tcctgttctt
     1681 gctgttttta tattacatcg tccttattat cggaagtttc tgcccataca gctggcagcg
     1741 gcagcaggct tatatctgct gtgcagtaat ggggatacgt tttattttgg ccttggagag
     1801 atcctggctc tggtgactgc cgtctgcacg gcaggggctc ttgtatggag tgaaaaaagt
     1861 ttgaaaagca tgtcaccggt gacgatttct tttgttcaga ttacgataac ggccatactc
     1921 tcggtcacag gggcctttct ttttgaacgc aggacggatc tgtctgttat tcagccggcg
     1981 gcatggttgg taattcttta tc
//
LOCUS       DAOSYE010000433         2001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46740_length_2001_cov_3.925488, whole genome shotgun sequence.
ACCESSION   DAOSYE010000433 DAOSYE010000000
VERSION     DAOSYE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46740_length_2001_cov_3.925488"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLU43_07755"
     CDS             complement(<1..1225)
                     /locus_tag="PLU43_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HOO28345.1"
                     /translation="MLPEQFENRMRQLLGEEFDAFLKSYDKKQYKALRLNPLKADRAQ
                     FLALYGEFLRPVLWEENGFYFTDDIQPGKHPYHEAGVYYIQEPSAMAPAGFLDVRPQE
                     KVLDLCAAPGGKSTQLAGKMAGEGLLVCNEIHPARAKILSGNIERLGVRNALVTNETP
                     QALAEAFPDYFDKILVDAPCSGEGMFRKNEEAFLQWSPENVEMCAARQDEILDCAVRM
                     LRPGGRLVYSTCTFAPLEDEGSIARFLERHPDCSVVPVKRTEGMSEGIPAFLESEAEN
                     TVRLWPHRLEGEGHFLAVLEKKSNMPAELLEWNDRGSKRGVEGDGAREFVQFQKEFLR
                     TEFNGTFLKFGTQLYLAPHGLPSLHKLKILRPGLHLGTLLKNRFEPSHALALALKPQE
                     AVHTLDLQSDGNEVRQ"
     gene            complement(1254..>2001)
                     /locus_tag="PLU43_07760"
     CDS             complement(1254..>2001)
                     /locus_tag="PLU43_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOO28346.1"
                     /translation="ASWLGFPVHYGNTVFSIFKAPDMSLLITAVVTIMPIALATMVEH
                     IGDISAISSTVGKNYIEDPGLHRTLLGDGLATMIASLFGAPANTTYGENTGVLSLTRV
                     YDPRVIRIAAYFAVLFSFSPKFEALISSMPTATIGGVSLVLYGMISAVGVRNIVENQV
                     DFSKNRNIIIAALILVLSIGIKFTTVYNAAGEEVMASGISLTFGSLTISLSGLAVGAL
                     IGIILNAVLPGKDYEFGKGAPGDTAVNFEL"
BASE COUNT          507 a          561 c          423 g          510 t
ORIGIN      
        1 actgccttac ttcatttcca tcagattgca gatcaagcgt atggaccgct tcctgcggct
       61 ttagggcaag ggcaagcgcg tgtgacggct caaacctgtt ttttaacagc gttccgagat
      121 ggagtcccgg acgcagtatc ttcagtttat gaagtgaggg cagtccatgc ggtgccagat
      181 acagctgtgt tccgaatttc aaaaacgttc cgttgaattc cgtgcgcaga aattctttct
      241 gaaactgcac aaattccctg gctccgtctc cttccactcc tctttttgac ccacgatcat
      301 tccattccaa aagctctgca ggcatgttgc ttttcttttc aagtacagcc agaaaatgcc
      361 cctctccttc aagccggtgc ggccacagac gaaccgtatt ttctgcctca gattccaaga
      421 aagccggtat tccttccgac attccttccg tcctctttac cggtacgacc gaacagtccg
      481 gatgcctttc aagaaaacgg gcaatacttc cttcatcttc aagcggtgca aacgtacagg
      541 tcgaatatac gagcctgcct cccggacgca gcatcctgac cgcgcagtct aagatctcgt
      601 cctgcctggc agcacacatt tcgacatttt caggactcca ctgcaaaaat gcttcttcat
      661 tcttcctaaa catgccttct ccggaacacg gcgcatccac aagaatctta tcaaaatagt
      721 ccggaaacgc ttctgcaagc gcctgagggg tctcattcgt tacaagggca ttcctgactc
      781 cgagccgctc gatattgccc gacaggatct ttgcccgcgc cggatggatc tcgttgcaga
      841 caagaagacc ttctcctgcc attttccctg cgagctgggt actttttcct cctggtgccg
      901 cgcaaagatc gagcaccttc tcctgcggac ggacgtcaag aaatcctgcc ggcgccatcg
      961 cactcggctc ctggatataa tacacgcctg cttcatgata cggatgcttt ccaggctgta
     1021 tatcatccgt aaaataaaat ccgttctctt cccacaaaac cggacggaga aactctccat
     1081 acagcgcaag gaactgcgcc ctgtccgctt tcagcggatt caggcgcagt gctttatatt
     1141 gttttttatc atagcttttt aaaaaggcat caaattcctc gcctaaaagc tgtctcattc
     1201 tgttttcaaa ctgctcagga agcattggat tctcctttta cagactgtct tcattacagt
     1261 tcaaagttta ccgcagtatc tcccggtgct ccctttccga attcataatc ctttccaggc
     1321 aggaccgcat tcagaataat gccgatcagc gcaccgaccg caagaccgga cagagaaatc
     1381 gtcaggcttc cgaatgtcag ggaaatacct gatgccatta cttcttcacc tgccgcatta
     1441 taaactgtcg taaacttaat gccgatcgaa aggacaagga tcagtgccgc aataataata
     1501 tttctgttct ttgaaaaatc gacctggttt tcaacaatat ttctgacacc aacagcagaa
     1561 atcattccgt acaaaacaag agaaacaccg ccgatcgttg ccgtcggcat ggacgagatc
     1621 aatgcttcaa atttcggtga aaaagagaat aaaaccgcaa agtacgccgc aattctgatc
     1681 acacgcggat catagaccct tgtcagggaa agcacgcccg tattttcacc gtacgttgta
     1741 ttagccggcg caccgaacag ggatgcgatc attgtggcaa gcccgtctcc taaaagtgtt
     1801 ctgtgaagcc ctgggtcctc gatataattt ttacctactg tggaagagat cgctgaaata
     1861 tcgccgatat gctctaccat ggttgcaagc gcgatcggca tgatcgtcac gactgccgtg
     1921 atcagcagcg acatatccgg tgccttaaat atggaaaata ccgtattccc gtagtgaacc
     1981 ggaaacccga gccaagatgc g
//
LOCUS       DAOSYE010000434         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46865_length_1998_cov_3.387545, whole genome shotgun sequence.
ACCESSION   DAOSYE010000434 DAOSYE010000000
VERSION     DAOSYE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46865_length_1998_cov_3.387545"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>528
                     /locus_tag="PLU43_07765"
     CDS             <1..>528
                     /locus_tag="PLU43_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28347.1"
                     /translation="GPVVLLLQYENFLSYMMPGDLQTAMLDALRPSISNDMFVTTGLA
                     VLLAITGFAYLFSKKKVDLYHSIPVKREKLFMASYLSGILIYLGMYAVKTAAVIMVAA
                     GRGFFTKEAGMVYLHTVLGNVIHFLLIYHVIIIAVMLTGNLLVSIAASGVLLLYGFII
                     SQVLRELSSMYFCTY"
     assembly_gap    529..538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <539..>1998
                     /locus_tag="PLU43_07770"
     CDS             <539..>1998
                     /locus_tag="PLU43_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6449 domain-containing protein"
                     /protein_id="HOO28348.1"
                     /translation="YESSNSTEAWKSAAFISPVTSFDFFINSCSEYSDVTAGQQTGHL
                     LLTLVFAAVLFIPAIYLYKKRPSEAAGKALAFQKTEVFIRIPIVIVSALVGGIVFVSI
                     NNYSKSNWFWFGLIFTGILCHCLIEIIYRFDFKAFWNHKIQLLCCLAAAILIGLIYRQ
                     DLTGYDTYLPAKEDIKSTSVVFTNIDNDLSGFQFESVDKEKFVSGKYGDRISELLRNA
                     SFSDTDAVYALGKLGIENLELMEEEDQVLYSSAREISVASAGDTAAEESTATPLYYSV
                     RYTLKNGKTRYRNYAVSVEDVSDAFARVYNNKEYKENSFDIFPMTEENAFRKVEVYDV
                     WGTKQLSLTDGEMNSFLEAYEADFLNLSGETLETEVPVARLSPIFREKDCAYDDSLGG
                     YYIYPSFIKTMTAMRELGVDLTEMKTSFDPEDLESIKVNDCGYLSDVLGITDGYSEGK
                     LYVNSDVQDKQMIEELCGDLVNSNYAWSNMVLCPFE"
BASE COUNT          558 a          346 c          480 g          604 t
ORIGIN      
        1 ccggaccggt cgtccttctt ttacaatatg aaaattttct gtcctatatg atgccgggag
       61 atctgcagac agccatgctg gacgcactgc ggccgtctat aagcaatgat atgtttgtta
      121 cgacagggct tgccgttctg cttgcaatta ccggatttgc atatttattt tctaagaaga
      181 aagtggatct gtatcacagt attccggtaa aacgggaaaa actttttatg gcatcttatc
      241 tgagtggtat tttgatctat cttggaatgt atgctgtaaa aacagcagca gtcataatgg
      301 tggcagcagg ccggggcttt ttcacaaaag aagcggggat ggtctattta catacggttc
      361 ttggaaatgt cattcatttc cttcttattt accatgtgat tattattgca gttatgctga
      421 ccggaaatct gcttgtttcg attgcggctt ccggcgtatt gctgttgtat ggatttatta
      481 tcagtcaggt attgcgggaa ctttcttcca tgtatttttg tacatactnn nnnnnnnnct
      541 atgaatcaag taatagtacg gaggcatgga aaagcgcggc attcatttca ccggtcacat
      601 cgtttgattt ttttattaac agttgttccg aatattctga tgtgacagca ggtcagcaaa
      661 ccggtcatct gcttcttacg ttagtatttg cggcagttct gtttataccg gccatttatt
      721 tgtataaaaa acgtccgtcc gaggcagcag gaaaggccct tgcttttcag aagacggaag
      781 tattcattcg aattccgatc gttatagtat cggcacttgt cggcgggatc gtatttgtca
      841 gtattaataa ctattcaaaa agtaattggt tctggttcgg tcttattttt accggcattc
      901 tttgccattg cctgatagag attatttacc ggtttgactt caaggcattt tggaatcata
      961 aaatacagct gctttgctgt ttggcagctg cgatccttat tggattgatc tacagacagg
     1021 atcttaccgg ttatgataca tacctgcctg cgaaagaaga tataaagagt acatcggttg
     1081 tgttcacaaa tattgacaat gacctgtccg gctttcagtt tgaaagtgta gataaggaga
     1141 agttcgtttc cggaaaatac ggcgaccgca taagcgagct gctgagaaat gcttcttttt
     1201 cagatacgga tgcagtctat gcgcttggaa agcttgggat agaaaatctg gaacttatgg
     1261 aagaggaaga ccaggtttta tactcttccg caagagaaat ttcggtagcg tcagcaggag
     1321 atactgcggc agaagaaagc acggcaacac cgctgtatta ttccgttcga tatacattaa
     1381 agaacggaaa gaccaggtat agaaattatg cggtgtctgt ggaagacgta agcgatgcct
     1441 ttgcaagagt ctataacaat aaagaatata aagaaaatag ttttgatatt ttcccaatga
     1501 cagaagaaaa cgcattcaga aaagttgaag tgtatgatgt gtggggaacg aaacagcttt
     1561 ctcttacaga tggtgaaatg aacagttttc tggaagcata tgaagcagat tttttgaatt
     1621 tgtcagggga aacgctggag acagaagtac cggtcgccag actgagcccg atattccggg
     1681 aaaaagactg tgcgtatgat gattcgcttg gcggatatta tatttatccg tcgtttataa
     1741 aaacaatgac tgcaatgcgg gagcttgggg ttgaccttac ggagatgaag acttcctttg
     1801 atccggaaga tctggaaagt atcaaagtga acgactgtgg atatttaagt gatgttcttg
     1861 gaataacgga tggatacagc gagggaaaac tgtatgtaaa ttcggatgta caggataaac
     1921 agatgataga ggagctgtgc ggggatcttg tcaattcgaa ttatgcatgg tccaacatgg
     1981 tattatgtcc ttttgaac
//
LOCUS       DAOSYE010000435         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46875_length_1998_cov_2.886773, whole genome shotgun sequence.
ACCESSION   DAOSYE010000435 DAOSYE010000000
VERSION     DAOSYE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46875_length_1998_cov_2.886773"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="PLU43_07775"
     CDS             <1..1790
                     /locus_tag="PLU43_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOO28349.1"
                     /translation="GRGHETYGEDPYLTSRLGVAFIKGLQGTGEFMKTAACAKHYAVH
                     SGPEAVRHEFDAQVGKKDLYETYLPAFEACVKEAGAEGVMGAYNRTNGEPCCAHSLLM
                     EKILFVDWKFKGHYVSDCWAIRDFHEHHMVTKTPEESAALAVNKGCDLNCGNTYLHIL
                     KAYREGLIKEEAITRAAVRLFTTRFLLGLFDKSEYDTITYEEVESVSHREFAVEVSEK
                     SMVLLKNNGILPLDKKKLKSIGVIGPNANSRKALIGNYHGTSSRYKTILEGIQEEADD
                     SVKIYYSEGCHLYKDRVENLAWPDDRISEAVIVAKRSDVVILCLGLDESLEGEEGDTG
                     NSYASGDKDDLKLPKSQETLLKAVSETETPVILILQTGSAMDLSFAKYHENVRAILQA
                     WYPGAEGGRAAANLLFGKKSPSGKLPVTFYETLDELPAFEDYNMAGRTYRYISGKAQY
                     PFGYGLTYSTVTLTEAKLIDYEHLKETAGHFPENLYDESFHVSVRIKNMGDCSVEEVI
                     QGYVKDLDSSFAVKNHSLCAFKRTALHAGEEKEVRLTVPARALLAVNEDGSYILDSKA
                     FTLYIGVSQPDEVSVGLCGVKPLEVSIRL"
BASE COUNT          607 a          355 c          543 g          493 t
ORIGIN      
        1 gggggagagg acatgagacc tatggggaag atccttattt gacgtccaga cttggagttg
       61 cctttatcaa ggggctgcag ggtacgggcg aatttatgaa aacagcagcc tgtgcaaagc
      121 attatgcagt acattccgga ccggaagcgg tccgccatga atttgatgcg caggttggta
      181 aaaaagacct gtatgaaaca tacctgcccg cttttgaagc ctgtgtaaaa gaagcaggcg
      241 cagaaggtgt gatgggtgct tataaccgta caaatgggga accgtgctgt gcgcatagcc
      301 tgttaatgga gaagatccta tttgtagact ggaaatttaa aggtcattat gtatcggact
      361 gctgggcgat cagggatttc catgagcacc atatggtgac aaaaacaccg gaggaatcgg
      421 cagcacttgc cgtaaacaag ggctgtgact taaattgcgg aaatacatat ctgcatatat
      481 taaaggctta cagggaaggg cttataaagg aagaagcgat tacaagggcg gcagtcaggc
      541 tgtttacgac gcggttcctg ctcggcctgt ttgataaaag cgaatatgat acgatcacat
      601 atgaagaagt ggaaagcgtt tcccatcgcg aatttgcagt tgaggtttcc gaaaaaagta
      661 tggtgctttt gaaaaataac ggaatcctgc cgcttgataa gaaaaaatta aaatcgatcg
      721 gcgtgatcgg accgaatgca aacagcagaa aagcattgat cggaaattat cacggaacgt
      781 cttccagata taagacgatt ctggaaggaa tccaggagga ggcggatgac tccgtaaaaa
      841 tatattattc ggaaggatgt catttgtata aagacagggt agaaaacctg gcctggccgg
      901 atgatcgtat ttcagaggca gtgatcgttg cgaaaagatc ggatgtagtt atcttgtgcc
      961 tcggactgga cgaatcactt gaaggtgaag aaggggacac cggaaacagt tatgcttcgg
     1021 gagacaagga tgatctgaag ctgccaaaat cacaggaaac cctgctgaag gctgtttcgg
     1081 agacggaaac accggtgatt cttattttgc agaccggcag tgcgatggat ctgtcttttg
     1141 ctaaatatca tgagaatgtc agagcgattc tgcaggcatg gtatccgggt gcggaaggag
     1201 gaagggcagc agcaaatctg ctgttcggaa aaaaatctcc gtcaggaaaa cttccggtca
     1261 cgttttatga gacgcttgac gaactgcctg cgtttgaaga ttataacatg gcaggcagga
     1321 catatcgtta tatatcggga aaggcgcaat atccgttcgg atacggactg acttacagta
     1381 cggtcacgtt gacggaagca aaactgattg attatgaaca tctgaaagaa actgccggac
     1441 actttcctga aaatttgtat gatgaaagtt ttcatgtatc cgtgcggatc aaaaatatgg
     1501 gagactgttc tgttgaggaa gtgatacagg gatatgtcaa agacctggat tcttcttttg
     1561 ccgttaagaa tcacagcctg tgtgcattta aacgcacggc actccatgcc ggggaggaga
     1621 aagaagtgcg ccttaccgtt ccggcgagag cgctgcttgc ggtgaatgag gacggcagct
     1681 atattttgga cagtaaggcg tttactcttt atatcggcgt cagccagccg gatgaagtca
     1741 gtgtcggatt gtgcggcgta aagccgctgg aagtctcaat caggctgtaa atgatgtata
     1801 attagagatc tgaataacaa aattgaaaaa gatcactgtc aaatgacagt ggtttttttg
     1861 tgttcgatag tgaaaaggat gtaactgaac gccagatata tcgcgtgtga ttatgaattt
     1921 caaggaatgt acatgattac cagaagtatt taagggcaaa aaaatgtagt aattattgaa
     1981 aaatgataca atatattt
//
LOCUS       DAOSYE010000436         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_46950_length_1996_cov_3.698609, whole genome shotgun sequence.
ACCESSION   DAOSYE010000436 DAOSYE010000000
VERSION     DAOSYE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46950_length_1996_cov_3.698609"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLU43_07780"
     CDS             complement(<1..546)
                     /locus_tag="PLU43_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOO28350.1"
                     /translation="MRNPYTVLGVRETATEDEIKRAYRSLSRKYHPDANINNPNKDAA
                     EEKFKEIQQAYQQIMDEREHGYRRTEGSYGGFGGNTYAGRERYGGGNDEYTIHMQAAG
                     NYINNGRYAEAWNILRNMEDKDALWYYYAGAANAGMGNNVQALEFAKKACELEPGNTR
                     YQMFLQQLEAGGSWYRNMQSPY"
     gene            complement(573..1406)
                     /locus_tag="PLU43_07785"
     CDS             complement(573..1406)
                     /locus_tag="PLU43_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOO28351.1"
                     /translation="MFGYVIINKQEMKFKEYEKYRSYYCGLCRSLKEQYGFGGQISLS
                     YDMTFIIMLLTALYEPDTAEGAAKCIAHPFEKHKTRRNLFTEYAADMNLLLTYEKCLD
                     DWNDERKLLRKGYADSLLHRVKKIKRKYPGKAEVIRKELAEIREIEERNEKDIDLPAE
                     AFGRIMAEILAYRPDEWEKDLRETGFYLGKFIYLMDAYEDMEKDEKTGNYNPFLLREQ
                     PVDDDAEQILLLMMADCSRAFERLPIVEDVEILRNILYSGVWCRYRLIREKKNKKVPE
                     K"
     gene            complement(1410..1805)
                     /locus_tag="PLU43_07790"
     CDS             complement(1410..1805)
                     /locus_tag="PLU43_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28352.1"
                     /translation="MKEKFRRFMTGRYGTDQFSQFLMVAALLIMLMSLFVRVNVIYWI
                     AIVLIIYAYFRVFSRNYSKRYSENQMYLKYSFRVRNFWGKIKYKVMQRKQYHIYRCPN
                     CRQKIRIPRGKGKIEIRCPKCGTEFVKRS"
BASE COUNT          482 a          540 c          321 g          653 t
ORIGIN      
        1 gtagggactc tgcatattgc ggtaccagct tccaccggct tcaagctgct gcaaaaacat
       61 ctgataccgg gtatttccag gctccagttc acatgctttt ttcgcaaact ccagtgcctg
      121 tacattattg cccatccccg catttgctgc tcctgcatag taataccaga gcgcatcctt
      181 atcttccata ttgcgcagta tgttccatgc ttccgcatag cgtccgttgt tgatataatt
      241 accggcagcc tgcatatgga tcgtgtactc gtcgttcccg ccgccatatc tttccctgcc
      301 ggcgtatgta ttcccgccaa aaccgccgta ggagccttcg gtccgcctgt acccatgttc
      361 ccgttcgtcc atgatctgct ggtatgcctg ctggatctcc ttaaattttt cttccgccgc
      421 atccttgttc ggattattga tattggcatc cggatgatat ttccggctaa gactcctgta
      481 tgccctttta atttcatctt ccgtagcagt ttcacgcact ccaagtaccg tataaggatt
      541 tctcattaat ttatatctcc gttctacccc ggctatttct ccggaacttt cttgtttttc
      601 ttttcccgta tcaaacgata ccggcaccag acaccggaat acaaaatatt ccgcaggatc
      661 tcgacatcct ctacgatcgg cagtctctca aatgctctgg aacagtccgc catcatcaaa
      721 agcaggatct gctccgcatc atcatccacc ggctgttccc gcagaaggaa cggattgtaa
      781 ttacctgttt tctcatcttt ctccatatcc tcataggcgt ccatcagata aataaacttc
      841 ccaagataaa agcctgtttc ccgcaagtcc ttttcccatt catccggacg gtacgccagt
      901 atttccgcca tgatcctgcc gaatgcttcg gccggcagat caatatcctt ttcatttctc
      961 tcttctatct cccggatctc tgcaagctcc ttacggatca cctccgcttt tcccggatat
     1021 tttcttttga tcttttttac cctgtgcaga agactgtccg catatccctt tctaagcagc
     1081 tttctttcat cgttccagtc gtccagacac ttttcataag taagcagcag attcatatct
     1141 gccgcatatt ccgtaaacag gtttcttctt gttttatgtt tttcaaacgg atgcgcaata
     1201 cattttgccg caccctcagc cgtatccggc tcatatagtg ccgtcagcag cataatgata
     1261 aacgtcatat catagcttag agaaatctgt ccgccgaacc catactgttc tttcaggctg
     1321 cggcataatc cacagtaata agagcgatat ttctcatact ctttaaactt catttcctgt
     1381 ttattgataa tcacatatcc gaacatccgt tagctccttt tcacaaactc tgtcccgcat
     1441 ttcgggcatc tgatctcgat ctttcctttc ccgcgcggaa ttcttatttt ctgacgacaa
     1501 ttcggacaac ggtagatatg atactgtttt ctctgcatta ccttatattt tatcttaccc
     1561 caaaaattcc gtacacggaa gctgtatttt aaatacattt gattttcgct gtatcttttt
     1621 gaatagtttc ttgaaaagac tctgaaatat gcataaataa tgagcactat ggcgatccag
     1681 taaatcacat ttacccttac aaataatgac atcagcatga tcagcaatgc cgcaaccatt
     1741 aaaaattgtg aaaattgatc cgttccatag cgccctgtca taaatctcct gaatttttct
     1801 ttcataccgt tcacctttct ctttctttcc ctttattcta accgttctgc aaaaaaaggc
     1861 aatgaattaa ctaaaaaatc tttctaaatt tttgctaaaa aagataccaa aaaatcgctg
     1921 aagtattacg acctcagcga tttttctttt aaaggagttt ttttacatcc ccttttatca
     1981 atcgttatca tcggat
//
LOCUS       DAOSYE010000437         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47046_length_1994_cov_2.976792, whole genome shotgun sequence.
ACCESSION   DAOSYE010000437 DAOSYE010000000
VERSION     DAOSYE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47046_length_1994_cov_2.976792"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PLU43_07795"
     CDS             <1..1618
                     /locus_tag="PLU43_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28353.1"
                     /translation="GSEISEIWAAGSRMLLCAAGSFAAAAAVGFLAARLAASFSQRIR
                     SLLFSKVDSFSMEEISRFSTASLITRSTNDITQVQMLITTGIQLLFKAPIMVIWALIK
                     IRGKGIEWTAATGAAAGIMIAVIVLLIIFVMPKFQKMQQLTDDLNRVTRENLTGLRVV
                     RAYNAERYQEEKFEDINRRLTDTQLYTNRGMAIMMPMMTTLMSGLSLVIYWIGAYLIN
                     RAEAASRLTLFSDMVVFSSYAVQIIMSFMTLAMIFILLPRASVSAKRINEVLETEPAI
                     IDGTQKDGREGMEGQVEFRDVSFKYPGASEYVLKNISFIAKKGETVALIGSTGSGKST
                     LLNLIPRFYDVGSGEVLVDGINVKEYTQESLHNKIGYVPQKAVMFRGTVKSNVELGDN
                     GRKKPESEEIAAAVRIAQGGDFVERMEKQYEADIAQGGTNLSGGQKQRLAIARAVCRK
                     PELYLFDDSFSALDYRTDRNLRTALKKETADVTSLIVAQRVGTIRDADRIIVLDEGET
                     VGIGTHKELLENCRVYREIAMSQLSEEELA"
     gene            1615..>1994
                     /locus_tag="PLU43_07800"
     CDS             1615..>1994
                     /locus_tag="PLU43_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28354.1"
                     /translation="MMGEQGIKHGPHGMAGRGSMGEAADRPRDFIAAMKRLAGYCREF
                     LPAILIALLAAAGGTFLRIIGPNKLKDMTNEIMKGLPSVINGKQMVGQIDMSVISNIA
                     WTLTLFYAAAFLLNYIQSLLMATVT"
BASE COUNT          566 a          387 c          591 g          450 t
ORIGIN      
        1 aggaagcgaa atctcagaaa tatgggcagc cggcagccgg atgctgctgt gtgcggccgg
       61 gagctttgca gctgcagctg cggtaggatt tttggctgca agactggcgg cttctttttc
      121 gcagaggatc agaagtcttc tgttttccaa agtcgattct ttttccatgg aagagatcag
      181 ccgtttttca acagcgagtc tgatcacacg ttccaccaat gatatcacac aggtgcagat
      241 gctgatcaca acagggatcc agctgttgtt taaggcgccg atcatggtaa tatgggcact
      301 gataaagatc aggggaaagg gcattgaatg gacggctgca acaggagcgg cagcagggat
      361 catgattgcg gtcatcgttc tgctgatcat atttgttatg ccgaaatttc agaaaatgca
      421 gcagctgact gatgatctga accgggtgac gagagaaaat ctgaccggtc tgcgtgtagt
      481 aagagcgtat aatgcggaaa ggtatcagga agaaaagttc gaagatataa acagaagact
      541 gacggacacc caattatata cgaacagggg aatggcgatt atgatgccaa tgatgacaac
      601 gctgatgagc ggtctgtcgc tggttattta ctggatcgga gcctatctga tcaatcgggc
      661 tgaggcggcg tcccgtctga cgctgttttc cgatatggta gtgttttcat cctatgcggt
      721 acagatcatt atgtcgttca tgacgctggc tatgatcttt attctgcttc caagagcttc
      781 cgtatcggca aaacgaatca atgaagtact ggaaacagag ccggcaatta tcgacggaac
      841 gcaaaaggac ggcagagagg gaatggaagg acaggtggaa ttcagagatg tctcattcaa
      901 atatccgggt gcgtccgagt atgtattgaa aaatatcagc tttattgcaa aaaaaggaga
      961 gacagttgcc ctgatcggtt ctaccggcag cggaaaaagc acactgctca atctgatccc
     1021 aagattttat gatgtcggca gcggagaagt tttggtggac gggatcaatg taaaagagta
     1081 tacgcaggaa agccttcaca ataagatcgg gtatgttccg cagaaggcgg tcatgtttcg
     1141 gggaacggta aaaagtaatg tggagcttgg agataacggg aggaaaaagc cggaatctga
     1201 ggagatcgct gcggcggtca ggatcgcaca gggaggtgat tttgtggaaa gaatggaaaa
     1261 acagtatgaa gcagatattg cacagggagg aacgaatctg tcaggaggac agaaacaaag
     1321 acttgccatt gcccgtgccg tttgcagaaa accggagctg tatctttttg acgacagctt
     1381 ttcagcattg gattacagaa cggatcggaa cctgcggacc gcgctgaaaa aggaaacggc
     1441 ggatgttaca agtctgatcg tcgcacagcg ggtcggaacg atcagggatg cggacaggat
     1501 cattgtttta gacgaaggag agacagttgg gatcggtacg cataaggaac tgctggagaa
     1561 ctgcagagtc tacagagaaa ttgcgatgtc acaattaagc gaggaggaat tggcatgatg
     1621 ggagaacagg gaatcaagca cggcccgcat gggatggcgg gaagagggtc catgggggag
     1681 gcagcagaca gaccgcggga ttttattgcg gcaatgaaga gactggccgg ctattgccgg
     1741 gaatttttac cggcaattct gatcgcgctg cttgcagcgg cgggaggaac ctttttgcgg
     1801 atcatcggac cgaacaaatt aaaggatatg accaatgaga tcatgaaagg acttccgtct
     1861 gtgataaacg gaaagcagat ggtcggacag atcgatatga gcgtgatctc aaacattgca
     1921 tggacgctga ccctgtttta tgcggccgct ttcttgctga actatataca gagcctgctt
     1981 atggcgactg taac
//
LOCUS       DAOSYE010000438         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47227_length_1990_cov_2.190181, whole genome shotgun sequence.
ACCESSION   DAOSYE010000438 DAOSYE010000000
VERSION     DAOSYE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47227_length_1990_cov_2.190181"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLU43_07805"
     CDS             <1..552
                     /locus_tag="PLU43_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO28355.1"
                     /translation="LRQDPDIIMIGEIRDSETAEIAVKASITGHLVISTLHTNSTAGS
                     ITRLLDMGVESYLIGDSVVGIIAQRLVRRLCPKCKRQRLATMEEKHALGFAETLELMV
                     YEPVGCKECNNIGYKGRIGVYEIMPISRKISSMVSAKMPADQIERQAITEGMTTLRRA
                     AADYVIKGITTVSEMQKVAYEEE"
     gene            593..1672
                     /locus_tag="PLU43_07810"
     CDS             593..1672
                     /locus_tag="PLU43_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOO28356.1"
                     /translation="MDYTTKAGALVMIDEVLRQAKLAGASDVHITVGISPKMRVNGRL
                     MTMQFPVLMPNDTRRLLYGIMNEKQKQAFEQKGEWDFSYSIAQLGRYRVNAFMQRGSV
                     AIVLRLVGNKIPKPQELGVPDSVVNLYNKKRGLVLVTGPTGSGKSTTLASIIGKINAE
                     LYTHIITLEDPIEYLHRHDKSIVNQREIGIDSDSYADALRSALREDPDVIFVGEMRDL
                     ETISVAITAAETGHLVFSTLHTIGAASTVDRIIDVFPPYQQQQIRTQLANVLEAVVSQ
                     QLIPRIGGAGRVAAFEVMHANSAIRNLIRESKTHQINSTIQTNRRVGMVTMDDAIVEL
                     YMRGDIDRGEAISFAQDKASMMSKV"
     gene            1701..>1990
                     /locus_tag="PLU43_07815"
     CDS             1701..>1990
                     /locus_tag="PLU43_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO28357.1"
                     /translation="MPGYQYRAIGPDGRERKGNVTAATKEQAYSKLKADGLIVVGLAE
                     ESLLTKDIDINIGGSVKARDYSVFCRQFVSIITAGVSVMNALQMLSEQTENKT"
BASE COUNT          553 a          406 c          578 g          453 t
ORIGIN      
        1 ttgcgtcagg atccggatat tatcatgatc ggtgagatcc gtgattcgga aacagctgaa
       61 attgcagtaa aagcatccat tacgggccat ttggtcattt ccacgctgca cacgaattcc
      121 accgccggaa gtattacgcg tttgttggat atgggcgttg agtcttatct gatcggcgat
      181 tccgttgtcg gtatcattgc acagcgtctt gtcagaagac tctgtccaaa gtgtaagaga
      241 cagcgtctcg ctacaatgga ggaaaaacat gcgctaggtt ttgctgaaac gcttgagctg
      301 atggtctatg aaccggttgg ctgcaaggaa tgcaataata ttggttataa ggggaggatc
      361 ggtgtttatg agatcatgcc gatcagccgt aagatcagca gcatggtgtc cgcgaagatg
      421 cctgcggatc agatagaaag acaggcaatc accgagggca tgacgacgct tcggcgtgcg
      481 gctgcagatt atgtcatcaa gggtatcaca acagtgtccg agatgcagaa ggttgcatat
      541 gaggaagagt agacggactg ttttataacg gggcgggaga tcggaggaaa ctatggatta
      601 tacgacgaaa gcaggcgcat tggtcatgat cgatgaagta ctcaggcagg caaaactggc
      661 gggcgcgtcc gatgtgcaca ttacagtcgg aatttcaccg aagatgcgtg tgaacggcag
      721 gctgatgaca atgcagtttc cggttttgat gccaaatgat acaagacggc tgttatatgg
      781 tataatgaat gaaaaacaga agcaggcgtt tgaacagaaa ggggaatggg atttttccta
      841 ttcgatcgcc cagcttggca ggtaccgcgt caatgctttt atgcagagag gttccgttgc
      901 gatcgttctg cgtcttgtcg gaaacaagat accgaagccg caggagcttg gagtgccgga
      961 ttcggtcgtt aatctgtaca acaagaaaag agggcttgtt cttgtgacag gtccgacagg
     1021 aagcggaaaa tctacaacac tggcttctat tatcggcaag atcaatgcgg agctttatac
     1081 acatattatt acgctggaag acccgatcga atatctgcac aggcatgata aatcgatcgt
     1141 aaatcagagg gagatcggaa tcgattccga cagctatgcg gatgcgctgc gttcggcatt
     1201 gcgtgaagat cctgatgtga tctttgttgg agagatgaga gatcttgaga cgatcagtgt
     1261 tgcgatcact gcggctgaaa caggtcacct tgtattttcc acactgcata cgatcggtgc
     1321 ggcgagcacg gtagacagga tcatcgatgt atttccgcct taccagcagc agcagatcag
     1381 aacacagctc gcaaatgtat tggaggcggt tgtgtcgcag cagctgatac cccgtatcgg
     1441 cggagccggc cgggtggccg cgtttgaagt catgcatgca aattcggcga tccgcaatct
     1501 gatccgtgaa agcaaaacac atcagatcaa ttcaacgata cagacaaacc gcagagtcgg
     1561 catggtcacg atggatgatg cgatcgtgga actgtatatg cggggggata tcgacagggg
     1621 cgaagcgatc agctttgcgc aggataaggc atccatgatg tcaaaggtat aaaaaatcat
     1681 gaaaacagcg ggggagtaac atgcctggat atcagtacag agccatcgga ccggatggca
     1741 gggaaagaaa aggaaatgtg acggcagcta caaaggaaca ggcctattcc aagttaaagg
     1801 cggacggcct gatcgttgtc ggacttgcgg aagagagtct tttgacgaaa gatatcgata
     1861 tcaatatcgg cgggtctgta aaggcaaggg attacagcgt attctgccgg cagtttgtca
     1921 gtattatcac tgcgggtgtc agtgtaatga acgcgctgca gatgctaagc gagcagacag
     1981 agaacaagac
//
LOCUS       DAOSYE010000439         1981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47543_length_1981_cov_2.422638, whole genome shotgun sequence.
ACCESSION   DAOSYE010000439 DAOSYE010000000
VERSION     DAOSYE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47543_length_1981_cov_2.422638"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLU43_07820"
     CDS             <1..504
                     /locus_tag="PLU43_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28358.1"
                     /translation="HRKQNGTKHILFDIKKYMLGSILLAVPIFLLLNLFFTIKPATLL
                     GVCWVLILAAMLLYATQLHAIYKKETNASDKALQKHEKKKQNGVYEEVKKKKSGFLAG
                     TAAAILALLIIFFKPVSDIYYYTGALLAGAAILYLLMGVMRDYNRLAMRKLPQFEKRG
                     GDEHADM"
     gene            491..1261
                     /locus_tag="PLU43_07825"
     CDS             491..1261
                     /locus_tag="PLU43_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOO28359.1"
                     /translation="MQICRKRLFAVIVFLSVAAFFIKTAVPVYADALYSNPDTGYEVI
                     IEDDADLLDASEEEALSQQMQKITAYGNAAFKTTDYNDTTTENYIKEYYRELFGSSSG
                     TVFLIDMYNRNIWIHSDGALYRTISSSYADTITDNVYTYASNGEYDRCADEVFSEMET
                     LLAGEKIAQPMKYISNMVLALVLALMLNYFIVRVLSAAQKPSAGELTAGTKNRYLFRD
                     PKVVLTGKSKRYSPPSSSSSGGGGSSGGGGGGGGGHSF"
     gene            1288..1746
                     /locus_tag="PLU43_07830"
     CDS             1288..1746
                     /locus_tag="PLU43_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOO28360.1"
                     /translation="MEYDHLLSMIEQQFELKGMDAHAYSPLTLAYIGDSVFDIVVKTV
                     VVETANCAANMLHRKTSAVVKAETQAKMADLLLPDLSEEEKNILRRGRNAKSGTTAKN
                     ASVSDYRKATGLEALIGYLYLMGREKRLVELIRLGMDKSETEELWHTKNI"
     gene            1725..>1981
                     /locus_tag="PLU43_07835"
     CDS             1725..>1981
                     /locus_tag="PLU43_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOO28361.1"
                     /translation="MAYEEYLIEGRNAVIEALRAKRPVDKLYILDGCQDGPILTIKRE
                     ARKTDALIKYVDKERLDQMSQTGHHQGVIAQAAAYEYAEVE"
BASE COUNT          580 a          391 c          531 g          479 t
ORIGIN      
        1 caccgcaagc agaatggaac caagcatata cttttcgata tcaaaaagta tatgcttggt
       61 tccattctgc ttgcggtgcc gatcttcctt ctgctgaatc tgttttttac gatcaaaccg
      121 gctacgctgc ttggggtctg ctgggttctg atactggcag caatgctgct ctatgcgaca
      181 cagctgcatg cgatctataa aaaggagacc aacgcatccg ataaagcgct gcagaaacat
      241 gaaaagaaaa agcagaacgg cgtgtatgag gaggtcaaaa agaaaaaatc aggctttctt
      301 gccggtacag cagcagccat tctggcactg ctcattatat ttttcaaacc ggtctctgat
      361 atttattact ataccggcgc tttgcttgca ggcgctgcga tattgtatct gcttatggga
      421 gtcatgcgtg attataacag acttgcaatg agaaaactgc ctcagtttga aaaaagggga
      481 ggtgacgagc atgcagatat gtagaaaaag attgtttgca gtgattgttt tcctgtctgt
      541 tgccgccttt tttataaaaa ccgcggttcc ggtatatgcg gatgcgctgt attccaatcc
      601 ggacacagga tatgaagtga tcattgagga tgatgctgat ctgctcgatg cgtcggagga
      661 agaagcgctt tcacagcaga tgcagaaaat aaccgcatac ggaaacgcag cgtttaaaac
      721 gacagactat aatgatacga caacggaaaa ctatataaag gaatattaca gggaactgtt
      781 cggaagcagc agcgggacag tatttttgat cgatatgtac aaccgcaata tctggataca
      841 cagtgacgga gcgctctatc gtacgatttc gtcttcttat gcggatacga tcacggacaa
      901 tgtttacaca tatgcttcaa acggagagta tgatcggtgt gctgatgaag tgttttcgga
      961 aatggagaca ttgctggcgg gtgaaaagat cgctcagccg atgaaatata tcagtaatat
     1021 ggtactggca cttgttctgg cgctgatgct caattacttt atcgtcaggg ttttatctgc
     1081 cgcgcagaaa ccaagcgcag gtgaactgac ggccggaacg aaaaaccggt atctgtttcg
     1141 cgatccgaag gttgttctta cgggcaagtc aaaacgatat tcgccgccga gctcttcatc
     1201 gtccggaggc ggaggctcat ccggaggagg cggaggaggt ggcggcggcc acagctttta
     1261 acgggattta cgatatggag aggaattatg gagtacgatc atttgcttag tatgattgag
     1321 cagcagtttg aattaaaagg tatggatgcg cacgcatact cgccgctgac acttgcctat
     1381 atcggcgaca gcgtgtttga cattgttgta aaaacagtcg ttgtggaaac agctaactgt
     1441 gcggcaaata tgctgcatag aaaaacaagt gcggtcgtga aggcagagac gcaggcaaag
     1501 atggcggatc tgctgctgcc agatctgtct gaggaagaaa aaaatatttt gcggcgcgga
     1561 cggaatgcaa aatccggcac aacagccaag aatgcttctg tttcggatta ccgaaaggcg
     1621 acaggactgg aagcgctgat tgggtatttg tatttaatgg gacgcgagaa gcgtctggta
     1681 gaattgatca gactcggaat ggataaatcc gaaacggagg aactatggca tacgaagaat
     1741 atttaataga aggaagaaat gctgttattg aggcgctgcg tgccaaaaga ccggtcgata
     1801 aactgtatat actggatggc tgtcaggacg gaccgatcct gacgatcaaa agagaagcaa
     1861 gaaaaacaga tgcattgata aaatatgtgg acaaagaacg gctcgaccag atgagccaga
     1921 ccggacacca tcagggtgtc attgctcagg cagctgctta tgaatatgca gaagtcgagg
     1981 a
//
LOCUS       DAOSYE010000440         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47796_length_1974_cov_3.175091, whole genome shotgun sequence.
ACCESSION   DAOSYE010000440 DAOSYE010000000
VERSION     DAOSYE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47796_length_1974_cov_3.175091"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLU43_07840"
     CDS             complement(<1..485)
                     /locus_tag="PLU43_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO28362.1"
                     /translation="MRIALMVFRMFFRAVYYTIKMGWIGRHDKYTEEERYAFVRKITK
                     RANKCGRVIIDAYDTKNIPEKSGFIFFPNHQGLFDVLGLIESCPTPFTIVMKKEAGNV
                     ILLKQVISCLNGLLIDRSDMKQGLQVIKKMAEDVKAGRNYVIFPEGTRTRNKNELLAF
                     KG"
     gene            complement(487..1041)
                     /locus_tag="PLU43_07845"
     CDS             complement(487..1041)
                     /locus_tag="PLU43_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOO28363.1"
                     /translation="MEKYIHTPLDENIVKNLHAGDYVYISGTIYTARDAAHKRMYEAL
                     LEGKELPVSLKDTIIYYMGPSPARDGRPVGSAGPTTASRMDKYAPALLDLGLRGMIGK
                     GKRLSSVKESMKKNNAVYFAAVGGAGALLSKCITDSKIIAYEDLGTEAIRKLTVKNFP
                     VVVVIDSEGNDLYETAVLNYRKEV"
     gene            complement(1063..>1446)
                     /locus_tag="PLU43_07850"
     CDS             complement(1063..>1446)
                     /locus_tag="PLU43_07850"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOO28364.1"
                     /translation="RLFMLKPSDGIEGVKSVILTTVKDAGPNACPPFVVGVGIGGTFE
                     KCAFLAKKALTREVNRHSEIPYVAKLEKELLEKINALGIGPGGLGGTTTAFAVNINTY
                     PTHIAGLPVAVNMCCHVNRHAVRIL"
     assembly_gap    1447..1456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1457..1914)
                     /locus_tag="PLU43_07855"
     CDS             complement(<1457..1914)
                     /locus_tag="PLU43_07855"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOO28365.1"
                     /translation="MRVINTADLTNSIKEMCVEANYFLTEDMRKCLDHAADTEEAPLG
                     KQILLQLQENLKIAGEEMIPICQDTGMAVVFLEIGQDVHFEGSSVEDAINEGVRQGYQ
                     EGFLRKSIVKDPIYRENTKDNTPSVIYYELVKGDQVKITVAPKGFGSENM"
BASE COUNT          533 a          494 c          299 g          638 t
ORIGIN      
        1 cccttgaaag ccagaagttc atttttattt ctggttcttg ttccctcagg aaaaataaca
       61 taattacggc ccgcttttac atcctccgcc atctttttta taacctgcag tccctgcttc
      121 atatcgcttc tgtcaatcaa aagtccattt aaacaggaaa tgacctgctt taataaaata
      181 acatttcccg cttccttttt cataacgatc gtaaatggag tcggacagga ttcgatcaac
      241 ccaagcacgt caaataatcc ctgatgattt ggaaaaaaaa taaaaccact tttttccggt
      301 atattctttg tatcgtaagc atctattatc acgcggccgc atttatttgc gcgttttgta
      361 atttttctga caaacgcata gcgctcttct tccgtatatt tatcatgtct tccgatccag
      421 cccattttta tagtataata aactgcacga aaaaacattc tgaataccat caatgcaatt
      481 ctcatactaa acttccttcc gataattcaa taccgcagtt tcataaagat catttccttc
      541 gctgtcaatt actacaacca ccggaaaatt ctttaccgtt aattttcgga tcgcctctgt
      601 tcccaagtcc tcataagcaa taatttttga atctgtaata catttggaaa gaagagcacc
      661 tgctccaccg actgctgcaa aatacacagc attatttttt ttcatagact ccttaaccga
      721 agaaagccgt tttccctttc cgatcatacc ccgtaaacca agatccagca atgccggcgc
      781 atatttatcc attcggctcg ctgttgtagg tcccgcagag ccaacaggtc ttccatccct
      841 tgccggagac ggacccatgt aatagataat cgtatccttt aatgaaacag gaagttcttt
      901 tccttccaga agagcttcat acattcgttt atgagccgca tcgcgggcag tatagatcgt
      961 accgctgata taaacgtaat ctcccgcatg caaatttttt acgatatttt catcaagggg
     1021 cgtgtgtata tatttttcca tctgtccttc tcttttctgt aatcataaaa ttcttacggc
     1081 atgtctgttc acatgacagc acatattgac agctaccgga agtcctgcaa tatgcgtagg
     1141 atatgtattg atattaacgg caaacgccgt agtcgttccg cccagaccgc cgggaccgat
     1201 accaagtgca tttatttttt caagcagctc cttttcaagt tttgcaacat aaggaatttc
     1261 cgaatgtctg tttacctctc ttgtcagtgc tttttttgca agaaacgcac atttttcaaa
     1321 ggtaccgcca ataccaacac caacaacaaa cggaggacag gcattaggcc cggcatcttt
     1381 tactgtcgtc aggatcacac ttttcacgcc ctcaatacca tccgaaggct tcagcataaa
     1441 caacctnnnn nnnnnnctca tattttcact tccaaaacct tttggtgcga ctgttatttt
     1501 tacctgatct ccttttacaa gttcataata aataacagaa ggcgtattat ccttcgtatt
     1561 ttctctgtag attggatcct ttacgattga tttacgcaga aatccttcct gatatccctg
     1621 tctgactcct tcgtttatgg cgtcttccac actgcttcct tcaaaatgaa catcctgccc
     1681 tatttccaaa aaaacaactg ccattcccgt atcctgacag atcggaatca tttcttcacc
     1741 ggcaattttc agattttcct gaagctgcag taaaatctgt ttaccgagcg gcgcttcttc
     1801 cgtatctgcc gcatgatcca aacatttccg catatcttcc gttaaaaaat aattcgcttc
     1861 cacacacatt tctttaatac tgtttgtcag atctgccgta ttaattactc tcatatactc
     1921 ttcctattct gtcaagccta aatatattga ttttactggc tttgacgatt tttg
//
LOCUS       DAOSYE010000441         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47808_length_1974_cov_2.570089, whole genome shotgun sequence.
ACCESSION   DAOSYE010000441 DAOSYE010000000
VERSION     DAOSYE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47808_length_1974_cov_2.570089"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..843
                     /locus_tag="PLU43_07860"
     CDS             283..843
                     /locus_tag="PLU43_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOO28366.1"
                     /translation="MNMKYALLGTLFSTPQSGHELKRSLVNSPLAPVSDTDLSIYRIL
                     LQLTREGLICSSLQTEEHRKTKTYHITDNGRQTLKDWVLSSPHAPVFMNPFMARFLSA
                     DLLNAKELEALLQKYETNLKLQILMRQESLRRFEEKNGRHSQDKLPVLMKRILDLYKN
                     ELEWINCLQAVANNDRKEPAYEQDNI"
     gene            824..>1974
                     /gene="tuf"
                     /locus_tag="PLU43_07865"
     CDS             824..>1974
                     /gene="tuf"
                     /locus_tag="PLU43_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOO28367.1"
                     /translation="MSKTIFERNKPHVNVGTIGHVDHGKTSLTAAITKVLSFSGNARY
                     TAYDSIDKAPEEKARGITISTAHVEYETDRRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLIRQVGVRHVIVFLNKIDMVDDEELLELAEMEIRELLSS
                     YEFPGDEIPIIRGSALAVLESSSANKDDAVYQPVVKLMEAVDSYIPTPERMVDKPFMM
                     HIEDVFTITGRGTVATGRIERGILHIGDEAEIIGLADDIERTVVTGIEMFRKLLDTAM
                     AGDNIGALLRGIQRSKIRRGQVLAKPGSIKPHIKFEAELYILTKEEGGRHTPFFNHYR
                     PQFFFTTTDVTGIIELPDGTDMCMPGDRMTVNVTLLTPVAIEEGTKLAVR"
BASE COUNT          617 a          419 c          439 g          499 t
ORIGIN      
        1 ctcaaaagtt atccacagat aacgaaaatg aattatgaaa tttcttcaat tatatgaata
       61 taaacaggat ttgtaaatag ataattatat tttccagttt tttattctga tttctgacaa
      121 ttatattgtt ttatttaaca aggtctccct tcataaaaat ataaatgcat gattttataa
      181 aaatctgttg actatctttt taacatatgt tattaatatc atatattaat attatcttat
      241 aatgtgcgca cgaactatta tttatcgaaa ggagaaattt ccatgaacat gaaatatgca
      301 cttttaggaa cgctttttag cacaccgcaa agcggacatg aattaaaacg aagcctcgta
      361 aactctcctc tggctcccgt atccgatacg gatctttcca tttatcggat actgcttcag
      421 cttacacggg aaggactgat atgcagcagt ctgcaaacgg aggaacacag aaaaacaaag
      481 acctaccata ttacggacaa cggcagacaa acactcaaag actgggtgct ttcctctccg
      541 catgctcctg ttttcatgaa tccgtttatg gcccgttttt tatcggcgga tctgttaaac
      601 gccaaagaac tggaagcact gctgcaaaaa tacgaaacga atctgaagct tcagatcctt
      661 atgcggcagg aatccctgcg gcgttttgaa gaaaaaaacg gcaggcattc gcaagacaaa
      721 ctgcccgttc tgatgaaaag gatcctggat ctttataaaa acgaactgga atggatcaac
      781 tgtctgcagg cagtagcaaa taatgaccgg aaggagcccg cttatgagca agacaatatt
      841 tgagagaaac aaaccgcatg ttaatgtcgg aacgatcggt catgtagatc atggcaaaac
      901 atccctgacc gcagcgatta caaaagtgct cagtttttcc gggaatgcac gctatacggc
      961 atatgacagc atcgacaaag cacctgaaga aaaagcgcgt ggaatcacga tctcaacagc
     1021 acatgtggaa tatgagacag acaggcggca ttatgcccat gtagattgcc cgggacatgc
     1081 cgattatgta aaaaacatga taacaggagc cgcgcagatg gacggtgcga tccttgtcgt
     1141 ctccgctgcg gacggaccaa tgccgcagac aagagaacac atcctgctga tcaggcaggt
     1201 aggtgtccgg catgtgatcg tatttttaaa taagatcgat atggtagatg atgaagaact
     1261 gttagaactg gcagaaatgg aaatcaggga attattatcg tcttacgaat ttccgggaga
     1321 tgagatcccg atcatcagag gctctgcttt agcagtgctg gaatcatctt cagcaaataa
     1381 agacgatgct gtctatcagc ctgttgtcaa actgatggaa gcagtcgatt cctatattcc
     1441 gacaccggaa cgcatggtcg acaaaccatt tatgatgcat attgaagatg tttttacgat
     1501 cacaggacgg ggcactgttg ctaccggacg gatcgaacgc ggaatactgc atattggtga
     1561 cgaagcggaa attatcggac tggcagacga tatcgaacga accgttgtca caggtatcga
     1621 aatgtttcga aagcttcttg atacggccat ggcaggtgac aatatcggcg ccctgctgcg
     1681 cggcatccag cgttcaaaaa tacgacgtgg gcaggtgctt gcaaaaccgg gttccataaa
     1741 gccgcatata aaatttgaag cagagcttta tattcttaca aaagaagaag gaggacggca
     1801 taccccgttt tttaatcact acaggccgca attctttttt acaacaaccg atgttaccgg
     1861 tattatcgaa cttccggacg gaaccgacat gtgtatgccc ggcgacagaa tgactgtgaa
     1921 cgtgaccctg ctcacgcctg ttgcaattga ggaaggcaca aaactcgcgg tccg
//
LOCUS       DAOSYE010000442         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_47840_length_1973_cov_3.128780, whole genome shotgun sequence.
ACCESSION   DAOSYE010000442 DAOSYE010000000
VERSION     DAOSYE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47840_length_1973_cov_3.128780"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..317
                     /locus_tag="PLU43_07870"
     CDS             3..317
                     /locus_tag="PLU43_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28368.1"
                     /translation="MIKDILTKNGYNVAGEAENGAKAVDKYNELKPDLVLMDITMPEM
                     DGIQALKKIKAGDPSALVIMCSAMGQQAMVIESIQSGAKDFIVKPFQADRVIEAVKKV
                     VG"
     gene            322..702
                     /locus_tag="PLU43_07875"
     CDS             322..702
                     /locus_tag="PLU43_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOO28369.1"
                     /translation="MVILGLLTSGLESAAQFLTVLLVFILVLILTYFTTRWVGKFQKL
                     QSNNKNFEVIETCKLTSNKYLQIVRAGKKYLVIAVGKDSVTMLTELAKEDIDLTPAGP
                     AAQDNFQKIFEKAKDKIQRRGDHL"
     gene            699..1580
                     /gene="fliP"
                     /locus_tag="PLU43_07880"
     CDS             699..1580
                     /gene="fliP"
                     /locus_tag="PLU43_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742634.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOO28370.1"
                     /translation="MNKQGMRRGKGVMIFFCLLVCAMGLFLLKPVTVKASDVNDIGIT
                     EDTENENKTVISDPGTAETADDLKELNVANNLSVTYQDGEGNLSGTLRIFIILTLIAL
                     APTLLIMMTSFTRIIIVLHFVRSALGTQTAPPNQVLIGLSLFLTFFIMNPVITQINTT
                     AVQPFEAGEINQQEFLETAAKPLREFMYGQTQTKDVRLFLDIEGIDGVESLDEIPTRV
                     LVPSFIISELRTAFIIGFLLYIPFIIIDMVVASTLMSMGMMMLPPTTISMPFKILLFI
                     LADGWNLVIGNLVKTFY"
     gene            1599..1868
                     /gene="fliQ"
                     /locus_tag="PLU43_07885"
     CDS             1599..1868
                     /gene="fliQ"
                     /locus_tag="PLU43_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOO28371.1"
                     /translation="MTVDTVTAIMRDALYTIITTGAPLLLTSLTVGLLISIFQTVTSI
                     QEQTLTFVPKIVAIFLMLMLVGHWMLNNMVDFTERLWSDFSLYIR"
BASE COUNT          549 a          338 c          481 g          605 t
ORIGIN      
        1 tgatgattaa ggacatttta accaagaacg gttataatgt tgccggagaa gcagaaaatg
       61 gtgcgaaggc agttgacaag tacaatgaat taaagccgga ccttgtattg atggatatta
      121 caatgccgga gatggatggt attcaggctc ttaaaaagat caaagccgga gatccaagtg
      181 ctcttgtcat tatgtgttcg gctatgggcc agcaggcaat ggtaattgag tccatccagt
      241 cgggtgcaaa ggattttatc gtaaaaccat ttcaggctga tcgtgtaatt gaagccgtaa
      301 aaaaagttgt aggctgatta tatggtgatt ttgggattat tgacatcggg gttggaaagc
      361 gcggctcaat ttttgacagt gcttttggtt tttatccttg tattgatact gacttatttt
      421 acaacaagat gggtcggtaa atttcagaag ctgcagtcaa acaataaaaa tttcgaagtg
      481 atcgagactt gtaaactcac atccaataaa taccttcaga tcgtgagggc aggaaaaaag
      541 tatcttgtaa tagcggttgg aaaggattcc gtcacaatgc tgacggagct tgcgaaggag
      601 gatattgatc ttactccggc aggtcctgcg gcacaggata atttccagaa gatatttgaa
      661 aaggcaaaag ataaaataca aagacggggc gatcatttat gaataaacaa gggatgcgac
      721 ggggcaaagg agtaatgatt ttcttttgcc tgcttgtgtg tgcgatgggg ttgtttctgt
      781 taaagcctgt tacggtaaaa gcatcggatg taaatgatat tggaattacg gaagatactg
      841 aaaatgaaaa taaaactgtt atcagcgatc ccggtacggc ggaaacggcg gatgacctga
      901 aagaactgaa tgtggcaaat aatttatccg ttacctatca ggacggtgaa gggaatcttt
      961 caggaacatt aagaatattt attattctga cactgatcgc gcttgcaccg acacttctga
     1021 tcatgatgac atcctttaca aggatcatta ttgtgcttca tttcgtcagg tcggcgcttg
     1081 gcacgcagac agcaccgccc aatcaggtgc tgatcggatt atcgctgttt ttgactttct
     1141 ttattatgaa tcctgttatt acacagatta atacgacggc cgtacagcct tttgaggcgg
     1201 gcgaaatcaa tcagcaggag tttttggaaa ctgcagcgaa gcctttacgt gaatttatgt
     1261 atggtcagac gcagacaaaa gacgtccggc tgtttttgga tattgagggg atcgacggtg
     1321 tggaaagcct ggatgagatt cctacaagag tacttgttcc aagctttatt atcagtgaat
     1381 tgcggactgc gtttattatt ggtttcttat tgtatattcc tttcattatt atcgatatgg
     1441 tcgtggcatc cacgctgatg tctatgggca tgatgatgct gccgcccacg acgatttcaa
     1501 tgccgtttaa gatcctgctg tttattttgg cagacggctg gaatcttgtg atcggaaatc
     1561 ttgtcaaaac gttttattag cagaagggtg tgatgaaaat gacagtggat actgttaccg
     1621 caattatgag agatgcgctc tatacgatca ttacgaccgg ggcgccgctt ttgcttactt
     1681 cattgacggt cggcctgctg atcagtattt ttcagacagt tacttccatt caggaacaga
     1741 cacttacgtt tgtacccaaa atcgttgcaa tatttctgat gctgatgctg gtaggccatt
     1801 ggatgctgaa taatatggtg gattttacgg aacggctgtg gtctgatttc tcactttata
     1861 tcaggtaaag ttttatgata gattattctt ttacatatag tgatttggaa tattttttat
     1921 tgatattatc aaggatgagc tgtttcattt ttgtggcgcc ttttttttcg aca
//
LOCUS       DAOSYE010000443         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_48019_length_1969_cov_2.592476, whole genome shotgun sequence.
ACCESSION   DAOSYE010000443 DAOSYE010000000
VERSION     DAOSYE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48019_length_1969_cov_2.592476"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLU43_07890"
     CDS             <1..262
                     /locus_tag="PLU43_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOO28372.1"
                     /translation="ELIVRHGSMFENRQVKNIRWPGKSLLVEVKHGDKNIIPKGDTEI
                     KDGDYLSILTDVNSESRITEILNGMNGAEEEEIEKNTEDTPK"
     gene            328..1434
                     /locus_tag="PLU43_07895"
     CDS             328..1434
                     /locus_tag="PLU43_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO28373.1"
                     /translation="MEKSKVYFTDMHVTGGTSLLTKLERLLKKAGFEEIDFNGNFAAV
                     KLHFGEPGNLAYLRPNYAKVVCDYVKRLGGKPFVTDCNTLYVGGRKNALDHLDSAYTN
                     GYNPFATGVHTIIADGIKGTDEYLVPVEGGEYIKEAKIGSAIMDADIIISLNHFKGHE
                     LTGFGGALKNLGMGSGSRAGKMEMHSAGKPYVRQQACIGCGKCRNICAHDAPVITNGK
                     AFIDHDKCVGCGRCIGVCPKDAVQAAGDEANDILNFKIAEYAKAVVQNRPGFHISLVI
                     DVAPYCDCHAENDIPIVPDVGMFASFDPVALDMACADAVNRQPAMPGSLLEKHGHVHH
                     DHFTDVSPDTNWMSAMEHGQKIGLGRMEYELITV"
     gene            1569..>1969
                     /locus_tag="PLU43_07900"
     CDS             1569..>1969
                     /locus_tag="PLU43_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28374.1"
                     /translation="MGYMGMERRERTTKIAAAVLLCAAALLCCAGCQESDSDMQEDGL
                     TLADNANDDAGTDADDEDNEDTVNGLSRKAYGEYLDAEEKFIGNDGVEFGIGETEQKD
                     IWIQTTIYSNIIPGAFVIDVMLPDDYDESIS"
BASE COUNT          595 a          318 c          537 g          519 t
ORIGIN      
        1 agagctgata gtcagacacg gctccatgtt tgagaaccgt caggtgaaaa atatcaggtg
       61 gccgggaaaa tccctgttag tagaagtaaa gcacggagat aaaaatataa ttccaaaggg
      121 cgatacggaa atcaaagacg gagattatct gagtattctg actgatgtaa acagtgagag
      181 cagaataacg gaaattctga atggaatgaa tggtgcagaa gaggaagaga tcgaaaaaaa
      241 tacagaagat acgcccaaat agcttgactt ggagtttact ccaggtatta caatgaatct
      301 gttaaataga attttggagg aattggaatg gaaaaatcaa aagtatattt tacagatatg
      361 catgtaacag gcggaacgag tttattgaca aaactggagc gtctcttgaa aaaagcagga
      421 tttgaagaga tcgattttaa cggtaatttt gctgcggtaa agcttcattt tggagagccg
      481 gggaatttgg catatcttcg gcctaattac gcgaaggttg tctgtgacta tgtaaagcgt
      541 cttggcggaa aaccatttgt aacagattgt aatacgcttt atgtcggcgg acgtaaaaat
      601 gcacttgatc atctggacag tgcttatacg aacggctata atccgtttgc aacaggtgtt
      661 catacgatca ttgcggatgg aatcaaaggc acggatgaat atcttgttcc ggtagaaggc
      721 ggagagtata taaaggaagc taagatcggt tccgcaatta tggatgcgga tattattatt
      781 tccctgaatc attttaaggg acatgaattg accggctttg gcggtgcgct gaagaatctt
      841 ggcatgggca gcggttcaag ggccggcaaa atggaaatgc acagtgccgg gaaaccgtat
      901 gtgagacaac aagcttgtat tggctgcggg aaatgccgga atatctgtgc acatgatgcc
      961 ccggttatta caaatggaaa agcatttatc gaccatgata aatgtgtcgg ctgcggccgc
     1021 tgtatcggtg tatgtccaaa agatgcggta caggctgcgg gtgatgaggc aaacgatatt
     1081 ttaaacttta aaattgcaga atatgcaaag gccgttgtgc agaaccgtcc gggctttcat
     1141 atcagtcttg ttattgatgt ggcaccgtac tgtgactgtc atgctgagaa tgatattccg
     1201 atcgtaccgg atgtggggat gtttgcgtca tttgacccgg ttgcactgga tatggcatgt
     1261 gcagatgcgg tcaaccgtca gcctgctatg ccgggaagtc ttcttgagaa acacggacat
     1321 gtgcatcatg atcatttcac agacgtatct cctgatacga actggatgtc ggcgatggag
     1381 catggtcaga agatcggtct tggcagaatg gagtatgaac tgatcaccgt gtaacgaaaa
     1441 tgttaagtga caattgtaat ataaggatat gatgttcgta tcttgtccgt aaaatataat
     1501 tgcggtaaaa tttagacagt tgttccgtta tttcggaata actgtctgtt tatttttatg
     1561 agagaaggat gggttatatg gggatggaga gaagagagag gactacgaaa attgctgcgg
     1621 cagttttgct gtgtgcggca gctttattgt gctgcgccgg ctgtcaggaa tcagattccg
     1681 atatgcagga ggatgggctg acattggcgg acaatgcaaa tgatgatgcg ggtacggatg
     1741 cggatgatga agataatgag gataccgtaa acggactgtc aagaaaagcc tatggcgaat
     1801 atcttgatgc agaggaaaaa tttatcggaa atgacggagt agaatttgga attggggaga
     1861 cggagcaaaa agacatctgg atacagacaa cgatctattc caatattatt ccgggcgcat
     1921 ttgtaatcga tgtgatgctt ccggatgatt atgatgaaag tatttccta
//
LOCUS       DAOSYE010000444         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_48071_length_1968_cov_2.135389, whole genome shotgun sequence.
ACCESSION   DAOSYE010000444 DAOSYE010000000
VERSION     DAOSYE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48071_length_1968_cov_2.135389"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLU43_07905"
     CDS             <1..226
                     /locus_tag="PLU43_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO28375.1"
                     /translation="GMGFGMMPMFAGVMELAGRGIVAVIAAQFLSFKGIAAANPAAWI
                     LADLLLIPSYIVLFKKLPKGRSESTAESAV"
     gene            314..649
                     /locus_tag="PLU43_07910"
     CDS             314..649
                     /locus_tag="PLU43_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO28376.1"
                     /translation="MTEKIVERNLLYDFYGELLTEHQRKIYEDAVYGDLSLSELSEEY
                     GITRQGVHDLLKRCDKILSGYEGKLHLLEKFRETKELTAQIHKLAVSRDAAAFDSIAA
                     ISEKIFEIL"
     gene            666..>1968
                     /gene="ffh"
                     /locus_tag="PLU43_07915"
     CDS             666..>1968
                     /gene="ffh"
                     /locus_tag="PLU43_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOO28377.1"
                     /translation="MAFENLTEKLQNVFKSLKSKGRLTEADVKTALKDVKMALLEADV
                     NFRVVKQFIKAVEERAVGQDVMNGLNPGQTVIKIVKEEMVSLMGSETTEISLQPGKAV
                     TVIMMCGLQGAGKTTTAAKIAGKLKQKGKKCLLVACDVYRPAAIKQLQINGEKQDVEV
                     FSMGDSHKPVDIVKAALEHANKNGHQVVILDTAGRLQIDEGMMAELAEIKAAVTVHQT
                     LLVVDAMTGQEAVNVAKSFDEQIGVDGVVLSKMDGDARGGAALSVKAVTGRPILYVGM
                     GEKLSDLEQFFPDRMAGRILGMGDVLTLIEKLQTDVDEEKEKQMARKLKKGKFDFEDY
                     LESMSQMKNMGGISGIMNMMPQLGNNMKSGDIESLVDEKKLAHMEAIVLSMTKRERRE
                     PDILNPSRKHRIAKGAGVDISEVNRFIKQFEQTRKLMKQIPG"
BASE COUNT          601 a          352 c          556 g          459 t
ORIGIN      
        1 gggaatgggc tttggcatga tgcccatgtt tgccggtgta atggaactgg cggggagagg
       61 gatcgtggcg gtgatcgccg cacaattttt aagctttaag ggaatcgcag ccgccaatcc
      121 ggccgcatgg atcctggcag accttctttt gataccgtcc tatatcgttc tttttaaaaa
      181 gcttccgaag ggaaggtccg aatcaacagc agaatcagcg gtgtaaagaa ataatacttg
      241 acaccggcag ccctgttgtg tatgatagga aatgtgtaaa aattcatgga gatttattta
      301 cgggtttttc attatgacgg aaaaaatagt tgaacgaaat ttattatatg atttttacgg
      361 agaactgctg acagagcatc agcgtaagat ctatgaggat gcggtatacg gagatctctc
      421 attgtcggaa ttatccgaag agtacgggat cacaagacag ggtgtccatg atctgctgaa
      481 gcgctgcgat aagattctga gcggttatga gggaaagctg catttgcttg aaaaattccg
      541 ggagacaaag gaactgacag cgcagatcca taagctggcc gtcagccggg atgctgcagc
      601 atttgacagt attgctgcca tatcggaaaa aatatttgag attttataat aatggagttt
      661 tatacatggc ttttgaaaat ttaacggaaa aactgcagaa tgtatttaaa agtttaaaga
      721 gtaaaggacg tttgacggaa gcagacgtga aaacggcttt aaaagacgtg aaaatggcac
      781 ttcttgaggc tgacgtcaat ttcagggtcg taaaacaatt cataaaagcg gtcgaagagc
      841 gtgccgttgg acaggatgtc atgaacggac tgaatcctgg acagacagtt ataaagatcg
      901 taaaagaaga aatggtttcc ctgatgggca gtgaaacaac agaaatcagc ttacagccgg
      961 gaaaggccgt taccgttatt atgatgtgcg gtctgcaggg agccggaaaa acaacaacag
     1021 cggcgaaaat agccgggaag ctgaaacaga agggaaagaa atgtctgctt gtggcatgtg
     1081 acgtatatcg tccggccgcc attaagcagc tgcagatcaa cggtgaaaaa caggacgtcg
     1141 aagtgttctc gatgggagac agccataagc cggttgatat tgtgaaggct gccctggagc
     1201 atgcaaataa aaacggacat caggtcgtga ttctggatac tgcaggcagg ctgcagatcg
     1261 atgaaggcat gatggcagag cttgccgaga tcaaggcagc ggtcacggtc catcagacac
     1321 tgcttgtcgt tgacgcgatg accggccagg aagctgtcaa cgttgcgaaa agctttgatg
     1381 aacagatcgg tgtagacggt gttgttttat cgaaaatgga cggcgatgca agaggcggtg
     1441 cagcactttc tgtcaaggcg gtaaccggca gaccgatctt atatgttggt atgggtgaga
     1501 agctttcgga tctggaacaa ttttttccgg accgcatggc cggccgtatt cttggtatgg
     1561 gagatgtcct gacgcttatt gaaaagctgc agacggatgt tgatgaagaa aaagaaaagc
     1621 agatggccag aaagctgaaa aaagggaaat ttgactttga agattatctg gaaagcatgt
     1681 cgcagatgaa aaatatgggc ggcatatcag ggatcatgaa tatgatgccg cagcttggaa
     1741 ataacatgaa atccggagat attgagtctt tggtagatga gaaaaagctg gcgcacatgg
     1801 aagcgatcgt tctttccatg acaaagagag agcgccggga gccggatatt ttaaatccga
     1861 gcagaaaaca cagaattgca aaaggggccg gtgtggatat ttcggaagtc aaccgtttta
     1921 tcaagcagtt tgagcagacg agaaaactga tgaagcagat cccgggta
//
LOCUS       DAOSYE010000445         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_48098_length_1967_cov_3.000523, whole genome shotgun sequence.
ACCESSION   DAOSYE010000445 DAOSYE010000000
VERSION     DAOSYE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48098_length_1967_cov_3.000523"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1967
                     /gene="lon"
                     /locus_tag="PLU43_07920"
     CDS             <1..>1967
                     /gene="lon"
                     /locus_tag="PLU43_07920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOO28378.1"
                     /translation="YTVGTIAVIKQVLKLPNNIVRVLVEGISRAELSAVDEEESNYLL
                     ASVKPYPQPASELIAVEEQAELTAMVRTLQELLAEYFVYNPRMSKTLEKQFEESKNLS
                     ELLDFIAVNFPMAQTDRQHLLEAVDVKERFSVLTTILTREVEVLGIKKNLQEQVKERV
                     EKNQKEYVLREQMKYIKEELGESGPDSDTDRFRESLKKLDAPKEVREKIGKEIDRFSQ
                     INSSSSESAVSRGYIETLLEMPWNKVSKDNKDVDNAERILNEDHYGLKKVKERMLEFL
                     SVRNLTKKGDSPIICLVGPPGTGKTSVAKSVARALDKEYIRISLGGVRDEAEIRGHRR
                     TYVGAMPGRIAMGLKNAGVKNPLILLDEIDKTSSDYKGDTASALLEVLDSEQNSKFRD
                     HYIELPIDLSEVLFIATANSAADIPRPLLDRMEVIEVTSYTENEKVHIAREHLIKKQL
                     ERHGLKPEQMVIQGKALERIVRNYTREAGVRNLERRIGEICRKTARQICQEHKTGVKV
                     TLRNLEKFLGKERYQYDMANETDEIGIVRGLAWTSVGGDTLQIEVNVMPGKGEILLTG
                     QLGDVMKESAQAGISYIRSISRDHKIAADFFKKNDFHIHIPKGSVPKDGPSAGITMAT
                     ALYSAITNRPVHADVAMTGEITLRGEVLPIG"
BASE COUNT          586 a          390 c          558 g          433 t
ORIGIN      
        1 tttatacggt cggcacgatc gctgtgatca agcaggtgct gaagctgccg aacaatatcg
       61 tacgggtgct cgtggaagga atttcgcgtg ctgaactgag tgcggtcgat gaagaggaaa
      121 gcaattatct gcttgcttct gtaaaacctt atccgcagcc ggcttctgag ttaattgccg
      181 tggaggagca ggcagagttg actgcaatgg tcagaacttt gcaggagctt ttggcagagt
      241 actttgtgta taatccgcgc atgagcaaaa cattggagaa gcagtttgaa gagtctaaaa
      301 atttatctga attgcttgat tttattgcgg tcaattttcc gatggcacag actgacaggc
      361 agcacctgct ggaagctgtg gatgtgaagg aacgattttc tgttttgacg acaatattga
      421 ccagggaagt tgaggtcctt ggcatcaaaa agaatttgca ggaacaggta aaggaacggg
      481 tcgaaaaaaa tcaaaaggaa tatgtgctgc gtgagcagat gaaatatata aaagaggagc
      541 tcggggaaag cggaccggat tccgataccg accgcttcag ggaatccttg aaaaagctgg
      601 atgcaccaaa ggaagtaagg gaaaagatcg ggaaagagat agaccgattc agtcaaatca
      661 attccagctc ttctgaaagc gcagtatcca gaggctatat tgagactttg cttgaaatgc
      721 cgtggaataa agtatcaaaa gacaataaag atgtggataa tgccgaacgg atcttaaatg
      781 aagatcatta tggcctgaaa aaagtcaaag aacggatgct ggaattttta tccgtacgga
      841 atctgacgaa aaaaggagac agtccgatca tctgtctcgt cggcccgccc ggaactggta
      901 aaacttcggt cgcaaagtcg gttgcacgcg cacttgataa ggaatatatc aggatcagtc
      961 ttggcggcgt tcgggacgaa gcggagatcc gcggtcatcg caggacctat gtaggggcaa
     1021 tgcccggcag gatagcgatg ggactcaaaa atgccggagt gaaaaatccg ctgatcctgc
     1081 ttgatgagat cgacaagacg agcagtgatt ataaagggga taccgcatcg gcactccttg
     1141 aagtgcttga cagtgaacaa aattccaaat tccgcgatca ttatatcgaa ctgccgatcg
     1201 atctttccga agtgctgttt attgcgacgg cgaattctgc ggcagatatt ccgcggccgc
     1261 tgcttgaccg tatggaagtg atcgaggtta caagctatac tgagaatgaa aaagtacata
     1321 ttgccagaga acatctgatc aaaaaacagc tggagagaca tgggttgaag ccggaacaga
     1381 tggtcattca gggtaaggca cttgaaagga tcgtgcgaaa ctatacgcgt gaagcgggtg
     1441 tccggaatct ggagcgcagg atcggtgaga tctgcagaaa aaccgcccgt cagatctgcc
     1501 aggagcataa aacgggtgta aaggtgacgc tgaggaatct ggagaaattt cttggaaaag
     1561 aacgctatca atacgatatg gcaaacgaga ccgatgagat cgggatcgtg cgcggactgg
     1621 catggacaag cgtcggcgga gatacactgc agatcgaagt caacgtcatg cccggaaaag
     1681 gagaaattct tctgacaggc cagcttggag atgtgatgaa ggagtcggcg caggccggta
     1741 tcagttatat ccgctctatc agcagggacc ataaaattgc cgctgatttt ttcaagaaga
     1801 atgattttca tatccatatt ccaaagggct ccgtgccaaa ggacggccca agtgccggca
     1861 ttacaatggc aacggcgctg tactcggcaa ttacgaacag gccggtgcat gcggatgtgg
     1921 caatgactgg agagatcacg ctacgtggtg aggtgctacc gataggt
//
LOCUS       DAOSYE010000446         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_48706_length_1951_cov_2.896624, whole genome shotgun sequence.
ACCESSION   DAOSYE010000446 DAOSYE010000000
VERSION     DAOSYE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48706_length_1951_cov_2.896624"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1090)
                     /locus_tag="PLU43_07925"
     CDS             complement(26..1090)
                     /locus_tag="PLU43_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28379.1"
                     /translation="MTKYPVIGIRPIIDAREMGIREQLEGKARTMAENAKALIEEQIK
                     YGDGTPCRCVIYSGTIGCGEEAARCAAEFSGQNVCATLSVTPSWCYGAETMDIDTLTI
                     KAVWGFNGTERPGAVYLACVMAAHNQKGLPAFSIYGHDVQDMEDDSVPDDVREKLLLF
                     AKCAVTAGALREKAYVGIGAVSMGIMGSYCDPSFYQKYLGMRTEWVDMTEVLRRIKKG
                     IYDEEEYQKALAWVKENCREGAEPGPNVRDNRMHTKEQKEQDWEFVVRMTLIIRDILI
                     GNEKLAAMGYKEESFGRNAAVGGFQGQRMWTDWLPNCDFTEAMLNSTFDWNGKKQPLA
                     FATENDNLNGICLLFSNLRL"
     gene            complement(1116..>1951)
                     /locus_tag="PLU43_07930"
     CDS             complement(1116..>1951)
                     /locus_tag="PLU43_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO28380.1"
                     /translation="KVKELGFDILEVSCAAIAKMGQEELSALKEEAKAYDVMLTAGYG
                     PAANENLGSAEEAVVKNAVAFYTSVLKKLEFLDIHTIGGGIYSYWPVDYTKPVDKQGD
                     WERSVKNVRTVGRIAEACGVEYCLEVLNRFEGYLLNTCAEAKTFVEQVGEPNVKIMLD
                     TFHMNIEEDDMIEAILLAGDKLGHFHVGENNRRVPGKGGLPWYRIGSALRAIGYQKNV
                     VMEPFVKSGGTIGSDIKVWRDLSAGASMEQLDKDARDSAAYLRYVFSGPNSNEQSILK
                     V"
BASE COUNT          450 a          594 c          350 g          557 t
ORIGIN      
        1 ggaaaaaaaa caacattatt acaaattaca accgcagatt ggaaaacaac agacagatcc
       61 cgttcagatt atcattttcc gttgcaaaag caagcggctg cttctttccg ttccagtcga
      121 acgtcgaatt gagcatagct tctgtaaaat cacagttcgg aagccagtcc gtccacatcc
      181 gctgtccctg aaaaccgcca acggccgcat tcctgccaaa agactcttct ttatagccca
      241 ttgcggcaag cttctcattg ccgatcagaa tatccctgat gatcagtgtc atacgcacga
      301 caaattccca gtcctgctcc ttctgttcct ttgtatgcat cctgttgtcc cgcacattcg
      361 gaccaggttc tgctccttcc ctgcagtttt cttttaccca ggccagtgct ttctgatatt
      421 cttcctcatc ataaattcct ttttttatcc ggcgaaggac ttccgtcata tcgacccatt
      481 ccgtccgcat gcccagatat ttctgataaa acgacggatc acagtaagag cccataattc
      541 ccatggacac agcaccgatc ccgacatatg ccttttcacg cagagcacct gccgtaaccg
      601 cacattttgc aaacagcagc agcttttccc tgacgtcgtc cggcacggaa tcgtcttcca
      661 tgtcctgtac gtcatgtccg taaatcgaaa atgcaggcag gcctttctgg ttatgtgccg
      721 ccatgacaca tgccagatat acggctcccg gtctttcggt tccattaaat ccccagactg
      781 cttttattgt cagcgtatcg atatccatcg tttccgctcc ataacaccag ctcggtgtaa
      841 cactcagcgt cgcgcacaca ttctgtccgg aaaactccgc cgcacacctt gcggcctctt
      901 ccccgcaccc gatcgtgccg gaataaataa cacatctgca cggcgttccg tctccgtatt
      961 tgatctgttc ctcgatcagt gccttcgcat tttcagccat tgttctcgct tttccttcaa
     1021 gctgctctct gattcccatt tctctggcat cgatgatcgg cctgataccg atcacaggat
     1081 actttgtcat tctgtttccc tccttgcgat tgcactcata ctttcagtat tgactgctca
     1141 ttggaatttg gtccgctgaa aacatatctt aaataggcag cggaatccct ggcatctttg
     1201 tcaagctgct ccatggatgc accggcgctt aagtctctcc atactttaat atcggacccg
     1261 atcgttccgc cgctctttac gaacggctcc atcacaacat ttttctgata tccgatcgcc
     1321 ctgagagcac tgccgatccg ataccacggc agaccgccct tgcccggcac acgtctgtta
     1381 ttttcaccga catggaaatg tccgagctta tcaccggcaa gcaggatcgc ttcgatcatg
     1441 tcatcttcct cgatgttcat atgaaacgta tccagcatga tctttacatt cggttcccct
     1501 acctgctcga caaatgtttt cgcttccgcg catgtattca ataaatatcc ttcaaaacgg
     1561 ttcagcactt ccagacaata ttccacaccg catgcttccg ctattcttcc aacagtacgg
     1621 acatttttca cacttcgctc ccagtccccc tgtttatcga ccggctttgt ataatcaaca
     1681 ggccaatacg aataaatacc gcctccgatc gtatgaatat caagaaattc cagctttttt
     1741 aatacggacg tataaaaggc gaccgcattt tttacgactg cttcctctgc cgaaccaaga
     1801 ttttcatttg ccgccgggcc gtaaccggcc gtcagcatca catcatatgc ttttgcttct
     1861 tccttcagtg cactcagctc ttcctgcccc attttggcaa ttgccgcaca ggacacttcc
     1921 agaatatcaa atccgagttc ttttaccttt t
//
LOCUS       DAOSYE010000447         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_49102_length_1942_cov_2.455220, whole genome shotgun sequence.
ACCESSION   DAOSYE010000447 DAOSYE010000000
VERSION     DAOSYE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49102_length_1942_cov_2.455220"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLU43_07935"
     CDS             <1..519
                     /locus_tag="PLU43_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28381.1"
                     /translation="LICAVVCWFFYQLAQYRGIPTVLAWVFLIVLVYGYITSNTTVGR
                     YFYAVGGNEKATELSGINTKMVYFRAYVNMGFLSAIAGMITIARMTSAQPTYGQNYEM
                     DAIGSCFIGGASAYGGVGTVPGVIVGAVLMGIINLGMSIMSVDANYQKVVKGLVLLAA
                     VIFDVLTKKESK"
     gene            653..>1942
                     /gene="uvrC"
                     /locus_tag="PLU43_07940"
     CDS             653..>1942
                     /gene="uvrC"
                     /locus_tag="PLU43_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOO28382.1"
                     /translation="MFDIEEELKKLPKKPGVYLMHDENDSIIYVGKAIVLRNRVRSYF
                     RESTNKTMKIRQMVRKISWFEYVVTDSEMEALVLENNLIKEHQPRYNTMLKDDKTYPY
                     IKVTTQETFPRIFLTREVKKDNAKYYGPYTSVNAVKDTIELLRKLYRLRTCSKKLGNT
                     PCLYYHIGQCDAPCLGRISEETYGESVQKALEFLNGNYKPLLSELEEKMQEASERMDF
                     EKAGEYRDLLFSVRQTAQKQKITDAAGEDKDVIAMARDEKSVIIQVFFVRSGKILGRE
                     HFYMKNTEDTEDAGTIKSFIMQYYAGTPFIPKELILPFEPEDRELIEQWLTDKRGAKT
                     HITIPQKGKKEKLAELAKKNAQIVLRQDIDKIRREDARTGGAVKEIERLLGLSGIVRM
                     EAYDISNISGFESVGSMIVYEKGKPRRNDYRKFRIRSV"
BASE COUNT          576 a          353 c          502 g          511 t
ORIGIN      
        1 ctgatctgtg cggtcgtatg ctggttcttc tatcagctgg cgcagtacag aggaattccg
       61 acagtgcttg catgggtatt cctgatcgta ctcgtttatg gttatattac atccaatacg
      121 acggtcggcc gttattttta cgcagtcggc ggaaatgaaa aagcaacgga gctttccggt
      181 atcaatacaa aaatggttta tttccgggca tatgttaata tgggcttctt atctgccata
      241 gccggcatga ttacgatcgc gcgtatgaca tccgcacagc cgacatatgg tcagaactat
      301 gagatggacg caatcggttc ctgttttatc ggcggtgctt ccgcatacgg cggagtcggt
      361 acggttccgg gtgtcattgt aggtgctgtt ctgatgggta ttatcaacct cggtatgagt
      421 attatgagtg tggatgccaa ctatcagaaa gttgtcaaag gtcttgtact tcttgcagca
      481 gttatctttg atgttttaac aaagaaagaa tccaaataaa gagatatatt gttttttttc
      541 atatatctaa tcagaggagg ggcttgttgt gcatgcaata agcccttttc cttttccgta
      601 gtttcttttt ctccttgatt ctgttatatt attggaggaa aggatgaatg ctgtgtttga
      661 tatagaagaa gaattgaaaa agcttccgaa aaagccgggc gtatatttga tgcatgatga
      721 gaacgattcg atcatttatg tgggaaaagc aatcgttctt aggaatcgtg tgcgttctta
      781 ttttcgggaa agtacaaata aaacaatgaa gatccggcag atggtgagaa aaatcagctg
      841 gtttgaatat gtagtgacgg attccgagat ggaagccctt gtgctggaaa ataatttgat
      901 caaggagcat cagccaaggt ataatacgat gctcaaagac gacaagacat atccttatat
      961 taaggtgacg acgcaggaga cgtttccgcg gatctttctg acaagggaag taaagaaaga
     1021 caatgcgaag tattatggcc cttatacgtc cgtcaatgcc gtaaaggata cgatcgagct
     1081 tttgagaaag ctgtaccgtc tccgtacgtg ttcaaaaaag cttggaaaca cgccgtgcct
     1141 ttattatcat atcggtcagt gcgatgcgcc ctgcctcggc agaatcagtg aagagacata
     1201 tggagaatct gtacagaagg ctttggaatt cttaaatgga aattacaaac cgctgctttc
     1261 cgagctggaa gaaaaaatgc aggaagcgtc cgaaagaatg gattttgaaa aggccggtga
     1321 atatcgggat ctgcttttca gtgtgcgtca gaccgcgcaa aaacaaaaga tcacggatgc
     1381 tgccggtgag gataaagatg tgattgccat ggcaagagat gagaaatcgg tcattattca
     1441 ggtgtttttt gtccgcagcg gaaagattct gggacgagag catttttata tgaaaaatac
     1501 ggaagacaca gaggatgccg gtactataaa aagctttatc atgcagtatt atgcgggcac
     1561 gccgtttatt ccaaaagagc tgatccttcc gtttgaaccg gaggaccggg agctgatcga
     1621 gcaatggctg acagacaagc gcggagcaaa gacgcatatt accataccgc agaaaggaaa
     1681 aaaagaaaaa cttgcggagc ttgcgaaaaa aaatgcgcag atcgtactta gacaggatat
     1741 cgacaagatc aggcgggagg atgccagaac aggcggtgcc gtgaaagaaa tagaaaggct
     1801 gcttggcctt tccggtattg tgcggatgga agcctatgat atttcaaata tcagcggctt
     1861 tgagtcggta gggtccatga tcgtatatga aaaaggaaaa ccaagacgga atgactacag
     1921 gaagttcaga atcagatccg ta
//
LOCUS       DAOSYE010000448         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_49323_length_1937_cov_2.470244, whole genome shotgun sequence.
ACCESSION   DAOSYE010000448 DAOSYE010000000
VERSION     DAOSYE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49323_length_1937_cov_2.470244"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLU43_07945"
     CDS             complement(<1..742)
                     /locus_tag="PLU43_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO28383.1"
                     /translation="MSEKMKVVVIADERKVEVREVRKPSPKPGQILMKINACAICTWE
                     QRTFTRESHMPLPFVGGHEIAGEVAALGAGVRETEFPVGSKIVARLINVCGKCYFCRR
                     GEENLCVELNNLDNSEMEIPGTGGLGEYLTINVSQAYQIPEELSYKTAVFAEPLACVL
                     NSIEKGRIELGDDVVVIGGGIMGMLHILGAKLSGARVILSEPDADRRRIAKELGCDIT
                     LNPLEKDPVEFIKNLTQGRGAEAVFNTTP"
     gene            complement(779..1918)
                     /locus_tag="PLU43_07950"
     CDS             complement(779..1918)
                     /locus_tag="PLU43_07950"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO28384.1"
                     /translation="MQYAAYRTTQYVLGNGSIETLSEYKDKKLAFVIDGGILEALHLK
                     QILFENLLKDSDYRILCDMRTEPHMEMLQGPIEEVRAFGPDIIVGIGGGSVMDTAKAL
                     WLLYELPEYDWEKAFKAYEVAPFPGKAKLIVVPTTSGTGSETTGCAVMKDAQKKKRMI
                     LSNEIIPTTAIMDFDLLKSLPKGNIAFSGTDALAHALEAGASRLASTMVRMQCIQAAV
                     TLIKELPKSMEGDMEAREKVHIAATMAGGGINNSITGMAHGMDQAGGDFGKPHGMMTG
                     MLLPYTMEYLIPQPIYEEVADQLGITGQSEEKQRILTERIWELYRKLHMPITLQEAGI
                     PEADYLKRIPLYIEAALQDANIMFAPKDPDQEELEALYKKFYYGK"
BASE COUNT          427 a          559 c          348 g          603 t
ORIGIN      
        1 tcggcgttgt attaaaaaca gcttccgcgc cccgtccctg tgttaaattt tttataaatt
       61 ccaccgggtc cttttcaagc ggattcaagg taatatcaca gccaagttct tttgcgatcc
      121 ttcttctgtc cgcatccggc tcgcttaaaa tcaccctcgc accgctcagc tttgccccaa
      181 gtatatggag catccccata atacctccgc cgatcactac cacatcatct ccaagttcga
      241 tcctgccttt ttcaatactg ttcagtacgc atgccagcgg ctctgcaaaa acagccgttt
      301 tatatgaaag ctcttccggt atctgatatg cctgtgatac gttgatcgtc aaatattctc
      361 caagtccgcc cgtacccgga atctccatct cggaattatc cagattgttc agttccacgc
      421 agagattttc ctctcctctg cggcagaaat aacactttcc gcagacattg atcagccttg
      481 ccactatttt actgccgacc ggaaattccg tttccctgac acctgctccc aatgccgcca
      541 cttcacctgc aatttcatgc ccgcctacaa acggaagggg catatgggat tctcttgtaa
      601 atgtcctctg ctcccatgtg cagatcgcac aggcattgat tttcatcaaa atctgtccgg
      661 gcttcggcga cggctttctg acttccctca cctcaacctt tcgttcatct gcaattacta
      721 ctactttcat cttctcgctc attacttatc tcctatcctg cagcagatat accacctgtt
      781 atttgccgta ataaaatttt ttatataatg cttccagttc ctcttggtcc ggatctttcg
      841 gtgcaaacat gatatttgca tcctgcaggg ccgcttctat atagagagga attcttttca
      901 gataatctgc ttccggaatc cctgcttcct gcagcgttat gggcatatgg agctttctgt
      961 ataactccca tatcctttct gtcaggatcc tctgcttttc ctcagactgc cctgttattc
     1021 caagctggtc ggctacctct tcatagatcg gctgtggtat cagatactcc attgtatagg
     1081 gtaagagcat cccggtcatc attccgtgcg gctttccaaa atcaccgcct gcctgatcca
     1141 ttccgtgtgc cattcctgta atggaattat tgattcctcc gcctgccatc gttgctgcga
     1201 tatgcacctt ttctctggct tccatatcgc cttccattga ctttggcaat tccttgatca
     1261 gggttacggc cgcctggatg cactgcatac gtaccattgt actcgccagc ctgcttgcgc
     1321 ctgcctcaag cgcatgagcc agcgcatccg ttccggaaaa agcaatgttt cctttcggaa
     1381 ggctcttcaa caagtcaaaa tccataatgg ctgtcgttgg gatgatctca ttgctcaata
     1441 tcatcctttt tttcttctgc gcatctttca tgaccgcaca gcctgttgtc tcagaaccgg
     1501 tcccgcttgt tgtaggaaca acgatcaatt ttgcctttcc cggaaaaggt gccacttcat
     1561 atgctttaaa agccttctcc caatcatact ccggcaattc ataaagcagc cataaagctt
     1621 ttgcagtatc catcacagaa ccgccgccga ttcctacgat gatatccggc ccgaatgcac
     1681 ggacctcctc aatcggacct tgcagcattt ccatatgggg ctctgttctc atgtcgcata
     1741 gtattctgta atccgagtcc ttcagcagat tttcaaacaa gatctgtttt aaatgcaatg
     1801 cttccagaat tccgccgtca ataacaaatg caagcttttt atccttatat tcacttaatg
     1861 tttcaatact tccgtttcca agtacatatt gtgtagttct gtatgcggca tactgcatgc
     1921 ttcattcctc ctgtttc
//
LOCUS       DAOSYE010000449         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_49468_length_1933_cov_4.152822, whole genome shotgun sequence.
ACCESSION   DAOSYE010000449 DAOSYE010000000
VERSION     DAOSYE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49468_length_1933_cov_4.152822"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1933
                     /locus_tag="PLU43_07955"
     CDS             159..>1933
                     /locus_tag="PLU43_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOO28385.1"
                     /translation="MGETENLKLWYKKPARKWEEALPIGNGRLGGMVYGRIYSEMIQL
                     NEDSVWYGGAVDRNNPDAYRNLSKIRQLIFDGKIREAEELTAFALSGIPESQRHYEPL
                     GNLCLFFANQKGNVQNYKRELALCRGTVTTEYQLDGIGYKREIFASYPQQIMVIRLTA
                     DRPGAISFHTQLARGNELWGDEPVGTGKLWYSGGFNAFVDRSCAIGSDGSMMSGQCGG
                     KGAVEFCGRIKVKAEGGQVKTIGNSVVVNAADAAVIYLAAETTFRETDAEGACAKRIK
                     QAEEYSYEKLLAAHLKDYEELFGRVRIDVQSSRDRTIDSLPTDERMKRHSLGEEDQGL
                     INLFFQYGRYLLIASSRPGSLPANLQGLWNREMLPAWDSKYTININTQMNYWPAETCN
                     LSECHVPLFDLIERMRAPGRKTAELMYGCRGFMAHHNTDIWADTAPQDVCLSSTYWMM
                     GAAWLCLHLWEHYEFTKDAGFLSGVYETMREAALFLVDFLIEDADGYLVVCPSISPEN
                     EYRLNNGETGVLCKGASMDSQIIYALFHACIRASELLDRDLAFRQELELLLKKIPEPQ
                     IGKYGQIMEWKEDYEELDPGHRHIS"
BASE COUNT          575 a          359 c          534 g          465 t
ORIGIN      
        1 tccatatcgt ttatgtatga ccggccggaa agtattcgaa attataaaaa tattgtaata
       61 atcgtctgcg gcggctcggt aatcaatctg gagaaaatac tggagtggaa atccaaatat
      121 caaattgaat aataaacagt gataaaaggg agatttttat gggtgaaaca gaaaatttaa
      181 agctatggta taaaaaaccg gcaaggaaat gggaagaagc cctgccgatc ggcaatggac
      241 ggcttggcgg catggtatac ggaagaattt acagtgaaat gatccagctg aatgaagaca
      301 gtgtatggta tggaggtgct gttgaccgaa acaatccgga cgcgtaccgg aatctttcaa
      361 aaatacggca gctgattttt gacggaaaaa tcagggaagc agaagaactg actgcatttg
      421 cactttccgg tataccggaa agtcagcgtc attatgaacc gttgggaaat ctctgtttat
      481 tctttgccaa tcagaaagga aacgtgcaaa attataaaag agagcttgct ctttgccgcg
      541 gaacagtaac gacggaatat cagctggatg gaatcggcta taaacgtgag atctttgcaa
      601 gctatccgca gcagatcatg gtgatcaggc tgacagcgga ccgtccggga gcaatttctt
      661 ttcacacaca gcttgcaagg ggaaatgagt tatggggaga tgaacctgtc ggtacgggca
      721 aattgtggta cagcggaggg tttaatgctt ttgtggatag atcctgtgcc ataggaagcg
      781 atggttccat gatgagcgga caatgcggcg gaaaaggagc ggttgaattc tgcggaagga
      841 taaaagtaaa ggcagaagga ggacaggtca aaacaatagg gaatagtgtt gttgtaaatg
      901 ctgcggatgc ggctgttatt tatctggcag cggaaacgac attcagagaa accgatgcgg
      961 aaggagcatg tgcaaaaaga ataaaacagg ctgaggaata ttcttatgaa aaactgctgg
     1021 ctgcccatct gaaagattat gaggaattat tcggacgtgt ccggattgac gtgcaaagca
     1081 gcagagaccg gacgatcgac agcctgccga cagatgagcg tatgaaacgt cattctttgg
     1141 gtgaagaaga tcagggactg atcaatctgt tttttcaata cggccgttac ctgctgattg
     1201 caagcagcag acccggttct ttgccggcaa atctgcaggg gctttggaac agggagatgc
     1261 ttcctgcctg ggacagtaaa tatacgatca acatcaatac gcagatgaat tactggccgg
     1321 cggaaacatg taatctctcc gagtgtcatg ttcccttatt tgatctgatt gaaagaatgc
     1381 gtgctccagg cagaaaaact gcagagctca tgtatggctg ccggggattt atggcgcacc
     1441 acaatacgga tatctgggcg gatacagcgc cgcaggacgt atgcttaagt tctacatact
     1501 ggatgatggg agcggcatgg ctgtgccttc atctttggga gcactacgag tttacaaaag
     1561 atgccggatt tttaagcggt gtttatgaaa caatgcggga agccgctttg tttttagtgg
     1621 attttttgat agaagatgcg gatggatatt tggttgtctg tccgtcgatc tcaccggaaa
     1681 acgaatatcg gctcaataac ggagagaccg gcgtgctctg taaaggtgct tccatggaca
     1741 gtcagattat atatgccctg tttcatgcat gtatccgggc atccgagctg ctggaccgtg
     1801 accttgcatt tcggcaggaa ttagagctgc tgctgaaaaa aataccggaa ccgcaaatcg
     1861 gaaagtacgg acagatcatg gaatggaaag aagattatga ggagctggat ccgggacacc
     1921 ggcatatttc cca
//
LOCUS       DAOSYE010000450         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_49643_length_1929_cov_3.761473, whole genome shotgun sequence.
ACCESSION   DAOSYE010000450 DAOSYE010000000
VERSION     DAOSYE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49643_length_1929_cov_3.761473"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="PLU43_07960"
     CDS             <1..104
                     /locus_tag="PLU43_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOO28386.1"
                     /translation="ARMREYLYSLTNNEDLQTTVLPIGDGMTISVKK"
     gene            115..1359
                     /locus_tag="PLU43_07965"
     CDS             115..1359
                     /locus_tag="PLU43_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOO28387.1"
                     /translation="MGRNKKPELLIPASSLEVLKTAVVFGADAVYIGGEAFGLRAKAK
                     NFSREDMMEGIAFAHARHVKVYITANILAHNADLPAAEAYFKELSQIKLDALIISDPG
                     MFLLAKEFCPETEIHISTQANNTNYRTYEFWWKQGARRVVSARELSLKEIREIRERIP
                     KEMEIESFVHGAMCISYSGRCLLSSFFTGRDANRGACTHPCRWKYAVVEETRPGEYLP
                     VYENDRGTYIFNSKDLCMIEYLPEMLDAGIDSFKIEGRMKTALYVATVARTYRKAIDD
                     CFSSEALYRANMSWYKEEISKCTYRQFTTGFYFGKPDETTQIYDNNTYIKEYIYLGIA
                     EQVEEDGTITLRQKNKFSVGEEIEVMKPDGTNVKTAVLSITDEDGTEQESAPHPQQLI
                     HVRLKENLADVYDILRKKEESE"
     gene            1356..>1929
                     /locus_tag="PLU43_07970"
     CDS             1356..>1929
                     /locus_tag="PLU43_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28388.1"
                     /translation="MKLLTVIVPCYNEEQTVPLFYEEMKKTMEVFKTEFPETAFELLF
                     IDDGSGDGTMECIRALRKKDECVHYVSFSRNFGKEAAIYAGLSKAHGDHVVLMDADLQ
                     DPPSLLPQMYTAVTKEGYDSAATRRVTRKGEPRIRSFFARCFYKLINRLSETEIVDGA
                     RDYRIMTRQMVEAVLSMGEYNRFTKGIYGWV"
BASE COUNT          586 a          354 c          523 g          466 t
ORIGIN      
        1 atgcgcggat gagagagtat ctttacagtc tgacaaataa tgaagatctt cagacgacag
       61 tgcttccgat cggggacggc atgacgatca gcgtaaagaa atgaggaatc cgttatggga
      121 agaaataaaa agccggaact gctgatacct gcgagcagtc ttgaagtatt aaaaactgca
      181 gttgtttttg gtgcggatgc ggtatatatc ggcggtgagg catttggcct tcgggcgaaa
      241 gcaaagaatt tttcaagaga agatatgatg gaaggcattg catttgcgca tgcccgccat
      301 gtgaaagtat atataacggc aaatattctg gcacataatg ccgacctgcc ggctgctgaa
      361 gcgtatttta aagagctttc tcaaataaag ctggacgcgc tgatcatttc ggacccgggg
      421 atgtttctgc ttgcaaagga gttttgcccg gagacggaaa tccatatcag cacacaggca
      481 aacaatacga attacaggac gtatgaattc tggtggaaac agggagccag acgggttgtt
      541 tccgcaagag aactgtcttt aaaggaaatc agggaaatca gagaacggat tcctaaagag
      601 atggagatcg agagttttgt tcatggagca atgtgcattt cctattccgg ccggtgtctg
      661 ctcagcagct tttttacggg aagagatgcc aaccggggag cctgtacgca tccatgccgc
      721 tggaaatatg cggtcgtgga agagacccgg ccgggtgaat atctgccggt ttatgaaaat
      781 gacagaggca cgtatatttt taattccaag gatctgtgta tgatcgaata cctgccggaa
      841 atgcttgatg ccggtattga cagctttaaa atagaaggac gtatgaagac ggcgctgtat
      901 gtggcgactg ttgcaagaac ttaccggaaa gcgatcgatg actgtttttc gtcggaggca
      961 ttgtaccgtg cgaatatgtc ctggtacaaa gaagaaattt caaaatgtac atacagacaa
     1021 tttacgacag gattttattt tgggaaaccg gatgaaacga cgcagatcta tgacaataat
     1081 acgtacatca aagaatatat ttatctggga atcgctgaac aggtcgaaga agatggaacg
     1141 atcactcttc ggcagaagaa taaattttct gtaggggaag agatagaagt gatgaagccg
     1201 gacgggacga atgtgaagac cgctgtgctg tctattacag acgaggacgg gacggagcag
     1261 gaaagtgccc cgcatccgca gcagctcatc catgtcagat taaaggagaa tcttgcggac
     1321 gtatatgata tattaagaaa aaaggaagaa tcagaatgaa actgcttacc gtaattgtac
     1381 catgctataa tgaagaacag acagtgcctc ttttttatga agaaatgaaa aaaacaatgg
     1441 aagtattcaa gacggagttt ccggagactg cttttgagct ccttttcatc gatgacggtt
     1501 caggggacgg aacaatggag tgcatcagag ctctgcggaa aaaagacgaa tgtgtgcatt
     1561 atgtttcctt ttccaggaac tttggaaagg aagctgccat ttatgccggt ttaagcaagg
     1621 cacatggaga tcatgtcgta ttgatggatg cagatctgca ggatccgcca agtcttttgc
     1681 cccaaatgta tacggccgtg acaaaggaag gatatgattc ggcagctaca agacgggtga
     1741 caagaaaagg ggagccgcgg atccgttcgt tttttgcaag atgtttttat aaactgatca
     1801 accggctttc agagacggag atcgtagacg gagcacgtga ttaccggatc atgacaagac
     1861 agatggtgga agcggtcctt tccatgggag aatataaccg ttttacaaaa ggaatctatg
     1921 gctgggtcg
//
LOCUS       DAOSYE010000451         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50067_length_1919_cov_2.421674, whole genome shotgun sequence.
ACCESSION   DAOSYE010000451 DAOSYE010000000
VERSION     DAOSYE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50067_length_1919_cov_2.421674"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLU43_07975"
     CDS             <1..340
                     /locus_tag="PLU43_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOO28389.1"
                     /translation="ERAGLNTLMGMGTVFAVLILLCLIISCFKFIPRIQASFGKDKKT
                     KIETTKNESVDNTIAQIIEKEELTDDLELVAVITAAIAAAGTAGTAGGTDGFVVRSIK
                     RVSGSRWNQA"
     gene            365..727
                     /locus_tag="PLU43_07980"
     CDS             365..727
                     /locus_tag="PLU43_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO28390.1"
                     /translation="MKNYTITVNGNVYDVTVEEGASTGAAVAAAPKAAPKAAPKAAPK
                     AAAGAGSVKVEAGAAGKVFKIEANVGQAVKKNDTIVILEAMKMEIPMVAPQDGTVASI
                     DVAVGDSVEAGTVLATLN"
     gene            773..>1105
                     /locus_tag="PLU43_07985"
     CDS             773..>1105
                     /locus_tag="PLU43_07985"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOO28391.1"
                     /translation="MSYVANTMGNLLHQTAFFGLTGGNFVMILVALLFLYLAIRKGFE
                     PLLLCPIAFGMLLVNIYPDIILSIEESTSGTGGLLYYFYLLDEWAILPPLIFMGVGAM
                     TDFGPLIAN"
     assembly_gap    1106..1115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1116..1917
                     /locus_tag="PLU43_07990"
     CDS             <1116..1917
                     /locus_tag="PLU43_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOO28392.1"
                     /translation="GATAQFGIFTAYFGAIALGFNDKAAAAISIIGGADGPTSIFLAG
                     KLGQTALLGPIAVAAYSYMSLVPIIQPPIMKLLTTEKERKIKMEQLRPVSKLEKILFP
                     IIITIVVCLILPTTAPLVGMLMLGNLFRESGVVRQLTETASNALMYIVVIMLGTSVGA
                     TTSAEAFLNLDTLKIAGLGLFAFIFGTAAGVLFGKLMCLLTHGKVNPLIGSAGVSAVP
                     MAARVSQKVGAESDPTNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGIF"
BASE COUNT          528 a          360 c          503 g          518 t
ORIGIN      
        1 ggagagggca ggcctgaata cattgatggg aatgggaacg gtttttgccg ttttgattct
       61 gttatgtctg attatcagct gctttaagtt tattccaagg atccaggctt ctttcggtaa
      121 agacaagaaa acaaaaatcg aaacgacgaa gaacgaatcc gtggataata cgattgcaca
      181 gattatagaa aaagaagaac tgacggatga tctggagctt gtggcagtga tcaccgcagc
      241 aatagccgca gcaggtacgg caggaacagc cggtggaact gacgggtttg ttgtaagaag
      301 tattaaaaga gtatccggta gtagatggaa tcaagcataa ttataaaaca ggaggattat
      361 taaaatgaag aattatacaa ttaccgtaaa tggcaacgta tatgatgtaa cagtagaaga
      421 aggagcatca accggagcag ctgtggcagc agcaccgaag gcggcaccga aagcagctcc
      481 aaaagcggca ccgaaagcag ctgcaggcgc aggaagtgta aaggtagaag caggcgcagc
      541 cggaaaagta tttaagatcg aggcaaatgt cggacaggct gttaagaaga acgatacgat
      601 cgtcattctt gaagctatga agatggaaat tccgatggtc gctccgcagg acggcacggt
      661 tgcaagtatt gacgtagctg ttggagacag tgttgaagcc ggtacggttc ttgcaacatt
      721 gaactgatcg attggcatat gtagtgaaaa agaataacat ggaggtcata aaatgtcata
      781 tgtagcaaac actatgggaa atctactcca tcagacggcg tttttcggct tgaccggcgg
      841 aaatttcgtt atgattcttg tggcattact tttcctgtat ttagcaataa gaaaaggatt
      901 tgagccattg ctgctttgtc cgattgcttt tggtatgctg cttgtaaata tctatccgga
      961 tattatttta tcgatagaag aatcgacgag cggtacgggt ggtctgcttt attatttcta
     1021 tttattagat gaatgggcaa tccttcctcc ccttattttc atgggggttg gagcaatgac
     1081 ggattttggt ccgttgattg caaatnnnnn nnnnnaggtg cgacagcaca gttcggtatt
     1141 tttacagcat attttggtgc gatcgcgctt ggttttaacg ataaggcagc tgctgctatt
     1201 tccatcattg gtggtgcaga cggccctaca tcgattttcc ttgcaggcaa gcttgggcag
     1261 acggccctgc ttggtccgat cgcggtagcg gcatattcct atatgtctct tgtgccgatc
     1321 attcagccgc cgatcatgaa attgctgaca acggaaaaag aaagaaaaat taaaatggaa
     1381 cagctgcgtc cggtttctaa actggaaaag atccttttcc cgatcatcat cacgatcgtc
     1441 gtatgtttga ttcttccgac aacagcaccg cttgtaggta tgctgatgct tggtaatctg
     1501 ttcagggaat ccggtgttgt cagacagctg acggaaacag cttccaatgc tttaatgtat
     1561 atcgtagtta ttatgcttgg tacatctgtt ggtgcaacaa caagtgcaga agcatttttg
     1621 aatctggata cactgaaaat tgctggctta ggtctgtttg catttatatt tggtacggcg
     1681 gcaggcgttc tgttcggcaa actgatgtgt ctgctgactc atggaaaggt gaacccgtta
     1741 attggttctg caggcgtatc ggctgtgcct atggcagcca gggtatcaca gaaggtcggg
     1801 gcggaatcgg atccgacgaa cttcttactg atgcatgcca tgggaccaaa cgtagccggt
     1861 gttattggaa ctgctgttgc agcaggaaca ttcatggcga tattcggtat attctaagc
//
LOCUS       DAOSYE010000452         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50110_length_1918_cov_2.685454, whole genome shotgun sequence.
ACCESSION   DAOSYE010000452 DAOSYE010000000
VERSION     DAOSYE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50110_length_1918_cov_2.685454"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLU43_07995"
     CDS             complement(<1..943)
                     /locus_tag="PLU43_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28393.1"
                     /translation="MERGMDTVAIVVVAYNRPDSLSRLLMSLAAADYPEDKEIPLIIS
                     IDKGNNAEVIRAADNFAWIHGEKSVVCRDTNLGLKQHVLLCGDYTKIYGNIIMLEDDL
                     YVSPAFYYYAQAALAFSDTQEDIGGISLYNHQLNVHVREPFAAIHDGYDNWYFQFASS
                     WGQAFGSRQWSAFLEWFQENGKKPLASANIPENVSSWSDRSWLKYYIKYLVESGKYFL
                     YPQVSLTTNFSEEGVHAAKTVTDLQVPLAGRIIQNPSYRFSSLQESGAVYDAFFENIC
                     LKKQAAAQLAEMPEEYCKEEKIEIDLYGYKPIGSKVRY"
     gene            complement(983..1678)
                     /locus_tag="PLU43_08000"
     CDS             complement(983..1678)
                     /locus_tag="PLU43_08000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOO28394.1"
                     /translation="MLKRKLEEAGSGLYRSLIYPFVRIGIEKRTHSIMKQKSYLNKGS
                     VLNGKNYIGKRVVLSHTTVGFGSYINDDGNFSNTVIGKYTSVGTDVSVLLGRHPVGKF
                     AAMHPAFYSTGAALGYSYAARDSFEEFDYVDKERGIQIVIGNDVWIGSGVRIMEGVTI
                     GDGAVIGAGALVLTDVEPYAVVAGVPAKKIRMRFSGEQIEKLLTLQWWNKGEAWIREH
                     AEAFCSVEELLRD"
BASE COUNT          532 a          512 c          340 g          534 t
ORIGIN      
        1 gataccgcac ttttgatccg atcggtttat atccatataa gtctatttcg atcttttcct
       61 ctttgcagta ttcttccggc atttctgcaa gctgtgcagc agcctgtttt ttcagacaga
      121 tattctcaaa aaaagcgtca taaacagctc ctgattcctg cagagaactg aaccgatagc
      181 tgggattttg tatgatcctt cccgcaagcg gcacctgcag atccgtaact gtttttgccg
      241 catgcacacc ttcctcagaa aaattcgtcg tcagggaaac ctgcggatat aaaaaatact
      301 ttccggattc cacaagatat tttatataat atttcagcca tgaccgatca gaccaggaag
      361 aaacattttc cggaatatta gcagaagcaa gcggcttttt gccgttctcc tgaaaccact
      421 ctaaaaaagc actccactgc ctgctgccaa atgcctgtcc ccaggaagag gcaaactgaa
      481 aataccagtt atcatatccg tcatgaattg cagcgaacgg ttcccgtaca tgtacattca
      541 gctgatgatt ataaagggaa attccaccga tatcctcctg cgtatctgag aaagcaagtg
      601 cagcctgtgc ataatagtaa aatgcgggag aaacatacag atcatcctcc agcataataa
      661 tattcccata aattttcgta tagtctccgc ataaaagaac atgctgcttc agcccaagat
      721 ttgtatccct gcaaacgacc gacttttcac catgtatcca tgcaaaattg tcagcggccc
      781 gaatgacttc ggcgttgttt cctttatcaa tactgatgat cagcggtatc tctttgtcct
      841 ccggataatc cgccgctgcc aaagacatca gcagccggct cagtgaatcc ggtctgttat
      901 acgcaacaac tacgatcgca actgtatcca tcccacgctc catttatcat atctgttcgt
      961 ttatatttca aaagaccgtt acttagtccc gcagcagctc ttccacgctg cagaacgctt
     1021 ccgcgtgctc cctgatccat gcttcccctt tattccacca ttgcagggtc aaaagttttt
     1081 cgatctgttc accggaaaaa cgcatcctga tcttctttgc cggaacgcct gcaactaccg
     1141 catacggttc cacatctgtc aatacaagag cccctgcacc gatgaccgca ccgtctccga
     1201 tcgtaacgcc ttccatgatc ctgacaccgc ttccgatcca cacgtcattc ccgattacga
     1261 tctgaattcc ccgctcttta tccacataat caaactcctc aaaggaatca cgtgctgcat
     1321 aactatatcc gagtgccgca cctgtcgaat aaaaagcagg atgcatagcc gcaaacttcc
     1381 cgacaggatg tctgccgagc agaacggaaa catccgtccc aacagacgta tatttcccaa
     1441 tgacggtgtt ggaaaaattg ccgtcgtcat taatatagga accaaagccg actgtcgtat
     1501 gcgacagcac cacccgtttt ccaatatagt tttttccgtt aagaaccgag cccttgttca
     1561 aataggactt ctgtttcata atactgtgcg tccttttttc gatcccgatt ctgacaaacg
     1621 gatagatcag gctgcggtat aatccggaac cggcttcctc aagctttctc ttcagcatct
     1681 gcttctctct tttgtcttat tttcatacta tgcgcaaata cataattcag tataaatcaa
     1741 tttccctaaa agcacaatac aaaagcaccc ttgagttgac ttcctgcctc cgaaccttta
     1801 atataagaaa taactttatt gctgtttatt tatagaaaaa cgaaggaacg atccgtatga
     1861 aattcgttac tcttttttta aaaaccgaga atgtacatct gctgaaagat gtcggtat
//
LOCUS       DAOSYE010000453         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50227_length_1915_cov_3.361828, whole genome shotgun sequence.
ACCESSION   DAOSYE010000453 DAOSYE010000000
VERSION     DAOSYE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50227_length_1915_cov_3.361828"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLU43_08005"
     CDS             <1..720
                     /locus_tag="PLU43_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO28395.1"
                     /translation="YLLASKELFCAQAKKLSYALLREERANNLINNMRFANKTFGGFL
                     VGKILDSLIIGIICFVGTSILKIPYALLISVIVGVTNMIPFFGPYLGAIPCAIILIMI
                     NPMKCLIFLIFILILQQFDGNILGPKILGNSTGLSSFWVIFAITIFGGLLGILGMFIG
                     VPLFAVIYSAIKTFVNQRLIKKQLPADTTFYMGSDYHSKETDMNTNSGTEIKFVKKAF
                     DHVVAEGTTAESQSSETNKES"
     gene            723..1178
                     /gene="dtd"
                     /locus_tag="PLU43_08010"
     CDS             723..1178
                     /gene="dtd"
                     /locus_tag="PLU43_08010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOO28396.1"
                     /translation="MKFVIQRVKQASVKANGQLTGTIEKGYLILIGVCDADTTATADK
                     MIKKTAALRIFQDENGKTNLSVKDVNGALLLVSQFTLYADCKKGNRPSFIKSGSPEHA
                     NALYEYIIHEFSFSYDLPVQTGIFGADMDVSLINDGPFTIMLDSEELFA"
     gene            1422..>1915
                     /locus_tag="PLU43_08015"
     CDS             1422..>1915
                     /locus_tag="PLU43_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOO28397.1"
                     /translation="MELQRLLSYTRKAVDDYQMIDSGDKIAVGISGGKDSLTLLYALH
                     GLRRFYPNKFELEAITVNLGFPDFDLGPVKELCHQLDIRYTVVDTEIYQIVFQERKET
                     NPCSLCAKMRKGALNEAIKALGCNKVAYAHHKNDIVETMLLSLIFEGRFHSFSPKTYL
                     DRMDL"
BASE COUNT          578 a          362 c          398 g          577 t
ORIGIN      
        1 tatctgctgg caagcaagga attgttctgt gcgcaggcca aaaagctctc ttatgcgctt
       61 ttaagagaag agagagctaa taacctcatt aacaatatgc ggtttgccaa caagacattt
      121 ggaggctttt tagtcgggaa gatactggac tcactgatta ttgggattat ctgttttgtc
      181 ggaacaagta tcctgaaaat tccatatgcg cttttaatca gcgtaatcgt gggcgttaca
      241 aatatgattc cttttttcgg tccttacctt ggtgcaatac catgtgcgat catactgatt
      301 atgatcaatc cgatgaaatg tctgatattc ttaatattta tattgatcct gcagcagttt
      361 gacggaaata ttcttggtcc gaagattctt ggaaattcta ccggattatc aagcttttgg
      421 gttatttttg caattacgat attcggcgga cttttaggta ttcttggtat gttcatcggc
      481 gttcccctgt ttgcggttat ttattccgcg atcaaaacat ttgttaacca acggctgata
      541 aaaaaacagc ttcctgcaga tacaacgttt tatatgggat ccgattatca ttcaaaagaa
      601 acagatatga atacgaattc tggcacggag atcaaatttg taaaaaaagc atttgaccat
      661 gtcgttgccg aaggaacaac agcagaatcg caatcatcag aaaccaataa ggaatcgtaa
      721 ctatgaaatt tgtcattcaa cgagtgaaac aggcctcggt aaaagcaaac ggacaattga
      781 ccggtactat tgaaaaagga tatttgatac tgatcggtgt ctgtgatgca gatacaacgg
      841 ctacagctga taaaatgatc aaaaaaaccg cagctttgcg tatatttcag gatgaaaacg
      901 gaaaaacaaa cctttccgtc aaggatgtaa acggggcctt gcttcttgtt tcgcaattta
      961 cactttatgc ggactgtaaa aagggaaacc gtccatcctt tattaaatcc ggcagtccgg
     1021 agcatgccaa tgcgctttac gagtatatta ttcatgaatt ttctttttcc tatgacctgc
     1081 cggtacaaac cggcatattt ggtgcagata tggatgtttc gcttatcaat gacggaccgt
     1141 ttacaatcat gcttgattcg gaagagttat ttgcctgaag ggtattacac tttcatgcat
     1201 tttgatgttt cgatggaaat ataaatgaat tagaattttc gaaccaatga aaaaggagaa
     1261 gaaactgaac gaaacagtac gctcaaaagg agccaaaagg tgaaacttgc cggcgtagcc
     1321 ctaacgtcct aagaacagcc aatctattcg ttaaagttgt ctttcgcgaa tagatatgtt
     1381 tctctgcttc caggtgatgt ttcgtttatt tcacaatgac aatggaatta cagcgtttat
     1441 tgagttatac acgcaaggcc gtggacgatt atcaaatgat cgactccggc gataaaattg
     1501 cagttggtat ttccggcggc aaagacagtc tgaccctgtt atatgcttta catggtttac
     1561 gccgctttta tccgaataaa tttgagctgg aagcgatcac tgttaacctt ggctttccgg
     1621 actttgatct gggtcctgtc aaagaacttt gtcatcagct ggatatccgt tatacggtcg
     1681 tagacacaga aatctatcaa atcgtttttc aggaacgcaa agaaacaaat ccctgttctt
     1741 tatgcgctaa aatgcgaaaa ggggctctga atgaagcgat caaggctctt ggctgcaata
     1801 aagtagcata tgcacatcat aaaaatgata ttgtggaaac aatgctgcta tctttaattt
     1861 ttgaaggaag atttcattct ttttcgccaa aaacttattt agaccgcatg gacct
//
LOCUS       DAOSYE010000454         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50241_length_1915_cov_2.516129, whole genome shotgun sequence.
ACCESSION   DAOSYE010000454 DAOSYE010000000
VERSION     DAOSYE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50241_length_1915_cov_2.516129"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>820
                     /locus_tag="PLU43_08020"
     CDS             94..>820
                     /locus_tag="PLU43_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO28398.1"
                     /translation="MYEYYNYENQVIPNGREQQEAERKANAELEKKNAKKARRKKWTS
                     CVAYGLVFGIVAGGAFQGVNYIGNKITKSNDVIAQAPADEQVEAQTAQEETTTDDTAT
                     APAKTVSTTDDSDATMVVADVAADAMPSIVSITNKSVQEVQMMFGMGTQEYESESCGS
                     GIIVGKNDEELLIMTNNHVVEGANTLSVGFIDNEVYEAAVKGTDSENDLAVIAVKLSD
                     IKDSTMEQIKVATLGDSSDLVIGE"
     assembly_gap    821..830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <831..1590
                     /locus_tag="PLU43_08025"
     CDS             <831..1590
                     /locus_tag="PLU43_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO28399.1"
                     /translation="VVAIGNALGYGQSVTTGIISALDREVSIENLTSKLIQTDAAINP
                     GNSGGALLNMNGEVIGINSAKFASSQVEGMGYAIPISTATPIIEELMNRQTRELVDES
                     EASYIGITGLSVTSEVSDTYGIPEGIYVSEVGENTPAEEAGLLKGDVIKKFDGTSVMS
                     ITELQEQLKYYKAGETVDMLIARADNGEYKETTITITLGEKANSDYAQSGSQDESSDD
                     NSTDNGSSGSSGGDLKDFLPQNSTGENILQFFGY"
BASE COUNT          623 a          326 c          511 g          445 t
ORIGIN      
        1 ctaaaaaagc agataaagca ggccgtgttg gctgcacaaa aaggcggttt tatcagatgc
       61 aggattatag aatagaaagt gaggatgaag atcatgtacg aatattataa ttatgaaaat
      121 caggtcatcc ctaatggaag ggagcagcag gaggctgaaa gaaaggcaaa tgcagagctg
      181 gaaaagaaaa atgccaaaaa ggcaagaaga aagaaatgga cgtcatgtgt tgcttacgga
      241 cttgtttttg gtattgttgc aggaggcgca ttccagggcg tcaactacat tggaaataaa
      301 ataacaaaaa gcaatgacgt gatagcacag gcaccggcgg atgaacaggt agaggcgcag
      361 acagcacagg aggaaacaac gacagatgat acagctaccg ctcctgctaa aacagtcagt
      421 acaacagatg acagcgatgc gacaatggta gtggcggatg tggctgcaga tgcaatgccg
      481 tccatcgtat ccattacaaa caagagtgtt caggaagtac agatgatgtt cggtatggga
      541 acacaggagt atgaaagcga aagctgcggt tccggtatta ttgtcggcaa gaatgacgaa
      601 gaacttttga tcatgaccaa taatcatgtt gttgagggtg ccaatacact gagtgtagga
      661 ttcattgata acgaagtata tgaagcagct gtaaaaggta ctgattcgga gaacgatctt
      721 gcagtgatcg ccgtaaagct ttccgatatc aaagacagta cgatggagca gatcaaagtt
      781 gcaacacttg gtgattccag tgatcttgtg atcggggaac nnnnnnnnnn ggtcgttgca
      841 atcggtaatg cactcggata tggacagtcg gttacgaccg gtattatcag tgcacttgac
      901 agagaagtca gtattgagaa tctgacaagt aaactgatcc agacggacgc agcaatcaat
      961 cccggcaaca gcggcggagc tcttttgaat atgaatggtg aggtcatcgg tatcaattca
     1021 gccaagtttg cttcttcaca ggttgaaggt atgggttatg cgatcccgat ctccacggca
     1081 acaccgatca ttgaagagtt aatgaacagg cagacgagag aactcgtaga tgaaagcgag
     1141 gcaagctata tcggaattac cggactttcc gttacaagcg aggtttcgga cacctatggt
     1201 attccggaag gtatttatgt atccgaggta ggagaaaata caccggcgga ggaagcagga
     1261 cttctgaaag gtgatgttat aaagaaattt gacggtactt cggttatgag cattacggaa
     1321 ctgcaggagc agctgaagta ttataaagca ggtgaaaccg ttgatatgct cattgcaaga
     1381 gcagataacg gggaatacaa agaaacgacg atcactatta ccctcgggga aaaggcaaac
     1441 tcagactatg cacagtccgg cagtcaggat gaaagcagtg atgacaacag cacggataac
     1501 ggaagctccg gcagcagcgg gggagattta aaagattttc tgccgcagaa cagcactggt
     1561 gaaaatatcc ttcagttttt tggatattga tctgatattg tattgatcag tcatataaaa
     1621 taaatacgga aatacagaga agaagacaga ggatccaaag gcgggtcctc tgcctttttg
     1681 ctgttttgca ataattttct tgtattggag aaaaagtatg ttaaaataaa actgtcaatc
     1741 aatgttattt gcatagcggg acctgggaac aggagactat gtagtgaaga acagaataaa
     1801 atggattttg gcagtgtttt ttttggtatt ttttcttttg accggctgtg atgaagcagg
     1861 agaggaacag ttgcttcttt tagaggaaga gatacaggaa gagccagtcg tggca
//
LOCUS       DAOSYE010000455         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50264_length_1914_cov_3.764927, whole genome shotgun sequence.
ACCESSION   DAOSYE010000455 DAOSYE010000000
VERSION     DAOSYE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50264_length_1914_cov_3.764927"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLU43_08030"
     CDS             <1..343
                     /locus_tag="PLU43_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOO28400.1"
                     /translation="DTVTGKIELVMKADREHVPIICSMGAGNKLEASLFEVADIYKTS
                     VCPLARVMRRELKARGIGGCKVVYSKEQPKKPVYADTGRREEQFPASNAFVPAAAGLI
                     IAGEVVKDLCG"
     gene            complement(411..483)
                     /locus_tag="PLU43_08035"
     tRNA            complement(411..483)
                     /locus_tag="PLU43_08035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:Lys,seq:ctt)
     gene            581..1348
                     /locus_tag="PLU43_08040"
     CDS             581..1348
                     /locus_tag="PLU43_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO28401.1"
                     /translation="MIFETHAHYDDPAFDGDRKELLSQMENNGIYRVVNVSSDLESCS
                     KTIELTKQYSFIYGAVGIHPSDSGDLTEEDICSIEEQSRHEKIVAIGEIGLDYYWDSP
                     AREVQKKWFVRQLALAAKRQLPVIIHSRDAAQDTYDIMKEQQAEKIGGVIHCFSYGPE
                     MAERYLKMGFYIGIGGVLTFKNARKLKEVAAMLPLDSIVLETDSPYLAPEPNRGKRNS
                     SLNLSYIVEELAGIKRTSAAEVMCITTENAKRLYGMK"
     gene            1392..>1914
                     /gene="rsmA"
                     /locus_tag="PLU43_08045"
     CDS             1392..>1914
                     /gene="rsmA"
                     /locus_tag="PLU43_08045"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOO28402.1"
                     /translation="MASLGTPKNTAAIIQKYHFVFQKKYGQNFLTDTHVLEKIMKAAD
                     ITKEDYVIEIGPGIGTMTQYLCERARGVAAIEIDKMLIPILEETLSEFDNVTIVQADV
                     MKFDLVQLIKEKNDGKPVKIVANLPYYITTPIIMNLLENHTPIKSITVMVQKEVADRM
                     QVGPGTKDYGALSL"
BASE COUNT          624 a          344 c          489 g          457 t
ORIGIN      
        1 tgatacggta acgggaaaaa tagagcttgt tatgaaggcc gacagagagc atgtgccgat
       61 catctgttcc atgggggcgg gaaataaact ggaggcttcc ctgtttgagg tggcggatat
      121 ttataaaact tcggtctgtc cgcttgcaag ggtcatgaga agggaactga aggcaagggg
      181 aattggcggg tgtaaggtcg tttattccaa agaacagccc aaaaagccgg tttatgccga
      241 tacaggcagg cgggaggagc aatttccggc aagcaatgct tttgtaccgg cagcagcagg
      301 actgattatt gcgggggaag tcgtgaaaga tctgtgcggg taaaaagtat tccggaaaaa
      361 taataaagca ccaatcccat agcttaaatg ctacaaaatc aatgcttaga tgcgcgacca
      421 ggggttcgaa ccctggacac cctgattaag agtcaggtgc tctaccaact gagctagtcg
      481 cgctgattgg taacgcaatg actattatat tataatgtat ttggaaatgc aagtattttt
      541 aataaaaaat tgataaaaag caaaggattt cagaggaaaa atgatttttg aaacacatgc
      601 gcattatgat gatccggctt ttgacggaga tcggaaagaa ttgctttcgc agatggaaaa
      661 taacgggata tacagagtgg tgaatgtctc ttccgatttg gaaagctgca gcaaaacaat
      721 agagcttaca aaacagtatt cctttattta cggagcggtc ggtattcatc cgagcgacag
      781 cggggatctg actgaagagg atatttgcag catcgaagaa cagagcaggc atgaaaaaat
      841 tgtcgcgatc ggagagatcg gactggacta ttactgggac agcccggcac gggaagttca
      901 aaaaaaatgg tttgtcagac agttagcgct tgcagccaaa aggcagcttc ctgttattat
      961 tcacagcagg gatgcggcgc aggataccta tgatattatg aaagaacagc aggcggaaaa
     1021 aatcggcggt gtcattcatt gcttttccta tggccccgaa atggcagaaa ggtatttgaa
     1081 aatgggcttt tatattggga tcggcggtgt cctgaccttt aaaaacgcaa gaaagctgaa
     1141 ggaggttgcg gcaatgctgc cgcttgacag catcgttctt gagacagaca gcccctatct
     1201 tgcgcccgaa ccaaaccgcg gaaagaggaa ttcttctttg aatctttctt atatagtaga
     1261 agaattggcc gggataaaga ggacttcggc agcggaagtt atgtgcatta caacagaaaa
     1321 cgcaaagcgg ctgtatggaa tgaaataaag atttaataga aaaaagagcg tatacaccgg
     1381 aaaggaccag tatggcttca cttggaactc caaaaaacac ggcggcgatc atacaaaaat
     1441 atcattttgt atttcagaaa aaatacggac agaatttttt gacagataca catgtacttg
     1501 aaaagatcat gaaagctgcc gacatcacaa aagaggatta tgtaattgaa atcggaccgg
     1561 gaatcggtac gatgacacag tatttatgtg aaagagcgag gggcgtggca gcaattgaga
     1621 tagataaaat gctgataccg atccttgaag aaaccttatc ggaatttgat aatgttacga
     1681 tcgttcaggc cgatgtcatg aaatttgatc tggttcagct gatcaaagag aaaaatgacg
     1741 gaaaacctgt taagatcgtg gcaaatctac cttattatat tacaacaccg attattatga
     1801 atcttcttga aaaccatacg cctataaaaa gtattacggt tatggtgcag aaagaagttg
     1861 ccgacagaat gcaggtaggc cctggaacaa aagattacgg agcgctctcg cttg
//
LOCUS       DAOSYE010000456         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50266_length_1914_cov_3.479828, whole genome shotgun sequence.
ACCESSION   DAOSYE010000456 DAOSYE010000000
VERSION     DAOSYE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50266_length_1914_cov_3.479828"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1193)
                     /locus_tag="PLU43_08050"
     CDS             complement(198..1193)
                     /locus_tag="PLU43_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28403.1"
                     /translation="MKNQLMDILNVQEKEELRIYQSEEQLLEMIREDFTNGEAILQLL
                     KTSHAEAVRFMGKGTLNGFVGGAAIPVRKCDSFGIKKHTVCQVPYFHDHSFYEMIYVL
                     RGTCVQSFIGNPDTLTLYESQLCLLCPGTIHSIARCGTDDMILKITIPKKLFMDAVAP
                     VLKEDEDENPDCCFWPDVMVFKHVSARIDFYVTMLLEEILQKDRHSGSAIQNYISLLF
                     IELKRGTKPKASEILNRLHQYFKTDTLQPSLHGFAQTMGYSDAYTGRLLKEKTGKSFL
                     ELALKMKLEKAEKLLTETTLSVEEIAEKLSYANASGLYKQFYKCYGMTPNAYRKK"
     gene            complement(1206..>1914)
                     /locus_tag="PLU43_08055"
     CDS             complement(1206..>1914)
                     /locus_tag="PLU43_08055"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO28404.1"
                     /translation="DIADVEEILLKQVQYNSRISEEGLSGDYGANIGSTMLKVYGTDI
                     RNRAIAKAAAGADARMSGCELPVIINSGSGNQGITVSIPVIEYAKELGVPEEKLYRAL
                     IISNLTAIHEKTGIGTLSAYCGAVSAGCAAGCGIAYLYGGKYEELAHTLVNSVAIVSG
                     IICDGAKPSCAAKIASSVEAGILGYHMYMNGQEFKYGEGIVNNGVEKTIYNIGRLGKD
                     GMKQTDKEIVKIMLNQC"
BASE COUNT          459 a          509 c          331 g          615 t
ORIGIN      
        1 caccgatttc acagctttct ccgaaatgga taccggcaag ctcccgaatc ttttcatccg
       61 gcagccgatt cttcgtttta cttttcattg gccttctccc tgcaatcttt ttctgtttat
      121 tgtatcggaa aatacctttt gaaaaaatat ccccagacga ctgttttatt tccttataat
      181 acgaatcttc ttttctttta tttttttctg tacgcgtttg gtgtcatccc ataacattta
      241 taaaactgtt tatataatcc ggaggcattt gcataactga gcttctccgc aatctcttcc
      301 acggaaagag tcgtttccgt aagcagtttc tccgcttttt ccagcttcat cttaagagcc
      361 agctccagaa aactttttcc cgtcttttct ttaagaagcc tccccgtata ggcatcactg
      421 tatcccatcg tttgtgcaaa tccgtgcagg ctcggctgta aggtatccgt tttaaaatat
      481 tgatgcagcc ggttcagtat ttcggaagct ttcggctttg ttcctctctt caattctata
      541 aaaagcaggg atatgtaatt ttgtattgca ctcccggaat gccggtcctt ttgcagtatt
      601 tcttctaaaa gcatcgtaac ataaaaatca atgcgggcgg aaacatgttt aaaaaccatt
      661 acgtccggcc aaaaacagca gtccggattt tcatcttcgt cctccttcag cacaggggca
      721 acagcatcca taaacagctt ctttggtatc gttattttca gaatcatatc gtccgtaccg
      781 catctggcaa tagaatggat agttcccgga cacagcaggc acagctggct ttcatacaat
      841 gtcagtgtat cgggattccc gataaaagac tgcacgcatg tcccccgcag cacatagatc
      901 atttcataaa agctgtgatc atgaaaatac ggaacctgac aaaccgtatg tttttttatg
      961 ccgaacgaat cacatttcct gaccgggatc gccgctccgc ccacaaaccc gttcagcgtt
     1021 ccctttccca taaaccgcac tgcctctgcg tgtgaagtct ttaaaagctg caggatcgcc
     1081 tcaccgttag taaaatcctc ccgtatcatt tccaaaagct gttcttccga ctgatagatc
     1141 cttaattcct ctttttcctg tacgttcaaa atatccataa gctgattttt cataaacgtt
     1201 tcctttcagc attgattcag catgattttc acgatttcct tatctgtctg tttcattccg
     1261 tcttttccaa gacggccaat attatatatc gttttttcca ccccgttatt tacgatccct
     1321 tctccgtatt taaattcctg tccgttcata tacatatgat atcccaaaat gcccgcttct
     1381 acactggatg caatttttgc cgcgcaggat ggctttgccc cgtcacaaat aatgccggaa
     1441 acgattgcta cggaatttac cagtgtatgg gccagctctt catattttcc gccgtataaa
     1501 tatgcaattc cgcagcctgc cgcacatccg gcgcttactg ctccgcaata agccgaaagc
     1561 gtccctattc ctgttttttc atggatcgcc gttaaatttg aaataatcaa tgccctgtat
     1621 aatttttctt ccggcactcc aagctctttt gcatactcga ttaccggaat agacaccgtg
     1681 atcccctgat ttccgcttcc ggagtttata ataacaggca gttcgcagcc gctcatcctg
     1741 gcatcggcgc ctgctgccgc tttggcgatc gcccgatttc gtatatccgt tccatatacc
     1801 tttaacattg tagaaccgat attggctccg taatcacccg acagtccctc ttccgagatc
     1861 ctgctgttat attggacctg ttttaatagg atctcttcca catctgcaat gtca
//
LOCUS       DAOSYE010000457         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50345_length_1912_cov_3.617124, whole genome shotgun sequence.
ACCESSION   DAOSYE010000457 DAOSYE010000000
VERSION     DAOSYE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50345_length_1912_cov_3.617124"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="hrcA"
                     /locus_tag="PLU43_08060"
     CDS             complement(<1..722)
                     /gene="hrcA"
                     /locus_tag="PLU43_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOO28405.1"
                     /translation="MDLDERKKTILYTIIKTYLETGEPVGSRTISKYADLNLSSATIR
                     NEMSDLEDLGYIIQPHTSAGRIPSDKGYRFYVDQLMEDKEREVDEMKGILIEKADKME
                     RLLKQVAKSLAVNTNYATMISAPQYHRNKLKFIQLSRMEKDQIVAVIVVEGNIIKNRI
                     IQVEGGLDDETLLKLNILLNTNLNGLTIEEINLVIISKMKEQAGIHSNIISDVIDAVA
                     EAIKADEDLEIYTSGANNIFKYP"
     gene            complement(924..1295)
                     /locus_tag="PLU43_08065"
     CDS             complement(924..1295)
                     /locus_tag="PLU43_08065"
                     /inference="COORDINATES: protein motif:HMM:NF040112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HOO28406.1"
                     /translation="MSEAKKPEVVEAKKPKAVTKKVVQAAEKPVEPEKKEAAKAATTV
                     KKTTPVKKAPAKKAEVKENLYVQFGDWEIGQADVIAKVKEAYKAEGNKAAVKSMDVYV
                     KPEEKMVYYVVNGEITGSIEF"
     gene            complement(1465..>1912)
                     /locus_tag="PLU43_08070"
     CDS             complement(1465..>1912)
                     /locus_tag="PLU43_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOO28407.1"
                     /translation="KIPITNSLGESSIPLVGKLSKKGLRLNVTAILTLEQVKSTVDAF
                     APGTENIVSVFAGRIMDTGVDAMPIMKETARLCRTKPGTLSLWASCREIYNIVQADEC
                     GTDIITVTNALLGKMPNLGKDLTQFSLETVQMFARDGKSLGYTIRG"
BASE COUNT          458 a          486 c          311 g          657 t
ORIGIN      
        1 ggatatttaa aaatattatt agctccgctc gtatagatct ccagatcctc gtccgcctta
       61 atggcctctg ccaccgcatc gatcacatcg ctgataatat tgctgtgaat tcctgcctgt
      121 tccttcattt tagaaataat aacaagattg atttcttcta tcgtcagccc gttcaggttt
      181 gtattcagta aaatattcag ctttaacagc gtctcatcat ccagaccgcc ctcgacctga
      241 atgatccgat tctttataat attgccttcc actacgatga ctgctacgat ctgatccttt
      301 tccattctgg aaagctggat aaactttagt ttgttccggt gatactgcgg cgcggagatc
      361 atcgttgcat aattcgtatt gacagcaagc gatttagcga cctgtttgag caggcgttcc
      421 atcttatccg ccttttcgat aagaatccct ttcatctcat ctacttcccg ctctttatct
      481 tccatcagct gatccacata aaaacgatac cctttatcag aaggtatcct gccggctgaa
      541 gtatgtggct ggataatgta tccaagatct tccaggtcag acatttcatt acgaattgtt
      601 gctgaactca aattcagatc cgcgtatttt gaaatcgttc tggagccgac cggctcacct
      661 gtttccaaat acgttttgat gattgtatat aagatcgttt tttttctctc atccaggtcc
      721 actccttcac gtcctttctt gttttaccct gttagcactc aatgttgaag agtgctaaca
      781 tccatgtact taagatatca ctagtgtctt ttaatgtcaa caccatattt ctaaaataaa
      841 tataaactgc tgctaaacaa atataatcaa aattggagag acacaaacac cgtgtctctc
      901 caacttaaaa tttgtctggc tgattaaaat tcaatgctgc ctgtaatctc accattcact
      961 acataataaa ccattttctc ttccggcttt acatatacat ccatgctttt gaccgccgct
     1021 ttattgcctt ccgccttata agcttccttt acctttgcaa tcacatcagc ctgtccgatt
     1081 tcccaatcgc caaactgtac ataaaggttt tcttttactt ccgctttttt agccggtgct
     1141 tttttcactg gtgtcgtttt ctttactgtc gtcgccgcct ttgccgcttc ctttttttcc
     1201 ggttccacag gtttttcagc tgcctgtaca actttttttg taactgcttt tggtttcttt
     1261 gcttccacta cttccggttt ctttgcttct gacatagtgt tctcctttca gatttgcaag
     1321 tgtcttataa acttttccat atccccagat gtctttatag gaagatttta caaaatcttc
     1381 acttaagtgg taatataacg caaaattaca aaattgtcaa atttaccggc ggactttcgg
     1441 ctcatttaca gtgcttcccg gcattcagcc gcgtatcgtg tatccaagac tttttccgtc
     1501 ccgtgcaaac atttgtacag tttccagtga aaactgcgtc agatccttcc ctaaattagg
     1561 cattttccca agcaatgcat tcgttaccgt aataatatcc gttccgcatt cgtcagcctg
     1621 cacaatatta tagatctctc tgcagcttgc ccataaagac agtgttccag gctttgtcct
     1681 gcacagtctc gcagtttctt tcatgatcgg cattgcatca acaccggtat ccataattct
     1741 tcccgcaaat accgatacga tattttccgt gcccggtgca aatgcatcca ccgtactctt
     1801 tacctgttcc aatgtcagga tcgccgtcac atttaatctc aggccttttt ttgaaagttt
     1861 tccgaccagc ggaatactgg actcacccag cgagtttgtg atcgggatct tt
//
LOCUS       DAOSYE010000458         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50352_length_1912_cov_3.361874, whole genome shotgun sequence.
ACCESSION   DAOSYE010000458 DAOSYE010000000
VERSION     DAOSYE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50352_length_1912_cov_3.361874"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLU43_08075"
     CDS             complement(<1..738)
                     /locus_tag="PLU43_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28408.1"
                     /translation="MLVIYGFLTGGIAIFMLYLRYCIKRDKKLPWNRMLQNKIYPIMK
                     KLYSAKEAFENSRKFCEQKIKLCLFIVGIGCLSAFFYEIFLLNTQQLKEGCLIRRNAY
                     GEGNRQVWLWVVTEESGKHEKLKLLIPERAYTEEELADLFKQVPSDLINEILGDNSSL
                     NNVSKELDLVTSLEGYPFQISWSSDSPYLLSESGKIDENRLKEMENAEEGILISLTAE
                     LSYEEFTEFLDLYARVYPQPKSDEEQFL"
     gene            complement(764..1519)
                     /locus_tag="PLU43_08080"
     CDS             complement(764..1519)
                     /locus_tag="PLU43_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HOO28409.1"
                     /translation="MKHEGVIEYERYRLSFREGLLYLLEYLLMTSLTAYLFYDSVYAF
                     FVFLGGFVWFIRKKKRQLIQKRKKELSRQFQEAIEAAATALAAGFSVENAFLESQKDM
                     QKLYGTDSMIVRELQGLMQYLSVGKTLESFLLEFGERSGIEDIRDFGIVFSAAKRNGG
                     NFNQIIRKCVTIMHEKQETEYEIAVLLSGKNFEQKILSVIPFAIIGYLRCSTKGFLDI
                     LYHNPAGITVMSLCFAIYAAAYLLAEKIAAIEV"
     gene            complement(1509..>1912)
                     /locus_tag="PLU43_08085"
     CDS             complement(1509..>1912)
                     /locus_tag="PLU43_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO28410.1"
                     /translation="EVRGAEAIDMLQALNVGQDGSLSTIHANSAFDAISRLETMIMLH
                     TDIPLAALRRQIASGIDLIVHLGRLRDKSRRLIEIRELCGIENDGIKTAVLFQFAEEK
                     EEDGKIAGSIIKQEALCHVQKLKTAGINYEA"
BASE COUNT          503 a          490 c          320 g          599 t
ORIGIN      
        1 caaaaactgc tcttcatcac ttttaggctg cggataaact cttgcatata aatcaagaaa
       61 ttctgtaaac tcttcataac ttagttctgc cgtcaggctt ataagaatcc cttcctctgc
      121 gttttccatt tccttcagcc tgttttcatc gatttttccg ctttccgaaa gcaagtatgg
      181 actatcggag ctccagctga tctgaaacgg atatccttcc agcgaagtca cgagatccag
      241 ctcttttgat acattattca aagaactgtt atctccgagt atttcattga tcagatcgga
      301 tgggacctgt ttaaaaagat ccgcgagctc ctcctccgta tatgctcttt ccggtatcag
      361 aagctttaat ttttcatgct ttccggactc ttctgtcact acccacagcc agacctgccg
      421 gtttccttct ccatatgcat ttcttctgat caggcatcct tctttcagct gctgtgtgtt
      481 cagcagaaaa atttcataga aaaatgcgga aaggcagcca atacctacta tgaaaagaca
      541 aagttttatt ttttgttcac agaattttct gctgttttca aatgcctctt ttgcagaata
      601 cagttttttc atgatcggat atattttatt ctgcagcatc cgattccatg gcagcttttt
      661 gtcccttttt atacagtacc ttaaataaag cataaaaatt gcgataccgc ctgtcagaaa
      721 cccataaatg acgagcatgc ctccctcctt tccctgccga agcctaaacc tctattgccg
      781 caattttttc tgccagcaga taagccgccg cataaattgc aaagcacagg ctcatcactg
      841 tgattcctgc aggattgtga tacagaatat ccagaaaacc tttcgtactg catcttaaat
      901 aaccgattat tgcaaacggg atcacactca atattttttg ttcaaaattt tttccgctta
      961 aaagaactgc aatctcatat tcggtttcct gtttttcatg cataatagtt acacattttc
     1021 ttataatctg attaaaattc ccgccatttc gttttgccgc ggaaaaaacg atgccaaaat
     1081 ccctgatatc ttcgatcccg gagcgctctc caaattcaag cagaaaggat tccagtgtct
     1141 ttccaacgga caaatactgc atcagaccct gcagttctct cacgatcata ctgtctgttc
     1201 catataactt ctgcatatcc ttttgcgatt ctaaaaatgc attctcaacg gaaaatccgg
     1261 ctgcaagcgc tgttgcagca gcttcgatcg cttcctgaaa ctgccggctc agttcttttt
     1321 tcctcttttg aatcaactgt ctttttttct tcctgataaa ccagacaaac ccgccaagaa
     1381 aaacaaaaaa tgcgtaaaca gaatcataaa acaaatatgc agtcagactt gtcataagta
     1441 aatattccaa taaatacagc agtccttccc tgaacgaaag acgataccgc tcatactcga
     1501 ttactccttc atgcttcata gtttattcct gctgttttta atttttgtac atggcataat
     1561 gcctcctgct ttatgatact gccggctatt tttccgtctt cctctttttc ctccgcaaat
     1621 tggaacagca ctgctgtttt gatcccgtcg ttttctattc cgcacagctc cctgatctca
     1681 atcagacgcc tgctcttatc ccgcagtctg cccagatgga cgatcagatc aatccctgac
     1741 gcgatctgtc tgcgaagtgc ggcaagcgga atatccgtat gcagcatgat catggtttca
     1801 agacggctga ttgcatcaaa tgcgctgttc gcatgaatgg tagaaagcga accatcctgt
     1861 ccgacattga gtgcctgcag catatcgatc gcttcagcgc cgcgcacctc tc
//
LOCUS       DAOSYE010000459         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50362_length_1912_cov_2.942919, whole genome shotgun sequence.
ACCESSION   DAOSYE010000459 DAOSYE010000000
VERSION     DAOSYE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50362_length_1912_cov_2.942919"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLU43_08090"
     CDS             <1..1138
                     /locus_tag="PLU43_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOO28411.1"
                     /translation="TIAGGQSLVDGYNYNTLECVARESKVNQSDADGLYDIVWSNGLE
                     FNLYGDNLGGELKGLIEIRDGNNEEYFHDTVDSVSTGTNSDGVTVQTVSIDVTGGEDY
                     LTDLNKCTLPESGTITLGNKEFSYTEWEYDSATETYTFYLADGEDPASAYAGKEAAVG
                     TAVDYQGIPYYMEQMNEWIREFAEAMNEIELGAEDSYGDAAEVLFTGTNIVDNDDPYT
                     FADYYANIASGTTVTKSSDDSYYKLTAATFEVNDNMEEDVGKFGTTSDISQGQDAQDI
                     TEALLEVQTNKDMMSFRGCSSEEFLQTILADIALNASSANTFYGNYSNISSTITNQRL
                     SVSGVDNDEEALNLVKYQEAYNLASKMIQVMSEVYDRLILETGV"
     gene            1184..>1912
                     /locus_tag="PLU43_08095"
     CDS             1184..>1912
                     /locus_tag="PLU43_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28412.1"
                     /translation="MRITNSMMTNNSKSNINLNKLNEDKLNTMTASGQKITRPSDDPV
                     IAIRALRLNSSISELNQYYDKNIPDAEAWLEITETALTQTDSVLSSIKESLTTGASDD
                     NTAEDRQNILEDLSALRDEIYAAGNADYAGRTVFTGYRTGESLTFTEDTSSLYTITET
                     FSSSDVECMSYISGGIDVDKSDLSNANTEQDIESNDVYRIRLAYDTLTAAQVDQDGNA
                     LSGNILIDGTALEVPAGTPVQVQTV"
BASE COUNT          599 a          354 c          501 g          458 t
ORIGIN      
        1 aacgattgcc ggcggacagt cgcttgtgga tggttataat tataatacac tggaatgtgt
       61 ggcgagagaa tccaaggtca atcagtcgga tgcggatggt ctgtatgata tcgtgtggtc
      121 aaacggtctg gaatttaatt tgtacgggga caatcttggc ggtgaattaa aagggcttat
      181 tgaaatcaga gacggaaata atgaagaata ttttcatgat acggtcgaca gtgtaagtac
      241 cggaacaaac agcgacggag ttacggtaca gacggtttcc attgatgtta caggcggaga
      301 agattatctg acggatctga ataaatgtac actgccggaa agcggaacga tcactttagg
      361 aaataaggaa ttcagttata cggaatggga atacgattcc gcgacagaga cttatacgtt
      421 ttatctggct gacggagaag atcctgcaag tgcctatgca gggaaagaag cagcagtcgg
      481 aacagcggtc gactatcagg gaattcctta ttatatggaa cagatgaacg agtggatccg
      541 tgaatttgcg gaagcaatga atgaaataga actgggagcg gaagattctt atggagatgc
      601 tgccgaagtg ctgtttaccg gaacgaacat tgtagacaat gatgatccgt atacgtttgc
      661 cgattattat gcgaatatag cgagcggtac cactgttacg aaatcttccg atgacagtta
      721 ttataagctg acagccgcga cgtttgaagt caatgataat atggaggaag atgtcggcaa
      781 gtttggaacg acatcggata tttcacaggg tcaggatgcg caggatatta cggaggcact
      841 tttggaagta cagaccaata aagatatgat gagcttccgg ggctgctctt ccgaggaatt
      901 cctgcagacg atcttagcgg atatcgcatt gaatgcaagc agtgccaata cgttttacgg
      961 caattattcc aacatttctt cgacaattac caatcagcgg ttatcggtat ccggagtcga
     1021 taacgatgag gaagccctga atcttgtaaa ataccaggag gcttataatt tggcatcaaa
     1081 gatgattcag gtcatgtcgg aagtgtatga cagactgata ttggaaaccg gtgtataata
     1141 ttcaatggct gcagtatata tgaaatgaaa agggaggtta cttatgcgga ttacgaattc
     1201 aatgatgaca aacaactcaa aatcaaatat caatctcaat aaattgaacg aagataagct
     1261 gaatacaatg acagcatcag gacaaaaaat tacaagacct tctgatgatc cggtcattgc
     1321 gattcgggcg cttcgtctaa acagcagcat atcggaactc aatcagtact atgataagaa
     1381 cataccggat gccgaggcgt ggctggaaat tacagaaacg gcgctgacgc agacagattc
     1441 cgtattgtca agtatcaaag aaagtctgac taccggcgca agcgacgata atacggcgga
     1501 agacaggcag aatattcttg aagatctgag tgcgctgcgc gatgagatct atgcggcagg
     1561 caatgcggat tatgccggaa ggaccgtatt tacaggctat cgaacaggag aatctctgac
     1621 atttacggaa gatacgtcaa gtctgtatac cataacggag acattcagct cctccgatgt
     1681 ggaatgcatg tcatatattt ccggaggaat cgacgttgac aagtccgatc tgtccaatgc
     1741 gaatacagag caggatattg agagcaatga tgtatatagg atccggcttg cctatgatac
     1801 gctgactgct gcacaggtag atcaggatgg aaatgcactc agcggaaaca ttttgatcga
     1861 cggaaccgca ctggaagttc cggcaggcac gccggtacag gtacagaccg tg
//
LOCUS       DAOSYE010000460         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50428_length_1911_cov_2.743534, whole genome shotgun sequence.
ACCESSION   DAOSYE010000460 DAOSYE010000000
VERSION     DAOSYE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50428_length_1911_cov_2.743534"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLU43_08100"
     CDS             complement(<1..290)
                     /locus_tag="PLU43_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO28413.1"
                     /translation="MKYGYIKDADGNSYKLSKLIMGTVPFGSTMSKETSFTVMDNYVE
                     NGGNVIDTARVYSSWLPGGKDASETCVGEWLHERQNRNDIFLVTKGGHPPFE"
     gene            499..1836
                     /locus_tag="PLU43_08105"
     CDS             499..1836
                     /locus_tag="PLU43_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOO28414.1"
                     /translation="MEFRVQKEMSVTDKLQILSDAAKYDVACTSSGVDRRGDGSGMGN
                     AAAGGICHSFSADGRCISLLKILFTNECIYNCKYCINRADNDVVRTSFTPAEVCELTM
                     EFYRRNYIEGLFLSSGILHSPDYTMSLLYQTIYKLRKEYNFQGYIHVKAIPGSDEKLI
                     EKAGLLADRMSINLELPTADGLKELAPNKTRKTILRPMRQIQDGIVQNKNEIVLYRHA
                     PKFVPAGQSTQMVIGATGENDYQIVHVAESLYQKFALKRVFYSAFVNVNKDRDLPATL
                     EGPPLLREHRLYQADWLMRFYGFDAKELLSADRPNFNVMFDPKADWALRHLEYFPVEV
                     NKADYRMLLRVPGLGVKSAQRIVRARRIGPLGFDDLKRLGVVLKRALYFITCGGRMMY
                     HTKVEEDYIARNLLAVQERLPLGLSRDGMTYRQLSLFDDRTYGNRALRQEGEQ"
BASE COUNT          500 a          385 c          514 g          512 t
ORIGIN      
        1 ttttcaaaag gcgggtgccc cccttttgtg acaagaaaaa tatcgtttct gttctgcctt
       61 tcgtgcagcc attcgccgac acatgtttca ctggcatctt ttccgccggg cagccagctg
      121 ctgtatacac gtgccgtgtc gatgacgttc ccgccgtttt ccacatagtt gtccatgacc
      181 gtaaaagaag tttcttttga catggtggaa ccaaacggga ccgtacccat gatcagtttg
      241 gaaagcttat aagaatttcc gtccgcatct tttatatacc catacttcat aattgaccct
      301 ccgtttccgc aatcagatgt tacctggttc gtacagcgtt ttctctaata gttgttacca
      361 gtttattttg cgttttttgg gcgtcaatgt caagggttta aaatacaaac aaatgttctg
      421 aaaatatctt gaaaacagaa caaatgtttg gtatactatc cataggttca aaataaacgg
      481 cagaagcagg ggaatcttat ggaattcaga gtgcaaaaag aaatgtctgt tacagataag
      541 ctgcagatat tgtcggatgc ggcaaaatat gacgtagcct gtacaagtag cggcgtggac
      601 cgcagaggtg acggcagcgg gatgggaaat gcagcggccg gcggcatctg ccatagcttt
      661 tcggctgatg gaagatgtat ttccttattg aaaatattgt tcacgaatga gtgcatttat
      721 aactgcaagt actgtattaa tcgtgcggat aatgatgttg tgcgtacttc attcactccg
      781 gcggaagtct gtgaactgac gatggagttt taccggagga attacattga gggattgttt
      841 ttaagttccg gtattctgca cagtccggat tatacgatga gtctgcttta ccagacgatc
      901 tataagctgc ggaaggaata caattttcag gggtatattc atgtaaaggc gataccggga
      961 tcagatgaaa aactgataga gaaggcagga ctgcttgccg acagaatgag tatcaatctg
     1021 gagctgccga cggccgacgg tctgaaagag cttgcgccaa ataaaacgcg caagacgata
     1081 ttgcggccga tgcggcagat ccaggacgga attgtccaga ataaaaatga aatcgttctc
     1141 tatcggcatg caccgaaatt tgtgcctgcc ggtcaaagta cccagatggt cattggtgcg
     1201 acaggggaaa atgactatca gatcgttcat gttgcggaaa gtctctatca aaagtttgca
     1261 ttgaagcggg ttttttattc ggcatttgtc aatgtgaaca aagaccggga tctgccggcc
     1321 acactggaag gaccgccgct gcttcgggaa caccggctgt atcaggcaga ctggctgatg
     1381 cgcttttatg gatttgatgc aaaagagctg ctgagtgcgg accggccgaa tttcaatgtg
     1441 atgtttgatc cgaaggcaga ttgggcactc cgtcatctgg agtattttcc ggttgaagtg
     1501 aataaagctg actatcgaat gctgctgcgt gttccgggac tcggtgtgaa atcggcccag
     1561 cgcatcgtaa gggcaaggcg gatcggtccg cttggttttg atgatctgaa gcggctggga
     1621 gtcgtattga aacgtgcgct gtattttatt acatgcggcg gccgcatgat gtaccatacg
     1681 aaagtagaag aggactatat tgcaagaaat ctgcttgcag tgcaggaacg tctgcccctg
     1741 ggactttcga gggatggtat gacctatcgg cagctgagtc tgtttgacga ccggacttat
     1801 ggaaacaggg ctttacgtca ggagggggag caatgacagg aaagaggatt tatacttgtg
     1861 aagacagcct tgaggggatt tttactgcgg tttatgaggc atatgcggca g
//
LOCUS       DAOSYE010000461         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50519_length_1909_cov_2.937433, whole genome shotgun sequence.
ACCESSION   DAOSYE010000461 DAOSYE010000000
VERSION     DAOSYE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50519_length_1909_cov_2.937433"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1061
                     /locus_tag="PLU43_08110"
     CDS             156..1061
                     /locus_tag="PLU43_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO28415.1"
                     /translation="MTTISAGNLTGLVSDMNIISDSESLNQEKISIVEKYLSTIPEKL
                     LNLGIRILIVAILFIIGVQVIRFIRKIINRSLTKAAVETGSVNFIDACVKVVLYVFLI
                     VILAGNFGIDAASIVALMGSAALTIGLSFQGSLSNFAGGVLLLVSKTFKVGDYIIEGS
                     GKNEGTVTEIGLFYTKLKTIDNKIVIVPNGLLANTTIVNATGMEHRRMELVVGISYRA
                     DIEKAKDLIIELLHKESKVEHDKEMLVFVTALNSSSVDIGTRFWIRTEDYWPVRWALL
                     EKIKKTFDENNVEIPFNQLDVHMHP"
     gene            1174..>1909
                     /locus_tag="PLU43_08115"
     CDS             1174..>1909
                     /locus_tag="PLU43_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOO28416.1"
                     /translation="MSIFQAIIMGIIQGLTEFLPVSSSGHLAIMQNLLGIDTDTGLLF
                     DILLHIATLVAVFIVMYRDVIKLIVDFFLILKDFFINIGRAFKNLFSKKKTPYKVLAS
                     TSYRKFVIMLIISTIPTGILGYLMKDMVESVSTSLLIPGICLLATGFILLLSDFLEDG
                     YKKPKDAGYGDAFAIGSAQGVAVLPGISRSGTTIVACLLCGFDRKFAVKYSFIMSMPA
                     ILGAMILELKDLGGLQIELSEVVSYGV"
BASE COUNT          559 a          330 c          449 g          571 t
ORIGIN      
        1 gtacttttga acagcccacg gcggcagctg acgggaaaac agccggtaat gcaaaaagga
       61 agacgacagg gcataaaaag aatctgaaga aggaattgga aaagaagtat cagaaggaag
      121 aaagaggatt ataaaatagc atgcagaatt tgtttttgac aacgataagc gccggtaatc
      181 tgaccggact tgtttccgat atgaatatta tatcagacag tgaatcgctg aatcaggaaa
      241 aaatcagtat tgtagaaaaa tatttatcaa cgataccgga aaagctttta aatcttggta
      301 tccgtatatt gatcgttgcc atattattta taatcggtgt acaggtcatt cggtttatca
      361 gaaaaattat aaaccgttct cttacaaaag ctgctgttga aaccgggagc gttaatttta
      421 ttgatgcctg tgtaaaggtt gttctgtatg tattcctcat tgtaatactg gccggaaatt
      481 ttgggatcga tgccgcgtcg atcgtagccc tgatgggttc ggcggctctg acgatcggcc
      541 tgtcttttca gggaagcctt tcgaactttg caggcggcgt tctgctgctt gtttcaaaga
      601 catttaaagt tggagattat attattgagg gatccggaaa aaatgagggt accgtgactg
      661 aaatcggctt attttataca aagctgaaaa cgatcgacaa taagatcgta atcgttccaa
      721 acggtttgct tgccaatacg acaatcgtga atgcgaccgg tatggaacac cgccgtatgg
      781 aacttgtggt tgggatttct tatcgggccg atattgaaaa ggcaaaagat ctgattattg
      841 agcttttaca taaagagagt aaggtggaac atgataagga aatgcttgta ttcgttaccg
      901 cactgaattc cagttccgtg gatattggaa ccagattttg gataaggacg gaagactact
      961 ggccggtgcg ctgggcgctt ttggaaaaaa tcaaaaaaac ctttgacgaa aacaatgtgg
     1021 aaataccgtt taaccagctg gatgttcata tgcatccgta actgcaatgg ttgtaaatcc
     1081 gtatgattaa tggtataata ttcagcggaa ttatactgtt ttacatgaaa gcgggacacc
     1141 gtattatgta tcaaacaaat gaggaggagt tcaatgtcta tctttcaggc aattattatg
     1201 gggatcatac agggattgac ggaattttta ccggtaagca gttccggaca tcttgctatc
     1261 atgcaaaatt tactaggaat cgatactgat acaggactcc tgtttgatat tttgcttcat
     1321 attgcgacac tggtcgctgt ttttattgta atgtacagag atgttattaa gcttatcgtt
     1381 gatttctttc taattttaaa agactttttt atcaatatcg gacgtgcatt taaaaatctg
     1441 ttctcaaaga agaaaacacc atataaagtc ctggcaagta catcttatcg taaatttgtg
     1501 atcatgctga tcatttccac gataccaacc ggtattttag ggtatctgat gaaggatatg
     1561 gtagaaagcg tcagtacctc actcctgatc ccgggtatct gcctgcttgc taccggattt
     1621 attctgttgc tgtcagattt cctggaggat ggctataaaa agccaaaaga tgcaggctac
     1681 ggagatgcct ttgcaatcgg ttctgcacag ggagttgctg ttcttcccgg tatttcaaga
     1741 tcaggtacaa cgatcgtggc gtgtctgctc tgcggatttg acaggaagtt tgccgtgaaa
     1801 tattcattta ttatgtcaat gcctgcaatt cttggagcga tgatactgga actgaaggat
     1861 cttggaggtc tgcagattga gctttcggaa gtcgtttcct atggtgttg
//
LOCUS       DAOSYE010000462         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50705_length_1905_cov_2.351892, whole genome shotgun sequence.
ACCESSION   DAOSYE010000462 DAOSYE010000000
VERSION     DAOSYE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50705_length_1905_cov_2.351892"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..773)
                     /gene="pdxT"
                     /locus_tag="PLU43_08120"
     CDS             complement(213..773)
                     /gene="pdxT"
                     /locus_tag="PLU43_08120"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOO28417.1"
                     /translation="MRVAVLALQGAFMEHEQMLWKIGTDTVELRKKEDLDRPFHALIL
                     PGGESTVQGKLLRELGMFDGLQERIREGLPVLATCAGLILLAEEISNDKNVYFGTLPV
                     TVKRNAYGRQLSSFHTEEMFEGLGIQPMEFIRAPYIEHAGKDVEILSGSGDRITAVRY
                     KNQLAMSFHPELCENTDIHEAFIKNI"
     gene            complement(775..1650)
                     /gene="pdxS"
                     /locus_tag="PLU43_08125"
     CDS             complement(775..1650)
                     /gene="pdxS"
                     /locus_tag="PLU43_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOO28418.1"
                     /translation="MDEKRAALNRELAKMLKGGVIMDVTTKEQAKIAEEAGACAVMAL
                     ERIPADIRAAGGVSRMSDPKLIREIQQAVSIPVMAKCRIGHFVEAQLLEAIEIDYIDE
                     SEVLSPADDSCHIDKTTFNVPFVCGAKDLGEALRRIDEGASMIRTKGEPGTGDIIQAV
                     RHMRLMNAAIAKIAAMREDELFNEAKELRVSCELIRRVHKERRLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGNPGKRAAAIVKAVTNYQDAKMIAALSEDLGEAMVG
                     INEEEIELLMAERGN"
BASE COUNT          444 a          551 c          349 g          561 t
ORIGIN      
        1 cctgattcct ccgtctgttc ggccgtaaaa ccgatccctc cggctccttt tgcaatagaa
       61 agaaacgaac cgagctcctt cataataaaa cgcaaatgta cgccgcactt tctgctgtcc
      121 actctcatcc gtgtttcctc catcatccca ggctgttaaa tcattctatc aaaaatgacc
      181 gtatttttca acatgggaga cggaccgaaa tcctatatat ttttaataaa ggcttcgtga
      241 atatccgtat tttcacaaag ctccggatga aaggacattg caagctgatt tttgtaacgc
      301 accgcagtga tcctgtctcc tgatcctgag agtatttcca catcttttcc ggcatgctcg
      361 atatagggag ctcttataaa ttccatcggc tgtatcccaa gtccttcaaa catttcttcc
      421 gtatggaagc tggaaagctg ccttccgtat gcatttcgtt ttactgtgac cggcagcgtt
      481 ccaaaataaa catttttgtc gttggatatc tcttccgcca gaaggatcaa tcccgcacag
      541 gttgcaagaa caggaagccc ttctctgatc ctttcctgca gtccgtcaaa catcccaagc
      601 tcccgcaaaa gcttcccctg cacggtgctt tcgccgcccg gcagtataag tgcatggaac
      661 ggtctgtcca gatcttcttt ttttcgcagt tcaaccgtat cggtcccgat tttccaaagc
      721 atctgctcat gctccataaa ggctccctgc agtgccagta ccgctactct catatcagtt
      781 tcccctttcc gccatcagca gttcgatctc ctcttcattg atgcctacca tagcctcgcc
      841 aaggtcttca gaaagtgccg cgatcatttt ggcatcctgg taatttgtaa ccgcttttac
      901 gatcgccgcc gccctttttc cgggattgcc ggatttgaaa ataccggagc cgacaaaaac
      961 cccttctgcc ccaagctgca tcatcagggc agcatctgcc ggtgtcgcca ctcctcctgc
     1021 cgcaaaattt acgaccggaa gccgcctttc cttatgtacg cgccttatga gctcacagga
     1081 tacccgcagc tccttcgctt cattaaacag ctcatcttcc cgcattgccg ctattttagc
     1141 gatcgccgca ttcatcagac gcatatgacg cactgcctgg ataatgtctc ctgtccccgg
     1201 ttctcccttt gtccgtatca tcgatgcccc ctcatcgatc ctgcgcagcg cttcaccaag
     1261 gtcctttgct ccgcacacaa acggtacgtt aaaggtcgtc ttatcaatgt gacagctgtc
     1321 gtcggccggc gataagacct cgctttcatc gatataatca atttcgatcg cttccagaag
     1381 ctgtgcttcc acaaaatgac cgatcctgca ttttgccatg accggtattg acaccgcctg
     1441 ctggatctcc ctgatcagct ttggatcgct cattcttgag acaccgcccg ccgcgcggat
     1501 atccgccggt atccgttcca gtgccatgac cgcacaggct cctgcctcct ccgcaatttt
     1561 agcctgttct ttcgtcgtca catccataat gactccgcct ttaagcattt tcgcaagttc
     1621 tctgttcaat gcagctcttt tttcatccat tctcttgatc ctccgtttca taaaatgatg
     1681 ctgtttgatc ctttactttt tacacaaaat ttattataat gaaatctgaa cttattaaaa
     1741 tattcatttt taatataatc aaaaaggtca gttgcggaga tatataatat tatgttaact
     1801 tattcttttg aaaatctagg gtcagaaagc ctgtatcacc atttatataa atgcataaaa
     1861 aatgacattt tagcaggaat tctcctgcct ggcacgcatc tgcca
//
LOCUS       DAOSYE010000463         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50723_length_1904_cov_3.542996, whole genome shotgun sequence.
ACCESSION   DAOSYE010000463 DAOSYE010000000
VERSION     DAOSYE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50723_length_1904_cov_3.542996"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLU43_08130"
     CDS             <1..685
                     /locus_tag="PLU43_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO28419.1"
                     /translation="RIGSFYRIIKSEKPQLIHVSMSESLITFGVLRCLGFFKRIPFVY
                     TDRGLCYGYRRHSKTCIMKTMRYAAGMICTTAYNKGLWTKEQMPCEITVIPNTISKAF
                     DVYTDGKRMEERRAHGIADGEFVVGFAGRISEEKDWPFVEHLTAALRESGMRFKVALV
                     LSVYEEQDAQIVKEIKDGIVKSIGRENLIYMQDLSQREIADYYYMVDVFVMSSVFESF
                     GKAAVEAMS"
     gene            788..928
                     /locus_tag="PLU43_08135"
     CDS             788..928
                     /locus_tag="PLU43_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28420.1"
                     /translation="MNRLCTDQEELEKDREFFYTRYLDHYTKEVHIGRHMEFYHAVLK
                     KS"
     gene            1029..>1904
                     /locus_tag="PLU43_08140"
     CDS             1029..>1904
                     /locus_tag="PLU43_08140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO28421.1"
                     /translation="MNVSANRLDRGFYQYQEEFEQKALEVLRSGWYVLGREVASFEEE
                     FAAYTGTKYCVGLGNGLDALWLAFRVLGVGSGDEVIVQGNTYIASVMGITINGATPVF
                     VEPDEYYLIDADRIEEKITEKTKAVLAVHLYGQASKMDQLIALCKKHRLYLIEDCAQS
                     HGACFNKQMTGSFGDIGCFSFYPSKNIGAFGDGGAIVTNNEKIAADMRMYRNYGSEKR
                     YYNKVIGANSRLDEIQAGLLRVRLKHAEELTGEKLEIAKRYRTGLKNSLIELPKVREK
                     ADHVYHQFVIRCRRRD"
BASE COUNT          545 a          338 c          542 g          479 t
ORIGIN      
        1 caggatcggt tccttttacc ggatcatcaa atcggaaaaa ccgcagctga tccatgtatc
       61 gatgtcggaa tctttaatta cgttcggggt cttaagatgc cttggatttt ttaagcggat
      121 accgttcgtt tatacggaca ggggcctttg ctacggctac cgcagacatt ccaagacctg
      181 tattatgaaa acgatgagat atgcggccgg aatgatttgt acgacggcct ataataaagg
      241 actttggacg aaggagcaga tgccgtgtga gatcacggtc attccgaata cgatcagcaa
      301 ggcgtttgat gtatacacgg acgggaagcg catggaagag cgcagagcgc atgggatcgc
      361 agacggagag tttgttgttg gatttgcggg acgcatttca gaggaaaagg actggccgtt
      421 tgtggagcat ttgacggcag ctttgcggga gagcggaatg cggtttaagg tagcccttgt
      481 gctttccgta tatgaggagc aggatgcaca gatcgtgaag gaaatcaaag acggcattgt
      541 gaagagcatt ggccgggaaa atttgattta tatgcaggat ctgtcccaga gggaaatcgc
      601 cgattattat tatatggtgg atgtgtttgt gatgtcctcg gtatttgaat cgttcggcaa
      661 ggcggcagta gaagcgatga gctgaaaatg cgcggtcgta tctacgagcg tcggcggtct
      721 gactgaagtt gtcggtaagg aggaaaacct ctatacaaaa aatgatctgc ataaatttac
      781 ggaaagaatg aaccgcctgt gcacggatca ggaggaactg gaaaaagaca gggagttttt
      841 ttatacgcgc tatctcgatc attatacaaa agaagttcat atcggcaggc acatggaatt
      901 ttatcatgcg gttcttaaaa agtcctgact gcatggtatg cccgttgctt gtgtcttttt
      961 tgtaataatg atataatcga cggtaatgac agaattcgta cgggtaaacc gtatcaggag
     1021 gaaagaggat gaatgtatct gcaaacaggt tagacagagg attctatcaa tatcaggaag
     1081 aatttgaaca gaaagcgttg gaggtgctgc gttccggctg gtacgtactt ggcagggagg
     1141 tcgcttcttt tgaagaggaa tttgccgctt atacagggac aaaatactgt gtcggcttag
     1201 gcaacggact ggatgcactg tggcttgctt tccgtgtact cggtgtcgga agcggcgatg
     1261 aagtcattgt acagggaaac acttatattg caagtgtgat gggaattaca ataaacggcg
     1321 caacaccggt atttgttgag ccggatgaat actatctgat agatgccgac aggatcgagg
     1381 aaaaaattac ggaaaagaca aaggcggtac ttgccgtgca tttatatggg caggcttcta
     1441 aaatggatca gctgatcgct ttatgtaaaa aacatcggtt atatctgata gaagactgtg
     1501 cgcagtcgca cggggcctgt tttaataagc agatgacggg cagttttggg gatatcggct
     1561 gtttttcatt ctatccttca aaaaatatcg gcgcatttgg ggacggcggt gcaattgtga
     1621 ctaataatga gaaaatagcg gccgatatgc ggatgtaccg caattatgga agtgaaaaac
     1681 gttactataa caaagtgatc ggtgccaatt ccagactgga tgagatacag gccggactgc
     1741 ttcgtgtcag actgaagcat gcggaggagc ttaccggaga aaagctggaa attgcaaaaa
     1801 gatacaggac gggtcttaaa aattcgctga tcgaactgcc gaaggtaaga gaaaaagcag
     1861 accacgtata tcatcagttt gtgatccgct gcaggcggag ggat
//
LOCUS       DAOSYE010000464         1900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_50920_length_1900_cov_3.873171, whole genome shotgun sequence.
ACCESSION   DAOSYE010000464 DAOSYE010000000
VERSION     DAOSYE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50920_length_1900_cov_3.873171"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PLU43_08145"
     CDS             <1..1533
                     /locus_tag="PLU43_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28422.1"
                     /translation="LAVSGIVALAQWVMNILNNRTAYCVVRDIRNEAFKKIEILPLRY
                     LDAHSQGEIVSRVIADVDQFADGLLMGFTQLFTGVVTILGTLGFMLFLNVKITLVVVI
                     ITPLSLFAARFIAKKTFRMFREQSEIRGEQTAFVDEMIGNEKVVKAFSQEEKMLAKFD
                     EINQKLQKASLYAIFFSSLTNPVTRFVNGVVYAGVALTGAMAVLSGKMTVGVLTCFLS
                     YANQYTKPFNEISGVVTELQNAIACAERIFELIEEEPCKFEAENAEVLADVGGRVVCE
                     HVDFSYTADSPLLSDMNISVQPGERVAIVGPTGCGKTTLINLLMRFYDADSGSIRVDG
                     TDIKNVTLHSLRTSYGMVLQDTWLRNGTVRENIIMGKLNAAEEEIIAAAKEAHAHSFI
                     RRLPDGYDTVIGEDGGMLSQGQKQLLCITRVMLCLPPMLILDEATSSIDTRTEVKIQS
                     AFQKLMKGKTSFIVAHRLSTIRNADMILVMKNGRIIEQGTHEALLEQNGFYNELYNSQ
                     YS"
     gene            1654..>1900
                     /locus_tag="PLU43_08150"
     CDS             1654..>1900
                     /locus_tag="PLU43_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO28423.1"
                     /translation="MKVNYVQDMTKGNELSLLAGFSAPMLIGNIFQQFYNMVDSIIVG
                     NYVGADALAAVGATGSLNFLFFSLCNGLAMGIGILISQ"
BASE COUNT          519 a          348 c          489 g          544 t
ORIGIN      
        1 ctggctgtat ccggaatcgt tgcactggca caatgggtga tgaatatttt aaataacaga
       61 acagcttatt gtgtcgtgcg ggatatacga aatgaagcgt ttaaaaagat tgaaatactg
      121 ccgcttcgtt atctggatgc gcattcccag ggagagatcg tcagccgtgt gattgcggat
      181 gttgatcagt ttgcggatgg tctgctgatg ggattcacac aactgtttac aggtgtcgtg
      241 acgattcttg gaacacttgg ttttatgctt ttcctgaatg tgaaaataac gctcgttgtt
      301 gtgatcataa cgccgctgtc tttgtttgcg gctagattta ttgcaaaaaa aacgtttcgg
      361 atgtttcgcg aacaatctga aatccgcgga gaacaaacgg catttgttga tgagatgatc
      421 ggcaatgaga aggtagtaaa ggctttttca caggaggaga agatgcttgc aaaatttgat
      481 gaaattaatc aaaagctgca gaaagcttcc ctttatgcga tctttttttc ctctcttaca
      541 aatccggtta cacggtttgt taacggtgtt gtttatgccg gtgttgcact gacaggtgca
      601 atggcggtgc tgagcggaaa gatgacagtt ggtgttctga cctgtttttt gagttatgca
      661 aatcaatata caaagccatt caatgaaatt tccggtgttg taacagagct gcagaatgca
      721 attgcatgtg cagaacgtat ttttgagctg attgaagaag agccctgtaa atttgaagcg
      781 gaaaatgcgg aggtgcttgc agatgtagga ggcagagtag tctgtgagca tgtggatttt
      841 tcctatactg cggacagtcc cctgctttcg gacatgaata tttcggtaca accgggtgaa
      901 cgggtggcga tcgtcggacc gacaggctgc ggcaagacaa cgctgatcaa cctgcttatg
      961 cgtttttatg atgcagacag cggcagtatc cgtgttgacg gtacagatat aaaaaatgtg
     1021 accttacaca gccttcgtac ttcctatggg atggtgctgc aggatacgtg gcttagaaac
     1081 ggaacggttc gcgaaaatat tattatggga aaactaaacg cagcagagga ggagatcata
     1141 gcggctgcaa aggaagcgca tgcgcattcg ttcatcagaa ggctgccgga cggatacgat
     1201 acggtcatcg gagaagacgg cggtatgctt tctcagggac aaaagcagct tttatgtatt
     1261 acgcgagtga tgctgtgcct gcccccgatg ctgatattgg atgaagcgac ttcttcgatc
     1321 gacacccgca ccgaagtgaa aatacaaagc gccttccaga aattgatgaa aggaaagacc
     1381 agttttattg ttgcgcatcg attatctacg atcaggaatg cagatatgat tcttgttatg
     1441 aaaaacggca gaattattga gcagggcaca catgaagcgc ttcttgaaca gaatggattt
     1501 tataatgaat tgtataacag tcagtattcc tgacaacatg gcttattgta gttggaatag
     1561 atttatacaa tttttgaata acattcctat agcacttttg tctgcacgat gctatagtgc
     1621 accttagatg ggaaaataaa tggggtgctt gctatgaaag taaattatgt gcaggatatg
     1681 acgaaaggga atgaactttc cctgctggcc ggattttcgg cgccaatgct gatcggaaat
     1741 atatttcaac aattttataa tatggtggat tccatcattg tcggcaatta tgtcggagcg
     1801 gatgcacttg ccgcagttgg cgcgaccggc tctcttaatt ttttgttttt ttctttatgc
     1861 aacggtctgg ccatgggaat cgggatactg atctcccaat
//
LOCUS       DAOSYE010000465         1896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51106_length_1896_cov_4.016295, whole genome shotgun sequence.
ACCESSION   DAOSYE010000465 DAOSYE010000000
VERSION     DAOSYE010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51106_length_1896_cov_4.016295"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1584)
                     /gene="rlmD"
                     /locus_tag="PLU43_08155"
     CDS             complement(157..1584)
                     /gene="rlmD"
                     /locus_tag="PLU43_08155"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOO28424.1"
                     /translation="MKKEQILEGTVQTIKFPNKGIVSISEDSSEKRQCIVKHVLPGQR
                     IRFAVQKFRSGSCEGRLLEVLERSPAEADSACRHFPACGGCAYQNLPYEEQLKLKEKQ
                     VRELLESALHDVSDVFEGIKPSPVQTGYRNKMEFSFGDSSKGGPLELGMHKRGSFYDI
                     VTVTDCQIIDADYQKILTETLSCFREAGLSFYHKLTHTGYLRHLLVRKAARTGEILVD
                     LITATQTSFDNGHAEQALLNDWQTRLLSLPLSGRLTGILHTRNDSVADMIRNDGTDIL
                     YGQDYFYEELLGLKFKISPFSFFQTNSLSAEVLYETARQYVSLAQSGVDLQGAVVYDL
                     YSGTGTIAQLLAPAAGKVIGVEIVEEAVEAARENAVLNGLHNCEFIAGDVLQVLDEIE
                     KKPDFIVLDPPRDGVHPKALKKIIRYGVEHIVYISCKPTSLVRDLETFLGNGYRVVRA
                     CAIDQFPGTVHVESVVLLTRINTEK"
     gene            complement(1656..>1896)
                     /locus_tag="PLU43_08160"
     CDS             complement(1656..>1896)
                     /locus_tag="PLU43_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOO28425.1"
                     /translation="FPEDALCYEAEDQYLFGPKLLVAPVMEPHVVTRSVYLPAGCQWK
                     EIATGKIYDGGVLIDAKAPIDIIPVFEIVKDEMLL"
BASE COUNT          468 a          505 c          428 g          495 t
ORIGIN      
        1 ggcggattct ggactttcac cagttagaaa cgtgcgccgc caggcgcact aaaaataagg
       61 ggctgaacag ttcaatgtgt tcaacccctt attttttaat ttgttcaatg gtggcaaatg
      121 ggtggcaaag ccaccgaaaa caccacttga aatcacttac ttttcagtgt ttatgcgtgt
      181 tagaagcact actgattcaa catgcacggt tcctggaaac tggtcaatcg cacaggcgcg
      241 gacgacccgg tagccatttc caagaaaggt ttccagatcg cggacaaggc tcgtcggctt
      301 gcaggaaatg tagacaatat gctccacacc gtaacggatg atttttttaa gtgctttcgg
      361 atgaacgcca tcgcggggcg ggtccagcac aataaaatcc ggtttctttt cgatttcatc
      421 gagtacctgc agcacatcgc cggcaatgaa ttcgcaatta tgtaaaccgt ttaagacagc
      481 attttctctt gccgcttcta cggcttcttc cacgatctca acaccgatta ccttgccggc
      541 tgccggtgca agaagctgcg cgatcgtgcc ggttccgctg tatagatcat agacaacggc
      601 gccctgcaga tctaccccgg actgtgcaag cgacacatac tgcctggctg tttcatacag
      661 cacttcggca ctgagactgt tcgtctgaaa aaaagaaaac ggagaaatct taaatttcaa
      721 tccaagcagc tcctcataaa aataatcctg tccgtataaa atatccgttc catcgtttcg
      781 gatcatatct gcgacagaat cattccgtgt atgcaggatc ccagtgagcc tgccggaaag
      841 cggcagagat aagagccggg tctgccagtc gtttaacaaa gcctgctctg cgtggccgtt
      901 atcaaaggat gtttgtgtcg cagtgatcag gtccacaaga atttctccgg tcctggctgc
      961 ctttctgaca agcaggtgac gcagatagcc ggtatgcgtc agcttatgat aaaaggaaag
     1021 tcctgcttcc cgaaaacagg acagtgtctc tgtcaggatc ttttgataat ctgcatcaat
     1081 gatctgacaa tccgtaactg ttacgatgtc ataaaagctg ccgcgcttgt gcatgccaag
     1141 ctccagggga ccgcccttgc tgctgtctcc aaaagaaaat tccatcttat tgcggtaacc
     1201 agtctgtacc ggactcggct tgattccctc aaagacatcg gaaacatcgt gcaatgcgga
     1261 ttccagtagc tccctgacct gcttttcttt cagcttcagc tgttcctcat agggcaggtt
     1321 ctgataggca cagccgccgc acgcaggaaa atgccggcag gcactgtccg cttccgccgg
     1381 tgatctttcc agtacttcca acagcctgcc ttcgcagctg ccgctcctga acttctgcac
     1441 ggcaaaacgg atcctctggc ccggcaggac atgtttgacg atgcactgcc ttttttcaga
     1501 agaatcttct gatatcgata cgattccctt attcggaaat ttgattgttt gtactgttcc
     1561 ttccagtatc tgctcttttt tcatatacac tccatttatt ctgatttatg ttaatctgct
     1621 gtcgctgtta taacaatcta acagccatga aaacattaca gcagcatctc gtctttcacg
     1681 atctcaaata ccggaatgat gtcgatcggc gcctttgcat cgatcaggac accgccgtca
     1741 tagatctttc ctgtcgcaat ctccttccac tgacagccgg ccggcagata gacgctgcgc
     1801 gtaacgacat gcggctccat gaccggcgct acaagaagct ttggtccgaa cagatactga
     1861 tcctcagcct catagcagag ggcatcttct gggaaa
//
LOCUS       DAOSYE010000466         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51213_length_1894_cov_2.433931, whole genome shotgun sequence.
ACCESSION   DAOSYE010000466 DAOSYE010000000
VERSION     DAOSYE010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51213_length_1894_cov_2.433931"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1894
                     /locus_tag="PLU43_08165"
     CDS             171..>1894
                     /locus_tag="PLU43_08165"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOO28426.1"
                     /translation="MAIQIAAVLICLLCVVLLVSHEDTRLSKLMLVIVICGFVQNAGF
                     LMELQSTSLAEVMSAVRVEYIGSSFIASLLTVFVFGYCKARLPVPIRYLMFLFSAAVF
                     VLVWTYPHNLLYYTGAEFVDTGYFPHVVLDKGPVYVIYAGMIYTELICCGFLSLLTAL
                     RAEEKRMRMNCFLLFLSCMVPFAAHLANFAEALEGYDSTPPGVAFGIAVFGIAVARQH
                     IFDVVETAHESILMNLDDAIIIVDQAYGFKEANKKAKELFPVLHDVKYGARVQDMDFN
                     RIFNKDGVRELVIGERFYDIHINQVLYNNKTAGYTAILFDVTENKYQLEKMRELKEQA
                     DQANRAKSAFLANVSHEIRTPLNAVLGINEVILRDYEEPQLLEYSRNIHNAAKALLQL
                     INDILDFSKIEAGKFELIAARYHTGNLFSELISAYGFRIKEKGLRFSANIAGNIPKYL
                     IGDAIRIRQVLFNMLSNAVKYTDEGEITMHAVFERTGREMGNLILSVEDTGIGIRKED
                     MGKLFECFVRLNERNDRTTEGTGLGLNITKQLIDLMNGQLKVTSEYGKGSVFTAVIPQ
                     LVSEGTEE"
BASE COUNT          518 a          346 c          500 g          530 t
ORIGIN      
        1 aggtcgcagg agactgccgg cgacggcagt accagacaga tgattgtccg gaacacggct
       61 gtgttccgac agaacccggc ttatcgttcc gctcatattt tataaagttg cctatattga
      121 aattgcaaga tgcgatagcg gaaaggagtg tcacaatgta tcagatcctg ttggctatac
      181 agattgcagc agttttgatc tgcctgttat gtgtcgtcct tctggtatct catgaggata
      241 caagactttc aaagctgatg ctggtgatcg tcatatgcgg cttcgttcag aatgccggtt
      301 tcttgatgga attacaatcc acatctcttg cagaggtcat gtccgcagtc cgggtggaat
      361 atatcggaag ctcttttatt gcgtcgcttc tgacggtttt tgtatttggg tactgcaaag
      421 ccaggctgcc ggtaccgatt cgatatctga tgtttttgtt cagtgcggct gtctttgttc
      481 ttgtctggac atatccgcat aatctgcttt attataccgg tgctgagttt gtcgatacgg
      541 gttattttcc ccatgttgta ttggacaagg gaccggttta tgttatatat gccggcatga
      601 tctatacgga actgatctgc tgcggatttc tatcgctgct gacggctttg cgggctgaag
      661 agaaaaggat gcgcatgaat tgtttcctgc tgtttttaag ctgtatggtt ccatttgcag
      721 cgcatctggc aaatttcgcg gaagcactgg agggttatga ttcgacgccg ccgggtgtgg
      781 catttggaat tgcggtattt gggatagctg ttgcacgcca gcatattttt gatgtagtgg
      841 agaccgcaca tgaaagtatt ttgatgaact tggatgatgc gattattatt gtggatcagg
      901 cttatgggtt taaagaagca aataagaaag caaaagagtt gtttccggtg cttcatgatg
      961 tgaaatacgg cgccagggtc caggatatgg attttaacag aattttcaac aaagacggtg
     1021 tcagggagct tgtgatcgga gaacgttttt atgatatcca tatcaatcag gtcctttata
     1081 ataacaaaac agccggttat actgctattt tgtttgatgt aacggaaaat aaataccagc
     1141 tggagaaaat gcgggaatta aaagaacagg cggatcaggc aaacagggca aagtccgcat
     1201 ttctggcaaa cgtatcccat gagatcagaa ctccgctcaa tgccgtactt gggatcaatg
     1261 aagtgatttt aagggattat gaggagccgc agctgcttga atattcacgg aacattcata
     1321 atgctgcaaa ggcgcttctt cagctgatca atgatatttt ggatttttca aaaatagagg
     1381 ctggaaagtt tgaattgatt gcggcaagat atcataccgg caatcttttc agtgaactga
     1441 tctctgccta tggatttagg ataaaggaga aggggctgcg gttttcagcc aatatagcgg
     1501 gcaatattcc gaaatatctg atcggagatg ccataaggat caggcaggtg cttttcaaca
     1561 tgctttccaa tgccgtaaaa tatacggatg aaggagaaat taccatgcat gcggtatttg
     1621 aaagaacagg cagggaaatg ggaaacctga ttctttcagt ggaagatacg ggaatcggga
     1681 tccgtaaaga agacatggga aaactgtttg aatgctttgt aagactgaat gagagaaatg
     1741 accgcacaac ggaagggacc ggacttggac tcaacattac aaagcagctg atcgatctga
     1801 tgaacggaca gctgaaagtg acgagcgaat acgggaaagg atccgtattt acagcagtga
     1861 taccgcagct tgtcagtgaa gggaccgagg aaga
//
LOCUS       DAOSYE010000467         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51502_length_1888_cov_3.721768, whole genome shotgun sequence.
ACCESSION   DAOSYE010000467 DAOSYE010000000
VERSION     DAOSYE010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51502_length_1888_cov_3.721768"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..1653)
                     /locus_tag="PLU43_08170"
     CDS             complement(319..1653)
                     /locus_tag="PLU43_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28427.1"
                     /translation="MNIERKGKGVMKKKIISAVLSAAMIAGLLSGCGSDAKAVSSAQE
                     PESTETSASSEEPVEINMFIASPEYADAINELIAAYKEVKPNVTINYETTQNDYPTLL
                     KTKLNAGECPDIFSSTSGKEIVTYLDYSYDLSGQPLADAMSDSVADVMKSGDGLYGLS
                     IKGNYFGLLYNKAIFEECGISEFPQTLDDLEAACEKIQAKGYQPFTTGYAEWWVFKHT
                     FQSFLDAAGKDDVEGLVNKFAAGEAHFSDYPELYDDFFRYLDMTVKYGDAKPLETDLS
                     AEDAAFGSGQAAIMNGQGAWVEADVLAIDQNIQIGFDGYPVGSDASLCKVIGGSDQAL
                     RVYKDSKVLDDVLDFVNWWYTSDYGKAWFNEVAGVIPPIDDAAAPDFAVIKQGNELAS
                     AEGTNILSIVYSTDSFHQAFGEVLQSYIAGTIDKDTACAQIEEKWTELEGGN"
BASE COUNT          490 a          501 c          346 g          551 t
ORIGIN      
        1 cctgctttca atttttttac acggtgcaac gatccgcatg ccgtttttta tttcttcaac
       61 gtaatatgca tccgaagcat aaaatgcggt ggcgcgttcg ctttttaacc agtaaccttc
      121 tgaaaatttc attgtctgat cctctctttc cccatatcct tttaattata tttgaatgat
      181 ctaaaatttt taccattagt gtaattataa tgaattttat cattttcgta aatgttttgc
      241 agagctttct tttgtattat agcgacaaaa acagggaccg caattttgcg atccctgttc
      301 gtatatacgg ctgcctgttc aattgccgcc ttcaagctca gtccattttt cctcgatctg
      361 tgcacatgcc gtatccttgt cgatcgtccc tgcaatataa gactgcagaa cttcgccgaa
      421 tgcctgatgg aaactgtcgg tcgaataaac aatggataat atatttgtac cttctgccga
      481 tgcaagctca ttcccctgct tgatcactgc aaagtcagga gctgccgcgt catcgatcgg
      541 aggaatcacg ccggccactt cattgaacca ggcttttcca taatcggacg tataccacca
      601 attgacaaaa tcaagcacat catccagcac cttcgaatcc ttgtaaacac gaagagcctg
      661 atcggaacca ccaataacct tgcacaaaga ggcatcactg ccgaccggat acccgtcaaa
      721 accgatctgg atattctgat cgatcgcaag cacatctgct tccacccatg ctccctggcc
      781 attcatgatc gctgcctgcc cggaaccaaa cgctgcgtcc tccgcgctca gatcagtttc
      841 aagaggcttt gcatccccat acttgacagt catgtccaga tacctgaaaa agtcgtcata
      901 aagctcagga taatcactga aatgggcttc tcccgctgcg aatttattaa caagaccttc
      961 cacatcgtcc tttcctgccg catccaaaaa tgactggaat gtatgcttaa acacccacca
     1021 ttcggcatag cctgttgtaa acggctgata tccctttgcc tgaatctttt cacaggctgc
     1081 ttccagatca tccagtgtct gcggaaattc actgattccg cattcttcaa atatcgcctt
     1141 attatagagc agaccaaaat aatttccttt tatgctgagg ccataaagcc cgtctccgga
     1201 tttcatcacg tcggcgaccg aatcactcat tgcatccgca agcggctgtc cggacaaatc
     1261 atatgaatag tccagatacg taacgatctc ctttcctgag gtcgatgaaa aaatatcggg
     1321 acactctcca gcattcagct ttgttttcaa aagtgtcgga taatcattct gtgttgtctc
     1381 gtaattgatc gttacattgg gttttacttc tttataagca gcgatcagct cattgattgc
     1441 atccgcatac tccggacttg caataaacat attgatttcg accggctcct cagaggaagc
     1501 cgatgtttcc gtactttccg gctcttgtgc cgatgaaact gcttttgcat ccgaaccgca
     1561 tccggaaagc aggccggcaa tcattgcggc cgaaagaact gcacttatta ttttcttttt
     1621 cataacaccc tttcccttcc tttcgatatt cattgtttta gtttcctgta tatgatccgc
     1681 acccggccgg aatacggaaa atatcataat ttctcagcct ttgacagcgc cggctacgac
     1741 gccctccaca atatatttct gcagcactat aaaaaacacg atcgagggca ctaccgcaag
     1801 caccatggct gtcattgcgt aatgccattg tgtattaaac tgaccgacaa aattatacgc
     1861 tgcaagtaca agcgttttcg tattcggc
//
LOCUS       DAOSYE010000468         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51518_length_1888_cov_2.307692, whole genome shotgun sequence.
ACCESSION   DAOSYE010000468 DAOSYE010000000
VERSION     DAOSYE010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51518_length_1888_cov_2.307692"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1888)
                     /locus_tag="PLU43_08175"
     CDS             complement(<1..>1888)
                     /locus_tag="PLU43_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOO28428.1"
                     /translation="ALKNITERYDGYGSSIRSVMERKDSIKGLIGVVADIISVEKKYE
                     TAIETALGSNIQNIVTADEQVAREMIRYLKENKFGRATFLPLTSVRARGEFGRPEIRE
                     EKGFIGMASELVAADKKYADIVSQLLGAIIVVDHIDHALQIARKNNYLLRIVTLDGEY
                     LTPGGALSGGAFKNAGNLLSRRREIEELEESIRKSMKKADEISNAIEALKEERTAVKN
                     QMEQLKTRQQEQYLQQNTAQMNVDQAVKKKEETINGHKELEIENREIEQQIIDIGNSK
                     NEIKHDLEQSEQKEKDLEQQIAGYGKELETLRDEESRQVLQVTDLDLEAEKFKQKQEF
                     FQQNCSRIEAEISRLNGETEEISKKLALAEEDIVGKQENITQIEETINASVLTTDGHS
                     EALKQAVAEKEAFVLKQKGFFTEREKLSDEIASLDKEVFRLNSQKERVEEAQEAQISY
                     MWSEYEITLSAAASMRDEELTDTSSIRKNILRLKDKIRGLGDVNVNAIEDYKNLMERY
                     EFLKNQHDDLVKAEQTLTGIIDELDAAMRKQFEIKFKEIGREFDSVFKEMFGGGKGTL
                     ELSEDEDILEAGIRIIAQPPGKRLQNMMQLSGGEKALTAIALLFAIQNLKPSPFCMLD
                     EIEAA"
BASE COUNT          424 a          503 c          317 g          644 t
ORIGIN      
        1 gctgcctcga tctcatcaag catacaaaac ggagaaggct ttaaattctg aatggcaaat
       61 aacagcgcaa tcgcagtcag cgctttttcc ccgccggaca gctgcatcat attctgcagc
      121 ctttttccag gcggctgcgc aatgatcctg ataccggctt cgagaatatc ctcatcctct
      181 gaaagctcaa gcgtaccttt tccgccgccg aacatctctt taaataccga atcaaattcc
      241 ctgccgatct ccttgaattt tatttcgaac tgttttctca ttgcagcatc cagctcgtca
      301 ataataccag tcaatgtctg ttccgctttt acgaggtcgt catgctggtt ttttaaaaac
      361 tcatagcgtt ccatcagatt tttataatcc tctattgcat tgacattgac atctccaagc
      421 cctctgatct tatcttttaa tcgtaatata tttttacgga tggaagaagt gtctgtcagt
      481 tcttcatccc tcatggaggc tgctgcggaa agcgtgatct catattcact ccacatataa
      541 ctgatctgcg cctcctgcgc ttcttccaca cgttctttct ggctgttcag ccggaaaact
      601 tccttatcaa gagaagctat ttcatcggac agcttttctc tttccgtaaa aaatcctttt
      661 tgctttaaaa caaacgcttc tttttctgca accgcctgct taagcgcctc cgaatgacca
      721 tccgtcgtca atacggaagc attgatcgtc tcctctatct gagtaatatt ttcctgtttg
      781 ccgacaatat cttcttctgc cagtgccagt ttttttgaaa tttcttctgt ttccccattc
      841 aaacgactga tttcggcttc aatacgggaa cagttctgct gaaaaaattc ctgcttctgt
      901 ttgaattttt ctgcttccag atcaagatcg gtcacctgca atacctgtct ggattcttca
      961 tcacgcagtg tttcaagttc cttaccatag cctgcgatct gctgttcaag atccttttct
     1021 ttctgttccg actgctccag atcgtgcttg atctcatttt tgctgtttcc aatatcaatg
     1081 atctgctgtt caatttccct gttctcgatc tccagctcct tatggccgtt tatcgtttct
     1141 tctttctttt taacagcctg gtcaacattc atctgcgctg tattctgctg cagatactgt
     1201 tcctgctgac gcgtttttaa ctgttccatt tgatttttga ctgcagtccg ttcttctttt
     1261 aaagcttcga tcgcattgga aatttcatca gcctttttca tgcttttcct gatactttcc
     1321 tcaagctctt cgatttccct tcttctgctc agcagatttc cggcattttt aaaagctccg
     1381 cctgacaaag ctccgcccgg cgtcagatat tcaccgtcta acgtaacaat acgcagcaga
     1441 taattatttt ttctggctat ctgtaatgcg tgatcgatat gatctacaac aataatagct
     1501 cccagcagct gactgacaat atccgcatat tttttgtccg cggctacaag ctcggatgcc
     1561 attccgataa atcctttttc ttccctgatc tccggtctgc cgaactcacc gcgggctctg
     1621 acacttgtca atggaagaaa tgtcgcacgg ccaaacttat tttccttcag ataccggatc
     1681 atctcccttg caacctgctc gtcggctgtg acgatattct gaatattgct tccaagggct
     1741 gtttcaatag ctgtttcata ctttttttca acagaaataa tatcggcaac aacaccgatc
     1801 agccctttta tggaatcttt tcgttccata acactgcgga tgctgctccc ataaccatca
     1861 taacgttccg ttatattttt aagagctt
//
LOCUS       DAOSYE010000469         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51587_length_1886_cov_3.712179, whole genome shotgun sequence.
ACCESSION   DAOSYE010000469 DAOSYE010000000
VERSION     DAOSYE010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51587_length_1886_cov_3.712179"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PLU43_08180"
     CDS             <1..1339
                     /locus_tag="PLU43_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO28429.1"
                     /translation="PEMLFLFALIMILHLSIISHGKGVYMYTEDGREIIDASSGAVLV
                     SLGHGQQDMAEVLKKQAEKIAFAYRWDCITQVLENASRQVCEASEFDFTKVFFVCGGS
                     EATEIAMKLARRYFINIKKESKFKIISRRQSYHGSTMGALSITSFPARQNGYSEYLFE
                     QGHIPPAYCYRCWYGKECGSCAVECACALEEEILKQGPDTVAAFIMEPVSGMSLGAAH
                     GNQAYFKKIREICDKYEVLLIADEVMCGCGRTGEMYAYQNFGVKPDIIALGKALAGGY
                     FPIGAVACVQKIYDGIDANAAEFPPGYSWAGNPLGSAVVCKTFDMLKEKDLLPAVREK
                     GEYLKSELKALIPKHSIIGDVRGIGLMIGMEFVKNKETKESFDAGEKVTGLLSAAFAK
                     ENMFVETSTGCNRGTKGDMAMVAPAFIVTYEEMDEIVKRIDRAVASVEKKLGF"
     gene            1350..>1886
                     /locus_tag="PLU43_08185"
     CDS             1350..>1886
                     /locus_tag="PLU43_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOO28430.1"
                     /translation="MIENTFGKIQSVEEAMKHIEDHVRLMVGGFGGIGSPPLLCRAIF
                     EKNVQNITLICNDAGFPQVGVGPIVCNGQVKKMITTHIGSNPFAGRRMNEKLMEVEFI
                     PQGIFYEQIRAAGMGLGGILEDIGMETMIAAGKSKIELDGKEYILAPAISADVAVIYA
                     KKADPYGNLVYEKTARGAN"
BASE COUNT          582 a          305 c          520 g          479 t
ORIGIN      
        1 tccagagatg ctctttttgt ttgctctgat tatgatactt cacttaagta ttatatcaca
       61 tggaaaaggc gtctatatgt atacggaaga cgggagggaa attatagatg cttccagtgg
      121 tgctgtttta gtaagtcttg gacatggtca gcaggatatg gctgaagttt taaagaaaca
      181 ggcagagaag atcgcgtttg catatcgctg ggactgtatt acgcaggtgc ttgaaaatgc
      241 aagcagacag gtatgtgagg cttcagagtt tgattttaca aaagtctttt ttgtctgcgg
      301 cggttcggaa gcgacagaga tcgccatgaa acttgccaga agatatttta tcaatataaa
      361 aaaagaatca aaatttaaga tcatatcgag aagacagagc tatcatggca gtacgatggg
      421 ggcactttct attacaagct ttccggccag gcagaacgga tactcggaat atttatttga
      481 acagggtcat attccgcccg catactgtta cagatgctgg tacggaaagg aatgcggcag
      541 ctgtgccgtg gaatgtgcgt gtgcgttaga agaagaaata ttgaaacagg ggccggatac
      601 ggttgctgcg tttattatgg aaccggtgtc cggaatgtct ttaggtgcgg cacatggaaa
      661 tcaggcatac tttaaaaaaa taagagagat ctgtgacaaa tatgaggtgc tgctcattgc
      721 ggatgaagtg atgtgcggat gcggaagaac aggagagatg tacgcctatc agaatttcgg
      781 cgtaaaaccg gatattatag cgcttggcaa agcacttgcc ggcggctatt ttccaattgg
      841 agcggttgca tgcgtgcaaa aaatatatga cgggatcgat gcaaatgcag cggaatttcc
      901 gccgggttat tcatgggcag gaaatcctct tggctccgct gttgtatgta aaacgttcga
      961 catgctcaaa gaaaaggatc ttttaccggc agtcagggaa aaaggagaat atttaaaaag
     1021 cgagcttaaa gcattgattc caaagcattc gattatagga gacgtccgcg gaatcggttt
     1081 aatgatcggt atggaatttg tgaaaaacaa agagacaaaa gaatcatttg atgctggtga
     1141 aaaggttacg ggactgctgt ctgcggcatt tgcgaaagaa aatatgtttg tggaaacgag
     1201 tacgggctgt aatcgcggga caaagggtga tatggcaatg gttgcccctg cttttattgt
     1261 tacttatgaa gaaatggatg agatcgtaaa aagaatagac agggcggttg catcggtaga
     1321 aaagaaactg ggattttgag gtgtgaaagg tgatagaaaa cacatttggt aaaattcaaa
     1381 gtgtagaaga ggcaatgaaa catatagagg atcatgtaag gctgatggtc ggcggatttg
     1441 gaggtattgg ttcgccgcct cttctatgca gggcgatatt tgagaaaaat gtacagaaca
     1501 ttacactgat ctgtaatgat gcaggctttc cgcaggtagg agtaggaccg atcgtatgca
     1561 atgggcaggt taaaaaaatg atcacgacgc atattggttc gaatccgttt gccggcaggc
     1621 gcatgaatga aaaattaatg gaagtggaat ttatccctca ggggatcttt tatgaacaga
     1681 tccgggcagc aggcatggga ctgggcggaa ttttagaaga tatcggcatg gaaacgatga
     1741 tcgcggccgg aaaatccaaa atagaactgg atggaaaaga atatatttta gcaccggcca
     1801 taagcgcgga tgtagcggtc atctatgcta aaaaagcaga tccttacgga aatcttgttt
     1861 atgagaaaac agcccgggga gccaat
//
LOCUS       DAOSYE010000470         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51686_length_1884_cov_3.025697, whole genome shotgun sequence.
ACCESSION   DAOSYE010000470 DAOSYE010000000
VERSION     DAOSYE010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51686_length_1884_cov_3.025697"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..863)
                     /locus_tag="PLU43_08190"
                     /pseudo
     CDS             complement(57..863)
                     /locus_tag="PLU43_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
     gene            complement(879..1208)
                     /locus_tag="PLU43_08195"
     CDS             complement(879..1208)
                     /locus_tag="PLU43_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOO28431.1"
                     /translation="MKCQDMEQCIQLFIDNRLTGVKLQEFIEHIENCPVCYEEMETSY
                     LLKEALLRLEDGDAFDLHSELLEKLSTMKRCAAVHAFISVLRRTILIAAGMTLLAELI
                     YVYLVIF"
     gene            complement(1357..1677)
                     /locus_tag="PLU43_08200"
     CDS             complement(1357..1677)
                     /locus_tag="PLU43_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28432.1"
                     /translation="MIAGQEFSPACSCDMIMEEKAGKRMNAGIIELDLHGLRCEEAKK
                     KIDSTLNLSKGVYIIRLIHGYHGGTGIKRMVLEEYNYGREERVLRVCEGSNPGITELV
                     LREY"
     gene            complement(1678..>1884)
                     /locus_tag="PLU43_08205"
     CDS             complement(1678..>1884)
                     /locus_tag="PLU43_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28433.1"
                     /translation="TLSYPIYISATASGGVLNISFYSNSTATNGIAYEISVEGTDQKN
                     ATYIVGYLNGIEVSRVKAYTSSYK"
BASE COUNT          515 a          458 c          295 g          616 t
ORIGIN      
        1 atatacgaaa acaccatatg aaattataaa acatattgga tttaaaagca tactgccttc
       61 gtatatagat catcgatacg ggctggcttc agatcacaat cgattttcat atgggtatcg
      121 atcgttgcca gtgtcctgct taaatctgcc agatcaaaat gattttccaa tgtctctttt
      181 atactttttt ttgtgatatc cggaatatgt ttctttagat tttcaatatc atgatattga
      241 atgagcagac tggtcgcagt tttctgaccg atcttcggaa ctcccggaat attatcagaa
      301 ctatcaccca tcagcgcttt cagttcgata atctgcaggg gctctatacc gtatttttca
      361 ataacatcct ttgtataata atcttctgtt tccgttttgc cgccctttgt cttcgggatc
      421 cgtactttaa tatgatctgt agagacctga agcagatctc tgtctcctga tacaagagac
      481 acttccatct gctctttttc tgcgcgcttt gcaagcgttc ctaaaatatc atccgcttca
      541 tagcctgcct gctcaagaat cataatatcc atcgcctgca gcagctcttt taaaaccgga
      601 atctgttctc tcagttcatc cggcatcggt tttcttgtgc ctttataatc ctcaaaaatt
      661 ttatgccgga aagtcggtgc ttttacatca aatgcaacga tcagatattc tgcctgttcc
      721 tcatccagaa ttttaaacat aatatttaaa aatccataaa ttgcattcgt atgaaaacct
      781 gcggcattgg tcagatccgg aactccgtaa aatgcacggt ttgcaatgct gtttccgtct
      841 attaaaacta ttttctcact catttcccgg ctcccatcct aaaatattac caaatataca
      901 taaatcagct ctgccaaaag agtcattccc gcagcgatca ggatcgttct gcgaagaact
      961 gatataaaag catgaacagc agcacatctt ttcatggtac tcagtttttc caaaagctcg
     1021 gaatgcagat caaacgcatc tccatcctcc aaacgcagca gtgcttcctt taaaagatag
     1081 cttgtttcca tttcttcata acaaacagga cagttctcaa tatgctcgat aaattcctgc
     1141 agctttacgc ctgtcagccg gttatcaata aacaattgaa tgcattgttc catatcctgg
     1201 catttcataa agggctccct atcataattt atagtatctc aaaaaaagct ttttattcat
     1261 cagtattctt tttatactat acaccaaata tagcgccttc acaagctgtt tcaacgtatc
     1321 agcaccatat tttgatccat tccgtatgct cctgcatcaa tattctctta aaaccaattc
     1381 agtgattcct ggattacttc cctcgcagac tcttaaaact ctttcttcac gtccgtaatt
     1441 atattcctcc aaaaccatgc gcttgatacc agttccgcca tgatacccgt ggatcagtct
     1501 gattatgtaa acaccttttg acaaattaag cgtactgtct atttttttct ttgcttcctc
     1561 acagcgcagg ccatgcagat ctaattcaat gattcccgca ttcattctct ttcccgcctt
     1621 ttcttccata atcatatcac aagaacaggc aggagaaaac tcctgccctg cgatcactta
     1681 tttgtagctg ctcgtatatg cttttacacg gcttacctca atgccgttca gataaccgac
     1741 tatataggtc gcatttttct ggtccgttcc ttctacggaa atctcataag ctatgccatt
     1801 cgttgcggta ctgttactgt aaaaggatat attcagcaca ccgccggatg ctgttgcact
     1861 gatatagatc ggatacgaaa gcgt
//
LOCUS       DAOSYE010000471         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51866_length_1880_cov_2.581370, whole genome shotgun sequence.
ACCESSION   DAOSYE010000471 DAOSYE010000000
VERSION     DAOSYE010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51866_length_1880_cov_2.581370"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLU43_08210"
     CDS             complement(<1..451)
                     /locus_tag="PLU43_08210"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HOO28434.1"
                     /translation="MPDMHALLSASSSKQWLHCPPSVRLQEGFPNESSVYAEEGTFAH
                     EVCEYKVRKYLHERVKRPQSEEFYTEEIDQITDVYAEFVIGVIEQMKQNGCEPLVLVE
                     ERVDYSHIAPSGFGTADMLIIGKDENGKGLMHVCDFKTGQGVFVDTDH"
     gene            complement(467..934)
                     /locus_tag="PLU43_08215"
     CDS             complement(467..934)
                     /locus_tag="PLU43_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28435.1"
                     /translation="MAAEKQETTNVEPITISMKKLTAGIGTVFAGVLQMLEALEPQVA
                     KQLVSSVTEATDITPGEMPPVETDHRSNAADPQDAGKENAEPEPAAPEEASEEKQSTV
                     TLDDITKIIVRKVKQNRSNNEKIGAILKTYCVSKVSDLPAEKYEAFVTDLSQL"
     gene            complement(948..1406)
                     /locus_tag="PLU43_08220"
     CDS             complement(948..1406)
                     /locus_tag="PLU43_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28436.1"
                     /translation="MEYGTERVRYPGQYGSREHYNRIVFICSPYRPEAEEPEEKADEL
                     LRNIHLARLGCKTAVRRGCTPIAPHLIFPQFLKDEKPEDREAGMKMGVQLLLLCDEVW
                     VLGRRISEGMGNEIAIATEEGIPIFILENPASSEERLLKAIFGAKAHDEG"
     gene            complement(1568..>1880)
                     /locus_tag="PLU43_08225"
     CDS             complement(1568..>1880)
                     /locus_tag="PLU43_08225"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO28437.1"
                     /translation="YDVDDNGDKDGDAEKAMEQSYQREYSWREDRIGESPETVYIRKE
                     TREEMLGRMTDRQREVFLLYYRDGFNQREIAQLLGISQQKVSKRLSAALANAKRNFDF
                     F"
BASE COUNT          368 a          572 c          417 g          523 t
ORIGIN      
        1 tgtgatctgt atccacaaaa acgccctggc cggtcttgaa atcgcagaca tgcatcaggc
       61 ctttgccgtt ttcatctttc ccgataatca gcatatccgc cgtcccgaaa ccggatggcg
      121 cgatatgact gtaatctacc cgctcttcca ccagcaccag cggctcacag ccgttctgtt
      181 tcatctgttc gataacgccg atcacgaatt ccgcatacac atcggtaatc tggtcgatct
      241 cttccgtgta gaattcctcg gactgcggcc gctttacccg ctcatgcaga tattttctga
      301 ctttatattc acagacctcg tgggcaaacg tgccctcctc ggcatacacg gaagactcat
      361 tcggaaagcc ctcctgcagc cgtaccgaag gcgggcaatg gagccattgt tttgagctcg
      421 atgcactaag cagcgcgtgc atatcaggca tgcttccacc tccccgttag agctgtgaga
      481 gatctgtcac aaacgcctca tatttttccg caggaaggtc acttactttg gacacacagt
      541 aagtcttgag gattgcgccg atcttctcat tattggagcg gttctgtttc accttccgga
      601 cgatgatctt cgtgatatca tccagagtga ccgtgctctg tttctcctcg ctggcttctt
      661 ccggtgccgc cggttccggt tcagcgtttt ctttacccgc atcctgcgga tctgctgcat
      721 ttgatctgtg atctgtttcc acaggcggca tttcacccgg cgttatatcc gtcgcctccg
      781 tcaccgaact caccagctgt ttcgccacct ggggctccag tgcttccagc atctgaagca
      841 ctccggcaaa tactgtcccg atcccggccg tcaacttctt catgctgatc gtgatcggct
      901 ctacgttggt agtttcctgt ttttctgcag ccatgcgcgt ctcctcctta tccttcatcg
      961 tgtgccttgg ctccaaagat cgccttcagc agccgctctt ctgagctggc cgggttttcc
     1021 aggataaaaa tcggaattcc ctcctctgtt gcgatggcga tttcattgcc catgccttca
     1081 ctgattctcc ggcccaagac ccaaacttca tcgcacagga gaagaagctg tacacccatc
     1141 ttcattccgg cctcccgatc ttccggcttc tcatccttca aaaactgtgg gaagatgaga
     1201 tgcggggcaa ttggtgtgca tccacgacgg actgccgtct tgcacccaag gcgggcgaga
     1261 tgaatgtttc gaagcagttc gtctgccttc tcttcaggct cctccgcctc tggacggtac
     1321 gggctgcaaa tgaaaacgat ccggttataa tgttctctgc ttccgtactg tccggggtat
     1381 ctcacacgct ctgttccata ttccatactt atgtcctttc tctgcaggat tggcctgcag
     1441 tccctaaatg taaaaggcga ctggtacctt ccgtcttgga aagcaccaac cgcctcgttg
     1501 tcaggaggac ccttgatcaa gtcctctcat atactgtcgt gggatggtct cttttacacc
     1561 cagcctctca aaaaaaatca aaatttcttt ttgcgttcgc cagagcggct gacagacgct
     1621 tgctaacctt ctgctgcgaa ataccgagca gctgggcgat ttcccgctga ttaaaaccgt
     1681 ctctgtaata cagaaggaag acctctctct gcctgtctgt catgcggccc agcatctctt
     1741 cgcgggtttc tttccgaatg tagactgtct ccggactctc tccaatccgg tcttcacgcc
     1801 agctgtattc ccgctgataa gactgctcca tggctttttc agcatcgccg tccttgtcgc
     1861 cattatcatc cacgtcatag
//
LOCUS       DAOSYE010000472         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_51905_length_1879_cov_3.131579, whole genome shotgun sequence.
ACCESSION   DAOSYE010000472 DAOSYE010000000
VERSION     DAOSYE010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51905_length_1879_cov_3.131579"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..906
                     /gene="murI"
                     /locus_tag="PLU43_08230"
     CDS             85..906
                     /gene="murI"
                     /locus_tag="PLU43_08230"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOO28438.1"
                     /translation="MNNYNAPIGVFDSGVGGLTVAKEIMTQIPNERIVYFGDTARVPY
                     GSKSKKTITKYTRQIIRFLMTYDVKALVIACNTMSANALEEVEKELSIPIIGVVKPGA
                     RVAASQTKNRRIGVIATESTIHSEMYEHFINQIDPSLQVIGKACPLFCPLVEEGMLDD
                     PVTDEIVRRYLTGLMEQNVDTLILGCTHYPLLAPAIGRIVGEKVRLVNPAYETAKELR
                     GLLELKNLLNQDPAEDSDSQYRFYVSDLADKFSNFAANILHNDNLTTAQVDIEKY"
     gene            925..1371
                     /locus_tag="PLU43_08235"
     CDS             925..1371
                     /locus_tag="PLU43_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOO28439.1"
                     /translation="MTKDVIVSISGLQFGNDMENDKIEIITSGNYYKKNNKHYVLYEE
                     ITEGFEGVTKNIVKFDERVFNLTKSGITNVNMVFEKDKRSITNYITPYGNILVGIDAN
                     KIDVRESNEKIQINIDYALDVNYEHLADCKIHMDITAKNEGAFSLH"
     gene            1391..>1879
                     /locus_tag="PLU43_08240"
     CDS             1391..>1879
                     /locus_tag="PLU43_08240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOO28440.1"
                     /translation="MNVRKAIIPAAGLGTRFLPATKAQPKEMLAIVDKPTIQYIIEEA
                     VASGIQDIIIVTGRNKRSIEDHFDRSIELELELEKSGKMEMLEMVRKISDMANIHYIR
                     QKEPRGLGHAILTAKHFIGNEPFAVLLGDDVVVSKKPCLSQMLDVYNEYKTSVLGVQK
                     VAK"
BASE COUNT          622 a          328 c          484 g          445 t
ORIGIN      
        1 gtgactgcat cttgcaatat ttccggatta aggatagaat aaataagcac tttgtgtgcg
       61 atatgcaaaa aagaggtacg aaaaatgaat aattataatg cgccgatcgg tgtgtttgat
      121 tccggggtcg gcggtttgac ggtcgcaaag gaaattatga cgcagatccc gaacgaaagg
      181 atcgtatatt tcggagatac ggcaagagtt ccgtatggca gtaaatccaa aaaaacgatc
      241 actaaatata caagacagat catccgtttt ctgatgacat atgatgtaaa ggcacttgtg
      301 atcgcttgta atacaatgag tgccaatgcg ctggaagaag tcgaaaagga gctgtcgatc
      361 ccgatcatcg gcgtggtaaa accaggggca cgggtcgcgg catcacagac aaaaaatcgg
      421 cgtatcgggg tgattgccac agaaagtacg atacacagtg aaatgtatga gcattttatc
      481 aatcagatcg acccgtcgct gcaggtgatc ggaaaagcct gtccgttgtt ctgcccgctt
      541 gtggaagaag ggatgttaga tgatcccgtt acggatgaaa ttgtacggcg gtatctgacc
      601 ggtcttatgg agcagaatgt ggatacactg attttaggct gtacacacta tccgctgctt
      661 gcaccggcga tcggaaggat cgtcggagaa aaggtcagac tcgtaaatcc ggcatatgag
      721 acagcgaaag aactgagggg gcttttagaa ctgaaaaatc tgctgaatca ggatccggcg
      781 gaagacagtg acagtcaata caggttctat gtcagtgatc ttgcggacaa gttcagtaat
      841 ttcgcggcaa atattctgca taatgacaat ttgacgaccg cgcaggtaga cattgaaaaa
      901 tattgataga ccggaggctt ttttatgaca aaggatgtaa ttgtttccat atccggactc
      961 caatttggga atgatatgga aaatgataaa atagaaatta taacgagtgg aaattattat
     1021 aaaaaaaata ataagcacta tgtactttat gaggaaataa cggaagggtt tgaaggagtg
     1081 acaaaaaata tcgtcaaatt tgacgaacgg gtattcaacc tgacaaagag cgggatcacg
     1141 aatgtaaaca tggtgtttga aaaagataaa cgcagtatta cgaattatat tacaccatat
     1201 gggaatatcc ttgttggaat cgacgcgaac aagatcgatg tcagggagtc gaatgaaaaa
     1261 atacagatca atatagatta tgcgctggat gtgaattatg agcatttggc ggattgcaaa
     1321 atccatatgg atattacggc aaaaaatgag ggagcgtttt cattacactg agccaaagga
     1381 ggatacaaac atgaatgtaa gaaaggcgat tattccggca gcaggacttg gaacaagatt
     1441 tttgccggct acaaaggcac agccaaagga gatgcttgcg atcgttgaca aaccgacgat
     1501 ccagtacatt attgaagaag cggttgcttc cggaattcag gacattatta ttgtaacggg
     1561 aagaaacaag cgatccatag aagatcattt cgaccgttcg atcgagctgg aactggagct
     1621 ggagaagagc ggaaagatgg aaatgctgga gatggtccgc aaaatatcgg atatggcaaa
     1681 tattcattac atcagacaga aggagccaag aggactggga catgcgattc tgaccgcaaa
     1741 gcattttatc ggaaatgaac cgtttgccgt actgctcggc gatgatgtgg tcgtatcaaa
     1801 aaaaccgtgc ctaagtcaga tgctggacgt ttacaatgaa tacaaaactt ccgtactggg
     1861 cgtgcagaag gtagccaag
//
LOCUS       DAOSYE010000473         1876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52048_length_1876_cov_2.557386, whole genome shotgun sequence.
ACCESSION   DAOSYE010000473 DAOSYE010000000
VERSION     DAOSYE010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52048_length_1876_cov_2.557386"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLU43_08245"
     CDS             complement(<1..514)
                     /locus_tag="PLU43_08245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO28441.1"
                     /translation="MKIRHITKTEAAKVRKLSSVCFDYPYDTKGMTDEAYTEYLMENP
                     QETLHAHPECTIGAFSDNGELMAAAAAIPFSFYFDGRSYEGNGIGNVSTYPQYRRKGA
                     VRAIFKQMLEEAYQSGSAFSYLYPFSEAFYNAFGYYRMDNSICHQYALHSLPDYSFTG
                     SFSIKEGAHVP"
     gene            complement(543..1346)
                     /locus_tag="PLU43_08250"
     CDS             complement(543..1346)
                     /locus_tag="PLU43_08250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO28442.1"
                     /translation="MKEVKLLLTDLDDTLLTDEKQITKRTLKAFADLKKSGILTGIAT
                     ARSEIPAAPYVSALKPEVLILNSGGLVIVNGKTVSKTLLNGKVTAQVLSLVCREDSVM
                     DICAETEDGYYCNQKDIFKFGADYSHAKFHDFIKPFEKEIYKLTIHSKSPENLQAITE
                     QFPECRFFCYAASGWCCIQPEGVCKGKGAEIAAAHLGIPFSRVAAFGDDITDLELLRD
                     AGCGVAMANAIPVIEKAARYHTGSNEEDGVAQFIEDYILIRDEKDGLAG"
     gene            complement(1385..>1876)
                     /locus_tag="PLU43_08255"
     CDS             complement(1385..>1876)
                     /locus_tag="PLU43_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28443.1"
                     /translation="QVVYDESFQAFSPLEEQTVLVLVPYASEAASVKFAINRMRQEGS
                     LGEFYTEYVVYQDSELSDEEIKEKIKTADYMILGSEEDLGLDSDAAKEAAEAAMSFGK
                     EAVTAVLCTGLPYQTELYPDLPCIVCYGETGMSEEDAKSGVITGRYGPNIPAAVETIF
                     GVD"
BASE COUNT          493 a          497 c          345 g          541 t
ORIGIN      
        1 cgggaacatg cgctccttct tttatggaaa agcttccggt aaaggagtaa tccggcagac
       61 tgtgcagggc atattgatga catatggaat tatccattct gtaatagccg aatgcattat
      121 aaaaagcttc gctgaacgga tacagataag aaaaagccga tccgctttga taagcttctt
      181 caagcatctg cttaaaaatt gcccggacag ctccctttct cctgtactgc ggataagtgg
      241 aaacattgcc gatcccgttt ccctcataag atcttccgtc aaaataaaaa gaaaacggaa
      301 tcgctgcagc cgcagccatc agttcgccgt tatcactgaa tgcgcctatg gtacattccg
      361 gatgcgcatg cagcgtttcc tgcggatttt ccatcagata ttccgtatac gcttcatctg
      421 tcattccttt tgtatcataa ggataatcaa aacaaacgga agaaagcttc cgtaccttag
      481 ctgcttctgt tttcgtaata tgcctgatct tcattcacag accctcccat ttttcctgct
      541 gatcagccgg ccaaaccatc tttttcatcg cggatcaata tatagtcttc aataaactgt
      601 gccactccgt cctcttcatt agatcccgta tgatatctgg ccgccttttc tataacagga
      661 attgcattcg ccatcgcgac gccacaccct gcgtcccgta aaagctccag atccgtaata
      721 tcatccccga atgccgccac acgcgaaaat ggaatgccaa gatgcgccgc cgcaatttca
      781 gcgccttttc ctttacagac accctccggc tggatgcagc accagcccga agccgcatag
      841 cagaagaaac ggcactccgg aaactgttcc gtgatcgcct gcaggttttc aggacttttt
      901 gaatggatcg tcagcttata tatttccttt tcaaacggtt ttataaaatc atgaaacttt
      961 gcatgggaat aatccgcacc aaatttaaaa atatctttct gattacaata atatccgtct
     1021 tctgtttcag cacagatatc catcacggaa tcttctctgc agacaagaga aagaacctgt
     1081 gcggtaactt ttccgtttaa cagtgttttg ctgacagttt ttccattcac gatcacaaga
     1141 ccaccgctgt tcaaaatcaa gacctccggc ttcagggcac ttacatatgg tgccgccgga
     1201 atctcgctgc gtgctgtcgc aattcctgtt aaaataccgg attttttcaa atcggcaaag
     1261 gccttcagtg ttcttttcgt tatctgtttc tcatctgtca gcagcgtatc atccagatct
     1321 gtcagcagca attttacttc tttcattatc atctctccaa ataaaatatt attaaatgtg
     1381 tttgttaatc aactccaaat atagtttcaa cagcagccgg aatatttgga ccataccggc
     1441 cggtgatcac accactcttt gcatcttcct ccgacatacc ggtctctcca tagcagacga
     1501 tgcatggaag atccggataa agctccgtct gatagggaag tcctgtgcac aaaaccgctg
     1561 ttaccgcttc ttttccaaaa gacatggcag cttcagcagc ttcttttgca gcgtcactgt
     1621 ccagtccgag gtcttcttca cttccaagaa tcatataatc cgctgtcttt attttctctt
     1681 tgatttcttc gtcagacagt tcagaatcct ggtaaacaac atattccgta taaaattccc
     1741 cgagacttcc ttcctgccgc atcctgttta ttgcaaactt aacgctcgcc gcttccgatg
     1801 catatggcac caggacaagg actgtctgtt cttccaaagg cgaaaatgcc tgaaaacttt
     1861 cgtcgtaaac aacctg
//
LOCUS       DAOSYE010000474         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52082_length_1875_cov_3.616484, whole genome shotgun sequence.
ACCESSION   DAOSYE010000474 DAOSYE010000000
VERSION     DAOSYE010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52082_length_1875_cov_3.616484"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1177)
                     /locus_tag="PLU43_08260"
     CDS             complement(68..1177)
                     /locus_tag="PLU43_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28444.1"
                     /translation="MTKAIIRMENVCKDYGENRVVKSLNMDVFEGEFLTMLGPSGCGK
                     TTTLRMLAGFEKPTAGNIYVEDKEIQEVEPYHREVNTVFQNYALFPHMTVYENIAFGL
                     QIKKVSKTEIRKKVEEMLELVQLIGYENRKPSQLSGGQKQRVAIARALINQPKVLLLD
                     EPLGALDLKLRKQMQFELKRLQKKLGITFIYVTHDQEEALTMSDRIAILSEGVLEQLG
                     RPRDIYNKPASKFVADFIGESNLFYGTVKSRQGNKIEVTLETGSVSADCETAGIGDIV
                     DISVRPENLKMSVTPVEGFHCPGVVKEYVFIGNIHKVVVLLASGKELRINQSPKVAEL
                     PAGTAVNLYWDSEDAVVIPSADNQVFQAVENPRRD"
     gene            complement(1273..>1875)
                     /locus_tag="PLU43_08265"
     CDS             complement(1273..>1875)
                     /locus_tag="PLU43_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO28445.1"
                     /translation="DITGYKDLLNSDYKDSIVIIDDARAVVGMASMAEGYDINDMSDE
                     ALAAASDYLEQLKPNIHAFNGDSPKTLMINGECPLGLIYGAECALAMDENPAIVGVYP
                     EEGVYFGFDMLSIAKEGKNKENGYKLIDYILDGKNSALISTVFPYFNPNTAALEYLDD
                     TYKDNPIKNMPEEERSKIVYLKDIGDDMTKVVDVWNNLKN"
BASE COUNT          452 a          508 c          346 g          569 t
ORIGIN      
        1 gataccggtc ctaccgtgcc ccagaaagta aagtttattt ttctcttttt catctgtcac
       61 cctcccttca gtccctgcgt ggattttcca ctgcctgaaa tacctgatta tccgcggaag
      121 gaatgacgac agcatcttcg ctgtcccaat aaagattgac cgccgtgccc gcaggcagct
      181 ctgcgacctt tggagactga ttgatccgca gctccttgcc gcttgccagc aatacgacta
      241 ccttgtgaat attcccgata aacacgtatt ccttgacaac accaggacag tgaaaccctt
      301 ccaccggtgt aacggacatt ttcagatttt ccggacggac tgaaatatcc acgatatctc
      361 cgataccggc cgtttcacag tctgcggaaa cgcttcctgt ttccagcgtc acttctattt
      421 tatttccctg tctgcttttt accgtcccgt agaacaaatt ggattctccg ataaaatcag
      481 caacaaattt ggaagctggt ttattataaa tatctcttgg cctgccaagc tgttccagta
      541 ccccttccga aaggatcgct atccggtcgc tcatcgtcag tgcctcttcc tggtcatgtg
      601 ttacataaat aaacgtgatc ccaagctttt tctgcaaacg cttcagttcg aactgcatct
      661 gcttgcgaag cttcagatcc aatgcaccaa gcggttcgtc cagcagcagg actttgggct
      721 ggttgatcag cgcccttgcg atcgccacac gctgcttctg accgccggaa agctggctcg
      781 gcttgcggtt ttcatagccg atcagctgta caagctccag catctcttcc acctttttac
      841 ggatctctgt ttttgaaacc ttcttgatct gcagcccgaa agcaatattt tcatagaccg
      901 tcatatgcgg aaataaagca taattctgaa acaccgtatt gacttctctg tgatacggct
      961 ccacctcctg gatctccttg tcttcaacat aaatatttcc ggctgtcggc ttttcaaatc
     1021 ctgcaagcat ccgcagtgtc gtcgttttac cgcatcccga aggtccgagc atcgtaagaa
     1081 attctccttc aaaaacatcc atattcaagg atttgacaac tctgttctct ccataatcct
     1141 tacatacatt ttccattctg ataattgctt ttgtcataaa aatgttcccc gctttctttt
     1201 tcagtttatt gcagaaagga caagaatgtg tctgccctat ccttgtcctt ccaccctctg
     1261 aaaatcgaaa ttttagttct taagattgtt ccagacatcc acgacctttg tcatatcgtc
     1321 tccaatatct ttcaaataca cgatcttgct tctttcctct tcaggcatgt tcttgatcgg
     1381 attatcttta tatgtatcat ccagatattc cagtgctgcc gtattcggat tgaaataagg
     1441 gaaaacagta gagatcagcg cgctgttctt tccgtccaga atataatcga tcagcttata
     1501 gccgttttcc ttgttctttc cttcttttgc gatagaaagc atatcaaagc caaagtatac
     1561 accctcttcc ggataaacac ctacaattgc aggattctca tccattgcaa gtgcacattc
     1621 cgcaccgtag atcagtccga gcggacattc tccgttgatc ataagcgtct ttggtgaatc
     1681 gccgttgaat gcatggatat tcggctttaa ctgctccaga taatcggagg ctgcggcaag
     1741 tgcctcatca ctcatatcgt tgatatcata tccttccgcc atactggcca taccgactac
     1801 agccctggcg tcgtcgataa tgacgatcga atctttataa tccgaattaa gcagatcctt
     1861 atagcctgta atgtc
//
LOCUS       DAOSYE010000475         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52130_length_1874_cov_3.456295, whole genome shotgun sequence.
ACCESSION   DAOSYE010000475 DAOSYE010000000
VERSION     DAOSYE010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52130_length_1874_cov_3.456295"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLU43_08270"
     CDS             <1..457
                     /locus_tag="PLU43_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28446.1"
                     /translation="EEADAQTMLLLLQLRDIASKRQCLFNITSEMNSIENQKLSQVAK
                     VNDFVVGSSITNLIVTQISENRSLAALFAELLDADGSEIYMKPASDYVKLETPVNFYL
                     LTEIAKIRREIVIGCKQYIDGEIRILVNPAKSLSVSFHKEDTLIVLALE"
     gene            complement(466..882)
                     /locus_tag="PLU43_08275"
     CDS             complement(466..882)
                     /locus_tag="PLU43_08275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOO28447.1"
                     /translation="MTDREILLQVIEENEYMRLLGLEMLQLEKGYGKGRMPYKKMLTN
                     PYGMLHGGSLYSLADIVCGTVASMSGQYVTTVNGSMNFMQPAINTEYVYCEAKAVREG
                     EHLVVFDVLIKADDGRILDNGSFTFFYTGRYVSQKE"
     gene            1021..>1874
                     /locus_tag="PLU43_08280"
     CDS             1021..>1874
                     /locus_tag="PLU43_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO28448.1"
                     /translation="MKLLNLLKKIMIPALLVWICIPLKTSASEYGNLLTEPYLQCPTD
                     TTVNVVWFTKSATADNYVLYGNRLSQKAYADTSRLSRIRTFDNQPVNIYRHEAVVTGL
                     PLNKTGNERVPYQAVSDGSKSGKYYLQAQEQAEAPMKILFTSDCQLKEMSAANYEKAV
                     ETAGHIDAVFYAGDLVNIPDQADEWFPLENSNAFFSLLQGSADQQIGSTVYHGGSILQ
                     EAPIYTAPGNHEFMGRTSTIFDLGLQFNDTRPAKYGDSDNTFNTITYEELFDIPSDQE
                     KLYYAVTIG"
BASE COUNT          544 a          450 c          369 g          511 t
ORIGIN      
        1 tgaagaggct gatgcacaga caatgctgct gctgttacag ctgcgtgata ttgcatccaa
       61 acggcaatgc ctgtttaata ttacaagcga aatgaattcc atagaaaatc aaaagctttc
      121 gcaagtcgca aaggtcaacg attttgttgt cggcagttcc attacaaatc ttatcgtcac
      181 gcaaatatcc gaaaatcgtt ctcttgcagc tttatttgca gaactcctgg atgcggacgg
      241 ttccgagatt tatatgaaac ccgcctcaga ctatgtcaaa ctggagactc ctgtcaattt
      301 ctatctgctg acggaaatcg ctaaaatacg ccgggaaatc gtaattggct gcaaacagta
      361 tatagacgga gaaatcagaa ttcttgtgaa tccggctaaa tcactgtctg tttcttttca
      421 taaagaagac acactgatcg tccttgcgct tgaatagtct ttgtattact ctttttggga
      481 aacgtaacga cccgtataaa aaaatgtaaa actgccgtta tcgagtatcc ggccgtcatc
      541 tgccttaatc agcacatcga atacgaccag atgctctcct tctctcactg cttttgcttc
      601 acagtataca tattccgtat taatggccgg ctgcataaaa ttcatactgc cgtttaccgt
      661 agttacatat tggccgctca tggaagcaac tgttccgcag acgatatccg ccaatgaata
      721 cagacttccg ccgtggagca tgccgtacgg atttgtaagc atttttttat aaggcatcct
      781 gccctttcca tagccctttt caagctgcag catttccagc cctaaaagac gcatatattc
      841 gttttcctct ataacctgca ataatatttc tctgtcagtc atggtcagtt tctcctttgt
      901 aagatacatt ccaatatacc ttaccaaaat ttatgaaaat aagcaaacac tctgtccatg
      961 atctcaaaat aatggtacta tgaaatcact taataaaaca gaattccatt tggagcatct
     1021 atgaaattac tcaatctgct gaaaaaaata atgatccccg cattgcttgt atggatctgc
     1081 atcccgctta aaacttccgc atccgagtac ggaaaccttc tgactgagcc atatttacaa
     1141 tgcccgaccg atacgacggt gaacgttgtc tggtttacga agagcgcaac tgccgataac
     1201 tatgttctat acggaaaccg gctgtcgcaa aaagcctatg cagacacaag ccggctgtca
     1261 cgcatcagga cctttgataa tcagccggta aacatctacc gccatgaagc ggtcgtaaca
     1321 ggactccctc ttaataagac cggtaacgaa agagtcccct accaggctgt cagtgacggg
     1381 agtaaaagcg gaaaatatta tttgcaggca caggagcagg cggaagctcc catgaaaatc
     1441 ctttttactt ccgactgcca gctaaaggaa atgtccgctg ccaattatga aaaagcagtc
     1501 gaaacagccg gacatatcga tgctgttttt tatgccggtg accttgtaaa catccctgat
     1561 caggcagacg aatggtttcc tcttgaaaat tcgaacgcat ttttttcgct tctgcagggc
     1621 agtgccgacc agcagatcgg cagcactgtt tatcacggag gctccattct gcaggaagct
     1681 cccatatata ctgctcctgg taatcacgaa tttatgggcc gtaccagcac gatttttgac
     1741 cttggtctgc agttcaatga tacgagacct gctaaatacg gcgatagcga caatactttt
     1801 aatacgatca cctatgaaga actgtttgat attccatccg accaggaaaa actatattac
     1861 gcggtaacca tcgg
//
LOCUS       DAOSYE010000476         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52182_length_1873_cov_2.960396, whole genome shotgun sequence.
ACCESSION   DAOSYE010000476 DAOSYE010000000
VERSION     DAOSYE010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52182_length_1873_cov_2.960396"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLU43_08285"
     CDS             complement(<1..616)
                     /locus_tag="PLU43_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HOO28449.1"
                     /translation="MDNEEPKKSGNAAMLKICGFIVDKRNLFFLLFGLLMIFSVIARS
                     WVNVENVISYYLPESTETKQGLELMDEEFTTYGTCKVMIANVSFEQADKIRSQIENID
                     GVFSVDFDDSEDHYNNGSALYGVTFDYDEDDEECLTPLNDLKQQLSGYDMYLTTSLGD
                     TQSELIAQEMGVISIIVAVIVVTVLLLTSQAYAEVPVLLLTFGSS"
     gene            complement(619..1398)
                     /locus_tag="PLU43_08290"
     CDS             complement(619..1398)
                     /locus_tag="PLU43_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO28450.1"
                     /translation="MRIDRERVFEAFALYTGRYDSGDEKIKLKIDHTYRVSELCERIA
                     KAQGLTEEETEISWLTGMLHDIGRFEQLRNYNTFNDALSVNHAQYGADILFLEGKIKE
                     YISDFSENELIEKVIRLHNTYRLPDSLSKRERVLSEILRDADKIDILKVNVEVPLEEI
                     YNITTKELRNAAVTDEVMESIREGHATVRSLKRTAADHLVGHISLVFELVFPVSFRIV
                     KEQGYLEKLMEFQSENRETQLRFEEIRKLMKRFMEERTGEQ"
     gene            complement(1480..>1873)
                     /locus_tag="PLU43_08295"
     CDS             complement(1480..>1873)
                     /locus_tag="PLU43_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOO28451.1"
                     /translation="SALVLYARSAMEGMRTGYFSYLAHPDLMFINDFGMDDNCRKAIS
                     IIIDEAEKKDYILELNANGFRRGKWKFAEGVRYPYPADAFWEEAAKAGIRVIIGSDCH
                     SPCKLNDEYILETEQYAKKLGLQMIDRI"
BASE COUNT          482 a          503 c          327 g          561 t
ORIGIN      
        1 ccgaagatcc gaatgtcaat aggagcaccg gtacttccgc ataggcctgc gatgtcagaa
       61 gcagaacggt aacgacgatc accgccacga ttatggaaat aacgcccatc tcctgtgcga
      121 tcagttccga ctgcgtatct ccaaggctcg ttgtcagata catgtcataa ccgctaagct
      181 gctgcttcag atcattgagc ggcgtcagac attcctcatc gtcctcgtca taatcaaagg
      241 taacaccata cagcgccgaa ccgttattat aatgatcttc actgtcgtca aagtctactg
      301 aaaacacacc gtctatattc tcgatctggc tgcggatctt gtctgcctgt tcaaaggaga
      361 cattggcaat catcacttta cacgttccat aagtggtgaa ttcctcatcc atcagttcca
      421 gtccctgttt tgtttctgtt gattccggca gataatagga tattacattc tcaacattta
      481 cccagctgcg cgcgattacc gaaaaaatca tcagcaatcc aaacagcagg aaaaaaagat
      541 ttctcttatc cacgataaat ccgcatattt tcagcattgc cgcatttccg cttttctttg
      601 gctcttcatt gtccattttt attgttcgcc tgtcctttcc tccataaagc gtttcatcaa
      661 tttcctgatc tcctcaaacc gaagctgtgt ttccctgttt tccgactgaa attccatcag
      721 tttttcaaga tatccctgtt cttttacgat ccgaaaactg accggaaaca caagctcaaa
      781 aacaagtgaa atatggccga caagatgatc ggccgccgtt cttttgaggc tcctgaccgt
      841 tgcatgccct tccctgatgc tctccattac ctcatcggtc accgcagcgt ttcgcagctc
      901 ttttgtcgtt atattataga tctcttccag cgggacttcc acgttgactt ttaaaatatc
      961 gatcttatcg gcgtcccgta aaatctccga caaaacccgt tcccttttgc tgagggaatc
     1021 cggcagccgg taggtattgt gcagacgtat gactttttcg atcagttcat tttctgaaaa
     1081 atccgaaata tactctttga tctttccttc taaaaacaaa atatcggcac catactgtgc
     1141 atggttaacc gataatgcgt cattaaacgt attgtaattt cttaattgtt caaacctgcc
     1201 gatatcgtgc agcatccccg taagccagga aatttccgtc tcctcttctg ttaatccctg
     1261 cgccttcgct atcctctcac acagttcact gaccctgtaa gtatggtcga tcttcagttt
     1321 gatcttctcg tcgccgctgt catatctgcc cgtatataat gcaaatgcct caaaaacacg
     1381 ctctctgtct attctcatct gactcactcc aaaatgcttt tttaaaaatc tattttgcat
     1441 tttatcaaac tctgtgaaaa aaagcaacac atgaattctc taaatcctgt caatcatctg
     1501 caagccaagt ttttttgcat actgttctgt ctctaaaata tattcgtcat ttaatttaca
     1561 cggcgaatga caatccgaac cgataatgac cctgataccc gcttttgcag cctcctccca
     1621 aaaggcatcg gccgggtatg gatagcgaac tccttccgca aacttccatt tcccgcggcg
     1681 gaatccgttc gcattcagct ccagtatata atcctttttt tccgcctcgt caataatgat
     1741 ggagattgcc ttcctgcagt tgtcatccat tccgaaatcg ttgatgaaca tcaggtccgg
     1801 atgtgccaga taagaaaaat atcctgtcct catcccctcc atggcagaac gcgcatataa
     1861 aacaagcgcg gag
//
LOCUS       DAOSYE010000477         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52188_length_1873_cov_2.848735, whole genome shotgun sequence.
ACCESSION   DAOSYE010000477 DAOSYE010000000
VERSION     DAOSYE010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52188_length_1873_cov_2.848735"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..344
                     /locus_tag="PLU43_08300"
     CDS             6..344
                     /locus_tag="PLU43_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOO28452.1"
                     /translation="MFGEVRIADDVVANIAGLAATEIEGVAATAGNITNELMSKVGMK
                     TLPKGVKVELLDDVVSVEMALVMEYGYNIPATCRKVQERVKSSIENMTGLEVSDVNIR
                     IAGIDMTKQS"
     gene            359..790
                     /gene="nusB"
                     /locus_tag="PLU43_08305"
     CDS             359..790
                     /gene="nusB"
                     /locus_tag="PLU43_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO28453.1"
                     /translation="MTRSEIREHIFKLLFRIEFNAAEDMPSQIRLYFEDPVNAEEDDR
                     KKAGWIQEKDERYIREKYLKIADKIPELDSLIDHAAKGWKASRLGKVDLTILRLAVYE
                     VKFDEDIPVGVAIDEAVELAKKFGQDESPAFINGILAKIAM"
     gene            799..>1873
                     /gene="xseA"
                     /locus_tag="PLU43_08310"
     CDS             799..>1873
                     /gene="xseA"
                     /locus_tag="PLU43_08310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO28454.1"
                     /translation="MKSVYSVGQINSYIKNMFSQDFMLRSIYVKGEVSNVKYHSSGHI
                     YFTMKDKSGTIACVMFAGYRKGLDFTLKEGQQVIAGGSIEVYERDGRYQLYAKELIRD
                     GAGLLYERFEELKKELEEMGMFSAQYKKPIPKYAKKIGIVTAPTGAAVRDIINVAKRR
                     NPFVQLILYPALVQGDGAKESIVNGIHALELAGVDVIIAGRGGGSIEDLWAFNEEIVA
                     RAIFECSIPIVSAVGHETDTTIADFVSDMRAPTPSAAAELTVFSYEDFTRRCLDGRAM
                     LRHGFDRRLTEQKKLYEQRQLRLSLVSPQRRIHEKRMHCAGLTERLEKRMQDLLIYKK
                     HRLQLQIERMKALSPLERLQNGYA"
BASE COUNT          541 a          332 c          549 g          451 t
ORIGIN      
        1 aggacgtgtt cggtgaggtt cgtatcgccg atgatgtggt tgccaatatt gccggccttg
       61 cagctacgga gatcgaaggg gtagcggcta ccgcaggcaa tattacgaac gagctgatga
      121 gtaaggtcgg aatgaaaaca ttgccaaagg gcgtgaaggt cgaactgctg gatgacgttg
      181 tttctgttga gatggcgctt gttatggaat atggttacaa tattccggct acgtgcagaa
      241 aagtgcagga aagagtgaaa tcttccattg aaaatatgac gggactggaa gtgtccgatg
      301 tcaatatcag aattgcggga attgatatga caaagcagtc ataaaaggag cagcccagat
      361 gacaaggtca gagatacgtg aacatatttt caaacttctt ttcaggatag aattcaatgc
      421 ggcggaggat atgccgtcgc agatccggtt atattttgaa gatcctgtga acgcagaaga
      481 ggatgacagg aaaaaagccg gatggattca ggagaaggat gagcggtata tcagagaaaa
      541 gtatttgaag atcgcagata agattccgga actggacagt ctgatcgatc atgcggcaaa
      601 gggatggaag gcaagcaggc ttggaaaagt tgatttgacg atcctgcggc ttgctgtcta
      661 tgaagtgaaa tttgatgaag atattcctgt tggcgttgcg atcgatgaag ctgtggagct
      721 ggcaaaaaaa ttcggccagg atgaatcgcc ggcttttatt aacgggattc ttgctaaaat
      781 tgccatgtaa ggtctattat gaagagtgta tattctgtcg gacagattaa ttcgtatatt
      841 aagaacatgt tttcgcagga ctttatgctg cgttctattt atgtaaaggg agaggtatca
      901 aatgtaaaat accactcctc cggtcacata tattttacta tgaaggataa atcaggaaca
      961 attgcttgtg taatgtttgc aggctataga aagggtcttg attttacgtt aaaagaaggg
     1021 cagcaggtca tagccggagg aagtattgag gtctatgaaa gagacggcag atatcagctc
     1081 tatgcaaagg aactcatacg ggacggcgcg ggactgctct atgaacggtt tgaagaactg
     1141 aaaaaagaac tggaagaaat gggcatgttt tctgcgcaat ataagaaacc gattccaaag
     1201 tatgcaaaga aaatcggtat tgtgacagcg ccgacaggag ctgcggtccg tgatattatc
     1261 aacgtcgcta aaaggagaaa cccgtttgtt cagctgatcc tgtatccggc gcttgtgcag
     1321 ggggacggag caaaagaaag tatcgtgaat ggaatccatg cgctggaact ggccggtgtt
     1381 gatgttataa tcgcaggcag gggcggagga tccatcgaag atctgtgggc atttaatgaa
     1441 gagatcgtgg cacgggcgat ttttgagtgc agtataccga tcgtaagtgc ggtcgggcat
     1501 gaaaccgata cgacgatcgc ggattttgtt tcggatatgc gggcgccgac accgtcggcg
     1561 gcagcagagc tgacagtgtt ttcctatgag gattttaccc gccggtgttt ggacggccgg
     1621 gcgatgctgc ggcatggttt tgaccggcgg ctgaccgaac agaaaaaatt gtatgagcag
     1681 agacagctga ggctgtcgct tgtcagtccg cagagaagga tccatgagaa aaggatgcat
     1741 tgtgccggtc ttaccgaacg cctggagaaa aggatgcagg atcttcttat atacaaaaag
     1801 caccggctgc agctgcagat cgagagaatg aaagcacttt ctccgctgga gaggctgcag
     1861 aacggatatg cgt
//
LOCUS       DAOSYE010000478         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52223_length_1872_cov_3.577325, whole genome shotgun sequence.
ACCESSION   DAOSYE010000478 DAOSYE010000000
VERSION     DAOSYE010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52223_length_1872_cov_3.577325"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLU43_08315"
     CDS             complement(<1..461)
                     /locus_tag="PLU43_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28455.1"
                     /translation="MERGIEITLFSKFGIKMNGKQILESLSNTRKTKLFLSYLLLHKQ
                     KAVSHRELFELLWSGEDYSNPGTALRTLLYRYRALVDKAGVTQLKNSIISRRGAYQWN
                     QKLDISIDIFDFEDYSQIGLNKALSCDKRKECLCKAIDLYTGSLLPDSSSE"
     gene            638..>1872
                     /locus_tag="PLU43_08320"
     CDS             638..>1872
                     /locus_tag="PLU43_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28456.1"
                     /translation="MIHILIADKENSIASLIFESISEKKSELEICYAARNVDEVLHAL
                     ESYHPDLVLIDIAHFTSAELTQIRKAKDYAAPCRFIAASADSSAPQMQLAIRLGVDAF
                     LIKPVSSEILLKTISESEKKLEGEQFNRLSYRTYFQNRRSALLLDLLSYQKKFTYGEQ
                     EIYFPKSTERLFQILICEPYKKSRTEVIDKFSDQLKNLQKEQDYLCCEVEDNRQIILL
                     IGKAAHKTAHHFFQNHHEYEDKSTPYYSLFITCGRIVSSLDEVPLSYADALKLMEQRY
                     FLNEEIHVLTSDDLFIQTAEFQLENAEDYCEIFCSYLQSCSRVKLQDTLLKLQIKIRS
                     CTNSLPEIKLFLCDIFLSIKQTIMKKYPDAHIRFPSNYTIMYLIEHADYLEDVLDFYN
                     TQFESIMNSMGDSSGNNIL"
BASE COUNT          569 a          422 c          341 g          540 t
ORIGIN      
        1 tgctctgaac tggaatccgg aagcagactg cccgtatata gatcgattgc cttgcacagg
       61 cattcttttc tcttatcaca gctcaaagcc ttgttcaacc cgatctgtga ataatcctca
      121 aaatcaaaaa tatcaatgga aatgtccagt ttctgattcc actgatacgc tcccctgcgg
      181 ctgataatag aatttttcag ctgtgtaaca ccggccttat caaccaatgc acggtatcta
      241 tagagcagcg tacggagagc tgtccccgga tttgaataat cctcccccga ccataatagt
      301 tcaaacaatt ccctgtggga cactgccttt tgcttatgca gcagtaaata ggataaaaac
      361 agctttgtct ttctcgtatt gctcagactt tccaatatct gttttccatt cattttgatt
      421 ccaaatttgc tgaataaggt tatttcgatt ccccgttcca ttaatattcc tccgactctc
      481 cttgtttctg tcacatttgt caaacattat gattctatca caaggtgatt ataattacaa
      541 cctttttgca aagaaatctc gtacaaaaag cgggaacaga accttgacgg aataaacctg
      601 tttgctatat agtaaatgta atgcggaggc gtaatacatg atacatatat taattgcaga
      661 caaggaaaat tccatagcaa gtctgatctt tgaaagcata tccgaaaaga aatccgaact
      721 ggagatctgt tatgccgcaa gaaatgttga tgaagtgctt catgctttgg aaagctatca
      781 tcctgatctt gtattgatcg atattgcgca ttttacatct gcggaactta cgcagatccg
      841 gaaagcaaaa gattacgctg ctccctgtcg ttttattgcc gcaagtgcgg acagctccgc
      901 accgcagatg cagcttgcga tcagactcgg cgttgatgct tttttaatca aacccgtatc
      961 ttcggaaata cttctcaaaa ccatttccga aagtgaaaaa aaattggaag gcgaacagtt
     1021 taaccggctt tcctaccgga catactttca aaaccgccgt tccgcactgc tgctcgatct
     1081 tctttcatac caaaaaaaat ttacatacgg cgaacaggaa atttatttcc ccaaaagcac
     1141 agaacggctg tttcaaatat tgatctgtga accatataaa aaaagcagaa cagaagtcat
     1201 cgataaattc tcagaccagc tgaaaaacct gcagaaagaa caagattatc tatgctgtga
     1261 ggtggaagac aaccgccaga tcattctgct gatcggaaag gccgcacata agacagcaca
     1321 ccactttttc caaaaccacc acgaatatga ggacaaaagc acaccctatt actcattgtt
     1381 cattacatgc ggcaggatcg tttcttcttt ggatgaggtt cctctttcct atgctgatgc
     1441 tctaaagctt atggaacagc gctatttttt aaatgaggaa attcatgttc tgacatctga
     1501 cgacctgttt attcagacag ctgagtttca actggaaaac gcagaagact actgtgagat
     1561 cttttgcagc tatctgcagt cctgcagccg tgtaaaacta caggacacgc tcttaaagct
     1621 gcagatcaaa ataagaagct gtacaaactc tcttccggaa atcaaacttt ttctctgtga
     1681 catcttttta tcgatcaaac agacgatcat gaaaaaatat ccggatgcac atatccgttt
     1741 tccctctaat tatacgatta tgtatctgat cgaacacgca gactatctgg aagatgttct
     1801 ggatttctat aatacgcagt ttgagtcgat catgaattcc atgggcgatt cttccggaaa
     1861 taatatttta ta
//
LOCUS       DAOSYE010000479         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52265_length_1871_cov_3.169604, whole genome shotgun sequence.
ACCESSION   DAOSYE010000479 DAOSYE010000000
VERSION     DAOSYE010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52265_length_1871_cov_3.169604"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..358)
                     /locus_tag="PLU43_08325"
     CDS             complement(116..358)
                     /locus_tag="PLU43_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOO28457.1"
                     /translation="MKYLILGAGPAGLSFANRLLQNGETDFLVLEKESEAGGLCRSKY
                     VGGGPLDIGGGHFLDVRNQEVLDFLFPFMPKTEWCG"
     gene            complement(490..855)
                     /locus_tag="PLU43_08330"
     CDS             complement(490..855)
                     /locus_tag="PLU43_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO28458.1"
                     /translation="MEGKKTFGHVNMEKGLEMFDNDRDVYMEIVKLFCKNASKQIESI
                     VRSIEKEDYAAYGREIHTLKSLAGNLGAEELLRLAVKHDTAYKRKDYLFLKNSYWEIV
                     SLYSEVVQELNGFYADRDE"
     gene            complement(876..>1871)
                     /locus_tag="PLU43_08335"
     CDS             complement(876..>1871)
                     /locus_tag="PLU43_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28459.1"
                     /translation="DSYILPFTRYHSRYTQITEDDFQYDEYLESFKGFEPKWGSTGTT
                     EFVEPNIENVTEETAISEKAERYYRKYIELCMEENIPLLLTCAPYMIEEDAQGIYNYM
                     FSIAEEYGVPYLDFNKLYDELGLVFTEDMCEWSHLNEKGNVKYTTYLGNYIKENYEIP
                     DRRGDENYVTWQQDADRWTQELNLYNLPGIKDAEEFISLLDNPYYTVIVTINEGNDFS
                     VMSNEVETALAAIGISQETLAQKTAVVKDEQVLFQSSEEEYEWYTSLKKGDIIVNGIL
                     EENEENITAANRIYINGTEYSTTGSPLVITVYDNLLNTRIGTIGLIPEDGYSTIQ"
BASE COUNT          504 a          453 c          286 g          628 t
ORIGIN      
        1 aagtattcct taataactga atcggcattt aattcacctt ctttcagtat gttagctatg
       61 tgcatgctga tgttgggtac cgaggtggca aaaagttctg ccagttggtt ttggttcatc
      121 cacaccattc cgtctttggc ataaacggaa aaaggaaatc aagcacctcc tgatttctga
      181 catcaaggaa atgaccgccg ccgatatcca gcggacctcc tccaacatat ttgcttctgc
      241 acagtccgcc ggcttccgat tctttttcca aaacgagaaa atctgtctcc ccattctgca
      301 gtaaacggtt cgcaaacgat aatcctgccg gccctgctcc aagtattaaa tatttcatat
      361 ataagtcttc cgttccgatc caataatctg taatacttcg ctcttattct atcagttttc
      421 caaaaaaaca tcaagcaaat agaaaatgac cctcctgcat tcaaatccgg gcgggtcata
      481 cgctgcattc tattcgtctc tgtctgcata aaagccgttc agctcctgca ctacttcgct
      541 atataaagat acgatttccc aataggaatt ctttaaaaaa aggtaatcct ttctcttata
      601 cgcggtatca tgtttgacag ccagccgcaa aagctcttcg gcccccaaat tgccggcaag
      661 acttttcagt gtatgtattt ctcttccata agctgcataa tcttcttttt caatgcttct
      721 tacaatactc tctatctgtt ttgacgcatt tttacagaat aatttgacga tttccatata
      781 aacatcccgg tcattatcaa acatttccaa ccctttttcc atatttacat gtccaaatgt
      841 ttttttacct tccattttat tctaccatcc gtccatcatt gtattgtaga atatccatct
      901 tccggaatta atccaattgt accgattcgg gtattcagta aattgtcata aacagtaatt
      961 accaacggac tgcctgttgt actgtactct gtaccattta tatatattct gtttgctgct
     1021 gttatatttt cttcattctc ctccaatatt ccgtttacta ttatatcacc tttttttaaa
     1081 gaagtatacc attcatattc ttcttccgat gactgaaaaa gaacctgctc gtcctttacg
     1141 actgcggttt tttgcgcaag tgtctcctga ctgattccga tcgctgccag cgctgtctct
     1201 acttcattcg acatgacaga aaaatcattt ccctcattaa tcgttacgat gaccgtataa
     1261 taagggttat caagcaggga aataaactcc tctgcatcct tgataccggg caggttatat
     1321 aaattcagct cctgtgtcca tctgtccgca tcctgctgcc acgtaacgta attctcatct
     1381 ccccgtctgt ccggaatttc gtaattctct tttatataat ttccaagata cgtcgtatat
     1441 tttacattgc ctttttcatt caaatgagac cactcacaca tatcttcagt aaacacaaga
     1501 cctaattcat catacagttt attaaaatca agataaggaa ctccgtattc ttctgcgatc
     1561 gaaaacatat aattataaat tccctgtgca tcctcctcga tcatataggg cgcacatgta
     1621 agcagcagcg gtatgttttc ttccatgcaa agttcaatat attttctgta atagcgctcc
     1681 gctttttcgg agatcgcagt ttcttccgta acattttcga tattgggttc aacaaattct
     1741 gtcgtacccg tggaacccca tttcggctca aaccctttaa atgattccag atattcgtca
     1801 tattgaaaat cgtcttccgt aatctgcgta tatctggaat gatatctggt aaatggaagg
     1861 atataactgt c
//
LOCUS       DAOSYE010000480         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52364_length_1869_cov_3.101433, whole genome shotgun sequence.
ACCESSION   DAOSYE010000480 DAOSYE010000000
VERSION     DAOSYE010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52364_length_1869_cov_3.101433"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..533
                     /locus_tag="PLU43_08340"
     CDS             51..533
                     /locus_tag="PLU43_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO28460.1"
                     /translation="MREKILTFIEKNSRIELKELAILLGMSEVDVANELEAMEKEGII
                     CGYHTLIDWDKVTSERINALIEVRVTPQRGHGFDSIAERIYKYPEVEATYLISGGYDL
                     LVSLEGKTLREVSRFVSEKLSTLESVISTSTHFVLKKYKDHGTILHEKYDDERMIVTP
                     "
     gene            530..1702
                     /locus_tag="PLU43_08345"
     CDS             530..1702
                     /locus_tag="PLU43_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO28461.1"
                     /translation="MRNPLSDTISAIKPSGIRKFFDIASERKNTISLGVGEPDFDTPW
                     HIRDEGIYSLEKGRTFYTSNCGLIELRTEICNYLKRRICVAYDPKNEVIVTVGGSEAI
                     DIGLRAMINPGDEVLIPQPSYVSYEPCAVLAGAKPVIIELKNENEFRLTPKQLLEAIT
                     SKTKVLILPYPNNPTGAVMEKEDLEKIAEICIEKDIFVMSDEIYSELTYCGDHVSIAS
                     LPGMQERTILINGFSKAYAMTGWRLGYACGPKEIIAQMTKIHQFAIMCAPTTSQYAAI
                     DALKNGDTDVAVMRESYNQRRNYLMHAFGEMKLPCFEPYGAFYVFPCIREFGMSSEEF
                     AFALLEAENLAVVPGSAFGDSGEGFLRISYAYSLENLKIAIGRITRFVEKLRNEKK"
     gene            1699..>1869
                     /locus_tag="PLU43_08350"
     CDS             1699..>1869
                     /locus_tag="PLU43_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOO28462.1"
                     /translation="MNIVLLEPEIPSNTGNIGRTCVATGTRLHLIEPLGFRLNEKSIR
                     RAGMDYWEHLDVT"
BASE COUNT          548 a          348 c          504 g          469 t
ORIGIN      
        1 aaaacagatg aactgtacaa gatctatgaa taaagaatcg ggagaaaaat atgagagaaa
       61 aaatattaac atttattgaa aaaaacagca ggatagaatt aaaagagctt gccattcttc
      121 ttggcatgtc cgaggtggat gttgcaaatg aactggaagc catggaaaag gaagggatca
      181 tctgcgggta tcatacgctg atcgactggg ataaagtaac ttcggagcgg atcaacgcat
      241 tgatcgaggt gcgtgtaaca ccgcagaggg gccatggatt tgattccatt gcggagcgta
      301 tttataaata tccggaggtg gaggccactt atctgatctc gggagggtat gatctgcttg
      361 tgtcactgga aggaaaaacg ctgcgggagg tttcccgttt tgtttccgag aagctttcca
      421 cactggagtc cgtgatcagt acatccacac attttgtttt aaagaaatat aaggatcatg
      481 gaacgatcct gcatgaaaaa tatgacgacg aaaggatgat agtgacgcca tgagaaatcc
      541 gctgtccgat acgatctctg ctatcaagcc gtccgggatc cgcaagtttt tcgatattgc
      601 cagtgaaaga aaaaatacga tttccttagg agtgggcgaa cctgactttg atacgccgtg
      661 gcatatccgt gatgaaggaa tctattcgct ggaaaaagga agaacgtttt atacatccaa
      721 ctgcggtctg atagaactga gaacggagat ctgtaattat ttaaagagaa gaatttgtgt
      781 tgcatatgat cctaaaaacg aggttatcgt aacggtaggc ggcagcgaag cgatcgatat
      841 cggattgcgc gcgatgatca atccgggaga tgaagtattg atcccgcagc cgagttatgt
      901 gtcttatgag ccttgtgcag tgcttgcggg agcaaaaccg gtcattatcg aactgaaaaa
      961 cgaaaatgaa ttccggctga cgccaaagca gctgctggag gcaattacgt caaaaacgaa
     1021 agtgctgatc ctgccgtatc cgaataatcc gacaggtgcg gtcatggaaa aagaggatct
     1081 tgagaagatc gcagagatct gcattgaaaa ggatattttt gtgatgtcgg atgagatcta
     1141 ttccgaactg acttactgcg gagatcatgt ttcgatcgca tcacttccgg ggatgcagga
     1201 gaggaccata ctgatcaatg gattttcaaa ggcttatgca atgacaggct ggagacttgg
     1261 ttatgcgtgc ggtcccaaag agatcattgc ccagatgacg aagatccatc agtttgcgat
     1321 catgtgtgcg cctacaacga gccagtatgc tgcgatcgac gcattgaaaa acggagatac
     1381 cgacgttgct gttatgcggg aatcctataa tcagcgcaga aattatctga tgcatgcgtt
     1441 cggggaaatg aagcttccgt gctttgaacc atacggtgct ttttatgtat ttccctgtat
     1501 cagggagttt gggatgtctt ccgaggaatt tgcatttgcg ctgcttgaag cggagaatct
     1561 ggcagttgtg ccgggaagtg cgtttggaga cagcggagag ggatttttgc gtatttccta
     1621 tgcgtattcc ctggaaaatt taaagatcgc aatcggaagg atcacaagat ttgttgaaaa
     1681 gctgaggaat gagaaaaaat gaacatcgta ctgcttgagc ctgaaattcc atccaatacc
     1741 ggaaatatcg gaaggacctg tgtggcaacg ggaacgagac tgcacctgat cgagccgctt
     1801 ggttttcggt tgaatgaaaa atcgattcgg cgggccggta tggattactg ggagcatctt
     1861 gatgtaacc
//
LOCUS       DAOSYE010000481         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52452_length_1867_cov_3.613687, whole genome shotgun sequence.
ACCESSION   DAOSYE010000481 DAOSYE010000000
VERSION     DAOSYE010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52452_length_1867_cov_3.613687"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..>1867)
                     /locus_tag="PLU43_08355"
     CDS             complement(424..>1867)
                     /locus_tag="PLU43_08355"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOO28463.1"
                     /translation="LKLYHAAICLTIFMVYSLYLLLTKNAYAGKTQIIVSGAVFMLTL
                     VGVYGFHYKKEYKIPIIAGFFMLQAGDLLCNGYLSIETYFPDRETDESCSIEAFSDFI
                     DETTTIVDQIEAQDSDFYRIDKMYRRTHNDAMMIGYNGLSHFSSCETEQVKQFMGNMG
                     FRDNGNWAFFGEGSTSFADCFMGLKYMISQYDETAKPYQKLMTYQDKYVYQNPFALSL
                     AFGTTDAITEVSADTGNHFEYQNALAESFSGDPYDIYRPVLLKDIKLVNVEEQDHIYK
                     KIDQEQEGYIEYQFSADSSDFIYMYFDAPSIQDTTIVINDLEKASYFSRYGWSIREVG
                     HFNPGELVRIRVYLNQEQIEIDNYEFYYEDETELANWYQDAEKTTCQIQKNSSSHLIG
                     TINAAEGTDLIVFSIPYDTNWNVKIDGNTVKTKEVLDCLMAADITSGTHTIEMRYIPS
                     GFLRGLPVSLLSFLILVVIVCYEKRKKKAS"
BASE COUNT          558 a          410 c          294 g          605 t
ORIGIN      
        1 ttttttgaat tttcagggtt ggttatactg ttcagttgtc aaggtgcttt tgtttctgtc
       61 tgtctggccc ggatatcgtc atctgtatct gtcattatac atatgagaga tactgccgta
      121 taccctgcca cacacttgct gccggctgct ttctgtaacc cgcaactttt gtataatacc
      181 atgcaaaaaa catgaagtca ataccatttt ccaaaatatt ttttttaaat taccctgccc
      241 ctatatctac tcttatagat aatcggaact actctatttt gtggtaataa aagactaaaa
      301 aaatagactt cccagataat tgatattgac acaaaagaat ccacccttaa ttcgggtgga
      361 cttttattat accattgaaa acagttactt gtcaacgaat tcgccgaatt attcaacaaa
      421 aaattaactt gcttttttct ttcttttttc ataacagacg attacgacta aaatcaaaaa
      481 ggaaagcaga gaaacaggaa gaccccggag aaaaccgctt ggaatatacc gcatttcaat
      541 tgtatgcgta cctgacgtaa tatctgccgc catcagacag tccagtactt cttttgtctt
      601 gaccgtgttg ccgtcgattt taacattcca gtttgtatca tagggaattg aaaaaacgat
      661 cagatccgtt ccctccgcag catttattgt tccaatcaaa tgggaactgc tatttttctg
      721 tatctggcat gttgtttttt cagcatcctg ataccaattc gctaattccg tttcatcttc
      781 gtaataaaat tcatagttat cgatctctat ctgttcctga ttcaaataaa ctcttatacg
      841 gacaagttct cccggattaa aatgccccac ttctctgatg gaccatccat atctggaaaa
      901 ataggacgcc ttttctaaat cattaataac gatcgttgtg tcctgtatac ttggcgcatc
      961 aaaatacata taaataaaat cagaactgtc tgccgaaaac tgatattcaa tatatccttc
     1021 ctgttcctgg tcaatttttt tataaatatg atcctgttcc tctacattga ctaattttat
     1081 gtcctttaac agaaccggcc tgtagatgtc atacggatcg ccggaaaaac tttccgcaag
     1141 tgcgttttga tattcaaaat gatttcccgt atctgccgac acctccgtaa ttgcgtctgt
     1201 tgttccaaat gccagactta aagcaaacgg attctgataa acatatttgt cctggtatgt
     1261 catgagcttc tgataaggct tcgccgtctc atcatactgg gagatcatat atttcaagcc
     1321 cataaaacaa tcggcaaaag aagtgctccc ttctccaaaa aacgcccagt ttccattatc
     1381 acgaaagccc atatttccca tgaattgttt tacctgctct gtttcacagg aactgaaatg
     1441 cgataatccg ttatagccga tcatcatggc atcattatgt gttcttctgt acattttatc
     1501 tattctgtaa aaatccgaat cctgtgcctc aatctgatca acgatcgttg ttgtttcatc
     1561 tataaaatct gaaaatgctt ctatactgca tgattcatcg gtttccctgt ccggaaaata
     1621 agtttcgatc gacagatatc cattacaaag cagatctcct gcctgcagca taaaaaatcc
     1681 ggcaataatt ggtattttat attctttttt ataatgaaat ccataaacac caaccagcgt
     1741 cagcataaaa accgcaccgg acacaataat ctgcgttttg cctgcgtaag cattcttcgt
     1801 cagcagtaaa taaagagaat agaccataaa tatcgtaaga cagatggcgg catgatataa
     1861 tttcaat
//
LOCUS       DAOSYE010000482         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52467_length_1867_cov_2.437086, whole genome shotgun sequence.
ACCESSION   DAOSYE010000482 DAOSYE010000000
VERSION     DAOSYE010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52467_length_1867_cov_2.437086"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLU43_08360"
     CDS             <1..835
                     /locus_tag="PLU43_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28464.1"
                     /translation="NDFWSSVLAGAEMAAKENNVRLEINSPTDEMHIDEQNQLIYEAI
                     EERPDVIAVSPSSKNENNKALLAVKTAGIKLIYIDSKTDEEIQDVCVSTDNVAAGKKM
                     AEPMLGTIDQTSKIAIVSHVRNSSTAEEREIGFREGLGSYESQIVDVVYSNSMKDIGY
                     QVTKELLTKEPDISYLACLNEDSAVGAAGAVKELGLVGKICIVGFDNSTEEILSLEEG
                     VFSAIVVQKAFSMGYLGIESAAELARGEKVETYIDSGSVLITKENMYKEENQELLFPF
                     Y"
     gene            1082..>1867
                     /locus_tag="PLU43_08365"
     CDS             1082..>1867
                     /locus_tag="PLU43_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28465.1"
                     /translation="MEKIKNNPIVRKIGFNRIILCCVLILMYILFAILSPTFLSYSRI
                     LSALNYAYFLGFLALGVTFVIGTGGIDFSIGPVMFCAALISGQMLTKSGVPLPICLLV
                     SIIVGFIFGLMNGWLVAYRGLPSFITSMAIMMLAKGVGSVFTKIQSVSWPQSSQVGGW
                     YRNLVKVRVGTTPLPTGLLILLIAAIICSIVLNKTRVGRYILCIGDNKEAVRLSGVNV
                     KKWEMIAYVICGTLAGVAAIFYVGAYTTVQPGLGDTFNNEAIAS"
BASE COUNT          534 a          333 c          464 g          536 t
ORIGIN      
        1 taatgatttc tggtcgtccg tactggcagg ggctgaaatg gcggccaaag aaaacaatgt
       61 cagacttgag atcaattctc cgacagatga aatgcatatt gatgagcaga atcagctgat
      121 ttatgaagcg atagaagaac gcccggatgt gatcgcggta tccccttctt ctaaaaatga
      181 aaataataag gcgctgctgg cagtgaaaac cgccggaatc aagctgatct atattgattc
      241 caagacagat gaagagattc aggatgtctg tgtttcgaca gataatgttg cggccggaaa
      301 aaaaatggcg gaaccgatgc ttggcacgat cgatcaaaca tcaaaaattg cgatcgtatc
      361 ccatgtgaga aattcttcca cggcagaaga acgtgaaatc ggttttcgag agggactcgg
      421 ttcctatgaa tcacagatcg tagatgttgt ttacagtaat tccatgaaag atatcggata
      481 ccaggtaaca aaagagcttt taacaaaaga gccggatatc agttatctgg catgcctgaa
      541 tgaagactcg gcagtcggtg cggcgggagc tgtcaaagag cttggactgg tcgggaagat
      601 ctgtatcgtt ggctttgata attccacgga agaaatattg agcctggaag agggcgtgtt
      661 cagtgcgatc gttgtacaga aggcgttcag tatggggtat cttgggatcg aaagcgcagc
      721 agagcttgca aggggtgaaa aagtagagac gtatattgat tccggatccg tactgattac
      781 aaaagaaaat atgtataaag aagaaaatca ggaattattg tttccttttt attaacatct
      841 gatgaaagat tccttatacg gagccatagg acggcttgtg taaaaaccac agaaaaacga
      901 tgtaatactg tcgtattctg tggttttttc gttccgcttt tgctttttta ttgttttatg
      961 ccttttaaag taaaattttc aacattctgt ggaagatatt ctattaaatt attcattttt
     1021 atgtcatata atattcacat attagccgaa tggcggccaa aaaatatagg aggaagaaaa
     1081 agtggagaag attaaaaaca acccaattgt tagaaaaata ggatttaaca gaatcatact
     1141 ttgctgtgta cttattttga tgtatatcct gtttgcgatt ctaagtccta cgtttttaag
     1201 ttattcaaga atactgagcg ctttgaatta tgcatatttt ctgggatttc tggcacttgg
     1261 tgttactttt gtaataggga ccggaggcat tgatttttcg atcggaccgg taatgttctg
     1321 tgcggctttg atttcaggac agatgctgac aaaatcagga gtgccgcttc cgatctgttt
     1381 gcttgtcagt atcattgtag ggttcatttt cggcctgatg aacggatggc tcgttgctta
     1441 ccgcggtctg ccgtcgttca ttacttccat ggcgattatg atgttggcaa agggcgttgg
     1501 ttccgtcttt acaaaaatcc agtcggtcag ctggcctcag tcctcacagg tgggcggctg
     1561 gtacaggaac cttgtaaagg tcagagtcgg aacaacgcct cttccgacag gactgttgat
     1621 tttgctgata gcagcgatca tttgctcgat cgtactgaac aagacaagag tcggacgcta
     1681 tattttatgt attggtgaca ataaagaagc agtcaggctt tccggtgtga atgtaaagaa
     1741 gtgggaaatg attgcctatg ttatctgcgg aacacttgcc ggtgttgcag cgatcttcta
     1801 tgtaggggct tatacgaccg tacagccggg actcggcgat acgttcaaca atgaagcgat
     1861 cgcaagc
//
LOCUS       DAOSYE010000483         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52582_length_1864_cov_3.382532, whole genome shotgun sequence.
ACCESSION   DAOSYE010000483 DAOSYE010000000
VERSION     DAOSYE010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52582_length_1864_cov_3.382532"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..682)
                     /locus_tag="PLU43_08370"
     CDS             complement(170..682)
                     /locus_tag="PLU43_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28466.1"
                     /translation="MKKKFSSGFNIGSSSILVTFVLLCLVTFAALSFLSANSDYRLSR
                     QTAERTTAFYEANQMAEIYMANIEALLAKQNSAAADEVSYMAAIPALFSDNDHIAVDA
                     SSSPVTLSYSIAVNKTQDLHVCLSVHYPDEQDPGLFHIQTWQTVTNETWQEEADDAGS
                     LNEGSGLIEF"
     gene            complement(710..1150)
                     /locus_tag="PLU43_08375"
     CDS             complement(710..1150)
                     /locus_tag="PLU43_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28467.1"
                     /translation="MNKQPKSKTSLFLMELIITILFFSIAGAVCMQLFVKAHTISEKT
                     KELNHAVTAAQGFAEVMRGTDGSIESILAVYPDAIQGSDTFFEVFYDQDFQPCEPENA
                     VYVSDITLLPNGAIQNMEIRIVKLSDYEEIYSLNATKYMNIPKG"
     gene            complement(1143..1652)
                     /locus_tag="PLU43_08380"
     CDS             complement(1143..1652)
                     /locus_tag="PLU43_08380"
                     /inference="COORDINATES: protein motif:HMM:NF027478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HOO28468.1"
                     /translation="MNFSKREKSIVDILFLLALFCVFLVSALFIVLFGAKIYRSTVAD
                     MNTNFTSRTALSYITEKMRQHDSAGGAQVTFIDGQPVLCLNQENDGVKYCTYLFSNDG
                     YLKEVTAKENYDFDLSSGQNIIELSEFTAEEIQDSLYKFHITDIDGNQIDFYVSLYSH
                     TNEGETPDE"
     gene            complement(1649..>1864)
                     /locus_tag="PLU43_08385"
     CDS             complement(1649..>1864)
                     /locus_tag="PLU43_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28469.1"
                     /translation="SSVNDEKEILTNAVNRDIVHCYAVEGMYPPSLHYIEKNYGLTYD
                     HDRFIIDYEAIGSNIMPSVMIIERNPE"
BASE COUNT          535 a          383 c          390 g          556 t
ORIGIN      
        1 gctgtttcct gcggcatcat tttcttttca ttaaataaat gcatttgacc gttcgttttg
       61 taggtcagcg gaagtccggc tacgatgaaa atatccgaag caccgtcctg agtaattttt
      121 gataaagtat caacgattgg cataagcaaa attcctccct gcagattaat taaaattcaa
      181 taagtcctga gccttcgttt aaagagccgg catcatcagc ttcttcctgc catgtttcat
      241 ttgtgactgt ctgccatgtc tgaatatgga acagcccagg atcctgctcg tcaggataat
      301 ggaccgacag acacacgtgc aggtcctgtg ttttattgac agcaatggaa tagctcaaag
      361 taactggtga tgaagaagcg tccactgcaa tgtgatcgtt gtcggaaaac aaggctggaa
      421 tcgcggccat ataggagact tcgtccgcag cagcgctgtt ttgcttggcc agcagcgctt
      481 caatattggc catatagatt tctgccatct ggtttgcttc atagaaagca gttgttcgct
      541 ccgcagtctg cctgcttagt ctgtaatcgg aattggcgct taaaaaagaa agcgcagcaa
      601 acgttacaag acagagtaat acaaatgtaa caagtatgga ggaagaacca atattgaatc
      661 cggaagaaaa cttttttttc ataataaaaa ctcctttggc agaccggtat taaccttttg
      721 gtatattcat atattttgtg gcgttcaggg aatagatctc ttcataatca gacagcttta
      781 caatacggat ttccatattt tgaattgctc cgtttggaag cagtgttata tcactgacat
      841 aaacagcatt ttccggttcg cacggctgaa aatcctgatc ataaaatact tcgaaaaagg
      901 tatcgctgcc ctgtatggca tccggataga cagccaggat gctttcgatc gatccgtccg
      961 tgccgcgcat aacttccgca aacccctggg ctgcggtcac agcatgattt aattcctttg
     1021 ttttttcgct gatcgtatgc gctttgacaa atagctgcat gcatacggct cctgcaatcg
     1081 agaaaaacaa aatagtaatg atcagctcca tcagaaacaa agaggtttta gattttggct
     1141 gtttattcat cgggtgtttc tccttcgttt gtatggctgt ataaagaaac ataaaaatca
     1201 atttgattgc cgtctatatc cgtaatatga aatttataaa gggaatcctg gatctcttcg
     1261 gctgtaaatt ccgatagttc aataatattc tgtccgctgg acaaatcaaa atcataattt
     1321 tccttggcgg ttacttcctt taaataaccg tcgttagaga atagataagt acagtatttt
     1381 acaccgtcgt tttcctgatt cagacacagt accggctgtc cgtcaataaa agtgacctgg
     1441 gcgcctcctg cggaatcatg ctgccgcatt ttttccgtga tgtaggatag ggcggtacgg
     1501 gaagtaaaat ttgtattcat atcggcaact gttgaacggt atatttttgc gccaaacaat
     1561 acgatgaaaa gtgctgatac aagaaagacg caaaaaagag ccagcaggaa cagaatatca
     1621 acaatggatt tttctctttt actgaaattc attctggatt cctttctatg atcattacgg
     1681 atggcatgat attagagccg atcgcttcat aatcaataat aaagcggtca tgatcgtaag
     1741 taagaccgta atttttttcg atataatgta aggaaggcgg atacatgcct tccacagcat
     1801 agcagtgtac aatgtctctg ttaacagcgt tggtcaggat ctctttttca tcattgaccg
     1861 aact
//
LOCUS       DAOSYE010000484         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52587_length_1864_cov_3.095633, whole genome shotgun sequence.
ACCESSION   DAOSYE010000484 DAOSYE010000000
VERSION     DAOSYE010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52587_length_1864_cov_3.095633"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1090
                     /locus_tag="PLU43_08390"
     CDS             233..1090
                     /locus_tag="PLU43_08390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO28470.1"
                     /translation="MKLSKEELLTFISVQKKIFDIVRLVDVSMTIQYTVLEDGTIEQQ
                     PHECYAVWNKNSRCENCISAKALALKSKLTKFEFVNDEIYFVISIYTEVDDTVYMIEM
                     VSHLDDETLFGAYRKNEFIQTIETYNKKLYRDVLTGAYNRRYYHEQLRQLYDINSIVM
                     LDADDFKNINDTYGHSIGDFVLKEIVRISSSCIRSCDYIVRLGGDEFLLLFRNISLEI
                     LNDNLKTINRTISSIHADKHPELRVSASIGAVYSTGPAADLIDYADNALYEAKRRKNC
                     IVIKDLSSQ"
     gene            complement(1112..>1864)
                     /locus_tag="PLU43_08395"
     CDS             complement(1112..>1864)
                     /locus_tag="PLU43_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOO28471.1"
                     /translation="QNEPAAEQEWDSCLYTGEEEGIFAVCHLRKELDKAGLTDVKILI
                     WDHNKDQIIERCEQSFAVEGAKTAIAGIAYHWYSGDHFEALQYVSRKYPDKEIIFTEG
                     CVEYSRFAASDQTENAEMYAHNIIGDLNAGMSGFLDWNLLLDHQGGPNHVYNFCDAPI
                     MCNCDADTIDVKLSYYYIGHFSRFIKPGAVRLLTSSYNKALECAAFENPDGERLAVIL
                     NAGEEEQVFSLYMEGKATKMYLEGHSIMTAVI"
BASE COUNT          570 a          414 c          341 g          539 t
ORIGIN      
        1 tggcaggata ttaatgtatg atgaaagttc tgccaacgat tagttgacag aaacgaaaca
       61 gggatgaata ataaaacctg ttgagagacg tctgtctcta tggtagaatt atattaagaa
      121 ataaaactac acttttatac acaggcacct catatgattt aacgactata ttacacttca
      181 caattttacc aaattatatg tatccatggg ccagaataag gaggattttt gtgtgaagtt
      241 atctaaggag gagctgctca cttttattag cgtacaaaaa aaaatatttg acatcgtacg
      301 tcttgtagac gtatcaatga caatacaata tacagttttg gaggacggga ccatcgagca
      361 gcagccgcat gagtgctatg cggtctggaa taaaaacagc cggtgtgaaa actgtatttc
      421 agcaaaagcg cttgccctga agtccaaact gacaaaattt gaatttgtca acgatgaaat
      481 ttactttgta atatcaattt atacggaagt agacgatact gtctatatga tcgaaatggt
      541 atcccatctg gacgatgaga ctctttttgg tgcttatagg aaaaatgaat ttatccaaac
      601 gatcgaaaca tataataaaa aactgtaccg ggatgtatta acaggcgcat acaaccgccg
      661 gtattaccat gaacagttaa gacagttata tgacatcaat tctatcgtca tgctggatgc
      721 cgatgatttt aaaaatatca atgacactta cggacattcc atcggtgatt ttgtgctgaa
      781 agaaatcgtc aggatcagtt cttcttgtat caggtcctgt gattacattg tccggctcgg
      841 cggcgatgaa tttcttctgc tttttcgcaa catctcctta gagatactca atgacaattt
      901 aaaaacaatc aatcggacga tttcatccat acacgcggac aagcacccgg aacttagagt
      961 ttcagccagt atcggagccg tatattccac cggacctgcc gccgatctga tcgattatgc
     1021 cgacaatgct ctatatgaag ctaaacggcg aaaaaactgt attgtaataa aagacctgtc
     1081 atcccaataa tttttataaa tttacagtct cctatatgac agccgtcatg atcgaatgtc
     1141 cctccagata cattttggtt gccttccctt ccatatacag agaaaatacc tgttcttcct
     1201 cccctgcatt taaaataacg gcaagccttt caccgtccgg attttcaaat gctgcacatt
     1261 ccagcgcctt attataggaa gaagtcagca gccttactgc tcctggtttt ataaatctgc
     1321 tgaaatgccc aatataataa taagaaagct tcacatcgat cgtatctgca tcacagttgc
     1381 acatgatcgg cgcatcacag aaattatata catgattggg tccgccctga tgatcgagca
     1441 aaagattcca gtcaagaaat cctgacattc ctgcgttcag atccccgata atattatgcg
     1501 catacatttc cgcattttcc gtctgatcag atgctgcaaa tctggaatat tcaacacacc
     1561 cctccgtaaa aattatttct ttatccgggt attttcggct gacatactga agcgcttcaa
     1621 aatgatctcc cgaataccaa tggtaagcga tcccagctat agccgtcttc gctccctcta
     1681 ccgcaaacga ctgttcacag cgttctatga tttgatcctt attatgatcc cagatcaata
     1741 ttttcacatc cgtcagaccg gctttgtcaa gttcttttct cagatggcaa acagcaaaaa
     1801 tgccttcctc ttctcccgta tacagacagg aatcccattc ctgttccgca gcaggttcgt
     1861 tctg
//
LOCUS       DAOSYE010000485         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52690_length_1862_cov_3.025457, whole genome shotgun sequence.
ACCESSION   DAOSYE010000485 DAOSYE010000000
VERSION     DAOSYE010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52690_length_1862_cov_3.025457"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PLU43_08400"
     CDS             complement(<1..151)
                     /locus_tag="PLU43_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO28472.1"
                     /translation="MSREEIFERLNYVFRDVFDDDSIAVTDETTAADIRGWDSLIHIT
                     LMDAVE"
     gene            complement(129..>1862)
                     /locus_tag="PLU43_08405"
     CDS             complement(129..>1862)
                     /locus_tag="PLU43_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HOO28473.1"
                     /translation="LLASGAAFLEKKIAVLGGSTTHDIAAVMDLFLLNQGIKAEFYEC
                     EYAKYWEDLMFDNPELMEFAPDVIYIHTSNRNIRQFPHMDQSEAEIDAMLDTDFMHFQ
                     KMWEKAETVYHCPIIQNNFEYPFYRLLGNADATDIHGRVCYINRLNERFAAYAREHKD
                     FFIQDINYLSAAYGLDKWADPLYWHMYKYALSMEAIPTLAYNVGNMIKSIYGKNKKAL
                     VLDLDNTLWRGIVGDDGPENLEIGPETSIGQVYSEFQNYIKEQKEIGVILNICSKNDY
                     ENAVAGLKHPDSSLSPEDFILIKANWEPKSKNIIETAKELNLLPESFVFVDDNPAERE
                     IVAAQVPQVTTPDIGSAENYIQVLDHAGYFEVTNLSADDAARNQMYKQNTERLKLEQS
                     FSDYGEYLRSLEMKGTIRNFTQVYMERIAQLTNKSNQFNLTTKRYTRGEIENAAESED
                     YIDLYGKLEDKFGDNGVVSVVIGRKAGDILHVELWIMSCRVLKRDMEFAMMDELVAAG
                     RQAGISIIRGYYYPTAKNKMVREFYKQMGFTKTAEDEEGNTTWEFPVTDSYQNQNKWI
                     KVNENEQRRNI"
BASE COUNT          455 a          499 c          345 g          563 t
ORIGIN      
        1 cttcaaccgc atccatcagg gtaatatgaa tgaggctgtc ccatccgcgg atatctgctg
       61 ccgtcgtttc atccgtaacc gcaatgctgt catcgtcaaa tacatcacgg aatacatagt
      121 tcaaacgctc aaatatttct tctctgctca ttttcattca cctttatcca tttattttga
      181 ttttgataag aatccgttac gggaaattcc catgtcgtat ttccttcttc atcctctgcc
      241 gtctttgtaa agcccatctg tttataaaac tcccgaacca ttttattttt ggcagtcggg
      301 taataatatc ctctgattat ggaaatgccc gcctgcctgc cggcagcaac aagttcgtcc
      361 atcatggcaa attccatatc ccgctttaat acgcggcagc tcatgatcca cagctccaca
      421 tgcaggatat ctccggcttt ccgtccgatc acaacagata cgactccgtt gtctccgaat
      481 ttgtcttcca gcttcccgta taggtcaata tagtcttccg attctgcagc gttttcgatc
      541 tcgcctcttg tatatcgttt tgttgtcagg ttgaactgat tgctcttatt tgtaagctgg
      601 gcaatccgtt ccatatagac ctgtgtgaaa ttcctgatcg tgcctttcat ctcgagcgat
      661 cgtaaatatt ccccgtaatc cgaaaaactc tgctcaagct ttaaccgttc cgtgttttgt
      721 ttgtacattt ggttccttgc cgcgtcatcc gcggacagat tcgtcacttc aaaatatccg
      781 gcatggtcaa gcacctggat ataattttcc gcgctgccga tatcaggcgt ggtcacctgc
      841 ggcacctgcg cggcaacgat ctcccgctcc gcaggattat catcgacaaa cacaaacgac
      901 tccggcagca gattcagctc ttttgccgtc tctatgatat ttttgctttt cggctcccag
      961 ttggccttga tcagtataaa gtcctccggt gacagggaac tgtccggatg cttcagccct
     1021 gcaaccgcat tttcgtaatc gttcttggaa cagatgttca aaataacacc aatttccttc
     1081 tgttccttga tataattttg aaattcactg tatacctgtc cgatagatgt ttccggtccg
     1141 atttcaaggt tttccggacc atcgtccccg acaatgccgc gccatagcgt attgtcaaga
     1201 tccaggacca gtgccttttt attttttcca taaatagact tgatcatatt gccgacatta
     1261 taggcaagtg tcgggatcgc ctccatactg agcgcatact tatacatatg ccagtaaagc
     1321 ggatccgccc acttatccaa accgtaagca gccgacagat agttgatgtc ctgaataaaa
     1381 aagtccttat gttcccgcgc atatgcggca aaccgctcat tcagcctgtt aatatagcag
     1441 actctgccat gaatatccgt agcgtccgca ttcccaagca gcctgtagaa cggatattca
     1501 aaattattct gaatgatcgg acaatgatag accgtctctg ccttctccca catcttctga
     1561 aaatgcataa agtcagtatc cagcattgca tcgatctctg cctccgattg atccatatgc
     1621 ggaaactgtc tgatatttcg attactggtg tggatataaa taacatccgg cgcaaattcc
     1681 ataagctccg gattgtcaaa catgagatct tcccaatact ttgcatattc acattcgtaa
     1741 aactctgcct ttatcccctg attcagtaaa aacagatcca tgaccgctgc aatatcatgt
     1801 gtggtcgacc ctcctaaaac cgcaattttt ttttccagaa acgctgctcc tgatgccagc
     1861 ag
//
LOCUS       DAOSYE010000486         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52750_length_1861_cov_2.208749, whole genome shotgun sequence.
ACCESSION   DAOSYE010000486 DAOSYE010000000
VERSION     DAOSYE010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52750_length_1861_cov_2.208749"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLU43_08410"
     CDS             <1..955
                     /locus_tag="PLU43_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO28474.1"
                     /translation="GGDLLDRVVMIRRQVALELGTVVPIIRLRDNIQLNPNQYIIKIK
                     GIQVSEGEILFDHYMAMNPGFVEEEITGIPTFEPSFHLPAIWITESQRERAESMGYTV
                     VDPPSIIATHLTEVIRQHISELLSRQDVQNLVNNIKESNPSLVEELTPKLLGLGEIQK
                     VLQNLLREGVSIRDLLTIFETLADHAAVTRDANVLTEYVRQSLKRAISAKYFPANEST
                     SVVTLDPKIEQEIMGSVKQTEQGAYLTLDPERTKTIIKAVEKEITKLENLGKNPIIIT
                     SPIVRMYFKKMTEDYFKDLIVVSYNDIDSNVELQSVGMVAA"
     gene            955..>1861
                     /gene="flhF"
                     /locus_tag="PLU43_08415"
     CDS             955..>1861
                     /gene="flhF"
                     /locus_tag="PLU43_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOO28475.1"
                     /translation="MIIKKFQGKTEEEAVLAAKKELGQNVVIMNVRKAKKKGFFSIFK
                     KPLVEVTAALEEEPEKAAPRPALTRSAITAKPEISSMAAIQEGSRSEKVLEEKLESLH
                     SLIEQQLVRENEEESENFKETEDKNVAFLKLIYNTLIDNEVAEKYANELIDEVEKIKK
                     PNVTIDFVLANIYQKMILKFGQPKVITPSRTNAKAVFFIGPTGVGKTTTIAKIASRLC
                     VNEKKKVALLTTDTYRIAAAEQLRTYANILDVPFRVIYSAEEITAAINDFKAYDFILI
                     DTAGHSHTNESQKNSMGQFIHCIDEA"
BASE COUNT          606 a          338 c          464 g          453 t
ORIGIN      
        1 gggcggcgac ttgcttgacc gcgtggtcat gatccgacga caggttgcat tggaactggg
       61 tacggtagtg ccgatcatcc gtttgagaga taacattcag ttaaatccga atcagtatat
      121 catcaagatc aagggaatac aggtcagtga gggagaaatc ctgtttgacc attatatggc
      181 aatgaatccg ggatttgtgg aagaagaaat cacgggtatt ccaacgtttg aaccgtcctt
      241 ccatctgccg gctatctgga ttacggagag ccagcgtgaa cgggcagaga gtatgggata
      301 tacagtggta gatccgcctt cgatcatagc aacgcatctg acagaggtca tcagacagca
      361 tatcagtgag ctgttgtcgc gtcaggatgt acagaatctt gtcaataata tcaaagagtc
      421 caatccttct cttgtagagg aactgacacc gaagctgctt ggacttggtg agatccagaa
      481 agtgctgcaa aacctgctgc gagagggtgt ttccatcaga gatcttctga cgatctttga
      541 gacacttgcc gatcatgcgg ctgtaacgag agacgccaat gtattgaccg agtatgtcag
      601 gcagagcctg aaacgtgcga tctcagcaaa atattttcct gcgaacgagt cgacaagtgt
      661 tgtgacactg gatccgaaga tcgaacagga aatcatgggc agtgtcaagc agacggagca
      721 gggagcttat ctgacattgg atccggagcg gacaaaaacg attatcaaag ctgtggaaaa
      781 ggaaatcaca aagcttgaaa atctgggtaa gaatccaatt ataattacat ctccgatcgt
      841 cagaatgtat tttaaaaaga tgacggaaga ctattttaaa gatttgattg tcgtatcata
      901 taatgacatc gacagtaatg ttgaattaca atctgtaggg atggtggcag cgtagtgatt
      961 attaagaaat ttcagggaaa aacagaagaa gaggctgttt tggcagcaaa aaaagaactt
     1021 ggccagaacg ttgtgatcat gaatgtcaga aaagcaaaaa aaaagggttt tttttctatt
     1081 tttaaaaaac cccttgtaga ggtgaccgca gctttggagg aagaaccgga aaaggcagct
     1141 ccaagaccgg ctctcacaag atctgctatt acggcaaagc cggaaataag cagcatggca
     1201 gctattcagg agggaagccg gagtgaaaag gtgctggagg aaaagctgga aagtcttcac
     1261 tcgctgatag aacagcagct tgtcagggaa aatgaagagg aatctgaaaa ttttaaagag
     1321 acagaagata aaaatgttgc ttttcttaaa ttgatctata atacactgat cgataatgag
     1381 gttgctgaaa aatacgcaaa cgaactgatc gatgaagtgg aaaaaatcaa aaagccaaat
     1441 gtgacgatcg attttgtgct tgcaaatatt tatcagaaaa tgattttaaa atttggacag
     1501 ccaaaagtta ttactccgtc cagaaccaat gcaaaagctg tgttttttat cggccctacg
     1561 ggagttggga agacgacgac gattgcaaag atcgcctcgc gcttatgtgt aaatgaaaaa
     1621 aagaaagtgg cgcttctgac aacggataca tatcgaattg ctgcagcaga gcagcttaga
     1681 acctatgcga atattctgga cgtgccgttt cgggttattt attccgcaga agaaatcacg
     1741 gcggcgatca atgattttaa agcgtatgac tttattctga tcgatactgc aggacattcc
     1801 catacaaatg aatcacagaa aaacagtatg ggacagttta tccactgtat tgatgaagcg
     1861 a
//
LOCUS       DAOSYE010000487         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52858_length_1858_cov_3.368275, whole genome shotgun sequence.
ACCESSION   DAOSYE010000487 DAOSYE010000000
VERSION     DAOSYE010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52858_length_1858_cov_3.368275"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLU43_08420"
     CDS             complement(<1..720)
                     /locus_tag="PLU43_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO28476.1"
                     /translation="MLNYKRYQKNPVIEYREREWPNKEITKAPVWCSVDLRDGNQALI
                     DPMIVDEKLEMFELLLKLGFKEIEIGFPAASQIEFDFLRQLVDRKMIPDDVWVQVLTQ
                     CREPLIDRTFESIQGIKNVIVHIYNSTSTLQRDVVFGMSKEEIIQIAVDGTRMVKERA
                     AKFPGNILLEYSPESFTGTEVEFALDICTAVQKEWGATKENPIILNLPATVELNTPNV
                     YADQIEWMNRHFTNRESIILSI"
     gene            1267..>1858
                     /locus_tag="PLU43_08425"
     CDS             1267..>1858
                     /locus_tag="PLU43_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOO28477.1"
                     /translation="MKFLTMIRIRTKLLIIVVFMVLGIVGVGLTGYFYNNKAAASLDE
                     VYAGNMVAIEVLGDLRTNSRANFANMLNLMVTEDADQKADVLANYEKRVAKIDTDLDS
                     FIDTGLDESENEQVAEMNTNLDAWDAVCAQIMSLVDEGNTADAIVLFKGSGEEVFETL
                     QTTIRNLETYQKEEGEKIYSESQANNKQGILLIIVIA"
BASE COUNT          546 a          400 c          363 g          549 t
ORIGIN      
        1 aatacttaaa ataatacttt ccctgtttgt aaagtgtctg ttcatccact cgatctgatc
       61 cgcataaaca ttcggcgtat tcagttccac tgttgccgga agattgagga tgatcggatt
      121 ttctttcgtc gcaccccatt ctttctgtac ggccgtacaa atatcaagag caaactcgac
      181 ttccgtacct gtaaagcttt ccggagaata ctccagaagg atatttcccg ggaatttggc
      241 agcacgctct ttgaccattc ttgttccgtc aaccgcgatc tggatgatct cctctttact
      301 catgccaaat accacgtccc gctgcagcgt agaggtggaa ttgtaaatat gcacaattac
      361 attttttatg ccctgaatag attcaaatgt acggtctatc aatggttccc ggcactgtgt
      421 cagcacctgt acccagacat catccggtat cattttccga tcaacaagct gacgcagaaa
      481 atcaaactct atctgtgaag ccgccggaaa tccaatttcg atctccttaa agccaagctt
      541 taaaagcagt tcaaacatct ccagcttttc atcaacgatc attgggtcga tcaacgcctg
      601 atttccgtct cttaaatcca cactgcacca gacaggtgcc tttgttattt ccttattcgg
      661 ccattctctt tcccggtatt cgattaccgg attcttctga tatcttttat aatttaacat
      721 ttaccgtttc ctccatattc tgattatgaa caattaaccg gcatattctc cgtgccagac
      781 agtctttacc tgaaaaacag ccggtccggg tcatacaggc cggaagatct ccccgctcga
      841 tctttccgtt tcgcctttta caggtttcgg agtcggtaaa ttacactata tttcacaata
      901 atcgacaatt tgtttctgac tattttttta gccccatttc tatcaacttc tttcattaga
      961 ttttcgatca gcctcctgcc ggatgatccg gtcaaaaggc taatggattc attatacatg
     1021 atagatttta aaaaatcaac aagcggcctg caaatttttt tgtaaaatca tggcgctaaa
     1081 accatgaaaa aagcaaaaat taactgacat ttcattgata atatgcgttt tatgtccaca
     1141 ttttacaaca aaaaacggag aaatctgaaa ttgactccca tattattaca tgctataata
     1201 atactagttt tcatatcgaa tcaatagttt ttatcaacaa cggaagaaaa taaacggagg
     1261 tgctgcatga aatttttaac gatgatcaga attagaacga aattattgat tattgtggtc
     1321 ttcatggttt tgggtattgt tggggtcggg ttaacaggtt atttctacaa taataaggca
     1381 gcggcttctt tggatgaagt ttatgccggc aatatggtcg caatcgaagt gcttggcgac
     1441 ctgcgtacaa attcaagggc aaattttgca aatatgctga atctcatggt tacggaagat
     1501 gcggatcaaa aggctgatgt actggcgaac tatgaaaaac gagtcgcaaa gatcgacacg
     1561 gatctggaca gctttatcga taccggcctt gatgaaagcg aaaatgaaca ggtggcggaa
     1621 atgaatacaa atctggatgc ctgggatgcg gtgtgcgcac agatcatgtc gcttgtcgat
     1681 gaaggaaata ccgcagatgc catcgtatta ttcaaaggca gcggtgaaga ggtatttgaa
     1741 acactgcaga caaccattcg taatttggaa acataccaaa aagaagaagg ggagaagatt
     1801 tatagcgaaa gccaggctaa taacaaacag gggatccttt taatcatcgt gattgcct
//
LOCUS       DAOSYE010000488         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52859_length_1858_cov_3.362174, whole genome shotgun sequence.
ACCESSION   DAOSYE010000488 DAOSYE010000000
VERSION     DAOSYE010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52859_length_1858_cov_3.362174"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLU43_08430"
     CDS             complement(<1..621)
                     /locus_tag="PLU43_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOO28478.1"
                     /translation="MTKIKLCGLSRDQDIFYANRLMPDYAGFVFCEKSSRSVTRRQAF
                     LLREKLHPSIKAVGVFVDETEETIRSLTVAGIIQLIQLHGGETEEEIRRLREILDPQV
                     KIIRAFRTDSAEDVVKAQKSAADLVLVDHGPGGTGQRFDWKLLAPLNRPFILAGGLHA
                     GNAAEAVRTLKPYAVDVSSGIETDGKKDFEKMEAFCRAVRTADMTGL"
     gene            complement(618..1409)
                     /gene="trpC"
                     /locus_tag="PLU43_08435"
     CDS             complement(618..1409)
                     /gene="trpC"
                     /locus_tag="PLU43_08435"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOO28479.1"
                     /translation="MKDILSTIAEYAQFRVAEAKKALPLKDVRRAALFEDCSTGFPFE
                     KALAGEEIGFICECKKASPSKGLIAKDFPYLAIAKEYEGAGADCISVLTEPKWFLGKN
                     EYLKEIAGQVRIPCLRKDFAVDEYMLYEAKLLGASAVLLICALLDMDRMREYIHICDE
                     LGLSALVEAHDETELEAAVAAGARIIGVNNRNLKDFTVDIHNSERLVRLAPKETLFVA
                     ESGIHTPKDIQILKESGVNAVLIGEALMRAKDKKEMLAYLKGKQV"
     gene            complement(1406..>1858)
                     /locus_tag="PLU43_08440"
     CDS             complement(1406..>1858)
                     /locus_tag="PLU43_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOO28480.1"
                     /translation="TYQLLGVYDEYLVEPLAKVLSSLGIKRGLVIYGQDKMDEISMSA
                     PTSVCEIRDGFYNRYTIKPEDFGFERCKKEELRGGTPEENARITRDILNGAKGPKRDA
                     VLLNAGAALYISEQAGSIREGIELAAELIDSGAAMKVLETFIRESNTL"
BASE COUNT          430 a          547 c          353 g          528 t
ORIGIN      
        1 aagccccgtc atatccgctg tgcggacagc tctgcagaag gcttccatct tttcaaaatc
       61 ctttttccca tccgtttcga tgccggagct gacatctacc gcatatggct taagagttcg
      121 gaccgcttcc gccgcattcc cggcatgcag tccgcccgca aggataaacg gtctgttaag
      181 cggtgcaagc agcttccagt caaaccgctg tcctgttccg ccgggtccgt gatcgaccag
      241 taccagatct gccgccgact tttgcgcttt tacaacatcc tccgcactgt ccgtcctgaa
      301 tgcccggatg atttttacct gcggatccag aatctccctc agcctgcgga tctcttcttc
      361 tgtttcccca ccatgcagct ggataagctg aataattcca gccacagtca gggagcggat
      421 cgtttcttct gtctcatcca caaaaacacc gaccgctttt atggaaggat gcagcttttc
      481 ccgcagtaaa aacgcctgcc tgcgggttac agatctgctg cttttttcac aaaacacgaa
      541 tcccgcgtaa tcaggcatta gccgattcgc ataaaaaata tcttggtctc tggaaagacc
      601 gcacaatttt attttcgtca tacttgtttt ccttttaagt atgcaagcat ctcttttttg
      661 tcttttgccc gcataagcgc ttctccgatc agcaccgcat ttacaccgct ttctttcaga
      721 atctgtatat cctttggtgt atggatcccg ctttccgcaa cgaacagcgt ctctttcggc
      781 gcaagcctga caagacgctc actgttatgg atatccacgg taaaatcctt caggtttcga
      841 ttattcacac caatgatcct tgcccctgcg gcaactgccg cttctaattc cgtctcatca
      901 tgcgcctcca caagtgctga taagccaagt tcatcacaaa tatggatgta ctctctcatc
      961 ctgtccatat ccagcagtgc acagatcaga aggactgcag atgcgccgag aagttttgct
     1021 tcataaagca tatattcatc cacggcaaaa tccttcctaa ggcatgggat ccgaacctgt
     1081 ccggcgatct ccttcaaata ttcatttttc cccaaaaacc acttcggctc cgtcaataca
     1141 gaaatgcagt ccgctccagc tccttcgtat tcctttgcga ttgccagata aggaaaatct
     1201 tttgcaatca gtccttttga cggcgatgcc tttttacatt cacagataaa accgatctct
     1261 tcaccggcaa gcgccttctc aaatggaaac cctgtgctgc agtcctcaaa aagagcagct
     1321 cttctgacat ccttaagcgg cagtgccttt ttggcctccg caacccgaaa ttgcgcatac
     1381 tcggcaattg tactcaaaat atctttcata aagtattgga ctccctgata aatgtttcca
     1441 gaaccttcat agccgcaccg ctgtctatca gttcggccgc cagctcgatc ccttccctga
     1501 tgctgcccgc ctgttccgaa atatacagtg cggcaccggc attcagtaaa acggcatctc
     1561 ttttaggtcc ctttgcgccg tttaaaatgt ctcttgtaat acgcgcattt tcctccggtg
     1621 ttccgccgcg cagttcttcc tttttacatc tttcaaatcc aaagtcttcc ggtttgatcg
     1681 tatagcgatt ataaaatccg tctctgatct cacagacact cgtcggtgca ctcatcgaga
     1741 tctcatccat cttatcctgc ccgtatatga caaggcctct ttttatacca agactgctta
     1801 aaacctttgc aagcggctct accaggtatt cgtcatatac gccaagcagc tggtaagt
//
LOCUS       DAOSYE010000489         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_52969_length_1856_cov_2.929484, whole genome shotgun sequence.
ACCESSION   DAOSYE010000489 DAOSYE010000000
VERSION     DAOSYE010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52969_length_1856_cov_2.929484"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="PLU43_08445"
     CDS             complement(<1..1842)
                     /locus_tag="PLU43_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOO28481.1"
                     /translation="MVIKLANGSFLRLMPVCGNIMRVTCSRETFFYDDSMLVEQEVFS
                     EKIPAVRIDELHTEKSKNIVSVQAGGLKMTIGLQDGALQAEDIKSKEVLLSCCRAEIS
                     KTMVMRYTTGGEKPVIERRKTVDGERNFIKNLKEVPDHEAYRAKLYFSFLEGEGIYGF
                     GQAEEGIYNYRKTHQYLYQHNMRIPMPFFYSDHGYGIFFDCCSLMTFQDDCDGSYVYM
                     DAVRQLDFYIIAGKSPDQVIGGLRFLTGKAAMLPKWAFGYIQSKEQYYTAKELADIVR
                     RYRKLAVPLDCVVQDWNTWEPGNWGEKLLDKTRYGDLKERIKEIRQMHAHAMISVWPN
                     MNTGGQNHKEFSDAGYLLHDDSTYDAFDEKARAMYWKQAKEELFNSGFEAWWCDSSEP
                     FSGPDWGGEVKREPWERFTLVGGEHKKYLPWESANIYALMHAKGIYENQRKETEEIRV
                     LNLTRSGYASGQKYAAVLWSGDTCASYETLKKQIIEGLNMGLSGYPYWTLDIGAFFTV
                     GDEWRNRGCGCKQDPTPKWFWKGMYNKGVKDKAYCELYVRWLQMGTFLPMFRSHGTDT
                     PREIWNFGKSGEPFYDAIEKSIRLRYRLMPYIYSMAGMVRHENEMIMR"
BASE COUNT          432 a          545 c          347 g          532 t
ORIGIN      
        1 ccgcatgatc atctcgttct catgcctgac catgcctgcc atcgaataaa tatacggcat
       61 cagacggtac cgcagccgta tggacttttc tatggcatcg taaaacggct ctccgctctt
      121 cccgaaattc cagatctccc gcggtgtatc cgtaccgtgc gaacggaaca tcggcaaaaa
      181 tgtccccatc tgcagccagc gtacatacag ttcacaatac gccttatcct ttaccccttt
      241 attatacatg cccttccaga accacttcgg cgtcggatcc tgtttgcatc cgcagcctct
      301 gttgcgccac tcatcaccta ccgtaaaaaa ggcaccgata tcaagcgtcc agtagggata
      361 tccggacaat cccatattca gtccttcgat gatctgtttt ttcaatgtct cataagaagc
      421 acaggtatct ccggaccaga gcactgcagc atatttctgt ccggatgcat atcctgagcg
      481 ggtcaggttc agaactctga tttcctctgt ttcctttctc tgattctcat atattccctt
      541 tgcatgcatg agcgcataga tgttcgctga ctcccacggc agatatttct tatgttcccc
      601 gcctacaagt gtaaaccgtt cccatggttc ccgttttact tctcctcccc agtccggtcc
      661 agaaaacggc tctgaggagt cacaccacca tgcttcgaat ccgctgttaa acagctcctc
      721 ttttgcctgt ttccagtaca tggccctggc tttttcgtca aacgcatcat atgtcgaatc
      781 atcatgcaaa agatatccgg catcagaaaa ttccttatga ttttgccccc ctgtattcat
      841 attcggccat accgatatca tggcatgtgc atgcatctgt ctgatctctt ttatccgttc
      901 ttttaaatcc ccgtagcggg tcttatcaag cagcttttct ccccagttcc ctggttccca
      961 cgtattccag tcctgtacga cacaatcgag cggcactgca agcttcctgt agcggcgcac
     1021 gatatccgca agctcctttg cggtataata ctgctctttt gactggatat acccaaacgc
     1081 ccatttcgga agcattgccg cctttcctgt cagaaagcgc agtccgccga tcacctgatc
     1141 agggcttttc ccggctatga tataaaaatc cagctgtctg accgcatcca tgtatacata
     1201 actgccgtca caatcatcct ggaacgtcat cagggaacag cagtcaaaga aaatgccgta
     1261 tccatgatca gaataaaaaa acggcatcgg gatccgcata ttatgctgat acagatattg
     1321 gtgtgttttg cggtaattat aaataccctc ttccgcctgc ccgaagccgt aaataccttc
     1381 tccttccaaa aaagaaaagt agagctttgc tctgtacgcc tcatggtcgg gcacttcctt
     1441 caggtttttt ataaaatttc tttccccgtc aacggtcttt cgtctttcga tcacgggttt
     1501 ttccccgccc gtcgtatacc gcatgaccat tgttttggaa atctccgcac ggcagcaaga
     1561 aaggagcacc tctttgcttt ttatatcttc tgcctgcagt gccccatcct gcaggcctat
     1621 cgtcattttc agaccgccgg cctgcacgct cacaatattt ttcgatttct cagtatgcag
     1681 ctcgtcgatc ctgaccgccg gtattttttc cgaaaacact tcctgttcga ccagcatgga
     1741 atcatcataa aaaaaagtct ccctgctgca ggtcactctc ataatattgc cgcataccgg
     1801 catcaaacgc agaaaactcc cgtttgcaag ttttattacc atttccctgt ccgtat
//
LOCUS       DAOSYE010000490         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_53286_length_1850_cov_3.395543, whole genome shotgun sequence.
ACCESSION   DAOSYE010000490 DAOSYE010000000
VERSION     DAOSYE010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53286_length_1850_cov_3.395543"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLU43_08450"
     CDS             <1..846
                     /locus_tag="PLU43_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28482.1"
                     /translation="YMVPGLILYTCFLIVPVIWSFYYTVFDGSPGIKWEFCGLDNYIK
                     LFSDKQFFQALIVNCKYIAIVMSGQMILGLALALMFHFWLQHCKSLVRTIVFFPVVLP
                     VVAVAQLFTKIYEVQPHYGLLNSVLANIGLEKYVQAWLGQESTALGSLSAMDIWTSIG
                     FYAIIFYGALLDVSQDVIEAARIDGAKAFQLLRFILLPLIRPIVVVCLIFSFTGTVKM
                     FDSALALTGGGPSGATKSLSLYMYNVAFTYSKVGYASIVAIVVFLICVIGSYFIKKLD
                     KDITA"
     gene            856..1707
                     /locus_tag="PLU43_08455"
     CDS             856..1707
                     /locus_tag="PLU43_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28483.1"
                     /translation="MMKKGLKIKKKIKRFFISLLIILVVFIDIYPLFWMITASLKEPS
                     EFVTKPAYALNSGFYFQNYIDAWTKGNMSLFFKNSLINTCVSILFITVITVTMSFALV
                     KMDWKWKKAMDQYVAFGIMVPFTTAMIPLFQIFSKMHLIDTRTCLILIYISTSISFSV
                     FLTSGYMRSLSDEILEAAVIDGCGIHGLLFRIVIPLIKNAIITNIVIQFFFKWNDLLA
                     AMTFVSSKKLKTVQTGLLYFSDEFGTKNWGAIFASVSICVIPMLIMYLFLNKRVIEGM
                     TAGATKG"
BASE COUNT          474 a          340 c          439 g          597 t
ORIGIN      
        1 tatatggttc ccggtctgat cttatatacc tgttttttga tcgtgccggt aatctggtca
       61 ttctactata cggtatttga cgggtctccc ggaatcaaat gggaattctg cggactggat
      121 aattatatca agctgttttc cgataaacag ttttttcagg ctttgatcgt gaactgcaaa
      181 tatatcgcaa tcgtcatgtc ggggcagatg attcttggac tggcattggc actgatgttt
      241 cacttttggc tgcagcactg taaatcgtta gtcagaacga tcgtgttttt tccggttgta
      301 ttgccggtcg ttgcggtcgc acagcttttt acgaaaattt atgaagtaca gccgcattat
      361 ggacttttaa acagtgtact ggccaacatt ggtctggaga aatatgtaca ggcatggctc
      421 gggcaggaga gcacggcgct tgggtcgtta agcgccatgg atatctggac gtcgatcggg
      481 ttttatgcca tcatttttta cggagcgctt ttggatgtgt cgcaggatgt catagaggcg
      541 gcgcgtatcg acggtgcaaa agccttccag cttcttcggt ttattctgct tccgttaatc
      601 aggccgatcg tagttgtctg cctgatattc agctttacgg gaacggtaaa aatgttcgat
      661 tcggcgctgg cgctgaccgg cggtggtcca agcggtgcta caaagtcgct ttccctgtat
      721 atgtataacg tcgcatttac gtatagcaaa gtaggctatg caagtattgt tgcgatcgtt
      781 gtatttttga tctgtgtaat aggctcttat ttcattaaaa aactggataa ggatattacc
      841 gcatagggag ggaaaatgat gaaaaaaggt ttaaaaataa agaaaaaaat caaacggttt
      901 tttatttcac tgcttattat tttagttgtt tttattgata tttatccgtt gttctggatg
      961 atcaccgcat ccttaaagga gccttcggaa tttgttacaa agccggcata cgcacttaac
     1021 agcggttttt attttcaaaa ttatattgat gcatggacga aaggaaatat gtcgctgttt
     1081 tttaagaaca gtctgatcaa tacgtgcgtg tcgattttgt ttattacggt cattacggtc
     1141 accatgtcgt ttgcacttgt gaaaatggac tggaaatgga aaaaggcaat ggaccagtat
     1201 gttgcgtttg gtattatggt tccgtttacg acggcaatga ttccgctgtt tcagatcttc
     1261 agtaaaatgc atctgatcga tacaaggacc tgcctgatac tgatttatat ttccacgtcc
     1321 atttcgtttt cggtctttct gacaagcgga tatatgcggt cattgtcgga tgagatatta
     1381 gaggcggctg tcattgacgg atgcggaatc catggtctgc tgtttcgcat cgtgattccg
     1441 cttataaaaa atgcaattat aaccaacatt gtaattcagt tcttctttaa gtggaatgac
     1501 cttttagcgg ccatgacgtt tgtcagcagc aagaagctga aaacagtgca gaccggactg
     1561 ctttattttt cggatgaatt cggaacaaaa aactggggtg ctattttcgc atcggtatcg
     1621 atctgtgtca ttccgatgct gatcatgtat ttgtttttaa ataaacgtgt cattgaaggc
     1681 atgactgccg gggcaacaaa ggggtgagaa ctatgaaaga gttaaattat gaacagatca
     1741 tcagaaacta tattcatcag aacattcata cggtcttaaa ggagccttat tcttttatcc
     1801 gttatccgtt cattgatccg ggctccgtat ataacggaaa cgtgtgggac
//
LOCUS       DAOSYE010000491         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_53292_length_1850_cov_3.096379, whole genome shotgun sequence.
ACCESSION   DAOSYE010000491 DAOSYE010000000
VERSION     DAOSYE010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53292_length_1850_cov_3.096379"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLU43_08460"
     CDS             complement(<1..264)
                     /locus_tag="PLU43_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28484.1"
                     /translation="MIDRKQILSFSHYKKGKAYTGSQKGMRYRIQKKESKGEEQFCVD
                     VWPEPFCFEKTDQKQITEAVFSFSEEGYEQVLKYLNDRYEERTW"
     gene            complement(294..809)
                     /locus_tag="PLU43_08465"
     CDS             complement(294..809)
                     /locus_tag="PLU43_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO28485.1"
                     /translation="MKCEVIFDSKTGNTRALAKEFYKCLACGEKEIQELCEETDPEQG
                     EVYFVGFWTDRGTCSMEVIDFLSKLQGKYVALFGTCGMGNNPEYYRDIENRVKVWLPE
                     DNQYLGCFICQGKMPIQVRKRYETLLEEGNQREMAERMLRNFDEVMLHPDSDDFKKAA
                     EFVEKVFKKIR"
     gene            complement(1450..1850)
                     /locus_tag="PLU43_08470"
                     /pseudo
     CDS             complement(1450..1850)
                     /locus_tag="PLU43_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
BASE COUNT          452 a          502 c          319 g          577 t
ORIGIN      
        1 ccatgttctt tcttcatacc ggtcgttcaa atatttcaaa acctgctcat agccttcttc
       61 cgaaaaagaa aatactgctt ccgtaatttg cttttgatct gttttttcaa aacagaacgg
      121 ctccggccat acatccacgc aaaactgctc ttcacccttg ctctcttttt tctgaatacg
      181 atagcgcatt cctttctgac ttcctgtata tgcttttccc tttttataat gactgaatga
      241 taagatctgc tttctgtcta tcatctgttc ccccttcaga ccacaaaata tttctaccta
      301 atttttttaa atactttttc aacaaattcc gctgcctttt taaagtcatc gctatccgga
      361 tgcagcatga cttcgtcaaa attacgaagc atccgctctg ccatctcacg ctgatttcct
      421 tcttccagca gcgtttcata tctcttccgc acctggatcg gcatcttgcc ctgacagata
      481 aaacagccca gatactgatt atcctcaggc agccatactt tcaccctgtt ttctatatca
      541 cggtaatact ccgggttgtt tcccattccg catgtaccaa aaagcgccac atattttccc
      601 tgtagttttg aaagaaaatc aatgacttcc atactgcagg tacccctgtc agtccagaac
      661 ccgacaaaat aaacttctcc ctgttccgga tccgtttcct cacacagctc ctgaatttct
      721 ttttccccgc atgcaaggca cttataaaat tccttcgcaa gagctctcgt atttcctgtt
      781 ttactgtcaa atattacttc gcacttcata ggcgccacct catttaaatc gaattgatgc
      841 tgtctacata gtaagacaaa agcggctgga attcaaatga agttttcata atatttataa
      901 atattatata aaaagaaagc aatatatgat aaataatcgc ttttatccac acatattgct
      961 ttcttgtcta tttatatgat ccattcacgc ttcttatttc ttcagaaaat ccgggatctt
     1021 gatcgacttc acctcgacct tgctctcaag cggtgcctga ggtctctgaa taccggacat
     1081 ggaagacatc ggtctctgtg tcggtgccga aggtgtcggc aaaggctgtc cctgcgtcgg
     1141 tgtcggcatt ggtgtatgta ccggcatctg ctgcatcggc cgctgctgca tattctgcat
     1201 attctgatat ccattattca tcggctgctg tccatacata tcctgacgcg gaatattacc
     1261 ctgcatcggc atgtttccct gcggcatctg attcatctga ggattcatcc cattattcat
     1321 attcatattg ttgccgttca taccgccctg catcggagct cccatacctg cattcggaga
     1381 tacgatcggt cttctctgat atgcggagga ctgtacataa cgtcccatac cctgcggctg
     1441 gctgttctgt gccgaattct cgctcaatcc tgtcgctatg acggttaccg tacatgtatc
     1501 cggctctttg tcatcacaca tcgcacccat gatcagattg acatcatcac ctgtcaggtc
     1561 acgcacaaac gataccgcat catctgcgtc aagcagtgta atttcaccgg aaatattgac
     1621 gatcacatgg gaagcaccct gaatcgtcgt ctcaagaagc ggactgttaa cagcctgctt
     1681 tacagcttca atcgctttat catcaccctt tgccgcgccg attccaatat gggcaattcc
     1741 cttattttcc ataacagtac ggacatcggc aaaatcaaga ttaatcaggg ccggtacgtt
     1801 gatcagatcc gtaattccgc ggaccgcctg ctgtaatact tcgtctgcct
//
LOCUS       DAOSYE010000492         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_53636_length_1843_cov_2.608501, whole genome shotgun sequence.
ACCESSION   DAOSYE010000492 DAOSYE010000000
VERSION     DAOSYE010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53636_length_1843_cov_2.608501"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1843
                     /locus_tag="PLU43_08475"
     CDS             <1..>1843
                     /locus_tag="PLU43_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO28486.1"
                     /translation="VFYISVGILFFSITTFIEANRTGRFISCSVVMSLVLNFLFLPML
                     FIYYGNTLCCIPVFFMIGLLYTILLLEGKSALIMTGTQALFYFLLLIFGTKIPYDSSA
                     AEKYGLLEYADVFVAIVFAGICGGLAVRYHLILYQKEQEKSEQYHMEAMDAYIAKDIF
                     LINMSHEIRTPMNAIVGTVDLLLDQDINERVRDSVYNILNSCNALLSITNELMDLSKS
                     ESREVAVYVARYDIGELLMEIINMISVRLMYSEVELFVEIDEKIPRYLYGDSSRLRQV
                     FINILNNAVKYTKRGKIMLRVGYKKLTVNRISLFAEVEDTGAGIKAENIPKLFKVYQR
                     VEEEDSEKRNVEGTGLGLSICREILDKMDGEIYVRSEYHVGSTFYFNIPQQVDAGELL
                     ADVESAELLHVLVFEKDTDARENIGKILDRLNIRADYPGNRKEFETCMMDHDYTHLFV
                     SYDKYIENVRFLDRNLMREKLVIISEINQMLSVNKFGCILMRPVHVMNVAAVFRNENN
                     SFVREIIKKGGFICPAATILVVDDNLTNLNVAGGLLKKYEANVITALSGRECLNILEN
                     QDVDMIFLDYMMPEMNGIDTLDNIRRMSRPRMKTLPVIALTANVVNGA"
BASE COUNT          527 a          292 c          474 g          550 t
ORIGIN      
        1 cgtattttat atttcggtag ggattctgtt tttttcgatt acaacattta tagaagcgaa
       61 tagaacgggc agatttattt cctgttcagt tgtcatgtct cttgtactca attttctgtt
      121 tcttcctatg ctgtttattt attatgggaa cacgctctgt tgtattcctg ttttttttat
      181 gatcgggctg ctgtatacga tactgcttct tgaaggaaag tctgcgctta ttatgaccgg
      241 aactcaggca cttttttatt ttttgctgct gatatttggg acgaaaatac cgtatgattc
      301 gtctgcagcc gaaaaatatg gtctgctgga atatgcggat gtgtttgttg caattgtttt
      361 tgcagggatc tgcggaggac ttgcggtcag ataccatctg attctgtatc agaaagagca
      421 ggaaaaatcg gaacagtatc atatggaagc aatggacgcc tatattgcga aggatatttt
      481 tttgatcaat atgtcccatg agatcagaac gccgatgaat gcaattgtcg gaacagttga
      541 tcttttgctg gaccaggata tcaatgaacg ggtcagggac agcgtctata atatattgaa
      601 ttcctgcaat gccctgcttt ccataacaaa tgagctgatg gatctgtcta aatcagaaag
      661 ccgtgaagtt gctgtttatg tagcaagata tgatattggc gagctgctga tggagattat
      721 caatatgata tccgtcagac tgatgtacag tgaggtggag ctgtttgttg aaatcgacga
      781 aaagattccg agatatcttt atggcgacag ttcaaggctt aggcaggtgt ttatcaatat
      841 tctgaataat gcggtcaagt atacgaagag ggggaaaatc atgcttcgtg tcggctataa
      901 aaagctgact gtgaatagga tcagtttgtt tgcggaagta gaagataccg gagcgggtat
      961 caaggcagaa aacattccaa agctttttaa agtataccag cgtgtggaag aagaggacag
     1021 cgagaaaaga aatgtggaag ggaccggact tggtctgtcg atctgcaggg aaattcttga
     1081 caaaatggat ggagaaatct atgtgcgcag tgaatatcat gtaggcagta cattttattt
     1141 caatattccg cagcaggtcg atgccgggga actgctggct gacgtagaga gtgcggaatt
     1201 gctgcatgtt ctggtatttg aaaaagatac tgatgcgcgt gagaacatag gaaaaatact
     1261 ggatcgctta aacatcaggg cagattatcc gggaaacagg aaggaatttg agacctgcat
     1321 gatggaccat gactatacac atctgtttgt atcatatgat aagtatatag agaatgttcg
     1381 ttttctggat cgaaatctta tgcgtgaaaa acttgtgatt atttctgaga tcaatcagat
     1441 gctgtctgtc aataaatttg gctgtatcct gatgcggccg gttcatgtga tgaatgtggc
     1501 ggctgttttc agaaacgaaa ataacagttt tgtaagggaa attattaaaa agggaggttt
     1561 tatctgtcct gcggcgacaa ttcttgtagt ggatgataat ctgacgaatc tgaatgtggc
     1621 aggcggactg ctgaaaaaat atgaggcaaa tgttattacg gccttaagcg gcagagaatg
     1681 tttgaatata ttggaaaatc aggatgtgga tatgatattt cttgactata tgatgccgga
     1741 gatgaacggg atcgatacgc tggataatat cagacggatg agccgtccgc gtatgaagac
     1801 gctgccggtg atcgccctga cggcaaatgt agtaaacggt gcc
//
LOCUS       DAOSYE010000493         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54157_length_1832_cov_4.292628, whole genome shotgun sequence.
ACCESSION   DAOSYE010000493 DAOSYE010000000
VERSION     DAOSYE010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54157_length_1832_cov_4.292628"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..511)
                     /locus_tag="PLU43_08480"
     CDS             complement(74..511)
                     /locus_tag="PLU43_08480"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOO28487.1"
                     /translation="MKKLKDAARRIKRDIWNAKWALLALICYLILMNVLFGTGCSIKA
                     ITGVPCPGCGMTRAFFYLLSGKFALSFQMHPLLLLWLLYGLYLAVMRYWYGKRPKGML
                     PILFVLCALMFAVYVYRMAVWFPNQDPMVYGRPHGVLYKLIEK"
     gene            611..1498
                     /locus_tag="PLU43_08485"
     CDS             611..1498
                     /locus_tag="PLU43_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOO28488.1"
                     /translation="MLNGWLIVNRFLNTAKFREIYVWLAESAKKQQIRLTFMTNADFL
                     VRADTGELITKAFSEQQPDFVLFWDKDIRLARALEKKGLRLFNRADAIEACDDKSLCA
                     VKLAGIVKMPRTYVVPMTYSNIGYTDFAFLTDIEKELSYPYIIKECFGSFGMQVYLAH
                     TRTEAAELLSKNNKGTPYIFQEYISTSTGRDVRLHVVGNKVVTAMLRYNENDFRANIT
                     NGGSMKPYRPTSEQIRLSLQVCSELQLDFAGVDILFGPDEEPLLCEVNSNAHFKNIYD
                     CTGINIADAIMKHIRTVCQ"
     gene            1549..>1832
                     /locus_tag="PLU43_08490"
     CDS             1549..>1832
                     /locus_tag="PLU43_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28489.1"
                     /translation="MYIKKGYLLYEPKDIKRNAGFIELFLAEARQKNITLSLLYANDQ
                     RLLRAKKTDEHSLADALGNPDFIISRSRTPSLSKMLEQSGIRVYNNADIC"
BASE COUNT          541 a          447 c          371 g          473 t
ORIGIN      
        1 ttcattaatg tgatacttct atcctatcat attttgataa tttttcaaaa catttccgca
       61 aattcccttg tgattatttt tcgataagct tgtataatac gccgtgcggc cttccataaa
      121 ccattggatc ctggtttgga aaccagacag ccatacgata aacataaact gcaaacatca
      181 gggcgcacag cacaaaaagg atcggaagca ttccttttgg tctctttcca taccagtagc
      241 gcatgactgc aagatacaat ccgtagagca gccacaaaag cagcagcgga tgcatctgaa
      301 aagacagcgc aaacttcccg gaaagcaaat aaaaaaacgc tcttgtcatc ccgcagcccg
      361 gacagggaac cccggttatc gccttgatcg aacagcccgt accaaacaga acattcataa
      421 ggatcaaata gcatattaga gccaggagcg cccattttgc attccaaata tctcttttta
      481 tacgccttgc ggcatccttc aattttttca tttctttgtc ccgccatact ctttttcaca
      541 ttgcaagaac cgctttggta ttgtagaata gggatgatcc ttatacgcga aagaaaagag
      601 gccctgcttt atgctcaacg gatggttaat cgtaaaccga tttttaaata cggcaaaatt
      661 ccgggaaatc tatgtctggc tggcggaatc agcaaaaaaa cagcagatcc ggctgacttt
      721 catgacaaat gccgattttt tagtccgggc agataccggc gaacttatca caaaggcttt
      781 ttcggaacaa cagccggatt ttgtcctgtt ctgggacaaa gatatccggc ttgcccgtgc
      841 ccttgaaaag aaaggactca ggctctttaa ccgtgcggat gccatcgaag cctgcgacga
      901 taaatcactg tgtgccgtca agcttgcagg tatcgtaaaa atgccccgca cttatgtcgt
      961 tccaatgact tattccaata tcggctacac agactttgcc tttctcacgg atatcgaaaa
     1021 agaactttcc tatccttata tcatcaaaga atgcttcggc tccttcggta tgcaggtcta
     1081 tctcgcccac acacgcaccg aagcggcaga gctgctgtcc aaaaacaata agggaactcc
     1141 atacatcttt caggaatata tcagtacaag caccgggcgg gacgtccgtc ttcatgtcgt
     1201 cggaaataaa gtcgtgaccg ccatgctaag atataatgaa aacgattttc gcgcaaatat
     1261 tacaaacggc ggttccatga aaccctatcg gccgacaagt gaacagatca ggctttccct
     1321 gcaggtctgc agcgaattac agctggattt tgccggtgtc gatattctct ttggcccgga
     1381 tgaagaacct cttttatgtg aagtcaactc caatgcacat tttaaaaata tttacgactg
     1441 taccggcatc aatattgctg atgcaattat gaaacatatc cgcacagtct gtcaatagtt
     1501 accggccatt cggatatatg attccaaaca ataaacggag agttgtttat gtatataaaa
     1561 aaaggatatc tgctctatga gccaaaagat atcaaaagaa atgccggctt tatcgaactg
     1621 tttcttgcgg aagcccggca gaaaaatatc acgctgtcac tgctgtatgc aaacgatcag
     1681 cgcctgctgc gggcaaaaaa aacggatgag cactcactcg cagacgccct tggcaatccg
     1741 gatttcataa tcagccgttc ccgtactcct tcgctttcca aaatgctgga gcaaagcggt
     1801 atccgtgttt acaacaatgc ggacatatgc ag
//
LOCUS       DAOSYE010000494         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54253_length_1830_cov_3.436056, whole genome shotgun sequence.
ACCESSION   DAOSYE010000494 DAOSYE010000000
VERSION     DAOSYE010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54253_length_1830_cov_3.436056"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PLU43_08495"
     CDS             complement(<1..1270)
                     /locus_tag="PLU43_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOO28490.1"
                     /translation="MSSEVFSEKAKHKRKSMLFRVLFSRTMVMFILLLVQILFLTAVF
                     WELGSYSSYILIACNIIGALLVIFLISSNGRAEFEFIWVVLICVLPVFGVLLYLFVLL
                     NPGGMGLKKLLDRRIQETKEYLITEKQVQKKLSKSKKIISNIAWYLQYTGGYPTYQNT
                     AVKYYPLGEDNYRDLLLELKKAEKFIFIEYFIIDEGIVWNSILEILKEKVSAGVEVRV
                     MYDGTCTVALLPWSYPKTLRSYGIKAKVFAPIRPMLSTHQNNRDHRKILVIDGKIGFT
                     GGVNLADEYMNFKELYGHWKDTAIRLTGDAVKTLTLLFLQTWNMTEKGEEDYAAYLMQ
                     PGASFCDTGKKQKYFVIPYGDDPTNHEDIAEDVYRYLVNEAKRYVHIMSPYFIVDSEM
                     LSAITFAAKRGVEVRLLLPHIPDKRVPFMMA"
     gene            complement(1267..>1830)
                     /locus_tag="PLU43_08500"
     CDS             complement(1267..>1830)
                     /locus_tag="PLU43_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28491.1"
                     /translation="EFGIIKTVMSMMQFPFRFLTIATGTLSFCCGLAIIKSDFLKRYK
                     KVLFAMLLVLSLIGGYEILDEYIRQDVYITNISGGFSEAELPEYWPENTDSEIFSDTS
                     PWKNNVYLFDYKKEGTTITFSYHTDADDAFVSLPLLCYPGYYAVLDDGMQLRVDKGEK
                     NRVCVYLPKNTETGTVTLKYDFWRLFK"
BASE COUNT          540 a          471 c          306 g          513 t
ORIGIN      
        1 ttgccatcat aaacggaact ctcttatccg gaatatgcgg cagcagaagc ctgacctcca
       61 ctcctctttt tgccgcaaac gtaatcgcgg aaagcatttc actgtccact ataaaataag
      121 ggctcataat atgaacatat cttttcgcct cattgaccag ataccgatac acatcttccg
      181 caatatcttc atggttcgtc gggtcatccc cataaggaat cacaaaatac ttttgtttct
      241 tccccgtatc acaaaaagaa gcgccgggct gcatcaggta tgccgcataa tcctcttctc
      301 ccttttccgt catgttccac gtctgtaaaa acaacagtgt cagtgttttt acggcatctc
      361 ctgtcagcct gattgccgtg tctttccagt gtccgtaaag ttctttaaaa ttcatatatt
      421 catcagccag attgacgccg ccggtaaatc caatcttccc gtcgatcaca agaatctttc
      481 tgtggtccct gttattctga tgtgtggaaa gcatcggtct gatcggtgca aataccttgg
      541 ccttgatgcc ataagagcgc agcgttttcg gatagctcca cggaagcagc gccacagtac
      601 acgtaccgtc atacatcaca cgcacttcaa cacctgcaga taccttttcc ttcaaaattt
      661 ccagaatgga attccagaca atgccttcat caataataaa gtattcaata aaaataaatt
      721 tctccgcttt ttttaattcc aaaagcagat ccctgtaatt gtcctccccg agcggatagt
      781 atttaacagc agtgttctga taagtcggat aaccgcccgt atactgcaga taccacgcaa
      841 tattggagat tatcttctta ctcttcgaca gctttttctg tacctgcttt tccgtgatca
      901 aatattcttt cgtctcctgg atgcgccggt caagcaattt tttcagaccc ataccgcctg
      961 gattcagcag cacgaacaaa tacagcagta caccgaaaac cggaagcaca caaatcaaaa
     1021 cgacccatat aaattcaaat tccgcacgtc cattgctgct gatcaaaaaa ataacaagca
     1081 gcgcgccgat aatattacat gcgatcagaa tatagctgct atagctgccc aactcccaaa
     1141 aaaccgccgt caaaaataag atctgaacca aaagcaaaat aaacatcacc attgtccggc
     1201 tgaataaaac cctgaacaac attgattttc ttttatgttt tgctttttcg gaaaaaactt
     1261 cactgctcac ttaaaaagcc tccagaaatc atattttaag gtaacggtcc ctgtctcagt
     1321 attcttcggc agataaacgc acaccctgtt cttttctcct ttgtcgaccc taagctgcat
     1381 cccgtcatca agaacggcat aatagcctgg gtaacataaa agcggcagtg atacgaacgc
     1441 atcatcggca tcagtatgat atgagaaagt gatcgttgtc ccttcttttt tataatcaaa
     1501 cagatacaca ttatttttcc atggactcgt atctgaaaat atctcactgt ccgtattctc
     1561 cggccagtac tccggaagct ccgcctcgga aaatccgccg cttatatttg taatatagac
     1621 atcctgcctg atatattcat ccagaatttc atagcctcca atcagactta gaacaagcag
     1681 catggcaaac aatacttttt tatacctttt caggaaatcc gacttgataa ttgcaagtcc
     1741 gcagcaaaaa gagagtgttc ctgttgcaat cgtcaaaaat cggaacggaa actgcatcat
     1801 actcattaca gttttgataa tgccaaattc
//
LOCUS       DAOSYE010000495         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54262_length_1830_cov_2.805634, whole genome shotgun sequence.
ACCESSION   DAOSYE010000495 DAOSYE010000000
VERSION     DAOSYE010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54262_length_1830_cov_2.805634"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLU43_08505"
     CDS             <1..735
                     /locus_tag="PLU43_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28492.1"
                     /translation="STSYVNISDVSNKRLFILGTDNFGRDVLTELISAIKTSLKIGFI
                     AGLIATLIGLSFGLLAGYIGGFIDDLIMFVTNLFTVIPSFILLILISYSVGKNARGVM
                     MVAVIIGITAWPWTTRSVRSQVISLRNRDHVNLSKLSGHSLFRIILTDILPYIASYVV
                     MALILQVSSGILAEAQLSMIGLGPATTTVSTLGLMMNWAMSYSAHLNGDWWAYFPVIL
                     SIALISFSLNLMNTGLDQVFNPQLRS"
     gene            749..1756
                     /locus_tag="PLU43_08510"
     CDS             749..1756
                     /locus_tag="PLU43_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28493.1"
                     /translation="MGKIMLEVEHLKTKYITRFGEDIYAVDGVSLQIEEGKSLGIAGE
                     SGCGKSTLALSLMGYYFAPLHYQSGTIKVDGVNISGMKADDIRRKILGNEIAYIPQAA
                     MNALNPTQKIMNFIEDVIHAHEPKASKKDIYDLAKERFQILGLPENVLQKHAVELSGG
                     MKQRTVIAISTILSPKVLIADEPSSALDVTSQKMVIKMLRDLMDKGIIKSMIFITHEL
                     PLLYNVTDDIMVMYAGQIVERATAKEMVFDPLHPYSKGLMGSIIVPEEGTRDMKLTAI
                     PGTPPNLKNPPKGCRFADRCRYAKPECMLHGIAESHLDNGRMFRCYMTEKELREVYDS
                     E"
BASE COUNT          499 a          357 c          477 g          497 t
ORIGIN      
        1 tctacctctt atgtaaatat cagcgatgtg agcaataaaa ggctgtttat tcttggaacc
       61 gataatttcg gacgcgacgt tctgacagaa ctgatttctg caatcaaaac atcgcttaag
      121 atcgggttta ttgcaggact gatcgcaacg ctgatcggct tgtccttcgg gctgcttgcc
      181 ggatatatag gcggatttat tgacgatctg atcatgttcg ttacaaatct gtttacggtc
      241 atcccgagtt ttattttgtt gatactgatt tcatacagtg tcggcaaaaa tgcgagaggc
      301 gttatgatgg ttgcagttat tatcggcatt acggcttggc cgtggacgac gagatccgtg
      361 cggtcacagg tcatttccct tcggaacagg gatcatgtaa atctgtcgaa gctgtccggc
      421 cacagtttgt ttcggatcat tctgacggat atcctgccgt atatcgcttc ctatgttgtt
      481 atggcactga tcctgcaggt ttcttccgga attctggccg aagcacagct ttccatgata
      541 ggacttggcc cggccactac gactgtatcc acgcttggtc ttatgatgaa ctgggcgatg
      601 tcatacagcg cgcatctgaa tggggactgg tgggcatatt ttcctgttat tctttccata
      661 gcgttgattt cattttcact gaatctgatg aatacaggtc tggatcaggt atttaatccg
      721 cagctgagat cgtaaaggag agaaaagtat gggaaaaata atgctggaag tggaacattt
      781 aaaaacaaaa tatattacaa gattcggaga ggacatctat gctgtggacg gggtgtccct
      841 tcagatagaa gaaggaaaat ctcttggcat tgcaggggaa tccggatgtg gaaagtccac
      901 actggcctta agcctgatgg gatattattt tgcgccgctt cattaccaga gcggaaccat
      961 caaggtggac ggggtcaata tttccggaat gaaggcagat gatatccgca gaaaaattct
     1021 tggaaatgaa attgcctata ttccgcaggc ggcgatgaat gcgctgaatc cgacacagaa
     1081 gatcatgaat tttattgagg atgttattca tgcgcatgaa ccgaaggctt ccaaaaagga
     1141 tatttatgac cttgcaaagg aacggttcca gatactcggg ctgcctgaaa atgtattgca
     1201 gaagcacgca gtggagcttt ccggaggtat gaaacagaga acggtcattg caatatccac
     1261 gatcctgtcg ccgaaagtgc tgattgcaga tgaaccatct tccgcacttg acgtaacttc
     1321 gcagaaaatg gtcattaaaa tgctgcggga tctgatggat aaagggatca tcaaatccat
     1381 gatctttatt acgcatgaac ttccattgct ttataatgtg accgatgata ttatggtcat
     1441 gtatgctggg cagatcgttg aaagggcaac tgcaaaggaa atggtgtttg atcctcttca
     1501 tccgtattcc aagggcctga tgggttccat tatcgtaccg gaggaaggta cgagggacat
     1561 gaagctgact gcaattccgg gtactccgcc gaatctgaaa aatccgccga aaggatgcag
     1621 gtttgcggat cgctgcaggt atgcgaagcc ggaatgtatg cttcatggaa tcgcggaaag
     1681 tcatttggat aacggccgga tgtttcgctg ttatatgact gaaaaagaat taagggaggt
     1741 gtatgacagt gaatgaaaag gaaaaaaagt ctgaacaggc gctgtgcctg agcggaaagg
     1801 gagttacaaa ggtctttggt ttcggaaacg
//
LOCUS       DAOSYE010000496         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54359_length_1828_cov_2.623801, whole genome shotgun sequence.
ACCESSION   DAOSYE010000496 DAOSYE010000000
VERSION     DAOSYE010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54359_length_1828_cov_2.623801"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PLU43_08515"
     CDS             <1..1706
                     /locus_tag="PLU43_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO28494.1"
                     /translation="LFLFLLATGQDTYYKGIISELLILTAVFMNIRLGNRGYHLSLVL
                     LVFSSVTSLFPILLHGKMSSISGVIVGLGTIVVCSLIHSYQKLIKQKMNGEREKNEEL
                     SALYEEITATEEELRQQNEMLQKYNQVITENEEKLNRLAYYDKLTGLPNRNGFLEKLR
                     TVVYEAEKTGMEFAVIFIDLDNFKLINDIRGRDVGDFLLKEEAQRLKSFCRNEDYIGR
                     LDGDEFGLILDGNFTSEQITEEIKNLEALIRKSLRVSEMEISTSASFGFSLYPKDGTN
                     VSELMRCSDMAMYYAKKVGKSGIQNFSSSMEKEILDKQKMESQLAFALEKEEMYLVFQ
                     PQFELDGKRLCGFEALVRWKSSVLGNVGPGDFIPVAEKNGFIIVLGEWIIRKACLFAK
                     MIKEQYQSPVMLAINISPVQIMDSGFLNKVYKIFEETGADPGLIEFEITESAFIASMD
                     IAVKKLQEIKEFGVKIALDDFGYGYSSLNYLQMLPIDILKIDKIFCDAIMREPGDLHV
                     AGPIIDLMHQMNLKVIAEGVEEQQQLLYLENRKCDIIQGYVWGKPLPEAEVRKLLLEG
                     I"
BASE COUNT          554 a          285 c          460 g          529 t
ORIGIN      
        1 tgctgtttct ttttttgctg gcaacaggac aggatactta ttataaggga atcatttccg
       61 aattattgat attaacagcc gtatttatga atattcgtct tggaaacagg ggatatcatc
      121 tttctctggt tctgcttgtt ttttcatccg taacttcctt gtttcccata ttgctgcatg
      181 gaaagatgag cagtatttca ggtgtgatcg tcggcctggg aacgatcgtg gtctgcagtc
      241 tgattcattc ttatcagaaa ctgatcaaac agaaaatgaa tggggaacgt gagaaaaacg
      301 aagagctttc cgctctgtat gaagagatca ctgctacaga ggaagaattg cggcagcaga
      361 acgaaatgct tcaaaaatat aatcaggtga ttacggaaaa tgaggaaaaa ttaaaccgcc
      421 ttgcgtatta tgataaactt accggtctgc cgaaccgtaa cgggtttttg gaaaaactgc
      481 ggacagttgt ctatgaagcg gaaaagacgg gaatggaatt tgcagttatt tttatcgatc
      541 tggataattt taaattgatc aacgatatca gagggcgtga tgttggtgat tttttattga
      601 aggaagaggc gcaaagactg aaatctttct gccgtaatga agattatatc ggcagactgg
      661 acggagacga gtttggtctg attttggacg gaaattttac aagtgaacag attacggaag
      721 aaatcaaaaa tctggaagct ttaatcagga aaagtctgcg ggtttctgaa atggagatca
      781 gtacaagcgc aagctttggt ttttctcttt atccaaagga cggaacgaac gtttccgaac
      841 tgatgagatg ttccgatatg gcaatgtatt atgcgaaaaa agttggtaaa agcggtatcc
      901 agaatttcag cagttctatg gaaaaagaaa tattggacaa acagaaaatg gaaagtcagt
      961 tggcatttgc attggaaaaa gaagaaatgt atcttgtttt tcagccgcag tttgaactgg
     1021 atggaaaaag actgtgcggg tttgaggctc tggtcagatg gaaatcatct gttttaggga
     1081 atgttggtcc aggtgatttt attccggttg cggagaaaaa cgggtttatt attgttcttg
     1141 gagaatggat tatcagaaaa gcatgtcttt ttgcaaaaat gatcaaagag caataccaat
     1201 cccccgtgat gcttgcgatc aatatatctc cggttcagat tatggattcc gggtttttga
     1261 ataaggtcta taagatcttt gaagagacag gggcagaccc tggtctgata gaattcgaga
     1321 tcacagagtc ggcatttatt gcgtcaatgg atattgcagt taagaagctg caggaaataa
     1381 aggaattcgg agttaaaatt gcgcttgatg attttggtta cgggtattct tccctgaact
     1441 atctccaaat gctgccgatc gatattttaa agatcgataa gatcttttgt gatgcgatca
     1501 tgagagagcc tggggatctg cacgttgcgg gtccgatcat cgacctgatg catcagatga
     1561 atctgaaggt gattgcagaa ggggttgaag agcagcagca gcttttatat ttggaaaatc
     1621 gaaaatgcga tattatacaa gggtatgtat gggggaagcc gcttccggag gccgaagtta
     1681 gaaagctgct gcttgaaggc atataatttt tgaatttaat aaaatacata gttattaagc
     1741 agagtcacgg acagaaatgt caggcggctc tgctttttat ttacggcttg agcctggctg
     1801 agctcgcaaa gcgtgcgaag tcaaaaaa
//
LOCUS       DAOSYE010000497         1822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54667_length_1822_cov_2.604414, whole genome shotgun sequence.
ACCESSION   DAOSYE010000497 DAOSYE010000000
VERSION     DAOSYE010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54667_length_1822_cov_2.604414"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLU43_08520"
     CDS             complement(<1..299)
                     /locus_tag="PLU43_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28495.1"
                     /translation="MAAENSSERKILAIIPARSGSKSVTDKNIRLMDGKPMLAYSIGH
                     AVRSKYINRVIVSTDSEKYAKIAKEYGAEIPFLRPAEYATDTALDFDVFFHALTFL"
     gene            complement(325..729)
                     /locus_tag="PLU43_08525"
                     /pseudo
     CDS             complement(325..729)
                     /locus_tag="PLU43_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
     gene            complement(719..1567)
                     /locus_tag="PLU43_08530"
     CDS             complement(719..1567)
                     /locus_tag="PLU43_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO28496.1"
                     /translation="MKKKRTFIQLLATALTNGYVLGFTKGKIYQGDLKKICVPGLNCY
                     SCPGALGSCPIGSLQAVLGSRNYRFSFYMLGFFMLVGALLGRAVCGFLCPFGLLQDLL
                     YKIPFFKKVKNLPGHKYLIYLKYVILVVFVILMPLIFVDIIGQGSPWFCKLICPAGTL
                     EGGWTLALLSPEIRESIGWLFTWKSFILIGILILSMGVYRPFCKYVCPLGGIYGLFNP
                     IAFYRISIDEEKCTKCSVCRKACPMDIPVYETPNSRECIRCGECKAACPHQAIKIGFG
                     GGRNDK"
     gene            complement(1581..1715)
                     /locus_tag="PLU43_08535"
     CDS             complement(1581..1715)
                     /locus_tag="PLU43_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HOO28497.1"
                     /translation="MKKYKSLFIAAAGIGLMIVGIYRQEVVVLYEKAIHICLECVGIG
                     "
BASE COUNT          490 a          502 c          331 g          499 t
ORIGIN      
        1 agaaacgtca gggcatgaaa aaaaacatca aaatcaagcg cagtgtccgt cgcatattct
       61 gccggacgca gaaacggaat ctccgcgccg tattcttttg ctattttggc atatttttca
      121 ctgtccgtac tgacaatgac cctgttgata tattttgatc tgaccgcatg cccgatcgaa
      181 tatgcaagca tgggtttccc gtccatcaga cggatatttt tatccgtaac actttttgaa
      241 ccgcttcttg caggtatgat tgcaagtatc tttctttctg aactgttttc tgctgccatg
      301 agcttcctcc gaacttttat tttcaaaaag acggtcatcg atatcgacca gtgtaattcc
      361 cgccagatac tccatacaat gcctgttcat ttccccgtaa ataccgtcca ttactcctgc
      421 cataccggat gcgatcaggc agtccatatt aggatcccct gttcttttcg atacggtgat
      481 cggcagctct cctaccgcgc tgcagacccg gtcaagtgta acagctgccg gcctttcaag
      541 aaaataacag ccgccgtcaa ttccttcctt tgtctcgatc aatgccgctt ttttcaattt
      601 tgcaagcact tttcgtacac gcgcaggatt ggtgcatacg ttttccgcta ttttctcact
      661 gctgacacag cactgcctgt gcttcataaa aaccagtgca tgtactgcta tcgcaaactc
      721 acttgtcatt acgtcctccc ccaaatccga tcttgatcgc ctgatgcgga caagccgcct
      781 tgcattctcc gcatcggatg cattccctgc tgttcggtgt ttcatatacc ggaatatcca
      841 tcggacatgc cttcctgcat accgagcatt tcgtacattt ttcctcatcg atcgatatcc
      901 tgtaaaaagc aatcggatta aacaacccat aaataccgcc aagcggacat acatacttgc
      961 agaacgggcg ataaacaccc atggacaaaa taaggatccc gatcagaata aagcttttcc
     1021 atgtaaaaag ccagccgatc gattctctga tttccggact taacagggca agcgtccagc
     1081 ctccctccaa agtacccgcc gggcagatca gcttacagaa ccacggagat ccctgcccga
     1141 taatatccac aaaaataagc ggcatcaaaa tcacaaaaac gaccagaatc acatatttta
     1201 aatagatcag atatttatgc ccgggaagat tctttacttt tttaaaaaat gggattttat
     1261 aaagcagatc ctggagcagg ccaaacggac ataaaaatcc gcataccgct ctgccgagca
     1321 gcgcgccgac cagcataaaa aacccgagca tataaaacga aaacctgtaa tttctgcttc
     1381 cgagaaccgc ctgcagggaa ccgatcggac aggatccgag tgctcccgga caggaatagc
     1441 agttaagccc gggaacacag atctttttca gatctccctg atatattttc ccttttgtaa
     1501 accctaaaac atatccgttc gtcaaagctg ttgcaagcag ctgtatgaac gtacgctttt
     1561 ttttcataag cagctcccaa tcagccgatc cctacacact ccagacagat atggatggct
     1621 ttttcatata aaacgacaac ctcctgtctg tatatgccga ctatcatcaa tcctatgcct
     1681 gccgcggcaa taaacagcga tttatatttt ttcatattga acgctccttt tcccggccgg
     1741 cgtgctttac tgaagtttct gtttctcttc ttctatcatc gcttcccagt cttccttatc
     1801 tcttgcccca acaacaatat ca
//
LOCUS       DAOSYE010000498         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54773_length_1820_cov_2.613598, whole genome shotgun sequence.
ACCESSION   DAOSYE010000498 DAOSYE010000000
VERSION     DAOSYE010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54773_length_1820_cov_2.613598"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLU43_08540"
     CDS             complement(<1..605)
                     /locus_tag="PLU43_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28498.1"
                     /translation="MEKRVALAVRILSPIVAVTAVIFLVIATMSNRADTTFLNDPYQM
                     LDSGWQCTIGDQPPIAIEPPMNIANIMDDDTYSFRLSRKLPDEIPFGYELGFRLSLQS
                     VRVFVDGEEVYEYGCDTDYPLWRSAGSLYVFVPLLPAYAGKTIEIELSSPYTENKGVI
                     NEFYFGKRIAIIQEIKRLFAVRNSIATLFVCVGCLLIAVYF"
     gene            complement(630..1610)
                     /locus_tag="PLU43_08545"
     CDS             complement(630..1610)
                     /locus_tag="PLU43_08545"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO28499.1"
                     /translation="MALKQEVQRILSENQGSSISGERMAEQLSVSRNAIWKAIKALQA
                     EGVEILAVTNKGYCLSEKNDILTVSGIKSHLKRYNEKLTIEIRDLVSSTNTVLKEMAE
                     NGMPEGHILIAKEQTAGKGRKGRKFFSPKDGSLYMSVLLRPQIRMEEALFITTAAAVS
                     VARAVEDTSGKVPAIKWVNDIFLNEKKICGILTEASVNMETGFPAYAVLGIGVNIYPP
                     RDGMPKELGPIAGTVFNSFYEGGNNYGNRLAAEILNYFFEYYENLTQKTFMGEYQRRS
                     FLTGRTVTYVSGNVKRRITVLGIDDNAGLIAETEDGERIILDSGEVSVKL"
     gene            complement(1622..1804)
                     /locus_tag="PLU43_08550"
     CDS             complement(1622..1804)
                     /locus_tag="PLU43_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6472 family protein"
                     /protein_id="HOO28500.1"
                     /translation="MSADVNCESCGNYLYDEEFEEYYCQMNLDEDEYGRILSDSNYRC
                     PYFQFNDEYKVVRKQI"
BASE COUNT          483 a          509 c          283 g          545 t
ORIGIN      
        1 ataaaataaa cagctatcag cagacatcca acacagacga acaaagtcgc aatactgttt
       61 ctgacagcaa acagacgttt gatctcctgt atgatcgcga tcctttttcc aaaataaaat
      121 tcattgatca cccctttatt ttccgtataa ggagaagaca attctatttc gatcgttttc
      181 ccggcatatg ccggcagcaa cggaacaaat acgtaaaggc ttccggccga acgccataac
      241 ggataatccg tatcacagcc atattcataa acttcctctc cgtctacaaa aacacgaaca
      301 ctttgtaagg aaagacgaaa tccaagttca tacccaaacg gaatttcatc cggcagtttc
      361 ctcgacagtc tgaaactata cgtatcgtca tccattatat tcgcgatatt catcggcggc
      421 tcaattgcga tcggcggctg atccccaatc gtacactgcc atccgctgtc cagcatctga
      481 tacggatcat ttaaaaaagt agtatctgcc cgattagaca ttgtagcaat aaccaaaaaa
      541 atcaccgctg tcacagcaac gatcggtgat aatatccgaa cagccaaagc aaccctcttc
      601 tccatccatt ccctccttcc tcttctgtct caaagcttaa cactcacttc tcccgaatcc
      661 agtatgattc tttcaccatc ctctgtttcc gcaatcagcc ctgcattatc gtcaatcccg
      721 agcactgtta tccgtctctt tacattaccg ctgacataag taaccgttct gcccgtcaaa
      781 aacgaacgcc tctgatattc ccccataaat gttttttgtg taagattttc gtaatactca
      841 aaaaaataat tcagtatttc agctgccagc cggtttccat aattatttcc cccttcgtaa
      901 aaggagttaa aaaccgttcc cgcaattggt ccaagctctt ttggcatccc gtcccttggc
      961 ggatagatat tgactccaat gccaagcact gcataggccg gaaaacccgt ttccatattc
     1021 acagaagcct ctgtcagtat tccgcatatt tttttttcgt ttaaaaatat atcgtttacc
     1081 catttgatcg caggcacttt tccggatgta tcctcaaccg cacgcgcaac ggaaaccgct
     1141 gccgccgttg tgataaacag tgcctcttcc attctgatct gcggccgcag cagtacgctc
     1201 atataaagac ttccgtcttt gggtgaaaaa aattttcttc cttttcttcc ctttcctgcg
     1261 gtctgttcct ttgcaatcag tatatgccct tccggcatcc cgttttctgc catttccttt
     1321 agtacggtat tcgtcgaaga tactaaatca cgaatctcta ttgtcagttt ttcattatac
     1381 ctcttcaaat gacttttaat ccccgatacg gtcagaatgt cattcttttc agacagacaa
     1441 tatcccttgt tcgttacggc cagtatttcg actccctccg cctgcagcgc cttgatcgcc
     1501 ttccatatgg catttctgct tacggaaagc tgttcagcca tcctttcgcc cgaaatactg
     1561 gaaccctgat tttctgataa tatccgctgt acctcctgct taagcgccat tttccaatct
     1621 cctatatctg ttttctgacc accttgtact catcgttaaa ttgaaaatac ggacaacgat
     1681 aattagagtc actcaaaatc cttccgtatt catcctcatc cagattcatc tggcagtaat
     1741 attcctcaaa ttcttcatca tacaaatagt tcccgcatga ttcgcaattg acatcagcac
     1801 tcatgtcctg ttccttcctc
//
LOCUS       DAOSYE010000499         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_54918_length_1817_cov_2.319523, whole genome shotgun sequence.
ACCESSION   DAOSYE010000499 DAOSYE010000000
VERSION     DAOSYE010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54918_length_1817_cov_2.319523"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PLU43_08555"
     CDS             complement(<1..1301)
                     /locus_tag="PLU43_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOO28501.1"
                     /translation="MRTLALPDNVLLNIEKPARYIGNEFNAVIKDKNEISIRFCMCFP
                     DLYEIGMSHLGIQILYDMFNQREDTWCERVYSPWTDLHKIMKEQRIPLFALESQDPVK
                     EFDFLGITLQYEMCYTNVLQILDLSQIPLYAKNRTEDDPIVIGGGPCSYNPEPIADFF
                     DLFYIGEGEQAYALLLDTYKEYKNAGKSRMEFLRAAAKLPGIYAPMFYEVVYHNDKTI
                     ASFLPAEKDIPAIIQKEIIMDITNTYYPKKPVVPFIKVTQDRVVLEIQRGCIRGCRFC
                     QAGMIYRPVRERNVEMLKAYAESMLKNTGHEEISLSSLSSSDYSDLKELVDFLMSHAE
                     NHKLNISLPSLRIDAFSLDIMKKIQDVRKSSLTFAPEAGSQRLRDVINKGLTEEIILE
                     GAKNAFLGGWTRVKLYFMLGLPTETEEDICKIAELSNEIAKL"
     gene            complement(1428..>1817)
                     /locus_tag="PLU43_08560"
     CDS             complement(1428..>1817)
                     /locus_tag="PLU43_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOO28502.1"
                     /translation="GVTCVVRWYNGWTDELWKTEVVAYGTTLPDPADPCLDGFEFVGW
                     EGSWTNITEDRAIKAWYYRRYKVKFINSETGEVYDTQAIRSGGDAEYPSTPSMDGYSF
                     DHWEGSRKYSGGYLNICSIYEKLTAYK"
BASE COUNT          496 a          419 c          324 g          578 t
ORIGIN      
        1 agtttagcga tttcattgga aagctctgca attttacaga tatcttcttc tgtttctgtt
       61 ggaagtccaa gcataaaata gagctttact cttgtccagc cgcctaggaa tgcattttta
      121 gcaccttcca gaataatttc ttccgtcagc cctttattaa tgacgtctcg cagcctttga
      181 gaaccggctt ccggtgcaaa ggtcaggctg ctttttctga catcctgaat ttttttcata
      241 atatctaaag aaaatgcatc aattctgagc gacggcagag aaatattcag tttatgattt
      301 tctgcatgtg acattaagaa atcaacgagt tcttttaaat ccgaataatc gctggagctt
      361 aaagaactta aggaaatctc ttcatgaccg gtatttttta gcatgctttc agcatacgcc
      421 ttaagcattt ccacatttcg ttccctgacc ggtctgtaga tcataccagc ctgacaaaac
      481 cggcagcctc tgatacatcc ccgctggatt tccagaacga ctctgtcctg tgttactttg
      541 ataaacggca cgaccggttt tttaggataa taggtatttg taatatccat tatgatctct
      601 ttttggatta ttgccggaat gtctttttct gcaggcaaaa aagatgcaat tgttttatcg
      661 ttatgataga ccacctcata aaacattggt gcatagattc ccggcagctt tgccgccgcc
      721 cgtaaaaatt ccatcctgga ttttcctgcg tttttgtatt ccttataagt gtccagaaga
      781 agtgcgtatg cctgctctcc ttccccaata taaaacaaat caaaaaaatc agcgatcggc
      841 tcaggattat aagagcaggg acctccgccg atcacgatcg ggtcatcctc cgttctgttt
      901 ttggcataaa gcggaatttg agacagatcc agtatctgca gtacatttgt ataacacatc
      961 tcatactgca gggtaatccc taaaaaatca aattctttta cgggatcctg tgattccagc
     1021 gcaaacagag gaattctttg ttccttcatg attttatgaa gatccgtcca cggagaatag
     1081 acacgttcgc accacgtgtc ttctctttga ttgaacatat catacaggat ctgaataccc
     1141 agatgagaca tgccgatctc gtacagatcc ggaaagcaca tgcagaaccg gatggaaatt
     1201 tcatttttat cttttattac cgcattgaat tcattaccga tatatcttgc cggtttttca
     1261 atatttaaaa gcacgttgtc aggtagtgca agtgttctca tactggctgc ctttctgtca
     1321 caattaacaa atcatttaaa gtatacgtga aactatttat aaaatcaaat acattatctg
     1381 gtattttccg tacataaaac aagacgtatt ttcatacgtc ctgtctttta tttatatgct
     1441 gtcaattttt cgtaaatact gcatatattt aagtatccgc ctgaatattt tctggaacct
     1501 tcccagtgat caaaggaata tccatccatg gacggagtcg acggatattc tgcatctcca
     1561 ccgcttcgaa tcgcctgggt gtcatatact tctccagttt cgctgttgat aaattttacc
     1621 ttatatctgc ggtaatacca agccttgatc gcacggtctt ccgtaatatt cgtccaggaa
     1681 ccctcccagc cgacaaattc gaatccgtca agacatggat ccgcaggatc cggcagcgtc
     1741 gtcccatatg ctacgacttc tgttttccat aactcatctg tccatccatt ataccatctg
     1801 actacacagg ttacacc
//
LOCUS       DAOSYE010000500         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55050_length_1814_cov_3.150085, whole genome shotgun sequence.
ACCESSION   DAOSYE010000500 DAOSYE010000000
VERSION     DAOSYE010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55050_length_1814_cov_3.150085"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..374
                     /gene="rhaM"
                     /locus_tag="PLU43_08565"
     CDS             63..374
                     /gene="rhaM"
                     /locus_tag="PLU43_08565"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOO28503.1"
                     /translation="MVKGFKMKLYPGMEAEYEKRHNELWQEMKDMIHAHGGRNYTIFL
                     DRETLTLFGYIEIKDEELWAQGADTAINRKWWDFMADIMETNSDNSPISIDLQPLFHL
                     T"
     gene            611..>1814
                     /locus_tag="PLU43_08570"
     CDS             611..>1814
                     /locus_tag="PLU43_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO28504.1"
                     /translation="MSKWVYLFKEGNADMRNLLGGKGANLAEMTNLGLPIPQGFTVTT
                     EACTDYYNSGKQISDEIKTQIFDALEILEEQQGKKFGDLENPLLVSVRSGARASMPGM
                     MDTILNLGLNDVSVEGFANKTGNARFAYDSYRRFIQMFSDVVMEMSKKFFEGILDEIK
                     EAKGVQYDTELTADDLKEVIARYKSVYQEKMGTEFPQEPKVQLMEAVKAVFRSWDNPR
                     AIVYRRMNDIPGDWGTAVSVQAMVFGNMGNTSGTGVAFTRNPSTGEKGIYGEYLINAQ
                     GEDVVAGIRTPQPITRLAQDMPSVFEQFMEIANKLEAHYRDMQDMEFTIENEKLYFLQ
                     TRNGKRTAPAALQIACDLVDEGKITPKEAVLRIEAKSLDQLLHPTFDTRALKEGQVIG
                     EALPASPGA"
BASE COUNT          568 a          308 c          491 g          447 t
ORIGIN      
        1 tctctcttat agaatgaagt ttatagaaga gaaaatgata aaaataaaag gaatgataaa
       61 acatggtaaa agggtttaag atgaagcttt atccagggat ggaagccgaa tatgaaaaac
      121 gccataatga gttatggcag gaaatgaagg atatgatcca tgcacacggc ggcagaaatt
      181 atacgatatt tctggacagg gaaaccttga cgttatttgg gtatatcgag attaaagacg
      241 aggaattgtg ggcacaggga gctgatacgg ccatcaatcg gaagtggtgg gatttcatgg
      301 cggatatcat ggaaacaaat tcggataaca gtccgatcag cattgatctg cagccgttat
      361 ttcatcttac gtgattcaaa ttgcccgatt tgtatgattt gtacaattta ttttacaaaa
      421 acaacaaaca acatcattat aaatacttta atggtgttgt ttttctgatt atgaatattg
      481 tcaaaacaat atcaaacaaa aataaatgtt gaattgtgtt gttttttact ggaaaaatat
      541 gaaataatgt gatatagtaa atttacgagc agttgaactt gtaactagta caaatttgga
      601 ggaaaagtga atgagtaaat gggtgtattt attcaaagaa ggtaatgcgg atatgaggaa
      661 ccttttagga ggaaagggag cgaatctggc ggaaatgaca aatctgggcc ttccgattcc
      721 gcaggggttt acggtgacga cggaagcctg cacggattat tacaacagcg gaaagcagat
      781 cagcgatgag atcaaaacgc agatatttga tgcgctggag attctggaag aacagcaggg
      841 caagaaattc ggggatcttg agaaccctct gcttgtatct gtcagatcag gagcgcgtgc
      901 atcaatgccg ggcatgatgg atacgattct gaatctcgga ctgaatgatg tttcggtaga
      961 gggctttgca aacaagacgg gaaatgcgag atttgcctat gattcctacc ggcgttttat
     1021 tcagatgttt tcagacgttg taatggaaat gagcaagaaa ttttttgaag gaatactgga
     1081 tgagatcaaa gaagcgaaag gcgttcagta tgatacggag ctgacggccg acgatctgaa
     1141 ggaagtgatc gcaagatata aatcggttta tcaggaaaag atgggaaccg aatttccgca
     1201 ggagccaaag gtgcagctga tggaagcggt caaggcagta ttccgttcct gggataatcc
     1261 gcgcgcgatc gtttacagaa gaatgaacga cattccggga gactggggaa ctgcggtcag
     1321 tgtccaggca atggtatttg gcaatatggg aaatacatcc ggaacaggcg tggcatttac
     1381 acgtaatccg agcacaggtg aaaaaggaat ttacggagaa tatctgatca atgcacaggg
     1441 tgaagacgtt gtggcaggta tccgtacgcc gcagccgatc acccggcttg cacaggatat
     1501 gccgtctgtg ttcgagcagt ttatggagat cgcgaacaag cttgaagctc attacaggga
     1561 tatgcaggat atggagttta cgatcgagaa tgaaaagctg tacttcctgc agacaagaaa
     1621 cggcaagcgt acggctcctg ctgctttgca gatcgcctgt gatcttgtgg atgaaggaaa
     1681 gatcacaccc aaagaggctg ttttacggat cgaggcaaag tcgctcgatc agttgctgca
     1741 tccgaccttt gatacccggg cgctgaagga agggcaggtc attggtgaag cgctgccggc
     1801 atctccggga gcgg
//
LOCUS       DAOSYE010000501         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55232_length_1810_cov_3.315100, whole genome shotgun sequence.
ACCESSION   DAOSYE010000501 DAOSYE010000000
VERSION     DAOSYE010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55232_length_1810_cov_3.315100"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PLU43_08575"
     CDS             complement(<1..1723)
                     /locus_tag="PLU43_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO28505.1"
                     /translation="MKLRTKIIMLSTIPIVVLGIFTYIFASVSIKKNMYNEIYTGLHA
                     TAISVRNQLDSRTKGEYKVDDEGKYMWKGSTYNITTDTDLVDTVKEETGIVTTVFYGD
                     TRYATSVTKDDGDRALYTQAGEKVVEEVITNGNEYFADNVDVVGTLYFTCYIPLLQEH
                     SSTPIGMVFCGKNQAVVDAEINKVTNSILGVTALVVLLCCAVAIIMSNRIVKVMEKGV
                     HMIDEVSKGNLSIELEPKYMNRKDEIGNICRYVEKLRQELITIVGGLQTQSGTLNESS
                     ARLEMTASNASEAVAQVERAIHEIADGAASQADETQKATENVILMGNMVEETGIQVSD
                     LKKNAEEMQKSSDEAYAILTELNQINQKAIGAIDVIYEQTNTTNESAMKIREATNLIT
                     EIADETNLLSLNASIEAARAGEQGRGFAVVAAQIQKLAEQSNESARQIEEIITSLIYD
                     STKSVETMNEVRAIMEQQNKNVQKTESAFSVVKDGIDRSMEGVGFIAGKTKNLDEARV
                     NVVDVVQNLTAIAEENAAGTEETSASSMEFGNMVKNIKQETDALKNVADEIEERMKQQ
                     VNKNVLFL"
BASE COUNT          463 a          450 c          287 g          610 t
ORIGIN      
        1 taagaaaaag tacattttta ttcacttgtt gcttcatgcg ttcttcaatt tcatctgcaa
       61 catttttaag cgcatccgtc tcctgtttaa tatttttgac catgttccca aattccatag
      121 aagaagcgga tgtctcttcc gtaccggcag cattttcttc tgcgatcgcg gtcagattct
      181 gtacaacatc tacaacattt actctcgcct catcaagatt ctttgtttta ccggctataa
      241 aaccaacacc ttccatcgaa cgatcgattc catctttcac aacagaaaaa gcactttctg
      301 ttttttgaac atttttattc tgctgttcca taatagcacg tacttcattc attgtctcaa
      361 cagatttcgt ggaatcataa atgagtgaag taatgatttc ctcgatctgt cttgccgatt
      421 catttgactg ttccgccagc ttctgaatct gcgccgcaac aactgcaaat ccgcgtccct
      481 gttcacccgc acgggcagcc tcaatggaag catttaatga aagcagattc gtttcatcag
      541 cgatttccgt aatcagattt gtagcttctc tgatcttcat tgcggattca ttggtcgtat
      601 tcgtctgttc gtaaattaca tcgatcgctc caattgcttt ctgattgatc tgatttaatt
      661 ctgtcagtat ggcatatgct tcatcgcttg atttctgcat ttcctccgca tttttcttta
      721 agtcggatac ctgtataccg gtttcctcga ccatattgcc cataaggata acattttcgg
      781 tagccttctg cgtctcgtca gcctgtgaag cagcaccatc cgcaatttca tgaattgccc
      841 tctccacctg tgccactgct tcactcgcat tggatgccgt catttcaaga cgagcggaag
      901 attcattcaa tgttccgctt tgtgtctgca gtccgcctac gatcgtaatc aactcctgcc
      961 gcaatttctc aacatagcgg catatattcc cgatctcatc ttttctgttc atatatttgg
     1021 gttccagttc aatgctcaga ttgccctttg atacttcgtc aatcatatgc acgccctttt
     1081 ccataacctt tacgatcctg ttcgacatta ttatggcaac agcacagcat aataacacaa
     1141 ctaaagcggt cacgcccaaa atactatttg tcactttatt gatctccgca tcaacaaccg
     1201 cctgattttt tccgcagaag accatgccga tcggtgtact ggagtgttcc tgcagcagcg
     1261 gaatataaca cgtaaaatac agggtcccaa caacatcaac attatctgca aagtattcat
     1321 tgccatttgt aataacttcc tcgacaactt tttctccggc ctgtgtatac aatgctctgt
     1381 caccatcatc ctttgtgact gacgtcgcat atctcgtatc tccataaaaa accgtagtaa
     1441 caattccggt ctcttccttg accgtatcta ccagatcggt atcggtcgta atattataag
     1501 tagaaccctt ccacatatat ttgccttcat catctacttt atattcacct tttgttctgg
     1561 aatccaattg atttctgaca gatatggctg ttgcatgcag acctgtatat atctcattat
     1621 acatattctt tttaatgctg acagatgcaa aaatataagt aaatatacct agcacaacga
     1681 tcggaatggt ggataacata ataattttgg ttcttaattt catgttctgt tcccctcttt
     1741 ctgatttgac tgaaatatat tactcaaact tcgcacatag attttagcta tgcaccttga
     1801 aaattcaata
//
LOCUS       DAOSYE010000502         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55293_length_1809_cov_2.836374, whole genome shotgun sequence.
ACCESSION   DAOSYE010000502 DAOSYE010000000
VERSION     DAOSYE010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55293_length_1809_cov_2.836374"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PLU43_08580"
     CDS             <1..137
                     /locus_tag="PLU43_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOO28506.1"
                     /translation="KTLQYLMGHSDIATTLGIYTHIKYEDAEKEIRELEEKNRKICGE
                     "
     gene            328..786
                     /locus_tag="PLU43_08585"
     CDS             328..786
                     /locus_tag="PLU43_08585"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOO28507.1"
                     /translation="MISILFICHGNICRSPMAEFMMKDMVKKAGREEEFLIASAATSA
                     EEIWNGVGNPVYPPARRKLAQHGISCAGKKAVQLTRADYEKYDYLLGMDGRNISNMMR
                     LFGSDPEGKVFRLLDFSDDPRDIADPWYTGDFDTTYNDLLEGCRAFLKTV"
     gene            complement(773..1522)
                     /locus_tag="PLU43_08590"
     CDS             complement(773..1522)
                     /locus_tag="PLU43_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28508.1"
                     /translation="MKKWKAEILPDTKDPIYEQRETGTTHTPFRLEYVLYQSIMQGNK
                     KQMILAIEEYLQNGLVIGRLSKNAGNQTKYWAVSCIATAIHYAILGGLDETDAYNLSD
                     QYIRHIDRLTELEACIEYLEACAIELVEKVADAKTKEIHSPAIKQCIHYIHIHLHEKL
                     VIEALAKEVGLSRDYLSVLFKSETGTCLHAYILREKLEASKGMLSQGLDYDTIAYNLA
                     FCSESHYISGFKKEYGITPGEYRRTHPVIRS"
BASE COUNT          517 a          336 c          447 g          509 t
ORIGIN      
        1 caaaaacatt acagtacttg atggggcact ctgatatagc gacaactttg ggaatatata
       61 cgcatataaa atacgaggat gctgaaaaag aaataagaga actggaagaa aaaaacagga
      121 aaatatgtgg agagtaaaat agacctgtac ctttatggta ggtaaaattt ttggatttta
      181 cctaccattt tacttatttt gatggctaag atataccgag atatgctatg atattccaag
      241 aaacggttga aatgataata taaaaatagt ggctttaaac attgtaaata cagggttttg
      301 ccacgatatg aaaggatata ccaagatatg atttctatac tttttatttg ccacggcaat
      361 atttgtcgat ccccaatggc ggaatttatg atgaaggata tggtgaaaaa agcggggaga
      421 gaagaggagt ttttgatagc gtctgcggcg acttcagcag aggagatttg gaatggtgtg
      481 ggaaatccgg tatatccgcc ggcgcggagg aagcttgcgc agcatggaat ttcctgtgca
      541 gggaaaaagg ccgtgcagct gacacgcgcg gattatgaga agtatgatta tctgcttgga
      601 atggatggca ggaatatttc caacatgatg cggctgtttg gttcggatcc ggaaggaaag
      661 gtcttcaggc tgctggattt ttcggatgat ccaagggata ttgcagatcc gtggtatacc
      721 ggagattttg atacgacata taacgatctt ctggaaggct gccgggcgtt tcttaagacc
      781 gtatgacagg atgagtccgg cggtattctc cgggggtgat gccgtattcc tttttaaagc
      841 cggaaatata gtgagattcc gaacagaaag ccagattgta ggcgatcgtg tcatagtcca
      901 gcccttggga aagcatgccc tttgaggctt ccagtttttc gcgcaggata taggcatgga
      961 ggcaggtgcc ggtctcggat ttgaataata cggacagata atcccgtgac aggccgactt
     1021 cctttgcaag tgcttcaatg accagctttt catgcaaatg aatgtggatg taatgaatac
     1081 actgtttgat tgccggggaa tggatctcct ttgttttggc gtcagctact ttttcgacca
     1141 gttctatggc acatgcttcc agatattcga tacatgcttc cagctctgtg agccggtcta
     1201 tgtgacggat atattggtca ctcaggttat aggcatctgt ctcatccagt ccgccgagta
     1261 tggcgtaatg gatcgcagtc gcgatgcatg agacagccca gtatttcgtc tggttgccgg
     1321 cgttttttga aaggcggcca atgacaagtc cgttttgcag atattcttca atcgcaagaa
     1381 tcatctgctt tttatttccc tgcataatag attgataaag cacatattcc agccggaacg
     1441 gcgtatgcgt cgttccggtt tcccgttgtt catagatcgg atcttttgtg tccggcagaa
     1501 tctcggcttt ccattttttc atcagcagtc tccttattga caagtcataa atttgatttc
     1561 agaataacac ataaaagtga aatttaacac ataaaaatga tattcatctc atttttacga
     1621 tgttatattt gaattataaa aaagtcacga acaggaggat ccaacgcaca aatggaaaat
     1681 gctaaagcgg agctgacttc ttttgaaaaa cacaggcttg taacagggca gggaacgtgg
     1741 catacgtatg actgcaacca aaaactgccg tcacttcgta tgtcggacgg cccgcatgga
     1801 ttgagaaaa
//
LOCUS       DAOSYE010000503         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55327_length_1808_cov_3.478608, whole genome shotgun sequence.
ACCESSION   DAOSYE010000503 DAOSYE010000000
VERSION     DAOSYE010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55327_length_1808_cov_3.478608"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1204
                     /locus_tag="PLU43_08595"
     CDS             200..1204
                     /locus_tag="PLU43_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO28509.1"
                     /translation="MAKQVTMKDIANALHISTVSVSKALSGKEGVSIEVRERIRITAE
                     EMGYRYNAGVKAIEEDNNYNVGIIIARRFLEDDTAFYSKLHSNIIMKLGNKNSFGIME
                     VIFKEDEENCTLPAMIANKKVDGIIVLGQLKKAYLHMIMEQYLPITFLDFYDENSSVD
                     SIVSDNVYGSYLLTNYLIKCGHKKIGFIGDIFATSSILDRYLGYYKSLLQNEIPIKEE
                     WLLNDRDENGILRAITFPKKMPTAFVCNCDEVAYRVVEQLKAEGYRIPEDVSIVGFDD
                     YIYATLSNPALTTYAIDLQLMTEMAVESILKGISGENLSPGRKVISGKLIVRNSVAKL
                     "
     gene            1242..>1808
                     /locus_tag="PLU43_08600"
     CDS             1242..>1808
                     /locus_tag="PLU43_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOO28510.1"
                     /translation="MKELLFLEPIVKEMIWGKEYWTVSAHPSGDGTIKNGTYAGRKLS
                     WLWENKRELFGGAAGDQFPLLVKKIEAKEDLSIQVHPDDHYAKANENGSLGKTECWYI
                     IDCKPEGTIIIGHHANSREEAEKMIEQNEWKKLLRELPIKKGDFFQINPGTVHAIKND
                     TVIMEIQQSSDITYRLYDYGRLKDGSPRE"
BASE COUNT          616 a          292 c          439 g          461 t
ORIGIN      
        1 gtattttgaa acgtatcaga tagaagctgc atgcaataaa attatagacg aatgaacaaa
       61 cgagttaata aataagacgc ccctattttt ttaagtaaat ttactttacg gcttctttat
      121 aaagtgatat aataaccggg taacagattg tatggtaaag tgtttacgaa aataaaatat
      181 aaatgatggg gacaggaaaa tggcaaaaca ggtaacaatg aaggatattg caaatgcgct
      241 gcatatcagt acggtatcgg tatccaaagc attatccgga aaagaagggg tcagcatcga
      301 ggtccgggag cggatcagga ttacggcaga agaaatgggg tatcgctata atgccggagt
      361 gaaggcaatc gaagaagata ataattacaa tgtcggtatc attattgcaa gaagattttt
      421 ggaagatgat accgcattct attctaagct gcacagtaat attattatga aacttgggaa
      481 taaaaacagt tttggtatta tggaagtcat ttttaaagaa gacgaagaaa actgtacgct
      541 tcccgcaatg attgcaaata aaaaagtaga cggtatcata gtactcggtc agctgaaaaa
      601 agcatatctt catatgatca tggaacagta tcttccaatc acctttttag atttttatga
      661 tgaaaactca tccgtcgatt ccattgtcag cgataatgta tatggaagct atcttctgac
      721 aaactatctg atcaagtgcg gtcataaaaa gatcggattc atcggggata tttttgcaac
      781 aagcagcatt ctggaccgtt accttgggta ttataaatca ctgcttcaga atgaaattcc
      841 gataaaagaa gaatggctgc tgaatgacag ggatgaaaac ggaattctgc gggcaatcac
      901 gtttccgaaa aaaatgccga cagcctttgt atgcaattgt gacgaagtgg cctatcgtgt
      961 tgtggagcag ctgaaggcag aagggtatcg gattccggaa gatgtctcca ttgttggatt
     1021 tgatgattat atttatgcaa cgctttccaa tccggcattg acgacttatg cgatcgatct
     1081 gcagctgatg acggaaatgg ccgtggagtc catattaaag ggaatttcgg gagagaatct
     1141 ttctccgggc agaaaagtaa tcagcggaaa gctgattgta agaaattctg tggcaaagct
     1201 gtaaagatac tatattaaga aggaaaaggg atgtttaaat catgaaggaa ttactttttt
     1261 tagagccgat tgtaaaggaa atgatatggg gaaaggaata ttggaccgtc agtgcgcatc
     1321 cgagcgggga cggcacaata aaaaacggca cttatgcggg caggaagctt tcctggctgt
     1381 gggaaaataa aagagagctg tttggaggag cggcaggcga ccagtttccg cttcttgtaa
     1441 aaaaaatcga ggcaaaggag gatctgagta ttcaggtgca tccggatgat cactatgcaa
     1501 aggcaaacga gaacggttcg ctcgggaaaa cggaatgctg gtatattatc gactgtaagc
     1561 cggagggaac gattattatc ggccatcatg caaattccag agaagaagcc gaaaaaatga
     1621 tagaacaaaa tgaatggaaa aaactgcttc gtgagcttcc gatcaaaaaa ggagattttt
     1681 ttcagatcaa tccgggaacc gtacatgcca taaaaaatga tacggtcatt atggagatcc
     1741 agcaaagttc ggatattaca tatcgtctct atgattacgg cagattaaaa gacggcagtc
     1801 cgcgggag
//
LOCUS       DAOSYE010000504         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55607_length_1803_cov_2.412471, whole genome shotgun sequence.
ACCESSION   DAOSYE010000504 DAOSYE010000000
VERSION     DAOSYE010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55607_length_1803_cov_2.412471"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1286)
                     /locus_tag="PLU43_08605"
     CDS             complement(132..1286)
                     /locus_tag="PLU43_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28511.1"
                     /translation="MYTIMIVDDNETELVIAQKTLEKNYQIIPMNNSKQALARLAKAT
                     IMPDLMLLDIAMPGINGFEMIMRLKTSEKAKGIPVLFLSGDKENGTELEAYRLGAVDF
                     IRKPVVAELLRKRVELQINLLEYKKKMSTYNTQLQQTASYQAQNALRLEYFIIGIITD
                     LIARKDSYTGMHSIYASKHMGILLKEMLMSGVNYGIASEDYDLILLSSQLHDMGKIGV
                     PDSVLQKEGKYTKEEFELMKSHTIFAADAIQKYSYLLPNSKFLNYTYQMARYHHERFD
                     GNGYPDRLSGQNIPILAMILSVADVYDALISERSYREPLSHEQAYNIITQGAGVQFDP
                     QVVAAFQRVHMDLYEASQKMAMAVQQQGDLMSRQQMSLMPGNKEKNGKGK"
     gene            complement(1271..>1803)
                     /locus_tag="PLU43_08610"
     CDS             complement(1271..>1803)
                     /locus_tag="PLU43_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOO28512.1"
                     /translation="PGGLQIGGGICAENAGEFLDAGASHVIVTSYVFSDGKICYNHLE
                     KLKHEVGKGKIVLDVSCRIQDGCYYVVTDRWQKFTDTVFSKELLKELSGYCDEFLVHA
                     VDVEGKASGIEEEIVRLLAGSANPATYAGGISSCPDIEKIKKIGNDRVNVTIGSALSL
                     FGGQLDFEEVIRCIQL"
BASE COUNT          452 a          480 c          345 g          526 t
ORIGIN      
        1 ggaatgtaac agtgccgttt ccgtaccatt cagatgcgga gcagtgccgc ttgttgccgc
       61 aagtttttcg attgccgcac cggcgatcct gtccggaaat gtatgtgtat gaaaatcaat
      121 tatcatcgta attacttccc tttaccgttt ttttctttat ttcccggcat caaagacatc
      181 tgctgcctgc tcatcagatc tccctgctgc tgcaccgcca ttgccatttt ctgtgatgct
      241 tcataaagat ccatatgaac ccgctggaaa gccgccacta cctgcggatc gaactgaacg
      301 cctgccccct gtgtgataat attataagcc tgttcatgtg acagcggctc acggtaggaa
      361 cgctcggata tcaaagcatc gtatacatcg gccaccgaaa ggatcattgc cagtatcgga
      421 atattctgtc cggataatct gtccggataa ccattcccgt caaaacgttc atgatgatat
      481 cgtgccatct ggtatgtata attcaaaaac ttgctgttcg gaagaagata cgaatacttt
      541 tggatcgcat cagcggcaaa tatcgtatga ctcttcatca gctcaaattc ttcttttgta
      601 tattttcctt ccttctgcaa tacggaatcc ggcacaccga tcttccccat atcatggagc
      661 tgggaagata acaggatcag atcataatcc tcggaagcaa ttccgtaatt aacgccggac
      721 atcagcattt ccttcaaaag gatccccata tgcttggacg cataaatact gtgcattccc
      781 gtataactgt ctttcctggc gatcagatcc gtaatgatac caataataaa atattccagt
      841 ctcagtgcat tctgtgcctg atagctggcc gtctgctgca gctgggtatt ataagtgctc
      901 atcttctttt tgtactccaa aagattgatc tgcagttcca cgcgcttgcg cagaagctct
      961 gctacaacgg gttttctgat aaaatcaaca gccccaagcc tgtatgcttc cagctccgta
     1021 ccgttttcct tgtctcctga aagaaataaa accggaatcc cctttgcttt ttcactcgtc
     1081 ttcagccgca tgatcatctc aaaaccatta atgccgggca ttgcaatgtc aagcagcatc
     1141 agatccggca tgatcgttgc ttttgcaagc ctggccagcg cctgcttact gttattcatt
     1201 ggtatgatct gataattttt ttccaatgtt ttctgtgcga taacaagctc tgtctcatta
     1261 tcatctacga tcataattgt atacatcgga ttacctcctc aaaatcaagc tgtccgccga
     1321 acaagctcag cgcgcttcca attgtcacat tgactctgtc gtttccgatc tttttgattt
     1381 tttctatatc cggacaggac gagatcccgc ccgcataagt agccgggttt gcagagccgg
     1441 caagcagccg gacgatctct tcttcgattc cggacgcctt tccttccaca tccacggcat
     1501 gcaccagaaa ttcgtcacaa tatcccgaaa gctctttcag cagttctttt gaaaataccg
     1561 tatccgtaaa tttctgccat ctgtctgtta caacgtaata acagccgtct tgaatccggc
     1621 agctgacatc aagtacgatc ttcccttttc cgacctcatg tttaagcttt tcaagatgat
     1681 tatagcatat tttaccatca ctgaatacat aggaagtgac gatcacatga gaagcaccgg
     1741 catccagaaa ctcgccggca ttttccgcac agatacctcc tccgatctgc agaccgccag
     1801 gat
//
LOCUS       DAOSYE010000505         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55633_length_1802_cov_3.430452, whole genome shotgun sequence.
ACCESSION   DAOSYE010000505 DAOSYE010000000
VERSION     DAOSYE010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55633_length_1802_cov_3.430452"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1156
                     /locus_tag="PLU43_08615"
     CDS             101..1156
                     /locus_tag="PLU43_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOO28513.1"
                     /translation="MKKTKNSTFQRIINIKEWETNTKKFIGTIVVLFIVASAFACFKP
                     SKAELLSHASSDVETALKEDESKTRKEVVTTYNDLYFINYCSFETSGANALSEKFLGF
                     AGHVYPADQGVPKIAGQMVNYTYRMLGCGQSVLYGAKLTIFLTTLSVLCGFFLSIFLA
                     LGKISKIKPLSKFCSGYIFFFRGTPLMIQIFVIYLAIPGIIPGFNWRGLFSDNDPEAV
                     FKGAFVAAFIAFTLNTAAYCAEIVRAAIQSIDKGQFEASKALGMNYAQTMRLIVIPQS
                     FRRMIPPVCNEFVMVLKDASLVFVIGLMDITTISKTIASGGSFLVFIPALIIYLIITA
                     FFTFIFDKLEKRLSAYE"
     gene            1177..>1802
                     /locus_tag="PLU43_08620"
     CDS             1177..>1802
                     /locus_tag="PLU43_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOO28514.1"
                     /translation="MIRTENLCKNFGDLKILKNISMSVEQGEVVVIIGPSGAGKSTLL
                     RSLNHLEKATSGKIFIEGQMLDNREKEVNHTHLNKHEKNILLLKMGMVFQRFNLFPHK
                     TVLENVMIAPVNVKKMSNEQAEVIARDLITRVGLEDKMHVYPNKLSGGQQQRVAIARA
                     LAMQPEIMLFDEPTSALDPELVGEVLAVMQNLAKGGMTMICVTHEMGFA"
BASE COUNT          538 a          370 c          382 g          512 t
ORIGIN      
        1 cgatcgaaga gctgacggca gaaggcttct ttgaagaaaa cactgcaaaa tggtttggtg
       61 caggcgaata aaaatatcat aggaaaggct tgagattact atgaagaaaa cgaagaacag
      121 tacctttcag cgcataatca atataaaaga atgggaaaca aatacaaaaa aatttatcgg
      181 aacgatcgtt gttctcttta tagtggcctc tgcttttgca tgctttaagc cttcaaaagc
      241 agaattattg agccacgcct cttctgatgt ggaaacagcg ctcaaggaag atgaaagcaa
      301 aacacgaaaa gaagttgtca caacctataa tgatctttat tttatcaact actgttcttt
      361 tgaaacttca ggtgccaatg cacttagtga aaaattcctc gggtttgcgg gacatgtcta
      421 tcccgcagac cagggggttc ctaaaatcgc aggacagatg gtcaattata cttaccgcat
      481 gcttggctgc ggacaaagtg ttctgtacgg tgccaaactg accattttcc tgaccactct
      541 ctcagttctg tgcggtttct ttctcagtat ctttcttgcc cttggtaaaa tatcaaaaat
      601 taagccgcta agtaaattct gcagcggtta tatcttcttt ttcagaggca cccctttaat
      661 gattcagatt tttgttatat atcttgcaat tccaggaatc attcccggat ttaactggcg
      721 cggtctgttc agcgacaatg acccggaagc agtattcaag ggtgcttttg ttgcggcttt
      781 tattgctttt accctaaaca ctgctgctta ctgcgcggaa atcgttcgtg ctgccattca
      841 gtctattgac aaaggacaat ttgaggcaag taaagcgctt ggtatgaatt acgcacagac
      901 tatgcgtctg atcgtcatac cacaatcatt cagacgtatg atcccgcctg tttgtaatga
      961 attcgtcatg gtacttaaag acgcatcact cgtctttgtt atcggtctga tggatattac
     1021 tacaatttca aaaacaattg cttccggagg ctcgttcctt gtgtttatac cggccctgat
     1081 catttatctg atcatcaccg cattcttcac atttattttt gataaactgg aaaaacggct
     1141 ttcggcttac gaataggggg aaaagcagct atggaaatga ttcgtacgga aaatctttgt
     1201 aaaaattttg gtgatctgaa aattctaaaa aatatatcca tgtctgtgga acagggagaa
     1261 gtcgttgtta ttatcggccc atccggcgcc ggaaaatcca ctcttctccg ttctctgaat
     1321 catctggaaa aagcaacgtc cggaaagatc tttattgaag gccagatgct tgacaacaga
     1381 gaaaaggaag ttaatcatac gcatttgaac aaacacgaaa aaaacatcct tttattaaaa
     1441 atgggaatgg tcttccagag atttaatctc tttccgcaca aaacagtatt ggaaaatgtt
     1501 atgatcgcac ctgtcaatgt caaaaaaatg agcaatgaac aggcagaggt cattgcaaga
     1561 gacctgatca cacgtgtcgg tcttgaagat aaaatgcatg tatatccaaa caagctgtcc
     1621 ggcggacagc agcagcgtgt tgcaattgca agagcgcttg ccatgcagcc tgaaatcatg
     1681 ctgtttgacg agcctacatc agcccttgat cctgagcttg taggagaagt actggctgtt
     1741 atgcagaacc ttgcaaaagg cggtatgacg atgatctgtg taacccatga aatgggattt
     1801 gc
//
LOCUS       DAOSYE010000506         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_55700_length_1801_cov_2.888889, whole genome shotgun sequence.
ACCESSION   DAOSYE010000506 DAOSYE010000000
VERSION     DAOSYE010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55700_length_1801_cov_2.888889"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..688)
                     /locus_tag="PLU43_08625"
     CDS             complement(257..688)
                     /locus_tag="PLU43_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO28515.1"
                     /translation="MNYESLAQEYMELLHQLRRWDAKEHITASVHGERFILFYIYKKG
                     GSVIPSDISKEMGITSARVAAALNSLEGKGLISRRIDGEDRRRILVDLTEDGKRKICR
                     QHRKVMGAIINMLQYLGEDDAKELIRIMKKLLSMKRQNRQE"
     gene            1074..1238
                     /locus_tag="PLU43_08630"
     CDS             1074..1238
                     /locus_tag="PLU43_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28516.1"
                     /translation="MQNKNNYKEILSESLNACVGKRFPGAVLKQVLPHESGLHCDFDL
                     ERPINKSDFD"
     gene            1251..>1801
                     /locus_tag="PLU43_08635"
     CDS             1251..>1801
                     /locus_tag="PLU43_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO28517.1"
                     /translation="MEQACPGFSFELSGYSGVYEDGDAEKKVLQRISMIAFENQADFD
                     AYQAFLLQAKERDHKEIGAKQKLFLSSEDIGGGLILWGPKGAMIRHLIEQFGQNAHII
                     NGYDWVYSPHIGKAKLWETSGHLQFYKDSMYSAIDIDGEQYYLKPMNCPFHIKIYNSE
                     IRSYKDLPMRLAEYGSVYRYEMSG"
BASE COUNT          495 a          428 c          358 g          520 t
ORIGIN      
        1 agatttcgct tcctggtgtc tgcgtaagtg ttgtgatctt tgacatataa tccgggagct
       61 tcagatccag ccagacctgc agtacaataa acagcaggct gagcaatccc tgacgcagct
      121 ccgctgcctt taaacgtttt aataatcgaa tcattgcgtt cctccttttt attaatagca
      181 ttatatacta atactgttag ctatttgtga taataaatgc agccacacgc tgcgactgca
      241 tatgatcttt ataaattcat tcctgccggt tctgccgttt catcgacagc agctttttca
      301 tgatacgaat aagctctttg gcatcgtctt ctcccaaata ctgaagcata ttgatgatcg
      361 cacccatcac tttccgatgc tgtctgcata ttttccgctt tccgtcttct gtcagatcga
      421 ccagtattct cctgcggtct tcaccatcga tccttcttga gatcagacct ttcccttcca
      481 gactgttaag agcagccgca actctggctg aagtgattcc catctctttg ctgatatcgc
      541 ttgggatcac acttcctcct ttcttataaa tataaaaaag aataaatctt tccccatgta
      601 cggaagcagt aatatgctcc tttgcatccc atctgcgcaa ttggtgcaat aactccatat
      661 actcctgcgc caaactttcg taattcatgt tttcaccttt ccgcggaaat aactaacact
      721 attaactata taagtgccag atctgtttgt caatcccttt ttacagccct ttttattttt
      781 taaggattga cacattgcca catgctgtga tatcatcata cacaaatcaa ttttaaggag
      841 tgtttcctat gtttttaaag atccatcatg ttacagccac cacgaccacc aagagcttgc
      901 ttctgtagtc tgttgtaaat aaaattttac agccggccgc aggggaaagc tcctgtcggc
      961 gtggctgtaa agggatctat atcacaatca ccttttcaga taaccacgtt gtctgaaaag
     1021 gtgatttttt attacatgca aacattcaaa taatctgtta aaggagaaaa acaatgcaaa
     1081 acaaaaacaa ttacaaagaa attttatctg aaagtctgaa cgcctgtgtc gggaaacgct
     1141 ttccaggggc ggtcctaaaa caggtcctcc cccatgagtc cggtctgcac tgtgattttg
     1201 acctggaaag accaatcaat aagtctgatt ttgactagct gtgtgccgac atggaacaag
     1261 cctgtcccgg attctcattt gaactctccg gttattccgg ggtttacgaa gacggcgacg
     1321 cagagaaaaa agtactgcag cggatatcca tgatcgcttt tgaaaatcag gccgactttg
     1381 atgcttatca ggctttcctg ctgcaggcaa aagaacggga tcataaggaa atcggcgcaa
     1441 agcagaaact gttcttaagt tcggaggaca tcggcggcgg acttatttta tggggtccaa
     1501 aaggcgccat gatccgtcat ctgatcgaac agttcgggca gaatgcgcat atcatcaacg
     1561 gatatgactg ggtctattcc ccgcatatcg gaaaagcgaa gctgtgggaa acgtccggac
     1621 atctgcagtt ctacaaggac tccatgtaca gtgccatcga cattgacggc gaacagtact
     1681 atctgaaacc gatgaactgt ccgttccata taaaaatata taacagtgag atccgttctt
     1741 ataaagacct gccgatgcgt ctggcagaat acggctccgt ataccggtat gaaatgtccg
     1801 g
//
LOCUS       DAOSYE010000507         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_56683_length_1782_cov_2.470180, whole genome shotgun sequence.
ACCESSION   DAOSYE010000507 DAOSYE010000000
VERSION     DAOSYE010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56683_length_1782_cov_2.470180"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLU43_08640"
     CDS             complement(<1..931)
                     /locus_tag="PLU43_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28518.1"
                     /translation="MKKVLIVEDAKMIRKGISTMVGRCGIPIGEILECKNGLEALEII
                     KNNRVDVMITDIRMPKMDGITLVKELQKQPYIPKIIVISGYDDFSFAVDLLRCGAREY
                     LLKPIKREDLKAIMEKLEAELRKENAEKEGLREVCYGQIRALLSSNTKAGEKVDETGI
                     CDRIKNEAYQVICTNESAEGKCAEENFFFFLNIEGCNVILVPAEKTEQFVEKLVFRQF
                     IGISESYQGLQDLRNAFLDAFKKRERAFCMQDETASDDYRISLNELESFVQKIGTQRN
                     SENECYLNEIADKVKQGKMSAGRFQETIRQIVSR"
     gene            complement(909..>1782)
                     /locus_tag="PLU43_08645"
     CDS             complement(909..>1782)
                     /locus_tag="PLU43_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO28519.1"
                     /translation="SFNWFSLDYIYYPVSGIMWLWYKLFGAILGAENFFAWGLSVMFL
                     VFTLRALLYKPFVRQIETTRQMQELQPQIKALQNQINAHFIYNVLESVKMMAEINEEY
                     EIADAISSFGKLLRYGMRWKSQYVTVEEEIDYIKDYLALFNLRFDYEISLNLTLSPGI
                     MSQEIPKMSLQPIVENAVSHGMEEITEDSAIDINGEVEDDSCYIRIRDPGCGMSEEEV
                     DRLRKRLRGEIADISGSGNGIGLKNVQDRLQIRFGPEYGIKVTSKEGVYTQVEVKIPY
                     REVRRISDEKGSDC"
BASE COUNT          413 a          496 c          337 g          536 t
ORIGIN      
        1 cccggctcac gatctggcgg atcgtctcct gaaatctccc tgcactcatt tttccctgct
       61 tgactttatc agcaatttca ttcaaatagc attcgttttc actgtttctc tgagtcccga
      121 tcttctgcac aaagctttcc agttcattta gtgagatcct gtaatcatcc gatgctgttt
      181 catcctgcat acagaaggct ctctctcttt ttttgaaagc gtcaagaaac gcattccgca
      241 gatcctgtaa tccctgatag ctttcactga ttccgataaa ttgcctgaat accagctttt
      301 caacaaactg ctctgttttt tctgcaggaa caaggatcac attacagcct tcaatattca
      361 aaaaaaagaa aaaattctct tccgcgcatt ttccttcagc ggattcgttc gtacaaatga
      421 cctggtacgc ttcgtttttg atccggtcgc agatccctgt ttcatcaact ttttccccag
      481 ccttcgtatt ggaagacagc agcgccctga tctgcccata gcagacttcc cgcagaccct
      541 ctttctccgc attttccttt ctaagctctg cttcaagctt ctccatgatc gctttcaggt
      601 cttctctttt gataggcttc agcagatatt cccttgctcc gcatcttaac agatcaactg
      661 caaacgaaaa atcatcataa ccgctgatca caatgatctt ggggatatac ggctgcttct
      721 gcaattcttt tacaagcgtg atcccgtcca ttttgggcat ccggatgtct gtgatcataa
      781 catcgacccg gttattttta atgatctcca atgcttccag gccatttttg cattccagga
      841 tctctccgat cggtataccg cagcggccga ccatcgtact gatccctttt cggatcattt
      901 ttgcatcttc aacaatcaga acctttttca tccgatatcc tccttacctc tctatatggt
      961 attttgactt ccacctgtgt atagacacct tctttggagg tgactttaat tccgtactcc
     1021 ggaccgaatc tgatctgcag tctgtcctgt acgtttttga gtccgattcc atttccggat
     1081 ccggatatat ctgcaatttc cccgcgaagc cgcttccgca gccggtcgac ctcttcctcg
     1141 ctcattccgc agcccggatc cctgatcctg atataacagc tgtcgtcttc tacctcaccg
     1201 ttgatatcaa tggccgagtc ctcagtgatc tcttccattc catgggatac ggcattttcc
     1261 acgatcggct gcagcgacat tttcggtatt tcctgggaca tgatgccggg tgaaagcgta
     1321 aggttcagac tgatctcata gtcaaaccgc aaattgaaaa gtgccaaata atctttaata
     1381 taatcgatct cttcctcgac cgtcacatac tgtgatttcc atcgcattcc atatctaagc
     1441 agctttccga aagaagagat ggcatctgca atctcatatt cttcatttat ttccgccatc
     1501 atcttcacgg attccagcac attataaata aaatgtgcat tgatctgatt ctgcagtgcc
     1561 ttgatctggg gctgcagttc ctgcatctgc cgggtggtct cgatctgacg gacgaacggc
     1621 ttgtacagca gcgcgcggag ggtgaagacc aggaacatca ccgacaggcc ccaggcgaag
     1681 aagttctccg ccccgaggat cgcgccgaaa agcttgtacc agagccacat gatccccgag
     1741 accgggtaat agatgtagtc cagactgaac cagttaaaag ac
//
LOCUS       DAOSYE010000508         1781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_56710_length_1781_cov_3.913673, whole genome shotgun sequence.
ACCESSION   DAOSYE010000508 DAOSYE010000000
VERSION     DAOSYE010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56710_length_1781_cov_3.913673"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="PLU43_08650"
     CDS             complement(<1..1624)
                     /locus_tag="PLU43_08650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO28520.1"
                     /translation="MNEKKNEPVSKVEDLAETKERIEKEQGDETERIILSEQTPKVRL
                     HKGVKAQKVFKRIGYILKKLKVLIILLIIAAIAFFVYRKWKTNQEMEQAMEAGSQMET
                     ATIEKRDLTDSISTTGTVEANDTRTLTSAMSDIKITSVNAEVGDEVVEGDILVTFSDE
                     DINRTIADLQEDIAVSEQTDEIESEAQDRDYLYTYGTEANNLSSAAEKVTDALNDVYE
                     ACDAYSSAKSDRDTAESKMEEAEATYNEKQNAVPVSANEVSAAKSAYESAVSAYESAE
                     SSVTSAYNAQVSAQQTYDSAVEGQASTTRSAANSLSSADEDYQKSSLTEGDSTEELRR
                     KLEDYQESLSDYVVTAPISGIVTSLSVESGNGFSGGDLLTIQDCDSYKISTEIDEYDI
                     PDIELGQEVVIKTDATRDDELEGVVSFISPTATSSSSSSGVTYSVTIDVLTTDSRLKI
                     GMSAKLNIIVDEVTDVLTVPYDAVTENADGESVIYILDDGSMGAAGSNANMGITPDNT
                     EASDRETAEGAAAAISGSDVTEGTASDEAAGEP"
BASE COUNT          433 a          482 c          303 g          563 t
ORIGIN      
        1 ccggctcgcc tgccgcttca tctgaagccg ttccttccgt aacatcagaa ccggaaattg
       61 ccgctgctgc tccttctgca gtctctctat cggacgcttc cgtattgtca ggtgttattc
      121 ccatattggc attcgatcct gccgcgccca tactgccgtc atccagtata tagatcacac
      181 tctcgccgtc cgcattttcc gttactgcat catatggaac cgttaataca tctgttacct
      241 catccacaat aatattcagc ttggcagaca tccctatctt cagacgggaa tcagttgtca
      301 gaacatcgat cgtcacacta tatgtaacac ctgaagaact ggaacttgaa gtagctgtcg
      361 gtgaaataaa cgaaacaaca ccttccagtt cgtcgtcacg cgtcgcatct gtcttgataa
      421 ctacttcctg accgagttca atatccggga tatcatactc atcgatttcc gttgaaatct
      481 tataagaatc acaatcttgt atcgtcagga gatccccgcc ggaaaaaccg ttgccggatt
      541 ctacggaaag ggaagttaca ataccactga tcggtgcggt taccacataa tcagaaagac
      601 tttcctgata atcctccagc tttcttctta attcttctgt ggaatctccc tccgtcagac
      661 tgctcttctg ataatcctcg tccgcagatg ataaagaatt cgcggcactt cgcgttgtac
      721 tcgcctgacc ctccactgca gaatcataag tctgctgcgc acttacctgt gcattatagg
      781 cagacgttac agagctctca gcagattcat acgccgatac cgcactttca taagcagatt
      841 ttgcagcaga aacctcattt gcagacaccg gaaccgcatt ctgcttttca ttataggttg
      901 cttccgcttc ttccatttta ctctcagccg tatccctgtc agatttggct gaactatatg
      961 catcacatgc ttcatataca tcattcaacg catcagtgac cttttcggct gcggaactta
     1021 aattatttgc ttctgttcca tacgtataca gataatcacg atcctgtgcc tcagactcga
     1081 tctcatccgt ctgttcgctg acagcaatat cctcctgcag atctgcgatc gtacgattga
     1141 tatcttcatc tgaaaatgtg acgaggatgt ccccttctac tacctcatca ccgacttctg
     1201 cattgaccga tgtgatctta atatccgaca tcgccgaagt cagcgtacgt gtatcattcg
     1261 cctccacagt accggttgtc gaaatgctgt ccgttaaatc tcttttttcg atcgttgctg
     1321 tttccatttg tgagccggct tccatagcct gctccatttc ctgatttgtc ttccatttcc
     1381 ggtatacaaa aaaggcaatt gcagcaatga tcagcaatat aatcaaaact tttaatttct
     1441 ttaggatata ccctattctt ttaaatactt tttgcgcctt gacgccctta tgaagtctga
     1501 ccttcggagt ttgttcgctc aggattattc tttctgtctc atcaccctgc tccttttcaa
     1561 tccgttcctt cgtctcggcc agatcttcca ctttcgagac cggctcattc tttttctcgt
     1621 tcacgcgtct cctccgtttc ataattcttg aatagttctc taattataat aattcctctc
     1681 accattttat tcactgctta ataatttata gaacaaatgt gaaaaccaat gtcatttaga
     1741 taagcatttt ttagataaaa atcgtatatc acattcaaca g
//
LOCUS       DAOSYE010000509         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_56959_length_1777_cov_2.522067, whole genome shotgun sequence.
ACCESSION   DAOSYE010000509 DAOSYE010000000
VERSION     DAOSYE010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56959_length_1777_cov_2.522067"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLU43_08655"
     CDS             <1..467
                     /locus_tag="PLU43_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOO28521.1"
                     /translation="NTELTILCVMGSKDSEQNQQYLKTSDKFFHHGENNDGQFYKGTL
                     RAGQVLETDSSVIILGDVNPGADVISTGNIVILGTLYGHACAGANGNSDCFIVALDMK
                     PAKIQIGDYSTKTFVRGGRFLKNKQAPRIAYVLDEEIITDIITNELLSSMDF"
     gene            483..1592
                     /locus_tag="PLU43_08660"
     CDS             483..1592
                     /locus_tag="PLU43_08660"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOO28522.1"
                     /translation="MFAKYNFKKYDVLLVLLTIAISVLGIQFIGSAKEELVARQTQGL
                     ILGVTLMLIISLIDYQFILKFYWLLYIASLALLIFVKLFGDSGGGASRWFEIGGLRFQ
                     PSEIVKILLILFYAQFIMKHRETINTIRMLFACTVLIVPPFVLIYMQPDLSTSIMILI
                     IFAVVMFVGGLSYKIVIGFIVIMVPAAVIFLNMVLQPDQKILNEYQQLRILAWLQPEK
                     YATTIAYQTMNGITAIGSGQLTGKGLNNNVIASVKNGNFISAAQTDFIFAVVGEELGF
                     VGCCAVIGLLFLIVLECILIARKAKDLAGSVIAAGMAGLIGFQTFINIGVVTAILPNT
                     GLTLPFVSYGLTSLVSLFIGIGFVLNVKLQTSRYM"
     gene            1633..>1777
                     /locus_tag="PLU43_08665"
     CDS             1633..>1777
                     /locus_tag="PLU43_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOO28523.1"
                     /translation="MNIGLIAHDSKKKLMQNFCIAYRGILSKNDLFATGTTGRLIEEV
                     TNLS"
BASE COUNT          493 a          337 c          403 g          544 t
ORIGIN      
        1 acaatacgga gctcaccata ttatgcgtta tgggcagcaa agacagtgaa caaaatcagc
       61 agtacctgaa aacctcagac aaattttttc atcacggcga aaataatgac gggcagttct
      121 ataaaggaac cctccgcgcc gggcaagtac tggaaacaga ttccagtgtc atcatactcg
      181 gagatgtcaa tccgggtgca gacgttattt caaccggtaa tattgtgatc ctcggtacgc
      241 tttacggaca tgcatgcgcc ggggcgaatg gaaacagcga ttgttttatt gttgcccttg
      301 atatgaagcc tgctaagatc cagatcggtg attattcaac aaaaacattt gtcaggggcg
      361 gccgttttct taaaaataaa caggcgccgc gaattgcata cgtacttgac gaagaaatca
      421 ttaccgacat tattacaaat gaattgctaa gcagcatgga tttttaaatc agagggataa
      481 aaatgtttgc taaatataat ttcaaaaaat atgatgtact gcttgtatta ttgaccattg
      541 ccatttcggt gcttggtatt cagttcattg gcagcgcgaa ggaagagctt gtcgcacggc
      601 agacacaagg cctgattttg ggtgttacac tgatgctgat catctctttg atcgattacc
      661 agtttatatt aaaattttac tggctgcttt atattgcttc actcgccctt cttatttttg
      721 taaagctgtt cggagactcc ggcggcggtg cctccagatg gtttgagatc ggtggattac
      781 gttttcagcc gtccgagatt gttaaaattt tattgatttt attttacgca cagtttatta
      841 tgaaacatag ggaaacaatc aacacgattc gtatgctttt cgcctgtacg gtattgattg
      901 tcccgccatt tgttttgatt tacatgcagc cggacctttc cacaagtatt atgatcctta
      961 tcatttttgc cgttgtcatg tttgtcggag gccttagtta taagatcgta atcggattta
     1021 tcgtcattat ggttccggcg gcggtcatat ttttaaatat ggtgctccag ccggatcaga
     1081 agattcttaa tgaataccag cagctgcgta ttcttgcgtg gcttcagccg gaaaaatatg
     1141 caactacgat cgcttaccag acaatgaatg gtattactgc gatcggctcc ggtcagctga
     1201 caggaaaagg gctgaataat aatgtcattg cttcggtaaa gaacggcaat tttatttcag
     1261 cggcccagac ggattttatt tttgccgttg tcggagagga acttggtttt gtcggctgct
     1321 gtgctgtaat cgggctttta ttcctgatcg tactggaatg tattttgatc gcacgtaaag
     1381 caaaagatct ggcaggatct gtgattgctg caggcatggc aggtctgatc ggttttcaga
     1441 cattcattaa tatcggcgtt gtaactgcca tattgccgaa taccggtctg acacttccgt
     1501 ttgtaagcta cggactgact tcgcttgtca gcctttttat agggattggg tttgtattaa
     1561 atgttaaact gcagacatca agatatatgt aaatctgatt agtgaacgaa cataaggagg
     1621 aggaataata atatgaatat tggactgatc gctcatgatt ccaagaaaaa attaatgcag
     1681 aatttctgta ttgcttacag ggggatttta agcaagaacg atctttttgc aacaggaaca
     1741 acaggaagac tgattgaaga agtaacaaat ctttcca
//
LOCUS       DAOSYE010000510         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_56995_length_1776_cov_3.173736, whole genome shotgun sequence.
ACCESSION   DAOSYE010000510 DAOSYE010000000
VERSION     DAOSYE010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56995_length_1776_cov_3.173736"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLU43_08670"
     CDS             complement(<1..356)
                     /locus_tag="PLU43_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28524.1"
                     /translation="MTEKKKTDKKIIIAAAVLVILAVLFGALYAGFHAKGAAGSKEIQ
                     VTVVHKDLSEKEFTIKTDAAWLGEALTDEDLIAGQEGDYGLYVTEVDKETADEANQEW
                     WCITKDKETLMTGVDAT"
     gene            complement(377..1711)
                     /locus_tag="PLU43_08675"
     CDS             complement(377..1711)
                     /locus_tag="PLU43_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO28525.1"
                     /translation="MGLIQAIKGAAGSALADSWREYFYCESMDADVLVTKGAKRSNGR
                     RSSNTKGSDNIISNGSIIAINDGQCMLIVEQGKVAEFCAQPGEFVYDNSTEPSLFYGD
                     LGENIKKSFSQIGRRFTFGGDTAKDQRVYYINTKELPGNKYGTANPVPFRVVDRNIGL
                     DVDIAIRCHGEYSYKVVDPVLFYTNVSGNVESDYERDEIDSQLKTELMTALQPAFSKI
                     SDMGIRYSALPGHTMEMADALNEILSKKWTELRGLAIVSFGISSVTASEEDEQMIKDL
                     QKSAVLKDPTMAAATLTAAQAEAMKSAAANESAGPMMAFAGMNMANMAGGMNTGDLYA
                     MGAQRQAASQAAPQQAAQTQAAGWTCACGATGNTGKFCAQCGRPRPSADGWTCTCGTL
                     NKGKFCQNCGKPKPAGAPVYRCDKCGWEPEDKTHPPKFCPECGDIFDDNDKQ"
BASE COUNT          375 a          523 c          353 g          525 t
ORIGIN      
        1 gtagcatcca caccggtcat taatgtctct ttgtccttcg taatgcacca ccattcctga
       61 tttgcttcat cagctgtctc tttgtccact tcggttacat ataacccgta gtcgccttcc
      121 tgacccgcga tcagatcttc atcggtcagt gcttcaccga gccatgccgc atccgtttta
      181 atggtaaact ccttttcgga caggtcctta tgaacgaccg tgacctggat ctctttgctg
      241 ccggccgcac ctttcgcatg gaagccggca tacagagctc caaagagtac tgccagtatt
      301 accagtactg ccgccgcaat tattattttc ttatctgttt tctttttttc agtcatatgt
      361 gatctcgcaa ttattgttac tgtttatcgt tgtcgtcaaa gatgtctccg cactccggac
      421 agaatttcgg cggatgtgtt ttatcctcag gctcccaccc gcatttgtca caccggtata
      481 caggtgcgcc tgccggtttc ggttttccgc agttctggca gaacttgccc ttattcagtg
      541 ttccgcacgt gcatgtccag ccgtcggccg acggacgcgg ccttccgcac tgtgcacaga
      601 acttgccggt attcccggtc gcgccgcatg cacatgtcca gcctgctgcc tgtgtctgtg
      661 ccgcctgctg tggggctgcc tgagaagctg cctggcgctg tgctcccatt gcatacagat
      721 cacctgtatt cataccgccc gccatattgg ccatattcat gcctgcaaac gccatcatcg
      781 gtcctgcgct ttcattcgcg gccgcactct tcattgcctc tgcctgtgcc gccgtcagtg
      841 tggctgctgc cattgtcgga tcttttaata ccgcactctt ctgcagatcc ttgatcatct
      901 gctcgtcttc ctcggatgca gtcaccgaac tgataccaaa cgatacgatg gcaagccctc
      961 ttaattcggt ccactttttg gaaaggatct cgttcagtgc atcagccatt tccattgtat
     1021 gtcccggaag ggcgctgtaa cggataccca tatctgaaat tttggaaaat gccggctgca
     1081 gcgccgtcat cagctctgtc ttcagctggc tgtctatctc atctctttcg taatcactct
     1141 ctacattccc tgaaacattc gtataaaaga ggaccggatc cactacttta taagaatatt
     1201 ccccatgaca gcggatcgca atgtccacat ccaatccgat gttcctgtca acaacacgaa
     1261 acgggaccgg attggccgta ccatacttat ttcccggcag ttccttcgta ttgatataat
     1321 aaactctctg atcctttgcg gtatctccgc cgaacgtaaa acgtctcccg atctgggaaa
     1381 aggacttttt gatattttct ccaagatcac cgtaaaacag acttggctcc gtggaattat
     1441 cataaacaaa ttctcccggc tgtgcacaga actcagccac ctttccctgt tcaacgatca
     1501 gcatgcactg cccgtcgtta attgctatga tcgaaccgtt gctgataata ttatcgcttc
     1561 cttttgtatt tgaactgcgt cttccatttg accgttttgc cccttttgtt acaagtacat
     1621 ccgcatccat tgattcacag taaaaatact cccgccagga atccgccagt gcgctcccgg
     1681 ccgcaccttt aatagcctga atcaaaccca ttttaaatcc accttcctga aatataatag
     1741 ttttccgtac attataccag cctgtttata atgcac
//
LOCUS       DAOSYE010000511         1768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_57411_length_1768_cov_3.549329, whole genome shotgun sequence.
ACCESSION   DAOSYE010000511 DAOSYE010000000
VERSION     DAOSYE010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57411_length_1768_cov_3.549329"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1768)
                     /locus_tag="PLU43_08680"
     CDS             complement(<1..>1768)
                     /locus_tag="PLU43_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO28526.1"
                     /translation="LTFGSSAIIQMGTNFIFGTISFVSNSVTIVLQLALSVDYAVILL
                     NRYKEEHAVLPAREAVIVALSKAIPEISGSSLTTIGRLIAMTFMQFKMGPDMGIVLIK
                     AIILSLLSVFLLMPGIIMLFAKLMDKTKHKNFIPDIPFVGKFAYATRYLIPPIFLVLV
                     IAGFFMSSKCPYVFGYSKLETPLQNSVQTAEMMQDENFGSTNMVALVIPSGNYENEKR
                     LLADLEDCPEVDSAMGLSNIEAMDGYMLTDRLNPRQFSELLNLDYEVAELIYAAYAVN
                     DEDYAKAVNGLQTYKVPLIDMIIFMHQEVDEGYVTLDADLQEKLDDAYEQMTFAKNQM
                     QGENYSRLIVYLDLPEESDETFAFIDEMHEIAEKYYSGTILVVGDSVSQYDLQKTFSR
                     DNNVVNAVSILAVLVVLLFTFKSAGMPVLLIFVIQGSIWINFSIPTVMNTDLFFMSYL
                     IDSSIQMGANIDYAIVIASRYTELRKEMGRKDSIIKTMNVSFPTIITSGTMLTVAGIL
                     IGKRTSDGAIYGIGQCLGRGTLISIFITMFVLPQILLVGDRIIEKTAFIMNMPIRTRT
                     ASGLLRIDGVVRGRINGVVTGTM"
BASE COUNT          501 a          452 c          355 g          460 t
ORIGIN      
        1 catcgtaccg gttacaacgc cgttgatcct gcccctgaca actccgtcta tccgcaaaag
       61 tcctgatgcg gtccttgtac ggatcggcat attcataata aacgcggttt tttcaatgat
      121 ccgatcgccg acgagcagaa tctgcggcag tacgaacatt gtaataaaga tggagatcag
      181 cgtgcctcgt ccgagacact ggccgattcc gtaaatggca ccatcggatg tcctcttgcc
      241 gatcagaatg cctgccacgg taagcatcgt accggacgtt atgatcgttg gaaacgacac
      301 attcatcgtc tttataatgg aatcctttct gcccatctct ttccgcagtt ccgtataacg
      361 gcttgcaata acgatcgcat aatcgatatt ggcacccatc tggatcgaac tgtcgatcag
      421 ataactcata aaaaacagat ctgtattcat gaccgtagga atcgaaaaat tgatccagat
      481 actgccctga ataacaaaaa tcagcagtac cggcatacca gccgatttaa acgtaaacag
      541 cagaacgacc agaactgcca gaatggaaac ggcatttaca acattgttgt ctcttgaaaa
      601 tgtcttctgc agatcatact gactgacaga atccccgacg acaagtattg taccggaata
      661 atacttttcc gcaatctcat gcatttcatc gataaaggca aaggtttcgt cactctcctc
      721 cggaagatcc agatatacga tcagccggct gtaattttct ccctgcatct gatttttcgc
      781 aaaagtcatc tgctcatacg catcgtccag cttctcctgc agatccgcat ccagtgttac
      841 atacccttca tcgacttcct gatgcatgaa tatgatcata tcgatcagag gcactttata
      901 agtctgtagt ccgttgactg cttttgcata gtcttcgtca ttgaccgcat aggccgcata
      961 gatcagttcc gccacctcat agtccagatt cagaagctcc gaaaactgcc ttggatttaa
     1021 gcggtctgtc agcatatatc cgtccattgc ctcaatattg gaaagcccca ttgcggaatc
     1081 tacttccgga cagtcctcaa gatctgccag cagacgcttt tcgttttcat aatttcccga
     1141 agggatgaca agtgcaacca tattggtcga accaaaattc tcatcctgca tcatttccgc
     1201 ggtctgcaca ctgttctgaa gcggcgtttc cagtttggaa tatccaaata catacggaca
     1261 tttgctcgac ataaaaaagc ctgcgatgac cagcactaaa aatatgggag ggatcagata
     1321 tctggttgca taggcaaatt tcccgacaaa cggaatatcc ggaataaaat ttttatgctt
     1381 cgtcttatcc atcagctttg caaacagcat aataatgccc ggcattaaca gaaatacgga
     1441 taaaagactt agaatgatcg ccttgatcag cacaatgccc atatccgggc ccattttaaa
     1501 ctgcatgaac gtcatcgcga tcagtcggcc gatcgtcgtt aaggaactgc cggaaatttc
     1561 cgggatcgcc ttgctgagtg ccacgatgac cgcttctctg gccggcagga cggcatgctc
     1621 ttctttatac ctgtttaaaa gaattaccgc ataatcaaca gacaaggcaa gctgcagtac
     1681 gatcgtcacc gaattggaga caaaggagat cgttccaaat ataaaattcg ttcccatctg
     1741 aataattgcc gaagatccga atgtcaat
//
LOCUS       DAOSYE010000512         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_57469_length_1767_cov_3.500584, whole genome shotgun sequence.
ACCESSION   DAOSYE010000512 DAOSYE010000000
VERSION     DAOSYE010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57469_length_1767_cov_3.500584"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLU43_08685"
     CDS             complement(<1..486)
                     /locus_tag="PLU43_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOO28527.1"
                     /translation="MILDRIKGSNDIKQISREDYPELAEEIREFLVKKISVTGGHLGS
                     NLGAVELTMALHLVMDLPKDKIVWDVGHQSYTHKLLTGRREGFDALRKYGGMSGFPKR
                     KESPCDSFDTGHSSTSVSAGLGLVKARDIKGEDYGVYAVIGDGALTGGMCYEALNDCG
                     NT"
     gene            complement(504..1406)
                     /locus_tag="PLU43_08690"
     CDS             complement(504..1406)
                     /locus_tag="PLU43_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO28528.1"
                     /translation="MIFESELEKKTKYANEVLQKYAVLAEDGYQKTVLGAMNYSLLAG
                     GKRLRPILMDETFRMFGGEGSLIEPFMAAIEMIHTYSLVHDDLPAMDNDEYRRGKPTT
                     HIAYGEDMAILAGDALLNLAFETAAGAVAACDAKDMARASKALLILAKKAGVYGMIGG
                     QTFDVESEKKQIFLNEEQLLFIFRLKTGALIQSAMMIGAVLGGASDEELTVISEAAEN
                     IGIAFQIQDDLLDVESTQEVLGKAIGSDEKNNKITYVTIKGSQAAHKDVAMYSEKAIR
                     GLASLHHENAFLLLLVKELVTRVK"
     gene            complement(1396..1647)
                     /gene="xseB"
                     /locus_tag="PLU43_08695"
     CDS             complement(1396..1647)
                     /gene="xseB"
                     /locus_tag="PLU43_08695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOO28529.1"
                     /translation="MEITDKDKEEKEEKEEKEEVSLEEAFEKIDVTINQLQSEEIPLE
                     ESFQLYKEGMELLKYCGEKIEKVEKQVLMMDEEGEYHDF"
BASE COUNT          424 a          499 c          293 g          551 t
ORIGIN      
        1 ggtattgccg cagtcgttaa gcgcttcata acacatgccg cccgtcagcg caccatcgcc
       61 gatcactgca taaaccccgt aatcttctcc tttgatgtct cttgccttga caagaccaag
      121 acccgccgaa acggatgtgg aactgtgtcc ggtatcaaaa gagtcacacg gactttcttt
      181 tcttttagga aaaccgctca tacctccgta tttacgcagg gcatcgaagc cctcgcgtct
      241 tccggtaagc agcttatgcg tataagactg atgtccgaca tcccacacga tcttatcctt
      301 cggcagatcc attacaagat gcagagccat cgtcagttct accgcaccta aatttgaacc
      361 cagatgcccg cccgtaacgc tgatcttctt tacaagaaat tccctgatct cttccgcaag
      421 ctccggataa tcctccctgc tgatctgttt aatatcattt gaacccttaa ttctgtcgag
      481 tatcatttca atgccccgtt tctttatttc acacgtgtaa ccagctcttt tacaagcagt
      541 agcaaaaacg cattctcgtg atgcagcgat gcaagacccc tgatcgcttt ttctgaatac
      601 attgccacgt ctttatgtgc tgcctgactc cctttaatgg ttacatatgt tattttatta
      661 ttcttttcat cggacccgat cgcttttcca agtacctcct gtgtactttc cacatcaaga
      721 aggtcatcct gtatctgaaa cgcgatccca atattctcag cagcctctga aataaccgta
      781 agttcttcgt ccgatgcacc gccaagtacg gcaccgatca tcattgcgct ttgaatcaaa
      841 gcacctgttt ttaatctgaa tataaataac aattgttctt catttaagaa aatctgtttt
      901 ttctctgatt caacatcaaa tgtctgcccg ccgatcatac catagactcc cgcttttttc
      961 gcaagtatca gcagcgcttt ggacgcccgt gccatatcct ttgcatcaca tgcggcaact
     1021 gcccctgcag cagtttcaaa cgcaagattc aataaggcat ctcctgccag aatcgccata
     1081 tcttctccat aggcaatatg tgtggtcggc tttccgcgtc tgtattcatc attatccatt
     1141 gcaggaagat catcatgcac aagcgaatac gtatggatca tttcaatagc agccatgaac
     1201 ggctcgatca gggaaccctc tcccccgaac atccggaaag tctcatccat cagaatcggc
     1261 ctaagacgct taccgcctgc caaaagactg tagttcatcg caccgagcac tgttttctga
     1321 tatccatctt ccgcaagaac cgcatatttt tgaagcactt cgtttgcata ttttgtcttc
     1381 ttctcaagct ccgattcaaa aatcatggta ctctccttct tcatccatca taagcacctg
     1441 tttctcgact ttctcaattt tttcgccgca gtatttaagc agctccatcc cttctttata
     1501 tagctgaaag gactcctcaa gaggaatctc ctcagactgc agttgattga tcgttacatc
     1561 gattttttca aaggcctctt caagagaaac ctcttccttt tcttcctttt cttccttttc
     1621 ttctttatct ttatctgtta tctccatatc cattgtatgc tccttactgc cttccccaat
     1681 cactctgtaa tttccctgcc ggttacttcc gtttttaaaa tgccgttttt catatgaagc
     1741 gtcaattcct ggtgaagctc tatcctg
//
LOCUS       DAOSYE010000513         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_57523_length_1766_cov_3.032145, whole genome shotgun sequence.
ACCESSION   DAOSYE010000513 DAOSYE010000000
VERSION     DAOSYE010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57523_length_1766_cov_3.032145"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..701
                     /locus_tag="PLU43_08700"
     CDS             309..701
                     /locus_tag="PLU43_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO28530.1"
                     /translation="MAWKLDNNRAIYLQIIEEIQMRIVAGFYQPGSKLPSVRELAADA
                     AVNPNTMQKALSELENGGLIITMRTSGRIVTENTAVIQNLRKKIASEEVKLFLKKMDQ
                     YGFNKQEITGLIQDSLKGEQHEQHHNGV"
     gene            676..1377
                     /locus_tag="PLU43_08705"
     CDS             676..1377
                     /locus_tag="PLU43_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28531.1"
                     /translation="MNNIIMECKDLTKKYGGSPALNHLNLTLESGKIIGLLGPNGSGK
                     TTLIKIASGLLTPTSGTLTINGISPGVETKKRISYLPEKTYLNSWMKVSDIISYFADF
                     YEDFSKERAYTMMQNLHIEPDRKMKSLSKGTKEKVQLILVMSRNADLYLLDEPIAGVD
                     PAARDYILNTIITNYNEHAAILISTHLISDIENILDEAVFIKTGEVIMHAAIDEIREK
                     NNKSVDSLFREVFAC"
     gene            1371..>1766
                     /locus_tag="PLU43_08710"
     CDS             1371..>1766
                     /locus_tag="PLU43_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28532.1"
                     /translation="MLGKLLKQEWKALTKVLLPANLLLIIITVIGRLVVRPLTTDAES
                     SFLQVLTVFMLTFYIILLIAVTCGAFIYIVVRFYRTMYSDEGYLTHTLPLTVHQLIIG
                     KGLAASGWLIITYCLAAVNVLFLIFGAGSG"
BASE COUNT          558 a          373 c          383 g          452 t
ORIGIN      
        1 gttgagcttg cggacggcat gaccttgcag gttatggaaa tggataaaac ccggatcgat
       61 aaagtcaaaa tgacccttcc ggaaccagaa gaagaaaatg cagaaactgc agaatcttcc
      121 gacacggaaa actctgaaga ggcataaata tatttataaa tgataagacc gtatttaaca
      181 gcactgtatt taagacggtc ttttttcatt caatttttca gtaataccaa taaaaaggta
      241 ttgacaattt tacttttacg tattattgta ttatttgact aatacagtaa tggaaatgga
      301 ggaactctat ggcatggaaa ctggataata accgggcgat ctatctgcag atcatagaag
      361 aaattcaaat gcgcattgtt gcaggctttt atcagcctgg cagcaaactt ccgtctgtac
      421 gtgaactcgc tgcggatgcg gctgtcaatc cgaatacgat gcaaaaagcc ctttccgaac
      481 tggaaaacgg cggcttgatc atcacgatgc gtacaagcgg caggatcgtt accgaaaaca
      541 ctgcagttat acaaaacctg cgaaaaaaaa ttgcttctga agaagtaaag ctttttttaa
      601 aaaaaatgga tcagtacggt tttaacaaac aggaaatcac cggcctgata caggattcct
      661 tgaagggaga acagcatgaa caacatcata atggagtgta aggacctcac caaaaaatac
      721 ggaggcagcc cggcactgaa tcatctgaat ctgacattgg aaagcggcaa gatcatcggt
      781 ctgctcggtc caaacggcag cggcaaaaca actttgatca aaatcgcaag cggtcttctg
      841 acaccgacat ccggtaccct gacgatcaat gggatttctc cgggagtcga aacaaaaaaa
      901 aggatctcct atcttccgga aaaaacctat ttgaattcct ggatgaaagt cagcgacatt
      961 atcagctatt ttgccgattt ctatgaagat ttcagcaaag aacgggctta tacgatgatg
     1021 cagaatcttc atatcgaacc ggacagaaaa atgaaatctc tttccaaagg tacaaaagaa
     1081 aaagtacaat taattcttgt catgagcagg aatgcggatc tttatctgct ggatgaaccg
     1141 atcgccggtg tagaccctgc cgcaagggat tatattttaa atacgatcat taccaattac
     1201 aatgaacatg ctgctattct gatttccacg catttgatat cggacatcga aaacattctg
     1261 gatgaagcag tgtttatcaa aaccggagaa gtcatcatgc acgctgcgat cgacgaaatc
     1321 cgcgaaaaaa acaataaatc cgtagactca ttattcaggg aggtatttgc atgttaggca
     1381 aattattaaa acaggagtgg aaggcgctta caaaagtact gcttcctgcc aatctgctgc
     1441 tgatcattat tactgttatc ggaagacttg tcgtaagacc tcttacaaca gatgcggaaa
     1501 gctcttttct gcaggtgctg acggttttca tgctgacatt ttacattatt ctgctgatcg
     1561 cggtaacatg cggggcattc atttatatcg tcgtacgctt ctacaggacc atgtacagtg
     1621 acgaaggcta tctgacgcat acgctgccgc ttaccgttca ccagctgatc atcggaaaag
     1681 gactggccgc ctccggctgg ctgatcatca catactgtct tgcagcagtc aatgttttgt
     1741 tcctgatctt cggcgcaggc agcggc
//
LOCUS       DAOSYE010000514         1763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_57657_length_1763_cov_4.106557, whole genome shotgun sequence.
ACCESSION   DAOSYE010000514 DAOSYE010000000
VERSION     DAOSYE010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57657_length_1763_cov_4.106557"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLU43_08715"
     CDS             <1..556
                     /locus_tag="PLU43_08715"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO28533.1"
                     /translation="EGTALVYPLSAMGAHVSDCPNHTVGRSVPFETRGYAALAGTFGY
                     ELDVTKISEEERGMIRRQVSLYHQYNDIIRSGDYYRIASYRENHFFDCFAVVKKDRSE
                     AVITFIQVLARPNVHSKKIRLKGLKPEAEYRVSCDKNIFCASETEEKSGILTLHGDTL
                     MNAGIHIGGLSGDFAGAVIHAVEI"
     gene            578..>1763
                     /locus_tag="PLU43_08720"
     CDS             578..>1763
                     /locus_tag="PLU43_08720"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOO28534.1"
                     /translation="MKRIEIAENGMNIVFEVADNQDMKLLHFSALPFCEKDIKAPDTN
                     DGFRLVEMNLSGFDRPGERHGNKYIYTAPGYRLKYVQHSDYRNTYGRKLEFKLKDEET
                     NVHVISHMQFYDKISVVRCFNEVINQGSQPQTLEYISSFNYTGIEKEGLLPRDQKMLV
                     KIPHNSWQREMNWKTYTLVQAGLSQVQPTAFQRSSSALEVTNTGHWSTKKYLPMGFLE
                     NTETGSSLFWQIEHNGSWHWEIADQNGHLYLNISGPNEIQSHWFEELQPGDSFTTVPA
                     AVGVSTGFDAAMAEITKYRRTIRRKNADNESLKVIFNDYMNCLWADPTTEKEFPLIDA
                     AAEAGCEYFCIDAGWYAPGIWWDAVGEWKESRERFPNGMKEVTDYIRSKGMIPGVWLE
                     IEV"
BASE COUNT          509 a          317 c          495 g          442 t
ORIGIN      
        1 ggagggcacg gcacttgtct acccgctttc cgcaatgggg gcgcacgtca gcgactgccc
       61 gaaccatacg gtcggcagaa gtgtgccgtt tgaaacaagg gggtatgctg cgcttgccgg
      121 tacattcggc tatgagctgg atgtgacaaa gatctcagag gaagagagag ggatgatacg
      181 caggcaggtg tcactgtatc accagtacaa tgatattatc aggagcggcg attattaccg
      241 gatcgcctct tatcgtgaga atcatttttt tgattgtttt gcagttgtaa aaaaggaccg
      301 gtcggaagcg gttattacgt ttattcaggt gcttgcaaga cccaatgtcc atagcaagaa
      361 gatccgtctg aaaggactga aaccggaggc agaatatcgt gtatcatgtg ataaaaatat
      421 tttttgtgca tcagaaacag aggaaaagag cggaatactt acgctgcatg gcgatacact
      481 gatgaatgca ggcatccata tcggaggcct ttcgggtgat tttgcagggg cagtcattca
      541 tgcggtggaa atatgatgat aaggagcagg ggtaatcatg aagagaattg aaattgcaga
      601 aaatgggatg aatattgtat ttgaagtagc ggataaccag gatatgaagc tgctgcattt
      661 ttcggcgctg ccgttttgcg aaaaagatat taaggcgccg gatacgaacg acggatttcg
      721 tcttgtggaa atgaatttgt cggggtttga ccgtccaggt gaacggcatg ggaataaata
      781 tatttatacg gctcccggtt atcgtctgaa atatgtacaa cacagcgatt ataggaatac
      841 atatggacgg aagctggagt ttaaactgaa agatgaagaa acaaatgtcc atgtgatcag
      901 ccatatgcag ttttatgata aaatatcggt cgtacgctgt tttaatgagg tgattaacca
      961 ggggagtcaa ccacagacgc tggaatatat ctcttccttt aattatacgg gaatagaaaa
     1021 agaagggctg cttccaagag atcaaaaaat gcttgttaaa attcctcata attcatggca
     1081 gcgggaaatg aactggaaga cttatacgct tgtacaggcg ggtctgtcac aagtacagcc
     1141 gactgcgttc cagcgttctt cttcggcttt ggaggtaacg aataccgggc attggtccac
     1201 taagaaatat ctgccgatgg gatttctgga aaatacggag acaggcagca gtctgttctg
     1261 gcagattgag cataacggtt cctggcactg ggaaatagct gaccaaaacg gtcatttata
     1321 tttgaatatc agcggtccga atgagatcca gtcgcactgg ttcgaagagc tgcagccggg
     1381 agattccttc actacggtgc ctgctgcagt aggcgtgagt acgggatttg acgcagcaat
     1441 ggcagagatt acaaagtatc ggcggacgat ccgaagaaaa aatgcggata atgaatcttt
     1501 aaaggtgatt tttaatgatt atatgaattg tttatgggcg gatccgacaa cggaaaagga
     1561 gtttccgctg atcgatgcgg cagcggaagc tggctgtgaa tatttctgta tcgatgcagg
     1621 atggtatgca ccggggatct ggtgggatgc ggtcggtgaa tggaaagaat ccagagagcg
     1681 ttttccaaac ggtatgaaag aggttacgga ctatatccgc agtaaaggga tgattcctgg
     1741 cgtctggctg gaaatcgaag tga
//
LOCUS       DAOSYE010000515         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58065_length_1756_cov_2.291593, whole genome shotgun sequence.
ACCESSION   DAOSYE010000515 DAOSYE010000000
VERSION     DAOSYE010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58065_length_1756_cov_2.291593"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..805
                     /locus_tag="PLU43_08725"
     CDS             44..805
                     /locus_tag="PLU43_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOO28535.1"
                     /translation="MDIFQDYDIVYVVTDTKPFIEDKTWIDRFGERLFMQYPDESPWS
                     EDKENCYGWLMQFADGNRIDLHVESIVYAKEYIQTDKLCKILLDKDGILPEIPEATDE
                     DYWVKKPSLEQYLGVCNEFWWCLDNVAKGLWREEIPYVQDMLNFYVRKQLEKMLTWKI
                     GILTDFSVSVGKSGKYMFKWLSAQEWNAYLETYPAGTADAIWIATEQMCRLFEDTAIW
                     TGRQLGYVYDLKEGRNCQEFLQHVKALPKDAREIY"
     gene            847..1755
                     /locus_tag="PLU43_08730"
     CDS             847..1755
                     /locus_tag="PLU43_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO28536.1"
                     /translation="MYLDTFKLPVGKEEQIIKKRMVHNGGPYGYIDNIYPCGIFSQKQ
                     LCRIDFERITILYGGNGSGKSTLLDLIANKLELHRTAPFNSSELFSQYTYECEYTLGY
                     DEEGSRYKVPNGSRIIKSDDVFDYMLAARENNQDIAENIEDAREEWGSLKFGKTVKFS
                     GMEDYEANRLQVLARSKSLTRRQFISRTAGAEVKLNSNGETALEYFDSKLKNDTLYCL
                     DEPENSLSPKLQMKLKDMIEKMSRYCGCQFILATHSPFLLSLSQAKIYDLDETPVDIK
                     KWWELENPKIYFDFFDQYRKLFDTDA"
BASE COUNT          577 a          269 c          442 g          468 t
ORIGIN      
        1 gaaaaccact aaaattaaat tgaattaagc ctaactaaat ggcattgata tttttcagga
       61 ttatgatatt gtatatgtgg tcacagatac aaagccattc attgaggaca agacgtggat
      121 cgaccgtttc ggagaaagac tgtttatgca gtatccggat gaatcgccgt ggtcggagga
      181 taaagaaaat tgttacggct ggctgatgca gtttgcggat ggaaatcgta ttgacctgca
      241 tgtggaaagt attgtgtatg caaaagagta tatacaaacc gataagcttt gcaaaattct
      301 gctggataag gatggcatac ttccggaaat tccggaagca acagacgagg attattgggt
      361 aaaaaagcct tctttagagc agtacttagg tgtctgtaat gaattctggt ggtgtctgga
      421 taatgtggca aaaggacttt ggcgggaaga aataccatat gtgcaggaca tgctgaattt
      481 ttatgttaga aaacagcttg aaaagatgtt aacatggaaa atcggtattc tgactgattt
      541 ttctgtcagt gtcggaaaat ccggaaaata tatgtttaaa tggctgtctg cgcaggagtg
      601 gaatgcgtat ttggaaacgt atccggcagg aacagcggat gcgatctgga ttgcaacgga
      661 gcagatgtgc agactgttcg aagatactgc aatatggaca ggcaggcaac tgggatatgt
      721 atatgatctg aaagagggaa gaaactgtca ggaatttctg cagcatgtga aagccctgcc
      781 aaaagacgcc agagagattt attgatttta gggaagggca gattatgacc gttatggagg
      841 aaacgcatgt atttggatac ttttaaatta cctgttggaa aggaagagca gatcataaaa
      901 aaacggatgg ttcataatgg cggtccatac ggctatatag ataatatcta cccgtgcggt
      961 attttttccc aaaagcagtt atgcaggatc gattttgaaa gaattacgat cttatacggc
     1021 ggaaatggat ccggtaaaag tacgctgctc gacctgatag ccaataaact ggagcttcac
     1081 aggacggcac cgtttaattc atcggagctt ttcagtcaat acacgtatga gtgcgaatat
     1141 acgctcgggt atgatgaaga agggagcagg tataaggtcc caaatggaag cagaatcata
     1201 aagagtgatg atgtctttga ttatatgctt gctgcaagag agaacaatca ggatattgct
     1261 gaaaacattg aagatgcaag agaagaatgg ggaagcttaa agtttggaaa aaccgtaaaa
     1321 ttcagtggaa tggaagacta tgaggcaaat cgattacagg tactggcacg ttcaaaatct
     1381 ttgacaagaa gacagtttat cagcagaact gcaggcgcag aagtcaagct gaacagtaat
     1441 ggagaaacgg cgcttgaata ttttgattca aaattgaaaa atgacacact gtattgtctg
     1501 gatgaaccgg aaaatagtct gtcgcccaag ttacagatga aactgaaaga catgattgaa
     1561 aaaatgtcac ggtattgcgg gtgtcagttt attttggcga cacattcccc atttctgctg
     1621 tcattgtcac aggctaagat ctatgatctg gatgaaacgc cggttgatat taaaaaatgg
     1681 tgggaacttg agaatcctaa aatttatttt gatttttttg atcaatatag aaagctgttc
     1741 gatacagatg cctgat
//
LOCUS       DAOSYE010000516         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58101_length_1755_cov_2.920000, whole genome shotgun sequence.
ACCESSION   DAOSYE010000516 DAOSYE010000000
VERSION     DAOSYE010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58101_length_1755_cov_2.920000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PLU43_08735"
     CDS             complement(<1..1404)
                     /locus_tag="PLU43_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28537.1"
                     /translation="MVANHVYLSPQYFSKLFKKVTGIGFLNYLTNIRLNHSLLKLSQS
                     DDTILKVAVSNGFANSKAYSIAFKKNFGVLPSAYRKSHGRIDGEKGNQELVTKFDLDL
                     MDAGLDFLGLFKNEDAMLTSPEQVVYINTKNREKSCDGIHVQQKVLLLRHIQTILKAE
                     SLEQLKYVQKHLGFSYIYLQIEEEIMDIWFNSDRIFALHQCCNIIEILCDLNLVPIFC
                     LDVDLIMRNYNKEGGAELLLDNILKIFGHLERVTAMKKFLHQCRFEISWKKQGDYKFF
                     SQFVSLFIKKFQPIVGEERIGLLALRDDLPESLHHFEEQLKSFNSTGWFPGFFSFYAV
                     PNKELPNEGYKELYSRMVSDLKGCIRTVAPEKLQKKNEIEIFMMKWDTLMGKSRLELM
                     SFFRPALILDSALSIYSKLYGIGFYLDTLEKMEKEFHSDSIDLALFLKRKIKRPLYFV
                     VEVLNRMGKQIIYQDNQL"
     gene            complement(1420..>1755)
                     /locus_tag="PLU43_08740"
     CDS             complement(1420..>1755)
                     /locus_tag="PLU43_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28538.1"
                     /translation="HQNVVLKLELCVNFLEREGADFLEYGYSCYSGEESEDKKTAYYE
                     LRQNMMQQLITYVDKKQGYRLEQKKILYSIISCLYKYFRQEKFILPQRCNETRIVFEA
                     QHYINKNYD"
BASE COUNT          527 a          391 c          273 g          564 t
ORIGIN      
        1 aagctgattg tcctgataga tgatctgctt tcccattctg tttaatactt ctactacaaa
       61 ataaagcggt cttttgatct ttcgcttaag aaaaagtgca agatcgattg agtctgaatg
      121 gaattccttt tccatctttt ccagcgtatc cagataaaaa ccgatcccat aaagctttga
      181 ataaatactg agcgcgctgt caagaatcag cgcaggacgg aaaaagctca tcaattccaa
      241 acggctcttt cccataagcg tatcccattt catcataaat atttcaattt catttttttt
      301 ctgtaatttt tccggagcaa cagtccggat acagcctttc agatcgctta ccatacggct
      361 gtataattcc ttataccctt cattcggcaa ttctttattc ggaaccgcat aaaagctgaa
      421 gaaaccagga aaccagcctg tactgttaaa acttttcaac tgctcctcaa aatgatgcag
      481 gctctccgga aggtcatccc taagtgccag aagaccgatc cgttcttctc cgacaatagg
      541 ctgaaacttt ttaataaaaa gagacacgaa ctgggaaaag aatttataat caccttgttt
      601 cttccatgaa atttcgaacc ggcactgatg cagaaatttc ttcatcgccg ttacgcgttc
      661 aaggtggccg aatattttta aaatattgtc cagaagcagc tctgctcctc cttctttatt
      721 ataatttcgc ataatcaagt caacatccag acaaaaaata ggcaccagat tcaaatcaca
      781 cagtatttct ataatattgc agcattgatg cagtgcaaat atcctgtcgg aattaaacca
      841 gatatccatg atctcttctt ctatctgaag ataaatataa gaaaagccaa gatgcttttg
      901 tacatatttt aactgctcca gactttctgc ctttaatatc gtctgaatat ggcgcagcaa
      961 aagaaccttt tgctgcacat gtatcccgtc acatgatttt tccctgtttt ttgtattgat
     1021 ataaacaacc tgctccggac tcgttaacat tgcatcctca tttttaaaaa gccccagaaa
     1081 gtccaatccg gcatccatca aatccaggtc gaatttagtg acaagttctt gatttccctt
     1141 ctctccatca attctcccgt gtgattttct atatgcactc ggcagcactc caaaattttt
     1201 cttaaacgca atagaatagg cttttgaatt tgcaaatcca ttggaaacag ctacttttaa
     1261 gatcgtatcg tctgactgcg acagctttag cagggaatga ttcagacgaa tatttgtcaa
     1321 atagttcaag aaaccgattc cggttacttt tttaaataat tttgaaaagt actgcgggga
     1381 taaatagaca tgattggcaa ccatccctaa tgtaattttt caatcataat ttttgtttat
     1441 ataatgctgc gcttcaaaaa caattcttgt ttcattgcac cgctggggca gtatgaattt
     1501 ttcctgacga aaatatttgt aaaggcagct gataatagaa tataatatct ttttttgttc
     1561 cagtctgtac ccctgctttt tatcaacata cgtaatcagc tgctgcatca tattttgtct
     1621 aagctcataa taggctgttt ttttatcttc actttcctca ccggaataac agctataccc
     1681 atattccaga aaatcagcgc cctccctctc cagaaaattc acacatagtt ccagttttaa
     1741 tacaacattc tgatg
//
LOCUS       DAOSYE010000517         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58204_length_1753_cov_3.579505, whole genome shotgun sequence.
ACCESSION   DAOSYE010000517 DAOSYE010000000
VERSION     DAOSYE010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58204_length_1753_cov_3.579505"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1753
                     /locus_tag="PLU43_08745"
     CDS             <1..>1753
                     /locus_tag="PLU43_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO28539.1"
                     /translation="LSPSTRNKPALFLYFFIGYDVIIKAGKNIRHGQIFDENFLMMAA
                     TFGAFGVGENAEAIAVMLFYQIGELFQSYAVGKSRRSIAAMMDICPEYANIEENGRLR
                     RVDPDEVEPGSIIVVKPGEKIPLDGIVTEGESFIDTTALTGEAVPRKAAVKDMVISGC
                     INGSGTLQVRVTKNFENSTVAKILELVENAGSKKTKTENFISKFAKYYTPVVTIGAIL
                     LAFLPPLILGGGWGDWIQRACIFLVISCPCALVLSVPMGFFGGVGAASGQGILIKGSN
                     YLETVEKMTTIVFDKTGTLTKGEFKVQNLLPQNCSKEELLEMAALGEGFSNHPIAVSI
                     KEAYGKEPDMSRVFGAEEIAGKGICVTVDGRELLLGNGKLMEERNIVYVPVKGSGGTV
                     VYVAEEGIYAGAVVISDAIKEGAGSVLRQMKQLGVKKCVMLTGDGKEAAGRVAKELGI
                     DEVYADLLPQDKVTIVERLLKEQKEQERLAFVGDGINDAPVLTRADIGIAMGSMGSDA
                     AIEAADIVLMDDSIEKILSVMKIAEKTLRIVRENIVFALSVKLLVLLLGTFGLANMWE
                     AVFADVGVSVIAILNAMR"
BASE COUNT          477 a          324 c          517 g          435 t
ORIGIN      
        1 ttttatcgcc ttcaaccagg aataaacctg cactgtttct gtattttttt atcggctatg
       61 acgtcataat aaaggcaggt aaaaatattc gtcacggaca gatctttgat gaaaattttt
      121 tgatgatggc tgcgaccttc ggcgcattcg gagtcgggga aaatgcagaa gcgattgcag
      181 ttatgctgtt ctatcagatc ggggagttat tccaaagcta tgcggtcggt aaatcccgcc
      241 ggtcaattgc agcgatgatg gatatttgtc cggaatatgc caatattgag gaaaacggaa
      301 gattgcgccg ggtggatccg gacgaagtgg aacccggcag tattattgtg gtcaaaccgg
      361 gcgagaagat ccccttagac ggaatcgtga cagaggggga atcctttatt gatacgacag
      421 cgctgaccgg agaggctgtt ccgagaaagg cggcagtaaa ggacatggtc atcagcggat
      481 gcatcaacgg cagcggtacg ctgcaggtaa gggtcacgaa gaatttcgag aattcgacag
      541 tggcaaaaat attggagctt gtagaaaatg caggcagtaa aaaaacaaaa acggaaaatt
      601 ttatttcaaa atttgcaaag tattatacac ctgttgtaac gatcggtgcg attcttcttg
      661 catttcttcc accgctcata cttggaggcg gatggggaga ctggatacag cgtgcatgca
      721 tctttctcgt tatttcctgt ccctgtgcac ttgtgctttc tgttccgatg ggatttttcg
      781 gaggggtcgg tgctgcgtcc ggacagggaa tactgatcaa ggggagcaat tatctggaaa
      841 cagttgaaaa gatgacaacg atcgtatttg ataagaccgg tacgctgaca aagggagaat
      901 tcaaagttca gaacctgctg ccgcaaaatt gttcaaagga agaattactg gagatggcgg
      961 cgcttggaga agggttttcc aatcatccga tcgccgtatc cattaaagag gcttatggaa
     1021 aagaaccgga tatgagccga gttttcggag cagaggagat cgccggaaaa ggaatttgtg
     1081 taacagttga cggcagggag ctgctgcttg gaaacgggaa actgatggag gaaagaaata
     1141 ttgtgtatgt gcctgtcaaa ggatccggcg gtacggttgt ttatgtggca gaagaaggga
     1201 tctatgccgg tgcggtcgtt atttccgatg cgataaagga gggagcaggg agtgtactta
     1261 ggcagatgaa gcagcttggt gtgaaaaaat gtgtcatgct gacaggcgac ggaaaggaag
     1321 cggccggccg cgttgcgaag gaacttggca ttgatgaagt atatgcggac ctgctgccgc
     1381 aggacaaagt gacgatagtc gagcggcttc ttaaagaaca aaaggaacag gaacggcttg
     1441 catttgtcgg agacggcatt aacgatgcac cggtgctgac ccgtgcggat atcggtattg
     1501 caatgggcag tatgggttcc gatgccgcaa tcgaagccgc ggatatcgta ctgatggatg
     1561 acagtattga gaaaattctt tccgttatga aaatagcaga aaagacgctg cgcattgtca
     1621 gggagaatat tgtctttgct ctgtcggtga agcttcttgt actgctgctt ggcacatttg
     1681 ggcttgcaaa tatgtgggaa gccgtgtttg cggatgtcgg tgtttccgtg attgcgatac
     1741 tgaatgcaat gcg
//
LOCUS       DAOSYE010000518         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58248_length_1752_cov_4.117855, whole genome shotgun sequence.
ACCESSION   DAOSYE010000518 DAOSYE010000000
VERSION     DAOSYE010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58248_length_1752_cov_4.117855"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLU43_08750"
     CDS             complement(<1..1099)
                     /locus_tag="PLU43_08750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOO28540.1"
                     /translation="MLIYDPKNRINGQIMKTGIEKIWNHKTLRRMILVLTAVMALISV
                     VQGIRNAAAFSQDFQWDAVKAFTLKINPYEESLHPSGALSRYGFEKYFKQMEANQFPS
                     LLMLLIPYTFLPPLAARYAWIISNLLFTAGIIWLLRKTFMKAVEKDVFVLLILLMLAG
                     TPYRNQLGVGQHTLFAFFFFLLAVYFSEKENRQIPMILSLTVCYFKYTLTVPLALYFV
                     YKRRWKELTVSVLIHVGLTEIAAHWLGTGFMDMIIEPLKVSSALVSEGGLDFGALFSG
                     SSFSFILAGMVMLLLFVLVLFMPQKEDCLVIGILTLWALIITYHRTYDFFVLIIVAAL
                     FYEKKRSDFLKSAYAVVLLAVFFVLRLFSESA"
     gene            complement(1086..>1752)
                     /locus_tag="PLU43_08755"
     CDS             complement(1086..>1752)
                     /locus_tag="PLU43_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOO28541.1"
                     /translation="LVKTIVTTNLADELTEAYHVKLIEVLTGFKYIGEQIKLFEQNHT
                     YQYVFGLEESYGCLAGTHARDKDAVVAVMCLCEVAAFCKYHGITVWDQMLKIYEEYGY
                     YMEGMHTITLKGIEGAGKIQEIMNKLRSNPPKAFGSLKVLKFRDYSEDTILDMETGSR
                     TPTGLPSSNVLYFELENNSWCCARPSGTEPKIKFYIGIKGTSLADAKERLDELKQAVV
                     ADI"
BASE COUNT          529 a          443 c          338 g          442 t
ORIGIN      
        1 cggcggattc gctgaacagc cgaagaacaa aaaatactgc cagcaataca accgcataag
       61 ccgattttaa aaaatccgac cgcttctttt cataaaataa agccgctaca ataatcaaga
      121 caaaaaaatc ataagtcctg tgataggtga tgatcaaggc ccacaatgtc agtatcccga
      181 tcacaagaca atcctctttt tgaggcataa aaagcactag cacaaacaac agcagcataa
      241 ccataccggc cagtataaaa gaaaaagaag aaccggaaaa cagtgcgccg aaatcaagac
      301 cgccttcgga aactaatgca gaagagactt ttaacggttc aatgatcatg tccatgaacc
      361 ctgtcccaag ccagtgagct gctatctcgg tcagccctac atgaataaga acagagaccg
      421 tcaattcttt ccatcttctt ttatatacaa aatatagagc gagcggaacc gtcagtgtat
      481 acttaaaata gcagacagtc aggctgagta tcatcggaat ctgccggttt tccttttcag
      541 aaaaataaac ggcaagcaaa aagaagaaaa aagcaaacag tgtatgctgt ccgaccccca
      601 gctgatttct gtacggcgtt cccgcaagca tcagcagaat cagaagtaca aacacatctt
      661 tttcgactgc cttcataaaa gttttgcgga gcagccatat gattcccgca gtaaacaaaa
      721 gattcgatat gatccatgca tatctcgccg caagcggcgg taaaaatgta taggggatca
      781 aaagcattaa aagagaggga aactgatttg cctccatctg cttgaaatat ttctcaaagc
      841 cgtaccggct caaagccccc gaaggatgca gtgattcctc gtaaggattg atcttcagcg
      901 tgaatgcctt tacggcatcc cactgaaaat cctgactgaa cgcagccgca ttccggatgc
      961 cctgcactac tgaaataagt gccatgaccg cagtcaatac aaggatcatc ctgcgtaatg
     1021 ttttatgatt ccaaatcttt tcaatacctg ttttcataat ctgaccattt atccgattct
     1081 ttggatcata tatcagcaac gaccgcctgt tttaactcat caagccgttc ctttgcatct
     1141 gcaagactgg ttcccttaat tccaatataa aacttaattt taggctcggt tccggaaggt
     1201 cttgcgcagc accaggagtt attttccaat tcgaagtaca gtacattgga gcttggaagg
     1261 cctgtcggcg tcctgctgcc ggtctccata tccaaaatcg tatcctcgct gtaatccctg
     1321 aatttaagca ccttcagact gccaaaggct ttcggcggat tgctgcgaag cttgttcatg
     1381 atctcctgta ttttaccggc accttcaatc ccctttaacg tgatcgtatg catgccttcc
     1441 atgtagtaac catattcttc gtagatcttc agcatctgat cccatacggt gatcccatga
     1501 tatttgcaga aagcggcaac ttcacaaaga cacatcacgg caacgaccgc atccttgtcc
     1561 cgtgcatgag taccggccag acagccgtag ctttcttcaa gaccaaacac atactgatat
     1621 gtatgatttt gctcaaacag tttgatctgt tccccgatat acttgaatcc ggtaagcacc
     1681 tcgatcagct tcacatgata agcctctgtc agctcatcgg ccagattcgt cgtaacgatc
     1741 gtcttgacga gc
//
LOCUS       DAOSYE010000519         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58596_length_1746_cov_3.156712, whole genome shotgun sequence.
ACCESSION   DAOSYE010000519 DAOSYE010000000
VERSION     DAOSYE010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58596_length_1746_cov_3.156712"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLU43_08760"
     CDS             <1..1282
                     /locus_tag="PLU43_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28542.1"
                     /translation="FVLQTASAATAIALRQYIYARLKTEEFISLDLASFTEEELQHRA
                     DSLCEAWEITIETTGIAGDLADQSLYMLDASTAGQEEPNVKAWAENLMKQCDAAQGGQ
                     TLRQLARQLNTDAGPAHEQLVLMQESIYNETMSAESLDKMTQIAQAAETACKVGLFTS
                     ADAEGDVMLSGVTCIADVADTGSSIILGENNRVTVSYKDIREKLAPVSTVVGLITLGS
                     SDMGEQLFYIGDTLADWYCDGKVIGIGISGTEDGKNHMKAALVDAQTASDGEIEALLE
                     EEGIPMSEQSGLAPVQLLQEYGRSTEEIFADLDSLTTQMAYFEMNDEQESESSMADMT
                     NVDISGSYSGVLIGEDGEESQSEPVWIEDNHDGTITMLDEEGGETVMNYDSRTGEIIE
                     DGFYLKFSESDGGITADGYNEEYDFSISLYRTGF"
     gene            1405..>1746
                     /locus_tag="PLU43_08765"
     CDS             1405..>1746
                     /locus_tag="PLU43_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein"
                     /protein_id="HOO28543.1"
                     /translation="MKHLLDFIEVRLDDFKIKKKLYILYILCVLFPLILTDSVIIYTV
                     INSEQTDRKHGMENIANAVQYFLDSRVDSAAKLGKSIFINRYIDEFLNKEYADSLDYV
                     TDYQNFFKDTLF"
BASE COUNT          536 a          287 c          481 g          442 t
ORIGIN      
        1 ttttgtattg cagacagcaa gtgcggcgac agcgattgcg ttacggcagt atatttatgc
       61 ccgcttaaag actgaggaat ttatatccct ggatctggct tcctttacgg aagaggaatt
      121 gcagcacagg gcagattccc tttgcgaagc atgggaaatt acgattgaga ctacgggtat
      181 tgcaggggat ctggctgatc agtccttata tatgctggat gcatcgacag ccgggcagga
      241 ggagcccaat gtaaaagcat gggctgaaaa tttaatgaag cagtgtgatg cggcccaagg
      301 aggacagacg ctccgtcagc tggcgaggca gcttaatacg gatgcagggc ctgcccatga
      361 gcagcttgta ctgatgcagg agagtattta caatgagaca atgagtgcgg aatccctgga
      421 taaaatgacc caaatagcgc aggcagcaga aacagcatgt aaagtagggc tgtttacgag
      481 cgcagatgct gagggtgatg ttatgctcag cggcgttacc tgtattgcag atgtggctga
      541 taccgggagc agtattatat tgggagagaa caacagagta acggtatcat ataaagacat
      601 acgggagaag cttgcaccgg tttccacagt agtcggtttg atcacgcttg gttcttccga
      661 tatgggggaa cagctgtttt atatcggaga tacactggcc gactggtatt gtgacggtaa
      721 agtcataggg atcggaatat ccggaaccga ggatggaaaa aatcatatga aagcagccct
      781 tgtagatgcg cagacggcat ctgacggtga aatcgaagcg cttttagaag aagaaggcat
      841 accaatgtca gaacagtcag gtctggcgcc tgtacagctg ctgcaggaat atggacgttc
      901 cacggaagag atttttgcag atctggattc cctgacaaca cagatggcat attttgaaat
      961 gaacgatgag caggaaagcg aatcttctat ggcagatatg acaaatgtag atatatcggg
     1021 cagttacagt ggggttctga tcggggaaga cggagaggaa agccagtccg agcctgtttg
     1081 gatagaagac aatcatgatg ggacgatcac gatgctggat gaagaagggg gagaaactgt
     1141 catgaattat gacagccgta caggagagat cattgaggat gggttttatt taaagttcag
     1201 cgaatcggat ggaggaatca cagcggatgg atataatgaa gaatatgatt tttccatatc
     1261 actttaccgg acaggttttt gagtatgatg acggaagatg atgttttgac agatgccaat
     1321 aaagcaagaa atgggagtga gaaagcaata attgaacaga aatcagggct gtaatttttt
     1381 atagtaaaat atagaggaaa gcagatgaag catttattag attttattga agtcagactg
     1441 gatgacttta aaatcaagaa aaaattatat attttatata ttctgtgtgt acttttcccg
     1501 ctgattctga cggatagtgt gatcatttat actgtgatca attcggaaca gacagataga
     1561 aagcatggga tggagaatat tgcaaatgcg gtgcagtatt ttctggacag ccgtgtagac
     1621 agtgcggcaa aattgggaaa gagcattttt atcaacagat atattgatga gtttctgaat
     1681 aaagaatatg ctgattccct ggattatgta acggactatc agaatttttt caaggacacc
     1741 ctgttt
//
LOCUS       DAOSYE010000520         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58604_length_1746_cov_2.964518, whole genome shotgun sequence.
ACCESSION   DAOSYE010000520 DAOSYE010000000
VERSION     DAOSYE010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58604_length_1746_cov_2.964518"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1170
                     /locus_tag="PLU43_08770"
     CDS             157..1170
                     /locus_tag="PLU43_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO28544.1"
                     /translation="MSETIDLKKIPQRILYTGAKMPAVGLGTFGSDKYSPEQISEAVY
                     GAVKAGYRLVDCASVYQNEDQIGAVLRSLFDDGVVTREELFITSKVWNDMHGDGEVIA
                     SCKKSLADLRLDYIDLYFVHWPFPNYHAPGCDGDSRNPNSRPFSVGEFMETWKQCEQL
                     VDMGLVKHIGMSNMTIPKLDAVLPLCTIRPAAIEMELHPSFQQPELFAYVQAHGIVPI
                     GYCPIGSPSRPERDRTAEDVADTEMPEVVRIAQAHQVHPAIVCLKWAVQNGQIPIPFS
                     VKEPQYISNLKCCTEDPLTDAEMETLEKADKNCRLVKGQVFLWPGAKDWTDLWDLDGT
                     ITK"
     gene            1318..>1746
                     /locus_tag="PLU43_08775"
     CDS             1318..>1746
                     /locus_tag="PLU43_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO28545.1"
                     /translation="MKQKKTRVICFANNKGGSGKSTSCSNVGAGLAGRGKKVLLLDGD
                     MQMNLTLSFFTEEKVLAFAKSERNLYAAVKAQNDLTGYTIPTDYENLDLIPSSTLMSS
                     IEYELFSKRQHELLLKRMLQGVRESGDYDYILIDAPPTLGI"
BASE COUNT          486 a          348 c          497 g          415 t
ORIGIN      
        1 cttgtacgct ggggcattca tccggaagac ctgatcacgg atgagttccc gcttgaaaaa
       61 gcagatgaag catatgcgct gatggccgga ggacactgcg gaaaggttgc ggtcgtattc
      121 ggcgatcagg atgagaaata acagggggtg cccaaaatga gtgaaacgat tgatttgaaa
      181 aaaattccgc agagaatact ttatacggga gcaaagatgc cggcggtagg acttgggaca
      241 ttcggttccg ataaatattc accagagcag atatccgaag ccgtatatgg tgcggtcaaa
      301 gcgggttacc gtcttgtaga ctgtgcgtcc gtttatcaga acgaagatca gatcggtgct
      361 gttttacgga gtctttttga tgacggcgtt gtaacaagag aggaattgtt cattacctca
      421 aaggtgtgga atgatatgca cggcgacggg gaggtcattg cttcctgcaa gaaaagtctt
      481 gcggatctga ggctggatta tattgatctg tattttgtac actggccgtt tccgaattat
      541 catgcgcccg gctgcgacgg ggattccaga aatccgaatt ccagaccatt cagcgtcggg
      601 gagtttatgg agacatggaa acagtgcgag cagcttgtgg atatgggact cgttaagcat
      661 atcgggatgt ccaatatgac gattccaaaa ctggatgcgg tactgccgct ttgtaccata
      721 cggccggctg cgatcgaaat ggaactgcat ccaagctttc agcagccgga attatttgca
      781 tatgtacagg cacatggtat cgtaccgatc gggtactgtc cgatcggttc tccttccaga
      841 ccggaaagag accggacggc agaggatgtt gcggatacgg aaatgccgga ggtggtaagg
      901 atagcgcagg cgcatcaggt gcatccggca atcgtctgcc tgaaatgggc ggttcagaac
      961 ggtcagatac cgataccgtt ttcagtaaag gaaccgcagt atatctccaa tctgaagtgc
     1021 tgtacggaag atccgctgac ggatgctgag atggaaacac tggaaaaggc tgataaaaac
     1081 tgccgtcttg taaaaggaca ggtattttta tggccgggag caaaagactg gaccgacctt
     1141 tgggatctgg acggtacgat tacgaaataa agattgcata agcagagatg accgtcagga
     1201 tacgaatgtg ttttggcggt ctctgctttt cgttctaaaa tatgtacgta ctgcagggaa
     1261 tatgatacaa tgttattata ctgaaaacgt cggcagatgc aggcagaagg agagcaaatg
     1321 aagcaaaaaa agacaagggt catctgtttt gcaaacaaca aaggcggaag cggaaaatca
     1381 acgtcctgtt cgaatgtggg agccggactc gccgggcgag ggaaaaaagt gcttttgctc
     1441 gacggtgata tgcagatgaa tctgacactc tcttttttta cggaagaaaa ggtgcttgca
     1501 tttgcaaaaa gtgagcggaa tctgtatgcg gcagtaaagg cacagaacga tctgaccggt
     1561 tacacgattc cgaccgatta tgaaaatctg gatctgatcc cgtcttcaac gctgatgagt
     1621 tccattgagt atgaactgtt ttcaaaacgg cagcatgagc tgctgctgaa aaggatgctg
     1681 caaggtgtca gggagtccgg agactatgat tatatcttga tcgatgcacc gcctacgctt
     1741 gggatc
//
LOCUS       DAOSYE010000521         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58609_length_1746_cov_2.740982, whole genome shotgun sequence.
ACCESSION   DAOSYE010000521 DAOSYE010000000
VERSION     DAOSYE010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58609_length_1746_cov_2.740982"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLU43_08780"
     CDS             complement(<1..400)
                     /locus_tag="PLU43_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HOO28546.1"
                     /translation="MEWLMKSGEMRRRKHAVGRGFAWFGISMWYLLLYVPIVVMIIYS
                     FNTQKQNIIWQGFTTSWYGKLFLDKELMTILSNTLIVSIFSTIISVIIGTMAAYGITR
                     YKFKGKQLLMNIMYVPIVIPEIILGVSLLMV"
     gene            complement(458..1369)
                     /locus_tag="PLU43_08785"
     CDS             complement(458..1369)
                     /locus_tag="PLU43_08785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28547.1"
                     /translation="MTETKLSQRNTERQKFLKKKQFKTNTIPAIITVGPIALWLLAFI
                     VIPMIYVIVISFMSQGDYGYIQYQLTTENYTEMLQPTYLRIFGITIVIAIISTITVLL
                     ISYPIAYFIAKQKGSRGGYMIMLMMIPSWTIGLVRTYSWVRILQNKGILNGLLQWLKL
                     IDSPVQFLYNNGAVAFGTILSLMTFSILPLYSSIEKLDDSFLEASKDLGAKPFQVLRT
                     VILPLTSGGIFASIILTFIPALGIYIIADIYGGGKILYLGNLIRNQMLINRNWPFGCA
                     LSVLLIGITLLMLFVFTRFYNLEDLEV"
BASE COUNT          503 a          390 c          301 g          552 t
ORIGIN      
        1 acaccatcaa aagggaaaca cccaatatga tctccggaat tacgatcgga acatacataa
       61 tattcatcag cagctgcttt cctttaaatt tatatctggt aattccataa gctgccattg
      121 ttccgataat tacggaaatg atcgtactga agatcgaaac gatcagcgta ttggaaagga
      181 tcgtcatcag ctccttgtcc aaaaataact ttccgtacca ggaagtcgta aatccctgcc
      241 agattatgtt ctgcttctgt gtattaaaag aataaatgat catgactacg atcggcacat
      301 acagcagcag ataccacatt gaaattccaa accaggcaaa gccccttcca acagcatgtt
      361 ttcttcttct catctcaccg gatttcataa gccactccat tccgcgccgc catgtccgca
      421 tctgcaatgc agacctttgc gctgtttatt ttttctccta cacttccaga tcttccagat
      481 tataaaatct cgtaaagaca aacagcatca gcagtgtaat accgatcagc aatacggata
      541 aggcacagcc gaacggccag ttcctgttga tcagcatctg atttcggatc aaatttccaa
      601 gatacaggat tttcccgccg ccgtaaatat ccgctatgat atagatccca agtgccggaa
      661 taaacgtcag aataatactt gcaaaaattc cgccgcttgt cagcggaagg atcactgtcc
      721 gcaatacctg aaacggtttt gcccccagat cctttgacgc ctccaaaaaa gaatcgtcaa
      781 gcttttcaat agagctgtac agcggaagaa tggaaaaagt catcagactc aatatcgtcc
      841 cgaatgcaac cgctccgtta ttgtacaaaa actgtacggg tgaatcgata agcttaagcc
      901 actgcagcaa tccgttcaaa atacctttgt tctgtaaaat acgtacccag ctgtacgttc
      961 ttacaagacc gatcgtccat gacgggatca tcatcagcat gatcatatag ccgcctctgc
     1021 tccctttttg ctttgctata aaataagcga tcggatagct gatcagcaga accgttattg
     1081 tggaaatgat cgctatcacg atcgtaatgc cgaaaattct taagtaggta ggctgcagca
     1141 tttccgtata attttcagtt gtcagctggt actggatgta accataatct ccctgactca
     1201 taaaactgat cacaatgaca tagatcatcg gaattacgat aaaagccaaa agccacagcg
     1261 caattggtcc gaccgttatg atcgccggta tcgtatttgt cttgaactgc ttctttttca
     1321 aaaatttctg tcgttccgta tttctctgac tcagctttgt ttcagtcatc tggatccctc
     1381 ctgtcttttt atcatttaca gctttttaat gatagaaaaa agccgtctta aactgcaaga
     1441 cgactttgtc caatctgaat tttaaaattt aatttaacta tggttaatat taaagcacac
     1501 tttcttttgc gtgtcaacaa ttattttttt ttattttcgc cggtttttat taaaatttga
     1561 aagcacttaa gaacacagcg tctgggtact atttatttct gcttttcaat gtatcttttt
     1621 tactgttgtg gttaattctt ctttgctttt tttttacggt ttcctaaaga tagttatttt
     1681 gaacatgatt ggttttggga gtgtattttg aaaaaaatta ttattgtttt acttgttttc
     1741 cttttt
//
LOCUS       DAOSYE010000522         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58661_length_1745_cov_3.588757, whole genome shotgun sequence.
ACCESSION   DAOSYE010000522 DAOSYE010000000
VERSION     DAOSYE010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58661_length_1745_cov_3.588757"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLU43_08790"
     CDS             <1..305
                     /locus_tag="PLU43_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO28548.1"
                     /translation="DTYLQSYMGLDEESFNEKVQEASQTIAKQSLVLQAIADAEEIEI
                     TDEELEQKVNDLVDLYGYESEKDLKEHMSVDDIKESMLIKKVEEFLADHAVITVSE"
     gene            339..1730
                     /gene="asnS"
                     /locus_tag="PLU43_08795"
     CDS             339..1730
                     /gene="asnS"
                     /locus_tag="PLU43_08795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOO28549.1"
                     /translation="MDLISIKELYKNREQYFEKEIIVGGWIRSLRDSKTFGFIVINDG
                     TFFESLQIVYDEGLSNFAEISRMNVGAALVVRGMLIATPHAKQPFEIQASEIIIEGTS
                     EPDYPLQKKRHTVEYLRTIPHLRARTNLFQAVFRIRSMTAYAIHRFFQERDFVYVHTP
                     LITGSDCEGAGEMFRVTTLNMDELPKRETGSIDYGKDFFGRETKLTVSGQLNGETYAM
                     AFKNIYTFGPTFRAENSNTPRHAAEFWMIEPEMAFADLNDDMMLAESMIKYIIRYILE
                     NAPEEMQFLNRFADQGLLERLQHVLASDFAQVTYTEAVGILQKHNDRFEYKVSWGCDL
                     QTEHERYLTEQEFKRPVFVTDYPKEIKAFYMKLNEDGRTVAAMDCLVPGIGEIIGGSQ
                     REDDYEKLLARIDELGFSKEEYAFYLDLRRYGSARHAGFGLGFERCVMYLTGMSNIRD
                     VIPFPRTVNNCEL"
BASE COUNT          524 a          313 c          481 g          427 t
ORIGIN      
        1 ttgatacata ccttcaaagt tatatgggac tggatgagga aagctttaac gagaaggtgc
       61 aggaagcttc acagacgatt gcaaagcaga gtcttgtgct gcaggcgatc gccgatgcgg
      121 aggaaatcga aataacagat gaggaactgg aacagaaagt gaatgatctg gtcgatcttt
      181 acggatatga atcggaaaag gatctgaaag aacatatgag tgttgacgat atcaaagagt
      241 ccatgctgat caagaaggtg gaagaatttc ttgcggatca tgcggtcatt acggtaagtg
      301 aataaaaaca ggagagcatg aaaggcagga agagaagaat ggatctgatt tcgataaaag
      361 agctgtataa aaacagggaa cagtattttg agaaggagat catagtcgga ggatggatca
      421 gaagtctgag agattccaaa acatttgggt ttattgtaat caatgacggg actttttttg
      481 aatcactgca gatcgtatat gacgaaggac tttccaattt tgccgagatt tcaaggatga
      541 atgtcggtgc ggcacttgtt gtcaggggga tgcttattgc gacgccgcat gcaaagcagc
      601 catttgagat ccaggcatct gagatcatca tagagggaac gtctgagccg gattatccgc
      661 tgcagaaaaa gagacataca gtggagtatt taaggaccat tccgcatctg cgggcgagaa
      721 ccaatttgtt tcaggcagtt tttcggatcc gttccatgac cgcatatgcc attcatcgtt
      781 tttttcagga acgggatttt gtatatgtgc atacgccgct gatcacgggc agtgattgtg
      841 aaggcgccgg agaaatgttc cgcgtaacga cactgaacat ggacgaactt ccgaaaaggg
      901 agaccggcag cattgactat gggaaagact tttttggcag ggaaacaaag cttaccgtaa
      961 gcggacagct caatggcgaa acatatgcaa tggcttttaa aaatatttat acattcggac
     1021 cgacctttcg ggcggaaaat tccaatacgc cccgccatgc ggctgagttc tggatgatcg
     1081 agccggaaat ggcatttgca gatctgaacg atgatatgat gcttgcagaa agcatgatca
     1141 aatatattat tcgatatata ttggaaaatg caccggaaga aatgcagttt ttaaaccgat
     1201 ttgcggatca gggacttttg gagagactgc agcatgtact tgcttccgat tttgcacagg
     1261 tgacatatac ggaggcagtg gggattttac aaaagcataa cgaccggttc gaatacaaag
     1321 tatcctgggg ctgtgacctg cagacagagc acgagcgcta cctgacggag caggaattta
     1381 aacgcccggt atttgtaacg gattatccga aagaaatcaa ggctttttat atgaagctga
     1441 acgaagacgg cagaacggtc gcggccatgg actgcctggt gcctggaatc ggggagatca
     1501 tcggcggaag tcagagagag gacgactatg aaaagctgct tgcaaggata gacgagctgg
     1561 gattttcaaa agaggagtat gctttttatc tggatctgcg caggtatgga tctgcgaggc
     1621 atgcgggctt tggtcttggg tttgaaagat gcgtcatgta tctgaccgga atgtccaata
     1681 tccgtgatgt cattccgttt ccaagaacgg taaataactg tgaattataa tgcagcacgg
     1741 agttt
//
LOCUS       DAOSYE010000523         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58866_length_1742_cov_2.054535, whole genome shotgun sequence.
ACCESSION   DAOSYE010000523 DAOSYE010000000
VERSION     DAOSYE010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58866_length_1742_cov_2.054535"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..360
                     /locus_tag="PLU43_08800"
     CDS             121..360
                     /locus_tag="PLU43_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28550.1"
                     /translation="MYRKSVRQPAPVASAVLDGDEAHAVLLDANFDEDWGDKAYMQTI
                     AHHIKNEKHTLEIKLTNVVENNQVPFYLINVISSC"
     gene            400..>1742
                     /locus_tag="PLU43_08805"
     CDS             400..>1742
                     /locus_tag="PLU43_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOO28551.1"
                     /translation="MGISYNEKEMIWKLDTPHASYIICAADTERFLLHAYYGKKISDD
                     AVGYLTRIYEPPFVPSVNNRDRGAFLDAAPMEYPTYGVGDFRGAALEIETQEGHRGLS
                     LSYKSHQIYAGKPELAGLPATFGSKKECTTLELTVSDNVIGLEVVLVYTVFEQLDVIT
                     RSVRVKNCGEKALYLKRVLSACIDMDEQEFDLITLHGAWARERHIYRRKIGPGTQAVS
                     SVRGESSHQEHPFMAAAGRHTTQDTGEIYGFNFVYSGNFLAQCETNQFDSIRMLMGIN
                     PSGFSWKLESNAEFIAPEVVMVYSADGIDRMSQTFHDLYRGHLIRSAYQDKKRPVLIN
                     NWEATYFDFDTDKLIAIAKEAGKHGIEMLVMDDGWFCGRSSDNMALGDWYVNEEKIRG
                     GLKHLVDEVNSLGMEFGIWIEPEMISPDSDLYRAHPDWAIQIPGRTPTLLRNQYVL"
BASE COUNT          469 a          342 c          489 g          442 t
ORIGIN      
        1 acatatgtac atttttattt aatactttct gtacatagat aagtataact ttatagctgg
       61 tatgcggaca gagaaggcgc tcgtattctg tttgaactgt atggcagtga aatagcagtc
      121 atgtacagaa aatccgtacg gcagccggcg cctgttgcca gtgcggttct tgatggcgat
      181 gaggcgcatg cggtcctgct tgatgcgaat tttgacgagg actggggcga taaggcatat
      241 atgcagacga tcgcgcatca tatcaaaaac gaaaaacata cgctggaaat aaaactgacc
      301 aatgtggtcg aaaataatca ggtgccgttt tacctgatca atgtaatttc atcatgttag
      361 cttcggatag gttttgtgta tccgatgaaa ggaaacgata tgggaatcag ttataacgaa
      421 aaggaaatga tatggaagct ggatacaccg catgcttctt atattatctg tgcggccgat
      481 acggaacggt ttctgctgca tgcctactat ggaaaaaaaa tcagtgatga tgcggtcggc
      541 tatcttacga ggatctatga gccgcccttt gtcccaagtg tcaacaacag ggatagaggt
      601 gcctttttag atgcggcgcc gatggaatac ccgacttacg gagtcggtga ttttcgcggg
      661 gcggccctgg aaatagagac acaggaagga caccgcggac tgtcgctttc ttataagagt
      721 catcagattt atgctggaaa gccggagctt gcgggactgc ctgcaacgtt tggcagcaaa
      781 aaggagtgta cgacgcttga attgaccgtt tcggataatg ttattggtct ggaggttgtt
      841 ttagtctata cggtatttga acagctggat gtaatcacaa gaagtgtgag agttaaaaac
      901 tgcggggaga aggcactgta tttaaaacgt gtgctgtcgg catgcatcga tatggatgag
      961 caggaatttg atctgatcac cctgcatggt gcgtgggcca gagaacgcca tatctacagg
     1021 agaaagatcg gtccgggcac acaggcggtg tcatccgtac gcggggaatc aagccatcag
     1081 gagcatcctt ttatggcagc tgcaggcagg catacgacgc aggataccgg ggagatctac
     1141 ggttttaatt ttgtatattc agggaatttt cttgcgcagt gtgagacgaa tcagtttgac
     1201 agtatccgga tgctgatggg gatcaatccg tcgggatttt catggaagct tgaaagcaat
     1261 gcggagttta ttgcaccgga agttgtcatg gtctattctg cggatgggat cgaccgtatg
     1321 agtcaaacat ttcatgacct ataccgtggg catttgatcc ggagtgccta tcaggacaaa
     1381 aagcgccctg tcctgatcaa taactgggaa gctacttatt ttgattttga tacggacaaa
     1441 ttgatcgcga tcgcaaaaga ggccggaaag catggcattg agatgctggt catggacgac
     1501 ggctggttct gtggcagaag cagcgacaat atggcgcttg gagactggta tgtcaatgaa
     1561 gagaagatca ggggaggctt aaagcatctg gtggatgaag tcaacagcct cggtatggaa
     1621 ttcggcatct ggatagagcc ggaaatgatc tctccggatt ccgatctgta tcgtgcacat
     1681 ccggactggg cgatccaaat accgggccgt acgccgacac tgctccggaa ccagtatgta
     1741 ct
//
LOCUS       DAOSYE010000524         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58991_length_1739_cov_5.247625, whole genome shotgun sequence.
ACCESSION   DAOSYE010000524 DAOSYE010000000
VERSION     DAOSYE010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58991_length_1739_cov_5.247625"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLU43_08810"
     CDS             <1..367
                     /locus_tag="PLU43_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOO28552.1"
                     /translation="SLKKSGKTIILATSKPEEFANEILKYFSLDKYFDFIAGATMDGA
                     RCKKAEIIKYALNCNGITELEKVIMVGDRKHDIFGANQIGIDSVGVLFGYGSNEELRK
                     SGATYIAESVEDIMKYMND"
     gene            525..>1739
                     /locus_tag="PLU43_08815"
     CDS             525..>1739
                     /locus_tag="PLU43_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO28553.1"
                     /translation="MQRLSKKSRLLETYSLIIIDVLGILISYAGSLFLRFRGEAAFKQ
                     IHYSVGAYLLIIALLYSVMTEWNRGFFDRGSLVELIAIIKYNIVLLLAVSSLVFLVKA
                     DGFSRLVFGYFAVFNTVITYILHLLFKKYMLEHYRNSASSDKVMVVTERLYADRLMKK
                     ILTEKAWNYAITAIAILDEEKAAGEEKIQGIPVVAGESDLFERARQMPLDEVFLYLPG
                     MPIEAVKRTIQDFETMGVTCHFNVEIPELNLDGKTAGNFAGFAVMTFSLQYLDYRRMF
                     VKRGFDILGSLVGLAITAVLTPFVALAVKLESKGPVFFSQVRIGKNGRRFKIWKFRSM
                     YVDAEQRKKELEKQNEMQGLMFKMKDDPRITKVGRVIRKLSIDELPQFYNILKGDMSL
                     VGTRPPTTDEFER"
BASE COUNT          526 a          280 c          448 g          485 t
ORIGIN      
        1 tagtttgaaa aaatcaggaa aaacaataat acttgcgacc tctaagccgg aagaatttgc
       61 aaatgaaata ttaaaatact tttcattaga taaatatttc gattttattg caggtgctac
      121 gatggatgga gcacgatgca aaaaggctga aataataaag tatgctttaa attgcaacgg
      181 aataacagaa ttagaaaagg tgataatggt tggtgacaga aaacatgata tattcggagc
      241 aaatcaaatc ggcatagact ctgtaggggt gctttttgga tatggaagca atgaagaatt
      301 gcgcaaatca ggagcaacct atatcgcaga atctgtggaa gatattatga aatatatgaa
      361 cgattagatt taaaagatat gaaagcgaat tgcgatttgt cgggagaatg caataatagt
      421 cagcttattt gataaagctg ccggcagggt tttggaggaa atttcggtag cggatcaggg
      481 tggttcgtgt tatactgaaa caaaatgcgg aaaggaaaca gatcatgcag cgattgagta
      541 aaaagagccg tctgctcgag acatattctt tgattattat agacgttctt ggcatactga
      601 tttcttatgc aggctctctt tttctgcgtt tcagagggga agcggccttt aagcagatcc
      661 actattccgt tggggcctat cttttgatca ttgcgctttt gtacagtgtg atgacagagt
      721 ggaacagagg cttttttgac agaggcagtc ttgtcgagct gattgcgatc atcaaatata
      781 atatcgtact gcttttagct gtcagcagtc ttgtatttct tgtaaaggca gatgggtttt
      841 cccgtcttgt gttcggctat tttgcagttt tcaatacggt tatcacctat attctgcatc
      901 ttttatttaa aaaatatatg ctggagcatt accgcaacag tgcgagcagt gataaggtaa
      961 tggtcgttac cgaaagactt tatgccgacc ggctgatgaa aaaaatactg actgaaaaag
     1021 cctggaatta tgcgattaca gccattgcta ttttagatga agagaaggcg gcaggagaag
     1081 agaagatcca ggggatccca gttgtggcgg gagagtcgga tctgtttgag agagcgcggc
     1141 agatgccgct tgatgaagtg tttctctacc tgcccggcat gccgatcgaa gcagtaaaaa
     1201 gaacaataca ggattttgag acaatgggag tgacctgtca tttcaatgtg gagatcccgg
     1261 aactgaatct ggatggaaaa acagcgggta attttgccgg ttttgcggtt atgacgtttt
     1321 ccctgcagta tctggattac agacggatgt ttgtaaaaag aggatttgat attttaggga
     1381 gtctggtcgg actggcgatc actgcggtac tgactccgtt tgtggcactg gctgttaaac
     1441 ttgaatcaaa aggaccggtc tttttctcac aggtcaggat cggaaaaaac ggccgtcgtt
     1501 ttaagatatg gaagtttcgt tccatgtatg tggatgcgga acagcgaaaa aaggaactgg
     1561 aaaaacagaa tgaaatgcag gggctgatgt ttaaaatgaa ggatgatccg cggatcacaa
     1621 aggtcggcag ggtcatacgc aagttaagca ttgacgagct tccgcagttt tataatattt
     1681 taaaaggtga tatgagcctt gttgggacga gaccgcctac gacagacgaa tttgaacgg
//
LOCUS       DAOSYE010000525         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_58999_length_1739_cov_3.740499, whole genome shotgun sequence.
ACCESSION   DAOSYE010000525 DAOSYE010000000
VERSION     DAOSYE010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58999_length_1739_cov_3.740499"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1354)
                     /locus_tag="PLU43_08820"
     CDS             complement(86..1354)
                     /locus_tag="PLU43_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO28554.1"
                     /translation="METYKFLLDLGVILLSTKLLGLLTRRLNMPQVVGALLAGLLLGP
                     AMLGIITQTEYIQETAEEGVVVLMFCAGMETDVEELKKSGKSAFIIALIGVIVPLIGG
                     YLVAWFFNKPGMIESDAAASIFLQNMFIGVILTATSVSITVETLKELGKLKTNSGNAI
                     LGAAVIDDILGIIALTIITSMTDSSVKISLVLLKIVGYFIFAGVAGFLFYKFYAKWSN
                     NSKKGLQRHTIIAFVFCLLMAYVAEKFFGVADITGAYIAGLIISMTQKEQYLASRFDI
                     ISYLYLSPIFFASIGLKVNLPAMSMKVVAFSVILVVVAILTKVIGCGLGAKLCGYKNY
                     QSIRIGVGMISRGEVALIVASKGAALGLMGSDFMGPVVIVVILTTIVSPILLKIVFKN
                     NGSTDIPLVTEGFSDNYEEIDQYREGEKHV"
     gene            complement(1378..>1739)
                     /locus_tag="PLU43_08825"
     CDS             complement(1378..>1739)
                     /locus_tag="PLU43_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28555.1"
                     /translation="ILIVLAGFLSFERIWDKESIGKPLFRDFLLVINRDYYAEPDPDQ
                     SVYFMYDFSNYSGHLMDPITDTASLHTLFTGLIPAITCLCFFLFFTGACLDRKNYIIR
                     FIHNADGSKWKNGYCLS"
BASE COUNT          539 a          439 c          312 g          449 t
ORIGIN      
        1 tctgccgccc ccagcggact gcccgtatac aagagccggc ggtcatttaa aacccgccgg
       61 ctcaaatctg atcggaaatt tttatttata catgcttttc gccttcgcgg tactggtcga
      121 tttcctcata attgtcggaa aatccttccg tcacaagagg tatatcggta gagccgttat
      181 ttttaaatac gatctttaac agaatcggcg aaacaatggt cgtcagtata acaacaataa
      241 caaccggtcc cataaaatca cttcccatga gtccaagtgc ggctcccttg cttgccacga
      301 tcaaagccac ttcccctcgg gatatcatac cgacaccgat cctgatcgac tggtaatttt
      361 tatagccgca cagcttcgcg ccaagtccgc agccgataac cttcgtcagt atcgcgacga
      421 cgaccagaat caccgaaaat gcaactactt tcatgctcat ggccggcaga ttgactttca
      481 gaccgatact tgcaaagaag atcggggaaa gatacaaata ggagatgata tcaaaacgcg
      541 aggccagata ttgttccttc tgagtcatgg agataatcaa acctgcaata taagcgcctg
      601 taatgtctgc aacaccaaaa aacttttcgg ctacatatgc catgagcaga caaaacacaa
      661 acgcaatgat cgtatgcctc tgcagaccct ttttagaatt attgctccat tttgcataaa
      721 atttatagaa caggaaaccg gcaacccctg caaaaataaa gtatcctact atttttagca
      781 ataccaggct gatcttgacc gaactgtcgg tcatactggt tatgatcgtc aacgcaataa
      841 taccaagaat atcgtcgata acagccgctc ccaaaatagc attaccggaa ttggtcttca
      901 gcttcccaag ttcttttagc gtttcgaccg taatactgac ggacgtggcc gttaaaatta
      961 caccaataaa catattctgc agaaaaatac ttgctgcagc atccgattcg atcattccgg
     1021 gcttgttgaa aaaccatgca accagatatc cgccgatcag cggaacaatt acaccgatca
     1081 gcgcaattat aaacgctgat ttcccgctct ttttcaattc ctctacatcc gtttccatac
     1141 ctgcgcagaa catcaggact acaacgccct cctcagcagt ttcctgaata tattccgtct
     1201 gcgtaatgat accgagcatc gccggtccaa gcagcagtcc tgcaagcagt gcgccgacta
     1261 cctgcggcat attcagccgg cgggtaagca ggcccaacag ttttgtactg agaagtatca
     1321 cacctaaatc caataaaaat ttataagttt ccattaataa gcctccattc tttttcttta
     1381 tgataaacag taaccattct tccatttact gccgtcagca ttatgaataa aacgaataat
     1441 ataattttta cggtctaaac aggctcctgt aaaaaacaga aaaaaacaga gacatgttat
     1501 cgcaggaatc agaccggtga ataacgtgtg taaagaagcc gtatctgtta tcggatccat
     1561 caggtgaccg ctgtaattag aaaaatcata cataaaataa acgctctggt ccggatccgg
     1621 ttccgcataa taatctctgt ttatgacaag cagaaaatcc ctgaataacg gttttccaat
     1681 actttctttg tcccatatcc tctcaaagct caaaaaacct gctaaaacaa ttaatataa
//
LOCUS       DAOSYE010000526         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59180_length_1736_cov_3.158834, whole genome shotgun sequence.
ACCESSION   DAOSYE010000526 DAOSYE010000000
VERSION     DAOSYE010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59180_length_1736_cov_3.158834"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..832
                     /locus_tag="PLU43_08830"
     CDS             134..832
                     /locus_tag="PLU43_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOO28556.1"
                     /translation="MRLQRLLMIIFYLKMHNFATTAELSEYLEVSKRTISRDIEILRT
                     AGVTIESTTGKNGGYLLAEDFSFDSIIFSKSELSSMLLGTQFLYQFKNTEFAREAELL
                     SDKISKLLEGNKEPDIKAREFLLIDTETNPRAEYNINAILQQIENAYDSRKLLFIDYK
                     SMMCHKFSTSGLVAPYGLVNKSGFWYLAGYCYEHQAYRVFNIAFIQNIYITQDSFERD
                     RTFHLDAFWETQKY"
     gene            855..1010
                     /locus_tag="PLU43_08835"
     CDS             855..1010
                     /locus_tag="PLU43_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28557.1"
                     /translation="MEIKYVQNGEKGRTCADCKHFERDVNMQGAGKCFGNEVSAEGGC
                     NFFEARD"
     gene            complement(1070..1444)
                     /locus_tag="PLU43_08840"
     CDS             complement(1070..1444)
                     /locus_tag="PLU43_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28558.1"
                     /translation="MELSFNIVIERQLVQAIIVSLTLVIVIMILLYSLVIMMTAEEKR
                     LHFYGMSARGAVGTASSLIFSVLMAHINLREQFSGEVAYLEMFYYIAYLYILVSSVLI
                     YIFMKNFENKNHWLVITFLWLR"
     gene            complement(1509..>1736)
                     /locus_tag="PLU43_08845"
     CDS             complement(1509..>1736)
                     /locus_tag="PLU43_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28559.1"
                     /translation="WCSLDAVPTCKIGLYVLSLKFTSSNDVEVTGYVWQTINKKDLKQ
                     KSFGISFPQAVGESVKDEQYQTETEDTVTKG"
BASE COUNT          601 a          276 c          364 g          495 t
ORIGIN      
        1 gagatttaaa acgaatggca gcagagaaaa agaggtcata ataggggaat taaaaaaact
       61 tattggatga aaaatttaat actgccatac aggtggcaat attaatagtg tatactaatg
      121 acaggtgaag gaaatgagat tacaaagact tttaatgatt atcttttatc tgaaaatgca
      181 taattttgct acgactgcgg aattatcaga atatctggaa gtttcaaagc ggacgatcag
      241 ccgggatatt gagatactta gaacagccgg agttacaatt gaaagcacta ctggaaaaaa
      301 tggaggctat cttctggcgg aagatttttc ttttgacagt attattttca gtaagtcgga
      361 attaagttcg atgctgttag ggacgcaatt cctgtatcag ttcaaaaaca cggaatttgc
      421 cagggaagca gaactgctaa gtgacaaaat ttccaaactg ctggaaggca ataaagaacc
      481 ggatataaaa gccagagaat ttttattgat agataccgaa accaatccaa gggcggaata
      541 taatatcaat gcgatcctgc agcagataga aaatgcgtat gacagcagaa agcttttatt
      601 tatagattat aaaagtatga tgtgtcataa gttttcgaca tcaggacttg ttgctccgta
      661 tggtctggta aataagtcgg gattttggta tctggcagga tattgttatg aacatcaggc
      721 atatcgggta tttaatattg catttattca aaatatttat ataacgcagg attcctttga
      781 aagagaccgt acatttcatt tggatgcttt ttgggaaact caaaaatatt aataatagaa
      841 tgggaggtga tgatatggag atcaagtatg tacaaaatgg tgaaaaaggc agaacctgcg
      901 cggactgcaa acattttgag cgcgatgtaa atatgcaggg agccggaaaa tgttttggaa
      961 atgaagtatc tgctgaagga ggctgtaatt tttttgaagc aagggattaa aacaagagaa
     1021 gcaacatcta aagagcagct ggtatggctg ctcttacagt atttgccgct taccttaacc
     1081 agagaaaagt aataacaagc caatgatttt tattttcaaa atttttcata aaaatataga
     1141 tcaggacaga actgactaat atatagagat aggctatata ataaaacatc tccaagtagg
     1201 caacttcacc tgaaaattgc tcgcgtaagt ttatatgtgc cattaatacg gaaaagatca
     1261 gagaggatgc ggtgccgact gcgcctcgcg ccgacatgcc ataaaaatgt aatctttttt
     1321 cttctgccgt catcataatg accagactat aaagtagtat cataataaca ataacgagtg
     1381 tcaggctgac aataatagcc tggacaagct ggcgttcgat aacgatgtta aaagataatt
     1441 ccatatggtt ttatgatcga gcggatattt ggaatagtca aatgactgac gaagatcgtt
     1501 tcaaaatact atccttttgt aaccgtatct tctgtctcag tctgatattg ttcatccttt
     1561 actgattcgc cgacagcctg cggaaaagaa atgccgaaag acttctgctt cagatctttt
     1621 ttgtttatcg tctgccagac atatcctgta acctctacat cattactgga agtgaatttt
     1681 aatgataaaa cataaagtcc tattttacaa gtgggaacgg catccaaaga acacca
//
LOCUS       DAOSYE010000527         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59187_length_1736_cov_2.963117, whole genome shotgun sequence.
ACCESSION   DAOSYE010000527 DAOSYE010000000
VERSION     DAOSYE010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59187_length_1736_cov_2.963117"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLU43_08850"
     CDS             complement(<1..704)
                     /locus_tag="PLU43_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOO28560.1"
                     /translation="MSNTSQASARINSLLDENSFVEIGGLVTARATDFNMEPKETPAD
                     GVVTGYGLIDGNLVYVYSQDASVMGGSVGEMHAKKIVNLYDFALKMGAPVIGLLDSAG
                     LRLQEATDALNAFGEIYKKQVLASGVIPQITAVFGSCGGGLALFPAMADFTFMEEKKA
                     KLFVNSPNALDGNIESKNDTAAAKFQSEASGIVDAVTDEEGIYAQIRQLIMMLPSNNE
                     ENGSFDECTDDLNRVSA"
     gene            complement(927..1097)
                     /locus_tag="PLU43_08855"
     CDS             complement(927..1097)
                     /locus_tag="PLU43_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOO28561.1"
                     /translation="MLEIILLIILALWFYLAVKKLYKDRKNGRCSGCSSANCCTNCGH
                     GKQHEEDNKREP"
     gene            complement(1136..>1736)
                     /locus_tag="PLU43_08860"
     CDS             complement(1136..>1736)
                     /locus_tag="PLU43_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOO28562.1"
                     /translation="VALLLKNTLFKGNPIPFVMELPNYRLPSAKSVLLLMWEKAKDFL
                     QKAFTVIFVATIVIWFLQTFDLKLNVVSDSADSILAMVGQWIAPVFALCGFNDWRIVT
                     SLISGFTAKEAVVSTMSVLLNVGTAELKNALPALFTPLSAVSFLLFTLLYTPCVAAVA
                     TMKQELKSGIETLGIVIAQCTVAWIISVLVFQIGSFLIG"
BASE COUNT          515 a          430 c          297 g          494 t
ORIGIN      
        1 gcgcttactc tgtttaaatc atccgtacac tcgtcaaaag aaccattctc ttcattattt
       61 gaaggaagca tcatgatcaa ctgtctgatc tgtgcataga tcccttcttc atcggtaaca
      121 gcatctacaa taccgcttgc ttcactctga aacttagctg cagccgtatc attttttgat
      181 tcgatattgc cgtcaagcgc attcggagaa tttacaaaca gctttgcttt tttctcttcc
      241 ataaatgtaa aatcagccat tgcaggaaat aatgcaagtc cgccgccgca gcttccgaac
      301 actgccgtaa tttgcggaat aacgccggat gcaagcacct gtttcttata aatctcaccg
      361 aaagcgttca aagcatctgt tgcttcctga agccttagcc ctgcggaatc aagcagacca
      421 atgacaggag ctcccatttt caaagcaaaa tcgtataaat taacaatttt ctttgcatgc
      481 atttcaccaa cagaaccacc cattacagaa gcgtcctggc tgtaaacata tactaaatta
      541 ccgtcaataa ggccatagcc tgttacaaca ccatctgccg gagtttcctt cggttccatg
      601 ttgaaatcag ttgctctggc tgttactaaa ccgccaattt caacaaaact gttttcatcg
      661 agcaaagaat taattcttgc actcgcttga gaagtattac tcattttgtt cagccctcca
      721 ttttatataa attatttatt agacagtttt cgcacgtcca cgcatcgtta agattataac
      781 acaactcccc tgcaaagcat agtaataaat caaagaaatt tttgtgaatt tttcgtaaga
      841 tttctttttt ttcatcattt ctttttcaat tatccgaata ctaggaaccc gtgaggaata
      901 ttgtataaac accatatggc atcaatttaa ggttcccttt tgttgtcctc ttcatgctgc
      961 ttcccgtgcc cgcagtttgt acagcagttc gccgaggaac agccggaaca tctgccgttt
     1021 tttctgtctt tatatagttt ttttaccgcc agataaaacc acagcgcaag tatgataaga
     1081 agtatgatct ccagcatata aaccacctcc ggttctgcgc tttataaaca ctccttcagc
     1141 ctatcaaaaa actgccgatc tgaaatacca gtacggaaat gatccatgca accgtacact
     1201 gtgctattac aataccaagt gtctcaattc cggatttcag ctcctgcttc attgtagcta
     1261 ctgccgcaac acaaggcgta taaagcagcg taaataataa aaaactgaca gcggaaagag
     1321 gcgtgaataa agcgggcagc gcatttttaa gttcagccgt tcccacgttc aaaagtacac
     1381 tcattgtact gacaacagct tctttggcag taaatcccga aatcagggaa gtcacgatcc
     1441 tccagtcatt aaatccgcat aatgcaaaca ccggcgcaat ccactgccca accatcgcaa
     1501 ggatactgtc cgcactgtca ctgacaacgt ttagttttaa atcaaacgtc tgcagaaacc
     1561 agatcacgat cgttgccacg aaaataacgg taaacgcttt ctgcagaaaa tcctttgcct
     1621 tttcccacat aagaagcagc acgctttttg cagaaggaag ccggtaatta ggcagttcca
     1681 taacgaatgg aataggattt cccttaaaca gcgtattctt cagcagtaaa gcgacc
//
LOCUS       DAOSYE010000528         1733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59340_length_1733_cov_2.756853, whole genome shotgun sequence.
ACCESSION   DAOSYE010000528 DAOSYE010000000
VERSION     DAOSYE010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59340_length_1733_cov_2.756853"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1354
                     /locus_tag="PLU43_08865"
     CDS             71..1354
                     /locus_tag="PLU43_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOO28563.1"
                     /translation="MKPTKQIKKDMRDGVYRELLKDIYEDDRVLDYQEARYAKAIEKF
                     EELYGEQEVEIYSAPGRSEIGGNHTDHQHGQVLAAGINLDAIAIAAKTEDKQIKIVSD
                     HFDIKPVDLSDLEKKEEETGTSEALVRGVAYKLQKEGFKLGGVYVYMTSDVLVGAGLS
                     SSAAFEVAVCTVISGLYNEMKISSILSAQVSQYAENVYFGKPCGLMDQMASSVGSLVN
                     IDFKDTKDPVVNRLDVDFGRFAHSLCIVDTKGDHTDLTNEYAYVPQEMKKVAAYFDCE
                     YLREVDEEQFYRELPNVRKTAGDRGVLRAIHFFGDNRRVGEEVEALQNGEFEKFKELV
                     TESGDSSYKYLQNVFANCDVQNQSVSLGLALSERILGKKGACRVHGGGFAGTIQAFVP
                     DELVAGYKEQMEYFFGKDSCHVLKIRKYGGMKVLG"
     gene            1383..>1733
                     /locus_tag="PLU43_08870"
     CDS             1383..>1733
                     /locus_tag="PLU43_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO28564.1"
                     /translation="MKVLVLGGAGYIGSHTVYALIEAGEQVVIIDNLLTGYEAAVHPQ
                     AVFYKGDIRDRTFLDKVFEKEKDIDAVIHFAASSLVGESMTDPLKYYENNLYGTMVLL
                     GALVAHGIDKVVFSS"
BASE COUNT          538 a          295 c          481 g          419 t
ORIGIN      
        1 atttactaaa catatcacga aaaacaaaat gtttagtaaa caaaccagca aaatataagg
       61 gagaagatca atgaagccga caaaacaaat aaaaaaagac atgcgagacg gagtgtatcg
      121 ggaattactg aaagacattt atgaagatga cagggttttg gattatcagg aagcccgtta
      181 tgcaaaagca atcgagaaat ttgaggaact ttatggcgag caggaggtcg agatctacag
      241 tgcgcccggg cgcagtgaga tcggcggcaa tcatacggat catcagcacg gtcaggtgct
      301 ggcggcaggc attaatctgg atgcgatcgc aattgccgca aagacggaag acaagcagat
      361 caagatcgtt tccgaccatt ttgatatcaa accggttgac ttgtctgacc ttgaaaagaa
      421 agaagaagag accgggacaa gcgaagcact ggtcagggga gttgcgtata agctgcagaa
      481 agagggtttt aaactcggcg gtgtttatgt ctatatgaca agcgatgtcc tggtaggggc
      541 cggactttcc tcctcggcag cctttgaggt ggcagtatgt acggtgattt ccggattata
      601 taatgaaatg aaaatcagtt cgattttaag tgcacaggtc agccagtatg cggaaaatgt
      661 atatttcggc aagccgtgcg gattgatgga tcagatggca tcgtcggtcg gcagtcttgt
      721 aaatatcgat tttaaagaca cgaaagatcc ggtcgtcaac cggcttgacg tagattttgg
      781 caggtttgca cacagtctgt gcatcgttga tacaaaggga gaccatacgg atctgacgaa
      841 tgaatatgct tatgtgccgc aggaaatgaa aaaggtggca gcatattttg actgtgaata
      901 tttgcgggag gtagatgaag aacaatttta cagggaactt ccaaacgtca gaaaaacagc
      961 cggagacaga ggcgttttgc gtgccattca tttttttgga gacaacagac gcgttggaga
     1021 ggaagtggag gcgcttcaga acggtgagtt cgagaaattt aaggaactgg tcacggaatc
     1081 cggggacagc tcttataaat atctgcagaa tgtatttgca aattgtgatg tgcagaatca
     1141 gagcgtttca ctggggcttg cactgagtga aaggatcctt gggaaaaaag gtgcctgccg
     1201 tgtacacgga ggaggatttg caggcacgat ccaggcattt gtgccggatg aactggtagc
     1261 cggatacaag gaacagatgg aatatttttt tggtaaggac agctgccatg tgctgaaaat
     1321 aagaaaatac ggcggcatga aggtgctggg ataaagcaga aacaaaagaa aggaaaaaaa
     1381 ttatgaaagt tttagtactg ggcggagccg gctatattgg ttcccatacg gtttatgcat
     1441 tgatcgaagc aggagagcag gtcgttatta tagacaatct tttaacgggt tatgaagcgg
     1501 ccgtgcatcc gcaggcagtt ttttataagg gagatatcag agacaggaca tttcttgaca
     1561 aggtatttga gaaggaaaaa gatatcgatg cggtcatcca ttttgcggca agctctcttg
     1621 taggggaaag catgacagat ccattgaagt attatgaaaa caatttatat ggtacaatgg
     1681 tattgttagg agcgcttgtt gctcatggaa tcgacaaagt ggtcttttcc tcc
//
LOCUS       DAOSYE010000529         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59451_length_1731_cov_3.172434, whole genome shotgun sequence.
ACCESSION   DAOSYE010000529 DAOSYE010000000
VERSION     DAOSYE010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59451_length_1731_cov_3.172434"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU43_08875"
     CDS             <1..886
                     /locus_tag="PLU43_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO28565.1"
                     /translation="GYPTYNFANVIDDHLMGITHVVRGNEYLSSSPKYTRLYEAFGWE
                     VPVYVHCPLITDEEHKKLSKRCGHSSYEDLLEQGFFSDAIVNFVALLGWSPEDNQEIM
                     SMDDLIRKFDYRHMSKSPAVFDYTKLKWMNGEYLKAMDFDEFFRLAKPYIKKTVTKEY
                     DLKKIAGLVKTRIEILPDIAEHIDFFEALPEYDIAMYIHKKMKTDAASSLEVLQELLP
                     LFEAQEDYCNDALYAVLSSYVQKKGCKNGYVMWPVRTAVSGKQMTPGGATEIMEILGK
                     EESVRRIKDGIDRLKRYL"
     gene            883..1611
                     /locus_tag="PLU43_08880"
     CDS             883..1611
                     /locus_tag="PLU43_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO28566.1"
                     /translation="MIQLNKEQTLAVRHADGPMLVLAGPGSGKTFVLTERIRFLIQEK
                     NIRSNEILVITFSKKAAEEMRQRFQKLCTNTYDQVRFGTFHAIFFHILQHYHNYQKES
                     ILTHTAKKEYIRNAAKHLDPDELYDDTWCEDMLYKISRYKNAGENAAESVAFADEKQT
                     EQFLTVYSLYQKQCHMENTLDFDDMIIKCYRLFAKNPNVCGEWRKIFQYILIYEFQDC
                     SLMQYEIIKLLAGEACNLFAVGDV"
BASE COUNT          538 a          312 c          399 g          482 t
ORIGIN      
        1 cggctatccg acctataatt ttgccaatgt gattgacgat catctgatgg ggatcacaca
       61 tgtggtaagg ggaaacgagt atttgtcttc ttctccgaaa tacacgcgtc tttatgaggc
      121 attcggctgg gaagtaccgg tttatgtgca ctgtccgctg atcactgatg aggagcataa
      181 aaagctttcc aaacgctgcg gccattcttc ttatgaagat cttctggaac agggattttt
      241 ttcggatgcg atcgtaaatt ttgtggctct tctgggatgg agtcctgaag ataatcagga
      301 gatcatgtca atggatgatc tgatcagaaa atttgattat cgccatatga gcaaatctcc
      361 ggcagtcttt gattatacaa agctgaaatg gatgaacggt gagtatttga aggccatgga
      421 ttttgacgaa tttttcagac tcgccaagcc ttacatcaaa aaaacagtca caaaggagta
      481 tgacctgaaa aaaattgccg gacttgttaa aacacggatc gagattttac cggatattgc
      541 ggagcatatt gatttttttg aagcattgcc tgagtatgac attgccatgt atatacataa
      601 gaaaatgaaa accgatgcgg cttcctcact tgaagtattg caggagctgc tgccgttgtt
      661 tgaagcacag gaggactact gcaatgatgc gctttatgcg gtattgtcat cctatgtaca
      721 gaaaaaagga tgtaaaaacg ggtatgtcat gtggcctgtc aggactgctg tttcaggaaa
      781 gcagatgacg cccggcggcg ctacggagat catggaaatt ctcggaaaag aggaatctgt
      841 caggcggatt aaggacggca tcgacagatt gaaacggtat ttatgataca attaaataaa
      901 gaacagacat tggctgtccg ccatgcggac ggaccaatgc ttgtacttgc cggaccgggt
      961 tccggcaaaa cattcgtctt gacagaacgg atacggttcc tcattcagga gaaaaacatc
     1021 cgttccaatg aaattcttgt tattaccttt tctaaaaagg ctgccgaaga aatgagacag
     1081 cgttttcaaa aactttgtac aaatacatat gatcaggtca gattcggaac atttcatgca
     1141 atattttttc atattttaca gcattatcat aactatcaaa aagaaagcat cctgacacat
     1201 acggccaaaa aagaatatat cagaaatgct gcaaaacacc tggatccaga tgaattatac
     1261 gatgacacat ggtgtgagga catgctttat aagatcagcc gttataagaa tgccggtgaa
     1321 aatgcggcgg aatccgtggc ctttgcagat gaaaaacaga cagaacagtt tttgactgtt
     1381 tattccttat accaaaagca atgtcatatg gaaaatacgc tggattttga cgacatgatc
     1441 ataaagtgtt acaggctctt tgcaaagaat ccgaatgtat gtggagaatg gcggaaaata
     1501 ttccaatata ttctgatcta tgaatttcag gactgcagtc tgatgcaata tgaaatcata
     1561 aagctgttgg caggagaagc ctgcaattta tttgcggttg gagacgtata aataacatat
     1621 gttattctac atatcaaaag gggtgcattt tataattgcc agaaagtgcc gtatattggg
     1681 catttctcgg cattggggta tgctgcaata gggcaaattc attgtattat g
//
LOCUS       DAOSYE010000530         1728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59644_length_1728_cov_2.624029, whole genome shotgun sequence.
ACCESSION   DAOSYE010000530 DAOSYE010000000
VERSION     DAOSYE010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59644_length_1728_cov_2.624029"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /locus_tag="PLU43_08885"
     CDS             <1..>1728
                     /locus_tag="PLU43_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO28567.1"
                     /translation="DVVDFVPNFDETETEPQVLPSRLPNLLINGAEGIAVGMVTSIPP
                     HNLGEVIEGVKAYMKNPSITVSELMTYIPGPDFPTGGVVTNKDELLSIYETGTGKIKI
                     RGKIEVENGKAGKNKIVISEIPYTMIGANISKFLMDIVSLVDTKKTADIVDISNQSSK
                     EGIRIVIELRRDADVENLKNLLIKKTKLEDTFGVNMLAVTDGRPETLNLKNIIRYHVN
                     FQREIATRKYRTLLNKELDRKEIQEGLIKACDVIDLIIEILRGSKDVKTAKACLMEGK
                     AEGIRFKSRISKKMAEQLCFTERQASAILEMRLYKLIGLEIEALMKEHNETLKNIETY
                     KDILTNPASMDHVIIRELDSYKKEFGRERRTRIENAAEIILEEKKLEEMEIMFLMDRF
                     GYAKTIDMQTYERNREAADAENKYVFSCMNTGRICIFTNTGQMHTIKVLDIPYGKFRD
                     KGTPIDNISNYSSQDETLIYICSLSDLVVQKLVFATKKAMLKHVYGTEFDVTKRTVAA
                     TKLAEQDEVLAVCPVTDQKNIVLQTKNSTFLKFSVDEIPLKKKGAIGVRGMKLSEDDE
                     LEAVYFLTNG"
BASE COUNT          576 a          312 c          410 g          430 t
ORIGIN      
        1 agatgtcgtt gactttgtgc cgaattttga tgaaacagaa acagaacctc aggtgctgcc
       61 ttccagactt ccgaatctgc tcatcaacgg tgcggaagga attgctgtcg gaatggtgac
      121 gagtatcccg ccgcataacc tcggcgaggt gatcgaaggc gttaaagcct atatgaaaaa
      181 tcccagtatt accgtttcgg aactgatgac ctacattccg ggtccggatt ttccaacagg
      241 cggtgtcgtc acaaataaag atgagctgct ttctatttat gaaacaggca ccggaaaaat
      301 aaagatccgc ggaaaaatag aagtagaaaa cggaaaagcc ggaaaaaata aaattgtcat
      361 ttcggaaatc ccttatacaa tgataggggc caatatcagc aagtttctga tggatatcgt
      421 ttctcttgtc gatacgaaaa aaacagctga tatcgttgat atttcaaatc agtcttccaa
      481 agaggggatc aggatcgtca tcgaactgcg aagagatgcg gatgttgaga atctgaaaaa
      541 cctgcttata aagaaaacaa agctggagga tacatttggt gtcaatatgc ttgccgttac
      601 ggatggccgg ccggagaccc tgaacctgaa aaatatcatt cgttaccatg ttaattttca
      661 gcgtgagatc gcaacaagaa aatatcggac gcttttgaat aaagaactgg atagaaaaga
      721 aattcaggaa gggcttatca aagcatgcga tgtgatcgat ctgatcattg aaattttacg
      781 tggttccaaa gatgttaaaa cggcaaaggc atgtctgatg gaaggaaaag cggaaggcat
      841 ccggtttaaa tccagaatct caaaaaaaat ggcggaacag ctttgtttta cggaacggca
      901 ggcatccgcg attttggaaa tgcgtctgta taagctgatc ggtcttgaga tcgaagcatt
      961 aatgaaagaa cataatgaaa ccttgaaaaa tattgagaca tataaagata ttttaacaaa
     1021 tccggcttcc atggatcatg tgatcatccg cgaactggat tcttataaga aagaattcgg
     1081 cagggagcgc cgtaccagga tcgaaaacgc agcggagatc atactggaag agaaaaaact
     1141 tgaagagatg gagatcatgt tcctgatgga tcggttcggt tatgcaaaaa cgatcgatat
     1201 gcagacctat gaacggaaca gggaggctgc ggatgcagaa aataagtatg tgttttcctg
     1261 tatgaatacc ggacgtattt gtatatttac aaacaccgga cagatgcata caataaaagt
     1321 attggatatt ccgtacggaa aatttcgtga taaaggaacg ccgatcgata atatcagcaa
     1381 ttacagcagt caggatgaaa ctttaatata tatttgttcc ctgtcggatc ttgttgttca
     1441 gaaactggtc ttcgcaacaa aaaaggctat gctcaagcat gtatatggta cggaatttga
     1501 cgtaacaaaa cgcacggtag ccgccacaaa gcttgccgag caggatgaag tccttgctgt
     1561 ctgtccggtc actgaccaga aaaatatcgt tctgcagaca aaaaacagta catttttgaa
     1621 attttctgtt gatgaaatac cgcttaagaa aaaaggggcc attggtgtca gagggatgaa
     1681 gctttccgaa gatgatgaac tggaagcagt ttattttctg acaaatgg
//
LOCUS       DAOSYE010000531         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59695_length_1727_cov_2.914474, whole genome shotgun sequence.
ACCESSION   DAOSYE010000531 DAOSYE010000000
VERSION     DAOSYE010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59695_length_1727_cov_2.914474"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1337
                     /locus_tag="PLU43_08890"
     CDS             15..>1337
                     /locus_tag="PLU43_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HOO28568.1"
                     /translation="MAQDHIFTPLEDVPDNIATGLVNFIGNGVGKTHQEIWEIMGEEN
                     IKTFGKNYPGFFRHENAYQFLKSMNDVHVIVMRRFKGAVPPILDMQPVSSNEAIFIYR
                     SKRGMGDYMAGLIKGVSSYFKENIQVDTLSKTPGEIQLKLTFEQEIRSIKKYRFNQLL
                     SLGFIKKTAIKTALLNTILIGISGFVLFADPVKAGILAALVFAVSLLSSFLLNRPAKL
                     VADELQSLAERKFSENKHIDSNDEYELLIKLIQNIKSVVQKDFIGFNSVVDEMNTFNG
                     SLSEIATTMQSASNDITEVLDEVAVAATTQAEDTEKVVTTLNDSIQNVTRISDDSQKN
                     KENIEDAVISIENSFHNVQNTASQINSVLGKFREIKDSSNELQNNATNITQIVSIVSG
                     IAGQINLLALNASIEAARAGESGRGFAVVAEEVRKLSEETNHAVKDINN"
     assembly_gap    1338..1347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1348..1727
                     /locus_tag="PLU43_08895"
     CDS             <1348..1727
                     /locus_tag="PLU43_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOO28569.1"
                     /translation="LTGFVLNLDEVVVGIDAQYSILETENASLTEAVNNSSNSNNNLK
                     GVSELLIKTSQDLKTEAEHISSLFDNIQNLAAIAEENSAATEEASSNVAIYVDQISEL
                     SKQISVFDSMIKLFQEELKKYII"
BASE COUNT          537 a          382 c          368 g          430 t
ORIGIN      
        1 taaaaaaata tgatctggca caagatcaca tttttactcc tctggaagat gtccccgaca
       61 atatagcaac cgggctcgtt aattttattg gaaacggcgt cggaaaaacc catcaggaaa
      121 tctgggagat catgggcgag gaaaatatca agaccttcgg caaaaattat cccggctttt
      181 tccgtcatga aaatgcctat cagtttttaa aatccatgaa cgatgtgcat gtgatcgtta
      241 tgcgcagatt taaaggggcc gttcccccca ttctggacat gcagcctgtc tcctctaatg
      301 aagcgatctt tatttatcgc tccaaacgcg ggatgggtga ttatatggcc gggctgatca
      361 aaggtgtctc atcctatttc aaagaaaaca tccaggtgga tacgctcagc aagacaccgg
      421 gtgagatcca attgaagctg acatttgaac aggagatccg aagcattaaa aaatatcgtt
      481 tcaatcagct tctgtctctt ggttttatca aaaaaaccgc tattaaaaca gccctgctga
      541 acacgatcct gatcggaatc agcggttttg tactgtttgc agatccggta aaagcaggca
      601 ttcttgccgc acttgtattt gccgtttcgc ttctctcttc ttttctgctg aacagacctg
      661 cgaaactggt tgctgacgaa ctgcaaagcc tggcagaacg taaattttcc gaaaataaac
      721 acattgattc caatgacgaa tacgaactgc tgatcaaact gattcaaaat attaagagcg
      781 ttgtgcaaaa ggacttcatc ggcttcaatt ccgttgttga cgaaatgaat acgtttaacg
      841 gttcgctttc cgaaattgcc actacaatgc aaagcgcctc caatgatatt acagaagtac
      901 tggacgaagt ggccgtagct gccacgacac aggctgaaga tacggaaaaa gtcgttacga
      961 ccttaaacga cagcattcag aacgtgaccc gtatttcaga cgacagtcag aaaaataaag
     1021 aaaatatcga agatgccgtg atcagtattg aaaacagctt ccataacgtg cagaatacgg
     1081 cttctcagat caactctgtt ttgggaaaat tcagggaaat taaagacagc agcaatgaac
     1141 tgcagaacaa tgctaccaat atcactcaga tcgtttccat cgtatccgga atcgccgggc
     1201 agatcaatct gcttgccctg aacgcttcca ttgaagcggc cagagccggg gaaagcggac
     1261 ggggcttcgc agtcgttgcg gaagaagtac gcaagctttc cgaagaaacg aatcatgcgg
     1321 taaaagatat caacaacnnn nnnnnnngcc tgaccggctt tgtattaaac ctcgatgagg
     1381 tcgttgtcgg tatcgatgcc cagtattcta ttctggaaac tgaaaatgca tcgctgactg
     1441 aagcagtcaa taattcaagt aattcaaata acaacctgaa aggagtttca gagcttctga
     1501 tcaaaacatc ccaggatctg aaaacagagg ccgaacatat ttcatcactg tttgacaaca
     1561 ttcagaatct ggcagcaatt gcggaagaaa attctgctgc gacagaagaa gccagctcca
     1621 atgttgcgat ctatgtcgat cagatcagtg agctttccaa acagatctct gtctttgatt
     1681 caatgatcaa gctgttccag gaagaactca aaaaatacat catatag
//
LOCUS       DAOSYE010000532         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_59790_length_1725_cov_3.168862, whole genome shotgun sequence.
ACCESSION   DAOSYE010000532 DAOSYE010000000
VERSION     DAOSYE010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59790_length_1725_cov_3.168862"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLU43_08900"
     CDS             complement(<1..514)
                     /locus_tag="PLU43_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO28570.1"
                     /translation="MEAVRSIGEFIQNQILGMKWLNTLIGTLLGKIGIDTAGKAGGSL
                     LFFLYDVIKITLLLCFLIFLISYIQSFFPPERSKKIMGRFHGIWANIIGALLGTVTPF
                     CSCSSIPLFIGFTSAGLPLGVTFSFLISSPMVDLASLLLLMSIFGWKVAVVYVAVGLV
                     VAVIGGTVIEK"
     gene            complement(605..925)
                     /locus_tag="PLU43_08905"
     CDS             complement(605..925)
                     /locus_tag="PLU43_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO28571.1"
                     /translation="MMGHSEDARMMKALADENRLSILELLQDGEKCACVLLEELNITQ
                     PTLSHHMKILCDSGIVEGRRDGKWTHYSLSAEGSGKLKERILYYTAGADTGGRNIKCD
                     ACKR"
     gene            complement(1020..>1725)
                     /locus_tag="PLU43_08910"
     CDS             complement(1020..>1725)
                     /locus_tag="PLU43_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28572.1"
                     /translation="ETTGDESQAREEEADEQNGGPAEIPWEAHIDYPAADVAIALNDQ
                     TAVYGEDAEDMMKNQGGSFGQISEKKAGDGYYEFLAYQGEPACMPMYAPLDSFYGMEQ
                     GGNQAILVKFQSNAVDPCFSLKAGKEYGIRFGADGVPAFWQEDTNSMEKLPSNVTLNE
                     GEWYYLLMAIDKNGTFRSILWPEQESEQKSYANRDLGDENQNGSWNFLLTFAENGSIK
                     IGYYVIFTYESLTPFD"
BASE COUNT          488 a          457 c          283 g          497 t
ORIGIN      
        1 gcttttcgat caccgtacca ccgatcaccg caacaacaag gccgaccgcc acataaacaa
       61 cggcaacttt ccagccaaaa atactcatca gcagtaaaag agacgcaaga tccaccatcg
      121 gtgacgaaat cagaaaggaa aacgtaacgc caagcggcag gcctgcactt gtaaagccaa
      181 tgaacagcgg aatcgacgag caggaacaaa aaggcgttac cgttccaaga agcgcaccaa
      241 ttatattggc ccatatgcca tgaaacctgc ccatgatctt tttacttctt tccggcggaa
      301 aaaagctctg gatatacgaa ataagaaaaa tcagaaagca taacagaagt gtgatcttta
      361 taacatcgta cagaaaaaat aaaagactgc ctcccgcctt tcctgccgta tcgataccga
      421 tcttgccaag caatgtcccg atcagtgtat tcagccattt cattccaaga atctgattct
      481 gaataaactc tccgattgat cttactgctt ccatcataaa tgtcctcctg tatttcattt
      541 atatatttat atgtatctat ttatttaata aatatgcggg taatccgaag attgcccaaa
      601 tattttatcg tttacatgca tcacacttta tattgcggcc gcctgtatcc gcaccggccg
      661 tataatataa aatcctctcc ttcagctttc cgcttccttc cgccgacagt gaatagtgtg
      721 tccatttacc atccctgcgg ccttccacaa tcccgctgtc acataaaatt ttcatatgat
      781 gcgacagggt cggctgcgta atattcaatt cttccagtag aacacaggca catttttcgc
      841 cgtcctgcaa taattccaat atggaaaggc gattttcatc agcaagcgcc ttcatcattc
      901 tcgcatcttc actatgaccc atcatcatta cctcaaaatg tacttacagg cttccatacc
      961 tgtactatat cactcatata gatatacgtc aatatattaa tatttacttt tataattttt
     1021 caatcaaacg gagtcagact ctcataagta aaaataacat aatatccgat tttaattgat
     1081 ccgttttccg caaaggtaag caaaaaattc catgaaccat tttgattctc atcaccgaga
     1141 tctctgtttg cataactttt ctgttcactt tcctgttcag gccagagaat gctgcgaaat
     1201 gttccgtttt tatcgatcgc catcagaaga taataccact ctccctcatt cagggtaaca
     1261 tttgacggaa gcttttccat actgtttgta tcctcctgcc agaatgcagg aactccgtct
     1321 gcgccgaacc gaattccata ttcttttcct gcttttaaag aaaagcacgg atcaacagca
     1381 tttgactgga acttgacgag aattgcctga ttacccccct gctccattcc gtaaaacgaa
     1441 tccagcggcg catacatcgg catacatgcc ggttctccct gatatgcaag aaactcataa
     1501 tatccgtctc cagccttttt ttccgatatt tgcccaaaac tgcctccttg atttttcatc
     1561 atgtcctcag cgtcttcacc gtaaaccgca gtctggtcat tcaatgcgat tgccacgtct
     1621 gcagcaggat aatcaatatg tgcttcccat gggatttccg caggtccgcc gttctgctca
     1681 tcagcttcct cttcccgtgc ctgactttca tctcccgtgg tttct
//
LOCUS       DAOSYE010000533         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_60210_length_1718_cov_2.349970, whole genome shotgun sequence.
ACCESSION   DAOSYE010000533 DAOSYE010000000
VERSION     DAOSYE010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60210_length_1718_cov_2.349970"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLU43_08915"
     CDS             <1..268
                     /locus_tag="PLU43_08915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334513.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO28573.1"
                     /translation="MQLCHVIDRHNIQIEIYERGAGYTLASGTSACAAAAAGCRMGLT
                     DAAVSVHMPGGVLEVLVNEDGSVSLTGGVSYIGEITLSPEFFEN"
     gene            469..>1718
                     /gene="argS"
                     /locus_tag="PLU43_08920"
     CDS             469..>1718
                     /gene="argS"
                     /locus_tag="PLU43_08920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO28574.1"
                     /translation="MKTLLAMISGKMEKAFEQAGYDKKYGRATLSNRPDLCEFQCNGA
                     LAAAKAYKKAPIVIAGEVEAILKELPEFSQVQAVAPGFLNLKLRASFLREYLSGMQED
                     DKFGLAGNESPCKIIIDYGGPNVAKPLHVGHLRSAIIGESVKRICRYMGNEVIGDIHL
                     GDWGLQMGLIITELKKRCPALPYFDENFKGEYPKEAPFTISELEEIYPTASARSKEDE
                     QYKEEAMNATFLLQNGNRGYRAIWQHIMAVSVADLKKNYANLNVSFEWWKGESDVQSY
                     IPDMVEKLKADGYAYESDKALVVDVMEETDTKEIPPCMILKSDGAALYTTTDLATLEE
                     RRREFDPAQIIYVVDKRQDMHFVQVFRCAKKTGIIEADVKLDFLGFGTMNGKDGKPFK
                     TREGGIMRLETLIADINEEMYRKIT"
BASE COUNT          502 a          315 c          485 g          416 t
ORIGIN      
        1 tatgcagctg tgccatgtga tcgacaggca taatatccag atcgaaattt atgaacgggg
       61 agcaggttat acgctggctt cagggacaag tgcctgcgca gctgcggcag cgggctgcag
      121 aatgggactg accgatgcgg ctgtgtcagt acatatgccg ggcggtgtgc tggaggtgct
      181 tgtcaatgag gatggctccg tatcattgac agggggcgtt tcctatattg gggagatcac
      241 tctttccccg gaattttttg aaaactgata aaaactgatt gaaaaataaa gaggcggtgg
      301 tcgaaagacc ccgcctctat ccttatgtct gaaactaaaa tcggtaaaaa ggctatattt
      361 tttgtttaaa ttatggtaaa tttattaatg cgggaaagtt tacctgaaat gaaagatacg
      421 gtgcttttct aaaaaatgtc agtcgatgaa taaggagtgc agcagattat gaaaacttta
      481 cttgccatga tttccggaaa aatggaaaag gcttttgaac aggcgggata tgacaaaaaa
      541 tacggcaggg caacactgtc caacagaccg gatctatgcg aatttcagtg taacggggcg
      601 cttgcagcgg caaaggctta taaaaaggcg ccgatcgtga tcgcaggaga ggttgaggca
      661 atcttaaaag agctgccgga attttcacaa gtacaggcag tcgcgccggg ctttttgaat
      721 ttaaagctga gagcttcttt tttacgggaa tatttatcag gcatgcagga ggatgataaa
      781 ttcggtcttg cagggaatga gagtccatgt aagatcatca ttgattacgg cggtcccaat
      841 gtggcaaagc cgcttcatgt cgggcatctg cgttccgcca ttatcggtga aagcgtaaag
      901 cggatctgcc gttatatggg gaatgaggtg atcggagata ttcatctggg agactggggg
      961 ctgcagatgg ggctgatcat tacagaactg aagaaacgct gtcccgccct tccttatttt
     1021 gatgagaatt tcaagggaga atatccgaaa gaagcaccgt ttacgatctc agagcttgag
     1081 gagatctatc cgacggcaag tgccaggtcc aaagaagatg agcagtataa ggaagaggca
     1141 atgaacgcaa cgttcctttt gcagaacgga aacagaggat atcgcgccat ttggcagcat
     1201 atcatggcag tctcggtggc ggatctgaaa aaaaattatg ccaatctgaa cgtttccttt
     1261 gagtggtgga aaggggagtc ggatgtgcag tcttacatcc cggatatggt agaaaaactg
     1321 aaggcggacg gctatgctta cgaaagcgac aaagcgcttg tcgtagatgt catggaagag
     1381 accgatacaa aggaaatacc gccgtgcatg atcttaaaat cggatggtgc ggcattgtat
     1441 acgacgactg atctggcgac actggaagag aggcgcaggg agtttgatcc ggctcagatc
     1501 atctatgtgg tcgataagcg tcaggatatg cattttgtac aggtattccg ctgtgcaaaa
     1561 aagaccggaa taatagaagc ggatgtgaag cttgattttc ttggtttcgg aacgatgaac
     1621 ggaaaagacg ggaagccttt taagacaagg gaaggcggta ttatgcgcct ggaaacgctt
     1681 attgcggaca ttaatgaaga aatgtacaga aagatcac
//
LOCUS       DAOSYE010000534         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_60674_length_1710_cov_2.866465, whole genome shotgun sequence.
ACCESSION   DAOSYE010000534 DAOSYE010000000
VERSION     DAOSYE010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60674_length_1710_cov_2.866465"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLU43_08925"
     CDS             <1..376
                     /locus_tag="PLU43_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO28575.1"
                     /translation="EIACECISSGEADKIISLKREELKRRAQLLKSKLSGYYFRYVPD
                     SMFAWLKLPDDFSSSEFEKAANDSGVNVVSSEKFSVGGIVPPNFIRISLSGADSIAEF
                     EEGLAVLLQILNHEIGMAGGVL"
     gene            410..1015
                     /locus_tag="PLU43_08930"
     CDS             410..1015
                     /locus_tag="PLU43_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOO28576.1"
                     /translation="MSKSEIKRKWMDQMFFALCEYTSVSDHERSRFYDKTNLLKIKKN
                     DHFIKAGDVPDKIAFIVQGIFRVYYTTLSGDENTLVFRDENKFLSAYSAFLESTESAY
                     SFQALEDSVLLYISMKDYVELMTEGGCCQAVTTKYSQLLFVEKEKREKEFLGCDALER
                     YTNFINQEPVLSGRVPQLYIASYLGIKPETLSRIRKKLKLI"
     gene            1079..>1710
                     /locus_tag="PLU43_08935"
     CDS             1079..>1710
                     /locus_tag="PLU43_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28577.1"
                     /translation="MKYKAEIFDRLQYLYEETKFNDHQLHCVIKFKNRLNREVLEKSI
                     CLLLETVPILSCVYVHHDGDSYWESSGGFAFSEICVIVNNEEDFHLFTASKILEEKGP
                     QIKFCFYRSENDSLSVIMNHMVCDAAGFKMLLYLFSDIYSKLTDDPAYMPKDIYHGDR
                     SIRKVIERFGVIDKVKSLLFQNGDNNKNDGYLFPMDQDDEIQPFILTHEI"
BASE COUNT          519 a          269 c          404 g          518 t
ORIGIN      
        1 ggagatcgca tgtgagtgta tttccagcgg ggaagcggat aaaatcattt cattaaaacg
       61 ggaggaactt aaacgcaggg cgcagcttct gaaatcaaaa ctgtcagggt attatttccg
      121 atacgtgccg gacagcatgt ttgcatggct caagctgccg gatgatttta gcagcagtga
      181 attcgaaaaa gccgcaaacg acagcggcgt caatgtggtt tcgtctgaaa agttttccgt
      241 aggcggtatt gtgcctccaa attttatacg tatttcttta agcggagcag acagtattgc
      301 agaatttgaa gaaggccttg ctgttctgct gcagattctg aatcatgaga tcggcatggc
      361 gggcggcgtt ttatagaaaa tgcagcgggg cttctttggg agataaaata tgagcaaatc
      421 agagatcaaa agaaaatgga tggatcagat gtttttcgca ttatgtgagt atacctctgt
      481 ttcggatcat gaacggagca ggttttatga taaaacaaat cttcttaaaa tcaaaaaaaa
      541 tgatcatttt ataaaagccg gggatgtgcc ggataaaatc gcctttattg tccaggggat
      601 atttcgtgtt tattacacga ctttgtcagg agatgaaaat acgcttgtat ttcgggatga
      661 aaataagttt ctgtctgcat acagtgcttt tttggaaagc actgaatcag cctattcgtt
      721 ccaggccttg gaggatagtg ttttgttata tatttcaatg aaggattatg tggagctgat
      781 gacagaaggc ggctgttgtc aggctgttac aacaaaatac tcacaattgc ttttcgtgga
      841 gaaggagaaa agagaaaaag aatttctggg atgtgatgca ctggagcggt atacaaattt
      901 tatcaatcag gagcctgttc tttctggccg ggtgccgcag ctttatattg cttcctatct
      961 tggaattaaa ccggagacat taagcaggat cagaaaaaag ttaaaattga tttagatcaa
     1021 tgcagttgct tttttgatcg tgtatacttg gtatatcaat aattgtgagg tatacggtat
     1081 gaaatacaaa gcagagatat ttgacaggct tcaatattta tatgaggaga caaaatttaa
     1141 cgatcatcag ctgcactgcg taataaaatt taaaaacaga ctcaacaggg aggtgctgga
     1201 aaaaagtatt tgccttttgc ttgaaacagt gccgattctg tcctgtgttt atgttcatca
     1261 tgacggtgat tcttattggg aatcttccgg cggttttgca ttcagtgaga tctgtgtaat
     1321 cgtcaataat gaagaagact ttcatttatt taccgcttca aaaattttgg aggaaaaagg
     1381 tccgcaaata aagttttgct tttatcgttc tgaaaatgat tcgctttcag tcataatgaa
     1441 ccatatggta tgtgatgctg caggatttaa aatgctgctg tatttgtttt cagacatcta
     1501 ttcaaaattg acagatgatc ccgcatatat gccaaaggat atttatcatg gggaccgcag
     1561 tatacggaaa gttatcgagc ggtttggggt cattgataaa gtaaaatcat tattgttcca
     1621 aaatggggat aacaacaaaa atgatgggta tctgtttccg atggatcagg atgatgagat
     1681 ccagccattc attttaacgc atgagataga
//
LOCUS       DAOSYE010000535         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61100_length_1703_cov_3.245752, whole genome shotgun sequence.
ACCESSION   DAOSYE010000535 DAOSYE010000000
VERSION     DAOSYE010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61100_length_1703_cov_3.245752"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1382)
                     /gene="eno"
                     /locus_tag="PLU43_08940"
     CDS             complement(87..1382)
                     /gene="eno"
                     /locus_tag="PLU43_08940"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO28578.1"
                     /translation="MDYLQIEKVIGREILDSRGNPTVEAEVLLADGTVGRGTAPSGAS
                     TGEFEALELRDGDKARYLGKGVTKAVANINTVINDTITGMDASDIYAIDQAMMAADGT
                     EDKSNLGANAILAVSIACARAASISLNISLYRFLGGISGNRLPVPMMNILNGGAHAAN
                     TVDVQEFMIMPVGAKSFKEALRWSAEVFHALASLLKEKGLATSVGDEGGFAPNLASDS
                     EAIEYILKAVEKAGYEPGRDFMLAVDAASSEWKGSEKGKYKLPKAGTEFTSAELIAYW
                     KELVEKYPIVSIEDALDEEDWEGWQLLTKELGDKVQLVGDDLFVTNTDRLMKGIRIGC
                     GNSILIKLNQIGSVSETLEAIKMAHKAGYTAVTSHRSGETEDTTIADLAVAMNTCQIK
                     TGAPSRTERVAKYNQLLRIEEELGDAAVYPGMGAFNIIK"
BASE COUNT          403 a          451 c          345 g          504 t
ORIGIN      
        1 tatttttgta tataaaatgc catatatagg aacctacata tggcatctta tttataaaca
       61 gtcatccctg aaaaaccagg gagctcttat tttatgatat tgaatgcacc cattcccgga
      121 tacacggcgg catcgccaag ctcctcttcg attcgaagca gctgattgta tttggccaca
      181 cgttcggtcc tgctcggcgc accggtcttt atctggcacg tattcattgc taccgcaaga
      241 tccgcgatcg tcgtatcttc tgtttctcct gaacggtgag atgtcactgc cgtataaccg
      301 gccttatgcg ccatcttgat cgcttcaagc gtttccgaca cggatccgat ctgattgagc
      361 ttgatcagga tcgaatttcc gcatccgatg cggatcccct tcataagccg gtccgtattt
      421 gtcacgaaca gatcatcccc tacaagctgt actttatcgc caagctcttt tgtaagcagc
      481 tgccagcctt cccagtcttc ttcgtccagt gcgtcttcaa tggaaacgat cggatatttt
      541 tcaacaagct ctttccagta tgcgatcaat tccgccgagg taaactctgt tccggccttc
      601 ggcagtttat atttaccctt ttcactgccc ttccattcac tggatgccgc gtctaccgcc
      661 agcatgaaat ccctgccagg ctcatagccg gctttttcaa ctgcttttaa aatatactcg
      721 atcgcttcgg aatcactggc aagattcggc gcgaagccgc cctcgtctcc aactgaggtc
      781 gcaaggcctt tttcttttaa taatgacgca agtgcatgaa atacttccgc tgaccagcga
      841 agtgcttcct taaagctttt cgcaccgaca ggcatgatca taaactcctg tacgtcgacc
      901 gtattggccg catgcgcacc tccgtttaaa atattcatca tcggaaccgg cagtctgttg
      961 ccggaaatac cgccaagaaa acggtataac gaaatattca gcgaaataga tgctgctctg
     1021 gcacatgcta tggaaacagc aaggatcgcg ttcgctccca aattggattt gtcttctgtt
     1081 ccgtctgctg ccatcattgc ctgatcgatc gcataaatat cggaagcgtc cattcctgta
     1141 atcgtatcgt tgatcaccgt attgatattg gcaaccgctt ttgtcacacc ttttccaaga
     1201 tatcttgctt tatcaccgtc tctgagctcc agcgcctcaa attcaccggt agaagctccg
     1261 ctcggtgcgg ttccgcgtcc tacggtccca tcggccagaa gaacttcggc ctctacggta
     1321 ggatttcccc gggagtccaa aatttccctg ccaattactt tttcgatctg taaataatcc
     1381 atttgtatag tcctcctttt gattcgaacg cactgcgcag acagacctcc cggaagggca
     1441 tatcccttcc ggcgaaaagc ggattcagca gctccgtttt ctatgaggcg tccacatgat
     1501 ttattctatt gcctgttggt tgtgtcatat atctaaatta tagtaaacac tcataaaaaa
     1561 tacaagactg cggcatgata aatttttgca aattttatcc ggcatctgct taattttgga
     1621 ggaacagatg ccggacagct atgtatgtat gttgtatgtg gtagttagtt atgtctaact
     1681 gcatttaata taccatggtt ttc
//
LOCUS       DAOSYE010000536         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61459_length_1697_cov_3.118149, whole genome shotgun sequence.
ACCESSION   DAOSYE010000536 DAOSYE010000000
VERSION     DAOSYE010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61459_length_1697_cov_3.118149"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLU43_08945"
     CDS             complement(<1..205)
                     /locus_tag="PLU43_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28579.1"
                     /translation="MKRMDVCNQKRAAGVLSLVLCFSILAGLTACGAGTGNDQDTSAA
                     ENMETVTEEETDVNAAILDTEEME"
     gene            complement(323..1507)
                     /locus_tag="PLU43_08950"
                     /pseudo
     CDS             complement(323..1507)
                     /locus_tag="PLU43_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          484 a          478 c          331 g          404 t
ORIGIN      
        1 tttccatttc ctccgtatcc agtatcgctg catttacatc cgtttcttcc tctgtaactg
       61 tttccatatt ttcagctgcc gaagtatcct gatcattacc tgtaccggca ccgcaggcag
      121 tcagtcctgc aagtatggaa aagcaaagca ccagtgacaa aacacctgct gctcgttttt
      181 gattacaaac atccattctt ttcataagtt tatccccctg tcgcatccga tcttgaatca
      241 ttttaaatac atatctgcca ttattataca tagtcctgaa atcagatgca acaatatttt
      301 cctatatgca gagaatgcct cttcacttcc ccatccgcag tctgataagc agcatggcga
      361 acggaaaaca gactgcagcc agcagacctg ctttgatccc atactctgaa aaaaagccgg
      421 ataccgtact gacgactgtt ggtcccgccg cacagccgat atcaccggca agtgccagaa
      481 acgcccacat tgccgtaccg ccctgcggac attcccccgc cgccatactg aatgttccag
      541 gccacataat accgaccgaa agaccgcaga tcgcacagcc tgcaagcgca agcatcggaa
      601 acggtgcaaa tactgtaagc agatagccga gtatacaaag caggcagctt ccaagcataa
      661 atttctgcag cgggatctta tggccatact tgccgtaata tacccttgaa actgccatca
      721 ttgcggcaaa cgcacatggt ccgaagagat ctccggtcgc ttttgaaact tttaagccgg
      781 attcagcaaa aaaggaagcc cattgtgaca ttgcctgttc ggaagcgcct gcacagatca
      841 tcagtattgc aaacagccag aacagcttca ttgaaagaag cgtcctgacc ggtatcgttt
      901 cctcttctgc cgacactgta cggatcggta caactgcaaa gaaaaaacaa ttcaaaaatg
      961 gtactgccgc ccagatgatc ggtacgatat accatcgtcc cataccaagt aatccgaacg
     1021 cggccgtaga cagcagcacg actcccatat gaccaaagca gtaaaacgaa tgcagcatgc
     1081 tcattgcttt cgccttttct ttagatggcg cagcttccac gatcggactg accagcacct
     1141 cgatgatccc gccgccgacg gcattgatga cgaccgcaca gacaagcccg gcatacggtt
     1201 ttgaaaataa aaacgggaaa acgcccattc caacaagacc gatcgctgcc attgcatgtg
     1261 caaatatgac agccgtccgg taaccgatcc ggtcaacata ttttgccgat aataagtcca
     1321 caatgatctg tgtaccgaaa ttgatcgtaa caagcagacc taccttttcc agggaaatat
     1381 caaagcttcg ctaaaatgtc aaaaacagca gcggacccag attatttatg atcgcctgca
     1441 caatataccc tgtatagcat gcatagatcg tctgcctgta actgatcctg ccacttaatt
     1501 tttccatata aaatcccgta cccggctgct ctgaaccgga tcctttctgc tgtgatatca
     1561 catatggcgg tcaaaatctg aagcgaccca gcgctcataa gcggcacgcc atgttttttc
     1621 cctgtacggc ctgaccggat ccccaatttc ttctgcataa gcttcaacga atttcagaaa
     1681 aaaggcaatc ttctcat
//
LOCUS       DAOSYE010000537         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61584_length_1695_cov_2.604878, whole genome shotgun sequence.
ACCESSION   DAOSYE010000537 DAOSYE010000000
VERSION     DAOSYE010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61584_length_1695_cov_2.604878"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLU43_08955"
     CDS             <1..204
                     /locus_tag="PLU43_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOO28580.1"
                     /translation="IQNAVLVPNETITDTPPKELPMDAVLLVYCRSGNRSKQASQKLA
                     DMGYMNVYEFGGINDWPYEVVTE"
     gene            233..964
                     /locus_tag="PLU43_08960"
     CDS             233..964
                     /locus_tag="PLU43_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO28581.1"
                     /translation="MRLLIVRHADPDYEKDSLTEKGWKEAQLLSKRLIKEEITEIYVS
                     PLGRARDTASLTLEKLGREAVVYDWLQEFPARINRPDVKDRKMIAWDWLPEDWMREPK
                     FFSAEHWSEQENMREGKAGELYREVTEGFDTLLKTQGYVRDGLYYRAVRPNRRTLLFF
                     CHFGLECVLLSHLLNVSPMIFWHQLCAAPSSVTTVYSEERREGKAIFRVSSFGDLSHL
                     YAAGETPSDSARFCETFDCPYERHD"
     gene            1085..>1695
                     /locus_tag="PLU43_08965"
     CDS             1085..>1695
                     /locus_tag="PLU43_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO28582.1"
                     /translation="MKKMIWVLAASLFVLTGCGSETTEAIQPAAEQTAEEQVGTQEDA
                     VEEEDTAQTTEETVQDEEAVDTEETGETEDAGEAEDTSSEMTGEFASFTSVDLDGNTV
                     DQSVIADADLTMLNIWGTYCGPCINEMPELGELADEYEGTGVQIIGVPIDVYDESTIE
                     TAKEIIDKTGADYLHILPTEDLYNILLNNVSAVPTTVFIDNTGT"
BASE COUNT          515 a          310 c          471 g          399 t
ORIGIN      
        1 attcaaaatg cggttcttgt accaaatgag acaattacag acacaccacc aaaagagctt
       61 cctatggatg cggttctgct tgtttattgc cgcagcggta accgcagcaa gcaggcatca
      121 caaaagcttg cagatatggg ttatatgaat gtgtatgaat ttggtggtat caatgattgg
      181 ccatatgagg tcgtaacgga ataacaataa aaaagaactg aaaagaggaa ttatgcgttt
      241 actaatcgta cgtcatgcgg atccggatta tgaaaaggat tccctgactg aaaaaggctg
      301 gaaagaggca cagctgctgt caaagaggct tatcaaggaa gagattacgg agatctatgt
      361 ttctccgctt ggaagggcaa gggatacggc atccctgaca cttgaaaagc tgggacgcga
      421 ggctgttgta tatgactggc tgcaggagtt tccggcaagg atcaaccggc cggatgtgaa
      481 ggaccggaaa atgattgcat gggattggct tccggaggac tggatgcgcg aacccaaatt
      541 tttttccgca gaacattggt ctgagcagga aaacatgcgg gaagggaagg ccggagaact
      601 ctaccgggaa gtgacggaag gctttgatac gctgctgaaa acacagggat atgtcaggga
      661 tgggctttat tacagagctg tacggcccaa tcgccggacg cttttatttt tctgtcactt
      721 tggattggaa tgtgtgctgt taagccatct gctaaacgtt tcaccgatga ttttctggca
      781 tcagctatgc gctgcaccca gttcggtcac gacagtctat tctgaagaga gaagagaggg
      841 aaaagccatt tttcgcgtaa gttctttcgg ggatctttca catttgtatg cagcaggaga
      901 gactccgtct gattcggccc ggttctgcga gacgtttgac tgtccatatg aaagacacga
      961 ttaaaaagat taaaaaaagg aatgattatt atttgcaaga taatagttga cagcacgaac
     1021 tgtaacaaat ataattacag tatgaaatta taaagtcgaa attatttaga aaaggagaaa
     1081 cattatgaag aaaatgatat gggttttggc tgcgtcatta tttgtactga ccggctgcgg
     1141 ctctgaaaca acagaagcga tacagccggc tgcggaacag acggcagagg aacaggtcgg
     1201 cacgcaggaa gatgctgtgg aagaggaaga tactgcacag acaacggaag agaccgtaca
     1261 ggatgaggaa gcggttgata cggaagagac aggagagaca gaggacgccg gtgaggcaga
     1321 agacacttcg tcagaaatga ccggtgagtt tgcaagtttt acctccgtag atctggatgg
     1381 aaatacagtt gatcagagcg tgatagcaga tgcggacctg acaatgctga atatctgggg
     1441 aacatactgc ggaccatgta tcaatgagat gccggagctg ggtgagctcg ccgacgagta
     1501 tgagggtacc ggtgtgcaga ttatcggagt gccgatagac gtttatgatg agagcacgat
     1561 tgaaacggca aaggagatca tcgataaaac aggagcagat tatcttcata tactgccgac
     1621 ggaagatttg tataatattt tgctgaataa tgtcagtgcg gttccgacta cggttttcat
     1681 tgataacaca ggaac
//
LOCUS       DAOSYE010000538         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61626_length_1694_cov_3.705918, whole genome shotgun sequence.
ACCESSION   DAOSYE010000538 DAOSYE010000000
VERSION     DAOSYE010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61626_length_1694_cov_3.705918"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLU43_08970"
     CDS             <1..261
                     /locus_tag="PLU43_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28583.1"
                     /translation="GSLENVVSVGSWFINSPVTKAITEKYGYTNPFEALESGSRDVVL
                     IDNCYPEEKGQYLSKHTGKEFEAELIGTENGYHSYRMKENVK"
     gene            258..1322
                     /locus_tag="PLU43_08975"
     CDS             258..1322
                     /locus_tag="PLU43_08975"
                     /inference="COORDINATES: protein motif:HMM:NF039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HOO28584.1"
                     /translation="MTGVVSIILIIIWGLAVPFILGLLIDRLLADSKTVLAARNFAFG
                     FMLMAAVFQLLAVPMILLHMRFHVLKNTWIAVILCLCVLSVILNFKTFGVKLKNRVSL
                     KKRPEITEVLIWAAAVAVIMIQTALLAGHMHVDTDDARFLAEAMEAVEQDTMLEYHPL
                     TGEFLGGPAGEMYKDITSPYPIFLGLLGELFKMPPAIAAHVVLPILLIPLCYAVYFVI
                     GSFFFDANMKYTGLFLLFLGLIHSFSFESIFAAGYTLLTIIWQGRSAAAMIMLPLLWY
                     VLLRMIREEKLKWIDYLLFAAVMLACAMLSGMGMLLSLLLAFAYVCAQAFLKRSVKPA
                     LLMLLCMWPNVIYLVLWKLR"
     gene            1339..>1694
                     /locus_tag="PLU43_08980"
     CDS             1339..>1694
                     /locus_tag="PLU43_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28585.1"
                     /translation="MKDFILDIIHMNNDYTGNGMYMALYFITLLFLAFYTREKKLKKE
                     VLLPSVLLLLGVYIAIPFVNNFIAQIYDDDIRARYFWILMMPAVTALGCTLFIRYLDT
                     KTKRIIAVLAFCPVIF"
BASE COUNT          417 a          312 c          435 g          530 t
ORIGIN      
        1 ggctctttag agaatgtcgt atctgtcggg agctggttca taaattcgcc tgttactaag
       61 gcgatcactg aaaaatacgg gtatacaaat ccgtttgaag cacttgagag cggcagcagg
      121 gatgtcgtac tgatcgataa ctgttacccg gaggaaaagg gacagtattt aagcaaacat
      181 acgggaaaag aattcgaggc agagctgatc ggaacagaaa acggatatca cagctataga
      241 atgaaggaga acgtgaaatg acaggtgttg tcagtatcat attgattatt atatggggac
      301 ttgccgtacc ttttatactt gggctgctga tcgacaggct gcttgccgat tcaaagacgg
      361 ttcttgccgc aagaaatttt gcatttggct ttatgctgat ggcagctgta tttcagctgc
      421 ttgccgtacc gatgatattg ctgcatatgc ggttccatgt gctgaaaaat acgtggatag
      481 cggtgatcct ttgtctgtgc gttctttctg tcatactgaa ttttaaaacg ttcggcgtaa
      541 agctgaaaaa cagggtttca ttgaaaaaac gtccggagat aacagaggtg cttatatggg
      601 ctgcagcggt tgccgtaata atgatccaga cagcgctgct ggcgggacat atgcatgtgg
      661 atacggatga cgcaagattt cttgccgagg caatggaagc agttgagcag gatacgatgc
      721 tggaatatca tccgcttacc ggtgaatttc tcggtggtcc ggcaggagaa atgtataagg
      781 atatcacatc tccgtatccg atctttttag gactgcttgg ggagctgttt aaaatgccgc
      841 cggcgatcgc agcgcatgtt gttttgccga tcctactgat tcctctgtgt tatgcggttt
      901 attttgtgat cggaagcttt ttctttgatg caaatatgaa gtatacggga ctgtttctgt
      961 tatttttagg cctgatccat tccttttctt ttgagtcgat ttttgcagca gggtatacgc
     1021 tgcttacgat catctggcag ggcaggtctg ctgctgcgat gatcatgctg cctcttttat
     1081 ggtacgtgct gcttcgcatg atcagagaag aaaagctgaa atggatcgat tatctgcttt
     1141 ttgcagctgt catgctggca tgtgccatgc tcagcggcat ggggatgctg ctttccctgc
     1201 tgcttgcgtt tgcttatgtt tgtgcacagg catttttgaa aagatcggta aaaccggctt
     1261 tgttaatgct tctttgtatg tggccgaatg ttatttattt ggtattatgg aagctgcggt
     1321 agtgctggag gaatcaatat gaaggacttt attttggaca tcatccatat gaataatgac
     1381 tatacgggaa acggcatgta tatggcgctg tattttatca cgcttttatt tcttgcgttt
     1441 tatacaaggg agaagaagct gaaaaaagaa gtgctgctgc cgtcggttct actgctgctc
     1501 ggtgtttata tagcaatccc gtttgtaaat aattttattg cacagatcta tgatgacgat
     1561 atcagggcaa gatatttctg gatactgatg atgcctgctg ttacagcgct tggctgtacg
     1621 ctttttatca gatatctgga tacgaaaaca aaacgtatca ttgccgtact ggcattttgt
     1681 ccggtcattt tttt
//
LOCUS       DAOSYE010000539         1693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61716_length_1693_cov_2.358974, whole genome shotgun sequence.
ACCESSION   DAOSYE010000539 DAOSYE010000000
VERSION     DAOSYE010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61716_length_1693_cov_2.358974"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /gene="fliD"
                     /locus_tag="PLU43_08985"
     CDS             complement(<1..1450)
                     /gene="fliD"
                     /locus_tag="PLU43_08985"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOO28586.1"
                     /translation="MSIRITGISSNLDTDSMVEELVSAYKEKGEDLEKDQTLLSWKQD
                     AWEDLSSEISTFYSKTLNNMRFSSTYTEKETTVSDETKATVVASDNAVNGTQTLEVTS
                     LAKAAYLTGGELSTTDDSTLSSSSTLADLGYTGDTTTITVNQGEQKEDGTYDTVTFDI
                     SSDTTISEFVSYMSAAGYNANFDSSSGRIFIGSQDSGAADNFDFDTASLDEDGTAALN
                     ALGLLDDGVKIDGQNATIILNGAEFESDTNTFTINGLTVTAKSLTEEDSPITLITDTD
                     YDSIYDSIRDFFSDYNSLINEMDALYNADSADDYEPLTDDEKDEMSDTEIEAWEEKIK
                     DAILRRDDTLSTVSSVLKDAMLQTYTVDGTTLSLSSFGISTLGYFDAADNEKNAYHID
                     GDEDDDDTSGNDDQLMSAIASDPEQVASFFQQLAQNLYTSLSKQMSTNEYRSYGSFYN
                     DKLMDEEYDDYTDKIEDWDDYVDDLEERYYQQF"
     gene            complement(1461..>1693)
                     /locus_tag="PLU43_08990"
     CDS             complement(1461..>1693)
                     /locus_tag="PLU43_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOO28587.1"
                     /translation="AEQQDLEKIRKAVDDLNKKMPHSEAQFGIHEATNRVTIKIIDKD
                     TKKVIKELPPEKTLDMIAKVWEMAGLMVDEKR"
BASE COUNT          420 a          426 c          298 g          549 t
ORIGIN      
        1 caaattgctg ataataacgc tcctccagat catctacata gtcatcccaa tcctctattt
       61 tgtctgtata atcatcatac tcttcatcca tcagcttatc gttataaaaa gaaccatagc
      121 ttctgtattc gtttgtgctc atctgtttag aaagtgaggt atacaggttt tgcgcaagct
      181 gctgaaaaaa gcttgccacc tgctccggat cagaggcaat tgcagacatc agctgatcat
      241 cattgccgga tgtatcgtca tcgtcttcat ccccgtcaat atgataggca ttcttttcat
      301 tatctgccgc atcaaaatat ccaagtgtac tgatcccaaa gctggaaaga ctcaatgtcg
      361 taccatctac cgtataagtc tgcagcattg cgtcttttag aacactggac acggtagata
      421 aggtgtcatc tctccttaat attgcatctt tgatcttttc ttcccatgct tcgatctccg
      481 tatcactcat ctcgtctttt tcatcatctg tcaaaggctc atagtcatct gcagaatctg
      541 cattataaag agcatccatt tcattgatca gactgttata gtcagagaaa aaatccctga
      601 tgctgtcata gatcgaatca taatccgtat ctgtaatcaa agtgattgga gaatcctcct
      661 cagtcagcga ttttgcagtc acggtcagtc cgtttatcgt aaatgtattt gtatcggatt
      721 caaattcggc accgtttaga atgatcgtgg cattctgtcc gtcgatcttc actccgtcat
      781 cgagcaggcc aagcgcattt agtgcagccg ttccatcttc atccaaagaa gccgtgtcaa
      841 aatcaaaatt gtccgcagcc ccggagtcct gactgccaat aaatatccgg cctgatgagg
      901 aatcaaaatt cgcattataa ccggcagcac tcatataact tacaaattca ctgatcgtcg
      961 tatccgaact tatatcaaac gtaaccgtat cgtatgtacc gtcctctttc tgctctccct
     1021 gattgactgt gatcgttgtc gtatcacccg tatatcctaa gtcagcgagc gtcgaagagg
     1081 aagacagcgt cgagtcatct gttgtggaaa gctctccgcc tgtcaggtac gctgcttttg
     1141 caaggcttgt cacttccagt gtctgtgtac cgtttaccgc attatcactt gcaacgaccg
     1201 tagcctttgt ttcatcggaa actgtcgttt ctttttccgt atatgtcgaa gaaaaacgca
     1261 tgttatttaa tgtttttgaa taaaaagtgc ttatttctga actcagatct tcccatgcat
     1321 cctgtttcca gctgagcagt gtctgatctt tttccagatc ttctcccttt tccttatacg
     1381 cgctgacaag ctcttcaacc atggaatccg tgtccagatt cgaactgata cctgttatcc
     1441 tgatagacat cctatacctc ctatctcttc tcgtcaacca taagacctgc catttcccat
     1501 acctttgcaa tcatatccag tgtcttttcc ggcggaagct ccttaataac cttttttgtg
     1561 tctttatcaa taattttaat tgttaccctg tttgtagcct catgaatacc aaactgtgcc
     1621 tccgaatgag gcattttctt gttcagatcg tccactgctt ttctgatctt ttcaagatcc
     1681 tgctgttctg ccg
//
LOCUS       DAOSYE010000540         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61850_length_1691_cov_2.706601, whole genome shotgun sequence.
ACCESSION   DAOSYE010000540 DAOSYE010000000
VERSION     DAOSYE010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61850_length_1691_cov_2.706601"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLU43_08995"
     CDS             complement(<1..665)
                     /locus_tag="PLU43_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO28588.1"
                     /translation="MKLDELKNIVRNSGIVGAGGAGFPTYMKLDERAETIIMNCAECE
                     PLLRLHRQLLRDKAQEILKIFAMLADTVHAKEAVIGIKESYQETIEAVEAFIGEYPGM
                     RIQKLPETYPMGDEVVLIYEATGKVIRPGGLPIEEGVAVFNVETVYNIYRAVEEQMPV
                     MSKYVTIVGEVAQPVTVRMPLYTGDAIQNQVRETVALQEKARNDLEAARRGVTQATRQ
                     LFLG"
     gene            complement(672..938)
                     /locus_tag="PLU43_09000"
     CDS             complement(672..938)
                     /locus_tag="PLU43_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOO28589.1"
                     /translation="MIVGKVTGSIVSTRKNENLIGNKFMIIEPITSMNGETHQFVAID
                     NIGAGVGEIVLVATGSAARIGCGMADAPIDAAIVGIVDEKNGLE"
     gene            complement(973..1617)
                     /locus_tag="PLU43_09005"
     CDS             complement(973..1617)
                     /locus_tag="PLU43_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOO28590.1"
                     /translation="METENITMITRLVMEALEKKEQTGNGFMVPIGVSARHVHLTQEH
                     VEILFGEGYRLTKKKELMGGQFASNEQVTIVGIKLRAIENVRILGPVRSASQVEISAT
                     DAVKLGIKAPIRESGNVAGSAPIALVGPKGALYLKEGCIVAMRHIHMAPADAQAAGVK
                     DGDIVSVKADNTRGTIFNHVKIRVKDSFSLEMHIDTDEANACSIKTGDTVTIVK"
BASE COUNT          343 a          504 c          345 g          499 t
ORIGIN      
        1 ccgagaaaga gctgccgcgt ggcctgggtc acgccgcggc gggcagcctc caggtcgttg
       61 cgcgccttct cctgcagcgc caccgtctcg cggacctggt tctggatcgc gtcgccggtg
      121 tagagcggca tcctgacggt gaccggctgt gcaacctcac cgacgatcgt cacatatttg
      181 ctcatgaccg gcatttgttc ttccacagcc ctgtaaatat tataaacagt ttcaacatta
      241 aataccgcaa cgccttcttc aattggaagt ccgcccggac ggatgacttt tcccgttgct
      301 tcatagatca gaacaacttc gtcacccatc ggatatgtct ccggaagctt ctgtatccgc
      361 atacccggat attctccgat aaacgcttcc actgcctcga tcgtttcctg atagctttcc
      421 ttgattccga tgaccgcctc ttttgcatga acagtatccg caagcattgc aaatatcttt
      481 aagatctcct gtgccttatc acgcagaagc tgcctgtgca gacgaagcag cggttcgcat
      541 tcggcacagt tcatgataat ggtctctgcc cgctcatcca gcttcatata agtaggaaag
      601 ccggcacctc cggctccgac tattccgctg tttctgacaa tattctttaa ttcatccagc
      661 ttcatgccgc tctactccaa tccgtttttc tcatctacga ttccgacgat cgctgcatcg
      721 atcggcgcat ccgccattcc gcatccgata cgtgctgcgc ttcctgtggc aacaaggacg
      781 atctctccga caccggcacc gatattatcg attgctacga actgatgggt ttcaccgttc
      841 atggatgtga tcggctcgat gatcataaat ttatttccga tcaggttctc gttttttctt
      901 gtcgatacga tgcttcccgt cacttttcca actatcattg ctgcctccgc ttctatgctc
      961 tacggcatcc gtttatttta ctattgttac cgtgtcgccc gttttgatcg agcaggcatt
     1021 tgcctcatcc gtatcgatat gcatctccag tgaaaaactg tcctttacac ggatcttcac
     1081 atgattaaat attgtccctc ttgtattgtc ggccttgacg gatacaatat cgccgtcctt
     1141 tacacctgct gcctgtgcat ccgccggcgc catatggata tgacgcattg caacgatgca
     1201 gccctccttc agataaaggg ctccctttgg acctacaagg gcgatcggtg cgctccccgc
     1261 aacatttccg gattccctga tcggtgcctt aataccgagc tttacggcat ccgttgcaga
     1321 gatctcgacc tgagatgcac ttctgaccgg tcccaaaatt cttacatttt ctattgctct
     1381 aagcttgatc ccgacgatcg tcacctgttc gttagaggca aactgtccgc ccatcagctc
     1441 ttttttcttt gttaatcgat aaccttcgcc aaataaaatc tcaacatgct cctgtgtcag
     1501 atgcacatgt ctggccgata cgccgatcgg caccataaat ccgtttccgg tctgttcctt
     1561 tttctcaagc gcttccataa caagccttgt gatcattgta atattttccg tttccaattt
     1621 ccgcacctgc ctctacgagt tcagttttcc tgctatgcgg tcctgttccg gatatgcctt
     1681 tttatccatg c
//
LOCUS       DAOSYE010000541         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61864_length_1691_cov_2.238386, whole genome shotgun sequence.
ACCESSION   DAOSYE010000541 DAOSYE010000000
VERSION     DAOSYE010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61864_length_1691_cov_2.238386"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="cheB"
                     /locus_tag="PLU43_09010"
     CDS             complement(<1..1073)
                     /gene="cheB"
                     /locus_tag="PLU43_09010"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOO28591.1"
                     /translation="MKKKILIIDDSALMRRVFYDIINSDDRFEVVDTAKDGAEGFKLI
                     VSRKYDAVVLDVYMPRMTGLEMLKEMQASNVHANVLMASTTTGEGEKETLTALELGAI
                     DFIKKPENVADARMDTFKNRFLQLLDIVANAGSNARTERLFEKKSADTKAPTFTARYD
                     VNFHTPQKSAEKQAASVKSTLRPGAAVAGRKIVAIASSTGGPKALQLVIPKLPKGIKA
                     PILLVQHMPKGFTRSLAERLNELSEVSVSEAKTGDPLMEGHVYVAPGGQHLKVEKRDL
                     GYYVNCTDEPSREGVKPCANYMFESLCDTTYDEVVCVVLTGMGSDGTAGIKELDKKKN
                     IHVIAQDEDSSTVYGMPKSIAVAG"
     gene            complement(1070..>1691)
                     /locus_tag="PLU43_09015"
     CDS             complement(1070..>1691)
                     /locus_tag="PLU43_09015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOO28592.1"
                     /translation="YDIVSEDQIKIAMPMEQGKIILLPVDGEYNICFYTTTGLYQCLA
                     RVIDRYKSDNIFVLDMELTTDIRKYQRREYYRLNCVLDMKSREITEKDVNGLSERVRF
                     IDTDITFNNGLIVDISGGGVRFISKVCYPKNTNILFSFSLYVSGKLAEYKLIGKVLLS
                     ERLENREDEYEHRIQFVNIMNDDRESIIRYIFEEERKIRRREKGKI"
BASE COUNT          420 a          457 c          289 g          525 t
ORIGIN      
        1 cctgccactg caatactttt cggcatacca tataccgtac tgctgtcttc atcctgcgca
       61 atgacatgaa tatttttctt tttatcaagt tccttaatac ctgccgtgcc gtcgctgccc
      121 ataccggtaa gcacgacaca gacgacctca tcgtaagttg tatcgcataa tgattcaaac
      181 atatagtttg cacacggttt tacaccttcc cttgaaggct catccgtgca gttgacataa
      241 tatccgaggt ctcttttctc gaccttcaaa tgctgtcctc ccggcgccac atatacatgc
      301 ccttccataa gcggatctcc ggtctttgct tccgaaacgg acacctcact taattcattc
      361 aatctttcag ccagtgaacg tgtaaaccct ttcggcatat gctgtacaag caggatcggt
      421 gctttaatac cctttggcag ctttggaata acaagctgca gagctttggg gcctcctgtg
      481 gaactggcaa ttgcaacgat cttgcgtccg gctaccgcag cacccggccg gagtgtcgat
      541 ttcaccgatg cagcctgttt ctctgccgat ttctgcggcg tatgaaaatt cacatcatac
      601 cgcgctgtaa aggtcggtgc tttcgtatca gcggattttt tttcaaaaag acgttccgtc
      661 cttgcattgc tgcctgcatt tgcaacgata tcaagcaatt gcagaaaacg atttttaaac
      721 gtatccattc tggcatctgc cacgttctca ggctttttaa taaaatcaat cgcaccaagc
      781 tccagtgcgg tcagggtttc cttctccccc tctcccgttg tcgtgctcgc catcagaaca
      841 ttcgcgtgga cattgcttgc ctgcatttcc ttaagcattt caagcccggt cattctcggc
      901 atataaacat caagaactac cgcatcatac ttcctggaaa caattaattt aaaaccttcc
      961 gcaccgtctt tcgccgtatc aacaacttca aatctgtcat ctgaattaat tatatcataa
     1021 aacactcttc tcataagtgc agaatcatca attattaata tttttttctt catatttttc
     1081 ccttttccct gcggcggatt tttcgctctt cttcaaaaat ataacggata atactttccc
     1141 tgtcatcatt catgatattc acaaactgga ttctgtgttc atactcgtct tccctgtttt
     1201 caagcctctc cgacagcagt accttgccga tcagcttata ttccgccagc tttccgctga
     1261 cataaagtga aaatgaaaac aatatgtttg tatttttagg ataacacact tttgaaataa
     1321 agcgtacccc ccctccgctg atatcaacga taagaccatt attgaatgtg atatccgtat
     1381 cgataaatct tacccgctcg ctgagaccgt ttacatcttt ttccgtaatc tcccggcttt
     1441 tcatatcaag cacacagttc agccggtaat attcgcggcg ctggtacttc ctgatatctg
     1501 ttgtcagctc catatccaga acaaaaatat tatcgctctt atatcggtcg atcacccttg
     1561 ccagacattg ataaagtcct gttgtcgtgt aaaagcagat attatattcc ccgtcgaccg
     1621 gaagcagaat gatcttcccc tgttccatcg gcatcgctat tttgatctga tcctccgata
     1681 caatatcata c
//
LOCUS       DAOSYE010000542         1689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61975_length_1689_cov_2.564259, whole genome shotgun sequence.
ACCESSION   DAOSYE010000542 DAOSYE010000000
VERSION     DAOSYE010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61975_length_1689_cov_2.564259"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..819)
                     /locus_tag="PLU43_09020"
     CDS             complement(25..819)
                     /locus_tag="PLU43_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOO28593.1"
                     /translation="MVAKRKEDPPKPAAAWMNTFADLMNLLLCFFVLLFSMSTVDAQK
                     FELVAASFSDSFSIFSGGGSSFGEGLLISNGVSQLNELDQYMNSMGKAAEESETDDMI
                     DAIDKKKMQESEELAELIEEMLEEEKLDKEVDIDFTSEYVSLTLNGSLLFDSGSADIK
                     EESFDILDKIGVILQKYASSIIEIEGHTDNVPIHNAQFKSNNELSSARALSVFEYLVD
                     NTNLDPAMVKHSGRGEYVPIADNSTPEGRAKNRRVEIKIYHTLSTY"
     gene            complement(816..1613)
                     /locus_tag="PLU43_09025"
     CDS             complement(816..1613)
                     /locus_tag="PLU43_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOO28594.1"
                     /translation="MDLATIIGIVACLVFIVISISLGQDGIAGVQYFVDPPSAMITFG
                     GAFCCILASYKLQEFTKGIKSITIAFKLPVLNAGAIIQKIIDLSNVARKEGLLSLEEA
                     AGELDDSFLQKGIMLVVDGTDPDLVRGIMETELVSIDDRHKKNIGFWENLASMGPAWG
                     MIGTLVGLILMLYHMDDASTIGPSMAVALITTMYGSLLANWICTPIATKLKSINNEEM
                     QVKEIIIEGLLSIQAGENPRVIEEKLKSFMAPSERSSVGKDAGGGEE"
BASE COUNT          466 a          457 c          294 g          472 t
ORIGIN      
        1 tgctcctttg cggtaaaaca agctctaata agtactcagt gtatgataaa tcttaatttc
       61 cacacgcctg ttcttggccc gtccttccgg tgtggaatta tccgcgatcg ggacatattc
      121 gcccctgccc gaatgcttga ccatggccgg atccagattg gtattgtcaa cgagatattc
      181 aaaaacagac aaggcacgtg cactggaaag ttcattattg ctcttaaact gagcattatg
      241 aatcggaaca ttatcagtgt gcccctctat ttcaataata ctgcttgcat acttctgcag
      301 aatgactccg atcttgtcaa gaatatcaaa agactcctcc ttgatatcgg cactgcctga
      361 gtcaaacaaa agtgaaccat tcagcgtcag cgacacatac tcggacgtaa aatcgatatc
      421 cacttcctta tccagttttt cttcctccaa catttcttcg atcaattccg caagctcttc
      481 cgattcctgc atcttctttt tatcaatggc atcgatcata tcatctgtct cggactcttc
      541 tgctgccttg cccattgaat tcatatactg atccagttca tttaactggc tgactccgtt
      601 cgatatcagc agcccttccc caaatgatga accgcctccg gaaaaaatac tgaaactgtc
      661 cgaaaaggaa gcggctacca attcaaattt ctgcgcatct accgtcgaca tggaaaacag
      721 taatacaaaa aaacacaaca gcagattcat cagatctgca aatgtattca tccacgctgc
      781 agccggcttt ggcggatctt cttttcgttt tgccactatt cctcacctcc gcctgcatcc
      841 ttgccaacgc tgctgcgttc gctcggtgcc ataaaggatt tcagtttttc ctcaattaca
      901 cgcggatttt cccctgcctg aatagacagc agtccttcaa taataatctc ctttacctgc
      961 atctcttcat tattgatcga tttcagcttt gtcgcaattg gcgtacagat ccagtttgca
     1021 agaagcgagc catacattgt cgtaataagc gcaacagcca tggaaggacc gatcgtagac
     1081 gcatcgtcca tatgataaag catcagaata agacctacca gcgtaccgat cataccccaa
     1141 gcaggtccca tggatgcaag attttcccag aatccaatgt ttttcttatg acggtcgtcg
     1201 atactgacaa gctccgtctc catgattccc ctgacaagat ccggatccgt accgtcgaca
     1261 accagcatga tgcccttctg taaaaaagag tcgtcaagct cgccggccgc ttcttccagc
     1321 gacagcagac cttcttttct ggccacattc gataggtcga tgatcttttg aatgatcgct
     1381 cctgcattta aaacaggaag cttaaacgcg atcgtaatac tcttgatacc tttcgtaaat
     1441 tcctgcagct tgtaacttgc gagaatacaa caaaacgcac cgccgaacgt gatcatcgcc
     1501 gaaggtggat ccacaaaata ctgaacgcct gcaatcccat cttgtccaag tgaaatcgaa
     1561 ataacgataa acacaagaca ggccactata ccaattattg ttgctaaatc caatactccc
     1621 acctacctaa ttcacataaa tatccctttt ataagatttt actaaatttt tcacttcttg
     1681 tctacgttc
//
LOCUS       DAOSYE010000543         1689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_61976_length_1689_cov_2.559976, whole genome shotgun sequence.
ACCESSION   DAOSYE010000543 DAOSYE010000000
VERSION     DAOSYE010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61976_length_1689_cov_2.559976"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="modB"
                     /locus_tag="PLU43_09030"
     CDS             complement(<1..545)
                     /gene="modB"
                     /locus_tag="PLU43_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOO28595.1"
                     /translation="MELSPILISMKTALCGIVITFFLGILAARWVVYMKSRNWKMILD
                     GLFTLPLVLPPTVAGFFLLYIFGVKRPVGKFLVDFFAIKVPFSWGATVIAAVAIAFPL
                     MYRSARGAFEQVDENLLYAGRTLGMSEGTIFFRILLPNAMPGVLSGGILAFARGLGEF
                     GATTMIAGNIAGQTRTLPLAV"
     gene            complement(547..1545)
                     /locus_tag="PLU43_09035"
     CDS             complement(547..1545)
                     /locus_tag="PLU43_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOO28596.1"
                     /translation="MRTITKQQLEQMDIRQDRLVICGAGHVSLAVIRLGILLGFFVTV
                     IEDRPYFADQARRILEPEEAEGTKKGEVICEAFETALAGEKGSGNTYFVVVTRGHRYD
                     LDCLKSILKKPYAYVGMMASRGRALRVRTLLLEDGFLQEMIDTLHSPIGLKINAKTPE
                     EIAVSIMAEIIQVRGAQLQTSLILYQKGEIIVPGEEAGTIKEENDSDIKIDLIPEIKE
                     AALSDSDMRRVLTVITARRGSAPRTIGTAMLVFADGRIAGTIGGGCLEAEVIREALTR
                     LRLPEPAVLSDGQGQLPDRIEVQMLPEEAENEGMACGGILQVEFIEIKAAAERNFE"
     gene            complement(1575..>1689)
                     /locus_tag="PLU43_09040"
     CDS             complement(1575..>1689)
                     /locus_tag="PLU43_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO28597.1"
                     /translation="ADEKVTAQMLAAFGEGGLCLNCRQCTFPNCGFGKRSY"
BASE COUNT          429 a          514 c          319 g          427 t
ORIGIN      
        1 taaaccgcca gcggaagcgt acgcgtctgt cctgcaatat tgccggcgat catcgtcgtc
       61 gcaccaaact caccaagtcc tcttgcaaac gcaaggatcc ccccgctcaa aacaccgggc
      121 attgcattcg gcagcagaat tctgaaaaaa atggttcctt cagacatgcc aagcgtcctg
      181 cccgcataca gcagattttc atccacctgt tcaaaagcgc ctctcgcaga acggtacatc
      241 agcggaaacg caatcgctac tgcggcgatt accgtcgctc cccacgaaaa cggaaccttg
      301 atcgcaaaaa aatccaccag aaatttccca accggtcttt tgacgccaaa aatataaagc
      361 agaaaaaaac ccgcaacagt cggcggcaat acaagcggca gtgtaaataa tccgtccagt
      421 atcatcttcc agtttctgct cttcatataa acgacccatc ttgcggcaag aattcccaaa
      481 aaaaacgtaa taacgattcc gcaaagggca gttttcattg aaatcaagat cggggataat
      541 tccatactac tcaaaattcc tttccgcagc tgcctttatt tctataaatt ctacctgtaa
      601 aatgcctccg caggccatcc cttcgttttc cgcttcctcg ggaagcatct gcacttcaat
      661 tctgtccgga agctgtccct gtccgtcgga taaaacagca ggctccggca gacgaagacg
      721 cgtcagagcc tcacggatga cttctgcctc cagacagccg cctccgatcg ttcctgcgat
      781 ccgtccgtcc gcaaagacga gcattgctgt cccaatcgtc cttggtgcag aacctcttct
      841 ggcagtaatg accgtcagta cacgccgcat gtcactgtct gataaagcag cctctttgat
      901 ttccggaatc agatcgattt ttatatccga atcgttttct tccttgatcg ttccggcctc
      961 ttctcccggc acaatgatct ctcccttctg atataaaatc agagaggtct gcaattgcgc
     1021 tccccgcacc tgaatgattt ccgccatgat cgaaacggcg atctcctcag gcgtttttgc
     1081 attgatctta agtcctatcg gactatgcag tgtatcgatc atctcctgta aaaaaccgtc
     1141 ttccagcaaa agtgtcctga cacgcaatgc cctgcccctg cttgccatca ttccgacata
     1201 cgcgtaaggc ttttttaata tgctctttaa gcaatccaaa tcataacggt gccctcttgt
     1261 cacaacaaca aaataggtgt tgccggaacc tttttctcct gcaagcgctg tttcaaacgc
     1321 ttcacagatc acctcaccct ttttggttcc ctctgcctct tccggctcca gtatacgcct
     1381 tgcctgatct gcaaaatacg gtctgtcctc aatgaccgtc acaaaaaaac caagcagtat
     1441 tccaagcctg atcacagcca gcgatacatg acctgctccg cagatcacaa ggcgatcctg
     1501 cctgatatcc atctgttcca gctgctgttt tgtaattgtc cgcaccgtag tctctccttt
     1561 cccgctttta cggatcaata cgaccgcttt ccaaacccgc agttaggaaa ggtacattgc
     1621 ctgcagttta aacacaatcc gccttcccca aaggctgcaa gcatttgggc cgtaactttt
     1681 tcatccgcc
//
LOCUS       DAOSYE010000544         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62020_length_1688_cov_3.415799, whole genome shotgun sequence.
ACCESSION   DAOSYE010000544 DAOSYE010000000
VERSION     DAOSYE010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62020_length_1688_cov_3.415799"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLU43_09045"
     CDS             <1..961
                     /locus_tag="PLU43_09045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO28598.1"
                     /translation="EPNPKVTSVDEGVKICREHDIEVVLPVGGGSSIDCAKCISAAYY
                     YQGPAWDLVLNNSLAVKALPIITIITIAATGSEMDYFSVISNTETNDKLDFTSRLLYP
                     KYTIMDPEYTYTVPPYQTASGTADIMSHVFEIYFSSTENTYMQDRFMEGLLNTCVTYG
                     PIACREPANYDARANLLWTSSWAINGFISCGKGGPWPCHSIEHQLSAFYDVTHGHGLA
                     VIIPVWMNYILNDTTVELFVNYGTHVFGIDASKPKFEIAKEAIAKTKQLFIDMGLSLT
                     LRELGIPDKENFGIMAQKASAGLEHCLVPLNKDDIITILDQCF"
     gene            987..1325
                     /locus_tag="PLU43_09050"
     CDS             987..1325
                     /locus_tag="PLU43_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO28599.1"
                     /translation="MKKLEIFTRPEKLEGLKELLTVHKCQGMSVFTVMGCGKQKGYVP
                     EMNFTEFAINLLPKICIISIIDDDELEELLNDIYSVMGNGNIGDGKVFVYDVYEAMRI
                     RTGERGSNAL"
     gene            1343..>1688
                     /locus_tag="PLU43_09055"
     CDS             1343..>1688
                     /locus_tag="PLU43_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28600.1"
                     /translation="MGESIISLVNVVKAFGDNVVVNNLNLNVNEGEFLTFLGPSGCGK
                     TTTLRMIAGFETASEGIIKIEGERIEDKEPYERNVNTVFQNYALFPHMNVYDNIAYGL
                     ALKKVPKAEIKER"
BASE COUNT          500 a          368 c          380 g          440 t
ORIGIN      
        1 ggagccgaat cccaaagtca cttcagtgga tgaaggcgta aaaatctgcc gggagcatga
       61 tatcgaagtc gttctccccg ttggcggcgg cagttccatc gactgcgcaa agtgtatttc
      121 tgccgcttac tattatcagg gccctgcatg ggatctcgtt ctaaataaca gccttgccgt
      181 aaaagccctc ccgatcatca cgatcatcac gattgccgcc accggatctg aaatggacta
      241 tttctccgtt atttccaaca ccgaaacaaa tgataaatta gattttacaa gcagactgct
      301 ttatccgaaa tatacgatca tggatccgga atatacgtat accgtaccgc cctatcaaac
      361 agcatccggc accgccgata ttatgtccca tgtctttgaa atttatttca gcagtacgga
      421 aaacacctac atgcaggacc gttttatgga aggactgctg aatacctgcg tgacatatgg
      481 tccgatcgcc tgcagggagc ctgcaaatta cgacgcacgg gctaatctgc tgtggacttc
      541 cagctgggca atcaacggtt ttatctcctg cggcaaagga ggaccatggc cctgtcattc
      601 cattgaacac cagttaagtg cattttatga tgttacgcac ggacatggtc ttgccgtgat
      661 cattccagta tggatgaact atattttgaa tgatacgacc gtggagttgt ttgtaaatta
      721 cggcactcac gtattcggta tcgatgcttc caaacccaaa tttgagatcg caaaagaagc
      781 gatcgcaaaa accaaacagc tttttattga tatgggctta tccttaacgc tgcgagaact
      841 tgggatcccg gataaagaaa atttcggcat catggctcaa aaagcttctg ccggactgga
      901 acactgcctt gttccgctta acaaagacga tatcataacg attctggatc aatgtttcta
      961 aactattttg aatggaggta atgacaatga aaaaactgga aatttttacc cggcccgaaa
     1021 aactggaagg cctgaaagaa ttgctgaccg tacacaaatg tcagggaatg tccgtattta
     1081 ccgttatggg atgcggaaaa caaaaaggtt atgtcccgga aatgaatttt accgaatttg
     1141 cgatcaatct tcttccgaaa atatgtatca tttccatcat agatgacgac gaactggaag
     1201 agcttctgaa cgacatctat tctgtcatgg gaaacggaaa cataggagac ggcaaagttt
     1261 ttgtttatga tgtatatgag gcaatgagaa ttcgtaccgg cgaacgggga agcaacgctt
     1321 tatagggaag gagctgttta tcatgggaga atctattata tctttagtca acgtcgtaaa
     1381 ggccttcggc gataatgtgg ttgtgaacaa cttgaattta aatgtgaatg aaggagaatt
     1441 tctgaccttt ctcggacctt ccggctgcgg caaaactacc acgctgcgta tgatcgccgg
     1501 atttgaaacc gcttccgaag gaatcatcaa gatcgaagga gagcgtatcg aagacaaaga
     1561 gccttatgag cgcaatgtaa acactgtttt tcaaaattac gccctattcc ctcatatgaa
     1621 tgtttatgat aatatcgctt acggattggc cctgaaaaaa gtcccgaaag cagagatcaa
     1681 agaacggg
//
LOCUS       DAOSYE010000545         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62034_length_1688_cov_2.923454, whole genome shotgun sequence.
ACCESSION   DAOSYE010000545 DAOSYE010000000
VERSION     DAOSYE010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62034_length_1688_cov_2.923454"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLU43_09060"
     CDS             <1..218
                     /locus_tag="PLU43_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28601.1"
                     /translation="MKLIDSIRAAWGITYPFERNSFLQDPEPEPAEESRSSVRKVSEV
                     NSLSKGEDAERETEAKQEPKQEPETEA"
     gene            complement(270..533)
                     /gene="rpsT"
                     /locus_tag="PLU43_09065"
     CDS             complement(270..533)
                     /gene="rpsT"
                     /locus_tag="PLU43_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOO28602.1"
                     /translation="MANIKSAEKRILVIKTKTERNKAIKSKVKTYVKKVYAAIEANDK
                     ETANAALLEAISEISKATSKGVYHKSTSSRKISRMTKAVNAIA"
     gene            779..>1688
                     /locus_tag="PLU43_09070"
     CDS             779..>1688
                     /locus_tag="PLU43_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO28603.1"
                     /translation="MDQSRIRNFCIIAHIDHGKSTLADRIIERTGLLTSREMQAQVLD
                     NMELERERGITIKSQAVRTIYKAVDGTEYIFNLIDTPGHVDFNYEVSRSLAACDGAIL
                     VVDAAQGIEAQTLANVYLALDHNLDVFPVINKIDLPSAEPDRVVEEIEDVIGIEAQDA
                     PRISAKNGIGIEEVLEQVITKIPAPKGDPDAPLKALIFDSIYDSYKGVIVFCRLREGR
                     VRKGTLIRMMATGAAAEVVEVGYFGAGQLIPCEELSAGMVGYLTAGIKNVRDTTVGDT
                     VTNAVHPCDKPLPGYKKVQSMVFCGMY"
BASE COUNT          479 a          312 c          442 g          455 t
ORIGIN      
        1 tgatgaagct gatagacagc attcgtgcag catgggggat tacatacccg tttgaaagga
       61 attcttttct gcaggatcct gaacctgagc cggcagagga aagcaggagt tctgtcagaa
      121 aagtatctga ggtaaattct ttatcaaaag gtgaggatgc agaaagagaa acggaagcga
      181 aacaagagcc gaaacaagag ccggaaacgg aagcataaaa aaagaacggt ccacatgacg
      241 gtatggactg ttctttttat gaatgtattt taagcaattg cattgactgc ttttgtcata
      301 cgggaaattt ttctggaaga agtactttta tgatatacac cctttgacgt agctttgctg
      361 atttccgaaa tagcttcgag caatgcagca ttagcagttt ctttatcgtt tgcttcaata
      421 gcagcgtata ctttcttaac gtaagtcttt acttttgact tgatcgcttt attgcgttcc
      481 gtttttgttt tgataacaag aattcttttt tctgctgact taatatttgc cacgatttgc
      541 acctcctgtt tccatagagt aaggctgcgg caatgccgct cctccaatgt catattgaat
      601 ccggacacgg acgcctcaat atgagcacac taatatagtt tagtagaagt aaatacttct
      661 gtcaatactt tttttacaaa aagcaggaat attgatttgc ttgcaatatt tttatgctgc
      721 atttatacta ttacaagaat gaaatggaaa gaatacatac gatgcgggag aattgataat
      781 ggatcagagc agaatcagga atttttgtat tatagcgcat attgaccatg gaaaatcgac
      841 acttgcggac aggatcatcg agcggacagg actgcttaca agccgggaga tgcaggcgca
      901 ggtgcttgat aatatggaac tggagagaga gcggggaatt acgatcaaat cgcaggccgt
      961 acgtacgatc tacaaagcag tggatggtac ggaatatata ttcaatctga tcgatacccc
     1021 ggggcatgtt gattttaatt atgaggtcag caggagcctt gcggcatgtg acggagcgat
     1081 ccttgttgtc gatgcggcac agggaattga agcgcagacg cttgccaatg tgtatctggc
     1141 actggaccat aaccttgacg tatttccggt cattaataaa attgacctgc cgagtgcgga
     1201 accggatcgt gtagtggagg agatcgagga tgtgatcggg atcgaggcgc aggatgcgcc
     1261 gcggatttct gcgaaaaacg gaatcggtat cgaagaagtt ttggaacagg tcattacaaa
     1321 aataccggcg ccaaaaggag atccggatgc gccgcttaag gctttgattt ttgattccat
     1381 ctatgattct tacaaaggtg tcatcgtgtt ctgccgctta agggaaggca gggtcagaaa
     1441 gggcacattg atcaggatga tggcgacagg cgcagcggct gaggtcgtgg aagtgggata
     1501 ttttggagca ggacagctga ttccatgcga ggagctttca gccggtatgg taggctatct
     1561 gactgccggt attaaaaatg tcagggatac gactgtcggc gatacggtga caaatgctgt
     1621 acatccatgc gacaagccgc tgccgggtta taaaaaggtg cagtcgatgg tattctgcgg
     1681 tatgtatc
//
LOCUS       DAOSYE010000546         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62088_length_1687_cov_3.284314, whole genome shotgun sequence.
ACCESSION   DAOSYE010000546 DAOSYE010000000
VERSION     DAOSYE010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62088_length_1687_cov_3.284314"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..998
                     /locus_tag="PLU43_09075"
     CDS             81..998
                     /locus_tag="PLU43_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO28604.1"
                     /translation="MRRFKTKAKEFLPFYLMALPGTIYLICNNYMPMYGILLAFKKLN
                     VQKGIFGSAWSGFDNFKFLFQTNTAFLIIRNTILYNLSFIIFGTFIAMVLAVCLNEIK
                     KKTASRLYQSLILIPYLMSWVIASYLVYALLSQDVGLLNKGILEPLGLEAVNWYGTKK
                     YWPAILFVCNTWKTSGYMMIIYYSSIVSISSDYYEAAMIDGGTKWQQIKYITLPLIKS
                     TVIVMVILSIGRIASSDFGLFYQILRNSGSLYSVTQTIDVYVYNALMKNNDFGMSSAA
                     SVFQSIVGFAFIMTANGIVKRVHRESALF"
     gene            1010..>1687
                     /locus_tag="PLU43_09080"
     CDS             1010..>1687
                     /locus_tag="PLU43_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28605.1"
                     /translation="MRSHDELKWKIISNGVLLVFTLAAVLPFLLLLMASVSKESVVMT
                     NGYSFFPQEFSPDAYNYILSQADTLGRGYLNTIIVTMLGTGVSMLLTSMFAYALSNDR
                     LPGVKLLNFLCIFTMLFSGGQVASYYVWSSMFKVRDTVWGLVLPNLLMNAFNVILVKN
                     YFINSIHVSLKEAARIDGCGEFKIFRKIVMPLSIPINATIGLMTGLIYWNDWVNGLYY
                     LTARNGSR"
BASE COUNT          456 a          317 c          412 g          502 t
ORIGIN      
        1 atgctgtcct gagcaggcat ctccttgtat tttcacaaat gagcactgcg gtgatgggaa
       61 gagaggttat aaattgagaa atgagaagat ttaaaacaaa ggcaaaggag tttttaccgt
      121 tctatctgat ggctctgccc ggtactattt atttgatctg caacaattac atgccgatgt
      181 atgggatcct gctggcattt aaaaagctga atgtacaaaa ggggattttt ggaagtgcat
      241 ggagcggatt cgataatttc aagtttctgt ttcagacaaa cacggcattt ttgatcatcc
      301 ggaatacgat tttgtacaat ctgtccttta ttattttcgg aacattcatt gccatggtac
      361 ttgccgtctg tttgaatgaa ataaagaaaa agacagcgtc cagactgtat cagagtctga
      421 tcctgattcc atatctgatg agctgggtca tagccagcta tcttgtatat gccctgcttt
      481 cgcaggatgt cggtctgctc aacaaaggga ttttggaacc gctcggactg gaagccgtca
      541 actggtacgg aacaaaaaag tactggccgg ccatactgtt tgtatgtaat acatggaaaa
      601 catcggggta tatgatgatc atttattatt cgagtatcgt cagtatcagc agcgattatt
      661 atgaggcggc aatgatagac ggcggtacga aatggcagca gatcaaatat attacgctgc
      721 cgctgatcaa atcgactgtc atcgtcatgg tgatcttgtc gatcggaaga attgcttctt
      781 ccgatttcgg attattttat cagatactgc gcaattcggg ttctctttat tctgtcacac
      841 aaacaataga tgtctatgta tacaatgcgt tgatgaaaaa caatgatttt ggaatgtctt
      901 ccgctgcaag cgtttttcag tcgattgtcg gttttgcgtt tattatgacg gcaaacggca
      961 ttgtaaaaag agtccatagg gaaagcgctt tattttagga ggagacggca tgaggagtca
     1021 tgatgaatta aaatggaaaa ttatctccaa cggggtactg cttgtattta cacttgcggc
     1081 cgtactgcct tttctgctgc tgctgatggc ttccgtcagt aaggaaagtg tggtcatgac
     1141 aaacggctac agtttttttc cgcaggaatt cagtccggat gcatataact acatattatc
     1201 gcaggcagat acacttggaa gaggatattt gaatacgatc atagtgacaa tgcttggcac
     1261 cggcgtcagc atgctgctta caagcatgtt tgcctatgcc ctgtcaaatg accggcttcc
     1321 gggtgttaag ctcctgaatt ttttatgtat ttttacaatg ctgttcagcg gcggacaggt
     1381 ggcaagctat tatgtatgga gcagtatgtt taaagtaagg gatacggtct ggggactggt
     1441 gctgccgaat cttctgatga atgcgttcaa tgtcattctg gtcaagaatt attttataaa
     1501 cagtattcat gtttccctga aagaagctgc gcgtatagac ggctgcgggg agtttaaaat
     1561 ctttcggaag atcgtaatgc cgctgtcgat cccaatcaat gcgacgattg gtctgatgac
     1621 cggtctgatc tactggaacg actgggtaaa cggactgtat tacctgacgg cccggaacgg
     1681 aagcaga
//
LOCUS       DAOSYE010000547         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62094_length_1687_cov_3.098652, whole genome shotgun sequence.
ACCESSION   DAOSYE010000547 DAOSYE010000000
VERSION     DAOSYE010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62094_length_1687_cov_3.098652"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLU43_09085"
     CDS             <1..263
                     /locus_tag="PLU43_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28606.1"
                     /translation="ADIYTPIKWRCADGHEFMLSVNAVLQGGHWCPECLKKEWRYGDI
                     AKKNPFYAQVWFPLHGEEDNYVIPMEYSAYDVREELKEKLGL"
     gene            complement(335..970)
                     /locus_tag="PLU43_09090"
     CDS             complement(335..970)
                     /locus_tag="PLU43_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO28607.1"
                     /translation="MTRKIIKINEEQCNGCGLCAKACHEGAIGMVNGKARLLRDDYCD
                     GLGDCLPACPTGAISFEEREAAEYDQAAVNRHKKTKQESVPVDILKNKSRLSQWPVQI
                     KLAPVSAPYFSNTDLLIAADCTAYAYDDFHNSFIKNKITLIGCPKLDKQDYSEKLTEI
                     FKNNSIRSITVVKMEVPCCRGIEIAVEKAIRNSEKSIPLQIITISTDGRIL"
     gene            1072..>1687
                     /locus_tag="PLU43_09095"
     CDS             1072..>1687
                     /locus_tag="PLU43_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOO28608.1"
                     /translation="MNQYLHILKTAELFQGIEETELLLLLSCLSARVLFYEKGQTVFL
                     AGERITQFGLVLSGQVQIVQDDYYGNRSILGKMETGDLFGESFVYANIKVLPFSVITA
                     AESEILFIDGYRLAAPCARACGFHSMLIRNMLHIVSMKNVMLTQKIEFISKRTTREKL
                     LAYLSAEAKKAGSGSFFIPFNRQELADYLSVERSAMSAELSKLRN"
BASE COUNT          436 a          317 c          436 g          498 t
ORIGIN      
        1 ctgcggatat ttacaccccg atcaaatggc gctgcgcgga cggacacgaa tttatgctgt
       61 cggtaaatgc ggtattgcag ggaggacact ggtgtccgga atgtctgaaa aaggaatgga
      121 gatatggaga tattgcgaaa aagaatccat tttatgcgca ggtgtggttc ccgctgcacg
      181 gagaagagga caattatgtg attccaatgg aatacagtgc atatgacgtc agagaagaat
      241 taaaagaaaa gcttgggtta taaatggctt gagacagtac agatgagaag atcagaaaaa
      301 gaggtgtcca aaagacaaag gacacctctt ttttttaaag gatccggccg tctgttgaga
      361 tcgtgatgat ctgcaggggg attgatttct cgctgttccg gatggctttt tcgacagcga
      421 tttcaatacc tctgcagcag ggaacttcca tttttacgac agtaatgctc cggatgctgt
      481 tgttcttgaa tatttctgtc agcttttcac tgtaatcctg tttatcgagt ttgggacatc
      541 cgatcagcgt gattttgttt ttaatgaaac tgttgtgaaa atcgtcgtag gcatatgccg
      601 tacagtcggc ggcgatcagc agatcagtgt ttgaaaaata gggcgcgctg acaggagcaa
      661 gtttgatctg gaccggccat tgagataagc ggcttttatt tttcaggata tcaacgggaa
      721 cagattcctg tttggttttt ttgtgtctgt tcactgcggc ctggtcatat tctgcagctt
      781 cccgttcttc aaagctgatt gcacctgttg gacaggccgg cagacagtct ccgagtccgt
      841 cacagtaatc atcacgcaga agccgtgctt tcccgtttac cataccgatg gcaccttcgt
      901 ggcaggcttt tgcgcacaag ccgcagccgt tacattgttc ttcgttgatt tttattattt
      961 ttcgagtcat aaaatagtct ccttttttgc atgcatgatc attgacttta tgaaaaaagt
     1021 ttactatgat tgaagagatg agtctgttgg atttccaacg aaaggggttt tatgaatcaa
     1081 tatctgcata tattaaaaac agcggagctg ttccagggga ttgaagaaac agagctgctg
     1141 ctactgctgt cttgtctgtc tgcaagggtt ctgttttatg aaaaggggca gactgtgttt
     1201 ttagctggtg aaaggatcac acagtttggt cttgttctgt cgggacaggt gcagatcgtg
     1261 caggatgatt attatggaaa ccgaagcatt cttggaaaaa tggaaacagg ggatctgttt
     1321 ggggaatctt ttgtatatgc aaatataaaa gtgcttccgt ttagtgtgat cactgcagca
     1381 gaaagtgaga ttctttttat cgatggatac aggcttgctg cgccatgtgc cagagcatgc
     1441 ggttttcaca gcatgctgat ccggaatatg cttcatattg tgtcaatgaa aaatgtcatg
     1501 ctgacgcaga agatcgagtt tatttcaaag cggacgaccc gggaaaagct gcttgcatat
     1561 ctttctgcgg aggcaaagaa ggcagggagc ggaagctttt ttattccgtt caatcgtcag
     1621 gaacttgccg actatctttc tgttgaacgc agtgcgatgt ccgcggagct ttccaagctt
     1681 agaaatg
//
LOCUS       DAOSYE010000548         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62287_length_1684_cov_3.387354, whole genome shotgun sequence.
ACCESSION   DAOSYE010000548 DAOSYE010000000
VERSION     DAOSYE010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62287_length_1684_cov_3.387354"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PLU43_09100"
     CDS             <1..1390
                     /locus_tag="PLU43_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28609.1"
                     /translation="EILRIEHARKIIFDKHILNDFKINVLDSEIVSLCGVRAFENDLL
                     LDFFEGRTSLDKGRFYFMEKFYDITRQTLHLNGQILCIRKKPCLVQQLSVGENLFVVK
                     HNARKFFLNRKAVYQQTAYLLSKYKLSLPPKSRIQDLSIADQHIVELIRASIQNIRLI
                     VLEDIAMSYSESERQMLLHTVRLLQKQGIALIYLSFYPSCITDISSRILLMYAGSHLR
                     TLYPGECTLQELTGIINRQHPDPSSFINRQYTGKEIFVIAGLQYGAGIPALNLQLHEG
                     EILGIYDNEALFAKKIVEAILITGTGGNTFRLSGKIYTPQGLPHAVQSGIVYIPNFFY
                     RDSYLDNLSGSQNLSLPLIRKTRRAGLLINRHIEDYSWSKLEELLNDYFPEDSIDGIY
                     KEICIVYYHWILYRPKIIVCLDPFSYGDSLIRKIITVFLRMAVSMDIGILLLSHDYND
                     LTQLSDRVIRIT"
     gene            1467..>1684
                     /locus_tag="PLU43_09105"
     CDS             1467..>1684
                     /locus_tag="PLU43_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28610.1"
                     /translation="MKEHKKTPSTEYQGSGSSANHDVFTLAASLRWNSDAENLQNGIG
                     SASSGAVRYVETSEGPVRVLEYGFSNLSV"
BASE COUNT          457 a          388 c          330 g          509 t
ORIGIN      
        1 agagattctg agaattgaac atgcccgaaa aatcattttc gacaaacata ttcttaatga
       61 ttttaaaata aatgtattgg attctgaaat cgtttcgcta tgtggcgtac gggctttcga
      121 aaatgatctt ctgctggatt tttttgaagg ccgcacttct ttggataaag gacgcttcta
      181 ttttatggaa aaattttatg atattacccg ccaaacactg catttgaatg gccaaatcct
      241 ctgtatacgc aaaaagccct gtctcgtaca gcaactatct gtcggtgaaa atttatttgt
      301 tgtcaaacac aatgcccgaa aattttttct gaaccggaaa gctgtatatc agcaaacggc
      361 ctatcttctt tctaaatata agctgtccct gcctccaaaa agccgtattc aggatctttc
      421 cattgccgac cagcatattg ttgaactgat tcgagcctct attcagaata tccggctgat
      481 cgttctggaa gatattgcca tgtcttattc cgaatctgag cggcagatgc tgctgcatac
      541 cgtacgtctt ttgcagaaac aggggatcgc tctgatttat ctatcttttt atccatcctg
      601 cattacggat atttccagtc gaattcttct gatgtatgcg ggatcgcatc tacgcacgct
      661 ttatccgggt gaatgtacac ttcaggaatt aacaggcatc atcaaccgcc agcacccgga
      721 tccttcttca tttataaacc ggcaatatac aggcaaagaa atatttgtta ttgcagggct
      781 ccagtacggt gccggtatac cagctttaaa tcttcagctc catgaaggtg agattctggg
      841 aatttatgac aatgaggccc tgtttgcgaa aaaaattgtc gaagcaatcc ttatcaccgg
      901 taccggcggc aacaccttcc gactaagcgg caaaatatat acaccgcagg ggcttcccca
      961 tgccgtccaa agcggcattg tatacattcc caatttcttt taccgggatt cctaccttga
     1021 caatctctcc ggctctcaaa atctgtcgct gccactgatt cggaaaaccc gacgcgccgg
     1081 tctgctcatc aaccgccata ttgaagatta ttcctggtcc aaactggaag aacttttgaa
     1141 tgactacttt ccggaagact ctatcgacgg catttataaa gaaatatgta ttgtttacta
     1201 tcactggatt ctttaccgcc cgaaaatcat tgtatgtctg gaccccttct catacggtga
     1261 ctctttgatt cgaaagatca ttactgtttt tttgagaatg gccgtctcta tggatatagg
     1321 catccttctt ctctctcatg attacaatga tctgactcag ctgtctgacc gggttatacg
     1381 tatcacataa tgcacccttt tagggaaata ctaatttgtt ttagaaagga gctgcagaat
     1441 agtttctcta tcctgcaaaa aacgaaatga aagaacataa aaaaacacca tctactgaat
     1501 atcaggggag tggatcttcc gcaaaccatg atgtatttac tttggctgct tctcttcgct
     1561 ggaattccga cgccgaaaat cttcaaaacg gtattggatc cgcatcctcc ggtgcggttc
     1621 gatatgtgga gacttccgaa ggaccggttc gggttttgga atacggattc tcgaatcttt
     1681 ccgt
//
LOCUS       DAOSYE010000549         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62378_length_1683_cov_2.178133, whole genome shotgun sequence.
ACCESSION   DAOSYE010000549 DAOSYE010000000
VERSION     DAOSYE010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62378_length_1683_cov_2.178133"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1350)
                     /locus_tag="PLU43_09110"
     CDS             complement(103..1350)
                     /locus_tag="PLU43_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOO28611.1"
                     /translation="MLKLFPGKHTTNQNSHSFFKYIGPGLLVTVGFIDPGNWASNIAA
                     GSQYGYVLLWMVTLSTVMLIILQHNAAHLGIVTGKCLSEAATEYMRPWIKNIILLTAV
                     GAAISTALAEILGASMALNMLFKLPIRIGAILVLVVVLVLQFTNSYKKIEKIIIGFVS
                     LIGISFLFEIFMVHNDWNAAVKGWIVPSFPTGSMLIIMSVLGAVVMPHNLFLHSEIIQ
                     SRQWNLKDEKISQEQLKYEFYDTLFSMIIGWAINSAMILLAAATFFTRKIVVTDLSQA
                     EAMLSPLLGNMAGIIFAVALLFAGLSSSITAGMAGGSIFAGIFAEPYDIKDSHTKSGV
                     LLTMIPATLIIFLISSPFDGLIYSQMLLSIQLPITIFALIYLTSSKKVMGKYANSKGN
                     KTALWCIGLIVTILNIALLVSYL"
BASE COUNT          520 a          340 c          361 g          462 t
ORIGIN      
        1 attatgggga tgaccaaaaa tttaggcatg attgaagaaa ttcttcttta ttctaaaagc
       61 agtattctgt ttatttcttc agagggcttt gaaaatgcgc tgttataaat aacttactag
      121 aagcgcgata ttcagaattg tcacgatcaa tccaatgcac cacagcgcag ttttattacc
      181 ttttgaattt gcgtatttgc ccataacctt ttttgaggaa gtgagataaa taagcgcaaa
      241 aatggtaatg ggaagctgta tgctcaaaag catttgagaa taaatcagac cgtcaaacgg
      301 agaagatata agaaagatga tcagtgttgc cggtatcatg gtgagcagaa ctccactctt
      361 tgtgtgactg tcttttatat catagggttc tgcaaagata ccggcaaaga tactgccgcc
      421 ggccatacct gcagttatac tggaagacag tccggcgaaa agcaaagcga cggcaaatat
      481 gatacctgcc atgtttccca acaaggggct tagcatggct tccgcctgtg acagatctgt
      541 tacaacgatt tttcgggtaa agaaagtcgc agctgccaat aaaatcatgg cgctgtttat
      601 agcccagcca atgatcatgg aaaacagtgt gtcgtaaaat tcgtatttca attgttcctg
      661 acttattttt tcgtccttca gattccattg cctgctctgt atgatttcag aatgtaaaaa
      721 caggttgtgc ggcatgacca ccgcgccaag cacactcata ataataagca tggaacctgt
      781 tggaaatgaa ggcacgatcc accctttgac tgcagcgttc caatcgttat gcaccataaa
      841 gatctcaaaa aggaacgaga tcccaatcag tgaaacaaac ccgataatga ttttttctat
      901 ttttttataa gaatttgtaa actgaaggac caaaacaact acgaggacaa ggattgcccc
      961 gattctgatc ggcagtttga aaagcatgtt aagcgccatg gaagcgccga ggatttcagc
     1021 aagtgccgtt gatattgccg ctccaacagc cgttagtaaa atgatatttt tgatccacgg
     1081 ccgcatgtat tcggtagctg cctctgaaag acattttccg gttacgatgc cgaggtgtgc
     1141 cgcattatgc tggagaatga taagcatgac agtggaaagc gtaaccatcc acaaaaggac
     1201 ataaccatat tgtgagccgg ctgcgatatt ggaagcccag ttaccggggt caatgaaacc
     1261 gacggtgacc agcaggccgg gacctatata tttaaaaaaa ctgtgtgaat tttgatttgt
     1321 tgtatgttta cctggaaata atttcaatat ttatcacctt tctttatccc tgcaggaaaa
     1381 tgcacggcag gcttttacgc aacaaatcgt accacctgat atagccggtg tcaagtattc
     1441 cgccgcatca ggcagcattt gtttacgatg tcaaaatact gctgaaattt atcgcagatt
     1501 aatcagctgt tttccgatgt tgaaaggagt gtgttataat acagtcataa gtggtccgga
     1561 gtgaaacgta acattaaagc gccggaataa aaataagaaa gaggaatgag tatgccccca
     1621 ttaagtgtat tactcaaact tcgcacatag attttagcca tgcaccttga aaattcaata
     1681 cct
//
LOCUS       DAOSYE010000550         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62464_length_1681_cov_3.631611, whole genome shotgun sequence.
ACCESSION   DAOSYE010000550 DAOSYE010000000
VERSION     DAOSYE010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62464_length_1681_cov_3.631611"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLU43_09115"
     CDS             <1..780
                     /locus_tag="PLU43_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOO28612.1"
                     /translation="QAAVAKNIRMLTTGSRILEKSKHLRVQDAYALRCVPQVHGAIRD
                     ALSYVRHTLDIELNSVTDNPIVFLDNGDVISGGNFHGEPMALAFDFLGIAVSEIASIS
                     ERRLERMVNGALNNGLPEFLTKHGGLNSGFMICQYCAASMVSENKVLAHPASVDSIPS
                     SANQEDHVSMGTTAARKLREIVENALSVLAFEFMAAAQGMDLREDVPSPVSQEVKELI
                     REKLPMLEEDREMRQDVEKMNRLVRSGEIVSLVCGLVPEFS"
     gene            800..>1681
                     /locus_tag="PLU43_09120"
     CDS             800..>1681
                     /locus_tag="PLU43_09120"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HOO28613.1"
                     /translation="MKYKINADRLMRNLFEMAKIGMNENGGIDREFGSEADAETREWL
                     KEYWNKNIGLTAATDAIANLRIQRSGTEALLPIVMGSHHDAVPDGGKYDGAMGVLIAT
                     EIMQTIAENEIKLRHPFQVISFTGEEPSPFNLSTLGSKVICGKLKKEDLFDCRHRETG
                     ELLSDAIGKVGGDIAQLEEARLQKGAVAAFLEAHNELGGYLEEEGLSIASVSHITGIY
                     REEIRIYGEANHAGTTMMQNRKDAMLALSELALKIEAAAKEFHDPNVVATVGYVKIIP
                     NEANIIAGEAKAIVDIRT"
BASE COUNT          487 a          303 c          491 g          400 t
ORIGIN      
        1 caggcagcag tggctaaaaa tatcagaatg cttacaaccg gcagcaggat tttggaaaaa
       61 tcaaaacatc tgcgtgtaca ggatgcatat gcgcttcgct gtgttccgca ggtgcacgga
      121 gcgatccgtg atgcactttc ctatgtaaga catacgctgg atattgaatt gaattccgtg
      181 acagacaatc cgatcgtgtt tttagacaat ggagatgtta tttcgggcgg gaattttcat
      241 ggggagccga tggcgctggc ctttgacttt ttaggaatcg ctgtttccga gatcgcaagt
      301 atttcggagc gccgtctgga acggatggta aacggagctt taaataacgg acttccggaa
      361 tttttaacaa agcacggcgg tctgaattcc ggttttatga tatgtcagta ttgtgcggcc
      421 agtatggtga gtgaaaataa agtactggca catccggcaa gtgtcgattc tattccgagt
      481 tctgcaaatc aggaagatca tgtcagtatg gggacgacag ccgcaaggaa attaagagag
      541 attgtggaaa atgcgctttc tgtacttgcc tttgaattta tggcagcagc ccagggaatg
      601 gatttgagag aggatgttcc ctcacctgtc agtcaggaag taaaggagct gatcagggag
      661 aagctcccta tgctggagga agacagggaa atgcggcagg atgttgagaa aatgaaccgg
      721 cttgtcagaa gcggggagat cgtttcactt gtatgcgggt tagtaccgga atttagttga
      781 aagtgaggtg acagatgaaa tgaaatacaa aatcaatgcg gatagattaa tgcggaatct
      841 gtttgaaatg gcgaagatcg gaatgaatga aaacggcggg atcgaccgtg aatttggaag
      901 tgaggcggat gccgaaacaa gggaatggct aaaagaatac tggaataaaa atattgggtt
      961 gactgcggca acggatgcga tcgctaatct gcggatacag aggagcggta cggaagcatt
     1021 attgccgatc gtcatgggtt cacatcatga tgcggtaccg gacggcggca aatatgacgg
     1081 tgcgatgggt gttctgatcg caacggagat catgcagaca attgcggaga acgagatcaa
     1141 gctgcgccat ccgtttcagg tgatctcatt taccggagag gagcccagtc cgtttaattt
     1201 atcaacgctt gggagcaaag tgatctgcgg aaagctgaag aaagaagacc tttttgactg
     1261 caggcatagg gaaacaggag aactcctttc tgatgcaatc ggtaaagttg gcggagatat
     1321 tgcgcagctt gaggaagcca ggctgcaaaa aggtgcggtg gcagcatttt tggaggcgca
     1381 taatgagctt ggcggatatt tggaggaaga gggactttcc attgcttccg ttagtcatat
     1441 tactggaatc taccgggaag agattcgaat ttacggagag gccaatcatg caggaaccac
     1501 aatgatgcaa aaccgaaaag atgcgatgct cgcattgagt gaactggcgc tgaaaataga
     1561 agcggcagca aaagagtttc atgatccgaa tgttgtcgca acggtcggtt acgttaagat
     1621 cattccgaat gaagccaata ttatagcagg ggaagcaaag gcgatcgttg atattcgtac
     1681 c
//
LOCUS       DAOSYE010000551         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62476_length_1681_cov_3.043665, whole genome shotgun sequence.
ACCESSION   DAOSYE010000551 DAOSYE010000000
VERSION     DAOSYE010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62476_length_1681_cov_3.043665"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLU43_09125"
     CDS             complement(<1..478)
                     /locus_tag="PLU43_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO28614.1"
                     /translation="MNQTAALVLKIVTAIVLIAAVMFGVYYGVSYFSDSNKMAEELEI
                     GNQYMEAEEYQAAIDAYDQALEYEPENEEVKSAIAHAYVMLAGTYGETDEAVEAYQKA
                     LLYNIENKNAYWGVANIYEGREDDENVIAALETGYENTQDENMKIKLDNIEAERAAA"
     gene            complement(519..1196)
                     /gene="ung"
                     /locus_tag="PLU43_09130"
     CDS             complement(519..1196)
                     /gene="ung"
                     /locus_tag="PLU43_09130"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO28615.1"
                     /translation="MAAIENDWLDSLQTEFKQPYYKSLYLKVKKEYSTRQVFPHPDDI
                     FNAFSMTPLSEVKVLILGQDPYHDTGQAHGLCFSVKPDVEIPPSLVNIYQELHDDLGC
                     YIPDNGYLEKWARQGVMLLNTVLTVRAHQANSHRGIGWEEFTDAAIRTLNEQDRPIVY
                     MLWGRPAQMKSGMLNNPKHLILKAPHPSPLSAYRGFFGCRHFSMANEFLKKNGLEEID
                     WQIENKK"
     gene            complement(1199..>1681)
                     /locus_tag="PLU43_09135"
     CDS             complement(1199..>1681)
                     /locus_tag="PLU43_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOO28616.1"
                     /translation="EGADKVSINSSAINRPELISEAADKFGSQCVVVAIDARRRADGL
                     GWNIFKNGGRIDTQIDAVEWAIKANELGAGEILLTSMDCDGTKAGYDLELTKIISENV
                     SIPVIASGGAGNKEHFYEALTKGKADAALAASLFHYKELEINDLKNYLSERGISIRRS
                     "
BASE COUNT          416 a          433 c          287 g          545 t
ORIGIN      
        1 gtgcggcggc gcgttctgcc tcaatattat ccagcttgat tttcatattt tcatcctgcg
       61 tattttcgta gccggtctct aaggccgcaa taacattttc atcgtcttcg cgtccttcat
      121 aaatatttgc tactccccaa taagcatttt tattctcaat attataaaga agcgctttct
      181 gatatgcctc aaccgcttca tctgtttctc cataagttcc tgcaagcatt acataggcat
      241 gtgcgattgc actcttcact tcctcatttt ccggctcgta ttccaacgcc tggtcatatg
      301 cgtcgatcgc tgcctgatat tcttcagctt ccatatactg attgcctatt tcaagttctt
      361 ccgccatttt gttggaatca gaaaaataac tgactccata ataaacaccg aacattactg
      421 ctgctataag gacaatggct gtcacaatct taagaacaag cgccgctgtc tgattcattt
      481 caaaactcct cacctttttt aatcaatcat atctatcttt attttttgtt ttctatctgc
      541 cagtcgatct cttctaaacc attctttttt aaaaactcat ttgccatact aaaatgcctg
      601 catccaaaaa atcctctgta tgccgataat ggacttggat gaggtgcttt taaaatcaag
      661 tgctttggat tattcagcat accacttttc atttgtgccg gccttcccca aagcatataa
      721 acgattggtc tgtcctgttc gtttagagtt ctgatcgccg catcggtaaa ttcttcccac
      781 ccaataccac gatgggaatt tgcctgatgt gcacggacag tcagcaccgt attgagcagc
      841 ataactccct gtcttgccca tttttccaaa taaccgttat ccggaatata acatcccaga
      901 tcatcatgca gttcctgata aatattaaca agtgacggcg ggatctctac atccggcttt
      961 accgaaaaac aaagaccatg tgcctgtccg gtatcatggt acggatcctg cccgagtatc
     1021 agcactttga cttcgcttag cggtgtcatg gaaaatgcgt tgaaaatatc atccggatga
     1081 gggaaaacct gtcttgtcga atattctttt tttactttta aatacaggct tttataataa
     1141 ggctgcttaa attccgtttg cagcgaatcc agccagtcat tttcaattgc cgccattttt
     1201 atgatctcct tatcgaaatt cctctttcac ttaaataatt cttcagatca ttgatttcca
     1261 gttctttata atgaaacagg gatgctgcca aagccgcatc agcctttcct tttgtcaatg
     1321 cttcataaaa atgctcttta ttgccggccc cgccggaagc aatcacagga attgatacat
     1381 tctccgatat gatctttgtg agttccagat catagccggc ctttgtcccg tcgcagtcca
     1441 tacttgtaag caggatctca cctgcgccga gttcattcgc tttgatcgcc cactctacgg
     1501 catcaatctg agtatcgatc cgtccgccat ttttaaaaat attccatccc aaaccgtcgg
     1561 ctctccgtct tgcatcgatt gccaccacca cacactggct gccgaatttg tcggcggctt
     1621 cactgatcag ctccggacga ttgattgctg aggaattgat tgaaacttta tccgctcctt
     1681 c
//
LOCUS       DAOSYE010000552         1680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62552_length_1680_cov_2.513846, whole genome shotgun sequence.
ACCESSION   DAOSYE010000552 DAOSYE010000000
VERSION     DAOSYE010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62552_length_1680_cov_2.513846"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1680)
                     /locus_tag="PLU43_09140"
     CDS             complement(<1..>1680)
                     /locus_tag="PLU43_09140"
                     /inference="COORDINATES: protein motif:HMM:NF037639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HOO28617.1"
                     /translation="STQNENKSYDFENTKIQAGVKFLKVDADHELLGGAVFHLYRIET
                     DGTIVDFGTVTATANGVVEKQGLGAGSYYFVEETAPTGYVDNTKQYTFTITAADNGTT
                     VGLSNPDVTVDEIGAVVNQPKTGDARLTKVIKGTNTGLAGAKFALYAKGNDTPIKTDL
                     ESDANGYVTVTGLVWGEYYFMETSAPSGYALDSDTKYEFEVSADNVSSLIEVKTKDGG
                     NAENELILGKAQLVKYDADTEDVLSGALFDVYDADTQTIVSGYESGILSDVNGVVETN
                     KDLPEGSYYFKERTAPTGYALDTTEYSFTITQDNMDTTVTAGTDGKAYNTPDHGKAEL
                     FKYVLGEDGYTKTGLEGAVFKLYKVNSLGPISWKTEIDTYTTDANGIITVTDLLWGEY
                     YFQEETAPTGYERKTGDAGKISFTISATQLDYTGTARFSMSNDDYKGSIKLVKTESGT
                     GDEIEGAVFRLYQISGGTTTEIKNATVSDGTYTTDKNGEINVTDLDWASYYFVEISAP
                     TGYAMPADPTTSTVTITKDNVKASIESPLTIEAENTKVYGNVKLTKIDDATP"
BASE COUNT          378 a          441 c          321 g          540 t
ORIGIN      
        1 ggtgtcgcat catcaatctt tgtcagcttc acattaccat agacctttgt attttccgcc
       61 tcaatcgtaa gaggtgattc aatggatgct tttacattgt cttttgtaat cgtcactgtc
      121 gatgtcgttg gatcagccgg cattgcataa ccggtcggcg cactgatttc tacgaaatag
      181 tagcttgccc agtcaagatc cgttacattg atctcaccgt ttttatctgt cgtataagta
      241 ccgtcagata cggttgcatt tttaatttca gtcgtcgtac cgcccgatat ctgatacagt
      301 ctgaagaccg caccttcgat ctcgtctccg gttccgctct ctgtctttac aagcttgatc
      361 gaacctttat agtcgtcatt tgacatggag aatctggccg ttccggtata atccagctgc
      421 gttgcgctga tcgtaaagga gatctttcca gcgtctcctg tcttcctttc ataaccggtc
      481 ggtgctgttt cttcctggaa ataatattct ccccatagca gatccgttac ggtaatgatt
      541 ccattcgcat ctgttgtata agtatctatt tccgtcttcc aggaaatcgg tcctaatgaa
      601 ttaaccttgt acaatttgaa aactgcacct tccagaccgg tctttgtata accgtcttca
      661 ccaagtacat acttgaacag ctcagccttg ccgtgatcag gcgtattgta tgctttgccg
      721 tccgtgcctg cggttaccgt tgtatccata ttatcctgtg tgatcgtaaa ggaatactct
      781 gtcgtatcaa gtgcatagcc ggtcggggcc gtacgctcct tgaaataata actgccttcc
      841 ggtagatctt tgtttgtttc aacaacaccg ttcacatccg aaaggatccc gctctcatat
      901 cctgatacga tcgtctgtgt atcagcatca tatacatcga acaatgcacc ggaaagtaca
      961 tcttccgtat cagcatcata tttcacgagc tgtgcttttc cgaggatcaa ttcattctca
     1021 gcgtttcctc cgtctttcgt cttgacttct atcagacttg acacattatc cgcgctgact
     1081 tcaaattcat attttgtatc ggaatccagc gcatatccgc ttggagccga tgtctccata
     1141 aagtaatact caccccatac aagaccggtt acagtcacat acccgtttgc atcggactca
     1201 agatctgttt tgatcggtgt atcgtttcct tttgcataaa gcgcaaactt cgcacccgcc
     1261 aaaccggtat tggttccttt gatcaccttg gtcagtctgg catccccggt tttgggctga
     1321 ttaacaacag ctccaatttc atccactgtt acatccggat tgctcagacc tactgtcgtt
     1381 ccgttatcgg cagctgttat cgtaaatgta tactgctttg tattatccac atagccggtc
     1441 ggtgctgttt cttctacaaa atagtagctg cctgcaccaa gaccctgctt ttcaacgaca
     1501 ccattcgcgg tcgccgttac agtaccgaaa tctacaattg taccatccgt ctctattctg
     1561 taaagatgga atacggctcc tccaagcagc tcgtgatccg catctacctt caggaatttt
     1621 actccggcct gtatttttgt attttcaaag tcgtaactct tattctcatt ctgcgtcgag
//
LOCUS       DAOSYE010000553         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62656_length_1678_cov_4.554529, whole genome shotgun sequence.
ACCESSION   DAOSYE010000553 DAOSYE010000000
VERSION     DAOSYE010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62656_length_1678_cov_4.554529"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /gene="aspS"
                     /locus_tag="PLU43_09145"
     CDS             <1..1087
                     /gene="aspS"
                     /locus_tag="PLU43_09145"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOO28618.1"
                     /translation="RQPEFTQIDLEMSFADVDDVIAVNEGMIQNLLKDILNVDIRLPI
                     ERMTWIDAMNRFGSDKPDLRFGMELTDVSDLVKDCGFGVFSGAVGNGGTVRGINVKGE
                     AEMPRKKIDALTEFAKGYGAKGLAYLCVRPDGTYKSSFAKFMTEEELQALVKAMNGEA
                     GDLLLFAADRTKIVWDVLGALRIELAKTLCLIPKGEFRFLWVTEFPLLEWSEDEARFT
                     AMHHPFTMPMDEDICYIDTDPGRVRAKAYDIILNGTELGGGSVRIHQQDIQEKMFEVL
                     GFTKEDAYERFGFLLNAFQYGVPPHAGLAYGLDRLVMLMTGAESIREVIAFPKVKDAS
                     CLLSGAPNVVDAKQLAELQIKIDSEEE"
     gene            1102..>1678
                     /locus_tag="PLU43_09150"
     CDS             1102..>1678
                     /locus_tag="PLU43_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28619.1"
                     /translation="MVKKLNGVIEKFILLVYVLMVVFILFVQKDIKVVIWIYAALLFS
                     IALMVFLTFCPRVSNDTRGIVMAAALTMLTGVYCYTTSTENVFNIVLLMIACLMSLYC
                     SMKVIRFLFMISFVFYLFYLVFSFGALKLEGVLQICALFGGQVALVLLLKKNELTERI
                     NRQKEQSNADLLRVVEIKKRDAEAASRAKADF"
BASE COUNT          430 a          315 c          478 g          455 t
ORIGIN      
        1 cagacagccg gaattcacgc agatcgatct ggaaatgtca tttgcggatg tggatgatgt
       61 gatcgcggtc aacgaaggaa tgattcagaa cctgctcaaa gatattttaa atgttgatat
      121 caggcttccg atcgaacgca tgacgtggat agacgccatg aaccgtttcg gatccgataa
      181 accggatctg cgttttggca tggagctgac cgatgtgtcc gatcttgtaa aggactgcgg
      241 gttcggcgta ttttcaggag cagtcggaaa cggcggaacc gtgcgtggaa tcaatgtaaa
      301 gggcgaggca gaaatgccaa ggaagaaaat agatgcgctg acggaatttg caaagggata
      361 cggggcaaag ggactggctt atctctgtgt ccggccggac gggacatata aatcttcatt
      421 tgccaaattt atgacagaag aagaattgca ggcacttgtg aaggccatga acggggaagc
      481 cggagatctg cttttgtttg ctgcagaccg gacaaagatc gtatgggacg tgcttggtgc
      541 ccttcggatc gaactggcga aaacactttg cctgattcca aaaggggagt tcaggttttt
      601 atgggtaacg gaatttccgc tgcttgagtg gagcgaggat gaagcgcgtt ttactgccat
      661 gcatcatccg tttacgatgc cgatggacga ggatatttgc tacattgata ccgatccggg
      721 aagggtgcgg gcaaaagcat atgatattat attgaatggt acggagcttg gaggcggaag
      781 cgtaaggatc catcagcagg acatccagga gaagatgttt gaggtgcttg gatttacaaa
      841 agaggatgcg tatgaacggt ttggatttct tttaaatgct tttcaatatg gagtgccgcc
      901 gcatgcggga cttgcttacg gcctggaccg tcttgtcatg ctgatgacag gagcggaaag
      961 catccgcgag gttattgcat ttccaaaagt aaaggatgct tcctgtctgc tgtcgggagc
     1021 accgaatgtc gttgatgcaa agcagcttgc ggagctgcag attaaaatcg attctgaaga
     1081 agaataggaa cggaacaggg tatggttaag aaactgaacg gcgtcattga gaagtttata
     1141 ttgctggttt atgtgcttat ggtcgtattc atattattcg tccaaaagga tatcaaggtg
     1201 gtcatatgga tctatgcggc acttttgttt tctattgcgc tgatggtttt tcttaccttc
     1261 tgcccaaggg tcagcaatga tacgcgcgga attgtcatgg cagcggctct gactatgctc
     1321 accggtgttt actgttatac gacttctacg gagaacgtat ttaatatcgt acttctgatg
     1381 atcgcgtgtc tgatgtctct ttactgttcc atgaaggtga tccgtttcct gtttatgata
     1441 tcgtttgtat tttatttgtt ttatcttgtg ttttcattcg gcgcgctgaa gctggaaggt
     1501 gtgcttcaga tctgcgcact gttcggcgga caggtcgctc ttgtactgct gcttaaaaag
     1561 aatgaactta cggaacgcat aaacagacag aaggaacaga gcaatgcgga tctgctgcgt
     1621 gttgttgaga tcaagaaaag ggatgcggaa gcagcatcaa gggcaaaggc ggactttc
//
LOCUS       DAOSYE010000554         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62834_length_1675_cov_4.020988, whole genome shotgun sequence.
ACCESSION   DAOSYE010000554 DAOSYE010000000
VERSION     DAOSYE010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62834_length_1675_cov_4.020988"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLU43_09155"
     CDS             complement(<1..413)
                     /locus_tag="PLU43_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28620.1"
                     /translation="MVLLIGLYAFLTNKTVAGRQIYALGGNRKAANLSGINTNKVFFW
                     VYTNMGILSAVAGIILSARNGSSTPKAGDGFEMDAIASCYIGGAAVAGGAGTIIGAVV
                     GAFVMGILNNGMSLFGWSTDIQKIVKGAVLLGAVTV"
     gene            complement(416..1138)
                     /locus_tag="PLU43_09160"
     CDS             complement(416..1138)
                     /locus_tag="PLU43_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28621.1"
                     /translation="MNKKKTVNLDMKQYGMFLALIAIYLIFAMLTGGKNLTPMNINNL
                     VMQNGYVVILAIGMLLCVLTGNVDLGVGSIVALTGASAGIIMMDYKMNMWAAILVALF
                     IGFLVGMFAGFFIAYLGIPPFVVTLATMLMGRGLTYTLLQAQTKGPLPESYNFIGAGF
                     LPTIKVPFGDGTLDLVSIAVAIIASLAMILTEIKNIATKKKYGFATNPVWQVGLKLAG
                     MLFIIWFFSINCPAITDFRLYF"
     gene            complement(1131..>1675)
                     /locus_tag="PLU43_09165"
     CDS             complement(1131..>1675)
                     /locus_tag="PLU43_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28622.1"
                     /translation="DAIDNKFSYVSEDRKTYGLILINDIKENMTLAARPRFFSKRGVI
                     NGNDEIMAAEEYKKKINVKANSINQIVGSLSGGNQQKVVLAKWMMTKPDVLILDEPTR
                     GIDVGAKYEIYCVINELAKSGKAVIVISSEMPEIIGTCDRVYVINEGEIAGELTKEEV
                     SQEKIMQCIMAHNRKDDGNE"
BASE COUNT          480 a          428 c          318 g          449 t
ORIGIN      
        1 acggtaaccg cgcctaaaag tacggcacct tttacgatct tctgaatatc cgtagaccat
       61 ccgaacaaag acataccatt gttcagaata cccataacaa acgcaccgac aaccgcgccg
      121 ataatcgttc cggcaccgcc ggccacggca gctccgccga tataacagga agcgatggca
      181 tccatttcaa aaccgtcgcc tgcctttggt gtggaagaac cgttccttgc agacagaata
      241 atacctgcta ccgcacttaa gattcccata ttcgtatata cccagaaaaa cactttattc
      301 gtattgatac cggaaagatt tgctgctttt ctgtttccgc caagtgcgta gatctgacgg
      361 ccggctacgg tcttgttcgt aaggaatgca taaagaccga tcaggagcac catgatcaaa
      421 agtacaaacg gaagtccgtt atagcgggac agtttataga aaagaaccag atgataaaca
      481 gcatgccagc aagcttcagt ccgacctgcc atacgggatt tgtcgcaaat ccatatttct
      541 tctttgtagc aatatttttg atttccgtca ggatcatcgc cagggaagca atgatcgcta
      601 ccgcgatact gacaagatca agcgttccgt ccccgaacgg aacttttatc gtcggaagaa
      661 acccggcacc aataaaattg tagctctccg gaagcgggcc ctttgtctgg gcctgcagca
      721 gcgtataagt aaggcctctg cccatcagca tcgtagcaag cgttacaaca aacgggggaa
      781 tccccagata agcgataaag aaacctgcaa acataccaac cagaaatcca ataaagagag
      841 caactaaaat ggctgcccac atattcattt tataatccat cataatgatt ccggccgacg
      901 cgcctgtcag cgctacaata gaaccaacgc ccagatcgac atttcctgtc agtacacaga
      961 gcagcatacc aattgccaga atcacaacgt atccgttctg cattacaagg ttgttgatat
     1021 tcatgggcgt cagattcttg ccgcccgtca gcatggcaaa aatcaaatag atcgcaataa
     1081 gggcaagaaa catgccatac tgtttcatgt caagatttac tgtttttttc ttattcattt
     1141 ccatcatcct ttctgttatg agccataatg cactgcatga ttttttcctg ggatacttct
     1201 tcttttgtca gttcaccggc gatctcacct tcattaatga catacaccct gtcacatgtg
     1261 ccgatgattt ccggcatttc ggaagaaatt acaataactg ctttcccgga ctttgcaagc
     1321 tcatttataa cacaatagat ctcatatttg gcacctacgt cgataccacg ggtaggctca
     1381 tccagaatca atacatcagg ttttgtcatc atccactttg caagcactac cttttgctga
     1441 tttccgccgg acagagagcc cacgatctga ttgatggaat ttgcctttac attgatcttc
     1501 tttttatatt cttctgctgc cattatttcg tcatttccgt taataacacc tcttttggaa
     1561 aaaaatcgcg gacgtgccgc caaggtcata ttttccttga tatcattaat cagaatcaat
     1621 ccataggttt tacggtcttc agacacataa gaaaatttat tgtcaatggc atcct
//
LOCUS       DAOSYE010000555         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62860_length_1675_cov_2.627778, whole genome shotgun sequence.
ACCESSION   DAOSYE010000555 DAOSYE010000000
VERSION     DAOSYE010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62860_length_1675_cov_2.627778"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1302)
                     /locus_tag="PLU43_09170"
     CDS             complement(127..1302)
                     /locus_tag="PLU43_09170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOO28623.1"
                     /translation="MKRDAVIVSAVRTPVGKYGGVLAPVKDYELGIEVIREAVKRADI
                     PTEEIDDVYFGNLLGVPGNVAKVAAMGAGLPAHIPAVTIDRQCASGLESISIAAAMIK
                     SGMGDIYIAGGCESMTNRPYYMEKQSKLFDARPPRFLENMFAPPTGFEQITMGQTAEN
                     IFEDYEFSREELDQCSYESHMKAVKAIKAGNFREQIIPLEVPQKKRAILVDTDEGPRE
                     HTNMEQLGRLKPLFKENGKVTAGNSCPMNDGAAALVVMSREKAEAGGCKILASITDSC
                     AVGVDYRKMGLGPVYATRKLLEKTKIDKKEIGLVELNEAFAVQTLACLQELKFDKEIV
                     NVNGGAIALGHPLAATGAVLSTKILYEMKKRSTRYGLVTMCIGGGQGMAMLFKNEEK"
     gene            complement(1339..>1675)
                     /locus_tag="PLU43_09175"
     CDS             complement(1339..>1675)
                     /locus_tag="PLU43_09175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOO28624.1"
                     /translation="KGNLANWIVPGKTVPGMGGGMELAQKAKKVIILTRQTDKNGCSK
                     IRKECSLPITAPGCVDMIITDMAVYKVRKEGLMLTEIYEDITIEEVLEKTEANVIVAD
                     AVKKIPIGK"
BASE COUNT          346 a          478 c          289 g          562 t
ORIGIN      
        1 ttcttccaat cctaattccg gatgttccca aaggtaatgc gcagcttctt caaaatcagc
       61 cttattttta tcgatccact ccattatttt atttttttct tctgctgccg ccacttttat
      121 tcctccctac ttttcttcat ttttgaacag catggccatt ccctgtccgc cgccgataca
      181 cattgtgaca agcccatagc gcgtgcttct ttttttcatt tcatatagta tttttgtcga
      241 aaggacagcg cctgtcgccg ccaggggatg acccagcgcg atcgctccgc cgtttacatt
      301 tacgatctcc ttatcaaatt ttaattcctg cagacaggca agcgtttgaa cggcaaacgc
      361 ctcatttaat tctaccagtc caatctcctt tttatctatt ttcgttttct ccagtaattt
      421 tcgtgtagca taaacaggcc ctaaccccat tttcctgtaa tctacgccta ccgcacagct
      481 gtctgtaata gaagccagta ttttacatcc gcctgcttct gccttctccc tgctcatgac
      541 aaccagtgct gctgcaccgt cattcattgg gcagctgttt ccggcagtta cttttccgtt
      601 ttccttaaat aacggcttca gccttccaag ctgttccatg ttcgtatgtt ctcttggccc
      661 ttcatccgta tctacgagta tcgccctttt tttttgcgga acctccagcg gtatgatctg
      721 ttcccggaag tttccggctt ttattgcttt tacggccttc atatggcttt cataagaaca
      781 ctggtctaat tcctcacgtg aaaactcata gtcctcaaaa atattttcag ccgtttgtcc
      841 cattgtgatc tgttcaaacc ctgtcggcgg ggcaaacata ttttccagaa acctaggcgg
      901 ccttgcatca aacagttttg attgtttttc catataataa ggcctgtttg tcatgctctc
      961 acacccgccg gcaatataaa tatcacccat tcccgacttg atcattgcag ctgctatact
     1021 gatggattcc aggccggaag cacactgtct gtcaattgtt accgcaggaa tatgagccgg
     1081 cagacccgcg cccatggctg ccacttttgc cacattccca ggaacaccaa gtaaatttcc
     1141 aaaatatacg tcatctattt cttctgtcgg aatatccgct cgtttcaccg cttctctgat
     1201 tacttcaatt cccaattcat agtctttcac aggcgccaga acgcctccgt attttcctac
     1261 aggagtcctg actgccgata cgattaccgc atcccgtttc attctctgcc tcttttccgg
     1321 ccatgcagtc cgacaggctt acttgccgat cgggatcttc ttgactgcat ctgccacaat
     1381 tacgtttgct tctgtctttt ctaatacttc ttctatggta atgtcttcat aaatttccgt
     1441 taacataagg ccttcctttc tgaccttata taccgccatg tccgtaatga tcatatccac
     1501 acagccggga gccgtgatcg gcagactgca ttcctttctg atcttggagc atccgttttt
     1561 atctgtctgt cttgtcagta tgatcacttt ttttgctttt tgggccagct ccattccgcc
     1621 gcccattccg ggcactgttt ttcccggtac gatccaattg gccagattcc ctttt
//
LOCUS       DAOSYE010000556         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_62902_length_1674_cov_3.108091, whole genome shotgun sequence.
ACCESSION   DAOSYE010000556 DAOSYE010000000
VERSION     DAOSYE010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62902_length_1674_cov_3.108091"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..944
                     /locus_tag="PLU43_09180"
     CDS             3..944
                     /locus_tag="PLU43_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO28625.1"
                     /translation="MDIISGFLGAGKTTLIKKLLEEAFADENVVLIENEFGEVGIDGP
                     LLKEYGVEIREMNSGCICCTIAGDFSLALKELLAMYHPDRIIIEPSGVGKLSDVIGGC
                     KKYLKDGEVELNMCITVIDALKYRVYLKNFTEFYKNQLEHAKTIILSRTRNTSSKVIE
                     AVLADILNYNPKAHVVTTDWNDLSGRKIVDLAEIAEEIQGLSDLLKQSACGCAHEGGM
                     HHHADEVFRVWGTETAKMFTRDRLEEILHGLKDTEGTVLRAKGIVGTEDGSWLQFDYV
                     PEEESVRETIPECVGRICVIGERINEELLTELFEVQR"
     gene            947..>1674
                     /locus_tag="PLU43_09185"
     CDS             947..>1674
                     /locus_tag="PLU43_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO28626.1"
                     /translation="MTTYIDIVTGFLESGKTTFANELLQSSLLDEFEEIVLLICEEGF
                     TEYEEEKLSALQIRTVRLENREALNDELFSGLEAEYHPDYILVEYNGTWDISDLLGLK
                     IPLEYKFRNVVFVSNAGSFRNQLGNMAAVMQPHILNSDLVVFNRFENMQPQMQKKLAG
                     DIKNINPRTKAVFVGKIETDSILKEHFMSFSNPENTRLNFNMNVKAGIFLTICFALLV
                     LYELSGTYDFVQSVVKVSREELPRV"
BASE COUNT          485 a          286 c          464 g          439 t
ORIGIN      
        1 aaatagatat tatatcgggt tttttaggcg ctgggaaaac gacgctcata aagaagcttt
       61 tagaggaagc gtttgctgat gagaatgtcg ttctgataga aaatgaattc ggcgaagtgg
      121 ggatagacgg tcccctgtta aaggaatatg gtgttgagat cagggaaatg aattcaggat
      181 gcatctgctg tacgatcgcc ggagatttct ctcttgcttt aaaagaatta ctggccatgt
      241 atcacccgga caggatcatc atcgaaccgt ccggtgtagg gaagctgtct gatgtcatag
      301 gtggctgtaa aaagtactta aaggacggtg aggtagagct gaatatgtgc attacggtaa
      361 tagatgcact gaaataccgt gtttatctga agaattttac ggaattttat aaaaatcagc
      421 tggagcatgc aaagacgatc atattaagca gaacgcgcaa tacgtcgtct aaagtaattg
      481 aagctgtttt agcggatatt ttaaactata atccaaaggc acatgttgta acgacagact
      541 ggaatgacct ttccgggaga aagatcgtag atctggcaga gattgctgag gagatccagg
      601 gtctgagcga tctgctgaaa cagtctgcat gcggctgcgc gcatgagggc ggtatgcacc
      661 atcatgcgga tgaggtattt cgtgtctggg gaacagagac ggcaaaaatg ttcacgcggg
      721 acaggctcga agagatcctg cacggcctga aggatacgga aggaacagtt ttgcgggcaa
      781 aggggatcgt cggcacagaa gacggcagct ggctccagtt cgactatgtg ccggaagaag
      841 agtcggtcag ggaaacgata cctgaatgcg tcgggcggat ctgtgtgatc ggagaacgga
      901 taaatgagga gcttcttacg gagctgtttg aagtacagag gtaattatga cgacttatat
      961 cgatattgtg acagggtttc tggaaagcgg gaagactacg tttgccaatg aacttttaca
     1021 gagcagtctt ttggatgaat ttgaagaaat tgttttgctt atctgcgaag aaggctttac
     1081 cgaatatgag gaagaaaaac tgagtgcatt acagatccgg acggtccggc ttgaaaacag
     1141 ggaggcgtta aatgacgaac tgttttcagg gcttgaggcg gaatatcatc cggattatat
     1201 tcttgtggaa tataacggga catgggatat ttccgatctg cttggtctga agattccgct
     1261 tgaatataaa tttcgcaatg ttgtttttgt aagcaatgcg gggagcttca gaaatcagct
     1321 cggcaatatg gcggcagtca tgcagccgca tattttgaac agtgatcttg tcgtattcaa
     1381 tcggtttgaa aatatgcagc cgcaaatgca aaagaagctg gcgggcgata taaaaaatat
     1441 caatccgaga acaaaggctg tctttgtcgg taagatcgaa acggacagca tattgaagga
     1501 acactttatg tcattcagta atccggaaaa tacgagactg aattttaaca tgaatgtaaa
     1561 ggcgggcatt tttctgacga tctgctttgc cctgcttgtc ttatatgagc tttccggtac
     1621 gtatgatttt gtgcagtctg ttgtcaaagt atcgagggag gagttgccac gtgt
//
LOCUS       DAOSYE010000557         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63101_length_1671_cov_2.498144, whole genome shotgun sequence.
ACCESSION   DAOSYE010000557 DAOSYE010000000
VERSION     DAOSYE010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63101_length_1671_cov_2.498144"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..218)
                     /locus_tag="PLU43_09190"
     CDS             complement(24..218)
                     /locus_tag="PLU43_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28627.1"
                     /translation="MSNEIEYDYWWASMQSGFCARMKQIAEEAGGTKVLYRMDRDRLS
                     RLQGVSESFAAYIYNRRNIK"
     gene            complement(255..>1671)
                     /locus_tag="PLU43_09195"
     CDS             complement(255..>1671)
                     /locus_tag="PLU43_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOO28628.1"
                     /translation="SEVREVRERVRTALKNAGFLIPVKRVTVNLSPANVRKQGTGFDL
                     PVAVGILVAMGELSADAIKDTMIVGELLLSGRVGGICGALPLMLAAKKAGLRRCIIPK
                     ENEAEGAVVDGLEVICVESLIQLVAHLQGTSKIRPKQFAGEKLLSGIQNAGYDFSEVS
                     GQHFVKRGLEIAAAGLHNMIMIGSPGAGKTMLAKCIPSILPPLSMEECLEVSAVYSVA
                     GKLQGMRALITERPFVSPHHSVTATALSGGGIYPRPGSISLAHHGVLFLDELPEFSRS
                     ALEVLRQPLEERVVHVTRSKGSYTYPADFMLIGAMNPCPCGAYPDLGKCTCTETARKR
                     YLSRLSKPLLDRMDICTQVRQAKYRELVQKKDEECSAAVRGRVIQAQQRQYERFFEES
                     RCVRFNSGMGIKEIDRYCKLTAQGEKLLEDAFKKFDLSTRAYHRILRVARTIADLDNS
                     GQIREYHLAEAVHYKFNFEIQ"
BASE COUNT          386 a          505 c          314 g          466 t
ORIGIN      
        1 atttctccct ttcatttaat agttcatttg atatttcttc tattataaat atatgctgca
       61 aatgattctg aaacaccctg cagcctgctt agcctgtccc tgtccatccg atacagtact
      121 tttgttccgc ccgcttcttc ggcgatctgc ttcatacgcg cgcagaatcc gctttgcatg
      181 gaagcccacc agtaatcata ctctatctca ttactcatat tttccatatc ctttccacga
      241 tgtcttacac catttcattg tatttcaaaa ttaaatttat aatggaccgc ttctgccaga
      301 tgatattccc tgatctgtcc ggaattatcc agatccgcaa ttgtcctggc tacccgcaga
      361 attctgtgat aggctcttgt gcttagatca aactttttaa aagcatcttc caaaagcttc
      421 tctccctgcg ccgtaagctt acagtatctg tctatctcct tgattcccat tcctgaattg
      481 aacctgacgc agcggctttc ttcaaaaaac cgttcatatt gcctttgctg tgcctgtatg
      541 acccgccctc tgactgctgc cgaacattcc tcatctttct tttgcacaag ctcccgatat
      601 tttgcctgcc gtacctgtgt acaaatatcc atgcgatcca ggagcggctt ggagagcctg
      661 cttaaatacc tctttcttgc cgtttccgta caggtgcact tccctagatc cggataggcc
      721 ccgcatggac atggattcat ggctccgatc agcataaaat ccgccgggta tgtatagctt
      781 cctttgcttc tcgttacatg aacgacccgt tcttcaagcg gctggcgcaa aacctccaat
      841 gcacttcttg aaaactccgg aagctcatcc aaaaacagaa ccccatgatg cgccagtgaa
      901 atactgcccg gcctcggata gatcccccct cctgacagcg cagtggccgt caccgaatga
      961 tgaggactca caaacggcct ctccgtaatc agggcccgca tgccctgcag cttgcccgca
     1021 acactgtaaa cagcggatac ttccagacac tcttccatgg aaagcggcgg caggatcgac
     1081 ggaatacatt ttgcaagcat tgtctttcct gcccccggag atccgatcat gatcatatta
     1141 tgtaagccgg cagcggcaat ttccaatccc cgctttacaa aatgctgccc ggatacttca
     1201 ctaaaatcat aaccggcatt ctgtatcccg gacaacagtt tctcacctgc aaactgcttc
     1261 ggccgtattt tagaagtccc ttgtaaatga gcgaccagct gtatcagact ttccacgcag
     1321 atcacttcaa gtccgtcaac gaccgctccc tcggcttcat tctctttcgg gataatacag
     1381 cgccgcagtc ctgctttttt agctgcaagc atcagcggca gagctccgca gatcccgccg
     1441 accctgccgc ttaaaagcaa ttctcccacg atcatcgtat cttttatggc gtctgcagac
     1501 agctctccca ttgccactaa aataccgacc gcaacaggca ggtcaaaacc agttccctgt
     1561 tttctgacat tggcagggga cagatttact gtcacccgct ttaccggtat gagaaaccct
     1621 gcgtttttca gcgccgtccg aacgcgttcc cttacttccc tgacttcgct c
//
LOCUS       DAOSYE010000558         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63286_length_1668_cov_2.644761, whole genome shotgun sequence.
ACCESSION   DAOSYE010000558 DAOSYE010000000
VERSION     DAOSYE010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63286_length_1668_cov_2.644761"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLU43_09200"
     CDS             complement(<1..1012)
                     /locus_tag="PLU43_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO28629.1"
                     /translation="MDRELAKTYDPHGLEDRLYQKWMEKKYFHAKADRSRKPFTIVLP
                     PPNITGKLHMGHAFDSTMQDILIRFKRMQGYNALWVPGTDHAAIATETKVTAALKEQG
                     IDKAALGREGFLKRTWEWREEYGHAIVEQLKKMGASCDWDRERFTMDEGCSAAVEEVF
                     IDLYKKGYIYKGNRIVNWCPVCKTTISDAEVEHEEQAGHFWHIKYPIVGTDDYVVVAT
                     TRPETMLGDTALAVNPEDERYRELVGKTVLLPLVNKEIPVISDMYVDKEFGTGVVKIT
                     PAHDPNDFEVGRRHDLAVINIMNDDATINEQGGKYAGLGRYEARKQVVADLEALGLLE
                     KIE"
     gene            complement(1359..>1668)
                     /locus_tag="PLU43_09205"
     CDS             complement(1359..>1668)
                     /locus_tag="PLU43_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOO28630.1"
                     /translation="VTKSGRTARSISKYRPLSPIVAATYAEKTFHQLSLSWGVTPVLA
                     LFQNTATDLIRHGIDCAKQLDIVQNGDTVVVTAGVPVDTQGNTNLIKIEIVGSSAVGI
                     "
BASE COUNT          377 a          500 c          335 g          456 t
ORIGIN      
        1 cttcgatctt ttcaagcagt ccgagtgcct caagatcggc aacgacctgt tttctggctt
       61 cataacggcc aagtcccgca tacttcccgc cctgctcatt gatcgtggca tcgtcattca
      121 taatattgat gaccgcaaga tcatggcgtc tgcccacttc aaagtcattc gggtcatgtg
      181 ccggcgtgat ctttacaaca cccgtaccga attccttgtc tacatacata tcggaaatga
      241 ccggtatttc cttgttgaca agcggaagca gcacggtttt cccaacaagc tcccgatagc
      301 gctcatcctc aggattcacg gccagcgccg tatcaccaag cattgtttcc ggacgcgttg
      361 tcgcaacaac aacatagtcg tctgttccga cgatcgggta tttgatatgc cagaaatgac
      421 cggcctgttc ttcatgctca acctcagcat ccgaaatcgt cgttttacat acaggacacc
      481 agttgacgat ccggttgccc ttataaatat aacctttctt atacagatca atgaaaacct
      541 cttcgaccgc agccgaacag ccctcatcca ttgtaaaccg ttctctgtcc cagtcacagg
      601 aagcacccat ttttttcagc tgctccacga tcgcatgacc gtattcctct ctccattccc
      661 acgtgcgttt cagaaagcct tccctgccaa gcgccgcctt atcgatcccc tgctctttca
      721 gcgcagccgt gaccttggtt tccgtagcga tcgcagcatg gtcggttccc ggcacccaga
      781 gcgcattata tccctgcatt ctcttgaacc tgatcagaat atcctgcatc gtgctgtcaa
      841 atgcatggcc catatgcagc tttcccgtaa tattcggggg cggcaacacg atcgtaaacg
      901 gtttcctgct cctgtccgct ttcgcatgga aatacttctt ctccatccac ttctgataaa
      961 gccggtcctc cagtccatgc ggatcatatg tttttgccaa ttccctgtcc atccttaaaa
     1021 cctccattgt tttattctgc atatactatg aaacgtgcgc ccgcctgaca tgcaataaaa
     1081 agcccctgcc gtatacatac gacaagggcg actgctcgcg gtaccacctt gatttattgc
     1141 ttggctcctt aacgcggata aaacggatat ccctacttgc actgcagttc tcaggatacc
     1201 ggttcaaaag ctaccttccg catcctttct ccggacggcc tttcagccgg tgaaccgccc
     1261 tctctgacga gtgaacatgc gtactcctct ttctcataac cattacatca atttttttaa
     1321 tcataataaa gaatacctgg tttgtcaaat actttttgtc agatgccgac cgcgctgctg
     1381 ccgacgatct caatcttgat cagattcgta tttccctgtg tatcaacagg cacgccggcc
     1441 gttactacaa cagtatctcc gttttgcaca atgtccagct gttttgcaca gtcgatccca
     1501 tgacggatca ggtccgttgc cgtgttctga aacagagcga gcaccggcgt aactccccat
     1561 gacagggaaa gctgatgaaa cgtcttttcc gcatacgtcg ccgctacgat cggagacagc
     1621 ggacggtatt tggaaatgct tctggccgtt cttccgctct tcgtaacg
//
LOCUS       DAOSYE010000559         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63417_length_1666_cov_2.705773, whole genome shotgun sequence.
ACCESSION   DAOSYE010000559 DAOSYE010000000
VERSION     DAOSYE010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63417_length_1666_cov_2.705773"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLU43_09210"
     CDS             complement(<1..405)
                     /locus_tag="PLU43_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO28631.1"
                     /translation="MGLEEYKGVYVFAQQVDNEIGTIAFELLGKGKELAKDLDTDVTA
                     VLIGSGIGNLADKLAEYGADKVIVVDDPELKEYRTEPYAHALASVINKYKPEIVLVGA
                     TAIGRDLGPTVSARVATGLTADCTVLNIGDFPL"
     gene            complement(424..1209)
                     /locus_tag="PLU43_09215"
     CDS             complement(424..1209)
                     /locus_tag="PLU43_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO28632.1"
                     /translation="MKIIVCIKQVPDTKGGVKFNPDGTLDRGAMLAIMNPDDKAGLEA
                     ALRLKDQYGAEVTVLTMGLPKASDVLREAIAMGADKGILVTDRVLGGADTWATSTTIA
                     GAIRNLEYDLIITGRQAIDGDTAQVGPQIAEHLGIPVISYAQDIKIDKDKVIVQRQYD
                     DRYHILEAQMPCLITALSELNEPRYMTPGGIFDACKMEMTTWGRADLKDVEDCNLGLA
                     GSPTKIAKASDKVRKGAGERVIPESPEDAVSYIVGKLTEKHVI"
     gene            complement(1262..>1666)
                     /locus_tag="PLU43_09220"
     CDS             complement(1262..>1666)
                     /locus_tag="PLU43_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO28633.1"
                     /translation="LGLAEGALETTIAYVKERKQFGRSIAQFQNTQFQLADMATKVEA
                     AKLLVYKAAKKKDEYQTNPKTTYSFEAAQAKLYAAEVAMEVTTKAVQLHGGYGYIREY
                     DVERMMRDAKITEIYEGTSEVQRMVISANLLK"
BASE COUNT          390 a          455 c          326 g          495 t
ORIGIN      
        1 gagcggaaaa tcgccgatat tcagtacggt acagtcggct gtcaggcctg ttgctactct
       61 tgccgatacg gtaggtccca gatcccggcc gattgccgtt gcgccaacca gcacgatctc
      121 cggtttatat ttattgatta cggatgcaag cgcatgcgca taaggctctg ttctatattc
      181 cttcagctcc ggatcgtcta cgacgatcac cttatccgcg ccatactccg caagcttatc
      241 cgcaagattc ccgatacctg aaccgatcaa aacagccgta acatccgtat caagatcttt
      301 ggcaagctct tttcctttac caagtaattc aaatgcaatt gtgccgatct cattatcaac
      361 ctgctgggca aatacatata cgcccttata ttcttctaaa cccatcttcg ttcaccacct
      421 ttgttaaatt acgtgctttt cggttaattt gcctacgata tagctgactg cgtcttcggg
      481 agactccgga ataactctct ctcccgcacc cttacggacc ttatcggatg ctttggcgat
      541 ctttgtagga gaaccggcaa gacctaaatt acaatcttca acgtctttca aatctgctct
      601 gccccatgtt gtcatttcca tcttgcaggc atcaaaaatt ccgcccggcg tcatataccg
      661 aggctcgttt aattctgaaa gtgcagtgat cagacaaggc atctgcgctt caaggatatg
      721 atatctgtca tcatactgcc tctgtacgat gactttatct ttgtcgatct taatatcctg
      781 cgcataggaa attacaggaa ttccaagatg ctcggcgatc tgaggaccga cctgtgcggt
      841 atctccgtca atagcctggc ggcccgtaat gatcaggtca tattccagat ttctgatcgc
      901 tccggcaatc gttgtagaag tcgcccatgt atctgcacct ccaagtactc tgtctgttac
      961 gagaatacct ttatcagctc ccatggcaat tgcttcacga agtacgtcag atgccttcgg
     1021 aagacccatt gtaagcacgg ttacttccgc gccgtactga tcctttaatc taagcgctgc
     1081 ttccagaccg gccttatcat ccggattcat gattgcaagc attgctcctc tatcaagtgt
     1141 tccgtccgga ttaaacttaa cgccgccttt tgtatccgga acctgtttta tacaaacaat
     1201 gattttcacg attcttctct cctttttcat tttgtattgt aagccgacat caaatcatgg
     1261 cctacttaag cagatttgca gaaataacca tccgctgcac ttctgatgtt ccttcgtaga
     1321 tctccgtgat ctttgcatca cgcatcatgc gctctacgtc atattccctg atatagccat
     1381 aaccgccgtg aagctgaaca gcctttgtcg taacttccat ggcaacttcc gcagcataaa
     1441 gctttgcctg tgccgcttca aaggagtaag tagttttcgg atttgtctga tactcatcct
     1501 tcttttttgc agccttataa acaagcagct ttgcagcctc gacctttgta gccatatcag
     1561 ccagctggaa ctgtgtattc tggaactgag caatggaacg gccgaactgc tttctctctt
     1621 ttacgtaagc gatcgttgtt tccagcgcac cttccgcaag tccgag
//
LOCUS       DAOSYE010000560         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63462_length_1665_cov_3.486335, whole genome shotgun sequence.
ACCESSION   DAOSYE010000560 DAOSYE010000000
VERSION     DAOSYE010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63462_length_1665_cov_3.486335"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLU43_09225"
     CDS             complement(<1..1258)
                     /locus_tag="PLU43_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOO28634.1"
                     /translation="MRISVRNLVEFILRSGNIDNRHKGMQDASAMQEGSKIHRMIQRR
                     MGMDYHAEYFLRYIYPKQDYDIVIEGRADGIIFNEKESDQPVIVDEIKSTFSDMEYLK
                     KPVEVHLAQAKCYAYIFAEQKKLREIGVRMTYCNVDTQEIKYFHETCLFVEIRNWFME
                     ILDSYARWADFEFHWKKIRDVSIKNLQFPFPYRTGQKELAAQVYRTIYHQKRLFIEAP
                     TGSGKTLSTVFPSVKAMGEGRGDQIFYLTAKTITRTAAKDCFDILRGNGLQMKTVIIT
                     AKEKICPLGKPECNPEACPYAKGHYDRVNDAIYDLLMQEDSFSRDKIEEYAKKHTVCP
                     FEMGLDMSLFADAVICDYNYVFDPNVYLRRFFGEGAQGEYLFLIDEAHNLVERAMDMY
                     SATLYKESFLELKHLVEVSDKKLGKGL"
     gene            complement(1498..>1665)
                     /locus_tag="PLU43_09230"
     CDS             complement(1498..>1665)
                     /locus_tag="PLU43_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO28635.1"
                     /translation="ASKCDFDIDIFYNRVIIDAKSILGVLGLDFSQPLTVQYGGENCD
                     FENMIQRHVVG"
BASE COUNT          450 a          439 c          295 g          481 t
ORIGIN      
        1 ccaatccctt gccaagcttt ttatcagata cttcaaccag atgcttcagt tccaaaaaag
       61 actctttata taacgtggca ctgtacatgt ccattgcccg ctcaacaaga ttatgcgctt
      121 catctattaa aaacagatat tctccctgtg caccctcacc aaaaaaacgg cgcagatata
      181 catttgggtc aaacacataa ttataatcac agatgactgc atccgcaaat aaagacatgt
      241 ccagtcccat ttcaaatgga cagaccgtat gttttttcgc atattcttcg atcttatccc
      301 tggaaaaaga atcttcctgc atcagaaggt cgtagatcgc atcattgact ctatcataat
      361 gtcctttcgc ataaggacat gcttccggat tgcactccgg ttttccgagc ggacagatct
      421 tctcttttgc agtaatgatg accgttttca tctgaaggcc gtttccgcgc aggatatcaa
      481 aacagtcctt tgccgcagtt cgtgtgatcg ttttggccgt caggtaaaaa atctgatccc
      541 cgcgtccctc tcccattgcc tttacagacg gaaacaccgt cgaaagcgtc tttcctgaac
      601 cggtcggtgc ttcaatgaac agccgcttct gatgatagat cgtccggtat acctgcgcgg
      661 ccagctcttt ctgaccggtc ctgtagggaa acggaaactg taagtttttt atagatacat
      721 cgcgtatttt tttccaatgg aactcaaagt ccgcccacct tgcataactg tccagtatct
      781 ccatgaacca gttcctgatt tccacaaaca gacaggtctc atgaaaatat ttgatttcct
      841 gcgtatcaac attgcagtat gtcattctga caccgatttc ccggagcttc ttctgctccg
      901 caaagatata cgcataacac ttcgcctggg caagatgcac ttcaaccggt tttttcaaat
      961 attccatgtc ggaaaacgtg ctcttgatct catcaacgat caccggctga tccgattctt
     1021 tctcattaaa aataatcccg tccgcacgtc cttcgatcac gatatcataa tcctgtttcg
     1081 gataaatata acgcaaaaaa tattccgcat gataatccat ccccatacgc ctctgaatca
     1141 tccggtggat ctttgaacct tcctgcatgg cagacgcatc ctgcattcct ttgtgccggt
     1201 tgtcgatatt accggaacgg agaatgaact ctaccagatt ccgtacagag atccgtatac
     1261 tcggacgctc ctggccctct tcagtatgac cggcggcctg ccgttccctt ttttccgtat
     1321 tctttttagc ctgagccgta ttgtcaggta acaacggctt ttcataagtt tccgacaatg
     1381 tctgcccgtc cttccccatt cattaagaat catacccagt atagcaaaaa actctccgca
     1441 tggacagata aatccgtccc tacagagagt ctttgtttac ggttggaaaa ctgaaattca
     1501 gccaactaca tgtctttgaa tcatattctc aaaatcacag ttttctccgc catattgaac
     1561 agtcaatggc tgactaaaat ccaaaccgag cacgcctaaa atggattttg catcaatgat
     1621 tacacgattg taaaatatat caatgtcaaa atcacatttg gaagc
//
LOCUS       DAOSYE010000561         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63464_length_1665_cov_3.390062, whole genome shotgun sequence.
ACCESSION   DAOSYE010000561 DAOSYE010000000
VERSION     DAOSYE010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63464_length_1665_cov_3.390062"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLU43_09235"
     CDS             complement(<1..818)
                     /locus_tag="PLU43_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28636.1"
                     /translation="MSIVVSEVCKSFGERKVLDKLSFEIGKQNFTLLGPNGAGKTTLV
                     NMICGLLKYDSGKISVCGFDPKTEEQEVRKRIGLVTQDTSIYNYLTAKENLEFHANFY
                     GVPKNSVKSRVDEALALAQLEGRANDLVGTFSGGMKRRLALVRAMLHDPEIMILDEPT
                     LGVDVQNRNEIWNKILEMRGKKTIFINTNYMDEADSLSDICAVIDGGRVVAMDTPENL
                     KTGYSDGVKLTAEVKISKEEFPELRTKLKNYCADTKITEAAFENGYRISVPAKK"
     gene            complement(836..1453)
                     /locus_tag="PLU43_09240"
     CDS             complement(836..1453)
                     /locus_tag="PLU43_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO28637.1"
                     /translation="MKEKQVERDILNAAVKVFVEKGREGARMQEIADCAGVNKMLLHY
                     YFRDKDTLFLEVVREVNADLYGSVIEKSMEALTFQEFLSVFISRHFDYLNEKKDSFQF
                     LLWELSRKQFDLKTVVSGLNNKYGKNPFEILSDKLEEAVKNGEIREVDPTEFTLNLFS
                     LDLFMFIGLPVIREIMPMDEEALESALERREKEVFRLLWNDIKIK"
BASE COUNT          457 a          415 c          265 g          528 t
ORIGIN      
        1 ttctttttcg caggtacgga tatccgatat ccgttttcaa atgcggcttc cgtgatcttt
       61 gtatcagcac aataattttt cagttttgtg cgaagctccg gaaattcttc tttgctgatc
      121 tttacttccg ccgtaagctt tactccgtca gaatagccgg tctttaaatt ttccggcgta
      181 tccatggcca cgactctgcc tccgtcaatg accgcgcaga tatcggaaag actgtctgcc
      241 tcatccatat aattcgtatt gataaaaatc gtctttttcc ctctcatttc aagaatcttg
      301 ttccagatct cattcctgtt ctgaacatca acgccaagtg tcggctcatc caggatcatg
      361 atctccggat catgaagcat tgcccggacc agagcaagac gccttttcat tccgcccgaa
      421 aaggtcccaa caaggtcatt ggccctgcct tcaagctggg caagtgctag tgcttcgtcc
      481 accctgctct tcacactgtt ctttggcaca ccataaaaat tcgcatgaaa ttcaagattt
      541 tcttttgcgg tcagataatt ataaatggac gtatcctgtg tgacaagccc gatacgtttt
      601 ctgacttcct gctcctctgt cttcggatca aatccgcaga cactgatctt tccggaatca
      661 tatttcaaaa gtccgcagat catattgact aacgtcgttt ttcctgctcc gtttggtcca
      721 agcagcgtaa aattctgctt tccgatttca aaagaaagct tatccagaac ctttctttct
      781 ccaaaagatt tacatacttc tgatacaaca atggacatat ctgttccctc caactctatt
      841 tgatttttat atcgttccag agaagtctga agacttcttt ttccctgcgc tcaagcgcac
      901 tttccaatgc ctcttcatcc atgggcatga tctccctgat cacaggaagc ccaatgaaca
      961 taaacagatc caagctgaaa agatttaacg tgaattccgt tggatctact tcacggatct
     1021 ccccgttttt cacagcttcc tccagtttat cggaaagaat ttcaaaagga ttttttccat
     1081 atttgttatt taaaccggaa acgacggtct ttagatcgaa ctgcttcctg cttaattccc
     1141 acagtaaaaa ctgaaaggaa tccttttttt cattcaaata atcaaaatgc cggctgatga
     1201 acacggataa aaattcctga aatgtcaagg cttccatgct cttttcaata acactgccgt
     1261 acaaatctgc atttacttcc ctgacgacct caagaaacag cgtatccttg tctctaaaat
     1321 aatagtgcag cagcatttta ttaacacctg cacaatcggc aatttcctgc attctggcac
     1381 cttccctgcc tttttcaaca aaaaccttga cggccgcatt caaaatatcc cgttccacct
     1441 gtttttcttt catatttttc gcctcttttc aagaaatata tcatttggtt taaccatctg
     1501 gttaaataat agtatacaac tccatgcacg tcaactgatt ttaaaaaatc aaacgaaagt
     1561 tgacatacag ttgtcaactt tcgtttgata taataaaaca acatgaaaaa aaggagtatc
     1621 cattcctatg atcagaaact attttatgtc aaatggtttg aaact
//
LOCUS       DAOSYE010000562         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63726_length_1661_cov_3.996887, whole genome shotgun sequence.
ACCESSION   DAOSYE010000562 DAOSYE010000000
VERSION     DAOSYE010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63726_length_1661_cov_3.996887"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLU43_09245"
     CDS             <1..1136
                     /locus_tag="PLU43_09245"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HOO28638.1"
                     /translation="LFERMDQASETFKMLEVVRTINDGGIRAVCFAENGSEDATVVFR
                     GTGGEYEAWRDNLYGEYMAETDMQKLASDFIVYDCGIYENITVSGHSKGGNLAQYVTA
                     VCPDKIDRCVSYDGQGFGEKFFEKNGAAVSTAASKIISVSAHNDFVNILLTPIAGKRI
                     FVENSGTGAYAHSSYWMLVSSRFDENGSFLSITRQDMAMQVLERAVSFAAKRLDRLPA
                     DGNVVFGNLLASLTASVMSNEKSKEYEQNELRQAADALHEYALSFINISGRFQEEPIK
                     LITCYNYMAVVNMKHVCANLQEALEAVGRTAGKMEDMEGRMQDRLIFRLYTDIAVRRI
                     ISRLYDEQKKIIVLCEVLREVISLYEVKETQLAGQIALTVLQN"
     gene            complement(1117..>1442)
                     /locus_tag="PLU43_09250"
     CDS             complement(1117..>1442)
                     /locus_tag="PLU43_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HOO28639.1"
                     /translation="SPLMSRWESIKPYCFQLIKGKKTPLSFRFVFYLAPENTERFLKN
                     TATELTLNDINGLSLNIRYNGHTLSCTTATSLNLFTLDKSIEYAWDAMIKKFLTKNEI
                     NFEEQ"
     assembly_gap    1443..1452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1453..1643)
                     /locus_tag="PLU43_09255"
     CDS             complement(<1453..1643)
                     /locus_tag="PLU43_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HOO28640.1"
                     /translation="MLLLNITDVKAFMKKLLSENETTFDQFLLSEASVETGNTFTIDG
                     HINQKFYSAEETELLTLTA"
BASE COUNT          471 a          280 c          445 g          455 t
ORIGIN      
        1 tattatttga aaggatggat caggcatccg agacctttaa aatgctggag gtcgtcagaa
       61 cgatcaatga cggaggcatc agggcagtat gttttgcgga aaacggaagt gaagacgcaa
      121 cggtcgtatt tcgagggaca ggaggagaat atgaggcatg gagagacaac ctgtatggag
      181 agtatatggc tgaaacggat atgcagaaac tggcttcgga ttttattgtg tatgactgcg
      241 gaatatacga aaatattacg gtcagcgggc attccaaagg cggtaatctg gcccagtatg
      301 ttacggctgt ctgtccggat aaaattgatc gctgtgtctc ttatgacgga cagggctttg
      361 gagaaaaatt ttttgaaaaa aacggggctg ccgtttcaac ggcagcttcg aaaataatct
      421 ctgtcagcgc gcataatgat tttgtaaata ttttgctcac accaattgca ggaaaacgta
      481 tatttgtaga aaatagcggg acaggagctt atgcgcacag cagttactgg atgctggtaa
      541 gcagccgttt tgatgaaaac ggcagttttt tgagcattac acggcaggat atggcgatgc
      601 aggtgctgga gagagcggtt tcttttgctg caaaaagact ggatcgtctg ccggcagacg
      661 ggaatgtagt atttggaaat cttcttgcat cgcttacggc ttctgttatg agcaatgaaa
      721 aaagcaagga atacgagcag aatgaattga ggcaggcagc agatgccctg catgagtatg
      781 ccctgtcctt tataaatata tccggaagat ttcaggagga gccgataaag cttattacat
      841 gctataatta tatggctgtg gtcaatatga agcatgtgtg tgcaaatttg caggaggctt
      901 tagaagcggt cggccggact gcgggaaaaa tggaggatat ggaggggcgg atgcaggatc
      961 gcctgatttt caggctttat acggatattg ccgttcgaag gatcatcagc agactttatg
     1021 atgagcaaaa aaaaataatt gtattatgcg aggtgcttag ggaagtcatt tccctttacg
     1081 aagtgaaaga aacacagctg gccggacaga tagcgcttac tgttcttcaa aattgatttc
     1141 gttttttgtc aggaattttt tgatcatggc atcccatgcg tattctatgg atttatcaag
     1201 tgtaaacagg ttcagcgatg ttgcggtcgt acaggaaagt gtatgtccgt tatatctgat
     1261 atttaaagag agtccgttga tatcgttaag agtcagttcg gtagccgtat ttttcaggaa
     1321 acgctctgta ttttccggag caagatagaa aacaaaccga aaggacagcg gagttttctt
     1381 gcctttaata agctgaaaac aatagggttt tattgattcc cagcgtgaca tgagagggga
     1441 gtnnnnnnnn nnttggcggt cagagttaac agctcggtct cttccgcgga gtagaacttt
     1501 tggttgatat gtccgtcgat cgtaaaggta ttgccggttt caacagaagc ttccgacagc
     1561 agaaactgat caaatgtggt ctcgttttca gaaagcagtt ttttcataaa ggctttgaca
     1621 tccgtaatgt tgagcagtaa cataaagtcc tccgtgatgt g
//
LOCUS       DAOSYE010000563         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_63869_length_1659_cov_3.211970, whole genome shotgun sequence.
ACCESSION   DAOSYE010000563 DAOSYE010000000
VERSION     DAOSYE010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63869_length_1659_cov_3.211970"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PLU43_09260"
     CDS             <1..1285
                     /locus_tag="PLU43_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28641.1"
                     /translation="AIVLTGYSEFEYAQRAIQLEVVEYLLKPLNIEDIIEVLNTVSKK
                     LSKNKIETASAEQLLFSVLTGDESEQELVQRQFDEKIRHQPEQPISMFLIRSESILEE
                     TTNQMVDVLQDTLNTICLTGYYIFKLPFEKEILVMILDGQNVHYLKEIFKMRVTRELK
                     KTGEYLICYDEISNISMLKEKLRQMQDYFMYTFVCPENCILDTEVIKNLTFKKVDYPE
                     YLENAIRRDIRNGNKEGIRKTAGKFEETVIHSREEPVTIKKYTVRFVVNILDTARDLL
                     ENKDIESLYNYLLNDMMHSNIKELFLNNYWKMIDMIAGEKKEDALTENGMILNVIEFI
                     RQNYDKEISLSEAAELVGITPEYLSKLFSKEMGINFSTFLGEFRVSIAKKLLVTGNYK
                     IYEVAEMVGYKDTKYFNKVFRSIMGVSPSDYRKVL"
     gene            1288..>1659
                     /locus_tag="PLU43_09265"
     CDS             1288..>1659
                     /locus_tag="PLU43_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28642.1"
                     /translation="MNRITQRILAVLWAVVFLLLISVIIHYYAETKTETVIEPVREEL
                     VIMVTYEEESNYEYLKEVVQAYSETEGNPEVTLQYVTQSGFQKQLCIDKDQNKLPDLI
                     ICENVMTPALQSMGILEELSAY"
BASE COUNT          567 a          258 c          402 g          432 t
ORIGIN      
        1 ggcgatcgta ctgaccggat attcggaatt tgaatatgca cagagggcga tccagctgga
       61 agtagtggaa tacctgctga agccgctgaa tattgaagac attatagaag tattgaatac
      121 ggtgtcaaaa aagctgtcca aaaataaaat tgagactgca tccgcagaac agctgctgtt
      181 ttccgtgctg acaggagatg aaagcgaaca ggagcttgta cagcggcagt ttgatgaaaa
      241 aataaggcac cagccggagc agcccatatc catgtttttg atccggtcgg aaagtatact
      301 ggaggaaaca acgaaccaga tggtcgatgt tcttcaggat acgttaaata cgatctgcct
      361 gacagggtat tatattttta aactgccctt tgaaaaagaa attcttgtta tgatcttaga
      421 cgggcagaat gtgcattatc tgaaagagat ctttaagatg agagtgaccc gtgaattaaa
      481 aaaaacaggg gaatatttga tctgctatga tgagatatca aatatttcca tgctgaaaga
      541 aaagcttagg cagatgcagg actattttat gtatacattt gtatgtccgg aaaactgcat
      601 tcttgatacg gaggtgataa aaaatcttac atttaaaaag gttgattatc cggaatatct
      661 ggaaaatgcg attcgcaggg atatcagaaa cggaaacaaa gaagggatcc ggaaaacggc
      721 cgggaaattt gaagaaacag tgattcacag cagggaggaa ccggtaacga tcaaaaagta
      781 tacggtacgc tttgtcgtaa atattttaga tacggccagg gatctgcttg aaaataaaga
      841 tatagagtca ttatacaatt atctgctgaa tgatatgatg cattctaaca taaaggaatt
      901 gtttttgaat aattactgga aaatgataga catgatcgca ggagaaaaaa aggaagacgc
      961 attaacggaa aacggtatga tcctgaatgt cattgaattt atccggcaga attatgataa
     1021 ggaaatatca ctttctgagg cagctgaact ggtcggaata acaccggaat atctgagtaa
     1081 actgttttca aaagaaatgg gtattaattt cagcacattt ttaggagagt ttcgtgtgag
     1141 cattgctaaa aaactgcttg taaccggaaa ctataaaata tatgaagtgg cagagatggt
     1201 agggtataag gacaccaaat atttcaataa ggtttttcgc tctattatgg gggtatcccc
     1261 gtcagattac agaaaggtat tgtaacaatg aacagaataa cacaaagaat acttgccgta
     1321 ttgtgggcag ttgtgttttt gctgcttatc agcgtcatta ttcattatta tgcagaaacg
     1381 aaaacagaaa cggtcataga gcctgtgcgg gaagagcttg tgattatggt gacatatgag
     1441 gaagagagca actatgaata tcttaaggag gtcgtgcagg catattcgga aacagaaggg
     1501 aacccggaag tgacgctgca gtatgtcacg cagtcagggt ttcagaaaca gctttgtatc
     1561 gacaaggatc agaataagct gccggatctg attatttgcg aaaatgtgat gacaccggct
     1621 ctgcagtcaa tgggaatact ggaagaactg tctgcttat
//
LOCUS       DAOSYE010000564         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_64202_length_1654_cov_2.807380, whole genome shotgun sequence.
ACCESSION   DAOSYE010000564 DAOSYE010000000
VERSION     DAOSYE010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64202_length_1654_cov_2.807380"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLU43_09270"
     CDS             <1..888
                     /locus_tag="PLU43_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28643.1"
                     /translation="PGDPFLYLSVEDGDVTVAYTEEQIELYKAYYGLDEPLYIQFFNY
                     ITGIFKGDLGYSIYYKTSVVKMIAARIPWTVFIVLCSLFFSSVIGTVLGALSARLREK
                     PADRLVYFFMIVFSEIPSFLLGTLLLIFFAAKLKWFPLSGGSTAFAVYPSTLAYLADL
                     LYHAALPILTLTLAGLGGFYLLSRNSMLTVMTKDYIRTAKAKGLSKMRILFRHALKNA
                     LPPVIARIFMSMGAMFGGAVLIENVFAYPGLGQLMREAVLARDYVLTQGIFLVIALMV
                     LVMNWAADMVYKKLDPRVV"
     gene            895..>1654
                     /locus_tag="PLU43_09275"
     CDS             895..>1654
                     /locus_tag="PLU43_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28644.1"
                     /translation="MQTIFKSKNFSAYGKAGLIVIFIIIVMGLASVLFFGDAHRVLTG
                     SSLEAPSFEHLLGTDDLGMDIFAQICHGALISLTVGFCSAALAGIGGSILGILAGYAG
                     KWADRLICGLCDIMAVIPQLPLMIVLGAFFGPSIRNIIFVIAILSWVGPARIARSKVL
                     SLRNEKYIAAAKSYGASFFHILTKHLLPGLLPVIMVSTIRIVSHAVIAEAGLTFLGLG
                     DPTSKSWGVILNRAMSVGGIYFTEFWKWWILPPLT"
BASE COUNT          355 a          320 c          449 g          530 t
ORIGIN      
        1 cccggcgatc cgttcctgta tctgtccgta gaagacgggg atgttaccgt tgcgtatacg
       61 gaagagcaga tcgagctgta taaagcgtat tatgggcttg acgagccatt gtatatacag
      121 ttttttaatt atatcacggg tatttttaaa ggtgatctcg gatatagtat ttattacaaa
      181 acaagcgtcg ttaaaatgat tgcagcccgt attccatgga cggtttttat tgtgctgtgt
      241 tcgctgtttt tcagcagtgt gatcggcaca gtactgggag cgttatctgc ccggcttcgt
      301 gagaagccgg cggataggct cgtttatttt tttatgattg tgttttcgga gataccgtct
      361 tttctgcttg ggacactgct gctgattttt tttgccgcaa aactgaaatg gtttccgctt
      421 tccggcggat ctacggcatt tgcagtctat ccgtcaacat tggcatatct tgcggatctg
      481 ctgtatcatg cggcgctgcc gatattgacg cttacgcttg caggacttgg cggattttat
      541 cttctctcaa gaaacagtat gcttacggtg atgacaaaag actacatccg tacggcaaaa
      601 gcaaaaggat tgtctaaaat gcgcattttg ttccggcatg cgcttaagaa tgctctgccg
      661 ccggttattg cgcggatatt tatgagcatg ggagcgatgt ttggaggagc cgtgcttatt
      721 gagaatgtat ttgcctatcc gggattggga cagctgatga gggaagctgt gctggcccgt
      781 gattatgttc tgacacaggg aatttttctt gttattgcgc ttatggtgct tgtgatgaac
      841 tgggcggcag atatggttta taaaaaactt gatccaaggg tggtgtaaat agagttgcag
      901 acgatcttta aatctaaaaa tttttcagca tatggaaagg cagggctgat tgttattttt
      961 ataatcattg tcatgggtct tgcttctgtt ctgttttttg gagatgccca tcgggttttg
     1021 accggttctt ctttggaagc tcccagtttt gagcatcttc ttggaaccga tgatcttggg
     1081 atggatattt ttgcgcagat ctgccatggt gccctgatca gtcttacggt tggtttctgt
     1141 tcggcggcgc tggccggaat cggcggcagt atccttggaa tactggccgg ctatgcaggc
     1201 aagtgggcgg accggctgat ctgcggactg tgtgatatta tggcagtcat tccgcagctt
     1261 ccgctgatga tcgtgcttgg cgcttttttt ggtccgagca tcaggaatat tatttttgtg
     1321 attgcaattc tgtcatgggt cggaccggcc agaatcgcgc ggtcaaaagt attgtccctg
     1381 cgaaacgaaa aatatatagc cgcggcaaaa agctatggcg cttctttttt tcatattttg
     1441 acaaagcatt tactgccggg gcttcttccg gtaattatgg tcagtacgat acggatcgtc
     1501 agccatgctg taattgctga ggcaggactg acttttttgg gacttggtga tccgacatcc
     1561 aaaagctggg gtgtgatatt gaatcgtgcc atgagcgttg ggggaattta ttttacggaa
     1621 ttttggaagt ggtggatcct tccgccgctg actg
//
LOCUS       DAOSYE010000565         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_64304_length_1652_cov_4.060739, whole genome shotgun sequence.
ACCESSION   DAOSYE010000565 DAOSYE010000000
VERSION     DAOSYE010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64304_length_1652_cov_4.060739"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLU43_09280"
     CDS             complement(<1..257)
                     /locus_tag="PLU43_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28645.1"
                     /translation="MNSTEEYLDNLLKSVTEGKEISAEQNSEIVSETPVMPEPERENV
                     SEMEDALEPSDESVNEPLESPEEVFELPESTEEVPVNDNDV"
     gene            complement(357..986)
                     /locus_tag="PLU43_09285"
     CDS             complement(357..986)
                     /locus_tag="PLU43_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28646.1"
                     /translation="MDDLKKQEVLRAITEADMVLVGIGKELEAADDHDSKQIADVCRT
                     YEKLAVLLKEKNYFIITTNYDDKIYDSSINKDRIVAPCGSVHRFQCAKGCTDALYMEK
                     RGSCPVCGALLCENTVKAENYIESGYLPQWNKYIKWLSGTLHKKLCVLELGVLMEFPS
                     VIRWPFEKTVFLNETAFLFRINEQLPQLSVELKEKGMSIACHPILFFAE"
     gene            complement(1053..>1652)
                     /locus_tag="PLU43_09290"
     CDS             complement(1053..>1652)
                     /locus_tag="PLU43_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28647.1"
                     /translation="KVIGIHRVSWTDIIKPDVTINNDQDGISTGIVKFDDRLVIILDF
                     EKIVTDISPETGLKLTEIAELTERERNSKAVLIAEDSHLLNKLISDALLKAGYINIKK
                     TENGLEAWNYINQCLEEGTVNEKIKCVITDIEMPQMDGHHLTKLIKSDERLKQIPVII
                     FSSLVNEEMRVKGEALGADAQLSKPEIGNLVKEIDKLLQ"
BASE COUNT          475 a          385 c          235 g          557 t
ORIGIN      
        1 aaaacatcat tatcattaac cggaacctct tcagttgatt caggcaattc aaaaacttcc
       61 tcgggagatt ccagcggctc attgacagat tcatcacttg gttccaaagc atcttccatt
      121 tcggatacat tttcacgttc cggttctggc ataacaggag tttcagacac gatttcagaa
      181 ttttgttcgg cagatatctc ttttccctct gttacgcttt taagcaaatt gtccagatat
      241 tcttccgtag aattcataag gccctccgaa ataaaatctt taaaataatt accgaaacaa
      301 catatataat ataacagtct atcatatttt tcattgtaaa acaatgtatt tacaggtcat
      361 tcagcaaaga atagaattgg atgacatgca atcgacattc ctttttcctt taattcaacg
      421 gaaagctgag gcagctgttc attgattctg aaaagaaatg ctgtttcatt taaaaatacc
      481 gttttttcaa atggccatct gatcacggaa ggaaattcca tcaaaacccc taattctaaa
      541 acacatagtt ttttatgcag cgtccctgaa agccatttga tatatttgtt ccattgcggt
      601 agatatccac tttcaatata attttctgct ttcaccgtat tttcacataa caatgctccg
      661 cataccggac aacttcctct cttttccata tataaagcat ctgtacagcc ctttgcacat
      721 tgaaaccgat gaacgcttcc gcacggagca acgattctgt ctttattaat tgaagaatca
      781 tagattttat catcataatt agttgtaatt ataaaataat ttttttcttt taacaatacg
      841 gccagttttt catacgtacg gcagacatct gcaatttgtt tactgtcatg atcatccgca
      901 gcttccagct cttttccaat accgaccaag accatgtccg cttccgtaat cgctcgaagt
      961 acttcctgct tttttaaatc gtccatttca catatccttt acatcataat aggaccgagt
     1021 tatgacccgg ccctactgca cttttcaccg tattattgca acagcttatc aatttccttt
     1081 acaagatttc caatctctgg ctttgagagc tgagcatccg caccaagcgc ctcacctttc
     1141 acacgcattt cctcatttac gagtgatgaa aaaataataa ccggtatctg tttcagccgt
     1201 tcatcgctct ttataagctt tgttaaatga tgtccatcca tctgaggcat ttctatatcc
     1261 gtaataacac acttgatctt ttcgttgact gtcccttctt caagacactg attaatataa
     1321 ttccaggctt ccaaaccgtt ttctgtcttc tttatgttaa tataaccagc ctttaacagc
     1381 gcgtcactga tcaatttatt caaaaggtgg gaatcctccg ctatcaaaac cgcttttgag
     1441 tttctttctc tttcagtaag ctccgcaatc tccgtcagct ttaatcctgt ttccggactg
     1501 atatcggtta cgatcttttc aaaatcaaga ataataacca ggcgatcatc aaattttaca
     1561 ataccagttg aaattccatc ctgatcatta ttaattgtga catccggctt aataatgtct
     1621 gtccatgaaa ctctatggat accaattacc tt
//
LOCUS       DAOSYE010000566         1651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_64407_length_1651_cov_2.137845, whole genome shotgun sequence.
ACCESSION   DAOSYE010000566 DAOSYE010000000
VERSION     DAOSYE010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64407_length_1651_cov_2.137845"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PLU43_09295"
     CDS             complement(<1..1304)
                     /locus_tag="PLU43_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28648.1"
                     /translation="MKAFIKTHSLLLLILTEAVLTILLIIFLLTRPLSDITVPITEGT
                     PEIGRIDADGYWFDMEDGEQVSSSDEKILFHLSAFVPKGSYTLIVQYASGAGSKCGLT
                     CTDTGAIQCDDISLNHATSVQTANVTVNKSVSDMDFYVKYCGFKTFHIESVTLTGNHK
                     NVIPALVKWVFLLCIMDLLFWLLIKKKISLLHNQSVAVILCLIGFTLIVSIPFYGSYL
                     PLMDDFAFSYIKIEGIRDGLLDGQLPVRIHPNTQLGYGYALPYFYPELFLYFPAVLRL
                     LGFSIMEAYKIFVFTVNFATAAIAFYSIQKIFKNHLIALSGSFLYTFSIYRLIDVYRR
                     ASIGEYLAMIFLPLIIAGLFLILSEDTKTPQYKRNSFPLLFGLTGLACSHILSCEMAG
                     IFILLALILCAKRTFQKERLLLLVKTALGTLFLTAGFWIPFF"
     gene            1428..>1651
                     /locus_tag="PLU43_09300"
     CDS             1428..>1651
                     /locus_tag="PLU43_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28649.1"
                     /translation="MRGRFVKKWFELTKKKQYVIISGIILFFFILIHIFKLDQIPYGY
                     HIDEAGAAYDSYCLSYFGIDRYLNEFPVYL"
BASE COUNT          549 a          297 c          365 g          440 t
ORIGIN      
        1 tcaaagaatg ggatccagaa gcctgcagtc agaaacaaag tgccaagagc tgttttaacc
       61 aaaaggagca gccgctcttt ttgaaatgtt cgtttggcgc acagtatcag ggcaagcagt
      121 ataaaaatgc ctgccatttc gcagcttaga atatgggagc aggcaagtcc ggtgagtccg
      181 aacagcaggg gaaacgaatt ccgcttatat tgcggcgttt ttgtatcctc agacaggatc
      241 aggaaaagac cggcaatgat cagcggcaga aagatcattg ccagatattc tccgatcgag
      301 gcacgccgat aaacatcgat taggcggtaa atggaaaaag tatacaaaaa actgccggac
      361 agcgcaatga gatgattttt gaatattttt tgaatactgt aaaaagcaat ggcagcagtt
      421 gcaaaattga ctgtaaaaac aaagatctta taagcctcca taatggaaaa tccgagcagg
      481 cgcaaaacag caggaaaata gagaaacagc tctggataaa aatatggcag tgcatagccg
      541 tagccaagct gtgtatttgg atggatcctg acgggaagct gtccgtccag cagaccgtcc
      601 ctgatccctt cgatcttaat atagctgaag gcaaaatcgt ccatcagcgg cagataggag
      661 ccgtagaacg gtatggaaac gataagtgta aatccgatca gacataaaat aactgccaca
      721 gactgattgt gaagaagaga aattttcttt tttataagca gccaaaacag cagatccatg
      781 atacaaagca ggaaaaccca tttcacgaga gccggaataa catttttatg attgccggtc
      841 agtgtgacgc tttcaatatg aaaggttttg aatccgcagt attttacata gaagtccata
      901 tcggatacgg atttatttac agtaacattt gccgtctgca cagaggtggc atgatttagc
      961 gaaatatcgt cacattggat ggcacctgtg tccgtacagg tcagcccgca tttacttccg
     1021 gcaccggaag catattgaac aatgagtgta taagaacctt tcggaacaaa agcagaaagg
     1081 tgaaacaata ttttttcatc ggaagaagat acctgttccc cgtcttccat gtcgaaccag
     1141 tatccgtccg catcgatccg gccgatttca ggcgttcctt ccgtaatggg aactgtaata
     1201 tccgaaaggg gtcttgtcag taaaaatata atcagcagga tagtcagaac tgcttccgta
     1261 agaataagga gcagcaggct gtgagttttt atgaaagctt tcaatgagat taccgccttt
     1321 acaaatattt ctacgattaa ttatagaagt aaatgaaact tttgacaagt aaatctgaat
     1381 ataatataat aaatagaatt tattggataa attccggagg attttaaatg aggggaagat
     1441 ttgtaaaaaa atggtttgaa ttgactaaaa aaaagcagta tgttattatc tccggaatta
     1501 tattattttt ttttatattg attcatattt ttaaactaga ccagattcca tatggatatc
     1561 atattgatga ggcgggggcg gcgtatgatt cgtattgtct ttcatatttt ggaatagaca
     1621 gatacttaaa tgaatttcca gtatatttaa t
//
LOCUS       DAOSYE010000567         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_64893_length_1643_cov_3.602645, whole genome shotgun sequence.
ACCESSION   DAOSYE010000567 DAOSYE010000000
VERSION     DAOSYE010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64893_length_1643_cov_3.602645"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="aroF"
                     /locus_tag="PLU43_09305"
     CDS             complement(<1..910)
                     /gene="aroF"
                     /locus_tag="PLU43_09305"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOO28650.1"
                     /translation="MIIVMKPSATKNNIEAVMALIKEKGLEPHLSEGSEVTIIGVIGD
                     KTRLCNDNLEIFEGVDKVVPLTESYKLTNKKFHPDPTTVKVGNTLIGPLSMTVMAGPC
                     AVENEEQLMLIAKEVKKSGATIVRGGAYKPRTSPYAFQGLAEEGLRYMQEAGKETGLA
                     TVCEVVSLDAVNAAVKYVDMLQIGARNMQNFILLKEAGRTGLPVLLKRGLSATIDEWL
                     NAAEYIIAEGNPNVVLCERGIRTFETSTRNTLDISAVPVLKSKTHLPVIVDPSHATGV
                     RAYVKPLAKAAIAAGADGLMIEVHNDP"
     gene            1223..>1643
                     /locus_tag="PLU43_09310"
     CDS             1223..>1643
                     /locus_tag="PLU43_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO28651.1"
                     /translation="MNVYTSDKIRNVVLLGHGGCGKTTLVEAMAYLSGITSRMGKTND
                     GNTISDYDKEEQKRQFSIGTSVVPIEWDSYKINLLDTPGYFDFVGEVEEAVSAAAAAV
                     IVISGKAGIEVGTEKAWELCEKYKLPRIIFVTDMDVDN"
BASE COUNT          468 a          319 c          372 g          484 t
ORIGIN      
        1 aaggatcatt gtgtacctcg atcattaaac cgtcggcacc ggcagcaatt gcggccttcg
       61 caagaggctt tacataggca cggacgcctg ttgcgtggga agggtcaacg atgacaggca
      121 ggtgtgtctt tgattttaat acgggaacag cactgatgtc cagtgtattt cttgtggaag
      181 tttcaaatgt acgaatgccg cgttcgcata gtacgacatt tggattgcct tccgcgataa
      241 tatattccgc tgcattgagc cattcatcga tcgttgcgga caatccgcgt ttcagaagaa
      301 cgggcagtcc ggttctgccg gcttctttca gcaatatgaa attctgcata ttacgggcac
      361 cgatctggag catatcgaca tatttaacgg cggcattgac cgcatccaga ctgacgactt
      421 cacagactgt ggcaagaccg gtttcctttc cggcttcctg catatagcgc agaccttctt
      481 cggcaagtcc ctggaaagca taaggagaag ttcttggctt ataggcgccg ccgcgtacga
      541 tcgttgcacc tgattttttg acctcttttg caatcagcat cagctgttcc tcattttcta
      601 ccgcacacgg gccggccatg accgtcatgc ttaaaggacc gatcaaagta tttccgactt
      661 tgacagtagt aggatcagga tgaaattttt tatttgtaag tttatagctt tcagtcaggg
      721 gaacaacttt atcgacacct tcaaatattt cgagattgtc attacagagc cgggtcttgt
      781 ctccgatcac accgataatc gttacttcgc tgccttctga gagatgcggt tccagtcctt
      841 tttctttgat cagggccata acggcttcga tattattctt tgtagcactg ggtttcatta
      901 caataatcat gatggtcaac ctttcttttc catatgtgat ataacatcaa tctgttctct
      961 catattttat gtcagcttat aagaattgtc aatactgcat cactttaaag cgcaacgctt
     1021 ttaagtggta aaatatacaa acaatatgga aaagaaaaga tgatttgata aggaaaaata
     1081 aagtatttgt gaaaattgta taatgctatt gcaaattatg aataagttta ttacaatatt
     1141 tacatgagtg caatcataat aaatacaaat tattgtattt atttacgaaa tctcaaaaca
     1201 acttgatatg gaggaataga atatgaatgt ttacacaagt gacaagattc gtaatgtagt
     1261 gcttttagga cacggaggct gcggtaagac aacgcttgtt gaagcaatgg catacctgtc
     1321 aggaattact tcgcgtatgg gcaagacaaa tgacggaaat acgatcagcg attatgataa
     1381 agaagaacag aaacgccagt tttcaatcgg aacaagtgtt gtgccgatcg aatgggattc
     1441 atataaaata aatttacttg atacgccggg ttattttgat tttgtcggag aagttgagga
     1501 agcggtcagt gcggcagcgg cagctgttat cgttatttcc ggcaaggccg gtattgaagt
     1561 cggcacggaa aaggcatggg aattatgcga aaagtataaa ttgccgagaa ttatttttgt
     1621 tacggatatg gatgtggaca atg
//
LOCUS       DAOSYE010000568         1642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_64987_length_1642_cov_3.158790, whole genome shotgun sequence.
ACCESSION   DAOSYE010000568 DAOSYE010000000
VERSION     DAOSYE010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64987_length_1642_cov_3.158790"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLU43_09315"
     CDS             complement(<1..1190)
                     /locus_tag="PLU43_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOO28652.1"
                     /translation="MKSKIELLSPAGDYECFTAAVNAGADAVYLGGNRFGARAYAQNF
                     SDEELKAAIDYAHLFGKKVYLTINTLFKNEELHELDFYLESFYYWGLDAVIVQDIGVI
                     SHIRRMFPELPVHASTQMAITDTEGVLFLKELGIKRTVLARELSLPEIEEIHHRTDME
                     LECFIHGALCYSYSGTCLFSSLIGGRSGNRGRCAQPCRLPYNGKYLLSTKDICTLELL
                     PQLLSAGITSFKIEGRMKAPDYVAGVTGIYRKYIDSYLHEPETYRVKEADLQTLLQLY
                     TRSGNSRGYYFQRNGETMISVESPCYKTADQAERKEVYDSYAGHEKKLPLQMTILLKK
                     GSRASLTVRCHTTELCVLGNLVEAAKKQPVSEDAIKKQLNKTGGTPYRIETLHIISDP
                     DVFIP"
     gene            complement(1268..1633)
                     /locus_tag="PLU43_09320"
     CDS             complement(1268..1633)
                     /locus_tag="PLU43_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOO28653.1"
                     /translation="MASKNDTEVIIGGKVITLCGYESEEYLQKVASYINNKIAEYSKV
                     DSFRRQPLDMQSVLLQLNVADDYFKAKKQIEALEEEIEAKDRELYDLKHELIASQIKL
                     ESKEKMIRSYQNEISDRKR"
BASE COUNT          421 a          402 c          299 g          520 t
ORIGIN      
        1 ggaataaaaa catcagggtc cgatataata tggagcgttt ctatccgata cggggtgccg
       61 ccggttttat tcagctgttt tttaattgcg tcttcagaaa ccggctgttt tttagccgct
      121 tccaccaggt tgcccaaaac acaaagttcc gttgtatgac accgtacggt cagagatgcc
      181 ctactgccct ttttcaacag aattgtcatc tgtaaaggaa gttttttttc atgaccggcg
      241 taactgtcat aaacttcttt ccgttctgcc tgatctgcag ttttatagca ggggctttct
      301 acggagatca tcgtttcgcc gtttcgctga aaataatacc cgcggctgtt gccgctcctt
      361 gtatacaact gcagcagagt ctgcagatct gcctctttca ccctgtatgt ttcaggctca
      421 tgcagataac tgtcgatata ctttcgataa ataccggtca caccggccac ataatctgga
      481 gctttcatcc ggccttcgat cttaaaggaa gtaatcccgg cgctgagcag ctgcggcagt
      541 aattccagcg tgcagatatc ctttgtactg agcagatatt ttccgttata gggaagacgg
      601 catggctgtg cacatcgtcc cctgtttccg cttctccctc cgatcaggct ggaaaaaaga
      661 catgttccgg aatagctgta acataaagca ccgtgtataa aacattccag ctccatatcg
      721 gtccggtgat ggatttcttc aatttcaggt aaagataatt ctcttgccag aacagttctc
      781 tttatcccca gttcttttaa aaacaggacc ccttccgtat ccgtgatcgc catctgtgta
      841 ctggcgtgga caggaagctc tggaaacata cgcctgatat gtgaaatcac accaatatcc
      901 tgtacaatga ccgcatccag tccccaataa taaaacgatt ccaaataaaa atctaattca
      961 tgcagttctt cattcttgaa cagtgtattg atcgttaagt aaactttctt cccaaataaa
     1021 tgtgcataat cgatcgctgc cttaagttct tcgtctgaaa aattttgtgc ataggctctt
     1081 gcaccgaatc tgtttccgcc aagataaacc gcatccgctc cggcgttaac cgcagcagta
     1141 aaacactcgt aatcaccggc cggagataaa agttctattt tacttttcat aaaaacccct
     1201 gtcattataa aaggctgtca tttctgacag cctctgtatt cgccaaaaat caaaagtgca
     1261 tccggcatta tctttttcgg tcacttattt cattttgata acttctgatc attttttcct
     1321 tgctttccag tttgatctgg gaagcaatca attcatgctt cagatcatat aattccctat
     1381 ccttcgcctc aatttcttct tccagcgctt caatctgttt tttcgcttta aaataatcat
     1441 cggctacatt cagctgcaat aaaacacttt gcatatcgag cggctgtctt ctgaaagaat
     1501 ccaccttact gtactctgca attttattat tgatgtatga tgctactttc tgtaagtatt
     1561 cttcgctttc gtaaccacac agggtaatta cttttccgcc gataataact tcggtatcat
     1621 tctttgaagc catgcgttat tt
//
LOCUS       DAOSYE010000569         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65136_length_1640_cov_2.434700, whole genome shotgun sequence.
ACCESSION   DAOSYE010000569 DAOSYE010000000
VERSION     DAOSYE010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65136_length_1640_cov_2.434700"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLU43_09325"
     CDS             <1..166
                     /locus_tag="PLU43_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HOO28654.1"
                     /translation="SKRFDIKPMYPEDACIQMELLGHSFFVFCNAETEQVNVVYKRKG
                     NTYGLIEPEL"
     gene            263..1399
                     /gene="mtnA"
                     /locus_tag="PLU43_09330"
     CDS             263..1399
                     /gene="mtnA"
                     /locus_tag="PLU43_09330"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOO28655.1"
                     /translation="MMDKEKNIMDYETVALDDEKKALVIIDQTKLPYKTVILELTEIK
                     DIWNAIYQLQVRGAPAIGVAAAIGIYITADRVQTESYEEFYEKFKEMKEYLNSSRPTA
                     VNLSWALNRMEQVCIQNKEKPVIQIKKALHDEAEEIKNEDIWSCRSIGEYGLSLVKPG
                     DGLLTHCNAGQLATSKYGTATAPMYLGQERGYHFRIFADETRPLLQGARLTAYELKEA
                     GLDVTLICDNMSASVMKEGLINAIFVGCDRVAANGDTANKIGTSVVAAVAKYYQVPFY
                     ICAPTSTIDRSLTDGSRIPIEQRPAEEVTEMWYRERMAPKDIKVFNPSFDVTDHELIT
                     AIITEYGIARPPYQESFQKIFERKYEQKTGKAMDIGVANAYSLC"
     gene            1488..>1640
                     /locus_tag="PLU43_09335"
     CDS             1488..>1640
                     /locus_tag="PLU43_09335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOO28656.1"
                     /translation="MSKKVVIAGACRTAIGTMGGTLSKTPAVELGAIVIKEALNRAGV
                     APEAVDQ"
BASE COUNT          517 a          280 c          444 g          399 t
ORIGIN      
        1 ttccaaaagg tttgatatta agccgatgta tccggaggat gcatgtattc agatggagct
       61 tttgggacat agtttttttg tattttgcaa tgcagagaca gagcaggtaa atgttgtata
      121 taaaagaaaa ggaaatacat acggtttgat tgagccggaa ttatagggat taagcagaaa
      181 tggaactcct atgccagtat cggcatggga gttcttaacg tagtgctgtt attggaaaaa
      241 gagtatagaa acaggggagc ggatgatgga caaagaaaaa aatattatgg attatgaaac
      301 agtggcactt gatgatgaaa agaaagcgct cgttatcatt gatcagacaa aacttccgta
      361 taagacggtt attctggaat tgacggaaat caaggatatc tggaatgcga tctatcagct
      421 tcaggtcaga ggagcaccgg cgatcggtgt ggcggcggca atcggtattt atattacggc
      481 agacagagta cagaccgaaa gctatgagga attttatgaa aaattcaaag aaatgaaaga
      541 gtatctgaat tcctccagac cgactgcggt gaatttgtcc tgggcgctta accggatgga
      601 gcaagtctgt attcaaaata aagaaaaacc ggttatacag atcaaaaaag cgcttcatga
      661 tgaggcggaa gaaattaaga acgaagatat ctggtcctgc aggtcgatcg gtgagtatgg
      721 gttatcactt gtaaagccgg gggacggatt attgacgcat tgcaatgcag gacagcttgc
      781 cacgtcaaaa tacggaacag ctacggcacc gatgtatctg ggacaggaaa gggggtatca
      841 tttcaggatc tttgcggatg aaacgcgacc gcttttgcag ggggcgcgtc tgactgccta
      901 cgaactgaag gaagcgggac tggatgtgac actgatctgt gataatatgt cggcatcggt
      961 catgaaggaa ggattgatca atgcaatatt tgtcggctgt gaccgtgttg cggcaaacgg
     1021 ggatacggca aataagatcg gaacatctgt tgtcgcggca gttgcaaaat attatcaggt
     1081 gcctttttat atttgtgcgc cgacttccac gatcgacaga agcttaacgg acggaagccg
     1141 gataccgatc gagcagcgtc cggcagaaga agttacggaa atgtggtatc gggagcgaat
     1201 ggcgccaaag gatatcaagg tctttaaccc cagttttgat gtgactgatc atgagctgat
     1261 cacggcgatt attacggaat acggaattgc ccgtccgcct tatcaggaat cctttcaaaa
     1321 aatatttgaa agaaaatatg aacagaaaac agggaaagca atggatattg gtgttgcaaa
     1381 tgcctattcg ctgtgctaaa atggataaag cgacaatgac aaaaaaataa caatcattgg
     1441 gaacccttgt tttacgggat tttaaacaat ttcatggagg taaggatatg tctaaaaaag
     1501 ttgttatagc aggtgcctgc cgtactgcga tcggtacaat gggcggaaca ttaagcaaaa
     1561 caccggcggt agagcttggt gcaattgtta tcaaagaggc attgaacaga gccggagttg
     1621 ctcctgaggc agttgaccag
//
LOCUS       DAOSYE010000570         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65188_length_1639_cov_2.908460, whole genome shotgun sequence.
ACCESSION   DAOSYE010000570 DAOSYE010000000
VERSION     DAOSYE010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65188_length_1639_cov_2.908460"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..411)
                     /locus_tag="PLU43_09340"
     CDS             complement(193..411)
                     /locus_tag="PLU43_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOO28657.1"
                     /translation="MKKKFKLEDLDCANCAAKMENEIKKIDGVSDANVSFMTQKLTVE
                     AEEEKFDEIMKKIIRICARVEPDCRILV"
     gene            complement(440..814)
                     /locus_tag="PLU43_09345"
     CDS             complement(440..814)
                     /locus_tag="PLU43_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO28658.1"
                     /translation="MEKNKSQDNQVECCDTFFVHEDSLRYVNDNMPAEEELYDLAELF
                     KVFGDSTRIRILFVLFEADVCVCDIAAALNMTQSAISHQLKILKQSKLIKNRREGKSV
                     FYFLADDHVRTIIAQGMEHIRE"
     gene            complement(955..1122)
                     /locus_tag="PLU43_09350"
     CDS             complement(955..1122)
                     /locus_tag="PLU43_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28659.1"
                     /translation="MKLIDYLKTQLKKDWKIMLVLTAASGKEPFYEQLGFICRPNEQL
                     GSGMSRWLLSE"
     gene            complement(1119..>1639)
                     /locus_tag="PLU43_09355"
     CDS             complement(1119..>1639)
                     /locus_tag="PLU43_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO28660.1"
                     /translation="DPDAAVYWMLRMLQGGEDPLYIARRLVRFASEDIGMADSRALQV
                     AVAAYQACHFLGIPECDVNLTHAVTYLSMAPKSNALYTACEACKDDLKSQRLEPVPLQ
                     LRNAPTKLMKDLSYGKDYEYAHNSEAKLTKMECFPESMKGKEYYHPTDSGEEKNVKER
                     LSYIKKFKEQIK"
BASE COUNT          415 a          430 c          258 g          536 t
ORIGIN      
        1 ccgcattccc cctgtttctt tcagatttcc gatattctcc gcaagcagca gccccacaaa
       61 cagcggtaat gtaatgagca cacgcaataa cacctgtttt tgtttttttg tcatgtagat
      121 aaactccttt tagcttttca aatttccaga tacatacccc ccggataatt cttctttatc
      181 cggggggtat gtttatacta agatcctgca atccggttcg accctggcgc agatccgaat
      241 gatctttttc atgatctcat caaacttctc ctcttccgct tccaccgtca gtttctgcgt
      301 cataaagctg acatttgcat cactgactcc gtctattttt tttatctcgt tttccatctt
      361 tgctgcgcag ttcgcgcagt ccagatcttc caatttgaat tttttcttca ttatgattcc
      421 ctctttcttt gtcattttct cattccctga tatgttccat tccctgtgca ataatcgttc
      481 tcacatggtc gtccgccaga aaataaaaaa ccgattttcc ttcccttctg tttttgatca
      541 gctttgactg ctttaagatt ttaagctgat gggaaatagc agactgcgtc atattcaaag
      601 cagcagcaat atcacagaca cagacatccg cttcaaataa tacaaacagt atccggatcc
      661 ttgtcgaatc cccgaatact ttgaacagct ccgccagatc atacagctct tcttccgccg
      721 gcatattatc atttacatac cgaagcgaat cttcatgtac aaaaaatgta tcgcagcatt
      781 ccacctgatt atcctgactc ttatttttct ccataaaacc acttctttca tttttatatt
      841 ctttcatatg aacatgtatt catatgttta tgataataca acctacagga actgtcaatc
      901 gttctttttg aaaaaattta ttataaaacc gcagctttat cccttttata cattttattc
      961 agataacagc cacctggaca tcccggaacc caattgctca ttcggtctgc atataaatcc
     1021 aagctgctca taaaacggtt cttttccgct ggctgccgtc aggacaagca tgattttcca
     1081 gtctttcttc agctgtgtct ttaaataatc aatgagcttc attttatttg ctccttaaat
     1141 ttttttatat aggataatct ttctttcaca tttttttctt ctccggaatc agtcggatga
     1201 taatactcct ttcccttcat cgactccgga aaacattcca ttttagtaag cttcgcctcc
     1261 gaattatgtg catattcata gtcctttcca taggacaggt ctttcatcag ctttgtcggt
     1321 gcgttgcgaa gctgaagcgg caccggctcc aaccgttgac tttttaagtc atccttgcat
     1381 gcctcacatg cagtataaag cgcatttgat tttggtgcca tggataaata ggtgaccgca
     1441 tgggtcaaat ttacatcaca ttccggaatc ccaagaaaat gacaggcctg ataagccgca
     1501 accgcaacct gcagcgcccg actgtctgcc attccaatat cctcgcttgc aaaccggaca
     1561 agccggcgcg ctatataaag cggatcctca ccgccctgca gcatacgcag catccagtaa
     1621 actgccgcat ccggatcac
//
LOCUS       DAOSYE010000571         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65273_length_1638_cov_2.085913, whole genome shotgun sequence.
ACCESSION   DAOSYE010000571 DAOSYE010000000
VERSION     DAOSYE010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65273_length_1638_cov_2.085913"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="PLU43_09360"
     CDS             <1..1620
                     /locus_tag="PLU43_09360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO28661.1"
                     /translation="WPVVSKTMRYLASEKQTVIFELDSPFMIVGERINPTGKKKLQEE
                     LKGGSFEIVNEFAVAQEAQGASLLDINMGMSGIDEKATMLKAIEEVTMKVSLPLVIDS
                     SHVDVIEQALRRYPGRALINSISFEKVKFEYLMPIAKKYGAMFILLPLSDEGLPKTLE
                     EKKTIIHQILKRADKLAISRNDIIVDGLVATVGANKSAAIETLETIRYCKEELKLPTI
                     VGLSNISFGLPERINVNEGFLTLAIQAGLTMAISNPSQQQLVACALATDMLLNKNNSD
                     IRYIRFINDLKEQYPDMAPMGTISSRGEQGLAAHKKVPDMTAADSKKESTESTGSVDK
                     IYKAVLSGNRKSIVAYTKEAVDSGKEARIVLDESLLPAINEVGVLFEKGKYFLPQLIA
                     SAEAMKLAIEYLEPMLMSVGKDRPQVTIVIATVKGDIHDIGKNLVSLMLKNYGFKVID
                     LGKDVAKEDIIKAAKENQAQIIALSALMTTTMPAMKRVVELAEAEGVKAQIIIGGAVI
                     TQEYADEIHADGYSKDAQEAVKLVQKLMKLL"
BASE COUNT          503 a          309 c          443 g          383 t
ORIGIN      
        1 tggcctgttg tttcaaaaac aatgcgttac ctggcatctg aaaagcaaac cgttatattc
       61 gaactggatt cgccttttat gatcgtagga gaacggatca atccgacagg gaaaaagaag
      121 ctgcaggaag agctgaaagg cggcagcttt gaaatagtca atgaatttgc cgtcgcgcag
      181 gaagcccagg gggcttcctt actggatatc aatatgggta tgagcgggat agacgaaaag
      241 gcgacgatgc tgaaggcaat cgaagaagta acaatgaaag taagccttcc gcttgtcata
      301 gattccagcc atgtagatgt cattgagcag gcactgcggc gttatcccgg tcgtgcactg
      361 atcaattcga tctcatttga aaaggtgaaa tttgagtatt tgatgccgat cgcaaaaaaa
      421 tatggtgcaa tgtttatttt actgccgctt tccgatgaag ggctgccgaa gacactggaa
      481 gaaaaaaaga cgatcattca tcaaattctg aaaagagcgg acaagcttgc gatcagcaga
      541 aatgatatta tcgtagacgg ccttgtggcg acggtcggtg ccaataaatc tgctgcgatc
      601 gagacgctgg agacgatccg gtactgtaaa gaggaactga agcttccgac gattgtcggt
      661 ttgtccaata tttcgttcgg gcttccggaa cgtatcaacg taaatgaggg cttcctgaca
      721 ctggctattc aggcgggtct cacaatggcg atttccaatc cttcacagca gcagcttgtg
      781 gcatgtgcat tggcaacgga tatgctctta aataaaaata attccgatat tcgatatatt
      841 cggtttatca atgatttgaa agagcagtat ccggatatgg ctccgatggg aacgatcagc
      901 agcagaggag aacagggact ggcggctcat aaaaaggtgc cggatatgac cgcggcggac
      961 agtaaaaaag agtcaactga atcaaccggg tcggtcgata aaatatataa agctgttctt
     1021 tccggaaaca ggaaatcgat cgttgcatat acaaaggaag cggttgacag cggaaaagaa
     1081 gcaagaatcg tgcttgatga aagccttttg ccggcaatta atgaagtggg tgttctgttt
     1141 gaaaaaggaa aatattttct cccgcagctg atcgcaagtg ccgaagcgat gaagcttgcg
     1201 attgaatatc tggagccgat gctgatgtcg gtcgggaaag acaggccgca ggtcacgatc
     1261 gttattgcaa cggtaaaggg tgatattcat gatattggta aaaatctcgt atcgctgatg
     1321 ctgaaaaatt acggatttaa agtgatcgat cttgggaaag atgttgcgaa agaggatatt
     1381 attaaagccg caaaggaaaa tcaggcgcag atcattgcac tttccgcact gatgacaacg
     1441 acgatgccgg cgatgaaacg agtcgtggag cttgccgaag cggaaggtgt caaggcacag
     1501 atcattatcg gcggtgcggt catcacacag gaatatgcgg atgagatcca tgcggacggt
     1561 tattcgaagg atgcacagga agcagtgaag cttgtacaga aactgatgaa attactataa
     1621 gaaaatgcaa aggagaat
//
LOCUS       DAOSYE010000572         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65472_length_1635_cov_2.656962, whole genome shotgun sequence.
ACCESSION   DAOSYE010000572 DAOSYE010000000
VERSION     DAOSYE010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65472_length_1635_cov_2.656962"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLU43_09365"
     CDS             complement(<1..496)
                     /locus_tag="PLU43_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOO28662.1"
                     /translation="MMEEMITDPKREVPKEGVAVTKKIRQEGPDIVRTIAAVFVVAVH
                     FYLNCGYYSEPLDNPILFIMTVARWLFLICVPLFMILTGYFKSEKTLSKSHYMGIVPI
                     MVSYVVISIIKAFACNYVYGTVYTFKYTVKNIANYQLAWYTGMYLSLMVLIPFLNRLW
                     KALVS"
     gene            679..>1635
                     /locus_tag="PLU43_09370"
     CDS             679..>1635
                     /locus_tag="PLU43_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOO28663.1"
                     /translation="MKKTLKFTMAAFLAFAAAIFFIPQTIVSADEVSLPVSEEINESD
                     SVGNVFYAGDSTVLDNTLAKKDVYMFGNSCKFNNSDISQDAIIAGNTISFKDTAIEGS
                     MRTAGYSILINNTTVSHNITAAANSIDIDSGSTCDAALLCGSSVSFDGTCGDLKVAAG
                     TVTINGVVTGDALISADKISIGPNAVINGNLKVESGKEPTISEKASIENLDFEKVENH
                     SEMIEKVSFGAMVAHKLLSRLYWIPALALIALTFCFIFGKALDGSGAMLKKKPAIMLG
                     SGAITLFALPVALILLCITYIGLPLAGLILLLMLPVMFFAVTF"
BASE COUNT          473 a          383 c          321 g          458 t
ORIGIN      
        1 tggacacaag cgccttccaa agcctgttca aaaacggaat cagcaccatc aatgacaaat
       61 acatccccgt ataccaggcc agttgataat tggcaatgtt ttttaccgtg tatttaaacg
      121 tatagactgt tccatataca taattacagg cgaacgcctt tataatagaa atcacaacat
      181 aggaaaccat aattggaaca attcccatat aatgagattt gctcagcgtc ttttccgatt
      241 tgaaatatcc ggtcagaatc ataaacaggg gcacacagat caaaaaaagc caccttgcaa
      301 cagtcatgat aaacaaaatc ggattgtcaa gcggttcgga ataatagccg cagttcagat
      361 aaaagtggac tgctacgaca aatacggccg ctatcgttct gacaatatcc ggcccctcct
      421 gcctgatttt tttcgttact gcgacgcctt cttttggcac ctctctttta ggatcagtta
      481 tcatctcttc catcatccgc atacctttta tcgattttct ttttctcttt attgtaccgg
      541 aatattctcc gttcgtaaag aaaaagaata atgattactt ttgtaatatg gatttatgac
      601 aatcgtgtct tttccatgca gcattgtttt gctatactca gcttataaac caaattctat
      661 gtggagggaa tcaaaaatat gaaaaaaaca ctaaaattca caatggcagc atttcttgcg
      721 tttgccgccg caatattttt tataccgcag acaatcgtca gtgcagacga agtctcgctg
      781 cctgtttccg aagaaataaa cgagtcggac agtgtcggaa atgtttttta cgcaggcgat
      841 tcgaccgttc ttgataatac gctggcaaaa aaagatgttt acatgttcgg aaacagctgc
      901 aaattcaata actccgatat ctcacaggat gccatcatcg ccggcaatac gatttctttt
      961 aaggatacgg ctattgaagg aagcatgcgc accgccggat attccatctt aatcaacaat
     1021 acaacagtct cccataatat taccgcagcg gcaaattcca tcgatatcga ctccggcagc
     1081 acctgtgatg cagcattact gtgcggcagt tccgtgtctt ttgacggtac atgcggtgat
     1141 ctaaaagtcg cagccggaac agtcacgatc aacggtgtcg ttaccggcga tgcactgatc
     1201 agcgccgata aaatatctat cggaccaaac gctgtcatca atggaaatct gaaagtggaa
     1261 agcggaaaag aaccgacgat cagcgaaaaa gcttccattg aaaatctgga ctttgaaaaa
     1321 gtagagaatc attcggaaat gatcgaaaaa gtatctttcg gcgctatggt cgcacataaa
     1381 ctgctttcca gattatactg gatccctgca cttgcattga tcgcactgac cttctgcttt
     1441 atatttggaa aagcattgga cggttccggc gcaatgctga agaaaaagcc ggctattatg
     1501 ctcggctccg gagccattac gctctttgcc cttccggtag cactgattct gctctgtatt
     1561 acttacatcg gcctgcctct tgccggactg atcctgctgc tcatgcttcc ggtcatgttc
     1621 tttgccgtta ccttt
//
LOCUS       DAOSYE010000573         1630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65806_length_1630_cov_2.662857, whole genome shotgun sequence.
ACCESSION   DAOSYE010000573 DAOSYE010000000
VERSION     DAOSYE010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65806_length_1630_cov_2.662857"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1107)
                     /gene="aroB"
                     /locus_tag="PLU43_09375"
     CDS             complement(1..1107)
                     /gene="aroB"
                     /locus_tag="PLU43_09375"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOO28664.1"
                     /translation="MSCKLEVTYQKKKSYDIIIENSYEKLPSVLTELFSQDQKLCIVT
                     DRTVEKLYADQIMKLTAPLFSHVEVYSFAAGEENKNLDTVKSVYRFLIEKKFGRKDVL
                     AALGGGVTGDLTGFVAATYLRGVDFFQLPTTLLSQVDSSIGGKTGVDFDQFKNMVGAF
                     KMPKLVFINTDTLKTLPARQFASGMAEVLKAGLIRDSIFYEWLITNFMEINEKDPAIM
                     EELISRSCGIKRTVVEKDPLEQNERALLNFGHTLGHAIEKYKDFSLLHGECVALGAVA
                     AAYISWKKELLSMEEYYEIRDMFVPFRLPISVDHILADEIVALTKSDKKAEGDHIKFI
                     LLKKIGKAFISKDVTEEEMLAALLEIEAKDAADD"
     gene            complement(1189..>1630)
                     /locus_tag="PLU43_09380"
     CDS             complement(1189..>1630)
                     /locus_tag="PLU43_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOO28665.1"
                     /translation="DEDEYEEPLPRKRKSIKSETLDDDYNEKPKAKATPKVTPMRSGK
                     NRTTAGGMEVCVIKPTTVEDAREITETLLANRTVVLNMEGLDVDIAQRIIDFTSGSTF
                     AISGNLQKISHYIFIITPRSVDISGDFQEILSGAFDVPALNNKF"
BASE COUNT          448 a          373 c          301 g          508 t
ORIGIN      
        1 ttaatcgtct gcggcatcct ttgcttcaat ttccaaaagg gctgcaagca tttcctcctc
       61 ggtcacatct ttggatataa aagcttttcc gatttttttc aataaaataa acttaatatg
      121 gtctccttcg gcttttttgt ctgattttgt gagagctacg atctcatcgg ccagtatatg
      181 atcaacagaa atcggaagcc ggaacggtac gaacatatcc cgtatttcat aatactcttc
      241 catgctgagc agctcttttt tccatgagat atatgcggct gccacagcgc cgagcgcaac
      301 acattcacca tgcagtaaag aaaaatcttt atacttttca atagcatgcc ccagtgtgtg
      361 gccgaaattc aaaagcgcac gttcattctg ctctaacgga tccttttcaa caacagtacg
      421 ttttattcca cagctgcgtg aaatcagttc ttccataata gccgggtctt tttcattgat
      481 ttccataaag tttgtaatca gccattcata gaatatactg tcacgaatca gaccggcttt
      541 caaaacttca gccattccgg aggcaaactg tcttgccgga agtgttttca gtgtgtccgt
      601 attgatgaaa acgagtttgg gcattttaaa cgcaccaacc atatttttga actgatcgaa
      661 atccacgccg gtttttccgc caatactgct gtcaacctgt gaaaggagtg ttgtaggaag
      721 ctgaaaaaaa tccacaccac gcagataagt ggctgccaca aaaccggtca gatcaccggt
      781 cacaccgcct ccgagcgctg caaggacatc ttttcgtccg aatttctttt cgatcagaaa
      841 acgataaacc gattttactg tatccagatt tttattttct tcgccggccg caaatgaata
      901 aacttccaca tgggaaaata gcggtgccgt cagcttcatg atctgatcag catataattt
      961 ttcaacagta cggtctgtaa caatacataa tttctgatcc tgactgaaca gctccgtaag
     1021 tacggagggc agtttttcat aggaattttc aatgataata tcatagctct tctttttttg
     1081 ataagtcact tctaatttgc aggacattcg ggtttctcct cttataaaat attttgaaaa
     1141 gtaaaaatgc tccctgcaat aaatgcagga agccatgtcg atacataatc agaatttatt
     1201 atttaatgcc ggaacatcaa aggcacctga caaaatttcc tgaaagtcgc cggatatatc
     1261 aacgcttctc ggtgtaatga taaaaatata atgtgaaatt ttttgcagat tgccgctgat
     1321 tgcaaatgtg gaaccggatg taaaatcaat gatcctttgt gcaatatcta catcaaggcc
     1381 ttccatattc agaacaaccg tcctgtttgc aagcaatgtt tccgtgatct ccctcgcatc
     1441 ttcaaccgta gttggcttga ttacacatac ctccatacca cccgctgttg tcctgttttt
     1501 gccggaacgc atcggtgtaa cctttggcgt ggcttttgcc ttaggttttt cgttataatc
     1561 atcatctaag gtttccgact tgatggattt tcttttacgc ggaagcggtt cttcatattc
     1621 atcctcatcg
//
LOCUS       DAOSYE010000574         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65984_length_1627_cov_3.087150, whole genome shotgun sequence.
ACCESSION   DAOSYE010000574 DAOSYE010000000
VERSION     DAOSYE010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65984_length_1627_cov_3.087150"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLU43_09385"
     CDS             <1..501
                     /locus_tag="PLU43_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28666.1"
                     /translation="INTLIAVICTIELLLFVFSINYIYITTKPPYIRRPWVYGMILTA
                     MGLLFIVRALINIIYYTWGRVDLSLPEIGVNIADLVISVFWIISGILLLVKKRKFHII
                     GILSYFHGSLLFVALIIFMAIQPLLCGTELIITDLFVIGFMSLLFIIPCILLLRKITD
                     PETSRP"
     gene            complement(473..910)
                     /locus_tag="PLU43_09390"
     CDS             complement(473..910)
                     /locus_tag="PLU43_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28667.1"
                     /translation="MTGNSKEKSPYEDIINLPHYVSDSRAHMSIHDRAAQFSPFAALT
                     GFDGEIKETARQTEQRIELNEDEKNILDEKLGIIQTHLNGRQEIQFAFFQPDEWKSGG
                     TYVSVKGIVKKIDRYGRVVVMQDGKRIPIEEIVDITGEMFQGL"
     gene            complement(949..>1627)
                     /locus_tag="PLU43_09395"
     CDS             complement(949..>1627)
                     /locus_tag="PLU43_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HOO28668.1"
                     /translation="HAWGFEPCTIADIKAYKPASNSIGSGQVLQSPYHYDKARLIVHE
                     MTDLLALDLVDKGFVTDQIVLTVGYDIENLNNAERREKYSGPVTTDRYGRKVPKHAHG
                     TANLNRRTSSAKLMINAVMGLYARIVDEDLLVRRIYVTANHVMREDAAEKEKPYEQLD
                     LFTDYEARQKEKEEEDAALLREKKMQLAILDIKKKYGKNAILKGMNLEEGAMTTKRNK
                     QIGGHKA"
BASE COUNT          385 a          388 c          284 g          570 t
ORIGIN      
        1 ataaataccc tcattgctgt aatctgtact attgagctat tattatttgt attttccata
       61 aattatatct atattaccac aaagccgccc tatatccgcc gcccatgggt ctatggcatg
      121 atcctcacag ctatgggtct gctgttcatt gtgcgtgcgt tgataaatat tatttattat
      181 acatggggca gggtggatct gtctttacct gaaatcggtg taaacatcgc agatctggtt
      241 atctctgttt tttggatcat cagcgggata ctgctgctgg taaaaaaaag aaaatttcat
      301 attataggaa tattatcata tttccacggt tcactgctgt ttgtggcatt gatcattttc
      361 atggccattc agcctctgct gtgcggcacg gaattgatta ttacggattt atttgtaatt
      421 ggatttatga gtctgctatt tattatcccc tgtattttac tgttgcgaaa aattacagac
      481 cctgaaacat ctcgcccgta atatctacaa tttcttctat cggtatcctt ttcccgtcct
      541 gcataaccac aacacgtcca tatctgtcaa ttttcttcac aatacctttc acagagacgt
      601 atgttccgcc gctcttccat tcatccggct gaaaaaatgc aaattggatc tcctgccggc
      661 cgttcagatg tgtttgtatg attcccagct tttcatctaa tatatttttt tcatcttcat
      721 tcagttctat cctctgctca gtctgtctgg ctgtttcttt gatctctccg tcaaaacctg
      781 tcagtgcagc aaacggagaa aactgtgcgg cacggtcatg tatggacata tgtgcacgac
      841 tgtcagaaac ataatgtggt aaattgatga tatcctcata cggacttttt tctttactat
      901 ttcctgtcat tctccgttcc tttcacagat aagacgctgc attatgcatt atgccttatg
      961 acccccgatc tgtttgtttc ttttggttgt catggcacct tcctcaagat tcatgccttt
     1021 tagtatcgca tttttcccgt attttttctt tatgtcaaga atcgcaagct gcatcttttt
     1081 ttcccgcagc aaagcagcat cttcttcttc tttttccttt tgcctggctt cataatccgt
     1141 aaataagtca agctgttcat acggcttctc tttttctgcg gcgtcttcac gcatgacatg
     1201 atttgccgta acgtatatcc ttctgactaa taagtcctca tccacgattc tggcatatag
     1261 acccatgacc gcattgatca tcagttttgc agaagaagtc cggcgattca gatttgcagt
     1321 tccatgtgca tgctttggaa ccttacgccc ataacgatct gtcgttacag gcccgctgta
     1381 tttttccctt ctctccgcat tatttagatt ttcaatatca tagcctactg tcaaaactat
     1441 ttgatctgtc acaaaaccct tatcaaccag gtcaagcgcc aaaaggtctg tcatttcgtg
     1501 cacgatcagt ctagccttgt cataatgata cgggctctgc agaacctgcc ctgaaccaat
     1561 actgttcgaa gctggcttat atgccttaat atccgctatt gtacagggtt caaaacccca
     1621 tgcatgg
//
LOCUS       DAOSYE010000575         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_65994_length_1627_cov_2.668575, whole genome shotgun sequence.
ACCESSION   DAOSYE010000575 DAOSYE010000000
VERSION     DAOSYE010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65994_length_1627_cov_2.668575"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLU43_09400"
     CDS             complement(<1..747)
                     /locus_tag="PLU43_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO28669.1"
                     /translation="MKKRLLSMIISAAMVATLLAGCGSSASEAENTEGTTEEVTEEAA
                     ETETAEAEETTDDSAASSGDVKITILNTKSEIQTQWEEMAEEYKELTGVEVEVSVTVG
                     DSPSEDITKRYASGEVPTIFMGDVQDIIMLDEYAADLSNEAWVADGGDQYGVKVNDRL
                     IGFPFCIEARGLMYNKTAIETVTGETFDPASVDTMDDLKTLLDKLVAGGMETPVALNM
                     EDWSLAGHWLTQVYEEQDGTAAGAQAFTDGL"
     gene            complement(1192..>1627)
                     /locus_tag="PLU43_09405"
     CDS             complement(1192..>1627)
                     /locus_tag="PLU43_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOO28670.1"
                     /translation="VCETRIGKIGMAICYDMRFPEIFRLMAKGGAQVICVPADFTMNT
                     GKDHWEALLRARAIENSCYIIAPGQTGQKPEFQAYGKSMVIDPWGNIIAKASDREQII
                     YAQIDLEYIDEIRRQIPSLHNIREDIYHLTSHNMKFYREEAL"
BASE COUNT          394 a          399 c          256 g          578 t
ORIGIN      
        1 taaaccgtct gtaaaagcct gtgctcctgc agcagttcca tcctgctctt catatacctg
       61 ggtaagccag tgtcctgcaa gggaccagtc ttccatgttc aaagctaccg gcgtttccat
      121 tccgcctgct accagcttgt caagcagtgt ctttaaatca tccattgtat ctacactggc
      181 aggatcaaat gtctcgcctg ttactgtttc aatggcggtc ttattataca tcaatcctct
      241 tgcttcaata cagaacggga atccgatcag tctgtcattc acctttacac catactgatc
      301 tccgccgtca gctacccatg cttcatttga aagatcagct gcatattcat ccagcatgat
      361 gatatcctgt acatcaccca taaagatggt cggtacttca ccggatgcat atctctttgt
      421 aatatcctca gacggtgaat cgccgactgt tacggatact tctacctcaa cacctgttaa
      481 ctccttgtat tcctctgcca tttcttccca ctgtgtctga atttcactct ttgtgttaag
      541 aattgttatt ttcacatctc ctgaagaagc agcggagtcg tccgtcgttt cttctgcttc
      601 agctgtttct gtttcagccg cttcttccgt cacttcctct gttgtacctt ccgtgttttc
      661 cgcttctgac gcactgctgc cgcagccggc taacagtgtc gctaccattg cagcggaaat
      721 aatcatactt aataacctct ttttcatttt tttcctcctt ctaattaatg atgcctcata
      781 atttccttta catttttgaa acgtttcaat ttgtaattat aaaatatatc ttttttatgc
      841 tttagtcaat atattttcgc atatttttgt gcacttttaa tatttttgtt aattttaaat
      901 aaatatagtc ttctcgaatt atctataatt atgaattaat tgcgttcttt ttatatgttg
      961 tgctcaattt ttacattatt tttgaatcgt tttatttatt gtcataattt taacgaaaac
     1021 ataagggcgg tttcgccaac tacttattgg aaaaacagcc cttatttata tattttctgt
     1081 tattaatcat taagtcttct ttacagaagc tgctccacga catttggaat tctcaaccgt
     1141 actacctggt caacaagcag cttcaatttt ccagcaagtc gttttaatat atcataaagc
     1201 ttcctctctg taaaatttca tattgtgact tgtcagatga taaatatcct ctcggatatt
     1261 atgaagggac ggaatctgcc tgcgaatttc atcaatatat tccagatcaa tctgagcata
     1321 aatgatttgt tctctgtcac ttgctttcgc aatgatattt ccccacggat caatgaccat
     1381 ggactttccg tacgcctgaa actccggctt ctgcccggtc tgcccaggcg ctattatata
     1441 acagctgttt tctatcgccc ttgcgcgaag cagcgcttcc caatgatctt ttccagtatt
     1501 catcgtgaaa tctgccggaa cacaaatgac ctgcgccccg cctttcgcca tcagtctgaa
     1561 tatttccggg aatcgcatat catagcaaat cgccattcct attttgccga tcctggtttc
     1621 acatact
//
LOCUS       DAOSYE010000576         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66246_length_1623_cov_3.059311, whole genome shotgun sequence.
ACCESSION   DAOSYE010000576 DAOSYE010000000
VERSION     DAOSYE010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66246_length_1623_cov_3.059311"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLU43_09410"
     CDS             <1..336
                     /locus_tag="PLU43_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO28671.1"
                     /translation="DELGDPLINSSFALALELATQEEIDIIKKYAFKVNDVMKAYFLQ
                     ADIELVDFKIEFGRYKGQIILADETSPDTCRLWDVNTHEKLDKDRFRRDLGNVEEAYQ
                     EVFRRLGLA"
     gene            343..>1623
                     /gene="purB"
                     /locus_tag="PLU43_09415"
     CDS             343..>1623
                     /gene="purB"
                     /locus_tag="PLU43_09415"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOO28672.1"
                     /translation="MMTDRYVSPLSERYASKEMQYIFSPDMKFRTWRKLWIALAETEK
                     ELGLGITEEQIDELKAHAEDINYDVAKEREKLVRHDVMSHVYAYGEQCPKAKGIIHLG
                     ATSCYVGDNTDIIIMTEALKLVRKKLINVIAELADFADKYKALPTLAFTHFQPAQPTT
                     VGKRATLWIQEFLLDLEELDFVLGSMKLLGSKGTTGTQASFLELFDGNQELVDRIDPM
                     IAAKMGFQECYPVSGQTYSRKVDTRAANVLAGIGASAHKFSNDIRLLQHLKEVEEPFE
                     KNQIGSSAMAYKRNPMRSERIASLSRYVMVDALNPAITSASQWFERTLDDSANKRLSI
                     PEGFLATDGVLDLCLNVVDGLVVYEKVIEKRLRSELPFMATENIMMDAVKAGGDRQEL
                     HERIRELSMEAGENVKKKGLENNLLELIAADPVFH"
BASE COUNT          468 a          299 c          458 g          398 t
ORIGIN      
        1 gacgagcttg gcgatccgct gatcaacagt tcctttgcgc tggcacttga gcttgccaca
       61 caggaagaga tcgatatcat caagaaatat gcatttaaag tcaatgatgt aatgaaggca
      121 tattttctgc aggcagatat tgagcttgtg gactttaaga tcgagttcgg cagatataag
      181 gggcagatca ttcttgcaga tgagacatcg ccggatacat gccgtctttg ggatgtaaac
      241 acgcatgaaa agcttgataa ggaccgcttc agaagagacc ttggcaatgt ggaggaagct
      301 tatcaggaag tatttagaag actgggttta gcataggaga aaattatgac agacaggtat
      361 gtaagtccgc tgtcggaaag atatgcaagt aaggaaatgc agtatatttt ttcaccggat
      421 atgaaattca gaacatggag aaagctttgg atcgcattag ccgagacaga aaaggaactg
      481 ggacttggca ttacagaaga acagatcgac gaactgaagg cacatgcgga agatatcaat
      541 tatgatgtgg caaaggaacg tgagaaactt gtgcgtcatg atgtgatgag ccatgtgtat
      601 gcttatggag aacagtgtcc gaaggcaaag ggaatcattc atctgggggc aacctcctgc
      661 tatgtgggtg ataatacgga tattattata atgacggagg ccctgaagct tgtcagaaaa
      721 aaattgatca atgtgatcgc agaactggcg gatttcgctg ataaatataa agcactgccg
      781 acacttgcat ttacgcattt tcagccggcc cagccgacaa cggtcgggaa gcgggcaacg
      841 ctctggatac aggaatttct gcttgatctg gaggaactgg attttgtact cggctctatg
      901 aagctgcttg gttccaaagg tactaccggg acgcaggcga gctttttgga actgtttgac
      961 ggcaatcagg agctcgttga caggatcgat ccgatgatcg ccgctaaaat gggctttcag
     1021 gaatgttatc cggtgtcagg acagacgtat tcgcgtaaag tggatacaag agcagcaaat
     1081 gtgctggcag ggattggggc aagtgcgcat aagttttcga atgatatccg tctgctccag
     1141 catttaaagg aagtggaaga gccgtttgaa aaaaatcaga tcggatcgtc cgcaatggct
     1201 tataaaagaa atccgatgcg ttctgagcgg atcgcctcat tgtccagata tgtcatggtc
     1261 gatgcactca atccggcaat tacttcggca tcacagtggt ttgagcggac gcttgatgat
     1321 tcggcaaata agcggctgag tattccggaa ggatttctgg cgactgacgg agtgctggat
     1381 ctttgtctga atgtagttga cggacttgtt gtatatgaaa aggtcattga aaagcggctg
     1441 cgcagtgagc tgccgtttat ggcaactgaa aatattatga tggatgcggt gaaggccggc
     1501 ggagacagac aggagctgca tgagcggatc agggagcttt ccatggaagc cggtgagaac
     1561 gtaaagaaaa aagggcttga gaataacctg cttgaactga tcgcggccga tcctgttttt
     1621 cat
//
LOCUS       DAOSYE010000577         1622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66292_length_1622_cov_4.597958, whole genome shotgun sequence.
ACCESSION   DAOSYE010000577 DAOSYE010000000
VERSION     DAOSYE010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66292_length_1622_cov_4.597958"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..707
                     /locus_tag="PLU43_09420"
     CDS             234..707
                     /locus_tag="PLU43_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28673.1"
                     /translation="MAFYEPVAAEEISYTAIDGTWFMSTFPLSGVLFGNRTELWWDHP
                     VIQSLEMVTDLLDISGLLKTFARELSLGKRMRADLAMMLLHSPKLILPDEPTLGLDVV
                     AKRQMIDFLKKINRENGVHEYEADLSKIPIKTLMSFCLNLMGSVMLRSRKHRLNR"
     gene            720..1505
                     /locus_tag="PLU43_09425"
     CDS             720..1505
                     /locus_tag="PLU43_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28674.1"
                     /translation="MKVGNRGADYDPDASFPSALLPYRKKMQMVFQDPYSSLNPRMKI
                     KAVLKEELLFHHICTAEEIDEKIHEIIGDVGLPESALERYPSEFSGGQRQRIGIARAL
                     AVNPEFIIADEPVSALDVSIQAQILNLLLHLQKEFGLSILFISHDLRVVNYIADRVAV
                     MYLGQIVELGTAQQIYENSLHPYTEVLLKSAPGLDPRIQTEKPVIEGNPPSPVDLLPG
                     CRFCKRCRYAEGICFEEEPKLLETEPGHFCACYRYVSKGELYG"
     gene            1498..>1622
                     /locus_tag="PLU43_09430"
     CDS             1498..>1622
                     /locus_tag="PLU43_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOO28675.1"
                     /translation="MDNYIITVARGFGSGGKQIALKLAGELGIECYENRILYLAS"
BASE COUNT          457 a          319 c          412 g          434 t
ORIGIN      
        1 agtagcaaaa aaaataaaaa gtactgataa atcggtactt gacttaaaga ggaggaaatc
       61 cctatgatga aatatttgaa aaccgtgcag ttatctgcac ttgaaaaaac aaatggagga
      121 attgtttatc ggccgcgggc aacttgagca tctgtttatg cagccgctgt ctttgaaggt
      181 tcagctgtct acatgcgggt ttaccccgtt cacgggcata tcttgtgtcc agcatggctt
      241 tttacgaacc tgttgccgct gaagaaattt cctatacggc tatagatggg acctggttta
      301 tgagcacgtt cccattgtca ggggtactgt tcggcaaccg tacagaactt tggtgggatc
      361 atccggtcat tcaaagcctt gaaatggtga cagacctttt ggatattagc ggtcttctca
      421 aaacatttgc aagagaattg agccttggca agcggatgcg cgctgatctt gcaatgatgt
      481 tgctgcattc gccgaagctg attttgcctg atgaaccgac gcttggactt gatgttgtag
      541 ccaagcgtca gatgatcgac tttcttaaga agatcaatcg tgagaacggc gttcacgaat
      601 atgaagcaga cctcagcaaa atcccaataa aaacgttgat gagtttttgt ctgaatttga
      661 tgggatcagt gatgttgaga tcaagaaagc accgattgaa caggtaattg ccggtgttta
      721 tgaaagttgg aaatagagga gcagattatg atccagacgc cagttttccg tcagcgcttc
      781 ttccttatcg gaaaaaaatg cagatggtat tccaggatcc ttattcttcc ctgaatccga
      841 gaatgaagat caaagccgtt ctgaaggaag aattactgtt ccatcatatt tgtacagcag
      901 aagagatcga tgaaaaaata catgaaatta tcggagacgt aggcctgccg gaatccgcac
      961 tggaaaggta tccaagtgaa ttttccggcg ggcagcgcca aaggatcggt attgcgaggg
     1021 cgctggccgt aaatccggaa tttataatcg cagacgaacc tgtttccgca ctggatgtat
     1081 ccatacaggc acagattctg aatctgcttt tgcatctgca aaaagagttt ggattatcga
     1141 ttttatttat ttcacatgat ctgcgggtag taaattatat tgccgacaga gtggccgtga
     1201 tgtatcttgg acagatcgtt gaacttggaa ccgcacagca gatttacgaa aacagcctgc
     1261 atccttatac ggaggtgctg ctgaaatctg caccgggact tgatccgaga atacagacag
     1321 agaagcctgt gatcgaggga aatccgccga gtccggtcga tctgctgccg ggctgcagat
     1381 tctgcaaaag atgcagatat gccgaaggga tctgttttga agaagaacca aagcttttgg
     1441 aaacagaacc gggtcatttt tgtgcctgct atcgttatgt gagtaaagga gagctttatg
     1501 gataattata taatcaccgt tgcccgtggg tttggaagcg gcggaaaaca gatcgcgctg
     1561 aagcttgccg gggagcttgg catagaatgc tatgaaaatc gtattttgta tcttgcgtcg
     1621 ca
//
LOCUS       DAOSYE010000578         1622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66333_length_1622_cov_2.292278, whole genome shotgun sequence.
ACCESSION   DAOSYE010000578 DAOSYE010000000
VERSION     DAOSYE010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66333_length_1622_cov_2.292278"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1622
                     /locus_tag="PLU43_09435"
     CDS             <1..>1622
                     /locus_tag="PLU43_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOO28676.1"
                     /translation="EAVLLEPGNLTACGRMLTAVEKRLEKSASVVMYLENPQLWSAED
                     PALYTLLIETLNEDGTISEIVFLPVGFRQFEIKDRLMRLNGKRIVLKGVNRHEFNSLR
                     GRVPSREDMIKDIVTMKQNNINAIRTSHYPNDSMLYALCDEYGLYMIAENNMESHGVW
                     DLFVRGFLNAGEVVPGDKEEWLANMLDRVNSTYQRDKNHPAVLIWSCGNESYGGKVIY
                     EMSNRFRQLDKTRLVHYEGICHDRRYPETSDMESQMYPPAAAIKEYLAKNRNKPFICC
                     EYLHAMGNSCGAMEKYTDLTDTEPLYQGGFIWDYIDQSLTKNDRYGNEFQAYGGDFDD
                     RPNDGNFSGNGIVYGRNREPSPKMQTIKYNYQNISVMFENDMVKIVNKYLFTNTNIFQ
                     CVVVLERDGHFVCDCELNTDVKPLSEKSYKLPLMLPKEAGEYVWTVSFRLRHDTLYAG
                     CGYEVAFGQYVQSVPKIREASETEKTGGFQVIRGANNIGIKGESFELLFSELLGGLVS
                     YRYGGKELLKSVVKPNFWRAPTDNDYGNLMPCR"
BASE COUNT          481 a          303 c          443 g          395 t
ORIGIN      
        1 gaagcggtgc tgctggagcc cggcaacctc acggcctgcg gcaggatgct gacagctgtt
       61 gaaaaaaggc tggagaaaag tgcgtctgtt gtaatgtatt tagagaatcc gcagctgtgg
      121 agtgcggaag atccggcatt atatacactg ctgatcgaaa cgctcaatga agatgggaca
      181 atatcggaaa tcgtattttt accggttggt ttcagacagt ttgaaataaa ggaccggctg
      241 atgcggctca acggaaaacg gatcgtattg aaaggagtga accggcatga gttcaattcc
      301 ctgcgcggac gtgtaccgtc aagagaggat atgatcaaag acatcgttac gatgaagcag
      361 aacaatatca atgcgatccg cacaagccat tatccgaatg attccatgct gtatgcgctg
      421 tgtgatgagt acggtctgta tatgatcgca gaaaacaata tggagtcaca tggtgtctgg
      481 gacctgttcg taagaggttt tctgaatgcc ggcgaagtag ttccgggaga taaggaggag
      541 tggctggcaa atatgctgga tcgtgtcaac tcgacatatc agcgtgacaa aaaccatcct
      601 gccgtactga tctggtcatg cggcaatgaa tcctacggag gaaaagtcat ttatgaaatg
      661 tcgaaccggt ttcggcagct ggataaaacg agacttgtac attatgaagg gatctgccat
      721 gacaggcggt atccggaaac aagcgacatg gaaagccaga tgtatccgcc ggctgctgcc
      781 attaaggaat atcttgcaaa gaacaggaac aagccgttta tctgctgtga gtatctgcat
      841 gcaatgggca attcctgcgg agccatggaa aaatatacgg atctgacaga tacggaaccg
      901 ctttatcagg gcggctttat ctgggattat attgatcagt cgcttacaaa aaacgatcgt
      961 tacggaaatg aatttcaagc atatggcggg gatttcgacg accgtccgaa tgatgggaac
     1021 ttcagcggaa acggaatcgt atacggcagg aacagggagc cgtcacccaa aatgcagaca
     1081 ataaaatata attaccagaa tatcagtgtg atgtttgaaa atgatatggt caaaattgtg
     1141 aataaatatt tgtttacaaa tacaaatatt tttcaatgtg tggttgtact tgaaagggac
     1201 ggacattttg tgtgtgattg tgaattgaat acagatgtga agcctctttc tgaaaaaagt
     1261 tataaattac cgttaatgct gccgaaggaa gccggagaat acgtgtggac ggtatctttc
     1321 cgcttaaggc atgacacgct ttatgcaggc tgcggatatg aagtggcatt cggtcaatat
     1381 gtgcagagcg ttccaaaaat cagggaggca tcggaaacag aaaagacagg aggcttccag
     1441 gtcattcgcg gggcaaataa tatcggtata aagggagaga gttttgagct gctgttttcc
     1501 gagctgctcg gcggtcttgt atcataccgg tacggcggga aagaattgct taaaagtgtg
     1561 gtaaagccta atttctggcg tgcgccgaca gacaatgatt atgggaatct gatgccctgc
     1621 cg
//
LOCUS       DAOSYE010000579         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66358_length_1621_cov_3.745849, whole genome shotgun sequence.
ACCESSION   DAOSYE010000579 DAOSYE010000000
VERSION     DAOSYE010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66358_length_1621_cov_3.745849"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLU43_09440"
     CDS             <1..1025
                     /locus_tag="PLU43_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28677.1"
                     /translation="VDTLRNSSCGTGAFQVSEFNPGVSVKYTKNENYWQEGKPYLDAV
                     EMYIVDEPTTRASAFEAGEYDIILMNNLTVANDLISSGNYVLERNKSGQGLVMTGLIP
                     NSVNEGPFQDARVRRAMCYAIDSQALSDAFGYGLLTTLNQWAAPGAVTYDDSLAGMDY
                     DPEKAKELLTEAGYPDGFTTTLTTDAGNKDYFTAAANMLDEVGIHCEINLVDESAQVN
                     LYSTGTWDGIMGHFASIAPDLGLYMGRHLDPDGAFYADGIQHPEDHLTLLEEIRTAPT
                     TEEKIEKEYEMQELIYNGETGSALFGKPLFVQFEPVIKYDYVKDDNRSVDHVATWSIE
                     NCWLDK"
     gene            1101..>1621
                     /locus_tag="PLU43_09445"
     CDS             1101..>1621
                     /locus_tag="PLU43_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28678.1"
                     /translation="MDNKLFKMIIKRIGQSIIVIFIVTLLVFLLMQLVPGDPIVNFLG
                     ANATDDQIAYYTKLFGYDQPVLIQYGKWIIGLFHGEMGRSVALQKDITEIIFQRLGVT
                     LSIVFPAFFIAVILGVVLGIVAAKHRGTPADTVISFFANIGMSMPMFWFGMILILVFA
                     LKLGILPTSGSVAL"
BASE COUNT          466 a          290 c          406 g          459 t
ORIGIN      
        1 atgttgacac attgagaaat tcatcatgcg gaacaggagc tttccaggta tccgagttta
       61 atcctggagt ttctgtaaag tacacaaaga atgaaaatta ctggcaggaa ggaaagccgt
      121 atctggatgc tgttgaaatg tatatcgttg atgaaccaac aacaagggcc tctgcctttg
      181 aagcgggaga atatgatatt attctgatga ataaccttac ggttgcaaac gatctgatca
      241 gttccggaaa ttatgtgctt gaaagaaata aatcaggaca gggacttgtt atgactggtc
      301 tgattccaaa cagtgtgaat gaaggaccgt tccaggatgc aagagtcaga agagctatgt
      361 gctatgcgat cgattcacag gcactcagtg atgcgttcgg atacggactg ctgacgacgt
      421 taaatcagtg ggcggctcca ggtgcagtaa catatgacga ttctctggca ggcatggatt
      481 atgatccgga aaaggcaaag gagcttctga cagaagccgg atatccggat ggctttacaa
      541 ctacgctgac aacagatgca ggaaacaagg attattttac ggctgcagca aatatgctgg
      601 atgaagtagg tatccactgt gagataaatc ttgttgatga aagtgcacag gtcaatttgt
      661 actcaacggg tacatgggat ggtattatgg gacattttgc atcgatcgca cctgatcttg
      721 ggctttacat gggccgccat cttgatccgg acggagcttt ctacgcagac ggtattcagc
      781 atccggaaga tcatttgaca ttgctggaag aaatccgtac ggcacctaca acagaagaaa
      841 agatagaaaa agaatatgaa atgcaggaac tgatctacaa tggcgaaaca ggcagcgcat
      901 tatttggaaa accgttgttt gttcagtttg agccggtcat caagtatgat tacgtgaaag
      961 acgacaacag aagtgtggat catgtggcaa catggagcat tgagaattgc tggcttgaca
     1021 agtaaatatt ttaagtatgg aacgttaagg gaggcggtca tcaggaccgc ctccatactt
     1081 aaaaaactgg aggttattaa gtggacaaca aacttttcaa gatgataata aaacggatcg
     1141 ggcaatcgat tattgttatc tttattgtta ctttattagt atttcttcta atgcagcttg
     1201 tgcctggaga tccaatcgtt aattttttgg gtgccaatgc gaccgatgac caaattgcat
     1261 attatacaaa attatttggt tatgatcagc cggtattgat ccagtacggg aaatggatca
     1321 ttgggctatt ccatggagag atgggacgtt ccgtggcatt gcagaaggat attactgaaa
     1381 tcatattcca aagactgggt gtcaccttat cgatcgtttt cccggcattt ttcattgcgg
     1441 tcattcttgg agtggttctt ggtatcgttg cggcaaaaca cagaggaacg ccggccgata
     1501 ctgtgatttc tttttttgcg aatatcggta tgtccatgcc tatgttctgg tttggtatga
     1561 ttttgatttt ggtatttgcg ctaaagcttg gtatattgcc tacatctggt tcggttgccc
     1621 t
//
LOCUS       DAOSYE010000580         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66688_length_1616_cov_3.526586, whole genome shotgun sequence.
ACCESSION   DAOSYE010000580 DAOSYE010000000
VERSION     DAOSYE010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66688_length_1616_cov_3.526586"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1250
                     /locus_tag="PLU43_09450"
     CDS             168..1250
                     /locus_tag="PLU43_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28679.1"
                     /translation="MLEVSIRKKLDNFTLETAFSLETGCMGILGASGCGKSVTLKCIA
                     GILTPDEGTIRLNGKVLFDSAKKINIRPQERRIGYLFQNYALFPNMTVSENIAAGFRA
                     EHMKRGDRRELCAARVKEMLVRFSLTELADRYPKHLSGGEQQRTALARLLVGEPEVLL
                     LDEPFSALDAYLKEELQLELLETMQRLLMPTVLVTHSRDEVYRLSSRLMIMDRGMAER
                     PDDTKKLFSSPGSMMAAKLTGCKNIAHVTKCSDEEYFAGDWNIRFPVSASKADYIGIR
                     AHDFVPEKEVEDGGLYIPVKISVIGISEGPFEWNAMIKSSGNPGQKMLWWKVSKEVIK
                     TKAEIKETKQLYIRKTGILYLMEKAL"
     gene            1383..1517
                     /locus_tag="PLU43_09455"
     CDS             1383..1517
                     /locus_tag="PLU43_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28680.1"
                     /translation="MSETPKKIKEMMTEIDKLKENFRRIKSGSEQWINRQEVRNKKAI
                     "
BASE COUNT          508 a          283 c          444 g          381 t
ORIGIN      
        1 gcggtggcgg ccggtgatat ggaactggcc tatcaatatg tactcgtgct tgtctgtatt
       61 tcgtttctga tggttcttgg catgaattat tttggaatga agggaaatgc aagaatgcgc
      121 cgctgacagg aacatcgaac tgtattttaa tgaccaggag tgacaaaatg cttgaagtat
      181 cgatcagaaa aaaactggat aatttcacac tggagacagc gttttcgctc gaaacaggct
      241 gcatgggcat tttaggggct tccggctgtg gcaagagcgt aacattaaaa tgtatcgcag
      301 ggatcctgac acctgatgaa ggaacgatca ggctgaacgg caaagtgctt tttgattccg
      361 caaagaagat caatatcaga ccgcaggaga ggcggatcgg atatttgttt caaaattatg
      421 cactttttcc gaatatgacg gtatcggaaa atattgcggc cggatttcgg gcggaacata
      481 tgaaaagagg agaccgcagg gaattatgcg cggccagggt aaaagaaatg cttgtgcgtt
      541 tttcgcttac ggagcttgcg gaccgttatc cgaagcatct gtcaggagga gaacagcagc
      601 gtacggcact cgcaaggctg cttgtaggag aaccggaggt gctgctgctt gacgaaccgt
      661 tttccgcatt ggacgcatat ctgaaagagg aattgcagct ggagcttttg gaaacaatgc
      721 agcggctttt gatgccgacc gtgcttgtaa cgcacagccg ggacgaagtt tatcgcttaa
      781 gcagccggct catgatcatg gacagaggta tggcggaacg tccggacgat acgaagaagc
      841 tgttttccag tcccggaagc atgatggcgg caaagctgac aggctgtaaa aatatcgcac
      901 atgtgacaaa atgttcggat gaggaatatt ttgccggaga ctggaatatc cggtttccgg
      961 tatccgcatc taaggcggat tatatcggga ttcgtgcaca tgattttgtg ccggagaaag
     1021 aagtggaaga tggaggcctg tatataccgg taaagatatc ggtcattgga atttcagaag
     1081 ggccatttga atggaatgcc atgataaaaa gcagtgggaa tccgggtcag aaaatgctct
     1141 ggtggaaagt cagcaaggaa gtcataaaga caaaggcgga gatcaaagag acgaagcagc
     1201 tttatatcag gaaaacaggg attctgtatt taatggaaaa ggcgttatag aaagcctgca
     1261 agtttgttca gtgaactaac aaaatgtatc atataaaatc atttttttaa taattttact
     1321 ggaaaaaaat atgtgattgt gttagtataa aaatatgggg agcagcttgc ggaggaaaaa
     1381 agatgagtga aacgcctaaa aaaattaaag agatgatgac ggaaatcgac aagctgaagg
     1441 aaaatttcag aagaatcaaa agtggttccg aacaatggat caacagacag gaagtcagaa
     1501 ataaaaaagc catatgatat cagcagcaaa aagccgacgc ataaaatgca tcggcttttt
     1561 acagtatgac gggcatgtcc cgaaccttgg gtgaaagtcc caaagggcgt agttgc
//
LOCUS       DAOSYE010000581         1614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_66859_length_1614_cov_2.890956, whole genome shotgun sequence.
ACCESSION   DAOSYE010000581 DAOSYE010000000
VERSION     DAOSYE010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66859_length_1614_cov_2.890956"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1614)
                     /locus_tag="PLU43_09460"
     CDS             complement(<1..>1614)
                     /locus_tag="PLU43_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOO28681.1"
                     /translation="VTSTTVFIGEDGNLLEEKAKNCNVLRVNNPILTNTDLLKIKTMK
                     AEGFHVEVVPITYYKNTSLEKAIDRMFIEVDRVYKDGANILILSDRGVDENHVAIPSL
                     LAVSALQQHLVETKKRTSVAMILESGEPRDVHHFATLLGYGACAINPYLALETVKELI
                     DNDMLDKDYYAAVDDYNYAVLHGIVKIASKMGISTIQSYEGSKIFEAIGIDQEVIQKY
                     FTNTVCRVGGLSLKDIEKQVDELHSMAFDPLGLENDLTLDSPGNHKMRSGAEEHMYNP
                     ATIHLLQESVKRGDYDMFKQYTALVDDENGIKNLRGLMGFNYAKKPIPIDDVESVDSI
                     VTRFKTGAMSYGSISQEAHETLAIAMNMIHGKSNTGEGGESLDRLTIGPDGFNRCSAI
                     KQVASGRFGVTSRYLVSAQEIQIKMAQGAKPGEGGHLPGKKVYPWVAKTRHSTPGVGL
                     ISPPPHHDIYSIEDLAQLIYDLKNANKDARISVKLVSEAGVGTVAAGVAKAGAQVILI
                     SGYDGGTGAAPKTSIHNAGLPWELGLAETH"
BASE COUNT          374 a          456 c          339 g          445 t
ORIGIN      
        1 atgcgtttcc gcaagaccaa gctcccatgg gagtccggca ttatggatag aagtctttgg
       61 tgctgctccc gtaccgccgt catagcctga gatcagaatg acctgcgcac cggcttttgc
      121 aacaccggcc gcaaccgttc cgactcctgc ctctgatacg agtttcacgg aaatccttgc
      181 gtctttattt gcatttttca gatcatagat cagctgtgcc agatcctcga tcgaataaat
      241 atcatgatgc ggcggcggag agatcaatcc cacccctggt gtcgaatgtc ttgttttggc
      301 tacccacgga taaaccttct tccccggcag atggcctcct tcaccgggct ttgctccctg
      361 tgccattttg atctggatct cctgcgcgct tacaagatat ctgcttgtta caccaaaacg
      421 tcccgacgct acctgcttga tagcggagca tctgttaaac ccgtccggtc cgatcgtcag
      481 ccggtcaaga ctttcaccgc cttctccggt attggacttt ccatggatca tattcatcgc
      541 gatcgcaagc gtttcatgcg cttcctgtga aatggagcca taagacatgg caccggtctt
      601 aaaacgcgtc acgatcgagt caacagattc gacgtcgtcg atcgggatcg gcttcttcgc
      661 ataattgaag cccatcagac cccttaaatt tttaatgccg ttctcatcat ccacaagcgc
      721 cgtatactgc ttgaacatat catagtcacc gcgcttcacc gactcctgca gtaaatgaat
      781 cgttgccgga ttatacatat gctcctcggc cccggatctc atcttatggt tccctggact
      841 gtccagtgtc agatcatttt caagcccaag aggatcaaat gccatggaat gcagttcatc
      901 gacctgtttt tcaatatcct ttaaggacag gccgccgaca cggcagaccg tatttgtaaa
      961 atatttttgg atcacttcct ggtcaatgcc gatcgcctcg aagatcttcg aaccctcata
     1021 agactggatc gtggaaatac ccattttgga agcaattttc acaataccat gcagcaccgc
     1081 ataattataa tcgtctaccg ccgcataata atccttgtca agcatatcgt tatcgatcag
     1141 ttccttgact gtttccaaag ccaggtacgg attgatcgca caggcaccgt agccgagcag
     1201 ggtcgcaaaa tgatgtacgt ctcttggctc tcctgactca aggatcattg ccacgctcgt
     1261 ccgcttcttt gtttctacca gatgctgctg cagcgcggat accgctaaca atgacgggat
     1321 cgccacatga ttttcatcaa caccccggtc agacaggatc aggatattgg ctccgtcctt
     1381 ataaactctg tccacttcaa tgaacatacg gtcgatcgcc ttttccaggc ttgtattttt
     1441 atagtaagtg atcggcacga cttccacatg gaagccttcg gccttcatcg tcttgatctt
     1501 taaaaggtcc gtattcgtca gaatcggatt attcacacgc agtacattgc agttcttcgc
     1561 tttttcctcc agcagattcc cgtcttcccc gataaagaca gtcgttgaag tcac
//
LOCUS       DAOSYE010000582         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67104_length_1611_cov_2.533419, whole genome shotgun sequence.
ACCESSION   DAOSYE010000582 DAOSYE010000000
VERSION     DAOSYE010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67104_length_1611_cov_2.533419"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLU43_09465"
     CDS             complement(<1..356)
                     /locus_tag="PLU43_09465"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO28682.1"
                     /translation="MYPWITFTLSGSKYTIASYTLFAFIGALTGIFLSLPLLKREGIS
                     VHRSIRLLTVMLLFFLAGARILNFIINPKAYGTNLHLYSLKFAGFSVYGGIFGALAAL
                     LIWCKIERTDPQPVLDA"
     gene            complement(368..>1611)
                     /locus_tag="PLU43_09470"
     CDS             complement(368..>1611)
                     /locus_tag="PLU43_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28683.1"
                     /translation="ATATYFESKASGVTPTIVSEYKNQIFTGVIPADNTSANGYARMP
                     LIAYMAGRNQETFIKSAYEMVKEGADWESALLSSMGPSENWAGEFYKELVSGNVGDYV
                     PYTIYSNLSKNQMAELGSFVALKVPSEDEIKKASEDGEVPELGKATLNIDGYGAKLLA
                     VTIDDTNLKQLPEDMNPSVQVSGNASVTIFRIKGKSFETFDGGNTEGIELADFVKSMQ
                     DKEVFLILVTGLHENGKQDYEVTISMPTYPTLDELVGTYSDGSVYYKEIHVADELKEK
                     MNEAAAETEEGGDEQYCDLNVLAMIEEMEGQTKPATLVIAKTGEDTGTLLLQYEGTDT
                     DSSDAIPFTYTNGTLIIDDIKTEESMNAVLSGNLKAAYGKNKDVCVNGSLYASFESAE
                     VSVKDGALLKIEIVVSGSKLL"
BASE COUNT          440 a          385 c          291 g          495 t
ORIGIN      
        1 gcatccaaaa caggctgagg gtctgttctc tcaattttgc accatatgag cagagctgcc
       61 agcgcaccaa aaattccgcc atacacagaa aatcctgcaa atttcaaaga ataaagatgc
      121 agatttgttc cgtatgcttt cggattgatg ataaaattca gaattctggc acctgctaaa
      181 aaaaacagca gcattacggt cagaagccgg atcgatctat gaacggaaat cccctccctt
      241 tttaacaaag ggagggacaa aaatattccg gtcaatgcac cgataaacgc aaataatgta
      301 taagaagcaa tcgtatattt ggatccggac aaggtaaatg taatccatgg atacatgaaa
      361 ttacctccta tagcagcttg cttccggaaa caacaatttc aattttcagc agggctccgt
      421 ccttcacaga tacttctgca gattcaaaac ttgcataaag cgaaccgtta acacagacat
      481 ctttattctt tccgtaagca gcctttaaat ttccggataa tacggcattc attgattctt
      541 ccgtttttat gtcatcaatg atcagagttc cgttcgtata agtaaatgga atcgcatcgg
      601 aggaatccgt atcagtccct tcatactgca gcagtaacgt tcccgtatct tctccggttt
      661 ttgcaatgac aagcgttgca ggcttcgtct gtccttccat ttcttcgatc atggcaagca
      721 cgttcaggtc gcagtattgt tcgtcaccgc cttcttctgt ttccgcagcc gcctcgttca
      781 ttttttcttt taattcatct gcaacatgaa tttccttata ataaaccgaa ccatccgaat
      841 aggttccgac cagttcatcc aatgtaggat atgtcggcat ggaaatggtg acttcataat
      901 cctgcttacc gttttcatgc agtcctgtga ctaatattaa aaagacctct ttatcctgca
      961 tcgattttac aaaatccgca agttcaatac cttccgtatt tcctccgtca aatgtttcaa
     1021 agctttttcc tttgatacgg aaaatcgtta cggaagcatt tccgcttacc tgaacacttg
     1081 gattcatatc ctcaggcagc tgtttcagat tggtgtcatc gatcgtaact gcaagaagct
     1141 tcgccccata cccgtcgatg ttaagcgttg cctttccaag ctccggcacc tctccgtctt
     1201 ctgatgcttt ttttatttca tcttctgaag gaactttcag tgcaacaaaa gagcccaatt
     1261 cagccatctg atttttggaa agattgctgt agatcgtata aggtacataa tcgcctacat
     1321 tgccggacac cagttcttta taaaattcac cggcccagtt ttcagagggt cccatggaag
     1381 ataataatgc agactcccaa tccgcgcctt cttttaccat ttcataggca cttttgataa
     1441 acgtttcctg atttctgcct gccatataag caatcagcgg catacgtgca tatccgtttg
     1501 ccgatgtatt atcagcggga attactcctg taaatatttg atttttatat tcgcttacga
     1561 tcgtaggagt tacacctgat gcttttgatt caaaatatgt tgcagttgcc t
//
LOCUS       DAOSYE010000583         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67377_length_1607_cov_2.786082, whole genome shotgun sequence.
ACCESSION   DAOSYE010000583 DAOSYE010000000
VERSION     DAOSYE010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67377_length_1607_cov_2.786082"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLU43_09475"
     CDS             complement(<1..704)
                     /locus_tag="PLU43_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOO28684.1"
                     /translation="MAIKGIDVSVWQGAIDFNAVRNSGVDFVIIRAGYGTNSKDKYFE
                     ENYRKAKAAELHVGAYWYSYADSFSEAVQEAEMFLSTLAGKQFDYPVFLDMEEKKQIE
                     AGTDFCSGLIKSFCDRLEAAGYFAGFYTSASFAGSVVTDAVRKRYCYWCAQWADACSY
                     EDSCGIWQHSSNGSVPGINGRVDMDWSYQDFPTVIIGGGFNGYSKSADDTPTPVASKT
                     VDELAHEVLAGNWGNG"
     gene            complement(685..1131)
                     /locus_tag="PLU43_09480"
     CDS             complement(685..1131)
                     /locus_tag="PLU43_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006289932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOO28685.1"
                     /translation="MKEFWNSLQLVLSAIGGWLGYFLGGCDGLLIALLIFVICDYITG
                     VMGAISDKKLSSAVGFRGICRKVLIFILVGIANVIDINVLGQVGVLRTAVIFFYISNE
                     GLSLIENAAHLGLPITGKLKEVLEQLHNRDEKDTDKEEKQHGYKRN"
     gene            complement(1210..>1607)
                     /locus_tag="PLU43_09485"
     CDS             complement(1210..>1607)
                     /locus_tag="PLU43_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28686.1"
                     /translation="YYNTVYSSNETFSGFKPYSDKTSDYAGAPDIVWTEGTLGYSLLA
                     LILGNKEEAQKYVDECIRLQNIDASTGGVIYTTATYGMLPWEFHVWESVVSSSWLYLI
                     INNPDILFPRTLRQVFYMTKITNMKDERP"
BASE COUNT          420 a          401 c          357 g          429 t
ORIGIN      
        1 tcgccgttgc cccagttccc agcgagcact tcatgcgcca gctcatcaac ggtttttgaa
       61 gcgacgggag taggagtatc gtcagctgat tttgagtatc cgttaaaccc gccgccaatg
      121 atgactgtcg ggaagtcctg atatgaccag tccatatcca cacggccatt gatgccggga
      181 acggagccat tggagctgtg ctgccagatt ccgcaggagt cttcatagct gcaggcatcg
      241 gcccactggg cgcaccagta acagtaacgc ttgcggacag cgtccgtcac aacagatccc
      301 gcgaaggatg cagaggtata aaatccggca aaatatccgg cagcctccag tctgtcgcag
      361 aaagacttaa tcaggccgga gcagaaatca gtcccggctt caatctgctt cttttcctcc
      421 atgtcgagga aaacaggata gtcaaactgc ttcccggcaa gtgtagacag gaacatttca
      481 gcttcctgta cggcttcaga gaagctgtct gcatagctgt accagtaagc gccgacgtga
      541 agttcggcag cttttgcctt cctgtagttt tcttcaaagt acttgtcttt tgagtttgta
      601 ccgtagcctg cgcggatgat cacgaaatcc actccgctgt tcctgacagc attaaaatca
      661 atcgcgccct gccataccga tacgtcaatt ccttttatag ccatgttgtt tttcctcctt
      721 atccgtgtct ttttcatcac ggttatgcag ctgctccagt acttccttca gctttcccgt
      781 aatcggaagt ccaaggtggg cagcattttc aatcagagac agaccttcgt ttgagatgta
      841 aaagaagatg actgccgtcc gcaggacacc gacctgtccg agtacattga tgtcgatgac
      901 atttgcgatg ccgaccagaa tgaagatcag taccttgcgg cagatgccgc gaaagccgac
      961 cgccgaggag agctttttgt cgctgatcgc acccatgacg ccggtgatgt agtcgcagat
     1021 gacaaagatc agcagtgcga taagcaatcc gtcgcaacca ccaaggaagt agccaagcca
     1081 gcctccgatt gcggacaaaa caagttgtaa cgagttccag aattctttca taataaattc
     1141 ctcctttgaa tttttgcatg aaaaaggcgg cctcacgtag gaagtcgcca tacatgaaaa
     1201 accatttttt tatggtcttt cgtctttcat atttgttatc tttgtcatat aaaacacttg
     1261 tcgaagtgtt ctcgggaata agatatctgg attgtttatg ataagataca gccatgatga
     1321 agatactacg ctttcccata catgaaactc ccaaggaagc attccgtaag tagctgtggt
     1381 ataaatcaca ccgcctgtgc tagcatctat gttctgaagt cgtatacatt catccacata
     1441 tttctgagct tcttctttat ttccaagaat aagagcaagt aatgaataac caagtgttcc
     1501 ttccgtccac acaatatcgg gtgcacctgc atagtccgac gttttgtcac tgtacggttt
     1561 aaatccagaa aatgtttcat tacttgaata aacagtgttg taataat
//
LOCUS       DAOSYE010000584         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67462_length_1606_cov_2.935525, whole genome shotgun sequence.
ACCESSION   DAOSYE010000584 DAOSYE010000000
VERSION     DAOSYE010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67462_length_1606_cov_2.935525"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLU43_09490"
     CDS             <1..902
                     /locus_tag="PLU43_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO28687.1"
                     /translation="RFIMMNMDGKTVEKPAGDALAPVDRWILSKLNRVVLEVTDHMEH
                     YELGIAVQKVYDFIWDEFCDWYIEMVKPRLYNSDDTISQNAALYTLREVLIDALKLLH
                     PYMPFITEEIFCTLQNAEESIMISKWPVYTKEREFPEEEKAIETIKEAVRGIRNARAD
                     MNVPPGRKAHVFVVSDDVQIRKIFEDGKLFFSSLAYASLVTVQSDRSGIAEDAVSVVI
                     PGASVFIPFAELVDIKQEIERLEKEEERLKKELARSNGMLNNSKFISKAPAEKIREEK
                     EKFEKYTRMTQEVKERLTQLKRL"
     gene            1011..>1606
                     /locus_tag="PLU43_09495"
     CDS             1011..>1606
                     /locus_tag="PLU43_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOO28688.1"
                     /translation="MVNFDTDCRVIIADDNMSAKVYLTPPTPGNPYTVEDIADFLRHN
                     GVNGGIIFSVLESIIRDGLYYYEIEVAKGSEPVHGQNGWYEFFFNQEEIKHPLIRSDG
                     SVDYQSMSIIENISIGDVLAVYHPAVQGSHGMDVKGRDFRAKPGKELPQLRGAGFERS
                     YDGNTYTATIEGKVEYDNFKLYIRDVYEFRGDLDLVVG"
BASE COUNT          460 a          296 c          438 g          412 t
ORIGIN      
        1 cccgcttcat catgatgaat atggatggaa agactgtcga aaaaccggca ggggatgcgc
       61 ttgcccctgt tgaccggtgg atcctgtcaa agctcaatcg ggtcgtttta gaagtgaccg
      121 accatatgga gcattacgag cttgggatcg cggtccagaa ggtctatgat tttatatggg
      181 atgaattttg cgactggtat attgagatgg tgaaaccaag actgtataac agcgacgata
      241 caatctcaca gaatgcagcc ctttatacgc tccgggaagt tctgatcgat gcattaaagc
      301 tgctgcaccc gtatatgccg tttatcacgg aagagatctt ttgtacgctg caaaatgcgg
      361 aagaatcgat catgatctct aagtggccgg tatatacgaa ggagcgggaa tttccggaag
      421 aggaaaaagc gatcgaaacg atcaaggaag ctgtcagggg cattcgtaat gcgcgtgcgg
      481 acatgaatgt gccgcctggg aggaaagccc atgtgtttgt cgtatccgac gacgtacaga
      541 tcaggaagat ctttgaggac ggaaaattgt ttttttcatc gcttgcctac gcatctttgg
      601 taacggttca aagtgacaga agcggtattg cagaggatgc ggtatcagtt gtaattccgg
      661 gagcatccgt ctttatccca tttgcggagc tggtcgatat caaacaggag atcgagcgtc
      721 ttgaaaagga agaggaacgg ctgaaaaaag aactggcgcg ttcaaacggt atgctgaaca
      781 acagtaaatt tataagcaag gcgcctgctg aaaagatcag ggaagaaaag gaaaagtttg
      841 agaaatacac gcggatgaca caggaagtga aggaacgtct cacgcagctg aaaagacttt
      901 aattatttat gaaaaacacc cgtgatacat tgcacgggtg ttttgtaata tgatataata
      961 ttgacagata gaaaactgct gttgttattt gattcaacgg ggaaattcat atggttaatt
     1021 ttgataccga ctgtcgtgtg atcatcgccg acgataatat gagtgcaaag gtttatctga
     1081 cgccgcccac tccgggcaat ccgtatacgg ttgaagatat tgctgatttt ctgcgtcaca
     1141 atggtgtgaa cggaggaatt attttttctg ttctggaaag cattattcgg gatggcttgt
     1201 attattatga gatcgaggtg gccaaaggca gtgaaccggt acatggtcag aatggctggt
     1261 atgagttctt tttcaaccag gaggagatca agcatccgct gatccgttcc gacggttccg
     1321 tggattatca gagcatgagc attattgaaa atatttcgat cggagatgtc cttgcagttt
     1381 atcatccggc ggtgcagggt tcgcacggga tggatgtaaa aggaagggat tttcgggcca
     1441 agcccggaaa agaactgcct cagctgcgcg gcgcgggctt tgaaagatct tatgacggca
     1501 atacatatac cgcaacgata gagggaaaag tagaatacga taattttaag ctgtatatca
     1561 gggatgttta tgaattcaga ggtgatctgg accttgttgt cggcaa
//
LOCUS       DAOSYE010000585         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67526_length_1605_cov_3.243871, whole genome shotgun sequence.
ACCESSION   DAOSYE010000585 DAOSYE010000000
VERSION     DAOSYE010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67526_length_1605_cov_3.243871"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLU43_09500"
     CDS             complement(<1..1089)
                     /locus_tag="PLU43_09500"
                     /inference="COORDINATES: protein motif:HMM:NF022927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HOO28689.1"
                     /translation="MDKAFFEVFPTLKLNKNLQELFEETFVTRVVSSSRKDRLRIYLS
                     CRCLIEKDVIYTVEREIVRQLFPKAKIKVTFMESFHLTGRYTTEGLLSVYKNSILLEL
                     KNYDHFEYQLFYHAELEFPEDTVLKLVLEDTIVARSRSEELVRILDKILNERCGISTE
                     VYVSYREAAPKKARKNAELKMQEEVSRIVQMSKDGAEAFKEGEPEQQPAEERTTDAPE
                     PVKAVNEKNKFEKKNWPSRRGSRIKKSDNPDVIYGYDFNEEPVVISEIDGEIGEIIVK
                     GQIIRFETRDTRNEKKLVIFDVTDFTDTITVKMFPKKEHWEKLSADIGEGKFVIVKGV
                     INVDKFDGELTLGFVTGIKKTEDFTSSRM"
     gene            complement(1138..>1302)
                     /locus_tag="PLU43_09505"
     CDS             complement(1138..>1302)
                     /locus_tag="PLU43_09505"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO28690.1"
                     /translation="CVVNGPGEAKEADIGIAGGKGEGLLIKKGEIIRKIPENELLSTL
                     KMELDNWNLQ"
     assembly_gap    1303..1312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1313..>1605)
                     /locus_tag="PLU43_09510"
     CDS             complement(<1313..>1605)
                     /locus_tag="PLU43_09510"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO28691.1"
                     /translation="LHVGITEAGTVKSGNIKSAAGLGIILYQGIGDTIRVSLTGDPVE
                     EVVSAKLILRTLGLRKGGIEVVSCPTCGRTNIDLISLASEVEKLAAGYDLAIKV"
BASE COUNT          412 a          422 c          260 g          501 t
ORIGIN      
        1 cattctggaa gatgtaaaat cctcagtttt tttaataccg gtgacaaacc caagcgtcag
       61 ctccccgtca aatttatcaa cattgatcac acctttaaca atgacaaatt tcccttcccc
      121 gatatcagca gacaattttt cccaatgttc ttttttagga aacatcttca ctgttatcgt
      181 gtctgtaaaa tccgtaacat caaatataac cagttttttc tcgtttctcg tatctcttgt
      241 ctcaaaccgg ataatctgcc ctttgacaat gatctctccg atctcaccgt caatttcaga
      301 aatgacgaca ggttcttcat taaaatcata tccatatatg acatccggat tatctgattt
      361 cttaatacgg gaacctctgc gggaaggcca gttctttttt tcgaatttgt ttttttcatt
      421 tacggctttt acgggttccg gagcatctgt tgtcctctct tcagccggct gctgttccgg
      481 ttctccttct ttaaacgcct ccgccccgtc tttagacatc tgaacgattc tgctgacttc
      541 ctcctgcatt ttcagctccg cattttttct tgcttttttt ggcgcagctt ccctgtagga
      601 aacataaacc tccgtactga tcccgcagcg ctcgtttaaa atcttatcca gaatacgaac
      661 aagctcctcc gaacggcttc ttgcaacgat cgtgtcttca agcacaagct ttaatacagt
      721 atcctccgga aattccagtt ccgcatgata gaacagctga tattcaaaat gatcataatt
      781 tttcaattcc agaagaatac tgtttttata tactgaaaga agcccttcag tcgtatagcg
      841 gcctgtcaga tgaaaacttt ccataaacgt aactttgatc tttgctttcg gaaacaactg
      901 acgtacaatt tcacgttcca cagtgtaaat aacatccttt tcgataaggc atctgcagct
      961 taaataaatc ctcagccggt ctttgcggct tgaagatacg actcttgtaa caaaagtctc
     1021 ctcgaacagt tcctgcagat ttttattcaa ttttaatgtg ggaaatacct cgaaaaatgc
     1081 tttatccatt tcatccaccc gatcatttat tccgtatcag tccgcaataa agctctatta
     1141 ctgcaggttc cagttgtcca gttccatttt tagtgtggaa agcagttcat tttccggaat
     1201 ctttcggata atctcaccct ttttaatcag cagaccctct ccttttccgc ctgcaatacc
     1261 gatatcagcc tcttttgcct ctcctggccc attcacaaca cannnnnnnn nnaccttgat
     1321 agcaaggtca taacctgccg caagcttttc aacttctgat gcaagtgaga tcaaatcaat
     1381 attcgtcctg ccgcacgtcg gacaggaaac aacctcaata ccgccttttc taagtccgag
     1441 cgtacgcagg atcagcttgg cggaaacgac ttcctcaacc ggatcccctg tcagggatac
     1501 acgaatcgta tcaccgatac cctgatataa aataattccc agtcctgcgg ctgacttaat
     1561 attccctgat ttcacggtac cggcctccgt aatgcccacg tgcag
//
LOCUS       DAOSYE010000586         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67547_length_1605_cov_2.675484, whole genome shotgun sequence.
ACCESSION   DAOSYE010000586 DAOSYE010000000
VERSION     DAOSYE010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67547_length_1605_cov_2.675484"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLU43_09515"
     CDS             complement(<1..687)
                     /locus_tag="PLU43_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO28692.1"
                     /translation="MTHSEFRQLAENIIYLDGATGSNLVKRGMPAGVCPERWILDHPE
                     VMIGLQEEYLEAGTNILLAPTFTSNRIKLAEYGLEDREEELNRRLVGLSQTAIARYRE
                     KIGKTDPRSRHLYVAADITMTGQQLKPMGTLDFEDLVDIYKEQLTYLVSAGVDLVIIE
                     TMMSLQETRAALLAAKETCDLPVIASLTYENDGRTLYGTDAMTAMAVLQSLGAAAVGT
                     NCSTGPDKMLF"
     gene            complement(894..1340)
                     /locus_tag="PLU43_09520"
     CDS             complement(894..1340)
                     /locus_tag="PLU43_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOO28693.1"
                     /translation="MMNLSNVSEEYGIYGMLFSLGNRIQTIGDKEFEDITMKQHFMMI
                     ALGMFEQPPTLKEMGTVIGCSYQNVKRMAEHLQDSHYLNMEKDSLDKRKLRLKSTGRL
                     EKMADEQRDRTIEFMEMLYKNIPREDLAATLRTLMKMDRNIGGVIE"
BASE COUNT          425 a          397 c          272 g          511 t
ORIGIN      
        1 gaacagcatc ttgtcaggtc ctgtggaaca attcgtacca actgctgctg ctccaagact
       61 ctgcagcacg gccattgccg tcatcgcatc tgttccataa agcgtacgtc catcattttc
      121 ataggtcaaa gaagcaatga ccggcagatc acaggtctcc ttcgccgcaa gcagtgcagc
      181 tcttgtttcc tgcagactca tcattgtttc tataataaca agatcgacac ctgcactgac
      241 aagatacgtc agctgctcct tatagatatc gaccagatct tcaaaatcaa gtgttcccat
      301 gggctttaac tgctgaccgg tcattgtaat atcagccgcc acataaagat gccggctgcg
      361 cggatctgtc tttccgattt tttctctgta ccgcgcaatg gctgtttgtg aaagacccac
      421 aagccttctg ttcaattcct cttccctgtc ttccagcccg tattctgcca gtttgatccg
      481 gttagaagta aaggtcggcg ccagcagaat atttgttcct gcttccagat attcttcctg
      541 aagaccgatc atgacctcag gatgatccag gatccagcgt tccggacata cgccggccgg
      601 cattcccctc tttaccagat tggaacccgt cgctccgtcc agataaataa tattttccgc
      661 cagttgtctg aattcactat gagtcattgc attcactcca tcatcaaaga ttctttaaac
      721 tttcccgtaa agtatattat accgaaaagt aatataaaat gaaaggaaaa aaaagaaaat
      781 ccgaagcagg catggatgct gtctgtttcg gatcatcgct caaagcgtgc tttccgcgta
      841 ttttttcgtg aatccggtag cgcttttata gatcactatt ttctttatct ttgtcattca
      901 atcacacctc ctatattccg atccattttc atcaatgttc ttaaagtagc agctaagtct
      961 tcccttggaa tatttttata tagcatctcc ataaattcaa ttgttctgtc tctttgttca
     1021 tctgccattt tttccagcct gccggtagac ttaaggcgca gttttctttt atccaggctg
     1081 tctttttcca tatttaaata atggctgtcc tgtaaatgct ctgccatcct tttcacattc
     1141 tgataagaac acccgatcac tgttcccatt tctttcaggg taggaggctg ttcaaacatt
     1201 cccaatgcga tcatcataaa atgctgtttc attgtaatat cttcaaattc tttatcaccg
     1261 atcgtctgga tcctgttacc aagcgagaac aacattccat agatcccgta ttcttcactt
     1321 acatttgaca gattcatcat tgatccccct tatatacata acatgctatg taattaatat
     1381 ataacatgtt atgtaatttg taaatacaaa aaaggctgcc agactaaaat ataatctgaa
     1441 cagcctgctt tttatgattc aactccaaat gaagggaatt ctgcctgcac tttttctgta
     1501 aaaccatctt cgatcatggt tatcataatg gaggcggcaa caggatcaaa ttgttttcct
     1561 gaattttcat tcagctcctg caaaataaca tccctgctca atttg
//
LOCUS       DAOSYE010000587         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67601_length_1604_cov_3.377663, whole genome shotgun sequence.
ACCESSION   DAOSYE010000587 DAOSYE010000000
VERSION     DAOSYE010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67601_length_1604_cov_3.377663"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLU43_09525"
     CDS             <1..221
                     /locus_tag="PLU43_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO28694.1"
                     /translation="LLGMKAFAAAILGGIGSIPGAVMGGFVIGIIESLGAGYLSSDYR
                     DAYAFIIMILILILRPSGIMGAKHIDKV"
     gene            237..914
                     /locus_tag="PLU43_09530"
     CDS             237..914
                     /locus_tag="PLU43_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO28695.1"
                     /translation="MSKKNGFFKKLSEIKLTQKQKTIALIICMLLLIVFPFIVTNKYM
                     LHIIILCFMYACLTLSLNLIIGWSGQFSLGHVCFYGMGAYITTLLMMRFEVNFFLATL
                     ISIICTSIFSALLCYPTLRLRGDYIAVVTLGFGEVFRLFLTNAVDLTRGPAGIPGISK
                     PEIFGLVIKGKTAYYFFALILLIIFVGFMNAASCQCDKYSRFLRRGGQCTYDRSDDRS
                     CTQDHVL"
     gene            823..1530
                     /locus_tag="PLU43_09535"
     CDS             823..1530
                     /locus_tag="PLU43_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO28696.1"
                     /translation="MTNIPGFCAEEVSVHTIGLMIDLVRKITYFDKKVREGKWDPLIG
                     YKTYRMTGMTFGMVFFGEIPKKMVPVLKALGLKILVYAPTKTTEFLAEYGCEKAETLD
                     ELFAASDFVSLHCPLIPDVTWHLIGEEELKKMKPTAFLINTARGSVVDEPALVKALKE
                     GWIKGAGIDVIEDETNEVSELFQFNDNVVITPHAAFISEDSFYDGRKRALWQLVMRFS
                     KKERPSSLVNKTVTIDL"
BASE COUNT          438 a          299 c          391 g          476 t
ORIGIN      
        1 atctgcttgg tatgaaagca tttgcagcag caatcctcgg aggcattggc tcgattccgg
       61 gtgcagttat gggaggcttt gtaattggta tcattgaaag cctgggcgca ggatatcttt
      121 cctcggacta cagagacgcc tatgcattta tcataatgat tctgattctt attttaagac
      181 cgtcagggat tatgggtgca aagcatattg ataaagtata ggaagaggtg atacggatga
      241 gtaagaaaaa cggttttttt aaaaagcttt cggaaattaa gcttacacaa aaacagaaga
      301 cgatcgcact gattatctgt atgcttcttc tgattgtatt tccatttatt gtgacaaaca
      361 aatatatgct gcatattatc attttatgtt tcatgtatgc atgcctgaca ctttccctga
      421 atttgatcat tggctggtcc gggcagtttt cacttggaca tgtgtgtttt tatggaatgg
      481 gtgcttatat tacaacgctt ttgatgatga gatttgaagt caatttcttt ttggcgacac
      541 tgatcagtat catctgcacg tccatattca gtgcgcttct ttgttatccg acgctccgtc
      601 ttcgcggtga ctatatcgca gtggtgactt tgggattcgg cgaagtattc cgcctgtttc
      661 ttaccaatgc ggtcgatctg acaagaggcc ctgctggaat ccccggaatt tccaagccgg
      721 agatctttgg acttgttatt aaaggaaaga cggcatatta cttttttgca ttgattttat
      781 tgattatttt tgtcggattt atgaacgcag catcatgtca gtgtgacaaa tattcccggt
      841 ttctgcgcag aggaggtcag tgtacatacg atcggtctga tgatcgatct tgtacgcaag
      901 atcacgtact ttgacaaaaa ggtcagggaa gggaaatggg atccgctgat cggttataaa
      961 acttaccgga tgaccggcat gacgtttggc atggtatttt tcggagagat tccgaaaaaa
     1021 atggtgccgg ttttaaaagc gcttggactg aagattcttg tttacgcacc gacaaagacg
     1081 acggaatttc ttgcggaata cggctgtgaa aaagcagaga cactcgatga actgtttgcg
     1141 gcatctgatt ttgtatcact gcactgtccg ttgattccgg atgtgacatg gcatctgatc
     1201 ggagaagagg aactgaagaa gatgaaaccg accgctttcc tgatcaatac ggcacgcggt
     1261 tccgttgtgg atgaaccggc acttgtaaag gcactgaagg aaggctggat caaaggagcc
     1321 ggtatcgatg tgatcgagga tgagacaaat gaagtcagcg aactgttcca gtttaacgac
     1381 aatgtcgtga tcacgccgca tgctgcattt atttcggagg attcattcta tgacggcaga
     1441 aagcgtgcac tgtggcagct tgtcatgcgg ttttccaaga aggaaagacc gtcaagcctt
     1501 gtaaataaaa cagtaacaat tgatctttaa taatacacaa tatgatttca ggagcttcct
     1561 gtatacttta aagagtgaaa ggaattgacc aaaaagaata cctc
//
LOCUS       DAOSYE010000588         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_67876_length_1600_cov_3.370227, whole genome shotgun sequence.
ACCESSION   DAOSYE010000588 DAOSYE010000000
VERSION     DAOSYE010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67876_length_1600_cov_3.370227"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..534)
                     /locus_tag="PLU43_09540"
                     /pseudo
     CDS             complement(34..534)
                     /locus_tag="PLU43_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
     gene            complement(682..>1600)
                     /locus_tag="PLU43_09545"
     CDS             complement(682..>1600)
                     /locus_tag="PLU43_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO28697.1"
                     /translation="GQMKEHELERIMFDFVNGEIDVLVSTTIIETGLDISNVNTMIVH
                     DSDHMGLSQLYQLRGRVGRSNRTAYAFLMYKKNKLLKETAEKRLQAIREFTELGSGFK
                     IAMRDLEIRGAGNLLGERQSGHMESVGYDLYCKMLNEAVKQKRGIETVEDYSVGVDLN
                     VDAYIPDTYIVNEYQKIDIYKRIASIENMTEKEDIQEELTDRFGSLPKSVLNLLEIAY
                     LRVLAKTVFITEIKGENKAVKLSIYEKAEYNTEKIGSFVAGYKGTLSLRAVAKPYFIY
                     SLPKKTPDDKEVVLEKTKELLVQMKALLF"
BASE COUNT          416 a          423 c          278 g          483 t
ORIGIN      
        1 cagagggaag tttctgattt ctcagaggtt ttctgttctt gtaacaaaat cctctacctg
       61 atccggctgt gtataagact ctgaaccatg ttccaccttt acgtcatcct caacgcccgc
      121 aagcgtacca agctctgcct caactacaac acctttatca tgtgcgtatt cgactacctt
      181 ctttgcaatc ttaatatttt cttcataagg aagcgaagaa ccatcataca ttacggaagt
      241 aaacccgccg tcgatacagg atttgcagat ctcaaaatcc gcaccatgat ccagatgaag
      301 agcaatcgga atatcggtct cctcaagagc agcctgtaca agatgtacca gataggaatg
      361 ctttgcgtac tttctggccc ccgcggaaca ctggagaata ataggggatt tcaattcatc
      421 cgccgcctcc gtaatagcct gtacgatctc catgttgttt acattgaaag caccgattgc
      481 atatccgcct tcataagcct tcttaaacat ttccgttgtt gtaactaatg ccattgttta
      541 tcatcctttc cttttggaat taaaatatac tatacagagc agtactttac agaaagtaac
      601 tgcttacctg gattatagca ccaaattctg caaacgacaa ttatttctcc tatactttac
      661 aaacttttta tatcattttc cctaaaacag aagcgccttc atctgcacaa gaagttcctt
      721 tgttttttcc agaacaactt ctttgtcatc cggcgttttt tttggcagac tataaataaa
      781 atatggcttg gccactgccc gcagactcag ggttccctta tacccggcaa cgaacgaacc
      841 gattttttcc gtattatatt ccgctttttc gtaaatgctc agtttgacgg ctttattttc
      901 ccctttgatt tccgtaataa atacggtttt tgccagcaca cgcaaataag cgatctccaa
      961 aagattcaga acagactttg gcagactgcc gaaccgatcc gtcagttcct cctgaatatc
     1021 ttccttttcc gtcatgtttt caatactggc aatcctctta taaatatcta ttttctgata
     1081 ttcgtttacg atataggtat ccggaatata cgcatccaca ttcagatcca caccaacgga
     1141 ataatcttcc acagtctcga tcccccgttt ttgcttgact gcttcattca gcattttaca
     1201 gtacagatca tagccgacag attccatgtg accgctctgc cgctcaccga gcagatttcc
     1261 ggcacccctg atctcaaggt cccgcatcgc gatcttaaag ccgctgccaa gctcggtaaa
     1321 ttcccggatc gcctgcagcc gcttctcagc cgtttccttg agcagcttgt tttttttgta
     1381 catcaaaaag gcgtatgcgg tcctgttcga cctgccgaca cgtccccgca gctggtagag
     1441 ctgggaaaga cccatgtggt cggaatcatg gacgatcatc gtattcacat tggaaatatc
     1501 cagacctgtc tctatgatcg ttgttgaaac aagcacatcg atttccccgt ttacaaaatc
     1561 aaacataatt cgttccagct catgttcctt catctgccca
//
LOCUS       DAOSYE010000589         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68158_length_1596_cov_2.778715, whole genome shotgun sequence.
ACCESSION   DAOSYE010000589 DAOSYE010000000
VERSION     DAOSYE010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68158_length_1596_cov_2.778715"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLU43_09550"
     CDS             <1..551
                     /locus_tag="PLU43_09550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOO28698.1"
                     /translation="GIYITRPDAGNLSNIISCMVAMDHIGYIDIYDVRIILEESAAEI
                     AATCVTDDEIDRMETLLQHLDNTMLSVKERREADFQFHIEIAKATKNMLLVILIQTMK
                     NIFIDMIETGIFLQGGIADAAARHRKILDALKAHDPVLARQSMHEHLLVSRENTKKVY
                     LDREKAKLLNKKHDGKTEEANC"
     gene            581..1075
                     /locus_tag="PLU43_09555"
     CDS             581..1075
                     /locus_tag="PLU43_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO28699.1"
                     /translation="MKETLKKMNDNFEKWIMMLLFGVLFMIVMAGIISRCVFNQPFTW
                     TEEAARLVFIWLVFMGISYGTKYDKHINVTIIMDRMPKIAAALLTVFWDIVALVIFLW
                     ITFYGTKYILYMAGSYTSVLRIDQSITTSIVAISAVLNCIRILENLITKHLPEVKKCK
                     EETV"
     gene            1072..>1596
                     /locus_tag="PLU43_09560"
     CDS             1072..>1596
                     /locus_tag="PLU43_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO28700.1"
                     /translation="MSTAFIVFLLVFVIFLFFGAPIYLSLLCSGLAYVMIHGAIDNMA
                     VMAKMYASLNNFVLLAIPMFMMAGIIMNQGGITNRIFGFCRSLLGHFPGGLAYVNIFS
                     SFIFSGMSGSALADVGGLGQVEIKAMRDEGYEDDLTFGITAASATMGPIVPPSIPMVI
                     YGAAASVSVGTLFMA"
BASE COUNT          465 a          269 c          387 g          475 t
ORIGIN      
        1 caggcatata tatcacaagg ccggatgccg gaaacctgtc aaatattatc agctgtatgg
       61 tggcaatgga tcatatcgga tatattgata tttatgatgt tcgtatcatt ctggaagaat
      121 ctgcggctga aatagcagcg acctgcgtga cggatgatga gatcgacaga atggaaacgc
      181 ttctgcagca tttggataat acgatgcttt ctgtaaagga acgaagggag gcagattttc
      241 agtttcatat agaaattgca aaagcaacaa aaaatatgct tctggttatt ctgatccaga
      301 caatgaaaaa tatctttatc gacatgattg aaacaggaat ttttctgcag ggaggaattg
      361 cggatgcggc tgccagacat agaaaaattc tggatgcatt gaaagcacat gatccggttc
      421 tggcaaggca aagcatgcat gaacatctgc ttgtgtccag agagaatacg aaaaaagtat
      481 atctggacag agaaaaggca aaactgttaa ataaaaaaca tgacggcaaa acggaagagg
      541 caaactgctg atgagcaggg accaattttg ggagggtaca atgaaggaaa cattaaaaaa
      601 aatgaatgac aattttgaaa aatggattat gatgctgtta tttggtgtgc tatttatgat
      661 tgttatggcg ggaatcatct ccaggtgcgt atttaaccag ccgtttacct ggacggagga
      721 agcggcccgc cttgttttta tctggcttgt ttttatgggc atttcctatg gaacaaaata
      781 tgacaagcat atcaatgtta cgatcataat ggacagaatg cctaaaatag cagcagcatt
      841 acttactgta ttttgggata ttgtggcgct ggtcatattt ctatggatta ctttctatgg
      901 aacaaaatat atattatata tggcaggttc ctatacttct gttttgcgaa tcgatcagtc
      961 gatcacgact tcgattgtag cgatcagtgc agttctgaac tgcatcagaa ttttggagaa
     1021 tctgatcacc aaacatctgc cggaagtaaa aaagtgtaag gaggaaactg tatgagcacg
     1081 gcatttattg tttttttact tgtatttgta atatttcttt tttttggagc accgatttat
     1141 ctgagtcttt tatgcagtgg tttggcgtat gtaatgatac atggcgcgat cgataacatg
     1201 gcggtcatgg ctaagatgta tgctagcctg aacaattttg tactgctggc aattccgatg
     1261 tttatgatgg cagggatcat tatgaatcag ggagggatca caaatcggat ctttggattc
     1321 tgccgctctc ttttgggaca ttttccagga ggccttgctt atgtcaatat tttttcctct
     1381 tttatttttt cagggatgtc cggttcggca ctggctgatg taggaggact tggacaggtg
     1441 gaaattaaag caatgcgtga cgaaggatat gaggatgatc tgacattcgg cattacagca
     1501 gcgtctgcca cgatgggacc aattgtaccg ccatcgatcc caatggtcat ttatggcgcg
     1561 gcggccagtg tttccgtagg tactcttttt atggca
//
LOCUS       DAOSYE010000590         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68369_length_1593_cov_3.719766, whole genome shotgun sequence.
ACCESSION   DAOSYE010000590 DAOSYE010000000
VERSION     DAOSYE010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68369_length_1593_cov_3.719766"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLU43_09565"
     CDS             <1..238
                     /locus_tag="PLU43_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOO28701.1"
                     /translation="EGKPPYFLIHKRQEEYGFSLIGYENEVIFSSVFYQEKSACTEAI
                     RELKDYAQTAVISDMTRTVHTQKNAGNCGKEGLY"
     gene            235..1149
                     /locus_tag="PLU43_09570"
     CDS             235..1149
                     /locus_tag="PLU43_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28702.1"
                     /translation="MNTKQIYKKTMPFVWAKLLIGLITVVLSAVLFALFIGLSWLFNS
                     GGVTLILFILWLFAVGFIRFAVMHYMGYLIKAGHIAVITEAVTTGKVPDNQIEYGKTV
                     VKERFATANVYFAVDKLVTGAVKQIQHGIEKVGNALDFVPGMDTVTSLAKLFVDLSLG
                     YIDECCLGYTFYKSEDSAFKSAADGVVIYAQNWKRLLKNAAKTMIMVILMLAAVTVLC
                     FLVLGILFRLLHWSGSVAFIISVLIAFAVKFAFVDSYILIKTMVSYMEVAPDTQITFD
                     LYQKLSDISGKFKELLKKGQEKRTGNSI"
     gene            1165..>1593
                     /locus_tag="PLU43_09575"
     CDS             1165..>1593
                     /locus_tag="PLU43_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO28703.1"
                     /translation="MAGIFDSFVREIVDLAPAEAPGIKRIKVKKELEELKEKENALYM
                     ELGRLAYEDGGKEKYPDIAVQLEGLTAAENEIQMKLQKAEEEEAKQDELESSRECPNC
                     GNFNPMEKKFCQTCGFKFPVQLQCPSCGADVAEGVRFCGEC"
BASE COUNT          484 a          250 c          429 g          430 t
ORIGIN      
        1 agaagggaaa ccgccatatt ttcttattca caagagacag gaggaatatg ggttttctct
       61 gattggatat gaaaatgaag tgattttcag cagtgttttt tatcaggaaa aaagtgcatg
      121 tacggaagcg atccgagaac tgaaggatta tgcgcagacg gcggtcattt cggacatgac
      181 gagaaccgtt cacacacaaa aaaatgcagg taattgcggg aaagagggat tgtattgaat
      241 acgaaacaaa tttacaaaaa aaccatgcct tttgtatggg caaagctatt gatcggactg
      301 attactgtgg tgctttcggc agttttgttt gcactgttca tcggccttag ctggctgttt
      361 aacagcgggg gagttacgct gatcttattt attctctggc tttttgcagt cggatttatc
      421 aggtttgccg taatgcatta catgggatat ttaattaaag caggacatat tgctgtaatt
      481 acggaggcgg taacgacagg gaaggttccg gataatcaga tcgaatacgg gaagactgtc
      541 gtaaaagagc gttttgcaac agcgaatgtt tattttgccg tagataagct tgtgaccgga
      601 gcggtgaaac agatccagca cggaatagaa aaggtcggaa atgcattgga tttcgtaccg
      661 gggatggata ctgttacaag cctggcaaaa ttgtttgtgg atctttcgct tggatatatc
      721 gatgaatgct gtcttgggta tacattttat aaaagcgaag acagtgcttt taagagtgcg
      781 gcagacggcg tcgttattta tgcgcagaac tggaaacgtt tattgaaaaa tgcggcgaaa
      841 acaatgatta tggtgatttt gatgctggcg gcagtgacgg ttctttgttt tcttgtgctt
      901 ggcatactgt tccgtctgct tcattggagc gggtcggttg cttttatcat atcggttttg
      961 attgcgtttg cagtcaaatt tgcttttgtt gacagttata ttctgatcaa gacaatggtt
     1021 tcctatatgg aagtcgctcc ggatacgcag atcacatttg atctatatca gaaattgagc
     1081 gatatttcgg gaaaattcaa ggaattattg aaaaagggac aggaaaagag aactggaaat
     1141 tcaatttaga aaggaagagg agatatggca ggtatttttg acagttttgt aagagagatc
     1201 gtggatctgg cgccagcaga ggcaccggga atcaaacgga taaaggtaaa aaaagaactg
     1261 gaagagctga aagaaaaaga aaatgcgctt tatatggagc tgggcaggct ggcatatgaa
     1321 gacggcggaa aagaaaaata tccggatatt gcggtacagc tggaaggact gacagcagca
     1381 gaaaatgaaa tccagatgaa attgcagaaa gcagaggaag aagaggcaaa acaggatgaa
     1441 ttggaatcaa gccgggaatg tccgaattgc ggaaatttta atcccatgga aaagaaattc
     1501 tgccagacat gcggattcaa attcccggta caattacaat gtccttcctg cggcgctgat
     1561 gtcgcagagg gagtgcgttt ctgcggtgaa tgc
//
LOCUS       DAOSYE010000591         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68459_length_1592_cov_2.890046, whole genome shotgun sequence.
ACCESSION   DAOSYE010000591 DAOSYE010000000
VERSION     DAOSYE010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68459_length_1592_cov_2.890046"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLU43_09580"
     CDS             <1..579
                     /locus_tag="PLU43_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO28704.1"
                     /translation="YIFGKSSTGKDTIYRKIAEDTTLDLKNIVMYTTRPIRDGEQDGN
                     TYYFVTENRFEELKEAGKIIEDRAYHTVHGLWRYFTVDDGQIDLENNDYFIIGVLQSY
                     LSTKAYFGKDKVVPIYIELDDGIRLQRALDREKKPENRKFAEMCRRYLADAEDFSEEK
                     LIKADIGKRFINEDLEKCVSEIKQFIRTDDLQ"
     gene            624..>1020
                     /locus_tag="PLU43_09585"
     CDS             624..>1020
                     /locus_tag="PLU43_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOO28705.1"
                     /translation="MDIKVNQTVPAAASAEQPQQTPGSDEAFRFTLMSHIEESGLQER
                     LTLLFEDITMQGKKISKHMDVKDMKKYRSLVKEFLNEIVNRSHKFSRENFLDRKGRHR
                     VYGIIRLVDQNLDALAEELMKEEKDHLAIL"
     assembly_gap    1021..1030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1103..>1592
                     /locus_tag="PLU43_09590"
     CDS             1103..>1592
                     /locus_tag="PLU43_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO28706.1"
                     /translation="MAGFNDIIGQEHLKEHLQKAIKTGNLSHAYMISGEKGSGKEFVA
                     NIFAMTLQCEKGGVEPCMECHSCRQAMTKNQPDIITVTHEKPNTISVEDIREQIVGDV
                     AVKPYASKWKLYIIKDGEKMSVQAQNALLKTLEEPPSYVIILILTANSSMLLETIRSR
                     CTE"
BASE COUNT          536 a          276 c          413 g          357 t
ORIGIN      
        1 tacatctttg ggaaaagttc cacgggaaaa gataccatat atagaaagat agcagaggat
       61 acgacacttg acctgaaaaa cattgtaatg tatacgacgc gtccgatcag ggacggggag
      121 caggatggaa acacgtatta ttttgtaacg gagaaccggt ttgaagaact gaaggaagcc
      181 ggtaaaataa ttgaagacag ggcatatcat acggttcacg gtctctggag atattttact
      241 gtcgacgacg gccagatcga tctggaaaat aatgattatt tcattattgg agttctgcag
      301 tcctatttat cgacgaaagc gtattttgga aaagataaag tcgttccgat ctatattgaa
      361 ctggatgatg gaattcgtct gcaaagagca cttgacaggg aaaagaaacc ggagaaccgg
      421 aaatttgcag aaatgtgcag aagatatctg gcagatgcgg aggatttttc cgaagaaaaa
      481 ctgataaaag cagatatcgg gaaacggttt ataaatgaag atctggaaaa atgtgtttcg
      541 gaaatcaaac aatttatcag aactgacgat ctgcaataac actgccggat agaattggtc
      601 aggaagagaa ggaggaaggg tctatggata tcaaagtaaa tcaaacggtg ccggcggccg
      661 cctctgccga acagccgcag caaacgccgg gctcagacga ggcgtttcgc tttacgctga
      721 tgagtcatat agaagaaagc ggcttgcagg aaagactgac attgcttttt gaggacatta
      781 caatgcaggg gaaaaagatt tcaaagcata tggatgtcaa ggatatgaaa aaatacagga
      841 gccttgtgaa agagtttctg aatgagatcg tgaaccgttc tcataaattt tccagggaaa
      901 atttcctgga cagaaaaggc aggcacaggg tatacggaat tatccggctg gtcgatcaga
      961 atctggatgc gctggcggaa gagctgatga aagaagagaa ggatcatctg gcaattctca
     1021 nnnnnnnnnn caataaagta ggtgagatca gaggcttact gctggatatt ctgacgtagc
     1081 cggaacagaa aggaaaaatt taatggcagg attcaatgac attattggac aggagcatct
     1141 gaaagagcat ctgcagaagg caatcaaaac aggaaatctt tcccatgcat atatgatcag
     1201 cggagaaaaa ggctccggaa aagaatttgt tgcaaatatt tttgcaatga cactgcaatg
     1261 tgaaaaaggc ggggtagaac catgtatgga atgccattcc tgcaggcagg ccatgacaaa
     1321 aaaccagccg gatatcatta cggtgacaca tgaaaagccg aatacgatca gtgtggaaga
     1381 tatcagggaa cagatcgtcg gagacgttgc ggtcaagccg tatgcaagta aatggaagct
     1441 gtatataatc aaggacggcg aaaagatgtc cgtacaggca cagaatgccc tgctgaaaac
     1501 gttggaggaa ccgccttcct atgtcattat tttgatcctt acggcgaata gttccatgct
     1561 gcttgaaacg atccgttcca gatgtacgga at
//
LOCUS       DAOSYE010000592         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68516_length_1591_cov_3.905599, whole genome shotgun sequence.
ACCESSION   DAOSYE010000592 DAOSYE010000000
VERSION     DAOSYE010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68516_length_1591_cov_3.905599"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLU43_09595"
     CDS             complement(<1..391)
                     /locus_tag="PLU43_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOO28707.1"
                     /translation="MIFIKERIGKLLCDIKELIYVSDIPIKTYRMIQSEERFENIADL
                     NTDAWTEITNEQLWGGHRAYFWFETMIAIPEALDGKCVVYELKTGREGEWDATNPQFT
                     IYINGIRRQGMDVNHREVLLAESAKAGE"
     gene            complement(414..1235)
                     /locus_tag="PLU43_09600"
     CDS             complement(414..1235)
                     /locus_tag="PLU43_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28708.1"
                     /translation="MKWKFKKTVPYIILTILAIVFALPLLWIAVASLDVNASQAVKLP
                     TEWTLQNYKAVLSDQANIRAFANGLLISFSQSILVVVVAGLAAYPLSRYELRYKNLFM
                     YTILFMTSLPITAVMVPVYKMFLTIGLYDKIPGLVLFLTATSMPYGIWLMKNFMDNVP
                     IELEEAAWVDGASTLTSIIKIIAPLMFPGICVVFIFTFSGSWGNFFVPYILLQTIDKL
                     PASVTLYQFFGQHGMVVYGQLAAYSAIYAIPSILLYVISQNYMSKGFNMAGAAKG"
     gene            complement(1248..>1591)
                     /locus_tag="PLU43_09605"
     CDS             complement(1248..>1591)
                     /locus_tag="PLU43_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28709.1"
                     /translation="QSALDGVPADIEEAAKVDGAGRFQRMIRIVLPCIKNTIATNTML
                     NTLSTLGVFGLIYMMTGGGPGTATLTLPIFMYRQAFISQQLGYGTAISMLILVIGIIL
                     SVFYARIMKND"
BASE COUNT          442 a          395 c          289 g          465 t
ORIGIN      
        1 actcccctgc ctttgcgctt tctgcaagaa gaacttctct gtgattgaca tccattccct
       61 gccgtcttat cccgttgatg tagatcgtga actgcgggtt cgttgcatcc cattcccctt
      121 ctcttccggt ctttaattca tatacgacac attttccatc cagcgcttcc ggtatcgcaa
      181 tcatggtttc aaaccagaag tatgctctgt gtccgcccca caactgctca ttcgtgattt
      241 cagtccacgc atccgtattt agatccgcaa tattttcaaa tctctcctct gactgaatca
      301 tccggtatgt tttgatcggt atgtctgaaa cgtatatcag ctctttgata tcacataaca
      361 gctttccaat acgctctttt ataaatatca tttaaatatc ctccattctt ttgtcagccc
      421 tttgctgctc ctgccatatt gaaccctttg gacatataat tttgtgaaat cacatataac
      481 aggatggacg gaatcgcata aattgccgaa taagccgcaa gctgcccgta tacgaccatg
      541 ccatgctgtc cgaaaaactg atataaggtt accgacgccg gtaatttatc gatcgtctgc
      601 aataaaatat acgggacaaa gaaatttccc cagcttcctg aaaaggtaaa aataaacaca
      661 acacagattc cgggaaacat aagcggtgca atgattttta taatgcttgt cagtgttgat
      721 gcgccgtcta cccaggcggc ttcctccagc tcgatcggaa cattgtccat aaaatttttc
      781 atcagccata ttccatacgg catggacgtt gctgtcagaa acaggacaag ccccggtatt
      841 ttgtcataca gtcctatcgt cagaaacatc ttatataccg gaaccattac tgccgtgatc
      901 ggcaatgaag tcataaataa gatcgtatac ataaataaat tcttatatct gagttcatat
      961 cttgacaatg gatacgctgc cagtcctgca actacaacta ctaatatgga ctgactgaac
     1021 gaaatcagga gtccgttggc aaacgccctg atattggcct gatccgacag caccgcctta
     1081 taattctgca gtgtccactc cgttggaagc tttacggcct gcgacgcatt gacgtctaat
     1141 gaggctactg cgatccacag aagcggaagc gcaaacacaa ttgccagtat tgttaaaata
     1201 atatacggta ctgttttttt aaatttccat ttcattataa caccccctta atcattcttc
     1261 attattcttg cataaaatac acttaagata attcctatca caagaataag catggaaatg
     1321 gctgtaccgt atccaagctg ctgggaaatg aatgcctgtc tgtacataaa aatgggcagc
     1381 gtcagcgttg ctgttccagg tcctccgccc gtcatcatat agatcaaacc gaacacacca
     1441 agtgttgaca atgtgttcag cattgtattc gttgcaattg tatttttgat gcagggaagt
     1501 acaattctta tcattctctg aaaacggcct gctccatcca cttttgcagc ttcttcaata
     1561 tcggccggaa caccgtccag ggcagactga a
//
LOCUS       DAOSYE010000593         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68903_length_1586_cov_2.290660, whole genome shotgun sequence.
ACCESSION   DAOSYE010000593 DAOSYE010000000
VERSION     DAOSYE010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68903_length_1586_cov_2.290660"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLU43_09610"
     CDS             <1..142
                     /locus_tag="PLU43_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO28710.1"
                     /translation="ADAVRIQYGGSVNAGNAAELFAQPDIDGGLVGGASLKADFGKIV
                     NY"
     gene            290..1114
                     /locus_tag="PLU43_09615"
     CDS             290..1114
                     /locus_tag="PLU43_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HOO28711.1"
                     /translation="MSKLQIVIVAIASCVILGAGAMLIKTAMEYKQAESEYENLTQYT
                     AQTDTAQELPAEPVSPVELPEEEEEPVKELRQNLNREDFPDIEVDFDGLKEVNSDFVA
                     WLYVGAVGISYPVVQGEDNEYYLHQTFEDKKNSSGCIFMDWEVKKDLSSWNTFIYGHN
                     MKNGTMFGSLKQFIWNNHLYEKDPYIYLFTEDGIYRYQIYSFYLDSPDSKMYWTCDTI
                     KEYRQYIRTALGKSVYDCQTEATEEDNSVTLVTCSGTGSSKQRFFVHGIFIDRYLY"
     gene            1186..>1586
                     /locus_tag="PLU43_09620"
     CDS             1186..>1586
                     /locus_tag="PLU43_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOO28712.1"
                     /translation="MKHLLFYLKDYKKECIFAPLFKMLEASFELLVPLVIAAIIDKGI
                     GNGNSGYITRGCLFMIMLGFIGLLCSVTAQFFAAKAAVGFSTGLRHDLFRHLLGLSYS
                     EIDTLGTDTMITRMTSDVNQAQTGVNMFLRL"
BASE COUNT          437 a          279 c          401 g          469 t
ORIGIN      
        1 agccgatgct gttcgtattc agtacggcgg atcagtcaat gccggaaatg ccgcagagct
       61 gtttgcacag ccggatattg acggtggtct tgtaggcggt gcgtccttaa aggctgattt
      121 tggtaagatc gtaaattatt aatattccag attttctgat aagcagggag ttccgtcagg
      181 taaaacggat gggctccttg ttttattact ttattcttaa aaaaagtgga cggatccgga
      241 aaaaagatgg taaaattacg tataaagcca aagctttggg agaatgggta tgtcgaaatt
      301 acaaatcgtg attgtggcga ttgcttcctg tgtgattctg ggtgcaggcg caatgctgat
      361 caagactgca atggaatata agcaggcaga aagcgaatat gaaaatttga cgcagtatac
      421 ggcgcagacg gacacggcac aggagctgcc ggctgagcct gtaagtccgg tggagcttcc
      481 ggaagaagag gaagaaccgg taaaagagct gcgtcaaaat ctgaatcgtg aggattttcc
      541 ggatattgaa gtggattttg acggacttaa agaggttaat tcggattttg tggcctggct
      601 ttatgtcggt gcggtcggga tcagttatcc tgttgtgcag ggtgaagata atgaatatta
      661 tctgcatcag acgttcgaag ataaaaagaa cagttccggc tgtattttta tggactggga
      721 agtaaaaaag gacctgtctt catggaatac atttatttat ggccataata tgaagaatgg
      781 aacgatgttc ggttctttga agcaattcat ctggaataat catttatatg aaaaagatcc
      841 ctatatttat ctttttacgg aagacggtat ttaccgctat caaatatatt ctttttatct
      901 ggacagtccg gacagcaaga tgtattggac ttgtgatacg atcaaggaat accggcagta
      961 tatccgtacg gcgcttggaa aatccgtgta tgactgtcag actgaggcaa cggaagaaga
     1021 taattctgtg acacttgtca cctgttcggg cacaggttcg tccaaacagc gtttctttgt
     1081 tcacggtatt tttattgacc gctatttata ttgagtctgt attttttcat aaacagcctt
     1141 cttttttgat atttccccta aataataacg gaaggacaac ggaatatgaa gcatctttta
     1201 ttttatttaa aggattataa aaaggagtgc atttttgcac cgctttttaa aatgcttgag
     1261 gcatcgtttg aattgctcgt gccgcttgtg atcgcggcaa ttatcgataa aggaattgga
     1321 aacggcaaca gcggctatat taccagaggc tgcctgttca tgatcatgct tggctttatc
     1381 ggcctgctct gttcggtaac ggcacagttt tttgcggcaa aggcagcggt tggattcagt
     1441 accggtctgc ggcacgatct tttccggcat ctgctcggac tgtcttattc tgaaatagat
     1501 acgcttggga ccgatacgat gatcacgagg atgacaagtg atgtgaatca ggcacaaaca
     1561 ggtgtaaata tgtttctgcg cctgtt
//
LOCUS       DAOSYE010000594         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_68942_length_1585_cov_3.353595, whole genome shotgun sequence.
ACCESSION   DAOSYE010000594 DAOSYE010000000
VERSION     DAOSYE010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68942_length_1585_cov_3.353595"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLU43_09625"
     CDS             complement(<1..668)
                     /locus_tag="PLU43_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28713.1"
                     /translation="MEWITRLNATIRYMEEHLTEEIDYEQLGRIACCSSYHYQRMFSY
                     MAGIPLSEYIRRRKMSRAAVDLQNSDSKIIDLAAKYNYASPTAFNRAFQSVHGFAPSL
                     ARTSGKTIKSFPPISFQITIKGAVQMDYRIETKEAFRIVGISLPLDKDIEKNFETVPA
                     FWGKNAQNGTVAKLCTKMNAVPSGVLGVSTCEKGTDSWRYFISVASTLPADGFEEYTV
                     PAYT"
     gene            861..1442
                     /locus_tag="PLU43_09630"
     CDS             861..1442
                     /locus_tag="PLU43_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO28714.1"
                     /translation="MGVRSEQKKKRREDILRAGLDLFIRKGYTAATVKDIAEAVGMSV
                     GLLFHYFDSKEALYEELIKYGISGPMGTIAQADMEPIAFFELAAGQIINYIQKDLFAA
                     KMFVLMRQASYSEASPQGVKELFKELDIMTPTIRLIRQGQADGTIRDGDPEALGSAYW
                     CAIQGIAEEYALNPGRPLPDSMWIVDMIRRKKT"
     gene            1439..>1585
                     /locus_tag="PLU43_09635"
     CDS             1439..>1585
                     /locus_tag="PLU43_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO28715.1"
                     /translation="MKKVIIAGAGIAGLTAGIYARQSGFEVTIYESHAIAGGASTSWR
                     RNGYL"
BASE COUNT          448 a          306 c          397 g          434 t
ORIGIN      
        1 caggtatatg cgggtaccgt atattcttcg aatccatctg caggtaaagt gcttgcgaca
       61 gatataaaat agcgccagct gtcggttccc ttttcacagg tactgacgcc aagaacgccg
      121 gaaggtacgg cattcatttt tgtacatagt tttgcgactg ttccgttttg cgcgtttttt
      181 ccccaaaagg caggtacggt ttcaaaattt ttttcaatgt ctttatccag cggtaaagaa
      241 atacctacaa ttcggaacgc ctcttttgtt tcaattctgt aatccatttg cacagctcct
      301 ttgatcgtta tttggaaact gatcggcgga aaggatttta ttgtttttcc ggaagtcctt
      361 gccagtgacg gcgcaaagcc gtggacactc tgaaaagcac ggttaaaagc agttggagaa
      421 gcataattat attttgctgc cagatcaatg atcttactat cactgttctg caggtcaacg
      481 gcggcccggc tcatttttct gcggcggata tattccgaaa gcggaatacc ggccatatag
      541 ctgaacatac gctgataatg ataggatgag cagcaggcga tcctgccaag ctgttcataa
      601 tcgatctcct ccgtaagatg ctcttccata tatctgatcg tggcattcag tcttgtaatc
      661 cattccattt tttcagctcc ttatttgaat tatagaatat gatatcatct ctgtcctctc
      721 ggtttctgca caaaaaagag agtcctttgt attatatcat agaatccaaa tagtatcggc
      781 atagaaaaag tatttcttga caaaaggaca aggtgcatat aatataatga atatatattc
      841 aatgaatggt ggttcaataa atgggtgtgc gcagtgagca aaaaaagaag cgaagggaag
      901 atattttaag ggcaggtctt gatctgttta tcaggaaagg atatacggca gcaacagtca
      961 aagatattgc agaagcagtt ggcatgagcg tgggacttct gttccattat tttgattcaa
     1021 aggaagcgct gtatgaggaa cttatcaaat acggaatttc cggaccgatg ggaacgattg
     1081 ctcaggcaga catggaaccg atcgcttttt ttgaactggc agcaggacag atcatcaatt
     1141 atatacaaaa ggatctgttt gccgcgaaaa tgtttgtttt gatgaggcag gcttcttaca
     1201 gtgaagcatc gccgcagggg gtgaaagaac tttttaaaga acttgatatt atgacgccga
     1261 ccatacggct gatcagacag ggtcaggcag acggaacgat ccgggacgga gatccggaag
     1321 ccctgggcag cgcatactgg tgtgcgattc agggaattgc agaagaatat gcgttaaatc
     1381 caggtcggcc gctgccggac agtatgtgga tcgtagatat gatcaggagg aaaaaaacat
     1441 gaaaaaggta atcattgcag gtgcagggat cgcgggtctg acagccggta tttacgcgcg
     1501 gcaaagcggc tttgaggtga ctatttatga gagtcatgcg attgcaggcg gggcttcaac
     1561 gagctggagg agaaacgggt atttg
//
LOCUS       DAOSYE010000595         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69106_length_1583_cov_2.736911, whole genome shotgun sequence.
ACCESSION   DAOSYE010000595 DAOSYE010000000
VERSION     DAOSYE010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69106_length_1583_cov_2.736911"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..969)
                     /locus_tag="PLU43_09640"
     CDS             complement(28..969)
                     /locus_tag="PLU43_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOO28716.1"
                     /translation="MNKNAKVYVAGHRGLVGSAIVRNLEEKGYSNIIGRTHRELDLTD
                     QAAVRAFFKEEAPEYVILAAAKVGGIHANSTAPADFAYENMQIQCNVIKCAHDFKVKK
                     LLFLGSTCIYPRMAPQPIPEEALLTGPLETTNEAYAIAKISGLEMCKFFKQQYGDDFI
                     SCMPTNLYGPYDNYDLSNSHVMPAMIRKFHEAKVNQAAAVELWGTGNPLREFLYVDDM
                     ADACTFLLENYSGEQHVNIGTGQEVTIRELAETVRQTVGFHGEIIWNKDMPDGTPRKL
                     ADVTKLHALGWTHKVGLKEGVRLAYEWFSKNIDQAKL"
     gene            complement(962..>1583)
                     /locus_tag="PLU43_09645"
     CDS             complement(962..>1583)
                     /locus_tag="PLU43_09645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO28717.1"
                     /translation="AAKLYAFWVTKNYRGAYGLHASNGILFNHESPVRGEDFVTRKIT
                     RGVAAIYKGHQEVLSLGNLDAKRDWGHARDYVEGMWRILQQDTPGDYVLATNETHTVR
                     EFVELAFGEVGIDIEWKGTGVGEKGYDKATGRLLVDVNPRYFRPAEVEFLWGDCTKAE
                     KELGWKRKVNFKKLVSMMVDHDMKAIAGVSTEEFRKQKQIGSKSDE"
BASE COUNT          350 a          483 c          297 g          453 t
ORIGIN      
        1 gaaaggagtt ctttccattt atgttgttta cagttttgcc tgatcgatat tttttgaaaa
       61 ccattcataa gcgagcctga cgccttcttt cagccccacc ttatgcgtcc agccaagcgc
      121 atgcagcttt gtcacatccg caagctttct cggcgtaccg tccggcatgt ctttattcca
      181 gatgatctca ccgtggaatc cgaccgtctg tctgaccgtt tcagccagtt ccctgatcgt
      241 gacctcctga ccggttccga tattcacatg ctgctctccg ctgtaatttt ccagcaaaaa
      301 cgtacaggca tccgccatgt catccacata cagaaattcc ctgagcggat ttccggttcc
      361 ccaaagctct acggcagcag cctgattcac ctttgcctca tgaaatttac ggatcatcgc
      421 cggcatgaca tgagaattgc tcagatcata attatcatac ggtccgtata gatttgtcgg
      481 catgcagctg ataaaatcat ctccatactg ctgtttgaaa aacttgcaca tttccagtcc
      541 tgagatcttt gcgatcgcat acgcttcgtt cgttgtttcc agcggtccgg tcagcagcgc
      601 ttcttccggt atcggctgcg gtgccattct cggataaatg caggtacttc ccaaaaacaa
      661 cagctttttc actttaaaat catgcgcgca tttgatcaca ttgcactgta tctgcatgtt
      721 ttcataagca aagtcggcag gcgccgtact gtttgcatgg atgcctccca ctttggccgc
      781 cgcaaggatc acatattccg gtgcctcctc tttaaaaaaa gctctgaccg ccgcctgatc
      841 cgtcaggtcc agttcccgat gtgtccttcc gataatattg gaatatcctt tttcttctaa
      901 atttcttacg attgcagaac cgacaagtcc ccggtgccct gcaacataga cctttgcatt
      961 tttattcatc cgatttactt ccaatctgct tctgctttct gaattcttcc gtactcacac
     1021 cggcgatcgc tttcatatcg tgatctacca tcatggatac cagcttttta aaattgacct
     1081 ttctcttcca gcccagttct ttttccgcct ttgtgcagtc tccccacaaa aattcgactt
     1141 ccgccggtct gaaataccgc ggattcacat ccacaagcag tctgccggtt gctttatcat
     1201 agcccttttc gccgactccg gtgcctttcc attcaatatc gatcccaact tctccgaaag
     1261 caagctccac aaattcgcgc accgtatgtg tctcgtttgt cgcaagcaca taatcaccgg
     1321 gggtatcctg ctgcaggatc ctccacatac cttctacata atccctggca tgcccccagt
     1381 cccgcttggc atccagattc ccgagactga gaacctcctg atgcccctta taaatcgccg
     1441 ccaccccccg cgtaatcttg cgcgtcacaa aatcctcccc gcgcaccggg ctttcatggt
     1501 taaacaaaat cccgttcgaa gcatgcaacc cgtaagcccc gcgataattc tttgtcaccc
     1561 agaacgcata gagcttggcc gcc
//
LOCUS       DAOSYE010000596         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69111_length_1583_cov_2.584424, whole genome shotgun sequence.
ACCESSION   DAOSYE010000596 DAOSYE010000000
VERSION     DAOSYE010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69111_length_1583_cov_2.584424"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..>1583)
                     /locus_tag="PLU43_09650"
     CDS             complement(121..>1583)
                     /locus_tag="PLU43_09650"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOO28718.1"
                     /translation="TNDGKMTIRSKEQLDQIMKELSGVSFQVTDVKKGERIKKVPLPF
                     TTSTLQQEASKTLNFSTQKTMRLAQQLYEGIDIKGNGTVGVITYLRTDSTRVAEEAQT
                     QATAYITETYGKEYTGETANQAKNQKKIQDAHEAIRPTDISRTPVSLKASLSRDQYRL
                     YTLIWKRFTASRMKEAKYETVSVRIDGNGYRFSVSGSKLVFDGFMAVYSAEEDEKEQS
                     AAFLHTITEKTKLTLDKFDEKQHFTQPPAHFTEASLVKTLEELGVGRPSTYAPTITTI
                     LARRYVTKENRNLYVTELGEVVNKMMKDAFPSIVDMNFTANLELLLDNVEEGTVEWKT
                     IISNFYPDLDEAVKKAEEELAKVTIKEEESDEICENCGRRMVIKYGPHGKFLACPGFP
                     ECRNTKPYLEKIGIKCPKCGKEIVVRKTKKGRRYYGCESGSECDFMTWQRPVETPCEK
                     CGSLMVEKGNKLLCTNEKCGHVMEKNQVTSRNRSAI"
BASE COUNT          363 a          424 c          282 g          514 t
ORIGIN      
        1 tttttcaaat catctgtgta aatagtagca taaataaaca cagaaatcaa tgactcctgc
       61 atttattttg ggaatatttt tactattttt tgatattttc aaactattcc gaactaatgc
      121 tcaaattgcc gacctatttc ttgacgtcac ctgatttttc tccatgacat gcccacactt
      181 ttcatttgtg caaagcagct tatttccttt ttccaccatt aaactgccgc atttttcaca
      241 cggcgtttcc accggacgct gccatgtcat gaaatcacac tcgcttccgc tttcgcagcc
      301 gtaataacgc ctgccttttt tcgtcttccg gacaacgatt tccttcccgc acttcggaca
      361 cttgattccg attttctcca gatacggttt ggtgttcctg cattccggaa agccgggaca
      421 ggctaaaaat tttccatgag gaccatactt gatgaccata cgtctgccgc aattctcgca
      481 gatctcgtct gactcttctt ctttgatcgt tactttcgca agctcttcct cagctttctt
      541 taccgcttca tccagatccg gataaaaatt gctgatgatc gtcttccatt ctacggttcc
      601 ctcttcaaca ttatcaagaa gcagttcaag atttgccgta aaattcatat ctacaatact
      661 cggaaaagca tccttcatca tcttgttcac aacttcccca agctctgtta catacagatt
      721 tctgttttct ttcgtcacat aacgccttgc aaggatcgta gtgatcgtcg gcgcatacgt
      781 actcggcctg ccgaccccca gctcttccag tgtctttaca agcgatgctt ccgtaaaatg
      841 cgcgggcggc tgagtaaaat gctgtttctc atcaaattta tcaagtgtca gctttgtttt
      901 ttccgtaatt gtatgcaaaa acgccgcgga ctgttccttt tcatcttctt ccgccgaata
      961 aaccgccata aatccgtcaa ataccagttt ggaaccggac actgaaaatc tgtaaccatt
     1021 tccatcgatc cggaccgaaa cagtttcata ttttgcctct ttcatgcggc ttgccgtaaa
     1081 gcgtttccag atcaatgtat acagacggta ctgatccctg gaaagagacg ctttcaaaga
     1141 aaccggcgtc cttgaaatat ctgtcggacg gatggcttca tgcgcatcct ggatcttttt
     1201 ttgatttttc gcctgatttg ccgtctctcc ggtatattcc tttccgtatg tttccgttat
     1261 ataagcagtt gcctgtgtct gcgcttcctc cgcaacacgc gtggagtccg tacgcagata
     1321 agtgattaca ccgaccgtcc catttccctt aatatcaatt ccctcataaa gctgctgtgc
     1381 cagacgcatc gtcttctgtg tggaaaaatt cagcgtcttg cttgcttcct gctgcagtgt
     1441 gcttgttgta aaaggcagcg gcactttctt tatgcgctct ccttttttca catccgtgac
     1501 ctgaaaagac acaccggaaa gttccttcat gatctgatca agctgttcct tggacctgat
     1561 cgtcatcttt ccgtcgttcg tac
//
LOCUS       DAOSYE010000597         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69114_length_1583_cov_2.538613, whole genome shotgun sequence.
ACCESSION   DAOSYE010000597 DAOSYE010000000
VERSION     DAOSYE010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69114_length_1583_cov_2.538613"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLU43_09655"
     CDS             <1..452
                     /locus_tag="PLU43_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO28719.1"
                     /translation="RVSFIVGNKKIIHAMKLLRSQYDFGMFLPIQYAAIAALRGPKEG
                     MLQQCRHYQERRDALCGGLRSIGWNVPDSKGTMFAWAKIPEGFSSSQEFCMELVEKTG
                     VICTPGNAFGSMGEGYVRFALVLPVEEIREMIRVIDRSGILKKSGTK"
     gene            465..1451
                     /locus_tag="PLU43_09660"
     CDS             465..1451
                     /locus_tag="PLU43_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HOO28720.1"
                     /translation="MRVLVYNYREFDEKIWFDKYSEELGIELKICREAPQLGQNIDLA
                     HGCDCLSIITSKIPADMVQAFYDQGIRYISTRTIGYDHIDLHKAGELGMKIGNAVYGP
                     QGVADYTIMLMLMCIRKMKRIMQRAEIQDFSLQGIQGRELQDLTIGVIGTGRIGREVI
                     RYLSGFGCKLQAYDLYENEEVKKYAEYVELDKLLRTSDMITLHMPLTDDNFHLINRDS
                     ILAMKDQVVIINTARGGLIDSAALIEGIESGKIGAAGLDVVEKEFGLYYNNLKSEILH
                     NQELAVLRSFPNVIVTPHMAFYTDNAIASMVKNSLTSCKCFINGEDNPWEVI"
BASE COUNT          450 a          293 c          454 g          386 t
ORIGIN      
        1 cgcgcgtgtc gtttattgta ggcaacaaaa agatcatcca tgcgatgaag ctgctgcgct
       61 cccagtatga tttcggcatg tttctgccga tccagtatgc tgcgattgcg gcgctgcgcg
      121 ggccaaagga aggaatgctg cagcagtgcc ggcactatca ggagagaaga gatgcgctgt
      181 gcggcggtct gcgctcgatc ggctggaatg tacctgacag caaaggaacg atgtttgcct
      241 gggccaaaat accggaaggg ttttcctcct cacaggagtt ctgtatggaa ctggtagaaa
      301 agaccggtgt gatctgtacg ccgggaaatg ctttcggcag catgggagaa ggatatgtac
      361 gttttgcgct tgttctgcct gtggaggaga tccgggaaat gatccgggtg atcgacagga
      421 gcgggatctt aaagaaaagc gggacaaagt aaaggagccg gcatatgaga gtattagtat
      481 ataattacag ggagtttgat gaaaaaatat ggtttgataa atacagcgaa gaactgggga
      541 tcgaattgaa gatctgcagg gaagcgccgc agcttgggca aaatatcgat ctggctcatg
      601 gctgtgactg cttaagtatt attacatcca aaataccggc cgatatggta caggcatttt
      661 atgatcaggg gatccgctat atttccacga ggacaattgg gtatgatcat attgaccttc
      721 ataaagcagg agagcttgga atgaaaatag ggaatgccgt ttacggacca cagggcgtgg
      781 cggattatac gatcatgctg atgctgatgt gtatccgaaa gatgaaacgg atcatgcaga
      841 gagctgaaat acaggatttt tccttacagg ggatccaggg aagagaactg caggatctca
      901 cgatcggtgt cattgggaca ggacgcatcg gcagggaggt catccggtat ctgtccggtt
      961 ttggctgtaa attgcaggca tatgatctgt atgagaacga agaagtaaaa aaatatgcgg
     1021 aatatgtgga acttgacaag ctgcttcgga cgagcgatat gatcacgctt catatgccgc
     1081 tgacagacga taattttcat ctgatcaaca gggacagtat tttggccatg aaagatcagg
     1141 tcgtcattat caatacggca cggggcggtc tgatcgattc cgctgcattg atcgaaggaa
     1201 ttgaaagcgg gaagatcgga gcggcaggtc ttgatgtagt tgaaaaggag tttgggctat
     1261 attataataa tttaaaatcg gaaatacttc acaatcagga actggcggtt ctgcgttctt
     1321 ttccgaatgt tatcgtaacg ccgcacatgg ctttttatac ggataatgcg atcgcctcca
     1381 tggtgaagaa ttctctgacg agctgtaaat gctttataaa cggagaagat aatccgtggg
     1441 aagtcatctg agcagagaag cgaatcatat gccggcaggc agcaatgaga aagggtccaa
     1501 tatgaaaaca aagcggatat tgtcacataa atactacctg tatgcaacgg aattttttgc
     1561 cggaatgtcg gtgatggctg tgg
//
LOCUS       DAOSYE010000598         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69199_length_1582_cov_2.463654, whole genome shotgun sequence.
ACCESSION   DAOSYE010000598 DAOSYE010000000
VERSION     DAOSYE010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69199_length_1582_cov_2.463654"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1356)
                     /locus_tag="PLU43_09665"
     CDS             complement(4..1356)
                     /locus_tag="PLU43_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO28721.1"
                     /translation="MKKLAKKLFAAVLAGTMVASLVGCGSSTSDSETSSASDAADTAE
                     TDTAEEAADTGVTLKFQQWWAVECPEGYVQNICDQFKKDTGITVELISAPFADTKTAI
                     ESGAATGTVADLVSVDSSWVYDFADQGVLTDMSTLIDADGFDKSICSDTWDVNGSTYA
                     IPCVNYAYPLFYNKDILDASGVTELPKTWTEFEDVCQKIKDAGYYPFALNLDASSPSG
                     IQNVYMGFAWASGIKFKNDDGTFNVSSNSDLKDFAEFYKDLSDKGYIYPGMASLTESD
                     MTSKFASGEIAFIVNSAAAITAWNKDYPDLNIGAAAIPVKDDYTGQSGNCVASWAVGI
                     TENSDHKAEALKFLEYILSGKDGNDGSIDADLAVTQSGFPGSTLAQPDYSATTEVFQN
                     IYQNYSEGYPINEFTGMKEANTIMVDWINELIPYMDGDQDVDTYLGNVQSAIDEVYGQ
                     "
BASE COUNT          402 a          383 c          289 g          508 t
ORIGIN      
        1 gtcttactgg ccatatactt catcaatcgc agactgcaca tttccgagat aggtatcgac
       61 atcctggtct ccgtccatat atggaatcaa ttcattgatc cagtcgacca tgatcgtatt
      121 tgcctctttc ataccggtaa attcattgat cggatatcct tccgaatagt tctgataaat
      181 attttggaat acttcggtag ttgcactgta atccggctga gccagcgtgc tgcccggaaa
      241 tccggactgt gtcactgcaa gatccgcatc aatagaaccg tcgtttccgt ccttgcctga
      301 aagaatatat tccaggaatt ttaaggcttc tgctttatgg tcactatttt ctgtgattcc
      361 tactgcccag cttgcaacac agtttccgct ctgtcccgta taatcatctt ttaccggaat
      421 cgctgccgca ccgatattaa gatccggata atccttattc caggccgtga tcgctgctgc
      481 agaatttaca ataaatgcga tttctccgct tgcgaactta gatgtcatat cggactccgt
      541 caaagaggcc atgcccggat aaatatatcc cttgtccgaa agatctttat aaaattcggc
      601 aaaatctttc aagtcggaat tgctgcttac attaaacgtt ccgtcatcat ttttaaattt
      661 gattcccgat gcccatgcaa agcccatata tacgttctga attcctgaag gagatgatgc
      721 gtcaagattt aaagcgaacg gataatatcc ggcatctttt attttctgac atacatcttc
      781 gaattcggtc catgtcttcg gaagctccgt aactccggat gcatccagaa tgtccttatt
      841 ataaaataac ggatatgcat aatttacaca gggaattgca taggtgcttc cattcacgtc
      901 ccatgtatcg gaacaaatgc tcttgtcaaa tccgtcggcg tcgatcaatg tactcatatc
      961 tgtcagtact ccctgatctg caaaatcata aacccagctg ctgtctacgc tgacaaggtc
     1021 tgcgactgtt cccgttgcag ctccggattc gattgccgtt tttgtatcgg caaaaggagc
     1081 actgatcaat tcaaccgtaa taccggtatc ctttttaaac tgatcacaga tattctgcac
     1141 ataaccttcc ggacattcaa ctgcccacca ctgctgaaat tttagtgtta cgccggtatc
     1201 cgcagcttct tctgcagtat cagtttctgc agtatcggct gcatccgatg ccgatgaagt
     1261 ttcggaatca ctcgtactgc tcccacatcc tacaaggctt gcaaccatgg ttcccgccaa
     1321 tacagctgca aatagctttt ttgctaactt tttcattact ttttcctcct ttttatttgt
     1381 gttaccaatt tggatagttt cagtatagca ctgtgaaatt atatttcaac atttatgtta
     1441 ttgtgaaatg ttctttctct gttttgcaca atttgcaggt gcaaatttta ttcacttttt
     1501 cttaattatg aaattatatt tcatgataaa tcgagtagga gggagtgatt agctcccgtc
     1561 ctctcacagc accgtgcgta cc
//
LOCUS       DAOSYE010000599         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69256_length_1581_cov_2.762779, whole genome shotgun sequence.
ACCESSION   DAOSYE010000599 DAOSYE010000000
VERSION     DAOSYE010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69256_length_1581_cov_2.762779"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1200
                     /locus_tag="PLU43_09670"
     CDS             121..1200
                     /locus_tag="PLU43_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HOO28722.1"
                     /translation="MKKRLRFFKENGKGLLLCLLIALPVWILTKEIKSLELVGAPILA
                     ILAGMLLPGTIKENSVYQSGITFTSKKILQGAVVLLGFGLNLATIGQVGAASLPIILS
                     TITTSLVIAWLINKIVRLPYKTAALIGIGSSVCGGSAIAASAPVINADEEEIAQAVSV
                     IFLFNVIAAFIFPTLGGMIGLSDTGFGLFAGTAVNDTSSVTAAASTWDTLHQTGGKVL
                     EYATIVKLTRTLAIIPITLGLSLLELKKAKNTDKKSGEQGSGFSLRSVFPMFILYFVL
                     ASVITTAAVTILSGNALIMADTLFSFLKQLSKFMIVMAMAAIGLNTNLAKLIKKGPRP
                     FLLGFACWISITAVSLLLQKALGLI"
     gene            1346..>1581
                     /locus_tag="PLU43_09675"
     CDS             1346..>1581
                     /locus_tag="PLU43_09675"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO28723.1"
                     /translation="MSRFTLPRDIYHGKGCLSELKNLKGKKAILVVGGSSMKKQGFLD
                     KAVGYLKEGGMEVKLFEGVEPDPSVETVMKGAQA"
BASE COUNT          461 a          305 c          365 g          450 t
ORIGIN      
        1 tacgcggtgc ttcacaccgc ttaatgaatt atcatgaata attcaggatt aatttacatt
       61 atatataata tgctatttaa tagcagtaat gcaataaata ttttttgaag gagcgataaa
      121 atgaaaaaaa ggttgcgttt ttttaaggaa aatggaaaag gactgctgct gtgcctgctt
      181 attgccttgc cggtctggat cttaacaaaa gaaataaaat cactggaatt ggtcggagct
      241 ccgattcttg ccattctggc cggaatgctg ctgccgggaa cgatcaagga gaactccgtt
      301 tatcaaagcg gaattacgtt tacatctaaa aagattttac aaggggccgt tgttttgctt
      361 ggctttgggc tgaatctggc aaccattgga caggttgggg cagcgtccct gccgatcatt
      421 ctctctacga taacaacatc ccttgtaata gcatggctga tcaataaaat agtccgtctt
      481 ccgtacaaaa cggcagctct gatcggaatc ggctcttccg tatgcggcgg gtctgcgatc
      541 gctgcttccg cgcccgtgat caatgcagac gaagaagaga tcgcacaggc tgtttcagtc
      601 atttttttat ttaatgtgat cgctgccttt atatttccga cacttggggg catgatcgga
      661 ctttccgata ccggtttcgg tttatttgca gggacagccg taaatgatac gtcgtctgtt
      721 accgctgccg cctcgacatg ggacaccttg catcagacag gcgggaaagt actggaatat
      781 gctacgatcg taaaactgac aagaacactg gccattattc cgatcacgct tggtctgtct
      841 ctgctggaac taaaaaaagc gaaaaacacc gataaaaaat cgggagagca gggaagcggt
      901 ttttcattga gaagtgtatt tccgatgttt attctttatt ttgtgcttgc atccgttata
      961 acgaccgcag ccgtaacgat tctttccggg aatgcactga ttatggctga tacattgttc
     1021 tcctttttaa aacagcttag caagtttatg atcgtaatgg cgatggcagc gatcggactc
     1081 aataccaatc ttgcaaaatt gataaaaaaa ggtccgcgcc cgttccttct tggcttcgca
     1141 tgctggattt ccataacggc tgtcagcctg ttactgcaga aagcacttgg attgatataa
     1201 gtttgatata aatatgtcat aattgttaat ttttttacaa tgtgaaaaaa aaggcttccc
     1261 attttacaga aaaggtggta aaatccatca tgtagatatt gttaaataaa taacaatatg
     1321 tagaaaagaa ggaggaaaat caattatgag cagatttact ttaccaagag atatttacca
     1381 tggaaaggga tgtctctccg aattaaaaaa tctgaaaggg aaaaaagcca ttctggttgt
     1441 cggcggcagt tccatgaaaa aacagggatt tttggacaag gcagtcggct atttgaagga
     1501 aggcggcatg gaagtgaagc tatttgaagg tgtagagccg gatccttccg ttgagaccgt
     1561 tatgaaaggc gcacaggcca t
//
LOCUS       DAOSYE010000600         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69258_length_1581_cov_2.734600, whole genome shotgun sequence.
ACCESSION   DAOSYE010000600 DAOSYE010000000
VERSION     DAOSYE010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69258_length_1581_cov_2.734600"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..515)
                     /locus_tag="PLU43_09680"
     CDS             complement(108..515)
                     /locus_tag="PLU43_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO28724.1"
                     /translation="MDIVNIDHITINVLSMSESIYFYETVLGLKKDSYILMEDHAIQY
                     FVLSKTTKLELIRYQYETKRQKPACDDRGIYRHFAIEVKDLDKEYQKIAGYANVKITA
                     KPARCEKLFFRNFILEDPNGVEIEIIEREMEKG"
     gene            complement(606..>1581)
                     /locus_tag="PLU43_09685"
     CDS             complement(606..>1581)
                     /locus_tag="PLU43_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28725.1"
                     /translation="SYQNQFYQAAFQGADDAAADFGVTVVTNGPDEESNVSQQVEQVA
                     AAINQKPDAIVLAACDPSALEEVLTKAKEDGIPVIGFDSGVPGDTTGAVLATAATDNE
                     NAGANVADNMVADEDFQKAITAGTEDAPTVIGVLAQDATSGSITLRVDGFINEMVAQL
                     EALDGLDGCVDVSGQEKWTIPSENAAKVKIVVTVPPSTSQADIQSAANSIFAMDNLVG
                     VFAANQDAVNGVISATNDATDLDKETGKYKDIFVAGFDAGKSQKDAISNGQFLGSVTQ
                     DPYQMGYQAIRLAVDAADGKEVSDVDTGSKWYNKDNITDEDIAILLYD"
BASE COUNT          368 a          438 c          286 g          489 t
ORIGIN      
        1 actcaggact ctgttctgta tggagctgcc attactgcga tcgaccacat tttggaaaag
       61 gctctcgatt ttgaaaaata aatgctgctg catccgcatt ttacagctca gcccttttcc
      121 atttctcttt ctatgatctc gatctccaca ccgttcggat cttccaatat aaagtttcta
      181 aaaaataatt tttcacacct ggcaggcttt gccgttattt tcacattggc ataccctgct
      241 attttttgat attctttatc cagatctttc acctcgatcg caaaatgcct gtagattccc
      301 ctgtcgtcgc aggccggttt ctgtcttttc gtttcatatt gataccggat cagttccagc
      361 tttgttgttt tgcttaatac aaaatactgg atcgcatgat cttccatcaa aatatagctg
      421 tcctttttca gtccaagcac agtttcataa aaataaatgc tctcgctcat cgacagcaca
      481 ttgatcgtga tatgatcgat atttacaata tccatacttt cccctccgcc ttgtttttat
      541 acagcagcag gcagccccta tccttttaaa cagaagctgc ctgccgtata ctttaagcaa
      601 ataaattaat catataacag aatcgctata tcttcatccg taatattatc cttgttatac
      661 catttggaac ccgtatcgac atccgatact tcttttccgt ccgcagcgtc aacagccagg
      721 cggatcgcct gatatcccat ctgataagga tcctgcgtca cagagcccaa aaactgtccg
      781 tttgatatcg catccttctg ggatttgccg gcatcaaatc ctgctacgaa aatatctttg
      841 tatttcccgg tttccttgtc cagatccgtc gcatcgttcg ttgcgctgat cacgccattg
      901 actgcatcct gatttgccgc aaaaacacct accagattat ccatggcaaa aatactgttt
      961 gcagccgact gaatgtctgc ctggcttgtc gaaggcggaa ccgttacaac aatttttact
     1021 tttgccgcat tttcggacgg aatcgtccat ttttcctgtc ccgatacatc aacacagccg
     1081 tccagtccat ccagtgcctc cagctgtgca accatttcat taataaatcc gtctacacgc
     1141 agtgtgatag agccgctcgt tgcatcctgt gccagaacac cgatcacagt cggggcatcc
     1201 tccgttcctg cagtgatcgc tttttgaaaa tcttcgtcag caaccatatt atcggcaaca
     1261 tttgcgcccg cattttcatt gtccgtggct gcggttgcca gtacggctcc tgtcgtatct
     1321 cccggaacac cggagtcaaa acctattacc ggaattccgt cttcttttgc ctttgtcagc
     1381 acttcttcca gtgcgcttgg atcacatgcg gcaagcacga tcgcgtccgg cttttggttt
     1441 atcgctgccg ccacctgttc tacctgctgt gaaacattac tttcttcatc cgggccattg
     1501 gtaacgactg tcactccaaa atcggccgca gcatcatcgg ctccctgaaa ggccgcctga
     1561 taaaactgat tctgatagct t
//
LOCUS       DAOSYE010000601         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69414_length_1579_cov_1.995407, whole genome shotgun sequence.
ACCESSION   DAOSYE010000601 DAOSYE010000000
VERSION     DAOSYE010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69414_length_1579_cov_1.995407"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLU43_09690"
     CDS             <1..985
                     /locus_tag="PLU43_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28726.1"
                     /translation="AYCRSIGIETFVAMDIQDLNEILEILWVRKAVANTRALVLTAGS
                     QPTFGIQSLIRDPEILRQKYGFEVVKLPFNSIVPYMEQITDEEAKPLADQIINGSRET
                     KVNTDWFLNDIKYYMAAKKMMDVYDCNAFSTACHELCTSEIPQNMKFTPCVCHSIMKD
                     EGIPSGCEEDLNALLAMTIMQYAAYRPAFMGNPNYETDELLRIHHAVPALCMNGYGTK
                     PLQYKLWAFTGQGFGGKLQVDFTENNEDAVTLARFNPLGDTMCVKTGEVLRSEYDEIY
                     CSPYYYIKMDDARTYMHNLASFGHHQVLIFGDYTKLLKKIAKVMKFQILEG"
     gene            1003..>1579
                     /locus_tag="PLU43_09695"
     CDS             1003..>1579
                     /locus_tag="PLU43_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO28727.1"
                     /translation="MIYLNNAATTYPKPQCVLDAHAAALCTIPAGQFRSAVDKTGKDV
                     FQSCRESLGRLFHIADAQRIYFSFGATDSANKLINGLDFSGKTVITTQTEHNSILRPL
                     MNHPVYKEQLLILPCDSFGKVDIAPMERLKKGEAAALFINHCSNVTGMIQDIGEITRL
                     AREKDIRVFADVSQSAGCIPIDADGWGLDGLI"
BASE COUNT          428 a          345 c          419 g          387 t
ORIGIN      
        1 cgcctattgc cgttcgatcg ggatcgaaac attcgtagcc atggatatac aggatctgaa
       61 cgaaatactg gagatcctct gggtcagaaa agccgttgcc aatacaaggg cactcgtatt
      121 gacggcaggc tctcagccga cattcggcat tcagagtctg atccgtgatc cggagatcct
      181 taggcagaaa tacgggtttg aagtggtcaa gcttccgttt aattcgatcg ttccctatat
      241 ggaacagatc acggatgagg aagcgaagcc cctggcagat cagatcatca acggttccag
      301 ggaaacaaaa gtaaatacgg actggttcct caatgatatc aaatattata tggctgccaa
      361 aaagatgatg gatgtttatg actgcaatgc tttttcaacg gcatgccatg agctgtgtac
      421 ttccgagatc ccgcagaata tgaaattcac gccctgtgta tgccattcca ttatgaagga
      481 cgaaggaatt ccaagcggct gtgaggaaga tctgaatgcc ctgcttgcga tgacgatcat
      541 gcagtatgcc gcatacaggc ctgcttttat ggggaatccg aattatgaga cagacgagct
      601 gcttcggatc catcatgcgg ttccggccct ttgcatgaac ggatacggca caaagccgct
      661 gcaatataaa ctctgggcgt ttacgggcca gggcttcggc ggaaagctgc aggtggattt
      721 cacggaaaat aacgaggatg cggtcacact ggcccgcttt aatcctcttg gcgatacgat
      781 gtgtgtgaag acaggggaag tgcttcgttc cgaatatgat gagatatact gcagtccgta
      841 ttattatatc aaaatggatg atgcaagaac ctatatgcat aacctggcaa gcttcggaca
      901 tcatcaggta ctgatttttg gtgattatac aaagcttttg aaaaagatcg caaaggtaat
      961 gaaatttcag atccttgaag gctgatgcgg aggaatttcg gtatgatcta tttgaataat
     1021 gccgcaacta catatccgaa gccgcagtgt gtgctggatg cacatgctgc ggctttgtgc
     1081 acaataccgg caggacagtt cagaagtgca gtggacaaaa cagggaaaga tgtgtttcaa
     1141 agctgcaggg agagccttgg cagactgttt catatcgcgg atgcgcagcg catctatttc
     1201 tctttcggcg caaccgattc agccaataag ctgatcaacg gccttgattt ttcagggaaa
     1261 acggtcatta cgacgcagac ggagcataac agtattctgc ggccgctgat gaatcatccg
     1321 gtttataaag agcagcttct gatcctgccg tgtgattctt ttggaaaggt cgatatcgcg
     1381 ccgatggagc ggctcaaaaa aggagaagct gctgcactgt ttattaatca ctgttccaat
     1441 gtgaccggca tgatccagga tatcggcgag atcacccggc tggcaagaga aaaggatatc
     1501 agggtctttg cggacgtatc ccaaagcgcc ggatgcattc cgatcgatgc ggacggatgg
     1561 gggctcgacg gccttattt
//
LOCUS       DAOSYE010000602         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69742_length_1574_cov_4.146149, whole genome shotgun sequence.
ACCESSION   DAOSYE010000602 DAOSYE010000000
VERSION     DAOSYE010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69742_length_1574_cov_4.146149"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1574
                     /locus_tag="PLU43_09700"
     CDS             219..>1574
                     /locus_tag="PLU43_09700"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOO28728.1"
                     /translation="MEVTAKLAAEFHTILLPNILMLVLLVSFSGYVYFRAKQTALLYS
                     YLSVTCTIALWMLSKICKTLSPTVSLRWFFIVIQYFAIDSLGVCFLIFSYIYRNKKKP
                     PLRYFCLWSILPLLSFLILVTNPLHMTFYSYFDIHRDRFGLSFYLAQSVHYVYLITGV
                     IQLSKGFTKQPGFSGNQALGRLFSVFVLLPLFANIYYIFFKIGLFHWIFCFPVFDFTP
                     IAASLSLILFIIPTLKYRFFDISPISLGNLYDMVPHGLILMDKKGRLYGGNQAFFQLF
                     CLKSPPASLTRLFQDPALFTKKNTEQLIRFLQDPCAPETELLLADERLIQIQKHARHN
                     GHMLLLFHDITMLNRNTRLLGEQKKELEIINSRLTGMADTTKELAVARTKSQIAQNVH
                     DILGHSLTVVIGTAELAARGKREDAFQKLLQIEELLTGSMNDLKNALNGNGMKLGETT
                     LI"
BASE COUNT          406 a          370 c          305 g          493 t
ORIGIN      
        1 gttcatggag atcacctgcc ttttttaaga tacttgtatt ataaattatt ggggagtcat
       61 ccgtgcagtg tctgcagtca tgaaatacgt cctttcctgt tgtgcggcag tcatgaaaaa
      121 tgactctctt tccacaaaaa agtgacttct taaatttgat acattgtgat ataatatgat
      181 acagtaaaat cctgttctgt agcggaggtt ctttattgat ggaagtaacg gcaaaactgg
      241 cggcggaatt tcatacaatc cttcttccca acatcctgat gctggtcctg ctcgtttctt
      301 tttccggtta tgtgtacttc agggcaaaac agactgctct tttatatagt tacctgtccg
      361 tgacctgcac gatcgcactc tggatgctga gcaaaatatg caaaacactt tctccgaccg
      421 tctcactgcg gtggtttttt attgtcattc aatactttgc cattgattct ctcggcgtct
      481 gttttcttat tttttcttac atttaccgaa ataaaaaaaa gccccctctc cggtatttct
      541 gtctttggag cattcttccg ctgctttctt ttcttatact tgtcacgaat ccgcttcaca
      601 tgacgtttta ctcttatttt gatattcacc gggacaggtt tggactctct ttttatctgg
      661 cgcagagcgt acattatgtc tatctgatta ccggtgtgat ccagctctca aaaggattta
      721 caaagcagcc cggattttcc ggaaatcagg cgcttggccg cttgttttcc gtttttgtac
      781 tgcttccgct ttttgctaat atttattata tcttctttaa aataggactt tttcattgga
      841 tcttttgttt tccggtattc gattttacac cgatcgccgc atccctttca ctgattcttt
      901 ttataatacc gaccttaaaa tatcgtttct ttgatatttc tcctatttcc ctcggaaatt
      961 tatacgacat ggtgccccat ggactgatcc tcatggataa aaaaggacgt ctctacggcg
     1021 gcaatcaggc cttttttcaa ctgttttgcc tgaaaagtcc ccccgcttcc ctaacgcggc
     1081 tttttcagga tccggcgctt tttacaaaaa aaaataccga acagctcata cgatttctgc
     1141 aggacccctg tgctcctgaa acagaactgc tgctggccga tgaacgtctg atacagatac
     1201 agaaacatgc cagacacaac ggacatatgc tgctgctctt tcatgatatt accatgctaa
     1261 accgcaacac acgcctttta ggtgaacaaa aaaaggagct ggaaatcatc aattcccgcc
     1321 tgaccggtat ggccgacaca acaaaagaac tcgccgtcgc ccgtacaaaa tcgcagattg
     1381 cgcagaatgt acacgatatc ctcggacata gcctgaccgt agtgatcggc acggctgagc
     1441 tggctgccag aggaaaaaga gaagacgctt ttcaaaaact gcttcaaatc gaagaattgc
     1501 tgaccggcag tatgaatgat ctgaaaaacg ccctgaatgg aaatggaatg aagcttgggg
     1561 aaacaacatt gata
//
LOCUS       DAOSYE010000603         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69795_length_1574_cov_2.226465, whole genome shotgun sequence.
ACCESSION   DAOSYE010000603 DAOSYE010000000
VERSION     DAOSYE010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69795_length_1574_cov_2.226465"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..>1574)
                     /locus_tag="PLU43_09705"
     CDS             complement(69..>1574)
                     /locus_tag="PLU43_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOO28729.1"
                     /translation="PATGNRRLMQDILRKEMGFAGVLISDWAAIEELVFHGVAADKKE
                     AAQLAMNAGVDIDMCTNSYIKNIEPLIGEGRLTMEQLNEAVLRVLELKNRLGLFENPY
                     RFADEKKAEEVILCREHRALAREAASSSMVLLKNDGQLLPLQEKGKRIALIGPYADCK
                     HLYGAWSLLGRDEDTVSIREGLTELSPDDTITFAKGCYMLEKGEKLYGFRGLVKEERP
                     LSEEQLMDEAVREAEQADLVVLALGECAYHSGEGGSRADIRLPKEQRKLLKKIAGVNK
                     NIAVVLFHGRPLDIRDVTKYARAVLAAWFPGTEGGNAVCDILFGRKNPSGRLSMSFPY
                     STGQVPLSYHHLATGRPYNGDERNRFQSRYLDAPTEPLYPFGYGLSYTDFVYSKVNLT
                     KTSLSKDKADDSITASLEVRNTGPLEGTETVQLYIQDICGSVARPVRELKGFRQITLV
                     PGESRRVEFTVTEEMLRFTTADNCFESEPGKFRIFIGADSTTTNAVEFLLK"
BASE COUNT          372 a          448 c          313 g          441 t
ORIGIN      
        1 ggtctcttcc ttttttattt cttatttttc ttgccaacga taattatact ctaagttgga
       61 ggtaaccgtc attttaacaa aaattcgacg gcatttgttg tagtactgtc tgccccgata
      121 aaaatacgga atttacccgg ctcactttca aagcaattat ccgccgttgt aaaacgaagc
      181 atttcttccg taactgtaaa ttcgacccgc ctgctctctc cgggaacaag cgtgatctgc
      241 cggaaacctt tcaattcacg gaccggccgc gccacactgc cgcagatatc ctgtatataa
      301 agctgcaccg tctctgttcc ttccagcgga cccgtatttc tgacttccag ggaagcagtt
      361 atactgtcat ccgctttatc ttttgaaagc gatgttttcg taaggttcac tttcgaataa
      421 acaaaatccg tataggaaag cccatagcca aacggatata acggttctgt cggcgcatcc
      481 agatatctgg actgaaaccg gttcctttca tctccgttat acggtcttcc cgtagctaaa
      541 tgatgataag acagaggtac ctgaccggta ctgtacggaa acgacatgga aagtctgccg
      601 ctcggatttt tcctgccaaa caaaatatca cagacagcat ttccgccctc cgtacccgga
      661 aaccaggctg ccagaactgc tcttgcatac tttgttacat ccctgatatc aagcggacgt
      721 ccatgaaaca gtacgaccgc aatattttta ttgactccgg caattttttt caacagcttt
      781 ctctgttcct taggcagccg gatatcggca cggcttccgc cttctccgct gtgataggca
      841 cattcgccga gtgcaagcac gacaagatct gcctgttcag cctcccggac cgcctcgtcc
      901 atcagctgtt cttctgaaag gggccgctcc tcttttacaa gtcccctgaa cccataaagc
      961 ttttcaccct tttcaagcat gtaacagcct ttggcaaatg taatcgtatc atccggtgac
     1021 agctccgtaa gaccctccct gatcgaaacg gtatcctcat cccttccaag cagtgaccat
     1081 gcaccgtaaa gatgcttgca gtccgcatac ggtccgatca gcgctattct ttttcccttc
     1141 tcctgaagcg gaagcagctg tccgtcattt tttaagagca ccatggaaga ggatgccgcc
     1201 tctcttgcaa gtgcacggtg ttccctgcat aggatcacct cttctgcctt tttttcatcg
     1261 gcaaaacgat aggggttctc aaacaggcca agcctgtttt ttaattccag tacccgaagg
     1321 accgcctcat tcagctgctc catggtaagt ctgccttccc cgatcagcgg ttcgatattt
     1381 ttgatatagg aatttgtaca catatcaatg tccacacctg cattcatcgc cagctgcgca
     1441 gcttcttttt tatctgcagc aacgccgtgg aaaaccaatt cttctatggc tgcccagtcg
     1501 gagatcaaaa cgcctgcaaa ccccatttcc tttctgagga tatcctgcat cagccgcctg
     1561 tttccggtcg ccgg
//
LOCUS       DAOSYE010000604         1572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69918_length_1572_cov_2.630191, whole genome shotgun sequence.
ACCESSION   DAOSYE010000604 DAOSYE010000000
VERSION     DAOSYE010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69918_length_1572_cov_2.630191"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLU43_09710"
     CDS             complement(<1..280)
                     /locus_tag="PLU43_09710"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO28730.1"
                     /translation="MKIGGREFELDKNTYVMGILNVTPDSFSDGGSYCTIDTALKRTE
                     QMIAEGADVIDVGGESTRPGHSVIGDNEEISRVVPVIERLKSSFDIPVS"
     gene            complement(325..816)
                     /locus_tag="PLU43_09715"
     CDS             complement(325..816)
                     /locus_tag="PLU43_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO28731.1"
                     /translation="MEKMERVNRILSDEEFRIKCREIAVLEKDRIYCKHQIDHFLSVA
                     RIAVLLCEEKDLTIDRELIYAAALLHDIGRGVQYQTGERHELASIKIAPDILKRSGFY
                     EEEIAEIIEAIANHRNKEIEQCSDLNGIFYRADKLSRNCFCCEAESLCDKETAKKTLF
                     LYY"
     gene            complement(847..1398)
                     /gene="folE"
                     /locus_tag="PLU43_09720"
     CDS             complement(847..1398)
                     /gene="folE"
                     /locus_tag="PLU43_09720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOO28732.1"
                     /translation="MDRDKIERGIRLVIEGIGEDPGREGLLETPERISRMYEEIFGGL
                     TENPKAYLEKTFQAENNEMIVEKDIIFYSVCEHHLMPFYGSAHIAYVPDGKVVGLSKL
                     ARTVDVYAKRPQIQEKMTAQIADALMEYLQPKGVMVMLTAEHLCMTMRGIRKPGSKTV
                     TVVTRGVFEKNRELKDEFFQMLS"
BASE COUNT          369 a          450 c          248 g          505 t
ORIGIN      
        1 tcgacaccgg aatatcaaag ctgctcttca gtctttcaat aacaggtact accctgctta
       61 tttcctcatt gtcgccgatc accgaatgac ccggtcttgt agattcaccg cctacgtcga
      121 tcacatccgc cccctcggcg atcatctgct ccgtccgttt cagtgccgta tcaattgtac
      181 agtaacttcc gccgtccgaa aaagaatcag gtgttacatt taatattccc ataacatatg
      241 tatttttgtc cagttcaaac tccctgccgc caattttcat tctgtcaccc tttccatact
      301 atcctgtcag caactatact ccccttaata ataaagaaac aatgtttttt tcgccgtctc
      361 cttatcacat aaagactctg cctcacagca gaaacagttt ctcgacagct tatccgcccg
      421 gtaaaaaata ccgttcagat cagagcactg ctcgatttcc ttatttctgt gattggcgat
      481 cgcttctatg atttctgcga tctcttcttc ataaaaaccg cttctcttta aaatatcagg
      541 tgcaattttt atacttgcca attcatgccg ctctcctgtc tgatactgca cacctctgcc
      601 gatatcgtgc agcagggccg ccgcataaat caattcacga tctattgtaa ggtctttttc
      661 ttcacaaagc aggactgcga ttctggcaac actcaaaaaa tggtcgatct ggtgcttaca
      721 atagatccgg tccttttcca ggactgcaat ttccctgcat tttatccgaa attcttcatc
      781 cgataatatt ctgtttacac gttccatctt ctccattgtt tctcctccat actgccatac
      841 ggcctcttat gaaagcattt gaaaaaattc atcctttaat tccctgttct tttcaaatac
      901 gccgcgcgtt acgaccgtaa ctgttttgct gcccggtttt ctgattccgc gcatcgtcat
      961 acacagatgc tccgccgtta acatcaccat gactcctttt ggctgtaaat actccatcag
     1021 cgcatctgca atctgcgcag tcatcttttc ctggatctgc gggcgttttg catatacgtc
     1081 cactgttctg gccagcttgc ttaaccccac tacctttccg tccggcacat aggcaatatg
     1141 cgcacttccg taaaaaggca tcagatgatg ctcacagaca gaatagaaaa tgatatcctt
     1201 ttcgacaatc atttcattat tttccgcctg aaaagttttt tcaagatatg ctttcggatt
     1261 ttccgtaagt cctccgaata tttcttcgta cattctggaa attctctccg gcgtttccaa
     1321 aagtccctct ctgcccggat cttctccaat tccttctata accagcctga ttccccgttc
     1381 gatcttatct ctgtccatcc ttaccgtcac tcctgcttta tttatatatt aatgattatt
     1441 tttcacttct ttatcaacga tcctcttcag ccctgccaga tatgccaaag atccgaacgc
     1501 cacgatcagc ggcttttcag accggtatcg ggcagccatt tccagacttc ttttcactgc
     1561 cagctgcagg ga
//
LOCUS       DAOSYE010000605         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_69972_length_1571_cov_3.288259, whole genome shotgun sequence.
ACCESSION   DAOSYE010000605 DAOSYE010000000
VERSION     DAOSYE010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69972_length_1571_cov_3.288259"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>1571
                     /locus_tag="PLU43_09725"
     CDS             130..>1571
                     /locus_tag="PLU43_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28733.1"
                     /translation="MTKIKKMLSNPICFLFAVNVIVLLVSVFVFHPFFEENDDAFLAM
                     LAEGAYGMREDHLIYVNAVLGKLLTGVYGLIPGIRWYSILQYLFLFAAYCIMTYLLGV
                     QRKRPLLSFLLLLGTFYETYVSLQYTKTAAVVTAAGYLLLFEIVRIKKEIGRFPRLFF
                     LPAYTLLLYGALLRGSAFLLASIPMLFVGLYEWHCFYRKEERKEFVKVFVPVFCVVAL
                     LFAADHMAYENDAEWKTFLAYNNTRMELLDYRYDLLDYSANGRQLEELGISENDALLY
                     LTWQFGDDRVVTTEFMNNILDHANARSVDTKMLKEFLSHLFEEFYSLNALLPCLLGAV
                     LLFLTAFSLKDGESRKKKGLFLYFAGNVFAVFLILFYYQYSGRWNHRVVYALLMTVLF
                     AFFYEGAFCYDGQKEKVRAEMIIFAIALFFNIGLLLQNQFDYRSYLRTELDSKAQTAL
                     MQKEKEQLFVIDTFTFQNAYKYDVFTAYLPG"
BASE COUNT          370 a          292 c          402 g          507 t
ORIGIN      
        1 acctgtatat atggcagctt ggacagcggc actattgctg atctatatcg ttccgatcgt
       61 atcgggtatc ctgtttttag tgatctgatc gtgacgccta agaggacaaa ggattaacag
      121 gagtaaagga tgacaaaaat aaaaaagatg ctatccaatc ccatatgttt tctatttgcg
      181 gtcaatgtaa ttgtactgct tgtttccgtg ttcgtatttc atcctttttt tgaggaaaat
      241 gacgatgcgt ttcttgcaat gcttgcagag ggcgcctatg gaatgaggga agatcatctg
      301 atctatgtca atgcggtcct ggggaagctt ttaacgggag tatacggtct gattccgggt
      361 atccggtggt attccatact gcagtatctg tttttgtttg cagcgtactg cataatgacc
      421 tatctgcttg gcgtgcagag aaaaaggccg ctgctttcgt ttcttctgct gcttggcacg
      481 ttttatgaaa cgtatgtgtc gctgcagtat acaaagactg cggcagttgt gactgcggcc
      541 gggtatcttc tgctgtttga gatcgtgcgc ataaaaaagg aaatcggcag attcccgcgc
      601 ctgttttttc tgcctgcgta tacgctgctt ttatatgggg cgcttttgcg cggcagcgca
      661 tttttgcttg caagtatacc gatgcttttt gtcgggctgt atgaatggca ttgtttttac
      721 aggaaagaag agcggaaaga gtttgtaaag gtgtttgttc cggtcttctg tgtggtcgcg
      781 cttctgtttg ccgccgatca tatggcatat gaaaacgatg cggaatggaa aacgttcctt
      841 gcctataaca atacccgtat ggagcttttg gattacaggt atgatctgct ggattattcg
      901 gcaaacggca ggcagcttga agagcttggc atttctgaaa atgatgcact tttatatctg
      961 acatggcagt ttggggacga cagggtcgtt acgacagaat ttatgaataa catactggat
     1021 catgcaaatg cgcggtccgt tgatacgaag atgctgaaag aatttttatc acatttgttt
     1081 gaagagtttt attccctgaa cgcgcttctg ccgtgtctgc ttggtgcggt tttgttattt
     1141 cttacggcgt ttagtctgaa agacggggaa tcccgcaaaa agaaaggttt attcctttat
     1201 tttgccggga atgtctttgc ggtttttttg attttgtttt actatcagta cagcggcaga
     1261 tggaatcaca gagttgttta tgcattgctc atgacggtat tgtttgcgtt tttttatgaa
     1321 ggagcgttct gttatgacgg tcagaaggaa aaagtccggg cggaaatgat catatttgcc
     1381 attgccctgt tttttaatat cggactgctg cttcagaatc agttcgatta ccggtcttat
     1441 ctgcggaccg agctcgacag caaagcacag actgcgctta tgcaaaaaga gaaggagcag
     1501 ctgtttgtca ttgatacgtt tacgtttcag aatgcttata aatacgatgt atttacggcg
     1561 tatctgcccg g
//
LOCUS       DAOSYE010000606         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70236_length_1568_cov_3.269663, whole genome shotgun sequence.
ACCESSION   DAOSYE010000606 DAOSYE010000000
VERSION     DAOSYE010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70236_length_1568_cov_3.269663"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLU43_09730"
     CDS             <1..450
                     /locus_tag="PLU43_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28734.1"
                     /translation="LEKYVQAWIGQASTALWALCVMDIWVAMGFYSVIFYGALLDIPG
                     DVIEAARIDGCNGFNLFRYVLSPLIKPVTITALVFSFSGTVKMFESATALTGGGPGNA
                     TKSLSMYMYNVAFSYNKVGYGSVIALVIFLICIAGSFVINLFDRQKD"
     gene            465..1301
                     /locus_tag="PLU43_09735"
     CDS             465..1301
                     /locus_tag="PLU43_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28735.1"
                     /translation="MKAWKKTKAWLVRILIFLLCMVEIYPIFWMLTASLKKQSEWTSK
                     PAYALNEGFYFQNYVDAWTRGNMAVFFKNSAINTFVALFFIVIFAVTVGFAVTKMDWK
                     LKGTVAKYFQLGIMVPVATALIPLFQIYNKFHLINTRTSLILAYIAFGLSLSVYLITG
                     YLRSLPDEIMEAAVIDGCGIYKLMWYVVVPLMKNGIITVLVLQFFFKWNDLIFSMTFI
                     SDSKLKTIQTGLLYFQDEFGAKNWGAIFASVSMSVIPLLIIYMILNKAVIEGMTAGAV
                     KG"
     gene            1330..>1568
                     /locus_tag="PLU43_09740"
     CDS             1330..>1568
                     /locus_tag="PLU43_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="HOO28736.1"
                     /translation="MKIALVYTGTTPELEKMVQEEVKKNLGGDVEILSYADASILAEI
                     RGAGYVTANAAAKLVTLFMEAIENGADAILNLCSSV"
BASE COUNT          417 a          276 c          395 g          480 t
ORIGIN      
        1 cttgaaaaat atgtacaggc gtggatcggg caggcttcaa cggcgctgtg ggcactgtgt
       61 gttatggaca tctgggtggc catgggattt tattccgtta ttttttacgg ggcgcttctt
      121 gacattccgg gtgatgtcat tgaggcggcc aggatagacg gctgcaatgg gttcaattta
      181 ttccggtatg tattatcacc gcttataaaa ccggtcacga tcactgcact tgttttcagt
      241 ttttcaggaa ccgtcaagat gtttgaatcg gccacggctc tgaccggcgg aggtcccggc
      301 aatgccacga aatcgttgtc catgtacatg tataatgtag cattttccta taataaagta
      361 ggctatggca gcgtgatcgc actggtcatt tttttgatct gtattgcagg ttcctttgtc
      421 attaatttgt ttgacagaca gaaagactga gaggaggggc agaaatgaaa gcatggaaaa
      481 aaacgaaagc ctggctggtc agaattttga tttttttgtt gtgtatggtt gaaatctatc
      541 cgatattctg gatgctgaca gcttctttaa aaaaacagtc cgaatggaca tcaaaacccg
      601 cctatgcgtt aaatgaagga ttttattttc agaattatgt cgatgcatgg acaagaggca
      661 atatggctgt cttttttaaa aacagtgcca taaatacgtt cgttgctctg ttctttattg
      721 ttatttttgc cgtgacggta ggatttgcgg taacaaaaat ggactggaaa ttaaaaggaa
      781 cggtcgcgaa atattttcag cttggaatca tggttccggt tgccactgcg ctgattccgc
      841 tgtttcagat ctataataag tttcatctga tcaatacgag aacaagcctg attttagcat
      901 atattgcgtt cggtctgtcg ttatccgtgt atctgatcac cggatatctg cggtcattgc
      961 cggatgagat catggaagcg gcagtgatcg acggctgcgg gatctataag ctgatgtggt
     1021 atgttgtcgt tccgctgatg aaaaatggaa ttattacggt acttgttctg cagtttttct
     1081 tcaaatggaa cgatctgatt ttctcaatga catttatcag cgattcaaag ctgaaaacaa
     1141 tacagaccgg attattatat ttccaggatg agttcggagc caagaactgg ggcgctattt
     1201 ttgcatctgt gtccatgagt gtgattccgc ttcttatcat ttatatgatc ttaaataaag
     1261 ccgttattga aggaatgacg gcaggggctg taaaaggctg agcacgattt aagaacagga
     1321 ggctgcggaa tgaagatcgc acttgtatat acggggacca cgccggaatt agaaaaaatg
     1381 gtacaggaag aggttaaaaa aaatcttggg ggcgatgttg agattttatc gtatgcggat
     1441 gcttccattt tagcagaaat cagaggggcc ggttatgtaa cggcgaatgc tgccgcaaag
     1501 cttgtgactc tttttatgga ggcaatagaa aacggtgccg atgcaatttt aaatttatgt
     1561 tcttctgt
//
LOCUS       DAOSYE010000607         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70238_length_1568_cov_3.147389, whole genome shotgun sequence.
ACCESSION   DAOSYE010000607 DAOSYE010000000
VERSION     DAOSYE010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70238_length_1568_cov_3.147389"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLU43_09745"
     CDS             complement(<1..202)
                     /locus_tag="PLU43_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOO28737.1"
                     /translation="MYITKPLLEWFEENRRDLPWRTEPTPYHVWVSEIMLQQTRVEAV
                     REYYARFLKRLPKIRDLADAEEE"
     gene            complement(217..753)
                     /locus_tag="PLU43_09750"
     CDS             complement(217..753)
                     /locus_tag="PLU43_09750"
                     /inference="COORDINATES: protein motif:HMM:NF019481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HOO28738.1"
                     /translation="MREEPKEFQVYRHFRGTYYQVLSVATHTETGERLVVYRSLYEPA
                     EIYARPLEMFMSEISYKLYPDALNRRYRFELVTGDAKEPELREKMSFRQITRTEYEDY
                     DESVIDPDLEKFLDEKSYEKKLDLLMYLRKKADDEMLNTIAMSLDLELTRTTTEEKYE
                     EVFECLKTLGKYECNRLR"
     gene            complement(923..1339)
                     /locus_tag="PLU43_09755"
     CDS             complement(923..1339)
                     /locus_tag="PLU43_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28739.1"
                     /translation="MKRIIIKITSVFLLFCTLTYITVNAAAGDEETTCLTTEQYQQME
                     KDSVQTIKEVLSAHCFSDSGVMLTKENRDGKLYCYHIQIHHGRFAGLEKEEKEMLKCL
                     IEQSLQDVWEIPVEAYFTSEDAAGLLDCTFFPSTVS"
     gene            complement(1440..>1568)
                     /locus_tag="PLU43_09760"
     CDS             complement(1440..>1568)
                     /locus_tag="PLU43_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOO28740.1"
                     /translation="SAAGVKIGSIFGSKYKAKAELGGGIILLLIGIKILLNGLNII"
BASE COUNT          412 a          400 c          250 g          506 t
ORIGIN      
        1 cttcttcctc tgcatcggca agatccctga tctttggaag ccgttttaaa aatcttgcat
       61 aatactccct gactgcctct acccttgtct gctgcagcat gatttccgat acccatacat
      121 gatatggcgt cggttccgta cgccacggaa gatcccgcct attttcttca aaccattcca
      181 gcagcggctt tgttatatac atcagccgct cctcttctag cgcagcctgt tgcattcgta
      241 tttccccagt gtcttaaggc attcaaagac ttcctcatat ttctcttcgg tcgtcgttct
      301 tgtaagctcc agatccagcg acatggcaat tgtattcagc atttcatcat cagctttttt
      361 tctaagatac atcagcagat ccagcttttt ttcataagat ttctcgtcca gaaatttttc
      421 aagatccggg tcaatgaccg actcatcgta atcttcatac tccgtacgcg tgatctgccg
      481 aaatgacatc ttttctctca gctccggttc ttttgcatcc ccggtcacaa gctcaaaacg
      541 gtatcttctg ttcagagcat ccggatacag cttatagctg atctcactca taaacatctc
      601 aagcggcctc gcatagatct cggccggctc ataaagagaa cgataaacaa caagccgttc
      661 tccggtctcc gtatgcgtgg caaccgaaag cacctgataa taagtccccc taaaatgcct
      721 gtacacctga aattcttttg gttcttctct cattttcaca ctccctatgc tgctggactt
      781 agctttaaaa caaatcattc acagcttatt tgttttaagt tcgacatgta catagtataa
      841 tccactttag gagatttgtc attgatttat caaatatttt acatttgtat catgtgttat
      901 ttaataataa gacagcccct gttcaggaaa cagtgcttgg gaaaaaagta cagtcaagta
      961 acccggccgc atcctccgat gtgaaatatg cctcaacggg tatctcccac acatcctgaa
     1021 gagactgctc tatcaaacat ttaagcattt ccttttcttc tttctccaga ccggcaaatc
     1081 ttccgtgatg tatttgaata tggtagcaat agagcttccc gtccctgttt tccttcgtta
     1141 acataactcc actatcagaa aagcagtgcg cagacaatac ttcttttatt gtctgtaccg
     1201 aatccttttc catctgctga tactgctccg tggtcagaca tgttgtttcc tcgtctcccg
     1261 ctgctgcatt tactgttata tatgtcagcg tacagaacag caaaaaaaca cttgttatct
     1321 ttataattat ccttttcatt ttttacgcct ccgtttttta gtaaacaaaa ttaatataga
     1381 ctttattgac aggttgttta aaaccagtct atatcttttc aagtactaaa aaacggactt
     1441 taaataatgt ttaacccatt cagcaaaatt ttaattccaa tcaaaagcaa aatgatcccg
     1501 ccgcccagct ctgcctttgc tttatatttc gaaccgaaaa tactgccgat tttaacaccg
     1561 gctgcaga
//
LOCUS       DAOSYE010000608         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70340_length_1567_cov_2.250661, whole genome shotgun sequence.
ACCESSION   DAOSYE010000608 DAOSYE010000000
VERSION     DAOSYE010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70340_length_1567_cov_2.250661"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /gene="rlmD"
                     /locus_tag="PLU43_09765"
     CDS             <1..1419
                     /gene="rlmD"
                     /locus_tag="PLU43_09765"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOO28741.1"
                     /translation="AGEGMGRVNGYTLFVKDAVIGDQAEVKLVKVKKRYAFARLETLL
                     SASPYRVDPPCPIHRQCGGCQIQALSYAEQLRFKEKKVQNHLIRLGGFQEAEIRQLME
                     PVIGMEERESERGQRGFGYRNKAQFPVGRDKEDRLIIGFYAGRTHSIIPNTDCLLGLS
                     INAPLLKTILSYMEQAGISAYDETTGTGLVRHILLRAGMVTGEVMVCLVINGKKLPQD
                     EKLVESLLQCANDSWGSRQTNEPFYKITHISFCVNTENTNVIMGNEIHLLYGKPYIRD
                     MLHGLSFHISPLSFYQVNPVQAEKLYAAVLAFAALTGTETVWDLYCGIGTISLCMARN
                     AKQVYGVEIIPQAIADAKENAARNQITNAEFFAGKAEEILPRYYTTHSGSSSDDLEGA
                     MLHPDVIVVDPPRKGCDMACLETMLKMEPDRIVYVSCDSATLARDLKFLCSDAYELKR
                     IRPCDMFCQTVHVEVVTLLSKK"
BASE COUNT          415 a          344 c          424 g          384 t
ORIGIN      
        1 gcaggagagg gtatgggcag agttaacggc tatacccttt ttgtaaagga tgccgtaatc
       61 ggagatcagg cggaagtaaa gcttgtaaaa gtgaaaaaga gatacgcgtt cgcaagactg
      121 gaaacccttc tttctgcttc gccttaccgt gtagacccgc cctgtccgat ccacaggcaa
      181 tgcggcggat gccagatcca ggcgctcagc tatgcagaac agctgcgttt taaggagaaa
      241 aaagtacaaa atcatctgat ccgtctcgga ggcttccagg aagcagagat caggcagctc
      301 atggagccgg taatcggtat ggaggaacgg gagtcggaac gaggtcaaag gggatttggc
      361 tatcgcaata aggcccagtt tccggtaggc agggataagg aagaccggct gatcatcgga
      421 ttttatgccg gcaggacgca cagtatcatt ccgaacacgg actgcctgct ggggctttcc
      481 ataaatgccc cgctcttaaa aacgatcctt tcctatatgg agcaagcggg tatttccgca
      541 tatgatgaga caacaggaac cgggctcgtg cggcatattc tgctccgcgc cggcatggtg
      601 accggtgaag tgatggtctg tcttgtaatc aacggaaaga agctgccgca agacgagaag
      661 cttgtagaaa gtcttttgca gtgtgcaaat gactcatggg gcagcaggca gacaaacgag
      721 cctttttaca agatcacgca tatttcgttt tgcgtcaata cggaaaatac aaatgtcatt
      781 atgggaaatg agatccatct gctttacgga aagccctata tccgtgatat gctgcatggc
      841 ctctcttttc atatttcccc gctgtctttt taccaggtca acccggtaca ggcagagaag
      901 ctgtatgcag ccgtacttgc gtttgctgca ctgaccggaa cagaaacggt atgggacctt
      961 tactgcggga tcggtacgat ctctttgtgt atggcgcgta atgcaaagca ggtctatggt
     1021 gtcgaaataa ttccgcaggc gatcgcagat gcaaaggaaa acgctgcccg caaccagatc
     1081 actaatgcgg aattttttgc aggaaaggca gaggagatcc tgccccggta ttataccacg
     1141 catagcggca gcagttcgga cgatttggag ggcgcgatgc tccatccgga tgttattgta
     1201 gtggacccgc cgcgcaaggg ctgcgatatg gcctgtcttg aaacgatgct gaaaatggag
     1261 ccggaccgga tcgtttatgt cagctgtgac agcgctacgc tcgcgcggga tcttaaattt
     1321 ctgtgcagcg atgcctatga attgaagcgg atccgtccat gtgatatgtt ttgccagacc
     1381 gtgcacgttg aggtggtcac tttgctgtca aaaaagtaga aaattactga tatattagtt
     1441 atttgagaag ttattggcag aagaaaaatg aagaatattt gaatgcgttt tgatcatatg
     1501 tatatgtatc gtttcaattg ttcgtagaaa ttccctcggg ttccagccat aattacaact
     1561 acaactt
//
LOCUS       DAOSYE010000609         1565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70479_length_1565_cov_2.774834, whole genome shotgun sequence.
ACCESSION   DAOSYE010000609 DAOSYE010000000
VERSION     DAOSYE010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70479_length_1565_cov_2.774834"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLU43_09770"
     CDS             complement(<1..939)
                     /locus_tag="PLU43_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28742.1"
                     /translation="MEARPSVLDQLKKQNLFEWYYILVMCGYLLTLVTDAMRPGVFAA
                     ILLLGVAAELVLRGRVSFRHMIDMLAAAYFIYNLLSVIWLTRNGFPADIYAEEFVSSI
                     LPMVFYMVGKSAGQLTEKFYKKYIAALLLIGAAGFLLYVFAPQFYLDYLVKWSYISKA
                     DAPTMRIRMHSVIGSTILGFLSVCGMLASSWFVMQKKERLKGAVLFAVCFLLAMLSNQ
                     RSAMVAAILVVLYINHLIFFEFRLVSKKYFIGECGLIAAAFLGLCICCFDVVLKIYYR
                     LISLPGAIGQRSEQWVAAVNNMFSSWLGNGLGANGHK"
     gene            complement(965..>1565)
                     /locus_tag="PLU43_09775"
     CDS             complement(965..>1565)
                     /locus_tag="PLU43_09775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO28743.1"
                     /translation="DLFIDSGRPGALRYDQFFFPSGNILYKNHACIGKAVQLLREKGI
                     TDFEVVLTLTEEEAADVMELDKNDHNIVCRGRLSRNEVYRLYQERILLFPSYIETFGY
                     PPAEARKAGTVIFASDCPFCHEVLENYEDAYYFDPFQPKELAALMEQSIKGELVRRAP
                     AGRKFEYAVPACGKQSSWSTVCEQIAAAAKSDSTCAGMP"
BASE COUNT          457 a          423 c          323 g          362 t
ORIGIN      
        1 tttatgccca ttggctccga gtccgttgcc aagccagctg ctgaacatat tgttaacggc
       61 tgccacccac tgctcactgc gctgcccgat cgcacccggc agagaaataa gacgataata
      121 tattttcagg accacgtcaa agcagcagat acaaagtccc agaaaagccg ctgcgatcag
      181 accgcattcc cctataaaat attttttgga aacaaggcga aattcaaaga agatcaaatg
      241 gttgatataa agcacaacaa gaatcgctgc gaccatggcg gaacgctgat tactgagcat
      301 ggccaataaa aagcatacgg caaaaagcac ggctcctttc agccgttctt ttttctgcat
      361 cacaaaccag gaagacgcca gcataccgca aacagaaagg aatcccaaaa ttgtgctgcc
      421 gatgaccgaa tgcatgcgga tccgcatcgt cggtgcatcc gcctttgaaa tataactcca
      481 tttcacaaga tagtccagat aaaactgcgg cgcaaataca taaagcagaa aaccggcagc
      541 gccgatcagc agcagagctg cgatatattt tttataaaac ttttcggtaa gctgcccggc
      601 agacttccca accatgtaaa acaccatggg gagtatggag ctgacaaact cttcagcata
      661 aatatccgca ggaaatccgt tcctcgtcag ccagatgacg gacagcaggt tatagataaa
      721 ataagccgcc gcaagcatgt cgatcatatg ccgaaaggaa acacgccccc tcagcacaag
      781 ctccgccgcc acaccaagca gcagtatggc tgcaaaaact ccaggacgca ttgcatccgt
      841 tacaagcgtc agcaggtagc cgcacatgac aagtatataa taccactcaa acagattttg
      901 ttttttcaat tgatccaata cagaaggtct cgcttccatt tcggtccctc actttatttt
      961 acggttaagg catgcccgca cacgtactgt cggattttgc agctgctgct atttgttcac
     1021 agactgtact ccagctgctt tgctttccgc acgcaggaac agcatattcg aatttccgcc
     1081 cggcaggcgc ccggcgcact aattcaccct tgatcgattg ttccatcagt gctgccagtt
     1141 cctttggctg aaacggatca aaatagtatg cgtcctcata attttcaagc acttcatgac
     1201 agaatggaca gtcagacgca aaaataacgg ttcccgcttt tctcgcttct gccggcggat
     1261 agccaaacgt ctcaatataa gaaggaaaca gcagaatcct ttcctgatag aggcggtata
     1321 cttcattccg cgaaagccgg cccctgcaga cgatattgtg atcatttttg tcaagctcca
     1381 ttacatccgc cgcttcctct tctgtaagcg tcagtactac ctcaaaatca gttatgccct
     1441 tttcccgcag caattgtacg gctttgccga tacaggcatg atttttatac aaaatatttc
     1501 cggatggaaa aaagaactga tcataccgca atgcccccgg ccggccggaa tcgataaaca
     1561 gatca
//
LOCUS       DAOSYE010000610         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70534_length_1564_cov_3.207422, whole genome shotgun sequence.
ACCESSION   DAOSYE010000610 DAOSYE010000000
VERSION     DAOSYE010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70534_length_1564_cov_3.207422"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1564
                     /locus_tag="PLU43_09780"
     CDS             15..>1564
                     /locus_tag="PLU43_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOO28744.1"
                     /translation="MRKQEEDALRNNDSLPSVCFPTASAIACSWDIEAAAKLAKALGT
                     EAVKEQIAVVLGPGINIKRSPLCGRNFEYFSEDPYLAGTLATAYINALQSTGVAASLK
                     HFAGNSQETHRQTSNSQIDERALREIYLSAFEMAVKEARPATIMASYNRLNGSYACEN
                     KMLLTDILRKEWGFEGVVVSDWGACADLPKCIEAGMDLEMPDSCNYHSPMLEEALQSG
                     KLSEVSIDRAAGNMLHLTDRYSGSSGTLKVSDHTDLHQLAKELACESAVLLKNDGVLP
                     LREESIILVIGDLAKTMRFQGGGSSHINTVKTPNALEALRANGVKLQFYRGYRADSDR
                     VDQKLEKEAIDAVKAAEGETPVLFFGGLTEYTEGEGYDRKTLDLPKNQVSLVEKLHVL
                     GAKLAFVSFGGSPFSIPFISHVHAILHMYLGGEAAGEACTQLLLGNVNPSGKLAETFP
                     MKTEDIPCNPWFGTGSDDVEYRESIFVGYRYYDTYQRPVLFPFGHGLSYTTFRYSNLE
                     ADCDTFCGG"
BASE COUNT          408 a          334 c          460 g          362 t
ORIGIN      
        1 acggctcgca tggattgaga aaacaagagg aagatgcgct gcgtaataac gatagtcttc
       61 catcggtctg ttttccgaca gcaagcgcga ttgcctgcag ctgggatata gaagcagctg
      121 caaagcttgc aaaagcgctt ggaacagaag cggttaaaga gcagatcgcc gttgtgcttg
      181 gcccgggaat caatatcaag cgttccccgc tgtgcgggcg gaattttgag tatttttcag
      241 aggatccgta tctggcagga acgcttgcga cagcgtatat caacgccctg cagagtactg
      301 gagttgctgc ttccctgaag cattttgcag gcaacagcca ggaaacgcac aggcagacat
      361 caaacagtca gatcgatgag cgggcactgc gggaaattta tctttcagca tttgaaatgg
      421 ctgtgaagga agcaaggccg gcgaccataa tggcatctta taatcggtta aacggcagct
      481 atgcctgcga aaataaaatg ctgctgacag atattctgcg gaaggaatgg gggtttgaag
      541 gcgttgtcgt atcggactgg ggtgcatgcg cagatttgcc caagtgtatc gaagccggga
      601 tggatctgga aatgccggac agctgcaatt atcacagccc tatgctggaa gaggcgctgc
      661 aaagcggtaa attgagcgaa gtgtcgattg atcgggcagc aggaaatatg ctgcatttaa
      721 cagaccggta ttctggaagc agcggaaccc tgaaagtgtc ggatcatacg gatctccatc
      781 agcttgcaaa agaactggcc tgcgaatcgg ccgtactgtt aaaaaatgat ggggttttgc
      841 cgctgcggga ggaatcgatc atactagtca ttggagacct tgcgaaaacg atgcggtttc
      901 agggcggcgg aagcagccat atcaataccg tgaagacacc aaatgccctg gaggcgctgc
      961 gggcaaacgg ggtgaaactg cagttttaca gagggtaccg tgcagacagt gaccgggttg
     1021 atcagaagct tgagaaagaa gccatagatg cggtcaaggc tgcagaaggc gaaacgccgg
     1081 tgcttttttt cggcggactg acggaatata ccgaagggga aggatatgac cggaaaacgc
     1141 tggaccttcc aaaaaatcag gtcagcctgg ttgaaaaact gcatgttttg ggtgcaaagc
     1201 tggcatttgt atcctttggc ggttctccgt tctctattcc ttttatttca catgtgcatg
     1261 cgatcctgca tatgtatctt ggcggagagg cagcaggtga agcgtgcaca cagctgctgc
     1321 ttggaaacgt gaatccaagc ggaaagcttg cggagacatt tcctatgaag acggaagata
     1381 ttccatgcaa tccatggttt gggaccggca gtgatgatgt agagtatcgg gagagtattt
     1441 ttgtcgggta tcggtattat gatacctatc agcggccggt gctctttccg tttggtcacg
     1501 gactgtccta tacaaccttc cggtattcta accttgaagc ggactgcgat acgttttgcg
     1561 gcgg
//
LOCUS       DAOSYE010000611         1561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_70782_length_1561_cov_2.506640, whole genome shotgun sequence.
ACCESSION   DAOSYE010000611 DAOSYE010000000
VERSION     DAOSYE010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70782_length_1561_cov_2.506640"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="arcC"
                     /locus_tag="PLU43_09785"
     CDS             complement(<1..726)
                     /gene="arcC"
                     /locus_tag="PLU43_09785"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOO28745.1"
                     /translation="MMKKKNRIVIALGGNALGNTLPEQMKAVRSTSRSVVDLIEAGNE
                     VVISHGNGPQVGMINNAMAALSREDKDQPNTPLSVCVAMSQAYIGYDLQNALREELKN
                     RGLSIPVTTMITQVRVDENDQAFSHPDKPIGHFMTKEQADLAHEKYGHLMREDAGRGY
                     RRVVPSPKPAEIVEIDAIRTLVNSGSVVIACGGGGIPVVLHGNHLKGMSAVIDKDFAS
                     CKLAQELDADFLIILTAVEHAAIH"
     gene            complement(754..>1561)
                     /gene="ygeW"
                     /locus_tag="PLU43_09790"
     CDS             complement(754..>1561)
                     /gene="ygeW"
                     /locus_tag="PLU43_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOO28746.1"
                     /translation="GNTYMHHVSEAVREGYADGVLEQIPTLVNLQCDIDHPTQCMADM
                     LHIIHEFGGVENLKGKKLAMSWAYSPSYGKPLSVPQGVIGLMSRFGMDIVLAHPEGYE
                     IMPETIEVARKNAALSGGSFTKTNNMEEAFANADIVYPKSWAPFAAMETRTDLYGNGD
                     FDGIDKLEKELLAQNASHKDWTCSEELMKTTKDGKALYLHCLPADISDISCTAGEVDA
                     SVFDRYRTPLYKQASFKPYIIAAMIFLSKFEDPAALLQTLESNGIKRKKA"
BASE COUNT          352 a          391 c          378 g          440 t
ORIGIN      
        1 atggatcgca gcatgttcga ctgcagtcag gatgatcaga aaatcagcgt ccagctcctg
       61 tgccagtttg cagcttgcaa aatctttatc aatgactgca ctcatacctt ttaaatgatt
      121 gccatgcagt acgacaggga tcccgccgcc gccgcatgcg atcacaacac tgccggaatt
      181 gacgagtgtc cggatagcgt cgatctcgac gatctcggcc ggttttggag acggtactac
      241 acggcggtag ccgcgtcccg catcttccct cataagatgg ccatattttt catgggccag
      301 atcagcctgt tcttttgtca taaagtgacc gatcggtttg tccggatggg aaaaggcctg
      361 gtcattttca tcgacacgga cctgtgtgat catcgttgtg acagggatgc tcagtccgcg
      421 gttcttgagt tcttcgcgca gcgcattctg cagatcatag ccgatatatg cctgactcat
      481 tgcaacacag acagaaaggg gcgtattcgg ctgatctttg tcttcacggg ataatgccgc
      541 catggcgtta ttgatcatac cgacctgcgg accatttccg tgagaaatga ctacttcatt
      601 gcctgcttct atcagatcga ctacggaacg ggaagtactc ctgaccgctt tcatctgttc
      661 gggaagcgtg ttgccaaggg catttccgcc caaggcaatc acaattctat tttttttctt
      721 cataatactc tctcttccga aaaagttttc cggctacgct ttctttcttt tgattccgtt
      781 gctttcaagt gtttgcaaaa gagctgccgg atcttcaaat ttacttagga aaatcattgc
      841 ggctataatg tacggcttaa agcttgcctg cttatagagc ggtgtgcggt agcggtcgaa
      901 tacggaagca tccacttctc cggccgtgca gctgatatcg gaaatatctg caggcagaca
      961 atgcagatag agtgccttgc cgtcctttgt tgttttcatt agttcttcgc tgcatgtcca
     1021 gtctttatgg gaagcattct gggcaagcag ctctttttcg agtttatcga tgccgtcaaa
     1081 gtcaccgttg ccgtatagat cggtacgtgt ttccatggca gcaaatggtg cccagctttt
     1141 cggatagacg atatcagcat ttgcaaaggc ttcttccata ttatttgttt ttgtgaaaga
     1201 gcctccggat aaagcggcat ttttcctggc gacttcgatc gtctcaggca tgatttcata
     1261 tccttccgga tgtgccagta cgatatccat gccgaaacgg ctcatcagac cgatgacacc
     1321 ctgcggaacg gaaagcggtt tgccgtagga aggagaatat gcccaggaca tggccagctt
     1381 tttgcccttc agattttcaa caccgccgaa ttcatgaatg atgtgcagca tatctgccat
     1441 acactgtgtc ggatggtcga tatcgcactg caggttgaca agcgtcggga tctgctcaag
     1501 aacaccgtcc gcatagcctt cccggacggc ttcggataca tggtgcatat acgtattgcc
     1561 t
//
LOCUS       DAOSYE010000612         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71213_length_1555_cov_3.418667, whole genome shotgun sequence.
ACCESSION   DAOSYE010000612 DAOSYE010000000
VERSION     DAOSYE010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71213_length_1555_cov_3.418667"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLU43_09795"
     CDS             <1..250
                     /locus_tag="PLU43_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOO28747.1"
                     /translation="VKGESMINAGIFDGDQIFVEQCNTARNGDMVVALIDDSATVKTY
                     YKENGHYRLQPENDNMEPIIVEHVDILGKVYGVFRFMN"
     gene            complement(313..666)
                     /gene="rsfS"
                     /locus_tag="PLU43_09800"
     CDS             complement(313..666)
                     /gene="rsfS"
                     /locus_tag="PLU43_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOO28748.1"
                     /translation="MDESKKMAQIAIDALKDKKAEDIKIIDIGKISVLADYFIIATGS
                     NRNQIQAMTDHVQEKLYKEAECSPKQIEGYETANWILMDYRDVVIHVFDRENRSFYDL
                     ERIWRDGKLMEELEL"
     gene            complement(659..1282)
                     /gene="yqeK"
                     /locus_tag="PLU43_09805"
     CDS             complement(659..1282)
                     /gene="yqeK"
                     /locus_tag="PLU43_09805"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOO28749.1"
                     /translation="MVEFSKNKANDIQKLRKKVKRKLDKKRYIHTLGVSYTAAALAMR
                     YGADIEKAQVAGILHDCAKYLECGEMLGLCEKYHISITEVERHNPCLLHAKLGGFFAM
                     KKYHVHDKEIIGAIINHTTGRPNMNLLEKVIFVADYIEPSRDRVPNLKEIRRLAFVEI
                     DEAVIFILKDTLCYLSSKQVEVDDMTQKTYNYYEKILEKKKENQLDG"
     gene            complement(1301..>1555)
                     /locus_tag="PLU43_09810"
     CDS             complement(1301..>1555)
                     /locus_tag="PLU43_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HOO28750.1"
                     /translation="PQLIFDHCILVAAPRYYISDEALLEQKRHLEKLYHARIRLLHTP
                     NIDISSNMIRARIADKKSICYYVPKDVETYIYKKKLYRMK"
BASE COUNT          444 a          343 c          249 g          519 t
ORIGIN      
        1 agtaaagggt gaaagtatga tcaatgccgg gatctttgac ggagatcaga tttttgtcga
       61 gcaatgcaat acggcacgca acggagatat ggtcgtggca ttgatcgatg attctgccac
      121 tgtaaaaact tattataaag aaaacggaca ttaccgcctt cagccggaaa atgacaatat
      181 ggaaccgatc attgtggaac atgttgatat tctcggcaag gtttatgggg tcttccgctt
      241 tatgaattaa tctatttcat aaaaagacag ccaggtattt atataccggc tgtcttttta
      301 taacacagaa ctctacagtt ccagctcttc catcaatttt ccgtcacgcc aaatgcgttc
      361 caaatcataa aatgaacgat tctctctgtc aaaaacatga ataacaacat cccgataatc
      421 catcaggatc caattggcag tttcatatcc ttcgatctgc tttggagaac attctgcttc
      481 tttatacagc ttttcctgta catgatcagt catcgcctgt atctgattac gattggaacc
      541 ggtcgctata ataaaataat ctgccaaaac actgattttt ccgatatcga ttatttttat
      601 atcctctgcc ttcttatctt tcaatgcgtc gattgctatc tgcgccattt tttttgattc
      661 atccatctaa ctgattctcc ttcttttttt caagaatctt ttcataatag ttatacgtct
      721 tctgcgtcat gtcatcaact tccacctgtt tggaggaaag atagcaaagc gtatccttca
      781 aaataaaaat aaccgcttca tcaatctcta caaatgccag cctgcggatc tccttcagat
      841 ttggcactct atccctgctc ggctctatat aatctgcaac aaaaatcact ttttccagca
      901 gattcatatt aggacgccct gtcgtatgat tgataattgc cccgataatt tccttgtcat
      961 gcacatgata ctttttcatt gcaaaaaagc caccaagctt agcgtgcagt aaacatggat
     1021 tatgccgctc tacttccgta atactgatat gatatttttc acacagtcca agcatttcac
     1081 cgcattccag atatttggca caatcatgca aaattccggc aacctgtgct ttttcaatat
     1141 cagctccata tctcatggca agagctgccg ccgtgtatga aactcctaac gtatgaatat
     1201 atcttttttt atccagtttc ctctttactt ttttccgaag tttttgaata tcatttgctt
     1261 tattttttga aaactcaacc attggtaacc ttctttataa ctatttcatt ctatatagct
     1321 tttttttata aatatatgtt tccacatctt ttggaacata ataacagatt gattttttat
     1381 ctgcgatcct ggcacgtatc atattggaag aaatatcaat attgggcgta tgcagcagcc
     1441 tgattctggc atgatacagc ttttcaagat gcctcttctg ttcaagaagc gcttcatccg
     1501 atatataata acgcggtgcc gcaacaagaa tacaatgatc aaaaataagc tgcgg
//
LOCUS       DAOSYE010000613         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71350_length_1553_cov_4.333111, whole genome shotgun sequence.
ACCESSION   DAOSYE010000613 DAOSYE010000000
VERSION     DAOSYE010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71350_length_1553_cov_4.333111"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLU43_09815"
     CDS             complement(<1..1137)
                     /locus_tag="PLU43_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO28751.1"
                     /translation="MKVKTLTFFSNYFNHHQKALCDEWSALLGAGFTFVETEPIEGFR
                     ASMGWGEKEYPSYVLRTYADEASRQTALRLGLESDVVVMGTAPEFYIEARLAANKLTF
                     RYSERPLKEGFIKFFIPRLTKKYIRLHIRNRKKPLYILGASAYTAQDYKKMRSYLGKC
                     YKFGYFPKHIDYEPDQLTARKKEKAGSPEMTTILWEGRMLKLKRPDLVIKAASRLKRK
                     GYQFHVTMVGEGEERMRTERLAEKYGLSGIVSFENFLSPAEARERMADAKVYVMTSNF
                     WEGWGSVIYEALNAGCAVVASHACGATPWLVEHEKTGLVFKSGSVKSLTEQLEKLLKQ
                     PSLQEVYGTAAYGQMHRLWNPKTAAGNVLTLAECLSEGKTSPFEK"
     gene            complement(1165..>1553)
                     /locus_tag="PLU43_09820"
     CDS             complement(1165..>1553)
                     /locus_tag="PLU43_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO28752.1"
                     /translation="FFADLRMHLPNKKTFVKTARNRKYATSRDWNHPTTFITKQMYET
                     YHYKNDTLHDDYDLILRIKKAGAHIEIVNRTLANFRMNGVSHERSVSGAWHRAKIKYG
                     IYRQNGYSPLYFFECVAVEAAKLIIG"
BASE COUNT          355 a          426 c          319 g          453 t
ORIGIN      
        1 tttctcaaac ggcgatgtct ttccttccga taagcattcc gccagtgtaa gtacgttccc
       61 ggcagctgtt ttcgggttcc acagcctgtg catctgccca tatgccgctg ttccgtatac
      121 ttcctgcagg gacggctgct taagcagttt ttcaagctgt tctgtcagac tcttcacact
      181 gccgctttta aaaacgagtc cggtcttttc gtgttcaaca agccacggcg tggccccgca
      241 cgcatgactt gccacgaccg cacagccggc attgagtgct tcgtagatca cagaacccca
      301 gccttcccag aaattgcttg tcatcacata gacctttgca tccgccatcc gttctctggc
      361 ctctgctggt gataaaaaat tctcaaacga tacaataccg gaaagaccgt acttttctgc
      421 cagccgttcc gtacgcatgc gctcttctcc ttcaccgacc attgtcacat ggaattgata
      481 gccttttctt ttcaggcggg aagctgcttt gattacaaga tccggacgct tcagcttcag
      541 catgcgtcct tcccataaaa tagtcgtcat ttccgggctg ccggcctttt ccttctttct
      601 tgcagtcagc tgatctggct catagtcaat atgctttgga aaatacccaa atttataaca
      661 tttcccaaga tagctgcgca tttttttata atcctgcgca gtataagcac ttgcgccaag
      721 aatataaagc ggctttttcc ggttcctgat atgcagacgg atatattttt ttgtcagtct
      781 cggaataaaa aacttgataa atccttcctt gagcggcctc tccgaatagc ggaacgtcag
      841 cttatttgct gcaagacggg cttcaatata aaactccggt gccgttccca ttacaacgac
      901 atcactttca agtccgagcc gtaatgctgt ctgccgggat gcttcgtccg catacgtgcg
      961 cagcacataa gacggatatt ccttctctcc ccagcccatg ctcgcgcgga acccttcgat
     1021 cggctccgtt tcgacaaatg taaacccggc ccctaaaagc gcagaccatt cgtcacacag
     1081 cgccttctga tggtgattaa aataattcga aaaaaaagtc agtgtcttta ctttcattgt
     1141 ccagttccct taccttcggc catctcagcc tatgatcagc tttgcagctt ccacggccac
     1201 acattcaaaa aaataaagcg gcgaataacc gttctgtctg taaattccgt atttgatctt
     1261 tgcacggtgc catgcgccgg acacgcttct ttcatggctc acaccgttca tgcggaaatt
     1321 ggcgagcgtc cggttgacga tctcaatatg cgcacccgct ttttttatcc gcaggatcag
     1381 gtcatagtcg tcatggagcg tgtcgttttt ataatgatat gtttcataca tctgttttgt
     1441 aataaatgtc gtcgggtggt tccagtcgcg tgatgtcgca tatttccggt tccttgccgt
     1501 cttcacaaat gtctttttgt tcggcagatg cattctaaga tcggcaaaaa aaa
//
LOCUS       DAOSYE010000614         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71439_length_1552_cov_2.732131, whole genome shotgun sequence.
ACCESSION   DAOSYE010000614 DAOSYE010000000
VERSION     DAOSYE010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71439_length_1552_cov_2.732131"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLU43_09825"
     CDS             <1..479
                     /locus_tag="PLU43_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOO28753.1"
                     /translation="IEDPYFRSHIKGAKAAGLKVGVYFFTTAITETEAVEEASMCAAL
                     CSGYGINYPVFMDVESNNRSGFNSLSASQRTTIIKAFCSTVSSAGYTPGVYANKTWLT
                     SYMNASSLSSYKIWLAQYNSGGPTYSGRYDLWQYTSKGSVDGISGNVDMNQSYLGY"
     gene            504..1061
                     /gene="rfbC"
                     /locus_tag="PLU43_09830"
     CDS             504..1061
                     /gene="rfbC"
                     /locus_tag="PLU43_09830"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOO28754.1"
                     /translation="MSKITVETCHIEGLKVITPTVFGDKRGYFMETYNFNDFKEAGIG
                     QNFVQDNQSSSRKGVLRGLHFQIQYPQDKLVRVIRGEVFDVVVDMRKDSETYGQWYGV
                     RLSEENKKMFFIPKNFAHGFLVLSDFAEFTYKCTDFYHPNDEGGIIWDDKEIGIDWPI
                     ERDMELIISDKDTKWPTLKELKTGK"
     gene            1098..>1552
                     /locus_tag="PLU43_09835"
     CDS             1098..>1552
                     /locus_tag="PLU43_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28755.1"
                     /translation="MSKKKKIILLVSAFVLALNLYIIFHHNPLAERTQEIIKKVVSCV
                     ILDGIYLVFVVMYEKIVILPIELYQNRLLIWKLSKNDFKTRYAGSYLGIVWAFVQPVI
                     TVLLYWFVFTVALPSRAVAVKGDIEIPYILWLIAGIVPWFFFSEALSNGT"
BASE COUNT          447 a          265 c          385 g          455 t
ORIGIN      
        1 tgatcgagga tccgtatttc agatctcata tcaagggagc gaaggcagca ggactgaagg
       61 ttggtgtata cttctttacg acagcgatca ctgagactga ggcagtagaa gaggcaagta
      121 tgtgtgcggc actctgttcc ggctatggta tcaattatcc ggtatttatg gatgtggaga
      181 gcaacaatcg ttccggattt aattcattgt ccgcttcaca gcgtacaacg attattaaag
      241 ctttctgcag tacggtcagc tcagcaggct atacaccggg cgtctatgca aataaaacat
      301 ggctgacgag ttatatgaat gcctcgtccc tgtcgtcata caagatctgg ctggcgcagt
      361 acaattctgg aggcccgact tattcgggac gctatgacct gtggcagtat acttctaaag
      421 gaagcgtaga cggtatcagt ggaaatgttg atatgaatca gtcatatctt ggatattaat
      481 aaaagaaaag aaggaacggc aaaatgagca aaatcacagt ggaaacttgt catatcgaag
      541 gtctgaaggt gattacgcca acagtttttg gtgacaagcg cgggtatttt atggagacct
      601 acaattttaa tgactttaag gaagcgggta tcggccagaa ttttgtgcag gataaccagt
      661 cgtcttccag gaaaggagtt ttgcgtggtc tgcattttca aatacaatat ccgcaggata
      721 agcttgtcag ggtcattcgc ggggaagtgt tcgatgttgt agtcgatatg aggaaagact
      781 cagaaactta cggacagtgg tatggtgtca gactttcgga agaaaataaa aaaatgtttt
      841 ttattccgaa aaattttgca catggttttc tcgttttgtc ggactttgcg gaatttactt
      901 ataaatgtac ggatttttat catccgaatg atgaaggcgg tattatctgg gatgacaaag
      961 agatcggaat agactggccg atcgaaaggg atatggagct gatcatatcc gacaaagata
     1021 caaaatggcc gaccctgaag gaattaaaga ccggtaagta gtaaacaggt gtacagggtg
     1081 ttcctaaagg agagtttatg tctaaaaaga aaaaaatcat tctgcttgta tcagcatttg
     1141 tattggcgtt gaatttatat atcatttttc atcataatcc tttggccgag agaacacagg
     1201 aaattattaa gaaagtagta tcctgtgtaa tattggatgg aatctatctt gtttttgttg
     1261 tcatgtatga aaagatcgtg attcttccga tcgaattata tcagaaccgg cttttgatct
     1321 ggaagctgtc caaaaatgat tttaaaacaa gatatgcagg gtcctattta ggtattgtat
     1381 gggcgtttgt acagccggtc atcacagtgc ttctttactg gtttgtgttt acggtcgcgc
     1441 tgccgtcgag agcagttgcg gttaagggcg atatcgaaat cccgtatatt ttatggctga
     1501 tcgccggtat cgtaccatgg ttcttttttt cagaggcact ttcaaacggt ac
//
LOCUS       DAOSYE010000615         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71527_length_1551_cov_2.687834, whole genome shotgun sequence.
ACCESSION   DAOSYE010000615 DAOSYE010000000
VERSION     DAOSYE010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71527_length_1551_cov_2.687834"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLU43_09840"
     CDS             <1..364
                     /locus_tag="PLU43_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOO28756.1"
                     /translation="KDQTVVEICPEMLAGLSAPRPCAEIVDGIVTDSSGNSVDEIYKK
                     GVACALEKIKDMEFDLVVLQSRSPTCGVNQIYDGRFLGKLKEGQGLFARALIDAGYHV
                     VDIEDAEEFAGGKQKGLL"
     gene            364..615
                     /locus_tag="PLU43_09845"
     CDS             364..615
                     /locus_tag="PLU43_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase to dihydroxyacetone kinase"
                     /protein_id="HOO28757.1"
                     /translation="MLEFRYDTQLLIEGEELDEDIISQYFTDHFSGDCLLAVGDEELI
                     KIHFHTNEPWKVLEYCASIGEIYDIVVEDMERQSNGLHG"
     gene            730..1077
                     /locus_tag="PLU43_09850"
     CDS             730..1077
                     /locus_tag="PLU43_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO28758.1"
                     /translation="MNEDEIVSGLILELRRGTLILLVLSQLKEPKYGYYLVKTLQENG
                     IPIEANTLYPLMRRLETQGLLSSEWETSETKPRKYYRLTKDGEAVLEKATLHWKQFSA
                     DVNRLLEVEKDGK"
     gene            1067..>1551
                     /locus_tag="PLU43_09855"
     CDS             1067..>1551
                     /locus_tag="PLU43_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28759.1"
                     /translation="MENNLTERYIYAVTRRLPAKMKEDIAKEIRTLIEDMLEERCGII
                     SPTEKDVRVVLTELGTPDELYQTYSGSGEKCLIGPPYYAQYLFVLKLVLLCTAFGLTV
                     SLIVQLLTPNGAGPWYEILLQWFFTVFSGLGMGFAYVTLLFAFFYHKKIEFDTAFSLD
                     DL"
BASE COUNT          444 a          277 c          435 g          395 t
ORIGIN      
        1 aaaggatcag actgttgtgg agatctgccc ggaaatgctg gccggtctgt cggctccgcg
       61 cccctgtgcg gagatcgttg acgggatcgt gacggacagc agcggcaatt ctgtggatga
      121 aatttacaaa aagggtgttg catgtgcact tgaaaagatc aaagatatgg agtttgatct
      181 tgttgtattg cagtcaagaa gtcctacgtg cggggtaaat cagatttacg acggcaggtt
      241 tttgggaaaa ctgaaagaag gacagggatt atttgcacgg gcactgatcg atgccggata
      301 ccatgttgta gatattgagg atgctgagga gtttgccggc ggcaaacaga aaggattgtt
      361 ataatgttgg aattcagata tgatacacag ctgctgatcg aaggagagga actggatgag
      421 gatatcatca gtcagtattt tacggaccat ttcagcgggg actgcctgct tgcggttgga
      481 gacgaagaac tgatcaaaat tcattttcat acgaacgagc cgtggaaggt gctggaatat
      541 tgtgcttcta tcggggagat ctatgatatc gtcgtagagg atatggaacg tcagtcaaat
      601 ggtctgcacg gctgaaaaaa tacccggcgc tgccgggtat ttttttattg acatactgtg
      661 tgatacacag tataatgatg ttacacagta aataaaagaa tcaactatac aggaatcgga
      721 ggaagagaaa tgaatgaaga tgagattgtt tcgggactga ttctggaatt gcggagaggc
      781 acactgatcc tgttagtact cagccagcta aaagagccga aatacggata ttatctcgtg
      841 aaaactctgc aggagaacgg aataccgata gaagccaata cgctttatcc gcttatgcgg
      901 cggctggaga cacagggact tctttcaagt gaatgggaaa caagcgagac aaagccgagg
      961 aaatattaca ggctgacaaa agacggggag gcggtccttg aaaaggcaac gcttcattgg
     1021 aaacagttct cggcagatgt gaatcgattg ttggaggtag aaaaggatgg aaaataattt
     1081 aacagaacga tatatttatg cggtgaccag acggctgcct gcaaagatga aagaggatat
     1141 tgcaaaagag atcagaacat tgatcgaaga tatgctggag gaacgctgcg ggatcatttc
     1201 cccaacggaa aaagacgtca gagttgtatt gacagagctt ggaaccccgg acgaacttta
     1261 tcaaacctat agcggcagcg gtgaaaagtg tctgatcgga ccgccgtatt atgctcagta
     1321 tctgtttgtg ctgaagcttg tgcttttatg tacggcgttt ggcctgacag tttccctgat
     1381 cgttcagctg cttaccccaa atggagcggg gccgtggtat gagatcctgc tgcagtggtt
     1441 ctttacggta ttcagcggac tgggcatggg gtttgcctat gtgacgcttt tatttgcttt
     1501 cttttatcat aaaaaaatag agtttgatac cgcgttttct ctggatgatc t
//
LOCUS       DAOSYE010000616         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71620_length_1550_cov_2.517057, whole genome shotgun sequence.
ACCESSION   DAOSYE010000616 DAOSYE010000000
VERSION     DAOSYE010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71620_length_1550_cov_2.517057"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLU43_09860"
     CDS             <1..979
                     /locus_tag="PLU43_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOO28760.1"
                     /translation="IYKLTGGSRFQTDYSNASRTQLFNLHTLAWDSEICTWFQIPPEC
                     LPEVTDSDGDFGTTDFEGYFAHPIPIRGVLGDSHGALFGQGCHKKGMVKGTYGTGSSV
                     MMNIGDKPVLSSHGVVTSLAFCIGGKVDYVLEGNINYTGAVITWLKNDVKLIASEKET
                     EALALSANKADKTYLVPAFSGLGAPYWDSDATASVSGITRITGKAEIVRAALDCIAYQ
                     ITDIIRAMSQDAGIPIEELRADGGPTGNSYLMQFQSDIAGVNVQVSKAEELSGIGAAY
                     LAGISLGIFNKEQIFHNMRRQTYLPAMDGNERDEKYKGWKSAVSRVLTG"
     gene            1076..>1550
                     /locus_tag="PLU43_09865"
     CDS             1076..>1550
                     /locus_tag="PLU43_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO28761.1"
                     /translation="MGSTIKEISEATGFSPATVSNALNHKKGVNKETAAAIFETAHKM
                     GYVEENKVKRVKFVIFKANGLIIDDTPFFPLLIEGVQKECRNFGLEMSIYNLDKRETD
                     YESQAKALSMDASSAVILLGTELEDSDLAVFLNSKCPFLVLDYWVSDMFFNGVLIN"
BASE COUNT          438 a          299 c          425 g          388 t
ORIGIN      
        1 tatttataag ctgaccggcg gcagccggtt tcagaccgat tattcaaacg cttcccgtac
       61 acagctgttc aacctgcata cacttgcgtg ggacagtgag atctgtacgt ggtttcagat
      121 accgcctgag tgtcttccgg aagtcacgga ttccgacggt gatttcggca caacggattt
      181 tgaagggtat tttgcacatc cgataccgat caggggcgta ctcggggatt ctcacggagc
      241 actgttcgga cagggctgtc ataaaaaagg aatggtgaag ggaacatacg gaacaggctc
      301 ttctgttatg atgaatatcg gggacaagcc ggttttgagc agccatggcg ttgtaacatc
      361 gcttgccttt tgtatcggcg gaaaagtgga ttatgtattg gagggcaata tcaattatac
      421 gggagcggtc atcacgtggc tgaaaaatga cgtgaaactg atcgcatcgg aaaaagaaac
      481 agaagcactt gcgttatcag caaataaggc ggacaaaacc tatctggtcc cggcgttttc
      541 cggtctgggt gccccctact gggacagtga tgcgacggcg tctgtcagcg gcattacaag
      601 gatcaccgga aaggcggaga tcgtcagggc agcacttgac tgtattgcat atcagatcac
      661 ggatattatc cgggcaatga gtcaggatgc cggcattccg atcgaagagc tccgtgcgga
      721 cggaggtcct accggaaaca gctatctgat gcagtttcaa agcgatattg caggtgtaaa
      781 tgtgcaggtc tcaaaagcag aggagctgtc cgggatcggc gccgcttatc tggcgggtat
      841 ttcgctcggc attttcaata aggagcagat ctttcataat atgaggcggc agacgtatct
      901 gccggccatg gatggaaacg agcgtgatga aaaatataaa ggatggaaat ctgcggtaag
      961 cagagtgctg accggataaa ttgtattttg cgcaaatcat gtcccatctc ttgcttttat
     1021 ttgagtttta agagaaaata gagatgataa aaatcgaaat gagaggatgg atatagtggg
     1081 aagtacaatt aaagaaatca gcgaggcaac aggtttttca ccggcaaccg tttccaatgc
     1141 gttaaatcat aagaagggtg ttaataagga aactgcggcg gcaatttttg aaacagccca
     1201 taaaatgggc tatgtagagg aaaataaagt aaagagggta aaattcgtta tatttaaggc
     1261 caacggactg attattgacg atacaccgtt ttttccgctg ctgatcgaag gtgtacaaaa
     1321 ggaatgcagg aatttcggac tggaaatgtc catttataat ctggataaaa gagagactga
     1381 ctatgaaagc caggcaaaag cactcagcat ggatgcgtct tctgcggtga tcctgcttgg
     1441 aacggagctt gaggatagtg atcttgcggt atttttgaac agtaaatgtc cgtttctcgt
     1501 actggattac tgggtgtccg atatgttctt taacggcgtg ctgatcaata
//
LOCUS       DAOSYE010000617         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71697_length_1549_cov_2.424364, whole genome shotgun sequence.
ACCESSION   DAOSYE010000617 DAOSYE010000000
VERSION     DAOSYE010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71697_length_1549_cov_2.424364"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLU43_09870"
     CDS             <1..994
                     /locus_tag="PLU43_09870"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOO28762.1"
                     /translation="ALVEGLGGEEVEVLVHGPLGTVIKARYGLIRETKTAVMEMAAAA
                     GILLTGKEKQPLKASTFGVGEMIKDAIGRGCTNFIIGIGGSATNDGGIGMLTALGFAF
                     LDKEEKQVGIGAGALQEIASINTDKVLPALYTCRFCVACDVTNPLCGENGATYVYGPQ
                     KGVTENLKAELDQAMASYAAVTKKCLGCDFQETEGAGAAGGLGFAFLSFLHAKLQPGI
                     EIVMDAVGMDQAMEGADLVFTGEGCLDAQTAMGKAPVGVAKMAKKHGANVVALAGAVA
                     DGAEKCNENGIDAYFPIIRQSMTLDEAMKPETAKENIRKTTEQIMRLIKMSDII"
     gene            1059..>1549
                     /locus_tag="PLU43_09875"
     CDS             1059..>1549
                     /locus_tag="PLU43_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOO28763.1"
                     /translation="MRESRTWKMIIRYSRGEEVFNMVSHIAGGGLAAAITALCVIKAS
                     FSQQVLGIVGTSVYGAMLVIYYSISSIYHGLAPRIGAKKVFRSLDGCAEFLLCAGTNT
                     LFAFGVIGKASTALGWTYFGIIWGIAVTGILFNAFDPDKFKIVSVVLYLVIWWSLTAA
                     VPI"
BASE COUNT          404 a          290 c          476 g          379 t
ORIGIN      
        1 ggcgctggtg gaaggacttg gcggtgaaga ggtggaagtg cttgtgcacg gtccgcttgg
       61 aactgtgata aaggcccgct atggtttgat cagggaaacg aaaaccgcag tcatggagat
      121 ggccgcggca gcgggaatcc tgcttaccgg aaaagagaag cagccgctaa aagcgtctac
      181 gtttggtgtc ggagaaatga taaaggatgc cattggccgc ggatgtacca attttattat
      241 tggaatcggc ggcagtgcta cgaatgacgg tggtatcggc atgctgacgg cacttgggtt
      301 tgcatttctg gataaagagg agaagcaggt cggtatcgga gcaggggcac tgcaggagat
      361 tgcgtccatc aatacggaca aagttctgcc ggcgctgtac acctgccggt tttgtgttgc
      421 ctgtgatgtg acgaatcctc tgtgcggcga aaatggggct acctatgttt acggaccgca
      481 aaaaggtgta acagagaacc tgaaagcaga attggatcag gcgatggcat cttacgctgc
      541 ggtgacaaaa aaatgtctgg gatgtgattt tcaggaaaca gaaggcgccg gtgcggccgg
      601 aggacttgga ttcgcttttt tgagttttct gcatgcaaag ctgcagccgg gaatcgaaat
      661 cgtcatggat gcggtcggga tggaccaggc catggaggga gcagatctgg tatttaccgg
      721 tgaaggctgt ctggatgcac agacggcaat ggggaaagcg ccggtcgggg tggcgaaaat
      781 ggcgaaaaag catggagcaa acgtagtggc gcttgccggt gctgttgcag atggtgcgga
      841 aaaatgcaat gagaacggaa ttgatgcata ttttccgatt atccgtcaga gcatgacctt
      901 ggatgaagcc atgaaaccgg aaacggcaaa agagaatatc agaaaaacaa cggagcagat
      961 catgcgtctg ataaaaatgt cagatataat ttagcttgaa aacttttatt aaatgcaata
     1021 agatatagat gttgaagtga ttgctttgga ggaagacaat gagagagagt cgtacatgga
     1081 aaatgatcat acgttacagc cgcggcgaag aagtattcaa tatggtttcg catatcgcag
     1141 gcggcggcct ggcagcggcg ataacggcat tgtgcgtcat aaaggcttct ttctcacagc
     1201 aggtgcttgg aatcgtcggt acatccgtgt atggggcaat gctggttatc tattactcca
     1261 tatcgagtat ttatcatgga ctggcaccaa gaatcggcgc aaaaaaggta ttccggtcac
     1321 ttgacggctg tgcggagttt ttgctgtgtg ccggtaccaa tacattgttt gcgtttggcg
     1381 tgatcggaaa ggcaagtacg gcactggggt ggacgtattt cggtattatc tggggaattg
     1441 cggttacagg gatattattt aatgcgtttg accctgataa attcaagatc gtttccgttg
     1501 tgctctatct tgtgatctgg tggtcgctta cagcagcagt tccgatttt
//
LOCUS       DAOSYE010000618         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71805_length_1547_cov_3.655496, whole genome shotgun sequence.
ACCESSION   DAOSYE010000618 DAOSYE010000000
VERSION     DAOSYE010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71805_length_1547_cov_3.655496"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLU43_09880"
     CDS             complement(<1..127)
                     /locus_tag="PLU43_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOO28764.1"
                     /translation="MDGTLVKDSSNEVYPEIKEAVRLLKKKDILFIVASGRQYYSL"
     gene            complement(182..817)
                     /locus_tag="PLU43_09885"
                     /pseudo
     CDS             complement(182..817)
                     /locus_tag="PLU43_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(820..1464)
                     /locus_tag="PLU43_09890"
     CDS             complement(820..1464)
                     /locus_tag="PLU43_09890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOO28765.1"
                     /translation="MRKIILASGSPRRREILEQTGFSFEVCASQKEEIITKDDPAEAV
                     EELSRIKALDVAGQIGDRENYMIIGADTMVAVGGRNDTDDTGCSDTPDPGNAVFVHKL
                     GKPKDEADAKQMLYMLQGRTHQVYTGVTLVYDAEGDGVREHTFHAVTDVTMYPMTEEE
                     IDAYIKSGEPFDKAGAYAIQGLCARYIREIRGEYNTVVGLPIARIIKEIKEFGI"
BASE COUNT          359 a          448 c          289 g          451 t
ORIGIN      
        1 caagactgta atactgccgg ccgcttgcta cgatgaacaa aatgtctttc ttcttaagca
       61 gccttaccgc ctccttgatt tccggataga cctcattgct gctgtctttg acaagtgtac
      121 cgtccacatc cgttgctacc agacggatcg gggaaagtat gctcttccct gtgagaccag
      181 gcaattccaa cggctttgaa ataatttcgt ccgcatgatt ctcctccagc tcctgcctgt
      241 ctcgaaatcc ccagagtaca ccaaccgtat acatccctgc agatttccct gttttcatgt
      301 ccgtattcgt atcgccgaca tacatgcatt ccgacggctc caccccaaga gacgcagcaa
      361 tgaaaagcgc accctccggg cttggtttgc gtgaaaattc ctttgactgt ccctgcacga
      421 tctgaaatag atcttttcca aacagacttt ccacgacctc aacagcctgt ttatgcggtt
      481 tattggaaag tacactcaaa agaagtccct gcttctttaa ttccaaaagg acctcgggca
      541 gtccgtcata cggccgtaca tgataaccgc tgtatttccc aaatgtctcc ttatagcttg
      601 cgcgtaattc cccgaagcgt ttgtactccg tatcgccgtt ataataaagc atccgtttca
      661 caagctcatc ggccccgtcg ccgacaaatt gtttatatcg ttctgtctca taagtatgca
      721 tgccaatcag ctccagtgca agattggcgc tgtatgcgat cgattccaaa gaattcgaca
      781 gcgtaccatc caaatcaaaa ataactgctt ttatcataat taaattccaa attcctttat
      841 ctcttttatt attcttgcta tcggaagacc gacaacagta ttatattcgc cgcgtatttc
      901 cctgatatac cgtgcacaca atccctggat cgcataggcc cctgccttgt caaacggttc
      961 tccggacttt atataggcat cgatctcttc ttcggtcatc gggtacatcg taacatcagt
     1021 gactgcatga aacgtatgct ccctgacacc gtctccttct gcatcatata caagcgtaac
     1081 accggtatac acctgatgtg tcctgccctg cagcatatag agcatctgtt ttgcgtctgc
     1141 ctcatccttc ggttttccaa gcttgtgtac gaaaacggca ttcccgggat ccggtgtgtc
     1201 tgaacatccg gtgtcatccg tatcgttcct gccgccgaca gccaccatcg tatctgcccc
     1261 gatgatcata tagttttccc tgtctccaat ctgtccggca acgtccagtg ccttgatcct
     1321 tgacagttcc tcaactgctt ccgcaggatc atcctttgta atgatctctt ctttttggct
     1381 cgcacagact tcaaaagaaa aacccgtctg ttcaagtatt tccctgcgcc tcggtgaacc
     1441 ggacgccaat atgatttttc tcattcggcc ggttcctccg gaataaagat gcgttcattt
     1501 cccttttctt ttttcagcct gacgtcctgc acagcttcca tacagaa
//
LOCUS       DAOSYE010000619         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_71941_length_1545_cov_4.228188, whole genome shotgun sequence.
ACCESSION   DAOSYE010000619 DAOSYE010000000
VERSION     DAOSYE010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71941_length_1545_cov_4.228188"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="ruvB"
                     /locus_tag="PLU43_09895"
     CDS             complement(<1..628)
                     /gene="ruvB"
                     /locus_tag="PLU43_09895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOO28766.1"
                     /translation="MSKRMIETSITEEDIKIEGSLRPQYLRDYIGQEKAKQNLKVYIE
                     AAQGRNEQLDHVLFYGPPGLGKTTLAGIIANEMGVNIKTTSGPAIEKPGEMAAILNNL
                     QEGDLLFVDEIHRLNRQVEEVLYPAMEDYAIDIMIGKGASARSVRLDLPKFTLVGATT
                     RAGLLTAPLRDRFGVIHRLEFYTSEELQTIILHSARILDVEIDRKGAME"
     gene            complement(641..1249)
                     /gene="ruvA"
                     /locus_tag="PLU43_09900"
     CDS             complement(641..1249)
                     /gene="ruvA"
                     /locus_tag="PLU43_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOO28767.1"
                     /translation="MIGYLIGKVTGIYDEQIILEVNHIGYNIKMSAGTLSCLDGLGSE
                     LKVYTYLNVREDAMILYGFLTKDDLDFFKLLIQVNGIGPKGALAILSVMTTDDLRFAI
                     LSADAKTIAKAPGIGAKTAQRLIIDLKDKMSYEDAFEQKYAHAKPGSENGMSDMCSEA
                     AEALSALGYASSDALRAVRQVELSSEDTVETVLKKALKKMII"
     gene            complement(1267..>1545)
                     /locus_tag="PLU43_09905"
     CDS             complement(1267..>1545)
                     /locus_tag="PLU43_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOO28768.1"
                     /translation="PYSMIYGMAGGILSLIFMCFFKRCKNFSIVGVSIAGGIFHNIGQ
                     LAVAAFVVKELRIAYYAPLLIIAGTIAGFLIGIVSGILYKRLSGLKNF"
BASE COUNT          399 a          383 c          303 g          460 t
ORIGIN      
        1 gctccattgc accttttctg tctatttcca catcgagtat tctggcagaa tgtaaaatga
       61 tcgtctgcag ttcctcactt gtataaaatt ccagtctgtg gataacacca aaacgatctc
      121 ggagcggtgc tgtcagcagc ccggctcttg ttgtcgctcc aacaagtgtg aatttgggaa
      181 ggtcaagacg gactgaacgg gcggacgctc cttttccgat catgatgtcg attgcgtaat
      241 cttccattgc aggatacaaa acttcctcca cctgtcggtt caaccggtga atttcgtcaa
      301 caaataacag atcgccttcc tgcagattat tgagaatggc tgccatttcc cccggctttt
      361 caattgccgg tcctgatgtc gttttgatat tgacacccat ttcatttgca atgataccgg
      421 caagcgttgt tttcccaagg cccggaggac cataaaacaa tacatggtca agctgctcgt
      481 ttctcccttg agccgcttct atataaactt taagattctg ctttgctttc tcctgcccga
      541 tgtaatcgcg cagatactgc ggccggagac tgccttcgat tttgatgtct tcttctgtga
      601 ttgatgtttc tatcattctt tttgacatga aacttcctct ttatatgatc attttcttca
      661 gcgctttttt caaaacagtt tctactgtat cttcactgga aagctcgacc tgtctgactg
      721 cacgcagagc atccgacgat gcatagccaa gcgcagaaag tgcttccgct gcctcgctgc
      781 acatatcact catcccgttt tcgctgcctg gctttgcatg tgcgtacttt tgttcaaagg
      841 catcctcata agacatttta tccttcagat caataatcag acgctgtgcg gttttggccc
      901 cgatacccgg tgcttttgca attgttttgg catctgccga gagaatcgca aaacgcaggt
      961 cgtccgttgt cataacggat aggatcgcaa gcgctccttt ggggccgatt ccattgacct
     1021 gaataaggag tttgaaaaaa tcaaggtcat cttttgtcag gaagccatat aagatcattg
     1081 catcttccct gacattcaga taagtatata ccttaagttc gcttccaaga ccgtccagac
     1141 agctaagcgt accggcagac atcttgatat tataaccgat atggttgact tccagtatga
     1201 tctgttcatc atagattccc gtaacttttc cgattaaata gccgatcatt ttagtttcct
     1261 ctcatatcag aagtttttca gaccagatag tcttttatat aaaataccgg aaacaatacc
     1321 gatcaaaaaa ccggcaattg ttccggcaat gataagcagc ggcgcataat acgcgatccg
     1381 gagttctttt acgacaaatg ccgcaacggc cagctgtccg atattatgaa aaatacctcc
     1441 ggcaatactg acaccgacga tactgaaatt tttacatctt ttgaaaaaac acatgaaaat
     1501 gagactcaaa ataccgccgg ccataccata tatcatacta tacgg
//
LOCUS       DAOSYE010000620         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72134_length_1543_cov_3.518817, whole genome shotgun sequence.
ACCESSION   DAOSYE010000620 DAOSYE010000000
VERSION     DAOSYE010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72134_length_1543_cov_3.518817"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="PLU43_09910"
     CDS             <1..1503
                     /locus_tag="PLU43_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO28769.1"
                     /translation="ARLTKLGIPVDSAVGNEALQLIGRLQFSQNHAIVIDDYQQLSSP
                     EINDFFEMLARSEMENLHIILNTRFSGFQKHEELALKGLLYHIRQEAFELRPEEIREY
                     YKICGIPPSKEQVRQLFSMTEGWISALYLMMLAYLSNGTLETPGNIYQLIEKAVHLPL
                     SDEAKDFILCVSVFDSFTLQQAAHLWKQSDSGRLLSEILRQNAFVNYDSKSKTYRIHS
                     LYREYLQDLLNKKEAAYRYALYQRAADWYQKNSDYITARHFWHLCKDYENLLQSIENG
                     KARNFTKQNLKKLQMYFDSCPETIRARHPYALLIFAVHLFVHNERNGFLMIYREANQT
                     ILSDPALETKQRNELLGELEILNGFSVFNDLKKMAQSFEKAWQLLGKTTSIYDIESDW
                     TFGSPSILYLYYRESGKLKEHLADLMTGLPCYCRLTNGNGRGGEYVMEAEWYYHKGDL
                     INAEISINKAIHKARASSQWSIALTIMFLQMRIAFMKGDYKNLSYLPGFS"
BASE COUNT          475 a          342 c          323 g          403 t
ORIGIN      
        1 gcacggctga ctaaacttgg cattcccgtt gattcggctg tcggaaatga agcactgcag
       61 ctcataggca gattgcagtt ctctcaaaac catgccattg tcattgacga ctatcagcag
      121 cttagcagcc ctgaaatcaa cgactttttc gaaatgcttg ccagaagcga aatggaaaat
      181 cttcatatca ttctcaatac gcgtttttcc gggttccaaa agcatgagga actggcatta
      241 aaaggacttc tttaccatat cagacaggag gcatttgaac ttcgtccgga agaaatcagg
      301 gaatattata aaatctgcgg aataccacca agtaaagagc aggtgcggca gcttttttca
      361 atgaccgaag gatggatcag tgccctctat cttatgatgc ttgcctacct ctcaaacgga
      421 actttagaaa ctcccggcaa tatctatcaa ttgatcgaaa aggccgtaca tctgcctctt
      481 tcggatgagg caaaggattt tattctgtgt gtatccgtat ttgacagttt tactttacag
      541 caggctgcac atctatggaa acagtccgat tcaggccggc tgctttccga aattcttcgc
      601 caaaatgcat ttgtaaatta tgacagtaag tcaaaaacct atcggatcca cagcttatac
      661 agagaatacc tgcaggatct gctgaacaaa aaagaagccg cctaccggta tgctctatat
      721 caaagagcag cggactggta tcaaaaaaac tccgattaca ttacagccag acacttctgg
      781 cacttatgta aagattatga aaatctgctg cagtcgatcg aaaacggaaa agccagaaat
      841 ttcacaaaac agaacctgaa aaaactgcag atgtattttg actcctgccc ggaaaccatc
      901 agagccagac atccttatgc tcttctaatt tttgccgtcc atttatttgt tcataatgag
      961 aggaacgggt ttctcatgat atatagggaa gcaaaccaaa ctattttatc tgatcccgct
     1021 cttgagacaa aacaacgaaa cgaattgctt ggagaactcg aaatactcaa cggattttct
     1081 gtcttcaatg atctgaagaa aatggcacaa agctttgaaa aagcatggca gctgttagga
     1141 aagaccacct cgatctatga cattgaatcg gactggacct tcggctctcc ttctatcctg
     1201 tacctttatt acagggaaag cggaaagctt aaagagcatt tggcagactt aatgacagga
     1261 ctgccgtgct attgccggct cactaacggc aacggccgcg gcggcgaata tgtgatggaa
     1321 gcagaatggt attatcataa gggagatctc ataaacgctg aaatatccat taacaaggcg
     1381 attcataaag cacgtgcatc cagccagtgg agcattgcgc tgaccatcat gtttttacag
     1441 atgcgcatag cctttatgaa aggcgactat aaaaatttgt cttatctgcc tggattctcg
     1501 tgactcgtga cgagaacatc gtttattgag tgacttcaaa ctg
//
LOCUS       DAOSYE010000621         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72139_length_1543_cov_3.329973, whole genome shotgun sequence.
ACCESSION   DAOSYE010000621 DAOSYE010000000
VERSION     DAOSYE010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72139_length_1543_cov_3.329973"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1543
                     /gene="cysS"
                     /locus_tag="PLU43_09915"
     CDS             133..>1543
                     /gene="cysS"
                     /locus_tag="PLU43_09915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO28770.1"
                     /translation="MKIYNTLSGKKEEFIPLQPGKVRMYVCGPTVYNLIHIGNARPMI
                     VFDTVRRYMEHKGYEVNYVSNFTDVDDKIIKAAIEEGVEASVISERYIEECKKDMAAM
                     NVKPATTHPKATEEICGMLDMISILIEKGYAYAAGDGTVYFRTRRFQSYGKLSHKNLD
                     DLQGGNRSLLISGEDQKEDPLDFVLWKPKKEGEPYWESSWCKGRPGWHIECSVMSKKY
                     LGDEIDIHAGGEDLIFPHHENEIAQSEAANDKPFAKYWMHNAFLNIDNKKMSKSSGNF
                     FTVRDISQKYDLQILRFFMLSAHYRSPLNFSAELMEASQNSLERILTAVENLKYLISN
                     NTQDNAPCSEAETALLKEAAAFKEKFDEAMDDDFNTADAISVIFELVRFINTNTDVQN
                     TKEYLQALLEELIGLADILGLLVDKKEEILDEEIEALIAKRQTARKEKNFARADEIRD
                     ILLSQGIVLEDTREGVKWHK"
BASE COUNT          475 a          294 c          416 g          358 t
ORIGIN      
        1 ggatcagatc cacttgcctg atccggtccg cgaggacatt cagaatctgc agaaggcaaa
       61 cagtgagctg acgaaccggc ttctggatat ggagcaggaa atcaaagatt tgaaaaagga
      121 gaaacaggtc atatgaaaat ttataatact ctgtcgggta agaaggaaga atttatacca
      181 ttacagccgg gaaaggtcag aatgtatgtg tgcggaccga ctgtttataa tctgatccat
      241 atcggcaatg cacgtcctat gatcgtattt gatacggtca ggcgctatat ggagcataag
      301 ggatatgaag tgaactatgt atccaatttt acggatgtag atgataagat cataaaagca
      361 gccattgaag agggcgtgga agcgtccgtg atctcggagc ggtatattga ggaatgtaaa
      421 aaagatatgg cagctatgaa tgtaaagcct gcaacgacac atccgaaggc aacggaggaa
      481 atatgcggca tgctggatat gatcagcatt ctgatcgaaa aagggtatgc ctacgcggca
      541 ggagacggca cggtctattt cagaacgcgc agatttcagt cctacggaaa gctgtcccat
      601 aaaaacctgg atgatctgca gggtggtaac cgttccctgt tgatatcagg tgaagaccag
      661 aaagaggatc cgcttgattt cgttctctgg aagccgaaaa aagaaggaga gccttactgg
      721 gaatcgtcct ggtgtaaagg aaggcccggc tggcatatcg aatgctccgt aatgagcaag
      781 aaatacctgg gggatgagat cgatattcat gcgggcgggg aggatctgat ctttccgcat
      841 catgagaacg agatcgcgca gagcgaagct gcaaacgaca agccgtttgc gaaatactgg
      901 atgcacaatg cgtttttgaa cattgacaac aaaaagatga gcaaatcgtc cggaaatttc
      961 tttacggtcc gtgatatcag tcagaaatat gatctgcaga tcctgcgttt tttcatgctg
     1021 agcgcacatt accgcagtcc gcttaatttc agtgccgagc ttatggaagc ctcgcagaat
     1081 tctctggaga ggatcctgac ggcagtggaa aatctgaagt atctgatcag caacaatacg
     1141 caggataatg ccccatgttc ggaagcggag actgctttat taaaagaggc ggctgctttt
     1201 aaagaaaagt tcgacgaggc aatggatgat gattttaata cggccgacgc aatctccgtt
     1261 atatttgagc ttgtcaggtt tattaacaca aatacggatg tacagaatac aaaggaatat
     1321 ctgcaggcgc ttcttgagga gctgatcggt cttgcggata tccttggtct gctcgtagac
     1381 aaaaaggaag agatcctgga tgaagagatc gaggccctga ttgcaaaacg gcagacggca
     1441 agaaaagaaa aaaactttgc acgtgctgat gagatccgtg atattctgct ttcgcaggga
     1501 atcgtgctgg aagacactag agaaggtgta aaatggcata aag
//
LOCUS       DAOSYE010000622         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72185_length_1543_cov_2.062500, whole genome shotgun sequence.
ACCESSION   DAOSYE010000622 DAOSYE010000000
VERSION     DAOSYE010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72185_length_1543_cov_2.062500"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLU43_09920"
     CDS             complement(<1..898)
                     /locus_tag="PLU43_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOO28771.1"
                     /translation="MKKIVLLVICFGIMLPGLTGCKEEAPEKNQITNEVQEEEPEETE
                     EEEVIVKEGTLERVEVDSAAIAENKIEENTEQKLNIYLPPSYQESDKSYPVVYFLHGF
                     GDSPSVFVRNAKKVLDVEFSKDPSKEFILAAVDGSNSAGGSFYVNSPITGNWEDYTVT
                     EVVSYIDQNYRTIPKAEARGICGFSMGGFAALNLAFRHPDVYTAVYAMSPGVLAPGKI
                     EEALATWEGDQVFLKAYSMAFAYAEEPPYVAIPAEDGSDADQLLLERWESGFGNWQEK
                     LDEYLSMQTPLKAIGFCYGTNDG"
     gene            1036..>1543
                     /locus_tag="PLU43_09925"
     CDS             1036..>1543
                     /locus_tag="PLU43_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO28772.1"
                     /translation="MNTINHKLIENAVIFIENNLKNNLSLDELSMQLCISKYHLHRIF
                     RSITGIPLMTYVRKRRLSDSLTALRDEKLKIIDIACEYQFEYVQSYERAFRQLFGITP
                     LFFRKNHCELQIYPRFDTSLLQNTADGIVIPPFYVTKPKFYLAGIKTLIYHQENYASG
                     TANSNALNF"
BASE COUNT          436 a          376 c          274 g          457 t
ORIGIN      
        1 atccgtcatt tgttccatag caaaaaccga tcgcctttag cggtgtctgc atggaaaggt
       61 attcatccag cttttcctgc cagtttccga acccggattc ccagcgttcc aacagcagct
      121 gatcggcatc tgaaccgtct tctgcaggaa tggcaacata cggcggttct tcagcatagg
      181 caaaggccat ggaataagcc tttaaaaaaa cctggtctcc ttcccatgta gcaagggcct
      241 cctcaatttt gccgggcgcc aaaactccgg gagacatcgc ataaactgca gtatacacat
      301 ccggatgcct gaaagccaga ttcagagctg caaatcctcc catggaaaat ccgcagattc
      361 cccttgcctc tgctttcgga atcgtcctgt agttctggtc aatataggaa acaacttccg
      421 tcaccgtata gtcttcccaa ttcccggtta tcggggaatt cacataaaaa gaaccgccgg
      481 ctgaatttga accgtcaaca gccgcaagaa taaattcttt cgacggatcc ttcgaaaatt
      541 cgacatccag tacctttttg gcattacgta caaagacaga aggggaatcc ccaaacccat
      601 gcagaaaata aacgaccgga taagatttat cggattcctg ataagagggc ggaagataga
      661 tattcagctt ctgttccgtg ttttcttcta ttttgttctc ggcaatagct gcggaatcta
      721 cttctacacg ttccagtgtt ccttctttta caatgacttc ctcttcttct gtctcttccg
      781 gttcctcttc ctgcacctca ttcgttatct gatttttttc cggtgcttct tctttgcatc
      841 ctgtcagtcc aggcaacatt attccaaaac aaataaccag taaaactatt tttttcataa
      901 gtccctccgc ttaaagtttt tttgacatca ttttatgaat agtatatgat gatctcataa
      961 aataaagtga acatttcttg ctgatttttt cccttattga caataccgct gctctgctgc
     1021 gaaacggaga ttcttatgaa tacgatcaat cataagctga ttgaaaatgc tgttatcttt
     1081 attgaaaata atttaaaaaa taatctgtcc cttgatgaac tttccatgca gctttgcatt
     1141 tcaaaatatc acctgcacag aattttcaga tccattaccg gcattccact gatgacttat
     1201 gtccgcaaaa gaagattatc cgacagtctt accgcacttc gtgatgaaaa attaaaaatc
     1261 atcgacattg cctgtgaata tcaatttgaa tatgtccaga gctatgaacg tgcattcagg
     1321 cagctgttcg gaattacacc tctgttcttc cggaaaaatc actgcgaact ccaaatttat
     1381 ccgcggttcg atacaagcct gctgcaaaat acagcagatg gtattgtgat cccgcccttt
     1441 tatgtgacaa aacccaaatt ctatctggct ggaatcaaaa ccttgatata ccatcaggaa
     1501 aactatgcgt caggaactgc aaattcaaat gcactgaatt ttt
//
LOCUS       DAOSYE010000623         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72308_length_1541_cov_2.970390, whole genome shotgun sequence.
ACCESSION   DAOSYE010000623 DAOSYE010000000
VERSION     DAOSYE010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72308_length_1541_cov_2.970390"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1203
                     /locus_tag="PLU43_09930"
     CDS             154..1203
                     /locus_tag="PLU43_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28773.1"
                     /translation="MDYKKKKRNVKSVQDMMGIGMDVERSKHKSNYQGPYKKINEPDR
                     PSGGSYRQSVPSTGMKSAYKPMTRTGGFQDFAWIIFSSILVVCIIITCIVYSRQAKAS
                     NYTYPLDVVTMTGVLNTIYEADTQTAASADSTQTDSAADTGQTDAASTDGTQTDGTQT
                     DAAAADSTQTATSGASMVLDSGEGMTGYTEAASYTELLSQLEGALAAEDSAFVGSKLS
                     YKDETSGALTGYPQSIVEYFTSYMAANSDKRTLFMESIQDEATYAGQNGTAYVVVLPV
                     IQYTVKTSYDNTTFSFSGFSEQTINTNQTATVSPMLPCMYTVTASNAEWEQVLTSEME
                     AKFGENLELNFGVNE"
     gene            1232..>1541
                     /locus_tag="PLU43_09935"
     CDS             1232..>1541
                     /locus_tag="PLU43_09935"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28774.1"
                     /translation="MKLQIDTLKLSVIVPCYNEKDTIEGVIASIRDCGIKNLEIIIVE
                     DCSTDGTREFLKEKVGATVEKVLYHKVNRGKGAALRNGFKAATGDLVVIQDADMEYDP
                     R"
BASE COUNT          475 a          292 c          419 g          355 t
ORIGIN      
        1 ctagcttcaa taaatactat agagcggaca ttgcgatctg tcaagaaaaa ataaggtcta
       61 cacaaatttt aaatttatgg tagaatagaa atgatgcata gataaagaat ttttttatca
      121 tgtatgcagc gggaaaggaa atatggaaca aaaatggatt ataaaaagaa aaaaagaaat
      181 gtaaagtcag tacaggacat gatggggatc ggcatggatg ttgaaaggtc gaagcataaa
      241 agcaactatc agggacctta taaaaaaata aatgagccgg accgcccttc aggcggaagc
      301 tacagacaga gcgttccgtc tacgggcatg aaaagcgcgt ataagccaat gacgcgtacg
      361 ggtggttttc aggattttgc atggatcatt ttcagctcga tcctggtcgt gtgtattatt
      421 attacatgca ttgtctattc cagacaggca aaggcttcaa attatacata tcctttggat
      481 gtggtgacga tgacaggggt gctgaatacg atctatgaag cagatacgca gacggcggca
      541 tcggcagata gtacacagac agattcagcc gcggataccg ggcagacaga tgctgcatcg
      601 acggacggta cgcagacgga tggtacacaa acagatgctg cagcggcaga cagtacgcag
      661 acggcaactt ccggagcgtc catggtgctt gacagcggag agggcatgac aggatatacg
      721 gaagctgcat cctatacgga actgttaagt cagctggaag gcgcacttgc agctgaggac
      781 agtgcgtttg tcggcagcaa gctttcctat aaggatgaga cgagcggggc actgacaggt
      841 tatccgcagt cgatagtaga gtattttact tcctatatgg cagcaaattc tgataaacgg
      901 acgttattta tggagagcat tcaggatgag gcgacctatg caggacagaa cgggacggct
      961 tatgtggtcg tgttgccggt gatccaatat actgtaaaaa caagctatga taatacgaca
     1021 ttttcttttt ccggattttc cgaacagacg atcaatacga atcagaccgc aacggtcagt
     1081 ccgatgcttc cgtgcatgta tacggtcact gcgtccaatg ccgaatggga gcaggtgctc
     1141 acttctgaaa tggaagcaaa attcggagaa aatctggagt tgaattttgg tgtgaatgaa
     1201 taaaacaaaa gcatgagagg agatattgcc gatgaagctt caaatcgata cgttaaagct
     1261 gtcggtcatt gtgccgtgtt acaatgaaaa ggatacgatc gagggtgtga tcgcgtcgat
     1321 ccgcgactgc ggaatcaaaa atctggagat catcattgtg gaggactgtt ccacagacgg
     1381 tacccgtgag tttttaaagg aaaaggttgg cgccaccgtg gaaaaggtgc tttaccataa
     1441 ggtaaaccgc ggaaagggtg cggccctgcg caatggattc aaagcggcga ccggagatct
     1501 tgtagtcata caggatgccg atatggaata tgatcccagg g
//
LOCUS       DAOSYE010000624         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72408_length_1540_cov_2.661279, whole genome shotgun sequence.
ACCESSION   DAOSYE010000624 DAOSYE010000000
VERSION     DAOSYE010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72408_length_1540_cov_2.661279"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..811)
                     /locus_tag="PLU43_09940"
     CDS             complement(47..811)
                     /locus_tag="PLU43_09940"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOO28775.1"
                     /translation="MKLLQACIIAFSTYSKIPMPKVDWNKENKKYAICFFPMVGVLIG
                     AVCIVWYIICSRYQITGVVRANMPALFILLLTGGIHTDGYMDTMDALHSYAETEKKLQ
                     ILKDPHIGAFAVISVIGYYLAYTSFVSMIGRWDLSLLYALGFVLSRILSGLGVVYLKN
                     AKQEGMLYEFSDAAHKRTVRIVLFFLLGICAALMLSVSFAAAVCVLAANGFLYCFYRY
                     KAYREFGGITGDLAGWFLVLSELISAGIPALFSIRF"
     gene            complement(842..1345)
                     /locus_tag="PLU43_09945"
     CDS             complement(842..1345)
                     /locus_tag="PLU43_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOO28776.1"
                     /translation="MILITGGSGSGKSAFAETLAQEYHKTGVLYYMAAMKCDTKEGRQ
                     RVLRHKRQRAGKGFITVEQPYDVWMLQAGHDSTILLECLSNLLANEWFDRHRSARQIL
                     QEIRFLEESCENLLIVTNEIFSDGIGYDEMTKDYIRQLGWLNHELAGIADTVYEVVYG
                     IPVRIKG"
     gene            complement(1345..>1540)
                     /locus_tag="PLU43_09950"
     CDS             complement(1345..>1540)
                     /locus_tag="PLU43_09950"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOO28777.1"
                     /translation="KEPAMRLILEELGKEPVLKAQLALGEGTGGVMLLPLLDMAVSIY
                     QENATFQELSIKEYKEYETR"
BASE COUNT          431 a          420 c          279 g          410 t
ORIGIN      
        1 ctccgataaa tagttccata ttcttcctcc cacctttttc tccatatcag aaccgtatgg
       61 aaaagagcgc aggaatccct gcactgatca attccgacaa aaccaggaac cagccggcca
      121 gatcacctgt gatcccgccg aattccctgt atgccttata gcggtaaaaa cagtataaaa
      181 agccattagc cgcaagcaca catacggcag cggcaaaaga caccgacagc atcaatgcgg
      241 cacagattcc cagtaaaaaa aacaaaacga tccgcaccgt acgtttatgg gcagcatccg
      301 aaaattcata aagcatgcct tcctgctttg cgttttttaa atatacgacc cccagcccgc
      361 ttaagatccg ggataaaaca aaacccaatg cataaagcag acttaaatcc cacctgccga
      421 tcatggaaac aaacgacgta taggccagat aatacccgat cacactgatc acggcaaacg
      481 caccgatatg agggtccttt aatatctgca gttttttttc tgtctctgca taggagtgca
      541 gggcatccat cgtatccatg taaccgtccg tatgaattcc ccccgtaagc agcagaataa
      601 acaatgccgg catatttgcc cggacgaccc cggttatctg atatctgctg caaattatgt
      661 accagacaat gcagacagct ccgatcagta caccgaccat cgggaaaaaa cagatcgcgt
      721 attttttgtt ttccttattc cagtccactt ttggcatcgg tattttagaa tatgtagaaa
      781 acgcaatgat acatgcctgc aaaagtttca tatttgcacc aatccctttc tgattaacag
      841 atcagccctt gatccgcacg ggtatgccat agacgacttc atatacggta tccgcaatac
      901 ctgccaattc atgattcagc catccaagct gccggatata gtctttcgtc atttcatcat
      961 aaccgatccc gtctgaaaaa atttcatttg ttacgatcag cagattttca cagctttcct
     1021 ctaaaaacct gatctcctgc aggatctgtc ttgcagatct gtgcctgtcg aaccattcat
     1081 ttgcgagcag attagaaaga cattccagca aaatcgtact gtcgtgtcct gcctgcagca
     1141 tccacacatc ataaggctgt tcgaccgtaa taaatccttt cccggcacgc tgccttttat
     1201 gacgcagaac acgctgcctg ccttcttttg tatcgcattt cattgcggcc atataataaa
     1261 gaacacctgt tttatgatat tcctgtgcaa gtgtttcagc aaatgccgat tttccgctgc
     1321 cgctgccccc tgtaataaga atcatcacct tgtttcatac tccttgtatt ctttgattga
     1381 aagttcctga aacgttgcat tttcctggta tatggaaacg gccatatcaa gaagcggcag
     1441 cagcataaca ccgcctgttc cttctccaag tgccagctgt gctttcagaa ccggttcctt
     1501 tccaagttcc tctaagatca aacgcattgc cggctccttt
//
LOCUS       DAOSYE010000625         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72480_length_1539_cov_2.933288, whole genome shotgun sequence.
ACCESSION   DAOSYE010000625 DAOSYE010000000
VERSION     DAOSYE010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72480_length_1539_cov_2.933288"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLU43_09955"
     CDS             complement(<1..320)
                     /locus_tag="PLU43_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28778.1"
                     /translation="MEENVRLIERTFHILEVLSLSQQPMSLSEIVRESGMSKTTVYRL
                     LTTMNEMGYIQKNNELRYSIGYKFMELASYHINHLELQTEAKPFLTSLYSELNLTVHL
                     GTLEG"
     gene            531..>1539
                     /locus_tag="PLU43_09960"
     CDS             531..>1539
                     /locus_tag="PLU43_09960"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOO28779.1"
                     /translation="MLQASVGTSKLTNGFEAGLEAAKAAAKELEQVSIIFAYFSCAYD
                     TAQVLKGIKEVFADAPVIGNTSFTGIITKEGYVGGDEPFVGIMGMYADGLTVGTAMVK
                     GNGKPYEEGVDLAKAAMAAAGKTSAPDYFYMAASSGEEEFYLKGISSIVGRIPFFGGS
                     AADNSIAGEWKLYAGTEFCESGAAVAFFYDAPMMKNVFTGAYHETNDFGVITKIDGNR
                     TLMEIDGVPALEKYAQWRGCSIDDIKGGNLLVQTVCSPLGVKDRLGDLIAIRHPMNGN
                     DDLSMAIGNNLAEKTCIIRMEATVDELIEAVPKTLAALNERIPGKPLAYHLVHCGGRR
                     AG"
BASE COUNT          456 a          302 c          375 g          406 t
ORIGIN      
        1 ccttccagcg ttccaagatg cacggtcaga ttcaattcgg aataaagtga agtcaaaaac
       61 ggcttcgcct ctgtctgaag ctccagatga ttaatatggt agctggcaag ctccataaat
      121 ttatatccaa tagaataacg gagctcatta tttttctgaa tataccccat ttcattcatc
      181 gtagttaaaa gacgatatac tgttgtcttg ctcatccctg actcacgaac gatctcactt
      241 agcgacatcg gctgctgtga gagagaaagt acctccaaaa tgtgaaatgt gcgttcgatc
      301 agccgaacat tttcttccaa gtattatcag ctcctttctt ccttatacgc gacttatctc
      361 ttctattatg gaacaaatca taaagaaaat caatcaaatt gcgtaattgt tccgctgctc
      421 ggaacagctg tttctgtgag cggaattttg tggtttttat tttgttttgt tgatttatac
      481 taaaaataac aatgtattcg ggaatttatt cagcaaaagg aggaataaat atgttacagg
      541 caagtgttgg gaccagcaaa ttgactaatg gttttgaagc aggactggag gcagccaagg
      601 cagccgcaaa agaattggaa caggtaagta ttatttttgc ttatttcagc tgtgcgtatg
      661 atacggcaca ggttttaaag ggaatcaaag aggtcttcgc cgatgcaccg gtaatcggaa
      721 atacttcttt caccggtatc attacaaaag aaggatatgt cggaggtgac gaaccattcg
      781 tcggtatcat gggtatgtat gcggacggtc ttacggtcgg gaccgcaatg gtaaaaggaa
      841 acggaaaacc ttacgaagaa ggcgttgatc tggcaaaggc tgctatggca gcagcaggaa
      901 aaacgtctgc acctgattat ttttatatgg cagcatcatc cggtgaagaa gaattttatc
      961 tgaaagggat cagcagcatc gtcggaagaa ttcctttctt tggcggaagt gcggcagata
     1021 acagcattgc aggtgaatgg aagctttatg ccggaacaga attttgcgaa agcggtgcgg
     1081 cagtcgcatt tttctatgat gcgccaatga tgaaaaatgt atttacagga gcttatcacg
     1141 aaaccaatga tttcggtgtg atcactaaaa tagacggaaa ccggacactg atggagatcg
     1201 acggcgtacc ggctcttgaa aagtatgcac agtggagggg ctgcagtatc gacgacatca
     1261 aaggcggaaa tcttctcgtt cagacagttt gctctccgct cggtgtaaaa gaccggctgg
     1321 gagatctgat cgcgatcaga catccgatga acggcaatga cgatctgtcc atggctattg
     1381 gaaataatct tgcggaaaag acctgtatca ttcgcatgga agcaaccgta gatgaactga
     1441 tcgaggcggt tccgaaaaca cttgcagcat taaatgaaag gattccggga aaaccgctgg
     1501 cttatcatct tgtacactgc ggcggaagga gagccggaa
//
LOCUS       DAOSYE010000626         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72579_length_1538_cov_2.881322, whole genome shotgun sequence.
ACCESSION   DAOSYE010000626 DAOSYE010000000
VERSION     DAOSYE010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72579_length_1538_cov_2.881322"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLU43_09965"
     CDS             complement(<1..656)
                     /locus_tag="PLU43_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO28780.1"
                     /translation="MDFALSKEHEMARQLFKDFAEKEVKPLAQEVDETERFPSETVEK
                     MAKYGFLGIPVPKEYGGQGADILTYAMCVEELSKVCGTTGVVVSAHTSLCIDPILTYG
                     TEEQKQKYVIPLAKGEKLGAFGLTEPGAGTDAQGQQTKAVLDGDEWVLNGSKCFITNG
                     KEADVYIVIAVTGKIEKRGKKQKEISAFIVEKGTPGFTFGTKENKMGIMPINKLLLTM
                     S"
     gene            complement(758..1537)
                     /locus_tag="PLU43_09970"
     CDS             complement(758..1537)
                     /locus_tag="PLU43_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOO28781.1"
                     /translation="MTDGYEVCLCDIKTEYAEGGKAKIQKNMDFLVGKGKIEKEKADA
                     IMGKITTGLNDICTDCDLIIEVALEKLEIKQSIFKELMGIVKKDCMFASNTSSLSITK
                     IGDGLDRPMIGMHFFNPADRMKLVEVIKGQNTPQDMVEKIKSIAVEIGKTPVEVKEAP
                     GFVVNRILVPMINEAIGVFDAGTASAEDIDVAMQLGASHPMGPLHLGDLIGLDICLVI
                     MEVLYTETKDEKYAPQPLLKKMVEEKKLGRKTGIGFFDYSK"
BASE COUNT          373 a          403 c          271 g          491 t
ORIGIN      
        1 gacatggtca acaacagttt gttgattggc attatcccca tcttgttttc ttttgtaccg
       61 aatgtaaatc cgggtgtccc tttttcaacg ataaacgcag aaatttcctt ctgctttttt
      121 cctctcttct cgatcttgcc cgtaactgcg atcacaatat aaacgtctgc ttctttaccg
      181 tttgtaataa agcattttga accgtttaat acccactcgt caccgtctaa tacagccttt
      241 gtctgctggc cctgtgcatc cgtgccggca cccggctctg taagaccaaa ggcaccgagc
      301 ttttcgccct ttgcaagcgg aattacatat ttctgcttct gttcttccgt accatacgta
      361 agaatcggat caatacaaag agaagtgtgc gcagaaacaa ccacgcctgt cgtaccgcag
      421 acttttgaaa gttcttccac acacattgcg taagttaaaa tatcggctcc ctgtccgccg
      481 tactctttcg gaaccggaat tccaaggaat ccatattttg ccatcttttc aactgtctca
      541 ctcggaaagc gttctgtttc atcgacttcc tgcgcaagag gttttacttc tttttcggcg
      601 aaatccttaa acagctgtct cgccatttca tgttctttac ttaatgcaaa atccatgatc
      661 ttcattcctc catatttata ttgtagttat tttgttagac gcccatgacc gtgtcggctg
      721 ccgaccggca catgagcgcc attgcattta aattccatta ttttgaataa tcaaagaaac
      781 caataccggt ctttctgcca agcttctttt cttcgaccat tttcttaaga agcggctgcg
      841 gtgcgtattt ttcgtctttt gtctccgtat agagaacttc catgatcaca agacagatat
      901 ccagtccgat cagatctccc aaatgaagag gtcccatcgg gtgggaagcg ccaagctgca
      961 tggcaacatc gatatcttcc gcggatgccg tacctgcatc aaaaacaccg atcgcttcgt
     1021 tgatcatcgg aacgaggatc ctgtttacaa caaaaccggg agcttccttg acttctaccg
     1081 gtgtctttcc gatttccaca gcaatgctct tgatcttttc aaccatatcc tgcggtgtat
     1141 tctgaccttt gattacttcc acaagcttca ttctgtcggc cggattaaag aaatgcatac
     1201 cgatcatcgg ccggtcaagt ccgtctccga tttttgtgat ggaaagagac gacgtgttcg
     1261 aagcaaacat acaatccttt tttacaattc ccattaactc tttgaaaata ctctgcttga
     1321 tttccagttt ctcaagcgca acctcgatga tcagatcaca gtctgtacaa atatcattga
     1381 gaccggtcgt gatctttccc atgatcgcgt ctgctttttc tttttcaatt tttcccttac
     1441 caacaagaaa atccatgttt ttctggatct tagcctttcc gccttcagca tactccgttt
     1501 taatgtcgca aagacatact tcataaccat ctgtcatt
//
LOCUS       DAOSYE010000627         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72602_length_1538_cov_2.310856, whole genome shotgun sequence.
ACCESSION   DAOSYE010000627 DAOSYE010000000
VERSION     DAOSYE010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72602_length_1538_cov_2.310856"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLU43_09975"
     CDS             <1..935
                     /locus_tag="PLU43_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO28782.1"
                     /translation="SRAMSGKGRIGEETRQRVNTYIAKHGYQPNVIAKGLAQSRTFNI
                     CVLLPEDDYLKDMPFFQKVLLGISEYLSGADYDAIVATASEHNIENLKRIVTNKKVDG
                     VVLTRTVKNDPAVLFLQKQKIPFVVTGLYEGADVVQVDARHEEAACELTRYLLGKGLK
                     RISLLGGNSGHIVSQKRLLGVEQAYEIMRIDPKYLSVYENMNSESDITRAMQTILYES
                     CDCILCADDRITISALNKLRKANVKIPEEIRVATLFESVILNNYVPAITGLTYDVMQL
                     GRIVAEQILLLIQGKQVEKLTTLDYQIVIKESTR"
     gene            959..>1538
                     /locus_tag="PLU43_09980"
     CDS             959..>1538
                     /locus_tag="PLU43_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28783.1"
                     /translation="MVKKKKTSMEYRYYDMPPDEPVLALLGDGWKRKYGEGIKFLHFH
                     NILEIGYCHYGRGELTYDVMEDELLCRKYAGGEVSVIPRSIPHTTNSAPGSVCYWEYL
                     FVDVTNFLGEYTEKNPIQAEKVIERINARPLLIKEEEHTLLTDAVKGILRVQKEKQPY
                     YMEITNGLVFTMLMLIAQANRAYSKQNEESSSV"
BASE COUNT          475 a          276 c          419 g          368 t
ORIGIN      
        1 tttccagggc aatgtccgga aaaggcagga tcggagagga aacaagacaa agagtgaaca
       61 catatattgc caaacatgga tatcagccga acgtgatcgc caagggactg gcacagtcca
      121 ggacttttaa tatttgtgtg ctgctgccgg aagacgatta tttaaaggat atgccgtttt
      181 ttcagaaagt gcttcttgga atcagtgaat atttatccgg agcggattat gatgcaattg
      241 tagcaacggc ttcggaacat aatattgaaa atctgaaaag gatcgtgaca aataaaaagg
      301 tcgacggagt tgttctgaca cggaccgtaa aaaatgatcc tgcggtattg tttttgcaga
      361 agcagaagat cccgtttgtc gtaacgggcc tgtatgaggg agcggatgtc gttcaggtgg
      421 atgcaaggca tgaagaagca gcctgtgagc tgaccagata tctgttggga aaaggattga
      481 aaagaatttc actgcttggc gggaattccg ggcatatcgt cagccaaaag cgtcttcttg
      541 gagtagagca ggcatatgag atcatgcgga tcgatccgaa atatctttcc gtttatgaga
      601 atatgaacag cgagagtgac attacgcggg cgatgcagac tatcctgtat gagagctgtg
      661 actgcattct atgcgcggac gacagaatta cgatctcggc actgaataag cttcggaagg
      721 caaatgtgaa gatcccggag gagatcaggg tcgcaacgct ttttgaaagc gtgattttaa
      781 ataattatgt cccggccatt acggggctga cttatgatgt catgcagctt ggcaggatcg
      841 tggcagaaca gatcctgctt ctgatccagg gaaaacaagt ggaaaaactt acgacgctgg
      901 attatcagat cgtcataaag gagtcgacgc gctgattgct gttaacgggg aaaacgatat
      961 ggtaaaaaag aaaaagacca gtatggaata ccgctattat gatatgccgc cggacgagcc
     1021 tgtgctggcc ttgctcgggg acggatggaa aagaaaatac ggagaaggaa tcaagtttct
     1081 ccattttcat aatattcttg aaatcggcta ttgtcattat ggaaggggag agctgacata
     1141 tgatgtgatg gaagatgaac tgctgtgcag aaagtatgcg ggaggggaag tctctgttat
     1201 tccgagaagt attccgcata cgacgaacag cgcaccgggg tctgtctgct attgggaata
     1261 cctgtttgtg gatgtaacta attttcttgg agaatatacg gaaaaaaatc cgatacaggc
     1321 ggaaaaagtt atagagcgta tcaatgcaag accgcttttg ataaaagaag aggagcatac
     1381 actcctgact gatgccgtga aaggaatact ccgggtacaa aaggaaaaac agccgtatta
     1441 catggaaata acaaacggtc ttgtttttac aatgctgatg ctgatcgcac aggcgaaccg
     1501 cgcatacagt aagcagaatg aagaaagcag cagtgttt
//
LOCUS       DAOSYE010000628         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72657_length_1537_cov_2.936572, whole genome shotgun sequence.
ACCESSION   DAOSYE010000628 DAOSYE010000000
VERSION     DAOSYE010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72657_length_1537_cov_2.936572"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLU43_09985"
     CDS             complement(<1..396)
                     /locus_tag="PLU43_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28784.1"
                     /translation="MKKKVISMMLTIAMTASLLAGCGSTAETESETTAETAETTESAS
                     TDTTQETGNEVFDAAEETASETEEGEPVYGGTLRYASHNTVSTPGYTPECTNNASLIY
                     LNTAYESLIYYNETGDIIPRLATEWETDSE"
     gene            complement(646..>1537)
                     /locus_tag="PLU43_09990"
     CDS             complement(646..>1537)
                     /locus_tag="PLU43_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28785.1"
                     /translation="PNVSAKILRKGESQKYIIALFWNFDFRSVMMNRFLFGLQKRIRE
                     ENAEMNIVIHPYQTGELKKEEISFLCGEFHAAIIGNANKDDLEYLKKSSFRMPIILYN
                     RVLDGYCSVNVDNVKIGIMAAEHLYQQGYRKPAVVHGTKNFPGATLREEAFLSRMKEL
                     GVELPSKKLLYAENSLKGGYQCGETLKENIKDYQTDSFFCSSDSIALGIMNALSKEEL
                     IPYKLGIIAVGNGDPQYGMYYEPPLTVINIPIEQMATECYSFLNEGIYTMNTCADQRY
                     FETELFARKSTNKNGMNADL"
BASE COUNT          398 a          349 c          244 g          546 t
ORIGIN      
        1 ctctgaatct gtttcccatt ccgttgcaag ccttgggata atatctcctg tttcattata
       61 atagatcagt gattcatatg cagtattcag atagatcagt gatgcattgt ttgtacattc
      121 cggtgtatat cccggtgtgg aaaccgtatt atgagaagca tatcttaacg ttcctccata
      181 aaccggttct ccttcttctg tttccgatgc agtctcttct gccgcatcaa aaacttcatt
      241 tcctgtttcc tgcgtcgtat ccgtactcgc gctttctgtt gtttctgctg tttctgctgt
      301 tgtttcgctc tcggtctctg ccgtgctgcc gcagccagcc agaagggacg cggtcatagc
      361 gatcgtcagc atcatactaa tgactttttt cttcattttc ttcctcccaa taaaaattta
      421 taattagtta caacgttgta ctataacgtt gtactaataa tacagtataa tattgcatat
      481 gtcaatatta ttattttctt ttctttttag ttttttgtct aaataatctg ttctcttttc
      541 gccgacaatt tattattgtt ataaatatac aattttaatc tctaaatatt atacaatata
      601 cacaaaagga gtgtctgccc tgcagacact ccttttttaa tttacttaca aatcagcgtt
      661 cattccattt ttattagttg actttcttgc aaacagttct gtttcgaaat atctttgatc
      721 ggcacaggta ttcatcgtat atattccttc gttcaaaaac gaataacatt ctgttgccat
      781 ctgttcgatc ggaatattga tcactgtcag cggcggctca taatacatgc catactgcgg
      841 atctccgtta cctacggcaa tgattccaag tttatatgga atcagttcct ctttcgacag
      901 tgcattcata ataccgagtg caatggaatc agaggaacaa aaaaaactgt ctgtctgata
      961 atcttttata ttctctttca gcgtttctcc gcactgataa ccgcccttta aagaattttc
     1021 agcataaagc agcttttttg acggcaactc tacgcccagt tctttcattc tgcttaaaaa
     1081 agcctcttca cgaagcgtag ctcccggaaa attttttgtt ccatgaacga ccgccggttt
     1141 tcgatacccc tgctgataaa gatgctcagc tgccataata ccgatcttca cattatctac
     1201 attgaccgag cagtatccgt caagtactct gttataaaga atgatcggca tccgaaaact
     1261 gctttttttc aaatattcca gatcgtcctt atttgcattt ccgataatgg ctgcatggaa
     1321 ttctccgcac aaaaaactga tttcttcttt tttcagctcg ccggtctgat acggatgaat
     1381 aacgatattc atttccgcat tttcttccct gatccttttt tgcagaccaa acaaaaaccg
     1441 gttcatcata acgcttctga aatcgaaatt ccagaacagg gcaatgatat atttctggga
     1501 ttctcctttt ctcaatatct ttgcagatac atttgga
//
LOCUS       DAOSYE010000629         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72708_length_1536_cov_3.409858, whole genome shotgun sequence.
ACCESSION   DAOSYE010000629 DAOSYE010000000
VERSION     DAOSYE010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72708_length_1536_cov_3.409858"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..>1536
                     /locus_tag="PLU43_09995"
     CDS             108..>1536
                     /locus_tag="PLU43_09995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO28786.1"
                     /translation="MLFNSIQFLIFFPIVVLLYFLIPHKFRYVWLLFASYYFYMAQEP
                     SYAVFLVLTTVITWTAGNLLYRAVNKFQKNIIVAAGFLLTLSFLLYFKYTDFFLSLFG
                     SGRQLHLLLPLGISFYTFQSLTYIMDCYRQETPPEKNILKYALFVSFFPNILSGPIER
                     SKNLLKQFEQVHAFDVTRVKEGLTLMLWGYFLKMVLVSRLAILTDLVYDHYNIYTGMP
                     LIIATLSYTFQIYCDFAGYSSIAVGAARIMGFQIMQNFRQPYFAVSVSDFWHRWHISL
                     STWFRDYLYIPLGGSRCSKGRKYFNIAVVFLTSGFWHGANITFILWGALNGLYQIAGD
                     MLRPFRTYLCSITSLTKHPVLHRILRTIFTFLLLSITWVFFRAPTITDAGIIMKKMFT
                     TIDPADFINGTIFTLGLGTANLLFVVFSLLILLAADLLCEYRRCDISRLLIRTPVLLR
                     WGIYYLLFTMILLSCNLSTQEFLYMQ"
BASE COUNT          356 a          368 c          310 g          502 t
ORIGIN      
        1 tgacactgtt tcttcattat agtacaatca aggtcagaat acaagttcgc aaatgtgcgg
       61 gatacatact gcctttccat atggcggcag cttggcggag gatattgatg ctttttaatt
      121 ccatacaatt cttaattttt ttcccgatag ttgtactgct gtattttctg attccgcata
      181 agttcagata tgtatggctt ctttttgcaa gctattattt ctacatggca caggagccct
      241 cctatgcggt tttcctcgtt cttacgactg tcattacatg gacggcgggc aatctgctct
      301 accgtgccgt aaacaaattc caaaaaaaca tcattgtggc ggcaggattt ctgctgacgc
      361 tttcttttct gctctatttc aaatacactg attttttcct gtcccttttt ggcagcggca
      421 gacagctgca cctgctgctt ccgcttggta tctcctttta tacgttccag tctctgacat
      481 acattatgga ctgttatcgt caggaaacac caccggaaaa aaacatttta aaatacgcac
      541 tgtttgtctc cttcttcccg aatattctgt caggtccgat cgaacgctca aaaaacctgc
      601 tgaaacagtt tgagcaggta catgcatttg atgtaacgcg ggtaaaagaa ggactgacac
      661 tcatgctttg gggctatttt cttaaaatgg tccttgtttc ccgtcttgcc attctgacag
      721 accttgtcta tgatcattac aatatttaca ccggcatgcc gcttattatt gccacgctca
      781 gctatacgtt tcagatctat tgtgattttg cagggtattc gagcatcgcg gtcggcgcgg
      841 ccagaatcat gggctttcag atcatgcaga atttccggca gccttatttt gccgtcagtg
      901 tgtccgactt ctggcaccgc tggcatattt cactatctac atggttccgt gattatctgt
      961 atattcccct tggcggcagc cgctgttcaa agggcagaaa atactttaac attgccgttg
     1021 tatttttaac aagcggattc tggcacggag ccaatatcac cttcattctc tggggtgcac
     1081 tgaacgggtt ataccagatc gccggtgaca tgctgcggcc ttttagaaca tacctctgca
     1141 gcataacgtc acttacaaaa catccggtac tgcaccgtat actgcggacc atatttactt
     1201 ttctgctgct cagcatcacc tgggtctttt tccgcgcacc gactataaca gatgccggta
     1261 ttattatgaa aaaaatgttt acgacaatcg atcccgcaga ttttataaac ggcactattt
     1321 ttacactcgg acttggtacg gcaaatctgc tgtttgttgt tttttcactc cttatcctgc
     1381 ttgcagccga tttattatgc gagtaccgcc gttgtgatat cagccgcctg ctcattcgca
     1441 cgccggtcct gcttaggtgg ggtatttatt atcttttatt tacgatgatc ctgttatcgt
     1501 gtaatttatc aacacaggaa ttcctgtata tgcagt
//
LOCUS       DAOSYE010000630         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72757_length_1536_cov_2.137070, whole genome shotgun sequence.
ACCESSION   DAOSYE010000630 DAOSYE010000000
VERSION     DAOSYE010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72757_length_1536_cov_2.137070"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLU43_10000"
     CDS             complement(<1..725)
                     /locus_tag="PLU43_10000"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOO28787.1"
                     /translation="MLNIRVEKTASPKPLPSADNPLTFGTIFTDHMFMMDYKAGTGWY
                     DERIVPFQKLSLSPASMVFHYGQEMFEGLKAYKAEDGRILLFRPDKNIERANNTNKRI
                     CIPEIPADDFLQAIEETVLTDQAWIPTKPGTSLYIRPFIIATDPFLGVRPSHTYIFMI
                     ILSPVGAYYPEGLNPVKIWIEDEYVRAVRGGVGEAKTGANYVASLAAQVKAHEEGYSQ
                     VLWLDGVERKYIEEVGAMNIFFK"
     gene            954..>1536
                     /locus_tag="PLU43_10005"
     CDS             954..>1536
                     /locus_tag="PLU43_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28788.1"
                     /translation="MNFLVKIKDLLYKDVSSENELKEMAVLLRVLCITDAVFYTISAI
                     VLFLIFGSKEGVFSLVFVLIMLIILRTTYKNSTRISLFLYSFILAMIVIFYCALFGTQ
                     IGYQFSLYTVILLFFYKTDESTAFKMSSSIVCGLLAVVTSVYIDIVGPVNQLKNSQEV
                     VLLCIYSLWLLSKIIIISRFYCLKFSASEDKIIQ"
BASE COUNT          410 a          338 c          266 g          522 t
ORIGIN      
        1 atcttaaaga aaatattcat ggcgccgact tcctcaatat actttctttc cacaccgtca
       61 agccaaagca cctgcgaata tccttcttca tgcgctttta cctgtgccgc aagagaagcc
      121 acataattag caccggtttt agcttcgccg acaccacccc tgacagctct gacatactca
      181 tcttcgatcc atatcttaac aggattcagg ccttccggat aataggctcc gaccggtgaa
      241 agaatgatca taaatatata tgtatgggat ggtctgacac caagaaacgg atcagtggca
      301 ataataaaag gcctgatata aagagaggtt cccggttttg tcgggatcca cgcctgatcg
      361 gtaagaacag tctcctcaat agcctgcaga aaatcatcgg ccggaatttc cgggatacag
      421 atccttttat tcgtattatt tgctctttca atattcttat cgggacggaa cagaagaatc
      481 ctgccgtctt ccgccttata cgcttttaat ccttcgaaca tttcctgtcc ataatgaaat
      541 accatggaag caggagataa tgacagcttc tgaaaaggga cgattcgctc atcataccat
      601 ccagtccccg ctttataatc catcataaac atatgatccg taaaaatagt accgaacgta
      661 agcggattat ctgcactcgg caatggtttt ggtgatgctg ttttttcaac tctgatgttt
      721 aacatagtac tctccctttc tctctgttca tacaaatttt ctgcctgttt ttctgcctta
      781 ggcaattatg ttttattatt atacttttaa tttacaatgt caagtattta tgatatactc
      841 agatttagat attttgttca aaatcaaaag attttttcca ccctgtttgt ctattttttt
      901 taacgaaact ttgtttaatt tagtttaatt tatgaaatgt ttgggagaat gccatgaatt
      961 tcctggtaaa aataaaagat ttattatata aagacgtttc ttccgaaaac gaactcaagg
     1021 aaatggccgt actgctgcgt gtactatgta ttacagatgc ggtcttttat acaatcagcg
     1081 ccattgtact ttttctcata tttggttcga aggaaggtgt tttttctctt gtctttgtcc
     1141 ttatcatgct tataatcctg cgcactacat acaaaaactc aactcgtatc tctctgtttt
     1201 tgtattcttt tatccttgcc atgatcgtga ttttttattg tgccctgttc ggtacacaga
     1261 tcggctatca gttttccctc tatacggtaa ttttactttt cttttataaa accgatgaat
     1321 ccacagcgtt taaaatgagc tcttccatcg tgtgcggcct gctggcagtc gttacgtctg
     1381 tctacatcga tatcgtcggg cctgtcaatc agctgaaaaa cagccaggaa gtagttcttc
     1441 tctgtattta ttccctatgg cttctaagca agatcattat catatcccgc ttctactgtc
     1501 tgaagttttc tgcttccgag gataagatca tccagt
//
LOCUS       DAOSYE010000631         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72828_length_1535_cov_2.632432, whole genome shotgun sequence.
ACCESSION   DAOSYE010000631 DAOSYE010000000
VERSION     DAOSYE010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72828_length_1535_cov_2.632432"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PLU43_10010"
     CDS             <1..1356
                     /locus_tag="PLU43_10010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO28789.1"
                     /translation="EEKNAYRKKVYLSILIFDCLFLALFKYSDFFYQIYLAAGKTVHM
                     DFSNQLLERIVIKSAALAPFRISYFSLIMIGYITDVYWGKCIPQKNLGKLVLFTGYFP
                     QMTSGPIVQYETMQKTLWGEKHKFSYTEFMLGMERILWGVFKKLVISERCALVVNTVY
                     SYYEVYAGFYIPLAAGFFVLQLYTDFSGLMDIVLGFSQILGISLPENFQTPFYSVSLS
                     EFWRRWHITLGGFLRDYVFYPIQRSNKWRKLRKFCKKRFGKNYERKFNLPVYLALFAS
                     WFLIGLWHGGGWNYIFGVGLYMWLIIVLGEIFRPVFEWLIKVLKINTECASWRLFQRI
                     RTLFLFVFGLSFFRAENLTEGVRMWKSAFSLYNPWIFFDQSIYQLGLDKTEFQICLIA
                     TGILFLVSYFQQKESVRTQLSRQNFVFRLGVFAILLLLVITWGYYGTDYNASDFIYGR
                     F"
BASE COUNT          411 a          237 c          336 g          551 t
ORIGIN      
        1 gaagaaaaaa atgcatacag aaaaaaagta tatctaagca ttttgatttt tgactgcctg
       61 tttcttgcat tgtttaagta cagtgatttt ttttatcaaa tctatttggc ggcagggaaa
      121 acggttcata tggatttcag taatcagctg ttggagcgta ttgtaataaa atcagctgcg
      181 ctggcgcctt ttcgcatttc ctatttttct ctgatcatga ttggttatat tacggatgtt
      241 tactggggaa aatgtattcc tcagaaaaat cttggaaaat tggttctgtt tacaggttac
      301 tttccgcaga tgacctcagg accgatcgtc cagtatgaaa ccatgcagaa aacgctttgg
      361 ggcgaaaaac ataagttcag ttatacggaa tttatgcttg gcatggaacg tattttatgg
      421 ggtgttttta aaaagctggt tatttccgaa cgatgtgcgc tggtcgtaaa taccgtttac
      481 agctattatg aagtatatgc cggtttttat attccgctgg cagccggttt ttttgtcttg
      541 cagctgtata cggatttttc cggattaatg gatattgttc ttggattctc acagattctt
      601 ggaatttcgc tgccggagaa ctttcaaaca ccgttttatt cggtaagcct ctctgagttt
      661 tggagaagat ggcatattac actgggcgga tttttacggg attatgtatt ctatccgata
      721 caaagaagta ataagtggag aaaactgcgt aaattctgta aaaaaagatt tggaaaaaat
      781 tacgagagaa aattcaattt acccgtatat cttgctttgt ttgcttcctg gtttctgatt
      841 ggtctatggc atggcggcgg ctggaattat atttttggcg tcggtctgta tatgtggctt
      901 atcattgtat tgggggagat tttccgtccg gtttttgaat ggctgatcaa ggtattaaaa
      961 atcaatacgg aatgtgccag ctggagattg tttcagcgga tcaggacgct ttttcttttt
     1021 gttttcggat tgtcattttt cagagctgaa aatctgacag aaggcgttcg catgtggaaa
     1081 tcagcatttt ctttatataa tccatggatt tttttcgatc agtcgatcta tcagctggga
     1141 cttgacaaaa cagaatttca gatttgtctg attgcgaccg gtatcttatt tttggtctca
     1201 tattttcagc agaaagaatc tgtcagaacg cagctttcac ggcaaaattt tgtttttcga
     1261 cttggggtgt ttgccatatt gctgttgctt gtaattacat ggggttatta tggaacagat
     1321 tataatgctt ctgattttat ttatggaagg ttttaaaaat gagaataaaa aattttttta
     1381 aggcagtgct tgtcttcctt gtaatggcat ttgttatatt gatctgtgaa aaggttttac
     1441 tgctaaaatc tgaagacggc atcgaacaga tcaaatcatt ttatcttcaa aaagaaaata
     1501 ccattgatgt tttgttcctt gggagttccc atatc
//
LOCUS       DAOSYE010000632         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72879_length_1534_cov_3.279919, whole genome shotgun sequence.
ACCESSION   DAOSYE010000632 DAOSYE010000000
VERSION     DAOSYE010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72879_length_1534_cov_3.279919"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..948)
                     /locus_tag="PLU43_10015"
     CDS             complement(10..948)
                     /locus_tag="PLU43_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28790.1"
                     /translation="MSELMVRLLPVFILLVIGFISRKSGLFRESFVDGLKTLIVKIAL
                     PAVLFNSFSTMELQLSYLLLFAMVFLYCALLYGLGNLLHRVFPSVFGRLYTKGFMTGF
                     EFGMIGVGLFGAIWGMDKLPVIMLIGFGHELFIWFCYVPLISANEKEKFNLGKTVMEF
                     LKTPTIAAILIGVAVNLLGLYPVLEGNVIGKSVLETVGLLTPLTSPLILIVIGYNMSF
                     ARGGLKEITAYLSARILLTLGIGTLVFFLINSMVHGLDPLFTEAFYAFLLLPAPYILP
                     LFISEQEEAAFFTQLLVYSTLLTFIGYGVLAWFSAI"
     gene            complement(1049..1534)
                     /gene="fucI"
                     /locus_tag="PLU43_10020"
                     /pseudo
     CDS             complement(1049..1534)
                     /gene="fucI"
                     /locus_tag="PLU43_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247880.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose isomerase"
BASE COUNT          470 a          396 c          324 g          344 t
ORIGIN      
        1 aaaaatacat catatagcgc taaaccacgc cagcactcca tatcctatga atgtaagaag
       61 cgtggaatac acaagaagct gcgtgaagaa cgcggcttct tcctgttcac tgataaaaag
      121 cggcagaata taaggggccg gcagcagcaa aaacgcataa aaagcttccg taaataatgg
      181 atcaagccca tgtaccatac tgtttatcag aaagaaaacg agcgtgccga tcccaagggt
      241 aagcagtatc cgcgcagaaa gatacgctgt gatctcctta agcccccctc gtgcaaagct
      301 catattatat ccgatcacga tcaggatcag cggagaagtc agcggtgtta aaagaccgac
      361 tgtttccagt acgctctttc cgatcacgtt tccttccagc acaggataaa ggccgagaag
      421 attgactgcc acaccgatca ggatagctgc gatcgtcggt gtcttaagga attccataac
      481 tgtttttccc aaattgaatt tttccttttc atttgcggaa ataagcggca catagcagaa
      541 ccagataaac agttcatgtc caaaaccgat cagcataatg accggaagct tatccattcc
      601 ccagatcgca ccgaacagtc cgacaccgat catcccgaac tcaaatcctg tcatgaatcc
      661 tttcgtataa agtctcccaa aaaccgacgg aaaaacccta tgaagcagat ttccaaggcc
      721 atagagcaat gcgcagtata aaaataccat ggcaaaaaga agcagatagg acagctgcag
      781 ctccatcgtt gaaaaagaat tgaaaaggac cgccggcagg gcgatcttga cgatcagcgt
      841 ttttaatcca tcgacaaagg attccctgaa cagtccgctt tttctggaaa taaaaccgat
      901 cacaagcaaa ataaataccg gcagaagccg gaccatcaat tctgacatct tttctgccct
      961 cgctttttaa gtatacagac attttcaatg cctgcatact tattatatgc gaagagatca
     1021 tatttgtaaa acagcaaaaa gaaatgggtt atttgtataa tggtccgtat ttttcacaga
     1081 cacgaaaatc agtcgattcc agattttccg ttccaaagga actccataca tggggcctga
     1141 atatccgctc ccatgcaaca ttatgcatgt tcacgggaat acggagcatg gacgccagcg
     1201 tgatcagttc cgccccgata tggccgtaag cgcatgctcc atgattcgct ccccagtttg
     1261 ccataacgga atacacgctg tcaaaagcac ctgttccggt aagacacggt acaaaccatg
     1321 tcgtcggcca ggtcggatcc gtcctcttat ccagtgtttc atgcaccgcc tccggaagcg
     1381 tcacgctgaa gccttcggca agctgcagca cgggaccgat cccgtctacg atattcatcc
     1441 ttaagaatgt gagcggcatg accgcagatg tctgaaagtg agaagaaatc caccgcccct
     1501 gaactggcag agttcggccg gacaccagtc cact
//
LOCUS       DAOSYE010000633         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72900_length_1534_cov_2.675456, whole genome shotgun sequence.
ACCESSION   DAOSYE010000633 DAOSYE010000000
VERSION     DAOSYE010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72900_length_1534_cov_2.675456"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLU43_10025"
     CDS             <1..541
                     /locus_tag="PLU43_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28791.1"
                     /translation="KIQYARGNETVYVCGSYKRYTKAVCSRHEVSQTDLEKAIIDKLN
                     EYIAKSDIIINKTDTASEINLESIKKYNDLMNIVKIRKKKLFETLSDGLISKEEYTEY
                     KETYDTQEKQYLAMIESIEQENKQENSDKMIWIKTLQTKKKIDKLDRSILADTVEKIT
                     VTETDDTVHVDIRFRFSLD"
     gene            571..>1534
                     /locus_tag="PLU43_10030"
     CDS             571..>1534
                     /locus_tag="PLU43_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HOO28792.1"
                     /translation="MAYIKAGIGGEFIPATNELCIRFAEHFKYRQTVGGTATRAAIAI
                     SRLGCESSLSMCCFNEQIRRLLPQKIHYFSNVGDASQTIYPHVILSYPAGERLCVNNI
                     DMITSRENRLMYSNDDDAKSMMVSAEFSSWLTDADAFLLGCFSEVLDRNTLKERMEST
                     GSLFRSMKDSAWIVLEDGCYIKKEFRYYVHQSLRKFLDVISMNEDELQEYIEKRIDVF
                     DYRAVLEEISVVYGRLGVPVLVVHSAGWALAYGERPERLKAALYRGICLSATRFRYGD
                     DFDKKEYEKTERLAPKAEGAEFCRNIKKLAGDKICCLPTKDLSFV"
BASE COUNT          502 a          257 c          378 g          397 t
ORIGIN      
        1 aaaaatacaa tatgcaagag gcaatgagac tgtttatgtt tgtgggtcat acaaaagata
       61 tacaaaagct gtatgcagta gacatgaggt cagtcagaca gatttagaaa aagccatcat
      121 tgataagttg aatgaatata ttgccaagtc agacatcatt atcaataaaa ctgatactgc
      181 atctgaaatc aatttggaga gtattaagaa gtataatgac cttatgaata ttgttaagat
      241 taggaaaaag aaattgtttg aaacattatc agatggacta atttccaagg aagaatatac
      301 agagtacaaa gaaacctatg atacacagga aaaacagtac cttgcaatga ttgaatcaat
      361 tgaacaagag aataagcaag aaaacagtga caaaatgata tggataaaaa ctttgcagac
      421 caagaaaaaa attgataaac tggataggag tattcttgcg gatactgtag aaaagattac
      481 tgtaactgaa acagatgata cagtgcatgt tgatatcaga tttaggttta gtttagattg
      541 agaatagggc aatcctaatt gagcattacc atggcctaca tcaaggccgg aatcggcggc
      601 gaatttatcc ctgcgaccaa tgaactttgt atccggttcg cggagcattt taaatacagg
      661 caaacggtag gaggaacggc gacacgggcc gcgatcgcca tcagcaggct tggctgtgag
      721 agttcacttt ccatgtgctg tttcaatgaa cagatccgca ggctcctgcc gcagaagatc
      781 cattattttt caaacgtagg ggatgcttcg caaacgatct atccgcatgt gattctttct
      841 tatcctgcag gagaacgcct ctgtgtcaat aatattgata tgattacttc gcgggaaaac
      901 aggctgatgt attcaaatga cgacgatgcg aaaagcatga tggtttccgc agagttttca
      961 tcatggctga cagatgcgga tgcgtttctt ctcggatgtt tcagcgaagt gcttgatcgt
     1021 aatacgctaa aagaacgcat ggaaagcacc ggcagcctgt ttcggagtat gaaagacagt
     1081 gcgtggatcg tgttggagga tggctgttat ataaaaaagg agttccggta ttacgtgcat
     1141 caaagcctgc ggaaatttct cgatgtcatt agtatgaacg aagacgagct gcaggagtat
     1201 attgaaaaaa gaatcgacgt atttgactat cgggctgttt tggaggagat ctccgttgtt
     1261 tacggcaggc ttggcgttcc cgtactggtc gttcattcgg ccgggtgggc gcttgcatac
     1321 ggagagcggc ctgaaagact gaaggcagca ttgtacaggg gaatttgcct gtctgcaacg
     1381 cgtttcaggt acggagatga ttttgataaa aaagagtatg aaaaaacaga aagacttgct
     1441 ccgaaagcgg agggagcgga attttgcaga aatattaaaa agttagcagg agataagata
     1501 tgctgtctcc cgacaaaaga tctgagtttt gtta
//
LOCUS       DAOSYE010000634         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72913_length_1534_cov_2.315078, whole genome shotgun sequence.
ACCESSION   DAOSYE010000634 DAOSYE010000000
VERSION     DAOSYE010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72913_length_1534_cov_2.315078"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLU43_10035"
     CDS             <1..474
                     /locus_tag="PLU43_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO28793.1"
                     /translation="MTSNAGAGSIIDPKKLGFAAVSDEKHDYEKMKSGVMEEVKHIFK
                     PEFINRIDEIIVFHALNEDNMKKIVTLLVKNLAKRAKEQMNITLTVRDTVKKYIVEKG
                     ADKKYGARPLKRAIQTMLEDKLAEEILEGHIREGQQVIVSLIKKDGKNEIKMTGK"
     gene            534..944
                     /locus_tag="PLU43_10040"
     CDS             534..944
                     /locus_tag="PLU43_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HOO28794.1"
                     /translation="MSVVEELIRTEEDGALSFGNHELPRKAKVEDYECAGNLYKVKTF
                     YEMTKLERDGMFVYESVPGTSVSEFKASAKGVEFSVCGAEDAQITIGLKEDTEYEVFV
                     SEKSVGKMKTNLGGKLNISVELAGEGEIPVRIVE"
     gene            952..>1534
                     /locus_tag="PLU43_10045"
     CDS             952..>1534
                     /locus_tag="PLU43_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOO28795.1"
                     /translation="MAKGKLSTVFYCSQCGYESAKWMGQCPACREWNTFVEETVSKTV
                     NGKRESGSHRTGRKLPEKITAIRLTEDEKVMTHIEELDRVLGGGIVPGSLVLVGGDPG
                     IGKSTLLLQVCKNLSDDGHQVLYISGEESLKQIKMRAERIGQFNDCLSLFCETNLSDI
                     EEVIRREKPEAAVIDSIQTMFCEEVSSAPGSVSQ"
BASE COUNT          491 a          243 c          438 g          362 t
ORIGIN      
        1 atgacatcga atgcgggggc aggcagtatt atagatccga aaaaacttgg gtttgcggct
       61 gtcagcgatg aaaagcatga ttatgaaaag atgaagtcag gtgtcatgga ggaagtaaag
      121 catattttca aaccggaatt tattaaccgg atcgatgaaa ttattgtgtt ccatgccctg
      181 aatgaagata atatgaagaa gatcgtaaca ctgctcgtaa agaacctggc aaaacgtgcg
      241 aaggaacaga tgaatattac gctgactgtc agggatacgg tgaaaaaata tattgtagag
      301 aaaggagctg ataaaaaata cggtgcgaga ccattaaaac gtgccattca gacgatgctt
      361 gaagataagc tggctgaaga aatactggaa ggacatatca gggaaggaca gcaggtcatt
      421 gtctccctga tcaaaaaaga cggaaaaaat gaaattaaaa tgactggaaa ataagcggga
      481 tatagagtat actatttaaa gataacacat tatatgtagg aggatcaaac atcatgtctg
      541 tagtagaaga actgattcgg acggaagaag acggcgcttt aagttttggt aatcacgagc
      601 ttccaaggaa ggcaaaggtg gaggactatg aatgtgccgg aaatctctat aaggtaaaga
      661 ctttttatga aatgacaaaa cttgagcggg atggcatgtt tgtatatgaa tccgtaccgg
      721 gtacgagtgt cagcgagttc aaagcatcgg caaagggtgt ggaattttca gtgtgcggtg
      781 cggaggatgc acagattacg atcggtttga aagaagatac ggaatacgaa gtatttgtat
      841 cggaaaaaag tgttggcaag atgaagacaa atcttggcgg caagctgaat atcagcgttg
      901 agcttgcagg agaaggagag attccggtca ggattgttga ataggagaag catggcaaag
      961 ggaaagcttt cgacggtatt ttattgcagc cagtgcggct atgaatctgc caaatggatg
     1021 ggacagtgtc ccgcctgcag ggaatggaat acgtttgttg aagagacagt cagtaagacg
     1081 gtaaacggaa agagagagtc gggatctcac agaacggggc ggaagctgcc ggaaaaaatc
     1141 acggcaattc ggcttactga agatgagaaa gtcatgactc acatagaaga gctggacaga
     1201 gttttgggag gcggtatcgt accgggctcc cttgttttgg taggaggaga tcccggtatc
     1261 ggtaaatcga cacttctttt gcaggtctgt aagaatcttt ctgatgacgg acatcaggta
     1321 ctgtatattt caggcgaaga gtcgctaaag cagatcaaga tgcgtgcgga gcgtattgga
     1381 cagttcaatg actgcctttc tttgttttgt gagacaaatc tgtctgatat tgaagaggtc
     1441 atacgaaggg aaaagccgga ggcggctgtg atcgattcga tccagacaat gttctgtgag
     1501 gaggtttcct cggctccggg cagtgtttcc cagg
//
LOCUS       DAOSYE010000635         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_72962_length_1533_cov_2.809878, whole genome shotgun sequence.
ACCESSION   DAOSYE010000635 DAOSYE010000000
VERSION     DAOSYE010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72962_length_1533_cov_2.809878"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="flhB"
                     /locus_tag="PLU43_10050"
     CDS             complement(<1..1046)
                     /gene="flhB"
                     /locus_tag="PLU43_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOO28796.1"
                     /translation="MVNYDLQRFAKDGPGGEKTEPATEKKLRDARTEGQVAKSHELAN
                     AVGLMILFLVLKIWAGSMGTKFLENFYYVYERIPEMVTLVNGKITPKLFASMLNQMIL
                     RMLFLLLPVFALGFTAAFGIDLMQVKWQPTTKPLAPKGSKLNPLKGFKRIFSKDKLVE
                     LLKSVIKLLLIGYMAVTTLKDEVTSLFLLYDMTLLSAIKLFGNVAINLGIKISAFYLI
                     LGFADYLYQKWKFSEDMKMTKQEVKDEWKNAEGDPKIKQKQRQRMMEAPRRRMMQAVP
                     KADVVITNPTHFAVAVKYDPKVADAPYVLAKGEDFLAAKIKEEARKYHIDIVENKPLA
                     RMLYYNVELGAQIPP"
     gene            complement(1093..>1533)
                     /locus_tag="PLU43_10055"
     CDS             complement(1093..>1533)
                     /locus_tag="PLU43_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOO28797.1"
                     /translation="DPLTKETSGFTGTMYQYAVILLLMVTDMHHYILRALVETYTLIP
                     IGGAKFQSDKIMTTMVQFLSDYVMIAFRICLPVVAAMMILNAVLGILAKTAPQINMFS
                     VGIQLKILTGLTVLFLTISVLPSASEYIFKEMKIMMTAMVESMT"
BASE COUNT          410 a          396 c          294 g          433 t
ORIGIN      
        1 ggcgggatct gtgccccaag ctccacatta taatagagca ttctggcaag cggtttattc
       61 tctacaatat caatatgata ttttcgtgcc tcttccttga tcttcgccgc aagaaaatcc
      121 tctcccttgg caagcacata cggtgcatcg gcaactttcg gatcatattt gaccgcaacc
      181 gcaaaatgag tcggatttgt gatgacgaca tctgcttttg gaactgcctg catcatacgc
      241 cttctggggg cttccatcat tcgctgcctc tgcttttgtt tgatctttgg atccccttcg
      301 gcattcttcc actcatcctt gacttcctgc tttgtcattt tcatgtcttc actgaatttc
      361 cacttttgat aaagatagtc cgcaaagcca agtatcaggt aaaacgcact gatcttaata
      421 ccaagattga tcgcaacatt tccaaatagt ttgattgcac tgagcagtgt catatcgtaa
      481 agcaggaaca gggatgttac ctcgtctttg agcgtcgtga ccgccatata gccaatcagc
      541 aaaagcttga tcaccgattt taaaagctct acaagcttat ccttggaaaa gatcctttta
      601 aatcccttca gcggattcag cttgctgcct tttggcgcca gcggttttgt agtcggctgc
      661 cattttacct gcataagatc aattccaaat gccgccgtaa agccaagtgc aaaaaccgga
      721 agcagcaaaa aaagcattct taaaatcatc tgattcagca tacttgcgaa aagcttcggc
      781 gtgatcttgc cattgaccaa agtaaccatc tccggtatcc gttcgtaaac ataatagaaa
      841 ttctccagaa attttgttcc catacttccg gcccatattt tcagtacaag aaacaatatc
      901 atcaggccga ccgcattggc aagctcatgg cttttggcca cctgaccttc ggttcttgca
      961 tcccgcagtt ttttctcggt tgcaggctct gtcttttccc cgcccggacc gtcttttgca
     1021 aatcgctgca gatcatagtt taccatttgt ttcccgcttt cctgtacatg cggcagaagc
     1081 catataaccc tgctaggtca tactttccac catagccgtc atcatgatct tcatttcctt
     1141 gaaaatatac tcagaagcac tcggaagtac gctgatcgtc aaaaacaaaa cagtcaggcc
     1201 cgtcaggatc tttaactgaa taccgacgga aaacatattg atctgcggcg ccgtcttagc
     1261 cagaatacca agtacggcat ttaatatcat catagccgct acgaccggca gacagatccg
     1321 aaatgcgatc atgacataat ccgacagaaa ctgcaccatt gtagtcatga tcttatccga
     1381 ctgaaacttc gcaccgccga tcggtatcag cgtatacgtt tcaacaagag cacgcaaaat
     1441 ataatgatgc atatcggtaa ccatcaagag aagaatgact gcatattgat acatggttcc
     1501 agtgaacccg gaggtttctt ttgtcagcgg atc
//
LOCUS       DAOSYE010000636         1532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73013_length_1532_cov_3.376439, whole genome shotgun sequence.
ACCESSION   DAOSYE010000636 DAOSYE010000000
VERSION     DAOSYE010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73013_length_1532_cov_3.376439"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLU43_10060"
     CDS             <1..701
                     /locus_tag="PLU43_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOO28798.1"
                     /translation="PDCIRKLKDLVIDEPRISVKALKTKYPQGSERHLIYATTRRAIN
                     SSMLPADAGCIVDNVDTIVAIYHAVIEGRPLMHRIVTVTGDAISSPQNFLVRTGTSYA
                     QLVDEAGGFKVKPEKIVSGGPMMGFSIFDVNVPTTKTSSALLCMTTDEVAKWTPTHCI
                     NCGKCVEVCPGRIIPSEVADFAEHYDEAAFLKYNGMECCECGCCSYVCPAKRHLTQSI
                     KSMRKMVLANRKKK"
     gene            730..>1532
                     /locus_tag="PLU43_10065"
     CDS             730..>1532
                     /locus_tag="PLU43_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOO28799.1"
                     /translation="MSDLMSVSSTPHIRGKMSTSKIMFAVIIALLPATGVGIYNFGYH
                     AAVLVAVSIAACVLTELIYEKLMKLPVTVGDLSAVVTGLLLALNLPPALPWWIAAIGG
                     VFAILVVKMLFGGIGQNFMNPALGARCFLLICFTARMTNFTYDGITTATPLANLKSGI
                     AVNTWDMVIGTIPGTIGETSVVAIVAGAILLILTGVIDLKIPGTYLVTFAIFVGLFGG
                     HGWDPYYLTAQLAGGGLMLGAFFMATDYATSPITDKGKLLYGVMLGIMTG"
BASE COUNT          392 a          288 c          423 g          429 t
ORIGIN      
        1 aaccggactg catcagaaaa ttaaaggatc ttgtaattga cgagccgcga atcagtgtga
       61 aagcactcaa gacgaaatat ccgcagggct ctgaacggca tctgatttat gcgacgacga
      121 gacgtgcgat caattcctcc atgcttccgg cagatgccgg ctgtattgtc gataatgttg
      181 atacgatcgt tgcgatctat catgcggtca tagaaggaag accgctgatg catcgtattg
      241 taacggtaac gggagatgcg atcagttctc cgcagaattt tcttgtgcgt acgggaacaa
      301 gctatgccca gttagttgat gaggcgggcg gatttaaggt aaagcctgaa aagatcgtgt
      361 caggaggacc tatgatggga ttcagtattt tcgacgtgaa tgtaccgacc accaaaactt
      421 cctcagcact gctttgtatg acaacggatg aggttgccaa atggaccccg actcattgca
      481 ttaattgcgg aaagtgtgtt gaagtctgtc cgggcagaat cattccgtcg gaggttgcgg
      541 attttgccga gcattatgat gaagccgctt ttttgaagta taatggtatg gaatgctgtg
      601 aatgcggatg ctgcagctat gtatgtccgg caaaacgaca tttgacacaa agcattaaat
      661 ctatgagaaa aatggttctt gcaaatagaa aaaagaaata gacgagatta agagaaagag
      721 gtgtttgttt tgagtgattt gatgagtgta tcctcaacgc cgcatataag aggcaagatg
      781 tcgacaagta agatcatgtt tgcggtcatt attgcgctcc ttcccgcaac gggagttggt
      841 atctataatt tcggatatca tgcagcggtt cttgtggcag taagtatagc ggcgtgtgtg
      901 ctgacagagc tgatttatga aaaattgatg aagctgccgg taacggtcgg tgatctgagt
      961 gcggtcgtaa caggactgct tttagcgctg aaccttccgc ctgcgctgcc atggtggatt
     1021 gcagcaatag gcggtgtatt tgcaattctg gtagtaaaga tgctgtttgg cggtattggc
     1081 cagaacttta tgaatccggc gcttggtgcc agatgctttt tattgatctg ttttacggca
     1141 aggatgacga attttacgta tgacggtatt acaacggcaa cgccgcttgc gaatttaaaa
     1201 tccggaatag ccgttaatac atgggatatg gtgattggta cgataccggg cacgatcggt
     1261 gagacttctg tcgttgcaat tgtggcaggt gccattctgc ttatactgac aggtgtgatt
     1321 gatctgaaaa tacccggtac ttatcttgta acgtttgcga tatttgtcgg tctgttcgga
     1381 ggacatggct gggatccgta ttatctgacc gcacagcttg caggcggtgg tctgatgctg
     1441 ggtgcttttt tcatggcgac agattatgcg acttctccga tcacggacaa gggtaaactt
     1501 ctgtatggag tgatgcttgg tattatgaca gg
//
LOCUS       DAOSYE010000637         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73419_length_1527_cov_3.050272, whole genome shotgun sequence.
ACCESSION   DAOSYE010000637 DAOSYE010000000
VERSION     DAOSYE010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73419_length_1527_cov_3.050272"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLU43_10070"
     CDS             <1..793
                     /locus_tag="PLU43_10070"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO28800.1"
                     /translation="DNLDEQALKEAMTGCEFVFHFAANADVRMGCEHPGRDLEQNTIA
                     TYHILEAMRANGIKKIGFSSTGSVYGEAKMIPTPEDAPFPIQTSLYGASKLACEGLLA
                     AYAEGFDFQVYIFRFVSILGERYTHGHVFDFCKKLSDDPARLYILGNGKQRKSYLYVK
                     DCMEAILTVVANAEEKVNIFNLGTDEYCEVNDSVGWICEKLGLEPELSYAGGERGWIG
                     DNPFIYLDTKKVRSLGWEPKASIKEGIMKTVEYLQANPWVFQSRK"
     gene            835..>1527
                     /locus_tag="PLU43_10075"
     CDS             835..>1527
                     /locus_tag="PLU43_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOO28801.1"
                     /translation="MIIARSPLRISLGGGGTDLPSYYQEHEGFLISAAIDKYVYITLH
                     QTFEEAILLKYSRFEHVHEIDEIQHPIIREALKMLKWENKNIEISSMADIPAGTGLGS
                     SSSFTTALLRALHTMQGNIVSTRTLAEEACEIEMNRLHEPIGKQDQYIASYGGITCMN
                     FHKDGYVWVDPLNISKETLYNLEDNLILYFTGFSRSAGSILKEQDQKSKEKNDEMIKN
                     LDFVKDLGYQSKA"
BASE COUNT          451 a          274 c          389 g          413 t
ORIGIN      
        1 agacaatctg gatgagcagg cactaaaaga agcaatgaca ggctgtgagt ttgtattcca
       61 ttttgctgca aatgcagatg tcagaatggg ctgtgagcat ccgggaaggg atctggagca
      121 gaatacaatc gcgacttatc atatattgga agcaatgcgt gcaaacggta tcaaaaaaat
      181 cggattttct tctacaggtt ctgtttatgg agaagcaaaa atgattccga cgccggaaga
      241 cgcaccgttc ccgatccaga cttcacttta tggagcttcc aagcttgcct gtgaaggact
      301 gcttgccgca tatgcggaag gttttgattt tcaggtctat attttcagat ttgtttcgat
      361 tcttggagaa cgttatacac atggtcatgt gtttgatttt tgtaaaaagc tgtccgatga
      421 tcctgccaga ctttatatac ttggaaacgg aaaacagaga aagtcgtatt tatatgtaaa
      481 ggactgtatg gaagcgatcc tgacggtcgt tgcaaatgca gaggagaagg tcaatatttt
      541 taatcttgga acggatgaat attgtgaagt caatgattcc gtcggctgga tctgcgagaa
      601 gcttggttta gagccggagc tttcctatgc aggaggagaa agaggctgga tcggggataa
      661 tccgtttatt tatcttgata caaaaaaagt ccgttctctt ggctgggagc cgaaggcatc
      721 tataaaagaa ggtattatga aaacggtcga atacctgcag gcgaatccat gggtctttca
      781 gtcccgtaag taaacctggc cgggtaattt tttgatatag caggaggagt aaaaatgatt
      841 attgcaagaa gtccgcttag aatatcgctg ggaggcggag gtacggatct gccgtcttat
      901 tatcaggagc atgagggctt tttgatctcc gcggcaatcg ataagtatgt ctatattaca
      961 cttcaccaga cgtttgaaga agcaattctc ttaaaatatt ccagatttga acacgttcat
     1021 gaaattgatg agatccagca tccgatcatc cgagaggctt taaaaatgct gaaatgggaa
     1081 aataaaaata ttgagatcag ttccatggcc gatattccgg ccggtacggg tcttggttct
     1141 tccagcagct ttactacggc cctgctgcgc gcgcttcata cgatgcaggg aaatatcgta
     1201 agtacgagga cgcttgcaga agaagcctgt gagatcgaaa tgaatcgcct gcatgaaccg
     1261 atcggcaaac aggatcagta tattgcgtct tatggcggaa ttacctgtat gaacttccat
     1321 aaggacggat atgtatgggt ggatccgctg aatatttcga aggaaacgct gtataacctg
     1381 gaggataatc tgatccttta ttttacggga ttttcccgtt cggcaggcag tattttaaag
     1441 gaacaggatc agaaaagcaa ggaaaagaac gatgagatga ttaagaacct ggattttgta
     1501 aaagatctgg gatatcaaag caaggca
//
LOCUS       DAOSYE010000638         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73423_length_1527_cov_2.925272, whole genome shotgun sequence.
ACCESSION   DAOSYE010000638 DAOSYE010000000
VERSION     DAOSYE010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73423_length_1527_cov_2.925272"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="malQ"
                     /locus_tag="PLU43_10080"
     CDS             complement(<1..1000)
                     /gene="malQ"
                     /locus_tag="PLU43_10080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOO28802.1"
                     /translation="MRKSGILLPVASLPGKYGIGTFSKNAYHFLDCLKASGQSFWQIL
                     PLGPTGYGDSPYQSFSTFAGNPYFIDLETLIEEGYLKKSYCDRYDFGSDSGYIDYKKV
                     YESRFTVLWHAFEHSNYAENEEFHRFTEENAFWLDDYALYMAVKNSFGGRSFIEWDED
                     IRLRKIEAMTEYRNKYQKEVAFYQFQQFLFAQQWTRLKKYANSLDIKIIGDIPIYVAF
                     DSADTWANPKLFQFDKDRNPIAVAGCPPDAFSATGQLWGNPLYDWSYHKQTGYEWWIR
                     RIAHCFKLYDVVRVDHFRGFDEYYAIPYGDKTAISGKWMKGPGIDIFKKLNQALGEL"
     gene            complement(1032..1527)
                     /locus_tag="PLU43_10085"
                     /pseudo
     CDS             complement(1032..1527)
                     /locus_tag="PLU43_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520530.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
BASE COUNT          444 a          373 c          287 g          423 t
ORIGIN      
        1 cgagctctcc aagtgcctga ttcaattttt taaaaatatc aatgccaggg cctttcatcc
       61 atttaccgga tattgccgtc ttatctccat atggaattgc ataatattca tcaaatccgc
      121 ggaaatgatc aacgcggaca acatcataaa gtttaaagca gtgtgcgatc cgcctgatcc
      181 accactcata tcccgtctgt ttatgatagc tccagtcata aagtgggttg ccccaaagct
      241 gtcccgttgc cgagaaggcg tcaggcggac agcctgccac agcaatcggg ttccggtcct
      301 tgtcgaactg gaagagcttt gggtttgccc aggtatctgc gctgtcaaac gcaacgtaga
      361 tcgggatatc tccaattatt tttatatcaa ggctattcgc atattttttc agccgtgtcc
      421 actgttgtgc gaacagaaat tgctgaaact ggtaaaatgc gacttctttc tggtatttgt
      481 ttcgatattc agtcatcgct tctatctttc tcaaacgaat atcttcatcc cattcaataa
      541 aactgcgccc gccaaaggaa ttctttaccg ccatataaag cgcataatca tcaagccaga
      601 atgcgttttc ttcagtaaaa cggtggaact cctcattctc tgcataatta ctgtgttcga
      661 acgcatgcca cagcacagtg aaacgggatt catagacctt tttataatca atataaccac
      721 tatcgctccc aaaatcgtac cggtcacaat aggatttctt caaatatcct tcttctatca
      781 gtgtttccag atcaataaaa tatggatttc cggcaaatgt agaaaatgac tgatacggag
      841 aatcgccata tcctgttggc cccaggggaa gaatctgcca gaaactttgt cctgacgctt
      901 tcaaacaatc cagaaaatga tatgcatttt ttgaaaatgt tccgattcca tatttgcccg
      961 gcaggcttgc gaccggaagt aaaataccac tttttctcat aatggcctcc attttttccc
     1021 aatgccgtat ctcagccctt tacagcgcca gctacaacac cttttatgat atatttctga
     1081 catgccagat aaaatacaat gatcggaatg atcgcaagta cgagcatcgc catcatcgca
     1141 cccatgtcaa tagaaccata tccgcctttc aaatactgga tcgctattga aatcgtctta
     1201 tacttttttg tatcaagaac aagataagga agcagataat cattccagat ccacatggcc
     1261 tgcaggatcg cgaccgtaat actggtcggt ttcaggatcg gaaaaacgac ctgaaaataa
     1321 gtctgcagcg ggttacaccc atcgatcatc gcagcttctt caatttcaag cggaattgat
     1381 tttacaaatc cgcagaacat aaataccgca agccctgcac caaccaagat atacgacgat
     1441 gattgtgacc ggattggtca aatgcagcat gttcgcaatt tttgaaagtg tgaacatgac
     1501 catctgaaac ggaacgacac ggcccag
//
LOCUS       DAOSYE010000639         1523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73709_length_1523_cov_2.885559, whole genome shotgun sequence.
ACCESSION   DAOSYE010000639 DAOSYE010000000
VERSION     DAOSYE010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73709_length_1523_cov_2.885559"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLU43_10090"
     CDS             complement(<1..332)
                     /locus_tag="PLU43_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28803.1"
                     /translation="MEILEIDASNASIRLSYRVFIKDELYNVEEYFRIRRGSDRIYLM
                     EYERYMNQVFDESKNILVNGKLLHGILNEELQYEENSDASVYCFVQQNMLFSYNVTTN
                     SLAKLYSF"
     gene            complement(381..1028)
                     /locus_tag="PLU43_10095"
     CDS             complement(381..1028)
                     /locus_tag="PLU43_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28804.1"
                     /translation="MKKALIKLSVFLAVFLISVIVFSKIINRGNTDLTAEMSAATLPV
                     LYMNVNDEYVNCLHGYTVEMEGNYLRGALTPLQANRTVSFRADTYGAVISKVAYEVRS
                     MDMQRLIEDTVVTGFSYADDVITAEIPIKDLIEENTEYMLVIKLTTGAGKEIRYYTRI
                     IDEAEIYLNEKMEFVEEFSALTFDKEAAKSLVMYMESNSEGDNSSYGYVNIHSSF"
     gene            complement(1025..>1523)
                     /locus_tag="PLU43_10100"
     CDS             complement(1025..>1523)
                     /locus_tag="PLU43_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO28805.1"
                     /translation="ACSLLKDKYGYDKISFECGDIATYTKADRADMVVTLHACDTATD
                     YALYKAVQWGASVILSVPCCQHELNGQIESRLLEPVLKYGYIKERMSALFTDGIRAEL
                     LESQGYEVQVLEFIDMEHTPKNILIRAVKRQRELPRQKEMQELETLLKELHAAPRLME
                     LLEKL"
BASE COUNT          410 a          387 c          272 g          454 t
ORIGIN      
        1 cagaacgaat ataatttcgc caggctgttt gtcgtaacat tataactgaa cagcatgttc
       61 tgctgcacaa aacagtatac agaggcatcc gagttttcct catactgaag ctcttcattc
      121 aaaatcccat gcaaaagttt tccgttgaca agaatatttt tgctttcatc aaagacctga
      181 ttcatataac gttcatattc catcagataa attctgtcgc ttccgcggcg gatccgaaaa
      241 tattcttcta cattatacag ttcgtctttt atgaaaacac gataactcag ccggatactg
      301 gcattagaag catcaatttc cagtatttcc atatccttat ccgtaacgac cgaagggttt
      361 aaatcccccc atgtcagctg ctaaaagctg gaatggatgt tgacatagcc atagctggag
      421 ttatctcctt cggaattcga ttccatatac ataacaaggg attttgccgc ttccttatca
      481 aatgtcaggg cagaaaattc ctctacaaac tccatttttt cattcagata gatttccgct
      541 tcatcaataa tacgtgtata ataacggatc tcttttcctg cacctgtcgt cagtttaata
      601 acaagcatat attccgtatt ttcttcaatc agatctttga tcggaatttc cgccgtaatc
      661 acatcgtccg cataagagaa gcctgtcact accgtatctt ctatcaggcg ctgcatatcc
      721 atgcttcgga cctcataggc aaccttgctg atgaccgcac catacgtatc cgcccgaaag
      781 gaaaccgtcc tgtttgcctg aagcggcgtc agtgcgccgc gcagataatt gccttccatt
      841 tccacagtat agccgtgcag acagttcaca tattcgtcat ttacattcat ataaaggaca
      901 ggaagcgttg cagcactcat ttctgcagtc agatcagtat ttccgcggtt aatgattttg
      961 ctgaatacga ttactgaaat taaaaaaaca gctaaaaaaa cgctgagctt gatcaatgcc
     1021 ttcttcataa cttctccaac aattccataa gcctgggcgc agcatgaagc tccttcaaaa
     1081 gcgtttcaag ctcctgcatc tccttctgtc tgggtaattc ccgctgccgt tttactgcgc
     1141 ggatcaaaat atttttcggc gtatgctcca tatcgataaa ttcaagcacc tgcacttcat
     1201 atccctggct ttccaaaagc tctgcccgaa taccgtccgt aaaaagtgcg gacatccgct
     1261 cttttatata cccgtatttc agcaccggtt ccagcagcct gctttctatc tgcccattta
     1321 attcatgctg gcagcaaggt acggacagta taacggaagc accccactgt actgccttgt
     1381 acagcgcata atcagttgcc gtatcacagg cgtgcagcgt tactaccatg tcagccctgt
     1441 ctgcttttgt gtatgttgcg atatctccgc attcaaatga tattttatca taaccgtatt
     1501 tgtcttttaa gaggctgcag gcc
//
LOCUS       DAOSYE010000640         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73872_length_1521_cov_3.154161, whole genome shotgun sequence.
ACCESSION   DAOSYE010000640 DAOSYE010000000
VERSION     DAOSYE010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73872_length_1521_cov_3.154161"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1521)
                     /locus_tag="PLU43_10105"
     CDS             complement(<1..>1521)
                     /locus_tag="PLU43_10105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO28806.1"
                     /translation="AIATSISDIPFYGPCATTQVGMIDGQFIVNPSQEQWKTGDLNLT
                     VASTSEKVIMIEAGANEIPEAKMIDAIYTAHQVNQEIIAFINKMVDEVGKAKHEYTSC
                     AIPEEMFAEMKKIITPEDMETAVFTDEKQVREENIRVITEKLEEAFADNEEWLHVLGE
                     AVYQYQKKTVRKMILKDRKRPDGREITQIRPLAAEIDLVPRVHGSAMFTRGQTQICDI
                     CTLAPLSEKQKVDGLDPNEVTKRYMHHYNFPSYSVGETKPSRGPGRREIGHGSLAERA
                     LIPVLPTEEEFPYAIRCVSETFESNGSTSMASTCASCMSLMAAGVPLKKMVAGISCGL
                     VTGDTDDEYVLLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKIHGLTRPIVEGAIE
                     RCREARLYIMESCMKPAIAEPRKEVGQYAPKIVQIKIDPEKIGDVVGQRGKTINAIID
                     LTGVKIDINDDGSVFICGTEPEGMDKAMELIKIITTDFEAGQIFEGKVVSIKEFGA"
BASE COUNT          371 a          412 c          282 g          456 t
ORIGIN      
        1 aatgctccaa attctttaat gctgaccact tttccttcaa aaatctgccc tgcttcaaaa
       61 tcggttgtaa tgatcttgat cagttccatg gccttatcca ttccctccgg ttccgtaccg
      121 cagataaata ccgaaccgtc atcattaata tcgatcttca ctccagtcag gtcaatgatc
      181 gcattgatcg tctttcctct ctgaccaaca acatcaccga ttttctcagg atcgatcttg
      241 atctgaacaa tcttcggtgc atactgacct acttcttttc taggctctgc aatcgcaggc
      301 ttcatgcagc tttccataat atacagcctt gcttcacggc atctttcaat agcaccttcc
      361 acaataggcc ttgtcaggcc atggatctta atatccatct gaatggccgt aattccttct
      421 gtcgttcctg ttactttaaa gtccatatcg ccaaagaaat cctcaagccc ctgaatatct
      481 gtcaaaagca catactcatc atccgtatcc ccggttacaa gaccgcagga aataccggca
      541 accatctttt tgagcggcac tccggcagcc atcagtgaca tacaggaagc acatgtggaa
      601 gccatggaag tggaaccgtt tgactcaaat gtctccgata cacagcggat cgcatacgga
      661 aattcttctt ccgtcggaag taccggaatc aatgctctct cagcaagtga accatggccg
      721 atctcacggc gtcccggtcc gcgggaaggt tttgtttccc caactgaata cgacggaaaa
      781 ttataatgat gcatatatct ctttgttact tcattcggat caagtccatc caccttctgc
      841 ttctcggaaa gcggtgccaa cgtacaaata tcacagatct gtgtctgtcc gcgtgtaaac
      901 atagcggaac catgtactct tggaaccaga tcgatctctg ccgcaagagg gcggatctgc
      961 gtgatctcac ggccatccgg acgcttccgg tctttcagga tcatctttct cacagttttt
     1021 ttctgatact gataaactgc ttcgccaagt acatgcagcc attcctcgtt atcggcaaat
     1081 gcctcttcaa gcttttcagt gattacacgg atattttctt cacgaacctg cttctcgtct
     1141 gtaaatacgg cagtttccat atcttccggt gtaatgatct ttttcatttc ggcaaacatt
     1201 tcttccggaa tcgcacagct tgtatattca tgctttgcct ttccgacttc gtcgaccatt
     1261 ttattgataa atgcaatgat ctcctgattc acctgatgcg ccgtatagat cgcatcgatc
     1321 attttcgctt ccggaatttc atttgcgcct gcttcgatca tgataacctt ttcacttgtt
     1381 gaagcaactg tcagatttaa atcaccggtt ttccactgtt cctgactcgg attgacgatg
     1441 aactgaccgt cgatcatacc gacctgtgtc gttgcacatg gtccgtaaaa cggaatatct
     1501 gaaatacttg tcgcaatggc c
//
LOCUS       DAOSYE010000641         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_73873_length_1521_cov_3.137108, whole genome shotgun sequence.
ACCESSION   DAOSYE010000641 DAOSYE010000000
VERSION     DAOSYE010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73873_length_1521_cov_3.137108"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="era"
                     /locus_tag="PLU43_10110"
     CDS             complement(<1..324)
                     /gene="era"
                     /locus_tag="PLU43_10110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOO28807.1"
                     /translation="MMTEEKQYKSGFVTLIGRPNVGKSTLMNCLIGQKIAITSNKPQT
                     TRNRIQTVYTSREGQIVFLDTPGIHKAKNRLGDYMVTIANRTLSEVDVVLWLVEASTF
                     IGKGDR"
     gene            complement(321..1331)
                     /locus_tag="PLU43_10115"
     CDS             complement(321..1331)
                     /locus_tag="PLU43_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOO28808.1"
                     /translation="MKKKTAIRFLTGMLLMAAMVIVTGCGSASSDSAAMETSAGYDYS
                     DDIYTKEAAVPATEAVTEEFNDMSDAGSGETETVEVSDDRKLVKTVNMDVETEEFDQL
                     IANVEQKITSLGGYAQNKEIGGRSYYNTDGERYASVTARIPSDKLDDFVTAIAAESNV
                     TSKNESVDDVTLQYVDIEAHRDSLRTEQERLNELLEKADSIETIVALEDRLAEVRYQL
                     ESYESQLRTMDNQVEYSTVYLYISEVERLTPQVEKTPWERISSGFSESLYNVGKGLQN
                     FLIGFIIALPYIFMFAVVIAFIVGVILLSVKKAEKRSKKKMMIRQTKQTKQEPADKAE
                     GK"
BASE COUNT          363 a          398 c          261 g          499 t
ORIGIN      
        1 cctgtctcct ttgcctataa aagtcgaggc ttccacaagc cataaaacaa catccacttc
       61 ggaaagcgtt ctgtttgcaa tcgtcaccat ataatcaccg agacggttct tcgctttatg
      121 aataccgggc gtatccagaa aaacgatctg gccttccctg cttgtataca ctgtctgaat
      181 acgatttctc gtcgtctgcg gtttatttga tgtgattgca attttttgac cgatcaggca
      241 attcatcagc gtggactttc cgacattcgg gcgtccgatc agcgttacaa aaccggattt
      301 atactgtttt tcttccgtca tcattttcct tccgccttgt cggccggttc ctgctttgtc
      361 tgctttgtct gccttatcat cattttcttt ttgcttcttt tttcagcttt ttttacactc
      421 agcaatatga ctccgacaat aaatgcgatc acgaccgcaa acataaaaat gtatggaagt
      481 gcaataataa aaccgatcag aaaattctgc agaccctttc cgacattata gagactttcc
      541 gagaatccgg aactgattct ttcccatggt gttttttcca cctgcggcgt cagacgttcc
      601 acttccgata tatataaata gaccgtactg tattccacct gattatccat tgtccgcagc
      661 tgagattcat aagattccag ctgatatctg acttctgcca ggcggtcttc cagcgcaacg
      721 atcgtctcaa tactatcagc tttttccagc agttcattga gccgttcctg ttccgtacgc
      781 agagaatccc tgtgtgcttc gatatcaaca tactgcagcg taacatcatc cacgctttca
      841 ttcttacttg tcacattgga ttccgcggcg attgccgtca caaaatcatc cagcttgtcg
      901 cttgggatcc gtgcggtaac agatgcataa cgttcaccgt ccgtgttata atagcttctt
      961 ccgccgatct ccttgttctg ggcatatccg ccaagacttg ttattttctg ctccacgttt
     1021 gcgatcaact gatcaaattc ttctgtttcc acatccatgt tgacagtttt aacaagtttg
     1081 cggtcatccg atacttcgac cgtttccgtt tctcccgaac ctgcatccga catatcatta
     1141 aattcttccg tgacggcttc tgtggccggt actgctgcct cttttgtgta aatatcatct
     1201 gaatagtcat aaccagcaga ggtttccatt gctgccgaat cagaagacgc acttccacag
     1261 ccagtaacaa taaccatcgc tgccataagc agcattccag tcaaaaatct gattgctgtt
     1321 ttctttttca tacattttcc tcccataaaa tcaatacttc atcatatgaa ccattcctat
     1381 atcactctgc tattccaaaa acagattttc cactttatca aacaactgtt tctgctgctc
     1441 tattttttct tcacttccca ctacacagat cttgttatcg gaaatgatga ctgtattgtt
     1501 ataggtgtgt cagttgatga t
//
LOCUS       DAOSYE010000642         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74222_length_1517_cov_2.651847, whole genome shotgun sequence.
ACCESSION   DAOSYE010000642 DAOSYE010000000
VERSION     DAOSYE010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74222_length_1517_cov_2.651847"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..853
                     /gene="rpsB"
                     /locus_tag="PLU43_10120"
     CDS             95..853
                     /gene="rpsB"
                     /locus_tag="PLU43_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO28809.1"
                     /translation="MAKANVKDLLDAGVHFGHLTRKWNPKMAPYIYTERNGIYIIDLQ
                     KSVGMVDTAYNAISDIVADGGTILFVGTKKQAQDAIKLEAERCGMFYVNERWLGGMLT
                     NFKTIQSRIKRLKDIERMSEDGTFDVLPKKEVINLKKEWEKLERNLGGIKDMKKIPDA
                     IFVVDPKKERICVQEAHSLGIPLIGIADTNCDPEELDYVIPGNDDAIRAVKLIVSKMA
                     DAVIEANQGQTAEEFVEDFEQAAPQDIEELAEVK"
     gene            856..>1517
                     /gene="tsf"
                     /locus_tag="PLU43_10125"
     CDS             856..>1517
                     /gene="tsf"
                     /locus_tag="PLU43_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOO28810.1"
                     /translation="MAAITAGMVKELRELTGAGMMDCKKALGETDGNIDEAVEFLRKK
                     GLAGAEKKAGRIAAEGIVDVCLVNDDKKAVIVEVNAETDFVAKNEKFQNYVADVAAQA
                     VKSSAKDMDAFMAETWEKDPSLTVKEALSSQISVIGENMNIRRFEQMEEENGFVVSYI
                     HAGGRIGVLIDVETDVINDAVKEMGKNIAMQAAALKPTFTCKSEVDADYIAKEKEILL
                     AQ"
BASE COUNT          509 a          224 c          408 g          376 t
ORIGIN      
        1 ccgagatgtt tagcatccaa acttttaagg cgaatttata tgaccactta aaagttgctt
       61 gatacaatcg gaacgtaaac aaaaaacaaa aaaaatggca aaagcaaacg taaaagacct
      121 attagatgca ggagtgcact ttggtcactt aacaagaaaa tggaatccta aaatggctcc
      181 gtatatttat acggaaagaa acggcattta tattatcgat cttcagaaat cggtagggat
      241 ggttgatacg gcctataatg cgatctctga tattgttgct gatggaggta cgattctttt
      301 tgtaggtacg aaaaagcagg cgcaggatgc aatcaaactg gaggcagagc gctgcggtat
      361 gttttatgta aacgaaagat ggcttggcgg catgcttacc aatttcaaaa cgatccagag
      421 cagaatcaag agattaaagg atattgaaag aatgtctgag gatggaacat ttgatgttct
      481 tccgaaaaaa gaagtcatca atttgaaaaa ggaatgggaa aagcttgaaa ggaatcttgg
      541 cggtatcaag gatatgaaga agattccgga tgcgattttt gttgtagatc cgaaaaagga
      601 gagaatttgt gtacaggaag ctcattcgct gggtattccg ttgatcggta ttgcagatac
      661 aaactgtgat ccggaagaac tggattacgt gattccggga aatgatgatg caatcagggc
      721 cgtaaagctg atcgtgtcaa agatggcaga tgcggtgatc gaggcaaatc agggacagac
      781 agcggaagag tttgttgaag atttcgagca ggcagctccg caggatattg aagaattagc
      841 ggaggtaaaa taataatggc agcaattaca gcaggaatgg taaaagagtt aagagaactg
      901 accggtgcgg gtatgatgga ctgtaaaaaa gcacttggtg aaacagatgg aaatatagat
      961 gaggctgttg aatttttaag aaagaaaggt cttgccggag ctgagaagaa ggcaggcaga
     1021 attgcagcgg aaggtattgt tgacgtatgt cttgtcaatg atgacaaaaa ggctgttatc
     1081 gtagaagtca atgcggaaac ggattttgtt gcaaaaaatg agaaatttca gaattatgtg
     1141 gctgatgtgg cagcacaggc tgttaagtca tctgcgaagg atatggacgc gtttatggca
     1201 gaaacatggg aaaaggatcc gtctcttaca gttaaggagg cactttcttc ccagatctct
     1261 gtgatcggtg aaaatatgaa tatcagaaga tttgagcaga tggaggaaga gaacggattt
     1321 gtcgtttctt atatccatgc aggcggcagg atcggtgtcc tgatcgatgt cgagacagat
     1381 gttatcaatg atgctgtcaa ggaaatgggt aaaaatattg ctatgcaggc agcagcctta
     1441 aagccgacgt ttacatgcaa gagtgaagta gatgcagact atattgcaaa ggaaaaagag
     1501 atccttcttg cgcagat
//
LOCUS       DAOSYE010000643         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74517_length_1513_cov_4.054870, whole genome shotgun sequence.
ACCESSION   DAOSYE010000643 DAOSYE010000000
VERSION     DAOSYE010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74517_length_1513_cov_4.054870"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLU43_10130"
     CDS             <1..651
                     /locus_tag="PLU43_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO28811.1"
                     /translation="VLDHHRQSSERIENATLSYIEPYASSASEMVAEILQYISDDVKI
                     RSVEADCIYSGIMIDTNNFMTKTGVRTFEAAAYLRRNGADVTRVRKLFRDDMVDYKAR
                     AEAVRHAEVYQGVYAVSVSPSDSVESPTIIGAQAANELLNISGIKASFVLTDYQGKIY
                     ISARSIDEVNVQIIMEKLGGGGHMNIAGAQISNVSVEEAKQLLLNTLETMQKEGEL"
     gene            651..1097
                     /gene="rplI"
                     /locus_tag="PLU43_10135"
     CDS             651..1097
                     /gene="rplI"
                     /locus_tag="PLU43_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOO28812.1"
                     /translation="MKVILLEDIKTLGKKGEIVTVSDGYAKNFIFPKKLGAEATKKNL
                     NELKLQKIHDEKMAEKILKEAKEFAEVLETKQVVVKLRTGEGGRTFGSVSSKEIAEEA
                     KLQFDLEIDKKKMQLPEQIKNIGTYEIPVKLHPQVTAMLKVKVEEA"
     gene            1148..>1513
                     /locus_tag="PLU43_10140"
     CDS             1148..>1513
                     /locus_tag="PLU43_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOO28813.1"
                     /translation="MEEALIKRILPHSTEAEQSVIGSMLMDKEAIVIASEILHRDDFY
                     GKQYGILFEAMTELYNTGRPVDLVTLQNHLKEKDVPAEISSMEFIKDLITIVPTSANI
                     KYYANIVSEKAVLRRLIRIN"
BASE COUNT          492 a          257 c          395 g          369 t
ORIGIN      
        1 gttctggatc atcacaggca gagttctgaa cggatcgaaa atgccacact gtcctatatt
       61 gagccgtatg cgtcttctgc gtctgaaatg gtagccgaaa ttctgcagta tatcagtgat
      121 gatgtgaaga tcagatccgt cgaagcagac tgtatttatt ccggaattat gatagataca
      181 aataatttta tgaccaaaac aggagttcgt acatttgagg cggcagcata tttaagacga
      241 aacggcgcag atgtgacaag ggtccggaag ctgtttcggg atgatatggt ggattataag
      301 gcgcgtgcgg aagctgtcag gcatgcggag gtctatcagg gcgtctacgc agtttcggtt
      361 tctccaagcg acagcgtgga aagtccgacg atcatcggtg cacaggctgc caatgaactg
      421 ctgaatatca gcgggatcaa ggcatcgttc gtattaacgg attatcaggg gaaaatttat
      481 atcagtgccc gttcgattga tgaggtgaac gtgcagatca ttatggagaa gcttggcggc
      541 ggcggacata tgaacattgc cggtgcgcag atatcgaatg tatcggttga agaagcaaag
      601 caattactgc tgaatacact ggagactatg caaaaagagg gagaattgta atgaaagtta
      661 ttttgctgga ggacataaaa acattaggga aaaagggaga gatcgtcacg gtcagtgacg
      721 gatatgcaaa aaattttatt tttcctaaaa agcttggggc ggaagcaacc aagaaaaact
      781 taaacgaatt aaaactgcaa aaaatacatg atgaaaaaat ggcagaaaaa atactgaagg
      841 aagcaaaaga gtttgccgaa gtgctggaaa cgaagcaggt agtcgtcaaa ctgcgtacag
      901 gagaaggggg ccggacattt ggttccgtat cttcaaagga aattgctgag gaagcaaaac
      961 ttcaatttga tcttgagatc gataaaaaga aaatgcagct tcctgagcag atcaagaata
     1021 taggcacata tgaaattccg gtaaagctgc acccgcaggt aactgcaatg ttgaaagtca
     1081 aagtagaaga agcataataa cgggcttgta ttttccgtaa tgacattata tcaaggaggc
     1141 cggcttcatg gaagaggcat tgatcaagag aatactgccg catagtacgg aagcggaaca
     1201 gtcggtcatt ggctctatgc ttatggataa agaggcgatc gtcatagctt ctgaaatttt
     1261 gcacagagac gatttttacg ggaaacagta tggaattctg tttgaagcca tgacagagct
     1321 ttataatacc gggagaccgg tggatcttgt taccctgcaa aatcatttga aagaaaaaga
     1381 cgtaccggct gaaatcagca gtatggagtt tattaaagat ttaatcacaa ttgtgccgac
     1441 ttctgcgaat attaagtatt atgccaatat tgtaagtgag aaggcggttt tgcggcgcct
     1501 gatcagaatt aat
//
LOCUS       DAOSYE010000644         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74705_length_1511_cov_3.230769, whole genome shotgun sequence.
ACCESSION   DAOSYE010000644 DAOSYE010000000
VERSION     DAOSYE010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74705_length_1511_cov_3.230769"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLU43_10145"
     CDS             complement(<1..205)
                     /locus_tag="PLU43_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28814.1"
                     /translation="MKKKVLGVLLCAAMTAVMLSACGSSAESAESAEPAKEEAAQEQT
                     EEPAQQAAADDTEEAETTGNKNII"
     gene            complement(273..1253)
                     /locus_tag="PLU43_10150"
     CDS             complement(273..1253)
                     /locus_tag="PLU43_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28815.1"
                     /translation="MNKSSIKAIMAGTGLQKVIAVSALLVLYIFFGIFGNHFLTADTF
                     VSILDSSYYIGFLAIGTTFVIITGGIDLSSGTVMVGSALIGGVAYNVWGFSIGASLLV
                     VMISGLLFGLFNGLLVGRLGLPPFIATLGTQFISLGYCSVIAKVQTQTYPSLTSEDGW
                     FKRVVYKNNGFPVGIIWLILFFAVAWVLLNKTILGKYTYAIGSNEEAVELSGIRSNNW
                     KILVYIVNGFFCGLAGIIYAATYSTITPQTGNGQEMYAIAACVIGGTSLSGGIGSLSG
                     TILGVFVISILKTGLLSMGLPVQWQQVLIGVVVLLAVLVDVVRMKKAKAA"
     gene            complement(1295..>1511)
                     /locus_tag="PLU43_10155"
     CDS             complement(1295..>1511)
                     /locus_tag="PLU43_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528360.1"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HOO28816.1"
                     /translation="DVGARAEIYKLMDELVAQGKSIIMVSSDLTEILRMSDRIIVMCE
                     GRKTGELSIEEANQNNIMTLATKSPAK"
BASE COUNT          403 a          425 c          278 g          405 t
ORIGIN      
        1 cgataatatt tttgttgcct gttgtctccg cttcctctgt gtcatcggct gctgcctgct
       61 gcgccggctc ttctgtctgt tcctgtgccg cctcctcttt tgccggttcc gccgactctg
      121 ccgactccgc ggaactgccg catgcggaca gcatcacagc cgtcatggct gcacacagta
      181 aaactcccaa aaccttcttc ttcatttaaa tttcctcctt catgatatgt cctctatact
      241 aatcctgttt atcaggaaaa gtaactaaat tattatgcgg ctttcgcttt tttcattctg
      301 acaacgtcta caagcaccgc cagcaataca acgaccccga tcagtacctg ctgccactga
      361 accggcagtc ccatcgacag cagtccggtc tttaaaatac tgattacaaa aactccaaga
      421 atcgttcccg acaaagaacc aatccctccg gataaagacg ttccgccgat cacacaggcg
      481 gcgatcgcat acatttcctg tccgtttcct gtctgcggtg tgatcgtact gtatgtggcg
      541 gcataaataa tacctgccag accgcagaaa aatccattta cgatataaac gagaattttc
      601 cagttatttg acctgatacc cgaaagctca acagcctctt cattactgcc gatcgcataa
      661 gtatatttgc ccaggatcgt cttattcaaa agcacccagg caacggcaaa aaacaggatc
      721 agccagatga ttcctaccgg aaatccgttg tttttatata cgacccgttt aaaccagccg
      781 tcctccgatg tcagactcgg atatgtctgt gtctgcacct ttgcgataac agaacaataa
      841 ccaagggaaa tgaactgtgt tcccaatgtg gcaataaaag gcggcagtcc cagtctgccg
      901 acaagcaggc cgttgaacag tccgaacaaa agacccgaaa tcatcactac aagcagcgat
      961 gcaccaatgg aaaatcccca tacgttatat gcaaccccgc cgatcaatgc ggaaccgacc
     1021 atgactgttc cggacgacag gtcgatccct ccggtaataa tgacaaatgt cgtaccaatt
     1081 gccagaaacc ctatataata ggacgaatcc aatatgctga caaaggtatc ggcagtcaga
     1141 aaatgattcc cgaaaatacc gaagaaaata tagagaacca gcaatgcgct taccgcaatc
     1201 accttttgca gtcctgtccc tgccataatt gcctttatgc tacttttatt catgttgttt
     1261 ctgctccttt caaagccctt tattctttat ggcatcattt agccggactt ttcgttgcca
     1321 gcgtcataat gttgttctga tttgcttcct ctatggacaa ttctcctgtt tttctgcctt
     1381 cacacatcac gataattctg tcgctcatac gtaaaatttc cgtcaggtcg gacgacacca
     1441 taataatcga ttttccctgt gcaaccagtt catccatcaa tttatagatc tcagcccgcg
     1501 caccgacatc a
//
LOCUS       DAOSYE010000645         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74730_length_1511_cov_2.610577, whole genome shotgun sequence.
ACCESSION   DAOSYE010000645 DAOSYE010000000
VERSION     DAOSYE010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74730_length_1511_cov_2.610577"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..955
                     /locus_tag="PLU43_10160"
                     /pseudo
     CDS             218..955
                     /locus_tag="PLU43_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            1005..>1511
                     /locus_tag="PLU43_10165"
     CDS             1005..>1511
                     /locus_tag="PLU43_10165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO28817.1"
                     /translation="MSIKRVYAEIDLSAIQSNMEHMNAMIDKHTKIIAVIKADGYGHG
                     ALQIAQKFECSEYVFGYATATVEEAVDLRENNIKKPILILGYTFPEDYESIIKYEISP
                     AVFKAETARQLSACAEKMHKKVHVHIKVETGMSRIGVSPDDRGLSVVKEIAACPWIEV
                     EGMFTHFAR"
BASE COUNT          461 a          269 c          410 g          371 t
ORIGIN      
        1 gttcgcattt aagaaaacag gccaattgat ttatccgggt tttattcata caggggagct
       61 gatcaaaaaa gagatcggta tcacagaggc atcgcttggc ggagaaattc ccctgatcag
      121 tgcattacag aaaaaagatc ttatcatgaa aaagagaccg gcatattcca ataaaggaac
      181 attcggcaaa gtattgctga ttgcaggcag caaggcaatg gccggtgcgg ctgttcttgc
      241 agccagggcc tgtctgaaaa gcggaaccgg tatggtacag gtgtatacgc atgaaaataa
      301 cagggctgtt ttgattgaga gcctgccgga agcgatcgta acgacttatt ccgcagacgc
      361 agagcttttt taactggaaa cagcgataaa atgggcggat ataattggta ttggtcctgg
      421 catttcactg gaaagacagg ctgaggagct cctgcgtgtt gttctggagt gcagtgaaaa
      481 accgcttgtt atagatgcgg atgcgattac tcttttagac gatacaaaaa agcagcagct
      541 gaaagaacag gcagatcgtc cgattattat gacgccgcat atcggagagt tctgccgatt
      601 tacaaagatg aaaaaggaag ctgctctgac tgatgtgatt ggatcagcaa gaaaagcggc
      661 acaggaatat catttggttt gtgtatgtaa ggatgcgaga acagtaatcg cagatccgtc
      721 tggtttatgc cggatcaata cgaacggaaa cagcggaatg gcaagtgcag ggaccgggga
      781 tgctttattt ggaattatct gcggactctt ggcacagggg ctcctgccgt ttgaagcggc
      841 gtcgttcggg gtgtatctgc atggggaggc cggcgatctt gcggctgcaa aatgcgggga
      901 acatgggatg cttgctacgg atctgatcga ttctttacgg gaagtgctgg actgactgac
      961 acaaaaaact gataggaatg tctgaaaata aaggagaaat ggaaatgagt ataaaaaggg
     1021 tttatgctga aattgattta agtgcgattc agtctaatat ggagcatatg aatgcaatga
     1081 tcgataaaca tacgaaaatc attgctgtga tcaaggctga cggatatgga catggagcac
     1141 ttcagatcgc gcagaaattc gagtgctcag aatatgtttt cgggtatgca acagcgactg
     1201 tggaggaagc tgtcgaccta agagagaata atattaaaaa accgatcctg attttaggat
     1261 atacgtttcc ggaagattat gaatcaatta taaaatatga aatcagtcct gccgtattta
     1321 aggcggaaac agccagacag ctttccgcct gtgcagagaa gatgcataaa aaagtacatg
     1381 tacacatcaa agtcgaaacc ggaatgtcaa ggatcggcgt atctcctgat gacaggggat
     1441 tgtctgttgt aaaagagatc gcagcctgtc catggattga agtagaaggt atgtttactc
     1501 attttgcccg t
//
LOCUS       DAOSYE010000646         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74882_length_1509_cov_3.347318, whole genome shotgun sequence.
ACCESSION   DAOSYE010000646 DAOSYE010000000
VERSION     DAOSYE010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74882_length_1509_cov_3.347318"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLU43_10170"
     CDS             <1..326
                     /locus_tag="PLU43_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28818.1"
                     /translation="DRILFSGDGIIEQIWMQLPESLKIADLIRSMNRLKYLRPEYDHI
                     LTGHSRGLEDASLFNDLLAAAIDLENGNTKEDVSYEWFDGTCMAHPYGKEPHKIVYDP
                     AFSLI"
     gene            complement(414..>1509)
                     /locus_tag="PLU43_10175"
     CDS             complement(414..>1509)
                     /locus_tag="PLU43_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO28819.1"
                     /translation="ILEYESLSQSSQYYYDEDFIISTLVFAKDDPQIIYPSIKKLIEL
                     GAAGMAVKSAYFKSLPKDVLQLANNKMFPIFMFEDLYIEEVIISITEYIRERQQIGIF
                     ERQISRLLYGKTDVFLTEQYAVEMNPGRREAACCLFMQVKDSKLHWIHKIKDALLMRS
                     NRAIADQYRFLQYKRGFFVISNLYEPESQKQIKEDFSSVFRRLGCSLDGIHAGISTVK
                     ESIAEFDCMLQEALDAYLYGNLFDRNVSIFDEIGIYRFLFPIYRDKGSRRIFDGMKQA
                     ICEYDTESSGDLQETLLMYLKENQDIRKTAEKMNQHANTIRYRLGKIAELTGCHTGVA
                     EKNQLFLLGEYIRFEHCMKNSACFDRQDAP"
BASE COUNT          408 a          360 c          280 g          461 t
ORIGIN      
        1 ccgaccgcat cctgttcagc ggtgacggca ttatcgaaca gatatggatg cagcttccgg
       61 aatcgctgaa aatcgccgat ctgatacgca gtatgaatcg tcttaaatat ctgcggccgg
      121 aatacgacca tattttgacc ggacacagca gaggcttaga agatgcttct ttgttcaatg
      181 acttgctggc tgcagcaatt gatttggaaa atggaaatac gaaggaggat gtctcctatg
      241 aatggtttga cggtacatgc atggcacatc cttatggaaa agagccgcac aagatcgtat
      301 acgaccctgc tttttctcta atttaatatt gggtctatcg tcacataaat aaagcagaat
      361 caataaagat cgattctgct ttttccgtat cacatggcgg ttttttacaa ttttcatggt
      421 gcatcctgcc tgtcaaaaca tgcggaattt ttcatacaat gttcaaaacg aatatattcc
      481 cctaaaagaa acagctggtt tttttccgca actcccgtat gacagccggt cagctctgcg
      541 atcttaccaa gccggtaacg gatcgtattc gcatgctggt tcattttttc cgctgttttt
      601 ctgatatcct gattttcctt aagatacatc agcagggtct cctgcagatc cccgctgctt
      661 tctgtgtcat actcacaaat tgcctgcttc attccgtcaa atatccgtct gcttcctttg
      721 tctctgtata tcggaaacag aaaccgatag ataccgatct cgtcaaaaat actgacattc
      781 ctatcaaata aattcccgta taaataggca tccagggctt cctgcagcat acagtcaaac
      841 tctgcgatac tttctttaac agtactgatc cctgcatgaa ttccatcaag agaacaccct
      901 aatcgccgaa atacggaaga aaaatcttct tttatctgtt tctggctttc aggctcatat
      961 aaattgctga tcacaaaaaa gcctcttttg tactgcaaaa aacgatattg atccgctatg
     1021 gctctgttgc ttctcatcag caatgcatct ttgattttat ggatccaatg cagtttgctg
     1081 tcttttacct gcataaacag acagcaggcg gcctccctcc tgccaggatt catttccact
     1141 gcgtactgtt ccgtcaaaaa aacatctgtt tttccataca gcagtctgga gatctgtctc
     1201 tcgaatatac cgatctgctg cctttccctg atatattccg taatgcttat gatcacttct
     1261 tcgatataca gatcctcaaa cataaaaatc ggaaacatct tattattggc cagctgcagc
     1321 acgtcctttg gcagcgactt aaagtaagcg gactttacgg ccatccctgc tgctcccagc
     1381 tcgatcagtt ttttgatgga tggataaata atctgaggat cgtcctttgc aaatacaagc
     1441 gtgctgatta tgaaatcttc atcataataa tactgactgc tttgactgag gctttcgtac
     1501 tcaagaatt
//
LOCUS       DAOSYE010000647         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74886_length_1509_cov_3.143741, whole genome shotgun sequence.
ACCESSION   DAOSYE010000647 DAOSYE010000000
VERSION     DAOSYE010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74886_length_1509_cov_3.143741"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..950)
                     /locus_tag="PLU43_10180"
     CDS             complement(66..950)
                     /locus_tag="PLU43_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28820.1"
                     /translation="MPRNKEDIVEDEEQEETDKKGKGRKKRDKKKKGDQQESFDATVT
                     NEGAMYTDAESDEEGGSASVVLIVIFIVFIWLAILGLLIKLDIGGFGSNVLYPVLKDV
                     PVVNMILPSGVADEAAADESDYKTLADAVAEINRLNEEIDQLKSEQENAGSTSDEEVA
                     SLKEEIKRLQTFEDSQVEFQKIKTEFYEEVVFSDNAPDIEEYKAYYESIDPENAEYIY
                     KQVIQQIETNQEMDEYVKAYSEMKPKQAAAIFEMMTDNLDLVAKILGEMDTDSRANIL
                     GVMDSQVASQITKIMDPE"
     gene            complement(956..1405)
                     /gene="fliJ"
                     /locus_tag="PLU43_10185"
     CDS             complement(956..1405)
                     /gene="fliJ"
                     /locus_tag="PLU43_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOO28821.1"
                     /translation="MSKFIYRMQNLLNIKIELEAQAKNEFAIANARLRAQEKILNALN
                     LQKDGYEEELKKLYSKNLKIREITETQQAVEVMKYRIRLQILQVKHAQKNVETARLKL
                     QEVMQERKKHEKLKEKKFEQFLLDEAADERKAIDELVSYRHGHKKEE"
BASE COUNT          345 a          407 c          236 g          521 t
ORIGIN      
        1 tgctatcaaa tattttatcg gccgttttcc atgaaaactt aagaaaaacc gatcactttt
       61 tatgactatt ccggatccat gatctttgtg atctgtgaag cgacctgtga atccataact
      121 cccagaatat tagccctgct gtccgtatcc atctcaccca gaattttggc aaccagatcc
      181 agattatccg tcatcatctc aaagatcgca gcagcctgtt tcggtttcat ctcgctgtat
      241 gcctttacat actcgtccat ttcctgattg gtctctatct gctggatcac ctgtttataa
      301 atatattccg cattttccgg atcaatactt tcatagtaag ccttatattc ttcaatatcc
      361 ggtgcattat ccgaaaaaac gacctcttca taaaactctg tcttaatttt ctgaaattct
      421 acctggctgt cttcaaaagt ctgcagcctc ttgatctctt ccttgagtga cgccacctct
      481 tcgtcgcttg tggaaccggc gttttcctgc tcgcttttca gctgatcgat ctcctcattc
      541 agcctgttga tctctgccac cgcatctgcc agtgttttat aatcactttc atcagcagca
      601 gcctcatcag caactcctga cggcaggatc atattgacga caggaacatc ctttaaaaca
      661 ggatagagca cattggaacc aaacccgccg atatccagct tgatcagcag accaagtatg
      721 gccagccaga tgaatacaat aaaaataacg atcagcacaa cggaagcaga accgccttcc
      781 tcatcgcttt ccgcgtccgt atacatcgct ccttcatttg ttacggtcgc atcaaagctt
      841 tcctgctgat ctcccttttt ctttttatcc cttttctttc gtccttttcc ttttttatca
      901 gtttcttcct gctcttcgtc ttcgacgatg tcttctttat ttcttggcat cagtctcact
      961 cctctttctt atgtccatgt ctgtaactga caagctcatc aattgccttg cgctcatccg
     1021 ccgcttcgtc aagcaggaac tgttcaaatt tcttttcctt cagcttctca tgcttcttcc
     1081 gctcctgcat aacctcctgt aacttcagcc gtgccgtctc cacgttcttc tgcgcatgtt
     1141 ttacctgcag tatctgaagc ctgattctgt atttcatcac ttcgaccgcc tgctgtgttt
     1201 ctgtaatttc cctgattttt aaatttttag aatataattt tttcagttct tcttcataac
     1261 cgtctttttg caggttaagg gcattcagaa ttttctcctg tgcgcgaagc ctggcattcg
     1321 caatcgcaaa ttcatttttg gcctgcgcct caagttcgat cttaatatta aggagattct
     1381 gcatccggta aataaatttt gacataagca atttcccctt tatctgactg ccgcaaaata
     1441 atttatgtaa atatcgaatc cagcatctga acctcttctt caaatgtata ttttgcttct
     1501 acatcctga
//
LOCUS       DAOSYE010000648         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74905_length_1509_cov_2.546768, whole genome shotgun sequence.
ACCESSION   DAOSYE010000648 DAOSYE010000000
VERSION     DAOSYE010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74905_length_1509_cov_2.546768"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..67)
                     /locus_tag="PLU43_10190"
     CDS             complement(<1..67)
                     /locus_tag="PLU43_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO28822.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRR"
     gene            complement(295..>1509)
                     /locus_tag="PLU43_10195"
     CDS             complement(295..>1509)
                     /locus_tag="PLU43_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO28823.1"
                     /translation="LKGNVQIVVGTPGRVMDHMRRHTLKMEQLTTIVLDEADEMLNMG
                     FREDIETILCDIPQEHQTVLFSATMPKEILQITNQYQKEDAVNVKIQAKELTIPLVKQ
                     YYYEVNRQNKEEVTARLLDYYNPKRSLIFCNTKRMVDELAENLTGRGYFAEGLHGDLS
                     QAQRDRVMAGFRNGTTEILIATDVAARGIDVDNVEAVFNYDVPQDIEYYVHRIGRTGR
                     AGKKGRSFTLVSGRDIYKIREIERTCNTKIKVRTVPSAADITAAKSEKILRGVIEALH
                     GKDLSVMMRIIEQTLDEEDCTAIEIAAAFLKQQMGEEIQELQLDEPYRKRRGPRYESA
                     DKKGRGKSYGADKATDRGHHKKPGEYVKQKTGKKKHTYKDDREKSDKVKTVKAKDINR
                     KGEIRKHKSEFI"
BASE COUNT          317 a          415 c          272 g          505 t
ORIGIN      
        1 atcttcttgt ctgatgtccg aaatgaacac ctgcttccag taactgtttc attgaaataa
       61 cgctcatgtt tctacctcca tttggttaag tacctccgca tgcttctgct ttacgggtta
      121 ttccgctgat atccggaaca ccatcccaca aatcggcaca cgtgattatt ttcaggtctg
      181 ctcctgcaaa cccagtcaac ctatgcatta tatcatagga aaaaatgcgt ttcaagcctt
      241 ttatacgtat ttcctgaaat tttttacaac caacagtccg gtattgatct aaaatcagat
      301 aaactcactt ttatgctttc tgatctcacc ttttcgattg atatcttttg ccttgactgt
      361 ttttacttta tcgctctttt ccctgtcatc tttatatgta tgtttctttt ttcccgtttt
      421 ctgttttacg tattccccgg gttttttatg atgtccgcgg tcagttgcct tatcggcacc
      481 atagcttttt cccctgcctt ttttatctgc gctctcatat cgcggacccc tgcgcttccg
      541 gtaaggctca tccagctgaa gctcctgtat ttcttctccc atctgctgct ttaaaaacgc
      601 tgcggcgatc tctatcgcag tgcagtcttc ctcatccagc gtctgctcga tgattctcat
      661 catgacactc agatcttttc catgtaaagc ttcaatgacc ccgcgcagaa tcttttccga
      721 ctttgccgcc gtaatatccg ccgcactcgg cacagtacgc actttgatct ttgtattgca
      781 ggtgcgctcg atttccctga tcttataaat atcccggcct gatacaagtg taaaggaacg
      841 tccctttttt ccggccctgc cggtacgtcc gatccggtgc acataatatt cgatatcctg
      901 cggtacgtca taattaaaga ctgcttccac attatccaca tcgattcctc ttgccgccac
      961 atccgtagca atcaggatct ccgtcgtgcc gttcctaaag ccggccataa cacggtctct
     1021 ctgtgcctgt gacagatctc cgtgaagccc ttctgcaaaa tatcctcttc cggtcagatt
     1081 ttccgccagt tcgtctacca ttcgttttgt attacaaaag atcagactgc gtttcgggtt
     1141 ataatagtcc agaagtcttg ccgttacctc ttctttattt tgacggttta cttcataata
     1201 atactgcttt acaagaggga tcgtcagttc ctttgcctgg atctttacat tgactgcatc
     1261 ttccttctga tactgattcg tgatctgcag aatctccttt ggcattgttg cggaaaacag
     1321 cacggtctgg tgttcctgcg gaatatcaca caggatcgtt tcaatgtctt cgcgaaagcc
     1381 catattcagc atttcatccg cttcgtcgag cacgatcgtc gtaagctgct ccatctttaa
     1441 tgtatgacgg cgcatatggt ccatgacccg gcccggcgtt ccgacaacga tctgcacatt
     1501 tcctttcag
//
LOCUS       DAOSYE010000649         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74919_length_1509_cov_1.974553, whole genome shotgun sequence.
ACCESSION   DAOSYE010000649 DAOSYE010000000
VERSION     DAOSYE010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74919_length_1509_cov_1.974553"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLU43_10200"
     CDS             complement(<1..273)
                     /locus_tag="PLU43_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOO28824.1"
                     /translation="MKKLLCKADLEAVRAEASKEIRAQECRILICAGTGCLAGGSLNI
                     YKKMCELTKDDETTQIEFGEEIAHGPEVHASKVGVKKSGCHGFCEMG"
     gene            complement(270..749)
                     /locus_tag="PLU43_10205"
     CDS             complement(270..749)
                     /locus_tag="PLU43_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO28825.1"
                     /translation="MLEQFYYDKADEIIAEHGKEQKALIPIMQDIQNEYRYLPPELLT
                     YVAQQIGITEAKAFSVATFYENFSFEKKGKYVIKVCDGTACHVRKSTLILEKLYQELG
                     LSRDKHTTDDMKFTLETVSCLGACGLAPVLTVNDKVYPSMNPEKVTALLEKLRREEE"
     gene            1033..>1509
                     /locus_tag="PLU43_10210"
     CDS             1033..>1509
                     /locus_tag="PLU43_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO28826.1"
                     /translation="MNYRNNNGNNDLSALGFGCMRFPKRGNGFDMEEVEKEILHAIKL
                     GVNYFDTAYIYPGSEEALGTVLSVNGCREQVKIATTLPHYMMKTRADMEKHFNEELSR
                     LKTDYIDYYLMHMLPDVKTWNILIERGALDWLTELKASGKIRNIGFSYHGSTHTFQA"
BASE COUNT          430 a          353 c          263 g          463 t
ORIGIN      
        1 tcccatttca caaaatccgt gacatccgct ttttttgaca ccaacttttg aagcatgaac
       61 ttccggacca tgtgcgatct cttccccaaa ctcgatctgg gtagtttcat cgtctttcgt
      121 aagttcacac atttttttat atatattcag cgagccgccc gcaaggcagc ctgtaccggc
      181 acaaataagg atcctgcact cctgtgcacg gatctctttt gacgcttctg ctcttacggc
      241 ttccaaatcc gctttgcata ataacttttt cattcctctt ccctccgcaa cttttcaagt
      301 aatgccgtta ccttttccgg gttcatcgaa ggatatacct tgtcattcac cgtcagcacc
      361 ggtgcaagac cgcatgcgcc aagacaggaa actgtttcca atgtaaattt catatcgtcc
      421 gtcgtatgct tgtctctgga aagacccagt tcctgataca gtttttctaa aatcagcgtg
      481 gatttacgaa catgacatgc cgtaccgtca catactttaa ttacatactt tccctttttt
      541 tcaaacgaaa aattctcata aaatgtcgca acactgaagg ctttcgcctc cgtaattcct
      601 atctgctgtg ccacataagt caaaagctca ggcggcagat aacggtattc attctgaata
      661 tcctgcatga tcggaatcag tgctttctgc tcttttccat gctctgcaat gatttcatct
      721 gccttgtcat aataaaactg ctccagcata ttttttctcc ttatcagtta ctttatcagt
      781 tactttttgt cacttgttta accattatac ataatttgtg tttcattctc aagctatttg
      841 ataaaaattt atcaatcttt tatattatac acattttgct tacaaaaaca ttaaaaaaac
      901 tgttaaaaat catcaattga aaatttctta tacgcttgct atactgggat tatatacaaa
      961 aaattctgtc aattatccgg aaaggagcat gcgctgttat ccaatcatat aagtcagcgc
     1021 ttatcatcga ttatgaatta tagaaataat aacggaaata atgacctgtc ggctttaggt
     1081 tttggatgca tgcgctttcc aaaacgagga aacggctttg acatggaaga ggtcgagaaa
     1141 gaaattctgc atgcgatcaa gcttggtgtc aactatttcg acacagccta tatctatcct
     1201 ggcagtgaag aagcgctcgg caccgtcctt tccgtaaacg gctgcaggga acaggtcaaa
     1261 attgcaacaa cgctgcctca ttatatgatg aaaacacggg cagatatgga aaaacacttt
     1321 aacgaagagc tctcccggct gaaaacagac tatatcgatt attatttgat gcacatgctg
     1381 ccggatgtga aaacatggaa tatcctgatc gaacgcggag cacttgactg gctcaccgaa
     1441 ctgaaagcat ccggtaaaat cagaaatatc ggtttttcct atcatggcag cacacatacg
     1501 tttcaggcc
//
LOCUS       DAOSYE010000650         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74959_length_1508_cov_3.683414, whole genome shotgun sequence.
ACCESSION   DAOSYE010000650 DAOSYE010000000
VERSION     DAOSYE010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74959_length_1508_cov_3.683414"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLU43_10215"
     CDS             complement(<1..481)
                     /locus_tag="PLU43_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28827.1"
                     /translation="MKGFRKVMVAFGVVLCAGIVITFSVPAKAATAEDLANQARWQAD
                     QEAQAALYASQVAAYNAENAKNQARWEADQKALAAENAKNQARWQADQEAQAALYASQ
                     VAAYNAENAKNQARWQADQEAQAALYASQVAAYNAENAKNQARWQADQEAQAALYASQ
                     "
     gene            complement(638..1087)
                     /locus_tag="PLU43_10220"
     CDS             complement(638..1087)
                     /locus_tag="PLU43_10220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO28828.1"
                     /translation="MEFRQIRKDDLKQFLEMRMEFAALMREIPDMKSFRDVTETYLEE
                     HIGKDDLVVVLAVDSGQIISSCMACIFQTAPLPSCPSGKIAELLNVYTKKDHRGNGYV
                     TKLIRMLMEELKSRGVGKIILSYTDAGYPVYKKLGFTEENQEMSLTL"
     gene            complement(1139..>1508)
                     /locus_tag="PLU43_10225"
     CDS             complement(1139..>1508)
                     /locus_tag="PLU43_10225"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOO28829.1"
                     /translation="LVKANRTVVYNSNISMEKLVTEHIISGPLSENVYPTVSPEIKII
                     SVTVKDGTCYVNLDQSFLTKSQKVSDEVVIYSFVNSLTELPNVNKVQFMIDGESEITF
                     GDHIYLSDPFERNLEIVSQQ"
BASE COUNT          371 a          393 c          251 g          493 t
ORIGIN      
        1 cctggctcgc gtacagggct gcctgtgctt cctgatctgc ctgccatctt gcctgattct
       61 ttgcattctc cgcattatat gctgctacct ggcttgcgta cagggctgcc tgtgcttcct
      121 gatctgcctg ccatcttgcc tggttcttcg cattttcggc attgtacgct gctacctggc
      181 tcgcgtacag ggctgcctgt gcttcctgat ctgcctgcca tcttgcctgg ttctttgcat
      241 tttcggctgc cagtgccttc tgatctgctt cccatcttgc ctgattcttt gcattctctg
      301 cattgtacgc cgctacctgg cttgcataca gcgctgcctg cgcttcctga tctgcctgcc
      361 atcttgcctg attagctaaa tcttctgcag ttgctgcctt tgccggtaca gaaaaagtaa
      421 taacaatccc cgcgcaaagt acaaccccaa atgccaccat tacttttcta aaacctttca
      481 ttacatcgtc acctcctgat ttattgacaa tagttacaaa ataaatatgt aaatacatta
      541 tattacaatt ttcctaatta ttcaatatcc aaattattac tttacaaaaa atagacaaaa
      601 aaaatacacc tgcaatgctc ttttttacat ccgtcgctta caacgtaagc gacatctcct
      661 gattttcctc tgtaaaaccc agttttttat atacaggata gcctgcatcc gtataagaca
      721 agataatctt gcccactccg cggcttttta attcttccat cagcatacgg atcagcttcg
      781 tcacgtaacc atttcccctg tgatcctttt ttgtataaac attcagcagt tccgctattt
      841 ttccggacgg acaggacggc agcggtgctg tctgaaaaat acaggccatg caggaggata
      901 tgatttgacc gctgtctacg gcaagaacga ctaccagatc atcctttcca atatgttctt
      961 ccaaataggt ttccgtaaca tccctaaaac ttttcatatc cgggatttct ctcatcaagg
     1021 ccgcaaactc cattctcatt tctaaaaact gcttaagatc gtccttcctg atctgtctga
     1081 attccatctt tactcctcct gtctttttct gaaaattctt tttaaacata ccgttttatt
     1141 actgctgact gacgatttcc agattgcgtt caaacggatc cgataaataa atgtgatcac
     1201 caaaggtaat ttctgattca ccgtctatca taaactgaac cttgttaaca ttcggaagct
     1261 ctgtcagtga atttacaaaa gaatatatca cgacttcatc cgacactttc tgagattttg
     1321 tcaaaaatga ctggtccagg tttacataac aagtcccgtc ttttaccgta acactgatta
     1381 tttttatttc cggtgaaaca gtcggataca cgttttcaga cagcggtccg gatataatat
     1441 gctctgtcac gagcttttcc atggaaatat tactgttata taccacagtc cgattggctt
     1501 ttaccaga
//
LOCUS       DAOSYE010000651         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_74971_length_1508_cov_3.022712, whole genome shotgun sequence.
ACCESSION   DAOSYE010000651 DAOSYE010000000
VERSION     DAOSYE010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74971_length_1508_cov_3.022712"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..>1508)
                     /locus_tag="PLU43_10230"
     CDS             complement(199..>1508)
                     /locus_tag="PLU43_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO28830.1"
                     /translation="EASDYLVSLLEKHGFKIDRGMSDMKTSFIAVYGTGSPVIGYNAE
                     YDCLPGLSQQCCQDKKEPVIEGAPGHGCGHNILGTAEVFAAISLKRFMEEANIEGTIK
                     VFGSPAEELCVGKPFMARDGYYKEVDCFLDWHPWSYNKAHYDTCGAYFNVKYHFKGKT
                     CHGNSPWQGRSAFDAAMLTGTAIEFLREHYEPAAADRANTINYTFSETGPDFPSVVPD
                     RTTLWCIGRFNTSKQLKEIMARVDNCAKAGALATGTEMEKEFITASHEKIPNKVLAKV
                     VYHNFKEIGAPLFSQEEQALAKRMQKEDGYEETGLDTVLQDFGTSGTVLCDTSEFSWH
                     APYATFWMTAAPDGGWHNWKVTSCVGSSIGMKAMRQAAKVLAVSGLDLLRDPDIVNAA
                     KKEFEERMEGMRYETLLPPNAKVPNGINFAAMQKYRASDRRKV"
BASE COUNT          377 a          406 c          263 g          462 t
ORIGIN      
        1 tcgcttcgtc cttagataac gaaaatgaaa agctgtcctt tttcatgcat tccgcaatag
       61 agaccatctc ttcctttatc gcatgtgaaa ttcttagtgc cgcatatttg ctgtttcctg
      121 tcgccgaaaa atatagtatc ataaaagccc tcctttccat tgaccacgac agtacctata
      181 caaatattat atctgaattt acacttttcg tctatcagaa gctctgtatt tctgcattgc
      241 tgcaaaattg attccatttg ggacctttgc atttggcggt aataatgttt cataacgcat
      301 cccttccatg cgttcctcaa attctttttt tgcagcattg acaatatcag gatcccttaa
      361 cagatccagg ccggaaacag caagtacttt tgctgcctgt ctcatcgctt tcataccaat
      421 actgcttccc acacaggaag tcactttcca attatgccac ccgccgtccg gcgctgcagt
      481 catccaaaag gtagcatacg gcgcatgcca gctgaattcc gacgtatcac aaagcaccgt
      541 accgcttgtt ccaaaatcct gcaaaaccgt atccagtccg gtttcctcat atccgtcttc
      601 cttttgcatc cgtttagcca gtgcctgctc ttcctgtgaa aacagcggtg caccgatttc
      661 cttgaaatta tgataaacga cctttgccaa tactttattc ggtatctttt catgacttgc
      721 cgtgataaat tccttttcca tttcagttcc tgttgcaagg gctcctgcct ttgcgcaatt
      781 atcaacacgc gccatgatct cttttaactg ctttgacgta ttgaatcttc cgatgcacca
      841 taaagtcgtc cgatccggca caacgctcgg aaagtccggg cctgtttccg aaaaggtata
      901 attgatcgta ttagcacggt ctgccgccgc aggttcataa tgttcacgta aaaattcgat
      961 cgccgtaccg gtcagcattg ccgcatcaaa tgcactcctg ccctgccatg gagaattccc
     1021 atggcatgtt tttcccttga aatgatattt tacattaaaa taagcaccgc atgtatcata
     1081 atgtgcttta ttatagctcc atggatgcca gtctaaaaaa caatctacct ctttataata
     1141 tccgtctctt gccataaatg gcttgcctac gcacaattcc tctgccggtg agccgaaaac
     1201 ctttatcgtt ccctctatgt ttgcctcttc cataaacctt ttcaaagaga ttgcggcaaa
     1261 gacttccgct gttcctaata tattatgacc gcatccatgg ccgggcgccc cttcgataac
     1321 aggctctttt ttgtcctgac agcactgctg tgacagccct ggaaggcagt catattccgc
     1381 attatatcct attaccggag agcctgttcc ataaactgcg ataaacgaag ttttcatatc
     1441 cgacattccg cggtctattt taaatccatg cttctccagc agggaaacaa gataatccga
     1501 tgcttcat
//
LOCUS       DAOSYE010000652         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75230_length_1505_cov_2.313103, whole genome shotgun sequence.
ACCESSION   DAOSYE010000652 DAOSYE010000000
VERSION     DAOSYE010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75230_length_1505_cov_2.313103"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1130)
                     /locus_tag="PLU43_10235"
                     /pseudo
     CDS             complement(222..1130)
                     /locus_tag="PLU43_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
     gene            complement(1184..>1505)
                     /locus_tag="PLU43_10240"
     CDS             complement(1184..>1505)
                     /locus_tag="PLU43_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28831.1"
                     /translation="IKEFDMPQILHIDRLCNECGNCASFCPYTGEPYRDKLTLFASDA
                     DFENSDNQGFLFIDKEKPVIKVRTGGRIITADGLPALTQQGAESGLAAFIRTVYEEYG
                     YLYR"
BASE COUNT          358 a          436 c          299 g          412 t
ORIGIN      
        1 aaagccatcg gcaccagcag cgccaatacc acaccgacct tcaccgtggt gtttcgtcgc
       61 gtgaacagcg caaacatcgg gatgatgggc aaaccaacca tacctggcgc cgcgatgccc
      121 ccggaatacc atccgcagat aaccagcaaa gccagcaatg cgtacatgcc tatgtagtca
      181 ctgtaacggc tgagcaatct tgaacgcgtg gacaatggcg cggccatcag cagcggcagc
      241 accaggtcaa agatcgtccg ctttgcatac atgacgcagc ccggaagacc catgatcggg
      301 atcccgtttt cgttatatgc aagcaagaac atcgcccccg gcaaaacagg tgcgccgtag
      361 cttacaatat gcgcacctgt attttttatc gcaagcggcg tcctgtcatc tggatccaca
      421 ctcattcctc cggtacagac gaccatatcc gcaccctgct gcaaaaattc attgattgct
      481 cctgtaatct tttcgtgatc atccgaacaa agcctctgtc cgatgatacg agctccatat
      541 tcagtcagtt tatcgatcac gaccggcgta aaggtatctt ctatccggtg cttatatact
      601 tcgcttcctg tcgtaacgat cgccgctttc tttttatgaa acggcctgac ttcaaagatt
      661 cctttatacg gcttcgccgc ctccattttt tctttttgaa tgatcagggg aattacgcgc
      721 atgcctgcca gcttatcacc ttttttcacc ggtgtatccc catgccggga ggctatcatc
      781 atctctccga gactgtttac tgcaaccaga tgttttcggt tgattttcag cagtccgtca
      841 cagtctgcgt aaagctcgat ctttccttcc tttggctgcg ttgcatgcat gttggcacca
      901 atagaaatat ctcgcagcca ttctgcagct tcattttcat gcagcattcc ctctgtccgt
      961 tcccacacgt acagattttc cttgcccacc gaaagcagta cgggaatgtc ctcctttgtc
     1021 acgatatgtc ctttccgaaa gaccgcatcc tttgatacat ccttgatgat cctcgtaata
     1081 tcatgacaaa gcacatgacc gaccgcatct tcagttttta ttaatttcaa accatgtcac
     1141 cccgctgtta ttttaatttc agtaatcccg tcgttattta caattatctg tacaaatatc
     1201 cgtattcctc atatacggtt cgtataaatg cagcaagccc gctctctgca ccctgctgcg
     1261 tcaaagcagg cagaccatct gccgtaatga tccttccgcc ggtacgcacc tttatgacag
     1321 gcttctcttt atcaataaac agaaagccct gattatcgct gttttcaaaa tcggcatccg
     1381 aagcaaacaa ggtcagctta tctctgtacg gctctcccgt atacggacag aagcttgcgc
     1441 agttcccgca ctcgttgcac aagcggtcga tatgcagtat ctgcggcatg tcaaactctt
     1501 ttatt
//
LOCUS       DAOSYE010000653         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75457_length_1502_cov_2.626814, whole genome shotgun sequence.
ACCESSION   DAOSYE010000653 DAOSYE010000000
VERSION     DAOSYE010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75457_length_1502_cov_2.626814"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..>1502)
                     /locus_tag="PLU43_10245"
     CDS             complement(334..>1502)
                     /locus_tag="PLU43_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO28832.1"
                     /translation="VIPGHNITLSALGVFILWFGWFGFNGCSTLRFEGDAIELASKIF
                     VNTNMAAAVATCTSMITSAIRYKKPDISMTLNGALAGLVAITAGCDTVSLTGAFFIGL
                     CAGIVVVFSVEFIDKILKVDDPVGAVGVHGVCGALGTILVGLLSTENGLLYTGKGFFF
                     LTQIIGVISVITWVSAIMFLVFSVLKSTIGLRVKEQDEVEGMEKSEHGLLNAYADFLP
                     VAAINSYGGKYNYYKAAQDSNGEDIEDITEKQTIYESYESAADFERDEQNAHITKVEI
                     ILKQNKFEELKAAMNDIGVTGMTVTQILGCGMQKGATEFYRGTEFEMQLLPKVRVEMV
                     IAKVPVKKVVETARRVLHTGHIGDGKIFIYRVADVIKVRTGERGYDALQGVDDL"
BASE COUNT          448 a          380 c          220 g          454 t
ORIGIN      
        1 acatttcaga atattttgta taatcatgcc gttctgatat tccttttcaa taatgccctt
       61 atacagtcct ttgcgaaaca gcttcaacat tttattgtca tccagcctgt atatttcggc
      121 cgtattcccc tggcctatca tttgttcttt ttttatttcc atccatactt tcccgcagac
      181 ataatttttc catttttcat gcgtaaaccg caaatggaat tcacaaaaac atgttcaaat
      241 tatatcataa caattccatt ttgtaccgtg cttcatataa aaaacaggaa aacgaattcc
      301 aaaaagttcg ttttcctgtt tttttctgcc acattataaa tcatcaacac cctgcagtgc
      361 atcatatcct ctttcaccgg ttctgacttt aataacatcg gcaacacggt agataaaaat
      421 cttgccgtca ccaatatgtc ccgtatgcaa tacccttctg gccgtctcaa ccaccttttt
      481 tacaggaacc ttggcgatta ccatctctac tcttacttta ggcagcagct gcatttcaaa
      541 ttcagtacca cggtaaaatt cagttgctcc tttttgcatc ccacagccaa ggatctgcgt
      601 aactgtcata ccagtaacac caatatcatt catggctgct tttaattctt caaacttatt
      661 ctgttttaaa ataatttcca cctttgtaat gtgggcattt tgttcatctc tttcaaaatc
      721 cgcagcggat tcataggatt catatattgt ttgcttctcc gtgatatcct ctatatcttc
      781 accatttgaa tcctgtgcgg ctttatagta attatattta ccgccatagc tgttgatcgc
      841 cgctaccggt aagaaatccg cataagcatt taatagtcca tgttcggatt tttccatgcc
      901 ttccacttca tcctgctctt taacacgcag cccgatcgtt gacttaagaa ccgaaaaaac
      961 aagaaacatt atggctgaaa cccatgtgat cacggaaatc actccaatga tctgcgtcag
     1021 gaagaagaat ccttttccgg tatacaaaag tccattttcc gtcgataata atccgacaag
     1081 tatcgtgccc agagcaccgc atacaccatg tacaccgaca gcgccaaccg gatcatccac
     1141 tttcaggatc ttatcaataa actctacgga aaaaacaact acaattccgg cacataatcc
     1201 aataaaaaac gcaccggtca aagaaaccgt atcacatcct gcagtaatag ctacaagacc
     1261 ggccaatgcc ccgtttaaag tcatggatat atccggcttt ttataacgga tcgcagatgt
     1321 gatcatcgat gtacaggtcg caacggcagc tgccatattc gtattcacaa aaattttaga
     1381 cgccagttct atcgcatctc cttcaaagcg caatgtcgaa cagccattaa atccaaacca
     1441 gccgaaccat aaaataaaaa cacctaaagc actgagtgta atattatgac caggaataac
     1501 tc
//
LOCUS       DAOSYE010000654         1499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75668_length_1499_cov_4.227147, whole genome shotgun sequence.
ACCESSION   DAOSYE010000654 DAOSYE010000000
VERSION     DAOSYE010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75668_length_1499_cov_4.227147"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLU43_10250"
     CDS             <1..454
                     /locus_tag="PLU43_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28833.1"
                     /translation="QLSVAAGFAKNENYEIPVQIDGEWTSVNLKIIRNSEKSGSVSVT
                     METEQYGKVAAQFTVENKKVSGYISGDKKESLESLSQKAAELADTMAGEDRTVEEIQF
                     IPVKQLELNEFTKETSDKEVKTSTKELYEVAKAFITVLQKKGESADEN"
     gene            444..>1499
                     /locus_tag="PLU43_10255"
     CDS             444..>1499
                     /locus_tag="PLU43_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO28834.1"
                     /translation="MKINSNIQAMITNNVLKRNEELLSKSSEKLSSGYRINSAQDNPS
                     GMAITNKMNAQIKSLNKANQNASNAVNAIETAEGAVAEIQEMVQRMNELAVKASSGTN
                     VTADREAIQEEIEQLTAEIERVAGETEYNTQNLLGGEQALKGYTDNAAVDVRDYNTLF
                     KTSDEYSLTFSLDTEGNVTVAGSGFDSTATITTEDGTTTIKTANGGELTIDFDEDAIK
                     QQISLSAAVPQTTTADLDISGVGGMKIQVGSSEGQEIQIVIPEISLENMGIEDIDVST
                     EEGAQAAIDQVTEALSFISSVRSKLGAYENRLETTISNLDVATENLTNSYSAIKDVDM
                     ADEMVEYTKLQVLTQAGT"
BASE COUNT          526 a          265 c          388 g          320 t
ORIGIN      
        1 gcagctttcc gtggcagcag gttttgcaaa gaacgaaaat tatgaaattc cggtacagat
       61 agacggggaa tggacttctg tcaatttgaa gatcattcgt aattctgaaa aaagcggcag
      121 cgtgtcggtt acgatggaaa cggagcaata cggaaaggtg gccgcacagt ttacagtaga
      181 gaataaaaaa gtaagcggat atatttccgg agataaaaag gagagccttg aaagcctttc
      241 gcaaaaagca gcggagcttg cagatacaat ggcaggagag gacagaactg ttgaggaaat
      301 tcagtttatt cctgtaaaac agcttgagct gaatgaattt acgaaagaaa catcagataa
      361 agaagtgaaa acgtcgacga aagaactata tgaagtagca aaagcattta ttacggtatt
      421 acagaaaaaa ggagagagtg cagatgaaaa ttaattcgaa catccaggca atgattacaa
      481 acaatgtatt aaaaagaaat gaggaactgc tttcaaagtc ttcggaaaaa ctttcttccg
      541 ggtaccgaat caacagcgca caggataatc cgtccggcat ggcgatcact aataaaatga
      601 atgcacagat caaaagtctg aataaggcaa atcaaaatgc gtccaatgcg gtcaatgcta
      661 ttgaaactgc agaaggtgcg gttgctgaga tccaggagat ggttcagaga atgaatgaac
      721 ttgccgtaaa agcatcgagt ggtacaaatg taacggcaga cagggaagca attcaggagg
      781 agatcgaaca gctgacggca gaaattgaaa gggttgcagg agagacagaa tacaatacac
      841 aaaatctgct cggcggagag caggcactga aaggctatac cgacaatgcc gcagttgacg
      901 taagagatta taatacctta ttcaagacca gtgatgagta ttcccttacg ttcagccttg
      961 atacggaggg gaatgtgacg gtagcgggca gcggcttcga ctcgacggct acaataacga
     1021 cagaagacgg aacgacaacg ataaagaccg caaatggcgg agaattgaca attgattttg
     1081 atgaggatgc aatcaaacag cagatcagtc tgtctgcggc agttccgcag acaacaacag
     1141 ctgatctgga catatcagga gttggcggaa tgaagatcca ggtcggttct tcggaagggc
     1201 aggaaattca gatcgtgatc ccggaaatat ctttagaaaa tatgggaatc gaggatatag
     1261 atgtatcaac tgaagaagga gcgcaggcag cgatcgatca ggtaacggaa gcattgtcct
     1321 ttatttccag tgtccgttca aagcttgggg cctatgagaa tcgtcttgaa acaacaattt
     1381 caaatctgga tgtggcaact gaaaatctga cgaattccta ttcggcaatc aaagatgtgg
     1441 atatggcaga tgaaatggta gaatatacaa agctccaggt tcttacacag gcaggtact
//
LOCUS       DAOSYE010000655         1499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75671_length_1499_cov_3.722992, whole genome shotgun sequence.
ACCESSION   DAOSYE010000655 DAOSYE010000000
VERSION     DAOSYE010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75671_length_1499_cov_3.722992"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLU43_10260"
     CDS             <1..447
                     /locus_tag="PLU43_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO28835.1"
                     /translation="AAKDITVYAYELTTYILDQKIKKMSVSAAWSDTGVDINNHISIE
                     FAHTLADLTTTFVTKMEEKMVLYGRKGKVVLPCPHFSSECFLYDEKGILTEHFVDKET
                     KNGFTYEILETMNCIKEGKMESSIVPWKVTMTCAELFDEIEKTRKC"
     gene            527..1435
                     /locus_tag="PLU43_10265"
     CDS             527..1435
                     /locus_tag="PLU43_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOO28836.1"
                     /translation="MTINHAILGLLSWKPATGYELKKIFEESSFLYWSGNNNQIYRSL
                     LQLLEEGLVTGELQYQEASPSKKVYTITDEGKEELKKWMLSAPEAPEFKKPFLIQLAW
                     ADLLSEEELKTLFLKYENEIRLCLLMEQEKKRRGLNALDITGRERVLWEKIADNLISS
                     LKGELDWIQDTKDALFENKGEEQPSGIESQVIEKEGQRYLEIVRVKEPVSEEDSALEL
                     IALCGEYDTNRLLLHKEVLADGFFRQKSGIAGRILQKFSNYSVKTAILLSDETAFGRT
                     ENFIKGRNITACLFESREDAEKWLLE"
BASE COUNT          500 a          237 c          369 g          393 t
ORIGIN      
        1 gcggcaaagg atattacggt gtacgcatat gagctcacca cctatattct ggatcagaag
       61 ataaagaaaa tgagtgtgtc ggctgcatgg agtgacaccg gtgtagatat caataatcat
      121 atatccattg agtttgcgca cacgctggct gatctgacaa caacctttgt aacaaagatg
      181 gaagaaaaga tggtactgta tggaaggaag ggaaaagtgg tgctgccttg tcctcatttt
      241 tcatcagaat gttttctcta tgatgaaaaa ggaattctga cagaacattt tgtcgacaag
      301 gagacaaaga atggttttac atatgaaatt ttggaaacga tgaactgtat aaaagaaggt
      361 aaaatggaaa gcagtatcgt accgtggaaa gttacaatga catgtgcaga gctttttgat
      421 gagatagaaa aaacaagaaa atgttagaat gaagagtaga ggttccgctt gacataaggt
      481 atatgataag ttaaagttaa catatgttaa aaatggaggc gggatcatga cgatcaatca
      541 tgcgatatta ggacttctca gctggaaacc ggctacggga tatgagctaa aaaaaatttt
      601 tgaagaatct tcttttttat actggtcggg aaacaataat cagatttatc gttctttgct
      661 gcagcttctg gaagaaggtc tggtaacggg tgaactgcag tatcaggaag cttcaccttc
      721 aaaaaaggtt tatacgatca cggatgaagg aaaagaggaa ctgaaaaaat ggatgctttc
      781 cgcgccggaa gcaccggaat ttaagaaacc gtttcttatt cagcttgcct gggctgattt
      841 gttaagtgaa gaagaattga aaacactctt tttgaaatat gaaaatgaga tcaggctgtg
      901 cctgttaatg gaacaggaga aaaaaagacg cggcttaaat gctttggaca taaccggccg
      961 tgagcgtgta ctttgggaaa aaattgccga caatctgatt tcttccctga aaggtgagct
     1021 ggactggata caggatacga aagatgcact ttttgaaaat aaaggagaag aacagccttc
     1081 cgggatcgaa agtcaggtaa ttgaaaaaga aggacagcga tatttggaaa tcgttcgggt
     1141 aaaagaaccg gtatcagaag aagacagtgc actggaactg attgcgcttt gcggggaata
     1201 tgatacgaac aggctgctgc ttcataaaga agtgcttgcc gatggatttt tcagacagaa
     1261 gtctggaatt gccggcagga tccttcagaa gttttcgaat tattccgtga agactgcaat
     1321 tctgctttcg gatgagactg cttttggcag aacagaaaat tttataaaag gacgaaatat
     1381 taccgcctgc ctgtttgaaa gcagggaaga tgctgaaaaa tggcttcttg aataataaaa
     1441 cgctcatagg aaaagacgca gttttgcttc tccctatgag cgttttaccg gttcattct
//
LOCUS       DAOSYE010000656         1498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75772_length_1498_cov_2.707554, whole genome shotgun sequence.
ACCESSION   DAOSYE010000656 DAOSYE010000000
VERSION     DAOSYE010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75772_length_1498_cov_2.707554"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLU43_10270"
     CDS             complement(<1..1170)
                     /locus_tag="PLU43_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28837.1"
                     /translation="MNRKIKWIMIVIFVTVLAAGCGSASEAGSSLTDGEYQVNVQLTG
                     GSGKASVESPASVAVEDGILTATIVWSSPNYDLMEVGGTSYYPVNDSGNSVFVIEIPA
                     LGQEIEVRAETVAMSEPHMIDYTLLFDFSETAENDSVKDLTGQGSSVIDLEHSDLSYA
                     TQFSLEDYGNGCTLITIIDGSRFLVVPEGEETPADLAEGITVLKQPIKNIYLAATSAM
                     DIFVSLHALDSVKMSGTQTENWAIKEAREAMEENRILYAGKYNEPDYEMILSNQCSLA
                     IESTMIGHAPEVREKLMELGIPVLVDYSSYEAHPLGRSEWIKLYAVLLGEEEQADELF
                     AKQEDLLSAVSKEENTGKTIAFFYISSSGRAIIRKSGDYVSKMLELIALPCLVIIT"
     regulatory      complement(1319..>1498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          366 a          415 c          286 g          431 t
ORIGIN      
        1 agttataatt actagacatg gtaaagcaat aagctccagc atttttgaaa cataatcccc
       61 tgatttacgt ataatggcac ggccggagga actgatataa aaaaaggcga tcgtctttcc
      121 cgtattctct tccttcgata ctgcagaaag cagatcttcc tgtttggcaa acagctcatc
      181 cgcctgttct tcttccccaa gcagcactgc atacagcttg atccattccg atcttccaag
      241 cggatgggct tcatagcttg aataatcaac aagcaccgga attccaagtt ccataagctt
      301 ttccctgact tccggcgcat ggccgatcat tgtcgattcg atcgcaaggg aacattgatt
      361 gcttaaaatc atctcataat ccggctcatt gtatttccct gcatagagga tccggttttc
      421 ttccatcgct tcccttgcct ctttgatcgc ccagttttcg gtctgcgtcc cggacatttt
      481 cacactgtcc agcgcgtgaa gggatacaaa aatatccatg gcagacgttg ctgccagata
      541 aatatttttg atcggctgtt tcagaaccgt gatcccttcc gcaagatctg ccggtgtttc
      601 ttccccctcc ggtacgacaa gaaacctgct gccgtctatg atcgtgatca gcgtacagcc
      661 gtttccgtaa tcctcaagag aaaactgcgt tgcgtacgat aagtctgagt gttccaaatc
      721 aataacggac gacccctggc cggtcaggtc ttttacgctg tcattttctg cagtctctga
      781 aaaatcaaac agcagcgtat aatcgatcat atgaggctcg ctcatcgcaa ctgtttccgc
      841 ccggacctct atctcctgtc ctaatgccgg tatctctatc acaaaaacag aatttccgct
      901 gtcgtttacc ggataatagc ttgttccgcc cacttccatc aaatcataat tcggactgct
      961 ccatacgatc gtagccgtca gtattccgtc ttccacagca acactggccg gagattctac
     1021 ggacgctttc ccggaacctc ccgtcaactg cacattcacc tgatattctc cgtctgtaag
     1081 agaactgccg gcttccgaag cactgccgca gcccgctgca agcaccgtca caaaaataac
     1141 aatcatgatc catttgattt ttctgttcat tttctccacc tataaaaaaa gaccctttac
     1201 cacattggta aaaggtctgc attctctttt cgtacgcaaa atgaaagaat gactgttttc
     1261 attctttctc cgtactacca gttactcgtg gtatgagctt ttgcatattc agcaaaagca
     1321 tccgtaccgg acgataggca ggtctcctga cttacagatc ggcatattct tcgtcttccc
     1381 agtctcccag tgacttatcg gtcatctccg attgaagaat acttcctgtt tacagggacg
     1441 agttcgcaca ggattttcac ctgtttccct tttcaaccgg atgcgtttca cgtccggt
//
LOCUS       DAOSYE010000657         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75915_length_1496_cov_3.373352, whole genome shotgun sequence.
ACCESSION   DAOSYE010000657 DAOSYE010000000
VERSION     DAOSYE010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75915_length_1496_cov_3.373352"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLU43_10275"
     CDS             <1..587
                     /locus_tag="PLU43_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28838.1"
                     /translation="RALGGGKAIGLLKENFYDIIFIDHKMSKINGLDAAKIIRAMPGE
                     YYKTVPLVFCTDEKMDNIYKDFLEAGFSDYLLYPISVKHLSLVLTRWVWKKLEKQEAL
                     RKEEEVWDGNHREIEVLLNDSLMFYEKKNMMLFSFCIRGIKQMSMIIGSSNAEELARE
                     AGNAILFEDMDMLEETYMELVKVLKALLKENGEA"
     gene            complement(606..>1496)
                     /locus_tag="PLU43_10280"
     CDS             complement(606..>1496)
                     /locus_tag="PLU43_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HOO28839.1"
                     /translation="HIPVISLNLSKLESNPGFKMDAKLLTRGVYAAVFGDIFMRCLYR
                     MRPYEKVPGSADALHQKWVVRCQEFISGSHPSFHTYKKMCRQIIRDFDALPVTEEKKP
                     RVGVVGEILVKFSPLANNDLVNLIEAEGAEAVVPDLLDFMQYCFYNQIYKAEHLGTKR
                     SSAIFGQLGIHAINFIRGSATKAFKESKHFSSAVPIETLAEYASTIVSIGNQTGEGWF
                     LTGEMMELIHSGVGNIVCTQPFGCLPNHVVGKGVIKALRRSYPQSNIVAIDYDPGASE
                     VNQLNRIKLMLSTAFKNLEE"
BASE COUNT          433 a          295 c          367 g          401 t
ORIGIN      
        1 ccagggcgct cggcggcgga aaagcgatcg ggcttttgaa ggaaaatttc tatgatatta
       61 tatttattga tcataagatg agtaaaataa atgggcttga tgcggcaaaa ataatcaggg
      121 cgatgcctgg tgaatattat aaaacagtac cgcttgtttt ttgcacggat gaaaaaatgg
      181 ataatattta taaggatttt ttggaagccg gtttcagcga ttatctgcta tatccgattt
      241 ccgttaagca cctgtcactt gtactgacac gctgggtgtg gaaaaaactt gaaaaacagg
      301 aagcactccg gaaagaggaa gaagtatggg acggcaatca tcgtgaaata gaagtgcttc
      361 ttaacgacag tctcatgttt tatgaaaaga agaatatgat gttgttttct ttttgtatca
      421 ggggaatcaa acagatgagt atgatcattg gcagttccaa tgcggaggag cttgcaagag
      481 aggccggaaa tgctattttg tttgaagaca tggatatgct tgaggagacg tacatggagc
      541 ttgtgaaagt gctgaaagca ctgttaaaag aaaacggaga agcatgaacg acttctccgt
      601 ttttactatt cttcaagatt tttaaatgca gtcgacagca tcagcttgat ccggttcagc
      661 tgattgactt cactggcacc tgggtcataa tcgatggcaa caatgttgga ttgcggatag
      721 ctgcggcgca gggctttgat aacacctttc ccgacaacgt gattcggcag acacccgaat
      781 ggttgtgtac agacaatatt tccaacaccg gaatggatca gttccatcat ttcgcctgtc
      841 aggaaccagc cttctccggt ctgattgcca atggatacaa ttgttgaagc atactccgca
      901 agcgtttcga tcggaaccgc agaggaaaaa tgtttgcttt ctttaaatgc ctttgttgcg
      961 gagcctctga taaaattaat ggcatggata ccaagctgcc cgaaaattgc agaggaacgt
     1021 ttggtcccca gatgttccgc tttgtagatc tgattgtaga agcagtactg cataaaatcg
     1081 agcaggtccg gtacgacggc ttctgcgcct tctgcttcga tcagattaac aagatcgtta
     1141 ttggcaagcg gagaaaattt gacaagaatt tcaccgacaa cgccgacgcg cggctttttt
     1201 tcttccgtaa caggcaatgc gtcaaaatcc cggatgatct gcctgcacat ttttttgtac
     1261 gtatgaaagg acggatgaga acctgaaata aattcctgac agcgtacgac ccatttttga
     1321 tgcagggcat cggcagaacc aggtactttt tcgtatgggc gcatccgata gagacaacgc
     1381 atgaaaatgt caccgaatac tgcggcataa acaccccgtg tcagcagctt tgcatccatc
     1441 ttgaagccgg gatttgactc cagcttggaa agattgaggg aaataacagg tatatg
//
LOCUS       DAOSYE010000658         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75919_length_1496_cov_3.247051, whole genome shotgun sequence.
ACCESSION   DAOSYE010000658 DAOSYE010000000
VERSION     DAOSYE010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75919_length_1496_cov_3.247051"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLU43_10285"
     CDS             complement(<1..960)
                     /locus_tag="PLU43_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOO28840.1"
                     /translation="MINIFEVNETNQMIEKENLDVRTITLGISLLDCITDDLDSLRKN
                     IYEKITTTAKDLVATGKEIETEFGIPIVNKRISVTPIALVGSAACKTPEDFAAVAKTL
                     DDAAKQVGVNFIGGYSALVSKGMTREEENLIRSIPLALSITDRVCSSVNLGSTKTGIN
                     MDAVRLMGEIVKKTAEATKENDSLGCAKLVVFCNAPDDNPFMAGAFHGVTEADAVINV
                     GVSGPGVVKTALSKVRGESFEVLCETIKKTAFKITRVGQLVAGEASRRLGIPFGIIDL
                     SLAPTPAVGDSVADILCEIGLEYAGAPGTTAALALLNDQVKKGG"
     gene            complement(985..1257)
                     /locus_tag="PLU43_10290"
     CDS             complement(985..1257)
                     /locus_tag="PLU43_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO28841.1"
                     /translation="MKKTIITVVGKDTVGIIAKVCTYLANNRINILDISQTIVQDYFN
                     MMMIVDMNESSKPFGIVASELEQIGEEIGVIVKCQREEIFSKMHRI"
     gene            complement(1282..>1496)
                     /locus_tag="PLU43_10295"
     CDS             complement(1282..>1496)
                     /locus_tag="PLU43_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO28842.1"
                     /translation="NELSGKLLCRLLHDKLKMVLLGHLSAENNLAELAYETVRLEILM
                     SDSGYKPEDFKICVAGRTQCSGLIAL"
BASE COUNT          374 a          401 c          276 g          445 t
ORIGIN      
        1 accgcctttc tttacctgat cattcagcag tgcaagcgct gccgtcgttc cgggtgctcc
       61 tgcatattcc agtccgatct cgcataaaat atccgcaaca ctgtctccga cagccggtgt
      121 cggcgcaaga gataaatcaa taataccaaa tggaatccca agccttcttg aagcttcacc
      181 ggcaacaagc tgaccgaccc gcgtgatctt aaatgcggtt tttttgatcg tttcacataa
      241 cacttcaaag ctttcaccgc gcactttgga gagtgctgtc tttacaacgc ccgggccgct
      301 gactccgaca ttgataacag cgtccgcttc ggtcacacca tgaaacgcac ctgccataaa
      361 tggattatca tccggtgcat tgcagaacac aacaagcttt gcgcagccga gagaatcgtt
      421 ttcctttgtc gcttccgctg tctttttaac gatctccccc atcagtctga ctgcatccat
      481 gttgatcccg gtctttgtcg aacctaaatt gacagaactg cagaccctgt ctgttattga
      541 tagcgcaagc ggaatcgatc ggatcaggtt ttcctcttcc cttgtcattc cttttgacac
      601 aagtgcggaa taaccgccga taaaattaac gccgacctgt tttgccgcat catcaagtgt
      661 tttagcaact gctgcgaaat cctcaggcgt tttacatgcc gcagagccga caagtgcgat
      721 cggggttacg gaaatacgtt tattaacgat cgggatccca aattccgttt caatttcttt
      781 gcctgttgca acaagatcct ttgctgtcgt tgtgatcttt tcataaatat tttttctaag
      841 actgtccaga tcgtctgtta tacagtcaag cagactgatt ccaagcgtaa tcgtccggac
      901 atccagattt tctttttcga tcatctgatt ggtttcgttc acttcaaata tattaatcat
      961 gagagctctc cttatggatc ttacttatat acggtgcatt ttactgaata tttcttcacg
     1021 ctggcatttt acgatcacac cgatctcttc gccgatctgt tccagctcgg acgctacgat
     1081 tccaaacggt ttgctggact cattcatgtc aacgatcatc atcatattaa aataatcctg
     1141 tacaatcgtc tgagaaatat ccagaatatt gatcctgtta ttggctagat acgtacacac
     1201 ctttgctatg ataccaaccg tatcttttcc cactaccgta ataattgttt ttttcataat
     1261 cttttctcct cgtttcctaa atcaaagggc aatcagcccg gaacactgcg ttctgcccgc
     1321 aacacagatc ttaaaatctt ccggcttata tccactgtcc gacatcaata tttcaagccg
     1381 cacagtctca taggcaagct ccgcaagatt attctcagca ctcagatgac cgagcagcac
     1441 cattttcaat ttatcatgca gcagccgaca gagcagcttt cccgaaagct cattgg
//
LOCUS       DAOSYE010000659         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_75932_length_1496_cov_2.770992, whole genome shotgun sequence.
ACCESSION   DAOSYE010000659 DAOSYE010000000
VERSION     DAOSYE010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75932_length_1496_cov_2.770992"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1065)
                     /locus_tag="PLU43_10300"
     CDS             complement(190..1065)
                     /locus_tag="PLU43_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO28843.1"
                     /translation="MKFGALEAGGTKMVCAVGLEDGTILEQVSIPTKTPGETIPHIIE
                     YFKDKEIASLGIGAFGPVDVNKTSETYGRILDTPKLAWQRYDLVGDLKAALNIPIELD
                     TDVNGSCLGEVTFGCAKGLSTVIYITIGTGVGVGVAINGNLLHGMLHPEAGHIILKKH
                     AEDSYEGHCPFHKTCFEGMASGPAIEARWGEKAVSLKDRPEVWEMEAYYIAQALTSYI
                     FTLSPEKIILGGGVMHQEQLFPMIRKKTLELVNGYINTKQLRDIDHYIVPASLQDNQG
                     IMGAIQLAKWAAGIQ"
     gene            complement(1210..>1496)
                     /locus_tag="PLU43_10305"
     CDS             complement(1210..>1496)
                     /locus_tag="PLU43_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOO28844.1"
                     /translation="KSLDVMQIPYREADRHTNGIPGRLVSCDFYTVDRQQIEMEQEFV
                     QNKPFQIVSVIEGEGVIDGNSIETGDSFIIPFDYGTYKITGKITVLITSP"
BASE COUNT          377 a          392 c          269 g          458 t
ORIGIN      
        1 catattttta taattataat aaacaacgcc ggccactgca gccaggatcg ccagtaccag
       61 caagatcctg tacaggatcg ccaggcggtg tctggtcagc ttttttctat agtctttctt
      121 ttcagcactg tatttatttt taaaatattc attgatctgt gccattattt ttctccgggc
      181 aatattctgt tattggatcc cagctgccca ttttgccagc tggatcgcac ccatgattcc
      241 ctgattatcc tgcagacttg ccggcacgat ataatgatcg atatccctca gctgctttgt
      301 attgatatag ccattcacca gttccagtgt ctttttgcga atcattggaa acagctgttc
      361 ctgatgcata acgccgccgc cgagtatgat cttttccgga gataaggtga agatataaga
      421 agtcagcgcc tgcgcaatat aatacgcttc catctcccag acctccggtc tgtcctttaa
      481 agaaactgct ttttctcccc acctggcttc aatcgccgga ccggatgcca tgccctcaaa
      541 gcaggttttg tggaacggac aatgtccttc gtaagaatct tcagcgtgtt ttttcaaaat
      601 aatgtgaccg gcttccggat gcagcattcc atgcagtaag tttccgttaa tggcaacacc
      661 gacaccaaca cctgttccga tcgtaatata aatgacggta ctaagacctt ttgcacatcc
      721 aaaagtcacc tctccaagac aggagccgtt tacatccgta tcaagctcta tcggaatatt
      781 aagtgcggcc tttagatccc ctacaagatc atatctctgc catgcaagct tcggcgtatc
      841 cagaatcctt ccataggtct ccgatgtctt attgacatcg accggaccaa atgctccgat
      901 tccaagagag gcgatctctt tatctttaaa atattcgata atatgaggaa tcgtttcccc
      961 tggtgttttg gtagggatcg aaacctgttc caaaatcgta ccgtcttcca gtccgacagc
     1021 acataccatc tttgtgcccc ctgcttccaa tgctccaaat ttcatattct tctccttcac
     1081 tttccctttt attaaggtct tctgaaaacc cagacctctc aaagcctttc ataaaaatac
     1141 tgcagccacc agatacatgc tgcatccggc ggctgtccgt agcctgtatc aaacactatt
     1201 ataatgcatt tacgggcttg taatcaatac tgtaattttt ccggtgattt tatatgttcc
     1261 gtaatcaaac ggaataataa aggaatcccc tgtttcaatg ctgtttccgt caataacgcc
     1321 ttctccttcg ataacgctta cgatttgaaa aggcttattc tgaacaaatt cctgctccat
     1381 ttctatttgc tgtctgtcaa ccgtataaaa atcacaggat acgagcctcc ccggtatccc
     1441 attcgtatgt ctgtctgcct cccgatacgg aatctgcatg acatcaaggc tttttt
//
LOCUS       DAOSYE010000660         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76163_length_1493_cov_3.374131, whole genome shotgun sequence.
ACCESSION   DAOSYE010000660 DAOSYE010000000
VERSION     DAOSYE010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76163_length_1493_cov_3.374131"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1493)
                     /locus_tag="PLU43_10310"
     CDS             complement(55..>1493)
                     /locus_tag="PLU43_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOO28845.1"
                     /translation="IEDGQIVMQRKIMNGRGICKINGETVPVSVLKEVSGLLIDIHGQ
                     HEHQSLLYKKKHREILDSYCAEAFAGLPARILEYYGQYIKKRAELADAEKTTDNKEKE
                     AALAQFEVDEIREADLIIGEDEQLEADYRRMKNAKKIAEAISFVHQYTGYDSENGAGA
                     AIGRALGFLRNAAGYDEEMAQLLAQITDIDNLLNDFNRSVAVYEEKMTFGEEEFIRTE
                     ERLNTINHMKSKYGDTIEKIMAYCEEQEKSLEKYQNYDEYIGNLRRETEHLKEQLTDV
                     CEKVSKIRSREAVILAQEIKDALIDLNFSDARFEIRVEKSEEHMSVNGFDDVEFLIST
                     NPGERVKPLIQVASGGELSRIMLALKAVLAGKDQIGTLIFDEIDTGISGRTAQKVSEK
                     LAYLSGKHQVICVTHLPQIAAMADVHFEIAKSVNNGRTVTEVKKLDEEASVKELARML
                     GGVKITEAVFENAKEMKKLAGKIKKKFL"
BASE COUNT          342 a          399 c          243 g          509 t
ORIGIN      
        1 tgaattccga ttttaatttt gttttttaaa gcaaaatttt tatttgacct gtttttacaa
       61 aaactttttc tttatcttcc ctgccagctt cttcatttcc ttcgcatttt caaaaactgc
      121 ctcagtgatt tttacaccgc cgagcattct ggcaagttct tttacggatg cttcttcatc
      181 caattttttt acttccgtta ccgtcctccc gttatttaca ctctttgcta tttcaaaatg
      241 gacatccgcc attgctgcga tctgcggtaa atgcgtgaca cagatcacct gatgctttcc
      301 agacagataa gccagctttt ccgaaacctt ttgtgcggta cgcccgctga taccggtatc
      361 gatctcgtca aaaatcagcg taccgatctg gtcttttcct gcaagaactg ccttcagtgc
      421 cagcatgatt ctggataact cgccgccgga agctacctgg atcaatggtt tgactctctc
      481 tccgggattt gtgctgatca gaaattccac atcgtcaaaa ccattcacgc tcatatgctc
      541 ttcggatttt tccacacgga tctcaaatct ggcatccgaa aaattcagat caatcagggc
      601 atctttaatt tcctgtgcca gaataacggc ttccctgctt ctgatttttg aaaccttctc
      661 acaaacgtct gtcagctgct ccttcagatg ctctgtttcc cttcgtaaat ttccgatata
      721 ttcgtcataa ttctgatatt tttccagact tttttcctgc tcttcacaat aagccatgat
      781 tttttcgatc gtatcgccat acttgctctt catatgattg atcgtattca gtctttcttc
      841 cgtccggata aattcctcct cgccaaaggt catcttctcc tcataaacgg caaccgatct
      901 gttgaaatca ttcagcagat tatcaatatc ggtaatctga gcaagcagtt gcgccatctc
      961 ttcatcataa cctgccgcat tccgcaaaaa accaagcgcc cgtccgattg cggctccggc
     1021 tccgttctca ctgtcatatc ccgtatattg atgtacaaaa gaaatcgctt ccgctatttt
     1081 ttttgcattt ttcatcctgc gataatcagc ttccagctgt tcatcttcac caatgatcaa
     1141 atcagcttct cttatttcat caacttcaaa ctgggcaagc gcagcttcct tttctttgtt
     1201 atcagttgtt ttttccgcat ccgcaagttc tgctcttttt tttatatatt gcccataata
     1261 ctctaaaatc cgggcaggca ggccggcaaa tgcttccgcg cagtaactgt ccagtatttc
     1321 cctgtgcttt tttttatata aaagagactg atgttcatgc tgcccatgaa tatcgatcag
     1381 cagtccggaa acttccttta atacggacac cggcacagtc tccccgttta ttttgcaaat
     1441 accgcgtccg ttcatgatct tgcgctgcat aacgatctgc ccgtcttcga ttg
//
LOCUS       DAOSYE010000661         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76205_length_1493_cov_2.003477, whole genome shotgun sequence.
ACCESSION   DAOSYE010000661 DAOSYE010000000
VERSION     DAOSYE010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76205_length_1493_cov_2.003477"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PLU43_10315"
     CDS             complement(<1..1479)
                     /locus_tag="PLU43_10315"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOO28846.1"
                     /translation="MALQLWMGNAGSGKSYGLYQHIIKESEAHPELTYLVVVPEQFTL
                     QTQRDLVMLHRKHGIMNIDILSFARLAYRVFEEVGFAGASGILIDDMGKSLILRRLAG
                     QYEDRLQVLGKNLKRLGYINEVKSVISEFMQYGIQNEEIDLLKQRAETSGKAMLNRKL
                     SDIQVLFNAFRGYIDEKYTTSEEILERVSGILPDSEKIKRSVIVFDGFTGFTPVQYKL
                     IAALLRASVDVYMTILLDTKENPYVTTGEQELFYLSKKTIRQMTGIAESLQVERKPDH
                     VINEPVPIRFQYASGMLAHLEKNLFRPDAKSFEKPKEEDEAAAISIFTGTNPEEEMKN
                     TAVRILSLIQEQGYRYRDIAIVTGDIDTYMHAAQRVFTRYGIPYFVDKTQPILLNPFI
                     EYMRAVISILADDYSYEGMFRYLKSSLTEFDLMEIDRLENYVLACGIKGSGQWNRHFI
                     RKTASMKTEELLALDVLRAKIVHAFEPFHDIFNRTASVKSFAN"
BASE COUNT          379 a          394 c          285 g          435 t
ORIGIN      
        1 attcgcaaag cttttcacag acgccgtacg gttgaaaata tcgtgaaatg gttcaaaagc
       61 atgtactatt ttagcccgca gtacatccag cgcaagcagt tcctccgttt tcatcgaagc
      121 cgtctttctg atgaaatgcc ggttccactg tccgctgccc ttgattccgc atgcaagcac
      181 ataattctca agacggtcga tctccattaa atcaaactcc gtaagggaag acttcagata
      241 ccggaacatt ccttcgtaag aataatcatc cgccagaatc gaaatgactg cacgcatata
      301 ctcaataaac ggattcagca ggatcggctg tgtcttatct acaaaatacg gaatcccgta
      361 tcttgtaaat acccgctgtg ccgcatgcat atatgtatca atatcacccg ttactattgc
      421 aatatcccga tagcggtatc cctgctcctg tatcagcgat aagattctga ctgctgtgtt
      481 tttcatttcc tcttccggat tcgtaccggt aaaaatactg atcgctgctg cctcgtcttc
      541 ttcctttggc ttttcaaacg attttgcgtc aggtctgaaa agattttttt caagatgcgc
      601 aagcatgccg gatgcatact gaaagcgtat cggaaccggt tcattgatca catgatccgg
      661 ttttctttcc acctgcagcg attccgcaat tccggtcatc tgccggatcg tctttttact
      721 cagataaaac aattcctgct ctccggtcgt cacatatgga ttttccttcg tatcgagaag
      781 aattgtcata taaacatcca cactggcgcg cagcagggca gcgatcagtt tatactgtac
      841 cggtgtaaag cccgtaaacc cgtcaaatac gatcacactt cttttgattt tttcagaatc
      901 cggcaggatc ccgctcaccc gctctaagat ctcttcgctg gtcgtatatt tttcatcgat
      961 gtaaccgcga aacgcattaa ataacacctg tatatcgctt agtttccggt tcagcattgc
     1021 ttttccggat gtttccgccc gctgttttaa caggtcgatc tcttcattct gaataccata
     1081 ctgcataaat tccgaaataa cagatttgac ttcattgata taaccgagtc ttttcaggtt
     1141 ttttccaagc acctgcagcc tgtcttcgta ttgtccggcc agacggcgca gtatcaggct
     1201 ctttcccatg tcatctatta aaatgccgga cgcaccggca aaacctactt cctcaaatac
     1261 gcgataagca agacgggcaa aactcagaat gtcgatattc atgatgccat gctttcgatg
     1321 cagcataaca agatcacgct gcgtctgcag tgtaaactgc tccggaacaa cgaccagata
     1381 agtcaattcc ggatgtgcct cagactcttt gattatgtgc tgataaagac cgtatgattt
     1441 tccgcttccc gcatttccca tccatagctg cagcgccata tctgtcctcc gtc
//
LOCUS       DAOSYE010000662         1492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76261_length_1492_cov_2.792624, whole genome shotgun sequence.
ACCESSION   DAOSYE010000662 DAOSYE010000000
VERSION     DAOSYE010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76261_length_1492_cov_2.792624"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..177)
                     /locus_tag="PLU43_10320"
     CDS             complement(43..177)
                     /locus_tag="PLU43_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOO28847.1"
                     /translation="MRSERELFDLILGTGKTDARIRVVYMNGSRTNVNAPKDIFHNVI
                     "
     gene            complement(205..978)
                     /locus_tag="PLU43_10325"
     CDS             complement(205..978)
                     /locus_tag="PLU43_10325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO28848.1"
                     /translation="MVFEVRQPETVVPLFAGWQETCIWSCLQKIMGHLYVTDTDRPQS
                     VMAILGDFCFFAGVPDKELVSFKPQWCRNNFMIMVPQTMQWEKQIEDFYGERAKKVVR
                     YAIKKEPGIFDKEKLQKVVGSLSDEFCLRMIDEELFDYCQNTAWCRDWVSQYDSFEQY
                     QEIGLGAVVMKDGEPVSGASSYSGYQDGIEVEIITKETYRRRGLAYICGAQLILACME
                     RNWYPSWDARNKWSVALSEKLGYHFDHEYPVYEISDYES"
     gene            complement(1045..1284)
                     /locus_tag="PLU43_10330"
     CDS             complement(1045..1284)
                     /locus_tag="PLU43_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28849.1"
                     /translation="MAEKDNLRTGRIIGKLERVIIAILVLCNQFAAIGFVLTAKSIAR
                     FKQLEDKEFAEKYLIGTLASVLIALIAAIVLRQAL"
     gene            complement(1292..>1492)
                     /locus_tag="PLU43_10335"
     CDS             complement(1292..>1492)
                     /locus_tag="PLU43_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28850.1"
                     /translation="RRDFNLLLHPIVIRRLTLKCGKNFYLRTGRAMALFQQAGRLCFV
                     IRYNMGSGCGFYKEAVFVYDRQ"
BASE COUNT          425 a          402 c          234 g          431 t
ORIGIN      
        1 ctgttgaatg tgatatacga tttttatcta aaaaatgctt atctaaatga cattgtgaaa
       61 aatatccttt ggtgcattca cattcgttct ggatccattc atataaacaa cgcgaatccg
      121 cgcatccgtt ttccctgtac ccagaatcaa atcaaacagt tccctttcac tcctcatttt
      181 catctctccg tcattgtacc ctcatcagct ttcataatca ctgatctcat acaccgggta
      241 ctcatggtca aaatggtaac ccagcttttc ggacaatgct accgaccatt tatttctggc
      301 atcccagctt ggataccagt tacgctccat gcacgcaaga atcagctgtg caccgcagat
      361 ataagcaagt cctcttctcc gatacgtctc cttcgtaatg atttccacct caataccatc
      421 ctgatagcct gaataagaag aggctccgga taccggttct ccgtccttca taaccacagc
      481 tcccagtccg atctcctgat actgttcaaa actgtcgtat tgtgagaccc agtctctgca
      541 ccatgcggta ttttgacaat aatcaaacaa ttcctcgtct atcatccgca ggcaaaattc
      601 atcagataaa gaaccaacaa ctttttgcag cttctcttta tcaaaaatac ccggttcctt
      661 ttttattgca taccgaacga ccttcttcgc cctttcacca taaaaatctt ctatctgttt
      721 ttcccattgc attgtctgcg gaaccatgat cataaaatta tttctgcacc attgcggttt
      781 aaaagacaca agctctttat cgggcactcc tgcaaaaaaa cagaagtcac ctaatattgc
      841 catcactgac tgcggccggt ctgtatctgt cacatataag tgccccatta ttttctgcag
      901 acacgaccat atgcatgttt cctgccagcc tgcaaacaaa ggaactactg tctccggctg
      961 tctgacttca aaaaccatag taaaccctcc ataaatcatg cggactgatg tccgtctcct
     1021 atttcaacaa catttccatc cgggtcatag agcctgtctt aaaacaattg ccgcaataag
     1081 tgcaatcaat acactggcca atgtacctat cagatatttt tccgcaaatt ccttatcttc
     1141 aagctgctta aaacgggcaa tactctttgc ggtcaataca aatccgatcg ctgcaaactg
     1201 gttgcacagt actaaaatcg cgatgatcac acgctcaagc tttccgataa tacgtcctgt
     1261 ccgcagatta tctttctccg ccatagggct gtcattgccg gtcatatacg aaaacagctt
     1321 ctttataaaa accgcagccg gatcccatat tataacgaat aacaaaacat aaacgaccag
     1381 cttgctgaaa tagggccatt gctctgcctg ttcgtaaata aaagtttttt ccgcacttaa
     1441 gtgttagacg gcgaattaca atcgggtgca acagcaaatt aaaatcccgt ct
//
LOCUS       DAOSYE010000663         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76580_length_1488_cov_2.928821, whole genome shotgun sequence.
ACCESSION   DAOSYE010000663 DAOSYE010000000
VERSION     DAOSYE010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76580_length_1488_cov_2.928821"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLU43_10340"
     CDS             complement(<1..747)
                     /locus_tag="PLU43_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO28851.1"
                     /translation="MSFTTKTTKKQKANEYTVKITWKEIKRQKVLLIWSAIIVAYGIV
                     FYYLPLVGWAMAFENYKPKDGILHSAFIGLAKFKTLFSDATFIRVIRNTLAMGIINLV
                     VTFIMAIVFAILLNEIRFIPGKKAVQTISYLPHFLSWIIVTGILHDALSGSGIVNEIL
                     VKLNVIDQTINFFAYPKYFWAIVAFANVWKETGWNAIIYLAAITAIDPSLYEAASIDG
                     AGRWNKIKYVTLPGIKPTIIILLLMNVGNVL"
     gene            complement(821..>1488)
                     /locus_tag="PLU43_10345"
     CDS             complement(821..>1488)
                     /locus_tag="PLU43_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO28852.1"
                     /translation="NQWHNSGGVLNVSSGIAITEDCDDVEGAMQFINDLLDQDVHDLR
                     FWGVQDTDYMTDENGEYYRDDTMRLNAADTSYKASHLCQYSYFPQYNGTSKDEINAMK
                     PEGQANEFAIGLNDDVKECFDAYGCETYVDMIGTSEAPGAWYPMWSYSNTLTTATEGG
                     TAWTKMGEIKHEYLPKVVMASDFESAWDDYMVVYDECNPQAFLDEMQTELDRRMAEAA
                     EYE"
BASE COUNT          408 a          361 c          263 g          456 t
ORIGIN      
        1 cagtacgttc ccgacattca tcagcaataa aataatgatc gtcggtttga taccgggaag
       61 tgtaacatat ttgattttat tccatcgccc tgcaccgtcg atcgatgcgg cctcataaag
      121 cgacgggtca attgccgtaa ttgccgccag ataaataatg gcattccagc ctgtctcttt
      181 ccatacgttc gcaaaggcta cgatcgccca gaaatacttt ggatatgcaa aaaaattgat
      241 ggtctggtct atcacattta attttacgag aatctcattg acaataccgg agcctgataa
      301 tgcatcatgc agaataccgg ttactatgat ccatgacaaa aagtgaggaa gataggaaat
      361 tgtctggact gcttttttac cgggtataaa tcggatctca tttaacaaaa ttgcaaatac
      421 gatcgccata ataaacgtta caactaaatt aatgatgccc attgccagtg tattgcgaat
      481 gacccgtata aaggttgcat ctgaaaacag tgtcttgaat ttcgcaagtc cgataaatgc
      541 agaatgaaga attccgtctt tgggcttata attttcaaat gccattgccc agccgactaa
      601 cggcaggtaa taaaatacga ttccataggc tacgataatt gccgaccaga tcaaaagtac
      661 tttctgtctc ttgatctctt tccatgtgat ctttaccgta tattcgttcg ctttttgttt
      721 tttcgtggtc tttgttgtaa aagacatgca ttttctcctt aaaaaaacag gcagatgaaa
      781 ttccatatat ctgcctgttt taaaattcca tttctattct ttattcatat tctgctgctt
      841 ccgccattct gcgatccagt tccgtctgca tctcatccaa aaatgcctgc ggattacact
      901 cgtcataaac aaccatgtag tcgtcccatg cgctttcaaa atcagaagcc attacgacct
      961 tcggcaaata ctcatgcttg atctctccca tctttgtcca tgccgtaccg ccctcggtag
     1021 ctgttgtcaa tgtattggaa taactccaca tcggatacca tgcgcccggt gcttcgctcg
     1081 tgccgatcat atccacgtat gtttcacatc catatgcatc aaagcattcc tttacatcat
     1141 catttaaacc gatcgcaaat tcgttcgcct gtccttccgg cttcatcgca ttgatctcat
     1201 ccttgcttgt tccgttatac tgcggaaaat aggaatactg gcataaatga gaagctttat
     1261 acgatgtatc cgctgcgttg agtctcattg tatcgtcacg atagtattct ccgttttcgt
     1321 ctgtcatata atccgtatcc tgtacacccc agaaacgaag atcatgcaca tcctgatcaa
     1381 gcagatcatt gataaactgc atagcgcctt ccacatcatc acagtcttcc gtaattgcaa
     1441 taccgcttga tacattcagt acaccgccgg aattgtgcca ctgattct
//
LOCUS       DAOSYE010000664         1487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76641_length_1487_cov_3.080307, whole genome shotgun sequence.
ACCESSION   DAOSYE010000664 DAOSYE010000000
VERSION     DAOSYE010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76641_length_1487_cov_3.080307"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLU43_10350"
     CDS             <1..428
                     /locus_tag="PLU43_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO28853.1"
                     /translation="GQVNIAEEVLSSMNLQIPVCGMVKDDNHRTRGLYYLNREIPIDR
                     SSEGFRLVTRIQDEAHRFAIEYHRSLRNSSQVHSVLDDIPQIGGTRRKALMRHFSSIE
                     EIRAASEASLAGIPSMNALSAKRVYDFFHPKEVESQKKV"
     gene            489..1427
                     /gene="hprK"
                     /locus_tag="PLU43_10355"
     CDS             489..1427
                     /gene="hprK"
                     /locus_tag="PLU43_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOO28854.1"
                     /translation="MATVAVSILIEKMKLHNFTPEIDIKGIKITQPDINRPALQLAGY
                     IEHFEATRVQIIGFVEYTYMESLSEEVRRERYEHLLSGATPCVVFCRELKPDDIFLEV
                     ARKNNVPVLSTKKATSNFMAEIIRWLNVKLAPCISVHGVLVDVYGEGVLITGESGIGK
                     SEAALELVKRGHRLVTDDVVEIRKVSDDTLIGSAPDITRHLIELRGIGIVDVKMLFGV
                     SSVRDTQSIDLVIRLEEWDKDKDYDRIGLEEEYTEYLGNKVVCHNIPIRPGRNLAIIC
                     ESAAVNFRQKKMGYNAAKELYSRVQNSLNKRSEEDE"
BASE COUNT          450 a          258 c          400 g          379 t
ORIGIN      
        1 aaggacaggt caatatcgca gaagaagtgc tttcttccat gaatctgcag attcccgtct
       61 gcggtatggt aaaagatgat aatcacagaa cgagaggact ctattatttg aacagggaaa
      121 ttccgatcga ccgcagttcg gaaggattcc gccttgttac aaggattcag gatgaagcgc
      181 accggtttgc gatagaatac caccgttcgt taagaaacag cagccaggtg cattctgttc
      241 tggatgatat tccgcagatc ggcgggacgc gcaggaaggc gttgatgagg cacttttctt
      301 cgattgaaga gatcagggct gccagtgaag catcgctggc aggaataccg tccatgaatg
      361 cgctttctgc gaagcgcgtt tacgattttt ttcatccgaa agaggtagaa tcgcaaaaaa
      421 aagtgtgata caataaaaaa tgagcggtat acgccggaat tatagaaaat tcgagaagga
      481 gattatatat ggcaacggta gcggtaagca tattaattga aaaaatgaag cttcataatt
      541 tcacaccgga aattgatatt aaggggatca agattaccca gccggatatt aaccggcctg
      601 cacttcagct ggcgggatat attgagcatt ttgaggcaac acgtgttcag attatcggat
      661 ttgttgaata tacttatatg gaatcattaa gtgaggaagt gcgcagggaa cgctatgagc
      721 atctgctctc gggagcgacg ccgtgtgttg tattctgcag ggaattaaag cccgatgata
      781 tttttctgga ggttgcccga aaaaataatg taccggtgct ttctacgaaa aaagccacat
      841 caaactttat ggcagaaatt atccgctggc tgaatgtgaa gctggcaccg tgtatttccg
      901 tacatggtgt tcttgtggat gtatatggtg aaggagttct gatcacgggt gagagcggga
      961 tcggaaagag tgaagctgct cttgaacttg tcaagagagg acatcgtctt gtaacggatg
     1021 atgtggtaga gatcagaaaa gtcagcgacg atacgctgat cggctctgcg cccgatatta
     1081 caaggcatct gatcgaactt agaggaattg gaattgttga tgtaaagatg ctgtttggtg
     1141 tatccagtgt acgtgataca cagtcgatcg atcttgtcat ccgccttgaa gagtgggaca
     1201 aggataagga ttatgacaga atcggacttg aagaagagta tacggaatat cttgggaata
     1261 aagtggtgtg ccataatatt ccgatcagac caggccggaa tcttgcgatc atctgcgagt
     1321 cggctgcggt taatttccgg cagaagaaaa tgggttataa tgcagccaag gagctgtaca
     1381 gcagagtaca gaattcatta aacaagcgtt ctgaggaaga cgaataagag tataaataaa
     1441 gatttaaagg agaataaaaa tggatagata ctgctttggt gttgatg
//
LOCUS       DAOSYE010000665         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_76741_length_1486_cov_2.777079, whole genome shotgun sequence.
ACCESSION   DAOSYE010000665 DAOSYE010000000
VERSION     DAOSYE010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76741_length_1486_cov_2.777079"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..508)
                     /locus_tag="PLU43_10360"
     CDS             complement(53..508)
                     /locus_tag="PLU43_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28855.1"
                     /translation="MSISKQTEQEIGIEALIRETEAHTLLFAPANLKEEILIKSRSVS
                     VQVPLRLQKQKKEFSKQMQFLFYTFKISAAVIICLCQLIAIEKISPGLTFSITDTFNH
                     YRYIQMEQEAETDNSSFDTESAGSKLSEKAEQSITTIIHHIITGGNQHD"
     gene            complement(505..987)
                     /locus_tag="PLU43_10365"
     CDS             complement(505..987)
                     /locus_tag="PLU43_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO28856.1"
                     /translation="MITSYQHLIFTICYKFTNNYFDAEDLTQDTFLSAYKHLENFDGN
                     NPKSWLCRIATNKCLDFKKRAASGTVPTDDSFFLTIETDYASPEKEYLEVSVKKELLA
                     CCNSLSPPYAEVAREYFYNERTVSELARLLNRNEKTLRTQIYRAKGMLRKLYGKEVRS
                     "
     gene            1205..>1486
                     /locus_tag="PLU43_10370"
     CDS             1205..>1486
                     /locus_tag="PLU43_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO28857.1"
                     /translation="MTVLVTGGAGFIGSHTCVELINAGYEVVIADNFYNSCMEAVRRV
                     EKITGKSVKFYEADLLDRKALEEIFEREQIEAVIHFAAYKAVGESVEKPL"
BASE COUNT          391 a          266 c          343 g          486 t
ORIGIN      
        1 acccgcaccc ggtatcagtg aaaaaataaa agtccaaaat ccggtttttt ttctaatcat
       61 gttgattacc tccagttata atatgatgaa tgatcgtagt gattgactgc tccgcttttt
      121 cagaaagctt tgagccggcc gattctgtat caaaagagct gttatctgtt tcggcttcct
      181 gttccatttg tatatacctg taatgattga atgtgtcggt tatggaaaaa gtaaggccgg
      241 gagatatttt ctcaatggcg atcagctggc acaggcagat aatgactgct gccgatattt
      301 tgaaagtata gaacagaaac tgcatctgtt ttgaaaattc ttttttctgt ttctgcagtc
      361 tgagcgggac ctgtacgctg acacttctgc tttttataag tatttcttcc tttaaattag
      421 caggagcgaa cagcagcgta tgtgcttctg tttccctgat cagtgcttca atgccgatct
      481 cttgttctgt ctgtttggat atgctcatga gcggacctcc tttccataaa gctttcttaa
      541 cattcctttt gcccggtaga tctgtgtacg cagtgttttt tcattgcggt tcagcagccg
      601 tgcaagttca gatacggttc tttcattgta aaaatattca cgtgcgactt cggcatatgg
      661 cggtgaaaga ctgttgcagc atgcaagcag ttcttttttt acggacactt ccagatattc
      721 tttttcagga gaggcgtagt cggtctctat tgtcagaaaa aaagaatcat cggtcggaac
      781 agttccggat gccgcccgtt ttttgaaatc caggcattta tttgttgcga ttctgcaaag
      841 ccatgatttc ggattgtttc cgtcgaagtt ttcaaggtgc ttgtaagcgg acaggaatgt
      901 atcctgtgtc aggtcttcgg catcgaaata attgtttgta aacttatagc agatcgtaaa
      961 tattaagtgc tggtagcttg tgatcatatg ttcaagctgc tgctgagtat cgatgttgcc
     1021 cgcctccctt caacttgctc tacataatat aacgaggaac cgctgtatta tatttcatta
     1081 atttttaatt ttaaatgata actgctgaaa agtatgtcag gaatgtaagt tgccaattct
     1141 attattatag tgtaatatta tctggtattg caacattgga gtgatttgaa aggagcagga
     1201 cagtatgacg gttttggtaa caggcggggc tggatttatc ggcagccata catgtgtgga
     1261 gctgatcaat gccggttatg aggttgtgat tgcagataat ttctataatt cgtgcatgga
     1321 agcagtcaga agagtagaga aaattacggg aaaatcagta aaattttatg aggcggatct
     1381 tcttgacaga aaagcgcttg aagaaatatt tgaacgggaa cagatagagg cggtcattca
     1441 ttttgcggct tacaaggcag taggtgaatc agtggaaaag cctttg
//
LOCUS       DAOSYE010000666         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_77387_length_1479_cov_2.349719, whole genome shotgun sequence.
ACCESSION   DAOSYE010000666 DAOSYE010000000
VERSION     DAOSYE010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77387_length_1479_cov_2.349719"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLU43_10375"
     CDS             <1..493
                     /locus_tag="PLU43_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28858.1"
                     /translation="VTYNGFLMDFRTSKDLPEHDGYLLKDNTTLGERNVSYLKKTIEL
                     CRENGVQLMLMKVPSYSDNWSLDYDRQIEEIASQYDISYVNFDAYSDEIGLDYHKHSP
                     DEGSHLNTPGAELFTGYLGEYISKKYEVPSHAEEESYKKIWDVKMKRYEDDKKAGMEE
                     IID"
     gene            623..>1479
                     /locus_tag="PLU43_10380"
     CDS             623..>1479
                     /locus_tag="PLU43_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOO28859.1"
                     /translation="MDEKRRISLVRAEELYGEVEDFLQFKEMFLRETEERRDIISKKH
                     KEELLSYFEAEENEWEDWHWQLKNRISSVSTLKKFINLSEQQIAMFEEISKKYRWATT
                     PYYLSLMDYEDMSYPINRMALPTAEENDMAGELDPMDEEYTNPAPCITRRYPNRLIIN
                     VTSSCAMFCRHCQRRRRIGENDRIESEENINAAIAYIKDTKEIRDVLITGGDPLMLPD
                     EMLESIVRRVREIPHVEVIRIGTRVPVTMPQRITQKLADMLKKYHPLFMNFQFNHPGE
                     LTQEAVQAC"
BASE COUNT          460 a          248 c          391 g          380 t
ORIGIN      
        1 ggttacttac aacggttttt tgatggactt tcgtacgtca aaggatttgc cggaacatga
       61 cggatatctt ttgaaagata acacaacgct tggagagcgg aatgtttcct atttaaaaaa
      121 gacgattgaa ctgtgccgtg aaaacggagt ccagcttatg ctgatgaaag taccgagcta
      181 ctcggataac tggagtctgg attatgaccg gcagattgaa gagattgctt cgcaatacga
      241 tatttcttat gtgaattttg atgcatacag cgatgagatc ggactggatt atcataaaca
      301 ttcgccggat gagggcagtc atctgaatac gccgggagcg gagctgttta cgggatatct
      361 tggagagtat atttcgaaaa aatatgaggt gccgtcccat gcggaggaag aaagctataa
      421 aaaaatctgg gatgtgaaaa tgaagcgtta cgaagatgat aaaaaggccg gaatggagga
      481 aattatcgac tgagcaatgc agtgattggt ttataatcat aacgtgggtt gcctgcctgc
      541 ctaaaaaggt gtataataaa atagttgtat ttacacataa atatgttaac tattttatta
      601 atggaggttt gatgttatta taatggatga aaaaagaaga atatctcttg taagggcgga
      661 agaattatat ggtgaagttg aggatttttt acagtttaaa gagatgtttc ttcgagaaac
      721 ggaggaaaga cgggatatca tcagcaagaa gcacaaggaa gaattgctga gttatttcga
      781 agcagaggaa aatgagtggg aggactggca ctggcagttg aaaaaccgta ttagttccgt
      841 ctccacgctg aaaaaattta tcaatctttc agagcagcag attgcgatgt ttgaagagat
      901 ctcaaaaaaa taccggtggg cgacgactcc atattattta tcactgatgg attatgaaga
      961 catgtcgtat ccgatcaacc gcatggcatt gccgacagca gaagaaaatg atatggcagg
     1021 agaactggat ccgatggatg aagagtatac gaatccggcg ccctgtatta caaggcgtta
     1081 tccgaatcgt ctgattatca atgtgacaag ctcgtgcgcg atgttctgcc gtcattgcca
     1141 gcgcagaagg cggatcggtg agaatgaccg gatcgagtca gaggagaata tcaatgctgc
     1201 gattgcctat ataaaagata caaaagagat cagggatgtg ctgattacag gaggagatcc
     1261 gctgatgctg ccggatgaga tgcttgagag tattgtcagg cgcgtacgtg aaatccctca
     1321 tgtggaagtg atccgtatcg gcacgcgtgt tccggtgaca atgccgcagc gtattacgca
     1381 gaaacttgcc gatatgctga aaaagtatca tccgctgttt atgaattttc agttcaacca
     1441 tccgggagag ctgacacagg aggctgtaca agcctgctg
//
LOCUS       DAOSYE010000667         1478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_77449_length_1478_cov_2.942375, whole genome shotgun sequence.
ACCESSION   DAOSYE010000667 DAOSYE010000000
VERSION     DAOSYE010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77449_length_1478_cov_2.942375"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLU43_10385"
     CDS             complement(<1..208)
                     /locus_tag="PLU43_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28860.1"
                     /translation="MEKREVTAVKEEGLTIKDILLIGILLAAGAVLKFFVGSVINFGM
                     KPNFIIAMYCLIILLIKPRVKEAAI"
     gene            complement(635..1471)
                     /locus_tag="PLU43_10390"
     CDS             complement(635..1471)
                     /locus_tag="PLU43_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28861.1"
                     /translation="MKLSGKRNVLYCVLSILILCILLFPLYWIFVTSLKTEKEIFQIP
                     PTFWPEILNLKSYQAQLENGDFNMFRSFGNSLIISVGAMAISILLAVPASYGIAKYHF
                     KGKQAVVLGFLVTQMLPVSVLLTPLFIMFRNMHLYNTLFSTILADATIGIPFSVLILK
                     NYFASIPKDMEEAAYIDGCNRFSAFMKVLIPIAKPGVVVCGVFSFLYAWGDLAYGMTF
                     ILDQEKRPITAGIFNFMGQYGTKWSYLTAFAVVTIIPVALIFIFMQKYIISGMTSGAV
                     KG"
BASE COUNT          427 a          348 c          282 g          421 t
ORIGIN      
        1 taatcgctgc ctcttttaca cgcggtttga tcaataagat aatcagacag tacattgcaa
       61 taataaaatt cggcttcatg ccaaaattaa tcacacttcc tacaaaaaac tttaacactg
      121 cgcctgccgc aagcaaaata ccgatcaaaa gaatatcctt gatcgtaagt ccctcttcct
      181 ttactgctgt tacttctctt ttttccatga taaacatcct ttccctgagg ccctgcctca
      241 aatccataat aagattgacg gttttcatgt tggtttccat tttcgctccg gcgggtctac
      301 acaaaaaaac ccgtccttaa atatttaagg acaggcttat tatcgtctgc ggtaccacct
      361 taattgattt gcatgatcac aaatccactc tctcaaaaat gccaacacat tttctgccgg
      421 taacgtcggc ttacgtttcg ggtactaacg agtcatctgg tgactgcggg ttgcctcacc
      481 ctccgtggtc catttgccat ctcgcatctc cgcaggctct cagcaatacc tgctctctgt
      541 aggcgcgtta tatggtttga tctccacatc agcggtttga gtatttattt tgcctatcat
      601 aacattccag attttatttg tcaagaattt tttgttatcc ttttacggct ccgcttgtca
      661 tgccgctgat aatatatttc tgcataaaga taaagataag tgcaaccgga atgatcgtaa
      721 cgaccgcgaa cgcagtcagg taactccatt ttgttccgta ctggcccata aagttaaaaa
      781 ttccggcagt gatcggacgt ttttcctgat ccagtatgaa ggtcattcca tatgcaagat
      841 ctccccaggc atacaaaaaa gaaaagaccc cgcatacgac cacacccggc tttgcgatcg
      901 ggatcagcac cttcataaaa gcactgaatc gattgcagcc gtcaatatag gctgcttctt
      961 ccatatcttt tggaatcgaa gcaaaatagt ttttcaagat cagaaccgaa aacggaattc
     1021 cgatcgttgc atccgcaagt attgtggaaa ataacgtatt atacagatgc atattccgaa
     1081 acataataaa cagcggtgtc agcaggacag agaccggcag catctgcgta acaagaaagc
     1141 cgagaaccac tgcctgtttg cctttaaaat gatactttgc aattccatag gaagcgggaa
     1201 cggcaagcag gatcgatatt gccatcgcgc ctacggaaat gatcagactg ttcccgaatg
     1261 agcggaacat attgaaatcg ccgttttcca gctgtgcctg ataggatttt aaatttaata
     1321 tttccggcca gaatgtgggc ggtatctgaa atatttcttt ttctgttttc agggaagtta
     1381 caaagatcca atagagcgga aacagaagga tacataaaat aagtatggaa aggacgcaat
     1441 acaggacatt ccttttccct gacaatttta tttccgag
//
LOCUS       DAOSYE010000668         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_77614_length_1476_cov_3.011260, whole genome shotgun sequence.
ACCESSION   DAOSYE010000668 DAOSYE010000000
VERSION     DAOSYE010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77614_length_1476_cov_3.011260"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1476)
                     /locus_tag="PLU43_10395"
     CDS             complement(<1..>1476)
                     /locus_tag="PLU43_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO28862.1"
                     /translation="EGHCYLPKEVVMKRAAGLLGVSPEAVWNQAVNLSMDRKLIIKER
                     NSTIFVYAGSYYYMELNCARYLHDLNVDSEENVETVKKRLEWLVKEEGIELDQLQLSA
                     AVSAVMHGVVVITGGPGTGKTTTINLIIKYFESEGLDILLAAPTGRAAKRMTEATGYE
                     ASTIQRMLKLNPVSDSDARGYSFEKNEENPLEADAVIIDEMSMVDLPLFTALLKAVSI
                     GTRLVLVGDTNQLPSVGPGSVLKDIISSECFEVVRLKKIFRQAEQSDIVVNAHKINQG
                     EPIKLDNKSRDFFFLKREDVNVILKTIVVLVKDKLPGYVHAKPYDIQVLTPMRKGPLG
                     IETLNPILQKFLNPPDEKKEEKEYGDMIFREGDKVMQIRNNYQLEWEVRSRTGISVDR
                     GLGIFNGDMGIVKTINHYMEMMTVEYEERRQVQYPFAQLEELELAYAVTIHKSQGSEY
                     PAVIIPLLSGPRMLFNRNLLYTGITRAKECVTLIGSEEQIGR"
BASE COUNT          355 a          411 c          279 g          431 t
ORIGIN      
        1 ccgcccgatc tgttcctcac ttccgatcag tgtcacgcat tctttggcac gggtgatgcc
       61 ggtataaagc aggtttcggt taaacagcat ccgcggaccg ctgagcagcg gaatgatcac
      121 tgcggggtat tcacttccct gtgacttatg tatcgtaacc gcataggcca gttccagttc
      181 ttccagctgg gcaaacggat attggacctg tctgcgctcc tcatattcga ctgtcatcat
      241 ttccatataa tgattgatcg ttttcacaat acccatatct ccgttaaaaa taccaagccc
      301 tctgtcaacg gaaataccgg tccggcttct gacctcccat tccagctgat aattgtttct
      361 gatctgcatg actttatcgc cttcccgaaa aatcatatcc ccatattctt tttcttcctt
      421 tttttcatcc ggcggattca gaaatttctg cagaatcgga ttcagcgttt ctattccaag
      481 aggacccttg cgcatcggtg tcagcacctg aatatcatat ggctttgcat gaacatagcc
      541 cggaagctta tctttgacaa gcacgacgat cgtcttcagg atcacattga catcttctct
      601 ttttaaaaag aaaaagtccc tgcttttatt gtccagtttt ataggttctc cctgattgat
      661 tttatgtgca ttcacaacaa tatcgctttg ctctgcctgc cggaatattt ttttcagacg
      721 cacgacttca aagcactcgg aagaaatgat atcctttaga acagatcccg gaccaacaga
      781 tggcagctga ttcgtatctc ctacaagcac cagcctcgtt ccgatcgaaa ctgcttttaa
      841 cagggccgta aacaacggaa ggtctaccat tgacatctca tctataatga ctgcgtccgc
      901 ttcaagcggg ttctcctcgt tcttttcaaa cgaatatccc cttgcgtcgg aatcagatac
      961 cggattcagc ttcagcatcc gctggatcgt actcgcttca tatcctgtcg cctccgtcat
     1021 ccgttttgcc gcccttccgg tcggggcagc aagcagaata tccagtcctt ccgattcaaa
     1081 atattttata atcagattga tcgtggtcgt tttacccgtt ccaggacctc ccgtaatcac
     1141 gaccacgcca tgcataacgg cactgacagc agccgaaagc tgaagctgat caagttcaat
     1201 cccttcctcc ttcacaagcc attccagtct ctttttgacc gtttccacgt tttcttcaga
     1261 atctacattc agatcatgca gatatctggc gcagttcaat tccatataat aatagctgcc
     1321 tgcatataca aagatagtac tgttcctctc cttaatgatc agctttcggt ccatggaaag
     1381 gttgactgcc tggttccaga ctgcctccgg cgatacgcca agaagtccgg ccgcacgctt
     1441 cataacaacc tctttcggaa gataacaatg tccctc
//
LOCUS       DAOSYE010000669         1474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_77763_length_1474_cov_3.718816, whole genome shotgun sequence.
ACCESSION   DAOSYE010000669 DAOSYE010000000
VERSION     DAOSYE010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77763_length_1474_cov_3.718816"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="aspS"
                     /locus_tag="PLU43_10400"
     CDS             <1..1387
                     /gene="aspS"
                     /locus_tag="PLU43_10400"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOO28863.1"
                     /translation="FSKYCMGTVLTNPEVKNKIVLRSKIVAELRQKMMDLDFLEITTP
                     ILTCSSPEGARDYLVPSRVHPGKFYALPQAPQQFKQILMASGFDRYFQIAPCFRDEDA
                     RADRSPGEFYQFDMEMAFATQDDVFSVIEEVLPPVFEKYGVYKTNSVTPFTRIPYLEA
                     MEKYGSDKPDLRIDLTVQDATDCLAGCRFEPFDGNEIKAVVVDGFNASRKIIDKICED
                     VFIRTGDKAYWFKLDDKNEFVGGISKFLADRKEQVISSLNLKPGDFIGLTAGKKIKAQ
                     KAAGVLRNLLGAGCEAHMKKDVYQFCWIVDFPMYEIGEESGKLEFCHNPFSMPQGGME
                     ALTSQDPLDILAYQYDLVCNGVELSSGAVRNHDPEIMVKAFQLIGLGEADVKTKFPAM
                     YNAFCYGAPPHAGIAPGIDRMVMLITGEESIKEVIPFPMNKSAADIMMNAPAKVEKKQ
                     LDDVHIRIIEE"
BASE COUNT          413 a          296 c          387 g          378 t
ORIGIN      
        1 tttcagcaaa tattgtatgg gtaccgtgct cacaaatccg gaagtaaaaa ataaaatcgt
       61 gctgcgaagt aagatcgttg ccgaattaag acagaagatg atggatttgg attttctgga
      121 aatcacgacg ccgatcctga cctgttcttc gccggaaggc gcaagggatt atctcgttcc
      181 ttccagggtg catccaggaa agttttatgc actgccgcag gcgccccagc agttcaaaca
      241 aattctgatg gcatccggtt ttgatcgtta ttttcagatc gccccctgtt ttcgggatga
      301 ggatgcgcgt gccgaccgtt ctccggggga gttttatcag tttgatatgg aaatggcatt
      361 tgcaacacag gatgatgttt tctctgttat tgaagaggtc cttccgccgg tttttgagaa
      421 gtacggtgtt tataagacga attctgtcac gccgtttacc cgtattcctt atttggaggc
      481 aatggaaaag tacggatccg acaagcctga ccttcgcatt gacttaacgg ttcaggatgc
      541 aacggactgt cttgccggct gcagattcga accatttgac gggaatgaaa taaaagccgt
      601 agtcgttgat gggttcaatg ccagccggaa aatcatcgat aaaatctgtg aggatgtatt
      661 tatacggacc ggcgataaag catactggtt caaattggat gataaaaatg aatttgtcgg
      721 aggaatcagc aaatttttgg cagacaggaa agagcaggta atttcttcct tgaacctaaa
      781 gccaggagat tttatcggcc tgactgccgg taaaaaaata aaagcacaaa aagctgcggg
      841 tgtgcttaga aatctgcttg gagcgggatg tgaagcacat atgaagaagg acgtgtatca
      901 gttctgctgg atcgttgatt ttccgatgta tgaaatcgga gaagaatccg ggaaactgga
      961 attttgccat aatccgtttt ctatgccgca gggaggcatg gaggcactga ccagtcagga
     1021 tccgttggat atactggcct atcagtatga tcttgtctgc aacggtgtcg aattatcctc
     1081 cggggcagta cgtaatcacg atccggagat catggtaaaa gcatttcagc tgatcggact
     1141 gggggaagcg gatgtaaaaa caaaatttcc cgccatgtac aatgcgttct gctatggagc
     1201 gccgccgcat gccgggatcg caccgggtat cgatcgtatg gtcatgctga tcacagggga
     1261 agagagtatt aaagaagtca ttccgttccc gatgaataaa agcgcggcag atattatgat
     1321 gaatgcacct gccaaagtag agaaaaaaca gctggatgac gttcatatcc ggatcataga
     1381 agaataatca gcaaaatcag acagagggca tgccctctgt ttttgtttac ggcttgagcc
     1441 tggctgagct cgcaaagcgt gcgaagtcaa aaaa
//
LOCUS       DAOSYE010000670         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_77859_length_1473_cov_3.248237, whole genome shotgun sequence.
ACCESSION   DAOSYE010000670 DAOSYE010000000
VERSION     DAOSYE010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77859_length_1473_cov_3.248237"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLU43_10405"
     CDS             <1..722
                     /locus_tag="PLU43_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOO28864.1"
                     /translation="AVCGDLVDVVTLVPPGNSPENYEPTAQEMEEFSDASLYFSIGVP
                     TEEANIMPNVGDVKVISLADAVAAVYEDRTFDSGERDPHIWLSPKRVKVMIDTIAEEV
                     GKIDPDNQETYNENAESYKAKLDEVDQEIKDALEGVENKKFIVYHPAFGYIADDYGLE
                     MFSLEEEGKEATAQHLQDMIDLAKEENIKVIFYQEEIDSSQSEAFAEELGGKTIQLAP
                     LAADYIDNLKKMAQTMAEAMQ"
     gene            722..>1473
                     /locus_tag="PLU43_10410"
     CDS             722..>1473
                     /locus_tag="PLU43_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28865.1"
                     /translation="METVIHTDDLSVYYGDTAAITNICLTVEKGEFLGIIGPNGGGKS
                     TLLKAILGLVPVTAGNILVYGQAVKKSRIAVGYVPQFATMDRRFPISVLEVVLTGRLK
                     AGLSLFAKYSGEDREVAYTQLKRVGIEHLAKRQISELSGGEFQKLLIARALMVHPALL
                     LLDEPTASVDVKSREQIYHLLKELNDEMTIVLVTHDLLAISSEVKTLACLNRRLVYHG
                     EPQLDEQIVRELYGCPVDLIAHGVPHRMLKKH"
BASE COUNT          427 a          284 c          419 g          343 t
ORIGIN      
        1 aagctgtctg cggagatctg gtcgatgttg ttacacttgt tcctcctgga aacagcccgg
       61 agaattatga gccgacggca caggaaatgg aggagttcag tgatgcgtca ctttattttt
      121 cgatcggcgt gcctacggag gaagcaaata tcatgccgaa tgtaggagat gtcaaagtca
      181 tatcactggc ggatgcggtt gctgccgtat atgaagaccg gacgtttgat tcgggagaaa
      241 gagatccgca catatggctt tcaccaaagc gtgtcaaggt aatgatagac acgattgccg
      301 aggaagtcgg taaaatagat ccggataatc aggaaactta caatgaaaat gcggaaagct
      361 ataaagcaaa gctggacgag gtggatcagg agatcaaaga tgccctggag ggtgttgaga
      421 ataagaaatt catcgtatat catccggcgt tcgggtatat cgcagatgat tacgggctgg
      481 aaatgttttc tctggaagaa gagggaaaag aggcaacagc ccagcattta caagacatga
      541 tcgatcttgc gaaagaggag aatatcaaag tgatttttta tcaggaagag atcgacagca
      601 gccagtctga ggcatttgcc gaagaattag gcggaaaaac gatacagctt gcgccgcttg
      661 cagcagatta tatcgataat ctgaagaaaa tggcgcagac gatggcggag gctatgcaat
      721 aatggaaacg gtgatccata ctgatgatct gtccgtatat tatggagata cggctgccat
      781 aacgaatatc tgccttactg ttgaaaaggg agagttcctt ggcattatcg gaccaaacgg
      841 cggagggaag tccactttat taaaagctat tcttggtctg gtgccggtta cggcaggaaa
      901 tatactggtt tacggacaag cggtcaaaaa gagcaggatc gccgttggat atgttccgca
      961 atttgcaaca atggacaggc ggtttccgat ctcggtgctg gaagtcgttc tgacaggaag
     1021 gctgaaagca ggcctgtccc tgtttgcaaa atattcgggg gaagacagag aggttgcgta
     1081 tacccagcta aagcgcgttg gaatcgaaca tctggccaaa cggcagatat cggaattatc
     1141 cggcggcgaa tttcagaagc ttctgattgc gagggcgctg atggttcatc cggcattatt
     1201 gctgctggat gaaccaacgg caagcgtaga tgtgaaatcc agggaacaga tctatcacct
     1261 gctgaaagaa ttgaacgatg agatgacgat cgttcttgtt acacacgatc tgctggccat
     1321 ttcatcggaa gtgaaaacac ttgcatgtct gaacagaagg ctggtctatc atggggaacc
     1381 gcagttggat gaacagatcg tcagggagct ttatggctgt ccggttgatc tgattgcgca
     1441 cggcgttccg cacaggatgc tgaaaaagca tga
//
LOCUS       DAOSYE010000671         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78318_length_1468_cov_2.516631, whole genome shotgun sequence.
ACCESSION   DAOSYE010000671 DAOSYE010000000
VERSION     DAOSYE010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78318_length_1468_cov_2.516631"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLU43_10415"
     CDS             <1..322
                     /locus_tag="PLU43_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28866.1"
                     /translation="TVMKRILASGEEFTAVYAISDTLAIGACKAILESGRRIPEDISV
                     AGFDGIDMSYYYSPTITTIQQPFEAMAEETVAVLFNVLRKKEEHQHRIFEAKLCVGGS
                     TRKL"
     gene            complement(340..792)
                     /locus_tag="PLU43_10420"
     CDS             complement(340..792)
                     /locus_tag="PLU43_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOO28867.1"
                     /translation="MEIERKFLVKEQEMPQNLKSYPCHLIEQAYLCTEPVIRVRRENN
                     NYYMTYKSKGLMVREEYNLPLNKEAYESLKQKADGNIIGKKRVLIPYGDHKIELDIFD
                     EPFAPLIIAEVEFETVEDANAFTPPGWFGEDVTNKTEYHNSNLSRQKL"
     gene            955..1215
                     /locus_tag="PLU43_10425"
     CDS             955..1215
                     /locus_tag="PLU43_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28868.1"
                     /translation="MQAVTMTVSSVITKGKKRFVRVSFTRGTDFAEGTVPDNHIERAK
                     GFTEEEIIKLEEYLASSQTDIMERAGKINPLKSWMKETIPDT"
     gene            1324..>1468
                     /locus_tag="PLU43_10430"
     CDS             1324..>1468
                     /locus_tag="PLU43_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family protein"
                     /protein_id="HOO28869.1"
                     /translation="MKTCYIFLAEGFEEVEALTVVDLLRRDRLNVEMVSIADTDTVTG
                     SHGI"
BASE COUNT          390 a          274 c          372 g          432 t
ORIGIN      
        1 tacggtcatg aagcgtattc ttgcatcagg agaggagttc acagcagtct atgcaatttc
       61 ggatacactt gccattggcg catgcaaagc gatcttggag tccggcagac ggatcccgga
      121 ggatatttcc gtagcaggtt ttgacggaat cgatatgtct tactattaca gtccgacgat
      181 cacaacgatc cagcagccgt ttgaggcgat ggcagaagaa acggtggcgg tgctttttaa
      241 cgtgctccgt aagaaagagg agcatcagca caggatcttt gaggcaaagc tgtgtgtcgg
      301 aggttcgaca aggaaattat agagtgaata tcctgtctgt cataatttct ggcggctcag
      361 attgctgtta tggtattccg tcttatttgt gacgtcttct ccgaaccagc cgggcggcgt
      421 aaaggcattc gcgtcttcta cggtctcaaa ttctacttcg gcgatgatca ggggcgcaaa
      481 cggctcatca aaaatatcaa gctctatttt atgatctcca tatgggatca ggactctttt
      541 tttgccgatg atatttccgt cggctttttg ttttaatgat tcataggcct ctttattgag
      601 aggcagatta tattcctccc tgaccatcag gccttttgat ttataggtca tataataatt
      661 gttgttttcc ctgcgtacac ggatgaccgg ttctgtgcat aaataggcct gttcgatcag
      721 atggcacgga tagcttttca gattttgcgg catttcctgt tcttttacaa ggaattttct
      781 ttctatttcc atattgtgtg ttcctttctt ttttcagctc ctggaaggtc tgtacggata
      841 ctttcagtat aacatgaggg aggacagttt agtaaagtta atttccggcc gatataaaca
      901 aaagaagctg gttgttcaaa cagatacgaa agagtatgga acggaggcgg tgggatgcag
      961 gctgttacta tgacggtttc ttctgtcatt acaaaaggga aaaaacgatt tgtccgcgtt
     1021 tcctttacac gcggaactga ttttgcagaa gggactgttc cggacaatca tatcgaaaga
     1081 gcaaaggggt tcacagaaga ggaaattata aaactggaag aatatcttgc ttccagtcaa
     1141 acggatatta tggaaagagc ggggaagatc aatccgctga aaagctggat gaaggaaacg
     1201 attccggata cttaggaaaa ctcaaatcaa cagcagggcg tttttcattt ccctgaaaat
     1261 atggtatact gtgattgaca aggaatacgg ttgtttacat agtttacgca aaggagggtt
     1321 ttgatgaaga cgtgttatat ttttcttgcg gaaggatttg aagaagtcga ggcgctgaca
     1381 gtcgtggatc tgcttagaag agaccggctt aatgtcgaga tggtttcgat tgcggacacg
     1441 gatacggtaa cgggttcgca tggtatta
//
LOCUS       DAOSYE010000672         1467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78380_length_1467_cov_3.488669, whole genome shotgun sequence.
ACCESSION   DAOSYE010000672 DAOSYE010000000
VERSION     DAOSYE010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78380_length_1467_cov_3.488669"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1467)
                     /locus_tag="PLU43_10435"
     CDS             complement(209..>1467)
                     /locus_tag="PLU43_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO28870.1"
                     /translation="GPERKQRLELAWRTKETNEFGLNEFAKWIKKVDSEVMMAVNLGT
                     RGLSDALNLLEYCNMKEGTYYSNMRRSHGQNDPYNIKMWCLGNEMDGPWQVGHKRAGE
                     YGYLAAETGKAMKLIDDSIELTVCGSSYEKMPTFPQWEASVLDEAYEYADYISMHQYY
                     GNLENDTADYFALTQDMENFIHTVIATCDYVKAKKRSKKQMNISFDEWNVWFHSFDTD
                     NDTMKNRPWQTAPHLLEDIYTFEDAVMVGLMLITLLKHADRIKVACLAQLVNVIAPIM
                     TENDGRIFKQTIYYPFLHASKYGRGSVLETVISTSTHDTKNHEDVTDIEAVAVYREET
                     KEITIFAVNRNTADEIEFEADLRSFDRAEILEYLAMENADMKAENSFIKEAVKPYAKK
                     EYVLDDGCFSTHLKPSSWNVIRFRCK"
BASE COUNT          359 a          370 c          260 g          478 t
ORIGIN      
        1 acgacgcagg gattttttct ttttgataag atatcttccg tcaaaaggat attccagctc
       61 tttcatatga aaacaatacc tctctgtata tttaaacctt tttaagtata actgttttat
      121 aaactgattg caaatagaaa aacactgcgg acgggcaagc cggcagcagc gctttcttcc
      181 tattataaag tatccttgtt acggatgttt atttacagcg aaatctgatc acattccagg
      241 aggacggttt taaatgcgtg ctaaaacagc cgtcgtccaa tacgtattct ttctttgcat
      301 atggttttac cgcttctttt ataaacgaat tttcagcttt catatccgca ttttccattg
      361 ccagatactc caaaatctct gccctgtcaa agctgcgcag atctgcctca aattcaattt
      421 catcagctgt gtttctgttt accgcaaata tcgtgatttc cttcgtttcc tcccgatata
      481 cggcaactgc ttcgatatcg gtaacatctt catggttttt cgtatcatgc gttgaagtgg
      541 aaattaccgt ttccagcaca cttcctcttc cgtattttga cgcatgcaga aacgggtaat
      601 aaatcgtctg cttaaagatc ctgccgtcat tttccgtcat gatcggggca atcacattca
      661 cgagctgtgc aaggcaggct actttgatcc ggtcggcatg ctttaaaaga gtgatcagca
      721 tcagtccgac catcacggca tcctcgaacg tataaatatc ctccagtaaa tgcggtgctg
      781 tctgccatgg ccggtttttc atcgtatcat tgtccgtatc aaacgaatga aaccagacat
      841 tccattcatc aaagctgata ttcatctgct ttttgcttcg tttttttgcc ttgacataat
      901 cgcaggtcgc aatcaccgta tggataaaat tctccatatc ctgcgtcagc gcaaaataat
      961 ctgccgtgtc attttccaga ttgccataat actggtgcat ggaaatataa tcagcatact
     1021 catatgcctc atcgagcaca gatgcttccc actgtggaaa tgtcggcatc ttttcatagg
     1081 aactgccgca cacggtcaat tcaatactgt cgtcgatcag cttcattgcc tttcccgtct
     1141 ccgcagccag atagccgtat tcgcccgcac gtttatgtcc gacctgccat ggtccgtcca
     1201 tttcattccc aagacaccac attttaatgt tgtaaggatc gttctgtccg tggctcctgc
     1261 gcatgttgct gtaataggtt ccttctttca tattacagta ttccagcaga ttcagtgcat
     1321 cgcttaagcc tctcgttccc aaattgactg ccatcataac ttcagaatct acttttttta
     1381 tccattttgc aaactcattc aatccgaatt cattcgtttc ttttgtccgc caggcaagct
     1441 ccagtctttg ttttctctcc ggtccga
//
LOCUS       DAOSYE010000673         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78565_length_1465_cov_3.417021, whole genome shotgun sequence.
ACCESSION   DAOSYE010000673 DAOSYE010000000
VERSION     DAOSYE010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78565_length_1465_cov_3.417021"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="metK"
                     /locus_tag="PLU43_10440"
     CDS             <1..1078
                     /gene="metK"
                     /locus_tag="PLU43_10440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO28871.1"
                     /translation="SRVACETATCTGFVLVTGEITTNAYVDIQKIVRDTVKEIGYTKS
                     EYGFDGNTCAVLTAIDEQSSDIAMGVDKALEAKENKMTDEEIEAIGAGDQGMMFGYAT
                     NETEEYMPYSISLAHKLAKQLTKVRKDGILTYLRPDGKSQVSVEYDDDGKPLRLEAVV
                     LSTQHDEDVSQEQIHEDIKKYVFEPILPKELIDEDTKFFINPTGRFVIGGPHGDAGLT
                     GRKIIVDTYGGFARHGGGAFSGKDCTKVDRSAAYAARYVAKNIVAAGLAEKCEIQLSY
                     AIGVAQPTSIMADTFGTGKLSDERLVEIVRENFDLRPAGIIKMLDLRRPLYRQTAAYG
                     HFGRNDLNLPWEALDKVDILKKYL"
     gene            1204..>1465
                     /locus_tag="PLU43_10445"
     CDS             1204..>1465
                     /locus_tag="PLU43_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO28872.1"
                     /translation="MAFTHLHTHTEYSLLDGSNKIKEYVQRVKELGMDAAAITDHGVM
                     YGVIDFYRAAINAGIKPIIGCEVYVAPNSRFDKELTGGEDRYY"
BASE COUNT          420 a          296 c          392 g          357 t
ORIGIN      
        1 gagccgcgta gcctgcgaaa ctgctacctg tacaggcttt gtgcttgtga caggcgaaat
       61 tacgacgaat gcctatgtcg atatccagaa gatcgttcgt gatactgtaa aagagatcgg
      121 ttatacgaaa tctgaatatg ggtttgatgg aaatacatgc gccgtactga ctgcgatcga
      181 tgagcagtca tccgatattg caatgggtgt ggataaggca ctggaagcaa aagagaacaa
      241 aatgacggac gaggagatcg aagcgatcgg cgccggagat cagggcatga tgtttggcta
      301 tgcgacgaat gagacggaag aatacatgcc gtattccatt tcgcttgcac ataagcttgc
      361 aaagcagctg actaaagtgc gtaaggacgg tattctgacg tatttgagac cggatggaaa
      421 aagtcaggta tccgtagagt atgacgatga cgggaaaccg ctccgtctgg aggcagtcgt
      481 attatctaca cagcacgatg aggatgtatc gcaggaacag atccatgaag acattaagaa
      541 atatgtattc gaaccgatcc ttccaaagga gctgatcgat gaagatacaa agttctttat
      601 taacccgacc ggccgttttg taatcggcgg accgcatggg gatgcaggtc ttacaggacg
      661 aaaaattatt gtggacacat acggcggatt tgcccgtcac ggcggcggcg ctttttccgg
      721 aaaggactgt acaaaggtag accggtctgc ggcttacgcg gccaggtatg ttgctaaaaa
      781 tattgtggcg gcaggacttg cggaaaaatg tgaaatacag ctttcttatg cgatcggtgt
      841 tgcacagcct acttctatta tggcagatac gttcggcacc ggaaagcttt ccgatgaaag
      901 acttgtggaa atcgtacgtg aaaactttga tctgcgtccg gccggtatta tcaagatgct
      961 tgatctgcgt cgcccgcttt acaggcagac agcagcctac ggccactttg gacgtaatga
     1021 tttaaatctt ccgtgggaag cgcttgataa agtagatata ctgaaaaagt atttataatt
     1081 gagagaaata tataaaggcg gaccgtgtgt ccgtcttttg ttttgatgca aaatatgcta
     1141 tactatatgg tacagcgtca gctgaaaaag aaactgtcag cgctcaaatg aaaggaaact
     1201 gtcgtggcat ttactcatct gcatactcat acggagtaca gtcttttgga cggctccaat
     1261 aaaataaaag aatacgtaca gcgcgtaaaa gagcttggaa tggacgcggc cgcaatcacc
     1321 gatcatggcg tgatgtatgg cgtgatcgat ttttaccgcg cggcgataaa tgccggtatt
     1381 aaaccaatca ttggctgcga agtctatgtg gcgcccaatt cccgctttga taaggagctt
     1441 acaggcggag aggaccgcta ttatc
//
LOCUS       DAOSYE010000674         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78598_length_1465_cov_2.505674, whole genome shotgun sequence.
ACCESSION   DAOSYE010000674 DAOSYE010000000
VERSION     DAOSYE010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78598_length_1465_cov_2.505674"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLU43_10450"
     CDS             complement(<1..640)
                     /locus_tag="PLU43_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28873.1"
                     /translation="MKKNTIKTAAVLLAVTMAMSLTACGAAETADTSSADVEEMPVET
                     ETEAAEETSAATEEPAEAAEDATEEVAEVQQESGDDYPGFGVYYRENDPQSDSSAEIT
                     STDTITWTVANCTTEDNPQAVALAALAEELSEKTNGNFEWDIFYNSELGSESEAVELC
                     RNNTVQFVTSNVTTMPTYVERIGVFALPYLFHSEEDELTYLATSAHAYDMWTE"
     gene            complement(704..>1465)
                     /locus_tag="PLU43_10455"
     CDS             complement(704..>1465)
                     /locus_tag="PLU43_10455"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO28874.1"
                     /translation="GPGIIIAICLGIFVFVIAKKRKYKVHPKCSLRERFQWFRKSFWA
                     LLMIVIILGGISCGIFTPTEASCIAIAYCIIIVCFVYREMPFSNILNCMGETVTGIVP
                     ALAIVSSASLFGWVLQFEHLGKLMLNGMLSLTENKWIILLLIDVILLFFGMILDSTPT
                     ILLLVPIFQPLVQQLGISEIHLGIVVILNLMIGLMTPPYGQSLFMLAGATKTPFNKVV
                     SYVLPWLLPLSAALMLITFFPQICLFLPRLLGMDV"
BASE COUNT          433 a          350 c          279 g          403 t
ORIGIN      
        1 gctctgtcca catatcatat gcatgtgcgg atgttgccag ataagtcagt tcatcttctt
       61 cactatggaa cagataaggc aatgcaaata caccgatcct ctccacatat gtcggcattg
      121 ttgttacatt tgaagtaacg aactgaaccg tattattacg gcagagttca acagcttctg
      181 actctgaccc gagttcggaa ttatagaaaa tatcccattc aaaattacca tttgtctttt
      241 cagacaattc ttccgcaagt gcagcgagag cgactgcctg tgggttatct tccgtcgtac
      301 agtttgcaac tgtccatgtg atcgtatctg ttgaggtgat ctcagccgaa ctgtcagact
      361 gcggatcatt ctccctgtag tatacgccaa atccaggata atcatctcct gattcctgct
      421 gtacttcggc aacttcttcc gtagcatctt ctgctgcttc cgcaggttct tccgtggctg
      481 cagacgtctc ttcagccgct tctgtttctg tttccaccgg catttcttcc acatctgcag
      541 atgatgtatc cgcagtctct gcagcaccgc aggctgtcag cgacattgcc attgtaactg
      601 ccagcagaac tgctgccgtt tttatcgtat tctttttcat ttttaataat ctcctttcaa
      661 actatacatt tttttatttt tatactgttg caacaatatc atattaaaca tccattccaa
      721 gaagtctcgg caggaacaga caaatctgag gaaaaaaggt gatcagcatt aatgcggcag
      781 acaatggaag caaccacgga agtacatagc tgacaacttt attaaatgga gtttttgtag
      841 caccggcaag cataaagagc gactgcccgt aaggtggtgt cattaatcca atcatcagat
      901 tcagaatcac gacgattcca agatggatct ccgaaatacc aagctgctgg acaagcggct
      961 gaaaaatcgg aacaagaagc aggatcgtcg gtgtggaatc caggatcatt ccaaaaaaca
     1021 gcaggatcac gtcgatcagc agtaaaatga tccatttgtt ttctgtcagg gagagcattc
     1081 cattcagcat cagctttccc agatgctcaa actgcagaac ccagccaaat aatgatgcgg
     1141 atgatacaat tgccagtgca ggcacgattc cagtcacagt ttctcccata caattcaaaa
     1201 tatttgaaaa tggcatttca cgataaacaa aacagacaat aataatacaa taggcaattg
     1261 caatacagga agcttccgtt ggagtgaaaa taccgcatga aatgccaccc aatataatta
     1321 cgatcatcaa caatgcccaa aaactctttc gaaaccactg aaaccgttcc cttaacgaac
     1381 attttggatg aactttatac tttctcttct ttgcaatcac aaatacaaag attccaaggc
     1441 agatcgcaat gataatgccg gggcc
//
LOCUS       DAOSYE010000675         1464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78680_length_1464_cov_2.462740, whole genome shotgun sequence.
ACCESSION   DAOSYE010000675 DAOSYE010000000
VERSION     DAOSYE010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78680_length_1464_cov_2.462740"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLU43_10460"
     CDS             <1..740
                     /locus_tag="PLU43_10460"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO28875.1"
                     /translation="YTAGAIASIAYGIKRPAVDGNVLRVITRICADETDIAKQSFKKK
                     IENTLFEVMPADRPGIYNQALMELGALICVPNGPAHCAVCPVSAFCEAYRQGRTEDFP
                     VKTPLKARTIEKKTVFLIKDGDSFALNKRGNHGLLAGFYEFPNQEGYLSETEAVAYLK
                     KRGFDAMRVEKLPFAKHIFTHKEWHMAGYLVRIAQRQDPSEKRRMQGNSGSGAEYVFA
                     TVGEMEAVYPVPSAFAAYKKQLGYLKH"
     gene            783..>1464
                     /locus_tag="PLU43_10465"
     CDS             783..>1464
                     /locus_tag="PLU43_10465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28876.1"
                     /translation="MKLLSVAIPCYNSENYMRKAIECLLPGGEDVEVIVVDDGSKDGT
                     AAIADEYAAKYPKIVKAVHQENGGHGEAVNAGLRNATGLYYKVLDSDDWFQEEAYRKV
                     LDKLREITGGQKTLDMLICNFVYEKEEKKRKKVMTYRAVLPQNEIFTWHDIKFFLPGQ
                     YILMHSVIYRTKLLRECGLELPKHTFYVDNIFVFQPLPYVKTMYYMDVNLYRYYIGRE
                     DQSVNEQIM"
BASE COUNT          442 a          236 c          402 g          384 t
ORIGIN      
        1 cgtatactgc aggggcgatc gcttccatag cgtatggaat aaagagaccg gcagttgacg
       61 gcaatgtttt acgtgtcatt acaagaatct gtgcggatga aactgatatt gcgaaacagt
      121 cgtttaagaa aaaaattgaa aatactctgt ttgaggtgat gcctgcagat aggccgggga
      181 tctataatca ggcattgatg gagcttgggg cattgatctg tgttccgaat ggtccggctc
      241 attgtgcggt ctgtcccgtg tcagcttttt gtgaagcgta caggcagggg cggacggagg
      301 attttcctgt aaaaacaccg ttaaaagcgc gcactattga aaaaaagacg gttttcctga
      361 ttaaggacgg agacagcttt gctttgaata agcgcgggaa tcatgggctg cttgcagggt
      421 tttatgaatt tccaaatcag gaaggatatc tgagtgaaac agaggcagtt gcgtacttga
      481 agaaacgggg atttgatgcc atgcgtgtgg aaaagcttcc ttttgcaaag catattttta
      541 cacataagga atggcatatg gccggttatc ttgttcgaat agcacagaga caggatccgt
      601 cagaaaaaag gaggatgcag ggaaacagcg ggagcggcgc ggagtatgtg tttgcaaccg
      661 taggagagat ggaggcggtt tatccggtgc cgtcggcgtt tgcggcatat aaaaaacagc
      721 ttgggtattt gaaacattga gaagaaaaat aattaaattt agagataaaa ggagcagaac
      781 agatgaaatt attatccgtt gcgatacctt gttataattc tgagaactat atgagaaagg
      841 cgatcgaatg tcttttgccg ggaggagaag atgttgaggt cattgttgtg gacgatggtt
      901 caaaagatgg aacggcagct attgcggatg aatatgcggc gaaatatccg aagattgtaa
      961 aggccgtgca tcaggagaac ggcggacacg gtgaagcagt caatgcaggg ctgcgtaatg
     1021 ctacgggact ttattataaa gtgcttgaca gtgacgactg gtttcaggaa gaagcataca
     1081 ggaaggtact tgataagctg cgtgaaatta caggcgggca gaaaacactg gatatgctga
     1141 tctgcaattt tgtttatgaa aaagaagaaa aaaagcgtaa aaaggttatg acttatcgcg
     1201 cagtgcttcc gcagaatgag atctttacat ggcatgatat aaaattcttt cttccagggc
     1261 agtatatttt gatgcattcg gttatttacc ggacaaaact tttacgggaa tgcggactgg
     1321 aacttccgaa gcatacattt tatgtagata atatttttgt attccagccg ctgccctatg
     1381 tgaaaacaat gtattatatg gatgtgaacc tgtaccgcta ttatatcggc agggaagatc
     1441 agtctgtaaa tgaacagatt atga
//
LOCUS       DAOSYE010000676         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_78764_length_1463_cov_2.789062, whole genome shotgun sequence.
ACCESSION   DAOSYE010000676 DAOSYE010000000
VERSION     DAOSYE010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78764_length_1463_cov_2.789062"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLU43_10470"
     CDS             <1..1133
                     /locus_tag="PLU43_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOO28877.1"
                     /translation="LGSLIYVGVCALIHILDPFIDGSAYLKMKHEMLQEKEEERKGRY
                     GLAVFFGILILIPLLIIVLRLLSSADPVFGSMLERLYINMYVPEYIFGIAGLTLFAFF
                     AAYGCFASVMENTVSGKDTCLKKGEPVMAITFSSVLAAVYFVFSLIQIIYLFGGRGAL
                     PAGYSYAQYARQGFFQLLFVCILNLGLVLVCLTCFGESKLLKIILTIISGCTFIMIAS
                     SAYRMLLYIDVYKLTFIRVLVLWSLLLLAVLLAGILVSIYHMRFPLFKYCMLAVTLLY
                     LGLSFSHQDYWIAKYNISAAGKENTQGDWNYISCLSKDAIPALAEYEKSGKAAVSAEE
                     NYFLCYYESVRDDLITEGERMTFRTFNLSKWMAWKTAAASRD"
     gene            complement(1138..>1463)
                     /locus_tag="PLU43_10475"
     CDS             complement(1138..>1463)
                     /locus_tag="PLU43_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO28878.1"
                     /translation="SSDSTLALKTGITFLRIVSPFYLIICLKLMADGVLRGAGAVKAF
                     MATTFSDLILRVILSYILSVPFGVNGIWYSWPIGWLTATVLSLYFYMTGAWKIKRPVD
                     EFEEP"
BASE COUNT          379 a          290 c          371 g          423 t
ORIGIN      
        1 atctgggctc gcttatttat gtgggtgtct gtgcactcat tcatattctg gatccgttta
       61 ttgacggcag cgcgtattta aaaatgaaac atgaaatgct gcaggaaaaa gaagaggaga
      121 gaaaggggag gtacggactt gccgtctttt tcgggatcct gatcctgatt ccgctgctga
      181 tcatcgtatt gcggctgctt tcttcggcag atcccgtgtt tggaagtatg ctggagcgac
      241 tctatataaa tatgtatgtt ccggaatata tttttggaat cgcagggctg acactgttcg
      301 cgttttttgc agcttacggc tgttttgctt ccgttatgga aaatacggtt tccggtaaag
      361 acacctgtct gaaaaaggga gaaccggtca tggcgatcac gttttcttct gttcttgccg
      421 ctgtttattt cgtgttcagt ctgatacaga tcatttatct gtttggaggc agaggggcgc
      481 ttccggcggg gtattcctat gcacaatatg caagacaggg cttttttcag ctgctgtttg
      541 tctgtatact caatcttggt ctggtgcttg tctgcctcac ctgttttgga gaaagcaaat
      601 tattgaaaat aattttaaca atcatttcag gctgtacatt tattatgatc gcatccagtg
      661 catatcgtat gttattatat atagatgtct ataaactgac atttatcaga gtgcttgtat
      721 tatggagtct gcttctgctt gcggtgctgc ttgccggaat ccttgtcagc atttatcata
      781 tgcgctttcc gttgttcaaa tactgtatgc tggccgttac actgctgtat ttgggattat
      841 cgttcagcca tcaggactac tggatcgcaa agtataatat cagcgctgcc ggaaaggaaa
      901 atacacaggg cgactggaat tatatcagct gtttatcaaa ggatgccata ccggcgctgg
      961 cagaatacga aaagagcggg aaagcggccg tgagcgcgga agaaaattat tttctatgct
     1021 attatgagag tgtaagggat gacctgatca cagagggaga gcgcatgacg ttccgtacgt
     1081 ttaatctttc aaaatggatg gcatggaaga cagcggctgc gtcgcgggac tgaaatgtta
     1141 tggttcttcg aattcgtcca caggcctttt tattttccat gcgcctgtca tataaaaata
     1201 tagagaaagt acggtggcgg taagccagcc gatcggccag gaataccaga tcccgttgac
     1261 gccaaatggt acggaaagaa tataggataa aataacgcgc aatattaaat cggaaaaggt
     1321 cgtggccatg aaagctttta cagcgccggc tccgcgcaga actccgtctg ccatcagctt
     1381 caggcagatg atcagataaa aaggagaaac gatacgcaga aaagtgatgc cggtcttcag
     1441 cgcaagggta ctgtccgaag aca
//
LOCUS       DAOSYE010000677         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79069_length_1460_cov_2.136655, whole genome shotgun sequence.
ACCESSION   DAOSYE010000677 DAOSYE010000000
VERSION     DAOSYE010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79069_length_1460_cov_2.136655"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1460
                     /gene="dnaE"
                     /locus_tag="PLU43_10480"
     CDS             <1..>1460
                     /gene="dnaE"
                     /locus_tag="PLU43_10480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOO28879.1"
                     /translation="LVLLAENNTGYKNLMKIVSRGFTEGFYYRPRVDMEILREYHEGI
                     IALSACLAGEVQRYIQKGLYDEAKKMALKYENCFGKGNYFLELQDHGIPEQKTVNAAL
                     LRMSRELAIPLVATNDIHYTYAQDAAPHDILLCLQTGKKLQDENRMRYEGGQYYVKSE
                     EEMKKLFPYALGALENTHKIAERCHVEIEFGKYKLPKFDVPEGYSAWEYLNKLCFEGL
                     DKRYPGYGKDMEERLRYELDIIHSMGFVDYFLIVWDFIRFAKSSGIAVGPGRGSAAGS
                     VVSYSLEITDIDPMKYNLLFERFLNPERVSMPDIDIDFCFERRQEVIDYVVEKYGKDK
                     VVQIVTFGTLAAKGVIRDVGRVMDLSYAYVDSIAKMVPNELNITLERALELNQDLKKL
                     YETDEQVKTLIDMCKRLEGLPRHTSMHAAGVVICQEAADEFVPLSRASDGSITTQFVM
                     TTLEELGLLKMDFLGLRTLTVIQNAVHLVEQDTGVK"
BASE COUNT          407 a          258 c          403 g          392 t
ORIGIN      
        1 tctcgttctg cttgcggaga ataatacggg ttacaaaaat ctgatgaaaa tcgtcagcag
       61 gggatttacg gaaggctttt actaccgccc gcgtgttgat atggaaatac tgcgtgaata
      121 tcacgagggt attatcgcac tttcggcttg tctggccgga gaggtacaac ggtatatcca
      181 aaaaggactt tatgacgaag caaaaaaaat ggctctgaaa tatgagaact gttttggaaa
      241 gggcaattat tttctggaac tgcaggatca tggaataccg gagcagaaaa cagtcaatgc
      301 agcacttctt cggatgagca gggagcttgc tattccgctt gttgcaacga atgatattca
      361 ttatacatat gcacaggatg ctgcgccgca tgatattctg ctgtgtcttc agacagggaa
      421 aaaactgcag gatgaaaacc gtatgcgtta tgagggcggg caatattatg tcaagtcaga
      481 ggaagagatg aaaaaactgt ttccttatgc acttggagcg cttgaaaata cacataaaat
      541 tgcggaacgc tgtcatgttg agatagaatt cggcaagtat aagctgccga aatttgatgt
      601 gccggagggc tatagtgcgt gggaatattt aaataaactc tgttttgagg gtttggataa
      661 gcgttatccg ggatatggaa aggatatgga agaacggctg cgctatgaat tggatattat
      721 tcacagtatg gggtttgtcg attattttct aattgtctgg gattttatcc gttttgcaaa
      781 atcaagcggg atcgcagtcg gaccgggacg tggttccgca gccggaagcg ttgtttctta
      841 ttcgcttgaa attacagata tcgatccgat gaaatataat ctgctgtttg agcggttcct
      901 caatccggaa cgtgtgtcaa tgccggatat cgatattgac ttctgctttg aaagaaggca
      961 ggaagtcatt gactatgtag tggagaaata cgggaaggat aaagtcgtac agatcgtgac
     1021 gtttggtacg cttgcggcaa agggtgtgat ccgtgatgtc ggaagagtta tggacctgtc
     1081 ctacgcctat gtggattcca ttgccaaaat ggttccgaat gaactgaata ttacactgga
     1141 gcgtgcactg gagcttaatc aggatctgaa aaaattatat gagaccgatg aacaggtgaa
     1201 gactctgatc gatatgtgca agcgtctgga aggactgccg agacatacgt ccatgcatgc
     1261 ggcaggcgtt gtgatctgtc aggaggcggc agatgagttc gtaccgctgt caagggcgtc
     1321 ggatggttcg atcacaacgc agttcgtgat gacgacgtta gaggagcttg gcttattgaa
     1381 aatggatttt cttggacttc gcacgctgac tgttatccag aatgcggtac atctggtaga
     1441 acaggatacc ggtgtgaagc
//
LOCUS       DAOSYE010000678         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79280_length_1457_cov_3.249643, whole genome shotgun sequence.
ACCESSION   DAOSYE010000678 DAOSYE010000000
VERSION     DAOSYE010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79280_length_1457_cov_3.249643"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1457
                     /locus_tag="PLU43_10485"
     CDS             <1..>1457
                     /locus_tag="PLU43_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO28880.1"
                     /translation="VLLHLYAEKGIEMLADINKNTVEFIPNFDETEKEPVVLPSRYPN
                     LLVNGTTGIAVGMATNIPPHNLQEVIAAVVKIIDNKINESRDTSIEELLPIVKGPDFP
                     TGGIILGTRGIDEAYRTGRGKIKVRGVTDIETMSNGKSRIVITELPYLVNKARLIEKI
                     ADLVRDKKIDGITDLRDESDRDGMRVVIELRRDANANIIVNQLYKHTQLQDTFGVIML
                     ALVNNEPKVMNLCEILRHYLAHQEDVVTRRTQYDLNKAEERAHILEGLLIALDNIDEV
                     IKIIRGSENVAKAKEQLMERFALTDVQAQAIVDMRLRALTGLEREKLENEYAELMKKI
                     AEYKAILGDEKLLLGVIRTEILVISDKYGDERKSQIGFDEFDITMEDLIPKDNTVIAM
                     TSLGYIKRMTVDNFKAQNRGGKGIKGMQTIEDDYIEDLLMTTTHNYVMFFTNYGRVYR
                     LKAYEVPESSRTSRGTAIINLLQLNPGEKISAIIP"
BASE COUNT          484 a          250 c          339 g          384 t
ORIGIN      
        1 gttctgttac atctttatgc cgaaaaaggc atcgaaatgc ttgccgatat caacaaaaat
       61 actgttgaat tcatacctaa ctttgatgaa acggaaaaag aaccggttgt tcttccaagc
      121 cgttatccaa atctgcttgt aaacggtacg acagggattg ctgttggtat ggctacaaat
      181 attccgcctc ataatctgca ggaagtaatt gctgctgttg ttaaaattat agacaacaaa
      241 atcaatgaaa gcagggatac ttcgattgaa gaactgcttc ccattgtaaa aggtcctgat
      301 tttcctacag gtgggatcat tcttggtaca agaggaatcg atgaagccta tcgtaccggc
      361 cgtggaaaaa taaaggttcg tggtgttacg gatatcgaaa caatgtcaaa cggaaaaagc
      421 cggatcgtaa ttacggagct tccttatctg gtcaataagg caagactgat cgaaaaaata
      481 gcggatcttg tcagagataa aaaaatagat ggaattacgg atcttcgtga cgaatccgac
      541 agagatggca tgcgtgtcgt aatagagctg cgccgtgatg ccaatgcaaa tattattgta
      601 aatcagctgt ataaacatac acagctgcag gatacgtttg gtgttattat gcttgcgctt
      661 gttaataacg aaccaaaggt catgaatctc tgtgaaattc ttcgccatta tcttgcccac
      721 caggaagatg ttgttacaag aagaacgcaa tatgatttaa ataaagcgga ggaacgggcc
      781 catatattag agggtttgct gatcgcgctc gataatatcg atgaagttat taaaattatc
      841 cgcggaagtg aaaatgttgc aaaagcaaaa gaacagctga tggaacggtt tgccctgact
      901 gatgtacagg cacaggcgat cgtagatatg aggcttcgtg ctttaacagg actggaaaga
      961 gaaaagcttg aaaacgaata tgcagagctt atgaaaaaaa ttgcggagta taaggcaatt
     1021 cttggtgatg aaaaactgct gcttggtgtt atcaggacgg aaattcttgt tatttccgat
     1081 aaatacggag atgagagaaa aagtcagatt ggttttgatg aatttgatat taccatggaa
     1141 gatctgattc caaaagacaa tacggtaata gcaatgacaa gtctcggata tatcaaacgt
     1201 atgacggtcg ataattttaa agcacagaac cgcggcggta aaggcattaa aggtatgcag
     1261 acaatagaag atgattatat tgaagatctg ctgatgacga ctacccataa ttacgtaatg
     1321 ttttttacaa attatggacg tgtataccgg ctgaaagcgt atgaggtgcc ggagtcaagc
     1381 aggacttcca gaggtacggc cattatcaat ctgcttcagt taaaccccgg tgaaaaaatc
     1441 agtgcgatca ttccgat
//
LOCUS       DAOSYE010000679         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79281_length_1457_cov_3.186163, whole genome shotgun sequence.
ACCESSION   DAOSYE010000679 DAOSYE010000000
VERSION     DAOSYE010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79281_length_1457_cov_3.186163"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1457
                     /locus_tag="PLU43_10490"
     CDS             <1..>1457
                     /locus_tag="PLU43_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO28881.1"
                     /translation="KKTIIIGGVAGGATTAARLRRRDESMQIIMLERGDYISYANCGL
                     PYYIGDVIKSRDALLLQKPEAMKNKFNIDVRVSSEVTKIIPEEKKIEINNRKTGEVYE
                     ETYDNLVLATGSSPLKPPIPGILADNIFTLWTIPDTDRIKAFITEKRPKRAAVIGGGF
                     IGLEMAENLHEQGIEVSVIEMQNQVMAPVDPEMANLIHEDMVRNQVDLILGDGVKEFH
                     QQDNGTRIELTSGREVEAELVILSIGVRPNSELAKQAGIALNKRGGVIVDDYLQTSIP
                     DIYAVGDVIEVTHFVTKDKTMVPLAGPANKQARILADNLAGDRKKYEGTLGAAVAKVF
                     DLDVASVGLNEKQLKAAGKVKDQDYFTALINQKSHAGYYPGATPLTLKMLFDKEGKIY
                     GGQIVGQEGVDKRIDTIASTMRLGGTVFDLEKLELSYAPPFSSAKDPVNMLGFVAENI
                     LHGLVSFIECDELDALQEGSAGEQDYTILDVTEDV"
BASE COUNT          429 a          269 c          410 g          349 t
ORIGIN      
        1 aaaaaagacg attattattg gcggagtggc cggaggagcg acaacggcgg caagacttcg
       61 gaggagagat gaatccatgc agatcattat gctggaaaga ggtgattaca tttcttatgc
      121 aaactgcgga ctgccttatt acattggaga tgtaataaaa agcagggatg ctcttttgct
      181 gcaaaagcca gaggcgatga agaataaatt taacattgac gtccgtgtat ccagtgaagt
      241 gacaaaaatt attccggaag aaaagaagat cgagatcaat aatcgaaaga ccggagaagt
      301 ttatgaagag acgtatgaca atcttgtact ggcaacaggt tcttcgccgc tcaagcctcc
      361 gattccgggt attttggcag ataatatttt tactctgtgg acaattccgg atacggaccg
      421 tatcaaagcc tttattactg aaaagcgtcc gaaacgtgcc gcagtgatcg gcggtggttt
      481 tatcggactt gaaatggcgg aaaatcttca tgaacagggg atcgaagtgt ctgttatcga
      541 aatgcagaat caggttatgg cgccggtcga tccggaaatg gctaacctga tccatgaaga
      601 tatggtaaga aatcaggttg atctgatcct gggcgatggc gtaaaggaat ttcatcagca
      661 ggacaatggt accagaatcg aattgacaag cggcagggaa gttgaagcgg aactggtcat
      721 cctttctatt ggcgtaaggc cgaacagtga gcttgcaaag caggcaggga tcgcattgaa
      781 caagcgggga ggcgttattg ttgacgatta tctgcagacc tcaataccgg atatttatgc
      841 ggtcggagac gtcatagagg tcacacattt tgttacaaag gataaaacca tggtgccgct
      901 tgccggaccg gctaataaac aggcgcgtat actggccgat aatctggcgg gagacagaaa
      961 aaaatatgaa gggaccctgg gagcggcagt tgcgaaagta tttgatctgg atgttgccag
     1021 tgtcggactg aatgaaaagc agctgaaagc tgcgggaaaa gtaaaggatc aggattattt
     1081 tacagcactg atcaatcaga aatcccatgc agggtattat ccgggcgcga cgcctcttac
     1141 cctgaagatg ctgtttgaca aagagggaaa aatttacggc ggtcagatcg tcggacagga
     1201 gggtgtggat aaaaggatcg acacgatcgc atcaactatg cgcttaggcg gtacggtgtt
     1261 tgatctggag aagctggagc tttcttatgc acctccgttt tcttccgcaa aggatcctgt
     1321 caatatgctt ggttttgtgg ctgaaaatat tctgcatggt cttgtaagct ttatcgaatg
     1381 tgacgaactg gatgctttgc aggaaggcag cgcaggagaa caggactata cgatactaga
     1441 tgtgacggag gatgtgg
//
LOCUS       DAOSYE010000680         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79576_length_1454_cov_2.850608, whole genome shotgun sequence.
ACCESSION   DAOSYE010000680 DAOSYE010000000
VERSION     DAOSYE010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79576_length_1454_cov_2.850608"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLU43_10495"
     CDS             complement(<1..434)
                     /locus_tag="PLU43_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28882.1"
                     /translation="MIEAAEISKSFDKIKAIDHVSAKIQDGAVFGLIGTNGAGKSTFL
                     RMLCGVLKPDKGEIFIEGIPVYENEEAKKQMFYISDEQYFFPNSTPAEMMKYYVSVYE
                     NFDRSRFLSLLSKFGLDKDRKINTFSKGMKKQVSVLLGICSGT"
     gene            complement(427..801)
                     /locus_tag="PLU43_10500"
     CDS             complement(427..801)
                     /locus_tag="PLU43_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO28883.1"
                     /translation="MFSIDYQDKRPIYEQVMENLQNLIVKGALEADSRLPSVRSFAME
                     LSINPNTIQRAYAELEKSGFIYTVKGRGNFVSDSKFWIQAEKKEIMQKVTESIQAAAR
                     LGVEAREIEKIIGECYGRKLND"
     gene            complement(943..1205)
                     /gene="ffs"
                     /locus_tag="PLU43_10505"
     ncRNA           complement(943..1205)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU43_10505"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1237..>1454)
                     /locus_tag="PLU43_10510"
     CDS             complement(1237..>1454)
                     /locus_tag="PLU43_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOO28884.1"
                     /translation="VQARIDKVVIGSMSPKSGCAGSVLNILQMEEFNHQVEIVSGVLE
                     EECSRMLSSFFAKLREKNKREKEEGSN"
BASE COUNT          375 a          377 c          231 g          471 t
ORIGIN      
        1 gttccgctgc aaattccaag aagtacggat acctgctttt tcattccttt agaaaacgta
       61 ttgatctttc ggtctttatc aagtccaaat ttactcaata acgataaaaa ccgcgatctg
      121 tcaaaatttt cataaacaga aacatagtac ttcatcattt ctgccggtgt cgaatttgga
      181 aaaaaatatt gctcatccga aatataaaac atctgttttt ttgcttcttc attctcataa
      241 acaggtattc cttctataaa tatttctccc ttatccggtt tcagcacccc gcagagcatt
      301 ctcaaaaacg tactttttcc agctccattc gtcccgatca gaccaaatac cgcgccatcc
      361 tgaattttag ccgatacatg atcgattgct ttgatcttat caaagctttt tgaaatttcc
      421 gctgcttcaa tcatttagtt tcctcccata gcactcccct attattttct ctatctcacg
      481 tgcttcaacg ccgagccgtg ccgcagcctg aatcgattct gtaacttttt gcatgatttc
      541 ttttttttcc gcctgtatcc agaatttgct gtcactgaca aaattaccgc gtcctttaac
      601 ggtataaata aaaccgcttt tttcaagctc cgcatatgct ctttggatcg tattcggatt
      661 aatggaaagt tccattgcaa aggatctgac agacggcagt ctgctgtctg cctccaaggc
      721 gccctttaca atcaaattct gcaaattttc catgacctgt tcataaatcg gtcgtttatc
      781 ctgataatca atagagaaca tgggcaccct ccttaactaa gtgtattaat attcttaata
      841 cacttaatat acacagtttt cttttatctg tcaaaccttt tctgcaaaag cgtccttgat
      901 agacgccaaa aaagccggat atctccgact tttatacaaa atccgtgcgt ccggctttgt
      961 gcgcctcttc gcatgcgtta cctctgcagt taactcaatc caggcaccct tacggcacac
     1021 agaaggttct atttagtgct gcttcattcc tgacctgaca cgattcatag atttctgttg
     1081 cgtaagaccc agatatcaac gccacttaca aagggctgcc atgcaaatca gcacccgggg
     1141 ccggacatca ctcctgctat agcggatttc aggtacaggg aaccgctgct tccccagctg
     1201 cacggatttt tattatactg tgtttccttg tttttgtcaa ttgcttccct cttctttttc
     1261 tcttttattt ttttcacgca gttttgcaaa aaaagaagac agcatccggc tgcattcctc
     1321 ttccagcaca ccggatacga tttctacctg atgattaaat tcctccattt gcaaaatatt
     1381 caaaaccgag cctgcacagc cggatttcgg actcatactg ccgatcacaa ctttatcgat
     1441 cctggcctgt acgc
//
LOCUS       DAOSYE010000681         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79589_length_1454_cov_2.578985, whole genome shotgun sequence.
ACCESSION   DAOSYE010000681 DAOSYE010000000
VERSION     DAOSYE010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79589_length_1454_cov_2.578985"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLU43_10515"
     CDS             <1..349
                     /locus_tag="PLU43_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO28885.1"
                     /translation="RPMDNHLSGGESKRIEIATVLARKASVLVFDEPEAGIDLWSFTS
                     LIDTFKTLKEEQDGALLVISHQERILDIADTIVVIADGKTRISGNKETVLPKLLIDEH
                     SRQCPLGKEVSNE"
     gene            342..1268
                     /locus_tag="PLU43_10520"
     CDS             342..1268
                     /locus_tag="PLU43_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOO28886.1"
                     /translation="MNNITEQLLQTITDWDGIFNGAYSLREDGQCIDAHSSENIRIVP
                     KTDKPGIDIYVSSKAVRETVYIPACVTHGSVKDLVYNDFHIQSGADITIIAGCGVHTD
                     SDEGVAHSGVHRFLLEPFSHVVYKEKHLGTGKGSGKKIIDPITEAELSEGSVLEMETL
                     QLGGVDTTIRRTTAALAKKAKLIIHESILTDFENKAETDFLVSMNGEDSSVDLISRSV
                     AKGSSTQKYHSHIIGSCRCSGHSECDAILVGNGKVNASPELDAENLDASLIHEAAIGK
                     IAGEQIVKLMTLGLTEEEAESKIIEGFLTSAE"
BASE COUNT          428 a          324 c          356 g          346 t
ORIGIN      
        1 ccgcccaatg gacaatcatt tatccggcgg agaaagcaaa cggatcgaga tcgcaacagt
       61 gcttgccaga aaagcgagcg tactcgtatt tgacgaaccg gaggcaggga tcgatttatg
      121 gagttttaca agcctgatcg atacgttcaa gacattaaag gaagaacagg acggcgcact
      181 gcttgtcatc tcccaccagg aacgtatcct ggatatcgca gatacgatcg ttgttatagc
      241 ggacggcaaa accagaattt ccggcaataa agaaaccgtg cttccaaagc tgctgatcga
      301 cgaacacagc aggcaatgcc cgctcggtaa ggaggtttcc aatgaataat attacggaac
      361 aattattaca gacgatcact gactgggatg gcatttttaa cggtgcatac agtctgcgtg
      421 aggacggaca gtgtattgat gcacactcct ccgaaaatat ccggatcgtt ccaaaaacag
      481 ataaaccggg aatcgatatt tatgtctcat caaaagcagt acgggagacc gtgtatattc
      541 cggcctgcgt aacgcacggc tcagtaaagg atcttgtata taacgatttt catattcagt
      601 ccggcgccga cataacgatc attgccggct gcggtgtcca cacggattcc gacgaaggcg
      661 ttgcccacag cggtgttcac cggttccttc tggaaccgtt ttcccatgtc gtatacaaag
      721 aaaagcacct tggtaccggc aaagggtccg gaaaaaagat aatcgacccc attacggagg
      781 ctgaactttc agaaggctct gttttggaaa tggaaaccct gcagctcggc ggtgttgata
      841 caacgatccg caggacgact gcagcgcttg caaaaaaagc aaaactcatc atccatgaaa
      901 gtattttgac ggattttgaa aataaagccg aaactgattt tctcgtgtca atgaacggag
      961 aggattccag cgtggacctg atctcgcgtt ctgttgccaa aggcagttcc acacagaaat
     1021 atcattctca tattatcggc agctgccgct gcagcggtca ctccgaatgt gatgcgatcc
     1081 ttgtcggcaa cgggaaggtc aatgcatcac cggaactgga tgcggaaaat ctggatgctt
     1141 ccctgatcca cgaggccgcc attggcaaga tcgccggtga acagatcgta aaactgatga
     1201 cacttgggct taccgaggaa gaagcggaaa gcaaaatcat agagggattt ctgacttccg
     1261 cggaatagtt ttacttgctt tttcttgatt ttcccgtata attagaaccg gtggtttaaa
     1321 acaaacaggt tcataaatac agtaaatagg agaattttca tgatcagtgc taacaatgtg
     1381 acgcttcgtt tgggaaaaaa agcgttattt gaagatgtta atattaaatt tacggaaggg
     1441 aactgctatg gtct
//
LOCUS       DAOSYE010000682         1452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_79752_length_1452_cov_2.495347, whole genome shotgun sequence.
ACCESSION   DAOSYE010000682 DAOSYE010000000
VERSION     DAOSYE010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79752_length_1452_cov_2.495347"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLU43_10525"
     CDS             <1..909
                     /locus_tag="PLU43_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO28887.1"
                     /translation="ALVDEVGKGETLSTAMRKQDRIFPPMFVNLVEAGEATGSLETAF
                     ERMAVQFEKEAQLKAIVKKAMIYPIIVACVAIGVIFAMMTFVIPNFMSMFADMDIEMP
                     AFTQFIIDLSDFFVAYWWAIIAVVLLVIVLLRIYARTTNGRVVFSTIKIKLPVFGRLQ
                     IKTASARLARTLSTLLRAGVPMIQALEITANSMSNNILFQRAIYNAKDQVSNGVALSK
                     PLKMCGLFPPMVVHMVSIGEETGNLEDMLENIAAYYEEEVTEATEQLTAVMEPMIILI
                     MALVVGILIMAIMQPMLTLYDAVGNM"
     gene            887..1024
                     /locus_tag="PLU43_10530"
     CDS             887..1024
                     /locus_tag="PLU43_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28888.1"
                     /translation="MTQLEICKWYEESGLYEQTGPDLYIEDVNDAVQRGRDSVGQKGT
                     E"
     gene            1054..>1452
                     /locus_tag="PLU43_10535"
     CDS             1054..>1452
                     /locus_tag="PLU43_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO28889.1"
                     /translation="MNKKQKSLNNKGFSLVELIVVIAIMAILVGALAPQLTKYIEKAK
                     RSSDVQAAGTIYTAVQMALADPDIEQPTLPHAASAVTAITSATGTDEFQPAIAETVGG
                     TANLSPTYKSAAFTGTTAKVAIDSNGNVTLT"
BASE COUNT          398 a          289 c          398 g          367 t
ORIGIN      
        1 gctttagtgg acgaggtcgg aaagggcgag acgctgtcaa cggccatgag aaaacaggat
       61 cggatatttc cgccgatgtt tgtcaatctt gtagaagcag gagaagcgac cggcagtctg
      121 gagacagcat ttgagcgtat ggccgttcag tttgaaaagg aggcacagct caaggcaatc
      181 gttaagaagg caatgatcta tccgatcatt gtagcctgcg ttgcgatcgg cgtgattttt
      241 gcgatgatga cgttcgtcat tcccaatttc atgtcgatgt ttgcggatat ggatattgag
      301 atgccggcat ttacgcagtt tatcattgat ctgagcgatt ttttcgtagc ctactggtgg
      361 gccatcattg cagttgttct gctggtcatt gtattgctta ggatatatgc gcgtacaaca
      421 aacggaagag tcgtattttc cacgatcaag atcaagctgc cggtatttgg gagacttcag
      481 atcaagacag ccagtgccag gctggcaagg acactgtcca ctctgcttag agccggtgtt
      541 ccgatgatac aggcgctgga gatcacagct aattcgatga gcaataatat tttatttcag
      601 cgtgcaattt ataatgccaa ggatcaggtc tcaaacggtg ttgccctgtc caagcctttg
      661 aaaatgtgcg gactgtttcc gccgatggtc gttcatatgg tatcgatcgg cgaggagacc
      721 ggtaatctgg aagatatgct cgaaaatatt gcggcttatt acgaggagga agttacagag
      781 gcgactgaac agctgacagc agttatggag ccgatgatca ttctgatcat ggcacttgtc
      841 gtaggtattt tgatcatggc gatcatgcag ccgatgctga cactttatga cgcagttgga
      901 aatatgtaaa tggtatgaag aatccggact gtatgagcag acgggaccgg atctttatat
      961 agaggatgtc aatgatgcag tgcagagagg gagagacagc gtggggcaga agggcaccga
     1021 atagcataga ttatgaaaag gagataaagg actatgaaca aaaaacaaaa atcattgaat
     1081 aacaaaggct tctcgctcgt ggaactgatc gttgttattg ccattatggc aattctggta
     1141 ggcgctctgg ctccgcagct gacgaagtat atcgaaaagg ccaaaagaag ttcggatgtg
     1201 caggcagccg gtacgatcta taccgcggtt caaatggctt tagctgatcc tgatatcgaa
     1261 cagccgacat tgccgcatgc agccagtgcg gttacggcaa ttaccagtgc gacgggtact
     1321 gatgaatttc agcctgcaat tgcggaaact gtaggaggta ctgctaattt atcaccgaca
     1381 tacaaatcag cagcttttac cggcacaacg gctaaggttg cgatcgactc aaatggaaat
     1441 gtgacattga cg
//
LOCUS       DAOSYE010000683         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80042_length_1449_cov_2.472740, whole genome shotgun sequence.
ACCESSION   DAOSYE010000683 DAOSYE010000000
VERSION     DAOSYE010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80042_length_1449_cov_2.472740"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /gene="ybeY"
                     /locus_tag="PLU43_10540"
     CDS             complement(<1..275)
                     /gene="ybeY"
                     /locus_tag="PLU43_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOO28890.1"
                     /translation="MTCHIDMEVENKLDFPYEELFEKIAATVLKEEKCPYEAVVSLLI
                     TDNDSIREINREYRHTDKETDVLSFPMAEYKIPADFSMLDGQADCFDP"
     gene            complement(284..1300)
                     /locus_tag="PLU43_10545"
     CDS             complement(284..1300)
                     /locus_tag="PLU43_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO28891.1"
                     /translation="MTESITEQSITIEPEHAKNIYGTFDVFLKKIEKSFQVTIIDRND
                     EIKIIGAKQNVTGALTILKQLQEMSKRGNIIHEQNVDYAIAMAMEEKQNIVLEIDKDL
                     ICHTTNGKPIKPKTLGQKEYVDAIRKKMIVFGLGPAGTGKTYLAMAMAITAFKNNEVG
                     RIILTRPAIEAGEKLGFLPGDLQSKVDPYLRPLYDALYQIIGADSFQKNMEKGLIEVA
                     PLAYMRGRTLDNAYIILDEAQNTTPAQMKMFLTRIGFGSKVIITGDRTQKDLAPSAVS
                     GLDMALRVLGKIEEVGFCNLTSKDVVRHPLVQKIVKAYEDYEEKQNSNLGNSYRKNSK
                     KRGR"
BASE COUNT          362 a          334 c          265 g          488 t
ORIGIN      
        1 ggatcaaaac agtcagcctg tccgtcaagc atggaaaaat ctgccgggat cttatattcg
       61 gccatcggga aggaaaggac atctgtttct ttatctgtat gtctgtattc cctgttgatt
      121 tcacgaatag agtcattatc cgtaatcagc aggctgacga cagcctcata cggacatttt
      181 tcttctttta aaacagtcgc tgcgattttt tcaaacaatt cctcataggg aaaatccagt
      241 ttgttctcta cttccatatc aatatgacag gtcatgatcc ggtttatctt cctctctttt
      301 tgctgttttt tctataactg ttacccaaat tggaattctg tttttcttca taatcttcat
      361 aagctttgac aattttctgg acaagcggat gtctgacaac atctttgctt gtcaggttac
      421 aaaatccgac ctcttcaatt tttccaagca cgcggagcgc catgtcgagt cctgatactg
      481 cggacggagc cagatctttc tgtgtccggt ctccggtaat aatgactttg gaaccaaatc
      541 cgatacgcgt caggaacatt ttcatctgtg caggagtcgt attctgggct tcatccaaaa
      601 taatatatgc attatcaagt gtacgccccc gcatatatgc aaggggtgct acttcgatca
      661 gacctttttc catattcttc tgaaaactgt ccgcaccgat gatctgataa agtgcatcat
      721 aaagcggacg caggtaggga tctactttac tttgcagatc acccggcaga aaaccaagtt
      781 tttcacctgc ttcgatcgcc ggacgtgtca aaatgatcct gccgacttca ttatttttaa
      841 aagcagtgat cgccatagcc atggccagat aagtctttcc ggttccggca ggtcccagtc
      901 caaatacgat catttttttc cgaatggcat caacatattc tttctgaccg agcgttttgg
      961 gtttgatcgg ttttccattc gtcgtatgac agatcagatc cttgtcgatt tcaagcacaa
     1021 tgttctgctt ttcttccata gccattgcaa ttgcataatc aacattctgt tcatgaatga
     1081 tatttcccct tttagacatt tcctgcagct gctttaatat agtcagagcg ccggtcacat
     1141 tttgttttgc cccgataatt ttgatttcat catttctgtc aatgatcgtt acctgaaatg
     1201 atttttctat ttttttcaga aaaacatcaa atgttccata aatatttttg gcatgctctg
     1261 gttcgatcgt aatgctctgt tcggtaatac tttccgtcat ggaattccca gctttccttt
     1321 atataattat ctgatgattt ttttgataaa aacaggcggg acaacaggac tctttgtaat
     1381 tgttattgca ccggccagac gttcccttgc ctctgcaagc ttattttcat catccgcata
     1441 caaaacagc
//
LOCUS       DAOSYE010000684         1448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80134_length_1448_cov_2.597990, whole genome shotgun sequence.
ACCESSION   DAOSYE010000684 DAOSYE010000000
VERSION     DAOSYE010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80134_length_1448_cov_2.597990"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="PLU43_10550"
     CDS             complement(<1..1367)
                     /locus_tag="PLU43_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOO28892.1"
                     /translation="MPTAISEAAVQDIVKEVIAKMQISGTMSNRHGVFTDMNEAIAAA
                     KKAEAVVRNLSMDQREKIISNIRKKTHENAEILARMGVDETGMGNVGHKILKHHLVAD
                     KVPGTEDIKTEALSGDRGLTLVEMGPFGVIGAITPSTNPSETVLCNAMGMIAGGNTVV
                     FNPHPAAIKTSIYAINLVNEASIEAGGPDNVACTVEKPTLETSNIMMKHKDIPLITAT
                     GGPGVVTAVLSSGKRGIGAGAGNPPALVDETADLKKAAEDIVNGCTFDNNLPCIAEKE
                     IVAVDSVVDELMHYMVTEQGCYLASAQEQDKLIKTVFTEKGTLNRKCVGRSAKALLAM
                     IGVTVPDNIRCITFEGPKEHKLIAEELMMPILGVVRAKDFDDAVEQSVWLEHGNRHSA
                     HIHSKNVDRITAYARAIDTAILVKNGPSYASLGFGGESICTFTIASRTGEGLTSAKTF
                     TKRRR"
BASE COUNT          312 a          402 c          292 g          442 t
ORIGIN      
        1 catcttcttc tctttgtgaa ggtctttgca cttgtcaggc cctctcctgt acggcttgcg
       61 atcgtaaatg tacagatact ctcaccgcca aaaccaagag acgcgtaaga aggtccgttc
      121 ttaacaagaa ttgccgtatc gatcgctctc gcataagccg ttatacggtc aacgttttta
      181 gaatgaatat gtgccgaatg gcgattgcca tgctcgagcc agaccgactg ttctacagca
      241 tcatcaaagt cctttgccct gacgacaccg agtatcggca tcatgagctc ttctgcgatc
      301 agcttatgct cctttggtcc ttcgaatgta atgcagcgga tattatcagg tacggtcaca
      361 ccgatcatgg caagcagtgc ttttgcactt ctgccgacac attttctgtt caaagttccc
      421 ttttccgtaa acaccgtttt gatcagcttg tcctgttcct gtgcagaagc cagataacag
      481 ccctgctccg taaccatata gtgcatcaat tcgtcaacga ccgaatcgac cgcaacgatc
      541 tccttttccg caatacatgg aagattattg tcaaacgtac atccattgac aatatcctct
      601 gccgcttttt tcagatccgc cgtttcatct acaagtgccg gcgggtttcc tgctcctgca
      661 ccgatcccgc gtttaccgga agaaagtacc gcggttacga cacccgggcc gcctgttgcg
      721 gtgattaacg gaatatcttt atgtttcatc ataatattgc tcgtttccaa tgtcggtttt
      781 tctaccgtac aggcaacatt atccggtcct cctgcttcaa tggacgcttc gtttacaaga
      841 ttgatcgcat agatcgatgt tttgattgct gcaggatgcg gattaaatac gaccgtattg
      901 cctcctgcga tcattcccat ggcattgcac agtaccgttt cactcggatt ggtagacgga
      961 gtgattgctc cgatcacacc gaaaggaccc atttccacaa gcgtcagacc tctgtctcct
     1021 gacagcgctt ctgttttgat atcctctgtt ccgggaactt tatcggctac cagatgatgc
     1081 tttaatattt tatgaccgac gtttcccatt cccgtttcat cgactcccat tctcgcaagg
     1141 atctctgcat tctcatgggt ttttttcctg atgtttgaaa tgatcttctc tctctgatcc
     1201 attgagagat ttctgacgac tgcctctgct ttctttgctg ccgcaatcgc ctcgttcatg
     1261 tccgtaaaga cgccgtgccg gttactcatc gttccggaaa tctgcatctt agcaatgact
     1321 tctttcacaa tgtcctgcac agccgcctca cttattgctg taggcatgtg attacctcct
     1381 ctaatatgct cacgccatat tctgcaagct ttgtatcctg ttctgccgtt ccgccgctga
     1441 caccgagt
//
LOCUS       DAOSYE010000685         1446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80292_length_1446_cov_3.585191, whole genome shotgun sequence.
ACCESSION   DAOSYE010000685 DAOSYE010000000
VERSION     DAOSYE010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80292_length_1446_cov_3.585191"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1292)
                     /locus_tag="PLU43_10555"
     CDS             complement(84..1292)
                     /locus_tag="PLU43_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28893.1"
                     /translation="MTKLKKLVAIGLSLVMVTGSLAGCGAVTTTTPASDAAATDTAAS
                     DTAAAADTSGDSLEGKHVFMFKSTGNSFGDLMFEGFSEYMESQGQTAVYKSPAETTVA
                     AQVQLLDELITQKVASITISTNGDAGYDEVFKKAQDAGIPITSIDSAANPEYRVTHVN
                     QAEVADIGAYLVQAAVLITLGVDYPEDGDMKAAVEENLADYSGDEIVLGVLSASIDTP
                     VQNSWIAAMEEELKDSCYSGKVSATLDKKYGNDDLTESTTQAQAFIAENKVDCIISPT
                     TVGIAAAGQCLKTSSSDIRLTGLGLPSEMQSFMPSAESDDAFSYPCPYMMLWDVIHLG
                     AVSAASTLATIDGKFDGAIGSSFDMAQFRDYAPETYTAYDNGDGGTAVLAGVPYVFYK
                     GNMAEWIDKL"
BASE COUNT          344 a          381 c          298 g          423 t
ORIGIN      
        1 actatattta aaaagcaata cagagtccgc catacggacg gactctcctt gcctgcataa
       61 atgcaattga ccgcggatat ggattacagc ttgtcgatcc actctgccat gttgccttta
      121 tagaatacgt atggaacacc tgcaagcact gcagtgcctc cgtctccgtt atcataggcc
      181 gtataagttt ccggtgcata atcacggaac tgtgccatgt caaaggaaga accgatcgct
      241 ccgtcaaact tgccatcgat cgttgcaagt gtggacgctg cggatacggc acccagatga
      301 attacatccc aaagcatcat gtaaggacat ggataagaaa atgcatcatc ggattccgcg
      361 cttggcataa aggactgcat ctcagaaggt aatccaagac ctgtcagtct aatatcggag
      421 ctggatgtct ttaggcactg tccggctgca gcaataccaa ctgttgtcgg agaaataata
      481 caatccactt tgttttcagc aataaatgcc tgtgcctgtg ttgttgattc cgtcagatcg
      541 tcgtttccgt acttcttatc aagtgtagca ctgacttttc cggaatagca gctgtctttc
      601 aattcttctt ccatcgctgc gatccagctg ttctgtaccg gtgtgtcgat agaagccgaa
      661 agaacgccga gtacgatctc atcaccagaa taatctgcaa gattttcttc cacagctgcc
      721 ttcatatcgc cgtcttccgg ataatcaaca ccaagtgtga tcaaaactgc agcctgcaca
      781 agataagcgc cgatatccgc aacttctgcc tgattgacat gcgtcacacg gtattccgga
      841 tttgctgccg agtcaatgga tgtgatcgga attcctgcgt cctgtgcttt tttgaatact
      901 tcgtcataac ctgcatcgcc gtttgtggaa attgtgatcg atgcaacctt ctgcgtgatc
      961 agctcgtcca gcaactgaac ctgtgcggct accgttgtct ccgcagggct cttatatact
     1021 gccgtctgtc cctgtgattc catatactca ctgaatcctt caaacatcag atctccaaag
     1081 gaattacctg ttgatttaaa cataaataca tgtttgcctt ccagggaatc tcccgaagtg
     1141 tctgccgctg cagcagtgtc gcttgcagcc gtatcggttg ctgccgcatc acttgccggt
     1201 gtcgttgtcg taaccgcccc gcagcctgcc agtgaacccg taaccattac cagactaagt
     1261 ccgatcgcca ctagtttttt taattttgtc atacctttca tacctccata atgaaatatt
     1321 tttagtttct atataatcag cgcctttgca ccgtttatat cattttgtca aaccgctcct
     1381 ttgcttttct ggcatctgcc ttcaggtccc gtttcagact gtctgtctgt tctttgcatt
     1441 ttgtcc
//
LOCUS       DAOSYE010000686         1446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80307_length_1446_cov_2.949676, whole genome shotgun sequence.
ACCESSION   DAOSYE010000686 DAOSYE010000000
VERSION     DAOSYE010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80307_length_1446_cov_2.949676"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLU43_10560"
     CDS             <1..808
                     /locus_tag="PLU43_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28894.1"
                     /translation="VALTANAIKGIQEMFLENGFDDFLSKPIDIKRLGQIMYKWLPKE
                     KQIDKDSYEEKVGTGTLDELLCRARAVFSKVSNFSVDSSLALCDNNIEIVLEVINIYV
                     KSAKNVFLKIEKAYEQKDFHNYGIEVHGVKSASKNIGADLLSEEARMLEVESKAKNEV
                     YVMTHHEQFLADYHRLVEELSESLKLIFPKETRKLKPISEEKYQSLLMNAKEALENWN
                     TKDGMDMINELLSCELEEEARNSLEDILENIELFDFDIAIKKIRKLEGES"
     gene            819..>1446
                     /locus_tag="PLU43_10565"
     CDS             819..>1446
                     /locus_tag="PLU43_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOO28895.1"
                     /translation="MIHIFIINPVAGLRDRSENIRQFLERKHDFSYLVFDTEKEGNET
                     DIVKRMLRLFEDEKIRFYVCGGSGSFANAISGIEEKEFARVEMAHYPCGMTNDFIKNF
                     GRSQNLFCNMDNLIRGEVLQVDYVKNIVPEGADPYHHVLFSTIGLTERVERFANRIKI
                     VGSMNSGFLYLLSFMLTFLFNPTVEYTLEIDGEDCSGEYDMIYIGNSIC"
BASE COUNT          455 a          234 c          363 g          394 t
ORIGIN      
        1 cgttgccctg acagcgaatg cgatcaaagg tattcaggaa atgtttctgg aaaacggatt
       61 tgatgacttt ttatccaaac cgatcgatat caaacgtctc gggcagatca tgtataaatg
      121 gctgccgaaa gagaagcaga tcgacaagga ctcctatgag gaaaaggtgg gtacaggcac
      181 tttagatgag ctgctttgca gagcaagggc tgtgttttca aaagtttcca atttttccgt
      241 tgacagcagt cttgcccttt gcgacaataa tattgaaatc gtgctggaag tcattaatat
      301 ttatgtgaaa tccgcaaaaa atgttttcct gaaaattgaa aaggcttatg agcagaagga
      361 tttccataat tacgggattg aagtgcatgg agtcaaaagt gcttcaaaga atatcggtgc
      421 ggatctgctt tcagaagaag cccggatgct ggaagtggaa agcaaggcaa aaaatgaagt
      481 gtatgtcatg acgcatcatg aacagtttct ggcggattac cacaggcttg tggaagagct
      541 ttcggaaagc ttaaagctga ttttcccgaa ggaaaccagg aaattgaaac caatttccga
      601 agaaaaatat caaagtcttc ttatgaacgc aaaagaagca ctggagaact ggaatacaaa
      661 agatggcatg gatatgatca acgaactgct ttcctgcgag ctggaagaag aggcgagaaa
      721 cagcctggaa gatattcttg aaaatattga gctgtttgat tttgatatcg cgatcaaaaa
      781 aataagaaaa ctggaaggtg aaagttaggt gacgtggaat gatacatatt tttataatta
      841 atccggtcgc aggacttagg gaccgttcgg aaaatattcg gcagtttctt gaaagaaagc
      901 acgatttttc ttatcttgta tttgatacgg aaaaagaagg aaatgaaact gatattgtga
      961 agagaatgct caggctgttt gaagatgaga agatcaggtt ttatgtgtgc ggcggttcag
     1021 ggtcgtttgc gaatgcgatc agcgggatcg aagaaaaaga atttgcccgg gtggagatgg
     1081 cgcattatcc gtgtggaatg acaaatgatt ttattaaaaa ttttggccgc agccagaatc
     1141 ttttctgcaa tatggataat ctgatccgcg gagaagtgct gcaggtcgat tatgtgaaaa
     1201 atattgtgcc cgaaggagca gatccgtatc atcatgttct tttttccacg atcggtctga
     1261 cagaaagagt agagcgtttt gccaacagga tcaaaattgt cggcagtatg aattccgggt
     1321 ttttatatct gctctcattt atgctgacct ttctttttaa tccgacagtt gagtatacgc
     1381 ttgagatcga cggtgaagat tgcagcggcg agtatgatat gatctatatc ggaaacagta
     1441 tctgta
//
LOCUS       DAOSYE010000687         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80431_length_1445_cov_2.222302, whole genome shotgun sequence.
ACCESSION   DAOSYE010000687 DAOSYE010000000
VERSION     DAOSYE010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80431_length_1445_cov_2.222302"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLU43_10570"
     CDS             <1..1220
                     /locus_tag="PLU43_10570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO28896.1"
                     /translation="YFPDTPVYLRAVDGHSEWMNTAALKESGYMSGWEPAFGSVDRSE
                     DGELTGLVREGGDALSRKYDTKLPPEEEEFLQKRLIKRLNAVGITSFTEMGATVPEGI
                     TELYRHVKKIDDAGEMTIRLFLYPGTDIDPSRIGEIDAYKEIYRSDTLKIAGLKGFAD
                     GVTSTFTAAMTEPYTGREDKGYLNYPEEKYREWVLQANRHGYGTRVHCIGDYAVHALL
                     DAYEASNLANQNHGLRNTIEHIEMIRPEDIKRFAELGVVPSMQPLHLPLDEFDKINHC
                     GVERSHYEWVHKSILDTGAVLALGTDFPVADYTPLYNVYQAVTRKGLNGVQYGPYTLD
                     ECLTVAQTLRAYTWGAAYAVGMEQKTGTLEAGKYADITVIDKNLLTIPEEDIVNAKIL
                     LTVMDGRVVYELP"
     gene            1347..>1445
                     /locus_tag="PLU43_10575"
     CDS             1347..>1445
                     /locus_tag="PLU43_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO28897.1"
                     /translation="MKELEVIIKPEKLETVKEILDEFHCGGITISSV"
BASE COUNT          442 a          275 c          399 g          329 t
ORIGIN      
        1 ggtattttcc ggacacaccg gtatatttaa gggctgtgga cggacattcg gagtggatga
       61 atacggcagc actgaaggaa agcggctata tgagcggatg ggaacctgct ttcggcagtg
      121 tggacagatc tgaggacggg gaactgaccg gccttgtcag agaaggggga gacgcactca
      181 gcaggaagta tgatacgaag ctgccgcctg aagaagagga atttctgcaa aaaaggctga
      241 tcaaaagatt gaatgccgtt ggaatcactt cttttacgga aatgggtgca accgtaccgg
      301 agggaattac ggagctttat cgtcatgtga agaaaataga tgatgccgga gaaatgacga
      361 tccgtctgtt tctgtatccc ggaactgata ttgatccgtc cagaatcgga gaaatagacg
      421 catataaaga gatctatcgg tcggatacat taaaaattgc aggtctgaag ggctttgcgg
      481 acggggtgac atcgaccttt acggcagcta tgacagaacc gtataccggc agggaagaca
      541 aaggctattt aaactatccg gaagaaaaat acagggaatg ggtgctgcag gcaaaccggc
      601 acggatacgg aacaagggtg cactgcatcg gcgactatgc cgtacatgcg cttctggatg
      661 cctacgaagc ttccaacctt gcaaatcaaa accacggtct gcgcaatacg atcgagcata
      721 ttgaaatgat cagaccggaa gatatcaaga gatttgcaga actgggagtc gttccgtcca
      781 tgcagccgct gcatcttccg ctggatgaat tcgacaagat caatcattgc ggggtggagc
      841 gcagccatta tgaatgggtc cataagtcga ttttggatac gggtgcagtg cttgcactcg
      901 gaacagattt tccggttgcg gattataccc ctttgtataa tgtatatcag gcagttaccc
      961 gcaaaggcct gaacggtgta caatacgggc cgtatacact ggatgaatgc ctgacggtag
     1021 cacagacgct gcgcgcttat acatggggcg ctgcctatgc ggtaggaatg gagcagaaaa
     1081 cagggacact tgaagccgga aaatatgcgg acatcacggt gattgacaaa aatctgctga
     1141 caataccgga agaagatatt gtgaatgcaa aaatactgct gacagttatg gatggcagag
     1201 ttgtatacga attaccataa aagaagtaga tataattatt tgtaattata aaaattatat
     1261 aagaggtgta tagacacact tcctttcgga attttataat aatgtcagca ggataaaact
     1321 gctttggcgg aaagagaggg atggcaatga aggaattgga agtaatcatt aaacccgaga
     1381 agcttgaaac cgttaaggag attttggatg agtttcattg cggaggcatc acgatatcca
     1441 gcgta
//
LOCUS       DAOSYE010000688         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_80796_length_1441_cov_3.103175, whole genome shotgun sequence.
ACCESSION   DAOSYE010000688 DAOSYE010000000
VERSION     DAOSYE010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80796_length_1441_cov_3.103175"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..897)
                     /locus_tag="PLU43_10580"
     CDS             complement(139..897)
                     /locus_tag="PLU43_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28898.1"
                     /translation="MRISEKRVKYIPQFLMIIGASNLCLTLLVAGMRRLSETDTMLFG
                     IAAYDLLSLIEPYGKMLWETGVVCFFFILIVRGKWRLDKSLILLWSFIIIEIQCIYYI
                     SSNYYGTMIDSMLNFSGTAAYVSFYAGTHVFKYVLMYMSIVFAIFTTGLFLEDRLLPV
                     IAVVMGIIYATCFGIQSMPTITLPDGSSVGVVIPAAIFHIAQTLIIFLTGLYLRLRYH
                     LKEKEVLTAVKPEAIPVKRKTRKKQKKQRKHKRR"
     gene            complement(909..>1441)
                     /locus_tag="PLU43_10585"
     CDS             complement(909..>1441)
                     /locus_tag="PLU43_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOO28899.1"
                     /translation="IIENIRSAKKESVQKKTDELKIRPDKKEITINILKFVIGTVGIV
                     WGADLLVDNGSELARIAGISESIIGVTLVAVGTSLPELVTTITAILKKQSSLSIGNII
                     GANIIDLTIILPLCAIVSGKALPVSTQMTSLDMPVCIAVGLIAMVPALISQKFHRWQG
                     VLLLACYAVYMGVLFI"
BASE COUNT          444 a          352 c          247 g          398 t
ORIGIN      
        1 tgatataaaa aattactgat cgcatcagat gttccgggtc tgaaatagca tactgcgatc
       61 aaaacaaaaa gcgccgtacc taaagctccc aatattccca aaaggatctt tatgattttc
      121 atagcaaccc tcccacttct atcgacgttt atgtttcctt tgcttcttct gcttttttct
      181 ggtttttctt tttaccggta tggcttccgg ttttactgca gttaaaacct ctttctcctt
      241 tagatgatac cgtaatctca aatacaatcc tgttaaaaaa ataatcagcg tctgtgcaat
      301 atgaaaaatc gcggcaggta tgacgacgcc gacagagctt ccgtccggaa gcgtaatcgt
      361 cggcatactc tgtataccaa agcacgttgc ataaatgatc cccataacga cagctatcac
      421 aggcagcagt ctgtcctcca gaaaaagacc cgttgtaaaa atcgcaaata cgatcgacat
      481 atacatcaat acatatttaa aaacatgcgt tcccgcataa aaagaaacat atgccgcagt
      541 tcccgaaaaa ttcagcatcg aatcgatcat cgttccgtaa tagttactgg aaatataata
      601 aatacactgt atttctataa taataaaaga ccagagtaaa ataagtgact tatccaggcg
      661 ccatttccct ctgacgatca atataaaaaa gaaacataca acgccggttt cccacaacat
      721 tttcccgtat ggttctatca gtgacagcag atcataagca gctattccaa acagcatcgt
      781 atcggtttca gacagtcttc tcatacctgc tacgagcagt gtcaggcata aattggaagc
      841 tcctatgatc atcaaaaatt gggggatata ttttactctc ttctccgata tcctcatgca
      901 ccctccgttc aaataaacag cacgcccata taaaccgcat agcaggcaag cagcaggaca
      961 ccctgccatc tgtggaactt ctgtgatatc agggccggaa ccatagcgat cagaccgact
     1021 gcgatacaga ccggcatatc cagactggtc atctgtgtgg aaaccggcag ggcttttccg
     1081 gaaacaattg cacataacgg taaaataatc gtcagatcaa taatattggc tccaataata
     1141 ttcccgatcg acagggaaga ctgcttttta agaatggcag tgatcgtcgt aacaagctcc
     1201 ggaagagaag ttccgaccgc caccaatgtt actccgataa tactttccga tataccggcg
     1261 attctggcaa gctcgcttcc gttatccaca agcagatccg ctccccatac aattccaaca
     1321 gtacctatga caaatttcaa aatattgatc gtaatttctt ttttatcagg cctgatcttt
     1381 aattcgtccg tcttcttctg cacagattct ttttttgcac ttctgatatt ttcaataata
     1441 a
//
LOCUS       DAOSYE010000689         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81002_length_1439_cov_2.947977, whole genome shotgun sequence.
ACCESSION   DAOSYE010000689 DAOSYE010000000
VERSION     DAOSYE010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81002_length_1439_cov_2.947977"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLU43_10590"
     CDS             <1..396
                     /locus_tag="PLU43_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOO28900.1"
                     /translation="RGPMESIEETLPDDFMRWDPSCHHNRALSLLEDFLHPAPYRNLL
                     LFYVWGHSFEFEREQTWTMMEEFCEKIGGKEDVWYATNIEIKEYVTAYRALISTVEGD
                     VVYNPSAVEVYLETKDGIVSVKAGEKIHL"
     gene            complement(402..>1439)
                     /locus_tag="PLU43_10595"
     CDS             complement(402..>1439)
                     /locus_tag="PLU43_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO28901.1"
                     /translation="PFETDTIEILSLILIILLLSFFTLIFAELIPQHLAAKPAESFAL
                     GVSGLVLFTSGLLTPLVWLMNAFANGILKLFGVDPKTEDEIVTEEEILMMSDAGAEKG
                     TIDEEDNKIIKNIFAFDDLTLEQICTHITDVIFLFEEKDENSWHETICNHRHRKFPIC
                     GSEIDDIIGILDVNDYFRLEDKSRENIMKNAVKEPFFVHEYMKADILLNKMKNHEAEH
                     FAVVIDEYGGVSGIITVTDLVERLIGDFSETSSNTLSEEIKKTGENEWCASGIISLGD
                     VAGVLGIELPSDTFDTLGGYLMSYASVFSKDTSVSHIDTDLLEADILKVHNHRIEKCR
                     LRIKQPAVSSD"
BASE COUNT          402 a          302 c          338 g          397 t
ORIGIN      
        1 cgtggaccca tggaaagcat agaagaaaca ttgccggatg actttatgcg ctgggatccg
       61 tcctgtcacc ataacagagc gctgtcgctt ctggaggatt tcctgcatcc ggccccatac
      121 cggaatctgc tgctttttta cgtgtgggga cacagcttcg aatttgaaag ggaacagacc
      181 tggacaatga tggaagagtt ctgtgaaaag atcggcggta aggaagatgt ctggtatgcg
      241 acaaatattg agatcaaaga gtatgtgacc gcatatcggg cccttatttc cacggtggag
      301 ggggatgttg tatacaatcc ttccgcagtg gaggtttatt tggaaacaaa agacggcatt
      361 gtctccgtaa aagcgggaga aaaaatacat ctttaaaacg atcaatcgga agaaacggcc
      421 ggctgtttga ttcgcagccg gcatttttcg atccgatgat tatggacctt aagaatatcg
      481 gcttccagca gatcggtgtc aatatgactg acagaggtat cttttgaaaa tacggatgca
      541 tatgacatca gatagccgcc aagggtatca aaggtatcgg acggcagttc gatcccgagt
      601 acgccggcca catcgccaag agagatgatc ccggaagcac accactcgtt ttcaccggtt
      661 tttttgattt cttcggaaag cgtattggag gatgtttcag aaaaatctcc gatcagccgt
      721 tcaacaagat cggtcacggt gatgattccg cttacgccgc cgtactcgtc gataacaacg
      781 gcaaagtgtt ctgcttcatg gtttttcatt ttgttgagca gaatatccgc tttcatatat
      841 tcatgtacaa aaaaaggttc ttttacagca tttttcataa tattttccct tgatttatcc
      901 tcaagccgga aataatcgtt cacatcgaga atcccgataa tatcgtcaat ttcactgccg
      961 cagatcggaa atttgcggtg tctgtgattg catatggtct cgtgccagct gttttcatct
     1021 ttttcttcaa ataaaaatat aacatctgtg atatgtgtgc agatctgttc aagtgttaaa
     1081 tcgtcaaagg caaaaatgtt tttgatgatt ttgttatctt cttcatcgat cgttcccttt
     1141 tcagcgccgg catcggacat catgaggatc tcctcttcag tgacgatttc gtcttctgtt
     1201 ttcggatcga cgccgaaaag cttcagaata ccgtttgcaa aagcattcat cagccacacg
     1261 agcggggtaa gaagtcctga agtaaaaaga acaagacctg agactccgag cgcaaatgat
     1321 tcagccggtt ttgcagcaag atgctgcggg atcagctcgg caaatatcag tgtaaaaaaa
     1381 gataaaagca atatgatcag gataagggaa agtatttcga tcgtgtctgt ctcaaaagg
//
LOCUS       DAOSYE010000690         1436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81247_length_1436_cov_3.285301, whole genome shotgun sequence.
ACCESSION   DAOSYE010000690 DAOSYE010000000
VERSION     DAOSYE010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81247_length_1436_cov_3.285301"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLU43_10600"
     CDS             complement(<1..1001)
                     /locus_tag="PLU43_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO28902.1"
                     /translation="MSEKILNKNKNGMPVMVAVIITYLLATLLCVYGGMHANTILLVI
                     CIVWLSLGWIVLLGLRILKPQEALVITLFGNYVGTLKEAGFYFINPFSTSVNPAAKTK
                     LKQSGDVDSGESKPISLSTLSQTAAIETVNKKISLKIMTLSNNKQKINDCLGNPIEIS
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRNYPYDVAPNVDTTGDGMADEG
                     SLRGSSEKVALKIKDEIQMKVQEAGLEIVEARIIYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVDMALKKLNETGMVELDEERKAAMVSNLLVVLCGNRDAQPIVNSGSL
                     "
     gene            1171..>1436
                     /locus_tag="PLU43_10605"
     CDS             1171..>1436
                     /locus_tag="PLU43_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOO28903.1"
                     /translation="MNQEKIKSVSMIGMGALGLLYGGFITEHAPDIDLKFVMDEKRYQ
                     THKNHTVLCNGIEKEFPMIPAEEAAFADLVIVAVKYNGLYDAIDL"
BASE COUNT          391 a          336 c          261 g          448 t
ORIGIN      
        1 agacttcctg aattcacgat cggctgtgca tccctgtttc cgcacagaac tactaacaga
       61 ttggatacca tggctgcttt cctctcttca tccagttcga ccattcccgt ctcattcagt
      121 tttttaagtg ccatatccac cataccgact gctccgtcca cgatcatctt ccttgcgtcg
      181 ataattgcag aagcctgctg ccgctgcagc atgacagctg cgatctccgg cgcataagcc
      241 agataaatga tccttgcttc cacgatttcc agccctgctt cctgaacctt catctgtatt
      301 tcatctttga tctttaacgc aactttttcg ctggaacctc tcaggcttcc ctcatctgcc
      361 attccgtcac ctgttgtatc aacatttggt gccacatcat acggataatt tcgtacgatg
      421 ttcctcagtg cggaatcaca ttgaagtgaa agatactctt tataattgtc cacattaaat
      481 accgctttcg ccgtatcgac aactctccat gtcactgcaa tactgatttc gattggattg
      541 cccagacaat cattgatctt ttgtttgtta ttgcttaatg tcatgatctt taacgatatt
      601 ttcttattga cggtttcgat cgctgccgtt tgggacagag tcgacaggga aataggtttg
      661 ctttctccgc tgtccacatc cccgctttgc ttcagttttg ttttggccgc agggtttaca
      721 cttgtactga acggattaat gaaataaaac ccggcttcct tcagtgtgcc gacataattg
      781 ccgaacagtg taatgaccag cgcttcctgc ggttttaaaa tgcgcagtcc taaaagtacg
      841 atccatccaa ggctcagcca cacaatacaa ataacaagca aaattgtatt ggcatgcatt
      901 cctccgtaaa cacacagcag ggtcgccagc agatatgtaa tgatcacagc aaccatgacc
      961 ggcattccgt ttttgttttt attcaatatt ttttcactca tcccgattac ctccatatgg
     1021 taaatatcta tttgatatca ttatgatatc accttgtatt ttatttgtca atttccccag
     1081 cattattttt ttgatttccg caaggtagta tatttaccga aaaaatgata tagtaataaa
     1141 ataaacggac agaaaggaac acctatattt atgaatcagg aaaaaattaa atcggtcagt
     1201 atgatcggca tgggagcatt aggacttctt tacggaggat ttataacgga acatgcgccg
     1261 gatattgatt tgaaatttgt aatggatgaa aaaagatatc aaacacataa aaatcatacg
     1321 gtactgtgta acggaatcga aaaagaattc cccatgattc cggcagaaga agctgctttt
     1381 gcagatcttg ttattgtagc cgtaaaatat aacgggctct atgatgccat tgacct
//
LOCUS       DAOSYE010000691         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81491_length_1434_cov_2.957941, whole genome shotgun sequence.
ACCESSION   DAOSYE010000691 DAOSYE010000000
VERSION     DAOSYE010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81491_length_1434_cov_2.957941"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLU43_10610"
     CDS             complement(<1..661)
                     /locus_tag="PLU43_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOO28904.1"
                     /translation="MTNFEKGVKSFAIALAVILCIAILGAIASAVGGVVNGIRHGAAA
                     VSEAAETASEAISDSAQQVHSDLYNEVAEQLPESSEYYFSTDDVEDLSIQNGIGDMTI
                     RETDDSRIHVTLGSTTECCSVGLSGDTLKIENSGVDVEIFGIRIGEKVENGEGGMVVE
                     VPKGFAFEDVSVENGVGNMDISGIYAEDITISNGTGDLEIDRIDAEKIKVESGIGEIG
                     IN"
     gene            complement(658..>1434)
                     /locus_tag="PLU43_10615"
     CDS             complement(658..>1434)
                     /locus_tag="PLU43_10615"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOO28905.1"
                     /translation="MNNRNRVGSILWGVVFILVGIGIAGNAFHIWNFELFFKGWWTLF
                     IIVPCAISIVQNGPRSGSIIGLVIGILLFLMQQNGIGWSTIGDLIVPLILVAIGLGFI
                     LRSNNRKSRHTYENDERFCDCQNAGSYTAEENCTQSSNFKEESNSYTGGKVKDISAIF
                     GSRKVTYQNETFTGASINAIFGGACLDLRNASMSGNIEISASAIFGGIDILVPGNVCV
                     VVNSVPIFGGVSNKAMSPVGDVKASIHVNATCMFGGIDIK"
BASE COUNT          406 a          397 c          245 g          386 t
ORIGIN      
        1 aatttattcc aatctcaccg attcctgatt ccacttttat tttttccgca tcgatccgat
       61 cgatctccag atcaccggta ccgttcgata tcgtgatatc ttccgcataa attccggaaa
      121 tatccatatt gccaacaccg ttctcaacag atacgtcttc aaaagcaaac cctttcggaa
      181 cttcaacgac catgccgcct tctccatttt caaccttttc tccgatccgg ataccaaata
      241 tttccacatc cacgccggaa ttttctattt tcagcgtatc tccggaaagt ccgacgctgc
      301 agcactccgt cgtactgccg agtgtcacgt ggatccggga atcatcagtc tctctgatcg
      361 tcatgtcacc gatcccattc tgaatcgaca gatcctccac atcatcagtg gaaaagtagt
      421 attcagaaga ttccggaagc tgttcggcaa cttcattata caaatccgaa tgaacctgct
      481 gcgcagaatc agagatcgct tcggaagcag tttccgcagc ttccgaaaca gcagcggctc
      541 catgccggat tccatttacg accccgccga cggcactggc aattgcaccc aaaatcgcta
      601 tgcacaatat aacagccaat gcaatggcaa atgactttac gcccttttcg aaatttgtca
      661 tttaatatca attcctccaa acatacaggt tgcatttaca tggatcgacg ctttgacatc
      721 cccaacagga gacattgctt tattggatac accgccgaag atcggaacac tgtttaccac
      781 aacacagaca ttccccggga ccagaatatc gatccctccg aagatcgcag atgcggaaat
      841 ttctatattt ccgctcattg acgcattgcg gagatccaga caggctccgc caaagatcgc
      901 attgatcgac gcacccgtaa atgtttcatt ttgataagtt acttttctgg agccaaaaat
      961 cgccgaaata tcctttactt ttccgcccgt gtagctgttt gactcttctt taaaattcga
     1021 ggactgtgta caattttctt ccgcagtgta tgaccctgcg ttctgacaat cacaaaatct
     1081 ttcatcgttc tcatacgtat gccttgactt tctgttattg cttcttaaaa taaatccaag
     1141 accgatcgca acaagaataa gcggaacgat cagatcaccg atcgtactcc agccgattcc
     1201 gttctgctgc ataaggaata gcaaaatacc aattacaagg ccgataatac tccctgaacg
     1261 cggaccgttc tgaacaatgg aaattgcaca gggaacaata ataaacagcg tccaccagcc
     1321 tttaaaaaac agttcaaaat tccatatatg aaacgcattg ccggcaattc cgattcctac
     1381 cagtataaac acaacacccc ataaaatgct tcctaccctg tttctgttat tcat
//
LOCUS       DAOSYE010000692         1432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81696_length_1432_cov_1.949891, whole genome shotgun sequence.
ACCESSION   DAOSYE010000692 DAOSYE010000000
VERSION     DAOSYE010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81696_length_1432_cov_1.949891"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLU43_10620"
     CDS             <1..434
                     /locus_tag="PLU43_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28906.1"
                     /translation="LNANLAKEKERIVQLLSINASNAELIRRYKSEITTMRDIMKSYI
                     VQIDSLNTRNKILVAENQQIKQEISKVQNTNEELEKVKAELNAQVEVASVIQAKNIIP
                     VALNKKNKETSKLSLLSIVRVCFTLMRTMKYSAGLRLPLKP"
     gene            371..>1432
                     /locus_tag="PLU43_10625"
     CDS             371..>1432
                     /locus_tag="PLU43_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO28907.1"
                     /translation="MFYFDENNEVQCGLATSFEAVDTTTYVMGIRDDVYFSTGEPMTV
                     DDVIYSLDRMRNPDNASKLLFMFDYVDSIEQTGDWEVTIKLTQGDANFQYALATSAGL
                     VISKKWAEEIGEENIGSVEDGIIGTGPYVYGSRTVGSEVVLEKNENWWNKDASVDIDE
                     VRIEIIPDVNSRALALKSGQIDYTPSVSESIMEEVMSYDNITYNHQPGYTNKFITFNT
                     EHTPLDNKLVRQAICCSVDWDAIYESLYSEVGEKCGDKFWGAASDITDAFDSYTPDNR
                     KYTYDVEKAKELLTEAGYPDGFETTISYRTNDPKNETFALAMQQYMSEVGITATLVSY
                     SYPEFQPLHYGYTLAEDGTR"
BASE COUNT          468 a          246 c          340 g          378 t
ORIGIN      
        1 ctcttaacgc aaatctcgcc aaagaaaagg aacgtattgt tcaactgtta tctataaatg
       61 cttccaacgc cgagcttatc agaagatata aaagcgagat aacaaccatg agagatatta
      121 tgaaaagtta tatcgtccaa atagactctt taaacacccg caacaaaata ttggtcgccg
      181 agaaccaaca gataaaacag gaaatttcaa aagttcaaaa tacaaatgaa gagcttgaaa
      241 aggtgaaagc tgaactcaac gctcaagttg aagtcgcttc ggttatacag gcaaagaata
      301 taattccggt tgctttaaac aaaaaaaata aagagacatc aaaattgagc ttattaagca
      361 ttgtgagggt ttgttttact ttgatgagaa caatgaagta cagtgcgggc ttgcgacttc
      421 ctttgaagcc gtagatacta caacttatgt tatgggtatc agggatgatg tgtatttttc
      481 aacaggagag cctatgacgg tagatgatgt catttattcc ctggatcgta tgagaaatcc
      541 tgataatgca tccaagctgt tatttatgtt tgattatgta gattcgatcg aacagacagg
      601 tgattgggaa gtcacaatca aattgacaca gggggatgct aatttccagt atgcgttagc
      661 taccagtgca ggcctggtta tttcaaagaa atgggctgaa gaaatcgggg aagaaaatat
      721 tggttccgta gaagacggca ttataggaac aggtccgtat gtatatggaa gcagaacagt
      781 cggttcggaa gttgtactgg agaaaaatga aaactggtgg aacaaggatg cgtccgtaga
      841 tattgacgag gtcaggatcg agatcattcc ggatgttaat tcacgtgcgc tggcgctgaa
      901 gtccggtcag atagattaca cgccaagtgt ttccgaatcc attatggaag aagtgatgtc
      961 atatgataat atcacttata accatcagcc cggatatacg aataagttca taacctttaa
     1021 tacagaacat acgccgttgg ataacaagct ggtaaggcag gcgatctgct gttctgtcga
     1081 ctgggatgcg atttatgaaa gcttatattc agaggttggt gaaaagtgcg gagataagtt
     1141 ctggggtgct gcttcagata ttacagatgc atttgattcc tatacgccgg ataacagaaa
     1201 gtatacgtat gatgtggaaa aggcaaagga attattgact gaggcaggtt atccggatgg
     1261 ctttgaaact acgatttctt atcgtacaaa tgatcctaaa aatgaaacct ttgcattagc
     1321 tatgcagcag tacatgtcgg aagtcggcat aacggcaact ttggtcagct attcttatcc
     1381 ggaattccag ccgctgcatt acggctacac attggcggag gacgggacaa gg
//
LOCUS       DAOSYE010000693         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81727_length_1431_cov_4.002180, whole genome shotgun sequence.
ACCESSION   DAOSYE010000693 DAOSYE010000000
VERSION     DAOSYE010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81727_length_1431_cov_4.002180"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLU43_10630"
     CDS             complement(<1..216)
                     /locus_tag="PLU43_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOO28908.1"
                     /translation="MNIYDFHVTARDGNEISLSQYKGKVLLIVNTATACGFTPQYEEL
                     QALYETYGPKGLEILDFPCNQFGEQAPG"
     gene            381..>1431
                     /locus_tag="PLU43_10635"
     CDS             381..>1431
                     /locus_tag="PLU43_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HOO28909.1"
                     /translation="MSIRIYTMTHKKFEVPPDDMYVPLQVGCADAKKLGYLEDNTGDH
                     ISELNCYYSELTGIYWIWKNIKDIDYVGTCHYRRYLINRQEYFFGRQELEQLLAEYDL
                     ITTKKVQLLNSYYNGFSANHNKAVLDETGRVIRETYPEYYETFVRLVNGKETYFGNIM
                     ICKKTLFDEYADWLFTIFFEVQKRVDLTAEDQYHKRVFGFISEFLLYVFAAVRQLKVY
                     ECKVGMLGEKAETRELKEQLAGYFKRKDVEGAKKYFLSIYKIRPDVLMEASDITGELK
                     LSMQVIATCEQEQIYEQSCILDRITDFSALMHYFSMVNKIAQKKQEQEPYYIEELQFI
                     KTNEISETAMKIAELL"
BASE COUNT          474 a          230 c          315 g          412 t
ORIGIN      
        1 tcccggagcc tgctctccga attggttaca tggaaaatcc agtatttcca gtcccttcgg
       61 tccataggtt tcatatagag cctgcagctc ttcatattga ggtgtaaatc cgcaggcagt
      121 agctgtattg acgatcagca gcacctttcc tttgtattgg gaaagagata tttcattgcc
      181 gtcccttgcc gttacatgaa agtcataaat attcataagt attcctcctt tgttatattg
      241 gatgtatgtt cgcccataag attttggaca attagattgt acacaattta aaaagagatg
      301 tcaacaggta atttgaatta atggtttttt atttattcat ttcatgatat atttattata
      361 ataaaaaagg aacgaaaaca atgtctatca gaatttatac aatgacgcat aaaaaattcg
      421 aagtcccgcc ggatgatatg tatgttccgc tgcaggtcgg atgcgcagat gcaaaaaagc
      481 ttggatatct ggaagataat accggagatc acatatcgga actaaactgt tattacagtg
      541 aactgaccgg catctattgg atatggaaaa atataaagga tattgattat gtcggaacct
      601 gccattacag aagatatctg atcaacaggc aggaatattt ttttggcaga caggaactgg
      661 aacagctgct ggccgaatat gatttgatca caacaaaaaa agtacagctg ctaaattctt
      721 attataatgg gttttctgcc aatcataata aagcggtgct tgatgagaca ggaagggtga
      781 tcagggaaac ttatccggaa tattatgaga cctttgtcag gctcgttaac ggaaaagaaa
      841 cgtatttcgg caatattatg atctgtaaaa agaccttgtt cgatgaatat gcagactggc
      901 tgtttaccat tttttttgag gtgcagaagc gggtagatct cacggctgaa gatcagtatc
      961 ataaaagggt atttggattt atttcggagt ttcttttata tgttttcgcc gcagtaaggc
     1021 agctgaaggt ttatgaatgc aaagtaggga tgcttggaga aaaagcggaa acaagagaac
     1081 tgaaagaaca gcttgccgga tattttaaaa gaaaagatgt ggaaggtgca aaaaaatatt
     1141 tcttaagtat ttataaaata cggccggacg ttctgatgga agcttcagat ataacggggg
     1201 agttaaaact gtcaatgcag gtcattgcaa catgcgagca ggaacaaatc tatgaacagt
     1261 cctgtattct ggacagaatc acggactttt ctgcgttgat gcattatttc agcatggtta
     1321 acaaaattgc acagaaaaag caggagcagg agccgtatta tatagaagag ctgcagttca
     1381 tcaaaacaaa tgaaatatca gaaactgcga tgaaaatagc agaattattg t
//
LOCUS       DAOSYE010000694         1429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_81945_length_1429_cov_3.306405, whole genome shotgun sequence.
ACCESSION   DAOSYE010000694 DAOSYE010000000
VERSION     DAOSYE010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81945_length_1429_cov_3.306405"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLU43_10640"
     CDS             complement(<1..1147)
                     /locus_tag="PLU43_10640"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOO28910.1"
                     /translation="MKFAEAVRYIEGAGKYGSKLGLTRMRDLMEQLDNPQDELRFIHI
                     AGTNGKGSVLAYIASVLKASGYKTGSYSSPAVENFREHFQINGVMISKKQYAKCVEKL
                     QAAAERMQEAGKQCPTLFELETALGFLYFKENRCDVVVLETGLGGSLDATNIIGTTVL
                     SVITSISMDHAELLGNTLEEIAEQKAGIIKDHVPVVMLENTECITNVIKRKCRITSSE
                     LFIAKEEKLRDVVFGFPAQKFSYYQFANARISLGGIHQIMNAALAIEAFWSLRNSDFF
                     IPAAKIREGLKAARWPYRMEQILKKPQFIVDGAHNPDAAQRLADSLQLYFGERTLIFI
                     IGVFKDKDYQKICEVTAGMARHIITVQTPDSARALSAEALQAAVKKYN"
BASE COUNT          361 a          380 c          251 g          437 t
ORIGIN      
        1 gattatattt cttcacagcc gcctgcagag cttctgccga aagagccctg gctgaatccg
       61 gcgtctgcac agtaatgata tgtcttgcca taccggctgt gacctcacag atcttttgat
      121 aatccttgtc tttaaaaacc ccgataataa agatcagtgt ccgctcccca aaatacagct
      181 gcaatgaatc cgcaagcctc tgtgctgcat cgggattatg tgccccgtct acaataaact
      241 gcggcttctt taaaatctgt tccatcctgt acggccacct ggctgccttc aggccttccc
      301 ttatttttgc cgccggaata aaaaaatcgg aatttctcag actccagaac gcctcgattg
      361 caagtgccgc attcatgatc tgatgaattc ctccaagcga aatccgtgcg ttggcaaatt
      421 gataatagct gaacttctgt gccggaaagc cgaaaaccac atcccgcagc ttttcttctt
      481 ttgctataaa cagttctgaa cttgtaattc tgcattttct tttaataaca tttgttatgc
      541 attccgtatt ttcgagcatt acgaccggca catggtcctt tatgatccca gccttctgct
      601 ccgcgatctc ttcaagcgta tttccaagca gctccgcatg atccatgctg atcgaggtga
      661 tcactgataa aactgtcgta ccaataatat tcgttgcgtc cagacttcct ccaagaccgg
      721 tctccagcac aacgacatca catctgtttt ctttaaaata aagaaagccc agtgctgttt
      781 ccagctcaaa aagcgtggga cactgcttcc ctgcttcctg cattctttct gcggcagcct
      841 gtaatttttc gacacatttt gcgtactgtt tcttgctgat catgacaccg tttatctgaa
      901 aatgctcacg gaaattctca acagcaggtg aggaatagct tccggtttta tatccggatg
      961 cttttaatac ggacgctata tacgcaagta ccgagccttt tccgtttgtt cctgcaatat
     1021 gaataaatcg cagctcatcc tgcggattat caagctgttc catcaggtcc cgcatgcgcg
     1081 taagccccag tttgctcccg tatttacctg cgccttcaat ataccggacc gcttctgcaa
     1141 atttcatccg tatttcccct tacttctgct taatcagtct ttatcatcat ataacaactt
     1201 agataaattt acaataaatg aatcatgtca ttacataata agcactaata taaaaacgca
     1261 ctggccgtaa aaccaatgcg ctttcctgtt actgtaaatc aacatcgtcg atcgactcca
     1321 gcccctcggt aagattcagc gtattcagcg ttcttctttt ccttcgcatt tcctccgatt
     1381 gttctaaaac aaaatcttta actgctttgg aatttttaat gtgctccag
//
LOCUS       DAOSYE010000695         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_83542_length_1413_cov_3.439617, whole genome shotgun sequence.
ACCESSION   DAOSYE010000695 DAOSYE010000000
VERSION     DAOSYE010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83542_length_1413_cov_3.439617"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..833)
                     /locus_tag="PLU43_10645"
     CDS             complement(138..833)
                     /locus_tag="PLU43_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO28911.1"
                     /translation="MANKQRILIVDDDANIAELISLYLTKECFDTNIVHDGEEALTAF
                     ETFQPNLILLDLMLPGIDGYQVCREIRAKSTTPIIMLSAKGEIFDKVLGLELGADDYM
                     EKPFDSKELVARVKAVLRRIAPAKPAVVSDTKCVEYPDLIINQTNYSVLYYGEPVEMP
                     PKELELLYFLASSPNQVFTREQLLDHIWGYEYIGDTRTVDVHIKRLREKIKDHDAWRL
                     STVWGIGYIFEVK"
     gene            complement(876..>1413)
                     /locus_tag="PLU43_10650"
     CDS             complement(876..>1413)
                     /locus_tag="PLU43_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOO28912.1"
                     /translation="RLEKTQNSDLIQKRTPQKQHKPADFRLGERVRILSMGLTGTVNS
                     LPDAKGNLFVQCGIMRMQAKMNDVEFLPSEDITGPGLSGRKKQSGKSGLSKSGSISPE
                     INLLGLTADEAISKLDKYLDDAYLSHLSSVRVVHGKGTGALRTAIHTYLKKIQYVKSY
                     KLAEYGEGDAGVTIVEFK"
BASE COUNT          351 a          326 c          323 g          413 t
ORIGIN      
        1 ggcagcggcc ttatctttga tcaggaaatc cagagtcata tccgaagtca gtgttgcaat
       61 ggcaataaag cttaaaaatc cgaatacgat atatgcaatt atgaacttca gataaagtgt
      121 ctgcttggtt ttcatatcta tttcacctca aatatgtatc cgatccccca tacggtagat
      181 aaccgccatg cgtcatgatc tttgatcttt tcacgaagcc gtttgatatg tacgtcaaca
      241 gtcctcgtat caccgatata ttcatagccc cagatatgat caagaagctg ttctcttgta
      301 aatacctgat tgggcgaaga ggcaagaaaa tagagaagct ccagttcctt tggcggcatc
      361 tcgaccggtt cgccgtagta caggacagag taatttgtct ggttgatgat cagatccgga
      421 tattccacac atttcgtatc ggaaacaacg gccggctttg cgggagcgat ccgccgcaga
      481 acagctttca cacgtgcgac aagttctttg gagtcaaacg gtttttccat ataatcgtcc
      541 gcaccgagct cgagtcccag tactttatca aaaatttctc cctttgcgga aagcataata
      601 atgggcgtcg tggatttggc acggatctcc cggcagacct gatatccgtc gatcccgggc
      661 agcatcagat ccagtaaaat caaattgggc tgaaatgttt caaaagcggt cagagcctct
      721 tccccgtcgt gcacaatgtt tgtgtcaaag cattcctttg tgagatacag ggaaatcaat
      781 tcggcgatat ttgcatcgtc atctacaata agtatccgtt gtttgtttgc catgaagaac
      841 cctcctgatt gatctttata gtgtttgttg aacagttatt tgaattcgac gatcgtaaca
      901 ccggcatcgc cttctccgta ttctgcaagt ttataggatt ttacatactg tatttttttc
      961 agatacgtat ggatggcagt gcgcagggcg ccggtgcctt ttccgtgaac gacacggaca
     1021 ctggacaaat gagacagata tgcgtcgtcc agatatttgt caagctttga aattgcttca
     1081 tctgccgtca gtccaagcag attgatctcc ggggagatcg aaccggattt tgaaagaccg
     1141 ctttttccgg actgtttttt tctgccggaa agaccaggcc ccgtaatgtc ttctgagggg
     1201 agaaattcaa cgtcgttcat ttttgcctgc attctcatga tgccgcattg tacgaataag
     1261 tttccttttg cgtcaggaag ggaattaacc gtaccggtca gtcccatgga caggatcctg
     1321 acacgttccc caagccggaa atccgccggc ttatgttgtt tttggggtgt gcgtttctgt
     1381 ataagatcgc tgttctgcgt tttttccagc cgg
//
LOCUS       DAOSYE010000696         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84109_length_1407_cov_2.888314, whole genome shotgun sequence.
ACCESSION   DAOSYE010000696 DAOSYE010000000
VERSION     DAOSYE010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84109_length_1407_cov_2.888314"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLU43_10655"
     CDS             complement(<1..289)
                     /locus_tag="PLU43_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOO28913.1"
                     /translation="MIEIGKYNTLEILRHTSVGLYLGDESGEDVLLPNKYCPEEYEIG
                     GKIEVFVYRDYAERKIATNIDPKIKLHEFAFLQVNAVSEVGAFLDWGLEKDL"
     gene            438..>1407
                     /locus_tag="PLU43_10660"
     CDS             438..>1407
                     /locus_tag="PLU43_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOO28914.1"
                     /translation="MQSFNLVAKTLYGLEEVLARELEMIGAQEIKPLNRAVMYRGDLE
                     VMYKSNLLLRTAQKILRPIRTFEVQNENQLYKKLQQINWPGFFHVDQTFAVDGTTQGN
                     IFTHSKYVALRTKDAIVDQFRDRLGKRPSVDIDHPDIRINIHISDMRCTVSMDSSGVQ
                     LGRRGYRAMQVEAPISETLAAGIILLSGWKGQKPFLDPMCGSGTFPIEAAMIAANIPP
                     GRMHEFAFENWKDYDDKLWKRIQKEVDSYIKPIDVDIRGMDMDADAVKIASQNAKYAG
                     VDKWVRFGVNNFLENTPEMKPGTIIVNPPYGERLKEKDEIVPFYQEI"
BASE COUNT          447 a          276 c          299 g          385 t
ORIGIN      
        1 gaagatcttt ttccaatccc cagtcaagga atgcacctac ttctgaaaca gcatttacct
       61 gcaaaaaagc aaattcatgc aatttgattt taggatctat attggtagcg atttttcttt
      121 cggcataatc acgataaaca aaaacttcaa tcttccctcc tatctcatat tcttcaggac
      181 agtatttatt tggaagaagt acatcttcac cggattcatc accgaggtac aggccaacgc
      241 ttgtgtggcg cagtatttcc agtgtattgt attttcctat ctctatcaca gtataatttt
      301 ctacaaagat agagaatcaa tggaatattt cgtttcaata aacattaata aaaaacagtc
      361 tgacaactat ttgtatcttt gcagcgcttt tgatatcaca aggaatatct tcgcattaat
      421 cataaaaaaa taaggcattg caatcattca atttagtagc taaaacactt tatggactgg
      481 aagaggtact ggcccgtgaa ttagagatga tcggagccca agagattaaa cctctaaaca
      541 gggcagtaat gtatcgtggc gatctggagg taatgtataa gagcaatctt ttgctccgca
      601 cggcacagaa gatcctgcgt ccaatccgta cttttgaagt acaaaatgag aatcagttat
      661 ataaaaagct gcaacagata aactggccag gattttttca tgtggatcag accttcgcag
      721 ttgatggcac cacgcagggg aatattttta cacattcaaa atatgttgct ttacgaacga
      781 aagatgccat tgtggatcag tttcgcgacc gtttaggcaa acgaccgtct gtggatattg
      841 atcaccctga cattcggata aacattcaca tttcagacat gcgatgtacg gtatccatgg
      901 acagtagcgg cgtacagctt ggacgccgcg gatacagggc aatgcaggta gaggctccga
      961 tttcagaaac ccttgccgcc ggtattatac tattgtcagg atggaaaggt caaaagccat
     1021 ttcttgaccc tatgtgcgga tcaggaacat tccctattga agcagctatg attgcggcca
     1081 acattcctcc cgggagaatg catgaattcg ctttcgagaa ctggaaggat tatgatgaca
     1141 aactctggaa aagaatacaa aaagaagtag acagctatat caagcctatt gatgttgata
     1201 tccgagggat ggatatggat gcagatgcag taaaaatagc cagccagaat gcaaaatacg
     1261 ccggcgttga taaatgggtt cgttttggtg taaataattt tctggagaat acaccggaaa
     1321 tgaagccggg aacgatcatt gtcaatccac cttacgggga gcgattaaag gagaaagatg
     1381 aaatagttcc tttttaccag gaaatcg
//
LOCUS       DAOSYE010000697         1404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84412_length_1404_cov_2.824314, whole genome shotgun sequence.
ACCESSION   DAOSYE010000697 DAOSYE010000000
VERSION     DAOSYE010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84412_length_1404_cov_2.824314"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>973)
                     /locus_tag="PLU43_10665"
     CDS             complement(<1..>973)
                     /locus_tag="PLU43_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO28915.1"
                     /translation="GTSDAGALRAANGHAAPYKVDYFGVGNESWGGGGNMTAAYYADE
                     YRRYQTYLKNYNKAVDMKLVAVGAPDDRKEWTEQVLCEAYKEAPADTHGFMDGLAVHY
                     YTVPGTWEKKGSATDFTEDEWYGTMRKTMEMDRIIRLHGKIMDKYDPEKKIAMVIDEW
                     GTWLDPEPGTNPAFLYQQNTMRDALVAGINLNLFNKNCDRVKMANIAQTINVLQAVIL
                     TDGKEMLLTPTYHAFDLYKVHQENTLLESSVEQSVIGTETDHVVNLTESVSEDENGTI
                     YITITNQSLSEAYDIEAIFADYPVGEVHGRILTGEMNAHNTFSNKDHV"
     assembly_gap    974..983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<984..1373)
                     /locus_tag="PLU43_10670"
     CDS             complement(<984..1373)
                     /locus_tag="PLU43_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOO28916.1"
                     /translation="MAKLFINPHRKMGHINKELQGHFSEHLGRCIYDGIFVDEQQEIP
                     NIDGMRTDIVDALKHIQIPVLRWPGGCFADIYHWRKGIGPKESRGKTVNSLWGDVIEN
                     NHFGTDEYMNLCDQLGCKTYISGNLGSG"
BASE COUNT          318 a          391 c          252 g          433 t
ORIGIN      
        1 tcacatggtc tttattagaa aacgtattat gtgcattcat ctcacctgtt aaaatcctgc
       61 catggacttc cccgaccgga taatccgcaa agatcgcttc tatgtcataa gcttcgctta
      121 acgactgatt tgtgatcgtt atataaatag ttccgttctc atcttccgat accgattccg
      181 tcaggtttac gacatgatca gtttcagttc cgatgacaga ttgctctacg ctactttcaa
      241 gcagtgtatt ttcctgatgc actttataaa gatcgaatgc atgataagtc ggtgtcagaa
      301 gcatttcttt tccgtccgtc agtatgactg cctgcagcac attgatcgtc tgtgcaatat
      361 tagccatttt taccctgtcg cagtttttat tgaacagatt tagattgatg cctgccacaa
      421 gtgcatcccg catcgtattc tgctgataaa gaaatgccgg attcgtgccg ggttccggat
      481 ccagccacgt cccccattca tctatcacca tcgcaatttt cttttccgga tcgtacttgt
      541 ccatgatttt cccgtgcagc ctgatgatcc ggtccatttc catcgttttg cgcatcgtgc
      601 cataccattc gtcttctgta aaatcagttg cgcttccctt tttttcccac gtccccggaa
      661 ctgtataata atgcactgcc agcccgtcca taaacccatg cgtatctgcc ggcgcctcct
      721 tataggcctc acacaatacc tgttcggtcc attccttcct gtcatccggt gcgcctacgg
      781 caaccagctt catatcgaca gccttgttgt aatttttaag atatgtctga tatctcctgt
      841 attcatcagc atagtaggca gccgtcatat ttccgccgcc tccccagctc tcattaccga
      901 caccaaaata atctactttg taaggtgctg catgtccgtt tgcagcacgg agggcacctg
      961 catcagaagt tccnnnnnnn nnngccgctt ccaagatttc cggaaatata cgtcttgcag
     1021 ccaagctgat cgcataaatt catatattca tctgttccga aatgattatt ctctatcaca
     1081 tcgccccaga gggaattgac cgtctttcct cgcgactcct ttggcccgat cccttttctc
     1141 cagtgataaa tatcggcaaa gcatccgccc ggccaccgca gtaccggaat ctgaatatgt
     1201 ttcagcgcgt ctacgatatc tgtcctcatg ccgtcaatat tcggaatttc ctgctgttca
     1261 tccacaaaga tgccgtcata aatacacctg cccagatgct cgctgaaatg tccctgcagc
     1321 tctttattga tatgtcccat tttcctgtgt ggatttataa ataatttagc cataatctgt
     1381 ctacatcagc ctttcacacc gcct
//
LOCUS       DAOSYE010000698         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84608_length_1402_cov_3.272457, whole genome shotgun sequence.
ACCESSION   DAOSYE010000698 DAOSYE010000000
VERSION     DAOSYE010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84608_length_1402_cov_3.272457"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLU43_10675"
     CDS             complement(<1..647)
                     /locus_tag="PLU43_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO28917.1"
                     /translation="MSDIQEKYTLGIDIGSTTVKIAVLDSANAILFAEYERHYANIKD
                     TLKGLLSQAYEKLGDLTVHPMITGSGGLTLANHLGVPFVQEVIAVSTALQDYESKTDV
                     AIELGGEDAKIIYFEGGNVEQRMNGICAGGTGSFIDQMASLLQTDAAGLNEYAKGYRS
                     LYTIAARCGVFAKSDIQPLINEGATKEDLSASIFQAVVNQTISGLACGKPIRGHVA"
     gene            909..>1402
                     /locus_tag="PLU43_10680"
     CDS             909..>1402
                     /locus_tag="PLU43_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28918.1"
                     /translation="MNVSMNNLLNKMERKIGRYAIHNLSLYIIIGYAVGYILNLTGSL
                     EFLSLDPYRILHGQIWRLVTWILVPPSSLGIFTIIMLFFYYSIGTSLERTWGAFRYNV
                     YIFSGMLFTLIGAFILYAIYTQFGGYMGEEALIGQLVSQKFSTYYVNLSIFLTFAACY
                     PNMQV"
BASE COUNT          390 a          302 c          292 g          418 t
ORIGIN      
        1 gcaacatgac cgcggatcgg cttgccgcat gcaagtccgc tgatcgtctg gttgacgacg
       61 gcctgaaaaa tagaagcgga cagatcctcc tttgttgcac cctcgttgat cagaggctga
      121 atatcggatt ttgcaaagac accgcatctg gcggcaattg tatacaaaga acggtatcct
      181 tttgcatact cgttcaaacc tgcagcatct gtctgcagca gggaagccat ttggtcgata
      241 aaagagccgg tcccgcccgc acagatacca ttcatgcgct gttcgacatt cccgccctca
      301 aaataaatga tcttggcatc ttccccgccc agttcgatgg caacgtcagt tttgctctca
      361 taatcctgca gtgcggtgga gacggcaatg acctcctgca cgaaaggaac gccaagatga
      421 ttggccagcg tcagtccgcc agagcccgta atcattgggt gaacagtcag atcaccgagc
      481 ttttcatatg cctgtgacaa taaccccttt aatgtatctt tgatatttgc ataatgacgt
      541 tcgtattcgg caaacaatat ggcatttgcc gaatccagta cggcaatttt taccgtagtg
      601 gaaccgatat cgatcccgag cgtatatttt tcctgaatgt ctgacataat gacacccttt
      661 cttcttattt aaatattgtt ttgaaaccat aatcaaagta cattatacga catcatttta
      721 taaagtgcaa tgaaatatcc cttctgaaaa atgtaaaaac tttattaaga atcaatagaa
      781 acaaatgaaa tttttggcat gacggtttac ttttagtaat tggaatgata aactttacat
      841 gttaatcata tcgaaccatg ccttgcatac agatcaaaca gaagtttata ataataaagg
      901 agttttttat gaatgtttct atgaacaatt tattaaacaa aatggaaaga aaaataggaa
      961 gatatgcgat tcacaactta tccctctata tcattatcgg atatgcggtc gggtatattc
     1021 tgaacttgac aggctcgctt gaatttctca gtctggatcc ataccggata ctgcacggac
     1081 agatctggcg gcttgtgaca tggatccttg taccgccgtc cagtcttggc atcttcacga
     1141 tcattatgct gtttttctat tattcgatcg gtacatcctt agagagaaca tggggggctt
     1201 tccggtataa tgtctatatt ttttcgggga tgctatttac gctgatcggt gcatttattt
     1261 tgtatgcgat ctatacacag ttcggcggat atatgggaga agaagcactg atcggtcagc
     1321 tcgtcagtca gaagttcagc acttattatg tcaatttgtc gatctttctg acgtttgcag
     1381 cctgttatcc gaatatgcag gt
//
LOCUS       DAOSYE010000699         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84657_length_1402_cov_2.230141, whole genome shotgun sequence.
ACCESSION   DAOSYE010000699 DAOSYE010000000
VERSION     DAOSYE010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84657_length_1402_cov_2.230141"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..807)
                     /locus_tag="PLU43_10685"
     CDS             complement(169..807)
                     /locus_tag="PLU43_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28919.1"
                     /translation="MKDPLFKKRLFMALSGVLICGISVGLFRRSVFGTDPFQVLVNGL
                     SNQITVLDFGTLYMIINLLMLAAVFFLDKHYIGVATFINIFLLGYVVQFSDDCIGRLL
                     GDSLNLVTRIILLTAGIIIMCFASSLYFTADLGVSTYDSISLIMTDKKLGKFKYLRIG
                     TDLFCVIVGFILGGIVGIGTVVTAFFMGPLIDFFNKKAARPFLYGKNSTVQN"
     gene            1030..>1402
                     /locus_tag="PLU43_10690"
     CDS             1030..>1402
                     /locus_tag="PLU43_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO28920.1"
                     /translation="MEEIRYEDAAGLDERILRAVKELGFEVMTPIQAQAIPLMMNGQD
                     IIGQAQTGTGKTAAFAIPALQNAVPGEGLQCLILCPTRELAIQAAEEIRKLGKYMHGV
                     RVLPIYGGQDISKQIRSLKGNV"
BASE COUNT          456 a          272 c          357 g          317 t
ORIGIN      
        1 tatcacgcac ttcatcatca ttaaactttg ctaatacaaa tgagcaagct ttttccggaa
       61 caggaagcat ccgtaagatt tcggatgtgc ggacacgggg aaatgtatgt gtactgcaac
      121 agacacggag cctataggaa aagaatatag gctccgcata aaatgaaatc aattctgcac
      181 ggtactgttt tttccgtata aaaacggccg ggctgctttt ttattgaaga aatcaatcag
      241 aggacccata aagaaagcgg taactaccgt accgatcccg acgatcccgc caagtataaa
      301 tccgacgata acacagaaca ggtcggtgcc gatacgcaga tatttgaatt ttccgagttt
      361 tttatcggtc atgatcaggg atatggaatc ataagtcgag acaccaagat cggccgtaaa
      421 gtaaagggaa gaagcaaaac acataataat gatgcctgct gtcaaaagga tgatacgggt
      481 gacgagattg agtgagtcgc caagaagccg gccgatacag tcgtcggaaa actggacgac
      541 atagccgagc agaaagatat tgataaaggt agcgactcca atataatgtt tatctaagaa
      601 aaaaacggcg gccagcatca gcagattgat gatcatatac agcgtaccaa agtcaaggac
      661 cgtgatctgg ttgctgagac cgttgaccag tacctggaac ggatccgtac caaagacgga
      721 gcgccggaac agaccaacgg aaataccgca gatcagaaca ccggacagtg ccataaacag
      781 gcgttttttg aatagtggat ctttcatttg cattctcctt tttttaaact gtatctattg
      841 tagggcaaac aagagggcag gtcaagaaga attaacagtt gacaagggac ctttcttcgg
      901 gtaatttaat agaagtgtgt taatattgta atgatgttca ggtacacgga ggtgtacttg
      961 ttttttatat aaataaagaa cacatattaa tttacagaat gttattataa ggaaacgaaa
     1021 ggaaacgaaa tggaagaaat cagatatgaa gatgcggctg ggctggacga gaggatcctg
     1081 cgtgccgtaa aggaattggg gtttgaagta atgacgccca tacaggcaca ggcgattcct
     1141 ctgatgatga atggacagga cattatcgga caggcgcaga caggaaccgg aaagacggcg
     1201 gcatttgcca ttcctgcctt acagaatgca gtaccggggg aaggcttgca gtgcctgatc
     1261 ctttgtccga caagagaatt ggccatacag gcggcagaag agatcagaaa gctcgggaag
     1321 tatatgcacg gcgtcagggt gcttccgatc tacggcggac aggatatttc aaaacagatc
     1381 cggtcgctga aaggaaatgt gc
//
LOCUS       DAOSYE010000700         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84863_length_1400_cov_1.744981, whole genome shotgun sequence.
ACCESSION   DAOSYE010000700 DAOSYE010000000
VERSION     DAOSYE010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84863_length_1400_cov_1.744981"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLU43_10695"
     CDS             complement(<1..1154)
                     /locus_tag="PLU43_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28921.1"
                     /translation="MKNKTWIYGNFILIIVTFLLLFFLLSDYTNKKWKDTAVENVHLS
                     LSEGTYNLTMTYQAVSDTYLEAYITSNNQEYVIWGAILPAAKGAGSSSTFSFPLEYSV
                     LNNSLGFRCPYVQGEAVQFSEIQIKTAEFPLKQAASIVLYLFTAFLLCFFVKDFRMRF
                     YAEGIWTLSSIFLHPIFVRNLWFLLLYTIGYFIAFIIFFNKKNNTVNEDMAAFTFFYA
                     AALLFMEIFTTSSPLFSFNKGCDENIYYAIGHGLANGLHLYTEMFDHKGPFFFFVYMI
                     GYIISPGKYYGIYFIEVIFMTISLFCIYKIAAMFIKKEIAYMAAFSALPLLLNTNYMQ
                     TGGSFEQMAVPAMLGMLYFAFFYFSPALPSEISEKKQLVGFFLQGLLTAA"
     gene            complement(1144..>1400)
                     /locus_tag="PLU43_10700"
     CDS             complement(1144..>1400)
                     /locus_tag="PLU43_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO28922.1"
                     /translation="LGFVYAVWAVVNKFTNPAAPMGWTSTVIIQLVLGGMILFVLGLL
                     GEYVGRMYISMNNAPQYVIKTMIGYEKTEVSNGRDNYNEK"
BASE COUNT          494 a          302 c          223 g          381 t
ORIGIN      
        1 atcgcagcgg taagcagacc ctgtaaaaaa aatccgacca gctgtttttt ttcagaaatt
       61 tcagaaggca gggccggcga gaaataaaag aaagcaaaat acagcattcc cagcatggcc
      121 ggcacagcca tttgctcaaa tgagcctccc gtctgcatat aatttgtgtt cagcagcagt
      181 ggcaacgcac taaatgctgc catataggca atttcttttt tgataaacat tgcggcaatt
      241 ttataaatac agaaaagcga aatcgtcata aagatgacct caataaaata aataccgtaa
      301 tatttaccag gtgaaatgat atatccaatc atataaacaa aaaagaaaaa tggaccttta
      361 tggtcaaaca tttccgtata taaatgcagt ccgtttgcca gtccatgtcc gatcgcataa
      421 taaatattct cgtcgcagcc tttattaaat gaaaacaagg gcgaggaagt cgtaaagatc
      481 tccataaata ataatgccgc tgcataaaaa aatgtaaagg cagccatatc ttcattgacc
      541 gtattatttt ttttattaaa aaaaataata aatgctataa aataaccaat cgtatacagt
      601 aataaaaacc acaaatttct gacaaatatc gggtgcagaa atatactgct cagtgtccag
      661 attccttccg cataaaaccg cattctaaaa tctttcacaa aaaaacagag caaaaatgca
      721 gtgaacaaat agagcactat acttgctgcc tgcttcagcg gaaattccgc agtctttatc
      781 tgaatctctg aaaactgcac ggcctccccc tgcacatacg gacatctgaa acccagactg
      841 ttattcagta ctgaatattc caggggaaac gaaaaagtac tggaagaacc ggctcccttc
      901 gcagcaggaa gtatggctcc ccaaattaca tattcctgat tattggatgt tatatatgcc
      961 tctaaatagg tatcagatac tgcctgatag gtcattgtca gattatacgt cccctcggaa
     1021 agactcagat gtacattttc aacagccgta tccttccatt ttttattagt ataatcagac
     1081 aataaaaaaa ataacagcaa aaaagtaact atgatcaata taaaatttcc ataaatccat
     1141 gttttatttt tcattgtaat tatccctgcc gtttgatact tccgtttttt catatcctat
     1201 cattgtcttt attacatatt ggggtgcatt gttcatactg atatacatcc tgccaacata
     1261 ttcaccaagg agacccaata caaataagat catgccgccc aatacaagct gaatgatcac
     1321 tgttgatgtc catcccatcg gagccgccgg attcgtaaac ttattaacaa cagcccaaac
     1381 agcatataca aaacccagca
//
LOCUS       DAOSYE010000701         1399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_84886_length_1399_cov_3.926339, whole genome shotgun sequence.
ACCESSION   DAOSYE010000701 DAOSYE010000000
VERSION     DAOSYE010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84886_length_1399_cov_3.926339"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="cbiD"
                     /locus_tag="PLU43_10705"
     CDS             <1..1017
                     /gene="cbiD"
                     /locus_tag="PLU43_10705"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HOO28923.1"
                     /translation="VTNVTADTDETVFRAGQAGPAYTYVRCAVRKDSGDDPDITNKVL
                     VYASVRLCPDKQEICIDGGEGIGRVTRPGLNQQIGKAAINAVPRQMIEQAVEEICEEY
                     GYEAGIEVIVSIPGGELLAKKTFNPRLGIEGGLSILGTTGIVEPMSEAALLDTIELEM
                     KQKMSLGEQYLIMAPGNYGLDFINRTLHIDLEQCIKCSNYIGDSIDCACRLGAKGLLL
                     VGHIGKLVKLGAGVMNTHSRCADARMETLVSCAILAGADAQLARKLLSCVSTEEALAL
                     LVQAECLDQTMKILMERITFHLSHRADEQIITGAVVFSERYGILGYAGEAERLLEAAV
                     RRDV"
     gene            1018..>1399
                     /locus_tag="PLU43_10710"
     CDS             1018..>1399
                     /locus_tag="PLU43_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HOO28924.1"
                     /translation="MVHFVGAGPGAVDLITVRGKELIGHADIIIYAGSLVNRELLLFA
                     KKACRFYDSAYMTLAEVLGVMKQGEQEGKDIVRLHTGDPSLYSAIREQMDGLEKYHIA
                     YEVCPGVSSFCAAAASLQQEYTLPG"
BASE COUNT          362 a          268 c          430 g          339 t
ORIGIN      
        1 gttacaaatg tgacggcaga tacagatgag actgttttcc gtgcagggca ggcaggccct
       61 gcatatacgt acgtacgctg tgccgtaaga aaagacagcg gggatgatcc tgatattacg
      121 aataaagttt tagtttatgc atccgttcgt ctttgtccgg ataaacagga gatctgcata
      181 gacggcggag aagggatcgg cagagtgacg aggcctggtc tgaatcagca gatcggaaaa
      241 gccgccatta atgcggtccc aaggcagatg atagagcagg cagtggaaga aatatgtgag
      301 gaatacggat atgaggccgg cattgaagtc attgtttcga taccgggcgg agagctgctt
      361 gcaaaaaaga cgtttaatcc gcggcttggt attgaaggag ggctttcaat acttggtact
      421 accggtatcg tagagccgat gagcgaggcg gcgctgcttg acacgataga gctcgagatg
      481 aagcagaaaa tgtctttggg agaacagtat ctgatcatgg cgcctgggaa ttatgggctc
      541 gattttatca acaggaccct gcatatcgat ctggagcagt gtatcaaatg cagtaattat
      601 atcggagatt ccatagactg cgcctgcagg cttggagcaa agggactgct gcttgtcgga
      661 catatcggaa agcttgtaaa gcttggtgcc ggcgtgatga atacgcattc cagatgtgcg
      721 gatgcaagaa tggaaacgct tgtttcctgt gcgattttag caggggcaga tgcgcagctt
      781 gcacgaaagc ttctttcctg cgtttcgacc gaggaggcgc ttgcactgct tgtgcaggca
      841 gagtgtcttg accagacaat gaaaattcta atggagcgta tcacgttcca tttatctcat
      901 agagcagatg aacaaatcat aacaggtgca gtggtctttt ccgaacgtta cggtattctt
      961 gggtatgcgg gggaggcaga gagactgctg gaggctgcgg taaggaggga tgtataaatg
     1021 gtgcattttg tcggtgcagg tccgggagct gttgatctga tcacagtcag gggaaaggag
     1081 ctgatcgggc atgcggatat tattatttat gccggttcgc ttgtgaaccg ggagctgctt
     1141 ttgtttgcaa aaaaggcctg caggttttat gacagtgctt atatgacgct tgcagaagtc
     1201 cttggggtga tgaaacaggg agaacaggaa ggaaaagata tcgtccggct gcatacgggt
     1261 gacccgagtc tttatagtgc gatcagggaa cagatggacg gactggaaaa ataccatatt
     1321 gcgtatgagg tctgtccggg agtcagcagc ttttgtgcgg cagcggcatc gttgcagcag
     1381 gaatatacac ttcccggcg
//
LOCUS       DAOSYE010000702         1398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85000_length_1398_cov_3.590469, whole genome shotgun sequence.
ACCESSION   DAOSYE010000702 DAOSYE010000000
VERSION     DAOSYE010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85000_length_1398_cov_3.590469"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..160)
                     /locus_tag="PLU43_10715"
     tRNA            complement(86..160)
                     /locus_tag="PLU43_10715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Pro,seq:tgg)
     gene            complement(270..749)
                     /locus_tag="PLU43_10720"
     CDS             complement(270..749)
                     /locus_tag="PLU43_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO28925.1"
                     /translation="MKILIVSDTHRQNGKLWKVIDKVKPIDMLIHCGDVEGDDKEIEM
                     RAECPIHVVAGNNDIYYDLDDEQEFLLGRYKVLLVHGHRHHIYAGVASLFHLAKERGV
                     DIVMFGHLHVPMLEQKNGITVLNPGSLTYPRQPNRRPSYIIMELDEKGEAHYTLNYL"
     gene            complement(768..1364)
                     /gene="rdgB"
                     /locus_tag="PLU43_10725"
     CDS             complement(768..1364)
                     /gene="rdgB"
                     /locus_tag="PLU43_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOO28926.1"
                     /translation="MGKTIVFATGNEGKLKEIRMVLADLDVTVLSMKEAGVCTEIEEN
                     GTSFEENAVIKARAVMKETGMLTLADDSGLEIDYLNKEPGIYSARYMGEDTPYSIKNR
                     ELIKRLDGVPDEKRTARFVCAIAAVYPDGTEETVRAVMEGRIGYEEKGTHGFGYDPVF
                     YLPAYNCMSAELPLEEKNKISHRGKALRLMKEKLEKNN"
BASE COUNT          345 a          400 c          234 g          419 t
ORIGIN      
        1 gattcgaacc cccgacccac ggcttagaag gccgttgctc tatccaactg agctacgaac
       61 acataaattc aggaacttct acgcctcggg gtgacaggat tcgaacctgc gacctcctgg
      121 tcccaaacca ggcgctctag ccaagctgag ccacaccccg ctgtgccttc acaaacacac
      181 agcaacagtt atgcaaatcg tgacgcaagt catattatat aatagtaaaa aaggattgtc
      241 aactatctat tttccttatt ttcaaggaac tataaataat tcagcgtata atgcgcttct
      301 cccttttcat ccagttccat aataatatac gacggtcttc tgttcggctg cctcggatat
      361 gtcagactgc cgggattcaa taccgtaata ccgttcttct gttcaagcat cggtacatgc
      421 agatgaccga acatcacaat atcgacgccc cgctctttgg ccagatgaaa taacgatgca
      481 acacccgcat aaatatgatg tctgtgacca tggaccagca gcactttata tctgccaagc
      541 aaaaattcct gctcgtcgtc aaggtcataa taaatatcat tatttcccgc cactacatga
      601 atcggacatt ccgccctcat ctcgatttcc ttgtcgtccc cttccacatc tccgcaatgg
      661 atcagcatat cgatcggttt tactttatcg atcaccttcc atagctttcc gttttgtctg
      721 tgtgtgtcac ttacaatcaa tattttcatc tgttttcgcc agccaattca attatttttt
      781 tccagttttt ctttcatcag gcgcagtgcc tttccacgat gactgatttt atttttttct
      841 tctaacggca attcagccga catacagtta tatgccggaa ggtaaaaaac aggatcataa
      901 ccaaagccat gtgtaccttt ctcttcatat ccgatcctgc cttccatgac cgcccgcacc
      961 gtttcttccg ttccatccgg ataaacagct gcgatcgcgc acacaaaacg cgcagtcctt
     1021 ttctcgtccg gcactccgtc aagccgtttt atcaattctc tgtttttaat gctgtaaggc
     1081 gtatcctcgc ccatataacg ggctgaataa atacccggtt ccttattcag atagtcgatc
     1141 tcaagtccag aatcatcagc gagcgtcagc atcccggttt ctttcatgac tgccctcgct
     1201 tttatgactg cattttcttc aaaagaagta ccattctcct ctatctccgt gcagacacct
     1261 gcctctttca tagaaagcac cgtaacgtca agatccgcaa gcaccattcg tatttccttt
     1321 aattttcctt cgtttccggt cgcgaatacg atcgtttttc ccatgctctc atctccctgc
     1381 ctttcctatt gccgcatt
//
LOCUS       DAOSYE010000703         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85136_length_1397_cov_2.779434, whole genome shotgun sequence.
ACCESSION   DAOSYE010000703 DAOSYE010000000
VERSION     DAOSYE010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85136_length_1397_cov_2.779434"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1397
                     /locus_tag="PLU43_10730"
     CDS             13..>1397
                     /locus_tag="PLU43_10730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO28927.1"
                     /translation="MYTGKKIKYPLKYKMFTLVLVAMVPLLSVMIYLIFALLSYSNSY
                     DSIVSNMTIANNYNLNFKEEMDESIYKLAVGYVTFDMIDEDPTLQNPYTLLDELRGEF
                     SELMKITTDKESRGWLQSLLRNIDTLKDRIDDIRENVEQGGLYEENIEMLDNNIYILT
                     ELIQDDIQYYIYYQTESIDRLKTQLNKQVYSFIVSWTIMTAVIVLLVVVITSLILKSI
                     IKPVEELCSVTERIADGDFTSRAGVNTKDEMSVLAESVNDMSEHLQIMVDKIKEDERK
                     MRHAELRLLQEQINPHFLYNTLDTIVWLIECNDAESAVNIVVALSDFFRLVLSRGNEY
                     ITIREEEQHIRSYLEIQQVRYRDILEYEIDIDPDIYEYKILKLTLQPIVENSLYHGIK
                     YKRAKGLIKVTGERKQACIILTVQDTGVGMEPEELERLRREISVPSKESRTGFGLANV
                     SERIRMNFGAE"
BASE COUNT          438 a          245 c          351 g          363 t
ORIGIN      
        1 aaaataaata ttatgtatac aggaaaaaag ataaaatatc cgttaaaata taaaatgttc
       61 acacttgtac tggtagcaat ggtaccgctg ctgtcggtaa tgatctatct gatctttgct
      121 cttttaagtt acagtaattc ttatgattca attgtaagca atatgacaat agccaataat
      181 tataatctga atttcaaaga agagatggat gaaagtattt ataaactggc agttggatat
      241 gttacatttg atatgatcga tgaagatccc acattacaga atccatatac gctgctggat
      301 gagctccgcg gtgaattcag tgagctgatg aagatcacga cggacaagga gagcaggggc
      361 tggctgcaaa gtttgcttcg gaatatcgac acgctcaaag accggatcga cgatatcagg
      421 gaaaatgtcg aacagggtgg tctgtatgaa gaaaatatag agatgcttga taacaatatc
      481 tatattctta cagaactgat ccaggacgat atccagtatt atatctacta tcagacagag
      541 agcattgaca gactgaaaac acagctgaac aagcaggtat attcctttat tgtatcatgg
      601 acgatcatga cggccgtgat cgttctttta gtcgttgtta ttacctctct tattctgaaa
      661 agtattataa agcccgtaga ggaattgtgc agtgtgacgg aacgaatcgc ggacggagat
      721 tttacgagcc gggccggcgt gaatacgaag gatgaaatgt ctgtcctggc tgagagcgta
      781 aatgatatgt cggagcatct gcagatcatg gtagataaga tcaaggaaga tgagcgcaag
      841 atgcgccatg ccgagctgcg tcttttgcag gagcagatca atccgcattt tttgtacaac
      901 acactggata cgatcgtgtg gctgatcgag tgtaatgatg cggaaagcgc ggtcaatata
      961 gttgtggcat tgtcagactt tttccgcctt gtattaagca ggggaaatga atatattacg
     1021 atccgtgaag aggagcagca tattcgaagc tatctggaga tccagcaggt gcgttaccgg
     1081 gatattctgg aatatgagat cgatatcgat ccggatattt atgaatataa gatattgaaa
     1141 ctgaccttgc agcccattgt ggaaaattcc ctgtatcatg gaatcaaata caagagggcc
     1201 aagggactga tcaaggtaac aggagaacgt aaacaggcat gtatcattct gacagtgcag
     1261 gataccggtg tcgggatgga gccggaagaa cttgaacggc tgcgtaggga aatttccgta
     1321 ccgtccaagg agagcagaac cggcttcgga ctggcaaatg tcagtgaacg tatccgcatg
     1381 aatttcggag cggagta
//
LOCUS       DAOSYE010000704         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85190_length_1397_cov_1.362146, whole genome shotgun sequence.
ACCESSION   DAOSYE010000704 DAOSYE010000000
VERSION     DAOSYE010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85190_length_1397_cov_1.362146"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLU43_10735"
     CDS             complement(<1..940)
                     /locus_tag="PLU43_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28928.1"
                     /translation="MKTKKEAANQIQKNYRISESLKKIIRIERREPDRLELIKYLIHF
                     DINHFTGRYSDEWIERELAEYGKKIEFYHKLQPEAGKILHVMTKAYQMGGHTPIVSNW
                     IDYDDTKSYAVVFTQMKPVDIPCFFRETAEKNGAKLLFLRGRDDMEKAKELLKISDRY
                     EKIVLHIHMYDIVPILAYSNENWKRPVYFYNHANFLFSLGISVSDCVLCICRYDKIKA
                     IRYRGAKNACVLPLPLKTVKNHEVILQEKGADRAKFREEICQIYAIPADGRIILSMGA
                     DFKYEKIIDYDFAAFAAHLLQETENTYFFIIGADPSK"
     gene            complement(1120..>1397)
                     /locus_tag="PLU43_10740"
     CDS             complement(1120..>1397)
                     /locus_tag="PLU43_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOO28929.1"
                     /translation="QVGRLFVHSNPKSFEKYLKMQWEKSCNEQKEFLFVNAWNEWAEG
                     TYLEPDEKYGYGYLRAVRRVKEKLMGQSKDTAKGCCQSGRDSCNSNR"
BASE COUNT          395 a          323 c          189 g          490 t
ORIGIN      
        1 cttttgacgg atcagctccg ataataaaaa aatacgtatt ttccgtttcc tgcagcagat
       61 gtgcggcaaa tgccgcaaaa tcataatcta taattttttc atatttaaaa tcagctccca
      121 ttgaaagaat aattcttcca tctgccggaa tagcatatat ctggcagatc tcttcgcgaa
      181 acttcgctct gtctgctccc ttttcctgca atattacctc atgatttttc acagtcttca
      241 acggcagcgg caatacacag gcatttttcg cacctctgta cctgattgct tttattttat
      301 catatcggca gatacacagt acacaatcag aaacagaaat tccaagcgaa aataaaaaat
      361 tggcatgatt ataaaaatag accggtcttt tccaattttc attagaatac gccaaaattg
      421 gaacaatatc atacatatga atatgcaata caattttttc gtatctatcc gatattttca
      481 ataattcctt tgccttttcc atatcatccc tgccgcgcag aaataaaagc tttgctccgt
      541 ttttttctgc cgtctcccga aaaaaacatg gtatatcgac tggtttcatt tgtgtaaaaa
      601 caactgcata actttttgta tcatcataat caatccagtt acttacgatc ggtgtatggc
      661 ctcccatttg ataagccttc gtcattacat gaagaatttt cccagcttcc ggctgtaatt
      721 tatggtaaaa ttcgattttt ttcccatatt ccgccagttc cctctcgatc cattcatcac
      781 tgtacctgcc cgtaaaatgg ttgatatcaa aatgtatcag atactttatc aactcaagac
      841 gatccggttc tcttctttca atccgaatta tctttttaag cgattcagat attctataat
      901 ttttctgaat ctggtttgcc gcttcttttt ttgttttcat ctgttatact ccatatcctg
      961 cacaattaat ctccgatgac ttccggatat tttttcccag gcatataata gatatgtcca
     1021 tttaatttct gataattttt ttcatttatg tgctttagca tgtctttaaa atatacactt
     1081 cccaaaatga ccttgaccaa aaagtaacat ttcagggctt catctatttg aattacagga
     1141 atcccgtccg gattggcaac aacccttcgc cgtatctttg ctctgaccca tcagcttttc
     1201 cttaactcgc ctaacagctc gcaggtaacc ataaccgtat ttctcatccg gctccaagta
     1261 agtcccttct gcccattcat tccacgcatt aacaaaaaga aattcttttt gttcattaca
     1321 gcttttttcc cattgcattt ttaaatattt ttcaaaggac tttggattac tatgaacaaa
     1381 aagacgtcct acttgat
//
LOCUS       DAOSYE010000705         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85424_length_1394_cov_2.707991, whole genome shotgun sequence.
ACCESSION   DAOSYE010000705 DAOSYE010000000
VERSION     DAOSYE010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85424_length_1394_cov_2.707991"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLU43_10745"
     CDS             <1..931
                     /locus_tag="PLU43_10745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO28930.1"
                     /translation="VKQRAGRFFQSEGYETVHLLTIILTPDVQEAKKFVLDDSFCWIV
                     DSSVNRLIIFENQREDFYGLRKFVEQACTLTESGGTEQQERVAEEKPKQRKKTPWYIK
                     PAYQRTGIFAKNYAPVNFALVAVNIAVFIYLSIIGSTKDLEFMLKYGVMYVPYVTEYH
                     EYYRFFTCMFLHFGIQHLAGNMVVLFFLGDNVERAAGKIKYLIIYIGSGLAGSFGSFL
                     FSYLFNQNIVSAGASGAIFGVIGALLWIVIRNRGRLEDITTFRVALLIFYALYMGIRG
                     ENIDNAAHVAGLLGGFLLAVILYEKKSNSQERA"
     gene            958..>1394
                     /locus_tag="PLU43_10750"
     CDS             958..>1394
                     /locus_tag="PLU43_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP-2 sterol transfer family protein"
                     /protein_id="HOO28931.1"
                     /translation="MKINIYYGGRGILDDPTLYVINKIQDVLMELNVKVERFNLYELK
                     NTITTLPATINDADGIILATTVEWYGIGGYMMQFLDACWLYGNKEKISRTYMSPIVMS
                     TTSGEREAKTSLAVSWEILGGLPCSGLCGYVNDMMSFELNKDYL"
BASE COUNT          393 a          223 c          368 g          410 t
ORIGIN      
        1 ggtaaaacag agagcaggca ggttttttca aagcgaagga tacgaaacgg ttcatctgct
       61 gacgatcatt ttgacaccgg acgtgcagga agcgaaaaag tttgtattgg atgacagctt
      121 ttgctggatc gtggattctt ctgtgaacag gcttatcata tttgaaaatc agagagaaga
      181 tttttatgga ctgcgtaagt ttgtggaaca ggcatgtacc ttgacagaaa gcggaggcac
      241 agagcagcag gagcgggttg cggaggagaa gccaaaacag cgaaaaaaaa cgccgtggta
      301 tataaaaccg gcttatcaga ggaccggcat ttttgcaaaa aattatgccc ctgtaaattt
      361 tgcacttgtt gctgtaaata ttgcggtttt tatttattta tcgatcattg gttcaacaaa
      421 agatctggaa ttcatgttaa aatacggagt gatgtatgta ccatatgtta cggagtatca
      481 tgaatactat cgttttttta catgtatgtt cctgcatttt ggaatacagc atcttgccgg
      541 aaatatggtc gtattatttt ttcttggaga taacgtagaa cgtgcggcag gaaaaataaa
      601 atatctgatt atttatattg gaagcggctt agcgggcagc tttggttctt ttcttttttc
      661 ttatttgttc aaccagaata tcgtgtcggc cggtgcatcg ggggctattt ttggtgtgat
      721 cggtgcattg ctttggatcg ttatccgtaa caggggacgt ttggaagata taacaacgtt
      781 tcgtgtggca ttgctgattt tttatgcatt gtatatgggc atcagaggag aaaatattga
      841 caatgctgcg catgttgccg ggctgcttgg aggatttctt ctggcggtca ttctgtatga
      901 gaagaagagc aatagtcagg aacgggcatg atgtttgacg gaaagggaag cgagtaaatg
      961 aaaattaata tttattatgg cggaagaggg atattggatg accctacttt atatgtcatc
     1021 aataaaatcc aggatgtatt gatggaactg aacgtcaagg tggaacgttt caatctgtat
     1081 gagctgaaaa atacgattac gacacttccc gcgacgatca atgatgccga tggaatcata
     1141 cttgctacga ccgtggaatg gtatgggatc ggcggatata tgatgcagtt tctggatgcc
     1201 tgctggctgt atgggaataa agaaaaaatt tccaggacat atatgagtcc gatcgtgatg
     1261 tccacaacgt ccggtgaacg ggaagccaag acaagccttg ccgtttcatg ggaaattctc
     1321 ggggggctgc cctgcagcgg actgtgcggc tatgtgaatg atatgatgtc gtttgaattg
     1381 aataaggatt atct
//
LOCUS       DAOSYE010000706         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85603_length_1392_cov_3.056844, whole genome shotgun sequence.
ACCESSION   DAOSYE010000706 DAOSYE010000000
VERSION     DAOSYE010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85603_length_1392_cov_3.056844"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1392
                     /locus_tag="PLU43_10755"
     CDS             32..>1392
                     /locus_tag="PLU43_10755"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO28932.1"
                     /translation="MIKKELQDELQDIAYLHPEILGIAIVTQKKECVMYDSITNSSMN
                     SYCFPERDENWLNVAEETFHNTQTVYSEVIRKEQEDKSEQDVIYLAHRIVDINNYRQG
                     TLGSIMICLDEKDIRETYAQESENAANVSFLCDAYGNVISSTNAALLGVNIMTEDTEE
                     KEAFPYMQTAYEEQIREAVGRYQIMDTKFFSVYTKPVLNDQFVQVSIRDNRELLKDFK
                     YIFEIILLLTILIIITSCLIMMKFATSMQYRVGRITSAMDKAYKGDYSVQIDTEWQDE
                     FGKIARHFNHMVRKIDQSGKMEKEALLREKNAELKALEAQINPHFLYNTLDAINWMAI
                     ENEQFKISKMLKSLAMILRYSIHKSNSVVSIESEMDYLKKYIYLQQQRFSFSFQCILN
                     VEEEVMQLKMHKLLFQPLIENAIIHGFPGKTGNDTITISIRKKDEKHLVIQVKDNGKG
                     MDQ"
BASE COUNT          489 a          214 c          330 g          359 t
ORIGIN      
        1 attaaatgta tgggattcca gggattacta tatgataaaa aaagaactgc aggatgagct
       61 gcaggacatt gcgtatcttc atccggagat actgggaatc gcaatcgtca cgcagaagaa
      121 agaatgcgtg atgtatgatt ccataacaaa cagctccatg aacagttact gttttcctga
      181 aagagatgaa aactggctga atgtggcgga agaaactttt cataatacac agacggtgta
      241 ctcggaagtg atcaggaagg aacaggaaga taaaagtgaa caggatgtta tttatcttgc
      301 ccatcggatc gtggatatca ataattacag gcagggtacg ctgggaagca ttatgatctg
      361 tttagatgaa aaggatatca gggaaacata tgcacaggaa agtgagaacg cggcaaatgt
      421 ttcctttttg tgtgatgcat atggaaatgt tatttcaagc acaaacgcgg ccctgctcgg
      481 tgtcaatatt atgacagagg atacggaaga aaaagaggcg tttccataca tgcagacagc
      541 gtatgaagag cagatcagag aagcggtcgg ccggtatcag atcatggata caaaattttt
      601 cagtgtttat acgaaaccgg tattaaatga tcagtttgta caggtcagca tcagagataa
      661 cagagaactg ctgaaggatt ttaaatatat ttttgaaatc attcttttgc ttacgatcct
      721 gattattata acaagctgtc tgatcatgat gaaatttgca acctccatgc agtacagggt
      781 aggaagaata acgtcggcga tggataaggc ttataaaggc gattattctg ttcagatcga
      841 tactgaatgg caggatgagt tcgggaaaat agcaagacat tttaaccata tggtacgaaa
      901 gatcgatcag tcggggaaaa tggaaaaaga agcgctgctc agggaaaaaa atgcggaact
      961 gaaagcgctt gaagcgcaga taaatcctca ttttctgtat aatacactgg atgcaatcaa
     1021 ctggatggca atagaaaacg aacagttcaa aatcagtaag atgctgaaaa gtcttgcgat
     1081 gattctgcgc tacagtattc ataaaagcaa cagtgtcgtg agcattgaaa gtgagatgga
     1141 ttatttaaaa aaatatattt atcttcagca gcagagattt tctttttctt ttcaatgtat
     1201 tttaaatgta gaagaggaag tcatgcagct gaaaatgcat aaattacttt ttcagccttt
     1261 gatagaaaat gcgatcattc atggatttcc aggaaaaaca ggaaatgata cgatcacgat
     1321 ctcgatcagg aaaaaggatg agaagcattt ggtcatacag gtcaaagaca atggaaaagg
     1381 aatggatcag ga
//
LOCUS       DAOSYE010000707         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85706_length_1391_cov_3.457335, whole genome shotgun sequence.
ACCESSION   DAOSYE010000707 DAOSYE010000000
VERSION     DAOSYE010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85706_length_1391_cov_3.457335"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLU43_10760"
     CDS             complement(<1..668)
                     /locus_tag="PLU43_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28933.1"
                     /translation="MKKMIAAMLCGTLLMTMLTGCGSTETVSDTSEQAAEDTGTNDAD
                     EGTSEEVQSDTESSDAAQAAIDERKAEAEESGEYEKIVISFFDWTGAPAGIDRINEAI
                     SAHTEETLGLDVELMIIDSAAYSDDIKLMLSSGEQVDIFSTCGPGYTTCVNNGYVLDL
                     EEDDMMAKYGQGISEKVREEYLDACRIGDVLYGIPPIKDYAIQTADVCIGTEYLDAIG
                     GDYYG"
     gene            complement(934..>1391)
                     /locus_tag="PLU43_10765"
     CDS             complement(934..>1391)
                     /locus_tag="PLU43_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO28934.1"
                     /translation="RIGVAAGERDNFHTIMRMNGYQKALFEAGILYDPGIVEYVRWER
                     EAGYKVAPTMISNHVSAVFCMSDTIAGGIYDYMREKGMTMSVAGYDNRAMADYMAPGL
                     TTMALPLNEIGRQSAEKLIGILQGNAIEEHFEQRIPCTLIERESVTAIE"
BASE COUNT          345 a          384 c          227 g          435 t
ORIGIN      
        1 ccataataat ctccgccgat ggcatccaga tattctgttc caatacaaac atccgccgtc
       61 tggatcgcat aatccttaat tggaggaatt ccgtaaagca catctccgat cctgcaggca
      121 tccagatatt cttcccgtac cttttcgctg atgccctgtc cgtattttgc catcatatca
      181 tcttcttcca gatcaaggac atatccatta ttgacacagg ttgtataacc cggaccgcat
      241 gtggaaaaaa tatctacctg ttctccggat gaaagcatca gtttgatatc atcactgtat
      301 gctgccgaat caatgatcat caattcaaca tccagtccaa gtgtttcctc cgtatgagca
      361 ctgatcgctt cattgattct gtcgatacct gccggcgcac ccgtccagtc aaaaaacgaa
      421 ataacaattt tttcatactc cccgctctct tcggcttctg cctttctttc atcgatcgcc
      481 gcctgtgccg catcacttga ttcggtatca gactgcacct cctccgaagt tccctcatcc
      541 gcatcatttg ttccggtatc ctccgctgcc tgttccgatg tatccgaaac cgtttccgta
      601 cttccgcatc ccgtcagcat tgtcatgagc agtgtgccac ataacattgc cgcaatcatc
      661 tttttcattt tccttcctcc ttgttttgcg ttaaatagtt aaatggttaa tcgtttaact
      721 ttatgttaat attttatatc tgtcaatttt atttgtcaat atgaattaaa acgacagatt
      781 gtacaataat atttatttat ttttgtttat attcactaat tcaaacgttt tcgtatatta
      841 aacgattaac tctattcgtt taaaaaaacg cctgccttgc agacgttttt tgtttaagaa
      901 aatgtataac aacagataac agcaggaccg gccttattct atcgccgtca cagattcccg
      961 ttcgatcaat gtacacggga tccgctgttc aaaatgctcc tcgatcgcat tcccctgtaa
     1021 tattccgatc agtttttcgg ccgactgacg cccaatctca ttgagcggaa gcgccatagt
     1081 cgtcaggccc ggcgccatat aatcggccat cgcccgatta tcgtatccgg ctacggacat
     1141 cgtcatcccc ttttcccgca tataatcgta aatacctcct gcaatcgtat ccgacataca
     1201 gaagaccgca cttacatgat tggatatcat tgtaggtgcc accttgtatc cagcctctct
     1261 ctcccacctt acatactcaa cgatgcccgg gtcatataag attccggctt caaaaagagc
     1321 cttctgatag ccgttcatac gcatgatcgt atgaaaatta tctctctctc cggctgccac
     1381 tcctatcctc t
//
LOCUS       DAOSYE010000708         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85729_length_1391_cov_2.748503, whole genome shotgun sequence.
ACCESSION   DAOSYE010000708 DAOSYE010000000
VERSION     DAOSYE010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85729_length_1391_cov_2.748503"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PLU43_10770"
     CDS             <1..1126
                     /locus_tag="PLU43_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOO28935.1"
                     /translation="CMVTCGKLFSTLFGAPYLWMMIVGAAFVLIYTILGGFLAESASD
                     FMQSIVMITALAMVLIIGTADAGGVHAVISNAKSFPGFLKFFGIASPVMENGVQAVAD
                     GTPVFGEAGVYGFLTVVSTMAWGLGYFGMPQVLLRFMAIRSEKELPRARHIAMIWVII
                     SLAAAIFIGIVGRTLFPTALKTASEAENVFILLSTNLLPALLAGFVMAGILAATISSS
                     DSYLLIAASAFSKNIYQGLFRKKASDKEIMAVSRGMLLLITIIAIIIASDENSVIFTI
                     VSFAWAGFGATFGPLMLFSLFWKRMNRQGAIAGMIGGAGMVFLWKLAVRPLGGAFNMY
                     ELLPAFIFSGLCIVIVSLITAPPSEKLQKEFEEVRAGINC"
     gene            complement(1201..>1391)
                     /locus_tag="PLU43_10775"
     CDS             complement(1201..>1391)
                     /locus_tag="PLU43_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOO28936.1"
                     /translation="WAIFPGSGTGISIQELEKRIITEWLPTSGYEYADGPDIEVYFEA
                     DPENTTYEVWIPVIKKSI"
BASE COUNT          314 a          270 c          360 g          447 t
ORIGIN      
        1 ctgtatggta acatgcggga aattattttc taccttattc ggggcaccgt atttatggat
       61 gatgatcgtc ggtgcggcat ttgtattgat ttatacgata ttgggaggat tccttgcgga
      121 aagcgcttct gatttcatgc agtcaattgt gatgatcact gcacttgcca tggttttgat
      181 cattggtacg gcagatgcgg gcggcgttca tgcagtgatt tccaatgcga aaagctttcc
      241 gggattttta aagttttttg ggatcgcttc tccggttatg gagaatggcg tacaggctgt
      301 agcggatggg actccggtgt ttggagaagc aggtgtctat ggattcctta cggtcgtttc
      361 tacgatggca tgggggcttg gctattttgg aatgccgcag gtacttcttc gatttatggc
      421 gatccgcagc gagaaagaac ttccgagagc ccgtcatatt gcaatgatat gggtcataat
      481 ttctttagcg gctgcgattt ttatcggaat cgtcggcaga acactttttc cgacagcctt
      541 gaaaactgct tcggaagcgg aaaatgtttt tattttatta tcgacgaatc tgctgccggc
      601 attgcttgct ggttttgtta tggcaggtat tctggctgct acgatcagtt cctcggattc
      661 ttatcttttg atcgcagcat ctgctttttc aaagaatatt tatcagggtc ttttcagaaa
      721 aaaagcgtca gacaaagaga tcatggctgt ttccaggggg atgctgcttt tgattacgat
      781 catagcaatt atcattgcgt ctgatgagaa cagtgttatt tttacgatcg tatcatttgc
      841 ctgggcgggc tttggcgcga catttggtcc gctgatgctt ttttcactgt tttggaaaag
      901 aatgaaccgt cagggggcga ttgccgggat gatcggcgga gccggtatgg tatttctctg
      961 gaagcttgct gtccggccgt taggcggagc gtttaatatg tatgagctgc ttccggcctt
     1021 tatcttttcc ggtctttgta ttgtgatcgt ttcgcttata actgcgccgc cgtctgagaa
     1081 attgcaaaaa gagttcgaag aggtcagggc aggcattaat tgttaattgc cggacaggct
     1141 tatttaagat gtctgataaa aagggctggt ttatcaaaaa tgataaagca gcccttttcc
     1201 ttagatactt tttttgatta ccgggatcca gacctcatag gttgtatttt ccggatctgc
     1261 ttcaaaatac acttcaatat caggaccatc tgcatattca taaccggaag ttggaagcca
     1321 ttcggtaatg atacgctttt ccagctcctg gatcgaaata ccggttccgc tgccgggaaa
     1381 aatagcccag g
//
LOCUS       DAOSYE010000709         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85844_length_1390_cov_2.911610, whole genome shotgun sequence.
ACCESSION   DAOSYE010000709 DAOSYE010000000
VERSION     DAOSYE010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85844_length_1390_cov_2.911610"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLU43_10780"
     CDS             <1..284
                     /locus_tag="PLU43_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOO28937.1"
                     /translation="TKVDPWFLHQMKDILNAAENKLNKKQQETLFPLLFALTQTNMKR
                     IEEDISWFVDKFDYRNKDADWKNSKDAIPRTMQKLAGSYPSDGPYRDGC"
     gene            complement(266..892)
                     /locus_tag="PLU43_10785"
     CDS             complement(266..892)
                     /locus_tag="PLU43_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO28938.1"
                     /translation="MNAKFFDVKKEKQDAIINAALKVFAENGYRKASTDVIVKQAGIS
                     KGLLFHYFISKQGLYEFIYDYSVRYMKLELTQSVKKEEKDFFEVLKQIEQAKTRVMKN
                     YSYMQQFLNNVKFETHPDALAAIGDSKDVMTVTYNSIYKQADMTRFHDHVDVSRVIQM
                     ITWMSEGFIRDRFREPEPDLDEMSTEFGKYLAMLKAHFYREDNQQPSR"
     gene            complement(1001..>1390)
                     /locus_tag="PLU43_10790"
     CDS             complement(1001..>1390)
                     /locus_tag="PLU43_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO28939.1"
                     /translation="EVTESLAINDMERMKQILGNIKRLGVRIALDDFGTGYSSLSHIR
                     MIPLDVIKVDQSFVTELAVDRYAQAFVKMVGELASALGVKICVEGIETAEQFKVLENM
                     NVRMVQGFYFDRPMPKEQFEEKYVYNR"
BASE COUNT          384 a          366 c          245 g          395 t
ORIGIN      
        1 tgaccaaagt cgatccctgg tttttgcacc agatgaaaga catcctgaac gccgcggaaa
       61 acaaactgaa taaaaaacag caggaaacac ttttcccgct gctctttgca ctcacgcaga
      121 ctaatatgaa acgaatcgaa gaggacatct cctggttcgt ggataaattc gattaccgca
      181 acaaagacgc cgactggaaa aactccaagg atgccatccc gcgcacgatg cagaagcttg
      241 ccggcagcta cccgtcagat ggtccctatc gggatggctg ctgattgtct tcccggtaaa
      301 aatgtgcctt cagcatggca agatatttgc caaactccgt gctcatctca tccagatcag
      361 gttccggctc acggaatctg tcccggataa agccttccga catccatgtg atcatctgta
      421 tgacccggct gacatcgaca tgatcatgaa accgtgtcat gtcggcctgt ttataaatag
      481 aattataagt cactgtcata acatcctttg aatcgccgat cgcagccagt gcatccggat
      541 gggtctcgaa cttcacattg tttaaaaact gctgcatata ggaataattt ttcatgactc
      601 ttgttttggc ctgttcgatc tgcttcaata cttcaaaaaa atctttttct tcctttttga
      661 ccgattgggt cagttccagt ttcatatatc tgacactata atcatagata aattcataaa
      721 gtccctgctt gctgataaaa taatggaaca aaaggccctt ggatatcccg gcctgcttta
      781 cgatcacatc cgtacttgct tttctatagc cattctccgc aaacaccttc aatgccgcgt
      841 ttataatagc gtcctgtttt tctttcttta cgtcaaaaaa tttagcattc atatgtttta
      901 tgctcctccc acttatatca tcacattttc tccccaaaaa atgcttgacc tttccagtca
      961 agcataatat aacatacatt atgtaaattc gcaacaaaaa ctatctgtta tagacgtact
     1021 tttcttcaaa ttgttccttg ggcatcggcc tgtcaaaata aaaaccctgt accattctga
     1081 cgttcatatt ttcaagcacc ttaaactgtt cggctgtttc gatcccttcc acacagatct
     1141 taacaccaag cgcactcgcc agctctccga ccatttttac aaaggcctgt gcataccggt
     1201 ctaccgccag ctccgtcaca aagctctggt cgaccttgat cacatcaagc ggtatcatgc
     1261 ggatatgact taaggaagaa taacccgtac caaagtcatc aagcgcgatt ctgacaccaa
     1321 gccgcttgat attgccaagg atctgcttca tacgctccat gtcattgatc gcaaggcttt
     1381 ccgtaacctc
//
LOCUS       DAOSYE010000710         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_85857_length_1390_cov_2.576030, whole genome shotgun sequence.
ACCESSION   DAOSYE010000710 DAOSYE010000000
VERSION     DAOSYE010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85857_length_1390_cov_2.576030"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLU43_10795"
     CDS             <1..255
                     /locus_tag="PLU43_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO28940.1"
                     /translation="TAKDEISVIITKSPCVLLNKAPITKKCVVDSELCKKCGMCLKPG
                     CPAITKQPDGTAAIDDTMCNGCGLCEDLCKFGAISYAAVK"
     gene            267..836
                     /locus_tag="PLU43_10800"
     CDS             267..836
                     /locus_tag="PLU43_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOO28941.1"
                     /translation="METKNIMIVGVGGQGTLLTSRILGGIMLHAGYEVKLSEVHGMAQ
                     RGGSVVTFVRYGDKVAEPIVEEGQADVLIAFERLEAMRYAHFLKKDGVLIVNNQRIDP
                     MPVVMGAAQYPENIIEDLSANGKVISVDAMEEAKKLGNSKVFNVIVLGLAAKHMDFKK
                     EDWITVIEKTVKPKTIEINKKAFEVGYAI"
     gene            852..>1390
                     /locus_tag="PLU43_10805"
     CDS             852..>1390
                     /locus_tag="PLU43_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOO28942.1"
                     /translation="MIWNETKECMSRDEMSHLQSMRLVKLVDRVYHNVEFYRKKMQEV
                     GLEPGDISSIDDLNKLPYTTKDDLRDNYPFGLFAVPMSEIVRVHASSGTTGKATVVGY
                     TRRDIEIWSECVARAFSQAGISKADIVQIAYGYGLFTGGLGAHYGAEHLGATVVPMST
                     GNTKKLITMMEDFGATAIA"
BASE COUNT          396 a          245 c          410 g          339 t
ORIGIN      
        1 actgcaaagg atgagatttc cgttattatt acgaaatccc catgtgtgct tttaaataaa
       61 gctccgatca caaagaagtg tgttgtagac agtgaattgt gtaaaaaatg cggaatgtgt
      121 ctgaaaccgg gatgtccggc aatcacgaaa cagcctgacg gtacggcagc gatcgatgat
      181 acgatgtgca atggctgcgg cctttgtgag gatctgtgta agtttggtgc aatttcgtat
      241 gcagccgtga agtaggagga agagcaatgg aaacaaaaaa tattatgatc gttggtgtcg
      301 gcggacaggg aacgcttctc acaagccgta ttctgggagg catcatgctt cacgcagggt
      361 atgaagtaaa gctttcggag gtgcatggca tggcacagag aggcggttct gtcgtgacgt
      421 ttgtcagata tggggataaa gtggcggagc ctatcgtaga agaggggcag gcagacgtat
      481 tgattgcatt tgagcgtctg gaagcaatgc gttatgcgca ttttcttaaa aaggacggtg
      541 tgctgatcgt aaataatcag cggatcgatc caatgcctgt tgtgatgggg gcagcacagt
      601 atccggaaaa tatcatcgaa gatctgtctg caaatggaaa ggtgatttct gtggatgcca
      661 tggaagaagc aaagaagctt ggcaattcca aagtatttaa tgtgatcgta cttggacttg
      721 ccgcaaagca catggatttc aaaaaggaag actggatcac tgtaatcgaa aagacggtca
      781 agccgaaaac gatcgaaatt aataagaagg catttgaggt tggttacgct atttaacaca
      841 ggagggttct gatgatctgg aatgagacaa aagagtgcat gagcagggat gaaatgagtc
      901 atctgcaaag catgcgattg gtaaaactgg tagaccgcgt ttatcacaat gtggaattct
      961 atcgtaagaa gatgcaggag gtcggcctgg agccgggtga tatcagcagt attgacgatc
     1021 tgaacaagct gccgtataca acaaaggatg atctgcgtga taattatccg tttggtctgt
     1081 ttgcggtgcc gatgtcggaa atcgtcaggg tacatgcatc ctccggtaca acaggcaagg
     1141 caacagtcgt ggggtatacg agaagggata ttgaaatctg gtctgagtgt gtggcgcgtg
     1201 cgttctcgca ggcaggcatt tcgaaagcag atattgttca gatcgcgtat ggatatggtc
     1261 tgtttacggg aggccttggc gcacattacg gcgcggagca tttaggagca acagttgtgc
     1321 cgatgtctac cggcaatacg aagaaactga tcacaatgat ggaggatttc ggagcgacgg
     1381 caattgcctg
//
LOCUS       DAOSYE010000711         1388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86056_length_1388_cov_2.570893, whole genome shotgun sequence.
ACCESSION   DAOSYE010000711 DAOSYE010000000
VERSION     DAOSYE010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86056_length_1388_cov_2.570893"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLU43_10810"
     CDS             complement(<1..803)
                     /locus_tag="PLU43_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOO28943.1"
                     /translation="MNIKKWLKKITAAAVMTVLFAGMVHSTAKADEVVNFILNYDTTV
                     YTRQVYSPEIILINDGTESDAIKWITEVCNQVNFITNGEVTIDSAEVKAEVKKEIAAG
                     NQSIKLDLGDYTAQAIAEKKAAAAQALLLSAQSSSDTGMTPEIAANLNALGIDTKISE
                     CSTKYTVGQDRSLNIAVAAGRVNGTVLQPGQSFSYDTTILPRTTANGYGPGDIISNGT
                     HIKAIGGGICQVSSTLNNAVLKAGIVPTERHNHSQRVYYLKSGLDATVSS"
     gene            complement(928..>1388)
                     /locus_tag="PLU43_10815"
     CDS             complement(928..>1388)
                     /locus_tag="PLU43_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOO28944.1"
                     /translation="YFDRILVRANTRFMVMSGGQYELKRRRGTDNNRSQSGLELDVID
                     HYNGTQRSVKTLSGGESFKASLSLALGLSDEIQSSSGGVMVDTMFVDEGFGSLDEESL
                     QQAMRALYGLAEGNRLVGIISHVPELKEKIDRQIVVTKEKSGGSRAEIVC"
BASE COUNT          322 a          381 c          268 g          417 t
ORIGIN      
        1 gaggaaaccg ttgcatccag cccgcttttt aaataataaa cccgctggct gtgattatgc
       61 cgttctgtcg gaacaatgcc cgctttcagc accgcattgt tcaatgtgga ggaaacctgg
      121 cagatccctc ctcctattgc cttgatatgt gtcccgttgc tgataatatc accgggaccg
      181 tagccattgg ctgtcgtacg cggcagaatc gtcgtatcat aagaaaatga ctgtcccggc
      241 tgcaatactg ttccgttgac ccttcctgcg gcaacagcaa tattcagcga gcggtcctgc
      301 ccgaccgtat attttgtact gcattcagat atttttgtat caataccaag tgcattcaga
      361 ttggccgcaa tttctggtgt catacccgta tcggacgaag actgggcaga taacagcagc
      421 gcctgagcgg ctgccgcttt cttttccgca attgcctgtg ccgtataatc accaagatcc
      481 agtttgatac tttggtttcc tgccgcgatc tcttttttca cctcggcttt cacctccgca
      541 ctgtctatgg tcacttcccc gtttgtaatg aaattgacct gattacagac ttccgtgatc
      601 cactttatcg catcactttc cgttccgtca ttgatcagta tgatctcagg cgaataaacc
      661 tggcgtgtat agactgtcgt atcatagttt aaaataaaat tcacaacttc atcggctttt
      721 gccgtactat ggaccattcc cgcaaataat acggtcatga ccgccgccgc cgtaattttt
      781 ttcagccact tttttatatt catgctcttt ctccctttat cagctttcct tttgccacaa
      841 cacaagcatg cgtaaatcat atcatatttc gtttgcctat ttcaagctta atagtttctt
      901 ttgcatatat gtacacacca ccgatcctca gcaaacgatc tctgcccggc tgccgccgga
      961 tttttctttt gttacgacaa tctgtctgtc gatcttctcc ttaagttccg gcacgtgtga
     1021 aataattcct acaagccggt ttccctctgc taatccgtaa agtgctcgca ttgcctgctg
     1081 cagtgattcc tcatcaaggg agccaaagcc ctcatctacg aacatggtat caaccattac
     1141 acctcctgat gaagactgta tttcatctga aagtcctaaa gccaaagaaa gggatgcctt
     1201 aaaggattca cctcctgaaa gtgttttgac gcttcgctgt gttccattgt aatgatcaat
     1261 cacatccagt tccaggccgc tctggctgcg attgttatcc gtccccctgc gccgcttaag
     1321 ctcgtattgc ccgccggaca taaccataaa ccgcgtattt gcacgaacca gaatccgatc
     1381 aaagtatg
//
LOCUS       DAOSYE010000712         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86114_length_1387_cov_3.815315, whole genome shotgun sequence.
ACCESSION   DAOSYE010000712 DAOSYE010000000
VERSION     DAOSYE010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86114_length_1387_cov_3.815315"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLU43_10820"
     CDS             <1..290
                     /locus_tag="PLU43_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28945.1"
                     /translation="QAVEMTAPMGRCVMYSSQHPDKPFEISPNWLHNSETIVTGAVSP
                     SVRSFNRSVNLLSKGMLDPEKLVSGIYPYEEAQTAFEAAVRPDTFRIIITF"
     gene            305..568
                     /locus_tag="PLU43_10825"
     CDS             305..568
                     /locus_tag="PLU43_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO28946.1"
                     /translation="MIKKKVIVTAKAGLHARPANLLIKEAQHFQSEIEIGVNNQLYNA
                     KSLLGILAAGVDCGTEIEVVCNGSDEKAACDAVIKLLASGLGE"
     gene            561..>1387
                     /locus_tag="PLU43_10830"
     CDS             561..>1387
                     /locus_tag="PLU43_10830"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOO28947.1"
                     /translation="MNRSKEAKGVGKMRGYGVSKGLCYARILVLNEEPIVFPTKTGCI
                     LEEEEKFKDAHRSVLAETRALRDRTREESGNNAAEIIDIQCSLLKDNGLIDPIRKEIA
                     GGTGAAAAVENVMNGFIKQFEGMENQYFAQRAVDLKDLKNNLIYKILNIPRVDITMLA
                     EPTIIAGNDITPSQTARMDQHMVSGLLMEFGGNTSHTAILARTLDIPAIVGIKGLLKE
                     IKTGDMVGFDGETGEIFTNLTKVETASLQTKIQEEKQRKERLQVLAAGRAATLDGKLL
                     "
BASE COUNT          452 a          251 c          373 g          311 t
ORIGIN      
        1 aacaggcagt tgaaatgacg gcaccgatgg gacgctgtgt gatgtacagc tcacagcatc
       61 cggataaacc gtttgaaatc agccctaact ggctccataa ctcagaaacg atcgtaaccg
      121 gagcggtcag cccaagtgtt cgttccttta acaggtcggt caacctgctt tccaaaggaa
      181 tgctggaccc ggaaaagctt gtttccggaa tttatccata tgaagaggca cagacagcgt
      241 ttgaggcagc agtacgaccg gatacatttc gaattattat aacattttaa cggaggagct
      301 tgtaatgata aagaaaaaag tgatagtgac agcaaaagca ggcttacatg cccgtcctgc
      361 aaatttattg atcaaagaag cgcagcattt tcaatctgag atcgagatcg gtgtaaataa
      421 tcagctgtat aatgccaaat ctcttttggg aatattggca gcaggcgttg actgcggaac
      481 ggaaattgaa gtggtctgca acggaagtga tgaaaaagcg gcctgtgatg cagtgataaa
      541 actgcttgcc agcggtttgg gtgaatagaa gcaaagaagc aaagggggtc ggtaaaatgc
      601 gtggatatgg ggtttcaaaa ggcttatgtt atgccagaat attggtttta aatgaggagc
      661 ccattgtgtt tccgacgaaa accgggtgta ttctggagga agaagagaaa tttaaagacg
      721 cacacaggag cgtgcttgcg gaaacaagag cattgaggga ccggacaaga gaagagtcag
      781 gaaacaatgc cgcggaaatt attgatatac aatgctcctt gttaaaggat aacggactga
      841 tcgatccgat cagaaaagaa atagcaggag gaaccggagc tgcagctgcc gtagagaatg
      901 tgatgaatgg tttcatcaaa cagtttgagg gaatggaaaa tcagtatttt gcccaaaggg
      961 cggtagatct gaaggatctg aaaaacaacc tgatctataa aatcctgaat attccgcgtg
     1021 tggatattac gatgcttgcg gaaccgacga ttatcgccgg aaatgatatt acgccgtctc
     1081 aaacggccag gatggatcag catatggttt ccggactgct gatggaattt ggagggaata
     1141 cttcacatac tgcgatactt gcaagaacat tggatattcc tgcgatcgtg ggaatcaaag
     1201 ggctgctgaa agaaataaaa acaggtgata tggttggatt tgacggtgaa accggagaaa
     1261 tatttacaaa tttgacaaaa gtagaaacag cttctctgca aacaaaaata caggaagaaa
     1321 agcagagaaa agaaaggctg caggtgcttg cagccggcag ggcagcgacg ctcgacggaa
     1381 agctgct
//
LOCUS       DAOSYE010000713         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86343_length_1385_cov_2.724060, whole genome shotgun sequence.
ACCESSION   DAOSYE010000713 DAOSYE010000000
VERSION     DAOSYE010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86343_length_1385_cov_2.724060"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..>1385)
                     /locus_tag="PLU43_10835"
     CDS             complement(148..>1385)
                     /locus_tag="PLU43_10835"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO28948.1"
                     /translation="AKARMQIAVPAAIFIIFIILNALIVKADKYENTTLKVDSLNKMS
                     SVFFRSNFDSTIYYVKQAIKLSNESDYLEGKIKALSIWMSLKCSVVNIPYGGAKGGIK
                     VDPSTLSKRELCRLTRRYTFAIAPIIGADTDIPAPDVNTNSQTMAWILDTYSQLAGKP
                     SPGVVTGKPVELGGSRGRNSATGRGVYISTKLLLAQYGKKPEDVTVAIQGMGNVGSNA
                     ALIFYHRNTKIIAVNDISGGIYCPEGLDIETINERIKNEHLLLKDYDVPGVTHITNEE
                     LLTCKCDVLIPAALENQITGENADKLQCSYIVEAANGPTTEAADIILEKRNIPVVPDI
                     FANSGGVIVSYFEWVQNIQELTWERDRVNQMLENLMSKSFTEVTDIQNECHCSLRIAA
                     YITALKKLIYAEQIKGVFP"
BASE COUNT          364 a          283 c          322 g          416 t
ORIGIN      
        1 taaaggaagc ggacagggaa aagttattac ttgcattcga ctgtcatatc gaatgtatca
       61 aagagcatat ttcataggaa attttacaac ggcgtaaaca gaggttctgt ttacgccgtt
      121 tttttcatag gaaagaccca aatgttttta ggggaagaca cctttgatct gttctgcgta
      181 gatcagtttt ttcaaggcag taatataggc agcgatccta agtgaacaat ggcattcgtt
      241 ttgaatatca gtgacttccg taaaggattt tgacatcaga ttttcgagca tctgattgac
      301 ccggtcacgt tcccatgtaa gttcctgaat attctgcacc cattcaaaat aggaaacgat
      361 gacgccgccg ctgtttgcaa aaatatccgg tacaacggga atgttccgtt tttccaaaat
      421 aatatctgcg gcttctgttg tcggaccatt ggcagcttcc acgatatagg aacattggag
      481 tttatccgcg ttttccccgg taatctggtt ttcaagggca gcaggtatca gtacatcaca
      541 tttacatgtg agtaattctt cgtttgtaat atgtgtaaca ccgggaacat catagtcttt
      601 taaaagcaga tgttcgtttt tgatgcgttc attgatcgtt tcgatatcaa gaccttccgg
      661 acagtagatg ccgccggaaa tatcattgac cgcaatgatt tttgtgtttc tgtgataaaa
      721 aatcaaggcc gcgttgcttc cgacattgcc cattccctgg atcgcaacgg tcacgtcttc
      781 cggtttttta ccatattgtg caagcaaaag ctttgtgctg atatagacgc cgcggccggt
      841 cgcggaattg cggcctctgg atccgccaag ttcaacaggt tttccggtga cgacaccggg
      901 agacggcttt ccggcaagct gactgtaagt atccaggatc caggccattg tctgtgaatt
      961 tgtgttgaca tcaggggcag ggatgtccgt atcggcaccg atgatcggtg caattgcaaa
     1021 cgtatagcgc cttgtcagcc ggcaaagctc tcttttggaa agtgtggagg ggtcgacctt
     1081 gatcccgcct ttggcgccgc cgtagggaat attaacgacg gaacatttga gtgacatcca
     1141 tatggaaagc gctttgattt ttccttccaa ataatcagat tcatttgata atttgatggc
     1201 ttgcttaaca taataaatcg tagaatcaaa attgctccta aagaaaacac ttgacatttt
     1261 gttaagtgaa tcaactttga gtgttgtgtt ttcgtatttg tcggctttta caattaatgc
     1321 atttagaatg atgaaaataa taaagatagc ggcaggcacg gcgatctgca tgcgggcttt
     1381 ggcct
//
LOCUS       DAOSYE010000714         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86451_length_1384_cov_2.629044, whole genome shotgun sequence.
ACCESSION   DAOSYE010000714 DAOSYE010000000
VERSION     DAOSYE010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86451_length_1384_cov_2.629044"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLU43_10840"
     CDS             <1..412
                     /locus_tag="PLU43_10840"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO28949.1"
                     /translation="ALGSDFEVELSHHYGIPHFRQYGAIIINIINREYCKKYIIVLPG
                     QKHPAHAHRVKEETFQVLYGDLIIQIGNGEVKHLKPGDMQTVLRGEYHSFSSTTGAIF
                     EEVSTQHMKSDSYYQDQSINELDPMERKTYLKKW"
     gene            500..>1384
                     /locus_tag="PLU43_10845"
     CDS             500..>1384
                     /locus_tag="PLU43_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOO28950.1"
                     /translation="MSGNQRRERIKYWSQLLLLPIYGFSFFVPRNKKLWLFGSSFGNR
                     FAENPRYFYLYVKQNCPQIHAVWISHDKELVAFLNRHHYEAYEYHSLKGIFYAFRAGF
                     YIFDNYSKDINFWQSGGAVKINLWHGSGNKRTNHDNAFDKVRHPKTLFERWKTFPRRL
                     SDEKPGHYTLATSPAMRDIFISAFQTDRDHIIVEGYPRNDMLFSYEECHIRNLLTDSE
                     QELYQAIMAYKKNGFRILAYMPTFRDSEKKFFQVMDLDVFNCFLEKEKLILVTKIHPK
                     SKLKKEFQEIDCSNIWYAD"
BASE COUNT          437 a          255 c          311 g          381 t
ORIGIN      
        1 cgctcttggc agtgattttg aagtagagct ttcccaccat tacgggattc ctcatttcag
       61 acagtatggg gcgatcatta tcaatattat caacagggaa tactgcaaaa aatatattat
      121 tgttcttccg ggacagaagc atcccgcaca tgcgcatcgg gtaaaggagg agacattcca
      181 ggtactttac ggagacttga tcatccaaat cggaaacgga gaagtaaagc atttgaagcc
      241 cggagacatg cagactgtgc ttcgcgggga atatcattcg ttttcgtcta caacgggggc
      301 catttttgag gaagtgtcta cccagcatat gaagagcgat tcctattatc aggatcagtc
      361 catcaatgag ctggatccta tggagagaaa gacttatctg aaaaaatggt gaaagcttaa
      421 gaattatata aaatcagccg atataaatac taacagatat tgatttacga aatcgaacgg
      481 agtcgggcgg gaatcgaata tgagtggaaa tcaacgaaga gagagaatca aatattggtc
      541 acagctgctg ctattgccga tatatgggtt ctcttttttc gtaccgagaa ataaaaaact
      601 ctggctgttt ggaagctcat tcggaaaccg gtttgcagaa aatccgcgtt atttttattt
      661 gtacgttaaa cagaactgcc cgcagatcca tgcggtatgg atttcccatg acaaagagct
      721 ggtagcgttt cttaaccgac atcattatga agcatatgaa taccattccc tgaaagggat
      781 cttctatgcg ttccgggcag ggttttatat ttttgacaat tattcaaaag acatcaattt
      841 ctggcagtcc ggcggagcag ttaagatcaa tctctggcat ggttccggaa acaagcggac
      901 aaatcatgac aatgcatttg ataaagtacg tcatccgaag actctttttg aaagatggaa
      961 aacgtttccg agaaggttat ccgatgaaaa accgggacat tatacgcttg caacgtcacc
     1021 ggcgatgcgg gacattttta tttcggcatt tcagacagat cgtgatcata ttatcgtaga
     1081 aggctatccg agaaacgaca tgctgttttc gtatgaagaa tgccacatca gaaatctgct
     1141 tacagattct gagcaggagc tttatcaggc aattatggca tacaagaaaa atggtttccg
     1201 gattcttgcc tatatgccga cattcaggga ttctgaaaaa aagttttttc aggtaatgga
     1261 tctggatgta tttaattgct ttttggaaaa agaaaagctg atactggtca caaaaataca
     1321 tccgaaatct aaattaaaaa aagaatttca ggagatcgat tgttcaaata tctggtatgc
     1381 ggat
//
LOCUS       DAOSYE010000715         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86528_length_1383_cov_3.838855, whole genome shotgun sequence.
ACCESSION   DAOSYE010000715 DAOSYE010000000
VERSION     DAOSYE010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86528_length_1383_cov_3.838855"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLU43_10850"
     CDS             <1..868
                     /locus_tag="PLU43_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOO28951.1"
                     /translation="EMSKMDCYIGVRGTNNVAELSDVPSEKLAMYDLLYSTPVHHKIR
                     VPKTRWVVLRYPNDAMAQLSGSSTEAFEDFYFKVCNLDYSKMGEAMKNLISYMDRTDK
                     VRLVAKGTDLTFSIKGIPAIACDGKMNIPDGEVYTAPVRESVNGTISYNTPSLYQGFT
                     YENISLTFENGKIIRALSNNEEKINKVFDTDEGARYIGEFAIGVNPYITKPMKDILFD
                     EKIMGSIHFTPGNCYDEAPNGNKSAIHWDLVLIQTPEYGGGEIYFDDVLIRKDGRFVV
                     SELECLNPENLI"
     gene            complement(934..>1383)
                     /locus_tag="PLU43_10855"
     CDS             complement(934..>1383)
                     /locus_tag="PLU43_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOO28952.1"
                     /translation="ALGLSNGVIQVRFSEALTILPYFTPLAIPGLFVGCLISNLLTGC
                     MPLDVIFGSLATLLGAIGTGFIGKLSRKQAGSKKDSTIKWLAPIPPIVANTLIVPFIL
                     AYVYQFEGSIPYFMLTVGLGEVISCGILGMILLFSLQKHAAVLFKNS"
BASE COUNT          416 a          284 c          343 g          340 t
ORIGIN      
        1 tgaaatgtca aaaatggatt gttatatcgg cgttcgcgga acaaataatg tagcagagct
       61 ttcggatgta ccgtctgaaa agctggcaat gtatgacctg ttgtattcga caccggtaca
      121 ccataaaatc agggttccga aaacccgctg ggtcgtactg cggtatccga atgatgcgat
      181 ggcgcagctg tccggcagca gcacggaagc atttgaggat ttttatttta aagtatgcaa
      241 tctggattac tccaaaatgg gagaggccat gaaaaatctg atcagctata tggatcgtac
      301 ggataaagta cgcctcgttg cgaaaggaac ggacctgaca ttttccatca aagggattcc
      361 ggcgatcgca tgtgacggaa aaatgaatat tccggacgga gaagtctata cggcacctgt
      421 cagagagtct gttaacggaa cgatcagcta taatacgccg tctctgtacc agggctttac
      481 ctatgaaaat atcagcctga cgtttgaaaa cgggaagatc atcagggctc tttcgaataa
      541 cgaagagaag atcaacaagg tatttgatac ggatgaagga gccagataca tcggagaatt
      601 tgcgatcggc gtgaatccgt atattacaaa gccgatgaaa gatattctat ttgatgaaaa
      661 gatcatgggt tcgatacatt ttacgccggg gaattgctat gacgaagctc ctaacggcaa
      721 taaatctgcg atccactggg atcttgtact gatccagaca cccgaatacg gcggcggaga
      781 aatctatttt gacgatgtgc tcattcgcaa ggacggcagg ttcgttgttt cagaactgga
      841 atgcctgaat cctgaaaatt taatataacg gaaatttaaa actgccccgt acagtgtata
      901 tctgtacagg gcagttttta tatgcaaata tattcagctg tttttaaaca aaacagctgc
      961 atgtttttgc agggaaaata ataagatcat ccctaatatg ccgcaggaga tcacttctcc
     1021 gagtccaaca gtcagcataa aatatggaat ggatccttca aactgataga cataggcaag
     1081 tataaacgga acgatcagcg tatttgcgac gatcggcgga atgggagcaa gccattttat
     1141 ggtgctatcc tttttactgc ctgcctgttt tctggaaagc ttcccgatga atccggtacc
     1201 gatcgcacca agcagtgttg ccagactgcc gaagatcaca tcgagcggca tacagccggt
     1261 cagcagatta ctgatcagac atccgacgaa cagtcctgga atagcaaggg gtgtaaagta
     1321 gggaaggatc gtaagtgctt ccgaaaagcg gacctggatg actccgtttg agagcccgag
     1381 tgc
//
LOCUS       DAOSYE010000716         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86652_length_1382_cov_2.697061, whole genome shotgun sequence.
ACCESSION   DAOSYE010000716 DAOSYE010000000
VERSION     DAOSYE010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86652_length_1382_cov_2.697061"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..348
                     /locus_tag="PLU43_10860"
     CDS             73..348
                     /locus_tag="PLU43_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28953.1"
                     /translation="MTEIFEDKKVWLFGILCVAAGVALGLLFAPLTHGIRISLWSNNA
                     LGSGKLVSYMSEGGSNSHKYTADIKDDTKTAGEPETGKRKHGRGRNR"
     gene            393..>1382
                     /locus_tag="PLU43_10865"
     CDS             393..>1382
                     /locus_tag="PLU43_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOO28954.1"
                     /translation="MKITLKDGSAKEYDQAMSVLEIAEDISGGLARIACAGEIDGKRV
                     DLRTIVDRDCSLNILTFSDKGGADAFRHTTSHIMAQAIKRLYPEAKLAIGPSIDDGFY
                     YDIDSEEPITSDDFEKIETEMKKIVKENIAIERFTLPRADAIKFMQEKEEPYKVELIQ
                     DLPEDAVISFYKQGDFTDLCAGPHLMTTKPVKAIKLLKVAGAYWRGNEKNKMLTRIYG
                     TSYTKAADLEEYITRIEEAKKRDHRKLGKELGLFMLADEGPGFPFFLPKGMILKNLLI
                     DYWREIHTREEYVEISTPIMLNRQLWETSGHWDHYKDNMYTTVIDEEDFAIKPM"
BASE COUNT          460 a          232 c          354 g          336 t
ORIGIN      
        1 cgcggcagca tgtaagggac ttttgtattt ccgggaattt tgcggtatcg taatttataa
       61 ggaggatgaa agatgacaga gatatttgaa gataagaaag tttggctttt tggaattctt
      121 tgtgttgcag caggagtcgc acttggtttg ctctttgcgc cgctcacgca tggaatccgt
      181 atttctttat ggagcaataa tgcgcttggc tcaggaaaac ttgtttctta tatgtcggaa
      241 ggcggaagca acagtcataa atatacggca gatataaagg acgatacaaa gacagccggc
      301 gaaccggaaa cggggaaaag gaaacatggc cgcggcagaa acagataaat aaattcaatt
      361 tataaacaaa aagaatacag aggagactag tgatgaaaat tacattaaaa gacgggtccg
      421 ctaaggaata tgatcaagcg atgagtgtgc ttgagattgc ggaggatatc agcggaggac
      481 ttgcaagaat tgcctgtgca ggagaaattg acgggaagag agttgatctg cggacgatcg
      541 tggacagaga ctgcagttta aatatactga catttagcga caaaggcggt gccgatgcct
      601 ttcgtcatac gacttcacat attatggcac aggcgatcaa gcgtctttat ccggaagcta
      661 agctggcgat cggcccgtct atcgatgacg gattttatta tgatattgac agtgaagagc
      721 cgatcacttc cgatgatttc gaaaaaatag agaccgaaat gaaaaagatc gtaaaagaaa
      781 atattgcaat cgagcggttt acacttccaa gagccgatgc aattaagttt atgcaggaaa
      841 aagaagaacc atataaggtc gagctgattc aggatctgcc ggaggatgcg gtgatcagtt
      901 tttataaaca gggcgatttt accgacttat gtgcaggtcc tcatctgatg acgacaaaac
      961 cggtgaaagc gataaaactt ctcaaagttg ccggagctta ttggagaggc aatgaaaaaa
     1021 ataaaatgct gacaagaatt tatggaacct cttatacaaa ggcagcggat cttgaggaat
     1081 atattacaag aattgaagaa gcaaaaaaac gagaccatag aaagcttgga aaagaacttg
     1141 gactttttat gctggcagat gaaggcccgg gatttccgtt ctttcttccc aagggaatga
     1201 tattaaaaaa tttactgatc gattactggc gggagatcca tacaagggag gaatatgtgg
     1261 agatctccac accgatcatg ctgaacagac agttatggga aacatcggga cactgggatc
     1321 attacaaaga taatatgtat acgactgtga tcgatgaaga ggattttgcg atcaagccga
     1381 tg
//
LOCUS       DAOSYE010000717         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_86768_length_1381_cov_2.233032, whole genome shotgun sequence.
ACCESSION   DAOSYE010000717 DAOSYE010000000
VERSION     DAOSYE010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86768_length_1381_cov_2.233032"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLU43_10870"
     CDS             complement(<1..312)
                     /locus_tag="PLU43_10870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOO28955.1"
                     /translation="MKFQVLLSAMFLMDETYADTLHITSDAVIINQCDRTGRKEIDRK
                     NAVGREQMITYIETTQRGLSKSRNMAVSNAEAEVCILCDNDVEYVENYEKILRFKYDY
                     EA"
     gene            complement(316..1065)
                     /locus_tag="PLU43_10875"
     CDS             complement(316..1065)
                     /locus_tag="PLU43_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO28956.1"
                     /translation="MNEDMVSVIIPVYNAAKCISDTVQSVRSQTYTNWELILVDDGST
                     DRSVEIMQEFANDDIRLIRTDGGKSAALARNRGIMEAKGRYLAFLDADDLWNPDKLEQ
                     QVAFMKREAVAFSFTGYEFADEEGVGVQKIVKVPAKLKYGAALKNTTIFTSTVMFDLK
                     KISREAVMMPNVASEDTATWWKILKNGYTAYGLNESLTLYRRGSGTLSSNKVIAVKRI
                     WNLYRNVEHLSVPKSCYCFCFYAVRAVIRRM"
     gene            complement(1058..>1381)
                     /locus_tag="PLU43_10880"
     CDS             complement(1058..>1381)
                     /locus_tag="PLU43_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO28957.1"
                     /translation="SGSMSFVGPRPERPEIIEKYTEAMPEFTYRTKVKAGITGYAQIY
                     GKYNTRPYDKLKLDLYYIENFSIWMDLKLLIQTVKIIFTLESTEGIEDGNVTPLEREE
                     EEEDE"
BASE COUNT          348 a          380 c          243 g          410 t
ORIGIN      
        1 ggcttcataa tcgtatttga aacgcagaat cttttcatag ttttccacat attctacatc
       61 attatcacag agaatgcaaa cctccgcttc agcattggaa acagccatgt tccggctttt
      121 acttaagccc ctctgcgttg tttcgatata ggtgatcatt tgttccctgc cgacggcatt
      181 ttttcgatcg atctcttttc tgcctgtacg atcacactga ttaatgataa cggcatcaga
      241 agtaatatgc agagtatccg catatgtttc atccataaga aacatggccg acagcagcac
      301 ctgaaatttc atatcctaca tcctccgaat gactgcgcgc accgcataaa agcaaaaaca
      361 gtaacagctt ttcggaacag aaagatgctc tacgttccga tacagattcc agatcctctt
      421 taccgcaatg accttattgg aggaaagcgt tccgcttccc cttctgtata gcgtcagaga
      481 ttcattcaga ccatacgctg tataaccgtt ctttaatatt ttccaccagg tagccgtatc
      541 ctcgcttgcc acattcggca tcataacagc ttccctggat atcttcttta aatcaaacat
      601 gaccgtactt gtaaaaatcg tcgtattctt caaagcggct ccatatttca gctttgccgg
      661 aactttgacg atcttctgta caccaacacc ttcttcgtct gcgaattcat accctgtaaa
      721 actgaatgcc actgcttctc ttttcataaa agcgacctgc tgttccagct tgtccggatt
      781 ccacaggtcg tccgcatcca aaaatgccag atatctgccc ttcgcttcca taataccgcg
      841 atttctcgca agcgccgcac tctttccccc atccgtacgg atcaggcgga tatcatcatt
      901 ggcaaactcc tgcatgatct ccacactgcg gtccgttgaa ccatcatcaa cgaggatcag
      961 ctcccagttc gtataggtct ggcttctgac ggactgcacc gtatcactga tacattttgc
     1021 cgcattatat accgggatta tgacactgac catgtcctca ttcatcttcc tcttcctctt
     1081 ctctctcaag cggcgtaaca ttgccgtcct caattccttc cgtactttca agcgtaaaga
     1141 taatttttac ggtctggatc agtaatttca gatccatcca tatcgaaaaa ttttcaatgt
     1201 aatacagatc cagcttcagc ttgtcataag gtcttgtatt atactttccg taaatttggg
     1261 cgtatccggt aattcccgcc ttgactttcg tacgataggt gaattccggc attgcctcgg
     1321 tatacttctc aataatttcc ggacgctccg gacggggccc gacaaacgac atcgaaccgg
     1381 a
//
LOCUS       DAOSYE010000718         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_87027_length_1378_cov_3.850340, whole genome shotgun sequence.
ACCESSION   DAOSYE010000718 DAOSYE010000000
VERSION     DAOSYE010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87027_length_1378_cov_3.850340"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..777
                     /locus_tag="PLU43_10885"
                     /pseudo
     CDS             52..777
                     /locus_tag="PLU43_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            725..>1378
                     /locus_tag="PLU43_10890"
     CDS             725..>1378
                     /locus_tag="PLU43_10890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO28958.1"
                     /translation="MEENLIPERTEKEETADNKTVGGEKKFGRLKAIGYTLLMILLFY
                     LIQFAITVPIIIVYAFQYIAGIDAPIDMQSFIASITENGLITIVTAVSTVATTLVFAL
                     WYYFKYGRLQKRSLPELWGNAFGVKKTILYIVGSLFCYFMASNTISGIDLISPSSVDA
                     YTEMMQQISSGSQGLLLLAVVILAPIGEECLFRGLVFQRFRKYFTIVAAWILQAVFFG
                     "
BASE COUNT          346 a          249 c          378 g          405 t
ORIGIN      
        1 cgtttcccgt gattcggacc agtgtcggtt acagacaggg atattgcgga taaatgtatc
       61 aggctcggca ttcctgttgc ggcgcagtcg tcgctgatcg caatttcctg tgtggctctg
      121 cagggtgttg taaatcagtt tggtgctgat gttgtagccg cctttacggc aacttcaaga
      181 attgagcagc ttgtacagca gccttttaat tctttaggtg ctgcggtttc tacttttacg
      241 ggccagaata tcggcgcaag ggaacttgca cgcgtaaaaa gaggatatta taagagcgtt
      301 attattgtag cggtcttcag tattctgatg ctggctgtgg cgtatctgtt tgggaatgcc
      361 attatgcggc tgtttgtaaa aaacgaagag gtcataagca ttggagcgaa ggctctgcgc
      421 attacaagtc ttatgtattt tccgcttggt atgatctatg tgacaagagg tctgctgaac
      481 ggcgccgggg atgcttttta tgcgatgatc aacggactga tcgaggttgc gggccgtatc
      541 ggatttgcca atacgcttgt atggatttca acgatcggcg tatgggggat ctggtgggca
      601 accggactta catgggtcat cacgggagct gcgagtgtta ttcgctatcg gcagggcagg
      661 tggaagcgga tgtcggtagt ggaaaaagag catgcatggc acaaaatacg gagaggagca
      721 gaagatggag gagaatttaa taccggaaag aacagaaaaa gaagaaactg cggataataa
      781 gacagtggga ggggaaaaga aattcggtcg gctgaaagcg atcggctata cacttcttat
      841 gattctgctt ttttatctga ttcagtttgc aattacggtt ccgatcatca ttgtttatgc
      901 atttcagtat attgccggaa ttgatgcacc gatcgatatg cagtcgttta ttgcatcaat
      961 taccgagaac ggcctgatta cgattgtgac cgccgtgtcg acggttgcca cgacgcttgt
     1021 atttgctctt tggtattatt ttaaatatgg gagattacaa aaaagaagtc ttccggaatt
     1081 atggggaaat gcgttcggcg taaaaaaaac gattctttat atcgtaggtt ctttattttg
     1141 ttatttcatg gcatccaata ctatttcagg cattgatctg atatctccgt cgtccgtaga
     1201 tgcatatacc gaaatgatgc agcagatatc aagcggaagc cagggactgc tgcttttggc
     1261 tgttgtgatt ttagcgccca taggagagga atgtcttttc agaggattag tatttcaaag
     1321 attcagaaag tattttacga ttgtcgcggc atggatcctg caggccgtat ttttcggg
//
LOCUS       DAOSYE010000719         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_87291_length_1376_cov_2.666919, whole genome shotgun sequence.
ACCESSION   DAOSYE010000719 DAOSYE010000000
VERSION     DAOSYE010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87291_length_1376_cov_2.666919"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1254
                     /locus_tag="PLU43_10895"
     CDS             355..1254
                     /locus_tag="PLU43_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO28959.1"
                     /translation="MYEIIDEFCWTKEKKIITKEKHQVPGLCNFSHYTHTHSTPPAPL
                     HYHSNIIEIHCMIKGSLYNQIEKDGLLTQYVTMGNQAFISYPFEIHGNGSLPLPPHEF
                     FAFQINISNPADLLGLNKEYSYSLYKQLIQLPYHQLCLGATHINNLRSAYNFFSELTP
                     EATKIGVQFLTCFLFNLKFLTPISETTVSGIDAPIKKAIDYMTKNISEDLHVSDLASI
                     SGYSLSRFKVKFKSEIGVTPAEYINLQKIEAAKKQLTASNCSITDIAYSLGFSSVSYF
                     SSVFKKLTSCTPQHYRKNYSSND"
BASE COUNT          420 a          311 c          206 g          439 t
ORIGIN      
        1 caaaacgctc agccagaatc tgctctgacg gcagtctgtc attttcctgc atgtgttcat
       61 taataatgat ctcacgcaga ttctcggcaa tttgttcata taaatgtgac ctgattactt
      121 tataattttc ttctgccatt gacatcctcc tgtccacctg ttctacagct atactatcat
      181 aagatgtttt tttttgctta taatttaatc ctcaatactt ttttaatact tttttataat
      241 tatgaaaata acacgaattt catatttcag atagaaaatt gcactttatt tttacagatt
      301 ttcatgataa gttatagata tgccgttatt gaaacaacta tgataaggag tcccatgtac
      361 gaaattattg atgaattctg ctggacaaaa gaaaaaaaga tcataacaaa ggaaaagcat
      421 caggttcccg gtctttgtaa tttctcacat tacacacata cccactctac accgccggct
      481 cctttgcatt atcattccaa tatcattgag atccattgca tgatcaaagg atctttatat
      541 aatcagatag aaaaagacgg gttgcttaca caatatgtca caatgggaaa tcaggctttt
      601 atctcctatc cgtttgaaat tcatggaaat ggcagtctcc ctctcccgcc gcatgaattt
      661 tttgcttttc agatcaatat ttccaaccct gccgatctgc ttggactaaa taaggaatac
      721 agctattccc tatataagca gctgattcag ttaccatatc accagctctg tctcggcgca
      781 acgcatataa acaatttaag aagcgcctat aattttttca gtgaactgac accggaagcc
      841 acaaaaatcg gcgtacagtt cctgacctgt tttttattca atctaaaatt cctgacgcct
      901 atttcagaga caaccgtatc cggcattgac gctcccataa aaaaagccat tgactatatg
      961 acgaaaaaca tttcagaaga tctgcatgtc agtgaccttg cttccatttc cggttattcc
     1021 ctgtctcgtt tcaaggtgaa attcaaaagc gagatcggcg ttactcccgc cgaatatata
     1081 aatctgcaga aaatagaagc cgccaaaaaa caattgaccg cttctaactg cagtatcaca
     1141 gatattgcat attccctcgg attttcttcc gtcagttatt tcagttccgt gtttaagaaa
     1201 ctcacctcct gtactccgca gcattataga aaaaactata gttccaacga ttaatcaact
     1261 gaaaatcgtt attccccatc tattatctta aagacaacgg cacaggcaga agctttatga
     1321 tctgttaccc tgatttctat tccattctct ttctgcacaa aagatgcttt cgtatc
//
LOCUS       DAOSYE010000720         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_87326_length_1376_cov_1.889478, whole genome shotgun sequence.
ACCESSION   DAOSYE010000720 DAOSYE010000000
VERSION     DAOSYE010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87326_length_1376_cov_1.889478"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLU43_10900"
     CDS             <1..325
                     /locus_tag="PLU43_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28960.1"
                     /translation="NVSGGQRQRLAIARALIRKPEIYIFDDSFSALDFKTDAALRKAL
                     YQEIENSTVLIVAQRVSTIMHADRILVLDNGELAGIGTHQELLENCGIYREIVYSQHK
                     ESEVG"
     gene            325..>1376
                     /locus_tag="PLU43_10905"
     CDS             325..>1376
                     /locus_tag="PLU43_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO28961.1"
                     /translation="MKEEHTHAKMPPHYGRRGHGGPGGPPGMVPGEKAKDFKGSFKRL
                     MSYIGVYKNAMIIVIFMAVASSVFGILTPKVLAGAMDILYDGFIAGTKGAAISIDFSE
                     IRNILFLLGALYLLNAVFRYFQQYIMADIGQKVIFRMRKEVDEKLTRLPIKYFDDNSR
                     GDILSRMSNDIDNIGNSLQQASVQIISSVITIAGVFIMMLSISPFMTLLVLLTLPLIM
                     FVTMVIAKRSQKYFSRQWETLGELNGHVEEMFTGADVVKAYGYEKKAIEKFTEENTRL
                     YEQSRKAQFISGVIMPLINAIGNLSYVLICIAGGLKVINGTLSFSGVTAFIQYQKQFS
                     QPITQTANMMNVFQSAL"
BASE COUNT          387 a          264 c          351 g          374 t
ORIGIN      
        1 aaatgtatcg ggcggccagc gtcagcgtct ggcgatcgca agagcactga ttcgaaaacc
       61 ggaaatttat atttttgacg atagtttttc agcacttgac tttaagaccg atgctgcgct
      121 tcgcaaggca ttatatcagg aaatcgaaaa cagtacggtg ttgatcgttg cccagcgtgt
      181 cagcacgatc atgcatgcgg acaggatcct tgtacttgat aacggagagc tggccggtat
      241 cggcacacat caggaattgc ttgaaaactg cggaatatac agggagatcg tatactcaca
      301 gcacaaggaa agtgaggtgg gttaatgaaa gaagaacata cgcatgcaaa aatgcctcct
      361 cattacggca gaagaggaca tggcggtccg ggcggaccgc ccggcatggt gccgggagaa
      421 aaggccaaag attttaaagg aagcttcaaa cgtctgatga gttatatcgg cgtatacaaa
      481 aatgccatga tcattgttat ttttatggct gttgcgtcgt ctgtttttgg tattctgaca
      541 cctaaagtac tggccggtgc catggatatt ttatatgacg gttttatcgc aggaactaaa
      601 ggtgctgcaa tatcgatcga tttttccgaa atcagaaata tattgttcct gttgggagct
      661 ctttatttgc tgaatgccgt attcagatat tttcagcagt atattatggc agatatcggt
      721 cagaaggtca tctttcggat gagaaaggaa gtagatgaaa aactgacaag gctgccgatc
      781 aaatattttg atgataattc caggggtgat attttaagcc gtatgagcaa tgatatcgac
      841 aatatcggaa attccctgca gcaggcatca gttcagatca tctcgtcggt cattacgata
      901 gcaggtgtgt ttattatgat gctgtccatc agtcctttca tgacactgct cgtactcctg
      961 acgcttccgc tgatcatgtt tgttacgatg gtcatagcga agcgctcaca gaaatatttt
     1021 tccaggcagt gggaaacgct cggtgagctg aacgggcatg tggaggaaat gtttacaggg
     1081 gcagacgttg taaaagcgta tggctatgaa aagaaagcaa tagaaaaatt tacggaggaa
     1141 aatacccgtc tttatgagca aagccggaaa gcacagttta tttccggtgt gatcatgccg
     1201 ctgataaatg cgatcgggaa tctgtcttat gtgttgatct gtattgcggg cggtctgaag
     1261 gtcatcaacg gtaccttgtc gttcagcggt gtgacggcat ttattcagta tcagaaacaa
     1321 ttttcgcagc cgattacaca aacggcaaac atgatgaatg tgtttcagtc tgcact
//
LOCUS       DAOSYE010000721         1373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_87590_length_1373_cov_2.716237, whole genome shotgun sequence.
ACCESSION   DAOSYE010000721 DAOSYE010000000
VERSION     DAOSYE010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87590_length_1373_cov_2.716237"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLU43_10910"
     CDS             <1..534
                     /locus_tag="PLU43_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28962.1"
                     /translation="ETAQTANELLKKNLSVISVARNDKKILPEKDHASDRAGDREGIG
                     YTVPVTYDFKKEQSVFKEAERKPQTEEPEQSRIQPLFSRIKEEAADDAALTLTSEADI
                     MKPIPKKQEQLMDSTAMSSALQQKKQPVKGSTRMGENNNQRILELYGQKKSYVEIARE
                     LNLGVGEVKLVIDLFKG"
     gene            544..1104
                     /locus_tag="PLU43_10915"
     CDS             544..1104
                     /locus_tag="PLU43_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28963.1"
                     /translation="MKLKLYLRGLGIGVIVTALLMGFSGSGKQTLTDDEIIAKAKALG
                     MVQEDQVLLSPEAENSDADVESADSAISSAQAVEEINTAEAEPSEEEADAADTEQSEE
                     EADAVDTEQSEDQAEGEPYVIEIAKGSSSDTVSALLQKGGLISDAADFDSYLCRYGYD
                     HRIVSGKHEIPSGADYETIAKLITTR"
     gene            1182..>1373
                     /locus_tag="PLU43_10920"
     CDS             1182..>1373
                     /locus_tag="PLU43_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO28964.1"
                     /translation="MGDYILSCSSTADISEEHFKERNISFICFHFELDGKDYKDDLGK
                     SVPFDQFYRAMENGAETRTS"
BASE COUNT          427 a          234 c          389 g          323 t
ORIGIN      
        1 gaaaccgcac agaccgcgaa tgagctttta aaaaagaatc tgagcgtgat ttccgttgcc
       61 agaaatgata aaaaaatcct cccggagaag gatcatgcgt ctgacagggc aggagacagg
      121 gaaggaatcg ggtatacggt tcctgttacg tatgatttca aaaaagaaca gtctgttttt
      181 aaggaagcgg agcgcaaacc tcagacagag gaacccgaac agtccaggat acagcctttg
      241 ttttccagaa tcaaagaaga agcagcggat gatgcggctc ttacgcttac gtctgaggcg
      301 gatatcatga agccaattcc gaaaaaacag gaacaattga tggatagtac ggcaatgtca
      361 agtgcgctgc agcagaaaaa gcagccggta aagggcagta cgcgaatggg agaaaataac
      421 aatcaaagga ttctggagct ttacgggcag aaaaaatcct atgtggaaat tgcgagagag
      481 ctgaatcttg gtgttggcga agtaaagctt gtcatagatt tgtttaaggg ttaagggaaa
      541 agcatgaagc taaaattgta tttgagaggt cttggaatcg gtgtgatcgt aactgccctg
      601 cttatgggat tttcaggaag cgggaagcag acgctgacgg atgatgaaat aattgcaaag
      661 gcgaaagcac ttggtatggt acaggaggac caggtactat tgtcgccgga agcagaaaac
      721 tctgatgcag atgtggaatc ggcggattct gccatatcct ctgctcaggc tgtggaggag
      781 atcaatactg cggaagcgga accgtctgaa gaggaagctg atgctgcgga tacagagcag
      841 tccgaagagg aagctgatgc tgtggataca gagcagtctg aggaccaggc ggaaggagaa
      901 ccctatgtga tcgagattgc aaagggaagc agctcagata cggtgagtgc gctgctgcaa
      961 aagggaggtc tgatatcgga tgctgcggat ttcgacagct atttatgccg atatggctat
     1021 gatcacagaa tcgtttcagg gaagcacgag attccgtcag gagcggatta tgagacgatc
     1081 gcaaagctga ttacgacccg atgaatcagc cgttttggtt gaactttgtt tggattaatg
     1141 atacaatact ttacacttgg gtatgaaagg atgagaaaat gatgggagat tatattttga
     1201 gctgcagttc tacggctgat atttctgaag aacattttaa agaacgaaat atttcattta
     1261 tttgttttca ttttgaactg gatggaaagg attataaaga cgatcttgga aagtcggttc
     1321 cgtttgacca gttttatcgc gcaatggaaa atggagcgga aacaaggact tct
//
LOCUS       DAOSYE010000722         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_87667_length_1372_cov_4.429765, whole genome shotgun sequence.
ACCESSION   DAOSYE010000722 DAOSYE010000000
VERSION     DAOSYE010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87667_length_1372_cov_4.429765"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1064
                     /locus_tag="PLU43_10925"
     CDS             222..1064
                     /locus_tag="PLU43_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO28965.1"
                     /translation="MEKSIKKYFPIFVLPTMIAFAIGFLAPFIMGIYLSFCEFTTVVD
                     AEFVGIKNYTRIFSDDTFEHALWFTAAFTIVSVIVINLLAFIVAMLLTKSFHGTNIFR
                     TVFFMPNLIGGIILGYIWQLILNGILLHWERTLTYSSAYGFWGLLILMCWQQVGYMMI
                     IYIAGIQNVPGDLIEAAKIDGATKWQTIKRVIIPMVMPAITICTFLTLTNSFKLFDQN
                     LALTAGEPSNMSEMLALNIYNTFYGRVGWEGVGQAKAVVFFLLVALIAVVQMRLTRTK
                     EVQQ"
     gene            1064..>1372
                     /locus_tag="PLU43_10930"
     CDS             1064..>1372
                     /locus_tag="PLU43_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO28966.1"
                     /translation="MEQKNKNALLTVIFSIFSILYVCPIIIVLINSFKKKTYISKYPF
                     QIPTGKQFVGIDNYVRGMEKINFWGSFLNTLIITVGSVTVIILCTSMCAWYITRVNNK
                     V"
BASE COUNT          380 a          245 c          322 g          425 t
ORIGIN      
        1 ttcttgaata tgcacaggga accagcgact gggatggtgt cgtaacagct tttgtagacg
       61 gatgggctac agaatatgca gcttcaaatg ctgctgagta atattgatga cagacgttta
      121 ggcattacag actgattttg tagggtgagc gggcaaatgg ctcacccttt attaaaaccg
      181 aaggcggctg accgcactcg gacaggcgga aagggaatgt tatggagaag tcaattaaaa
      241 aatattttcc catatttgta ttaccgacaa tgatcgcatt tgcgatcggt tttctggctc
      301 cttttattat gggaatctac ttgtcgtttt gtgaatttac aacagtggtt gatgcggaat
      361 ttgttggaat taaaaattat accaggattt tttctgatga tacatttgaa catgcattat
      421 ggtttacggc agcgtttacg atcgtatcgg taatcgtgat caatctgctg gcatttattg
      481 ttgcaatgct tttaacaaag agttttcatg gaacaaatat ttttcgtaca gtatttttta
      541 tgccgaatct gattggcgga attattttgg gatatatctg gcagctgatt ctgaatggaa
      601 tcctgctgca ttgggagcgt acgctcacat acagttctgc ctacggtttt tgggggttgc
      661 tgatcctgat gtgctggcag caggtcggct atatgatgat tatttatata gcagggattc
      721 agaacgtacc aggtgatctg atcgaagctg ccaagatcga tggagccaca aaatggcaga
      781 cgatcaaacg ggtcataatt ccgatggtca tgccggctat tacgatctgc acatttttga
      841 cgttgacaaa ttcctttaag ctctttgacc agaaccttgc actgactgct ggagaacctt
      901 ccaatatgtc agaaatgctt gcattgaaca tctataatac gttctatgga agagtcggct
      961 gggaaggagt cggacaggcc aaggcggtcg tattctttct gctggtagca ctgatcgcag
     1021 tcgttcagat gcgtctgaca aggacaaagg aggtgcagca gtaatggaac agaaaaacaa
     1081 gaatgcactt cttacagtga tcttcagtat tttttctata ttgtatgtct gccccataat
     1141 cattgtattg attaattcgt tcaagaaaaa aacttatatt tccaaatatc catttcagat
     1201 cccaacagga aaacagttcg ttggaattga taattatgtc aggggcatgg aaaaaatcaa
     1261 tttctggggt tcattcctga atacactgat cattacggtc ggttctgtga ctgtcattat
     1321 tctctgcaca tcgatgtgtg catggtatat tacaagggtc aataataaag tg
//
LOCUS       DAOSYE010000723         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_88120_length_1368_cov_3.313785, whole genome shotgun sequence.
ACCESSION   DAOSYE010000723 DAOSYE010000000
VERSION     DAOSYE010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88120_length_1368_cov_3.313785"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..884)
                     /locus_tag="PLU43_10935"
     CDS             complement(15..884)
                     /locus_tag="PLU43_10935"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28967.1"
                     /translation="MKGKKKFRSTILPAAIMVGPVTLWLLALVAIPLIYVVIMTFCGL
                     DENYNVVFRFTLDNYKRLLDANYVQIYGQSLLIAFLTTLFCIVLAYPFSFLISRVFTG
                     KKTVLYMLVIIPFWTNSLIRIYGWRTFLGTNGWLNLMLQKLHLVKEPVEFLFNRGTIV
                     LGMVYCLFPFMVLPLYTAIEKLDKNLIEASADLGARGAVTFLKVILPLTASGIFSGSI
                     MVFIPCLGYFYVADILGGGKSDVIGNLIERQFQSGNNWPLGAALSVILIAITLLLVKL
                     YQKCGGNMENLGV"
     gene            complement(917..>1368)
                     /locus_tag="PLU43_10940"
     CDS             complement(917..>1368)
                     /locus_tag="PLU43_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO28968.1"
                     /translation="PVEIYEEPATRFVASFIGETNLFDGMVSRVTDDLVQVEVESGLI
                     IGKGIGFDEGEMVAVSVRPERMQYSQEPVEGFSLQAVVKEQVYIGSVLKVIAILQNGN
                     EIKIERLAGQELPEDGVVYLYWKAEDAKMIHSFDDNIYTAIENIQLA"
BASE COUNT          384 a          370 c          261 g          353 t
ORIGIN      
        1 cttctgtcca tacgctacac ccccagattt tccatattcc cgccgcattt ttgataaagc
       61 tttaccaaaa gcagggtaat ggcaattaag atgaccgaca gggccgcacc aagcggccag
      121 ttgttgccgc tctgaaactg tctttctatc aggtttccga ttacgtccga cttgcctcct
      181 ccgagaatat cagctacata gaaataacca agacacggaa taaataccat gatcgaaccg
      241 gaaaagatac cgcttgccgt cagcggaagg atcaccttta aaaaagtaac cgctcccctt
      301 gcgccaagat cggcggaagc ctcgatcaga tttttgtcca gtttctcgat cgccgtataa
      361 agaggcagta ccataaacgg aaagagacag tataccatac cgagaacgat cgtccctctg
      421 ttaaacagaa attccacggg ttctttcaca agatgcagtt tctgcagcat cagattcagc
      481 cagccgtttg ttccaagaaa cgttctccat ccgtaaatac ggatcaggga attcgtccag
      541 aacgggataa tgacgagcat atacagcact gtcttttttc cggtaaatac cctggagatc
      601 aaaaacgaaa acggataagc cagcacaatg cagaacagcg tcgtaagaaa agcgatcagc
      661 agcgactgcc catagatctg aacataattg gcgtccagca gtcttttata gttatcaagc
      721 gtaaacctga atacgacatt ataattttca tccaggccgc agaatgtcat gatcacaaca
      781 taaattaacg gaatcgccac aagggcaagc agccacaatg tcacaggtcc gaccatgatc
      841 gcagccggaa gtatcgtgga gcgaaatttt ttctttcctt tcataaatca ttctcccttt
      901 ctgattatta ctgattttat gccagctgaa tattttcaat ggccgtataa atattgtcat
      961 caaatgaatg tatcatttta gcatcttccg ccttccagta gagataaacg actccgtctt
     1021 cgggcagctc ctgtcctgcc agcctttcga tcttgatctc attcccgttc tgcagaatgg
     1081 cgatcacctt cagtacggaa ccgatataga cctgctcctt tacgaccgcc tgcagggaaa
     1141 atccttctac aggttcctgc gaatactgca ttctttccgg tctgacagat accgccacca
     1201 tttcaccttc atcaaatccg attccttttc cgatgatcag tccgctttcc acttccacct
     1261 gtaccagatc atccgtcacc cggcttacca tcccgtcaaa cagatttgtc tccccgataa
     1321 aagaagctac aaatctggtc gccggttctt catagatctc taccggtg
//
LOCUS       DAOSYE010000724         1367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_88223_length_1367_cov_3.583079, whole genome shotgun sequence.
ACCESSION   DAOSYE010000724 DAOSYE010000000
VERSION     DAOSYE010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88223_length_1367_cov_3.583079"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLU43_10945"
     CDS             <1..210
                     /locus_tag="PLU43_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28969.1"
                     /translation="ANDLFRGAYNKIQEVGMEGNNIYALFPMLELGYKETAKHYMETF
                     GCAGKAWTARQYVSKESIPDLNEAK"
     gene            282..1064
                     /locus_tag="PLU43_10950"
     CDS             282..1064
                     /locus_tag="PLU43_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO28970.1"
                     /translation="MKITQISFIYFSPTGTTKGVLALLKNCFDLPVREYDLTDYPETE
                     HNMNFGSSDLVIFAFPVYSGRVLETFRERIGHIKGNHTLSAIVAAYGNREYDDSLLEM
                     ADISDAAGFLNIGAIAVVTEHSVIRSIGAGRPNEKDRDMILTFGEELLKRADELIGTE
                     EEVHLKLPGNRPYRRYEHIPLHPLSTSSCNGCLACVKTCPVEAITYDGQCHTDKKKCV
                     GCMKCIRICPQRARTNGKIKMMAAETFMKLVCRSEKQSELFL"
BASE COUNT          436 a          221 c          346 g          364 t
ORIGIN      
        1 gcgaatgatc tgttccgcgg agcatataac aagattcagg aagtgggaat ggaaggaaat
       61 aatatttatg cactgttccc aatgctggaa ttaggttata aggaaacggc aaagcattat
      121 atggagacgt tcggctgtgc aggaaaagca tggactgcaa ggcagtatgt ttccaaagaa
      181 tcgattccgg atctgaacga ggcaaaatag catggaaaaa gcataaagaa tatagtataa
      241 tgtcgtcagg ttttattata tgaaagactg gagcagggta aatgaaaatt acgcagatca
      301 gttttatata ttttagtccg accgggacga caaaaggagt gcttgctcta ttaaagaatt
      361 gttttgacct tcctgtcagg gaatatgacc tgactgatta tccggaaacg gagcacaata
      421 tgaactttgg cagttctgat ctggtcattt ttgcttttcc ggtctacagc ggccgggtac
      481 tggaaacctt tcgggaacga atcgggcata taaaaggaaa tcatacgctt tcggcaatcg
      541 ttgcagcgta tggaaaccgg gaatatgatg attccctttt ggagatggcc gatatttccg
      601 atgcagcagg atttttaaat atcggtgcca tagcagttgt gacggaacat tccgtaatac
      661 gttctatcgg tgccggaagg ccgaatgaaa aagacagaga catgattttg acttttggag
      721 aggaactgct gaaaagggca gatgagctca tagggacgga agaggaagtg catttgaagc
      781 tgccgggaaa cagaccatat cgaagatatg agcatatccc gctgcatcct ttaagtacat
      841 cttcatgcaa cggatgtctt gcctgtgtga agacatgccc tgttgaagcg attacatatg
      901 acggacagtg tcatacggat aaaaagaaat gtgtcggatg catgaaatgc atacggatat
      961 gtccgcagag agcaaggaca aacggaaaaa tcaaaatgat ggctgcagag acttttatga
     1021 agcttgtctg cagaagtgaa aagcagagtg aactgttttt atagataaaa gagtgtgtca
     1081 taaaagaatt cctggataat ttgcagttat ccaggttttt ttattataaa aaattttttt
     1141 taacctcaat ttcagcttta tctgttacgt atatataagt ataacaataa acttatttta
     1201 tacaggagga aaatgaaatg aagaaattga tcgtaaaaca tatggcaggg atgattataa
     1261 caggagcgct tgtattgact gccggaatcg gagtgtttgc tgctcctgcg aatggtgccg
     1321 gtgccggcaa tgctgcagga gagatgcctg caatgagtga aaatggt
//
LOCUS       DAOSYE010000725         1364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_88621_length_1364_cov_2.330787, whole genome shotgun sequence.
ACCESSION   DAOSYE010000725 DAOSYE010000000
VERSION     DAOSYE010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88621_length_1364_cov_2.330787"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLU43_10955"
     CDS             <1..277
                     /locus_tag="PLU43_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28971.1"
                     /translation="FTGPVNIGNPQEFTIRQLAEKVIELTGSSSQIVFQSLPQDDPLQ
                     RKPVIDLARKELDWEPKILLEEGLVKTIAYFRELIENNDRRLIRKEE"
     gene            complement(293..547)
                     /locus_tag="PLU43_10960"
     CDS             complement(293..547)
                     /locus_tag="PLU43_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28972.1"
                     /translation="MKRIGALIGAGLLALLYILTLVFALMKNPQAVGLFKASLAATII
                     IPCLIYAYQLIYRVLKRRGEELADEAKTAAGKTEHPDNTQ"
     gene            692..>1364
                     /locus_tag="PLU43_10965"
     CDS             692..>1364
                     /locus_tag="PLU43_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28973.1"
                     /translation="MIVFELYRQLDVRMYMTVAVMDRSYGSCAKYIMLGDTLLLLGTD
                     HSIKLNMLTREVTTDNPIRNWDIEAEPVMWFAEESPEAGMIRMINNAFRQWLEIDPFP
                     SYAAFSEAEHTINRKLSKYNLRIIFDMNSMCIYHDEDIITFEQALELIMEIDSEQESG
                     ASLIEWQQEAEYLKNTGQQEAAAKRFERIIRHTDRTQMIYTISTFQLAEIYYFLGNYE
                     RAVTLY"
BASE COUNT          391 a          275 c          348 g          350 t
ORIGIN      
        1 atttacgggg ccggtcaaca tcggcaatcc gcaggagttt acgatcaggc agcttgccga
       61 aaaagtgatc gaactgacag gcagctcttc acagatcgtt tttcagtcgc tgccgcagga
      121 tgacccgctg cagagaaagc cggtcattga tctggccaga aaagaacttg actgggagcc
      181 aaagattctg ctggaggaag gtctggttaa aacgatcgca tatttccggg aattgatcga
      241 aaataatgac cgccgcctga tcagaaaaga agagtaggcg gtacggcagg tattattgtg
      301 tgttgtcagg gtgttctgtt tttccggcgg ctgttttggc ttcgtcggcc agttcttccc
      361 cacggcgttt caggacgcgg tagatcagct ggtacgcata aataaggcat ggaataataa
      421 tggttgccgc aagcgatgct ttaaataagc cgactgcctg cggatttttc attaacgcaa
      481 ataccagcgt gagaatatat aaaagagcaa gcagtcctgc tccgatcaat gcgccgattc
      541 ttttcataaa tattctcctt ttttataata aatgataatg tttccgtatg tcacactgct
      601 gttttagtat atcacaaaat gatttttaca acaacaggcg acggaagccg ttatttttga
      661 ccaatgccgg acgggaggaa atgagacaaa aatgattgtg tttgaacttt acaggcagct
      721 ggatgtcagg atgtatatga cagtagccgt aatggacaga agctacggat cctgtgcaaa
      781 atatataatg ctcggcgata cgctcctgct tcttggaacg gatcattcca taaagctgaa
      841 tatgctgaca agagaagtga cgacggataa tccgatccgc aactgggata ttgaggcaga
      901 gccggttatg tggtttgcgg aagagtctcc cgaggccggc atgatcagaa tgatcaataa
      961 tgcgttcagg cagtggctgg aaatagatcc ttttccaagc tatgcagcat tttcagaggc
     1021 ggaacataca atcaaccgga aactgtccaa atataatctg cgtattattt ttgatatgaa
     1081 cagtatgtgt atctatcacg atgaggatat tattacgttt gagcaggcgc ttgagctgat
     1141 catggagatc gattccgaac aggaatccgg tgcatcgctc attgaatggc agcaggaagc
     1201 ggaatacctg aaaaatacgg gacagcagga agcggcggca aagcggttcg aacgcattat
     1261 caggcacacg gacaggacac agatgatcta cacgatcagt acattccagc tggctgaaat
     1321 ttattatttt ttgggaaatt atgaacgtgc cgttacactg tatt
//
LOCUS       DAOSYE010000726         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_88908_length_1361_cov_2.984686, whole genome shotgun sequence.
ACCESSION   DAOSYE010000726 DAOSYE010000000
VERSION     DAOSYE010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88908_length_1361_cov_2.984686"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLU43_10970"
     CDS             <1..773
                     /locus_tag="PLU43_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO28974.1"
                     /translation="TEHGSVTLRMTWQPLDGEMIALLIEVEDTGAGIKEEDIPKIYEK
                     FQRIDIEKNKNIEGTGLGMTITAQFVKMMDGEIRVRSQYGSGTCFNLKIPQKIRSQEV
                     IGSIQKSRERELAELKRDRVTFTAPKARILVVDDNEINLKVFEGLLRKTQIHIETAES
                     GAECLKLTEEQTYDLIFMDHMMPQMDGIEAFRRLKAQDTGKCRETPVIALTANAIVGA
                     REEYIAEGFTDYLSKPIDGIRLREMLLRYLPKQLIIKI"
     gene            796..1287
                     /gene="ispF"
                     /locus_tag="PLU43_10975"
     CDS             796..1287
                     /gene="ispF"
                     /locus_tag="PLU43_10975"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOO28975.1"
                     /translation="MRIGMGYDVHRLTENRELILGGVKIPFEKGLAGHSDADVVLHAI
                     MDALLGAAALGDIGKHFPDTEESYKDISSLVLLKRVGELLEENQYFIENIDATIIAQR
                     PKLRPYIDEMNKNIADVLGIALNQVNIKATTEEGLGFTGSGEGISSQAICMLKTAAIM
                     KNK"
BASE COUNT          422 a          241 c          384 g          314 t
ORIGIN      
        1 atacggaaca tggttctgtg acactgcgga tgacatggca gccgctggat ggagaaatga
       61 tcgctcttct gattgaggtc gaggataccg gggccggtat caaagaggaa gatattccaa
      121 aaatctatga aaagtttcag cgcattgata ttgagaaaaa taaaaatata gaaggtaccg
      181 gattgggaat gacaattacg gcacagtttg ttaaaatgat ggatggagag attcgtgtac
      241 gcagccagta cggaagcggc acctgcttta atctgaagat tccgcagaag atccgcagcc
      301 aggaagtgat cggcagtatt caaaaatcgc gggaaagaga gctggcggaa ttaaaaaggg
      361 atcgtgtgac ttttacagca ccaaaggcaa ggatccttgt tgtggatgac aatgagatca
      421 atctgaaagt atttgaagga ctgcttcgca aaacacagat ccatattgaa acagcagaaa
      481 gcggcgcgga gtgtctgaaa ttaacagagg aacaaaccta tgatctgatc tttatggatc
      541 atatgatgcc gcagatggac gggatcgaag cctttcggcg tcttaaggca caggacaccg
      601 ggaagtgcag ggagacgccg gtgatcgcac tgacggctaa tgcaattgtc ggtgcaaggg
      661 aagaatatat cgcagaaggt tttacggatt atttaagcaa gccaatagac ggcatcagac
      721 ttagggagat gctgctcaga tatttgccga aacagctgat cataaaaata taaggaacca
      781 ggaggaaata ttgagatgag aataggaatg ggttatgacg tgcatcgtct gactgaaaat
      841 agggaactga tacttggcgg cgtaaaaata ccatttgaaa aaggactggc cggtcattcg
      901 gatgcggatg ttgtgctgca tgcaattatg gacgctctgc tcggtgcggc ggcgctcggt
      961 gatatcggaa agcattttcc ggataccgag gaaagctata aagacatttc cagccttgtg
     1021 ctgttaaaac gtgtggggga acttttggag gaaaatcaat attttattga aaatatcgat
     1081 gcgactatta ttgcacagcg tccgaagctg cgtccgtata ttgacgaaat gaataaaaat
     1141 atagcggatg tgcttgggat tgcactcaat caggtcaata tcaaggcgac gacagaggaa
     1201 ggtcttgggt ttaccggaag cggtgaagga atttcgtcgc aggcgatctg catgctgaag
     1261 acggcagcga tcatgaaaaa taagtgagaa ggtgtggcta tgtatacaag ctttgtcggc
     1321 ggtgtcattg ctgccgcaag caaaaaagat gcggctcctc t
//
LOCUS       DAOSYE010000727         1360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89028_length_1360_cov_2.773946, whole genome shotgun sequence.
ACCESSION   DAOSYE010000727 DAOSYE010000000
VERSION     DAOSYE010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89028_length_1360_cov_2.773946"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLU43_10980"
     CDS             <1..411
                     /locus_tag="PLU43_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOO28976.1"
                     /translation="VFTELFTARPHVFISSKHPLADRESIALEELDNYPYLSFEQGNN
                     NSFYFSEEILSTLDRRKNIRVRDRATLFNLVVGLDGYTVSSGIISRELNGGNIIAKPL
                     RVKEMMRIGTITVRGVALSRYGNAYIAALKKHIR"
     gene            550..>1360
                     /gene="metF"
                     /locus_tag="PLU43_10985"
     CDS             550..>1360
                     /gene="metF"
                     /locus_tag="PLU43_10985"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOO28977.1"
                     /translation="MLREKLAKKRPILSFEIFPPKREEALTDIEGTIEVLCDLKPDFI
                     SITFGAGGTDTNSLTIEMARLIKEKYGTEPMVHLTCLNHDHEEILKILARLQEYGIEN
                     ILALRGDRREGVRPKEEFHHASDLIRFIRKHGDFMIGAACYPEGHTESKSMEEDIRYL
                     KQKVDAGAEHLLSQLFFDNRFFYSFMENAKAAGITASVEAGIMPVISRQQTEKMTALC
                     GASLPEKFTYMMRKYENNKEALSDAGIAYALDQVVDLLAHDTDGIHIYTMNQ"
BASE COUNT          415 a          262 c          349 g          334 t
ORIGIN      
        1 gtttttaccg aattgtttac ggcaaggccg catgtattca tcagttctaa gcatccgctt
       61 gcagaccggg aatccattgc acttgaggaa ctggacaatt acccgtatct ttcctttgaa
      121 caggggaata ataattcgtt ttatttttca gaggaaatat taagcacact cgaccgcaga
      181 aaaaatatca gggtcaggga ccgggcgaca ctgtttaatc tggtcgttgg tcttgacggt
      241 tatacggtca gctccggaat catcagcagg gagttaaacg gcgggaatat tattgcaaaa
      301 ccgctgcgtg taaaggagat gatgcggatc ggaacgatca ctgtcagggg cgtggcgctg
      361 agccggtatg ggaatgctta tattgccgct ctgaagaaac atatcagata atcatatgga
      421 aatttgtgta acagccgctt ggtatagttt gaaactatat caaacggctg tttttatata
      481 ttattcaata tcagaaaaat ttattacaat cataataaac cgaaaatcat agagaaaaga
      541 gggagccata tgctgcggga aaaacttgca aaaaaaagac caattctgtc gtttgaaata
      601 tttcctccaa aaagagagga ggcgttaacg gatattgaag gaacgatcga agtactgtgc
      661 gatctgaagc cggattttat cagtattaca tttggtgccg gcggaacgga tacaaacagc
      721 ctgacgatcg agatggcacg gctgatcaaa gaaaaatatg ggacagaacc aatggtgcat
      781 ctgacctgcc tgaatcatga tcatgaagag atcttaaaga tccttgcacg cctgcaggaa
      841 tatgggatcg aaaatattct ggcgcttcgc ggagacagga gagaaggtgt gcgtccgaag
      901 gaggaatttc atcatgcttc tgatctgatc agatttatca ggaaacatgg cgatttcatg
      961 atcggtgcgg cgtgttatcc ggaaggacat acggaatcaa aaagcatgga ggaggatatc
     1021 cggtatttga aacagaaagt ggatgcagga gcagagcatc tgttgtcaca gctatttttt
     1081 gataaccggt tcttttattc atttatggaa aatgcgaaag ccgccggcat cacggcatcc
     1141 gtcgaggcgg ggatcatgcc ggtcataagc agacagcaga cggagaagat gacggcgctg
     1201 tgcggggcat cccttccgga gaaattcact tacatgatgc gaaaatatga aaataacaaa
     1261 gaagcccttt cagatgccgg gatcgcatac gcccttgacc aggttgtaga cctgctggca
     1321 catgatacgg acggcattca tatttacacg atgaatcagg
//
LOCUS       DAOSYE010000728         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89454_length_1356_cov_3.534973, whole genome shotgun sequence.
ACCESSION   DAOSYE010000728 DAOSYE010000000
VERSION     DAOSYE010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89454_length_1356_cov_3.534973"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLU43_10990"
     CDS             <1..690
                     /locus_tag="PLU43_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO28978.1"
                     /translation="PYAENNDEVLYLCFSTGIAGTFGAATLAYQDLLEEYPDFKMTIV
                     DSHCASLGFGLLVYKLLLMQEQGAPDKLIKEAAVYYADNHIKHYFTVDTLYYLIRGGR
                     ISKVKGSVGEVLDVKPIITVDEDGALKCLYSVRGRKKALKKIVEESVNDCMDFSGTIV
                     GIVHGEDEESALQFEEIMREHLTPVRVIKSCVGCAIGAHTGRGIIGFIFTDRDRAEYM
                     AYQIIDKAAEY"
     gene            741..>1356
                     /locus_tag="PLU43_10995"
     CDS             741..>1356
                     /locus_tag="PLU43_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOO28979.1"
                     /translation="MVKRIYVEKKAAFAVKAKELSEEIRSYLDIRTIDNVRELIRYDI
                     ENLSEETYERACRTVFSEPPVDILYEEDFPRSKDAKVFSVEFLPGQFDQRADSAVQCV
                     KFLKEDEEPIIKTAVTYCFEGMLSDEEFERIKSYCINPVDSRETGLIKPETLVTEFEE
                     PADISIFDGFKDMDEEPFRELYESLGLAMTFNDFLHIQNYFKAEE"
BASE COUNT          392 a          236 c          376 g          352 t
ORIGIN      
        1 ccttatgcgg aaaataacga cgaggtgctg tatctttgtt tttccacggg aatcgccgga
       61 acctttgggg cggcgacgct tgcctatcag gatctgcttg aggaatatcc ggattttaaa
      121 atgacgatcg tggactctca ctgtgcttct cttggcttcg gtctgcttgt atataaactt
      181 cttctgatgc aagagcaggg tgcgcccgac aagctgatca aagaggcagc cgtatattat
      241 gcggataatc atatcaagca ttattttacg gtagatacgc tttattatct gattcggggc
      301 ggcaggatca gcaaagtgaa aggttctgtc ggtgaagtgc tggatgtgaa gccgatcatt
      361 accgttgatg aggatggagc gctgaaatgt ctgtacagtg tcaggggaag aaaaaaagca
      421 ctgaagaaga tcgtggagga gtcggtcaac gactgcatgg atttttcggg tacgattgtt
      481 ggaatcgtgc atggggaaga cgaggaatct gcgctgcagt ttgaagagat catgcgggaa
      541 catctgacgc cggtcagggt catcaaatcc tgcgtgggct gtgcgatcgg tgcccatacc
      601 ggcaggggga tcatcggttt tatatttacc gacagagaca gagcggagta tatggcctat
      661 cagataatcg ataaagcagc agagtattga tgatctggat gagaataggc aaactataaa
      721 acatgaagga gaaataaaca gtggttaaac ggatctatgt agagaagaaa gcagcttttg
      781 cggtcaaggc aaaggagctg agtgaagaaa tcagaagcta tctggatatc agaacgatcg
      841 acaatgtgag ggagctgatt cggtatgata tcgaaaatct ttcggaggaa acgtatgagc
      901 gcgcctgcag aaccgtattt tccgaaccac cggtggatat actctatgaa gaggattttc
      961 caagaagtaa agatgcaaag gttttttccg tggaatttct tccgggacag tttgatcaga
     1021 gagcggattc tgctgttcag tgtgtaaagt ttttaaaaga ggacgaggag ccgatcataa
     1081 aaacggctgt aacatactgt tttgaaggaa tgctttccga tgaagaattt gagcggatca
     1141 aaagctattg catcaatcct gttgattcca gggaaacagg cctgataaaa ccggaaacgc
     1201 ttgttacgga atttgaggaa cctgcggata tcagtatttt tgacggattt aaggatatgg
     1261 acgaagaacc attcagggaa ctgtatgagt cgcttggtct tgccatgacg ttcaatgatt
     1321 ttcttcatat tcagaattat tttaaagcgg aagaga
//
LOCUS       DAOSYE010000729         1355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89575_length_1355_cov_2.865385, whole genome shotgun sequence.
ACCESSION   DAOSYE010000729 DAOSYE010000000
VERSION     DAOSYE010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89575_length_1355_cov_2.865385"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLU43_11000"
     CDS             complement(<1..858)
                     /locus_tag="PLU43_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO28980.1"
                     /translation="MYRLVKNNIRQYTGPALAAVITLFIQTMTDLYLPALNADITNNG
                     MAKGDIPYIIRTGLIMLAIVAFGVCCMIVSSYFSSMAAMGLGKNIREQLFCHVETLSK
                     GDIDTVGTASLITRATNDVMQVQNAAMMMMRMMLMAPIMFIGGIIMAGRKSPELVWVI
                     LLAFPFIAALLIYMLKKGMPLFKSIQKKIDRVNQVVREYLSGIRVIRAFCKDDYEKER
                     FEEANLDLMQVSLKVFQMMAFMLPAMMLIVNATNIAIVWIGGKYMVAGKVQVGDLTAF
                     ITYVAMILMS"
     gene            complement(1008..>1355)
                     /locus_tag="PLU43_11005"
     CDS             complement(1008..>1355)
                     /locus_tag="PLU43_11005"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOO28981.1"
                     /translation="NECPKCHRLYTAEYMKNADGVPLCVHCKQPIRPHVKLHGELIRN
                     DKMTQAANACQAAHMILVLGTNLNHPMVKSFLRYYTGNKVILITKHEHYSDKQADLCI
                     YGEVQDILPRVIG"
BASE COUNT          383 a          334 c          244 g          394 t
ORIGIN      
        1 cgacatcagg atcatggcta cataagtaat aaaagcagtc agatcaccaa cctgtacttt
       61 tcctgcaacc atatatttcc cgccgatcca tacgatcgca atattcgttg cattcacgat
      121 cagcatcatt gccggaagca taaatgccat catctgaaat accttgagtg atacctgcat
      181 caaatcaaga ttggcttctt caaaacgttc cttttcataa tcatctttac agaatgcccg
      241 aatgacacgg atcccgctta aatattcccg tacgacctgg ttgacccggt ctattttttt
      301 ctgaatgctt ttaaacagag gcatcccttt tttcagcatg tagatcagga gggcggctat
      361 aaagggaaac gcgaggagaa tgacccatac aagctccgga ctcttcctgc ctgccataat
      421 aataccgccg atgaacatga tcggtgccat aagcatcata cgcatcatca tcatggctgc
      481 attctgtacc tgcatcacat cattggtcgc ccttgtgatc agggaagcgg tcccgacagt
      541 gtcgatatct ccttttgaca gtgtttccac atgacagaac aactgttccc ggatgttctt
      601 tccaagtccc atcgcagcca tggaagaaaa ataactgctg acaatcatac agcacacgcc
      661 gaatgccaca attgcaagca tgatcagccc tgttctgata atatagggaa tatctccttt
      721 tgccatgccg ttattcgtaa tatcggcatt caaagccggc agatacagat ccgtcattgt
      781 ctgtataaac agcgtgataa cagcagccag tgccggtccg gtatattgcc ggatattgtt
      841 ttttaccaat ctgtacatag cgtcctccca ttttaaagca tctttataat ttacacgttt
      901 catttcccaa tgtcaacaaa catttcgtaa aaaaaacttc tcattcaaaa gaatcagaag
      961 tttcttatct ataattctgt tttcaataaa tcctgcttca aaaagcatta tccgatgacc
     1021 ctggggagaa tgtcctgtac ctctccataa atacacaaat ccgcctgctt atccgaataa
     1081 tgttcatgtt ttgtaataag gatcacctta tttccagtat aatagcgcaa aaacgatttt
     1141 accattggat gattcagatt tgtgccaagg accaatatca tatgtgctgc ctgacaggca
     1201 ttggcagcct gtgtcatctt atcatttctg atcagttctc catgcagctt tacatgcggc
     1261 ctgatcggct gtttgcaatg tacacaaagc ggaacgccgt ccgcattttt catatattct
     1321 gccgtataaa gccgatgaca tttgggacat tcatt
//
LOCUS       DAOSYE010000730         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89683_length_1354_cov_3.086990, whole genome shotgun sequence.
ACCESSION   DAOSYE010000730 DAOSYE010000000
VERSION     DAOSYE010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89683_length_1354_cov_3.086990"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLU43_11010"
     CDS             <1..665
                     /locus_tag="PLU43_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein F"
                     /protein_id="HOO28982.1"
                     /translation="AVESEDEATRTDEFQRLPLRIGDSLIGFKEVQESDSIYLLLLGL
                     GAGILVYFRKDRELLNREKARNQEMLRDYPKIVNKYALYYNAGMSSKVIWQRICSDYR
                     KEYERSGIKRYVYEEMLAADESMRDGCGEIAAYEKFAARCQLPSYRLFVNLIEQALQK
                     GKENMGELLEKEAGDAFKKRKNAAKKRGEEAGTKLLLPMFLMLFVVLFIVIFPAFFSF
                     GI"
     gene            703..873
                     /locus_tag="PLU43_11015"
     CDS             703..873
                     /locus_tag="PLU43_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HOO28983.1"
                     /translation="MYKVMKRFIQEESAIGVVELILILVVLIGLVIIFRDQLTSLLNG
                     IFERIVSDSAAV"
     gene            886..>1354
                     /locus_tag="PLU43_11020"
     CDS             886..>1354
                     /locus_tag="PLU43_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28984.1"
                     /translation="MKRGEITVFFALLLSAVCVFFCAVIESARTQAMRFQIETLMDMG
                     LHSCFGEYQQELFARYDLLYIDTSYGRESGDITKTLRHLEQYIEENAGSGKAGAQTLL
                     QEKGDWFQLSIEKSEAVQFLLASDFNGHTLRNQAVHYIRVYGDSSHSAAMAKTA"
BASE COUNT          408 a          229 c          360 g          357 t
ORIGIN      
        1 aagcagtcga atcagaggat gaagcgacca ggacagatga atttcagcgg cttcccctgc
       61 ggatcggcga cagcctgatt ggttttaaag aagtacagga aagcgacagt atttatttgc
      121 tgctgcttgg gcttggagcc gggatcctgg tttattttag gaaggacaga gaattattaa
      181 acagagaaaa ggcgcgtaat caggaaatgt taagggacta tccgaagatc gtcaataagt
      241 atgcacttta ttacaatgca ggaatgtctt cgaaagtgat ctggcaaagg atctgcagtg
      301 attacagaaa agaatatgaa agatccggga taaagcgtta tgtatatgaa gaaatgctgg
      361 cagcggatga aagcatgcgg gacggctgcg gggagattgc agcgtacgaa aaatttgcgg
      421 cgcgctgtca gcttccgtcc taccgccttt ttgtcaatct gatcgagcag gctttgcaaa
      481 aaggaaagga gaatatggga gaactgcttg aaaaagaagc aggtgacgca ttcaaaaaaa
      541 gaaagaatgc tgcgaaaaag cgcggagagg aagcaggaac aaaattgctt ctgccaatgt
      601 ttttgatgtt atttgtcgta ctttttatag ttatatttcc ggcatttttc agttttggaa
      661 tttgacctgg aaatcaatga aaaattgaaa ggaagggatt ttatgtataa agttatgaaa
      721 cggtttattc aggaagaatc agcaattgga gttgtagaac tgatattgat ccttgtagtg
      781 ctgatcggtc tggtcatcat attccgtgac cagctgacat cgctgctcaa tggtattttt
      841 gagaggatcg tcagtgacag cgcggccgta taggcggaac tgtttatgaa aagaggagaa
      901 attacggtat tttttgcgct gcttttgtct gcagtctgtg tatttttctg tgctgtgatt
      961 gaaagtgcgc gtacacaggc aatgcggttt cagatagaaa cactgatgga tatgggactg
     1021 cattcctgct ttggagaata ccagcaggaa ttatttgccc gttatgatct gctgtatatt
     1081 gacacctcct atggcagaga atccggtgat ataacaaaga cactgcggca tttggagcag
     1141 tatatcgaag aaaatgcagg cagcggcaaa gccggggcgc aaactctttt acaggaaaaa
     1201 ggggactggt ttcaattatc cattgagaaa agtgaggccg tacaattcct tttagcaagt
     1261 gattttaacg ggcacacact tagaaatcag gccgttcatt atatcagggt atacggagac
     1321 agttcgcata gtgccgcaat ggcaaaaaca gctg
//
LOCUS       DAOSYE010000731         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89715_length_1354_cov_2.345651, whole genome shotgun sequence.
ACCESSION   DAOSYE010000731 DAOSYE010000000
VERSION     DAOSYE010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89715_length_1354_cov_2.345651"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PLU43_11025"
     CDS             complement(<1..1102)
                     /locus_tag="PLU43_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO28985.1"
                     /translation="MAGREYPLERTRNIGIMAHIDAGKTTLTERILYYTGVNYKIGDT
                     HEGTATMDWMEQEQERGITITSAATTCHWTLHENAKVKDGELEHRINIIDTPGHVDFT
                     VEVERSLRVLDGAVGVFCAKGGVEPQSENVWRQADTYNVPRMAFINKMDILGADFYAA
                     VEQIKTRLGKNAICLQLPIGKEDEFKGIIDLFEMQAYIYNDDKGEDISVGPIPDDMAD
                     EAELYRTELIEKVCELDDDLMMQYLEGEELSVADMKKALRKGTCECTAVPVCCGSAYR
                     NKGVQKLLDAILEFMPAPTDIPAIKGVDMEGNEIERHSSDEEPFSALAFKIMADPFVG
                     KLAFFRVYSGTMKSGSYVLNATKEKKERVGRIL"
     gene            complement(1117..>1354)
                     /locus_tag="PLU43_11030"
     CDS             complement(1117..>1354)
                     /locus_tag="PLU43_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO28986.1"
                     /translation="RIGGATYQVPIEVRPERRQALALRWITMFSRKRGEKTMEERLAN
                     EILDAANNTGAAVKRKEDMHKMAEANRAFAHYRF"
BASE COUNT          339 a          370 c          231 g          414 t
ORIGIN      
        1 gcaaaatacg tccgacacgt tccttttttt cttttgtagc attcaacacg taagaaccgg
       61 atttcattgt tccggaatac actctgaaaa acgcaagctt tcctacaaac ggatcagcca
      121 tgatcttaaa tgccaatgcc gagaacggct cttcatccga agaatgcctc tcgatctcat
      181 ttccttccat atccacaccc ttaatagctg ggatatcggt tggtgccggc ataaattcca
      241 atattgcatc cagaagtttc tgtacacctt tgtttctgta cgcagaacca cagcatacag
      301 gaactgccgt acattcacac gttccttttc tcaatgcctt cttcatatct gctacggaaa
      361 gctcttcacc ttccaaatac tgcatcatca gatcgtcatc cagttcacag actttttcaa
      421 tcaattccgt acggtaaagt tcagcttcgt ccgccatatc atccggaatc ggtccgacgg
      481 aaatatcttc acccttatcg tcattgtaaa tatacgcctg catctcgaac aaatcgataa
      541 tgcctttaaa ttcatcttcc ttaccaatcg gcagctgcag acagatcgca ttttttccta
      601 atcttgtttt gatctgttct accgccgcat aaaaatcagc tcctaaaatg tccatcttat
      661 tgataaaagc cattctaggt acattatagg tatcagcctg acgccatacg ttctctgact
      721 gcggttcaac accgcccttt gcacagaaca cacctacagc tccatcaagt acacgaaggg
      781 aacgctcaac ttccacggta aaatcgacgt gacccggcgt atcaataata ttgatacggt
      841 gctcaagctc gccgtctttt accttcgcat tttcatgaag cgtccagtgg caggtcgtag
      901 cagccgatgt aatggtaata cctctttcct gctcctgttc catccagtcc attgtagcag
      961 taccttcgtg agtatcacca attttataat tcacaccagt ataatagagg atacgctctg
     1021 tcaacgtggt cttacccgca tcgatatgag ccatgatacc aatatttctg gttctctcta
     1081 atggatattc tcttccagcc aagtttcttc ctcctattag aatctgtaat gtgcaaaagc
     1141 cctgtttgct tctgccattt tatgcatatc ttcttttctc tttactgcag cccctgtatt
     1201 gttggctgca tccaaaattt cattagcaag tctttcttcc atggtcttct cgcctctttt
     1261 gcgggaaaac atggtgatcc agcgaagcgc taacgcctgg cgtctttccg gtcttacttc
     1321 gatcggcacc tggtaagtag ctccgccaat acgt
//
LOCUS       DAOSYE010000732         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89930_length_1352_cov_2.774094, whole genome shotgun sequence.
ACCESSION   DAOSYE010000732 DAOSYE010000000
VERSION     DAOSYE010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89930_length_1352_cov_2.774094"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1352
                     /locus_tag="PLU43_11035"
     CDS             <1..>1352
                     /locus_tag="PLU43_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO28987.1"
                     /translation="LKKYDLVTDCLKLEITESAYMDQPQELMTTIQQFQKTGFKILMD
                     DFGSGFSSLNMLKDVNVDILKIDMRFMESLETSDRAGNILFSIIQMAKGIHMEVIAEG
                     VETENQYELLVSMDCDGVQGYYFYKPLMEEEIGRKLDEEALEKSLTRTAGQKSILVVD
                     DVEMERELLCRIIEGDYHVLTAASVDRAMEILKREFANIALVITDIIMPDKNGFVLLK
                     QMKKMMFFSSIPVLMMTADGEYENVEKAISMGALDVIGKPFDAPILRQRLKNILKISE
                     RETVSREIHALKENVVLRKQLDSLLEDSVAGIARVRLCHDEPYAIKEVVYVNDRFLYF
                     HQIQEPETLNRKALYQLMKNTLSGDLEALGKKFEKAVKAHEKFVGGEYHLEGDNGFYK
                     NMYVSCTIRYYQEEILLDLLVMECRTGVEYQLDRSMQTFSELLGVQTGLGAWRYYPID
                     "
BASE COUNT          400 a          232 c          360 g          360 t
ORIGIN      
        1 tacttaaaaa atatgatctt gttacggatt gcctgaagct tgaaattacg gaaagcgctt
       61 atatggatca gccgcaggag ctgatgacga cgatccagca gttccaaaag accggattta
      121 aaatattgat ggacgatttc ggaagcggtt tttcatcgct taatatgtta aaggatgtca
      181 atgttgacat attaaagatc gatatgaggt ttatggagag tctggaaacg tctgaccgtg
      241 ccggaaatat tctgttcagt atcattcaga tggcaaaggg gatccatatg gaagtcatcg
      301 ccgaaggcgt agagactgaa aatcagtacg agctgcttgt cagtatggat tgtgacggtg
      361 tacagggcta ttatttttat aagccgctta tggaagaaga gatcggaagg aagcttgatg
      421 aggaagcgct tgagaagtct ctgacaagga ctgccggtca gaaatccatt cttgtggtag
      481 atgatgtcga aatggaaaga gaactgctct gccgcattat cgaaggcgat taccatgtgc
      541 tgactgcggc ttccgttgat cgtgccatgg aaatactgaa gcgggaattt gccaatattg
      601 cgcttgttat tacagatatt atcatgcctg acaaaaatgg attcgtgctg cttaagcaga
      661 tgaagaaaat gatgtttttc agcagtattc ctgtattgat gatgacggcg gacggagaat
      721 atgagaatgt tgaaaaagca atttcaatgg gagccctgga tgtgatcggc aaaccttttg
      781 atgcaccgat cctgcgtcag cgtcttaaaa atattctgaa aatatcggaa agggagaccg
      841 taagccgtga gatccatgcg ctcaaggaaa atgttgtatt gaggaaacag ctggattcgc
      901 ttttggaaga cagtgttgcc ggaatcgcaa gagtacggct ctgccatgat gaaccgtatg
      961 caataaagga agttgtttat gtcaatgacc gttttttgta tttccatcag attcaggagc
     1021 cggagacact gaaccggaaa gcgctgtatc agcttatgaa aaacacgtta tccggggatc
     1081 tggaggcgct tggcaaaaaa tttgagaaag cagtcaaagc acacgaaaaa tttgtcggcg
     1141 gggaatatca tcttgaagga gacaacggtt tttataaaaa tatgtatgtc agctgtacga
     1201 tccggtatta tcaggaagaa atacttctgg atctgcttgt tatggaatgc aggacaggag
     1261 tggaatatca gctggacaga tccatgcaga cgttttcgga gcttcttggc gtgcagaccg
     1321 gtctcggtgc gtggagatat tatccgatag at
//
LOCUS       DAOSYE010000733         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89950_length_1352_cov_2.485736, whole genome shotgun sequence.
ACCESSION   DAOSYE010000733 DAOSYE010000000
VERSION     DAOSYE010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89950_length_1352_cov_2.485736"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLU43_11040"
     CDS             complement(<1..881)
                     /locus_tag="PLU43_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOO28988.1"
                     /translation="MKTQKSLKYKLISLLLAAAVGVQMFSYSVPAETTSEKIKKAEEL
                     KQATEEQKKAVEDKKETLEDTKDQLQDYLSQLNSDLTEISDNLADIEQQITDKEAEIE
                     QTEEDVEEAKDEEERQYELMKKRVKFMYEKGDTSLLEAFLTASSYSEFLNKAEYVNKV
                     EEYDQKIFENLILLRESVEEKEAALEEEKEELLTLQDQAETEHSKVNTLVNSTSGTIA
                     ATNGEITAAELEEAAYEAELKAQEENLTALKKQLAEEQAMSAKAAQMAWRDISELTFE
                     ASDRDLLACLIYCEAGG"
     gene            complement(920..>1352)
                     /locus_tag="PLU43_11045"
     CDS             complement(920..>1352)
                     /locus_tag="PLU43_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOO28989.1"
                     /translation="AMLSGMRSKDPSTQVGACIVSEDNKILSMGYNGFPTGCSDDEFP
                     WCREGEPLNNKYFYSTHSELNAILNYRGGSLEGAKIYVSLFPCNECAKAIIQSGIKTI
                     VYDCDKYADTPSVIASKRMLDAAGVRYYRYKHTNRKIEFSL"
BASE COUNT          313 a          363 c          237 g          439 t
ORIGIN      
        1 tctccgccgg cctcacagta gatcaggcat gccagaagat ctctgtcact ggcttcaaat
       61 gtcagctctg aaatatcacg ccatgccatt tgcgccgctt tcgcgctcat tgcctgttct
      121 tcggcaagct gcttcttcaa cgcagtcagg ttctcctcct gtgctttcaa ttccgcctca
      181 taagccgcct cttctaattc cgccgcagta atttccccat ttgtcgccgc gattgtccca
      241 gaagtggaat taacaagtgt attgactttg ctgtgttccg tttccgcctg atcctgtaat
      301 gttaaaagct cctccttctc ttcctcaagt gcagcttcct tttcttcaac actttccctg
      361 agcaaaatca gattttcgaa tattttctgg tcatattctt ctaccttatt gacatattct
      421 gccttattta aaaattcgga atagcttgac gcggtcaaaa aagcctccag taaagaagtg
      481 tcgccttttt catacatgaa cttcactctt tttttcatga gttcatattg cctttcttct
      541 tcatccttcg cttcttcaac atcctcttcc gtctgttcga tctccgcttc cttatcggtg
      601 atctgctgct cgatatccgc aagattatca ctgatctcgg tcagatccga attcagctgt
      661 gaaagataat cctgcagctg atcctttgta tcctccaatg tttctttttt atcttctacc
      721 gcttttttct gctcctccgt ggcctgtttc aattcctcag ccttcttgat tttctcggaa
      781 gtcgtttccg caggcacaga ataagaaaac atctgcacac caactgcagc tgccaataac
      841 agactgatca atttatattt cagagatttc tgtgttttca tagtctgcgg atatctccca
      901 atcaattatt tattccggct cacagagaaa attcaatctt tctgttcgta tgcttatagc
      961 gataataacg gacacctgca gcatccagca tacgcttcga cgcaattacg gaaggggtgt
     1021 ccgcatattt gtcacagtca tacacgatcg tcttgatccc tgactgaata atggcttttg
     1081 cacactcatt acacggaaaa agcgatacat agatcttcgc gccttcaaga ctgcctccgc
     1141 ggtaattcag gatcgcgttc agttcactgt gtgtggagta aaaatattta ttatttaatg
     1201 gttccccttc tctgcaccat ggaaattcat catccgaaca gcccgttgga aaaccattat
     1261 aacccattga taaaatctta ttgtcttcac tgacaatgca ggcaccgacc tgcgtactgg
     1321 ggtctttgga acgcatgccg gaaagcatcg cc
//
LOCUS       DAOSYE010000734         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_89971_length_1352_cov_2.097147, whole genome shotgun sequence.
ACCESSION   DAOSYE010000734 DAOSYE010000000
VERSION     DAOSYE010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89971_length_1352_cov_2.097147"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLU43_11050"
     CDS             <1..181
                     /locus_tag="PLU43_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HOO28990.1"
                     /translation="EQMDALPSGYRTILSLYLFEGYDFEEIAEITGLQPVSVRSQYSR
                     GRIKLQSAINKVWTN"
     gene            169..672
                     /locus_tag="PLU43_11055"
     CDS             169..672
                     /locus_tag="PLU43_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO28991.1"
                     /translation="MDKLKSFIQDNKTLFETESLSQEHEKRFMERLAKSQSIKKFRTY
                     KYLIYISSAALLIVVLAIGLRFLKEDPLAVFPSDPCRDGSHICYYNQIRKLSSRIEYD
                     SRNLPEYKRQEIIMNIQSLVPSSAHEFSDMLPADISDKEADRLNIEYYKQLYEGMKEI
                     ASLTNQT"
     gene            678..>1352
                     /locus_tag="PLU43_11060"
     CDS             678..>1352
                     /locus_tag="PLU43_11060"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOO28992.1"
                     /translation="MKRIALITCALVSCTFLFGQNTVKKDYRLESFNSIDASFIYEIE
                     VFKGRDHTVKLEVPEELVDHIQVYVSKNTLYLGVTKEWWRTAKHVLGDRQKIKAAVIM
                     NDLEGFQLSNSASLVTKEGFSPANFMAKLTGASKAEISIITHAANLDISGASELEISG
                     LATKTKAYVSGASIMRYNQETDQIELKASGASTVVMNGSSTSASLTVSGASHLKALDF
                     ETKETDI"
BASE COUNT          444 a          275 c          298 g          335 t
ORIGIN      
        1 ggaacaaatg gatgcccttc catcgggtta ccgcaccatt ctgagcttat atctttttga
       61 aggatatgat tttgaagaaa ttgcagaaat aacaggactg cagcctgttt cagtaagaag
      121 ccaatacagc cgtggcagga taaaactaca gtcagcaata aacaaggtat ggacaaatta
      181 aagtctttca ttcaggataa caagacactt ttcgaaacgg aaagtctttc gcaggaacat
      241 gaaaaaaggt tcatggaacg cctggcaaaa agccaaagca ttaaaaagtt ccgaacttat
      301 aagtatttaa tttacatatc ttctgcagca ttacttatag tagtgcttgc aataggatta
      361 cgcttcctga aagaggatcc gctggctgtt tttccttccg acccgtgcag agacggatcc
      421 cacatctgtt attacaacca gatccggaag ctttccagtc ggattgagta cgactccagg
      481 aacctgcctg aatataaacg gcaggagata atcatgaata tacaatccct ggtgccatca
      541 tctgcccatg aattttcgga tatgcttcca gcagacatat ctgataaaga ggcagaccgc
      601 ttgaacatag agtattataa acagttatat gaaggaatga aagagattgc ctcattgaca
      661 aatcaaacgt aaaaaacatg aaaagaattg cattaatcac atgtgcactg gtatcgtgca
      721 ctttcctttt tggacaaaac actgttaaaa aagattaccg gcttgaaagt tttaattcca
      781 ttgacgcttc tttcatttat gagattgaag ttttcaaggg cagagaccac acggtgaaac
      841 tggaagtacc cgaagaactg gtagatcaca tccaggtata tgtttcaaag aacacacttt
      901 acctgggagt gacgaaagag tggtggcgta ccgcaaaaca cgtcctgggc gacaggcaga
      961 aaatcaaagc cgctgtcata atgaacgatc tggaaggatt ccagttgtca aattcagcaa
     1021 gtcttgttac gaaggaggga ttttcgcctg ccaatttcat ggcaaaattg acaggtgcat
     1081 caaaagctga aatatctatc ataacacatg ctgccaacct tgatataagc ggggcatctg
     1141 aactagagat cagtggtttg gcaacaaaaa caaaagccta tgtcagcggt gcatccatca
     1201 tgaggtacaa ccaggagact gatcagatcg aattgaaggc gtcaggcgcc tctacggttg
     1261 tgatgaatgg aagttcaaca agcgccagtc tgacggtttc gggcgcaagc catctgaaag
     1321 cccttgactt tgaaacgaaa gagacggata tt
//
LOCUS       DAOSYE010000735         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90030_length_1351_cov_3.296296, whole genome shotgun sequence.
ACCESSION   DAOSYE010000735 DAOSYE010000000
VERSION     DAOSYE010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90030_length_1351_cov_3.296296"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLU43_11065"
     CDS             complement(<1..502)
                     /locus_tag="PLU43_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOO28993.1"
                     /translation="MQYLLEYSDTFTTPYEAFFQDTGSCTFPVKPHWHYFTEIIYIME
                     GSTMVDCSDASYILETGDLFLFPPRSIHSIYSTKWAALRYAVIKFDANALNVTNSYTP
                     KLQSVLDSVRNNPGANLCFPAADIKNLPIESLFTDCIRELSDKQYGYDIQVHIVYRKV
                     REYSPNF"
     gene            656..>1351
                     /locus_tag="PLU43_11070"
     CDS             656..>1351
                     /locus_tag="PLU43_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-xylanase"
                     /protein_id="HOO28994.1"
                     /translation="MDFVKGMTFGAFASRGSLKRKDAKSSLDNLKLRTGSDTIVLVPN
                     GLQENAHTETIFYDTKATLSDDELTEFISYVHEIGMKVFLKPTVNCMDGTWRAHINFF
                     DKEVHCEPKWRNWFASYTAFQMHFAKLAEKTGCEMFIAGCEMVQTERRSDEWRALISD
                     LREVYDGPISYNTDKYQEDNVSWWDAVDVISSSGYYPLNDWKKELDRIEMVVRKFGKP
                     FFFAEAGCMSTKGS"
BASE COUNT          410 a          230 c          350 g          361 t
ORIGIN      
        1 gaaagttcgg actgtattcg cgaactttcc gataaacaat atggacctga atatcatatc
       61 catattgttt atcggaaagt tcgcgaatac agtccgtgaa cagtgattcg atcggaaggt
      121 ttttaatatc cgcggccgga aaacagagat ttgcgcctgg attattccgg acactgtcca
      181 gaacggactg aagctttggc gtatagctgt ttgtaacatt cagggcattg gcatcgaatt
      241 ttatgacagc atagcgcagt gcggcccatt tagtggaata gatggaatga atgctgcgag
      301 gcggaaatag aaacaggtct ccggtttcca atatataaga ggcgtcgctg cagtcgacca
      361 ttgtggagcc ttccataata taaatgattt ccgtgaaata atgccaatgt ggtttgacag
      421 gaaaggtaca gctgccggta tcctgaaaaa atgcttcata cggggtcgtg aatgtatcac
      481 tgtattcaag cagatattgc atgggtttgc ctccagaata aatgttaaga gatttttaca
      541 attttttcgt acgatcatta tagcatgaaa aaaaggagga tgatatgata aagaacagaa
      601 aacagtaaat tccttataat ataggtatat aggttccaga accggaggta aaaccatgga
      661 ttttgtaaaa gggatgacat ttggtgcttt tgcatcccgc ggcagcctga aaaggaaaga
      721 tgcaaagagc agccttgata atctgaaact gcggacaggt tcggatacga tcgtgctcgt
      781 gccaaacgga ttgcaggaaa atgcgcatac ggagacgatt ttttatgata caaaagcaac
      841 attgtcggat gatgagctga cagagtttat ttcttatgtg catgagatcg gaatgaaggt
      901 atttttaaag ccgaccgtaa actgtatgga cggaacgtgg cgtgcgcata ttaatttttt
      961 tgataaagag gtgcattgtg agccgaaatg gagaaactgg tttgcgtcct atacggcatt
     1021 tcagatgcat tttgcaaagc ttgcggaaaa aaccggctgc gaaatgttta tagccggctg
     1081 tgaaatggtg cagacagaaa gacgcagtga cgaatggaga gcattgattt ctgatctgcg
     1141 ggaggtgtat gacggaccga tctcctataa cacggacaag tatcaggagg ataacgtttc
     1201 ctggtgggat gcggtggatg ttatttcttc aagcgggtat tatccgctca atgattggaa
     1261 aaaagaactt gaccgaatag agatggttgt gagaaaattt ggaaagccat tcttttttgc
     1321 ggaagcaggc tgcatgtcaa caaaaggctc c
//
LOCUS       DAOSYE010000736         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90038_length_1351_cov_3.084105, whole genome shotgun sequence.
ACCESSION   DAOSYE010000736 DAOSYE010000000
VERSION     DAOSYE010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90038_length_1351_cov_3.084105"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1351
                     /locus_tag="PLU43_11075"
     CDS             145..>1351
                     /locus_tag="PLU43_11075"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO28995.1"
                     /translation="MKKGSSCLTVKAVFIFMTSMLLLAVCANSVWAYDNVTIVIDPGH
                     GGPGTEDASDLGAQYHNVLEKDLALITAMSMKIELEQYGNVTVYLTRAEDKQMSLKER
                     VDYAAALGADVIVSLHYNASTYHRFYGAEIFTSAFGQNYATGYGLAQCVMKQWTANGA
                     VDKGIKTRLGDGGEDYYGLIRRGTEVQIPTIILEHGYIDNDIDWDRIGDAAAWQYLGQ
                     LDADGIADYFGLLKSVVQVSVSPSVSVEVPLVTVYPDTTPPSDVKVTIDQYDTSTNQV
                     SFTVYAKEQESSLMYFGAATEQEAEDEDSAFMDLDLWDSGESSMSGTYTVPAGYEGSL
                     IIRVYNNYELYADSEPAVLPSETTESVQTEPVSSDTISDEQESTVSEDEAGAAGEAGA
                     VKHDHFRHET"
BASE COUNT          392 a          237 c          389 g          333 t
ORIGIN      
        1 atgtaagcag gaacaggatc agaaatttgg atggagactg catgcgggaa tgtacgaaat
       61 attttgtaat tcccctgatt atttgttata ctgataattg cagcgaagag gagcagaagg
      121 cgtgtgtaaa ggggagaagc tgacatgaag aaagggtctt cctgcctgac agtaaaagcg
      181 gtattcattt ttatgaccag tatgctgtta ttggccgtat gtgcaaactc tgtgtgggca
      241 tatgacaatg tgacgatcgt cattgatccg ggacatggcg gacccggcac agaagatgcg
      301 tcggatcttg gagcacagta tcataatgta ctggagaaag atcttgcatt aattacagct
      361 atgtccatga aaatagaatt ggagcagtat ggcaatgtga ccgtatattt gacgcgggca
      421 gaagataagc agatgagttt gaaggaacgc gttgattatg cggcggcact tggagcggat
      481 gtgatagtca gccttcatta taatgcttcg acgtatcacc ggttttatgg agcggagatt
      541 tttacctcag cgtttgggca gaactatgcg acaggttatg gacttgcaca atgtgtgatg
      601 aagcagtgga cagccaacgg agcagtggac aaaggcatta aaacaagact gggagatggc
      661 ggagaagatt attacggatt gattcgaaga ggaacggaag tccaaatacc gacaattata
      721 ctggaacacg gatatattga caatgacata gactgggacc ggataggtga tgcggctgca
      781 tggcagtatt taggtcagct ggatgcggac gggatcgctg attattttgg gctgctaaag
      841 agtgtcgtac aggtttctgt ttctccgtca gtatctgtag aggttccttt agtaaccgta
      901 tatccggata cgacaccgcc atctgatgtg aaagtgacga tcgatcagta tgatacatca
      961 acaaatcagg tttcctttac cgtttatgct aaggaacagg aaagcagtct gatgtatttt
     1021 ggggctgcta cagaacagga agcggaagat gaagattcgg catttatgga tctggacctg
     1081 tgggacagcg gagaatcgag tatgtcgggt acttatacgg tgccggcagg ctatgaagga
     1141 agtctgatca tacgggtata taataattat gaactctatg cggacagtga accggccgtg
     1201 ctgccatcgg agacaactga gagtgttcaa actgaacccg tgtcatcaga tacgatatcg
     1261 gatgagcagg aaagtactgt cagtgaagat gaggcaggag ctgctggaga agcaggagcc
     1321 gtgaaacacg accatttccg tcacgaaacg g
//
LOCUS       DAOSYE010000737         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90044_length_1351_cov_2.967593, whole genome shotgun sequence.
ACCESSION   DAOSYE010000737 DAOSYE010000000
VERSION     DAOSYE010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90044_length_1351_cov_2.967593"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLU43_11080"
     CDS             complement(<1..127)
                     /locus_tag="PLU43_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOO28996.1"
                     /translation="MKLNKNDLLLYAVTDRSWLGGFTLYEKVEEALLGGATFIQLR"
     gene            complement(114..935)
                     /gene="thiM"
                     /locus_tag="PLU43_11085"
     CDS             complement(114..935)
                     /gene="thiM"
                     /locus_tag="PLU43_11085"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOO28997.1"
                     /translation="MLETCIEKVRAAAPLVHSITNYVTVNDVANILLACGGSPIMSDE
                     PDDVADITSICGGLNINIGTLNKRSIEAMMIAGHRANELGHIVLLDPVGAGASALRTN
                     TALDLMKHLRFDAIRGNISEIKTLAYGSGTTKGVDADLADAVTESNLDGMIRFVKNFA
                     AKTNSIIAVTGSIDLVADSRKCFVIRNGRPEMGKITGTGCQLSGMMAAFMTANPERKL
                     EAAAAAVCAMGLAGEIGYKKLLDGEGNSTYRNRIIDAVYCMDTDTLAKGAHYEIK"
     regulatory      complement(1042..1150)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          334 a          322 c          312 g          383 t
ORIGIN      
        1 cacgcagctg aatgaatgtg gcgccgccaa gcagggcttc ttcaactttt tcgtacaatg
       61 taaagccgcc aagccagctg cggtctgtaa cagcgtataa cagcagatcg tttttattta
      121 atttcataat gtgctccttt tgccagtgta tcggtatcca tgcaatagac ggcgtcgatg
      181 atacggttcc ggtatgtgga atttccttcg ccatccagca gttttttata gccgatctct
      241 ccggccaggc ccattgcgca tacggccgca gccgctgcct ccagctttcg ttccggattt
      301 gccgtcataa aagctgccat catgccggag agctggcagc cggttccagt gatctttccc
      361 atttcaggcc tgccgttacg gatcacaaag catttcctgc tgtctgctac aaggtcgata
      421 gagccggtga ctgcaatgat gctgttagtt ttggccgcga aattttttac gaaccgaatc
      481 atgccgtcaa gattactttc tgtgactgcg tctgccagat cggcatccac accttttgtc
      541 gtaccgcttc cgtaagccag cgttttgatc tcggatatat tgcctcggat ggcgtcgaac
      601 cggagatgct tcatcagatc cagcgcggta tttgtacgca gtgcacttgc gccggcgccg
      661 acagggtcga gcaggacgat gtgtccgagt tcgttggctc tatgtccggc aatcatcatc
      721 gcctcgatac tgcgtttgtt cagagtgccg atgttgatat tcagaccgcc gcagatcgat
      781 gtaatatctg caacatcgtc cggttcatcc gacatgatcg ggcttccgcc gcaggctaac
      841 aggatgttgg caacatcatt taccgtaacg taattcgtaa tgctgtgtac gagcggagca
      901 gcggctctga ctttttcaat acaggtttct aacatttttt gtcctccata atataaaaga
      961 ttgatgattg agtgataaaa agcaatggaa ggaaaaatac aaaaaaaaca gatgtgcctg
     1021 ttttgttttg gcatatctgc ttactgtttt catatgatgt ccctacgttg gcattatcca
     1081 aatcaggtaa tgggtcgaag gttacgcctt cctctcagcc tgcaatacac aagctccccg
     1141 ctgtttgcta ttcaattatc gctttcatta tatgcgcatt cctataaaaa tgcaagttaa
     1201 tactacaaga gattggcaac agtatgatat aatacgaaaa aaggaaaaat caggctgcgc
     1261 ttcaaaccgg aggaagaaac ttgtttcaat atattttgaa taagtacagg gaatatggga
     1321 ttgccaaaaa gctgctgatc atgctgcttt g
//
LOCUS       DAOSYE010000738         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90225_length_1349_cov_3.533230, whole genome shotgun sequence.
ACCESSION   DAOSYE010000738 DAOSYE010000000
VERSION     DAOSYE010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90225_length_1349_cov_3.533230"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1349)
                     /locus_tag="PLU43_11090"
     CDS             complement(174..>1349)
                     /locus_tag="PLU43_11090"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOO28998.1"
                     /translation="EFAIISIDIDKFRLINDRYGVEIGNRSLMILGKTIKKVAQKNSI
                     AKRYQGDVFSVLLSYKTDQDILNYMMTLSDRMHDNDLLPTSISLVYGIYKVTDREVPI
                     RLMCDRARIAKKQIKGSTLTNYAVYDDVIRLKMREQVEIEDEMEKALLNHEFVMYLQP
                     QIWVEKRKICSAEALVRWKHPIKGVLVPAQFLQLFESNGFITKLDTYMWEEACKYLAD
                     LQKRKIDLPISVNISRVHIGNTDLPDILSGLVEKYKISPRLLELEITENLFMDDVTEL
                     FDQMAELKKRGFKILMDDFGSGYSSLNMLRKAPVDTLKIDRFFLDEILSTERGKIIVE
                     SSVRMAKLLGLLVVAEGVETKEQLDFLASVNCDIAQGFYFSKPIPLAEFEVFLSKNI"
BASE COUNT          378 a          344 c          224 g          403 t
ORIGIN      
        1 caggccgaaa ttttttacgg attcaatacc ctgtcctgtc aacttggaat ttctgtcata
       61 aagtaatacg ccttttttat tgtaaatcgg tttagccagc cgcatacctg tttttaaatc
      121 ttccgtttta atgaataaca taaatactcc ctcccacagt cagcttgttc catttagata
      181 tttttactta aaaagacttc aaattccgca aggggaatcg gttttgaaaa ataaaatccc
      241 tgtgcaatat cgcaattcac gctggccaga aaatccagct gttcctttgt ttccactccc
      301 tccgcaacaa caagcagtcc gagcagcttt gccattctca cagaggattc cacgatgatc
      361 ttcccgcgtt cggtagaaag aatttcatcc agaaagaacc gatcgatctt caatgtgtca
      421 accggcgcct tcctcagcat attgagtgaa gaatacccgg aaccaaaatc atccatcaaa
      481 atcttaaatc cccgtttttt taattccgcc atctgatcaa acagctccgt aacatcatcc
      541 ataaacagat tttccgtaat ctcaagctca agcagccgcg gggatatttt atacttttcc
      601 acaagccctg acaaaatatc cggcagatcc gtatttccta tatgtacacg ggaaatattg
      661 accgatatcg gaagatcaat tttacgtttc tgcagatcgg ccagatactt gcacgcttcc
      721 tcccacatat aggtatcaag cttcgtaata aatccattgc tttcaaacag ctgcagaaac
      781 tgtgccggaa caagcacacc cttgatcgga tgcttccacc tgacaagcgc ttccgcactg
      841 cagatcttac gtttttccac ccagatctga ggctgcagat acattacgaa ttcatgattc
      901 agaagcgctt tttccatttc atcttctatt tctacctgtt cacgcatctt aagacggatc
      961 acgtcatcat ataccgcgta gttcgtcaat gtactccctt tgatctgctt cttggcgatc
     1021 cttgcgcgat cacacatcag acggatcggt acttctctgt ccgtcacctt gtaaatacca
     1081 tatacaagag aaatgcttgt cggcagcagg tcattatcat gcattctgtc agaaagcgtc
     1141 atcatataat ttaaaatatc ctggtcggtc ttataactta ataatacact gaacacatct
     1201 ccctgataac gtttggcaat agaatttttc tgtgcgactt ttttgatcgt cttaccaagg
     1261 atcatcaggc tccggttacc gatttcgacc ccataccggt cattgatcag ccggaattta
     1321 tcaatatcga ttgaaataat agcaaattc
//
LOCUS       DAOSYE010000739         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90246_length_1349_cov_2.935858, whole genome shotgun sequence.
ACCESSION   DAOSYE010000739 DAOSYE010000000
VERSION     DAOSYE010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90246_length_1349_cov_2.935858"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..707
                     /locus_tag="PLU43_11095"
     CDS             132..707
                     /locus_tag="PLU43_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO28999.1"
                     /translation="MVNIIESLKERRSYYNIGKELPVEENKVLELVEELTELVPDAFN
                     MKSSRIVVVLGEKQDLLWDTIYDVFEGKVAREKIDSFRSGAGTILYFYDTKTVKGLQE
                     KFPSYAANFPTWAQQSSAMLQISIWSGLRELGIGASLQHYNPVIDGKVRELFDLPASY
                     FLVAQMPFGGIKQEPDAKEREDISKRVKIEK"
     gene            complement(719..>1349)
                     /locus_tag="PLU43_11100"
     CDS             complement(719..>1349)
                     /locus_tag="PLU43_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO29000.1"
                     /translation="GILYTTVGKTGFITSLYIIFVPIFGLFLHKRIHWYVWFGIIIAL
                     TGMYLLCMNEKAALGIGDMLILLCAATTAVHILLTDHFSENVNSILFSCIQFLVCSIL
                     SLFAALLFEKITPDAITAASLPIIYTGVFSCGIAYTLQTLGQKYVSPVTAALILSMEA
                     VFSVIAGWVFLGETLTLKELLGCIFIFTSILSAQLPEMLKTRRQNRRRA"
BASE COUNT          424 a          248 c          344 g          333 t
ORIGIN      
        1 tagccgcagt atatccccgg aatgaagcaa ggatcatgga gatcatgaaa ttttggacag
       61 aggaagagca ggtctgtttg gctgatctgt tgaaaagatt cggaaatgaa aacaataaag
      121 gaggtcatga tatggtaaat attatagaat cattaaaaga gcgaagaagt tactacaata
      181 tcggcaaaga acttcccgtt gaggaaaaca aggttttgga actggtggag gaactgacgg
      241 aactggtgcc tgatgcgttt aatatgaaaa gttcccggat cgtagttgtc cttggagaaa
      301 agcaggatct gctgtgggat acgatttatg atgtatttga aggaaaggtt gcccgggaaa
      361 agatcgacag cttccgcagc ggtgccggaa cgatcctgta tttttatgat acaaagaccg
      421 tgaaaggtct gcaggaaaaa tttccgtcgt atgcggcaaa ttttccgaca tgggcacagc
      481 agtccagtgc catgcttcag atcagtatat ggagcggact gcgtgagctt ggaatcggag
      541 cttctctgca gcattataat ccggtgatcg acggaaaggt cagggaattg tttgatcttc
      601 cggcaagtta ttttcttgtt gcgcaaatgc cttttggcgg gattaaacaa gagccggatg
      661 ccaaagaaag ggaagatatt tccaagcggg tcaaaatcga aaagtaagtg taacaaatct
      721 atgcccgccg gcgattttgt cggcgggttt ttagcatttc cggaagctgg gccgacagga
      781 tcgatgtaaa gataaaaatg cagcccagga gttccttaag tgtaagggtt tcgcctaaaa
      841 agacccatcc ggcaatgacg gaaaaaactg cttccatact tagtatcagt gccgctgtga
      901 caggagaaac atatttctgt ccgagagttt gcagtgtata ggcaataccg catgaaaaga
      961 cgccggtata tataatggga agacttgctg ccgtaatagc atccggtgtg attttttcga
     1021 ataacagggc ggcaaaaagg ctgagtatac tacacaccag aaactgaatg caggaaaata
     1081 aaatactgtt tacattttct gaaaaatgat ctgtcagcaa tatgtgcact gcggttgtgg
     1141 ccgcacagag aagaataagc atatcaccga tcccgagtgc tgctttttcg ttcatacaaa
     1201 gcaagtacat tcctgtcagc gcaattatga tgccgaacca gacataccag tgaatccttt
     1261 tatggagaaa cagaccaaag atcggtacaa aaatgatata taaggaagta ataaaaccgg
     1321 tttttccgac ggtcgtgtat aaaataccg
//
LOCUS       DAOSYE010000740         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90267_length_1349_cov_2.602782, whole genome shotgun sequence.
ACCESSION   DAOSYE010000740 DAOSYE010000000
VERSION     DAOSYE010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90267_length_1349_cov_2.602782"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLU43_11105"
     CDS             complement(<1..1000)
                     /locus_tag="PLU43_11105"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29001.1"
                     /translation="MYKVIFADDEILIREAVSENTPWSEAGFKLTGTAENGRDAIALM
                     EKDCPDLLLTDIRMPIMDGLELSSYVQKHFPDTKVMILSGHDEFEYAKRALKYGVSEY
                     ILKPITSQELCEELKKIKEKLDALKCERENVRKIQRAYQNNLPMLRDLYLNRVIEGNS
                     SRNDLPEQLTHFNVKLYGGYQAVCMAVKEDSSEFFSKYKDMNRDIIEFAIYNIAHEIM
                     EDYEQANCFRNMNDQTVFICTADTEKKLQEMIEKIGESIIFEMNKCMKTKVSILVGTT
                     VKQPYEWQVSYENAKLAEEFRFLLEDYEFVYGRDFIMKAERGGIKTSLWNERLVLLI"
     gene            complement(1119..>1349)
                     /locus_tag="PLU43_11110"
     CDS             complement(1119..>1349)
                     /locus_tag="PLU43_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HOO29002.1"
                     /translation="LLCRMTANACHTKVSAGPVEATVYGNIVLQLIAAGAVGSIKEAR
                     EMIAKSPDISVYEPCDESEWEEAYMRYRKIVG"
BASE COUNT          350 a          365 c          218 g          416 t
ORIGIN      
        1 taatcaggag cacaagacgt tcgttccaca aagacgtctt gatccctccg cgctccgctt
       61 tcataataaa atccctgcca taaacgaact cataatcctc cagcagaaaa cggaactcct
      121 cggccagttt tgcattctca taagaaacct gccattcata tggctgtttt accgttgtcc
      181 cgaccaatat gctgaccttt gtcttcatgc acttattcat ttcaaaaata atgctttccc
      241 caattttttc gatcatctcc tgcagttttt tctcagtatc cgccgtacag ataaagaccg
      301 tctgatcgtt catatttctg aaacaattgg cctgctcata gtcttccatg atctcatgtg
      361 cgatattata aatagcaaat tcaataatat cccggttcat atctttatat ttcgaaaaaa
      421 actccgacga atcctctttt acagccatgc atactgcctg atatccgccg taaagcttta
      481 cattaaaatg tgtgagctgt tccggcagat cgtttctgct gctgtttcct tcgatcaccc
      541 tgttcagata cagatcccta agcatcggca gattattctg atatgcgcgc tgtatttttc
      601 tcacattttc acgctcacac ttgagtgcat caagcttttc ctttattttt ttcagctcct
      661 cacacagctc ctgtgatgtg atcggtttta atatatattc cgatacccca tatttcaaag
      721 cgcgctttgc atattcgaat tcgtcatgac cgcttaagat catgaccttt gtatccggaa
      781 agtgcttttg tacataagaa gaaagctcaa gcccgtccat aataggcatg cggatgtccg
      841 tcaaaagcag atccggacag tctttttcca tcagagcaat cgcgtccctt ccgttttccg
      901 cagtaccggt cagtttaaat ccagcttcgc tccacggtgt attttcagaa acagcctctc
      961 tgatcagtat ttcatcatct gcaaaaatca ctttatacat atggctttcc cccaaattcc
     1021 atcaatcaag tccaaactga ttactattat agtatccgaa aacagagtgt cagtcaaaca
     1081 aataccgaca ctctgtttat agaacctgaa aacacctgtt acccgacaat cttccgatat
     1141 ctcatatacg cttcttccca ttccgattca tcgcatggtt catagacaga aatatccggc
     1201 gactttgcga tcatctccct tgcttctttg atactgccta ccgctcccgc cgctatcagc
     1261 tgcaggacga tatttccgta tacggtcgcc tcgaccggac ctgccgatac cttcgtatga
     1321 caggcatttg ccgtcatgcg gcacaataa
//
LOCUS       DAOSYE010000741         1346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90567_length_1346_cov_3.567002, whole genome shotgun sequence.
ACCESSION   DAOSYE010000741 DAOSYE010000000
VERSION     DAOSYE010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90567_length_1346_cov_3.567002"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /gene="purD"
                     /locus_tag="PLU43_11115"
     CDS             <1..1169
                     /gene="purD"
                     /locus_tag="PLU43_11115"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO29003.1"
                     /translation="GIAEVAECVDIGVMEFDRIADFAVEKSVDLVIVGPDDPLVGGIV
                     DVLETRGLRVFGPEKKAAVLEGSKAFSKDLMKKYQIPTAAYENFDSPKEALAYLKTAK
                     LPIVLKADGLALGKGVLICNTLLEAEDGVRTLMLDKKFGSAGNQIVIEEYMTGREVSV
                     LSYVDGKTIRTMTSAQDHKRALDGDQGLNTGGMGTFSPSPFYTEQVDAFCKEHIYQKT
                     VDAMAAEGRAFKGIIFFGLMLTKDGPKVLEYNARFGDPEAQVVLPRMQNDMIEVMEAC
                     IDGRLDEVDLEFADNAAVCVVLASEGYPVHYEKGYPITGLDRFKEEKGYFAFHAGTKS
                     ENGRIVTNGGRVLGITAVGEDLTKARKNAYEAVQWVSFEKQYCRSDIGKAIEEA"
BASE COUNT          380 a          225 c          411 g          330 t
ORIGIN      
        1 cgggaattgc cgaagttgca gaatgtgtgg atatcggtgt gatggagttt gacaggatcg
       61 cagattttgc agtggaaaaa tcagtcgatc tggtgatcgt tggtccggac gatccgcttg
      121 ttggcggtat tgtggatgta ctggagacaa ggggtctgcg tgtgttcggt ccggagaaaa
      181 aggctgctgt tttagaagga agcaaagcat tttcaaagga cctgatgaag aaatatcaga
      241 ttccgacagc tgcttatgag aattttgatt cgccaaagga ggcgcttgca tatttaaaga
      301 cagcaaagct gccgatcgta ttaaaggccg acggactggc cctcggcaaa ggcgttttga
      361 tctgcaatac gcttttggaa gcagaggacg gagtcaggac gcttatgctg gataagaaat
      421 ttggctcggc cggcaatcag atcgttattg aggaatatat gacaggacgt gaggtatccg
      481 tgctttctta tgtggatgga aagacaatca gaacgatgac ttccgcacag gatcataaac
      541 gtgcactgga tggtgatcag ggcttaaata cagggggcat gggaactttt tctccgagtc
      601 ctttctatac ggaacaggta gatgcctttt gcaaagagca tatctatcag aagacggtag
      661 atgcaatggc ggctgaaggc agggctttta agggaattat ttttttcggt ctgatgctga
      721 caaaagatgg accaaaggta cttgaatata atgcgagatt tggcgatccg gaagcgcagg
      781 tcgttttgcc gagaatgcag aatgatatga ttgaggtcat ggaagcatgc atcgacggca
      841 gacttgacga agttgacctg gaatttgcag ataatgcggc agtatgtgtg gtactggcat
      901 cggaaggcta tcccgtacat tatgagaaag gctatccgat tacggggctg gacagattta
      961 aagaagaaaa ggggtatttt gcttttcatg caggaacaaa gtcagagaac ggcaggatcg
     1021 tgacaaatgg aggccgggtg cttgggatca ctgctgtcgg tgaggatctg acaaaagcga
     1081 gaaaaaatgc gtatgaggcg gtacaatggg tctcttttga aaagcagtac tgcagaagtg
     1141 atattggaaa agcgatagaa gaggcataga taacaaagcc gttgcagtct gttgtggggg
     1201 agactgtgaa tacggaatgc agccaagtga gcgtaagggg gtattttatc atgggcctgg
     1261 atctaaatac aatcaaatac ggcagaccta ccgcctgcag taagtgcggc ggagagttat
     1321 tgtatacggg tgtcggtgag tatgag
//
LOCUS       DAOSYE010000742         1345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90684_length_1345_cov_3.536434, whole genome shotgun sequence.
ACCESSION   DAOSYE010000742 DAOSYE010000000
VERSION     DAOSYE010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90684_length_1345_cov_3.536434"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLU43_11120"
     CDS             complement(<1..406)
                     /locus_tag="PLU43_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29004.1"
                     /translation="MRENTKSKVVKILIYLFLILMAVIYLAPLIWMFSVSLKTNAEIF
                     RSPFALPEVIQLGNYIFAWTAGKLGVATLNSTVVCVITLVLTMIIGSMASFAIGRMKW
                     KLSGVVMVYFLTGMMIPIHCILIPLFKTFSSFG"
     gene            complement(420..1307)
                     /locus_tag="PLU43_11125"
     CDS             complement(420..1307)
                     /locus_tag="PLU43_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29005.1"
                     /translation="MDKLLRDKKAICIFVAPGIILFTFVLFIPICQSVYYSLCDYDAL
                     TTPVFVGLKNYIKLFTNDKTMGIALKNSIFFMIFSVVSQLIVGLILAGLLTNINRGRN
                     FFKNVYYLPCVLSSAALGLLWMFIFSTKLGINQILALWGIQGPLWLMDVKDWITLPMR
                     VIAFVALWQYVGQSMMLYMAQIASIDKSLFEAAYIDGASRAKAFRFVTLPLIKPMVAT
                     AMSLNCIGSLKFFDLVFNMTEAGPNHQTEVLATHLYTQGFKYFKYGYASAIGVVLLVL
                     CLIVTFLINKFVKTDSYEM"
BASE COUNT          449 a          308 c          241 g          347 t
ORIGIN      
        1 gaccaaaact ggaaaacgtc ttgaacaggg gaatcagaat acagtggatc ggtatcatca
       61 ttcctgtcag gaagtaaacc atgaccactc cggacagctt ccatttcatg cgtccgatcg
      121 caaaagatgc catggagcca ataatcatcg tcagtaccag cgtgatcaca cagacaaccg
      181 tggaatttaa tgttgccact ccaagttttc ctgcagtcca tgcaaaaata tagttgccca
      241 gctgtattac ttcaggaagc gcaaacgggg acctgaaaat ttcagcattt gtcttaagtg
      301 aaaccgagaa catccagatc agcggtgcca gataaatgac cgccatcaga ataagaaaca
      361 gatagatcaa tattttaacg actttggatt ttgtattttc cctcattttc cgcacctcct
      421 tacatttcat aactgtctgt cttgacaaac ttatttatca gaaaagtcac tatcagacac
      481 aaaacaagaa gtaccactcc gatagcgctt gcatatccat atttaaaata tttaaagccc
      541 tgcgtataaa gatgtgtggc aagaacctct gtctgatgat tgggtcccgc ctccgtcata
      601 ttaaacacaa gatcgaaaaa cttcaaagaa ccgatacaat tcaaagacat tgccgttgcc
      661 accattggct tgatcagcgg cagtgtgaca aacctgaacg cttttgccct tgaagcaccg
      721 tcaatatagg cagcttcaaa cagcgatttg tcaatgctgg cgatctgcgc catataaagc
      781 atcatagact gaccaacata ctgccacagt gcgacaaatg caataactct catcggaagc
      841 gtgatccagt ccttaacatc catcagccac agcggcccct gaattcccca cagggcaaga
      901 atctgattaa taccgagctt tgtgctgaaa ataaacatcc ataaaagacc aagtgctgcg
      961 gaagaaagta cacacggcag ataatagacg tttttgaaga aattccttcc tctgttaata
     1021 ttggtaagca gtccagcaag gatcaaacct acgatcagct gtgaaacaac cgaaaaaatc
     1081 ataaaaaaga tggaattttt caatgcaatt cccatcgttt tatcatttgt aaaaagcttg
     1141 atataatttt ttaatccaac aaacaccggg gtcgttaatg catcataatc acatagggaa
     1201 taatagaccg actgacagat cggaataaac agaacaaatg taaataaaat aattcccggt
     1261 gctacgaaaa tacatatggc cttcttgtct cgcaatagtt tatccatatt attcctccaa
     1321 gcagataatt ttttatttct gtttt
//
LOCUS       DAOSYE010000743         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_90982_length_1343_cov_2.434006, whole genome shotgun sequence.
ACCESSION   DAOSYE010000743 DAOSYE010000000
VERSION     DAOSYE010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90982_length_1343_cov_2.434006"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLU43_11130"
     CDS             complement(<1..609)
                     /locus_tag="PLU43_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO29006.1"
                     /translation="MIVEKKCKKQLFLFMLSVMTFLLPVMLFTGCKKEKKTEIVLTTD
                     FEDGEVFRIKSSSCMLPEIMVYLVNSENQYDDIFGSQIWQVPVGSSTMEKEYKETIIA
                     RIAQIKVMNLLAPQYNIVLDEDEEAKVKAAARDYYSSLNQSEIDLLGADEETIYGMYH
                     EFAVADKLYHAITDEINPEISDDEARIITVKSILIKTYSTDSS"
     gene            complement(614..>1343)
                     /locus_tag="PLU43_11135"
     CDS             complement(614..>1343)
                     /locus_tag="PLU43_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO29007.1"
                     /translation="VDVGVRVEVRDEVMQFLNENLYEAKLIYHTPTFDDKVRTFCTNP
                     SGEVATEYYENGLAVVNGHAYKSQEYKTNNTNFAILVSKNFTKPFKTPIEYGKQIAQL
                     SNMLCGGRTLVQTFGDFKRGRRTTEERLCRNNLMPTLKDAVPGDLSLVFPHRIMVDIE
                     EMIFALDKITPGLASDETLLYGVEVKFYSNKVVVSTDFETSVRGLYAIGDGAGVTRGL
                     QQASANGISAARSILAKYVQESKV"
BASE COUNT          332 a          370 c          257 g          384 t
ORIGIN      
        1 ggacgaatct gtcgaatagg tcttgatcag aatactcttc accgtaatga tgcgtgcttc
       61 atcatcgctg atctccggat ttatttcatc ggtgatcgca tgatacagtt tgtccgcgac
      121 tgcaaattca tgatacatcc cataaatcgt ttcctcatcc gcgccaagca gatcgatctc
      181 gctttggttc agactggagt aataatctct ggccgctgcc ttcacttttg cttcttcatc
      241 ctcgtcaagc acaatattgt actgcggtgc aagcagattc atcactttga tctgcgcgat
      301 ccgcgcgatg atcgtttctt tatattcctt ctccattgtg ctgctgccta ccggcacctg
      361 ccagatctgc gaaccgaata tatcgtcata ctgattttcc gaattgacaa ggtataccat
      421 gatttcaggc agcatacacg aactggactt aatacggaac acttcgccat cctcaaaatc
      481 cgtcgtcaga acgatctccg ttttcttctc ctttttacag ccggtaaaca gcataacagg
      541 aagcagaaac gtcataaccg acagcataaa cagaaacagc tgttttttac attttttttc
      601 aactatcatt taatcaaacc tttgattctt gcacatattt tgccagaata ctgcgcgctg
      661 cgctgatccc gtttgcagaa gcctgctgca ggcctcttgt tacacccgcc ccgtctccga
      721 tcgcatacag acccctgaca cttgtctcaa aatccgtgct gactacgacc ttattggaat
      781 aaaacttaac ttccacgcca tagagcagcg tttcatcgct tgcaagtccc ggcgtgatct
      841 tgtcgagtgc aaagatcatc tcttcaatat cgaccatgat acgatgcgga aagacaagcg
      901 acaaatctcc agggacagcg tccttcagtg tcggcatcag attgttcctg caaagacgct
      961 cttccgttgt ccgtctgccc cgtttgaaat ctccaaacgt ctgcacaagg gtccgccctc
     1021 cgcagagcat attggaaagc tgcgcgatct gtttgccgta ttcgatcggc gttttgaacg
     1081 gctttgtaaa attcttggac actaaaatcg caaaattcgt attattggtc ttatactcct
     1141 gagatttata cgcatgcccg ttaacgaccg caagtccgtt ctcataatat tccgtagcga
     1201 cctctcctga cggatttgta cagaacgtac gcaccttatc gtcaaatgtc ggcgtatggt
     1261 agatcagctt tgcctcatac agattctcgt tcaaaaactg catgacttca tccctcactt
     1321 ctacgcggac accaacatcc acc
//
LOCUS       DAOSYE010000744         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91279_length_1340_cov_3.184436, whole genome shotgun sequence.
ACCESSION   DAOSYE010000744 DAOSYE010000000
VERSION     DAOSYE010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91279_length_1340_cov_3.184436"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLU43_11140"
     CDS             complement(<1..395)
                     /locus_tag="PLU43_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29008.1"
                     /translation="MKEKAMDTKLVLRPIVGYLTHSHFWEGPCRAGHKEDMTQEAESR
                     AADTAFYHAKEEMKNVSDKAQLLDAIDCRYDEKFVVSKEMFDKIEEDIDKVDFFLCMG
                     WRIPKLERYRKPIIIMQNGNEGIDFSAYCR"
     gene            complement(509..>1340)
                     /locus_tag="PLU43_11145"
     CDS             complement(509..>1340)
                     /locus_tag="PLU43_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOO29009.1"
                     /translation="GWLRFIKSRHPHLVPRLSTAKSPQQMFGAVMKSYFAEMIGVKPE
                     NLVTISVMPCVAKKEESKMELFYEEYAGNDVDVVLTTRELNRMIRTAHIDTTVLKERA
                     CDLPMQEGTGAGVIFGTTGGVMEAALRSAYYIITGSNPEPDAFSEVRSTREREGYKEA
                     VFKIGDAVIKTAVVSGLQNIEYLLELLESGKVQYDFVEVMACPGGCSGGGGQPIHDGM
                     ELATPRGKYLYELDEKASIRYSHENVCVNRIYREFFEKPLSHKAHMLLHTDHNSWEMP
                     "
BASE COUNT          322 a          372 c          243 g          403 t
ORIGIN      
        1 cggcaatagg cggaaaaatc aattccttca tttccattct gcataatgat gatcggtttt
       61 ctgtaacgct ccagctttgg gatcctccag cccatgcaga ggaaaaaatc gactttgtcg
      121 atgtcctctt ctattttgtc gaacatttct tttgatacaa caaatttctc gtcataccgg
      181 cagtcgatcg catccagaag ctgcgcctta tccgatacgt tcttcatttc ttcttttgcg
      241 tgataaaatg cggtgtccgc agccctgctc tcagcttcct gcgtcatatc ctctttatgt
      301 cccgcacggc acggaccttc ccaaaaatga gaatgtgtca ggtatcctac gatcggcctt
      361 aacaccagtt ttgtgtccat tgctttttct ttcataaaaa atccctccag attgtctgat
      421 agttttaacc aattccatcc tatcaaacaa tccaaagggt ttccatgcac tttcttatca
      481 taaaaaatgt acttttttca ccgcaggatc agggcatttc ccatgagtta tgatccgtat
      541 gcagcagcat atgcgcctta tgagacagcg gcttttcaaa gaattccctg tagatccggt
      601 tcacacatac attttcatgc gaatatctga tggacgcttt ctcatccagt tcatacagat
      661 attttccacg aggcgttgcc agctccattc catcgtggat cggctgtccg ccgcctccgg
      721 agcatccgcc cggacaggcc atgacttcta caaaatcata ctgtacctta ccgctctcaa
      781 gcagctccag taaatactcg atattctgta atccgctcac aactgctgtt ttgatcacag
      841 catccccgat cttgaaaaca gcctctttat atccctcgcg ttctcttgtg cttcttactt
      901 cactaaaagc atccggttcc ggattgcttc ctgtaatgat ataatatgcg gaacgcagtg
      961 ccgcctccat aacgccgccg gtcgttccaa agatcacacc ggctcctgtt ccttcctgca
     1021 tcggaagatc acaggcacgt tccttcaata cggtcgtatc aatatgcgcg gttctgatca
     1081 ttctgttcaa ttccctcgta gtcagtacga catccacatc atttcctgca tattcctcat
     1141 agaacagctc cattttggac tcttcttttt tagctacaca cggcataacc gatatcgtta
     1201 cgagattttc cggttttaca ccgatcattt ccgcaaaata ggacttcatt accgcgccaa
     1261 acatctgctg cggagatttt gccgttgaaa gccttggtac aagatgcgga tgacgggatt
     1321 tgataaaccg aagccatccc
//
LOCUS       DAOSYE010000745         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91288_length_1340_cov_2.962646, whole genome shotgun sequence.
ACCESSION   DAOSYE010000745 DAOSYE010000000
VERSION     DAOSYE010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91288_length_1340_cov_2.962646"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLU43_11150"
     CDS             complement(<1..199)
                     /locus_tag="PLU43_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29010.1"
                     /translation="MKNRMRNGCMVVLFGLLFVLIGCSMGAQDAQLDFGKNQVSEKTV
                     AVSAVNASEEEIPDGLTSELYD"
     gene            complement(448..>1340)
                     /locus_tag="PLU43_11155"
     CDS             complement(448..>1340)
                     /locus_tag="PLU43_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOO29011.1"
                     /translation="KKEYYIMKINVKTISRWKLLLDVAMITVLSLMYNHHALGMTFHE
                     VGGLILIGVFVIHILLNRKWVAGITKKLFQSGPAVRTKISYVINLLLVISFLIVGISG
                     ILISKVVFHLNISTGGINWKGLHIGAAALAMVLVGIHAGMHNSYITGILKKMIPLPKA
                     VAKGIGICLFLLFVGYGAYSIQENNFIGMLERPFISQSEGGAANRGQESEMEEDLDQA
                     AVSEAGFSDDMESAGEIQNHEGSGSGTGGGRQSGLTAAIQTFTGYGSIAMFFACATIL
                     IEWLIGIFGNKRRKQKIKEK"
BASE COUNT          392 a          333 c          192 g          423 t
ORIGIN      
        1 catcatacaa ttctgatgtc agcccatccg gaatttcttc ttctgatgca tttaccgccg
       61 atacggctac agttttttca gagacctgat ttttaccaaa atcaagctgc gcatcctgtg
      121 cgcccataga acatccgatc aatacaaaaa gcagaccgaa tagtactacc atacatccat
      181 ttctcattct gtttttcata tgttcccttt ccctcataaa tatattttaa taacagttcc
      241 cacgcttaca gcagcctgct ctgctgtttg aagattactc ctttcctttg acagtatttt
      301 gcatctatga taattttatt ataatggcta tgttgatttt atgtcaagaa aatgataact
      361 tttcattgag atcatacaaa taaaaaaaca acctgaatgg ctgttacatt caggctgttc
      421 ctctcttctg ctccaaaatc ggttctttta tttctctttt attttctgtt tccttctttt
      481 atttccaaaa attccgatta accattcaat aaggatcgtt gcgcatgcaa aaaacatggc
      541 aatagaacca taacctgtaa aagtctgaat agctgcggtc aaaccgcttt gacgtccgcc
      601 gccggttccg gatcccgacc cttcgtggtt ctgtatctca ccggcacttt ccatatcatc
      661 cgaaaatccg gcttcactta cagcagcctg gtcaagatct tcttccattt cactttcctg
      721 tccgcggttt gcagctccgc cttcactctg actaataaac ggcctctcca gcatgccgat
      781 aaaattattt tcctgaatac tgtaggcacc ataacccaca aataatagga aaagacaaat
      841 accgattccc tttgccacag cttttggcag cggaatcatt tttttcagaa tacctgttat
      901 ataggaatta tgcattcctg catgaatacc aaccaatacc atcgccaaag ctgccgcacc
      961 aatatgaagt cctttccagt ttattccgcc tgtcgaaata ttcagatgaa atacgacttt
     1021 ggaaatcaat atcccgctga ttccaacgat aagaaaagat atgactaaca gcagattgat
     1081 aacgtaactg atttttgtcc gaaccgcagg accactctga aacagttttt ttgttattcc
     1141 tgccacccat ttgcggttca gtaaaatatg aataacaaaa acacctatta aaatcaaccc
     1201 tccgacctca tgaaatgtca tccccaatgc atgatggtta tacattaaag acaaaaccgt
     1261 aatcatcgca acatcaagca ataacttcca tctgctgatt gttttcacat ttatcttcat
     1321 aatataatac tcctttttat
//
LOCUS       DAOSYE010000746         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91311_length_1340_cov_2.456809, whole genome shotgun sequence.
ACCESSION   DAOSYE010000746 DAOSYE010000000
VERSION     DAOSYE010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91311_length_1340_cov_2.456809"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLU43_11160"
     CDS             <1..484
                     /locus_tag="PLU43_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO29012.1"
                     /translation="EESEMDLCGDFEEESAFINHLSEYDHLKNIKEKFLNYCLALSGG
                     IAGKRESVNQKQAVMALDYIEKNYMNACMSLNTVCEYLSVSTSYFSTIFKAYSGETFI
                     ETLTRVRMEKAKKLLETTSLKNYEVALEVGYSDPHYFSSIFKKQVGKTPGEYAKGVRN
                     "
     gene            486..>1340
                     /locus_tag="PLU43_11165"
     CDS             486..>1340
                     /locus_tag="PLU43_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO29013.1"
                     /translation="MHEDRKQFQFGFKSIRMAMLVPFAVLIAAAVLILAFISVQQIEN
                     ALFENSTDYTMQLVTMVNSDIDSYISNMENLAELVLSNASVGSYLYKDEDEFDENTNE
                     QAIEEQFELLRDTRDDIYNIGIVGDNGRYFINNKKVSINPYAELAGMDWYTKAISGEG
                     EITSSHVQNIVLNEYKWVVTLSRGIVNPETGKTEGVLFIDLNYSSISSLCEKISLGAK
                     GYVFILDEDGSVIYHPKQQLLYNGLLDERIDKILSTPKGGNGTFLSDDGKRLYTITKS
                     EKTGLSVVG"
BASE COUNT          423 a          228 c          339 g          350 t
ORIGIN      
        1 tgaagaaagc gaaatggatc tttgcggtga ttttgaagag gaaagtgcgt ttattaatca
       61 tctgagcgaa tatgatcatc tgaaaaatat caaagaaaaa tttctgaatt actgtcttgc
      121 gctttcggga ggcattgccg gcaagcggga aagcgtcaat cagaagcagg ctgtcatggc
      181 acttgactat attgagaaga attatatgaa tgcatgtatg tcgttgaata cggtatgtga
      241 gtatctgagt gtgagtacaa gttatttcag tacgatcttt aaagcatatt caggagaaac
      301 ctttattgag acactgaccc gtgtcagaat ggaaaaggca aagaagcttc ttgaaacgac
      361 aagtctgaaa aactatgagg tggcattgga agtcggttac agtgatccgc attatttcag
      421 cagcatcttt aaaaagcagg taggaaaaac accgggggaa tacgcgaagg gcgtaagaaa
      481 ttaatatgca tgaagacaga aagcagtttc aatttgggtt taaaagtatc agaatggcca
      541 tgcttgtacc gtttgcggta ctgattgcgg cagccgttct gattttggcg tttatttccg
      601 ttcagcagat agaaaacgca ttatttgaaa attccacgga ctatacgatg cagcttgtga
      661 cgatggtcaa ttccgatatt gattcgtata tatccaatat ggagaacctg gctgagcttg
      721 tgctgagcaa tgcaagcgtc ggcagttatc tttataagga tgaagatgaa ttcgacgaga
      781 atacgaacga gcaggcgatc gaagagcagt tcgagctttt gcgggatacg cgggatgata
      841 tttataacat aggaatcgtc ggagataatg gccgctattt cataaacaat aaaaaggttt
      901 cgatcaatcc atatgcggag cttgcaggaa tggactggta tacgaaggca atatcaggag
      961 aaggggagat cacgtcttct catgtacaga atattgtatt aaatgaatac aaatgggtag
     1021 taacgctgag ccgcggaatc gtaaatccgg aaaccggaaa aacagaaggc gtgcttttta
     1081 ttgacctgaa ttattcttcg atcagcagtc tgtgtgagaa gatcagcctc ggggcaaaag
     1141 gctacgtttt tattctggat gaagacggca gtgtcatcta tcatccaaag cagcagcttt
     1201 tatacaacgg actgcttgac gagcggatcg acaaaatatt aagtacacct aaaggaggca
     1261 atgggacttt tttgtcggac gacggcaaac gtctttatac gatcacaaaa tccgaaaaaa
     1321 ccggcttgtc ggtagtcggc
//
LOCUS       DAOSYE010000747         1339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91383_length_1339_cov_3.708723, whole genome shotgun sequence.
ACCESSION   DAOSYE010000747 DAOSYE010000000
VERSION     DAOSYE010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91383_length_1339_cov_3.708723"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PLU43_11170"
     CDS             <1..100
                     /locus_tag="PLU43_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HOO29014.1"
                     /translation="KAPRGTRIFGPVARELREKQFMKIVSLAPEVL"
     gene            113..424
                     /gene="rplX"
                     /locus_tag="PLU43_11175"
     CDS             113..424
                     /gene="rplX"
                     /locus_tag="PLU43_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO29015.1"
                     /translation="MATMKIKKGDTVKVIAGKDIGAEGKVLLINKEKGKVLVEGINMV
                     KKHTKPSVQNQNGGIVEKEAYIDVSNVMYLHKGKPTRIGFKVENDKKVRFAKSTGEVI
                     D"
     gene            448..987
                     /gene="rplE"
                     /locus_tag="PLU43_11180"
     CDS             448..987
                     /gene="rplE"
                     /locus_tag="PLU43_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO29016.1"
                     /translation="MSRFRDMYKDQIVDAMIKKFEYKNVMEVPKLDKIVVNMGVGEAK
                     ENAKLLEAAVKDLETITGQKAVTTKAKHAIANFKIREGMAIGCKVTLRGEKMYEFLDR
                     LVNLALPRVRDFRGVNPNAFDGRGNFALGIKEQLIFPEIEYDKVDKVRGMDIIFVTTA
                     KTDEEARELLAQFNMPFAK"
     gene            1003..1188
                     /locus_tag="PLU43_11185"
     CDS             1003..1188
                     /locus_tag="PLU43_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOO29017.1"
                     /translation="MAKTAMKVKQQRKQKFSTREYSRCKICGRPHAYLRKYGICRICF
                     RELAYKGQIPGVKKASW"
     gene            1207..>1339
                     /gene="rpsH"
                     /locus_tag="PLU43_11190"
     CDS             1207..>1339
                     /gene="rpsH"
                     /locus_tag="PLU43_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO29018.1"
                     /translation="MTMSDPIADMLTRIRNANVAKHDTVDIPSSKMKISIADILYNEG
                     "
BASE COUNT          466 a          212 c          339 g          322 t
ORIGIN      
        1 caaggctccg agaggaactc gtatttttgg acctgtagcc agagagcttc gtgaaaaaca
       61 gtttatgaag attgtttccc tggctcctga agtattatag gaggtgcgaa ggatggcaac
      121 tatgaagatt aaaaaaggcg atacggtcaa ggttatcgca ggcaaggata tcggagcaga
      181 aggcaaggtt cttctcatta ataaggaaaa aggcaaggtt cttgtagaag gcatcaatat
      241 ggtgaagaag catacgaagc cttccgtgca gaatcaaaac ggcgggatcg ttgagaagga
      301 agcgtatatc gatgtatcca atgtgatgta tctgcacaag ggaaaaccga ccaggatcgg
      361 atttaaggtt gaaaatgaca aaaaagtacg ttttgcaaaa tcaaccggcg aagtaattga
      421 ttaattcaag agaggaggtc taagacgttg agtagattca gagacatgta taaagaccag
      481 atcgtagatg ctatgatcaa aaagtttgaa tataaaaatg ttatggaagt accaaagctt
      541 gacaagatcg ttgtcaatat gggcgtcggt gaagcgaaag aaaatgcaaa gcttttggaa
      601 gcggccgtaa aagatcttga aacaataaca ggacaaaagg cagttacaac aaaagcaaag
      661 catgccattg ccaattttaa gatcagagag ggtatggcga tcggctgtaa agtaacgctg
      721 cgcggtgaga aaatgtatga atttcttgat cgcttggtaa atcttgcgct gccgcgtgta
      781 cgtgatttca ggggagtgaa tccgaatgcg tttgacggca gaggcaattt tgcactcggc
      841 attaaagagc agctgatttt tccggagatc gagtacgata aggttgataa agtaagagga
      901 atggacatta tttttgttac aaccgcaaag accgatgaag aagcacgtga attattggca
      961 cagtttaata tgccgtttgc aaagtaaaag gaggaatcgt tcatggctaa aacagcaatg
     1021 aaggtaaaac agcagcgtaa acagaagttt tcgacgcgcg aatacagccg ttgtaagatt
     1081 tgcggtcgtc cgcatgcata tttaagaaaa tacggaattt gcagaatatg cttccgtgaa
     1141 ttagcttata aaggacagat accgggcgtt aaaaaagcaa gctggtaaga aaaggaggca
     1201 atctaaatga caatgagtga tccaattgca gatatgctta caagaatccg taatgcaaat
     1261 gttgcaaaac acgatacagt agatattcct tcatcaaaaa tgaagatttc catcgcggat
     1321 attctttata atgaaggtt
//
LOCUS       DAOSYE010000748         1339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91427_length_1339_cov_2.319315, whole genome shotgun sequence.
ACCESSION   DAOSYE010000748 DAOSYE010000000
VERSION     DAOSYE010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91427_length_1339_cov_2.319315"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLU43_11195"
     CDS             <1..849
                     /locus_tag="PLU43_11195"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOO29019.1"
                     /translation="YTIAVGSTGNLGLSIGIAAAAFGFHAVVHLSQDAKEWKKQELKK
                     RGVTVVEYAGDYEKAVEEGRKAAEAEEYTYFIDDENSKILFSGYTTAAWELAEQLAQQ
                     QIITDRQHPLFVYLPCGVGGGPGGITFGLKQIFGSSVHCFFAEPTHAPCMMLSLATGR
                     GRELGIKDFGLDNKTEADGLAVGRASGFAAAVMGPMLDGCYTVGDEKLFAYLKLLAKS
                     ENKKLEPSALAGMGGPYQLFGTKRGKEYLEENGIEDGEEITHVVWATGGGLVPEDVMR
                     TYLSTG"
BASE COUNT          420 a          229 c          369 g          321 t
ORIGIN      
        1 tatacgattg cagtcggttc taccggaaat ttgggacttt ctatcggtat tgcggctgca
       61 gcgttcggtt ttcatgcggt cgtgcatttg tcacaggatg caaaggaatg gaaaaagcag
      121 gagcttaaga aaagaggcgt tacggtcgtc gaatatgccg gcgactatga aaaagcagtt
      181 gaagaaggca gaaaggcagc ggaagcggaa gaatacacct attttatcga cgatgaaaat
      241 tctaaaattt tattttccgg ctatacaact gctgcgtggg agcttgcaga gcagctggcg
      301 cagcagcaga tcataacgga caggcagcat ccgctgtttg tctatctgcc atgcggagtc
      361 ggcggcggtc cgggagggat aacgtttggt ttaaaacaga tattcggttc gagtgtgcat
      421 tgtttttttg cggaaccaac gcatgcgccc tgtatgatgc tctcgcttgc aacaggccgc
      481 ggccgcgaac tgggtatcaa ggattttgga ctggataata aaacggaggc agatgggctt
      541 gcggtcggaa gagcttccgg atttgcggct gccgtaatgg gaccaatgct tgacggctgc
      601 tatacggtcg gggatgaaaa gctgttcgcc tatttaaagc ttttggctaa gagcgagaat
      661 aaaaaactgg agccaagcgc tctcgcagga atgggaggac cttaccagct ctttggaaca
      721 aagagaggaa aggaatatct tgaagaaaat ggaatagagg acggcgagga gatcacacat
      781 gttgtatggg cgaccggcgg cgggcttgtg ccggaagatg tcatgcggac atatcttagc
      841 acaggatgaa aggaacaggg atataaaagg tatagaggta taaacaaaat aaaaagaagg
      901 cggtgattat ttcgtgaacc gccttctttt tatttgatca acagtaaaaa ggatgaaatt
      961 ttcgataatc gtaaattata ggaaaaagta ataataaccg attaacatat ttgtaaaggc
     1021 tttaataata gtatattatg cacaattaaa gtataaaata actaggtatt attcacaaat
     1081 atatatcaac cggttgacac ataaattaaa tcgtagtatc gtatagttac aaccaagcgc
     1141 aggttgtgga ttgtaacgta tggatctagt acggaatcag gggacggcat gcagaatagt
     1201 ctgctgccgg tagcaatgga aacatagcgg aggaaaaaaa tgaaagtaaa aaaatggatg
     1261 gtattattat catcagtaat ggtaacagcg cttttggcag gatgcggaag cagcgcagca
     1321 cagagtgcac agacagagg
//
LOCUS       DAOSYE010000749         1337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91669_length_1337_cov_2.186427, whole genome shotgun sequence.
ACCESSION   DAOSYE010000749 DAOSYE010000000
VERSION     DAOSYE010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91669_length_1337_cov_2.186427"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLU43_11200"
     CDS             complement(<1..320)
                     /locus_tag="PLU43_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOO29020.1"
                     /translation="MTVTKVEDYINGRYKIDIDGAFAFVLYKGELRRYQIKEGELLSG
                     EAYREIMESVLPKRAKLRAMHLLKVSDRTEADVRRRLKDGCYPEQAIDEAIIYLKSYH
                     YIDDR"
     gene            complement(383..>1337)
                     /gene="recA"
                     /locus_tag="PLU43_11205"
     CDS             complement(383..>1337)
                     /gene="recA"
                     /locus_tag="PLU43_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOO29021.1"
                     /translation="TVPTGSLSLDIALGLGGIPKGRIVEIYGPESSGKTTVTLHMIAE
                     VQKRGGIAGFIDAEHALDPVYAKNIGVDIDNLYISQPDNGEQALEITETMVRSGAVDI
                     VVVDSVAAIVPKAEIDGEMGDSHVGLQARLMSQALRKLTAVISKSNCTVIFINQLREK
                     IGVMFGNPETTTGGRALKFYSSIRLDVRRIETLKQGGEVVGNRTRVKVVKNKIAPPFK
                     EAEFDIMFGKGISAEGNILDLATEVDIVNKSGAWYAYDSQKIGQGRENAKQYLMENPT
                     VCAEIEEKVRKHFGLIQKGSAAAETAAEKSAADSKKAKTSK"
BASE COUNT          326 a          375 c          247 g          389 t
ORIGIN      
        1 cggtcgtcga tataatgata actttttaaa tatatgatcg cctcatcgat tgcctgttca
       61 ggataacagc catccttaag cctgcgccgc acatccgcct ccgtcctgtc cgacaccttt
      121 aacagatgca ttgcacggag ctttgcacgt ttcggcaaaa cactttccat gatttcacga
      181 taagcttccc ctgaaagcaa ttcaccttct tttatctgat accgacgcag ttccccctta
      241 tataatacaa aggcaaacgc accgtctata tcgatcttat atctgccgtt aatatagtcc
      301 tcaacttttg taactgtcaa acactctctg tcctttcctt gtcgtcatcc aaatggaaca
      361 gccggttctg ttaaaaaagc tgttattttg atgtttttgc ttttttactg tccgcagcgg
      421 atttttccgc agccgtttcc gcagcagccg atcctttctg aataagtccg aaatgcttac
      481 gtaccttttc ctcgatttca gcgcatacgg tcggattttc catcagatac tgctttgcat
      541 tttcacggcc ctgaccgatc ttctgactgt cataggcgta ccatgccccg ctcttattta
      601 caatatcgac ctccgtagcc agatcaagaa tgtttccttc ggcagaaata cctttcccaa
      661 acataatatc aaattccgct tccttaaaag gcggtgcgat cttgttttta acgaccttga
      721 cccgtgtacg gttgccgaca acttctccgc cctgcttcaa agtctcgata cgtctgacat
      781 ccagccggat cgaggagtaa aacttcagcg cccgtccgcc tgttgtcgtc tccggattgc
      841 cgaacataac accgatcttt tcgcgtaact gatttataaa aataaccgta cagtttgatt
      901 tactgatcac tgccgttaat ttacgcaatg cctgagacat cagcctggct tgcaggccca
      961 catgggagtc acccatttct ccgtctattt cagcctttgg aacaattgcc gccaccgaat
     1021 cgacaactac aatatcaacg gcaccggaac gtaccatcgt ttccgtaatc tcaagagcct
     1081 gttcaccgtt atccggctgt gagatataaa ggttatcaat atccaccccg atatttttgg
     1141 catatacagg atcaagtgca tgctccgcat caataaaacc ggcaattccg cccctttttt
     1201 gtacttcagc aatcatatgc agggtaacgg ttgtctttcc gctggattcc ggaccataaa
     1261 tttcaacgat cctgcccttg gggataccgc cgagtccaag cgcaatatca agacttaggg
     1321 aaccggtcgg aaccgtc
//
LOCUS       DAOSYE010000750         1336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91728_length_1336_cov_3.634660, whole genome shotgun sequence.
ACCESSION   DAOSYE010000750 DAOSYE010000000
VERSION     DAOSYE010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91728_length_1336_cov_3.634660"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="pheS"
                     /locus_tag="PLU43_11210"
     CDS             <1..704
                     /gene="pheS"
                     /locus_tag="PLU43_11210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO29022.1"
                     /translation="RHPNFIVMEEVERIFVGMGYEVVEGPEIETDYYNFEALNIPADH
                     PAKDEQDTFYINDSFLLRTQTSGTQVHEMEKGKLPIRMIAPGRVFRSDEVDATHSPSF
                     HQIEGLVIDKKITFSDLKGTLAEFAREMFGEDTKVRFRPHHFNFTEPSAEADVSCFKC
                     KGKGCRFCKGTGWIEILGCGMVHPNVLRMSGIDPEEYSGFAFGLGLERIALLKYEIDD
                     MRLLYENDIRFLKQF"
     gene            729..>1336
                     /locus_tag="PLU43_11215"
     CDS             729..>1336
                     /locus_tag="PLU43_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO29023.1"
                     /translation="MNTALSWIKAYVPGLGCTDQEYTDKMTLSGTKVETFERLDKNLK
                     DIYIGQIQSIEKHPDADKLIVCQVDMGDRTLQIVTGAPNVQTGDKVPVVIAGGKVAGG
                     HDGGPLPENGIAINAGKLRGVESFGMMCSIEELGASREMYPEAPEDGIYIFDKDAEVG
                     ADAIEELGLRDSVFEYEITSNRVDCYSVLGIAREAAATFHEP"
BASE COUNT          385 a          247 c          376 g          328 t
ORIGIN      
        1 atcgccatcc taattttatt gttatggagg aagttgagcg tatttttgtc ggcatgggat
       61 atgaggtcgt agaaggtcct gagatcgaga ccgattatta taactttgag gctttgaata
      121 ttccggcgga tcatccggca aaggatgaac aggatacttt ttacataaac gacagttttc
      181 tgcttcgtac gcagacttca ggcacacagg tgcatgagat ggaaaaaggg aaattaccga
      241 tcagaatgat cgcacccggc agggtgttcc gttccgacga agtggatgca acgcattctc
      301 cctcttttca ccagatagaa ggccttgtta ttgataaaaa gattactttt tccgacttaa
      361 aggggactct tgccgaattt gcaagagaaa tgtttggaga agacacaaag gttcgcttca
      421 gaccgcatca ttttaatttt acagaaccga gtgcggaagc agatgtctcc tgttttaagt
      481 gcaagggaaa gggatgcagg ttctgtaagg gcaccggctg gatagaaata ctgggatgcg
      541 gcatggtgca tccgaatgtt ctgcgtatga gcggcattga tccggaggag tattccggtt
      601 tcgcttttgg tctggggctg gaaaggatcg cattgctgaa atacgagatt gatgacatgc
      661 ggctgcttta tgaaaacgat atcaggttct tgaaacagtt ttaataaaaa agaaagggat
      721 aaatcattat gaatacggca ttatcatgga tcaaagcata tgtacccggg cttggctgca
      781 cggatcagga atatacggat aagatgacgt tatcaggaac aaaggtagaa acctttgaac
      841 gtctggataa aaatttaaaa gatatctata tcggacagat tcagtcgatc gaaaagcatc
      901 cggatgcgga taagctgatc gtctgtcagg tggatatggg agacaggacg cttcagatcg
      961 taacaggggc accaaatgta cagacaggag ataaagtacc ggtagtgatc gcaggcggca
     1021 aggtcgcagg cggacacgac ggcggtccgc ttccggaaaa cgggattgct attaacgccg
     1081 gcaagttaag aggcgtggag tcgttcggca tgatgtgttc gatcgaggaa ctgggagctt
     1141 ccagggaaat gtatccggaa gcgccggaag acggcattta tattttcgat aaagatgcgg
     1201 aagtaggagc ggatgcgatc gaggaacttg gactgcgcga cagtgtattt gaatatgaaa
     1261 tcacgtccaa cagagtcgac tgctacagtg tgcttggaat agcaagagag gcggcagcca
     1321 cgtttcatga gccctt
//
LOCUS       DAOSYE010000751         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91831_length_1335_cov_3.495312, whole genome shotgun sequence.
ACCESSION   DAOSYE010000751 DAOSYE010000000
VERSION     DAOSYE010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91831_length_1335_cov_3.495312"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLU43_11220"
     CDS             complement(<1..265)
                     /locus_tag="PLU43_11220"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287436.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO29024.1"
                     /translation="MEQLTMSKYHGVGNDYLVVDPNVCEIELTAEKIKKICDRNFGAG
                     SDGILYGPVFKNDRIYVKIYNPDGSEAEKSGNGVRIFGKYLLDK"
     gene            409..1230
                     /locus_tag="PLU43_11225"
     CDS             409..1230
                     /locus_tag="PLU43_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29025.1"
                     /translation="MQKSGYLYQNFRLFHLRDKRDRKFDYHYHDFNKIIIFLSGNVTY
                     YIEGKAYVLKPWDILLVNNHAIHKPVISPDVYYERIVIWIDEDFMKNTTTDITACFQK
                     ASERSFNLIRLGSGLIDRLKNTVRDLELSLSENENEFGHTLLTDSLFMELMVYINRIY
                     LGKKYESEPDALKYDKRIEEILKYIHLHLSEEISIDLLADSFYMNKYHLMHKFKEETG
                     YTVHSYILQKRLLYVSRLHSTGTPITKAALAGGFKDYSTYYRAVKRENCSRHADS"
     gene            complement(1258..1329)
                     /locus_tag="PLU43_11230"
     CDS             complement(1258..1329)
                     /locus_tag="PLU43_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOO29026.1"
                     /translation="MSKKYGLYRQNYCGCVYSRKKEE"
BASE COUNT          398 a          303 c          245 g          389 t
ORIGIN      
        1 ccttatccaa caggtatttc ccgaagatcc tgacaccatt ccctgatttt tcggcttccg
       61 aaccatccgg attataaatt ttcacataga tccgatcgtt tttaaaaaca ggaccgtata
      121 aaataccatc cgaacccgcg ccaaaatttc ggtcacatat ttttttgatt ttttccgctg
      181 tcagttctat ctcacagaca tttggatcaa ctacaagata atcatttccc actccgtgat
      241 atttactcat tgtcaactgt tccataccag gcatcctctc ctttatctga tctgcaatta
      301 ttataatata ttgaatctgc aatttacatt gtttgaaatg ttaaaaagtt tgcaaaaaat
      361 gctgcctcat gatatgctaa gtaaaagcca tcggaaagga ggggcagcat gcaaaaatcc
      421 ggttatcttt atcaaaattt tcgtcttttt catcttaggg ataagagaga ccgcaaattc
      481 gactatcatt atcatgactt taacaagatc atcatatttc tttcaggtaa tgttacatac
      541 tacatcgaag gcaaggccta tgtcctgaaa ccatgggata ttctccttgt gaataatcac
      601 gcaatacaca agccggttat ctcccccgac gtttactatg aacggatcgt gatctggatc
      661 gatgaagatt ttatgaaaaa cacaactacc gatattacgg catgttttca gaaagcttcc
      721 gaacgcagct ttaatttgat ccgccttgga agcggtctga tcgaccgctt aaaaaatacg
      781 gtgcgtgatc tggaactgag tctttcggaa aatgaaaatg aattcggtca cacattgctg
      841 acagattctc tttttatgga actgatggtc tatatcaatc gcatttacct cggcaaaaaa
      901 tatgagagtg aaccggatgc ccttaaatat gacaaacgga tcgaagaaat actgaagtac
      961 attcatcttc acctttccga agagatttcc attgacctgc ttgccgattc cttttatatg
     1021 aataagtacc acctgatgca caaattcaag gaggagaccg gatataccgt acacagctat
     1081 attttacaaa agcgcctttt gtacgtaagc aggctgcact ctacgggaac gcccattaca
     1141 aaagccgccc tggcaggcgg cttcaaagac tattcgacct attatcgtgc ggtaaaacgg
     1201 gaaaactgca gcaggcatgc ggattcctga acaggcatgc agtacagcag aatggagtta
     1261 ctcctctttt tttctggagt atacgcagcc gcaatagttc tgccgataca gcccgtactt
     1321 ctttgacagc tcgat
//
LOCUS       DAOSYE010000752         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_91870_length_1335_cov_2.548437, whole genome shotgun sequence.
ACCESSION   DAOSYE010000752 DAOSYE010000000
VERSION     DAOSYE010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91870_length_1335_cov_2.548437"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..>1335)
                     /locus_tag="PLU43_11235"
     CDS             complement(116..>1335)
                     /locus_tag="PLU43_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29027.1"
                     /translation="ESRTIKILSMWPEDDSSTTANGSIITAMCEQYKADVDPNFTWEF
                     EYVDSDNLKTKVQTLAASDDLPDIFAYDAGTPLVDLIDADLVLDVTQALTDAGVYDKL
                     DNGATTYLAALTGTDDIYDLPLGLNIEGFWYNKALFEQAGITEAPTTWDEMLADADLL
                     LAAGIQPFATGAGDSWPATRLIHAYAIRSMGKDCMQAAADGTTAYNSEGFVAAAQMLQ
                     DMNQKGYFGVGATTVDQNTAADMVLSGEAAMIYNGSWYTSSLNADTNPAGPDGIGFFN
                     IPTVDGGEGTITEYAMNCGTILCLNKSTYDDTMAGFLKYFVSNVGDYSMNEFGALKGY
                     VIENYPEDMSDYTKLVADEMGKVTGTANWFEALMNGELSTMAQENVQSLMNGDMSAQE
                     YCDAMQDCYESTK"
BASE COUNT          331 a          354 c          255 g          395 t
ORIGIN      
        1 tttttattac ccaaaacttt tttcaaaacc atccacctct ctattgttta ataggggcac
       61 acaggaatca ccctgcctgc ccctaacctg tgtctattat tcagttatca gctgtttatt
      121 ttgttgattc ataacaatcc tgcatcgcat cacagtattc ctgagcgctc atgtccccgt
      181 tcataagaga ctgtacattt tcctgcgcca tcgtggataa ttctccgttc atcaatgctt
      241 caaaccagtt ggctgttccg gtaaccttac ccatttcatc cgcaacgagc tttgtatagt
      301 cgctcatgtc ttccggataa ttttcaatga catatccttt cagtgcgcca aactcattca
      361 tggaataatc gccgacattt gatacaaaat atttcaaaaa tccggccatt gtatcatcat
      421 aggtgctctt gttcagacag aggatcgttc cgcagttcat tgcatattct gtgatcgtac
      481 cttctccgcc gtcaacagtc ggaatattga aaaaaccgat tccatcagga cctgccggat
      541 ttgtatccgc attcagggaa gaggtatacc agcttccgtt atagatcatt gctgcttctc
      601 cggataatac catatcagct gccgtattct gatcgactgt cgtagcgccg acaccgaaat
      661 aacccttctg attcatatcc tgaagcatct gtgcggctgc aacaaagcct tcactgttat
      721 atgccgtcgt accatctgct gcagcctgca tgcagtcctt gcccatagag cggatcgcat
      781 acgcatggat cagtcttgtt gccggccatg aatctcctgc gcctgttgca aacggctgga
      841 tccctgctgc cagaagcaga tctgcatcag caagcatttc atcccatgtc gtcggtgcct
      901 ctgtaattcc tgcctgctca aacagtgctt tgttatacca gaagccttca atattaaggc
      961 ctaacggcag atcgtaaata tcatcggttc ctgtaagtgc tgccagatac gtagttgctc
     1021 cgttatcaag cttgtcatag acacctgcat ccgtaagtgc ctgtgtaaca tccagcacca
     1081 gatcggcatc gatcaggtca acaagcggcg ttcctgcatc ataggcaaag atatccggaa
     1141 gatcatcgga agctgcaagt gtctgtactt ttgtctttaa attgtcggaa tcgacatact
     1201 caaattccca tgtaaaattc ggatccacat cagccttata ctgctcacac atcgccgtaa
     1261 taatacttcc gtttgctgtt gtcgatgaat cgtcctcagg ccacatggac aaaattttaa
     1321 ttgttctgga ttctg
//
LOCUS       DAOSYE010000753         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_92369_length_1330_cov_3.423529, whole genome shotgun sequence.
ACCESSION   DAOSYE010000753 DAOSYE010000000
VERSION     DAOSYE010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92369_length_1330_cov_3.423529"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLU43_11240"
     CDS             complement(<1..432)
                     /locus_tag="PLU43_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO29028.1"
                     /translation="MDSMRIAILGAGYIATIMATTLQPLKGAECYAVAARDIKRAQIF
                     ADKFGFKKAYGSYSEMLADEEVELVYIATPHSHHYEHIKLCLEHGKHVLCEKAFTANA
                     VQAEEVLRLAESKGLLLTEAIWTRYMPMRQTIDDIVKSGIIG"
     gene            complement(437..>1330)
                     /locus_tag="PLU43_11245"
     CDS             complement(437..>1330)
                     /locus_tag="PLU43_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOO29029.1"
                     /translation="REKRLAVLVDSENISPKYIKYILDEISNYGIATYKRAYGDWTSP
                     VSSAWKEVVLKNSITPMQQYGYTQGKNSTDSAMIIDAMDILYSGKVDGFCLVSSDSDF
                     TKLASRLREAGMLVIGMGEKKTVEAFRTSCTLFKYLEVLAAEEEGQSNDVSREDIEET
                     ILKIISDNEANDKDTTVGEIGNKISNRHADFDVRNYGYSKLSKFLESFKSIKLEQKGK
                     SIHVYLTESEVTKDQIEKIVGEIVRKAKDRTMSLPELKQKIEKKYPGFNIRDYKYSKF
                     SQFIKDMDGMSVRNNTVEQKE"
BASE COUNT          324 a          356 c          222 g          428 t
ORIGIN      
        1 gccgataata ccgcttttca caatgtcgtc gatcgtctga cgcatcggca tgtacctcgt
       61 ccagatcgct tctgttaaaa gaagaccctt tgattcggca agtcgtaaaa cttcctccgc
      121 ctgcactgca ttcgccgtaa atgccttttc acataaaaca tgcttcccgt gttcaaggca
      181 aagcttaata tgctcataat gatgggaatg gggcgttgct atatatacaa gctctacctc
      241 ttcgtctgca agcatttcag aataggaacc atacgctttt ttgaacccga atttatcggc
      301 aaagatctgt gcccgcttga tatctcttgc agccactgca tagcactccg cacctttgag
      361 cggctgcagc gttgttgcca ttatcgttgc tatataacca gctcccaata tcgctattct
      421 catactgtcc acatccttat tctttttgtt ctaccgtatt gtttcttacg gacatgccat
      481 ccatatcttt aataaactgg gaaaacttac tgtacttata atccctgata ttaaagccag
      541 ggtatttctt ctcaatcttc tgtttcagct ccggaagact cattgttctg tcctttgcct
      601 tccttacgat ctccccgacg atcttttcta tctgatcctt cgtcacttca ctttcagtca
      661 gatagacatg gatcgatttc cccttctgtt caagcttgat gcttttaaag ctctcaagaa
      721 atttagataa tttggaatat ccgtaattcc tgacatcgaa atccgcatgt ctgttactga
      781 tcttattgcc gatctccccg accgtcgtat ccttgtcatt cgcctcatta tcagaaatga
      841 tcttcaggat cgtctcttca atatcctccc tgctcacatc attggactgt ccttcctctt
      901 ctgctgcaag cacctccaga tatttaaaca gcgtacagga agtacgaaat gcctcaactg
      961 tctttttctc gcccatacca attaccagca taccggcttc cctcagcctt gaagcaagct
     1021 ttgtaaaatc actgtcgctt gatacaaggc agaaaccatc tactttgccc gaatacaaaa
     1081 tgtccatcgc atctatgatc attgccgaat ccgtagaatt ctttccctgt gtatacccgt
     1141 actgctgcat cggagtaatg gaatttttca gtacgacctc tttccatgct gaagaaaccg
     1201 gacttgtcca gtctccataa gcccttttat aagtggcaat tccgtaattt gatatttcat
     1261 ccagaatata tttgatatac ttgggggaaa tattttccga gtcgaccaat actgccagcc
     1321 gcttctctct
//
LOCUS       DAOSYE010000754         1324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_93091_length_1324_cov_2.350670, whole genome shotgun sequence.
ACCESSION   DAOSYE010000754 DAOSYE010000000
VERSION     DAOSYE010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93091_length_1324_cov_2.350670"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1324)
                     /gene="glgB"
                     /locus_tag="PLU43_11250"
     CDS             complement(<1..>1324)
                     /gene="glgB"
                     /locus_tag="PLU43_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOO29030.1"
                     /translation="VMEHPFDGSWGYQVIGYYAPTSRYGTPDDFMYFMDYMHKEGIGV
                     ILDWVPAHFPRDVQGLSNFDGTCLYEHKDPRQGFHPHWGTLLYNYGRAQVQNYLIANA
                     LFWINQYHADGIRIDAVASMLYLDYGKNDGEWVANIYGGNENLEAVELLKHLNSINKK
                     QNTGALMIAEESTAWPQVTGDLAKGGLGFDYKWNMGWMNDFLDFMHYDPYFRAHHYGE
                     LTFSMIYAYSEKFMLVFSHDEVVHGKASMIGKMPGDREEQFANLRAAYGYMMMHPGKK
                     LSFMGQDLAEFDEWNEMRSVEWELLQYQEHRQFHEYIKELLKLYRTHPAFYQSDDHPD
                     GFEWINNISANETIIVFLRKSEDGETMLVVFNFANAAREQYKIGVPYTGKYKEIFNSD
                     AKKFGGTGFVNPRVKSSKKDECDGREDSIKIKVAPLSVSIFQCTFEQPT"
BASE COUNT          358 a          347 c          242 g          377 t
ORIGIN      
        1 cgtgggctgt tcaaaagtac actgaaaaat actgacggat agcggcgcga cttttatctt
       61 tatcgaatcc tcacggccgt cgcattcatc ctttttggat gatttcacac gcgggttgac
      121 aaatcctgtg ccgccgaatt ttttcgcatc cgaattaaag atctctttat atttacccgt
      181 ataaggaacc ccgatcttat actgctcccg tgctgcgttt gcaaaattaa agacaacaag
      241 catggtttcg ccgtcttctg actttcttaa aaaaacaatg atcgtttcat tggcggaaat
      301 attattgatc cattcaaagc cgtccggatg gtcgtcggac tgataaaatg ccggatgcgt
      361 tctgtatagt ttcagaagct cttttatata ctcatgaaac tgcctgtgct cctgatattg
      421 aagaagctcc cactccacgc tcctcatttc gttccattca tcaaactcgg caagatcctg
      481 ccccataaac gacagttttt tcccgggatg catcatcata taaccgtaag ccgctcttaa
      541 atttgcaaat tgttcctccc tgtcacccgg cattttcccg atcatggatg cctttccatg
      601 tacgacctca tcatgtgaaa atacaagcat aaatttctca ctgtaagcat agatcatgct
      661 gaatgtaagc tctccataat gatgcgccct gaaataggga tcgtaatgca tgaaatcaag
      721 aaaatcattc atccagccca tattccactt ataatcaaag cccagaccgc cttttgcaag
      781 atctcccgtc acctgcggcc atgcggtcga ctcctcagca atcatcagtg cacccgtatt
      841 ctgttttttg ttgatggaat ttaaatgctt cagtaactca acagcttcca gattttcatt
      901 gccgccgtaa atattggcta cccattcccc gtcatttttt ccataatcca gatacagcat
      961 ggatgctacc gcatctatgc ggataccatc cgcatgatac tgattgatcc aaaacagtgc
     1021 atttgcgatc agatagtttt ggacctgtgc tcttccgtaa ttataaagca gtgtacccca
     1081 atgcggatgg aaaccctgcc tcggatcctt gtgctcatac agacaggttc cgtcaaaatt
     1141 ggataatccc tggacatctc tcggaaaatg cgcaggcacc cagtcgagaa taacgccgat
     1201 cccttcttta tgcatataat ccataaaata cataaaatca tcaggtgttc catagcggct
     1261 cgtcggtgca taataaccga tgacctgata cccccaggac ccgtcaaacg gatgctccat
     1321 aaca
//
LOCUS       DAOSYE010000755         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_93921_length_1317_cov_3.797940, whole genome shotgun sequence.
ACCESSION   DAOSYE010000755 DAOSYE010000000
VERSION     DAOSYE010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93921_length_1317_cov_3.797940"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLU43_11255"
     CDS             <1..930
                     /locus_tag="PLU43_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOO29031.1"
                     /translation="GAGKNAKRAAVIHLMFNVIGAGLTGVLMFIVFSVEKEFASSYIS
                     SVQISIFHTIFNVTNTLVLLPFANLLVKLSGMIVKGEDEKEKEDEAVAALRHMDERIL
                     ESPSFAVENVKKEVVHMGRFALEHFKIAMSALCEKNTKKIKKVRRMEETMDKLAELIT
                     EYLVKISNLSLTEEQKKVVNYMFNAVTDLERIGDHCDNLADLAEKYEKMDVEFSDKAR
                     KGLDKIYNKTFEAVSHALDALEFSDKSEVALTEACEQAVDALEEELRKGHMKRLSNKT
                     CNAAKGIIFLDALTNIERISDHAMNVAEYVLEN"
     gene            1050..>1317
                     /locus_tag="PLU43_11260"
     CDS             1050..>1317
                     /locus_tag="PLU43_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29032.1"
                     /translation="MAYLEDTDRPSFLSQMIIDTAELQWIQDIYAKLNNLFIFSVNKD
                     GKRITEISGNPGEIERIFGLVEERQIVSVLQRVLGSSVEEQVVED"
BASE COUNT          426 a          214 c          336 g          341 t
ORIGIN      
        1 ggtgcaggaa agaatgcaaa gagggcggct gtcatccatc tgatgttcaa tgtgatcgga
       61 gccggactga caggtgtgct gatgtttatt gtcttttcag tggaaaagga atttgcatca
      121 agttatatca gcagtgtgca gatctctatt ttccatacga tctttaatgt tacgaatacg
      181 cttgtacttc tgccgtttgc gaatctgctt gttaaattgt ccggcatgat cgtgaagggc
      241 gaagatgaaa aggaaaaaga ggacgaagct gttgccgcac tgagacacat ggacgaaaga
      301 attttggaat caccgtcatt tgccgttgag aatgtaaaaa aagaagtggt gcatatgggc
      361 agatttgcac ttgagcattt taagatcgca atgtccgcgc tgtgtgaaaa aaatacaaag
      421 aagatcaaaa aagtccgccg tatggaagaa acaatggata agctggcgga actgatcacg
      481 gaatatcttg tgaagatcag taatctgtca ctgacggagg aacagaaaaa ggtcgtcaat
      541 tatatgttca atgcggtcac ggatctggaa cggatcgggg atcactgcga taatcttgcc
      601 gatcttgcgg aaaaatacga gaaaatggac gtggagtttt cagataaggc aagaaaaggc
      661 ctggacaaaa tatacaataa aacatttgaa gcggtatctc atgctctgga tgcgctggaa
      721 ttttcagaca agagcgaggt ggcactgaca gaggcctgcg aacaggctgt ggatgcactg
      781 gaagaagagc tgcgcaaggg ccatatgaag cggctgtcca acaaaacgtg caatgctgca
      841 aaggggatca ttttccttga cgcgcttaca aatatagagc gtatatccga tcatgcgatg
      901 aatgttgctg aatatgtact ggaaaactaa agtgtctttt gtgaaaagtg tataaaattt
      961 cacagagatt ttattgcaaa tgcagttgaa aaaaaagaca atttcgtgta tgctagtagt
     1021 atcgtattaa agtgtgaaag gatgtcacta tggcttatct ggaagacaca gaccgtcctt
     1081 cttttttgtc gcaaatgata atagacacag cagaacttca atggatacag gatatttatg
     1141 caaagcttaa taatttgttc attttcagtg taaacaaaga tgggaaaaga atcactgaaa
     1201 tatccgggaa tcccggagaa atcgaaagaa tttttggact tgtagaagaa agacagattg
     1261 tttctgttct tcagcgtgta ttaggaagct ctgtggagga acaggtcgta gaggata
//
LOCUS       DAOSYE010000756         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_94193_length_1315_cov_3.261111, whole genome shotgun sequence.
ACCESSION   DAOSYE010000756 DAOSYE010000000
VERSION     DAOSYE010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94193_length_1315_cov_3.261111"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLU43_11265"
     CDS             complement(<1..803)
                     /locus_tag="PLU43_11265"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOO29033.1"
                     /translation="MEFGTGSNVAIIVLWNYFSILVNVVAFVIIYMKAKRSPSLKAFF
                     LVQLSMLLWLVGKVLKTVSPNEELRWFFIVFYYAGICLLEVSFFDFAYIYGKNKPLKK
                     PFRIAVYLLGFLQFMTVVTNPYHHLFYSVYGFWEDEFGALFYVHVVINYGFMIAAMIL
                     AGRKFKIQLSDKTRAEKNLISLAILVPLVLNFVYITRMLESLFRILHIQIFDITPIVY
                     TWSNLIFVYATYKYEFFDLTPIMKHEIARKLDTPVLILNRERALLYANE"
     gene            complement(833..>1315)
                     /locus_tag="PLU43_11270"
     CDS             complement(833..>1315)
                     /locus_tag="PLU43_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO29034.1"
                     /translation="LMDIEMPQMDGIEATKIIKTRFPDMKVIILTTFENPDNIMESFV
                     NDADGYIVKNISYKDLKRTIKCVNNGLTVIHESVRKIMVDRFRGLSEYKARYKDVLTD
                     REAEIVKYIAAGLSNKEIAAKLSYSPGTIKNDVSKILEKLDMADRMQIAVFAIENGMV
                     "
BASE COUNT          411 a          309 c          229 g          366 t
ORIGIN      
        1 tgttcattgg catacagcaa cgcacgctca cgattcaata ttaaaacggg cgtgtccagt
       61 tttctggcaa tttcatgctt cataatggga gttaaatcaa aaaattcgta tttataagtt
      121 gcatacacaa aaataaggtt cgaccaggtg tacacgatcg gggtaatgtc aaagatctga
      181 atatgcagta tgcgaaacag cgattccagc atgcgggtga tatagacaaa attcaataca
      241 agcggcacaa gtatggccag tgaaatgaga ttcttttcag cccttgtctt gtcgcttaac
      301 tggatcttga atttcctgcc cgccaggatc atggcagcaa tcataaatcc atagttgatt
      361 acaacatgaa cataaaacag agcgccaaac tcatcctccc aaaagccata cacgctgtag
      421 aacagatgat gatacgggtt cgtcactaca gtcataaact gcagaaatcc caaaagatag
      481 accgcaatcc tgaacggttt cttaagaggt ttgtttttac cgtagatata ggcaaaatca
      541 aaaaaactga cctccagcag acagatgccc gcataataaa acacaataaa gaaccagcgc
      601 agttcttcgt ttggggaaac tgttttaagc acctttccga ccagccagag cagcatggat
      661 aactgtacaa gaaaaaatgc ctttaaagaa gggcttcgtt tggctttcat ataaatgatc
      721 acaaaagcaa caacattgac cagtatggaa aaataattcc ataaaacaat gattgccaca
      781 ttactgcctg taccaaactc catactacac ttccatgccc cctgttacgg tgctaaacca
      841 taccgttttc tattgcaaaa acagcaatct gcatccgatc cgccatatca agcttctcaa
      901 gtattttgct cacatcattt ttgatcgtac cgggcgaata cgaaagcttt gccgcgatct
      961 ctttattgct cagccctgct gcaatatact ttacgatctc cgcctccctg tcggtcagaa
     1021 catctttata tctggctttg tattcgctaa gtcccctgaa acgatcgacc attatttttc
     1081 tgacgctctc atggatcacc gtcagcccgt tattcacaca cttgatcgta cgctttaaat
     1141 ctttataaga aatatttttc acaatatagc cgtccgcatc attgacaaaa gactccataa
     1201 tattgtcagg attttcaaat gtagtcaata tgatcacttt catgtccgga aacctggtct
     1261 ttataatttt tgtagcctca ataccatcca tttggggcat ctcgatatcc attaa
//
LOCUS       DAOSYE010000757         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_94196_length_1315_cov_3.200794, whole genome shotgun sequence.
ACCESSION   DAOSYE010000757 DAOSYE010000000
VERSION     DAOSYE010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94196_length_1315_cov_3.200794"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLU43_11275"
     CDS             complement(<1..362)
                     /locus_tag="PLU43_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOO29035.1"
                     /translation="METLTQYIDKLKKLNFKNMYQNDFFLTWEKTDDEIEAVFLVADA
                     LRYMRKNNISTKVFESGLGISLFRDNSTRTRFSYASACNLLGLEVQDLDEGKSQIAHG
                     ETVRETANMISFMADVIG"
     gene            complement(376..>1315)
                     /locus_tag="PLU43_11280"
     CDS             complement(376..>1315)
                     /locus_tag="PLU43_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOO29036.1"
                     /translation="WEFDPYEGYENETEIGGRGTSDQLGGIVSSVYGAKIMKDLGLLN
                     DTYTVLVTGTVQEEDCDGLCWQYIINEDQIKPEFVVITEPTDGNIYRGQRGRMEIRVE
                     VQGISCHGSAPERGDNAIYKMADILQEVRALNDKLLDNDFLGKGTLAVSEIFFTSPSR
                     CAVADMCAISIDRRLTDGETYQSALDEIAALSSVKKYDAAVTMYQYERPSYTDLVYPT
                     DCYFPTWVIPEEHPATKAMADAYKGMFGEPVIDKWTFSTNGVSIMGRYGIPCIGFGPG
                     REAQAHAPNERTWKADLVKCAAVYAALPSLYTKQES"
BASE COUNT          316 a          317 c          310 g          372 t
ORIGIN      
        1 ataccgatca cgtctgccat aaaggagatc atattggctg tttcccggac tgtttcacca
       61 tgtgcgatct gagattttcc ttcgtccaga tcctgtactt ccagacctaa cagattgcag
      121 gccgaagcat aggaaaagcg cgtgcgtgtt gaattatcgc ggaacagtga aatgccgagt
      181 ccgctttcga atacttttgt ggaaatatta tttttgcgca tatagcggag tgcatccgcc
      241 acgagaaaga cggcttcgat ctcatcatct gttttttccc atgtgagaaa aaaatcgttt
      301 tggtacatat ttttaaaatt cagtttcttc agcttgtcaa tatattgggt taaagtttcc
      361 attggtccta tcctcctatg attcctgttt tgtgtacaga cttggcagag cggcataaac
      421 agcggcgcat tttacaagat ctgctttcca ggtgcgttcg tttggcgcgt gtgcctgtgc
      481 ttccctgccg ggaccaaagc cgatgcacgg aataccatag cggcccatga tcgaaacacc
      541 gtttgtggaa aacgtccatt tatctatgac aggttcaccg aacattcctt tataggcgtc
      601 tgccattgct tttgtggcag gatgctcttc cgggatgacc catgtcggga aatagcagtc
      661 cgtagggtat acaagatccg tatagcttgg acgctcgtat tgatacatgg ttacggcagc
      721 atcatatttt tttacggacg aaagtgctgc gatctcgtcg agtgcgctct gataagtttc
      781 tccgtctgtc agacggcggt caatggagat cgcacacatg tccgcaactg cgcatctgga
      841 aggagaagta aagaagatct cggatacggc aagtgttccc tttccgagaa aatcgttgtc
      901 aagaagctta tcattaagag cccgtacttc ctgcagaata tcggccattt tgtaaatggc
      961 gttgtcaccg cgctccggtg cggagccgtg acaggaaatg ccctgtactt caacgcgaat
     1021 ttccatacgg ccgcgctgac cacggtaaat attgccgtcg gtcggttccg ttatgacaac
     1081 gaattccggt ttgatctgat cttcattaat aatgtactgc cagcagagac cgtcgcagtc
     1141 ttcctcctgt accgtacctg tgacgagaac ggtataggta tcatttaata atccaagatc
     1201 cttcatgatc ttagccccat atacggagga aacaataccg ccgagctgat ctgatgtacc
     1261 gcggccgccg atttccgttt cgttttcgta gccttcatac ggatcaaatt cccag
//
LOCUS       DAOSYE010000758         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_94230_length_1315_cov_2.418254, whole genome shotgun sequence.
ACCESSION   DAOSYE010000758 DAOSYE010000000
VERSION     DAOSYE010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94230_length_1315_cov_2.418254"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLU43_11285"
     CDS             complement(<1..666)
                     /locus_tag="PLU43_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOO29037.1"
                     /translation="MIKDNQRVFNRLHVIIDGMVVACSYIFAWFLKFRSGLLEADLGL
                     PMQTYFMALYFIVPGYLFLYYQFDLYNSKRASGRKRELFNIIKANMLGLIAFMVVLYV
                     INQPHFSREMIFVFGAFNIFAETFVRNVIRYMLRFIRRRGYNLKYVLLVGYSKSAESY
                     INKIRLNPQWGYVVRGILDDHVERGTLYKGVKVLGRIDNLFVILPENKLDEIAITLSL
                     AEYG"
     gene            complement(807..>1315)
                     /locus_tag="PLU43_11290"
     CDS             complement(807..>1315)
                     /locus_tag="PLU43_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO29038.1"
                     /translation="YEKETTVFSACAGAAIYRRELFEQIGYFDEFHFSYLEDVDVGYR
                     ARIMGYKNRFTPKAVVYHAGSGTTGSRYNAFKIRLASRNSWYVIYKNMPAGQIILNLP
                     FFIIGFGIKTLFFICKGYGREYLSGMKRGYLMCTQGKKYPYSARFLKNYARIQLELWL
                     NLFRRIFG"
BASE COUNT          414 a          323 c          217 g          361 t
ORIGIN      
        1 gccatattcg gccagactca gcgtgatcgc gatctcgtcc agtttatttt caggaaggat
       61 cacaaacaga ttatcaatcc gcccgagaac cttgacgcct ttatacagcg ttcccctttc
      121 cacatggtca tccagaatac ctctgacaac atatccccac tgcggattca gtctgatctt
      181 attaatatag gattccgccg attttgagta accgacaagc agcacatact tcaaattata
      241 tccgcgcctt ctgataaagc gcagcatata acggatcaca tttctgacaa acgtttccgc
      301 aaaaatatta aacgccccga aaacaaagat catttcacgg gaaaaatgcg gctggttgat
      361 cacgtaaagt acaaccatga atgcgatcaa tccgagcata tttgccttga taatattgaa
      421 taattcccgt ttcctgccag atgcacgttt ggaattgtac agatcaaact gataatataa
      481 aaacaaatag ccgggtacga taaaataaag cgccataaaa taagtctgca tcggaagacc
      541 cagatccgct tctaacaatc cgctcctaaa tttcaaaaac catgcaaata tataggaaca
      601 ggcaactacc attccgtcaa taattacatg aagtctgtta aatacccgct gattatcctt
      661 gatcatactg tttcaccgtt cgggaatgtg tctttgctgc tattctgtca cgcagtgacc
      721 tccccgtcaa ttatttatta aatatacaat agtttatata aaagggcaac caaaataccc
      781 gcttttattt attaaaaatc tcttaactag ccgaaaatcc tgcgaaacag attcagccac
      841 agctccagct gaatacgtgc gtaatttttt aaaaatctgg ccgaatacgg atattttttc
      901 ccttgtgtac acatcagata tcctctcttc ataccggaaa gatattccct gccatagcct
      961 ttgcatataa aaaacagcgt cttgatacca aatccaatga taaagaacgg caaattcaaa
     1021 atgatctgcc ctgcgggcat gtttttataa ataacgtacc agctgtttct ggacgcaagt
     1081 ctgatcttaa aagcattata gcgcgagcct gttgttccgc ttcccgcatg atacacaacg
     1141 gctttcggtg tgaatctgtt tttatatccc atgatcctgg cccgataccc gacatcaaca
     1201 tcctccagat aggaaaaatg aaattcatca aaataaccaa tttgttcaaa cagctccctg
     1261 cggtaaatcg ccgcacccgc acacgccgaa aatacagttg tttctttctc gtaat
//
LOCUS       DAOSYE010000759         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_94749_length_1311_cov_2.675159, whole genome shotgun sequence.
ACCESSION   DAOSYE010000759 DAOSYE010000000
VERSION     DAOSYE010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94749_length_1311_cov_2.675159"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..811)
                     /locus_tag="PLU43_11295"
     CDS             complement(23..811)
                     /locus_tag="PLU43_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO29039.1"
                     /translation="MTKKVLQNLFIVLVFLFLYIPIFMVIVFSFNTSKINIVWEGFTI
                     QWYGSFFQNRTLMESLWISLLIAVMSTAISTVIGTLGALGLSKFNFKGKDLIDQLLYI
                     PVVIPEVVLGVALLCIYSTLNMDLGIGTITLSHVTFCIPFVVINVRARLAGFDKCLEE
                     AAMDLGANPVLTFFKITLPLLMPGVAAGALLSFSLSLDDVIISFFVTGPGSTTLPLKI
                     LSMVKTGVTPEVNALSTIIMFTVMIIISINTGAKVKQLRKDTVR"
     gene            complement(813..>1311)
                     /locus_tag="PLU43_11300"
     CDS             complement(813..>1311)
                     /locus_tag="PLU43_11300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29040.1"
                     /translation="WINLLRREGIVNRVLMAIGLIKEPLQLVYNNTGAIIGLVYTLLP
                     FMIMPLYSSIEKLDKSLLEACSDLGAKPAVAFWKVTLPLTMPGIFAGTIMTFIPTLGL
                     FFVIDLMGGSKVLVMGSLIRNQFITAKNWPLGAALSLFLIAITLLLVKIYQHNGGNMD
                     ELGGV"
BASE COUNT          395 a          325 c          266 g          325 t
ORIGIN      
        1 taataacaag ttcaaacatc agctatctca ccgtatcctt tctcagctgc tttacctttg
       61 ctcctgtatt gatactgata ataatcatga ccgtaaacat aatgatcgtt gaaagcgcat
      121 ttacttccgg cgtgactcct gttttaacca tggaaaggat cttgagcggc aatgtcgtgc
      181 tgcctggccc tgttacgaaa aagctgatga ttacatcatc taaggacaag gaaaacgaca
      241 gcagggcacc ggcagccaca cccggcataa gcagcggaag tgtgatctta aaaaaagtca
      301 gaacagggtt ggctccaaga tccattgccg cttcttccag gcatttgtca aatccggcca
      361 gtctggcccg tacattgatc accacaaacg gaatacaaaa tgtaacatgt gagagcgtga
      421 tcgtcccgat cccaagatcc atgttcagcg tggaataaat gcagagaagc gccacaccca
      481 gtacaacttc cggaataacg accggtatat ataaaagctg atcgatcaga tcttttcctt
      541 taaaattgaa tttactaagt ccgagggccc caagcgtacc gatcactgtc gagatcgccg
      601 tgctcatcac tgcgatcaga agactgatcc agagtgattc catcagcgtc ctgttttgaa
      661 aaaagcttcc gtaccattgt atcgtaaagc cttcccatac gatattgatc ttagatgtat
      721 taaaagaaaa aacaataacc atgaagatcg gaatatataa aaacagaaaa acaagcacaa
      781 taaacagatt ctgcaatact ttcttcgtca ttctatacac ctcccagttc gtccatatta
      841 ccgccgttat gctgatatat cttgaccaaa agcagcgtta tggcaatcag gaacagggaa
      901 agcgccgccc caaggggcca gttcttggcg gtaataaact gatttctgat cagacttccc
      961 ataacaagca ctttgcttcc gcccatcagg tctatgacaa aaaacaatcc aagtgtcgga
     1021 ataaacgtca tgatcgttcc ggcaaaaata cctggcatgg tcagaggcag cgttactttc
     1081 cagaatgcta ccgcaggctt tgctccaaga tcactgcagg cttccagcag tgatttgtcc
     1141 agtttttcaa tggaactgta cagcggcatg atcataaaag gaagcagtgt atagaccagt
     1201 ccgatgatcg cacccgtatt attatataca agctgcagcg gctctttgat caggccgatc
     1261 gccatcaaga ccctgtttac gattccttcc ctgcgcagca gattgatcca t
//
LOCUS       DAOSYE010000760         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_94885_length_1310_cov_2.455777, whole genome shotgun sequence.
ACCESSION   DAOSYE010000760 DAOSYE010000000
VERSION     DAOSYE010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94885_length_1310_cov_2.455777"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..501
                     /gene="fliS"
                     /locus_tag="PLU43_11305"
     CDS             127..501
                     /gene="fliS"
                     /locus_tag="PLU43_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOO29041.1"
                     /translation="MMSNAYAQYQTNKILTASPAELTLMLYDGAIKFCNVAIMGIEQG
                     NIQKTHDNIMKTQKIIEEFQITLNFDYPIANDFNNVYNYLIKRLREANAKKDRSILVE
                     VLEHLHTMRDTWKEVMRLAHAS"
     gene            551..1045
                     /locus_tag="PLU43_11310"
     CDS             551..1045
                     /locus_tag="PLU43_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HOO29042.1"
                     /translation="MADSKKYIAIMLESLHKKEALLDLLLLKNEAQAACIRNKEYDDV
                     KWASFHVLMAEKQSAIDRLNKLDDGFETLYARVREELQENKAEYEEEIRLMQELITRI
                     TDKGVAICTGEENNRRMIEKILGNTRKEIRQVRKSLQVASSYYKSMRKISPESTSQFM
                     DKKK"
BASE COUNT          451 a          216 c          326 g          317 t
ORIGIN      
        1 tttgccgata tggaaacagc gttaagtacg ctgacttccg aacagtctgc ttttgagagt
       61 ctgcttggca gtttataagg gatataaata acaggggttg atcaaggagg atagaccatg
      121 gctacgatga tgagcaatgc ttacgcacag taccagacaa ataaaatttt gacagcttca
      181 ccggcggaat tgacattgat gctgtatgac ggagcaatca agttttgcaa tgttgcaatt
      241 atgggtattg agcagggaaa tatccagaaa acacatgata atattatgaa gacacaaaag
      301 attattgaag agtttcagat aacgcttaat tttgattatc ctattgcgaa tgattttaat
      361 aatgtatata attatcttat taaaagactt cgcgaggcaa atgccaaaaa ggacagatcg
      421 attcttgtgg aagtgttgga gcatctgcat acaatgcgcg atacatggaa agaagttatg
      481 cggctggcac atgcgtcctg atcctgtcgg cagttttgcc ggacttgcgg ttatctgaat
      541 aggagaagag atggcggaca gtaaaaagta tattgcgatc atgctggaaa gtctgcataa
      601 aaaggaagcg ttgctggatc tgcttctttt aaaaaatgag gcacaggcag cgtgtatcag
      661 gaacaaggaa tatgacgatg tcaaatgggc gtcttttcat gtactgatgg cggaaaaaca
      721 gtcggcgatc gacaggctga ataaactgga tgacgggttt gaaacgcttt atgcaagggt
      781 aagggaagaa ctgcaggaaa acaaagcaga atatgaagaa gaaatccggc tgatgcagga
      841 actgatcacc cgtattactg ataaaggcgt agcgatctgt acgggcgaag aaaataaccg
      901 ccgcatgata gaaaagatac tcggcaatac aaggaaagag atccggcagg tcagaaagag
      961 tctgcaggtc gcaagcagtt attataaaag catgagaaaa atatctccgg aatcaacttc
     1021 tcagtttatg gataaaaaga aatagattta aaaagataat attccggtat aaagtttttg
     1081 gtttttatac cgatatatta tacaagtaaa ggaacttact tacgagtgca aagaaaccgg
     1141 aaggagcgta cggccggaaa ggatcagagc gaagttataa gtacctgcta acaactatgg
     1201 cggcatggaa gccgcgacac tttcaaggag gaatgattta tggtagtaca acacaatatg
     1261 acagcgatga acgctaacag gcagcttggc attacaacat ctaaccaggc
//
LOCUS       DAOSYE010000761         1309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_95100_length_1309_cov_1.618820, whole genome shotgun sequence.
ACCESSION   DAOSYE010000761 DAOSYE010000000
VERSION     DAOSYE010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95100_length_1309_cov_1.618820"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>233)
                     /locus_tag="PLU43_11315"
     CDS             complement(<1..>233)
                     /locus_tag="PLU43_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29043.1"
                     /translation="QMGLFIDGDGIPLAFSTFPGNQNEQTSLKPLESKILQQFGCDKF
                     IYCSDAGLASQGNRNFNHMGKRSFIVTQSIKKLR"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<244..924)
                     /locus_tag="PLU43_11320"
     CDS             complement(<244..924)
                     /locus_tag="PLU43_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO29044.1"
                     /translation="MRLHTSKSKNAESFYISKGYVNDKGVSTSVIVRKLGTLAELLPE
                     HGPTRDDVVAWCKEQCRVETMKYKEEQETKSVQITFHADRPLEYSQQRFYKGGYLFPQ
                     SVYYGLQLDKICRKIKAKHKYEFDLNAILSDLIYNRVLEPQSKLSAYKTAQSFLEPPT
                     YKLHDVYRALSILAEENDLIQSEVFKNSNFLGERNSRILYYDCSNFYFEIEQEEGDKK
                     YGKSKEHRP"
     gene            1066..>1309
                     /locus_tag="PLU43_11325"
     CDS             1066..>1309
                     /locus_tag="PLU43_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29045.1"
                     /translation="MVTNTIQYGFKDQKEHFIDIKVQAIGEGAKLRIRDDGAAFNPMD
                     YLKEQDSDDLIHNIGIRMVKEIAKEVDYRNTMRMNNL"
BASE COUNT          379 a          262 c          274 g          384 t
ORIGIN      
        1 cggagcttct tgatggactg ggtcacaata aaggaacgtt ttcccatgtg gttgaagttc
       61 ctattaccct gagaagccag ccctgcatcg ctgcagtata taaatttatc acatccgaac
      121 tgctgaagga ttttagactc taatggtttt aaagaagtct gttcattctg attaccgggg
      181 aaagtagaaa atgcaagtgg aataccatcc ccatcaatga acagtcccat ctgnnnnnnn
      241 nnnaggtctg tgctctttgc tttttccata cttcttgtcg ccttcctcct gctcgatttc
      301 aaaataaaag ttagagcagt cgtagtatag aattctggaa ttcctttcgc ctaaaaagtt
      361 actgttctta aatacttcgg attggatcag atcgttttct tctgcaagaa tgctgagagc
      421 acggtatacg tcgtgaagtt tgtaagtagg cggctccagg aaactctgtg cagttttgta
      481 ggcggagagt ttactttgtg gctccagaac ccggttatag atgagatctg aaagaattgc
      541 atttaaatcg aattcatatt tatgtttggc tttgatcttg cggcatattt tatctaattg
      601 aagcccataa taaacagact gtggaaaaag atatcctcct ttataaaacc gctgctggct
      661 gtattcgaga ggtctgtcag catgaaaagt gatctgaacg gatttcgttt cctgttcttc
      721 tttatatttc atggtctcga cccggcactg ctccttacac caggcaacca catcatccct
      781 ggtagggcca tgctcgggca gaagctcggc tagtgtacca agtttgcgga caataacaga
      841 agtgctgacg cctttatcat taacatatcc cttactgatg taaaaagatt ctgcattttt
      901 agattttgaa gtgtgaagtc tcataagaaa atcctcctat accttattat acaacacatt
      961 acaataaagt acaatagtat aaaactataa aattgacgta aaaaaagcag gctttatgcg
     1021 acctgcgact actttttcct ttattcaact gtcaaactcc cggaaatggt tacgaatacg
     1081 atccaatatg gattcaagga tcaaaaggag cattttatcg atatcaaagt gcaggcaatc
     1141 ggtgagggag cgaagcttcg tatccgagac gacggcgctg cattcaatcc gatggattat
     1201 ttgaaggagc aggacagtga cgatctgata cacaatatcg gaatccgtat ggtaaaggaa
     1261 atcgccaagg aagtcgatta ccggaatact atgcgcatga ataatctgt
//
LOCUS       DAOSYE010000762         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_95753_length_1303_cov_2.129006, whole genome shotgun sequence.
ACCESSION   DAOSYE010000762 DAOSYE010000000
VERSION     DAOSYE010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95753_length_1303_cov_2.129006"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1303
                     /locus_tag="PLU43_11330"
     CDS             29..>1303
                     /locus_tag="PLU43_11330"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOO29046.1"
                     /translation="MAGFFTDEPRLTCNHFGDIAWSDDLPGEFQKQYGYDIRIVIPSL
                     YRKAGDYKKVRYDFWTLVSRMFSEHYMKTLFDWCEAHQCKLTGHIMMEESIFSQMTST
                     AGVMPFYEYMHIPGIDWLRRRIDSPVIGKQVGSVACQLGKKQVLTETYALCGWGVTFE
                     ELKWIAEWQFVNGVNQICQHLMAYTIKGSRKRDYPPSHFTQQTWWPEARSFHDYLARL
                     CAALSYGDQTADVLLLHPMRSGYIVYDGTRTEEIRELDEAFIKASNLLTEEQISYHYG
                     DETIIGKYGSVDGRCLKVGRIGYRAVILPQMYAIDKKTAALLLEFAKGGGLVISLDRL
                     PDYTNGDTKLLAGLLGHIRQVDYKSLRRTLEEQGLTGLRIHALQTEGKLPIAYQQREG
                     EEASIYFLVNHDQERTIETVIEFSGQTGELYRM"
BASE COUNT          369 a          250 c          358 g          326 t
ORIGIN      
        1 gcggttccaa aaatcgttcg gaaagacaat ggctggtttt tttaccgatg agccaagact
       61 cacctgtaat catttcggtg atattgcgtg gtcggatgat cttcccggag aatttcaaaa
      121 acagtacgga tatgatatca gaattgtgat tccttctctt tacagaaaag cgggagatta
      181 taaaaaagtc cgttatgatt tttggacact tgtcagcagg atgttttccg agcattatat
      241 gaaaacgctg tttgactggt gtgaagcgca tcagtgtaaa ttaacaggcc atattatgat
      301 ggaggagagc atattcagcc agatgacaag tacggccggc gtgatgccgt tttatgaata
      361 tatgcatatt ccgggaattg actggctgcg aaggagaatc gattctccgg tcattggaaa
      421 acaggtcgga agtgttgcct gtcagctcgg gaaaaagcag gttttgacag agacttatgc
      481 attgtgcggc tggggagtta cttttgaaga attaaaatgg atcgcagaat ggcagtttgt
      541 gaacggtgta aatcagatct gtcagcatct gatggcttat acgatcaaag gctccaggaa
      601 aagggattat ccgccgtctc attttacgca gcagacatgg tggccggagg cacggtcatt
      661 tcatgattat ctggccaggc tttgtgcggc gctgtcttat ggagatcaga cagccgatgt
      721 attgctctta catccaatgc gaagcggcta tattgtctat gacggaacaa ggacagaaga
      781 gatcagggaa ctggatgagg catttataaa agcgtcaaac ctgctgacgg aagaacagat
      841 cagctatcat tatggggatg aaacgataat cggcaaatac ggctcagtgg acggcagatg
      901 tctgaaagtt ggaagaatag ggtatcgtgc tgtaattctt ccgcagatgt atgcgatcga
      961 taaaaagaca gcagctcttc tgctggaatt tgcaaaaggc ggcggactcg tgattagtct
     1021 ggaccgtctg ccggactata caaatgggga tacaaaactg ctggcaggcc tgcttggcca
     1081 tatcaggcag gtggattata aaagcctgcg ccggacgctg gaagaacaag gactgaccgg
     1141 tctgcgcata cacgcactgc agacagaagg gaaactgccg atcgcgtatc agcagcggga
     1201 gggagaagaa gcctccatct attttcttgt caatcatgat caggaacgta cgattgaaac
     1261 tgtgatcgag ttttccggtc agacagggga attataccgc atg
//
LOCUS       DAOSYE010000763         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_96329_length_1298_cov_3.670153, whole genome shotgun sequence.
ACCESSION   DAOSYE010000763 DAOSYE010000000
VERSION     DAOSYE010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96329_length_1298_cov_3.670153"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLU43_11335"
     CDS             <1..640
                     /locus_tag="PLU43_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29047.1"
                     /translation="SVEHALMMIFYILLDIRDKKISSCSALQQYVYSDILQKLQQTLH
                     DISSDPLESFEERNELFLDLTENYRKEGLYTHDLKAAVSLAENHTVSYDSAHVLAQLA
                     AFHADMESFEHLFRNYLISELFSELLLPDSDEKDMIIMAEWIAMEYAAILHLLFLQAQ
                     MHKKMSDFSLIRDTIVIVSRMMGYEQEDIMEYMESCFQSPLWDWGYLALLVI"
     gene            704..>1298
                     /locus_tag="PLU43_11340"
     CDS             704..>1298
                     /locus_tag="PLU43_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29048.1"
                     /translation="MLSAIQKLFTTAVILTAAFLLSSCGTIENSSIGEPVSTVPEDTE
                     NLGTSATAQIPNPMVEITDASVFSDKLGITLDPDCLNENTTMYIIGERLAEIDYTVTN
                     VEGEAVSCTLRAAKATEHWDNLCGVYDEMTETTEEYACNETSITVIHKTPKTEPVEIY
                     EFTYQNIRYCFMYSGKLSNLLTNELINGVFCAIGAETV"
BASE COUNT          379 a          299 c          268 g          352 t
ORIGIN      
        1 ttccgtggag catgcactta tgatgatctt ctatatttta ctggatatcc gcgacaaaaa
       61 aatttcatcc tgcagcgctc tgcagcaata tgtatattcg gatattctgc aaaagctgca
      121 gcagactctg cacgatatat catccgaccc gcttgaaagc tttgaggagc gaaatgaatt
      181 atttctggat ctgactgaaa actaccggaa agaagggctt tacacacatg atctgaaagc
      241 tgccgtatcg cttgccgaaa accacacagt ttcctatgat tctgcgcacg tgcttgcaca
      301 gcttgcggca tttcatgccg acatggagtc ctttgaacac cttttcagaa attatctgat
      361 ctcagagctg ttttcagagc ttctgcttcc ggactctgat gaaaaagata tgattatcat
      421 ggctgagtgg atcgccatgg aatacgctgc gatcctgcac ctgctcttcc tgcaggcgca
      481 gatgcataaa aaaatgtctg atttttcgct gatccgtgac accatcgtca tcgtttcccg
      541 tatgatggga tatgagcagg aagatatcat ggagtacatg gagagctgtt ttcaatcccc
      601 gctttgggac tggggctatc tggcattgct ggtcatataa cctttgacat acgtatcaaa
      661 atccattaca ctaaataaca gcatccaaaa aggagaatct gttatgcttt cagctattca
      721 aaaacttttt acaacagccg ttattttaac ggcagcattt cttcttagtt cttgcggtac
      781 aatagaaaac agctccatcg gggaacctgt ttccactgtg ccggaagaca ccgaaaacct
      841 cggaacctct gcaaccgcac aaattccaaa tcctatggtt gaaattaccg atgcctcggt
      901 attttccgat aaactcggta ttacgcttga tccggactgt ttaaatgaaa atacgacaat
      961 gtacatcatc ggcgaacgac tcgctgaaat tgactataca gtcacaaatg tcgaaggcga
     1021 ggcggtttcc tgtacactcc gcgcagcaaa ggccacggaa cattgggata atctctgcgg
     1081 cgtatatgac gaaatgactg aaacaacaga agaatacgca tgcaatgaaa cgtccatcac
     1141 agttatccat aaaacaccaa aaacagaacc tgtagaaata tatgaattca cctatcagaa
     1201 tatccgttat tgttttatgt acagcggcaa gttgagcaat ctgctgacca atgaattgat
     1261 caatggtgtt ttttgtgcca tcggcgccga aactgtca
//
LOCUS       DAOSYE010000764         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_96337_length_1298_cov_3.451327, whole genome shotgun sequence.
ACCESSION   DAOSYE010000764 DAOSYE010000000
VERSION     DAOSYE010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96337_length_1298_cov_3.451327"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLU43_11345"
     CDS             complement(<1..388)
                     /locus_tag="PLU43_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOO29049.1"
                     /translation="MILLIDNYDSFSYNLYQLVGSLQPDIMVVRNDAVSIQEIETLSP
                     ACMIISPGPGRPEDAGICVEAVRHFGSRIPILGVCLGHQAICVAYGATVGYAKQLMHG
                     KQSTAVLSQESSLFAGCGKEEPVARYH"
     gene            complement(385..>1298)
                     /locus_tag="PLU43_11350"
     CDS             complement(385..>1298)
                     /locus_tag="PLU43_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOO29050.1"
                     /translation="ELTLRQMADLIRNGTPKEELSGRMTSDIRALFDKEQYCRMVEKA
                     KYHIKEGDIFQIVLSNRLEADFEGSLLNTYRVLRTINPSPYMFYFSGMDMEVAGASPE
                     TLVKLENGILHTFPLAGTRPRGKTEEEDAALERELLADEKERAEHNMLVDLGRNDIGK
                     ISRFGTVEVEEYMSVERYSHVMHIGSTVRGEIAEKYDALDTIESILPAGTLSGAPKIR
                     ACQLINDLENNKRGIYGGAIGYIDFTGNLDTCIAIRIAYKKNGKVFIRSGAGIVADSE
                     PEKEYTECMNKAAAVVDALKTAKEEMI"
BASE COUNT          303 a          373 c          258 g          364 t
ORIGIN      
        1 aatgatatct ggcaaccggc tcttccttcc cgcatccggc aaacagcgaa ctctcctgcg
       61 acaacacggc agttgactgt ttcccgtgca tcagctgttt tgcatagcca acggtcgccc
      121 cgtaagcaac acagatcgcc tgatgtccaa gacatacacc aaggatcggg atcctgcttc
      181 caaaatgcct gacagcctcc acacagatac cggcgtcctc cggtctgccc ggccccggtg
      241 aaatgatcat acatgccgga gaaagggttt caatttcctg tatactgact gcatcgttgc
      301 gaacgaccat aatatccggc tgcagggagc cgacaagctg atacaggtta taggaaaagc
      361 tgtcataatt atcgatcaga agaatcatat catttcctcc tttgctgtct taagtgcatc
      421 cacaacagct gccgctttat tcatgcactc cgtatattct ttttccggct cggaatcggc
      481 tacgatcccg gctccgctcc tgatgaatac cttgccgttc ttcttgtagg cgatcctgat
      541 cgcaatacac gtgtccagat tccccgtaaa gtcaatatag ccgatggctc cgccgtaaat
      601 accgcgtttg ttgttttcca gatcattgat cagctgacat gctctgatct taggtgctcc
      661 ggaaagcgtt cccgccggaa gaatggattc gatcgtatcc aatgcatcat atttttccgc
      721 tatttcacct cttaccgtgg aaccgatgtg catcacgtgg gaataccgct caacactcat
      781 atactcttcg acttccacag taccgaacct gctgatcttc ccgatatcat tccttccaag
      841 atccacaagc atattatgtt ctgcgcgctc tttttcgtct gccagaagtt ctctttccag
      901 cgctgcatct tcttcctccg ttttcccgcg gggtctcgtt cctgcaagcg gaaacgtatg
      961 cagaataccg ttttccagtt ttacaagcgt ttccggggat gctcctgcca cctccatatc
     1021 cattccggaa aaataaaaca tatacggcga aggattgatt gttcttaaaa ccctgtatgt
     1081 attcaaaaga ctgccttcaa aatccgcctc aagccgattt gacagaacga tctgaaaaat
     1141 atccccttct tttatatgat atttggcctt ttctaccatc cggcaatact gttctttgtc
     1201 aaacagtgcc ctgatatcgg atgtcattct ccctgacagc tcttctttcg gggtgccgtt
     1261 tctgatcaga tccgccatct gccgcaaagt aagctctg
//
LOCUS       DAOSYE010000765         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_96388_length_1298_cov_2.202735, whole genome shotgun sequence.
ACCESSION   DAOSYE010000765 DAOSYE010000000
VERSION     DAOSYE010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96388_length_1298_cov_2.202735"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1298
                     /locus_tag="PLU43_11355"
     CDS             <1..>1298
                     /locus_tag="PLU43_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOO29051.1"
                     /translation="LLTIISTYGFCNDTNDSTSVTTDTTSSVLGFPLESSTVTEIYDD
                     GGTSHQYYEINMSGMLDSGTPVPIYQLQKPTSSSTGYTCVKKYIGVGGSGYQPEGTIY
                     LGNAVGYYDSSYTPAASYVVSKDGTDYYYTESLMELSNDPVSDVLGAGSKYYCLKVTV
                     NTYENNDSVSGNSTVTDVFYACTNSQPVYSIADEAFQGRANFAQLSFPQGVVKIGDYA
                     FEGCSSLESVYLGSDCESVGVEAFANCGSITDVSFADNTMFTKIGDGAFANCAFSSIA
                     LPKDLAKIGSGAFYGCFNLNDTIDGYSSTGMYRNLNGNTPLEIGDYVFANCTSLTEMK
                     MYKNITSIGAVDITSTTTGEGTFAGCSSLERVQLPENFGSSTQTTLEGGTFYGCSSLK
                     YVRFYGTSATATDNEFITESNQESGETPNVPNSFAIWGLKP"
BASE COUNT          356 a          240 c          326 g          376 t
ORIGIN      
        1 cttttaacga tcatcagtac gtatggtttt tgtaacgata caaacgactc tacatccgtt
       61 acgacggaca caacttcttc cgtattagga ttccctttgg agtccagtac tgtaacagag
      121 atatatgatg atggcggtac ttcgcatcag tattatgaga tcaacatgtc aggtatgctc
      181 gactccggta cgcctgttcc catatatcag ctgcagaaac cgacaagcag ttcgacggga
      241 tatacctgtg ttaaaaaata tatcggtgtt ggcggaagcg gttatcagcc ggaaggaacg
      301 atctatcttg gaaatgcggt cggatattat gattcctctt atacgccggc agcgtcctat
      361 gttgtatcaa aagatggaac agattattat tatacggaat cattgatgga gttatccaat
      421 gatcctgttt cagatgtgtt aggggcaggc agtaagtatt attgtctgaa agtaacggtg
      481 aatacctatg aaaataatga ttcggtaagc ggaaattcaa cagtcaccga tgtgttttat
      541 gcatgtacaa atagccagcc tgtttattcc atagcagacg aagcatttca gggacgagcg
      601 aattttgcac agcttagttt tccgcagggt gttgtcaaaa taggagatta tgcatttgaa
      661 ggctgcagta gcctggaatc cgtgtactta gggagtgact gtgagtctgt tggtgtggaa
      721 gcatttgcca attgcggcag tattacggat gtttcatttg cagacaatac gatgtttaca
      781 aagattggag acggtgcatt tgcgaactgt gcgttttctt cgattgcatt gccgaaagac
      841 cttgcgaaaa tcggttccgg agccttttat ggctgcttta atttaaatga tacgatcgac
      901 gggtattcct ctaccggtat gtatcgaaat ttgaatggaa atacgccgct tgagatcggt
      961 gattatgtat ttgcgaactg taccagtctg actgaaatga aaatgtataa aaatataact
     1021 tcaatcggtg cggtagatat tacgtctact actaccggtg aggggacttt tgccggctgt
     1081 tcgagcctgg agagagtgca gttgccggaa aatttcggtt cttcaacgca gacgactctt
     1141 gaaggcggaa ctttttacgg ctgtagcagt ttgaaatatg tacggtttta tggtacgagt
     1201 gcgactgcga cggacaatga atttattacc gaatcaaatc aggagagcgg agaaacaccg
     1261 aatgtgccga attcgttcgc gatctggggg ctgaaacc
//
LOCUS       DAOSYE010000766         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_96469_length_1297_cov_3.047504, whole genome shotgun sequence.
ACCESSION   DAOSYE010000766 DAOSYE010000000
VERSION     DAOSYE010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96469_length_1297_cov_3.047504"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="leuD"
                     /locus_tag="PLU43_11360"
     CDS             complement(<1..353)
                     /gene="leuD"
                     /locus_tag="PLU43_11360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582675.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOO29052.1"
                     /translation="MNAKGTAFKYGDNVDTDVIIPARYLAIQDQEELAGHCMEDIDQD
                     FIKNVKKGDLIIAGKNFGCGSSREHAPIVIKVAGVSCVIAETFARIFYRNAINIGLPI
                     IECKEAAEGIEAGDEV"
     gene            complement(401..>1297)
                     /locus_tag="PLU43_11365"
     CDS             complement(401..>1297)
                     /locus_tag="PLU43_11365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOO29053.1"
                     /translation="IGADSHTCTYGALGAFSTGVGSTDMAAGMATGKAWFKVPGAIKF
                     NLVGKPAKWIGGKDVILHVIGMIGVDGALYKSMEFVGEGIRYLTMDDRFTIANMAIEA
                     GGKNGIFPVDDLTEAYMKEHSSRSYQIYEADPDAVYDEEYTIDLSKLKSTVAFPHLPE
                     NTKTVDEIEEPVKIDQSVIGSCTNGRLDDLRIAAEILKGKKIAKNVRCIVIPATQNIY
                     LQAMEEGLLKIFIEAGAVVSTPTCGPCLGGYMGILAAGERCISTTNRNFVGRMGHVDS
                     EVYLASPAVAAASAIAGYITTP"
BASE COUNT          315 a          348 c          249 g          385 t
ORIGIN      
        1 acctcatcac ctgcttcaat gccttccgct gcctctttgc attcgatgat cggcagaccg
       61 atgttgatgg cgttcctgta aaaaatacgg gcaaatgttt ctgctattac acagctgaca
      121 ccggcaacct taatgacgat cggcgcatgt tctctggaag atccgcagcc gaaattttta
      181 cccgctatga tcagatcgcc ttttttgaca tttttaataa aatcctggtc gatatcttcc
      241 atacaatgcc cggccagttc ctcctgatcc tgaattgcca gatatctggc aggaatgatc
      301 acatcagtgt caacattgtc tccatattta aaagctgttc cttttgcgtt cataattttt
      361 ctccttttct ttttcataga tcgttttttg tctgcagtac ttaaggtgtg gtaatataac
      421 cggcgatcgc acttgccgca gcaacggcag gacttgccag atatacttca gaatccacgt
      481 gtcccattct tcctacaaag ttccggttcg ttgttgagat acagcgctct ccggctgcca
      541 gtatgcccat ataaccgcca agacacggac cgcacgtcgg tgtggaaaca actgcaccgg
      601 cttcaatgaa aattttcagc agaccttctt ccattgcctg cagataaata ttctgtgtgg
      661 ctggaattac gatgcagcgt acatttttcg cgatcttttt tcctttcaga atttcagcgg
      721 caattctcaa atcatccagt cttccgttcg tacacgaacc aattacggac tgatcgattt
      781 taacaggttc ttctatttca tcaactgttt tcgtattctc tggaagatgc ggaaaagcga
      841 ccgttgattt cagctttgac agatcgatcg tatattcctc gtcataaacc gcatccggat
      901 ccgcttcata tatttggtaa gaccggctgc tgtgttcctt catatacgct tctgtcaggt
      961 catcgaccgg gaaaatacca tttttaccac cggcttcaat agccatgttg gcaattgtaa
     1021 aacgatcatc cattgtcaga taccgaatgc cttcaccgac aaattccatt gatttatata
     1081 aagcgccgtc cacgccgatc ataccgatca catgcagaat aacatctttg ccgccgatcc
     1141 attttgccgg tttcccaaca agattgaatt tgattgcacc cggcaccttg aaccatgcct
     1201 tgccggttgc cattccagcc gccatatccg tactgccgac cccggtcgaa aacgcgccaa
     1261 gtgcaccata tgtacatgta tgggaatccg ccccgat
//
LOCUS       DAOSYE010000767         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97124_length_1292_cov_2.672595, whole genome shotgun sequence.
ACCESSION   DAOSYE010000767 DAOSYE010000000
VERSION     DAOSYE010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97124_length_1292_cov_2.672595"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1292
                     /locus_tag="PLU43_11370"
     CDS             <1..>1292
                     /locus_tag="PLU43_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HOO29054.1"
                     /translation="TLAVTRNAVLSEPTVNTYSGMYDGSAHTFSVESDTEGVQITYTT
                     GISEPVSTPPSFTEPGSYTISYTLTKEGYDDYTGSGTLEIQPSADATLSALHLSVNTA
                     DYAYTPEFASDTDVYAATVDYAVTEIELLPVTTDTQASYTINGTAVGDLSSATTVSLA
                     EGKNEIPIEVTAQSGVDKKTYTLYVLRKEEAGGAAAPSNYYIAGGVFKGTAPYTISLM
                     DGSTTVAETTSSSGEFVFEGLASGIYNLSIKDSSSPVKSMIVKQTITDQNITDAKYIF
                     PTGDVNSSLEVSANTPKVRVGNLMEEALEHEVSGSQVDLTLNVSAEAAEEGTGQTAIE
                     DQADGRTLLYLKITVDLTTVDLNTGETTETPLTSTNKVLEIIIPYDFSGKENIAVYRY
                     HGSEVQEFTQLSALPESPVDLTCYLDTSNNYIYLYTSN"
BASE COUNT          373 a          279 c          338 g          302 t
ORIGIN      
        1 atacgctggc tgtgacacgg aatgctgtat tatcggaacc aaccgtaaat acatacagcg
       61 ggatgtatga cggaagtgcg catacattct cggtggagtc ggatacggaa ggtgtccaga
      121 tcacctatac gacaggaata tctgaacctg tgtccacacc gccgtccttt acggagccgg
      181 ggagctatac aatttcatat acgcttacaa aagaagggta tgatgattat acgggttccg
      241 gcacgctgga gattcagccg tcagcggatg cgaccttatc cgcgctgcat ttgtctgtga
      301 atacggcaga ctatgcgtat acgccggagt ttgccagcga tacggatgtc tatgcagcca
      361 cggtcgatta tgcggtaaca gagatagaac tgcttccggt aacaacagat acacaggcga
      421 gttatacgat aaacggaaca gcggtcggtg atctgtccag tgcgacaacg gtttccttag
      481 cggaaggaaa gaacgagatc cccatagaag taaccgcaca gtccggagta gataaaaaga
      541 catatacgct gtatgttctg cgaaaagaag aggcaggcgg agccgctgct ccttccaact
      601 actatattgc aggaggcgtt tttaaaggga ctgcgcccta tacgatcagc ttaatggatg
      661 gttcgacgac tgttgcggag acaacatcga gcagcgggga gtttgttttt gagggacttg
      721 cttccggcat atacaatctg tcgatcaagg acagttcgtc tccggtaaaa agtatgatcg
      781 taaagcagac gattacagat cagaatataa cagatgcaaa atatatcttc ccgaccggag
      841 atgtcaattc atctttggag gtttcagcga acaccccgaa agtcagggtg gggaatctta
      901 tggaggaggc gctggagcat gaagtgtccg gttcgcaggt agatcttaca ttgaacgtat
      961 cggcagaagc tgccgaagaa gggactggac aaactgcaat tgaggaccag gcagacggcc
     1021 gtacactgtt atatttgaaa atcacagttg atcttaccac agttgatctt aacacaggag
     1081 aaacgacgga aacgccgctg acgagtacaa ataaagtact ggagatcatc attccgtatg
     1141 atttttcagg caaggaaaat atcgccgtat accgttacca tggaagtgag gtacaggaat
     1201 tcacacagtt atcggccctg ccggagagtc ctgtggatct tacctgttat cttgatacgt
     1261 cgaataacta catttattta tatacgtcca at
//
LOCUS       DAOSYE010000768         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97461_length_1289_cov_3.019449, whole genome shotgun sequence.
ACCESSION   DAOSYE010000768 DAOSYE010000000
VERSION     DAOSYE010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97461_length_1289_cov_3.019449"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1289)
                     /locus_tag="PLU43_11375"
     CDS             complement(<1..>1289)
                     /locus_tag="PLU43_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOO29055.1"
                     /translation="QVMNDTAGKSVNTSLSDKGTERFDTSAKKAESNDSNSQSTETVK
                     EQDTAVGGQEQESETQETGKTEDVQNGEEITEKVSDAANEIKDAIKDKFGVTDEEIEA
                     AMETLGLSIQDLLNPDAIKELVLDLSGTEDSLSLLTNADLYDSMKEIMQMAADAATEI
                     KSEFSLTDGQFMQLLNDGDLTEGIDLKEMLSEDTGDVLQPAEEDLMQPRAETEATMDT
                     ADTADTTETAAAPVVKVYAERTEETRTSDPTGETITDSGRNEKELTVSEASQENAADN
                     QSDEQSSELFGQMTQTTTTTNNVGDVVETVKQFAQSYVNGEEVMQQVTDYIKVNISPD
                     TTSMEMQLHPESLGTVNMQITSQNGVVTAQLLVQNEAVKAVLETQLIQLQETFEQQGQ
                     KVEAVEVAVADYNLDKGLNDNSEEQSEKQKSIKLLENS"
BASE COUNT          232 a          357 c          270 g          430 t
ORIGIN      
        1 gagttttcaa gaagcttgat tgatttctgt ttttctgact gttcttcact gttatcgttc
       61 aggcccttgt ccagattata atccgctacc gccacttcta ctgcttctac cttctgtccc
      121 tgctgctcga acgtttcctg cagctggatc agctgtgttt caagtaccgc tttgactgct
      181 tcattctgca caaggagctg tgccgtcaca acaccgttct gcgatgtgat ctgcatgtta
      241 acagtgccaa ggctctccgg atgcagctgc atttccatgg aggtcgtgtc aggactgatg
      301 tttaccttga tataatcagt gacctgctgc ataacttctt cgccgtttac gtaagactgc
      361 gcaaattgct ttacagtctc tacgacatcc ccgacattat ttgtcgttgt cgtcgtctga
      421 gtcatctgcc caaacagttc gctgctttgt tcatcggact ggttgtccgc agcgttttcc
      481 tgcgaagcct ctgaaacggt cagttctttc tcgttccgcc cggaatccgt aatagtttca
      541 cccgtaggat ccgacgttct tgtttcttcg gtacgttccg catagacttt tacgaccggt
      601 gctgctgctg tttctgtcgt atctgctgtg tccgccgtat ccattgtcgc ttctgtttcc
      661 gctcttggct gcatcagatc ttcctctgcc ggctgcagga catctcccgt atcttccgaa
      721 agcatttctt tcagatcgat tccttcagtc agatcgccat cattaagaag ctgcataaac
      781 tgcccgtcag tcagggaaaa ttccgacttg atttcggtcg ccgcatctgc cgccatctgc
      841 atgatttcct tcatgctgtc gtacagatcc gcattggtca gcaaggaaag agaatcctct
      901 gtgcctgaca aatcaagcac cagctcttta atggcatcag gatttaacaa gtcctgtatg
      961 gagagcccga gcgtttccat cgctgcttcg atctcctcat ccgtcacacc aaatttgtct
     1021 ttgatggcgt ctttgatctc attcgccgca tcagatactt tttcagtgat ttcttcgccg
     1081 ttctgtacat cctcggtttt cccggtttcc tgtgtctcac tttcctgttc ctgtccgcct
     1141 acagcagtgt cctgttcctt tacggtttcc gtgctctgac tgtttgaatc gttcgattct
     1201 gctttttttg cagaagtatc gaaacgctcg gttcctttgt cagaaagcga tgtgtttacg
     1261 gactttcctg ccgtatcatt cattacctg
//
LOCUS       DAOSYE010000769         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97468_length_1289_cov_2.823339, whole genome shotgun sequence.
ACCESSION   DAOSYE010000769 DAOSYE010000000
VERSION     DAOSYE010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97468_length_1289_cov_2.823339"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..420)
                     /gene="rpsO"
                     /locus_tag="PLU43_11380"
     CDS             complement(154..420)
                     /gene="rpsO"
                     /locus_tag="PLU43_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO29056.1"
                     /translation="MIDVKKKAEIIKEYQLKEGDTGSPEVQIAVLTYRINDLNEHLKV
                     HKKDHHSRRGLLKMVGQRRNLLNYLKEKDVNRYRDLIARLGLRK"
     gene            complement(558..1019)
                     /locus_tag="PLU43_11385"
     CDS             complement(558..1019)
                     /locus_tag="PLU43_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29057.1"
                     /translation="MNNWKYRLMRFMQGRYGGDQFNFFLIIASFLLEVTGNIFGIRII
                     YWIGVAMILFAIYRMFSRNIYKRQQENIRFMSFFGRITGRKKCGGYYENRGGQNMGGN
                     RQQRENRRPKDKTVYCYYNCPSCMQQVRVPAGKGKVRIKCPKCGNVFETHS"
     gene            complement(1016..>1289)
                     /locus_tag="PLU43_11390"
     CDS             complement(1016..>1289)
                     /locus_tag="PLU43_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29058.1"
                     /translation="KAYLDDYAQIAGPKMEILLDGKGKLILGKAEDREATEMIVQKNC
                     KAYFAAFLSPKGKPISTIDSNFLRQKYLRQYSKLSKRDIGTGRHKK"
BASE COUNT          296 a          304 c          226 g          463 t
ORIGIN      
        1 gctaattctg cgctttccgc gcattacctt ctgcgcgtga tctttttgcc ttatgataca
       61 aatgacatac tgatcatacg cttcaaaatg cttttgtgct ttattgcact tataaataaa
      121 agcggagctt tactccgctt aaatttcttg aaattacttt ctcaatccca gacgggctat
      181 aagatctctg tacctgttca cgtccttttc cttcaggtaa ttgagaagat tccttctctg
      241 tccgaccatt ttaagaaggc cgcgtcttga atggtggtcc tttttgtgga ccttcaggtg
      301 ttcattcaga tcatttattc tgtaggtcag aacggcgatc tggacttccg gggatcctgt
      361 atccccttct tttagctgat actctttgat tatttcagct ttctttttta catcgatcat
      421 tttgttttct ccttttcatc atacgccttc tgcgaagttt ccgtcggagt ttcgtaaaac
      481 tcagcaaaag gtaaaaatat taacggttta tactaacaca aacatcatgt taatgcaaga
      541 gccaaactcc gggatcatca tgaatgtgtt tcgaatacat tgccgcattt ggggcatttt
      601 atacggactt ttcctttccc tgccggcacc ctgacctgct gcatgcatga ggggcagttg
      661 taatagcagt acactgtctt gtcctttggt cttctgtttt cacgctgctg tcggtttcct
      721 cccatattct gacctccacg gttctcgtaa tatccgccgc acttctttct gcctgtgatt
      781 ctcccaaaaa atgacatgaa tctgatattt tcctgctgtc ttttgtatat atttcttgaa
      841 aacattctgt aaatagcaaa gaggatcatt gcaactccta tccagtatat tatccttatt
      901 ccaaatatgt tgcctgtcac ttccagtaaa aaagacgcta ttataaggaa aaaattgaac
      961 tggtcgccgc cgtatcttcc ctgcataaat ctcatcagtc tgtatttcca gttattcatt
     1021 ttttatgcct ccctgtgcca atatctctct tggacagttt agaatactgc cttaaatatt
     1081 tttgtctgag aaaattgctg tctatggtac ttattggttt cccctttggg gaaaggaatg
     1141 ccgcaaagta tgccttacag ttcttttgaa ctatcatttc agtagcttct ctgtcttccg
     1201 ctttgcccag tatcagtttc cccttgccgt ccagcagtat ttccattttg gggccggcta
     1261 tctgggcata atcatctaaa tatgccttc
//
LOCUS       DAOSYE010000770         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97581_length_1288_cov_3.476886, whole genome shotgun sequence.
ACCESSION   DAOSYE010000770 DAOSYE010000000
VERSION     DAOSYE010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97581_length_1288_cov_3.476886"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLU43_11395"
     CDS             complement(<1..770)
                     /locus_tag="PLU43_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO29059.1"
                     /translation="MTKDLTIGKPQTILWRFTMPMFLSVIFQQLYNIADSIIAGRFAG
                     EDALAAVGASYPITMIFMAIAIGFNIGCSVIISQLFGGRQYAKMKTAVTTSLITSVIL
                     SVLLTILGLICSKSLMILISTPKNIFADGELYLRIYIGGFLFLFLYNVSTGIFTSLGD
                     SKTPLYFLIGSSVGNVLLDYLFVAIFHFGVAGVAWATFIAQGISGILALLTILTRLRT
                     IEIKGHSELFSFPMLGRIAGIAIPSILQQSFVSVGNIF"
     gene            complement(767..>1288)
                     /locus_tag="PLU43_11400"
     CDS             complement(767..>1288)
                     /locus_tag="PLU43_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO29060.1"
                     /translation="KVIMDTLHEFVKAGKVKMIGASNWRLARILEANAYAAANKKTPF
                     TVSEIQWSYVYCTNKTFHDDTLVCMEDHKEEYNAYLKAGIPVFAYSSQGHGVFSCGYQ
                     PDLSDIAAKHRDFYCEENIKRYQLLLDKCRQEQVTPSHVVLNYIMENELNGFAIVGCS
                     RLSQLKESLEAAK"
BASE COUNT          368 a          290 c          305 g          325 t
ORIGIN      
        1 ataaatatat ttccgacaga gacaaagctc tgctgcagga tacttggaat tgcgataccg
       61 gcgatcctgc cgagcatggg aaaagaaaac agttcgctat gtcccttgat ttcgatcgtt
      121 cgaagtcttg tcaggatcgt aaggagtgcg aggataccgg agatgccctg tgcaatgaag
      181 gttgcccatg caacaccggc gacaccgaaa tgaaagatcg ctacaaacag gtaatcgagc
      241 agtacgttgc cgacggaaga gccgatcaga aaatagagcg gcgttttgga atcccccaga
      301 gaagtaaaga taccggtcga aacattatat aaaaacagaa acaaaaaacc gccgatatag
      361 atccgcagat ataattcccc atccgcaaat atattttttg gagtgctgat caggatcatc
      421 agacttttgg aacagattaa accaaggatc gtcagcagta cagaaagaat aacagatgta
      481 atcaatgagg tagtaacagc tgttttcatt tttgcatact gcctgccgcc gaaaagctgt
      541 gaaatgatca cggagcagcc gatattaaag ccgatggcga tcgccatgaa gatcattgtg
      601 attggataag aggcaccgac tgcggccaaa gcatcctcgc cggcaaatct gccggcaatg
      661 atgctgtccg caatattata gagctgctgg aagatcacgc ttaaaaacat cggcatcgta
      721 aaccgccaga gaatcgtctg cggtttaccg attgtcagat cctttgtcat tttgccgcct
      781 ccagagattc cttcaactgg gaaaggcggg agcagccgac aatggcaaag ccgttcagtt
      841 cattttccat aatataattt aagaccacat gggaaggggt cacctgttcc tgtctgcatt
      901 tatctaataa cagctgatat cttttgatgt tctcctcgca gtagaaatcc cggtgtttgg
      961 cggcgatatc ggagagatcc ggctgatagc cgcaggagaa aacaccatgt ccctgtgagg
     1021 aataagcaaa caccggaata ccggctttca gataggcgtt gtattcctcc ttatgatcct
     1081 ccatgcagac aagtgtgtcg tcatgaaagg ttttgtttgt gcagtagaca tagctccact
     1141 ggatttcaga tactgtgaac ggtgtctttt tatttgccgc tgcataggcg tttgcttcca
     1201 gtattcttgc cagtctccag ttggaagcgc cgatcatttt gacttttccg gctttaacaa
     1261 actcatgcag tgtatccatg atcacttt
//
LOCUS       DAOSYE010000771         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97698_length_1288_cov_1.763179, whole genome shotgun sequence.
ACCESSION   DAOSYE010000771 DAOSYE010000000
VERSION     DAOSYE010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97698_length_1288_cov_1.763179"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>1288)
                     /locus_tag="PLU43_11405"
     CDS             complement(181..>1288)
                     /locus_tag="PLU43_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO29061.1"
                     /translation="KVVKRLTAGVEALLKSNGVEMYSGEAFIHPDKTVSAGNVKVSAD
                     KVIFAGGSKAVKINIPGIELEHVLTSDEILDLDHVPKRLVIIGGGVIGVELASVFAAF
                     GSKVAIIELMDNVLPMMDNDISSAICKSLNKKGIKTYTGTKLEKIEEADGYLKVYSDK
                     QEPIDTEYVLLSIGRIPDLTAIQELDLDKDSGRIKVDDTMRSSLDWLYAPGDINGRSM
                     LAHSAFKMGETAAENAMGHSKKVNLSYVPSCVYTSPEVGSVGMTEQQAQTNYDVSIGM
                     FPFQANGRALASGEIEGYVKVITDKKYGEVLGVHIVGPSATELINEAAALMSMEVTAN
                     EIADIIHAHPTYAEAFMEAAADSISECLHLPKNK"
BASE COUNT          321 a          316 c          230 g          421 t
ORIGIN      
        1 gtgtgttgta tgggctcatt cttatttcca ttcactttac cttaactttc tgtactgaaa
       61 tctatatgat cgtatttttt gcatactgtt atttaatcta aataaactta aatcctctta
      121 ctctcttata gaataacatg atttcatcat tatttgcgct gtttaataaa actctttcaa
      181 tcatttattt ttaggcaggt gcaagcattc tgaaatacta tctgccgcag cctccataaa
      241 agcttctgca taagtcgggt gcgcatgaat gatatctgca atttcatttg cagtgacctc
      301 catactcatc agagcagcgg cttcgtttat cagttctgtg gcagatggcc cgacaatatg
      361 cacaccaagc acttcgccgt actttttgtc ggttattacc ttaacataac cttcgatttc
      421 accagatgca agcgcacgtc cattagcctg aaaagggaac ataccgatac ttacgtcata
      481 gttcgtttgt gcctgctgtt cggtcatccc aacgcttccg acttcaggcg atgtatacac
      541 gcaagacggt acataggaaa ggttaacttt tttactgtgt cccatcgcat tctcagctgc
      601 cgtttctccc attttgaacg cagaatgcgc aagcatgctt cttccgttaa tatctcctgg
      661 cgcatatagc caatccaaac tggatctcat cgtatcatct accttaatgc gtccggaatc
      721 tttatctaaa tcaagttcct gaattgcagt aagatcaggg atgcgcccga tagacagcaa
      781 tacgtattcc gtatctatag gttcctgttt gtccgaatat actttcaaat agccatctgc
      841 ttcctctatt ttttcaagct ttgtgcctgt ataggttttg ataccttttt tattcagtga
      901 cttacagatg gcgctggata tgtcgttatc catcatagga agcacattat ccatcaattc
      961 tataattgcg actttgctgc cgaatgccgc aaacaccgat gccaattcca cgccgatcac
     1021 accgcctccg ataatcacaa ggcgtttagg tacatgatcg agatcaagta tttcgtcgct
     1081 ggtcaatacg tgctctaatt caataccggg gatgtttatc tttacggctt tcgatccgcc
     1141 tgcaaagata actttgtcgg ctgatacctt tacattgcct gcagaaactg tcttgtcagg
     1201 atggatgaac gcttctccgc tatacatttc tactccgttg ctcttcagca atgcctcaac
     1261 gccggccgtt aaccgcttta caactttg
//
LOCUS       DAOSYE010000772         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97742_length_1287_cov_3.468344, whole genome shotgun sequence.
ACCESSION   DAOSYE010000772 DAOSYE010000000
VERSION     DAOSYE010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97742_length_1287_cov_3.468344"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLU43_11410"
     CDS             <1..876
                     /locus_tag="PLU43_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOO29062.1"
                     /translation="PIEVLNEAFQENTRCVIAETIANPALVVLDIEKFADAAHAHGVP
                     LIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAMCVGGCIVDSGNFDWSAYADKYP
                     GLTEPDDSYHGVVYTERFGKGAYITKATAQLMRDLGSIQSPQNAFLLNVGLETLHLRM
                     PRHCENALKVAQYLKNHPAIAWVAYPGLEDDKYHALAEKYLPNGTCGVLCFGLKGGRN
                     ASVGFMDRLKLAAIVTHVADARTSVLHPASHTHRQMTDEQLVEAGVAPDLIRFSVGIE
                     NVEDIIADLEQALER"
     gene            883..>1287
                     /locus_tag="PLU43_11415"
     CDS             883..>1287
                     /locus_tag="PLU43_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29063.1"
                     /translation="MREKGDVFSIITAVLTLAVLGYVLFISVFNQIVAQNEGFFYMIV
                     FGGILVLAFLLLSLMTRLFAISNEFEDRKLWHVLEAVVLVLLAIFFIRSRLSYTSSLP
                     AEETVFYRTAVLMNKQTLSVAGMDLIPQLMRNP"
BASE COUNT          326 a          245 c          357 g          359 t
ORIGIN      
        1 ccgattgaag tattgaatga agcatttcag gaaaatacaa gatgtgtgat agcggaaacg
       61 attgcaaatc cggcgcttgt tgtactggat attgaaaaat ttgccgatgc ggcacatgcg
      121 cacggcgtgc cgttgatcgt ggataatacg tttgccacac cgatcaactg ccggccattc
      181 gaatggggtg ctgatatcgt aacacattcc acaacaaaat atatggatgg tcacgcgatg
      241 tgtgttggcg gctgtattgt ggacagcggt aattttgact ggagtgccta tgcggataaa
      301 tatcccggac ttacagagcc ggatgattcc taccatggtg ttgtttatac ggagcggttc
      361 ggaaagggtg cgtatattac aaaagcaact gctcagctga tgcgtgatct tggctcgatc
      421 cagtctccgc agaatgcgtt tctgctgaat gtaggtctgg aaacactgca tctgcgcatg
      481 ccgagacatt gtgaaaatgc attaaaagta gcgcagtatc tgaaaaatca tcctgcgatc
      541 gcctgggtgg cgtatccggg actggaagac gataaatatc atgcccttgc ggaaaaatat
      601 ctgccgaatg gtacatgcgg agtactttgt tttgggttaa agggcggcag gaatgcttcc
      661 gtgggcttca tggacagact gaagctggct gcaattgtta cacatgtggc ggatgcaagg
      721 acaagcgtac tgcatccggc cagtcataca caccggcaaa tgacggacga gcagttggtt
      781 gaagccggtg ttgcacctga tttgatcaga ttttccgttg gtattgaaaa tgttgaagat
      841 attattgcgg atttggagca ggccttggaa agatagggga ctatgagaga aaaaggtgat
      901 gttttttcaa taataacggc agttcttaca ctggctgtgc ttggttatgt attattcatt
      961 tccgtgttca atcagattgt cgcgcagaac gaagggttct tctacatgat cgtgtttgga
     1021 ggaatccttg ttttagcgtt tttattattg agtctgatga caaggctctt tgcgatatcc
     1081 aatgaatttg aagacagaaa gctgtggcat gtgcttgagg cggtcgttct tgtcctgctg
     1141 gcgatttttt ttatcaggag ccggttgtct tatacgtcgt cactgccggc tgaggaaact
     1201 gtattttaca ggacagccgt attgatgaat aagcagacac tgagtgttgc agggatggat
     1261 ctgataccgc agctgatgcg gaacccg
//
LOCUS       DAOSYE010000773         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_97801_length_1287_cov_2.322240, whole genome shotgun sequence.
ACCESSION   DAOSYE010000773 DAOSYE010000000
VERSION     DAOSYE010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97801_length_1287_cov_2.322240"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1287)
                     /locus_tag="PLU43_11420"
     CDS             complement(<1..>1287)
                     /locus_tag="PLU43_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29064.1"
                     /translation="DIGMAVVFEFSNYIMEEQPLVKVSEKTDYQILIYLGNENEENIW
                     KSLMQDFKISPIYVSGPSAFRTAIENKRYTQIFIPDSVYDLLKDILISAQCEDYTYVV
                     TNYRHVYHDYGKSRIIRRPLFCLNFSEAINGTWAKEDYQKSMGGEQVVFPNAKVLVVD
                     DSMVNLRVMLVMLDNYKIKADMAVSGENCLKLLETERYDLILLDRRMPEMDGVETLHK
                     IRQMDGNEHIPVLCITADFGAEIRERLMAEGFQDYLAKPVKGYYLERLLRQYLPEHLS
                     LCISEEEAKDLPQKEESRKSAAPAEDPRVLDTVKGTENIGGNEEAYHVILNTYYREGI
                     RKLEEIPLLDSNDDLSLYITNVHSLKSSSASVGALGISALFKELEIAGKENDRAFLEE
                     HTKDVLSRFSVLLENVKAYLTENGVFESDMYEEERPG"
BASE COUNT          333 a          346 c          212 g          396 t
ORIGIN      
        1 tcccggcctt tcttcttcat acatatcact ttcaaaaact ccattttcag tcagatacgc
       61 tttcacattt tccaaaagga cagaaaaccg ggacaaaacg tcttttgtat gttcctctaa
      121 aaacgctctg tcattttcct ttccggcaat ttcaagttct ttaaacaaag ccgaaattcc
      181 aagcgccccg acactcgcac tgctgctctt taagctgtgg acatttgtaa tatacagtga
      241 caggtcatca ttgctgtcca gcagcggaat ctcttccagc ttacggatac cttcccgata
      301 atacgtattc aggatcacat gataggcctc ttcatttcct ccgatatttt cggttccttt
      361 cactgtatcc agcacacgcg gatcctctgc cggcgccgca cttttccggc tttcttcctt
      421 ctgcggtaaa tcctttgctt cttcttcgct gatacacaat gacaaatgtt caggcaaata
      481 ctgccttaac agccgttcca gataatagcc ttttaccggt ttcgcaagat agtcctgaaa
      541 gccttccgcc atcagccgtt ccctgatctc ggccccaaaa tccgcagtta tgcatagtac
      601 cggtatatgc tcgttgccgt ccatctgcct gatcttatga agtgtttcca ccccgtccat
      661 ttccggcata cgccgatcaa gcagaatcag gtcatagcgt tctgtctcca gcagcttcag
      721 acagttttcg ccgcttacag ccatatctgc ttttatttta taattgtcaa gcatcacaag
      781 cattactctt aaattgacca tactatcgtc aaccacaagc actttggcat taggaaaaac
      841 aacctgttct cctcccatgg atttctggta atcttccttt gcccatgtac cgttaattgc
      901 ttctgaaaaa ttcagacaga acagcggtct tctgataatt ctgctttttc cgtaatcgtg
      961 atatacgtgc ctgtaattcg tcacaacata ggtgtagtcc tcacattgtg ccgaaatcag
     1021 tatatccttt aaaagatcat atacggaatc cggaatgaat atctgtgtgt atcgtttatt
     1081 ttcgatcgct gtccgaaaag cactcggacc ggacacataa atcggactga tcttaaaatc
     1141 ctgcataagg gatttccaga tattctcctc attctcatta cccagataaa tcaatatctg
     1201 atagtcggtc ttctccgata cttttacaag cggctgttcc tccataatat aattggaaaa
     1261 ttcaaaaaca accgccatac caatatc
//
LOCUS       DAOSYE010000774         1284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_98119_length_1284_cov_3.157038, whole genome shotgun sequence.
ACCESSION   DAOSYE010000774 DAOSYE010000000
VERSION     DAOSYE010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98119_length_1284_cov_3.157038"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLU43_11425"
     CDS             <1..415
                     /locus_tag="PLU43_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29065.1"
                     /translation="ALINEAQAHTDHMINEHEIMQQAYAQANEVVQQAVNQAQQILDS
                     ATEDANGIRQSSMKYTDDMLAELQNIVTHAVETAEAKYGSLITQLKECEAIISKNRSE
                     LYPQVELDSEIASLEGKPSGENVAGDNISLDMLNH"
     gene            503..>1284
                     /locus_tag="PLU43_11430"
     CDS             503..>1284
                     /locus_tag="PLU43_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO29066.1"
                     /translation="MNVLENLKPEKVFQYFEKICQIPHGSGNTESISRYCEDFAKARG
                     LAYNRDRSGNIVIYKSGSCGYEDSAPVILQGHLDMVCENDFEYESRHDFKKDPLHLAI
                     MDDYVYAKGTTLGGDDGIAVAYMLAILDDNSLSHPPLEAVFTVDEEIGMKGAAIFDTS
                     CLCGKKMLNIDNEMEGELLTSSAGGSMVICEIPVRYGTKQGIKYNLLICGLQGGHSGT
                     EIDKYRGNANLLMGRLLHYIGTKINYDLSYLKGGLQDNAIPR"
BASE COUNT          387 a          259 c          326 g          312 t
ORIGIN      
        1 agctctgatc aatgaagcgc aggcccatac ggatcatatg atcaatgagc atgagatcat
       61 gcagcaggct tatgcacagg caaacgaagt cgttcagcag gcagtcaatc aggcacagca
      121 gattttggac agtgcgactg aggacgccaa cggcatccgc cagtcttcca tgaaatatac
      181 tgacgacatg cttgcggaat tgcaaaacat cgtcacacac gcagtcgaaa ctgccgaagc
      241 aaaatatggc tcgctgatca cacagctgaa agaatgcgaa gcaattatca gtaaaaatcg
      301 ttccgagctg tatccgcagg tggaactgga cagtgagatc gcatcattgg aagggaaacc
      361 ttccggtgaa aatgtggcag gtgataatat cagcctggat atgctgaatc attaagagaa
      421 tactgtttct gtctggagct gtttcggaac cgatatatat ttcggttccg tatttataat
      481 ctattgaaaa ggatgagaac gcatgaatgt tttggaaaat ttaaaaccag aaaaggtatt
      541 tcaatatttt gagaagatct gtcagatccc gcatggctcc ggcaatacgg aatcaatcag
      601 caggtactgt gaagattttg caaaagcacg cggccttgct tataacaggg atcgttccgg
      661 aaatatcgtg atctataaat ccggcagctg cggatatgaa gattcagctc ctgttatttt
      721 acagggacat ctggatatgg tctgcgaaaa tgattttgaa tatgaaagca ggcatgattt
      781 caaaaaagat ccgcttcatc ttgcgatcat ggatgattac gtctatgcaa aaggcactac
      841 gctcggcggg gatgacggca tagcagttgc ctatatgctg gcgatactgg atgacaacag
      901 tttgtcacac cctccgctgg aagccgtctt cacggtcgat gaggagatcg gaatgaaggg
      961 cgcagccatt ttcgatacat cctgtctgtg cggtaaaaaa atgctgaata tagataatga
     1021 aatggaaggg gagctgttga cgagcagtgc cggcggcagt atggtcatct gtgagatccc
     1081 ggtcagatac ggcacaaagc aggggattaa atataatctg ctgatctgcg gcctgcaggg
     1141 cggacattcc ggaactgaga tcgataaata ccgcggaaac gccaatcttc tgatgggaag
     1201 gcttcttcat tatattggaa caaaaatcaa ttatgatctg tcttatttaa aaggcggtct
     1261 gcaggataat gccattccaa gaga
//
LOCUS       DAOSYE010000775         1282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_98354_length_1282_cov_3.958435, whole genome shotgun sequence.
ACCESSION   DAOSYE010000775 DAOSYE010000000
VERSION     DAOSYE010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98354_length_1282_cov_3.958435"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLU43_11435"
     CDS             complement(<1..713)
                     /locus_tag="PLU43_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO29067.1"
                     /translation="MKDRILLFIPAYNCQKQIIRVLGQLDADLMHYITGVIVVNNRST
                     DDTELVVKGFMERHPDIPVTLLRNKENYGLGGSHKVAFSYAAEHTYDYVIVLHGDDQG
                     TLEDFLPVLKSRYYTKHDCILGARFMRGSRLEGYSVFRTFGNIVYNFLFAAVVRRRIF
                     DLGSGLNMYSMKMLREAYYEKFPDNLMFNYCMILAGEYYRQDIRFYPVSWREEDQVSN
                     VRMINQAMTVLKMLFDYYR"
     gene            complement(755..>1282)
                     /locus_tag="PLU43_11440"
     CDS             complement(755..>1282)
                     /locus_tag="PLU43_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29068.1"
                     /translation="QARAQVWLESNDYYIRKQYVIWLQGESDAEDGITSEKYNENMDN
                     IIRPLFIGGVQKVFFITPGRTISKNQYFNNIISAQLEMSKTSGYYALGTTVLNGVSTE
                     YMTDEWHYNQTVLNLVGSETAKSVAYYTNNQKEACVYDYKNLTTFIPDGFDYPEDTVV
                     EPLDVNDSGLLQKLQ"
BASE COUNT          356 a          333 c          231 g          362 t
ORIGIN      
        1 tccctgtaat aatcaaacaa catctttagc acagtcattg cctgatttat catccgtaca
       61 ttcgatacct ggtcttcttc cctccaggat accggataaa aacgaatatc ctgtctgtaa
      121 tattcaccgg caaggatcat acagtaatta aacatcagat tgtccggaaa tttctcataa
      181 tatgcttccc gcagcatttt catgctgtac atattaagac cggagcccag atcaaaaata
      241 cgtctgcgga caacggctgc gaacagaaaa ttatatacaa tattgccgaa ggtccggaag
      301 accgaatagc cttccagcct ggaacccctc ataaaacggg ctcccagaat acagtcatgt
      361 tttgtataat atcggctttt cagcaccggc agaaaatcct ccagcgttcc ctgatcatcc
      421 ccgtgcagaa ctatcacata gtcgtaggta tgttctgctg cataggaaaa cgcaacctta
      481 tgggaaccgc cgagtccgta attttcctta tttcgaagaa gtgttacagg aatatccgga
      541 tgtctttcca taaatccctt tacaacaagc tccgtatcat ccgtactccg gttgttcaca
      601 acgatcacac cggtaatata gtgcatcaga tccgcatcca gctggccaag taccctgatg
      661 atctgttttt ggcagttgta cgccggtata aaaagcaaaa tcctgtcctt cataactggc
      721 ctccgttcct gtgaaaccct tccataataa agtattactg aagcttttgc agcagtccgc
      781 tgtcgtttac atcaagcggt tccacgaccg tatcctccgg ataatcaaag ccgtccggaa
      841 taaaagtcgt cagatttttg taatcataga cacaggcctc tttctgattg ttcgtgtagt
      901 aagccacgct ttttgctgtc tcggaaccaa caaggttcaa aactgtctga ttataatgcc
      961 attcgtctgt catgtattcc gtactgacac cgttcaaaac agtagtcccc agcgcataat
     1021 aaccgcttgt tttactcatt tcaagctgtg cactgataat attgttaaaa tactgatttt
     1081 ttgaaatcgt ccgtcccggc gtaataaaga ataccttctg cacccctccg ataaacagcg
     1141 ggcgaataat attatccata ttttcgttat atttttcaga agtgatccca tcctcagcat
     1201 ccgattcccc ctgcagccag atcacatact gcttccggat ataataatca ttgctctcca
     1261 gccagacctg tgcacgggcc tg
//
LOCUS       DAOSYE010000776         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_98525_length_1281_cov_2.575856, whole genome shotgun sequence.
ACCESSION   DAOSYE010000776 DAOSYE010000000
VERSION     DAOSYE010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98525_length_1281_cov_2.575856"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLU43_11445"
     CDS             complement(<1..1254)
                     /locus_tag="PLU43_11445"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOO29069.1"
                     /translation="MASCSYDNWKKTETVVFDKTVLKAQSGAKVQRMTKLPAERIFQT
                     PQGDTVVDFGQNLTGWVEFTVQGSAGDEVELNFFEVLDAAGNVYLDNLRTAKETVTYI
                     IKDDNEVTFAPHFTYQGFQFMRLAKYPGEPKPENFTACALHSEMEQTGTFTCSNPDVN
                     QLMHNILWGMKGNFLDVPTDCPQRDERLGWTGDAQIFCRTATYLMDTYTFYTKWLRDV
                     AVDQTEEGGVPHVVPDILTGKSTEDWLMKDGAHSAAAWADVAVIMPWTLYLMYGDKTV
                     IENQYESMKAWLGFMQAHAEDHIWNYKLQFGDWVALDAEEGSYFGATPTDLTCTAYYA
                     YSTGLFAKMAKAVGKDADAENYQKLYEEIVKSYQKHFFDEDGRLIAQTQTAQIVSLYF
                     DLVQKESVQKVTEDLLRLLEKEHGHL"
BASE COUNT          305 a          369 c          248 g          359 t
ORIGIN      
        1 cagatgtccg tgttccttct caagcagcct aagcaggtct tctgtaacct tctgcacact
       61 ttccttctgc acaagatcaa aataaagcga aacgatctgc gcggtctgcg tctgtgcgat
      121 cagtctgcca tcctcatcaa agaaatgctt ttgatagctt tttactatct cttcatacag
      181 tttctgatag ttctctgcat ccgcatcttt cccgactgcc tttgccattt tggcaaacag
      241 accggtcgaa tacgcataat aggccgtaca cgtcagatct gtcggggttg ctccaaaata
      301 actcccttcc tccgcatcaa gcgctaccca gtcaccaaac tgcagcttat agttccatat
      361 atgatcctct gcatgtgcct gcataaatcc aagccatgcc ttcatgcttt catactggtt
      421 ctcaatcact gttttgtcgc catacatcag atacagtgtc cacggcataa ttacggcaac
      481 atccgcccat gccgcggctg aatgcgcacc gtccttcatc agccagtcct ctgtggattt
      541 tccggtcaga atatccggaa ctacatgcgg aacacctccc tcttctgtct gatccacagc
      601 tacatcccgc agccatttcg tataaaaagt ataggtatcc atcaaatacg ttgcggtcct
      661 gcagaagatc tgcgcgtccc cagtccagcc gagccgttcg tcacgctgcg ggcagtctgt
      721 cggaacatcg agaaaattgc ccttcatgcc ccataaaata ttatgcatca gctgattgac
      781 atccggattg gagcatgtaa aggtgcctgt ctgttccatt tcggaatgca gcgcacaggc
      841 agtaaagttt tcaggctttg gttctcccgg atactttgcc agccgcataa actgaaagcc
      901 ctgatatgta aaatgaggtg caaatgtaac ctcgttgtca tcttttataa tataagtcac
      961 cgtctcctta gccgtacgca gattatccag atagacattt ccggcagcat ccaatacttc
     1021 aaaaaaattc agttccactt catcgccggc actgccctgt acggtaaatt ccacccagcc
     1081 ggtcagattc tggccaaaat caacgaccgt atctccctgc ggtgtctgaa agatccgttc
     1141 tgccggtaat tttgtcatcc tctgcacttt agcaccgctc tgcgctttca gcactgtttt
     1201 gtcaaatacg acagtttctg ttttcttcca gttatcataa ctgcaggatg ccatgcacca
     1261 gtccgggatc tcctttctcg c
//
LOCUS       DAOSYE010000777         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99138_length_1276_cov_3.362817, whole genome shotgun sequence.
ACCESSION   DAOSYE010000777 DAOSYE010000000
VERSION     DAOSYE010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99138_length_1276_cov_3.362817"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..279)
                     /locus_tag="PLU43_11450"
     CDS             complement(19..279)
                     /locus_tag="PLU43_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29070.1"
                     /translation="MGTMGRTKTEKFKNLCIFALPGIFIFCMVVMVPFLYGFYLTFTD
                     WDGVKKIKNLVGFSNYAAVFQDANFWSSMELTLRYVFFLTIQ"
     gene            complement(377..>1276)
                     /locus_tag="PLU43_11455"
     CDS             complement(377..>1276)
                     /locus_tag="PLU43_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29071.1"
                     /translation="SYAFLFIASSLIYNKKAIEDTLGTEFDPAAINSYDALKALLEDL
                     RAGGMEDPVVISKEDWSLGAHQLGFIYDTYDGTTAGSAELIGKLEDGSLAVEDYERYT
                     EFMDTLDLLLEYNINKEDPLGALYEQDPIFLADGDAAIWANGCWAWPNIEEAGASADD
                     EYGFLPFVLGNDTSDFANNGIEAAATKQVMIDNVQATEEQQAAAKEFLNWIVYEDNGQ
                     RMLVEDAAVIPACKNNAYDPEDPLGMDIKNKMASGNTYSSCYVAPSDHWSVLGAAMQK
                     YIAGESSREDLAKDIDTYWTSQQ"
BASE COUNT          346 a          324 c          233 g          373 t
ORIGIN      
        1 agataatttc tgataatttt attgaatcgt taaaaaaaat acatagcgaa gtgtcagctc
       61 catagacgac cagaaattag catcctgaaa taccgccgca taatttgaaa aaccgaccag
      121 attttttatt ttctttaccc cgtcccaatc cgtaaatgtc agataaaacc catacaaaaa
      181 cggaaccatc acaaccatgc aaaaaataaa aatccccggc aaagcaaaaa tgcagagatt
      241 tttaaatttt tctgtttttg ttctacccat tgttcccata agcatatcct ttctgtgaaa
      301 ttcctgttaa cgttttactt taccgatttc ctgtcaattt cgggcaccgt atttaccggt
      361 gcccgaaacg atgctgctac tgctgtgatg tccaataggt gtcgatatct tttgccagat
      421 cttctctgga gctctctcct gcaatatact tctgcattgc tgctccgagc acgctccaat
      481 gatcggacgg tgcaacatag cacgatgaat aggtattgcc ggatgccatt ttatttttga
      541 tatccatacc gagcggatct tccggatcat aagcattgtt cttgcatgcc ggaattacgg
      601 ccgcatcttc cacaagcatt ctctgaccgt tgtcttcata cacgatccag ttgaggaatt
      661 cctttgctgc tgcctgctgc tcctcggtag cctgtacatt gtcgatcatg acctgctttg
      721 ttgccgctgc ctcaattccg ttattggcaa aatcagaagt atcatttcca agtacaaacg
      781 gaaggaatcc atactcgtcg tctgcagaag cacctgcttc ttcaatattc ggccatgccc
      841 agcatccgtt tgcccagatc gcagcgtctc cgtccgcaag gaaaatagga tcctgttcat
      901 acagggcgcc aagcggatct tctttgttga tattgtattc aagcagcaaa tcaagtgtat
      961 ccataaattc cgtataacgc tcataatctt ctaccgcaag agaaccatcc tccagctttc
     1021 cgatcagctc tgcgctgccg gccgttgttc cgtcatacgt atcataaata aatccgagct
     1081 gatgtgcgcc aagtgaccag tcttcctttg aaatcacgac cggatcttcc attccgcctg
     1141 cacgcaggtc ctctaacagt gctttgagtg cgtcataaga attgatcgca gccggatcaa
     1201 actctgttcc aagcgtatct tcaattgctt ttttattata aattaaacta gaagcgataa
     1261 aaagaaaagc ataact
//
LOCUS       DAOSYE010000778         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99160_length_1276_cov_2.850942, whole genome shotgun sequence.
ACCESSION   DAOSYE010000778 DAOSYE010000000
VERSION     DAOSYE010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99160_length_1276_cov_2.850942"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1276
                     /locus_tag="PLU43_11460"
     CDS             <1..>1276
                     /locus_tag="PLU43_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29072.1"
                     /translation="QIIVKKMRSDISKKINRLPLKYFDSVSVGDVLSRVTNDADTIGQ
                     TLNQSVGTLFISITLLAGSLIMMLYNNLILALTAAGASTAGFLLMLLIMKVSQKYFIE
                     QQKELGRINGHIEEVYAGHQVIKAYNGGREAKAVFEKSNQRLYGSAWRSQFLSGLMMP
                     LMNMAGNLGYVAVCVVGAVLAVNGKITFGVIVAFMMYIRLFTHPLSQIAQAMNSLQRT
                     AAASERVFEFLNESELQEEPEKQNRLADANKDVEFWNVRFGYAPAKPVIHSFTANIKE
                     GQKVAVVGPTGAGKTTLVNLLMRFYETDGGEIRIGGIPLHKVPREDVHAQFGMVLQDT
                     WLFEGTIKENVIYGSQDVAMEEVVKACRTVGLHHFIKTLPDGYDTVINDKTALSEGQK
                     QLITIARAMIRNAPLLILDEATSSVDTRTERLV"
BASE COUNT          340 a          262 c          373 g          301 t
ORIGIN      
        1 cgcagatcat cgtgaagaaa atgaggagtg atatctccaa aaaaataaat cgtctgccgc
       61 tgaaatattt tgattcggtc agtgtcggag atgtattgag ccgtgtgacc aatgatgcgg
      121 atacgatcgg ccagacgctg aatcagagcg ttgggacgct gttcatttcc ataacgctgc
      181 tggccggttc tttgatcatg atgctttata acaacctgat actggcattg actgccgcag
      241 gtgcaagtac tgccggattt ttgcttatgc tgctgatcat gaaagtatca cagaaatatt
      301 ttatagagca gcagaaagaa ctgggacgca tcaatgggca tattgaagag gtctatgcgg
      361 gacaccaggt gatcaaggct tataacggcg gacgggaggc gaaagcggtt tttgaaaaaa
      421 gcaatcagag gctgtacggc agtgcatgga gatcccagtt tttgtccgga ctgatgatgc
      481 cgcttatgaa tatggcgggg aatctcggtt atgtagcggt ctgtgttgtc ggtgcggtgc
      541 ttgcggtgaa cggaaagatc acgtttggcg tgatcgtggc atttatgatg tatatccgcc
      601 tgtttacaca tccgctgtct cagatcgcac aggcaatgaa cagcctgcag cggactgctg
      661 cggcaagcga acgggttttt gagtttctga acgaatcgga attgcaggaa gaaccggaga
      721 agcagaacag gcttgccgat gcgaataagg atgtggaatt ttggaatgtc agatttggtt
      781 atgcgccggc gaagccggtg atccacagct ttacggccaa tataaaagag ggccagaagg
      841 ttgctgtcgt aggaccgaca ggagccggaa aaacaacgct tgtgaatctg cttatgagat
      901 tttacgaaac agacggcggc gagatcagga tcggcgggat cccccttcat aaagtaccaa
      961 gggaagacgt tcatgcgcag ttcggcatgg ttttgcagga cacgtggctt tttgaaggaa
     1021 cgataaaaga aaatgttatt tacggcagtc aggacgttgc catggaagag gtcgtcaagg
     1081 cgtgcaggac cgtcggactc catcatttta tcaaaacact gccggacggc tatgatacgg
     1141 tcatcaatga taaaacggct ctttcggaag ggcagaagca gttgatcacc attgcacggg
     1201 caatgatcag gaatgccccg ctgctgattc tggacgaagc gaccagttcg gtcgacacgc
     1261 gtacggaacg gctcgt
//
LOCUS       DAOSYE010000779         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99224_length_1276_cov_2.073710, whole genome shotgun sequence.
ACCESSION   DAOSYE010000779 DAOSYE010000000
VERSION     DAOSYE010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99224_length_1276_cov_2.073710"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..>1276)
                     /locus_tag="PLU43_11465"
     CDS             complement(260..>1276)
                     /locus_tag="PLU43_11465"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOO29073.1"
                     /translation="KMYRPAPAFFTDEMDKEALQLYQTFCPGIIEELKGFSEESNIPM
                     REMSYEWMTYLVPGCCGICLPGSRMSDGHTRILRSYEFGIEEEDLTVCRTSIPGKYTH
                     IGTTIATFGRGEGINEHGLAVAQSSCGFPVSNLPQMRRPAIKGLQFWAVIRTLLENCK
                     SVDEALEMLKDMPIAYNINLYLADSSGMTALYETMNGAAAFEKSPGDKKDCLFGTNHI
                     VIKSFQNREPAAMRNSVLRYERIREFLQETGTKTEEECRAFLLKKYPKGPSACYYDEW
                     FGTIRSAVLDTKEKRFSICWFGREENGWEDYFADQELQVCEKEKTLVREKAERGFFEV
                     IPLI"
BASE COUNT          319 a          338 c          233 g          386 t
ORIGIN      
        1 cgacgatcag caccttctcc ccctggtaaa cggaaaggtt aatattggtg atcgtaggtt
       61 ccgcctgtgc aaaatattga aaggaataat cggcaaactc aatcaccggc cgcttctctg
      121 tcatcggata tgcctctttt ctcaaatata gcagaaggat tctgcaaata ttccttctgc
      181 taaattaacg tagcatactg acaggttcct gtcaatatcc ggtcttttca gtgtttcatt
      241 tacgatcaga aaaacaatgt catatcaatg ggattacttc aaaaaacccc cgttctgcct
      301 tttcacgcac caatgttttt tctttttcac aaacttgtaa ttcctggtcc gcaaaataat
      361 cttcccagcc gttttcctca cgaccaaacc agcagatact gaatcgtttt tcttttgtat
      421 caagcactgc cgacctgatc gttccaaacc actcatcata ataacaagcg gaaggtcctt
      481 tcggatattt tttcagtaaa aaagctctgc actcttcttc tgtttttgtt cccgtctcct
      541 gcagaaattc tctgatccgt tcatacctga gcacggaatt acgcatcgcc gcaggttctc
      601 tgttttgaaa tgatttgatt acgatatgat ttgttccaaa aagacagtct tttttatctc
      661 ccggcgattt ttcaaaagct gccgctccgt tcatcgtctc atataatgca gtcatgccgg
      721 aggaatcagc tagataaaga ttgatattat aggcgatcgg catgtctttg agcatctcaa
      781 gcgcttcatc aacacttttg cagttttcca gaagcgtcct gataactgcc cagaactgaa
      841 gaccctttat tgccggcctc ctcatctgcg gcagattgct gaccggaaac ccgcaggaag
      901 actgcgccac tgccagacca tgctcattga ttccctcacc cctgccaaat gttgcgatcg
      961 tagttccgat atgcgtatat tttcctggga tcgatgtcct gcagacggtc agatcttctt
     1021 cttcgatccc aaattcatag ctgcgcagaa ttcttgtatg cccgtccgac atcctgctgc
     1081 ccggcaggca gatcccgcag cagcccggta caagatatgt catccattca taggacatct
     1141 ctctcatcgg tatattgctt tcctccgaaa accctttcaa ttcttcaata attcccgggc
     1201 agaacgtttg atacagctgt aatgcctctt tatccatttc atccgtaaaa aatgccggag
     1261 ccggtctgta catttt
//
LOCUS       DAOSYE010000780         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99295_length_1275_cov_3.350000, whole genome shotgun sequence.
ACCESSION   DAOSYE010000780 DAOSYE010000000
VERSION     DAOSYE010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99295_length_1275_cov_3.350000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLU43_11470"
     CDS             <1..318
                     /locus_tag="PLU43_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOO29074.1"
                     /translation="FATDLADPSKVIAEPSGVMIVPFGSERVGDVSNVVFTNGAIARD
                     NGDVFIYYASSDTRMHVATTTIDRLIDYVFHTPEDPLRSVECVAQRCELIKKNLEILK
                     REK"
     gene            381..>1275
                     /locus_tag="PLU43_11475"
     CDS             381..>1275
                     /locus_tag="PLU43_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOO29075.1"
                     /translation="MIIKADAPVLTYCGRIDFTKKEEPVFIYPYSSVSMVFTGKRISI
                     IVRNHHVCWENHLGYVIDGVQGRIRLPENAQEVTLLLADDLTEEEHELFLFKRQDACH
                     YFDLLGFVLEDGAEVRSSAPLPARKIEVYGDSVSAGEVSEAIDYVGKPDPVHNGEYSN
                     AYYSYAAYTARMLHAQLHDIAQGGIALLDHTGYFAPAFGYIGMENRFDKLKYNPELGE
                     VNTWDFSRYTPHVVVVAIGQNDNYPEDYMQDDYRCEKAVEWRTVYERLLRDIREKYQK
                     ALIIAATTILEHDESWDRSIED"
BASE COUNT          359 a          249 c          345 g          322 t
ORIGIN      
        1 tttgccactg atctggcaga cccatcaaag gtcattgcgg aaccaagcgg cgttatgatc
       61 gtgccttttg gaagcgaaag ggtcggtgac gtatccaatg ttgtcttcac gaacggtgcg
      121 atcgcaaggg ataacggcga tgtattcatt tattatgcgt cttcggacac aaggatgcat
      181 gttgccacaa caacgatcga ccggctgatc gattatgtgt tccatacgcc agaagacccg
      241 cttcgctccg tggaatgtgt tgcacaaaga tgcgaactga tcaaaaaaaa tctggaaata
      301 ctgaaaagag aaaaatagaa agaaaagagt ccgatgcggg ctctttcttt caatactatc
      361 agcggaaagg aaaagagagc atgataataa aagcagatgc gccggtactt acatactgcg
      421 gcagaattga ttttacaaaa aaggaggagc cggtatttat ttatccatat agttcggttt
      481 ccatggtctt cacaggaaag cgtatcagta tcattgtaag aaatcatcat gtatgctggg
      541 agaatcatct tggttatgta atagacggcg tacagggcag aatacgcctt cctgaaaatg
      601 cacaggaagt gacgctgctt cttgcagatg atctgacgga ggaagaacat gaactgtttt
      661 tgtttaaacg ccaggatgca tgccattatt ttgacctgct tggttttgta cttgaagacg
      721 gcgcagaggt cagaagctcc gcaccgcttc cggcaagaaa gatcgaagta tatggggact
      781 cggtatcggc aggagaggtg tcggaagcga ttgactatgt tggtaaaccg gatccggtcc
      841 ataatgggga atattccaat gcttactatt cctatgcggc ttatacggcg cgaatgctgc
      901 atgcacagct gcatgatatt gcgcagggcg gcattgccct tttggatcat accggttatt
      961 ttgcaccggc ctttgggtat atcggaatgg agaaccggtt tgataaatta aaatacaatc
     1021 cagagctcgg ggaagtcaat acatgggatt tttcccgtta tacaccgcat gttgtagttg
     1081 ttgccattgg gcagaatgac aattatccgg aggattatat gcaggatgat tatcggtgtg
     1141 aaaaagcggt ggaatggcgg actgtgtatg agcgtctgct gcgggacatc cgggaaaagt
     1201 atcaaaaggc gctgatcatt gcggcgacaa cgattctgga gcacgatgag agctgggatc
     1261 gatccattga ggacg
//
LOCUS       DAOSYE010000781         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99432_length_1274_cov_2.957342, whole genome shotgun sequence.
ACCESSION   DAOSYE010000781 DAOSYE010000000
VERSION     DAOSYE010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99432_length_1274_cov_2.957342"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1274
                     /locus_tag="PLU43_11480"
     CDS             <1..>1274
                     /locus_tag="PLU43_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO29076.1"
                     /translation="FAAIGSAMNYHEDNFITLQEMKNKLSGNIKMEFEVARMDPLFAS
                     KEEFKEFETRHNAHCVSTGELDGYSGNCYLGIDAGSTTTKAALVGEDGSLLYSFYSNN
                     NGSPLATTIRAIKEIYSLLPANAKIAHSCSTGYGEALIKAALLLDDGEVETVAHYYAA
                     AFFEPDVDCILDIGGQDMKCIKIKNHTVDSVQLNEACSSGCGSFIETFAKSLNYSVTD
                     FAKAALYAEHPIDLGTRCTVFMNSKVKQAQKEGADVSDISAGLAYSVIKNALFKVIKV
                     SDASALGEKIVVQGGTFYNDAVLRSFEKIADCNAIRPDIAGIMGAFGAALIARERYAD
                     SRTTTMLPIEKINTLEFTTSMAKCKGCTNNCRLTINKFTGGRQYISGNRCERGLGKVK
                     NENNLPNLFEYKEKRIFDYEPLSLPDAKRGQV"
BASE COUNT          360 a          282 c          291 g          341 t
ORIGIN      
        1 gtttgctgct atcggctctg ccatgaatta tcatgaagat aattttatta cgttacagga
       61 aatgaaaaac aagctgtccg gcaatatcaa gatggaattt gaagtcgcgc gtatggaccc
      121 gctttttgcc tcaaaagaag aatttaagga attcgaaacc cgccataatg cccattgtgt
      181 ttccaccggt gaacttgatg gatattccgg caactgctat cttggtattg atgcgggatc
      241 gactacgaca aaagccgcac tggtcggtga agacggctcc ctgctctact ccttttacag
      301 caacaataac ggcagtccgc ttgcgactac gatccgtgcc atcaaggaga tctattccct
      361 gcttcctgca aatgcaaaaa ttgcccattc gtgctctacc ggatatggtg aagcgcttat
      421 aaaagctgcg ctccttttgg acgacggtga agttgagact gttgcgcatt attatgcagc
      481 tgcttttttt gaacctgacg ttgactgtat tctggatatc ggcggacagg atatgaaatg
      541 cattaaaatt aaaaatcata cggtggacag tgtacagctc aatgaagcct gctcttccgg
      601 ctgcggatca tttatcgaaa cctttgccaa atcattaaac tattctgtaa ctgattttgc
      661 aaaagctgcc ctgtatgccg agcacccgat cgatctcggc acacgctgca ctgttttcat
      721 gaattccaaa gtaaagcagg cacagaagga aggcgcagat gtttcggata tctcagcagg
      781 tcttgcttat tccgtcataa aaaatgcatt atttaaagtt attaaggttt ccgatgcatc
      841 agctttgggt gaaaagatcg ttgtacaggg cggcaccttt tataatgatg cggttctgcg
      901 cagttttgaa aaaattgcgg actgcaatgc gatccgcccg gatatcgcag gtattatggg
      961 tgcattcggt gcggctctga ttgcacggga acgctatgcc gattccagga ctaccacaat
     1021 gctgccgatc gaaaagatca atacacttga atttacaaca tccatggcca aatgcaaagg
     1081 atgtacaaac aactgccggc tcacgatcaa taaatttacg ggaggacggc agtatatttc
     1141 aggaaaccgc tgcgagcgtg gtcttggaaa agtaaaaaac gaaaataatc ttcctaatct
     1201 gttcgaatat aaagaaaaac ggatctttga ctatgaaccg ctttctcttc cggatgcaaa
     1261 acgcggtcag gtcg
//
LOCUS       DAOSYE010000782         1273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_99607_length_1273_cov_2.390805, whole genome shotgun sequence.
ACCESSION   DAOSYE010000782 DAOSYE010000000
VERSION     DAOSYE010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99607_length_1273_cov_2.390805"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLU43_11485"
     CDS             complement(<1..121)
                     /locus_tag="PLU43_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOO29077.1"
                     /translation="MVISVLSGKGGTGKTTVVAALSELARNMVKVDCDVDAPNL"
     gene            complement(115..942)
                     /locus_tag="PLU43_11490"
     CDS             complement(115..942)
                     /locus_tag="PLU43_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29078.1"
                     /translation="MNIAILSGKGGTGKTTVSTNLAVALGVSYVDCDVEEPNGFIFLD
                     PSIRKSEDVFVEYPVIDRETCVLCGECAKVCQFHALAKAGDEIVVFEKLCHDCGACRL
                     VCEAGALSYAKRPIGRLEEGPAKGIDCFRGSLFVGEPMAVPVIRKLLQELPEEDHLVD
                     CPPGTSCNVVTALKYANAAVLVTEPSEFGLHDVKMAVELVKLYQIPYGIVINKDNGKD
                     NMIKAFCRENDVPLFGTIPYSMEAAALYSKGIMLYEAHDHKENFIMIAQKIKEAFAW"
     gene            complement(962..>1273)
                     /locus_tag="PLU43_11495"
     CDS             complement(962..>1273)
                     /locus_tag="PLU43_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO29079.1"
                     /translation="RRFGRCEYFLIYDNETKEYKAVANKGVASSQGAGIAAASQVIDE
                     NVSAVITGNLGPNAFELIEKAGIKAYGCEAVPVYRAVEMFQKDQLTAVSFAGRAHQGM
                     R"
BASE COUNT          304 a          384 c          238 g          347 t
ORIGIN      
        1 acaggttcgg cgcatccaca tcacaatcga ccttcaccat attccgggcc agctcggaca
       61 gcgccgcaac aacagtcgtt ttcccggttc cgccctttcc gctcaacact gatattacca
      121 tgcaaacgcc tcctttatct tctgagcaat catgataaag ttctctttat gatcatgcgc
      181 ctcatacagc atgatccctt tggaatacag tgcggcggct tccatactgt aggggatcgt
      241 cccaaacaga ggcacatcat tttccctgca gaatgccttg atcatattat ctttgccgtt
      301 atctttatta ataacgatcc catatggaat ctgatacagc tttacaagct caacagccat
      361 ttttacgtca tgaagcccaa attcggacgg ctccgtcaca agaacagccg cattcgcata
      421 tttcagcgca gtaactacat tgcaggaggt tcccggcgga cagtccacaa gatggtcctc
      481 ttccggcagc tcctgcagga gcttgcgtat gaccggaact gccatcggct ctcctacaaa
      541 aagacttccg cgaaaacagt cgattccttt tgcaggacct tcctccagcc tgccgatcgg
      601 acgcttcgca taagacagcg ctcctgcctc acacacaagc ctgcatgcgc cgcagtcgtg
      661 gcacagcttc tcaaaaacaa cgatttcatc tcctgctttt gcaagtgcat gaaactggca
      721 gaccttcgca cattccccgc ataaaacgca cgtttccctg tcaatgacag ggtattccac
      781 aaatacatct tcactttttc ggatagaagg atccaaaaaa ataaatccgt tcggctcttc
      841 cacgtcacaa tcgacataac tgacgccaag tgctacggcc agatttgtag agactgtcgt
      901 ttttccggtt cctccctttc cgctcagtat tgctatgttc atatcattct cctctgcccc
      961 gtcatctcat tccctgatga gctcttcctg caaaggaaac agcggtcagc tgatctttct
     1021 ggaacatttc gaccgcccgg tataccggaa ccgcctcaca gccatatgct tttatgcctg
     1081 ccttttcgat cagttcaaat gcattcggtc ccagattacc ggtgatgact gccgatacat
     1141 tttcatctat cacctggctt gccgccgcga ttcctgctcc ctggctcgaa gcgactcctt
     1201 tatttgcgac agctttatac tcctttgttt cgttatcata gatcagaaaa tattcacatc
     1261 ttccaaatct tct
//
LOCUS       DAOSYE010000783         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_100628_length_1265_cov_2.901653, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000783 DAOSYE010000000
VERSION     DAOSYE010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100628_length_1265_cov_2.901653"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLU43_11500"
     CDS             <1..251
                     /locus_tag="PLU43_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOO29080.1"
                     /translation="WIRESTKKQSWCEDWGGLAYGYLWWIIDEKEPAYAAMGDGGNVI
                     YVNVKKKLVISIASLFMPLAEDRIKLIREYMEPLFEKM"
     gene            285..1154
                     /locus_tag="PLU43_11505"
     CDS             285..1154
                     /locus_tag="PLU43_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOO29081.1"
                     /translation="MRIQAYTLNSFAKTEEGGNPAGVVLHADSLSEETMRKIAAVLGF
                     SETAFVMQSDAADFKVRFFTPNEEVDLCGHATIAVFHVMSRLNLLNPGRYTQETKAGL
                     LGVEIQKDHYIMMEQTAPVFSEMIAPCEIADSLRISSSRIPKELPVQVVSTGLRDIMI
                     PVSSVDILHAISPNMELVEKLSRKYNTVGYHVFSLEALQDMGVYCRNFAPLYGIPEEA
                     ATGTSNGALACYLYHCGQINEKQAADLVFCQGYSMKKPSEIRASLTVKDHEICMVKIG
                     GSAMNYSVKELTL"
BASE COUNT          364 a          219 c          331 g          351 t
ORIGIN      
        1 gctggattag ggaaagtaca aagaaacaaa gctggtgtga agactggggt ggactggcat
       61 atggttattt gtggtggatc atcgatgaga aggaaccggc ctatgcagcg atgggggacg
      121 gtggaaatgt gatctatgtc aatgtgaaga aaaaactggt gatctccatt gcctctcttt
      181 ttatgcccct tgcagaggac aggatcaaac tgatcagaga gtatatggaa ccactgtttg
      241 agaagatgta gaattgtgaa ataaaattta caggagtcaa aaatatgaga atacaggcat
      301 acacgttaaa ttcttttgca aaaacagagg aaggaggaaa tccggcaggt gtagtgctgc
      361 atgcggattc cttatcggaa gagacaatga gaaaaattgc agctgtgctt ggattcagtg
      421 aaactgcctt tgtgatgcag tcagatgcag ctgattttaa ggtgagattt tttacgccga
      481 atgaggaagt ggatctgtgc ggccatgcta cgattgctgt ttttcatgtg atgtcacgtc
      541 tgaacttgtt aaacccgggc agatatacgc aggaaaccaa ggccgggctc cttggggttg
      601 aaatacaaaa ggatcattat attatgatgg aacagacggc gcctgtattc tcggaaatga
      661 tagctccgtg tgagattgcg gattccctgc gtatcagttc ttcccggata ccaaaggagc
      721 tgccggtgca ggtcgtatcg acaggactcc gtgatataat gattcccgta agtagcgtgg
      781 acatactgca tgcgatcagt cccaatatgg aactggtcga aaagctcagt cggaaatata
      841 acaccgtcgg atatcatgta ttttctttgg aagcattaca ggatatgggg gtttactgca
      901 gaaattttgc acccctgtat ggaattccgg aagaggctgc aacgggaact tcaaatgggg
      961 cgcttgcctg ttatttatat cattgcggac agatcaatga aaagcaggcg gccgatcttg
     1021 ttttttgtca gggatattcc atgaaaaagc cgtctgagat ccgtgcatcg ctgacagtaa
     1081 aagatcatga gatctgtatg gtaaaaatag gaggaagtgc aatgaattac agtgtaaagg
     1141 agcttacact gtaattcatt gcacttcctt tcattgtttg gagaagatct tttcgttatt
     1201 ttttgtcatc tcttcgatga ttgcttctat ttcatcaaat aaagagatca tttcttttga
     1261 gaagg
//
LOCUS       DAOSYE010000784         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_100936_length_1263_cov_2.677980, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000784 DAOSYE010000000
VERSION     DAOSYE010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100936_length_1263_cov_2.677980"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLU43_11510"
     CDS             <1..1242
                     /locus_tag="PLU43_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29082.1"
                     /translation="VVDWFITLAVVVILGVFTVLYLLLFKNKAREYGKENQIYDGKMH
                     QSINQALGAVKDIKILHREKYFVNAFTGYGKKKMHAVRNNNVLGQAPKYIIETVCIGA
                     VLLVLVFKIYQGEDLKTMIPQLAAFAVAAFKLLPSVSKINNFANLIIFLKPSVDLIYR
                     DIKDTEDMKDFELQDASVSDEVYADNHPSCIGISHISYRYPHTDRDVLHDVSFEIPLG
                     AAVGLIGPSGAGKSTMADVILGILTPTEGAINYGVMNVHEHPLKWSKKLAYIPQAIYL
                     ADETIRSNVAFGIDEELIDEEKVWSALAEAQLMEFVKELPQGLETMVGERGVRLSGGQ
                     RQRIGIARALYDNPEILVLDEATSALDSGTEAAVMDAIDRLQGRKTLIIIAHRLTTIQ
                     NCQFVFKVENGTIQSVDPASL"
BASE COUNT          339 a          254 c          345 g          325 t
ORIGIN      
        1 gtggtggact ggttcattac attggcagtc gttgtgatcc ttggcgtatt taccgttttg
       61 tatctgcttt tgtttaaaaa taaggccagg gaatatggaa aggaaaatca gatctatgac
      121 ggaaagatgc atcagtcgat caatcaggcc cttggtgctg tcaaggacat taagattctt
      181 cacagggaaa aatattttgt aaatgcattt accggctatg gaaagaaaaa gatgcacgcg
      241 gtccgcaata ataacgtgct cggacaggcg cccaaatata ttattgaaac ggtctgtatc
      301 ggtgcggtgc ttcttgtgct tgtatttaag atctatcagg gggaggatct caagacgatg
      361 atccctcagc tggcggcatt tgccgtggca gctttcaagc ttctgccgtc ggtcagcaag
      421 atcaataatt ttgccaatct gatcattttt ctgaagccgt ctgtggatct gatctatcgt
      481 gatatcaagg atacggagga catgaaggac tttgaactgc aggatgcgtc ggtttcagac
      541 gaggtctatg cagacaatca tccgtcctgt atcgggatct ctcatatttc ctatcgatat
      601 ccgcatacgg acagggatgt gttgcatgat gtttcttttg aaattccgct tggcgctgcc
      661 gtagggctca tcggtccgtc aggtgcgggt aaatcgacaa tggcggatgt gattttggga
      721 attctgacgc cgacagaagg agccattaac tatggggtga tgaatgtaca cgaacatccg
      781 ctcaagtggt caaagaaact tgcctatatt ccgcaggcga tctatctggc cgatgaaacg
      841 atacggagca atgtggcgtt tggcattgac gaagaactga tcgatgaaga aaaggtctgg
      901 tcagcacttg cagaagcaca gctcatggag ttcgtaaaag agcttccgca gggtttggag
      961 acaatggtcg gtgagcgagg tgtacggctt tcgggaggac agcgccagcg tatcggcatt
     1021 gcaagggcgc tttatgacaa tcctgaaatt cttgtgctcg acgaggcgac ttctgcgctc
     1081 gattccggaa cggaagcagc ggtcatggat gcgatcgaca ggctgcaggg aagaaaaaca
     1141 ctgatcatca ttgcgcatcg tctgacaacg atccaaaact gccaatttgt atttaaggtg
     1201 gaaaacggaa caattcaaag tgtggatccg gcatccctgt aagaaaaagt ataaaaagaa
     1261 ggg
//
LOCUS       DAOSYE010000785         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_101347_length_1260_cov_3.073029, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000785 DAOSYE010000000
VERSION     DAOSYE010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101347_length_1260_cov_3.073029"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLU43_11515"
     CDS             complement(<1..577)
                     /locus_tag="PLU43_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29083.1"
                     /translation="MKKDSLFHKICRIVLKSAVAVFLVIVLNLIFIPKYIHENQDGRI
                     TEEFYKEQLDTDIIFVGSSTVYSGVSPLILWEKYGYTSYDRSNSSQTIWISYYMIEDA
                     IKCHKPKLVVLDIGFIKYWDTYAEEPSNRKAIDGMRFSKTKLACIGAAMDQDEKITDY
                     LFPILRFHSRWKELQPEDFLYDLCYCMCLIYL"
     gene            complement(587..>1260)
                     /locus_tag="PLU43_11520"
     CDS             complement(587..>1260)
                     /locus_tag="PLU43_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOO29084.1"
                     /translation="HITLGTWFKDYIFYPLSIAKPIMKLGKWTRTHVSQPLGKRIPIY
                     LPMIVVWAATGMWHGSENRYVVWGLLNCVFIILGTEFEPVSKWLIKKFHVNEKGLVLK
                     TFRIVKTFWLMAFLRIFDISKNVDAAFETMKYMFMEIPSFRLSAVFADLNLPAEEFAV
                     ACAAILILFCVSMLQRSGSLRERIFRLPIPLQWCVLSALVGAVVIFGFYGRGFDAQQF
                     IYLQF"
BASE COUNT          398 a          316 c          220 g          326 t
ORIGIN      
        1 caagataaat caggcacata cagtaacaaa ggtcataaag aaaatcctct ggctgcagtt
       61 ctttccagcg tgaatggaat cgtaaaatcg ggaacagata atccgtaatt ttttcatcct
      121 gatccattgc cgcaccaata caggcaagtt ttgtttttga gaaccgcata ccgtcgatcg
      181 ctttcctgtt ggagggttct tccgcataag tatcccaata cttgataaat ccgatatcaa
      241 gtacaaccag cttcggttta tgacatttga tcgcatcttc gatcatataa taggaaatcc
      301 aaatcgtctg cgacgaatta ctccggtcat aagaagtata gccatatttt tcccataaga
      361 tcagcggcga cacaccactg tacacggtcg aagaccccac aaaaataata tccgtatcca
      421 gctgttcctt ataaaattct tctgtgatcc ttccgtcctg attttcatga atatactttg
      481 gtataaatat cagatttaaa acaatgacaa gaaagactgc aactgccgat ttcaaaacaa
      541 tccggcagat tttatgaaac aaagaatctt ttttcatatc tgcctcctaa aactgcaggt
      601 aaataaactg ttgggcatca aagccccgcc catagaatcc aaaaatcaca accgccccaa
      661 ccagcgcaga aagcacacac cactgcaggg gaataggcag cctgaatatc ctttcacgca
      721 ggctccctga tctttgcagc atactgacgc agaacaaaat caagatcgcc gcgcaggcta
      781 cggcaaattc ctctgccgga agatttaaat ctgcaaaaac agcactgagg cgaaacgacg
      841 gtatttccat aaacatatat ttcattgttt caaatgccgc atccacattt ttcgaaatat
      901 caaaaatacg aagaaatgcc atcagccaga acgtttttac aatacgaaac gttttcagca
      961 caagcccttt ttcattcaca tgaaattttt tgatcagcca cttggaaacc ggctcgaact
     1021 ccgtgccgag aatgataaat acacagttca gcagccccca tacgacatat cggttctcag
     1081 agccgtgcca cattccggta gcagcccata cgacgatcat cggcaaatag atcggtatcc
     1141 tttttccaag cggctgactc acatgcgttc ttgtccattt ccccagtttc atgatcggct
     1201 tcgctatgga cagcggataa aaaatataat ctttaaacca tgttccaagc gtaatatgcc
//
LOCUS       DAOSYE010000786         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_101364_length_1260_cov_2.654772, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000786 DAOSYE010000000
VERSION     DAOSYE010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101364_length_1260_cov_2.654772"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1260)
                     /locus_tag="PLU43_11525"
     CDS             complement(<1..>1260)
                     /locus_tag="PLU43_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOO29085.1"
                     /translation="LEPEVYYDLVDKIAIRKSVREKYVQEIVDEVSRHIKNAGIEANI
                     DGRVKHFFSIYKKMLNQDKTIDQIYDLFAIRIIVATVKDCYAALGVIHEMYKPIPGRF
                     KDYIAMPKPNMYQSLHTTLIGPTGQPFEIQIRTYEMHRTAEYGIAAHWKYKEASDGKR
                     VEEQEAEKLSWLRQILEWQKDMSDNKEFMNTLKSDLDLFSDSVYCFTPTGDVKTLPAG
                     SNTIDFAYSIHSAVGNKMIGARVNGKLVTIDYIINNGDRIEIITSQNSKGPSRDWLGI
                     VKSTQAKSKISQWFRNELKEDNIVKGKEMIAAYCKAKMIDTNQVIKPEYIHAIMDKYG
                     FKDWESVCAAVGHGGLKEGQILNKMQELYEKDHPKILSDEEVLQNVADTQKDKKIRVK
                     SKGGIVVKGIDDVAVRFSKCCSPIPGD"
BASE COUNT          289 a          337 c          234 g          400 t
ORIGIN      
        1 catctcccgg aataggactg cagcacttcg aaaaccgaac cgcaacatca tcaattcctt
       61 tcacaacgat accgcccttg ctcttgacgc gtattttttt atctttctgt gtatccgcaa
      121 cattctgcag gacttcttca tcggaaagga tcttcgggtg atccttttca tacagctcct
      181 gcattttgtt gaggatctgc ccttccttca gaccgccgtg ccctacggct gcgcagacag
      241 actcccagtc cttaaaccca tatttatcca tgatcgcatg aatatattcc ggcttgatca
      301 cctggtttgt atctatcatc tttgccttgc aataggcagc gatcatttct tttcctttaa
      361 caatattatc ttccttcagt tcattcctga accactggct gatcttgctt tttgcctgcg
      421 tacttttaac gataccgagc cagtccctgc tcggtccttt tgaattttgg gaagtgatga
      481 tctctatccg gtcgccgtta ttgataatat aatcgatcgt gaccagcttt ccgtttacac
      541 gggccccgat catcttattg ccgaccgcac tgtgaatact gtaagcaaaa tcgatcgtat
      601 tggaaccggc aggcagtgtc ttgacatctc ctgtcggcgt aaaacagtat acgctgtccg
      661 aaaacagatc cagatcgctt ttcagcgtgt tcatgaattc cttattatcc gacatatcct
      721 tctgccactc caggatctgt ctaagccagc ttaacttttc ggcttcctgc tcctcgactc
      781 tcttgccgtc ggacgcttct ttatatttcc aatgcgcagc aataccatat tctgccgtcc
      841 tgtgcatttc atacgtacgg atctgtattt caaacggctg acccgtcgga ccgatcagtg
      901 tcgtatgcag tgactggtac atatttggtt tcggcatcgc aatataatcc ttaaaacgtc
      961 ccggaatcgg tttatacatc tcatgaataa cgccaagcgc tgcgtagcag tccttgactg
     1021 tggctacaat aatccggatc gcaaacagat catagatctg gtcgatcgtt ttatcctgat
     1081 tcagcatctt tttatagata ctgaaaaaat gttttaccct gccgtcaatg tttgcttcaa
     1141 taccggcatt tttaatatgt ctggaaactt catccacgat ctcctggaca tatttttccc
     1201 gcacgctttt tcgaatggcg atcttatcga caagatcgta atacacttcc ggctcaagat
//
LOCUS       DAOSYE010000787         1259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_101550_length_1259_cov_2.274917, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000787 DAOSYE010000000
VERSION     DAOSYE010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101550_length_1259_cov_2.274917"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLU43_11530"
     CDS             <1..220
                     /locus_tag="PLU43_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HOO29086.1"
                     /translation="KQLMPVSNPLFSLIRFLWHENEKMLQIMPREGTYDGMQTERDFI
                     IEVLGRQIKTLHYTGSELSVLIHDNPAD"
     gene            complement(186..935)
                     /locus_tag="PLU43_11535"
     CDS             complement(186..935)
                     /locus_tag="PLU43_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29087.1"
                     /translation="MKYGIVVAEDEALLLENLIQKIEAADLGFEVIGSAQTGIQAYEL
                     VEELNPDVLITDIRMPAMDGLSLIQKINESHPRMDCIITSGYSDFEYARTALKYHVYD
                     YLLKPVDIEELKTVLKKLDNKYTLEQNALEDIFRNTAESTPSEIAALLKEYIIQNFNE
                     EINLNLIAHNMNYSSSYLTKIFCQYYDCTPSKYIISLRMQKAQQLLSHHTELSIRQIG
                     EAVGYPEQGYFSRIFKKQTGVSPLDYRESKH"
     gene            complement(932..>1259)
                     /locus_tag="PLU43_11540"
     CDS             complement(932..>1259)
                     /locus_tag="PLU43_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO29088.1"
                     /translation="AIKYGTDCIPPWFITIKGTCENDRWTITVTDSGDGFSEDTLIML
                     HKKIEETTLNSGMPELTINGLGLLNVYMRWKIYMKDKAVFSFGNTENGHAYVSIGASG
                     KKEDSL"
BASE COUNT          312 a          268 c          303 g          376 t
ORIGIN      
        1 aaagcagctg atgccggttt cgaacccatt gttttccctg atccgatttt tatggcatga
       61 gaatgagaaa atgctgcaga tcatgccaag agaaggcact tatgacggga tgcagacaga
      121 aagggatttt atcatagagg ttcttggaag acaaatcaaa acactgcatt atacgggaag
      181 tgaactcagt gttttgattc acgataatcc agcggactga cgccggtctg ttttttaaag
      241 atccggctga aatatccctg ctcaggatag cccacggctt caccgatctg acggatggaa
      301 agctccgtat gatgggagag aagctgctgg gctttttgca tgcgcaggga aataatatat
      361 ttggaaggcg tacagtcgta atactgacag aagatctttg tcagatagct ggagctgtaa
      421 ttcatgttgt gggcgatcag gttcaaattg atttcttcgt taaaattctg gatgatatat
      481 tccttcagca gagccgctat ttcagaaggt gtactttctg cggtgttacg gaaaatatct
      541 tccagtgcgt tttgttccag cgtatatttg ttgtccagct tttttaatac tgtttttaat
      601 tcctctatgt ccacgggctt caacagataa tcatatacat gatatttgag tgcggtgcgg
      661 gcatattcga aatccgaata gccgcttgta atgatgcagt ccatccgtgg atgggattcg
      721 tttattttct ggatcaggga aagtccgtcc atggcaggca tgcggatatc cgtgatgagt
      781 acgtccggat tcagttcttc gaccagttca taggcctgga tacctgtctg tgcggagccg
      841 atcacttcaa agccaaggtc ggcggcttcg atcttctgaa taagattttc taacagcagg
      901 gcttcgtctt ccgctacaac aataccgtat ttcataatga atcctccttt tttccgcttg
      961 caccgatcga aacataggca tgtccgtttt ccgtattgcc gaaagagaag acggctttat
     1021 ctttcatata gattttccag cgcatataga cgtttaacag gccgagaccg ttgatcgtca
     1081 gttccggcat gccggaattc aaggtcgttt cctcgatttt tttatgcagc attatcagcg
     1141 tgtcctctga aaagccgtct ccggagtcgg taacagtgat cgtccagcga tcgttttcac
     1201 aggtgccctt tatcgtgatg aaccatggcg gaatacagtc tgtgccatat ttgatcgcg
//
LOCUS       DAOSYE010000788         1259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_101552_length_1259_cov_2.254983, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000788 DAOSYE010000000
VERSION     DAOSYE010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101552_length_1259_cov_2.254983"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLU43_11545"
     CDS             complement(<1..283)
                     /locus_tag="PLU43_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29089.1"
                     /translation="MAQIQLLDALTINHIAAGEVVERPMSVVKELIENAIDAGADAIT
                     VEIKEGGIDYIRVTDNGCGIEKSQVPTAFLRHATSKLHSVEDLLSIKSLG"
     gene            complement(325..>1259)
                     /locus_tag="PLU43_11550"
     CDS             complement(325..>1259)
                     /locus_tag="PLU43_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO29090.1"
                     /translation="RPKINEKGSIEIKDGRHPVVENMIASDMFVANDTYLDNGNNRIS
                     IITGPNMAGKSTYMRQTALIVLLAQIGSFVPAKSADIGIVDRIFTRVGASDDLASGQS
                     TFMVEMTEVANILRNATARSLLILDEIGRGTSTFDGLSIAWAVIEYISNPKLLGAKAL
                     FATHYHELTELEGKLKAVNNYCIAVKEQGDNIVFLRKIVKGGADKSYGIQVAKLAGIP
                     DIVIERAKELVEELSENDITASVKSIAEESKGMKKHPSPKFDEVDLAQISLFDTVKDD
                     DIVTELKELDLNNLTPMEALNTLCRLQNRLKNRW"
BASE COUNT          307 a          319 c          252 g          381 t
ORIGIN      
        1 aaccaagact ttttatggaa agcagatcct ccacagaatg gagcttactt gttgcatgac
       61 gtaaaaatgc cgtgggcacc tgtgattttt caataccgca gccgttgtcc gtcacacgga
      121 tataatcaat accgccttcc ttgatttcga cggtaattgc atctgccccg gcatcgattg
      181 cattttcaat cagttccttg acaacagaca ttggccgctc aacaacttct ccggcagcaa
      241 tatgattaat tgtaagcgca tccagtaatt gaatttgtgc catgttcttc tcctcttctt
      301 ataagccggt ctgctatacc cgggtcacca gcggtttttc agcctgttct gcagtctgca
      361 aagcgtattc agtgcttcca ttggcgtcag attgtttaaa tcaagctctt ttaattccgt
      421 aacgatatca tcgtccttta ccgtgtcaaa taatgatatt tgtgccagat ccacttcgtc
      481 aaatttgggg gacggatgtt ttttcatacc cttgctttct tccgcaatac ttttgacact
      541 tgcggtaata tcattttcag aaagctcttc aacaagctct tttgcacgtt caataacgat
      601 atcgggaatc cccgcaagct ttgccacctg aataccatag cttttgtcgg caccgccttt
      661 tacgatcttt ctgagaaata cgatattatc tccctgttct ttaactgcaa tacaataatt
      721 gttgactgct ttcagttttc cttccagttc cgttaattca tgataatgtg tcgcaaacag
      781 cgcctttgcc ccaagcagtt tcggattgct gatatattcg ataactgccc atgcgatcga
      841 aagaccgtca aaggtcgagg tgccgcggcc gatctcatca aggatcagca ggctccttgc
      901 cgttgcattt ctcaaaatat tagcaacctc tgtcatctcg accataaagg tgctctgccc
      961 gcttgccaga tcatcggaag caccgaccct tgtaaagata cggtctacga taccgatgtc
     1021 ggcgcttttg gcaggtacaa aactgccgat ctgcgcaagc agaacgatca gggcggtctg
     1081 ccgcatatag gtggatttac cggccatatt cggaccggtg ataatagaga tccggttatt
     1141 tccattgtcc agatacgtat cgtttgcaac gaacatatcg gaagcaatca tattttcaac
     1201 aaccgggtgg cgtccatctt tgatctcaat ggatcctttt tcattgatct tcggacgac
//
LOCUS       DAOSYE010000789         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_101947_length_1256_cov_3.220649, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000789 DAOSYE010000000
VERSION     DAOSYE010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101947_length_1256_cov_3.220649"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLU43_11555"
     CDS             complement(<1..366)
                     /locus_tag="PLU43_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO29091.1"
                     /translation="MLRIAVLVSGGGTNLQAIIDAVKQRKLTAVEIALVISNKPDVFA
                     LERAKTCEIPAICINPKEYADKEAYERAMLDALSKADAELIVLAGFLVVLSPGFVKAY
                     EGRIINIHPSLIPSFCGDGY"
     gene            complement(360..>1256)
                     /gene="purM"
                     /locus_tag="PLU43_11560"
     CDS             complement(360..>1256)
                     /gene="purM"
                     /locus_tag="PLU43_11560"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOO29092.1"
                     /translation="SLEKYKNMEKPTLVSGTDGVGTKLKLAFLLDKHETIGIDCVAMC
                     VNDIACAGGEPLFFLDYIACGKNEPEKIALIVKGVAEGCRQAGAALIGGETAEMPGFY
                     PEDEYDLAGFAVGIVDEKDLISGREIREGDAIIGIASSGIHSNGYSLVRKVFPMRESC
                     ITEYVSSLGCTLGEALLTPTKIYVKALKSVKEAGIRIKGCSHITGGGFYENIPRMLPD
                     GMRAVIKKDSYQVLPIFRLLQESGNIREEMMYQTYNMGIGMMLAVDSADAERTIKALE
                     QAGETAYRVGTIEAGEKGIQLC"
BASE COUNT          300 a          364 c          235 g          357 t
ORIGIN      
        1 atacccatca ccgcaaaaag aaggaatcag cgacggatga atattgatga tcctgccctc
       61 atacgccttt acaaagccgg gagaaagaac tacaagaaaa ccggccagca cgatcagttc
      121 tgcatctgct tttgacaatg catcgagcat tgcacgttca tatgcttctt tgtccgcata
      181 ttcctttgga ttgatgcaga tcgccggaat ttcacatgtt ttggctcgtt ccagcgcaaa
      241 aacatccggt ttattactaa taacaagtgc tatttcaaca gcagtcagct ttctttgttt
      301 gactgcatct ataatcgcct gcagatttgt accgccgccc gataccagta ccgctatcct
      361 taacataact gtataccctt ttcaccggct tcgatcgttc ctacccgata tgccgtttct
      421 cctgcctgtt caagcgcttt gatcgtacgt tcggcatcag cgctgtctac tgcaagcatc
      481 atgccgattc ccatattata tgtctggtac atcatttcct cacggatatt gccgctttcc
      541 tgcagcagcc tgaatatggg aagtacctga taggagtctt ttttgattac tgcacgcata
      601 ccgtccggaa gcatccgtgg tatattttca taaaaaccgc cgcccgtaat atggctgcag
      661 cccttgatcc tgattccggc ttctttcaca ctctttaacg ccttcacata aattttggtc
      721 ggcgtgagca gtgcttctcc caatgtacat ccaagacttg atacatattc cgtaatacag
      781 gattccctca tcggaaacac tttccgcaca agagaatacc cgttgctgtg tatgcctgaa
      841 gaagcgatcc cgataatcgc atcgccttcc ctgatctccc tgccggaaat cagatccttc
      901 tcatctacga tccctaccgc aaaacctgcc agatcatatt catcctccgg ataaaatcca
      961 ggcatttccg ctgtttctcc gccgatcagg gctgcgccgg cctgtctgca gccttccgcc
     1021 acgcctttca cgatcagggc gattttttcc ggttcgtttt tcccgcatgc gatataatcc
     1081 agaaaaaaca gcggttctcc tcccgcacag gcaatatcat ttacacacat cgccacgcag
     1141 tcaattccga tcgtctcatg cttatccaaa agaaatgcca gtttcagctt tgtgccgaca
     1201 ccgtcagtcc ctgatacgag cgtcggcttc tccatatttt tatacttttc caatga
//
LOCUS       DAOSYE010000790         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102025_length_1256_cov_1.748543, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000790 DAOSYE010000000
VERSION     DAOSYE010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102025_length_1256_cov_1.748543"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLU43_11565"
     CDS             complement(<1..453)
                     /locus_tag="PLU43_11565"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOO29093.1"
                     /translation="MIKKLYGSKKPVFSFEVFPPKKEEELAAVYGTLDELAALSPDFI
                     SVTFGAGGGNSKKSAEVASYIQNTCHVEALLHMTCVGFKRRELEESLHLLEENNLHNL
                     LALRGDRPQSMTDEQYNSRDFIYANEMILYIREHYKKSLCIGAACYPEK"
     gene            568..>1256
                     /locus_tag="PLU43_11570"
     CDS             568..>1256
                     /locus_tag="PLU43_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29094.1"
                     /translation="MYKVMIVDDNMTNLIMAKKALEEYYEIVPVSSGQTALEFLKDMP
                     EPPDIILLDVDMPEVNGFYVISEIKNCPKLAEIPVIFLTAQDDPTTEVEGYYLGAADY
                     IKKPYTTVLLRKRLDVHVQFLESERKLKKYNDSISNTLREKVHNIVELEYAIVEMFVS
                     LMSNRSHIIAEHSMRVERYMTLYIDEVIKSRQFAVNEEDAEILVFASKMHDVGKICMT
                     DRCLADMGDLSL"
BASE COUNT          366 a          233 c          293 g          364 t
ORIGIN      
        1 tttttccgga taacaggctg caccgataca aagagatttt ttgtaatgtt cacggatata
       61 caggatcatt tcgtttgcat aaataaaatc tctgctgtta tactgttcat ccgtcatgga
      121 ctgcgggcgg tcgccgcgca gagcaaggag attatggaga ttattttcct ccagcagatg
      181 cagtgactct tctaattccc tgcgtttaaa accaacacat gtcatatgca gcagtgcttc
      241 gacatggcag gtattctgaa tataggaagc gacttcggcc gattttttgg aattgcctcc
      301 gccggctccg aaggtaacac tgataaaatc aggagaaagt gctgcaagtt catcaagagt
      361 gccgtataca gcagcgagtt cctcttcttt tttaggagga aaaacttcaa aggaaaaaac
      421 cggtttttta ctgccatata attttttgat cataggagca cctcacatta ttttcttgct
      481 tttcatactg gaatatcgta acataatatt gtagtatttt gcaaggaata tctgaaatgg
      541 cagtggaccg caaaacaggt gaaacaaatg tataaagtaa tgattgttga tgacaacatg
      601 accaatctga tcatggcaaa aaaggcgctg gaggagtatt acgaaattgt tccggtatca
      661 tccggacaga cggcgcttga atttttaaag gatatgccgg aaccgccgga tattattctg
      721 ctggatgtgg atatgcctga agtaaatggg ttttatgtaa tcagtgagat caagaactgt
      781 ccgaagcttg cggaaattcc agttattttt ttgacggcac aggatgatcc gactacggag
      841 gtagaggggt attatctcgg tgcggcagat tatattaaaa agccgtatac gaccgtactc
      901 ttacgaaaac gtctggatgt gcatgtacag tttctggaat ctgagcggaa gctgaaaaaa
      961 tataatgatt ctatctccaa tacacttcgc gaaaaagttc ataatatcgt ggaactggaa
     1021 tatgcaatcg tggaaatgtt tgtttccctg atgtccaacc gttcccatat tattgccgag
     1081 cattccatgc gggtggaacg gtatatgacg ctttacattg atgaggtcat aaaatcgagg
     1141 cagtttgccg ttaatgagga ggacgctgaa atccttgtat ttgcatccaa aatgcatgat
     1201 gtgggtaaaa tctgtatgac cgacagatgt cttgccgata tgggggattt aagcct
//
LOCUS       DAOSYE010000791         1255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102086_length_1255_cov_3.190000, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000791 DAOSYE010000000
VERSION     DAOSYE010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102086_length_1255_cov_3.190000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLU43_11575"
     CDS             <1..417
                     /locus_tag="PLU43_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29095.1"
                     /translation="LLRLWQKNGIQAWDIPPASALKTSIETITEYIDLHSSELLRVET
                     LAVMCHMSYSYFARSFKQIYGKSCKEYIEFVKICKADDLLLFTDHTLNYISQETGFTD
                     CSHFIKTYKKIKGITPKQRRMRHLSDAASSFMHDSR"
     gene            complement(398..817)
                     /locus_tag="PLU43_11580"
     CDS             complement(398..817)
                     /locus_tag="PLU43_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO29096.1"
                     /translation="MTITEASEKYELTKDTLRYYERIGLVPPVPRTRSGIRNYDEESC
                     RWIEFIKCMRGAGMQIEALIEYVNLAQQGDDTLFERKKLLLEQREILLKKQEEIGITI
                     SRLNYKIENYDKLLTKSNASQEESRHGRNSAVSGCHA"
     gene            complement(855..>1255)
                     /locus_tag="PLU43_11585"
     CDS             complement(855..>1255)
                     /locus_tag="PLU43_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO29097.1"
                     /translation="GVTMILSGMSDMEQLMQNMDTFSVEQPLSTGEMKVLAEITDNMA
                     DILPCTACRYCTDHCPQKLDIPVLLSIYNESRFSMTFLTSMAVDGLGKGKGPDACIGC
                     RSCEAVCPQQLKISEAMADFTLRLSQAQNQ"
BASE COUNT          319 a          323 c          236 g          377 t
ORIGIN      
        1 cttctaaggc tttggcagaa aaatgggata caggcatggg atattcctcc tgcttccgca
       61 ttgaaaacaa gtattgaaac gatcacggaa tacattgacc tgcattccag cgaattgctt
      121 cgtgttgaaa cgctcgctgt tatgtgtcat atgagttact cctattttgc cagaagcttt
      181 aaacagatct atggaaaatc atgcaaggaa tatattgaat ttgttaagat atgcaaagca
      241 gacgacctgc tgctttttac ggaccatacg ctgaattaca tcagtcagga gaccggattt
      301 acggattgtt cgcattttat taaaacatat aaaaaaataa aagggatcac ccccaaacaa
      361 agaagaatgc ggcacctgtc cgatgccgca tcttctttta tgcatgacag ccgctgaccg
      421 cactgttcct tccatgccgt gattcttcct gcgatgcatt gctctttgta agcaatttat
      481 cataattttc tattttataa ttaagcctgc tgattgttat gccgatctcc tcctgttttt
      541 tcaaaaggat ctctctctgc tcaaggagca gctttttcct ctcaaacagc gtatcgtctc
      601 cctgctgcgc cagattgaca tattcgatca gcgcctcaat ctgcatgccg gcacccctca
      661 tacatttgat gaactcgatc cagcggcagg actcctcatc ataattcctg attccgcttc
      721 tcgttctcgg aaccggcgga accagtccga tccgctcata atatctaagt gtatcttttg
      781 tgagttcata cttttcactg gcttctgtta tagtcataag cttcctccct gcaatttctg
      841 ttattgcatt tcctttattg attctgtgcc tgacttaaac gcagcgtaaa atcagccatt
      901 gcttccgata tctttaactg ctgcgggcat accgcttcac agctcctgca tccgatacac
      961 gcatccggtc ctttcccttt tccaagtcca tccactgcca tagaggtcag gaacgtcatg
     1021 ctgaaacggc tttcattata tatggaaagc aataccggaa tatccagctt ctgaggacaa
     1081 tgatccgtac aatacctgca cgctgtgcat ggcagaatat ccgccatatt atccgtgatc
     1141 tctgccaaaa ctttcatttc tccggtgctc agcggctgct ccactgaaaa ggtatccatg
     1201 ttctgcataa gctgttccat atccgacatg ccggaaagga tcattgtcac acccg
//
LOCUS       DAOSYE010000792         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102371_length_1253_cov_3.419866, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000792 DAOSYE010000000
VERSION     DAOSYE010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102371_length_1253_cov_3.419866"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1220
                     /gene="asd"
                     /locus_tag="PLU43_11590"
     CDS             135..1220
                     /gene="asd"
                     /locus_tag="PLU43_11590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO29098.1"
                     /translation="MSEKLKVGILGGTGMVGQRFISLLADHPWFEITVIAASERSAGK
                     TYEEAVGHRWKIACSMPESVKKIIVMNVNEVEAVASKVDFVFSAVDMTKEEIRAIEEA
                     YAKTETPVVSNNSAHRWTPDVPMIIPEVNPEHAEIIAFQKKRLGTKRGFIAVKPNCSI
                     QSYAPLLAAWKEYVPTQVVASTYQAISGAGKTFRDWPEMIGNIIPYIGGEEEKSELEP
                     LRILGKIENGQIVKATAPAITTQCIRVPVLDGHTAAVFINFEKKPTKEQLIEKMIGYK
                     GLPQELDLPSAPKQFIQYMEEENRPQVILDVEYENGMGISIGRLREDTVFDYKFVGLS
                     HNTIRGAAGGAVLCAELLKAKGYIEKK"
BASE COUNT          388 a          225 c          352 g          288 t
ORIGIN      
        1 gtgtggagag aaggcttacg atcggcgctc cgggacaggg ggcaatgaaa gaagtcatcc
       61 ggctttataa ggaattttta cgttgtccgc aagatgggca gcggggaaaa taaaggagaa
      121 aagaggagat ctatatgagt gaaaaattaa aagtgggtat attaggcgga acaggaatgg
      181 taggacaaag atttatttca ctgcttgcag accatccgtg gtttgagatt actgtcattg
      241 cggccagtga aagaagtgcc ggaaaaactt atgaagaggc agtcggacac aggtggaaga
      301 tcgcatgttc catgccggaa tcggtaaaga agatcattgt catgaatgtg aatgaggtgg
      361 aggcggttgc ttctaaagtt gattttgtat tcagtgccgt agatatgaca aaagaagaga
      421 tccgtgcgat agaagaagca tatgcaaaga cagagacacc ggtggtatcc aataacagtg
      481 cacacaggtg gacaccggat gttccgatga tcattccgga agtcaatccg gagcatgcgg
      541 aaattattgc attccagaaa aagaggcttg gtacgaaacg tggatttatc gcggtaaaac
      601 cgaactgctc catacaaagc tatgcgccgc ttcttgctgc gtggaaagag tatgtaccga
      661 cacaggtcgt ggcttcaacg tatcaggcaa tatcaggagc tggaaaaact tttcgggact
      721 ggcccgagat gatcggaaat attattcctt acattggcgg agaagaggaa aagagcgaac
      781 tggaaccgct ccgtattctt ggtaagatcg aaaacggaca gatcgtaaag gcaacggcac
      841 cggctattac aacacaatgt atccgtgtac cggttttgga cggtcatacg gctgctgtgt
      901 ttattaactt tgaaaaaaag ccgacaaagg agcagctgat cgaaaaaatg atcggctata
      961 agggacttcc gcaggaactg gatctgccaa gtgctccaaa acagtttatt cagtacatgg
     1021 aagaggaaaa tcgtccgcag gtaatactgg atgttgagta tgaaaatggt atggggattt
     1081 ctatcggcag actgagagag gatacggtct ttgattacaa atttgtcgga ctttcccata
     1141 atacgatcag aggcgctgca ggcggagcgg ttctttgtgc tgaactgtta aaggcgaaag
     1201 gctatatcga aaagaaataa acagacctgt actgtctcgt aaggtgcggc tgc
//
LOCUS       DAOSYE010000793         1252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102531_length_1252_cov_2.807853, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000793 DAOSYE010000000
VERSION     DAOSYE010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102531_length_1252_cov_2.807853"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..866
                     /locus_tag="PLU43_11595"
     CDS             444..866
                     /locus_tag="PLU43_11595"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO29099.1"
                     /translation="MQNVVLFGFAVIAAAMDLSFRKVKNWLIMAGFLAGMFFQIHGSG
                     IQGLTAGLLGAAVPLICLGVFFVLRLLGAGDLKLLSVIGCFMGPDKILYCIGAAILAG
                     GAIGIVKLLFCKKKGEKTTICFAVPVLISVLLYQGGVY"
     gene            863..>1252
                     /locus_tag="PLU43_11600"
     CDS             863..>1252
                     /locus_tag="PLU43_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29100.1"
                     /translation="MKSELLVICDSQAGYAKKLAGYFRKKACHYEIQVFTDSSVFLAA
                     AEQMTVKALLIGEDIFEILRNEKKTEKLQAEKLLVLTERRDYKQKEKEEAVYKYQSGE
                     AILYCLVNEGLEAAEINKEKGFLNRTEM"
BASE COUNT          384 a          193 c          294 g          381 t
ORIGIN      
        1 cgtatcttct ctgccatcaa agtatacgga aatcaaagct ggcagtccgc gctgcacacg
       61 tatttgatcg tgcgacggta cgaaactgga aaactgttca taaaatatat gatatgatgc
      121 ttgccttata aatttcttat atttgtccac gaaatataca aaaaatggga ttttgtattg
      181 tcataattaa taagaacaga ggtagtaaag aaagtatgga tattcgattt ttgtgagaaa
      241 ttgccaaaga ttgaaaacat gctttttttt tatagacaag cagcagatcg ttctttataa
      301 ttctaattac aaattaattt gcacatatgt tttacatatc aatcttgata ccggcgcaca
      361 gcagccggaa tgcaattgcg tagttttcca aatccctgtg agtatttgat ttatgatcct
      421 ttaaaagaaa agttgagtcc atcatgcaaa atgttgtatt attcgggttt gcggtcatag
      481 ctgcagctat ggacctctct ttccgtaaag taaaaaactg gctgatcatg gcaggctttt
      541 tggccggaat gttttttcag atccatggca gcggcataca aggtctgaca gcgggtcttt
      601 taggagctgc agttccgctt atctgcctgg gggtattttt tgtacttcgg cttttggggg
      661 cgggagatct gaagctgctt agtgtaatag gatgctttat gggtccggac aaaattttat
      721 attgtattgg agcagcgatt ttagcaggcg gtgccattgg tattgtgaaa ctgctgtttt
      781 gtaaaaagaa aggtgaaaaa acaactattt gttttgcggt gcccgtattg atcagtgtcc
      841 ttttatatca gggaggtgtt tattgaaatc agaattattg gtgatctgtg attcacaggc
      901 cggttatgca aaaaagcttg ccggatattt cagaaaaaaa gcatgtcatt atgagattca
      961 ggtatttaca gatagctctg tttttttagc agcggcggaa cagatgacag ttaaagcgct
     1021 gctgatagga gaagatattt ttgaaatcct taggaatgaa aagaagacag aaaaattgca
     1081 ggccgaaaaa ctgcttgttc ttacggaacg gcgggattac aagcaaaaag agaaagaaga
     1141 agcagtatat aaatatcagt ccggggaggc gattctgtat tgtttagtca acgaaggatt
     1201 agaagccgca gaaataaata aggaaaaagg gtttttgaac cggacggaaa tg
//
LOCUS       DAOSYE010000794         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102704_length_1251_cov_2.011706, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000794 DAOSYE010000000
VERSION     DAOSYE010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102704_length_1251_cov_2.011706"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLU43_11605"
     CDS             <1..715
                     /locus_tag="PLU43_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO29101.1"
                     /translation="YSNASERLLENAYYILTPEEHVPIEKIQEFKTFIASLNALPIVL
                     NVDLHDTVTAAVSHLPHIVAVSLVHFLISHDGQEHYMKTLAAGGFKDMTRIASSNVRM
                     WEDICMTNQEKIDRMLTDFIEELSDVQKRIASKNRLEIGSFFQEAKDYRDSMSDHTQG
                     LIQKVFALYCDIPDETGCIAKIAVLLAEDDISIKNIGIIHNREFEEGVLRMEFYNELS
                     SKKALLLLKENGYSVIERN"
     gene            727..>1251
                     /locus_tag="PLU43_11610"
     CDS             727..>1251
                     /locus_tag="PLU43_11610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568772.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOO29102.1"
                     /translation="MIFKKVSSMKGELTIPGDKSISHRAVMLGSLADGTTEIHHFLNG
                     ADCLSTISCFRALGIDIEETDAETVLVHGKGLYGLHSSEMTLNAGNSGTTTRLLSGIL
                     CAQPFSSTLSGDDSVNRRPMGRIIKPLTLMGASIKSRTGNDCAPLLIDGRPLKGIHYE
                     SPVASAQVKSAVLFA"
BASE COUNT          359 a          298 c          283 g          311 t
ORIGIN      
        1 atatagcaat gcatccgaac gtcttttgga aaatgcctat tacatactga cacctgaaga
       61 acatgtccca attgaaaaaa ttcaggaatt caaaacattt attgcctctc tgaatgcgct
      121 gccgatcgtt ctgaatgtag acctgcacga cacggtcaca gctgccgtca gccatctgcc
      181 gcatatcgtt gccgtttctc tcgtacattt tctgatcagt catgacgggc aggaacacta
      241 tatgaaaaca ctggcagccg gcggattcaa ggacatgaca agaatcgcat cttccaatgt
      301 ccggatgtgg gaagatatct gtatgacaaa tcaggaaaag atagaccgga tgctgactga
      361 ttttattgaa gagctttcag atgttcaaaa acggatcgcg tcaaaaaacc ggcttgaaat
      421 cggcagcttt tttcaggagg caaaagacta cagggattcc atgtcggacc atacacaggg
      481 actgattcag aaagtatttg cactgtactg cgatatcccg gacgaaacag gctgcattgc
      541 caaaattgcc gtacttctgg ccgaagatga catcagtata aaaaatatcg gtattattca
      601 taaccgggag ttcgaagaag gtgtcctgcg tatggaattt tacaatgagc tctcttccaa
      661 aaaagcactt ctgcttttaa aagaaaacgg atattccgta atagaaagga attagcagcc
      721 atgtccatga tattcaaaaa agtttcttcc atgaaagggg agctgactat ccccggtgat
      781 aaatctatct cccaccgggc cgttatgctc ggctcactgg cagacggtac gactgaaatc
      841 catcattttc tgaacggtgc cgactgtttg tccacaatca gctgtttcag ggcacttggc
      901 attgatattg aagaaacgga tgctgagact gtacttgtac acggaaaggg actatatggg
      961 cttcattcat cggaaatgac gcttaacgcc ggaaacagcg gtacaacgac aagactgctg
     1021 tccggtattc tctgcgcaca gcccttctct tcaacgcttt caggcgacga ctccgtcaac
     1081 cgccggccga tgggacgtat cataaaaccg ctgactctta tgggggcctc cataaaaagc
     1141 agaacaggca atgactgtgc ccctctcctc attgatggac gtccgctgaa aggaattcat
     1201 tacgaaagcc ctgttgcatc cgcgcaggtc aaatcggccg ttctttttgc c
//
LOCUS       DAOSYE010000795         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102775_length_1250_cov_3.041841, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000795 DAOSYE010000000
VERSION     DAOSYE010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102775_length_1250_cov_3.041841"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLU43_11615"
     CDS             complement(<1..516)
                     /locus_tag="PLU43_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO29103.1"
                     /translation="MGKTVFIAEKPSVAREFAKALKINAKKHDGYLESENTIVTWCVG
                     HLVTMSYPDVYDIKYKKWSFTTLPFLPDTYLYEVIPSAAKQFAIVSSLLTREDVETIY
                     VCTDSGREGEYIYRLVEQQAHVTGKIRKRVWIDSQTEEEIRRGIKEAKDLSAYDNLSE
                     AAYLRAKEDYLM"
     gene            complement(557..>1250)
                     /locus_tag="PLU43_11620"
     CDS             complement(557..>1250)
                     /locus_tag="PLU43_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HOO29104.1"
                     /translation="GMHLLPEELREPSVYNTILLTLASGKQKLNELYKHTGFNRAKIS
                     VYLKNLIELELVEKVDSYDTAGKENTQKGLYRISNQFVHFWYRYVFPDLSMLQIMSPK
                     KYFDKYIEPSFKSYTSECFTKVCKEYLCLMNKMNQLHFKFVKMGSWVGKVGTIDIVAQ
                     DEDGRTLIGCCNWEKSLMTYEDYEWLLFCAEQAKLKADDIYLFTAGGFDEQLKLEANV
                     KKNMYLIDSTRL"
BASE COUNT          338 a          296 c          206 g          410 t
ORIGIN      
        1 catcagataa tcttctttgg cacgcaaata cgcagcttcc gaaaggttat cataagcgga
       61 cagatctttt gcctctttaa ttcctcttcg aatttcctct tcagtttgtg aatcgatcca
      121 gactcgtttt cttatttttc ctgtgacatg tgcctgctgt tccacaagac gatagatgta
      181 ttctccttca cgtcccgaat ccgtacagac atagatcgtt tccacgtctt cccttgtcag
      241 cagactgctg acgatcgcaa actgctttgc cgcggaagga atgacttcgt ataaataagt
      301 atccggtaaa aaaggaagtg ttgtaaaact ccatttttta tactttatgt catagacatc
      361 cggataactc attgtaacaa gatgtccgac acaccatgta acgatcgtat tttccgattc
      421 caggtaaccg tcatgctttt tcgcatttat tttcagcgct ttggcgaatt cccttgcaac
      481 acttggtttc tccgcaataa aaactgtttt tcccataaat atttcctgta aacttcttaa
      541 ttgatttcca atgccgttac aaccgcgtac tgtcgatcag atacatgttt ttctttacat
      601 ttgcttccag cttcagctgc tcatcaaatc cgcctgcggt aaataaataa atatcatcag
      661 ctttcagctt tgcctgttcc gcacagaata aaagccattc ataatcctcg tacgtcatca
      721 gactcttttc ccagttacag cagccgatca gtgtccgtcc atcctcgtcc tgagctacaa
      781 tatcgatcgt tccgactttt cctacccagc ttcccatctt aacaaattta aaatgaagct
      841 gattcatttt attcatcaga cataaatatt ccttgcatac ctttgtaaaa cattcggatg
      901 tatatgattt aaatgacggc tcgatatatt tatcaaaata tttcttcggg ctcatgattt
      961 gcagcatgga aagatccggg aaaacataac gataccaaaa gtgtacaaac tgatttgaaa
     1021 tacgatagag tcccttttgt gtattttcct ttcctgcggt atcataagaa tcaacttttt
     1081 caacaagctc cagctcgatc agatttttta gatacacgct gatcttggcc cggttgaatc
     1141 cggtatgttt atacagttca ttaagcttct gcttaccgga agccaatgtc agcaatattg
     1201 tattatagac ggaaggttct ctcaattcct ccggaagaag atgcatgcct
//
LOCUS       DAOSYE010000796         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102783_length_1250_cov_2.825941, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000796 DAOSYE010000000
VERSION     DAOSYE010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102783_length_1250_cov_2.825941"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="alr"
                     /locus_tag="PLU43_11625"
     CDS             <1..552
                     /gene="alr"
                     /locus_tag="PLU43_11625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO29105.1"
                     /translation="CAESGVHPLYLHCSNSAGILEIPWANMDLVRAGITMYGLWPSDE
                     VSRDVIDLIPAMTIKSTIVYIKTLPKGVQISYGGTYTTEKTMRIATIPVGYADGYPRG
                     ASNKGDVLIRGMRAPIVGRVCMDQFMVDVTDIPGVQEYDEVVLLGSFLGEKITAEEIG
                     NISGRFNYELVCDISGRVPRIYK"
     gene            657..>1250
                     /locus_tag="PLU43_11630"
     CDS             657..>1250
                     /locus_tag="PLU43_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO29106.1"
                     /translation="MSGHSKFANIKHKKEKNDAAKGKIFTIIGREIAVAVKEGGPDPV
                     NNFKLAQVIVKAKANNMPNDTIERGIKKAAGDAGSVNYEYVTYEGYGPNGIAIIVDAL
                     TDNKNRTAANVRSAFTKGSGNVGTPGCVSFMFDKKGQIIVDKEICDLDADELMMAALD
                     AGAEDFSEEEDSYEILTAPDDFSAVCDALEQAKIPAVS"
BASE COUNT          410 a          205 c          330 g          305 t
ORIGIN      
        1 tgtgcggagt caggtgttca tccattatat ttgcattgtt ccaacagcgc aggtatattg
       61 gaaattccgt gggcaaatat ggaccttgtg cgggcgggta tcaccatgta tgggttatgg
      121 ccgtccgatg aagtatccag agatgtgata gatcttattc cggccatgac gataaaaagt
      181 accattgttt atatcaaaac actgcccaaa ggagtgcaga tcagttatgg gggcacttat
      241 acgactgaaa aaaccatgcg tattgcaacg attcctgttg gctatgcaga cggatatcca
      301 agaggagcat ccaataaagg agatgtattg atcagaggta tgcgcgcgcc gatcgtagga
      361 agggtctgta tggatcagtt catggtggat gtgacggata ttccgggagt acaggaatat
      421 gatgaagtcg ttcttcttgg cagcttttta ggagaaaaaa tcacagcaga agaaatcgga
      481 aatatatcag gaagatttaa ttatgaactt gtctgtgata tttcgggcag ggttccgcga
      541 atatataaat agcatgtttg gaatttacat taattataaa aaagttgaag aaacaaaaaa
      601 aaaatgctac aataaaaacc agtatggata aaacaagcag agaatgaggt gtagaaatgt
      661 caggacattc taaatttgct aacattaagc ataaaaaaga aaagaatgat gcagcaaagg
      721 gaaagatatt tacgatcatc ggaagagaaa ttgccgtagc cgtaaaagaa ggcggaccgg
      781 accccgtgaa caatttcaaa ctggcgcagg taatcgtaaa agcgaaggca aataatatgc
      841 cgaacgatac gattgaaaga gggatcaaaa aagcagccgg agatgccggc agtgtaaatt
      901 atgaatatgt gacatatgaa ggttacgggc cgaacgggat tgcaatcatt gttgatgccc
      961 ttactgataa taagaatcgc acggccgcca atgtaagaag cgcgtttacg aaaggcagcg
     1021 ggaatgtagg aactcccggg tgcgtttcct ttatgtttga taaaaaggga cagatcattg
     1081 ttgacaagga aatatgtgat ctggatgcag acgaattgat gatggcggct ctggatgcag
     1141 gtgccgaaga tttttcggaa gaagaggaca gttatgagat cctgactgcg ccggatgatt
     1201 tttcagcagt ctgtgatgct ttggaacagg ccaaaatccc ggcggtttcg
//
LOCUS       DAOSYE010000797         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_102792_length_1250_cov_2.666109, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000797 DAOSYE010000000
VERSION     DAOSYE010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102792_length_1250_cov_2.666109"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLU43_11635"
     CDS             <1..451
                     /locus_tag="PLU43_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29107.1"
                     /translation="FSSSWLSNPIKAFWSLVIVTVWQLSGYLMLIYIAGLMGVPEDLK
                     EAAKIDGCDEKKTMRHITMPLMMGTFTICFFLAITRSFVIYDVNISLTEGGPYGSTRL
                     AAMYVYNMAFSSKKYGLGQAEALILFVVIAVIAVSQALLGKKKEVEA"
     gene            451..>1250
                     /locus_tag="PLU43_11640"
     CDS             451..>1250
                     /locus_tag="PLU43_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29108.1"
                     /translation="MGTSKDINKESQIKRTIGGVVKMIVLLIFFVIYMFPFIMVVINS
                     FKSKRDIIKEPIALIGSQGASFKNYVAAFEKMNFPRAFMNSFLITVGSVLLIVITSAM
                     CAYLFVRMEYKINKIIFTLMIASMVIPFQVIMIPLVLIYGGQLHVLNHRITLILMHTG
                     FSTAMAVFMYHGFIKSSIPLSLEEAARLDGCSRHQTFFRIVLPLLKPTTATLIILYSM
                     GIWNDFLLPSLVLTEKELYTLPIATQMFYGTYSSDLGLIMASLIMVVIP"
BASE COUNT          341 a          233 c          281 g          395 t
ORIGIN      
        1 tttttcttca tcatggcttt ccaatccgat caaagcattc tggtcattgg tcatcgtaac
       61 agtatggcag ctgtcaggtt acctgatgct gatttatatt gcaggattga tgggggttcc
      121 tgaagatctg aaggaagcag ccaaaattga cggctgtgat gaaaaaaaga caatgcggca
      181 tattacgatg cctcttatga tgggaacgtt tacgatctgt ttcttccttg ccatcacaag
      241 aagctttgta atatatgatg ttaatatttc cctgacagaa ggcggacctt atggttctac
      301 aaggctggca gccatgtatg tttataatat ggcattctcc tctaagaaat acgggctggg
      361 tcaggcagaa gcacttattc tgtttgttgt gattgcggtg atcgccgtat cgcaggcatt
      421 gcttgggaag aaaaaggagg tggaagcata atgggtacca gtaaggatat taacaaagaa
      481 agccagataa aaagaacaat tggcggcgtt gtaaaaatga tcgttctttt gattttcttc
      541 gtgatctata tgtttccgtt tattatggta gtcatcaact cctttaaatc taagagagat
      601 attattaagg agccgatcgc attgatcgga agccaggggg cttcctttaa aaattacgtg
      661 gcagcttttg aaaaaatgaa tttcccaaga gcttttatga attcattcct tataacggta
      721 ggcagtgtac tgctgatcgt aattacttca gcaatgtgcg catatttatt tgtacggatg
      781 gaatataaga tcaataaaat aatttttaca ttgatgatcg catccatggt aattcctttt
      841 caggtaatca tgattccgtt ggttttgatc tatggcggac aattgcatgt gctgaaccac
      901 aggatcacac tgatcttgat gcatacaggg ttttcaactg ccatggccgt gtttatgtac
      961 catggattta taaaaagcag tattccgctg tcgcttgaag aagcggccag actggacgga
     1021 tgctccaggc atcagacgtt tttcaggatc gtgcttccgc ttttgaagcc tacgacagca
     1081 acactgatca tcttatattc catgggaatc tggaatgact tccttttacc gtcgcttgtt
     1141 ttaacggaaa aggaattata tacacttccg attgcaacac agatgtttta cgggacgtat
     1201 tcttctgacc ttggtctgat catggcttcc ctgatcatgg ttgtaattcc
//
LOCUS       DAOSYE010000798         1246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_103338_length_1246_cov_3.519731, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000798 DAOSYE010000000
VERSION     DAOSYE010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103338_length_1246_cov_3.519731"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLU43_11645"
     CDS             <1..850
                     /locus_tag="PLU43_11645"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HOO29109.1"
                     /translation="NYAALMIDLKRSRSYSVEDRTSIQEYMLTVIQSLNKLFSKTLAM
                     DVEFSAGDEVQGLFTFPEAAYLYFRLFCMLVFPVESRAGIGVGEWNVKIKNASTAAQD
                     GPAYHNARYAIEQVKAVPGYSILFYTESETDLFLNAAANMFFALTGSHSEYQNELMLL
                     LELLYPINCYHAIDCEKIKLLSTLITEKNKLHYYMEYKKSRSVKKYPFDKAEYMEFES
                     IPINAAEDDSTFYVSGGMKRGMITKLSEALNISRQTIEKTVKTANIYEARNSVIVTLK
                     FMDQYL"
     gene            860..1243
                     /locus_tag="PLU43_11650"
     CDS             860..1243
                     /locus_tag="PLU43_11650"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOO29110.1"
                     /translation="MLLYILLAVHFLADFTFQTRKVAEKKVHKNSYLIIHALIYAGVF
                     LPVLFLAIKFEKAVLSYMIIIGSHFLIDWFRKAVEQKYTSFRFFSFLADQVLHILILV
                     IVYYAFDLSVSQQIIIPTYCNFTAD"
BASE COUNT          391 a          209 c          260 g          386 t
ORIGIN      
        1 aaattacgct gcattaatga tagatttgaa aagatccagg tcatattcgg tcgaagacag
       61 aacttccata caggagtata tgcttactgt tattcaaagt ctgaataaac tattttcaaa
      121 gacattggct atggatgtag aatttagtgc gggagatgag gtgcagggat tatttacgtt
      181 tccggaagcg gcgtatctct atttcagact gttttgcatg cttgtttttc cggttgaaag
      241 cagggccggc attggtgtcg gagaatggaa cgtaaagatc aaaaatgcaa gtaccgcagc
      301 gcaggacgga ccggcatatc acaatgcacg ttacgcgatc gagcaggtaa aggctgtgcc
      361 tggatattcc atactttttt acacagaaag cgagacggat ctgtttttaa atgctgctgc
      421 caatatgttt tttgcactga caggcagcca tagcgaatat cagaatgaac tgatgctgct
      481 tttggagctg ctttatccga tcaactgtta tcatgcgatc gactgcgaga aaataaagct
      541 gctttccaca ctcataacag aaaaaaataa acttcattat tatatggaat ataagaaaag
      601 cagatcggta aaaaagtatc cttttgacaa agccgaatat atggaatttg aatccattcc
      661 gataaatgcg gcagaggatg acagcacttt ttatgtttcc ggcggaatga aaagaggaat
      721 gatcacaaaa ttatccgaag ctttaaacat aagcaggcaa acgattgaaa agactgttaa
      781 gacagctaat atttatgaag ccagaaattc agtgatcgtt acattaaaat ttatggatca
      841 gtatttataa gggggaaata tgctgctgta tattttgctg gcggttcact ttcttgctga
      901 ttttactttt cagacaagaa aagtggcaga aaagaaagtt cataaaaatt catatcttat
      961 tattcacgca ttgatttacg ccggtgtatt tttaccagtt ctgttcctgg cgataaaatt
     1021 cgaaaaggct gttttgtcat atatgataat cataggctcc cattttttaa ttgactggtt
     1081 cagaaaggcg gtggaacaga aatatacatc gtttagattt ttttcttttc ttgcagatca
     1141 agtgctgcat attctgattc ttgttattgt atattatgcg tttgacttaa gtgtcagcca
     1201 gcaaattata attccaacct actgtaactt taccgctgat taaatt
//
LOCUS       DAOSYE010000799         1244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_103609_length_1244_cov_4.806560, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000799 DAOSYE010000000
VERSION     DAOSYE010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103609_length_1244_cov_4.806560"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1244)
                     /gene="lysS"
                     /locus_tag="PLU43_11655"
                     /pseudo
     CDS             complement(38..1244)
                     /gene="lysS"
                     /locus_tag="PLU43_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
BASE COUNT          273 a          341 c          282 g          348 t
ORIGIN      
        1 ggatttccat agtcttcacc gaatccatac actaatttag actcttcatc gtcgggaaca
       61 gcagtacatc cctgatcgcc ggcgcattcg tcagcaacat gacaaggcgg tcgatgccat
      121 agccgatacc gcctgtaggc ggcatgccga tctcaagggc attcatgaag tcttcgtctg
      181 ttgtgttggc ttcttcgtca ccctgtgcaa gaagtgcttc ctgtgctttg aaacgctcgc
      241 gctggtcgat cgggtcgttc agctctgaat aggcattgca catttcgcgg caggtgatga
      301 acagctcaaa acgctccaca taatccggtt tgtccggctt tctctttgtc agcggtgaga
      361 tctcgaccgg atggtcggtc acaaacgttg gctggatcag atgctcctct acgaattcct
      421 caaagaaaag acttaagatg tcgccctttt catggcgggg ttcgtagtgt acctcttttt
      481 catcggcgat cttctttgct tccgccgtat ccttgatctc atcaaaatcg attcccgtat
      541 attccttcac agcatcgatc atcgtaattc ttttaaacgg actgccaaga tcgatctcca
      601 catcaccgta tgggatcgtc gtcgtaccgc atacttcctg cgccacgtgg cggaacatat
      661 tttccgtcag atccatcatg ccgttataat ccgtgtaggc ctgatagagc tccatcaagg
      721 tgaattccgg gttgtgtctt gtatcgagtc cctcattgcg gaatacacgg ccgatctcgt
      781 atacacgttc cataccgccc acaatcaatc tttttaaata caactccagg gaaatacgca
      841 gcttgatatc ctcatcaagt gcattataat gcgtttcgaa cggtctggct gcggcgccgc
      901 cggcatttgg tacaagcatc ggcgtctcca cttccaaaaa tccctgtccg tccagatagc
      961 ggcggatcga gctgatgatc tgggaacgtt ttacgaacgt ttcctttacg tcctcattca
     1021 taatcagatc cacatatctc tgacggtatc tgatgtccgt attggtcagg ccatggaatt
     1081 tctcaggcag gatctgcaga cttttggaca gcagcacgac tttggatgca tggatggaga
     1141 tctcgccctt ctgggtctta aataccgtgc cctccacacc gatcagatcg ccgacgtcga
     1201 attttttaaa tgcggcatac ggttcctcac cgatcgcgtc tctg
//
LOCUS       DAOSYE010000800         1242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_103930_length_1242_cov_3.130581, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000800 DAOSYE010000000
VERSION     DAOSYE010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103930_length_1242_cov_3.130581"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLU43_11660"
     CDS             <1..375
                     /locus_tag="PLU43_11660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO29111.1"
                     /translation="PLLLINGLIWGLWHAPLIVVGHNYGTEYDGYPYLGIAAMCVFCI
                     VLGIFFSYVTMKTKSCFPSIIAHGAVNGFASAGVYFTDNGGNAFVGPAITGIVGGLPF
                     IITAVILAVLMKRDHTLETYNA"
     gene            503..>1242
                     /locus_tag="PLU43_11665"
     CDS             503..>1242
                     /locus_tag="PLU43_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO29112.1"
                     /translation="MQRPGGTGSIDRGARVRLIKKVIIGLLAAVILLPTLLCVVLFFK
                     LNKIEQECRDLSERMLVMEKSSDDMYTAVSMERKNAEDAVSFNEIENKESDTPEDVQA
                     LPDAAQISKEESVSEDETAHTDWPKKVYLTFDDGPSSNTAAILDILNSYGVKGNFFVV
                     ATDKEELRAMYKRIVDEGHVIGMHSYSHKYDEIYASKEAFINDLSKIQSLVYEQTGYM
                     PDIYRFPGGSSNLVSKISMSVFINVLNE"
BASE COUNT          361 a          200 c          317 g          364 t
ORIGIN      
        1 ccgcttctgc ttattaatgg tttgatctgg gggctttggc atgcaccttt gatcgtagtc
       61 ggccataatt atggaacgga gtatgacgga tacccttatt tgggaattgc ggcaatgtgt
      121 gtgttttgta ttgttcttgg aatttttttc tcttatgtca cgatgaaaac gaaaagctgt
      181 tttccttcta ttattgcaca tggggccgtt aatggatttg catcagcagg tgtctatttt
      241 acagataacg ggggaaatgc atttgtcggt ccggcaatta cgggtattgt cggcggactt
      301 ccttttatta taaccgctgt tattcttgct gttttaatga aaagggatca tacgctggaa
      361 acttataatg catgatgcaa atatcaaaat aatgagcagg ataatttgca aaacttacgg
      421 atatatttaa aaaagtgttt tctaaagatt aacaaatgtg gtacaatgaa atgcggatga
      481 taattgggaa ggacagatag tcatgcaaag acctggcggt acggggagca tagacagagg
      541 tgccagggtg cgcctgatca aaaaggtaat tattgggctg ctggctgcgg ttattttact
      601 tccgacattg ctttgtgtgg ttttattttt taaattaaac aagatcgagc aggagtgcag
      661 agatctttcg gagcgtatgc ttgttatgga gaagagctca gatgatatgt atacggcagt
      721 ttccatggaa agaaagaatg cggaggatgc cgtgtcgttt aatgagatcg aaaacaaaga
      781 aagcgatacg ccagaagacg tacaggcact gccagatgcg gcgcagatat cgaaagaaga
      841 gagtgtttcc gaagacgaaa cggctcatac ggactggccg aaaaaggttt atctgacatt
      901 tgatgacggt ccaagcagca atacggcggc cattttggat atactaaaca gttatggggt
      961 aaaaggtaat ttttttgttg tggcaacaga taaggaagaa ctgcgcgcaa tgtataaacg
     1021 gatcgtggat gaggggcatg tgatcggcat gcattcgtac tcacataaat acgatgagat
     1081 ctatgcatca aaagaagcct ttatcaatga tctttccaaa atacagtcgc tcgtttatga
     1141 gcagaccggt tatatgccgg atatttatcg ttttccgggc ggcagcagta atctggtatc
     1201 caaaatcagt atgtcggtat ttattaatgt tttgaatgaa ag
//
LOCUS       DAOSYE010000801         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_104667_length_1237_cov_3.318951, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000801 DAOSYE010000000
VERSION     DAOSYE010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104667_length_1237_cov_3.318951"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLU43_11670"
     CDS             complement(<1..929)
                     /locus_tag="PLU43_11670"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOO29113.1"
                     /translation="MGFEFIKKLPTPAQIREELPLTKDLIQLKKERDLQISDIIMGNT
                     DKFLVIVGPCSADNEDSVCDYISRLVKIQEDTKEKLLIVPRIYTNKPRTTGSGYKGIV
                     HQPDPEKKPDFLQGLLAMRKMHIRAMKETGLTAADEMLYPDNWGYVSDILSYVAIGAR
                     SVEDQQHRITVSGFDVASGMKNPTSGDFSVMLNSVMAAQNPHSFIYRGWEVNTSGNPL
                     AHTILRGAVDRHGSSIPNYHYEDLNLLLSMFQERDLKYPATIIDANHSNSNKKYAEQI
                     RIVKEVLHSRRLSPDIRQLVKGVMIESYIEAGC"
     gene            1099..>1237
                     /locus_tag="PLU43_11675"
     CDS             1099..>1237
                     /locus_tag="PLU43_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO29114.1"
                     /translation="MKKIIYLDNAATTKTAPEVVEAMLPYFTELYGNPSSVYEPAALS
                     KK"
BASE COUNT          331 a          252 c          278 g          376 t
ORIGIN      
        1 tgacagccgg cttcaatata gctttcgatc ataacgcctt ttacaagctg acgaatgtcc
       61 ggacttaggc ggcggctgtg caggacttct tttacgatcc tgatctgctc agcatatttt
      121 ttattggaat tggaatggtt tgcatcaata atagtagccg gatatttcag atcccgttcc
      181 tggaacatgg agagcagaag attcagatct tcataatgat aatttggaat gctggaacca
      241 tgtctgtcga cagcaccgcg caggatcgta tgtgcgagtg gattgccgga cgtatttact
      301 tcccatccgc ggtaaataaa gctgtgtgga ttctgggctg ccatgacgga attcagcata
      361 acggaaaaat caccgcttgt tgggtttttc ataccgctgg ccacatcaaa gccactgacg
      421 gtgatcctgt gctgctggtc ttcaacagag cgtgcaccga tcgccacata ggataaaata
      481 tcggaaacat acccccagtt atcagggtag agcatttcat cggctgcagt cagtccggtt
      541 tccttcattg cacggatgtg cattttacgc atggcaagca gtccctgaag aaagtccggt
      601 tttttctcag gatccggctg atgtacgatc cctttatatc cggagccggt cgtccggggt
      661 ttatttgtgt agattcttgg gacaatgaga agtttttctt tcgtatcttc ctgtattttt
      721 acaagacgcg aaatatagtc acagacagaa tcctcgttat cggccgaaca gggaccgaca
      781 atcacgagaa atttatctgt gtttcccata ataatgtcgg aaatctgcag gtctctctct
      841 ttttttaatt gaatcagatc tttcgttaac ggaagttcct cacggatctg tgccggtgtc
      901 ggaagctttt taataaattc gaatcccatg tttgttcctc attctctata taatgttcaa
      961 cgcttatcat acatgattaa ctgttttttt tcaactttaa attattttgc tttattgaca
     1021 gagagaatag atggtgttat gatgcatata gatgaattcg aagtggatta ctaggaaatg
     1081 aagattggag tcttgtatat gaaaaaaata atttatttag ataatgcggc aacaacaaag
     1141 accgcaccgg aagtcgtaga agcgatgctt ccatatttta ctgaattata cggaaatccg
     1201 agcagtgttt acgagcctgc ggcactgagt aaaaaag
//
LOCUS       DAOSYE010000802         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_105789_length_1229_cov_4.218058, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000802 DAOSYE010000000
VERSION     DAOSYE010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105789_length_1229_cov_4.218058"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLU43_11680"
     CDS             complement(<1..413)
                     /locus_tag="PLU43_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOO29115.1"
                     /translation="MEVKKQHISVPLKIALVYFSATGNTKKIADTIKNYLAARRVFVT
                     SIDITSYESRKKQLAFDEYDAVIFGFPIYSLRAPRICRQWLETLDGTGKKCAVFFTFG
                     GFGKDPAHYYMKELLEKRSFTLVSTAEFLAVHTFNR"
     gene            759..>1229
                     /locus_tag="PLU43_11685"
     CDS             759..>1229
                     /locus_tag="PLU43_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO29116.1"
                     /translation="MSLKLSEKAQAVKPSSTLAITAKAKAMRAEGIDVVGFGAGEPDF
                     NTPENVCNAAIDAIHAGFTKYTPASGTEELKKAISKKFREFNHLDYAVNQIVISNGGK
                     HALSNVFDAILNAGDEVIIPAPYWLSYPEMVKLSDGVPVFVYGLKEQGYKVSAAQ"
BASE COUNT          371 a          211 c          280 g          367 t
ORIGIN      
        1 cggttgaatg tatgtactgc taaaaattcc gcggttgaca cgagggtaaa gctgcgtttt
       61 tcaagtaact ctttcatata ataatgggcg ggatcctttc caaatccgcc aaaggtgaag
      121 aataccgcac atttttttcc ggtaccgtca agcgtttcaa gccactgtct gcatatgcgc
      181 ggcgctctta aagaataaat gggaaatccg aatataacgg cgtcatattc atcaaaagcc
      241 agttgttttt tcctgctttc ataagatgtt atatcgatgc ttgtaacaaa aacacggcgg
      301 gctgctaaat aattttttat tgtgtctgcg attttttttg tgttgccggt tgcagaaaag
      361 tatacgagtg cgattttaag ggggacagaa atgtgctgct tttttacttc caaaatatga
      421 tcaatccttc ctttttgttt tagtaactta aatataattc ataaagctcg tgtttgtcaa
      481 taacagcatt tttaaatgtc atcatagata tctttcataa taattagtac tattataaca
      541 gataaagtcc gccatagaaa gtattaggtt tacgtgtccg cgcctggcga acaaatgtaa
      601 atttttttga tttttcactt gtatattgaa aatgcgtgta ttatactcta ctagtatgct
      661 gaaaaagtca gtgcggtgat gcttgagcag aactgccgct gtgtttagcg gaaagctgac
      721 ttatgaggag aatgaaaaag aaaagggtgg aaaatataat gtcattaaaa ttatctgaaa
      781 aagcacaggc tgtcaagccg tcttcgacgc tggctattac tgccaaagca aaggcaatgc
      841 gtgcggaagg aattgatgtc gtaggatttg gagcaggaga acccgatttt aatacacctg
      901 agaatgtatg taatgcggca atcgatgcga tccatgcagg ctttacaaaa tatacgccgg
      961 catcaggaac agaggaatta aaaaaagcga tcagcaaaaa attcagggaa tttaatcatc
     1021 ttgattatgc ggtaaatcag atcgttatca gcaacggggg aaaacatgcg ctttccaatg
     1081 tgtttgatgc gattttaaat gcaggggatg aagtgatcat accggcgccg tactggctga
     1141 gttatccgga gatggtcaag ctttccgacg gtgtgccggt atttgtttat ggtttgaaag
     1201 aacagggcta taaagtgagt gctgcccag
//
LOCUS       DAOSYE010000803         1226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_106284_length_1226_cov_2.481640, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000803 DAOSYE010000000
VERSION     DAOSYE010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106284_length_1226_cov_2.481640"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1226)
                     /locus_tag="PLU43_11690"
     CDS             complement(<1..>1226)
                     /locus_tag="PLU43_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29117.1"
                     /translation="QTLFVFHKAKKFNEQLDVYRTLLSSCQMLYDTICEENEKYIALC
                     SQYGTNVQVLPGIEQLGGFSKDGVMDDWLSEYYTVVSSFPPSKWKAFYENNTAACTGF
                     ILKADEDMQLLLSDCEKLSRYLDMLYDLYLSDFNIDLFTFYLALFKETIQKELPPEPI
                     SNAIENLIQIIEQQTKLDPALRRNRFAEYRSLIPSGSKKTAGSFPHNIDAGILEKIRH
                     SLVNSLDTITNYAGLDDAEKKRFQSLVTQYTALPDRSSSEAFPRSLRKELTALYYTVY
                     KKAFFKSISDTELPAILKMFFYFGYTDEALAGEDNAVYLYYLSMKLTPDENGRVFTFY
                     DWLLQLYHGKKETCVNELNIDYPAYLHKSRVENKITEAEETLALQDGEGRVNYELDNM
                     FHSTNKIVSGHIISFCP"
BASE COUNT          337 a          240 c          287 g          362 t
ORIGIN      
        1 gggcagaaag aaataatgtg tccggatacg attttgtttg tggaatgaaa catattgtca
       61 agctcgtaat ttacacgtcc ttcaccgtcc tgcagtgcaa gtgtctcttc tgcttcggta
      121 attttatttt cgactctgct tttatgcaga taagcaggat aatcgatatt cagttcattg
      181 acacaggttt cttttttccc atgatataat tgcaggagcc agtcataaaa cgtaaaaaca
      241 cggccgtttt cgtccggtgt aagtttcatg gaaagataat acaaatagac ggcattgtct
      301 tctccggcaa gtgcttcgtc cgtataacca aaatagaaga acattttcag gatggccggc
      361 aattctgtgt cactgatgct tttaaaaaag gcttttttgt agaccgtata atacagggcc
      421 gtcagttctt ttctaagact gcggggaaaa gcttcggaag agctgcggtc aggaagtgcg
      481 gtgtactgtg tgaccagaga ctgaaaacgt tttttttcag catcgtcaag accggcatag
      541 tttgtgatcg tatccaggga gttgacaagg gagtgcctga ttttttctaa aataccggcg
      601 tcgatattat gcggaaagct gccggctgtt tttttggagc cggacgggat cagactgcgg
      661 tactcggcaa acctgtttct gcgcagagca ggatcgagtt ttgtctgctg ttcgatgatc
      721 tgtatcagat tttcaattgc attggatatt ggttcaggag gcagctcttt ttggatcgtc
      781 tctttgaaaa gggccagata aaacgtaaac agatcaatat tgaaatcgct taaataaaga
      841 tcatagagca tatccaggta acgggaaagc ttttcgcagt ccgataaaag cagctgcata
      901 tcctcatcag ctttcagaat aaagcctgtg caggcagccg tattgttttc ataaaatgct
      961 ttccattttg aaggcggaaa ggaacttacg acagtataat attcagacag ccagtcatcc
     1021 ataacgccgt cttttgaaaa tccgccgagc tgttcaatgc cggggagcac ctggacgttt
     1081 gtgccgtatt ggctacaaag agcaatatat ttttcatttt cctcacagat cgtatcataa
     1141 agcatctgac aggaggagag aagtgtccgg tagacatcca gctgttcatt gaattttttt
     1201 gccttgtgaa aaacaaacag agtctg
//
LOCUS       DAOSYE010000804         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_106550_length_1224_cov_3.340462, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000804 DAOSYE010000000
VERSION     DAOSYE010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106550_length_1224_cov_3.340462"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLU43_11695"
     CDS             complement(<1..697)
                     /locus_tag="PLU43_11695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO29118.1"
                     /translation="MWIADQWKDYEVLDTSIGEKLERWGDYLLIRPDPQIIWNTKKTN
                     PNWKKINGHYHRNTKGGGEWEFFDLPKQWTIQYDSLVFHLKPFRFKHTGLFPEQAANW
                     DWFSSLIQNAGRPVKVLNLFAYTGGATLAAAKAGASVTHVDASKGMVSWAKENAKASG
                     LENAPVRWLVDDCVKFVEREIRRGNKYDAVIMDPPSYGRGPDGEIWKIEDSVFSLIKL
                     CTKLLSDNPLFFLV"
     gene            839..>1224
                     /locus_tag="PLU43_11700"
     CDS             839..>1224
                     /locus_tag="PLU43_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO29119.1"
                     /translation="MGKRAATASILLAGILWGTMGLFVRGLSVDGFTSMEIVAIRGLL
                     SLLVLAAVIFFYNKKLFKIRIRDCWCFFGTGILSLTFFNYCYFTTIMMTSMSVAAILL
                     YTAPMIVVLLSALLFKEKINARKIFA"
BASE COUNT          309 a          251 c          285 g          379 t
ORIGIN      
        1 tgacaagaaa gaacagcggg ttgtctgaaa gcagctttgt acaaagctta ataagtgaaa
       61 aaacggagtc ttcgatcttc cagatctcac cgtcaggacc gcgtccataa gacggcgggt
      121 ccataataac tgcgtcgtat ttatttcctc tgcgaatttc tctttccaca aatttaacgc
      181 agtcgtcaac aagccaccgg acaggagcgt tttccagtcc actggctttg gcgttttctt
      241 ttgcccatga aaccattcct ttagaagcat ccacatgtgt tacggatgcg ccggcttttg
      301 cggcagcaag cgtggcaccg ccggtgtatg cgaacagatt cagtaccttg accggtctgc
      361 cggcgttttg gatcagggaa gaaaaccagt cccagtttgc ggcctgttca ggaaagaggc
      421 cggtgtgttt gaaacgaaac ggttttaaat gaaaaacaag ggaatcatat tggatcgtcc
      481 attgttttgg gaggtcgaag aattcccatt caccgccgcc ttttgtgttt ctgtgataat
      541 ggccgtttat ctttttccag ttcggatttg ttttttttgt attccagatg atctgcggat
      601 caggacggat caggagatag tctccccatc gttccagctt ttctccgata cttgtatcaa
      661 gaacctcata gtccttccat tggtcagcga tccacatgct gtctgcctcc tgccatatat
      721 acatattttc ttacttaata tacactaata aaagaaagta cgcaattgct attgacgcgt
      781 tgtatttttg tatactataa ttaccgctgt gagtaatatc ggatagagag gctgaaaaat
      841 gggtaaaagg gcagcaacgg caagtatttt actggcagga attttatggg gaacaatggg
      901 gcttttcgta cgcggattat cggtggacgg ctttacttca atggagatcg tggcaattcg
      961 cggactgctg tcattgcttg tattggctgc cgtcatattt ttttataata agaaactgtt
     1021 taaaatcagg atcagagact gctggtgttt tttcggaaca ggaatactga gcctgacttt
     1081 ttttaattat tgttatttta cgacgattat gatgacgtcc atgtcggtcg cggcaattct
     1141 tttatatacg gctcctatga tcgtggttct gctgtcggcc ttgttattta aggaaaaaat
     1201 caatgcgaga aagatatttg cgat
//
LOCUS       DAOSYE010000805         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_106851_length_1222_cov_2.779777, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000805 DAOSYE010000000
VERSION     DAOSYE010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106851_length_1222_cov_2.779777"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /locus_tag="PLU43_11705"
     CDS             <1..>1222
                     /locus_tag="PLU43_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29120.1"
                     /translation="TGGIAGQSIGVIRYSTNYGVVGYEHVGYNVGGIAGRQSGYIYEC
                     NNEGIIYGRKDVGGITGQAEPYIVVDFSKDVVYQLSENINKLHDLIDQTLQDAGSESD
                     TISNRLSVLQSFADKALDDTSYLSDKTVEWTDGVIAAANEAVGRAEYIMDEASKDDGL
                     LDQSEDAAENVKSAAGKLSEVVDDLDIYQYMSDAEKEQYDRDKSSLESATSEHAAYAE
                     DAYNAYEDYYLDEIRSSEDKYKDPSLSVNEIDLRPVVSGNTVTDWTWNSSNTYQNYMD
                     VEDWVHHTASDGDKTFPDPDTGEQNTLDNELLADTQSAMESNAATVESDAASYADSQY
                     LTSHGSVYTTDIESYVDGMAGIVLAHTGEMSDSAKEDAGSAASYAKSASENLKSASGE
                     AKDIVSTVAGESDIE"
BASE COUNT          348 a          237 c          359 g          278 t
ORIGIN      
        1 acgggaggaa ttgccggaca gtcgatcggc gtgatccggt attctacgaa ttatggtgtg
       61 gtcggttacg agcatgtcgg ctataatgta ggagggattg caggccgcca gtcgggatat
      121 atttatgaat gcaataatga aggaatcata tacggacgca aggatgtcgg cggaattacc
      181 ggtcaggcgg agccgtatat tgttgtggat ttttcaaagg acgttgttta tcagctttca
      241 gaaaatatca acaagctgca tgacctgatc gatcagacac ttcaggatgc ggggtccgaa
      301 tcggatacga tctcaaacag gctgtcggtt ctgcagtcat ttgcagataa ggcgctggac
      361 gatacgtctt acctgtccga taaaacggtt gaatggacgg atggtgtcat agcagctgca
      421 aatgaagccg tcggacgcgc tgagtatatt atggatgaag cgtcaaagga tgacggtctt
      481 cttgatcagt ctgaggatgc ggcggaaaat gtaaaaagtg ctgcaggcaa gctttctgaa
      541 gtcgtagatg atcttgatat ttatcaatat atgagcgatg cggaaaaaga acagtatgac
      601 agggataagt ccagcctgga aagcgcaact tccgaacatg ccgcatatgc agaagatgcg
      661 tataatgcat atgaggacta ttatcttgat gaaatcaggt cctcagaaga taaatacaag
      721 gatccttcgc tgtctgtaaa cgagatcgat ctgcgtccgg tcgtttcagg aaacaccgtg
      781 acagattgga catggaacag ctctaacacg taccagaatt atatggatgt cgaagactgg
      841 gtgcatcata cggcaagtga cggggataaa acatttccgg atccggatac gggagagcag
      901 aatacgcttg acaacgagct gcttgcagat acccagagtg caatggagag caatgcggct
      961 acggtggaaa gtgatgcggc aagctatgcg gattcccagt atctgaccag ccacggttct
     1021 gtttatacaa ccgatataga gagctatgtg gacgggatgg ccggtattgt gctggcgcat
     1081 acgggtgaaa tgtcggacag tgcaaaggag gatgccggca gtgcggcctc atacgcgaag
     1141 agtgcgtctg agaaccttaa gagtgcgagc ggggaagcaa aggatatcgt atcgaccgtc
     1201 gcaggagaat cggatatcga ac
//
LOCUS       DAOSYE010000806         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_107272_length_1219_cov_2.941581, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000806 DAOSYE010000000
VERSION     DAOSYE010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107272_length_1219_cov_2.941581"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PLU43_11710"
     CDS             <1..163
                     /locus_tag="PLU43_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOO29121.1"
                     /translation="AVSETLKAVGLKKELLDSTLRFSFSVETTKEELTYTIRVLKELL
                     PALRKYTRH"
     gene            211..>1219
                     /gene="thiI"
                     /locus_tag="PLU43_11715"
     CDS             211..>1219
                     /gene="thiI"
                     /locus_tag="PLU43_11715"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOO29122.1"
                     /translation="MKYTAFLIKYAEIAIKGKNRYLFEDALVRQIRRALKKTEGEFLV
                     TKTQGRIYIECLSEFDFDETTENLQRVFGITGICPVVHVEDEGFERLAEDVVKYVDDV
                     YPDKNLTFKVYARRARKNYPMDSMEIDRELGGRILDAFPEMKVDVHHPDILLTVEIRE
                     KIYIYSRIIPGPGGMPVGTNGKAMLLLSGGIDSPAAGYMISKRGVKIDAVYFHAPPYT
                     SERAKQKVIDLARIVSAYSGPIYLHVINFTDIQLYIYEKCPHEELTIIMRRYMMKIAE
                     HIAKETDCLGLITGESIGQVASQTMQSLAATNEVCTLPVYRPLIGFDTQEIISISEKI
                     GT"
BASE COUNT          370 a          226 c          322 g          301 t
ORIGIN      
        1 tgcggtcagc gagacattaa aagcggtcgg cttaaaaaaa gaacttcttg attcaacgct
       61 gcgcttcagc ttttccgtag agactacgaa agaagagctg acctatacga tccgtgtatt
      121 aaaggaactg ctcccggcat taaggaaata tacaagacat tagtgcggca ttactgatat
      181 cacggaaaag aagaaaatga ggaaaatggt atgaaatata cggctttttt aattaaatac
      241 gcggaaattg cgatcaaagg gaagaatcgg tatttgtttg aagatgcact tgtaaggcag
      301 atcagacgtg cgctgaaaaa gacggaaggt gaatttttgg ttacaaaaac acagggcagg
      361 atctacatcg aatgtctgag tgaatttgat tttgatgaaa caacggaaaa tctgcagcgt
      421 gtatttggca ttaccggaat ctgtccggtc gtacatgtag aagacgaagg ctttgaacgt
      481 cttgcagagg acgttgtcaa atatgtggac gacgtctatc cggataagaa ccttacattt
      541 aaagtttatg caaggcgggc cagaaaaaat tatccgatgg attcgatgga gatcgacagg
      601 gaacttggcg gaaggatact ggatgctttt ccggaaatga aagttgacgt gcaccatccg
      661 gatattctgc ttactgtgga gatcagggaa aaaatatata tttattcgag aatcattccg
      721 gggccgggcg ggatgccggt cggaacgaac ggcaaagcca tgctgcttct gtccggagga
      781 attgattctc cggcagcagg ttatatgata tccaaaagag gtgttaagat cgatgcggtt
      841 tactttcatg caccgccata tacgagtgag cgtgcaaaac agaaagtgat cgatctggcg
      901 cggatcgtat ccgcgtattc cggaccgatc tatctgcatg ttatcaattt tacggatatt
      961 cagctgtaca tttatgaaaa gtgtccgcat gaagaattga cgatcattat gcgcaggtat
     1021 atgatgaaaa ttgcggagca tattgcgaaa gagaccgact gccttggact gattacgggt
     1081 gagagcatcg gacaggtggc aagccagacg atgcagtcgc ttgccgcaac gaacgaagta
     1141 tgcacgctgc cggtctacag accgctgatc ggttttgata cacaggagat catatcgatt
     1201 tcagagaaga tcggtacct
//
LOCUS       DAOSYE010000807         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_107413_length_1218_cov_3.810834, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000807 DAOSYE010000000
VERSION     DAOSYE010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107413_length_1218_cov_3.810834"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLU43_11720"
     CDS             complement(<1..364)
                     /locus_tag="PLU43_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29123.1"
                     /translation="MKKVVEWIRNLKMISKIRIIIVWLSVFIVLIGAIVYFSLGKIGI
                     GGKDYDEIIMGKDLTADILPPPEYVVESYLTAMEYVMTEDETVREEKVEYIAELKEEY
                     NERHEYWISCQEQGLLSDE"
     gene            750..>1218
                     /locus_tag="PLU43_11725"
     CDS             750..>1218
                     /locus_tag="PLU43_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOO29124.1"
                     /translation="MLDPKITQLLNEQINKEFYSAYLYLDFSNYYAFEGLDGFSNWFR
                     VQTQEERDHAMLMLQYLLNNDEKVILDSVKKPDAVLDSFDAPLKLALEHERMITSSIN
                     QVYAAANEIKDFRTMQFLDWFVKEQGEEEKNSDDLIKKFELFGNDPKSLYMLNS"
BASE COUNT          366 a          279 c          206 g          367 t
ORIGIN      
        1 attcatctga cagaagtccc tgctcctgac aggaaatcca gtactcgtgc cgttcattat
       61 actcttcttt taattcggct atatattcta ctttttcctc tctgacggtt tcatcctccg
      121 tcatgacata ttccattgcg gtcagatacg actcaaccac atattccggc ggcgggagga
      181 tatccgctgt caggtccttg cccataataa tttcatcata atcctttccg ccgataccga
      241 tcttgcccag cgaaaagtac acaattgctc cgatcagaac aataaagacg gataaccaga
      301 caataatgat tctgatctta ctgatcatct tcagatttct aatccactca accacttttt
      361 tcattccttc gcccacctta cattgtaatt tgtaatcctt tattacaaat tatattacgt
      421 tttcatccgc aaatataccg caaactgcaa tgcattctat ttctttcaaa aacacggctt
      481 taatctgcca aaacatttgt atgtgtgaaa aaaaggttaa tttttataag aattctatga
      541 tctgacatac aataaaaaag tttcttatta ctttttcttt cgtttattgc ctgtgtagga
      601 acccacaccg ccagtcccca atcttccgta acacgatcac atggaatttt gccgcaataa
      661 ataaatctgt tgttaccggc cgctgagtat gttattctat ccttgattca tttcaacttt
      721 ttatctgaca gaaaaggagg aacataacaa tgctggatcc aaaaatcaca cagctgctaa
      781 acgaacagat caataaagaa ttttattctg cttatttgta ccttgacttc tccaactatt
      841 atgcttttga aggactggac ggattctcca actggttccg tgtacagaca caggaagagc
      901 gggatcatgc catgctgatg ctgcagtatc ttctgaacaa tgatgaaaag gtcatactgg
      961 acagcgtaaa aaaaccggat gcagtgcttg acagctttga tgctccgctc aagctcgcat
     1021 tggaacacga acggatgatc acctccagta tcaatcaggt ctatgctgcc gcaaacgaaa
     1081 tcaaagactt cagaacaatg cagtttttgg actggtttgt aaaggaacag ggcgaagagg
     1141 aaaaaaattc tgatgatctg atcaaaaaat ttgaactgtt cggcaatgac cccaaaagcc
     1201 tttatatgct gaacagtt
//
LOCUS       DAOSYE010000808         1217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_107601_length_1217_cov_2.929432, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000808 DAOSYE010000000
VERSION     DAOSYE010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107601_length_1217_cov_2.929432"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1217
                     /locus_tag="PLU43_11730"
     CDS             <1..>1217
                     /locus_tag="PLU43_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29125.1"
                     /translation="VSVQNGQLTVGQVFSGEVINIDGNELLLLLGNNQTLSARLDSGL
                     NASVGQILSFEVKSTANNQTSLRPLYANLTQNPSVLNALGEAGMSATNTNISMVSAMM
                     EEGMPVNKNALWNMEKLVNTNQSTDPATIVQLTKLGLPIDELTVGQFQNYKNFEHQIV
                     RDVAALSDGLSKLPAEMMQDGNFSEGIKLAGQILDLLTLPENGSRAQTMENTAVAAKE
                     DGMLQTAEQTQNETIIPQEAADNEKTADLMELSRSLVSETADLETEEPEAKLQKFVFE
                     NLSLLGFDKDEIKQMLKDQNAGDVFLNSIKTLTAAWDGTAGAAADLWDTDKKQLFSEL
                     LSGKEFESLVKDALSKQFVLTPKEVAQEGKVEELYKRITEQSEKIMQILQNAGKDDST
                     AMKSVRNLNNNVN"
BASE COUNT          392 a          241 c          310 g          274 t
ORIGIN      
        1 gtatccgtgc aaaacggaca attgacggtc ggacaggtgt tttccggtga ggtcataaat
       61 atagacggaa atgagctgtt gctgctgctt ggcaataatc agacgctgag cgccaggctg
      121 gacagcggtc tcaatgcatc tgtcggacag atcctgtcct ttgaagtgaa atccacagca
      181 aataatcaga catcactgcg tcctctctat gccaatctga cgcagaatcc gtcagtcctg
      241 aatgctttgg gcgaggcggg catgtctgcg accaatacaa acatatccat ggtatctgca
      301 atgatggaag aggggatgcc tgtcaataaa aatgcgttat ggaatatgga aaaactggtc
      361 aatacaaatc aaagcactga tcctgcaacg attgttcagc tgacaaagct cggcctcccg
      421 atcgatgaac tgacagtcgg gcaatttcaa aattataaaa attttgagca tcagatcgtc
      481 agggatgttg ctgcgctgtc ggacggcctg tcaaagcttc cggcagaaat gatgcaggac
      541 ggcaattttt cagagggcat aaagctggcc ggacaaatac tggatctgct tacactgccg
      601 gaaaatggaa gccgtgcgca gacaatggag aacactgctg ttgctgcaaa agaggacggc
      661 atgcttcaga ctgcagaaca gacacaaaat gaaacaatta ttccgcagga agcggctgat
      721 aatgaaaaga ctgcggattt aatggaactc tccagatctt tggtatctga aacagctgat
      781 ttggagacag aagaaccgga agctaagctg cagaaatttg ttttcgaaaa cttaagcctg
      841 cttggatttg ataaagatga aataaagcag atgctaaagg accagaatgc aggggatgtg
      901 tttttaaaca gtataaaaac attgactgca gcatgggatg gaacagccgg tgcggcggcg
      961 gatctgtggg atacggataa aaagcagctt ttttcagaac tgctttccgg caaagaattt
     1021 gaatcacttg taaaggatgc tttgtcaaag cagtttgtat taacgccaaa agaggttgca
     1081 caggaaggca aagttgaaga gctgtacaaa agaataaccg aacagtcgga aaagattatg
     1141 cagatcctgc aaaatgccgg aaaagacgat agtacagcta tgaaaagcgt ccgaaacctg
     1201 aacaataatg tcaactt
//
LOCUS       DAOSYE010000809         1217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_107605_length_1217_cov_2.833907, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000809 DAOSYE010000000
VERSION     DAOSYE010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107605_length_1217_cov_2.833907"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLU43_11735"
     CDS             <1..861
                     /locus_tag="PLU43_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOO29126.1"
                     /translation="LVVKRGEKASAHSVTEKELLTYGIPFIPMLLMEWLLSSMDKWSI
                     KIFNDFSQTGIYSAAMQIMTILLTFKITYVAFWAPVAMEKYETQPEEVCKAFFADMFR
                     KVQFLCMSAAFLLTICRGIIVLILGERYREAIRIIPFLTLMPVLSILFEMTGQGVKFV
                     GKIKYFNYASLAAIICNLTGNLLLVPKFGGVGAALATAVTYVVYFFTGTFFSKKCYPV
                     NYRFREFIWSLCLYKGYAAFATVTDDQWLSAFVGILLFALHCVFNREVFMDIMRFGTA
                     ALKRIRQNRK"
     gene            886..>1217
                     /locus_tag="PLU43_11740"
     CDS             886..>1217
                     /locus_tag="PLU43_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29127.1"
                     /translation="MKYIAVICLTVFAWILMRWKKQYKKHILGGFVIVMILLSFIYNI
                     RTLSLIVPFYYPVNQTFYQPGFLENGEYPDSFLPLLLNGKTVYTKDDSISIEQCKEEG
                     KSWLYSYY"
BASE COUNT          319 a          230 c          303 g          365 t
ORIGIN      
        1 ctggtcgtca agcgcggtga aaaggcgtcg gcccattccg taacggaaaa agaactgctg
       61 acctatggaa ttccgtttat tccgatgctg ctgatggagt ggctgctttc ttccatggat
      121 aaatggtcga tcaagatatt caatgatttt tcacagacag gtatttattc cgcagccatg
      181 cagatcatga cgatcctttt gacctttaag atcacttatg tggcgttttg ggcgcctgta
      241 gcaatggaaa aatatgaaac acagccggag gaggtctgca aggcattttt tgcggatatg
      301 ttcagaaaag tgcagttttt atgcatgtcg gctgcttttt tgctgacgat ctgcagaggg
      361 atcattgtgc tgatcctcgg cgagcggtat cgggaagcga tcaggatcat tccgtttctg
      421 acattgatgc ccgtactgag cattttattt gaaatgaccg ggcagggagt aaagttcgtc
      481 ggaaagatca aatatttcaa ttatgcttct cttgcggcca ttatctgtaa tctgaccgga
      541 aatctgctgc ttgtgccaaa gttcggcggt gttggggcag cactggcgac ggcggtgact
      601 tatgtggtat atttttttac cggcaccttt ttttcaaaga aatgttatcc ggttaattac
      661 cggttcaggg aatttatctg gtcactctgc ctgtataaag ggtatgccgc gtttgcgacc
      721 gtaacagacg atcagtggct aagtgctttc gttgggatct tactgtttgc actgcattgt
      781 gtattcaata gagaggtctt tatggacatc atgcgttttg gcactgcggc gttaaaacgg
      841 attcgtcaaa accggaaata atacgggaca aataaggagc atgcaatgaa atatatcgca
      901 gtgatatgcc tgactgtttt cgcatggata ctgatgcgat ggaaaaaaca atataagaag
      961 catattcttg gaggctttgt aattgtgatg atattactga gctttattta taatatcaga
     1021 acgctgtcat tgatcgtacc gttctattat ccggtaaacc agacgtttta tcagcccggt
     1081 tttctggaaa acggagaata tccggacagc tttctgcctc ttttgctgaa tggaaaaaca
     1141 gtctatacaa aagatgacag catatcgatc gaacagtgta aggaggaggg aaaaagctgg
     1201 ctgtattcct attatca
//
LOCUS       DAOSYE010000810         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_108005_length_1214_cov_4.518550, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000810 DAOSYE010000000
VERSION     DAOSYE010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108005_length_1214_cov_4.518550"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1214)
                     /locus_tag="PLU43_11745"
     CDS             complement(297..>1214)
                     /locus_tag="PLU43_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29128.1"
                     /translation="RRVDMSYKKRIGIRPAVLIAAGLFLTGLTGCGPSADIIEKMNSA
                     EDLVQNVSAAKDTVLAEESEESGFSGEIDGLPNGFPEEIPIYDGAQILDADSFNGNHY
                     TVLFMTGDSFENVVNYYTEQFALDGTGTGDGEAYYEGIPYGEIMIKGLTIENTGDAVN
                     VYMTLEDGVQAEEIEAEDAVYDSEAEYDGGNSGNMTYENAQEVLLESNYPIELVPLPQ
                     GAKVTESSMIPGCSSGFVDLIMPAAEFEDAVDFYTELGLNAKLMKTDIQETASFNGEI
                     ENLSVDVLVSHLMSAGNDTWIQITVNEND"
BASE COUNT          314 a          325 c          208 g          367 t
ORIGIN      
        1 acaacctgtc ctgtgagata cgttcaaaat aacctaccca gaacggatcc tcaaaaaaca
       61 cctgtaaagt acctgttatt ctgtccatga tcattcctcc ttcatataat acggacagac
      121 cgtacaatta atgaacaaag aacggacaac caaggaggca ggttactgat actcagatga
      181 gtacgtccgg actacctacc ggatctgtat acaaataaga cccgcatacg ttgtgttttt
      241 atctttgcct ctttatacta tcataaattt cgtacgatta cctcattatt tttcaattaa
      301 tcattttcat ttacagtgat ctgtatccag gtatcatttc cagcgctcat caaatggctg
      361 acaaggacat cgaccgacag gttttcaatc tcaccgttaa agcttgcggt ttcctgtata
      421 tctgttttca taagcttcgc attcagacca agctccgtat aaaaatcaac ggcgtcttcg
      481 aattcggctg ccggcatgat caaatccaca aatccgctgc tgcagcctgg aatcatggaa
      541 ctctccgtta ctttcgcacc ctgcggcagc ggaaccagct ctatcggata attactctcc
      601 aataatactt cctgtgcgtt ttcataagtc atatttccgg aatttccgcc atcgtattcc
      661 gcttccgaat catacaccgc atcttccgct tcgatttctt ccgcctgtac gccgtcttca
      721 agcgtcatat agacattgac agcatcgccg gtattctcaa tggtcaatcc ttttatcatg
      781 atttccccat aggggatccc ttcgtaatag gcttctccat cccctgttcc ggtgccgtcg
      841 agtgcaaact gctctgtata ataatttaca acattttcga aagaatctcc ggtcatgaat
      901 aacaccgtat aatgattgcc gttaaaagaa tcggcatcca ggatctgtgc tccgtcataa
      961 atcggaattt cttccggaaa tccgttcggc agcccgtcaa tttcacctga aaaaccactt
     1021 tcttcacttt cctctgccaa aacagtgtct tttgcagcac tcacattttg tacgaggtcc
     1081 tctgctgagt tcattttctc tataatatct gccgacggac cgcagcctgt gagtcctgtc
     1141 agaaataatc cggctgcgat cagtactgcc ggccgtatac ctattctttt tttatatgac
     1201 atatctactc tccg
//
LOCUS       DAOSYE010000811         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_108049_length_1214_cov_2.789474, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000811 DAOSYE010000000
VERSION     DAOSYE010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108049_length_1214_cov_2.789474"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLU43_11750"
     CDS             complement(<1..582)
                     /locus_tag="PLU43_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO29129.1"
                     /translation="MEELLYVEVSNKIESGILSGKLKENDKLSERSLAEGYGVSRSVV
                     RDALKLLNEKGLVTIKTGKGHYVSRPSEADLIVKVGNAIDYSAIPMTQILEAREQLEH
                     AMAGLMTEHVTQEDLTELTRIYGLMEEAVDYAEEFVKQDELFHITLLGAAHNDVLQIF
                     IRALNGMINRNVFLNNREIRMHAQKEHKLMLDAL"
     gene            complement(692..>1214)
                     /locus_tag="PLU43_11755"
     CDS             complement(692..>1214)
                     /locus_tag="PLU43_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOO29130.1"
                     /translation="ELEQEGVRAISSACGFFGNYHDRIAAKINVPTALSSLVQLPWIA
                     ALIKPDQKIGVITADKSSFSDHLLRSCGVPENLWERLVVKDARETKEFCCVIEQRGGW
                     DNTTAREEIVSLGLEIVEEFPNTGAILLECSDMPPYAYAVQAATQLPVFDFITLINWL
                     YQSVSQKPYCGFI"
BASE COUNT          301 a          333 c          218 g          362 t
ORIGIN      
        1 taatgcatcc agcatcagtt tatgctcctt ttgtgcatgc atcctgattt cccggttgtt
       61 caaaaataca tttctgttga tcataccatt caaagcacgg ataaatatct gcaatacgtc
      121 attgtgagcc gccccaagca gtgtaatatg aaataactca tcctgcttca caaattcctc
      181 cgcgtaatcg accgcttcct ccatcagccc atagatcctg gtcagctctg tcagatcctc
      241 ctgtgtcaca tgctctgtca taagtcccgc cattgcatgc tcaagctgtt ctcttgcctc
      301 aaggatctgc gtcatcggga tcgcgctgta gtcaattgca tttcctactt taacgatcag
      361 atcggcctca ctcgggcggc tgacataatg tccttttcct gtcttgatcg taacaagacc
      421 tttttcattc agaagcttca aagcatccct gactacggat cgactgacac catacccttc
      481 cgcaaggctt ctttccgaaa gtttgtcatt ctctttgagt tttccgctta ggattccgga
      541 ttctatttta ttagaaactt ccacgtataa taattcttcc atagcgtttc ctcttttcct
      601 gcattatatc ggatttttta tatttccgca accatacatt ctacaaaatt tatatttttt
      661 tcattcaata aaatacgatt catcagaaat ctcatataaa accgcaatac ggcttctgac
      721 tgacagactg atacagccag ttgatcaatg tgatgaaatc aaagaccggc agctgtgtcg
      781 ctgcctgaac cgcatacgca taaggcggca tatccgagca ttccaaaaga atcgcaccgg
      841 tattcggaaa ttcttccacg atctccagtc cgagggaaac gatctcctcc cttgccgtcg
      901 tattgtccca gccgccccgc tgttcaatta cacagcagaa ttcttttgtt tccctggcat
      961 ccttaacgac cagtctctcc cataaatttt ccggcactcc gcagcttctc agcagatgat
     1021 ccgagaacga cgatttatcc gccgtgatca cacctatttt ctggtccggc tttataagcg
     1081 cggcgatcca tggaagctgc acaagagaag acagtgctgt cggaacgttt atttttgctg
     1141 cgatcctgtc atgataattt ccaaaaaatc cgcaggcaga ggagattgca cggactcctt
     1201 cctgctcaag ctcc
//
LOCUS       DAOSYE010000812         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_108209_length_1213_cov_2.552677, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000812 DAOSYE010000000
VERSION     DAOSYE010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108209_length_1213_cov_2.552677"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLU43_11760"
     CDS             complement(<1..1107)
                     /locus_tag="PLU43_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO29131.1"
                     /translation="MERLKRGIKRKVSYFSNENVPIEDKNFYLTVLYAIPATLAMTVV
                     FFCSKMDIRMVYLSLFLLLFMFLILYIAYRYGEYQLYTVVFCVMINCFVYPIFYFLTG
                     DIFNGMPLYFASSIIMTFFLLKGRLMFGLVSTEIVWYAFIIFFSYIYRERLEAYHKIL
                     EYGNEIAISFLLASFIPIFIIRYQTSIYHRIKENTTEASRVINSAKISKSRFLANMTH
                     EIRTPMNAIVGMNELILKEGLNPAAMEQAETIKEASAQLLTIINNILVYSKLDSNKME
                     LLQTKYSFRELMMGIIHTVSMEYAAEETEFQVFIDRNIPVYLYGDDIRIRQVFMYLLF
                     SSMHQLPHGRMSLSVTGEKNPADHTVKLKCRVAET"
BASE COUNT          385 a          265 c          193 g          370 t
ORIGIN      
        1 ggtctcggca accctgcatt tcaattttac cgtatgatcc gcaggatttt tctctcctgt
       61 cactgaaagt gacattctcc catgaggcag ctgatgcatg gaactaaaca gcagatacat
      121 aaatacctgt ctgatcctga tgtcgtctcc atacaggtaa actggtatat tcctgtcaat
      181 aaacacctga aattccgtct cttccgccgc atattccatg gacaccgtat gaataattcc
      241 catcatcagc tctctaaagc tgtattttgt ctgcagcagt tccattttat tggaatccag
      301 cttagagtat acaagaatat tattgataat tgtcagaagc tgtgcagaag cctctttgat
      361 cgtttccgcc tgttccatag ccgccggatt caggccttcc ttcaaaatca gttcattcat
      421 tccgacgatt gcattcatcg gcgtcctgat ttcatgtgtc atatttgcaa gaaatctgct
      481 tttactgatc tttgccgaat taattacacg gctcgcctcc gttgtatttt cttttatcct
      541 gtgataaatt gacgtctgat acctgataat aaaaatcgga ataaatgatg ccaaaaggaa
      601 gctgatcgca atttcattgc catactccag tattttatga tatgcttcaa gtctttccct
      661 gtatatatag gaaaaaaaaa taataaacgc ataccagacg atctccgtac ttaccaggcc
      721 aaacatcagt ctgcccttta aaagaaaaaa tgtcataata atactggacg caaaataaag
      781 cggcattcca ttgaaaatat cacctgtcag aaaataaaat atcggataaa caaaacaatt
      841 gatcataacg cagaatacaa ctgtatacag ctgatattcc ccatagcggt atgcaatata
      901 caaaatcaga aacataaaca aaagcaggaa caaagacagg tataccatac ggatatccat
      961 tttagaacaa aaaaatacga ccgtcatagc cagcgttgcc ggaatggcat ataatactgt
     1021 cagataaaaa tttttatctt ctattggaac attttcattt gaaaaatacg aaaccttccg
     1081 tttgattcct cttttaagtc tttccatata gtcagcagtc tcctatttat tctttccgac
     1141 aatcagattt ttaggaataa atgtcttcag cgcacgctcc attttatcaa gttctatcgg
     1201 tttggatata taa
//
LOCUS       DAOSYE010000813         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_108931_length_1208_cov_3.106678, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000813 DAOSYE010000000
VERSION     DAOSYE010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108931_length_1208_cov_3.106678"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLU43_11765"
     CDS             complement(<1..390)
                     /locus_tag="PLU43_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOO29132.1"
                     /translation="MDSQTVKQMLDFIELSPSRYHVVAGMKKELREAGYSELFESDRW
                     KLCAGGKYFVVRNDSAILAFQIPKTDFRGFMIMASHSDSPAFKIKENPEILVEAAYTK
                     LNVEKYGGALLAQWLDRPLSVAGRIFVK"
     gene            complement(418..>1208)
                     /locus_tag="PLU43_11770"
     CDS             complement(418..>1208)
                     /locus_tag="PLU43_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOO29133.1"
                     /translation="PGIWMKLLTILTFVLMLAVNTLAVVLPINGVSTGQAADAYPNLF
                     TPAGITFSIWSLIYILLAAHVLYVLGLFREKETGIDVQIFRRICVTFSISSAVNAAWI
                     FSWHYGKILLSMLLMLILLNCLIKINVMISKQTLSAKERFFVRLPFTVYFGWITIATI
                     ANAAVLLVSVGWDRFGLSENIWTVIMLLAGMLIGLAVLLRFKTVSYSLVLIWAYTGIL
                     IKHTSVSGFAGQYPAVIITAGICLALFAASAGYLLLHFSLKGGQ"
BASE COUNT          369 a          301 c          234 g          304 t
ORIGIN      
        1 ttttacaaag atccgccctg caacagacag cggtctgtcg agccactgtg caagcagcgc
       61 gcctccatac ttttccacat tcaatttcgt atatgcagct tccactaaaa tttccgggtt
      121 ctctttgatc ttgaatgccg gagaatcact gtggcttgcc atgatcataa atcctctaaa
      181 atctgttttt ggaatctgaa acgccagaat ggcggaatcg tttctgacca caaaatattt
      241 tccgccggca cacagcttcc atctgtcgct ttcaaaaagc tcagaataac cagcctccct
      301 taattctttt ttcattcctg ccactacatg atagcggctt ggacttaact caataaaatc
      361 aagcatttgt tttacagtct gactgtccat gtacaactcc tttatccttt ctctgattta
      421 ttgtccgccc tttaaggaga aatgcagcag cagatatccg gcagatgcag caaaaagcgc
      481 aagacagata ccggccgtta tgatcactgc aggatactgt ccggcaaatc cggaaacgga
      541 cgtatgtttt atcaaaatcc ccgtataagc ccaaataagt acaagactat aagaaacggt
      601 tttaaagcgc agcaataccg caagtccgat cagcatccct gcaagcagca tgatgaccgt
      661 ccatatattt tccgataatc cgaaacgatc ccagccgaca cttacgagga gtaccgcagc
      721 atttgcgatc gttgcaatcg tgatccagcc aaaataaacg gtaaacggaa gccgcacaaa
      781 aaatcgttct ttcgctgaca gggtctgttt gcttatcatg acattgatct tgatcagaca
      841 attcagtaaa ataagcatca gaagcatgga taacaatatt ttcccataat gccatgaaaa
      901 tatccatgcg gcattcactg cggaagagat agaaaatgtg acacagatcc tgcgaaagat
      961 ctgcacatca atgcccgttt ctttttctct gaaaaggccg agtacataca atacatgcgc
     1021 agccagaaga atataaataa gagaccagat ggaaaacgtt atcccggccg gtgtaaacag
     1081 attgggatac gcatccgcag cctgccctgt cgacactcca ttgataggca gcacgacagc
     1141 aagcgtattg acggcaagca ttaatacaaa cgtaagaatc gtcagcagtt tcatccatat
     1201 accaggtt
//
LOCUS       DAOSYE010000814         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_109237_length_1206_cov_3.307559, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000814 DAOSYE010000000
VERSION     DAOSYE010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109237_length_1206_cov_3.307559"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1206
                     /locus_tag="PLU43_11775"
     CDS             <1..>1206
                     /locus_tag="PLU43_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29134.1"
                     /translation="FFQKNVLFGIDTRAQLLRIEGIFEGLQEGQFPVIIDPNYCNEYG
                     QLGVLYPNLFLYIPALLRYFGISLEVSYRFFMILVNAMTVLIMFVSAKSLFHSSRWAL
                     ASALFYCLEPYHLYVMLESGNGAGRGVAMAFLPMIFVGIYYIAIQNRKGWRWLAIGMW
                     GIFSSHIIITFLAVGFLFVFCICNIKKFIHKAALLDLIKAIGLFGVLSAGTLLPFADF
                     YFTDWNRQALEWSGFYGLQVHAGDIIRTPYSLLTLLFLFLAGVLFIVAYRKKLICESY
                     YLKLYIAAVLLYFMTTAAMPWRLLGRFHIMNDLLNMIQVGHRFQTLISPIEAFLAGKL
                     LQVFHHKKHTIYLYFLLGILLYCSLVCSYRIYFREGPLLPDAVTGNINSQFPEDYLPA
                     GTKTEYYATN"
BASE COUNT          319 a          183 c          259 g          445 t
ORIGIN      
        1 ttttttcaga agaacgtatt atttggaatt gatacaagag cgcagctttt aagaattgaa
       61 ggcatatttg aaggtctgca ggaaggtcag ttcccggtca taatagatcc aaattactgt
      121 aatgaatatg gacaattggg ggtattatat ccaaatttat ttttatatat accggcgctt
      181 ctaagatatt ttggaatttc tcttgaagtc agttatcgtt tttttatgat acttgtaaat
      241 gcgatgaccg tattaattat gtttgtatct gcaaaaagtc tttttcattc ttcgagatgg
      301 gcattggcgt cagctttgtt ttattgcttg gaaccatatc atctgtatgt catgctggaa
      361 agcggaaatg gagccggacg tggtgttgcc atggcttttc ttccaatgat ctttgttggg
      421 atttattata ttgcaatcca aaacagaaaa ggttggcgct ggctggcaat cggcatgtgg
      481 ggcatttttt catcgcacat tattataacc tttttagcag taggttttct ttttgttttt
      541 tgtatctgta atataaaaaa atttatacat aaagcagctt tgcttgattt aataaaagcg
      601 atcggtcttt ttggcgtatt gtctgcagga acactgcttc catttgctga tttttatttt
      661 acggactgga atagacaggc attggaatgg agcggttttt atggactgca ggtccatgca
      721 ggagacatta tacgtactcc ctatagtctg ctgactcttt tatttttatt tttagcagga
      781 gtattgttta ttgttgcata tcgaaagaaa ctaatttgtg agagttatta tctgaaattg
      841 tatatagcag cagtactttt gtattttatg acaactgcgg caatgccatg gaggctgctt
      901 ggccggtttc atataatgaa tgatctgctg aatatgattc aggtcggtca tcggtttcag
      961 acgttaattt ctcctataga agcgtttttg gcgggaaaat tattgcaggt ctttcatcat
     1021 aaaaagcata cgatatatct ttattttttg cttggaattt tattatactg cagtcttgta
     1081 tgcagttatc gaatctattt tagagagggg cctcttttgc cggatgccgt gaccggaaat
     1141 attaattcac agtttccgga ggattatttg ccggcaggta caaagacaga atattatgct
     1201 actaat
//
LOCUS       DAOSYE010000815         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_109455_length_1205_cov_2.199130, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000815 DAOSYE010000000
VERSION     DAOSYE010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109455_length_1205_cov_2.199130"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLU43_11780"
     CDS             complement(<1..695)
                     /locus_tag="PLU43_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29135.1"
                     /translation="MKKKLIALLAAAAMVVSFTGCGSEASSTADTAAADSSAKEEASG
                     DNSLTVWCWDPAFNIYAMEQAAEVYKQDNPDFVLNVVETPWSDLQTKLTTAASSGDLS
                     TLPDILLMQDNAFQKNVISYPDAFTDLTDAGIDFTQFGSSKVAYSTVDGKNYGVPFDN
                     GAVIACYRTDILEQAGYSVDDLTDITWSQYIEIGKDVLAKTGKPLLSCQAGETDVIMM
                     MLQSAGGSLFDEDG"
     gene            complement(907..>1205)
                     /locus_tag="PLU43_11785"
     CDS             complement(907..>1205)
                     /locus_tag="PLU43_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO29136.1"
                     /translation="DACHMSETHFRRQFGKNMNMSPIEYINLVRVQKACELLNKTNYS
                     METVAEKTGFRTVSTFNRNFNHVVGSSPYHWKTHFKNYEKKTQEFKISAHKGWE"
BASE COUNT          309 a          294 c          220 g          382 t
ORIGIN      
        1 ccgtcttcgt caaacagaga acctcctgcc gactgcagca tcatcataat tacatccgtc
       61 tcgcctgcct gacaggaaag cagcggtttg ccggtctttg caagtacatc cttgccgatc
      121 tcaatatatt gggaccatgt gatatccgtc agatcgtcca cactgtagcc tgcctgttca
      181 agaatatctg ttctgtagca ggcgattacg gcaccgttat caaacgggac accataattt
      241 ttaccgtcca cggtcgaata ggcaactttt gaagagccaa actgcgtaaa atcaattccc
      301 gcgtccgtca gatccgtaaa ggcatccgga taactgatca catttttctg aaatgcattg
      361 tcctgcatca gaagaatatc gggaagtgta ctcagatctc ccgaggaagc agctgttgtc
      421 agcttcgtct gcaggtcact ccatggggtc tccactacat taagtacaaa atccgggtta
      481 tcctgtttgt aaacttctgc ggcctgctcc attgcataaa tattaaacgc aggatcccag
      541 caccagaccg ttaatgaatt gtcgccggat gcttcttcct ttgccgaaga atcagcagca
      601 gccgtatcag ccgttgatga tgcttccgac ccgcacccgg taaaagatac taccattgct
      661 gccgctgcaa gtaatgcaat caatttcttt ttcattttca ttcctcctga atcgtttttc
      721 aaccgatttt tactgtgttc cgttttcctg ttcttcgaag ttttgtacaa tatgcatatt
      781 ttttttaaca aatcctttat ctattgtgtg attgtgtaca tattatcaca agggttttga
      841 ccggattgtt aagaatttcg cttatataat tcgcaatatc gaccattttt tcaattgcgc
      901 aaacggttat tcccatcctt tatgcgcaga aattttaaat tcctgcgttt ttttctcata
      961 attcttaaaa tgcgtcttcc aatgataagg agagctgcct acaacatggt taaaattcct
     1021 gttaaaagta gatactgtcc gaaatcccgt tttttctgca acagtttcca tggaataatt
     1081 tgttttattc aaaagctcgc atgctttctg cacccgtaca agattgatat actctatcgg
     1141 actcatattc atatttttcc cgaactgccg tctgaaatgc gtttcactca tatgacacgc
     1201 atccg
//
LOCUS       DAOSYE010000816         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_109581_length_1204_cov_2.730200, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000816 DAOSYE010000000
VERSION     DAOSYE010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109581_length_1204_cov_2.730200"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..556
                     /locus_tag="PLU43_11790"
     CDS             221..556
                     /locus_tag="PLU43_11790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOO29137.1"
                     /translation="MEQKEKNSFQLIYEVVQKIPKGRVASYGQVAALAGNRRWARVVG
                     YALHVNPDEQAIPCYRVVTKEGYTAPGFVFGGPGKQQELLEADGIEFVEGHVDMRKYQ
                     WDTASLYEE"
     gene            671..>1204
                     /locus_tag="PLU43_11795"
     CDS             671..>1204
                     /locus_tag="PLU43_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO29138.1"
                     /translation="MIRIITDSTSDISMEKAKELNVDVMALTVSFGEQSYKSGIDITN
                     AEFYKRLVSSSELPKTAQVNPYEFEEKYKEYLDAGDEIISIHISKELSGTYQSAVIAK
                     EALDSDKIAVLDSLNVTSALGLLVIIAAKMRDEGASMQEITDALHSYIPRLRLFVVLD
                     TLDYVRKGGRISPTIAFI"
BASE COUNT          387 a          212 c          293 g          312 t
ORIGIN      
        1 aaattcttat ttttaacaac ttttctagag ccatacaaaa taccatattc ttttagatgc
       61 cggctgattg ctgttttcat ttctgcctcg gttgtgtata ataatgtatt gaaatgtaaa
      121 agacactaag aatactttaa catatttttc ggaccggatg caagcatata caaggcagac
      181 tgtttgtttg gttctgtgtt cgaaacagtc aggagggaaa atggaacaga aagaaaaaaa
      241 cagctttcag ctgatctatg aggtcgtgca gaaaataccg aaaggccgtg ttgcatccta
      301 tggacaggtg gctgccctgg caggaaaccg gcgctgggcg cgagtggtcg gttatgcact
      361 gcatgtaaat ccggatgaac aagcgattcc atgctacagg gtcgtgacaa aggaagggta
      421 tacagcgccg gggtttgtat ttggaggacc cggaaaacag caggagctgc ttgaagcgga
      481 cggaattgaa tttgtggaag ggcatgtaga catgcgaaaa tatcaatggg atacggcttc
      541 tctttatgag gaataaattc ataaaaaacg aaaaaaagaa ttgacgaata tagggaaaac
      601 acgtagacta ataaaagaac aacagcatgt agtttacaaa accatatcac ataaggaaag
      661 ggatattatc atgatcagga ttattacgga cagtacgagc gatatttcga tggaaaaagc
      721 aaaggaatta aatgttgatg tcatggcatt gacagtaagc tttggtgagc agtcttataa
      781 aagcggaatc gatattacta acgcggaatt ttataaaaga cttgtgtctt ccagcgaact
      841 gcctaaaaca gcacaggtaa atccctatga atttgaagaa aaatataaag aatatctgga
      901 tgccggagac gagatcatca gtatccatat ttcaaaggaa ctgagcggaa cgtatcagtc
      961 ggctgtcatt gcaaaagaag cacttgactc ggataagatt gctgtgctcg attcgctgaa
     1021 cgtgacaagc gcacttggtc tgcttgttat tattgcggca aagatgcgtg acgaaggcgc
     1081 gagtatgcag gagatcacag atgcgctgca ttcttatatt ccccgtctgc gtttgtttgt
     1141 cgtactggac acacttgact atgtaagaaa aggcggcagg atctctccta cgatcgcttt
     1201 tatc
//
LOCUS       DAOSYE010000817         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_109742_length_1203_cov_2.701220, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000817 DAOSYE010000000
VERSION     DAOSYE010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109742_length_1203_cov_2.701220"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLU43_11800"
     CDS             <1..481
                     /locus_tag="PLU43_11800"
                     /inference="COORDINATES: protein motif:HMM:NF040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HOO29139.1"
                     /translation="CMVARKQNPFAMLIKVIVYAFAGFFCVLGIIGFTYMFIPGLALI
                     AAGYFLLPNLDLEFEYLYLDKEITIDKVMAKQKRKRVMVLDLNKMEIMAPVTSHELDS
                     YKARKVPMKDYGSKMPDSKLYAIVYHDQNKEELVCIEPNTEMLRAIKTVFPRKVIEY"
     gene            592..>1203
                     /locus_tag="PLU43_11805"
     CDS             592..>1203
                     /locus_tag="PLU43_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29140.1"
                     /translation="MKLFLVEKIRSKKLVTGKRVFVFFSFVVFAAFVYLFFSDHKDIY
                     CIDDQSEMITSNTDLETLRYALSKGHYEIHLNAVVPLQTPVVVYIDSEHQLQTSLEVN
                     EAGGDYTIIFDLDETTSLFHLIFLNGAVSGLQINQYDITSEDAFSNDQVFFMFLCMLA
                     AIGAYFIFSSRCYHNMDMHKKLYIFVTIGIAVFVSYPMFKNYLG"
BASE COUNT          339 a          199 c          277 g          388 t
ORIGIN      
        1 atgtatggtg gcaagaaaac aaaatccgtt tgctatgctg atcaaggtta tcgtatacgc
       61 gtttgcgggt tttttctgtg ttttgggtat tatcggattt acgtatatgt ttattccggg
      121 tctggctctg atcgcagcgg gctattttct ccttccgaat ctggatctgg agtttgaata
      181 tctctatctg gataaggaga tcacgatcga taaggtgatg gcaaagcaga aacggaaaag
      241 ggtcatggtc cttgatttga ataaaatgga gatcatggcg ccggtcactt cccacgaact
      301 ggattcttat aaggccagaa aggtaccaat gaaggattat ggttccaaaa tgccggacag
      361 caagctttat gcgatcgttt atcatgacca gaataaggaa gagctggtct gcatcgagcc
      421 taatacagaa atgctgcggg cgatcaaaac cgtatttcca cggaaagtaa ttgaatatta
      481 gcggttaaat aaatagaaat gggttttgta taaaagataa agcttatttc tatttttaat
      541 agcagtattt tgatgcatga atgaaaataa gagccgcatt ggagagccgg aatgaaatta
      601 tttctcgtag aaaagattcg cagcaaaaaa cttgtaacag ggaagcgtgt ttttgtattt
      661 ttttcttttg ttgtttttgc tgcttttgta tatctgtttt tttctgatca taaggacata
      721 tactgtatag acgaccagtc ggaaatgata acaagcaata ctgatttgga aacgctgcgc
      781 tatgcgctgt ctaaagggca ttacgagata catctgaatg cggtggttcc gctgcagaca
      841 cctgtagttg tctacattga ttcggaacat cagctgcaga cttctcttga agtaaacgaa
      901 gcgggcggtg attatacgat tatttttgat ctggatgaaa ccaccagcct gtttcatctt
      961 atttttttaa acggggcggt cagtggtctt cagatcaatc agtatgatat cacaagtgaa
     1021 gatgctttca gcaatgatca ggtgtttttt atgtttcttt gtatgcttgc ggcaataggc
     1081 gcatatttta ttttcagcag ccgctgttat cataacatgg atatgcataa aaaattatac
     1141 atctttgtga cgatcggaat cgctgttttt gtcagctatc cgatgtttaa gaattattta
     1201 ggc
//
LOCUS       DAOSYE010000818         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_110107_length_1201_cov_2.407504, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000818 DAOSYE010000000
VERSION     DAOSYE010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110107_length_1201_cov_2.407504"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLU43_11810"
     CDS             <1..295
                     /locus_tag="PLU43_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOO29141.1"
                     /translation="NENWLRRKYNVVKETETSSLYLYTKLKKTERTMQDIHLDERDYA
                     NAGGGFPIRIEDVGVIGAILVSGLNHVADHDLIVKCLAKYLHTDEVPRIRKMN"
     gene            complement(204..1199)
                     /locus_tag="PLU43_11815"
                     /pseudo
     CDS             complement(204..1199)
                     /locus_tag="PLU43_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
BASE COUNT          339 a          248 c          302 g          312 t
ORIGIN      
        1 aaacgaaaac tggctgaggc gaaaatataa cgttgtaaaa gaaacggaaa caagcagtct
       61 ttatttatat acaaaactga agaaaacaga gcgcacgatg caggatattc atctggacga
      121 aagagattat gccaatgccg gcggaggatt cccgatccgt atcgaagatg tcggtgtgat
      181 cggagcgatc cttgtatccg gtcttaatca tgtggctgat catgatctga tcgtaaagtg
      241 tctggcgaaa tatcttcata ctgatgaggt gccgagaatt cgcaaaatga actgacagaa
      301 tccatgggaa aatatttgcc catgtccggc ggcagcttgt ccgtaaagta cattggctct
      361 tttgtcagcg gatgctggaa tgaaagacag gcagaatgca gagcctgtct ttttataatg
      421 gaataatccg gatgatacag aaaatcaccc ggcagaggat gtccgatata acgcatatga
      481 acacggatct gatgcgtgcg tcatgtttcc agacagacac gcagcaggga atattttttt
      541 tcggttccgt ttatttttag gctgatcgaa gagagcactt cgtaataggt aacggcagca
      601 tccccatttt tgaaatcaac agtacgctcg atcgcagaac cgcattttct ggcgatcggc
      661 gcccgtatcg tgccggagat atcaggaatt ccttctgtaa tagccaggta ttcccgtctg
      721 atcagcctgt tttttgccat gtcagacagt aaggaagcgc tgagcatgtt tttggcaatg
      781 atcgttaacc cgctcgtatc tctgtccagc cggttggaac agcggaaaac aaagggcagg
      841 tttctgcttt gataataata tgccacggca tttgccagag aattgtcatg attattcatg
      901 gaaggatgga tcggcatatc ggagggcttg tcaacgatca gaatatcctc gtcttcatag
      961 aggacagaaa gcggcagggg aacaggcgtg atattttcag aagatgcttc ttccttcaaa
     1021 aatacggaaa gagactctcc taagtgcagt acatagttca gatataccgg cacaccatcc
     1081 gcacaaatac ccatcccggt gtttttgaga tgggtaagta tatggcggga atagccgcgc
     1141 tgctgtaaaa actgccgtac ggtcagtgcc tgttcggttt ctgttatcgt gtaatggata
     1201 t
//
LOCUS       DAOSYE010000819         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_110386_length_1199_cov_2.654720, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000819 DAOSYE010000000
VERSION     DAOSYE010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110386_length_1199_cov_2.654720"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLU43_11820"
     CDS             complement(<1..531)
                     /locus_tag="PLU43_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29142.1"
                     /translation="MSLIVDHVSYIYSRETAYEIKALDDISLQIGDGEFIGLIGHTGS
                     GKSTFVQHLNGLLRATSGNIYYNGEDIYEKDFSMKKLRSKVGLVFQYPEHQLFEVDVF
                     SDVCYGPKNLGLSKAEAELRAYEALKQVGVADEYFYQSPFDLSGGEKRRVAIAGVLAM
                     RPEVLILDEPTAGLDPK"
     gene            complement(516..>1199)
                     /locus_tag="PLU43_11825"
     CDS             complement(516..>1199)
                     /locus_tag="PLU43_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO29143.1"
                     /translation="KHMNAILYPTEGTIFVDGKDTSDLENMWDVRQTTGMVFQNPDNQ
                     IIGTVVEEDVGFGPENMGVPTKEIWERVEESLKAVGMYKYRKHAPSKLSGGQKQRVAI
                     AGVVAMHPKCIVLDEPTAMLDPKGRKEVIRAVRALNDVERITVILITHYMEEVIYANK
                     VFVMDEGKIVMQGTPREIFSRVEELKEIRLGVPQATLLAYELKREGLELPDGILSADE
                     LVEALCRLS"
BASE COUNT          293 a          355 c          215 g          336 t
ORIGIN      
        1 cttcggatca agtcctgccg taggctcgtc taaaatcaat acttccggcc gcatggccag
       61 cacacctgca attgccacac gtcttttttc tccgcctgac aagtcaaacg gcgactgata
      121 gaaatattca tccgcaacgc cgacctgctt taatgcctca tatgccctaa gttcggcttc
      181 tgctttggaa aggccgagat ttttcggtcc gtagcagaca tccgaaaaca catctacttc
      241 aaacagctgg tgctccggat attgaaagac aaggcctacc ttgctgcgca gctttttcat
      301 ggaaaaatcc ttttcataaa tatcctctcc gttgtaataa atatttccgg aggtagcgcg
      361 caaaagccca ttcagatgct ggacaaatgt tgattttcca gaacccgtat gcccgatcag
      421 accaataaat tccccatccc cgatctgcag gctgatatca tcaagtgcct ttatttcata
      481 tgcggtttcc ctgctgtata tataactgac atgatctacg ataagcgaca taatgcctca
      541 accagttcgt ctgcggacaa aattccgtcc ggcagttcca atccttccct ttttaattca
      601 tacgccagta atgttgcctg cggaacaccg agccggatct cctttaactc ttccactctt
      661 gaaaaaatct cccttggcgt tccctgcata acgattttcc cttcatccat tacaaatact
      721 ttattcgcat agatgacttc ttccatgtaa tgcgtaatca aaatgaccgt tatccgttca
      781 acgtcattca atgccctgac cgcacggatc acctctttcc gtcctttcgg atcaagcatt
      841 gccgtaggct cgtcaagcac gatacatttc ggatgcatcg ccacgacacc cgcaattgcc
      901 acacgctgtt tctgaccgcc ggaaagcttg cttggggcat gcttgcggta tttatacatg
      961 ccgaccgctt tcagactctc ttccacacgc tcccagatct cctttgtcgg cactcccata
     1021 ttttccgggc caaacccgac atcttcctca acgaccgttc caatgatctg attatccgga
     1081 ttctgaaaga ccattcctgt cgtctgccgg acatcccaca tattctcaag atcggacgta
     1141 tcctttccgt ccacgaatat cgttccctca gtaggataca aaatcgcatt catatgctt
//
LOCUS       DAOSYE010000820         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_110938_length_1195_cov_3.957018, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000820 DAOSYE010000000
VERSION     DAOSYE010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110938_length_1195_cov_3.957018"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1134)
                     /locus_tag="PLU43_11830"
     CDS             complement(43..1134)
                     /locus_tag="PLU43_11830"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004062113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO29144.1"
                     /translation="MLQHTDHFHGSDLEKIEEIYGIKKEDIISFSANVNPLGLSTKLR
                     TSLAAHLDVITSYPDREYTVLRKTIAAYCGSEYEHILVGNGSTELISLFIQMEHPKKA
                     LIIGPTYSEYEREVSLGGGTSLYFPLKESADFKLDLSELKSQLNESIDLLVLCNPNNP
                     TSSAITRSTMRSILDICKQHDIFVMIDETYIEFADNYDEINAVPLTDVYNNLIILRGI
                     SKFFAAPGLRLGYAVTGNMDLIKTINSHKNPWTINSLAETAGQLMFTDTDYIRKTKAL
                     ISSERTRIVTHLKENKDFKIYEPPANFVLVRILREDLNADLLFDKAIRQNMMIRNCAS
                     FPFLSNKYFRFCFMMPKANDRLLSCIEDA"
BASE COUNT          310 a          239 c          294 g          352 t
ORIGIN      
        1 acactgcttt ctaaaaagga agtatacgaa gcggagtttt gatcaggcgt cttcgataca
       61 gcttaacagc cggtcgtttg cttttggcat cataaagcag aaccggaaat acttgttgct
      121 aagaaacggg aaagaagcac agttccggat catcatgttc tgccggatgg ctttgtcgaa
      181 cagaagatca gcgttcagat cttcccgcag tattctgaca agtacaaaat tagcgggcgg
      241 ctcatatatt ttaaagtcct tattttcttt gagatgcgtt acgatacgcg tacgttcgga
      301 ggagataagg gcttttgttt tccgaatgta gtccgtatcg gtaaacatca gctgtccggc
      361 tgtttccgca agggaattga tcgtccatgg atttttgtgg gaattgatcg ttttgatcag
      421 atccatattg cctgtaacgg catacccgag acgcagtccc ggagcagcaa aaaatttgga
      481 aataccgcgc agaatgatca ggttgttata gacgtctgtt aaggggaccg cattgatttc
      541 atcgtaatta tcggcaaatt caatgtaggt ctcgtctatc atgacaaaaa tatcatgctg
      601 tttgcagata tcaagaatag aacgcatcgt ggaacgggtg atcgctgagg atgtcgggtt
      661 atttgggttg cagagaacca gcagatcaat gctttcgttc agctgtgact tgagctccga
      721 aaggtcaagc ttgaaatccg cagattcctt taacggaaag tacaggctcg tgccgccgcc
      781 aagtgacacc tcacgttcgt attcggagta ggtgggtccg atgatcagtg ccttttttgg
      841 gtgctccatc tgaataaaaa gggagatcag ttccgtggag ccgttgccga caagaatgtg
      901 ttcgtattcg gaaccgcagt atgcggcaat cgttttgcgc agaaccgtat attccctgtc
      961 agggtagctt gtaatgacgt caaggtgcgc ggcaagagag gtccttagtt ttgtggaaag
     1021 cccgagcgga ttgacatttg ctgaaaagct tatgatatcc tcttttttaa tgccgtaaat
     1081 ttcttcgatt ttttctaaat cacttccgtg aaaatgatcg gtatgctgta acatattttc
     1141 cctcttttct atatgttttc gttgcggata acaacgaaat catgttatac tttat
//
LOCUS       DAOSYE010000821         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111106_length_1194_cov_4.136084, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000821 DAOSYE010000000
VERSION     DAOSYE010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111106_length_1194_cov_4.136084"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="glnA"
                     /locus_tag="PLU43_11835"
     CDS             <1..925
                     /gene="glnA"
                     /locus_tag="PLU43_11835"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOO29145.1"
                     /translation="FHTDENGLPTTVTHERAGYFDLGPVDLGENARRDMVMTLEDMGF
                     EVEASHHEVAPAQHEIDFKYDEALITADNIMTFKLAVKTIAKRHGLHATFMPKPKFGI
                     NGSGMHTNMSLQKDGKNIFFDPEDQLQLSKEAYYFIGGIIKHMKGMTAVTNPIINSYK
                     RLVAGYEAPVYIAWSATNRSPLIRIPASRGAGTRIELRCPDPSCNPYLVLALCLAAGM
                     DGIKNQNMPPKSVDLNIFEMTEEERAFYNIEALPVNLYDAVCEMEKDPFIKDVLGEHI
                     FDKYVEAKKKEWDEYRTQVTQWELDEYLYKI"
     gene            969..>1194
                     /locus_tag="PLU43_11840"
     CDS             969..>1194
                     /locus_tag="PLU43_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination regulator"
                     /protein_id="HOO29146.1"
                     /translation="MVSVIVVFPKQEDAMNIRRLLVQNGIDVIGVSTTGAQAIQQMDG
                     LDGGVVVCGYKFSDMMYSDLHDFLPPNFEML"
BASE COUNT          337 a          227 c          323 g          307 t
ORIGIN      
        1 gttccacacg gatgaaaacg gacttccgac aacagtgaca catgagcggg ccggctattt
       61 tgatttagga ccggtggatc ttggtgagaa tgcacgccgg gatatggtca tgactcttga
      121 ggatatgggt tttgaagtgg aggcatctca tcatgaggta gcacccgcac agcatgagat
      181 cgattttaag tatgatgaag cgctgatcac ggcagataat attatgacat tcaagcttgc
      241 tgtcaagacg atcgcaaaac gccacggcct gcatgcgact tttatgccaa agcccaaatt
      301 cggcatcaat ggttcgggga tgcatacgaa tatgtcattg caaaaagacg ggaaaaatat
      361 ttttttcgat ccggaagatc aattgcagct cagtaaggaa gcgtattatt ttattggcgg
      421 catcattaag catatgaagg gcatgacagc agtcacaaat cctattatca attcctataa
      481 gcgtcttgtg gcaggctatg aggctcctgt ctatattgca tggagcgcaa cgaacagaag
      541 cccgctgatc cgtattccgg cttcaagagg cgcaggtacg agaatagagc ttcggtgccc
      601 ggatccgtcc tgtaatccgt atctggtgct ggcgctttgt cttgcggcag ggatggacgg
      661 aataaaaaat cagaacatgc cgcctaagag cgttgatctg aacatcttcg agatgacgga
      721 agaggaaagg gccttctaca atattgaagc gcttcctgtt aatttatatg atgctgtctg
      781 cgagatggaa aaagatccgt ttattaagga tgtgctcggt gagcatattt ttgataaata
      841 tgttgaggcg aagaaaaaag aatgggacga atatcggaca caggtaacgc agtgggaatt
      901 ggacgaatat ttatacaaga tttaggcatg acttccaaag cggataaacg atcagaggag
      961 gtgtgtgctt ggtaagtgta attgtagtat ttccaaagca ggaagatgcg atgaatatcc
     1021 gcaggctgct tgtacagaac ggtatcgacg tcataggcgt cagtacgacg ggagcgcagg
     1081 cgattcagca aatggatgga cttgacggcg gggtcgttgt ctgcggttat aaattctcgg
     1141 atatgatgta ttcggattta catgatttct taccccctaa ttttgagatg ctgc
//
LOCUS       DAOSYE010000822         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111429_length_1192_cov_3.497801, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000822 DAOSYE010000000
VERSION     DAOSYE010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111429_length_1192_cov_3.497801"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PLU43_11845"
     CDS             complement(<1..115)
                     /locus_tag="PLU43_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOO29147.1"
                     /translation="MDEVINRLFEGDCLEYMKNIPAGSIDMILCDLPYGMTQ"
     gene            301..933
                     /locus_tag="PLU43_11850"
     CDS             301..933
                     /locus_tag="PLU43_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HOO29148.1"
                     /translation="MISREDMLELTRRMTIDRTSFTRIAGSYLDKDGLTDGTFNTNFL
                     KLKASERQKKLLVAKTIPFSNTNENLKDYHFSKDKIGNGSIWQLLQAMRLSGLKDDVL
                     LDTFYELIAEKYQTTKDYTIIVFHDRYDVPMKAADKERLGESEEVFEYIICAVCPLTG
                     EYEPGEPEFGFLFPLFTDRSADIHAIGLYNKNKEFPHRELEELLLQNEKS"
     gene            complement(963..>1192)
                     /locus_tag="PLU43_11855"
     CDS             complement(963..>1192)
                     /locus_tag="PLU43_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOO29149.1"
                     /translation="AEVDFVCKDETGIKYIQVAASVRDENTLARELSSLLKIADHYPK
                     YIMTLDKDPLADYDGIKRINALDYLLYKIEI"
BASE COUNT          396 a          239 c          206 g          351 t
ORIGIN      
        1 tttgtgtcat gccatacggc aagtcgcata aaatcatatc aatactgccg gcaggaatat
       61 ttttcatata ttccaggcag tctccctcaa acaatctatt tataacttca tccatatcta
      121 ttgtctcatc atttatcttc ttcatgatcg atctccttag taattatata actatattac
      181 catgcccttg tagcgaacac aagttctgtt tcagacatat cataaattat aatgaaaaat
      241 tctcataatg atgatacaat acgaatacaa catggaaaag aagaggaaaa cagacagcgc
      301 atgataagca gagaagatat gttagaactg accagacgca tgaccattga cagaacatcc
      361 tttacaagaa tcgcgggcag ttacctggat aaggacggct tgaccgacgg tacttttaat
      421 acaaactttt taaaattaaa ggccagtgaa aggcagaaaa agcttctggt cgccaaaaca
      481 attccatttt caaatacaaa tgaaaattta aaggactatc atttttccaa agacaaaatt
      541 ggaaacggaa gcatctggca gctgctgcag gcaatgcggc taagcggatt aaaagacgat
      601 gtactgctgg atacttttta tgaactgatc gctgaaaaat atcagacaac gaaagactat
      661 accattattg tatttcacga cagatatgat gttccaatga aagcagccga taaagaacgc
      721 cttggagaat cagaagaggt ttttgaatat atcatctgtg cagtctgtcc gctaactggt
      781 gaatatgaac cgggtgaacc ggagtttgga tttttattcc ctctgttcac cgacagaagc
      841 gctgacattc atgcaatcgg cctctacaat aagaataaag aatttccgca tagggagctg
      901 gaagaactgc tgctccaaaa tgaaaaatca taatatggcg atccttcact tctcacttat
      961 ttttatattt ctattttata tagcaggtaa tctaatgcat ttattcgctt aattccatca
     1021 taatcagcaa gcggatcttt atcaagtgtc atgatatatt tggggtaatg atccgcaatt
     1081 tttagcagcg aactcaattc ccttgccaat gtattctcat ctctgacact tgctgccacc
     1141 tgaatatatt ttataccagt ttcgtcttta catacaaaat ctacttccgc at
//
LOCUS       DAOSYE010000823         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111438_length_1192_cov_3.196130, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000823 DAOSYE010000000
VERSION     DAOSYE010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111438_length_1192_cov_3.196130"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1192)
                     /locus_tag="PLU43_11860"
     CDS             complement(<1..>1192)
                     /locus_tag="PLU43_11860"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOO29150.1"
                     /translation="EEFGNTLSVKDKKSLATLIYYPEEKLDIIMRGEENIEDWYKITL
                     HRLVQITKRVASKYTRSKVRKALPKDFAYVIEELITEKVEVQDKEAYYNEIIHTIIRI
                     GRAPDFIVALSNLIQRLVIDQLHIVGDVFDRGPGPHIVMETLCNYHSVDIQWGNHDVV
                     WMGAAAGHLACIANVLRMAAKYGSLDTLEEGYGINLIPLATFAMDVYRNADCDAFKIS
                     YNTDYDTKDLGLDTRMHKAMSIIQFKLEGQLMIRRPEFDMADRLLLDKIDYEKKTVMI
                     EGREYKLKDTDFPTIDPKNPYVLTNEEAQMMERIRHSFLKSEKLQKHVRFLFAKGSLY
                     KVYNSNLLYHGCVPLEEDGEFRKVNIFGKEYSGRPLYDILEHYARKGYYTTDDIEERM
                     KGQDI"
BASE COUNT          287 a          313 c          233 g          359 t
ORIGIN      
        1 aatatcctgc cccttcatgc gttcttcgat atcatccgtc gtatagtagc ctttccttgc
       61 ataatgctcc agaatatcgt ataacggcct gccgctgtat tcctttccga aaatattgac
      121 ctttctgaat tcgccgtcct cctcaagcgg tacacagccg tgataaagca gattggagtt
      181 gtatacttta taaagactgc cctttgcaaa taaaaaccgc acatgcttct gcagcttttc
      241 gctttttaaa aaggaatgcc tgatccgctc catcatctgt gcttcttcat ttgtcagcac
      301 ataggggttc ttcggatcga tcgtcggaaa atccgtatct ttcagtttgt attcccggcc
      361 ttctatcata accgtctttt tttcataatc gatcttatca agcagaagcc tgtcggccat
      421 atcaaattcc ggccggcgta tcataagctg tccttccagc ttaaactgta tgatcgacat
      481 tgccttatgc atcctcgtat caagtccgag atcctttgta tcgtaatccg tattatagga
      541 aatcttaaat gcatcgcagt cggcgtttct gtaaacatcc attgcaaacg ttgcaagcgg
      601 gatcagattg atcccgtagc cttcttccag cgtgtcaagg ctgccatact tcgccgccat
      661 acgcagaaca ttggcaatgc aggcaagatg gcctgctgcc gcacccatcc agacaacatc
      721 gtgatttccc cactgaatat caacagaatg ataattgcag agcgtctcca tgacgatatg
      781 aggacccggt cccctgtcaa atacatctcc gacaatatgc agctggtcga taacaaggcg
      841 ctggatcagg ttgctcagtg ccacaataaa atcaggtgcc cggccgatac ggatgatcgt
      901 atggatgatc tcgttataat aggcctcctt gtcctgtact tccacctttt cagtgatcag
      961 ctcttctatt acataagcaa aatccttcgg aagcgccttt cttactttgg aacgggtata
     1021 tttggatgcg actctttttg tgatctgtac gagacgatgc agtgtgatct tgtaccagtc
     1081 ctcaatattt tcttccccgc gcatgataat atccagcttt tcctccggat aatagatcag
     1141 cgtcgcaaga cttttcttgt cctttacgct cagtgtgttt ccaaactcct cg
//
LOCUS       DAOSYE010000824         1190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111774_length_1190_cov_2.518943, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000824 DAOSYE010000000
VERSION     DAOSYE010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111774_length_1190_cov_2.518943"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1190
                     /locus_tag="PLU43_11865"
     CDS             <1..>1190
                     /locus_tag="PLU43_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOO29151.1"
                     /translation="DEEDFDNTGMQSADTVVLLAKDTDAKTLTLRLVTGGKEQELSYD
                     GTTVILNRHGDSLSMAQLERGEILDIVYSTHSARLSSIRQSVNTWTNTGVTKFSIDEK
                     KKIMQIGDSQYQFTDDLVITSNGEPAQLMDLTQMDTLTVEGYNRKVCSIIVEKGHGYI
                     RLLNDVYFVGGWIEVGQSVIKPITAEMLLPVPEGVYTVRFTNGGYAGEEKVKIERDKE
                     KEIDLSKIEIEEVAVGHIAFQISPEYAQLYVDGEMTDYEERVSMEYGTHQLRVVAAGY
                     ETVETTIKVGSEFANIDIDLDKEEDTEEEDSDSDTGSKESSGSTAAGTSSSSTAAVST
                     TVSSSTAAATVSGDEKIYVEGPSGAEVYLDGNYVGIAPVSTSKVTGSHVITLSKSGYQ
                     TKSY"
BASE COUNT          343 a          232 c          344 g          271 t
ORIGIN      
        1 cggatgaaga ggattttgac aataccggta tgcagtctgc ggatacggtt gtgctgcttg
       61 caaaagatac ggatgctaag acgctgacgc ttcgccttgt tacaggagga aaggaacagg
      121 agctttctta tgacggaaca acggtaatct taaaccggca tggagacagc ctttccatgg
      181 cacagcttga aaggggagag atccttgata ttgtgtattc cacacacagt gcaaggctca
      241 gcagtatacg gcagtctgtg aacacatgga cgaatacggg agttacgaaa ttttccatag
      301 atgaaaaaaa gaagatcatg cagatcgggg acagccagta ccagtttacg gatgaccttg
      361 tgatcacttc aaacggggag cctgcacagc tgatggatct tacgcagatg gatacactga
      421 ctgtcgaggg ctataaccga aaggtctgtt ccatcattgt cgaaaaaggc catggatata
      481 tccgtctttt gaatgacgtt tactttgtcg gcggctggat cgaagtcgga cagtctgtta
      541 ttaagccgat cactgcggaa atgctgctgc cggtaccgga gggtgtttat acggtacggt
      601 ttacgaacgg cgggtatgca ggagaagaaa aagtaaagat cgaacgggac aaggaaaaag
      661 aaattgatct gtcaaaaatc gaaatcgaag aagtggcagt cgggcatatt gcatttcaga
      721 tttctccgga gtatgcacag ctttatgtag acggggagat gacggattat gaagaacgcg
      781 tatcgatgga gtatggaacg catcagcttc gtgtggtagc ggccggttat gaaacggtgg
      841 agactacgat aaaagtgggt tcggaatttg caaacatcga tattgatctg gataaggaag
      901 aggacaccga agaagaggat tccgattctg ataccggttc aaaagagtct tcaggcagca
      961 cggcggcggg gacttcttcc agcagcacgg cagcagtcag tacgacggtg tcttcgtcta
     1021 ctgcggcagc gacagtgtcg ggggatgaaa aaatatatgt ggaaggacct tccggtgcgg
     1081 aagtgtatct tgacggcaat tatgttggga tcgcgccggt cagcacttca aaggtgaccg
     1141 gttcccatgt gatcacccta agtaaaagcg gctatcagac aaaaagctat
//
LOCUS       DAOSYE010000825         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111877_length_1189_cov_3.510582, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000825 DAOSYE010000000
VERSION     DAOSYE010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111877_length_1189_cov_3.510582"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..171
                     /locus_tag="PLU43_11870"
     CDS             1..171
                     /locus_tag="PLU43_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO29152.1"
                     /translation="MAYVIGDSCISCGTCESQCPVSAISAGESKYEIDPETCIDCGSC
                     AGVCPVEAISAG"
     gene            complement(267..>1189)
                     /gene="dnaB"
                     /locus_tag="PLU43_11875"
     CDS             complement(267..>1189)
                     /gene="dnaB"
                     /locus_tag="PLU43_11875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOO29153.1"
                     /translation="EILEDTEKRIFDIVQQRNSGDYTPINEIVMNALEKINAASQMKG
                     NVTGIATGFNDLDYKTAGLQPSDLILIAARPSMGKTAFALNVAQHICFKLHMCAAIFS
                     LEMSKEQLVNRLLSMESHVDSQHIRTGNMKDNDWESIVEGAGVIGKSKLIIDDTPGIS
                     ISELRSKCRKYKLEHDLQIVIIDYLQLMSGSGRSDSRQQEISDISRALKSLARELRVP
                     VVALSQLSRAVEKREDHRPMLSDLRESGAIEQDADVVMFLYRDDYYNKETDKKGIAEV
                     IIAKQRNGPIGTVELVWMEDLTKFASITRQ"
BASE COUNT          302 a          299 c          234 g          354 t
ORIGIN      
        1 atggcatatg taattggtga ttcatgtatc agctgtggaa catgtgaatc tcagtgtcct
       61 gtcagcgcta tcagtgctgg tgaatcgaag tatgaaatcg atccggagac ctgtatagat
      121 tgtggttcct gcgcaggtgt ttgtcctgta gaggctatca gtgccggtta atcgtaaaac
      181 agcatgtgta tgtatacgca aaataaaaac cgccggacaa cttattgttc cggcgatttt
      241 tattttgtat cagactccct gacatcctac tgtcttgtta tactcgcaaa ttttgtaaga
      301 tcttccatcc atacgagttc taccgtaccg atcggaccat ttctctgctt ggcaataatc
      361 acttcggcaa ttcctttttt atcagtttct ttattataat aatcatccct gtataaaaac
      421 ataacaacgt cggcatcctg ttcaatagcc ccggattctc gcagatcgga cagcatcgga
      481 cgatgatcct ccctcttttc gaccgcacgc gacagctgcg acaatgctac aaccggtaca
      541 cgcagctctc ttgccaatga cttaagggct ctggaaatat cggatatttc ctgctgtctc
      601 gaatccgaac gcccgcttcc ggacatcagc tgcagatagt cgataataac gatctgtaaa
      661 tcatgctcca gcttatattt cctgcacttg gaacgcagct ccgatattga aattccaggc
      721 gtatcatcaa tgatcaattt agattttccg atcacaccgg ctccttccac aatactttcc
      781 cagtcattgt ctttcatatt gccggttctg atatgctgcg aatccacatg tgattccatg
      841 gaaagcagac gattgacaag ctgctccttc gacatctcaa gactgaatat tgccgcgcac
      901 atatgaagct tgaaacagat atgctgtgcc acattcaacg caaatgccgt tttccccata
      961 gatggtctgg ccgctatcaa tataagatca gaaggctgaa gaccggctgt cttataatcc
     1021 agatcattga agcccgttgc aattcccgta acattccctt tcatctgcga tgcggcattg
     1081 attttttcaa gtgcattcat gacgatttca ttgatcggcg tatagtcccc ggaattgcgc
     1141 tgctgcacga tatcaaaaat ccgtttctcg gtatcctcca gtatctcct
//
LOCUS       DAOSYE010000826         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_111909_length_1189_cov_2.702822, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000826 DAOSYE010000000
VERSION     DAOSYE010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111909_length_1189_cov_2.702822"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLU43_11880"
     CDS             complement(<1..595)
                     /locus_tag="PLU43_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOO29154.1"
                     /translation="MSAAKKYFFVMLAILIAQISVSAQNMHIHKKDHTIITIPMSEID
                     KITYSNTSSPSVQPSAVVPAAAGAWPKNTENTVIDAEGNTYKTVRIGRQTWMAEDLRS
                     TRFNTGATMPQIKDSDDWKKMSSPAYSWFDNYANTTGGYGVLYNGYAIENGYLCPSGW
                     HVPTVSDWDLLIRECGSMSVAGGRLKAKEKGFWDDPNA"
     gene            complement(691..1164)
                     /locus_tag="PLU43_11885"
     CDS             complement(691..1164)
                     /locus_tag="PLU43_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29155.1"
                     /translation="MRYIAFIIIFIMTVSCTINRTLSKYQSGEEFNSLAENKDAKVKL
                     VGGEVIPVKNISYDGEIFRFNLDSLTKGSLQVDSVANIDIYKAKRVMTALKYLGTGVV
                     VSILSGITAMFIADSDEKIWAEKTIALGFLCTAGFTVKGAVRGVEAHFKIVNWKK"
BASE COUNT          295 a          290 c          220 g          384 t
ORIGIN      
        1 ctgcattggg atcgtcccag aaaccttttt ctttcgcctt aagcctgcct ccggccacgc
       61 tcatgctgcc gcattctctg atgagaaggt cccagtcact cactgtaggc acatgccatc
      121 cgctaggaca gaggtatccg ttctctatag cataaccgtt atataacacc ccgtaaccac
      181 cggtcgtatt ggcataatta tcgaaccatg agtaagccgg cgaagacatt ttcttccagt
      241 cgtcggagtc cttgatctga ggcatggtcg ctccagtatt gaaccttgtg cttctcaagt
      301 cttcagccat ccatgtctgc cttccgatcc tgaccgtttt ataggtattc ccctcagcgt
      361 ctattaccgt attttcagtg tttttcggcc atgctcctgc tgctgcaggt actacggccg
      421 acggctgaac agagggcgat gacgtattcg aatatgttat tttatcaatc tcgctcatcg
      481 gaatagtaat tatagtgtga tcttttttgt ggatgtgcat gttctgggct gaaaccgaaa
      541 tctgcgcaat taagatcgcc agcattacaa aaaaatattt cttcgcagcg gacatcctgc
      601 ctccttactt tgttcttctt aatttacaaa attaatccgc aattgaccac aaaaattatt
      661 tacgcatgaa attcaatgtc cgttcttatt ctattttttc cagtttacga tcttaaaatg
      721 tgcttcaaca cctcttacag cgcctttgac cgtgaatcct gccgtacaaa gaaatccgag
      781 agcgatggtc ttttcagccc atatcttttc gtcactgtcc gcaataaaca ttgctgttat
      841 cccgctgaga atcgaaacaa ctactccagt ccctaaatat ttcagtgcag tcattacacg
      901 tttagcttta taaatatcta tattggctac actgtctact tgtaaacttc cttttgttaa
      961 gctgtctaaa ttgaatctga atatctctcc atcatagctg atattcttaa ccgggatcac
     1021 ttctccgccg accagtttta cttttgcgtc tttgttctca gccagagaat tgaattcttc
     1081 gccggactgg tattttgata aagtgcggtt tatcgtgcag gatactgtca taatgaaaat
     1141 aataatgaag gctatatagc gcataagagt cctccgatgt ttgatatat
//
LOCUS       DAOSYE010000827         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_112193_length_1187_cov_3.164311, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000827 DAOSYE010000000
VERSION     DAOSYE010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112193_length_1187_cov_3.164311"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1187
                     /locus_tag="PLU43_11890"
     CDS             104..>1187
                     /locus_tag="PLU43_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO29156.1"
                     /translation="MKFKKADLGVALYLLSAFVFLIIPIPSYLLDVCLAINIAVAFAI
                     LFNTMFAKEVLEMSFFPTVLLFTTIFRISLNVSSTRLILTTGAPGNVVETFGNFVGGG
                     DLIVGSIVFIILIVIQFMVINKGSERVAEVTARFTLDAMPGKQMAIDADLNTGAITDT
                     EAKAARDKIQQESSFFGSMDGAVKYVKGDATAGLIITLVNIVGGIIMGMTRNGLDISD
                     ALQKYAILTIGDGLVSQIPSLLISLSTGILVTKGSKQADFGNVLVKQLFGVPKVLYFV
                     GGTLCFLGVVTPLNAILFLVLGLVFILSGRFIANSLEVEKIEKEVDTEETAAAEIRKP
                     ENVVSLLQVDPIELEFGYGIIPLADVN"
BASE COUNT          299 a          223 c          310 g          355 t
ORIGIN      
        1 ggcacagatc ccgcctgagc tttatcaggc agttgccgaa atactggcgg tgatctacaa
       61 tgcgaaaaag agggcataat gaccaggaag ggaggaaggc ggaatgaaat tcaagaaagc
      121 cgatttaggc gttgcgttat acctgctgag tgcatttgtt tttctgatca tacccattcc
      181 ttcttatctt ttggatgtct gtcttgcgat caatatagcg gtggcatttg cgatcctgtt
      241 taatacgatg tttgcaaaag aagtactgga aatgtctttt tttccaactg ttctgctgtt
      301 cacaacgatc ttcaggattt cattgaatgt ttcttctaca agactgatac tgacaacagg
      361 agcacctggt aatgttgttg agacttttgg caattttgtc gggggaggcg acctgatcgt
      421 cggttccatt gtttttatta tattgattgt aattcagttc atggttatca ataaaggttc
      481 cgaacgtgtt gcggaggtaa cagccagatt tacgctggat gccatgcctg gtaaacagat
      541 ggcgatcgac gcggatctga atacaggtgc gatcacggat acggaggcaa aggctgcgcg
      601 tgataagatc cagcaggaat ccagcttttt cggttccatg gatggtgccg taaagtatgt
      661 aaaaggagat gcgacggcag gactgatcat tacactggtt aatatcgtag gcggtattat
      721 catgggaatg acaagaaacg gactggatat ttcggacgct ttacagaaat atgcgatcct
      781 gacgatcggt gacggtcttg tcagtcagat tccgtcactt ctgatctcat tatcgaccgg
      841 tattcttgtt acaaaaggct ctaagcaggc cgattttggg aacgtgcttg tcaaacagct
      901 gtttggcgtt ccgaaggtat tgtattttgt aggcggtacg ctttgtttcc ttggtgttgt
      961 tacaccgctg aatgcgattt tatttcttgt actcggcctt gtttttattt tatcaggacg
     1021 gtttattgca aattctcttg aggtggaaaa gatagagaaa gaagtagata cggaagagac
     1081 agcagcggcg gagatccgaa aaccggaaaa tgttgtttca ctcctgcagg tcgatccgat
     1141 cgagctggaa ttcgggtatg gtattattcc gcttgccgac gtgaatc
//
LOCUS       DAOSYE010000828         1186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_112354_length_1186_cov_3.030062, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000828 DAOSYE010000000
VERSION     DAOSYE010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112354_length_1186_cov_3.030062"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PLU43_11895"
     CDS             complement(<1..1022)
                     /locus_tag="PLU43_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO29157.1"
                     /translation="MLTVSLIIPIYNAETYLRRCLNSAMEQTYRNMEILLVNDGSTDS
                     SLEICKEYEKMDNRFRIINKENTGVSDTRNLAITFAKGKYLQFLDSDDWLTPDATETF
                     VYFAEKYDCDMVIADFYRVDGAVFTEKQHIRERGLLTREQFAEYMMEEPADFYYGVLW
                     NKLFKRNIIIHYTLKMDEALSWCEDFLFNLSYIRHAERFTAIQTPIYYYMKRKGSLVS
                     TTPKKGSIVKFKFQLLEYYKNLYQSMGLYEENKMRINSFVVAFAKDGNVAGRLSKKRK
                     KLDNGDYIEELPSGYTRTKHTFEPVFDENSRILILGSFPSVKSRENDFYYGNEQNRFW
                     ELIAKLT"
BASE COUNT          328 a          243 c          218 g          397 t
ORIGIN      
        1 gtcaattttg caatcaattc ccaaaaacga ttctgttcat ttccataata aaaatcattt
       61 tcccgtgatt ttacggaagg aaaacttccg agaatcagga tcctggaatt ttcatcaaat
      121 acaggttcaa atgtatgttt tgttcgtgtg tagccggaag gcagttcttc aatataatcc
      181 ccattgtcca gttttttgcg ttttttgctc aggcgtcctg caacatttcc gtcttttgcg
      241 aaggcaacaa caaaggagtt gatccgcatt ttgttctctt cgtacagtcc catactttga
      301 tacagatttt tatagtattc cagcagctgg aatttaaatt tgacgatgct tccttttttg
      361 ggcgtcgtac tgacaaggct gcctttccgc ttcatatagt aatagatcgg tgtctgaatg
      421 gcagtgaaac gttcggcatg gcggatatag gaaagattga acagaaaatc ttcgcaccag
      481 cttaatgctt catccatttt cagtgtataa tgaataatga tatttctttt aaaaagcttg
      541 ttccatagaa cgccgtaata gaagtcagcc ggttcttcca tcatgtattc cgcaaattgt
      601 tcacgtgtca ataggccgcg ttcacggata tgctgctttt ccgtaaaaac agcgccgtct
      661 acgcggtaaa agtccgcaat gaccatatca cagtcgtatt tttccgcaaa ataaacaaat
      721 gtttccgtag cgtccggtgt cagccagtcg tcactgtcca aaaactgcag atacttcccc
      781 tttgcaaatg taatagccag gtttcttgta tcggaaacgc cggtattttc cttgttgata
      841 attcgaaacc ggttgtccat tttttcatat tctttgcaga tctcaaggct gctgtctgtg
      901 cttccgtcat ttacaagcag tatttccata tttcgatagg tctgttccat tgcactgttc
      961 aggcagcggc gcagatacgt ttccgcatta taaattggaa tgatcaaact gacagtcagc
     1021 atctttttat ccttcttccc acaaataaat tgtatcaaac aaattctagt atattgtttt
     1081 tgcaaagtca agaagttgtg atgatctgtc atcataaaaa tatttgaaat gctgtttttc
     1141 tgttttataa ataaattgac aaaaaaccga tttatggata taataa
//
LOCUS       DAOSYE010000829         1185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_112510_length_1185_cov_3.289381, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000829 DAOSYE010000000
VERSION     DAOSYE010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112510_length_1185_cov_3.289381"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1185
                     /locus_tag="PLU43_11900"
     CDS             133..>1185
                     /locus_tag="PLU43_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO29158.1"
                     /translation="MKKEIKKGLATAAIFMILVAAVGCSGSKTTDETGSNGDTAVNTE
                     TEVDSGAAEDTGGSEDTGSQAEEEGSTSADGALVGFCANATSSSFVSWMAEATKELLE
                     ADGAEVQIADADMDTAAQISQIENFAVMGAKVIIIIPVDPDSLADSISYAQEKGSLVF
                     VLNSDTGVYDCLMTSDRYEIGAQTAQLTADWIDETFPDAADGSVKVAIFESRTNPEES
                     ADSDGMHEIEERCPKAKVVKTVDGISTNETAQEAISTLLQTNPEVQAVICYNSESCLG
                     ANEYVMSSGVVSDLSKFAVFGADYSDQVAQEIYLSTTDESVYRGTVKFGSDNIPQSIY
                     DIAIKMIKGEDFPKNQW"
BASE COUNT          369 a          225 c          318 g          273 t
ORIGIN      
        1 aaattttaaa cttctaaaga taaccatcca attcaaaaga ttgatgcatt gtacaggtat
       61 agtcaagatg ctagaattga acatggattt tgaagcatat gcagtcaaaa ttcataaaaa
      121 aggaggtgca gcatgaaaaa agaaataaaa aaaggattgg cgacggcagc aatttttatg
      181 attctggtcg cagcagtcgg atgttctggc agtaaaacaa ccgatgaaac aggcagcaat
      241 ggggatacgg cagtgaatac ggaaacagaa gtcgattcgg gggcggcaga agataccggc
      301 ggcagcgaag ataccggcag tcaggcagag gaagaaggtt ccacatcagc ggacggtgcc
      361 ctggtaggtt tctgcgccaa tgcgaccagt tcatcttttg tatcctggat ggctgaagca
      421 actaaggagt tgctggaagc agacggtgcg gaagttcaga ttgcagatgc ggatatggat
      481 acggcagctc agatcagcca aatagaaaat tttgctgtca tgggagcaaa ggtcatcatt
      541 ataataccgg tggatccgga cagtctggct gattctatca gctatgctca ggaaaaaggg
      601 tctctggtgt ttgtacttaa cagcgataca ggcgtttatg actgcctgat gacatctgac
      661 cgttatgaaa taggagcaca gacagcccag ctcacggcgg actggattga cgaaacgttt
      721 ccggatgcgg cagacggcag cgttaaggtg gctatttttg aaagccggac caatccggaa
      781 gaatctgcag actcggacgg tatgcatgaa attgaagaac ggtgcccgaa agccaaagtt
      841 gtaaaaactg ttgacggaat cagcaccaat gaaactgcac aggaagccat atctacattg
      901 cttcagacga atccggaagt gcaggcagtg atctgctaca attcagaaag ttgtctgggt
      961 gccaatgaat atgtgatgag ttcaggcgtt gtcagcgatc tttcaaaatt tgcagtgttt
     1021 ggtgcggact attcggatca ggtggctcag gaaatctatt tgtcgacaac cgatgaaagt
     1081 gtttaccgtg gtacggtaaa attcggcagc gacaatattc ctcagagtat ttatgacatt
     1141 gccataaaaa tgatcaaggg agaagatttc cccaaaaacc aatgg
//
LOCUS       DAOSYE010000830         1185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_112564_length_1185_cov_2.447788, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000830 DAOSYE010000000
VERSION     DAOSYE010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112564_length_1185_cov_2.447788"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLU43_11905"
     CDS             complement(<1..717)
                     /locus_tag="PLU43_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HOO29159.1"
                     /translation="MTIVHTDRTTWYKLDLSANVYPTLQRRNFSSVYRLSILLKEEII
                     PDLLQQAVDQTLPRFPSFKVALRKGLFWRYLEPNNRPGPFIEEDIINPCMPMDFKANH
                     RYLIRIYYYQNRISLEVHHSLADGSGGMYLLQTITAVYLRLCGHDITCEGYVLDIDQY
                     PDSSELEDAYVKYANSKVRPPRTQEKAYRVRGTKEKFYTLNIICGIMSTSQVLSAAKQ
                     KKASLTEYLNSVLLYALLEKQ"
     gene            858..>1185
                     /locus_tag="PLU43_11910"
     CDS             858..>1185
                     /locus_tag="PLU43_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO29160.1"
                     /translation="MAFLHIKFDKFGNQKIQQEDQDINKNNQKLMGVIKKMHSVVENE
                     DLDRQRHAQDNFGTLFGTHPFIEFRQQKMGMIDAEWTQLRHLENSSRVILYCHGGGYA
                     TGSCRYA"
BASE COUNT          343 a          234 c          295 g          313 t
ORIGIN      
        1 ttgtttttcc agcagcgcgt acagcagaac agaattcaga tattctgtaa gtgatgcctt
       61 cttttgtttg gcagcggaaa gaacctggct ggttgacata ataccgcata taatattcag
      121 tgtataaaat ttttcttttg ttccgcgcac acgatatgct ttttcctgcg tgcgcggcgg
      181 gcgtactttg gagttggcat atttgacata ggcatcttcc agctcggagg aatccggata
      241 ttgatcaata tcgagaacat agccttcaca ggtgatatcg tggccgcaca gccgcagata
      301 gaccgctgtg atcgtctgca gcagatacat gccgccgctg ccgtctgcga gcgaatgatg
      361 cacttccagt gagatccggt tttgatagta atagattcgg atcaggtagc ggtgattggc
      421 tttgaaatcc atcggcatgc acggattgat aatgtcttct tcaataaagg gacccggacg
      481 gttattgggc tctaagtatc tccagaacag gccttttctc aaggcaactt taaaggaagg
      541 aaaacgcggc agtgtctgat cgacagcctg ctgcagcaga tcgggaatga tttcttcctt
      601 taacaggata gaaagcctgt atacggatga aaaattacgc cgctgcagag taggataaac
      661 gtttgccgat agatccagct tataccatgt tgtgcggtct gtatggacga tcgtcataag
      721 catcctttca aaatagcaaa attatggata aacagagcct cctgtttgtt ttagtatact
      781 accggagtct gcttgtcaca acagttattt ttgtgttaaa ctgataaaag cattgttcga
      841 aaaaaaggag acaggacatg gcatttttgc atataaagtt tgataaattt ggaaatcaga
      901 aaatacagca ggaagatcag gatataaaca aaaataacca gaagctgatg ggcgtcatca
      961 aaaaaatgca cagtgttgtt gaaaatgagg atctggacag acagaggcat gcacaggaca
     1021 attttggaac gctgttcggc acccatcctt ttatagagtt tcggcagcaa aaaatgggaa
     1081 tgattgatgc ggagtggaca cagctaagac acctggaaaa cagcagcagg gtgatcttat
     1141 actgccatgg cggcggttat gcaacgggga gctgccgtta tgcac
//
LOCUS       DAOSYE010000831         1185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_112616_length_1185_cov_1.944248, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000831 DAOSYE010000000
VERSION     DAOSYE010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112616_length_1185_cov_1.944248"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /gene="rplS"
                     /locus_tag="PLU43_11915"
     CDS             complement(<1..271)
                     /gene="rplS"
                     /locus_tag="PLU43_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOO29161.1"
                     /translation="MNDIIKKIEDEQLKEQVSEFSVGDTVRVHAKVKEGNRERIQIFE
                     GTVLKKQGGSNRATFTVRKNSNGVGVEKTWPLHSPHVEKVEVVRRG"
     gene            complement(388..1065)
                     /gene="trmD"
                     /locus_tag="PLU43_11920"
     CDS             complement(388..1065)
                     /gene="trmD"
                     /locus_tag="PLU43_11920"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOO29162.1"
                     /translation="MNFHILTLFPDMILQGLHTSIIGRAAEKGLLSIEAVNIRDFAFN
                     KHHKVDDYPYGGGAGMLMQAEPVYLAYEAVRSKIEAAPRVVYLTPQGTVFTQAMAAEF
                     SKEEDLILLCGHYEGIDERVLEEIVTDYVSAGDYVLTGGELPAMMMVDAISRLVPGVL
                     NNDDSAESESFQNNLLEYPQYSRPEIWHGKKVPEVLLSGHHANIETWKQEQSVIRTKE
                     RRPDLLL"
BASE COUNT          296 a          324 c          204 g          361 t
ORIGIN      
        1 taccccttct tacgacttca accttttcaa catgcgggga atgcaacggc catgtttttt
       61 caacacctac accattggaa tttttcctta ctgtaaatgt cgccctgttg cttccgccct
      121 gttttttcaa aacagttcct tcaaaaatct gaattctttc acgatttcct tctttgactt
      181 tggcatgtac cctgacggtg tcgccgactg agaactctga aacctgttcc ttcagctgct
      241 catcttcgat ctttttaata atatcgttca tgtgtgacct ccttaatctt tggacgttct
      301 taatctcatt tcatcgaaca gaggaccgtc tctttttctc acaacgtatc atagtttagc
      361 atatatcccg cataaatgca agcaatttta taaaagcaga tccggcctgc gctcttttgt
      421 acggatcacg gactgctcct gtttccatgt ctcgatattg gcatgatgtc cggataacag
      481 cacttccgga actttttttc cgtgccagat ttccggcctt gaatattgcg gatattccag
      541 cagattattt tgaaaagact ctgattcggc cgaatcgtca ttattgagca caccgggtac
      601 aagccgtgaa atcgcatcga ccatcatcat ggccggcagt tctccgcccg ttaacacata
      661 atcaccggca gacacataat ccgtcacgat ctcctccaat accctttcgt caataccttc
      721 ataatgaccg caaagcagta tcagatcttc ttcttttgag aattccgctg ccattgcctg
      781 cgtaaaaacg gttccctgcg gagtcagata taccactctc ggcgctgctt ctattttgct
      841 tctgacagct tcataagcca ggtataccgg ctctgcctgc atcagcatcc ctgcgccgcc
      901 gccatacggg taatcatcca ctttatgatg cttgttaaaa gcaaaatccc ggatattaac
      961 tgcttctatc gacagaagtc ctttttcagc cgcacgtccg ataatacttg tatgcagtcc
     1021 ctgcaggatc atatccggaa ataatgttaa aatatgaaaa ttcattacaa aagtccctcc
     1081 agcaaatgca ccttcatgat catcctttca atatccacat gcaaaatgca ctgcctgatc
     1141 gcaggcagaa gcagctcttt tccgtcaggc atctcaatca tatat
//
LOCUS       DAOSYE010000832         1181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_113190_length_1181_cov_2.641208, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000832 DAOSYE010000000
VERSION     DAOSYE010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113190_length_1181_cov_2.641208"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLU43_11925"
     CDS             <1..564
                     /locus_tag="PLU43_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOO29163.1"
                     /translation="NKGKLDVAGAVGSGMLRVIKDMGLKDPYVGQVGLQTGEIAEDLT
                     YYFAVSEQVPSAVGLGVLMERDNTVRRAGGFIIQLLPGAEESLIGRLEEKLSGMPSVT
                     TLLDEGRTPQELLAYLLADFGVEILDTIPTKFYCNCDKKRVEKALISIGRKDLEEMIA
                     DGKPVEVKCHFCSRTYEFSIEELKDLI"
     gene            596..>1181
                     /locus_tag="PLU43_11930"
     CDS             596..>1181
                     /locus_tag="PLU43_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOO29164.1"
                     /translation="MRDGFIKTAAVTPRIRVADIEYNRGETEKLMKEATEGGAKVIVF
                     PELCLTGYTCQDLFLQPLLAEKAQEALCLLVKASEGTDALVFVGLPLEQDGKLYNTAA
                     VFQNGKLLAFIPKRYLPNYAEFYEMRHFSPGEADVIMIDFYGQQVPFGQNLLFCTEAV
                     KGLKIAAEICEDLWVPDPPSTSHAMNGANLIVNLS"
BASE COUNT          320 a          235 c          349 g          277 t
ORIGIN      
        1 aataaaggaa agcttgatgt ggcgggagct gtcggcagcg gtatgctgcg tgtgatcaaa
       61 gatatgggat taaaagatcc ttatgtggga caggtcggac tgcagacagg cgagatcgct
      121 gaggatctga cgtattactt tgcggtatct gaacaggtgc cgtcggccgt cggactgggc
      181 gtgctgatgg agagggataa tacggtgcgc agagcaggcg gatttatcat ccagctgctg
      241 ccgggtgcgg aagaatctct gatcggccgt ctggaagaga agctttcggg tatgccttcc
      301 gttacgacac tgcttgatga aggcaggacg ccgcaggagc tgcttgcgta tctgcttgca
      361 gacttcggcg ttgaaatact ggatacgatc ccaacgaaat tttactgcaa ttgtgataaa
      421 aaacgtgtgg agaaagcgct catcagtatt ggaagaaaag atctggaaga aatgatcgcg
      481 gacgggaaac cggtagaagt caaatgccat ttctgcagcc ggacatatga attcagtatt
      541 gaggaattga aagacctgat ataatgtaac agaaaagaac aggaaggcgg aaaatatgcg
      601 cgacggattt ataaaaacgg cagccgtgac accacgaatc agggttgcgg atatagaata
      661 taaccgcggt gaaacagaga aactgatgaa agaggcaaca gaaggcggcg caaaggtgat
      721 tgtctttccg gaactttgtc tgaccggcta tacatgccag gatctgtttc tgcagccgct
      781 tctggccgaa aaagcgcagg aagccctgtg cctgcttgta aaagcgtcgg agggcacaga
      841 tgcgcttgtc tttgtcggtc ttccgcttga gcaggacgga aagctttata atacggcagc
      901 cgtatttcag aacgggaagc tgctggcatt tattccgaaa agatatcttc cgaattatgc
      961 ggaattttat gaaatgagac acttttctcc gggagaagcc gatgttatca tgattgactt
     1021 ttacggtcag caggtgccgt tcggacagaa ccttctgttt tgtacggaag ctgtaaaggg
     1081 actgaagatc gcggcagaga tctgtgaaga tctgtgggtg cctgatccgc cgagtacttc
     1141 tcatgcgatg aacggggcta atctgatcgt caatctgtct g
//
LOCUS       DAOSYE010000833         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_113511_length_1179_cov_2.669039, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000833 DAOSYE010000000
VERSION     DAOSYE010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113511_length_1179_cov_2.669039"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLU43_11935"
     CDS             <1..836
                     /locus_tag="PLU43_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29165.1"
                     /translation="FLKSDEDMLQAFLYRQRRNPQDVHVINYLTAYEGFTLNDLVSYD
                     YKHNEENGEENRDGNNYNYSWNCGAEGSTRRKAVGKLRMRQMKNAFAMLLLSQGTPML
                     LSGDEFCNTQYGNNNAYCQDNKVTWLNWKQTKQSQELYEYVKELIALRKSHPILHMEE
                     ELRIMDYAACGYPDLSYHAEAAWYPKMENHIRHIGMMLCGMYARIDRLTKDSFFFIAY
                     NMHWESHKFGLPKLPKEMNWHYCLDTTDTLNRETVIKNLESKQDEITVPERSVLVLIG
                     Y"
     gene            920..>1179
                     /locus_tag="PLU43_11940"
     CDS             920..>1179
                     /locus_tag="PLU43_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO29166.1"
                     /translation="MLGDKRVLFSGMQATGTLTLGNYLGALKNWVTLSEEYECFYSVV
                     DLHSITIRQDPAELRKRARALLTLYIAAGLDPEKNCIYYQSHV"
BASE COUNT          377 a          218 c          293 g          291 t
ORIGIN      
        1 gtttcttaaa aagtgatgaa gatatgctgc aggcttttct ttacagacag cgcagaaatc
       61 cgcaggatgt tcatgtgatc aattatctga cggcatatga aggctttacc ctgaacgatc
      121 ttgtttccta tgattataag cataacgagg aaaatggaga agaaaacagg gacggcaata
      181 attataatta cagctggaat tgcggcgcag agggaagtac gagaagaaaa gcagtaggta
      241 agctgcggat gcgccagatg aaaaacgcat ttgccatgct gctgttgagt cagggtacgc
      301 cgatgctgct ttccggcgat gaattttgca atacccaata tggcaataac aatgcctatt
      361 gtcaggataa taaagtcaca tggctgaact ggaaacagac aaagcagtcg caggaactgt
      421 atgagtatgt aaaagaattg atcgcattaa gaaaatcaca tccgatcctg catatggaag
      481 aagaactgcg gatcatggac tatgcggcgt gcgggtatcc ggatctgtcc tatcatgcgg
      541 aagcagcctg gtatccgaag atggaaaacc atatcaggca tatcggtatg atgttatgcg
      601 ggatgtatgc aaggatcgac agactgacaa aagacagttt tttctttatt gcatataata
      661 tgcattggga atcacataaa ttcggacttc ccaaacttcc caaggagatg aactggcatt
      721 attgtctgga tacgaccgat actcttaaca gggaaaccgt cataaaaaat ctggaatcaa
      781 aacaggatga aattacagtg ccggaaagat cggtgcttgt gctgatcgga tactgagtaa
      841 aattcgaacg ctggcaattg acgcagattg ccggcagtga taaactagaa aaatgaaaca
      901 gaagctggag gatatcaaca tgttaggtga caaaagggta ctgttttccg ggatgcaggc
      961 aaccggaaca ctgacacttg gtaattattt aggagccctg aaaaactggg tgacattaag
     1021 tgaggaatat gaatgttttt attccgtagt ggatctgcat tccattacga tcagacagga
     1081 ccctgcggaa ctgcgaaagc gtgcgcgcgc actgcttact ttatatatag cggccggcct
     1141 tgatccggaa aaaaactgta tttattatca gtctcatgt
//
LOCUS       DAOSYE010000834         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_113535_length_1179_cov_2.419929, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000834 DAOSYE010000000
VERSION     DAOSYE010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113535_length_1179_cov_2.419929"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="hisG"
                     /locus_tag="PLU43_11945"
     CDS             complement(<1..339)
                     /gene="hisG"
                     /locus_tag="PLU43_11945"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOO29167.1"
                     /translation="MRYLTFALGKGRLADQTLALLEKTGITCEEMKDKTTRKLIFVNE
                     ELKLKFFLAKGPDVPTYVEYGAADIGVVGKDTIMEENRKSYEVLDLGFGKCRMCICGP
                     ASARDLLLHHE"
     gene            complement(371..>1179)
                     /locus_tag="PLU43_11950"
     CDS             complement(371..>1179)
                     /locus_tag="PLU43_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOO29168.1"
                     /translation="ELINDGSPEADAEMVSLIIACMKQAGLHDFQVSIGNVEFFKGLC
                     MEYGIDEDAEMELREMISNKNYFGAQEILDSLSLSDEIKETILKITEMFGPVETIREA
                     KKIVTNKRSLLAVSRLEAIHEILEKENTAEYVTFDLGMLSKYHYYTGVIFRAYTYGTG
                     DAVIKGGRYDSLLREFGKKTEAIGFAVVVDHLLSALSRQEIPVETKEETFLMLYKQKD
                     RDKAYKKAKEYREKGKKTILMRMDQDEHAADYGQYAEMNHFHIILVEHLT"
BASE COUNT          286 a          293 c          221 g          379 t
ORIGIN      
        1 ttcatggtgc agcagcagat ccctggccga agccggtccg catatgcaca tccggcattt
       61 tccgaagcca agatccagca cttcataact cttgcggttt tcctccatga tcgtatcttt
      121 gccgacaacc ccaatatcgg cagccccgta ctccacatag gtaggtacat ccggtccttt
      181 tgccagaaaa aatttcagtt ttagttcctc gttgacaaaa atcagtttcc ttgtagtttt
      241 atctttcatt tcttcacagg taattcctgt cttttcaagc agtgcaagcg tctggtctgc
      301 aagtctgcct tttccaagtg caaatgttaa atatctcata gcatcctctt ttatttttca
      361 atgttttaaa tcatgtcagg tgttcaacta aaataatatg aaaatgattc atttccgcat
      421 actggccgta atctgcagca tgctcgtcct gatccatacg catcagaatt gtttttttcc
      481 ctttttctct gtattctttt gctttcttgt aagctttatc cctgtctttt tgtttataaa
      541 gcatcaaaaa agtctcttct tttgtttcaa caggaatctc ctgccgagac agtgccgaaa
      601 ggagatggtc tacaacgact gcaaatccga ttgcctcggt ttttttgccg aattcacgga
      661 gcagactgtc atagcggccg cctttgatga ccgcatcgcc ggttccatat gtataagctc
      721 ggaaaataac accggtataa taatggtatt tgctgagcat tccaagatcg aaggtcacat
      781 attccgccgt attttctttt tcgagtattt catggatcgc ctccagccgc gaaacggcaa
      841 gcagggagcg tttgtttgtt acgatctttt tggcttcccg gatcgtttcc accggaccga
      901 acatttccgt gatcttcaaa atggtttctt ttatttcatc cgacagagac agcgaatcca
      961 gaatttcctg agcaccaaaa taatttttgt tggaaatcat ttcacgcagt tccatttccg
     1021 catcttcatc gattccgtat tccatgcaga ggcccttgaa aaattcaaca ttgccgatac
     1081 tgacctgaaa atcgtgcaga ccggcctgtt tcatgcaggc aatgataaga gaaaccattt
     1141 ccgcatccgc ttcaggactc ccgtcgttga tcagttctg
//
LOCUS       DAOSYE010000835         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_114131_length_1175_cov_3.718750, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000835 DAOSYE010000000
VERSION     DAOSYE010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114131_length_1175_cov_3.718750"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLU43_11955"
     CDS             complement(<1..424)
                     /locus_tag="PLU43_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29169.1"
                     /translation="MKEKEQGFVKKLLNSRNTGLLLGFAVLLIVAGILTPSMFSVNSI
                     LNMLQNNAVYALLAAGMMVVIITGGIDLSVASTLSLAGVVCSKLMSEHMDVPAIVWVL
                     LGIAVGAACGAFNGFIIGYLKMVPMIATLGTMYIYRGFA"
     gene            complement(421..>1175)
                     /locus_tag="PLU43_11960"
     CDS             complement(421..>1175)
                     /locus_tag="PLU43_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29170.1"
                     /translation="LIGQPVLQVEHLSKEGYFRDISFDVRKGEILALTGLVGAGRTEV
                     CQTIFGIMTPDFGSVELEGKRVHIKSPVDALELGIGLLPEDRQLQGLINELPIYQNVS
                     SAVMKKFTKNHMLDVEKEEANAIALCQKIQLKAKDISAPPSSLSGGNQQKVVFAKLLS
                     CDLKVLILDEPTKGIDVGAKYSIYEIMNDLTKRGYAIIMVSSEMPEVLGMADRIVVMK
                     SGRVTGNFNSKDVTQEMILEASLRQHGEETEA"
BASE COUNT          301 a          340 c          228 g          306 t
ORIGIN      
        1 atgcaaaccc tctgtaaata tacatcgtac caagcgttgc gatcatcggc accattttca
       61 ggtatccaat gataaatccg ttgaatgcac cacaggccgc tcctaccgcg atccccaaaa
      121 gcacccagac aattgccggc acatccatat gctcactcat cagcttggag cagacaacac
      181 ctgccaggct cagtgtggag gcaacagaca gatcgatgcc gcccgtaatg ataacgacca
      241 tcatgccggc tgccaaaagt gcatataccg cattgttctg cagcatgttc aaaatggaat
      301 taacggaaaa catggacggc gtcagaatgc cggcgacgat cagaagcacc gcaaatccaa
      361 gcaataagcc cgtatttctg ctgtttaata atttttttac gaaaccctgt tccttttctt
      421 tcatgcttcc gtctcctctc cgtgctgtct taacgaagct tccagtatca tttcctgcgt
      481 cacatctttg gaattaaaat taccggtcac ccgtccggat ttcattacta cgatcctgtc
      541 cgccatacct aatacctccg gcatttcaga agacaccata atgatcgcat acccccgctt
      601 tgtcagatca ttcatgatct catagatcga atatttggca ccgacatcaa tgccttttgt
      661 cggttcatcc agaatcaata ctttcagatc acagctaagc agtttcgcaa aaacgacctt
      721 ctgctgattg ccgcctgata aagaagacgg cggcgcgctg atgtcctttg ctttcagctg
      781 tattttctgg cagagcgcaa tggcattggc ctcctccttt tcaacgtcaa gcatatgatt
      841 ctttgtaaac tttttcatga cagccgaaga cacgttctga tagatcggaa gctcattgat
      901 cagcccctgc agctgcctgt cttccggcag cagcccgatc ccaagctcca gcgcgtcaac
      961 cggagatttg atatgcaccc gtttcccctc cagttcaaca cttccaaaat ccggtgtcat
     1021 aatgccgaag atcgtctggc agacttctgt tctgcctgct ccgacaagac ctgtcagtgc
     1081 aaggatctct cctttcctga catcaaagga aatatccctg aaataacctt cctttgacag
     1141 atgttctacc tgcaggaccg gctgcccgat caatg
//
LOCUS       DAOSYE010000836         1172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_114677_length_1172_cov_2.560430, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000836 DAOSYE010000000
VERSION     DAOSYE010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114677_length_1172_cov_2.560430"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>1172)
                     /locus_tag="PLU43_11965"
     CDS             complement(133..>1172)
                     /locus_tag="PLU43_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO29171.1"
                     /translation="TYMANMAKERMEDESDAEEGMAATEELENSDEEPEEAQILDTDA
                     DVSEESEGEGTPMGEESAVASPEVLQRLSTMIPSTIDDILTFANNYDAPEEVEAVFKW
                     DVSDIFYNYFFIGNYVSVGGENGDDNTIFNIYNQQAVDCLTVYQDMNQFFSIDADEVT
                     YDSILKEFIEGKTVFTVATTDAVAKIEEAKANGEFEYDYGVAVLPDVSSTLKARGLSV
                     TTTIAVNGYSEKKEQANSFAEYLAYTKADDLYKKSGKLACRKSVNYDNSEIDNIMAEY
                     EKSVSLPKMIEASNYWVQLEIAFTKVWTGSSPDETLKELSDTIGAQIDEIEYHIPSQE
                     TIGAGAEEDLR"
BASE COUNT          296 a          316 c          189 g          371 t
ORIGIN      
        1 ctttatctgc ttttttagtg ttgaacatat catacaaata aactatgaaa taaatttgta
       61 agtcacgtga gaaaaaagtg aaaactatac aataaatctg tgattaatct gtgtccaaac
      121 tgtgaactca tcctatctta aatcttcctc tgctcccgcg ccgatcgttt cctgcgaagg
      181 aatatgatat tcgatctcat cgatctgcgc accgatcgta tccgataatt ctttcaaagt
      241 ctcatccgga ctgctgccgg tccacacttt tgtgaaggcg atctcaagct gtacccagta
      301 attgcttgcc tctatcattt tcggcagcga tacggatttt tcgtactctg ccataatatt
      361 gtcgatctca gaattatcat aatttacaga tttcctgcat gccagtttcc cggatttttt
      421 atacagatca tccgcctttg tataagcgag gtattccgca aatgaatttg cctgttcctt
      481 tttttccgaa tatccgttga cagctatcgt cgttgttacg gaaagaccgc gggccttcag
      541 cgtcgaactg acatccggca gcactgcaac cccgtaatca tattcaaatt ctccatttgc
      601 ctttgcttcc tcgatctttg cgaccgcatc cgttgtcgcg acggtgaaca ctgtctttcc
      661 ctcaataaat tccttcaata tactgtcata agtcacttca tccgcatcga tcgaaaaaaa
      721 ctgattcatg tcctggtaaa cagtcaggca atccactgcc tgctgattat aaatattaaa
      781 gatcgtatta tcatcgccgt tttcaccgcc gacactcaca taatttccaa taaaaaaata
      841 attataaaaa atatccgata catcccattt aaatactgcc tcaacttcct ccggggcatc
      901 ataattattt gcaaacgtca gaatatcatc tatcgtcgaa ggaatcatgg ttgacagccg
      961 ctgcagtact tccggactcg caacagcgct ctcctctccc atcggtgttc cttccccttc
     1021 gctttcttcg gaaacatccg catccgtgtc taatatctga gcttcttccg gctcttcgtc
     1081 cgaattttcc agctcttccg ttgccgccat tccttcctcg gcatccgatt catcctccat
     1141 cctctcttta gccatattgg ccatatacgt ct
//
LOCUS       DAOSYE010000837         1170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_114934_length_1170_cov_3.480717, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000837 DAOSYE010000000
VERSION     DAOSYE010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114934_length_1170_cov_3.480717"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1170)
                     /locus_tag="PLU43_11970"
     CDS             complement(59..>1170)
                     /locus_tag="PLU43_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29172.1"
                     /translation="ALVHNGAFLLVDAINPDGTIVPELYHGMIKDIYGITKQYEPYVS
                     GDIRHNAAVWFASHAKYDPDETGADMNSKSFGRKIYMDAPVAATSILRANNIPYDVIG
                     SKNIVKETADVMILSHVANIREEEMDALEAYLGKGGNLYISGPIGNQRLQELLGIKIT
                     GKTEHTFTYMSPTEEGAAYFEGFSKNVPLTVPMAQYEATLETQEDCTVLATLTLPYTM
                     TGTEQFSAIHSNPPGIYTKKPCVIRKTIGKAVVIWCAAPIELSKPYMSRQVFCGMIRS
                     LGGELKFESNAPKFVEILNWKKDGREYFAVINQQEESPVAPVYDIWIKVGGNKRAVMA
                     DNSYELHTETEEKKTVIRLPKLEIFQIFEVKEEQL"
BASE COUNT          267 a          318 c          235 g          350 t
ORIGIN      
        1 tactttttgt aatcggctgc ccattccttt tcccaatagg caaaatagat tccatatttc
       61 acagctgttc ctcctttact tcgaaaatct gaaaaatctc cagctttgga agacggatca
      121 ccgttttctt ttcctccgtc tccgtatgca gttcataact gttgtcggcc atcaccgccc
      181 gtttgttccc gccgactttg atccagatat catagaccgg tgcgaccgga gattcttcct
      241 gctgattgat gaccgcaaaa tattctctcc cgtccttttt ccagttcagt atctcgacga
      301 atttcggcgc attggattca aatttcagtt ccccgccaag cgaccgaatc atcccgcaaa
      361 agacctgtct gctcatataa ggctttgaca gctcgatcgg tgcggcacac catatgacaa
      421 cggcttttcc gatcgttttt ctgataacac acggtttttt cgtataaata ccgggcggat
      481 tggaatgaat ggcagaaaac tgctctgttc cggtcattgt atacggcagc gtaagtgtcg
      541 ctaaaaccgt acagtcttcc tgtgtttcaa gcgtcgcctc atactgagcc atcggcacag
      601 tcagcggcac atttttcgaa aatccttcaa aataagccgc cccttcttca gtcggactca
      661 tataggtaaa cgtgtgctct gttttgcctg ttatctttat tccaagcagc tcctgaagcc
      721 tttgatttcc gatcggaccg ctgatataca gattcccgcc ttttccaaga taagcttcca
      781 gcgcatccat ctcttcttct ctgatattgg ccacgtggga caggatcatg acatccgctg
      841 tttctttcac aatatttttg cttccaatca cgtcgtaggg aatgttattg gcacgcagaa
      901 tacttgttgc ggcaaccggc gcgtccatat agatctttct gccaaagctc ttggaattca
      961 tgtcggcacc ggtttcatcc ggatcatatt ttgcgtgact tgcaaaccat acggccgcat
     1021 tatgcctgat gtcaccgctg acatacggtt catactgctt cgtaatgcca tagatgtcct
     1081 tgatcattcc atgatacagc tcaggtacga tcgttccgtc cggattgatc gcatccacaa
     1141 gcagaaaggc cccgttatga acaagcgctg
//
LOCUS       DAOSYE010000838         1170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_114947_length_1170_cov_3.082511, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000838 DAOSYE010000000
VERSION     DAOSYE010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114947_length_1170_cov_3.082511"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLU43_11975"
     CDS             complement(<1..681)
                     /locus_tag="PLU43_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOO29173.1"
                     /translation="MSTDRFRINFKKYGLLFLVLLTAVLSAGCKKQEEQTPVINIEEE
                     ETTDAVSENETEASAVLEIINPVAADVAEMDDVNDPQAEETTDGENDGAKVEITALKN
                     EFETSGVTLGIDVAKWQGTIDWQQVAATGIEFAIIRVGYRAEATGVIYEDPCAKYNLQ
                     QAQAAGLKLGVYFYSTAVTEEEAAEEAAWVTSFIAQYPITYPVAYNCENFKAETSRQY
                     SLTKEERSS"
     gene            834..>1170
                     /locus_tag="PLU43_11980"
     CDS             834..>1170
                     /locus_tag="PLU43_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29174.1"
                     /translation="MVHEIYPKTFCPAYADKTPVLSDYSLAYLDNYILVSYKNGQVSL
                     PKFSDIKSFYQKDITFLFAQSYYLFAVDDLPYFLISLPEDLKSALPVRESLPQEQAAA
                     LFFFDLYKFR"
BASE COUNT          287 a          294 c          201 g          388 t
ORIGIN      
        1 agaacttcgt tcttcttttg tcagagaata ctgacggctt gtttcagcct taaaattctc
       61 acagttatac gcaaccggat aggtgatcgg atactgcgca ataaagcttg tgacccatgc
      121 cgcctcttcg gccgcttctt cctcagtgac tgccgtagaa taaaaatata cgccaagctt
      181 cagtcccgcc gcctgtgcct gctgtaaatt gtattttgca cagggatctt catagatcac
      241 accggtcgct tcggcccgat atccaacacg aatgatcgca aattcgatcc ctgttgcagc
      301 aacctgctgc cagtcgatcg ttccctgcca tttggccaca tcaataccaa gcgtcacacc
      361 gcttgtttca aattcatttt ttaatgccgt gatctctacc tttgcaccat cgttttcccc
      421 gtcggtcgtt tcctctgcct gcggatcatt tacatcgtcc atttccgcaa catctgctgc
      481 caccggattt ataatttcaa gcacagcgct tgcttcggtc tcattttcag atacggcatc
      541 tgtcgtttcc tcttcctcta tattaatgac cggcgtctgt tcttcctgct tcttacatcc
      601 tgcagacaat actgctgtca acaagactaa aaacaacagt ccgtattttt taaaatttat
      661 cctgaacctg tccgtactca tatttttacc cttcccaaga gaatcttttt ttattcagta
      721 taacagtcag agtatacctc ttttgactgc aaaaatccat agctggtatt tgctttattt
      781 ttattttcag cgtatgatgt aaaaaacaaa ctttaaaagg caggatctta tttatggtgc
      841 atgaaattta cccaaaaacc ttttgtcccg catatgcaga caaaactccc gtattatcgg
      901 attattcatt agcatatctg gacaattata ttcttgtgag ctataaaaac ggacaggttt
      961 ctcttccaaa gttttctgat atcaagtctt tttatcaaaa agacattacg tttttattcg
     1021 cacagtccta ttacctgttt gcagtcgacg atcttccgta ttttttgatt tctttaccgg
     1081 aggatttgaa aagcgcgttg cctgttcggg aatctcttcc gcaggagcag gcagcagcac
     1141 tgttcttctt tgatctttac aaattccggg
//
LOCUS       DAOSYE010000839         1170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_114956_length_1170_cov_2.979372, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000839 DAOSYE010000000
VERSION     DAOSYE010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114956_length_1170_cov_2.979372"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLU43_11985"
     CDS             complement(<1..660)
                     /locus_tag="PLU43_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29175.1"
                     /translation="MDKARIMEGVKKYTMIIVLVLVTAFFAWRTGGKILLPQNISNLI
                     SQNAYVFVLATGMLLCILTGGNIDLSVGSVVCFIGAVGGTLMENKGVNVYAAIIAMVL
                     TGILIGIWQGFWIAFIKIPPFITTLSGMLVFRGLSNVVLQGMTVSLKSETFIKIFGGG
                     ANCYFPDLFNNEGFNITCFVVGVIICLAYIAIQLKDRITKAKKGYQVETLSSMVIKMV
                     LI"
     gene            complement(664..>1170)
                     /locus_tag="PLU43_11990"
     CDS             complement(664..>1170)
                     /locus_tag="PLU43_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29176.1"
                     /translation="YVTEDRKGNGLILSNPIKINTTLANLGAVSKHMIIDKDKEYAVA
                     VEYKDMLKTKCPTVEQNVGNLSGGNQQKVLLAKWMFADPEILILDEPTRGIDVGAKYE
                     IYCIINDLVANGKSVIMISSELPEVLGMCDRIYVMNEGKIVGELSGEEASQEIIMSHI
                     VKTSNKGA"
BASE COUNT          353 a          287 c          188 g          342 t
ORIGIN      
        1 gatcagcacc atttttataa ccatggaaga gagtgtttct acctgatacc cttttttggc
       61 ttttgtgatc ctgtccttta actgaatggc aatataagcc agacaaatga taacaccaac
      121 aacaaaacag gtaatattaa atccttcatt gttgaaaaga tccgggaaat agcagtttgc
      181 gccgccgccg aaaattttaa taaatgtctc cgatttcagc gaaacggtca taccctgcag
      241 cacaacattc gataatcctc tgaacaccag cataccggaa agagttgtaa taaacggagg
      301 aatctttata aatgcgatcc aaaagccctg ccatataccg atcagaatac cggtcaaaac
      361 cattgcaata atggctgcat aaacatttac acctttattt tccattaatg ttccgccgac
      421 tgctccaata aaacatacga ctgagccgac cgaaagatcg atattaccac cagttaaaat
      481 acacagcagc ataccggttg caagcacgaa cacatatgca ttctgcgaga tcagattact
      541 gatattttga ggcagcagga tttttccgcc tgtccgccat gcaaaaaatg cagtgaccaa
      601 aaccaaaact ataatcattg tatatttttt aacaccttcc attattcttg ccttatccat
      661 agtctacgct cctttattac ttgttttaac aatatgcgac ataataattt cctgggaagc
      721 ctcttctccg gacagctctc caacgatctt tccttcattc atgacataaa ttctgtcaca
      781 catgccaaga acctccggca gctctgaaga aatcataatg accgatttcc cgttcgccac
      841 gagatcgtta ataatacaat aaatctcata tttagcgccg acatcgatac ctcttgtcgg
      901 ttcgtccaaa atcaaaattt ccggatccgc aaacatccat tttgcaagaa gtactttctg
      961 ctgattccca ccgcttaaat ttccgacgtt ctgctcaaca gtcggacatt tggttttcag
     1021 catatcctta tattcaactg cgacagcata ctccttgtct ttatcaatga tcatatgttt
     1081 actgactgca ccaagattgg caagtgttgt gttgattttg atcggattcg acagaatcag
     1141 gccgtttcct tttctgtcct ccgttacata
//
LOCUS       DAOSYE010000840         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_115116_length_1169_cov_2.719031, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000840 DAOSYE010000000
VERSION     DAOSYE010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115116_length_1169_cov_2.719031"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1169
                     /locus_tag="PLU43_11995"
     CDS             150..>1169
                     /locus_tag="PLU43_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29177.1"
                     /translation="MKRRVLVIALSAAMALSLTACGQQDSWADTESTESTESTESTAA
                     ADTAEPSEDTAQAAEEDSDSSNTLVIAMGSGFSTLDPGYMYEQNPILVANACYETLFK
                     FENGNDEPQLSLATGYEFSEDGKDCTITLNPDAVFASGNPVTSADVAFSLNRTKNLQG
                     NPAFICDNIDSVECPDDNTVIIHQETADSALISKLCYAACSVLDSEVVKANGGTDAED
                     ASTADSAQTYLDSASAGSGMYILKSYTPDEEIVLEKNPNYYGESTNVDKYIIKLEDNA
                     NTQMMGLSSGDIDIALNLNDDTMEELAENSDVVTENTSSKTVGFIMMNEDPTIGGPVS
                     DPDVQQ"
BASE COUNT          379 a          213 c          306 g          271 t
ORIGIN      
        1 gcgaactttg tgcaagggag ataccggagt ggaaagaaat tcaaccggat cattttgttg
       61 cctgtcatct ttataacgat aacggcaaaa tgagataaaa aagcgaatta gaacagaatg
      121 ttcattcgat aaaaaaagga ggagaaacta tgaaaagaag agttttagta attgcattgt
      181 cggcagctat ggctttgtcg ctgacggcat gcggacaaca ggacagctgg gcagatacgg
      241 aaagtacaga aagtacggag agtacggaaa gtactgctgc ggcagacacc gcagaaccat
      301 ctgaagacac ggcacaggca gcagaagagg acagtgacag cagtaatacg cttgtgatcg
      361 caatgggatc cggattttcg acactggacc cgggatatat gtatgaacag aatccgatac
      421 tggtggcaaa tgcatgttat gaaacgctgt ttaaatttga aaatggcaat gatgaaccgc
      481 agctgagtct tgcgacagga tatgaatttt ccgaggacgg taaagactgt acgatcacat
      541 tgaatccgga tgcggtattt gcaagcggca atcctgtcac ctctgcggat gttgcatttt
      601 cgctgaacag aacaaaaaat ctgcagggaa atccggcatt tatctgtgac aatattgaca
      661 gtgtggaatg tcctgacgac aatacggtca ttatccatca ggagacagcg gacagtgcgc
      721 tgatttccaa gctgtgctat gcagcctgca gtgttttaga cagtgaagtc gtaaaagcaa
      781 acggaggaac tgatgctgag gacgcatcta cggcagacag tgcacagaca tatctggatt
      841 cggccagtgc aggttcaggg atgtatattc tgaaaagcta tacaccggat gaagagatcg
      901 ttcttgagaa gaatccaaat tattatggcg agagtacaaa tgtcgataaa tatatcatca
      961 aactggaaga caatgcaaat acacagatga tgggtctttc aagcggagat attgatattg
     1021 cactgaatct gaatgatgat acgatggaag aacttgcgga aaacagtgat gttgtgacgg
     1081 aaaacacctc ttccaagacg gtaggcttta ttatgatgaa tgaagatcct acaataggcg
     1141 gtcctgtttc agatccggat gtacagcag
//
LOCUS       DAOSYE010000841         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_115236_length_1168_cov_3.141959, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000841 DAOSYE010000000
VERSION     DAOSYE010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115236_length_1168_cov_3.141959"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLU43_12000"
     CDS             <1..568
                     /locus_tag="PLU43_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO29178.1"
                     /translation="FDEIEKAHPEVFNVLLQVLDDGRITDSQGRTVDFKNTILIMTSN
                     IGSEYLLEGIDRDGSIKPETEALVMNELRVHFRPEFLNRLDETILFKPLTKSNIGGII
                     KLLINDLNRRLSDRNLSIRLTAAAEEHIVENGYDPLYGARPLKRYVQKYVETLAAKLI
                     LSDAVKEGDMIVIDVQDGELAAAAETVE"
     gene            616..>1168
                     /locus_tag="PLU43_12005"
     CDS             616..>1168
                     /locus_tag="PLU43_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29179.1"
                     /translation="MRKQYDRRLYVPVIGLIVLGMSGCLYGCREKPDILSQEPVIPLI
                     QREEEHNQEEYKQEDAGAAASSSLPLPETAGEDAANTVSSDMAGPAAVSSDTSTANIV
                     STQLSEEELESFSDYFNETRVNGFLSCSYEIPSWIDISAVLRSIGETGETVTAAEYHE
                     IKESLGSPESANTVKYSLDYIDTL"
BASE COUNT          330 a          226 c          331 g          281 t
ORIGIN      
        1 gttcgatgag atcgaaaagg cacacccgga agtgttcaat gtactgctgc aggtgcttga
       61 tgacggcagg atcacagatt cacagggcag gacggtggat tttaaaaata cgatactgat
      121 catgacttcc aatatcggat cggaatatct gctggaagga attgaccggg atggatcgat
      181 caagccggag acggaagcgc ttgttatgaa tgaactgcgt gtacatttca gaccggagtt
      241 tttgaaccgt ctggatgaga cgatcctatt caagcctttg acaaagagta atatcggcgg
      301 tatcattaaa ctgctgatca acgatctgaa caggagactt tccgacagga atttaagcat
      361 caggctgaca gccgccgcag aggaacatat tgtggaaaac ggatacgatc cgctgtatgg
      421 ggcaagaccg ctgaaacggt atgtgcagaa gtatgttgag acacttgcgg cgaagctgat
      481 attgtccgat gcggtcaaag aaggagatat gatcgtgatt gatgtgcagg acggtgaact
      541 ggctgctgct gcggaaactg tcgaatagtt tgttttaaga tttacagggg acagaggttg
      601 caatcaaaag gaaaaatgag aaaacaatat gaccgccggc tgtatgtgcc ggtaattgga
      661 ctgatcgttc tggggatgtc cggctgttta tatggctgca gggaaaaacc ggacatcctt
      721 tcacaggaac cggtcattcc gctgatccaa agagaggaag aacataatca ggaagaatat
      781 aaacaggaag atgcgggggc tgccgcatct tcctccttgc cgctgccgga aactgcagga
      841 gaggacgctg ccaatacggt gtcgtctgat atggccggtc cggcggccgt gtcgtcggat
      901 acatcgacag caaatatcgt cagtacacag ctgtccgaag aagaattgga gagtttttcg
      961 gattatttta atgaaacccg tgtcaatggt tttctttcat gcagttatga gattccgtca
     1021 tggatagata tcagtgcggt tttacggagt atcggtgaga ccggtgaaac ggtcacggcg
     1081 gctgagtatc atgaaataaa agagagtctt ggctcgccgg aaagcgcgaa tacagtcaaa
     1141 tattcgcttg attatattga tacgcttt
//
LOCUS       DAOSYE010000842         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_115445_length_1167_cov_2.199640, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000842 DAOSYE010000000
VERSION     DAOSYE010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115445_length_1167_cov_2.199640"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLU43_12010"
     CDS             complement(<1..1126)
                     /locus_tag="PLU43_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HOO29180.1"
                     /translation="MCKALLSCRKKIWEVLHDINLSLISLAPAEFIHFGTTHELLDLV
                     DRQIGDYEFLDWKRAVSTNAGQVSYAVNNSLIMDSLIGEGCYMENACVKNAVVGEGSI
                     LSQIEAEGITVPAHVVMHGLKLSDGNFLVRIYGVSDNPKETLLNDVDFLGTKLSVMCE
                     RYQLREVLWDKQETAYLWTARLYPSAAGWKEACADALLIYRMAALKASDQEVDAWKNR
                     ERYSLETGYNAADVTDIVIWNHELENRILVEKYLTAIDSGTDAYTAIRIFGKVGITGK
                     RLALLLDKAKEADFSTKIRIYYNLSLYLSQTERDFDDLTAELLEEKCFAEIRNTIYEN
                     AMKKIIYSAGREIRKDSVSVQLPVRVNWGGGWTDTPPHCVE"
BASE COUNT          293 a          332 c          215 g          327 t
ORIGIN      
        1 gctcgacaca gtgcggaggc gtgtccgtcc agcctcctcc ccagttcaca cgaacaggca
       61 gctgcactga tacagaatct tttctgatct cccttccagc ggaataaata atttttttca
      121 tggcattttc gtagatcgta ttcctgatct ccgcaaagca tttttcctct aaaagctctg
      181 ctgtcagatc atcaaaatcc cgttctgtct gagaaagata aagagacaga ttataataga
      241 tccttatttt tgtggaaaaa tccgcttcct ttgccttatc aagcagtaat gccagacgtt
      301 ttccggtaat gccgaccttc ccaaagatcc gtattgccgt atacgcatcc gttccactgt
      361 ctattgcggt caaatatttt tccacaagga tcctgttttc cagctcgtga ttccatatga
      421 cgatgtcagt cacatccgca gcattataac cggtttccag actgtaacgc tctctgttct
      481 tccatgcatc cacttcctga tcagatgctt tcagcgcggc cattcgatag atcagcagtg
      541 catccgcaca cgcttccttc cagccagcag ccgatggata cagtctggcc gtccacagat
      601 acgcagtctc ctgtttgtcc caaagaacct cacgcagctg atagcgctca cacatcacag
      661 acagctttgt accaagaaaa tccacatcat tcagcagtgt ttcctttgga ttgtccgata
      721 cgccgtaaat ccgcaccaga aaattaccat cggacagctt cagaccatgc atgactacat
      781 gcgccggcac tgtgattcct tctgcctcta tctgcgataa aatactgccc tctcctacaa
      841 cggcattttt tacgcaggca ttttccatat agcagccttc ccctataagg gaatccatga
      901 tcagactgtt atttaccgca taggacacct gtccggcatt tgtactgaca gcacgtttcc
      961 agtcaagaaa ttcataatcc ccgatctgcc tgtccaccag atcaagcagc tcgtgtgttg
     1021 tcccaaaatg aataaattcc gccggcgcca gactgatcag agataaattg atgtcatgca
     1081 gtacttccca tattttttta cggcaggaaa gaagcgcctt acacatcttt ccttccggag
     1141 cttcctcata atattcttcc agcgttg
//
LOCUS       DAOSYE010000843         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_115750_length_1165_cov_2.579279, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000843 DAOSYE010000000
VERSION     DAOSYE010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115750_length_1165_cov_2.579279"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLU43_12015"
     CDS             complement(<1..942)
                     /locus_tag="PLU43_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29181.1"
                     /translation="MNRHKRKAAIDLALLLTLSPAMNIYVSAASAAGISKTQEEETVT
                     TGTQEAAKEKEADKKQEASENDEYADDEDTQEAAAEPVEWEDIQINSTADLVALSRSC
                     WLDTWSKNKRVCLNCDLKLANSEFVSIPTFGGYFDGQGHTIEGLCVDDAVSYTGLFCY
                     VQENAVITDLTVKGTVQPSGEQTAVGGIAGDNSGTIQNCKFDGIVEGSDYTGGIAGFN
                     ELTGSLVACTCSGYVTGTHYTGGIVGENMGNIMRCINTAEVNSSNVDKGMSLDDINLE
                     QYTSNLFNLDAAGNDTDASEVVNSTVDTGGIPGQSIGV"
     gene            complement(929..>1165)
                     /locus_tag="PLU43_12020"
     CDS             complement(929..>1165)
                     /locus_tag="PLU43_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29182.1"
                     /translation="VYYEYADPYKDRIRTFRIDGVVRGRINGVVTGTMHAIVRGNVSA
                     YVEAGDLTEITEDSAGTADISDEIPEEVLAHEQA"
BASE COUNT          257 a          363 c          222 g          323 t
ORIGIN      
        1 cacgccgatc gactgtccgg gaattcctcc cgtatccacc gtactgttga caacctccga
       61 tgcatcggta tcattgccgg ccgcatcgag attaaacaga ttcgaagtat actgctctaa
      121 gttgatatcg tccaaagaca tgcccttatc aacgttggaa ctgttcactt ccgccgtatt
      181 gatgcaccgc atgatattgc ccatattttc tccgacgatc cctcccgtat aatgcgttcc
      241 tgtcacatat ccggaacatg tacacgccac aagactgccc gtcagttcat taaacccggc
      301 aattccgccc gtataatcac ttccctcaac gattccgtca aacttgcagt tttggatcgt
      361 tccgctgttg tctccggcga tcccgcctac tgcggtctgc tcgccggacg gctgaaccgt
      421 tcccttcact gtcagatccg taatgaccgc attttcctgt acataacaga acagccccgt
      481 ataggacacc gcatcatcca cacagagacc ttcaatcgta tgcccctgcc cgtcaaaata
      541 cccgccgaac gtcggtatcg atacaaattc actgtttgca agcttcagat cgcagttcag
      601 acagacccgt ttgttcttcg accacgtatc aagccagcag cttctggaaa gtgcgacaag
      661 atccgcagtg gaattgatct gaatatcttc ccattcgacc ggttctgccg ctgcttcctg
      721 tgtatcttca tcatccgcat attcatcatt ttcggatgct tcctgctttt tatccgcttc
      781 tttttccttt gccgcttcct gcgtccctgt cgtaacagtc tcctcctcct gcgtcttaga
      841 tatgcctgca gccgacgcag ccgatacata aatattcatt gccggagaca atgttaacag
      901 caatgccaga tctattgcgg cttttctctt atgcctgttc atgcgctaaa acctcctcag
      961 gtatttcgtc tgatatatct gccgtcccgg cagaatcttc ggtaatctcc gtcaaatcgc
     1021 ccgcttccac ataagcgctg acatttcccc gtacgatcgc atgcatcgta ccggttacaa
     1081 cgccgttgat cctgcccctg acaactccgt ctatccgaaa agtcctgatg cggtccttgt
     1141 acggatcggc atattcataa taaac
//
LOCUS       DAOSYE010000844         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_116260_length_1162_cov_2.803071, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000844 DAOSYE010000000
VERSION     DAOSYE010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116260_length_1162_cov_2.803071"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1162
                     /locus_tag="PLU43_12025"
     CDS             <1..>1162
                     /locus_tag="PLU43_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO29183.1"
                     /translation="GLASPEKIREWSRGEVLKPETINYRTLKPEKDGLFCERIFGPSK
                     DWECHCGKYKKIRYKGVICDRCGVEVTKANVRRERMGHIELAAPVSHIWYFKGIPSRM
                     GLILDLSPRTLEKVLYFANYIVLDPADTDLKYKQVLSEREYQEYRMNWGSKFRVGMGA
                     EAVKELLHAIDLEEEADELKTALKESTGQKRAKIIKRLEVVEAFRGSGNKPEWMIMDV
                     IPVIPPDLRPMVQLDGGRFATSDLNDLYRRIINRNNRLKRLLDLGAPDIIVRNEKRML
                     QEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKSGRFRQNLLGKRVDYSGRSVIVV
                     GPELKIYQCGLPKEMAIELFKPFVMKELVANGTSHNIKNAKKSTFKTVTPFDF"
BASE COUNT          346 a          209 c          321 g          286 t
ORIGIN      
        1 cgggctggca tcaccggaaa agattagaga atggtccagg ggagaagtat taaaacctga
       61 gacgatcaat tatagaacgc taaaaccgga aaaagacggt ctgttttgtg aaagaatctt
      121 tggaccgagc aaggactggg aatgtcattg cggtaagtat aaaaaaatcc gttacaaagg
      181 tgttatttgt gatcgctgtg gtgttgaagt tacaaaggca aatgtccgca gagaacgtat
      241 ggggcatatt gagcttgcag cacccgtatc acatatttgg tattttaaag ggataccgag
      301 caggatgggt ctgattttgg atctttctcc gaggacactg gaaaaagttc tttattttgc
      361 aaattatatt gtacttgacc cggcagatac ggacctgaag tataagcagg tgctgtctga
      421 acgggaatac caggaataca ggatgaactg gggaagcaag tttcgtgttg gaatgggtgc
      481 ggaagctgtt aaggagcttt tgcatgcaat cgatctggaa gaagaggctg atgagctgaa
      541 aacagccctg aaagagtcta ccggtcaaaa gagagccaag atcattaaaa ggctggaagt
      601 ggtagaagca ttcagaggct cgggaaataa gccggaatgg atgatcatgg atgtgatccc
      661 ggtcattccg cctgatctgc ggccaatggt tcagctggac ggcggcagat ttgcgacgtc
      721 tgatctgaac gatctctata gacgtattat taacagaaac aatcgtttga aaaggctgct
      781 ggatcttggt gcgcctgata ttattgtcag aaatgaaaaa agaatgctgc aggaagcagt
      841 agatgccctg atcgataacg gcagacgcgg aaggccggtt acaggaccgg gcaaccgggc
      901 actgaagtcg ctgtcggata tgttaaaggg taaatccgga cgtttccgtc agaacctgct
      961 tggcaagcgt gtcgattatt cgggacgttc cgttattgtt gttggtccgg aactgaagat
     1021 ctatcagtgc ggactgccaa aagaaatggc aattgagctg tttaagccgt ttgttatgaa
     1081 agagcttgtt gcaaacggaa catcgcataa tattaaaaac gcaaagaaat caactttcaa
     1141 gactgtcact ccctttgatt tc
//
LOCUS       DAOSYE010000845         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_116302_length_1162_cov_2.280939, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000845 DAOSYE010000000
VERSION     DAOSYE010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116302_length_1162_cov_2.280939"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>1162)
                     /locus_tag="PLU43_12030"
     CDS             complement(70..>1162)
                     /locus_tag="PLU43_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOO29184.1"
                     /translation="QQAEPVLLQTEEFSSDIIMDGRKMILRHTIKIAASDRKLWTPDT
                     PYLYYYSVRCGADEVAGYFALRTFSVEPDENGVARMCLNHRPYFQKGVLDQGYWPDGL
                     YTAPSDSAMLYDIMKMKELGFSMLRKHLKIEPQRWYYHCDRLGMIVWQDMVNGGGRYR
                     FWFVTYLATAFNMFKYRIRDNCHHLLARSDRRGREQFEQEMERTVRQLFGHPSIGTWV
                     IFNEGWGQFDAVRMTEKMRALDSSRVIDSTSGWFDQGCGDLRSQHYYFFKLVLQWEKK
                     RATVISELGGFPYRIEEHSFSDKVYGYHACNTQEELHAAYRHLMQDIIEPEIQKGLSA
                     TVYTQLSDVEEEVNGILTYDREVCKHADL"
BASE COUNT          275 a          344 c          235 g          308 t
ORIGIN      
        1 tcaacacatg caaccatgcc cgccagatca tccctgcctt cttctgcaat caccagttta
       61 ttctgttcct tataaatctg catgtttaca tacctccctg tcataagtca aaattccgtt
      121 tacttcctct tccacatctg aaagctgtgt atagactgtc gcggacagcc ctttttgtat
      181 ttccggttca ataatgtcct gcatcagatg cctgtacgcg gcatgcagtt cctcctgcgt
      241 attgcacgca tgatacccgt atactttatc gctgaagctg tgctcttcga tccggtatgg
      301 aaatccgccc agctccgaaa tgaccgttgc ccgcttcttt tcccactgca gaacgagttt
      361 aaaaaagtaa tagtgctggc tcctaagatc cccgcagccc tgatcaaacc agccgcttgt
      421 agaatcgatc acacggctgc tgtcaagcgc ccgcatcttt tctgtcatac gtaccgcatc
      481 aaactggccc cagccttcat taaagatcac ccacgtaccg atggacggat gtccaaaaag
      541 ctgccgcacg gtccgctcca tttcctgttc aaactgctct ctgccgcgcc tgtcgcttct
      601 tgccagcaga tgatggcagt tgtccctgat ccggtatttg aacatattga atgcagtagc
      661 cagataggtg acgaaccaga aacgataacg tccaccgccg tttaccatgt cctgccagac
      721 gatcatcccc aatctgtcac agtgataata ccagcgctgc ggctcgatct tcagatgctt
      781 tctgagcatg gaaaatccca gttccttcat cttcataata tcataaagca tggcagaatc
      841 actcggtgcc gtataaagac cgtccggcca gtatccctga tccagcacgc ccttttgaaa
      901 atatggcctg tggttcaggc acatgcgtgc cacgccgttt tcatccggtt caacagagaa
      961 tgtgcgaagc gcaaaatagc cggccacctc atcggcaccg caccgtacac tgtaatagta
     1021 caggtacggc gtatccggtg tccaaagctt cctgtcggaa gctgcaattt ttatcgtatg
     1081 gcgcaggatc atttttcttc cgtccataat aatatcactg gaaaactctt ctgtttgcag
     1141 caatacaggc tccgcctgct gc
//
LOCUS       DAOSYE010000846         1158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_117036_length_1158_cov_1.532185, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000846 DAOSYE010000000
VERSION     DAOSYE010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117036_length_1158_cov_1.532185"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLU43_12035"
     CDS             <1..288
                     /locus_tag="PLU43_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29185.1"
                     /translation="IRDNTHISCIFPELPDNVCPLFLPVYVRDNRDNFRSHLTENRIY
                     APVHWPKPAGVDLSDCKGAKYIMENIISIPVDQRYSLADMDRISYIIKSFR"
     gene            481..>1158
                     /locus_tag="PLU43_12040"
     CDS             481..>1158
                     /locus_tag="PLU43_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOO29186.1"
                     /translation="MLRDIADFKNLKGKIEKGKYFDISSAYGYGGPLYETDNLSELKH
                     EFFEIFTKYCADNNIITQFDRFHPLLDNHHFFEGYSELYKIRKTVHIDLKDEDTIWSG
                     MDPKCRNMIRKAEKNLVEIVLDDEKETLSEFTRLYQGTMQRNGTSDYYFFSDKFFSDT
                     ISNLGENIMIANARFDNRIIASALFMKSENYLHYHFSGSDPEYRNLQANSLLLYKAAL
                     YGLENGIK"
BASE COUNT          394 a          197 c          218 g          349 t
ORIGIN      
        1 atcagggata atacacatat ttcatgtatt tttcctgaac tgcctgacaa tgtctgcccg
       61 ttgttccttc ctgtatatgt cagggataat agagacaatt tcagaagtca ccttacagaa
      121 aacagaattt atgcacctgt gcattggcca aaacctgctg gagtcgatct ttcagactgt
      181 aagggcgcaa aatacattat ggaaaatatc atttcaatac ctgtagatca aagatacagc
      241 cttgcagata tggatagaat ttcatatatt atcaagtctt ttagataaaa aaaaacggag
      301 attgatctga accacaaaat tgttaaagat aaggtcgaat gggatttaat tattgaaagc
      361 tttaataata atgatgtgta tttcagatat ggttattttg tccctttcgc tagctacggc
      421 gacggagaac caaatctttt ttattacgaa tctgaagctg gaaaagttgc ctatcctttt
      481 atgcttagag atatagctga ttttaaaaat ctgaaaggta aaattgaaaa aggaaagtat
      541 tttgacatca gctccgccta cggatacggc ggccctctct atgaaactga taatctttcg
      601 gaactaaaac atgaattctt tgaaatattt acaaaatact gcgccgataa taatataatt
      661 acacagttcg accggtttca tcctctctta gataatcatc atttctttga aggatattca
      721 gagctgtata agatcagaaa gaccgttcac attgatctga aagatgagga cacgatctgg
      781 agcggcatgg atcctaaatg caggaacatg ataaggaaag cggagaaaaa tcttgttgaa
      841 attgttctgg atgatgaaaa agaaacgctt agcgaattca ccagactgta tcagggtact
      901 atgcaaagga acggaacatc ggattactat tttttctctg ataaattttt cagtgacaca
      961 atatcaaatc tgggtgaaaa tattatgatc gccaatgccc gttttgataa caggataatt
     1021 gcatctgcct tatttatgaa gagtgaaaat tacttacatt atcatttttc cggttccgat
     1081 ccagagtaca gaaatctgca ggcaaacagc cttttattgt acaaagcagc tttatacggc
     1141 cttgaaaatg gaataaaa
//
LOCUS       DAOSYE010000847         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_117090_length_1157_cov_3.315789, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000847 DAOSYE010000000
VERSION     DAOSYE010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117090_length_1157_cov_3.315789"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..962
                     /locus_tag="PLU43_12045"
     CDS             15..962
                     /locus_tag="PLU43_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOO29187.1"
                     /translation="MIDLMLMNGLLNAVKPGTRLIMVGDSDQLPSVGAGNVLGDILES
                     EFIYSVKLNEIFRQAAESLIVVNAHRINRGEYPDCNTKDKDFFMIRKKSEREVLASIL
                     ELCGNRLPSYYKDLDPSRDIQVLTPVRKGLLGCVNLNKEIQSIMNPSNELLSEKVYGE
                     KIFRENDKVMQIKNNYMLKWKMADDFAEGEGVFNGDVGTIKKIDNEFNELTVEYESNK
                     YVVYDFSQLDELEPAYAVTVHKSQGSEFPVVVIPVSWFPPVLSTRNLLYTAVTRAKKA
                     VIVVGSEIRMQAMVDNNSIKERYSGLKARLQGFLDRGSI"
BASE COUNT          422 a          184 c          259 g          292 t
ORIGIN      
        1 tagatgagag ctccatgata gatctgatgc ttatgaacgg acttctaaat gccgtaaaac
       61 ccggtacgag actgataatg gtgggagatt cggatcaact gccttctgtg ggagccggaa
      121 atgtgcttgg agatatactg gaaagtgaat ttatatattc agtaaaatta aacgagatat
      181 tcagacaggc ggcagaaagc cttatagttg taaacgcaca caggataaac agaggagaat
      241 atcccgattg caatacaaaa gacaaagatt tttttatgat cagaaaaaaa agtgaaagag
      301 aagtattagc ttctatattg gaactttgcg gaaacagact gccctcatac tataaggatc
      361 tggatccatc gagagacata caggtattga cgcctgtgag gaaagggctt ctgggctgcg
      421 tcaatctcaa taaagagatc cagagcataa tgaatccctc caacgaatta ctttcagaaa
      481 aagtatacgg agaaaaaata tttcgtgaaa atgacaaagt gatgcagata aaaaacaatt
      541 atatgcttaa atggaagatg gcggatgatt ttgccgaagg agaaggagtt tttaacggcg
      601 atgtgggaac aataaaaaaa atagacaatg aattcaatga acttacagta gaatatgaaa
      661 gtaacaagta tgttgtttat gatttttccc agttggatga actggagcct gcttatgccg
      721 tcactgtaca taaaagccag ggcagtgaat tccctgtggt cgtgattccc gtatcatggt
      781 tcccgcctgt gctttctacc agaaatcttc tttataccgc tgtaacaaga gcaaaaaaag
      841 ctgttatagt agtaggatct gaaataagaa tgcaggcaat ggtggataat aacagcatca
      901 aagagcgata ttccggactc aaggcaagac ttcaaggatt cctggacaga ggaagcatat
      961 aaaaagagaa aaaggcgaaa gtatgaaaat aaaagaaaat atattcgaag ttatatttcc
     1021 ttctaatata tactgtatat gctgcggaag tattatagac aaaagcaggc cttatgcttt
     1081 gtgtgattcc tgtatcaaaa aatttcactg ggccatcagc aaaacatgca aaaaatgtgg
     1141 taagatcctg gaagaaa
//
LOCUS       DAOSYE010000848         1156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_117258_length_1156_cov_2.683924, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000848 DAOSYE010000000
VERSION     DAOSYE010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117258_length_1156_cov_2.683924"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PLU43_12050"
     CDS             complement(<1..116)
                     /locus_tag="PLU43_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO29188.1"
                     /translation="MNFTYIIKCSDHSLYTGWTNNLEKRIASHNAGTASKYTR"
     gene            249..902
                     /locus_tag="PLU43_12055"
     CDS             249..902
                     /locus_tag="PLU43_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOO29189.1"
                     /translation="MLKKLREPGSAITHFIGVLFSAAVATPLIMRAAQTSVRNAAAMT
                     IFAFSMLLLYSASTTYHSIRFSAAVIKVFRKIDHMMIFILIAGSYTPVCLIVLKGTVG
                     TAMLCAVWGIAAAGILFKALWITCPKWVSSVLYIGMGWTCVFAFVPLWNILSAAAFAW
                     LLAGGVFYTVGGVIYACKLSLLNSRHANFGSHEIFHLFVLAGSFCHFIFMYVFVANM"
     gene            complement(907..>1156)
                     /locus_tag="PLU43_12060"
     CDS             complement(907..>1156)
                     /locus_tag="PLU43_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29190.1"
                     /translation="REFKDNRMQKDTVVCNRSQDTRTHKVFSALETVCYEFDLSKPIW
                     LDSTINEFQRHAKVRFTQDNFIDAVDFDYLEIQIIEED"
BASE COUNT          283 a          231 c          269 g          373 t
ORIGIN      
        1 cgtgtatatt tggatgccgt tccggcgtta tgggatgcga tccgcttttc caggttattt
       61 gtccagcctg tgtacagaga atgatcactg cattttataa tataggtaaa gttcatgtga
      121 aaccccttcc aaatttatta aatttatata aactgcttgc atattttttt cacatgaagt
      181 agtataatat caacaacaca tagtattgaa aactacatca tgaagagctg gcgaaaggag
      241 aacgtaccat gttaaaaaaa ttaagagaac caggcagtgc aatcactcat tttatcggag
      301 tattgttttc ggctgccgtg gcgacgccgt taattatgcg ggcagcacag acaagtgtgc
      361 ggaatgcggc cgctatgacg atttttgcat ttagtatgct tttgctgtat tcagcaagca
      421 caacctatca ttccatcagg ttttcggcgg ctgttatcaa ggtattcaga aaaatcgacc
      481 atatgatgat atttatattg attgcaggtt cctatacgcc ggtctgcctg atcgtactga
      541 aaggaacggt tgggaccgca atgctctgtg ccgtatgggg aattgccgca gcaggtattt
      601 tgtttaaggc cttatggatc acctgcccga aatgggtatc ctccgtactg tatattggca
      661 tggggtggac ttgtgtattt gcgtttgtcc cgctgtggaa tattctttcg gctgcagctt
      721 ttgcatggct tttagccggc ggtgtttttt atacggtcgg cggcgtgatc tatgcctgca
      781 aactttcttt gctgaacagc agacacgcaa atttcggttc ccatgagatc ttccatctgt
      841 ttgttttggc agggagtttc tgtcatttta tctttatgta tgtatttgtg gcaaatatgt
      901 agaacgtcag tcttcttcaa tgatctgaat ttccagatag tcaaaatcta cagcgtcaat
      961 gaaattgtcc tgcgtaaagc ggacctttgc atggcgctga aattcgttta tcgtggaatc
     1021 aagccagatc ggcttgctta ggtcaaattc ataacaaact gtttccagtg cggaaaaaac
     1081 cttgtgggtc cgcgtatcct gcgatcggtt gcatacgacg gtatctttct gcatgcggtt
     1141 gtctttaaat tccctg
//
LOCUS       DAOSYE010000849         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_117498_length_1155_cov_2.027273, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000849 DAOSYE010000000
VERSION     DAOSYE010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117498_length_1155_cov_2.027273"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PLU43_12065"
     CDS             complement(<1..>1155)
                     /locus_tag="PLU43_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29191.1"
                     /translation="GITMYYYDSVINSIEEELYIPYTRSYYMLKQDIDCLSYVNTRDV
                     FYLMLNGTVYNINLESKKVETVVTGLDESRFVASGDNSVIAWQTGTELSQYQTIELFA
                     LNALVPTEITADAGSIIIPLGFMNQDFIYGIARTGDISKDASGSTIVPMYELRIQSVT
                     GEVLMEYEQNGLYILDTDITDNMISLNRAYKNEETGEYIEASQDQIMNNQSEEETENI
                     YTSVVTDEMETTYQTVLAKEPSSDTMKLLTPKEVIFEGSRAITLDEEDTVERYYVYVM
                     GRIAGVYTDPAKAVNAAEEVFGVVVDKNDDYIWESGSRKEKTQLENIEEVQADEEHTS
                     AEICLDAMMKYDGVYENIPALSGSDTVLSMLENNMDATVLDMTGCSLSAVL"
BASE COUNT          313 a          303 c          187 g          352 t
ORIGIN      
        1 agcacagcac ttaaggaaca gcctgtcata tcaagtacag tggcatccat attgttttca
       61 agcatggaca gcaccgtatc gctgccggac agggccggta tattttcgta aacaccatca
      121 tatttcatca ttgcatcaag acagatctca gcagatgtat gttcttcgtc agcctgtacc
      181 tcctcaatat tctcaagctg cgttttttct ttcctgctgc cgctttccca tatataatca
      241 tcgtttttat caacaacaac gccaaatact tcttccgccg catttaccgc ttttgccgga
      301 tccgtataga ctcctgcgat cctgcccatc acataaacat aatagcgctc gaccgtatct
      361 tcctcatcta aagtgatcgc acggcttcct tcaaaaataa cttctttagg agtcagcagc
      421 ttcattgtat cggaagaagg ttcttttgca agcactgtct gataggtcgt ttccatttca
      481 tcggtaacaa cacttgtata aatattctct gtctcctctt ctgactgatt attcatgatc
      541 tggtcctgcg aagcctcgat atattcccct gtttcctcgt ttttgtaagc acggtttaat
      601 gagatcatat tatccgtaat atccgtatcc agaatataaa gcccattctg ctcatattcc
      661 atcagcactt ctcccgtaac actttgaata cggagctcat acatcggaac gatcgtactt
      721 cccgacgcgt ccttagaaat atctcctgtc ctcgcaattc cataaataaa gtcctgattc
      781 ataaacccaa gcggaataat aatactgccc gcatcggccg taatttccgt aggcacgagc
      841 gcatttagtg cgaacagttc gatcgtctga tactgactca gctccgtacc tgtctgccag
      901 gcgatcacac tgttatctcc tgatgcgaca aaccggcttt catccagtcc ggttacaact
      961 gtttccacct tcttggattc cagattaata ttatagactg taccatttaa catcagataa
     1021 aaaacatccc gcgtatttac ataggataaa caatcaatat cctgcttcag catataataa
     1081 gatctcgtat acggaatata cagctcctct tctatagaat taataacgct gtcataataa
     1141 tacattgtaa taccg
//
LOCUS       DAOSYE010000850         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_117747_length_1153_cov_3.108379, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000850 DAOSYE010000000
VERSION     DAOSYE010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117747_length_1153_cov_3.108379"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..>1153)
                     /locus_tag="PLU43_12070"
     CDS             complement(25..>1153)
                     /locus_tag="PLU43_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOO29192.1"
                     /translation="MAALMTSVIDNPGKVAEYIYTCKTMGIAILPPDINEGMAGFSVS
                     GNSIRYGLASIKSIGWPVIEAIVEERKEHGKFKDLKDFALRLSGREVNKRTVESFIKS
                     GAFDSLGGTRKQFMNVYTQIMDTVSQDKKNNLAGQLSLFDIVGEDDRETFEVGLPDVG
                     EFGKELLLSYEKEVLGIYVSGHPLEADEAMWKKHITAYTSDFVLDAETGITKVQDGRK
                     ATIGGIIEHKTIKHTKNGRAMAFITLEDLVGTVEVIIWPGDYEKNVKWLTEDSKVFIK
                     GRVSAEEEKDAKLICESIIPFANMPKKLWIKFLTRDDYQKQEAKLLDVLNDFAGPDAV
                     AVYIEETKEIKKFSGNQTVFAGEALVAKLSGLFGTENVKIV"
BASE COUNT          282 a          332 c          199 g          340 t
ORIGIN      
        1 aaaaaataat acagccgata tccgttacac aatttttaca ttctccgtcc caaacagtcc
       61 ggaaagtttt gcaaccagcg cttctcccgc aaataccgtc tgatttcccg aaaatttttt
      121 aatctccttt gtctcttcta tataaacggc aaccgcatca ggacctgcaa aatcattaag
      181 cacatcaagc agctttgctt cctgcttctg ataatcgtcc cgtgtcagaa acttgatcca
      241 aagctttttc ggcatgtttg caaaaggtat gatactttca cagataagct ttgcgtcctt
      301 ttcctcttcc gcactgacac gtcccttgat aaaaaccttg gagtcctctg tcagccattt
      361 tacatttttc tcataatctc cgggccagat aatgacttcc accgttccaa caagatcctc
      421 cagcgtaata aacgccattg cacggccgtt ttttgtatgc ttgatcgtct tatgttcaat
      481 aattccgccg atcgtcgcct tcctgccgtc ctgcactttt gtgatccctg tttccgcatc
      541 cagcacaaag tccgaagtat atgcggtaat atgttttttc cacattgctt catccgcttc
      601 aagaggatgt ccgctgacat agatgccaag cacctccttc tcatagctga gcaaaagctc
      661 ttttccaaac tctcctacat ccggcagtcc gacttcaaat gtctccctgt cgtcttctcc
      721 tacaatgtca aacagcgaca gctgtcctgc caaattattc tttttatcct ggctgaccgt
      781 atccatgatc tgagtataca cattcataaa ctgctttctc gttccgccaa gactgtcaaa
      841 ggctcctgat ttgataaagc tctcgaccgt ccgcttattg acctctcttc cggagagacg
      901 caaggcaaaa tccttcagat ccttaaattt gccgtgctcc tttctctctt ccacgatcgc
      961 ctcgatcacg ggccagccaa tgctttttat ggaagcaagg ccgtaccgga tagagttgcc
     1021 ggagacagaa aagcccgcca ttccttcatt gatatccgga ggcagtatcg cgatccccat
     1081 agtcttgcac gtataaatat actccgccac tttaccggga ttatcaatga ccgatgtcat
     1141 cagcgcagcc atg
//
LOCUS       DAOSYE010000851         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_118179_length_1151_cov_2.049270, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000851 DAOSYE010000000
VERSION     DAOSYE010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118179_length_1151_cov_2.049270"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLU43_12075"
     CDS             <1..409
                     /locus_tag="PLU43_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29193.1"
                     /translation="PEGITTATVCNKSGKLAISGLCTADGSAITEYFATGTVPTEVCD
                     RHVLGQVCSLTGLRASDTCPYAVPGVILVDPNNDAYASTPYCVHSYLNGVPLFSIPDG
                     MTIEDFMLSTTTDAATTTDTTATDALNSLITTQ"
     gene            complement(481..>1151)
                     /locus_tag="PLU43_12080"
     CDS             complement(481..>1151)
                     /locus_tag="PLU43_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29194.1"
                     /translation="CAQIMMDKKIQEQTLYKGREEFQQTLDNLTAALKKQQYSYYKLN
                     MITQLLLTQMKEFTYLEMDEHQALYDLVNEIFTTMLKMQTTGAGNIGRFEETVNADLK
                     KIGREHPGFTTGEISSCLIGDILKVKASNIRFVIWMLARYGAAVMKECRLTVSSHFVT
                     ATKAMFEITLHGELSEKLPDVVEQMVQELLAAVSMSVKREEREGALVFHIEFLTPFAD
                     EAGE"
BASE COUNT          284 a          308 c          236 g          323 t
ORIGIN      
        1 accggaaggc attacgaccg ccaccgtctg caacaaatca ggaaagcttg ccatatccgg
       61 tctgtgtacg gcagacggca gtgccattac cgaatatttt gcgaccggta ccgtaccgac
      121 cgaagtctgt gacagacatg tgcttggcca ggtttgttcc ctgaccggac tgcgtgcttc
      181 cgatacatgc ccgtatgcgg ttcccggtgt cattcttgta gatccgaata atgatgccta
      241 tgcatcgacg ccgtactgtg tccacagcta cctaaatgga gttccgctgt tctcgatccc
      301 ggacggtatg acgatcgaag actttatgct cagtacgacg accgacgctg caacaacgac
      361 cgatactacg gcgaccgacg cactgaactc acttataaca acacaataat caataatagc
      421 aggtataaca aaagaacggc ttctccttcc tgatcaagga gaaaccgttc tttttatatt
      481 ttattcgccg gcttcatcgg caaatggcgt taaaaattcg atatggaata caagtgcgcc
      541 ttccctttcc tcccgcttaa cgctcatgga aactgcggca agcagctcct gcaccatctg
      601 ttccaccaca tccggcaatt tctcggaaag ctcaccatgc agcgtgatct caaacattgc
      661 tttcgtggcc gtcacaaaat gagaggaaac cgtcagcctg cactctttca tgacagcggc
      721 accatatctg gcaagcatcc agatcacaaa tctgatattg gatgctttta ctttcaggat
      781 atctccgatc agacagctgc tgatctctcc tgtcgtaaaa cccggatgtt ccctgccgat
      841 cttttttaaa tccgcgttga ctgtttcttc gaatcttccg atattaccgg cgccggtcgt
      901 ctgcattttc agcattgtcg taaatatttc atttaccaga tcatacagcg cctgatgctc
      961 atccatttcc agataggtaa actccttcat ctgtgtaagt aaaagctgcg tgatcatatt
     1021 gagtttataa tagctgtact gctgcttttt caatgcggca gtcagattat caagtgtctg
     1081 ctgaaactct tccctgcctt tatataaggt ctgttcctgt atttttttat ccatcatgat
     1141 ctgggcacat t
//
LOCUS       DAOSYE010000852         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_118183_length_1151_cov_2.039234, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000852 DAOSYE010000000
VERSION     DAOSYE010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118183_length_1151_cov_2.039234"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLU43_12085"
     CDS             complement(<1..740)
                     /locus_tag="PLU43_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO29195.1"
                     /translation="METITIKDIAKICGVGVSTVSRALNNHPDINPETRLRVMEVVEE
                     YNFIPNNSARNLKITDSKTIAILIKGISNPFFMKMIKVMEDEIQKKKYSLVLHHVDAN
                     EDEVDVAIELEKEKRLRGIIFLGGYFTHTQEKLERLQVPFVLSTITMTNGYRGNYSSV
                     AVDDVKESYKMVDYLCRLGHKRIATITATEADESIGKLRNEGYETALAANGIALDKKL
                     VYYMDTAIDGYTMENGYTVMKRIPVFHR"
     gene            complement(830..>1151)
                     /locus_tag="PLU43_12090"
     CDS             complement(830..>1151)
                     /locus_tag="PLU43_12090"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOO29196.1"
                     /translation="GTHDNDTLQSWYKNLSKQDKKLSQDYIGNADHPVEKINWDFIRL
                     AMGSVAKLCIIPMQDYLGLGSEARINMPATLGDNWKWRMKKGEFKKKLSKKVFEITKL
                     YGRL"
BASE COUNT          290 a          295 c          198 g          368 t
ORIGIN      
        1 atacgatgga aaacgggtat acgcttcatg accgtatacc cgttttccat cgtatagccg
       61 tcgatcgcag tgtccatata ataaacaagt tttttgtcaa gtgcgatgcc gttggccgca
      121 agcgccgttt catagccctc attgcgcagc tttccgatac tttcgtccgc ttccgttgcc
      181 gtgatcgtcg cgatccgctt atgtccgagt ctgcacagat aatctaccat cttgtagctt
      241 tcctttacat catctacagc gaccgacgaa taattgcctc tgtaaccgtt cgtcattgtg
      301 atcgtactga gcacgaacgg cacctgcaga cgctccagct tttcctgcgt atgggtaaaa
      361 tagccgccca gaaaaataat tcccctaaga cgcttctctt tttcaagctc tattgccaca
      421 tcgacctcat cctcatttgc atcgacatgg tgcagaacaa gggaatattt ctttttttgg
      481 atctcatctt ccatgacctt gatcattttc ataaaaaacg ggttggaaat cccttttata
      541 agaattgcga tcgttttgga atctgtaatt ttcaagtttc tggcgctgtt attcggaata
      601 aaattatatt cctccacgac ctccatgacc cgcagtcttg tctccggatt aatatccgga
      661 tggttgttca gtgcacggga cactgtactg acacctactc cacatatttt tgcaatatcc
      721 ttgattgtaa ttgtctccaa tttcagcacc tactttattc taacatagca aatgcaacct
      781 gaataagcat cacgcactat acttatccaa agttgcataa atccgctagt tacagcctac
      841 catataattt cgtgatttca aagacttttt tagataactt tttcttaaat tcaccctttt
      901 tcatacgcca tttccaatta tcccccagcg ttgcaggcat attgatccgc gcttcactgc
      961 caagaccaag ataatcctgc atcggaataa tgcagagctt tgccacgctg cccatcgcaa
     1021 ggcggataaa atcccagttt attttttcga ctggatgatc cgcattgcca atataatcct
     1081 gcgatagttt tttatcctgt tttgacaaat ttttatacca gctctgcaat gtatcattat
     1141 catgtgtccc c
//
LOCUS       DAOSYE010000853         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_118224_length_1151_cov_1.697080, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000853 DAOSYE010000000
VERSION     DAOSYE010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118224_length_1151_cov_1.697080"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLU43_12095"
     CDS             complement(<1..238)
                     /locus_tag="PLU43_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29197.1"
                     /translation="MKSNFNDFRKFVENQQRKQNSRGIDDFDGLSPNTMHNILYSTFT
                     KNGLIRFNADISAESINQVPVFKLVKHFMRILERE"
     gene            complement(341..>1151)
                     /locus_tag="PLU43_12100"
     CDS             complement(341..>1151)
                     /locus_tag="PLU43_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOO29198.1"
                     /translation="EAYPDYPYLNVPLSRKKGDIKKIPLDKITWFQHISLIGKVKNIK
                     EKAFYITESSNNGWNTEVMLTQIASGLYRRSGMAINNFSEALPEYQSDLAQSIFKDPY
                     KFGFLNLSRKVSEYDIEQKLTEKLSDFLIELGKGFAFVGRQYGIEVDGTDYKIDLLFY
                     HTILHCYIVIELKSGEFRPEYISKLNFYISAVDDILKTGNDKPTIGLLLCASKSNIKV
                     EYAMRGMKIPLGVAAYEIEELIKNSLPEKKQLKQLMVREIEAVYGSDDDRS"
BASE COUNT          285 a          259 c          220 g          387 t
ORIGIN      
        1 tttcccgctc aagtatccgc ataaaatgtt tgactagctt gaagacaggc acctgattaa
       61 ttgattctgc cgaaatgtcc gcgttgaacc ggatcagacc gttttttgtg aaggttgaat
      121 acaggatgtt gtgcatggta tttggagaaa gtccgtcaaa gtcgtcaatt cctctgctgt
      181 tctgctttct ctgctgattc tctacaaact tcctaaagtc attaaaattt gatttcatta
      241 ttgttctcct ttccgctgat ttaatgacat gtcttgtcct gcattttggg gcgttttaca
      301 gccttggtta cgcacaattc tgcaagtgag acttgcacaa tcaggaacgg tcatcatcgc
      361 ttccgtaaac tgcttcgatc tcccttacca tcagctgttt cagctgtttt ttttcaggca
      421 gactgttctt gatcagttct tctatttcat acgcggcgac tccgagaggt attttcatcc
      481 ctctcattgc gtattccacc ttgatgttgc tttttgacgc gcaaagcaga aggcctattg
      541 tcggtttatc atttccggtt tttaatatat cgtcaacggc gcttatataa aaattcagct
      601 tagaaatata ctccggtctg aattctcctg atttgagttc gatcacgatg tagcagtgaa
      661 gaatagtgtg ataaaataga agatctatct tatagtccgt accatcaacc tctatcccgt
      721 actggcggcc tacaaaagca aaacccttcc ccagctctat cagaaagtca gacagctttt
      781 ccgtcagctt ctgctcgata tcatattcgg acacttttct gctgaggttg agaaatccga
      841 atttatacgg gtctttaaaa atactttgcg cgagatccga ctgatattcc ggaagagctt
      901 cagagaaatt atttattgcc attccgcttc ttctgtaaag tcccgaagcg atctgtgtga
      961 gcatcacttc cgtattccat ccgttattgg aagattccgt tatatagaaa gccttttcct
     1021 taatattttt gacttttccg atcagagaaa tatgctgaaa ccatgtgatc ttatccagcg
     1081 gtattttttt aatatcacct ttctttctgg acagcgggac attcagataa ggataatcgg
     1141 gatacgcctc c
//
LOCUS       DAOSYE010000854         1149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_118542_length_1149_cov_1.772395, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000854 DAOSYE010000000
VERSION     DAOSYE010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118542_length_1149_cov_1.772395"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..>1149
                     /locus_tag="PLU43_12105"
     CDS             335..>1149
                     /locus_tag="PLU43_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOO29199.1"
                     /translation="MTKEKKIPYKIYLEEHEMPTKWYNMRADMKNKPAPLLNPGTLQP
                     MTVEELSGVFCTELAKQELNDTDAYIEIPEEIQNFYKMYRPSPLVRAYCLEKKLQTPA
                     KIYYKFEGNNTSGSHKLNSAIAQAYYAKKEGLKGVTTETGAGQWGTALSMACSYFDLD
                     CKVYMVKVSYEQKPFRREVMRTYGASVTPSPSMTTQVGKKILAEHPGTTGSLGCAISE
                     AVEVAVGTEGYRYVLGSVLSQVLLHQSVIGLETKAAMDKYGVKPDLIIGCAGGG"
BASE COUNT          339 a          229 c          325 g          256 t
ORIGIN      
        1 actaaggcga atgggcttct gagggcgaac cgaacttgcg gcaggacttt gtgaaagcag
       61 atgctgccgg gaaacaggta ggggagacgg agagacactc cgttaacaac cgcagcatat
      121 tgttatatga tagaggccgt tgtaatgaca gcggactgat atcggtagta tgcatgagag
      181 gacgcacagc gtcaagccgg gtggcaccgc aggagagact ataactcttg tcccagtaga
      241 aactattggg ataagagttt tttcatattt tcttatccca tgaacctgtc cggagcttcg
      301 gacatttgta ttcaaaagaa aaggagagaa aacaatgaca aaagaaaaga aaattcctta
      361 caaaatctat ctggaagagc acgagatgcc gacaaagtgg tataatatgc gtgcagacat
      421 gaaaaataag cctgcaccgc tgctgaatcc gggtacatta cagccgatga ccgtggaaga
      481 gctgagcggt gtgttctgta cggagcttgc aaagcaggaa ctgaacgata cggatgctta
      541 tattgagatt ccggaagaga tccagaattt ttataaaatg tatcggccgt caccgcttgt
      601 aagggcatat tgtctggaaa agaaactgca gacaccggca aagatctatt ataaattcga
      661 aggaaacaat acgagcggca gccataagct caattctgcg atcgcgcagg cgtattatgc
      721 aaaaaaagaa ggtcttaagg gcgttacgac ggaaacgggt gccggacaat ggggaaccgc
      781 gctttcgatg gcctgctctt attttgactt ggactgtaaa gtatatatgg taaaagtatc
      841 ttatgaacag aagccgttca gacgtgaagt gatgcgtact tacggggcca gcgtgacacc
      901 gtcgccgtcc atgacgacgc aggtcggcaa aaagatcctt gcagagcatc cgggaacgac
      961 aggcagtctt ggctgcgcga tttcggaagc agtagaggtg gctgtcggta cggaagggta
     1021 ccgttatgta cttggaagcg ttttgtcaca ggtacttctg catcagtcgg tcattggact
     1081 tgaaacaaag gctgcgatgg ataagtatgg tgtaaaaccg gatctgatca tcggctgtgc
     1141 cggcggcgg
//
LOCUS       DAOSYE010000855         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_119422_length_1144_cov_1.716253, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000855 DAOSYE010000000
VERSION     DAOSYE010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119422_length_1144_cov_1.716253"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1144
                     /locus_tag="PLU43_12110"
     CDS             <1..>1144
                     /locus_tag="PLU43_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29200.1"
                     /translation="IALSLLGLPCREFRDKYRTKDFCIWMFSMEDKKEIVSDVFEKFY
                     EQFGFYPESTGSYYMDAELINYIKEKYPMIKCAVATCWEEGPKAYHTCNNSWYTLFDG
                     GPWNPWIPSKQNTHAPAADQSEDSGIVAIPHLSRDLIACYDGNGSNFGTHPQNVLRGM
                     IYDSEKWEYPYLYNLIDQYRHLAKFNNGYSYNMMFVGPGWMNKMGRWEAPYELLKKSY
                     DDGMAYYAKLKAGGALVDMTMSEFAGYYRHKKTYTEPECALWRDILYGSKKQVFWYTD
                     PFMRVCVNMDQGGAIVDLRPYAAKLHWPVGIGTKHVQDASYPFLIQEKYRAGFFTHYA
                     GEGTIRSAKLSYKGEEADLCLCRTKAVFSEETLTGDKKTRILTLKPV"
BASE COUNT          328 a          222 c          312 g          282 t
ORIGIN      
        1 aatcgcactg tccctactgg gacttccgtg cagggaattt cgggataaat accggacaaa
       61 ggacttttgc atctggatgt tttccatgga agataagaaa gagatcgtca gcgacgtgtt
      121 tgaaaaattt tatgagcagt ttggttttta tccggagtca acaggttcct attatatgga
      181 tgcggaactg atcaattata taaaggaaaa gtatccgatg atcaagtgtg cggtcgcaac
      241 atgctgggag gaaggaccga aagcatatca tacgtgcaac aattcctggt atacgttatt
      301 tgacggagga ccatggaatc cgtggatccc ctcaaagcag aatacacatg cgccggcagc
      361 cgatcagtct gaagacagcg gtatcgtggc aattccgcat ctgtcgaggg atctgatcgc
      421 atgctatgac ggcaatggct ccaatttcgg gacacatccg cagaatgtac tgcgcggaat
      481 gatctatgac agtgagaaat gggaatatcc ttatttgtat aatctgatag accagtaccg
      541 gcatcttgcg aaatttaata acggctattc ttacaatatg atgttcgtag gtcctggctg
      601 gatgaataag atggggcgct gggaggcacc gtatgagctg ctgaaaaaat cctatgacga
      661 cggaatggca tattatgcaa agctgaaggc agggggagcg cttgtcgata tgacgatgtc
      721 ggaatttgcc ggctattatc gtcataaaaa gacatatacg gaaccggaat gtgctttatg
      781 gagggatatt ctctatggtt ccaaaaaaca agtgttctgg tatacggatc cgtttatgcg
      841 tgtatgcgtg aatatggatc agggaggagc gatcgtagat ctgcggccgt atgcggcaaa
      901 gctgcattgg ccggtgggga tcgggacaaa gcatgtacag gatgcatcct acccgtttct
      961 gatccaggaa aaataccgtg cgggattttt cacccattat gcaggagagg gaacgatacg
     1021 cagtgcaaag ctctcctata aaggggaaga agcggatctg tgtctgtgcc gcacaaaggc
     1081 ggtattcagt gaagaaacat tgacaggaga taaaaaaacc cgtattctga cactgaagcc
     1141 tgtt
//
LOCUS       DAOSYE010000856         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_119722_length_1142_cov_2.372585, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000856 DAOSYE010000000
VERSION     DAOSYE010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119722_length_1142_cov_2.372585"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLU43_12115"
     CDS             <1..634
                     /locus_tag="PLU43_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29201.1"
                     /translation="LENEVHSSAFQDAGYRIVMFHHAPHGLGGNIIPPFTDPVCREVY
                     DADGQLSQILYDYPKEDDDLINDLVPLLQQAGTDLVLNGHSHIWNRFRDQCGMNYLET
                     SNVGNSYGAYDGIADEDSDGTRTDRIPSDMTYFNAANYVLSGDPGGLEPCMPTIRSLD
                     GKPYIASNTVTVFSLFDTATGIVESYWYDVTKPEEKPVKFDSFQAKRILP"
     gene            complement(647..>1142)
                     /locus_tag="PLU43_12120"
     CDS             complement(647..>1142)
                     /locus_tag="PLU43_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HOO29202.1"
                     /translation="KTKSYREKCNVIAHSYQKEVISRMKRKATIMDEQKNIFQSLVQD
                     LTSSMNIDIERILSVYLKKTPSLECIYILDENGIQTTDTVFNRNIKIKNEKMFSPTQS
                     GDYHISKQYYYYVITNTDKIYISSKYVSQATGNLCRTISSLFVNKEGKKIVCVDFSAE
                     KNRE"
BASE COUNT          317 a          248 c          233 g          344 t
ORIGIN      
        1 gctggaaaac gaagtacaca gttctgcctt tcaagatgcc ggctatcgca tcgtcatgtt
       61 ccaccatgca ccgcatgggc ttggcggcaa tattatacct ccctttacgg atcctgtctg
      121 ccgggaagtg tatgacgcag acggacagct ttcacagatc ctgtatgact atccaaaaga
      181 agatgatgat ctgatcaatg atctggtccc ccttttgcag caggccggaa cagatctcgt
      241 cttaaacgga cattcccata tctggaaccg gtttcgggat caatgcggca tgaattatct
      301 ggaaacttcc aatgttggaa attcctatgg ggcttatgac ggaatcgccg atgaagacag
      361 cgacggaacc cgaaccgatc ggattccgtc tgatatgact tattttaatg cggcaaatta
      421 tgttctgtcc ggcgatcccg gcgggttgga gccatgcatg ccaacgatcc gttcactgga
      481 cggcaagccg tatatcgcca gcaataccgt tacggtcttc tcgctttttg acacggcaac
      541 cggcattgtg gaaagctatt ggtatgatgt gaccaagccg gaagaaaaac cggtaaaatt
      601 tgattccttt caggcaaaaa ggatccttcc atagatccct gatcaattat tcgcgatttt
      661 tttctgctga aaaatcaaca catacgatct ttttcccttc tttgttaaca aaaagagaag
      721 aaatcgtacg gcataaattc cctgtagcct gagaaacata tttagaactg atatagatct
      781 tgtccgtatt ggtaatcaca taataataat actgtttgga aatatgataa tctccgcttt
      841 gcgtaggcga aaacatcttt tcatttttaa ttttgatatt gcggttgaaa acagtatctg
      901 ttgtctgtat tccgttttca tccaaaatat aaatgcattc cagcgaagga gtttttttta
      961 aataaaccga taatattcgc tcaatatcaa tattcatgga agaagtcaga tcctgaacca
     1021 gcgattggaa tatatttttc tgctcatcca tgatcgtagc ctttcgcttc atccgtgata
     1081 ttacttcttt ctgataggaa tgtgcaataa cattacactt ttcacgatat gattttgttt
     1141 tg
//
LOCUS       DAOSYE010000857         1139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_120264_length_1139_cov_2.821956, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000857 DAOSYE010000000
VERSION     DAOSYE010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120264_length_1139_cov_2.821956"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLU43_12125"
     CDS             <1..757
                     /locus_tag="PLU43_12125"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOO29203.1"
                     /translation="GLLPDALLLIDNPDREVATAFMKLNQYVDLLIPRGGAGLIRSVL
                     KNATIPVIETGTGNCHIFVDETADIAMAVDIIDNAKTQRMGVCNACESLVVHEKAADK
                     AIPAIYERLTGKQVEIRAGERAFMICGSTCGKMVPATEEDWGTEYLGAIISLRVVSDV
                     EEAIAHINRYTTHHSEAIITNNMENAEKFLNEVDAAAVYVNASTRFTDGFEFGFGAEI
                     GISTQKMHARGPMGLEALTSIKYKIYGTGQVRP"
     gene            820..>1139
                     /locus_tag="PLU43_12130"
     CDS             820..>1139
                     /locus_tag="PLU43_12130"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOO29204.1"
                     /translation="MRKVVLAGACRTPIGTMGGELSKVSAADLGTIVIKEALRRSGVK
                     PEQVDEVYMGCVLQAGQGQNVARQASVNAGIPVEVPATTINVLCGSGLHCVNLAAKLI
                     AFGDA"
BASE COUNT          302 a          226 c          349 g          262 t
ORIGIN      
        1 agggcttctg ccggatgcat tgcttttgat cgacaatccg gacagggaag ttgctaccgc
       61 attcatgaaa ctgaatcaat atgtggatct gctgattcca aggggcggtg cgggactgat
      121 ccgttccgta ctgaaaaatg caacgattcc cgtgatcgaa acggggacgg gcaactgtca
      181 catttttgtg gatgaaacgg cagacattgc aatggcggtc gatattattg acaatgcaaa
      241 aacacagcgt atgggcgtct gcaatgcctg tgagtcgctg gtcgttcatg aaaaggcggc
      301 cgacaaagcg atccccgcaa tttacgagcg tctgacaggg aaacaggtgg agatccgcgc
      361 aggagaaaga gcgtttatga tctgcggcag cacatgcggg aagatggttc ctgcaacgga
      421 agaggactgg ggaacggaat acctgggcgc aattatttct cttcgggtcg tgtctgatgt
      481 tgaagaagcg atcgcgcata tcaaccggta tacaacacat cattctgagg cgatcatcac
      541 gaataatatg gaaaatgcgg agaaattttt gaatgaagtg gatgcggcag ctgtgtatgt
      601 caatgcatcg acgcgtttta ccgatggatt tgaatttgga tttggggcgg agatcggcat
      661 cagtacacag aaaatgcatg caagaggacc gatggggctg gaagcgctta cttcgatcaa
      721 atacaagatt tatgggacgg gtcaggtacg tccgtagcag ctgggataaa ggcagttaat
      781 ctgcctttat atagactcaa aaagaaaaga ggttttttta tgagaaaagt agttttggca
      841 ggagcatgcc gtacaccgat cggtacgatg ggaggcgagc tcagtaaagt gtcggcggcc
      901 gatctgggga cgatcgtgat caaagaggcg ttaaggcgca gcggtgtaaa accggaacag
      961 gtcgatgaag tttacatggg ctgtgttctg caggcagggc agggacagaa tgtggcgcgt
     1021 caggcgtccg tcaatgcagg aattcctgtt gaggttccgg cgacaacgat caatgtgctg
     1081 tgcggttccg gacttcactg cgttaatctg gcggcaaagc tgatcgcttt tggagatgc
//
LOCUS       DAOSYE010000858         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_120953_length_1135_cov_3.834259, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000858 DAOSYE010000000
VERSION     DAOSYE010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120953_length_1135_cov_3.834259"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..664
                     /locus_tag="PLU43_12135"
     CDS             92..664
                     /locus_tag="PLU43_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO29205.1"
                     /translation="MKNFTAKQIAQTALLLALCIISQYFKSLSVYITGPIVNAILVIA
                     TLAVGPVSGIIISIIAPITAFFFTGSPIMAALPVMFPIIMISNTILVLFTYYFEKKKS
                     FRFHLETGLILGSIVKAAFLGITVVLILLPYFGNAIAAKLPKPEALPKVLAAAKITFS
                     VTQLITALIGSLIAWLIWHRLKKAIVNTDI"
     gene            complement(722..>1135)
                     /locus_tag="PLU43_12140"
     CDS             complement(722..>1135)
                     /locus_tag="PLU43_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOO29206.1"
                     /translation="GITVKTDTKIKDVDIKQAGLLVLPGGMPGTKNLAACGKLTEMLR
                     QFHTTNKRIAAICAAPSVLGQLGLLKGKKAVCYPGYEEQLTGAEILYEEVVTDGNITT
                     SRGLGTAIPFALELIRQIDCETEAGAMKTKIVYKQ"
BASE COUNT          282 a          306 c          226 g          321 t
ORIGIN      
        1 gactcggaag acttgacaat gtcacagcaa aatccatgca ggcaacaata taagcaggca
       61 gctgccctgc aaatcacagg aggatatcat catgaaaaat tttacggcaa aacaaattgc
      121 ccagacagcg cttcttcttg cgctctgtat catcagtcag tattttaaaa gcttatccgt
      181 atatattaca ggaccgatcg tcaatgcgat ccttgtcatc gcaacgcttg cggtcggccc
      241 ggtttccggt attattattt ccattatcgc gccgatcact gcctttttct ttaccggctc
      301 cccgatcatg gcagcacttc cggtcatgtt tccgatcatt atgatcagca acacgatcct
      361 tgtactgttt acctattatt tcgaaaaaaa gaaatccttc cgcttccatc tggagacggg
      421 actcatactt ggctctatcg taaaagccgc attcttaggt atcacggtcg tattgatcct
      481 gcttccgtac ttcggaaatg ccattgctgc aaaacttccg aaaccggaag cgcttccaaa
      541 agtgcttgca gctgcaaaaa tcacattttc agtcacacag ctgattaccg cactgatcgg
      601 cagcctgatt gcctggctga tctggcacag gcttaaaaaa gccatcgtca atacggatat
      661 ttaagatccc ggacgagagg aaaaggaatg ccctggcggc attccttttc cattgtcctg
      721 cttactgttt atatacgatc tttgttttca tggcaccggc ttctgtttca caatcgatct
      781 gcctgatcag ttcaagtgca aagggaattg ccgtaccaag tcctctggat gtcgtaatat
      841 ttccatccgt tacaacttcc tcatacaaaa tttcggcacc ggtcagctgt tcctcataac
      901 ccggatagca gacagctttt ttccctttta aaagaccaag ctgcccaagt acggaaggtg
      961 ccgcacagat cgcagcgatc cttttgttcg tagtatgaaa ctgccgcagc atctctgtca
     1021 gcttcccgca ggccgccagg tttttcgttc ccggcatccc gcccggcagt acaagaagcc
     1081 ctgcctgttt tatatcaacg tcctttattt ttgtatcggt ctttaccgta atacc
//
LOCUS       DAOSYE010000859         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_121141_length_1134_cov_3.238184, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000859 DAOSYE010000000
VERSION     DAOSYE010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121141_length_1134_cov_3.238184"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLU43_12145"
     CDS             complement(<1..138)
                     /locus_tag="PLU43_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOO29207.1"
                     /translation="MFDRLEDLIIRYEEIMSELNEPTVMENQQRFRALMKEQSDLAPI
                     VD"
     gene            complement(157..1005)
                     /gene="prmC"
                     /locus_tag="PLU43_12150"
     CDS             complement(157..1005)
                     /gene="prmC"
                     /locus_tag="PLU43_12150"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOO29208.1"
                     /translation="MCSYRDLYKSGETRLKAAGIAESSLDARLLLEFVMGTDRNYLLA
                     HGEEPVDSFHIRAYEELIELRAAHTPLQYLTGVQEFMGLIFAVDERVLIPRQDTECLV
                     EEAMIETADGMRVLDLCTGSGCIIISLMKYKNEIAATAVDISPDALAVAKKNADIHQV
                     SVNFVESDLFAGLPVSETALFDVIVSNPPYIKTAVIETLSGEVKEHEPRLALDAADDG
                     LYFYRKIISAAGEYLVRGGKLLFEIGYDQGKQVCKLMEEHGFADIRTVKDLAGLDRVV
                     CGTYRK"
     gene            complement(998..>1134)
                     /locus_tag="PLU43_12155"
     CDS             complement(998..>1134)
                     /locus_tag="PLU43_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOO29209.1"
                     /translation="KLTTREPDDDMIEVAIAAVEAVFDWKDFLRTEFGYDVGGREADV
                     "
BASE COUNT          282 a          352 c          200 g          300 t
ORIGIN      
        1 atcaacgatc ggtgccagat cgctctgttc cttcatcaat gcacgaaaac gctgctgatt
       61 ctccatcact gtcggctcgt tcagctcgct cataatttcc tcatatctga taatcaaatc
      121 ctcaagcctg tcaaacattt ctttcctccg aactatttac ttcctatagg taccgcagac
      181 taccctgtca agacccgcaa ggtcctttac cgtcctgata tctgcaaatc cgtgctcctc
      241 catgagtttg caaacctgct tcccctggtc atatccgatc tcaaataaca gcttcccgcc
      301 gcgcaccaga tattccccgg cagctgaaat gatcttcctg taaaaataca gaccgtcgtc
      361 tgccgcatca agtgccagcc ttggctcatg ctcctttact tccccgctca gtgtttcgat
      421 cacagccgtt ttaatatacg gcggattgga tacgatcaca tcaaacagag ccgtctcaga
      481 taccggaagt ccggcaaaca gatcgctctc tacaaaattc acagagacct gatgtatatc
      541 ggcatttttt ttcgcgaccg caagcgcatc cggtgaaata tccacagctg tcgcagcgat
      601 ctcattttta tatttcatta agctgatgat aatacagccg gaaccggtac acagatcaag
      661 cacccgcatt ccgtcagctg tctcgatcat agcctcctcg acaagacatt ctgtgtcctg
      721 ccgcggtatc agcacccgtt catccactgc aaaaatgagt cccataaatt cctggacccc
      781 tgtcagatac tgcaatggag tatgtgcggc ccgcagttcg atcagttcct catatgcgcg
      841 gatatgaaaa gaatccaccg gctcttctcc atgagccagc agatagtttc tgtccgttcc
      901 cataacaaac tccagcagca gcctcgcatc caaagatgac tctgcgattc ctgccgcctt
      961 taaacgcgtt tcaccgcttt tgtataaatc acgatagcta cacatctgct tccctgccac
     1021 cgacatcata tccaaattca gtcctcaaaa aatccttcca gtcaaatacc gcttccacag
     1081 ctgcgatcgc aacctctatc atatcgtcat caggttccct tgtcgtcagc ttct
//
LOCUS       DAOSYE010000860         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_121535_length_1132_cov_3.362117, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000860 DAOSYE010000000
VERSION     DAOSYE010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121535_length_1132_cov_3.362117"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..413)
                     /locus_tag="PLU43_12160"
     CDS             complement(90..413)
                     /locus_tag="PLU43_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO29210.1"
                     /translation="MLMIRAIVRPEKAGYIMAELAEAGFPAVTKMDVFGRGRQKGIMV
                     DDYCYDEIPKEMLLIVCNEEDKDTIVRIILRTAKTGEGNFGDGRIFVTAVEEAYTVSN
                     GKAGL"
     gene            complement(438..>1132)
                     /gene="nifH"
                     /locus_tag="PLU43_12165"
     CDS             complement(438..>1132)
                     /gene="nifH"
                     /locus_tag="PLU43_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447878.1"
                     /note="nitrogenase iron protein; nitrogenase component 2;
                     with component 1, an molybdenum-iron protein, catalyzes
                     the fixation of nitrogen to ammonia; nitrogen reductase
                     provides electrons to the nitrogenase complex; in R. etli
                     there are three essentially identical copies of nifH which
                     are actively expressed during symbiosis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase reductase"
                     /protein_id="HOO29211.1"
                     /translation="STRLILGGLAQKTVLDTLRDEGDDIELDAVLKTGFKGIRGVESG
                     GPEPGVGCAGRGIITSINLLEQLGAYTDDLDYVFYDVLGDVVCGGFAMPIREGKAQEI
                     YIVCSGEMMALYAANNISKGIAKYGRTGTVRLGGLICNSRKVDRESELVEAVAKKLGT
                     QMIHFVPRDNAVQKAEIHKKTVIDFSPEEPQAQEYRDLARKIEENDMFVVPTPMAIDE
                     LEEILMEYGIMD"
BASE COUNT          264 a          327 c          195 g          346 t
ORIGIN      
        1 ccggcctctg caagcgcgac tttcgttgca tttactttat tggcacgaat aaaagccatt
       61 atctctttca tagggttacc tgcctttcct taaagtcctg cttttccgtt acttaccgta
      121 taagcttcct ccaccgcagt tacaaatata cggccatcgc cgaagtttcc ttcgccggtc
      181 tttgctgtac gtaaaataat tctgacaatt gtatccttat cttcttcatt gcagacgatc
      241 agcagcatct cctttggaat ctcatcataa caataatcat ctaccataat acctttttgt
      301 ctgcctcgtc cgaatacgtc catttttgtc actgccggaa atcctgcttc tgccagctct
      361 gccataatat aacccgcttt ttcgggtctg acgatagctc tgatcatcaa cataggtgta
      421 tcctccttca tctgtaatta atccatgatt ccatattcca tcagaatctc ttccaactca
      481 tcgatcgcca tcggcgtcgg tactacaaac atgtcatttt cttcgatctt tcttgcaagg
      541 tcacggtatt cctgtgcctg cggctcttcc ggactgaaat caattacggt ctttttatgg
      601 atctccgctt tctgcaccgc attgtcacgc ggaacaaaat gtatcatctg cgttccgagc
      661 tttttcgcta ccgcttccac aagttcagat tccctgtcta ccttacggga attacagatc
      721 agtcctccca gacgaacggt tccggtcctt ccgtacttgg caataccttt tgaaatatta
      781 ttcgccgcat acagcgccat catctcgccg gaacatacga tataaatttc ctgtgccttg
      841 ccttcgcgga tcggcattgc aaaaccaccg cacacaacgt ctccaagtac atcataaaat
      901 acataatcca gatcatccgt ataagcacca agctgttcga gcagattgat cgatgtgatg
      961 attccgcgtc cggcacagcc gacacctggt tccggaccgc ctgattccac cccgcgtatt
     1021 cctttaaagc ctgttttcaa tactgcatcc agttcaatgt cgtcgccttc gtctctcaat
     1081 gtatccagca ctgtcttctg ggcaagacca ccaagaataa gtcttgtgga at
//
LOCUS       DAOSYE010000861         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_122339_length_1128_cov_3.187325, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000861 DAOSYE010000000
VERSION     DAOSYE010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122339_length_1128_cov_3.187325"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="PLU43_12170"
     CDS             complement(<1..>1128)
                     /locus_tag="PLU43_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO29212.1"
                     /translation="ECFEDNNVIIPMVVIEELDGLKKAEGDKGASAREAIRSIEKYRD
                     QGDLFDGIQLKKGGILKIDANCVKVELPEALPADKPDNRILKVCKFHKEQGEQIILVT
                     KDLVLRIKADVLGVAAEDFTTEQVPKTIEQYTGREECYVAEEIFKEFRKKGVYRDQVY
                     LTDESENTYTPELFENQFVILKADQSLKRTQLGRVEGDRIVPLDYKKNKPYGITPRNV
                     GQYFLQEALMQPAEKAPLVIVKGMAGTAKTFYSLVVGLERLWNNPTGEYRKILVTRPN
                     AQFDSDIGFLPGSEQEKISPLMRPIIDNLEQIIDSNEEDRYKNEEELHGKIEEIFTRG
                     IIQTEAMNFIRGRSFVKTYLIVDEAQNMTPSQIKGIITRAGK"
BASE COUNT          243 a          309 c          202 g          374 t
ORIGIN      
        1 cttccctgct cttgtaataa ttcccttgat ctggcttggc gtcatgttct gtgcttcatc
       61 aacgatcaga tacgttttta caaaagaacg gcccctgata aaattcattg cctctgtctg
      121 tatgattcct cttgtaaaaa tttcttctat ttttccatga agttcttctt cattcttata
      181 ccggtcttcc tcgttggaat caatgatctg ctccaaatta tcaatgatcg ggcgcatgag
      241 cggagagatc ttctcctgtt cgctgcccgg cagaaacccg atgtcactgt caaactgcgc
      301 attcggtctg gttacaagaa tctttctgta ctctccggtc ggattgttcc acaatcgttc
      361 cagtccgact acaagggaat aaaatgtttt tgcagtgccg gccattcctt tgacaatgac
      421 aagcggggct ttctctgcag gctgcatcag agcttcctgt aaaaaatatt gcccgacatt
      481 gcgcggcgtg attccatatg gtttattttt cttataatca agaggtacga tccggtctcc
      541 ttccaccctt ccaagctgtg tccgcttcag cgactgatcc gccttcaaaa taacaaattg
      601 attttcaaac agctccggcg tataagtgtt ttcgctttca tccgtcagat agacctgatc
      661 tctgtaaacc ccttttttcc gaaactcttt aaaaatttcc tctgcaacat agcactcctc
      721 tcttcccgtg tactgttcga tcgtttttgg cacctgctcc gtcgtaaaat cttccgcagc
      781 cactccaagt acatccgctt tgattcgaag caccagatcc tttgtcacaa ggatgatctg
      841 ctccccctgc tctttatgaa atttgcatac cttcagtatc ctgttgtcgg gtttatctgc
      901 cggcaatgct tccggaagtt ccaccttgac gcagtttgcg tcgattttta aaattcctcc
      961 cttttttaat tgaatgccgt caaacaagtc tccctgatcg cgatactttt ctatgctgcg
     1021 gatcgcttct ctggcgcttg cccccttgtc cccctctgcc tttttcaatc cgtccagttc
     1081 ttcaatgact accatcggaa tgatcacatt attgtcttca aagcattc
//
LOCUS       DAOSYE010000862         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_122944_length_1125_cov_2.445794, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000862 DAOSYE010000000
VERSION     DAOSYE010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122944_length_1125_cov_2.445794"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLU43_12175"
     CDS             <1..846
                     /locus_tag="PLU43_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29213.1"
                     /translation="GTELIELFLNKNKRLQIMLLFDEADTERVVQMHNNYHLCKIFCK
                     NIFALDELPRHIEDALFSYNKDEELKKIELSYKEKEEKYKKTMFEMSTLLNDRMESYQ
                     EVIRHFMILSRYLLENTYTVDSSNYIGDILNSQKQILQNFVQIYLLRTPKQDGYFETA
                     GVRSNHPEIKKYFKVFNEVRTVIDDEIFQNMAFLLGTIMTYFTYYYSEYRGKAIVTKN
                     EQFYLLNIVYEVKQSEKMKKAGLELFALNERIVSSYAFKAAYGSRDAVMQFKIYFAGP
                     EMKEA"
     gene            864..>1125
                     /locus_tag="PLU43_12180"
     CDS             864..>1125
                     /locus_tag="PLU43_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29214.1"
                     /translation="MVDKLEKAKERDRNKIAEIRSKWNLDKIEDVEKVLGLLQTGSIW
                     FETETGRAFDDELYDRKLKLEGGHTSSAAVRGKKKNAGAANKK"
BASE COUNT          408 a          147 c          261 g          309 t
ORIGIN      
        1 ggtacggaac tgatagaact ttttctgaat aaaaacaagc ggcttcagat catgctttta
       61 tttgatgaag cggatacgga gcgtgttgtg cagatgcata ataattacca tttgtgtaaa
      121 atattctgta aaaatatttt tgcgcttgat gaattgccaa gacatattga agatgccctg
      181 ttcagctata ataaagatga ggaattgaag aaaatagagc tttcctacaa ggaaaaggaa
      241 gagaaatata aaaaaacgat gtttgaaatg tcaacgttgt taaatgacag aatggaaagt
      301 tatcaggaag ttatccggca ttttatgatt ttaagcagat atcttttgga aaatacatat
      361 accgtagatt cctcaaatta tattggtgat attttaaata gtcaaaaaca gattctgcag
      421 aattttgtgc agatttatct gcttcgtacc ccaaaacagg atggctattt tgaaacggcc
      481 ggcgtaagat ccaatcatcc ggaaataaaa aaatatttta aagtgtttaa tgaagtaaga
      541 acggtaattg atgatgagat ttttcaaaat atggcgtttt tacttgggac gatcatgaca
      601 tattttacgt attattattc ggaatacaga ggaaaagcga ttgtcacgaa aaatgagcag
      661 ttttatctgc ttaatattgt gtatgaagtg aaacaatccg aaaaaatgaa aaaagcaggc
      721 ttggaactgt ttgcattgaa cgaaaggatt gtttcttctt atgcgtttaa agcagcatat
      781 ggaagcaggg atgcggtaat gcagttcaag atatattttg ccggaccgga aatgaaagaa
      841 gcctagaaag aggaacgatt gcagtggtcg acaaactgga aaaagctaag gaacgggaca
      901 ggaataaaat tgcagagatc cgttccaagt ggaatcttga taaaatagaa gatgtggaaa
      961 aggtgcttgg acttttacag acaggaagca tttggtttga gacagaaaca ggacgagcgt
     1021 ttgatgatga attatatgac agaaaattga aactggaagg tgggcatact tcttccgcag
     1081 cagtcagggg gaagaagaaa aacgcgggtg cagcaaataa aaagg
//
LOCUS       DAOSYE010000863         1124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_123159_length_1124_cov_2.144060, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000863 DAOSYE010000000
VERSION     DAOSYE010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123159_length_1124_cov_2.144060"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..>1124
                     /locus_tag="PLU43_12185"
     CDS             248..>1124
                     /locus_tag="PLU43_12185"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO29215.1"
                     /translation="MNVTGIIAEYNPFHNGHLYQLTKAREETDADYMIVVLSGDFVQR
                     GTPAIADKYIRTKTALLAGADLVLELPVYFSSSSADFFAAGAVSLLDKIGCVDHLSFG
                     SEHGSIKELSDIAGHLLSETPSYKTVLKKHLKQGNSYALASAKASMEQFSDSRMAETL
                     LKEPNNTLGIAYLKALSGRNSTITPHTVLRTGGGYHATEKGALSASAVRHTVQTDGSL
                     DSVSAQMPAYAYDLLADTYGTSFPIFENDFSSLLAYKLRTILSNARLSAEDNSSPAHA
                     LTCYADVSEELACRIN"
BASE COUNT          335 a          254 c          241 g          294 t
ORIGIN      
        1 attccattta tattgattat aagtcaagca tgaaacgcag gcaatagata aaatggtgtt
       61 ttttgtgcaa aatcaagtac acggaacact aagaccgtta aatttttatc aatattgacg
      121 gttttatgta tacattatac aggaattgca gggtttcatt gcaacgatga aaaaaacagg
      181 ctatactgaa gaaaaatcaa tactatgaaa gagcaggcat gcacaggcat gacactgagg
      241 gtatcctatg aatgttaccg gaatcattgc cgaatataat ccatttcata acggtcatct
      301 ttatcagctg acaaaagcac gtgaagaaac agatgcagac tatatgatcg tcgttctttc
      361 cggcgatttt gtacaaagag gaacgcctgc cattgcagac aaatatatcc gtaccaaaac
      421 agctctgctt gcaggagccg acctcgtatt agaacttccc gtttactttt cctcctccag
      481 tgctgatttt tttgcagcag gagctgtttc cctgttagac aagatcggct gtgtcgatca
      541 tctaagcttt ggcagtgaac atggcagcat aaaagagctt tcagatattg ccggtcatct
      601 gctgtcggaa acaccttcct ataaaacagt attaaaaaaa catctgaagc agggaaactc
      661 ctatgcgctt gccagcgcaa aggcttccat ggaacagttt tcagacagcc gcatggcgga
      721 aacgctgcta aaggaaccga acaacacact tggtatcgct tatctcaaag cattatcagg
      781 gaggaacagt acgatcacgc cgcatacggt tcttagaacc ggcggcggtt accatgcgac
      841 cgaaaaaggg gctctttccg cttctgcagt cagacatacc gtgcagacag acggcagtct
      901 tgattctgtc agcgcgcaga tgcccgcata tgcttatgat ctgcttgcag atacatatgg
      961 aacatcattt ccgatttttg aaaatgattt ttccagtctg ctcgcttata aactgcgtac
     1021 aattctttca aacgcacgcc tgagcgctga agataacagt tcacctgccc atgcgctgac
     1081 ctgttatgcg gatgtatcgg aagaactggc ctgccgcata aacg
//
LOCUS       DAOSYE010000864         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_123678_length_1121_cov_3.228893, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000864 DAOSYE010000000
VERSION     DAOSYE010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123678_length_1121_cov_3.228893"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLU43_12190"
     CDS             complement(<1..672)
                     /locus_tag="PLU43_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO29216.1"
                     /translation="MGGFLDYIPGNSLLHKLNPLTKLLAAFLLCVACFASDSGYFVFG
                     IICLNLLFARLAGEDALKRSLRMLKALAKFSLFLFIIQILCVRSGTVLLELPFQLVIT
                     DEGLRFSLLFILRLFAATMPIGLMLSVTTMGDLSNVLVEKLHVPYKYAFALTTAIRFI
                     PIFSQEMSGIMEAQTARGVEFDTKNFFKKVKLLLPLCVPLLLSSVRKIDSAAIAAELR
                     GFHCRK"
     gene            complement(675..>1121)
                     /locus_tag="PLU43_12195"
     CDS             complement(675..>1121)
                     /locus_tag="PLU43_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOO29217.1"
                     /translation="RFQFLPDADPFRLSRGDRQRLALASILACHPEFLLLDEPTTGLD
                     YRECMQLLTMMQELHQKGTTILMITHDMEIVQEFAQRVLVMTNGRLVADGATGDVMRE
                     TKTLKQASVMPSQMQELALELGDSYDGVYSVETMLARIDCLRRRRA"
BASE COUNT          323 a          303 c          232 g          263 t
ORIGIN      
        1 ctttctgcag tgaaatccgc gcagttccgc tgcaatagca gccgagtcta tcttcctgac
       61 cgaacttaac aaaagcggta cacaaagcgg aaggagcagt ttgacctttt taaaaaaatt
      121 ctttgtatcg aattccacgc ctctggctgt ctgtgcttcc atgattccgg acatttcctg
      181 cgaaaagatc ggtataaaac ggatcgcggt cgtcaatgca aacgcatatt tatacggaac
      241 atgcagtttt tccacaagca catttgacag atcacccatc gtagttacgg acagcatcaa
      301 accgatcggc atcgtagccg caaataaacg cagaataaac aataaagaaa atctcaatcc
      361 ttcatccgta ataacgagct ggaatggaag ctccaaaagt accgttccgc tccttacgca
      421 aaggatctgg attatgaaca gaaaaagcga aaattttgca agcgctttca gcatccgtaa
      481 agatcgtttc aaagcatcct ctcctgcaag ccgtgcaaag agcagattca aacagatgat
      541 cccaaataca aaatagccgc tgtcactcgc aaaacaggca acacaaagca aaaaagccgc
      601 aagaagcttt gtcagcggat ttagtttatg aagcagcgaa tttcccggta tataatctaa
      661 aaatccaccc atttttatgc cctccttctt cgcagacagt caatacgggc aagcatcgtt
      721 tccacggaat acacaccgtc atagctgtcg ccgagctcca gtgcaagctc ctgcatctgt
      781 gaaggcatga cacttgcctg cttcagagtt tttgtctctc tcatcacatc accggtcgct
      841 ccgtccgcca ccagtcttcc gtttgtcata acaagtacgc gctgtgcaaa ctcctgcacg
      901 atctccatat catgcgtgat cattaaaatc gtcgttccct tttggtgcag ctcctgcatc
      961 atcgtaagca gctgcataca ctcccggtag tcgagtccgg tcgtcggttc atcaagcagc
     1021 agaaactccg ggtggcaggc aaggatcgaa gccagtgcca aacgctggcg gtcacctctg
     1081 cttagacgaa acggatccgc atccggcagg aattgaaacc g
//
LOCUS       DAOSYE010000865         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_123853_length_1120_cov_3.577465, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000865 DAOSYE010000000
VERSION     DAOSYE010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123853_length_1120_cov_3.577465"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1056
                     /locus_tag="PLU43_12200"
     CDS             262..1056
                     /locus_tag="PLU43_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29218.1"
                     /translation="MDGFRERFSGRFDRSTERGGSDRNNQDRYERTARKAPSSGYGAS
                     ERLGLEEITEAIDKSNTKQLEIIGDFFDEAKDDRLTSEKDILQALDENAGLVDKNTSL
                     LQQNAELIRQNADVLNDIRIKALDRAQAPAPVPAPPVQIPSVDGAAKEEIMQALLSNT
                     SLLNMMRQEIAVYRTEPEPEKPAEDESQAVITAIDNYFKNMEDHVHKENVKCYRNVQA
                     AVTEQNVQTVDETKKNLGALKVFAIVSMVLSVVNIGLLAAYIFGVI"
BASE COUNT          380 a          185 c          279 g          276 t
ORIGIN      
        1 aggtggatga gaatcacctg ggcaatatta tgaaaaaatt tatctgataa ccaatgttat
       61 ttcttttctt tgaactataa atagtgtagc aataatatat gaataaatta tgaacaacat
      121 gtaaaagaat agttaatatt tacaaaaaac aaaataaata aattcgtgaa ttatacatgt
      181 tgcacaaaac gcgaaaccct tgtaattaag cggaaataat gataaaatat aaaaaaagtt
      241 tcgtacggag cggaggtaca tatggacgga ttcagggaaa gatttagcgg cagattcgat
      301 cgttccacag agcggggcgg ttctgacaga aataatcagg atagatatga gagaactgca
      361 aggaaagctc catcatcggg ttatggcgca tccgaaaggc ttggccttga ggagattacg
      421 gaagcaatcg ataaaagcaa cacaaagcaa ctggaaatca taggagattt ttttgacgag
      481 gcaaaggatg atcgtctgac atcggaaaag gatattttac aggcacttga tgaaaatgcg
      541 ggactggtag ataagaatac gtcgctgctt cagcagaatg cggaactgat ccgtcagaat
      601 gcggatgttc tgaatgatat tcggataaaa gcactggatc gggctcaggc acctgcgcct
      661 gttccggcac ctccggttca gataccgtct gttgacggag cggcaaaaga agagatcatg
      721 caggcattgc tcagcaatac gtcgcttttg aatatgatgc gtcaggaaat tgccgtatac
      781 aggacagaac cggaaccgga aaagcctgcg gaggatgaat cacaggcagt gatcacggcg
      841 atcgataatt attttaaaaa catggaagat catgtccata aagaaaatgt gaaatgttat
      901 cggaacgtac aggcggccgt tacggaacag aatgtacaga cagttgatga gacaaaaaag
      961 aatcttggtg cgttaaaggt attcgcgatc gtttccatgg tattatcggt cgtaaatatc
     1021 ggacttcttg cggcttatat tttcggagtt atataatggg aaagatcgaa tggaagccgg
     1081 ggaatatgct gtatccatta ccggcggtat tggtcagtgt
//
LOCUS       DAOSYE010000866         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_123867_length_1120_cov_3.064789, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000866 DAOSYE010000000
VERSION     DAOSYE010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123867_length_1120_cov_3.064789"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLU43_12205"
     CDS             complement(<1..484)
                     /locus_tag="PLU43_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29219.1"
                     /translation="MRIVVNDIAASKTGALSVLKDFYHYIAEYDTENEWFFLLGGNYV
                     EETKRIHVCVLPDVKKSRFSRLKFDFFTGAGYLRSLKPDIVFSLQNTLTYGYHGKQAV
                     YVHQPLGFQKVKKFSFFDPKEREYAVYQYVIGAMIDSAVRRSDLTIVQTEWMRRAVVQ
                     K"
     gene            complement(481..>1120)
                     /locus_tag="PLU43_12210"
     CDS             complement(481..>1120)
                     /locus_tag="PLU43_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOO29220.1"
                     /translation="TIFVTGSPMREVLRDHMGKIQESDVLYRLGLEKRKYFLVSAHRE
                     ENIDMEENFMDLMNSINNIAEKYQMPVIYSTHPRSKKFIEMRNFKFHPLVTNMKPFGF
                     LDYNKLQMNAYCVLSDSGTLSEESAMLRFPGVLIRTSTERPEVLDKGTVVIGGIKGKD
                     VEQATALAIAMYENKEEVIMAEDYADTNVSVKVVKLIQSYTKIVNQTVWCKA"
BASE COUNT          298 a          292 c          198 g          332 t
ORIGIN      
        1 tcttttgaac gactgctctt ctcatccact ccgtctgtac gatcgtaagg tccgaccgcc
       61 tgaccgcact gtcgatcatt gcgccgatca catattgata aacagcatat tcccgctctt
      121 ttggatcaaa aaaagaaaat ttctttacct tctgaaagcc gagcggctga tgcacataca
      181 cggcctgctt tccatgatac ccatatgtca gcgtattctg cagcgaaaat acaatatccg
      241 gctttaggga gcgcaaatat ccggctccgg taaaaaaatc aaatttcagc ctggaaaacc
      301 ggcttttctt cacatccggc aatacgcaca catgaatgcg cttcgtttcc tccacataat
      361 tgccgcccag taaaaaaaac cattcgtttt ccgtatcata ctctgcaata taatgataaa
      421 aatcctttaa aaccgaaagc gcgcctgttt tgctcgccgc aatatcattt actacgattc
      481 tcatgctttg caccagactg tctgattgac gattttcgtg taactctgta tcagctttac
      541 gactttaacg gaaacgtttg tgtccgcata gtcttctgcc atgatcactt cctctttatt
      601 ttcatacatg gcaattgcaa gcgcggtcgc ctgctccaca tctttgcctt tgataccgcc
      661 gatcacgacc gttcccttgt cgagcacctc aggacgttct gtcgaagttc tgatcagcac
      721 ccctggaaaa cgaagcatcg cagactcttc ggacagtgtc ccgctgtccg aaagtacaca
      781 gtatgcattc atctgcagct tattatagtc aagaaaacca aatggtttca tattcgttac
      841 aagcggatgg aacttaaaat ttctcatttc aataaatttt ttcgaacgcg gatgtgtcga
      901 ataaatgacc ggcatctgat acttttccgc aatattgtta atggaattca tcagatccat
      961 aaaattctct tccatatcga tattttcttc cctgtgtgcc gatacaagaa aatattttcg
     1021 cttttccaga ccaagcctgt acaaaacatc gctttcctgt atttttccca tatggtcacg
     1081 cagcacttca cgcatggggg agcctgttac aaagatcgtt
//
LOCUS       DAOSYE010000867         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_123917_length_1120_cov_2.509859, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000867 DAOSYE010000000
VERSION     DAOSYE010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123917_length_1120_cov_2.509859"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLU43_12215"
     CDS             <1..271
                     /locus_tag="PLU43_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29221.1"
                     /translation="TLLHEMTGLSFLDMEEKPDLYYSSLYDAYYMKKSDTGCTKIKCT
                     AGTVDSTGLYSIFYTKNDESGLWNVVLKAYDGRKLFYSNTDTVRK"
     gene            453..869
                     /locus_tag="PLU43_12220"
     CDS             453..869
                     /locus_tag="PLU43_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29222.1"
                     /translation="MEEKTTADNGRAWKMVLFIFVCIFAVPVIIPVGIALGITILALI
                     ISVAAVLISLVIGFGAAAFGCLAAGLAAVVYGFISLFHVPIAGFVVLGAGLLVLAIGL
                     LLLVLTVLIAGKLIPMFFRGTVKVCGAPFRRKEIVV"
     gene            866..>1120
                     /locus_tag="PLU43_12225"
     CDS             866..>1120
                     /locus_tag="PLU43_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29223.1"
                     /translation="MNRFIKECLTVASGLCAIGLVLVIIGGIFGGFRQTAAIALNGGF
                     VYPIYLYDTMEESTDNESSSDFQEMSDGFYENDDVCDMSDV"
BASE COUNT          279 a          201 c          304 g          336 t
ORIGIN      
        1 tacgctttta catgaaatga cgggacttag ttttttggat atggaggaga agccggacct
       61 gtattacagc agtctctatg acgcctatta tatgaaaaag agcgataccg gatgtactaa
      121 aatcaaatgc acggcaggta ctgtcgattc gacaggattg tacagtattt tttatacaaa
      181 aaatgatgaa agcggtctgt ggaatgttgt tctgaaagca tacgacggcc ggaaactgtt
      241 ttactctaac acagacactg tccgtaagta aaaataacaa aaataatact tcatttcctt
      301 ctgtcacaac cttgcgtgac aattgtcacg accttcccaa aacctattta cagcctgatt
      361 tttatatagt aagcttcatc ttgaacaggg tgagttttta tattgcacgg caggagaatc
      421 ggccgggata acggaaaggc aggaagggtt ctatggagga aaaaacaact gcggataacg
      481 gcagggcgtg gaagatggtt ctttttattt ttgtttgtat ttttgcggta ccggtcatta
      541 taccggttgg aattgcgctg gggatcacga ttctggcatt gatcatatcg gtggcggcgg
      601 tgctgatatc cctggtgatc ggatttggcg cggcggcgtt tggatgtctg gcggcaggac
      661 ttgctgcggt agtttacgga tttatcagtc tgtttcatgt accgattgcc ggatttgttg
      721 ttttgggagc gggtcttctg gttctggcaa tcggtctttt gctgcttgta ctgaccgtat
      781 tgattgccgg taagctgata ccgatgtttt tcaggggaac tgtgaaagtg tgcggagctc
      841 cgtttcggag aaaggagatc gtcgtatgaa ccgatttata aaagaatgtc tgacagtggc
      901 ttccggtctg tgcgcgatcg gacttgtgct tgtgatcatc ggcggaattt tcggaggatt
      961 cagacagaca gcagccattg cactgaacgg cgggttcgta tatccgatct atctgtatga
     1021 tacaatggag gaaagcaccg acaatgagtc ttcttccgat tttcaggaaa tgtcggatgg
     1081 tttttatgag aatgatgatg tctgtgatat gtcggatgtg
//
LOCUS       DAOSYE010000868         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_124079_length_1119_cov_3.196429, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000868 DAOSYE010000000
VERSION     DAOSYE010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124079_length_1119_cov_3.196429"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLU43_12230"
     CDS             <1..611
                     /locus_tag="PLU43_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29224.1"
                     /translation="IGIYSPVTNVLKTSFAMTLGQILAEEEEVLFISLECCSGLTGLL
                     SLNKERDLSDLLYERSVGEARFSLGRFTQRAEGLSIISPVDGVTELQSVKKEEWVKLM
                     RFLLREGSYSKLILDIGNSVNGIIEILQLCGMIYMPYRQDRISMAKLSAFETCIDRYE
                     AAQDLKKKIKKLKMPYFENLEESLVELKYGAFGSFVRTILSK"
     gene            627..>1119
                     /locus_tag="PLU43_12235"
     CDS             627..>1119
                     /locus_tag="PLU43_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO29225.1"
                     /translation="MPEYFEDRKREIKEDVLQQLSERVETSEEEVRSVIDECILRHSR
                     SHCLDLAQKCRIRRELFNAIKRLDVLQELIEDETVTEIMVNGIQNIFIEKDGKLEDSG
                     LRFESKERLMDIIQQIAAGVNRTVNVSSPIVDARLPDGARVNAVLEPAAINGPILTIR
                     RFPK"
BASE COUNT          335 a          193 c          310 g          281 t
ORIGIN      
        1 tgatcggaat ttattcaccg gtaacaaatg tattaaaaac atcgtttgca atgacacttg
       61 gtcagatcct tgcggaagag gaagaagtgc tgttcatcag cctggaatgc tgttccggtc
      121 ttaccgggct gctttcgctt aataaggagc gtgatctgtc tgatctttta tatgaaagat
      181 ctgttgggga agcccggttt tcattaggcc ggtttacaca aagggcagag ggactttcga
      241 tcatctcccc ggtggatgga gttacagagc tgcaaagcgt caaaaaagag gagtgggtaa
      301 agctgatgcg ctttcttttg agggaaggca gctattccaa attgattttg gatattggaa
      361 acagtgtaaa tggaattata gaaatactgc agctgtgcgg tatgatctat atgccttacc
      421 ggcaggaccg gatttcaatg gcaaagcttt cggcatttga aacctgcatt gacagatatg
      481 aagcggcaca ggaccttaaa aaaaagataa aaaaactgaa gatgccttat tttgaaaatt
      541 tagaagagag tcttgtggag ttaaaatacg gcgcatttgg cagttttgta agaacgatct
      601 taagtaagta aaaggacagg agtggtatgc cggaatattt tgaggataga aaaagggaaa
      661 taaaagagga tgtactgcag cagctgtcgg aaagggtgga aacgtctgaa gaagaggtcc
      721 gcagtgtgat cgatgaatgt attttgcgcc acagccgcag ccattgtctg gatcttgcac
      781 agaaatgccg gatacgccgg gagttattta atgcaatcaa gcgtctggat gtgctgcagg
      841 aattgataga ggatgagaca gttacggaaa tcatggtgaa tggaatacag aatattttta
      901 ttgaaaaaga cggaaaactg gaagacagcg gcctgcggtt tgaatcaaaa gaacgtttga
      961 tggatattat tcagcagatc gcggcagggg tcaaccgtac ggtcaatgtg tcctcgccga
     1021 tcgtagatgc cagactgccg gatggagcgc gtgtcaatgc ggtgcttgag ccggcagcaa
     1081 tcaacggccc gattctgacg atcagacgat ttccaaagg
//
LOCUS       DAOSYE010000869         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_124420_length_1117_cov_3.513183, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000869 DAOSYE010000000
VERSION     DAOSYE010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124420_length_1117_cov_3.513183"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="PLU43_12240"
     CDS             complement(<1..>1117)
                     /locus_tag="PLU43_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOO29226.1"
                     /translation="LIIVIASVISVFCLTLSFFISLSITRPVKRVLQLIQKTSALDLT
                     YDASYDKLFQYKDEVGDIARAIDSLRTSLRNFSANLLTISQELDNSSKELEESAGENS
                     KSIEQIATAINEIAQGNSSQANEITKTSDSAIAITDHVREINEVSNESAKYAEQSRTL
                     ITDGQAAIGLTVEKLSENIKVSDEVNTSISELSEQMVKVGEIVDVIRQISSQTNLLAL
                     NASIEAARAGEAGRGFAVVATEIGNLAQNTSSAVDDIANIIDTAVKKSNETSDRIGEV
                     RRIVGEQENTVHVTEESFTNIEHSVDKITTLALDISQKVSAVVKQSENVSEHMQDMSA
                     VAEETAAGSQEISASSEEQLSSMESIAKITSQLSEMAG"
BASE COUNT          268 a          261 c          253 g          335 t
ORIGIN      
        1 ttccggccat ttcagaaagc tgggaagtga ttttcgcaat agattccata gaggacagct
       61 gttcttcact tgaagcggat atttcctgtg agccggcagc tgtttcttct gcaaccgcag
      121 acatatcctg catgtgctcg gaaacgtttt ccgactgctt cacaacagcc gaaacctttt
      181 gggaaatatc aagtgccaga gttgtgattt tgtcaacaga atgctcaata ttggtaaagg
      241 attcttccgt cacatgcacc gtattttcct gttcgcctac gatccgccgc acttcgccga
      301 tccggtctga tgtttcattg ctctttttta cggctgtgtc aataatattt gcgatatcat
      361 caactgcgga ggaagtattc tgtgcgagat ttccgatctc agtagcaaca actgcaaaac
      421 cgcgtcctgc ttcgccggct ctggctgctt caatggaagc attcagtgca agcaggtttg
      481 tctgggagga aatctgccgg atgacatcta cgatctcccc aaccttgacc atctgttcac
      541 tcaattcgga aatgcttgta ttgacttcgt cggaaacttt aatattttcg ctgagttttt
      601 cgaccgttaa tccgatagct gcctgtccgt ccgtgatcag ggtccgcgac tgttccgcat
      661 acttggcact ttcattgctt acttcattga tctctcttac atgatcggta attgcgatcg
      721 cagaatcgct ggtctttgta atttcgtttg cctgggaaga atttccctgt gcgatctcgt
      781 tgatcgcggt tgcgatctgt tcgattgact tgctgttttc gccagcagat tcttccagtt
      841 ctttggaact gttgtccagc tcctgagaaa tcgtcagcag atttgcggag aaattgcgca
      901 gtgaagtgcg cagactgtcg atcgcccgtg cgatatcccc aacctcatct ttatattgaa
      961 ataatttgtc ataagaagca tcatatgtga gatccagcgc agaagttttc tgaatcagct
     1021 gaaggacccg ttttacggga cgtgttatag aaagcgatat aaagaatgac agagtcagac
     1081 aaaaaacaga aataacggag gcaatcacga tgattaa
//
LOCUS       DAOSYE010000870         1115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_124801_length_1115_cov_3.574528, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000870 DAOSYE010000000
VERSION     DAOSYE010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124801_length_1115_cov_3.574528"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1115
                     /locus_tag="PLU43_12245"
     CDS             2..>1115
                     /locus_tag="PLU43_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO29227.1"
                     /translation="MEKNRIRPVTVGKSTRMSYSRQKEVLEMPNLIEVQKDSYQWFLD
                     EGLKEVFADISPITDYSDHLSLEFVDFVLCEDEVKYSIEECKERDATYAAPLKVRVRL
                     YNKQDDKIIEHEIFMGDLPLMTATGTFVINGAERVIVSQLVRSPGIYYAISHDKLGKR
                     LFSSTVIPNRGAWLEYETDSNDVFFVRVDRTRKVPVTVLIRSLGIGTNQEILELFGDE
                     PKILASFTKDTAENYQEGLLELYKKIRPGEPLTVESAESLINAMFFDPRRYDLAKVGR
                     YKFNKKLALRNRIRHQILAEDVVDMTTGEILAKQGTKVTMELADLIQNAAVPFVKEFA
                     KHPAFFAVDIMNEADGAIGGIDFLSGGMNPKLGCTWK"
BASE COUNT          322 a          196 c          280 g          317 t
ORIGIN      
        1 aatggaaaaa aacagaatac gtccggtcac agttggtaag agtacacgta tgagttattc
       61 acgacaaaaa gaggtattgg aaatgccgaa tctcattgag gtacagaaag attcttacca
      121 gtggtttttg gatgagggtc tgaaagaagt ctttgcggat atctcgccaa ttacggatta
      181 cagtgaccat ttgagtcttg aattcgtaga ttttgtattg tgtgaagatg aagtaaagta
      241 ttctattgaa gagtgcaaag aacgagatgc aacttatgca gctcccctga aagtcagggt
      301 cagactttac aataaacagg atgataagat cattgagcat gaaatattta tgggcgatct
      361 tccgttaatg actgcgacag gtacttttgt tatcaatggt gcggagcgtg ttattgtcag
      421 ccagcttgtt cgttcaccgg gtatttatta tgcgatcagt catgataagc tcggaaagag
      481 gttgttttct tcgacagtta tccccaatag aggcgcatgg ttggaatatg aaacagattc
      541 caatgatgtt ttctttgtgc gtgtagacag gacaagaaaa gttcccgtta cggtattgat
      601 ccgttctctc ggaatcggta ccaatcagga aattcttgaa ttgtttggcg acgaacccaa
      661 gatccttgct tcctttacaa aggatacggc tgaaaactat caggaaggtc ttttggaatt
      721 atataagaag atccgtccag gtgagccgct taccgtggaa agtgcggaaa gcttgattaa
      781 tgcgatgttt tttgacccca gaagatacga tctagccaaa gtgggcagat ataaatttaa
      841 taaaaagctg gctctgcgca atcggatcag acatcagatc cttgcggagg atgttgttga
      901 catgacgact ggtgagatcc ttgcaaagca gggtacgaaa gtaacaatgg agcttgctga
      961 tctgatccag aatgcggccg ttccttttgt taaagagttt gcaaaacacc ccgccttttt
     1021 tgccgtcgat atcatgaatg aggcagatgg tgcgattggc ggaattgatt ttttgagcgg
     1081 tggtatgaat cctaaacttg gctgcacctg gaagc
//
LOCUS       DAOSYE010000871         1114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_124963_length_1114_cov_3.512748, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000871 DAOSYE010000000
VERSION     DAOSYE010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124963_length_1114_cov_3.512748"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1114
                     /locus_tag="PLU43_12250"
     CDS             <1..>1114
                     /locus_tag="PLU43_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOO29228.1"
                     /translation="VTLTTTGAIGISAMMHGYMPFDAAGNLLVPFRTWRNNITGEASE
                     QLSALFQFHIPQRWSIAHLYQAILNNEPHVKDIAFITTLESYVHYMLTGKRVLGIGDI
                     AGMFPVDAKTKDYNQRMIDQFEELVAPYAFSWKFREIFPKAMLAGQNAGTLTEAGAKF
                     LDPSGDLKAGIMLCPPEGDAGTGMVATNSVRQRTGNISAGTSVFAMVVLENDLTKPYE
                     EIDMVTTPAGDPVAMVHCNNCTSDLNAWVNLFKEFAENFDIDVDMTKLFSVLYNKALE
                     GDPACGGMLAYNYFSGEHITKFTEGRPLFVRTPNSKFNLANFMRLHLFTSLGALKTGL
                     DILFKSEQVTIDKILGHGGLFKTKGVGQKIVAGAINV"
BASE COUNT          296 a          228 c          292 g          298 t
ORIGIN      
        1 atgtaacact tacaacgact ggtgcgatcg gcatcagcgc aatgatgcac ggctatatgc
       61 cgtttgatgc ggctggaaat ctgcttgtgc cgtttcggac atggcggaat aatattacgg
      121 gagaggcatc ggaacagctg tccgcacttt tccagttcca tattccgcag cgctggagta
      181 ttgctcatct gtaccaggca attttgaata atgaaccgca tgtaaaagat attgctttta
      241 ttacgacact ggagtcttat gttcattata tgctgacagg gaaaagggtg cttggtatcg
      301 gagatattgc cggtatgttc ccggtggacg caaagacaaa ggactataat cagcggatga
      361 tcgatcagtt tgaagagctg gtcgcaccct atgcattctc atggaaattc cgtgagatct
      421 ttccaaaggc aatgcttgcc ggacagaatg ccggaactct gactgaggcc ggtgcaaagt
      481 ttttggatcc ttcgggagat ctgaaagcag gcattatgct ctgtccgccg gaaggcgatg
      541 caggaaccgg tatggtggct actaattccg tcagacagcg tacgggaaat atttcggcag
      601 gcacatccgt atttgcaatg gtcgtgcttg aaaatgacct gacaaaaccg tatgaagaga
      661 tcgatatggt cacaacgccg gcaggtgatc cggtcgcaat ggtacattgc aacaactgta
      721 cgtccgattt gaatgcatgg gtcaatctgt ttaaggaatt tgccgagaat tttgatattg
      781 atgtggatat gacaaaactg ttttccgttc tttataacaa ggcgctcgaa ggagatcccg
      841 catgcggcgg tatgctggcc tataattatt tttctgggga gcatattaca aagtttaccg
      901 aaggccgtcc gctgtttgtc agaacgccga acagcaagtt caatcttgcg aattttatga
      961 ggctgcatct gtttacatcc cttggagctt taaaaacagg gctggatatt ctttttaagt
     1021 cggaacaggt tacgatcgat aaaatacttg gacacggcgg tctgtttaaa acaaagggcg
     1081 tcggacagaa aattgtggca ggagccatca atgt
//
LOCUS       DAOSYE010000872         1114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_125078_length_1114_cov_1.742210, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000872 DAOSYE010000000
VERSION     DAOSYE010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125078_length_1114_cov_1.742210"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLU43_12255"
     CDS             <1..920
                     /locus_tag="PLU43_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29229.1"
                     /translation="DENDFYMHLGHALLDVKMKKYERQIKIYYHSRIDAAYADTHRQA
                     VEAAGREVADVFDDYEKTCLEMGQKKYAEYRNHLPPDADDINELLIRGEEQSKVLPMP
                     EKRYQDIKLTESVCMLQSPGKSVNELLADALDLFIAGDYQKAFDIYCRLREELTEDTD
                     HYSWVQFQTGKLFCIFDDNQKALEALNCCRPERFGLVYRQEDFFLLYEHVKIVCDDFE
                     SDVRYRKLIRGKFDFYYAQYDREYNQMLRDRKLMHEFGQYERDCMENAAAAFLSEHGI
                     ETDCQEEASSGGREPLFFKNMFKFFKENK"
BASE COUNT          353 a          195 c          287 g          279 t
ORIGIN      
        1 aggatgagaa cgacttttac atgcatcttg gacatgcact tttggatgta aagatgaaaa
       61 aatatgagcg tcagatcaaa atctactacc acagcaggat cgacgcggcg tacgccgata
      121 cgcacaggca ggcagtagaa gctgccggca gggaagtagc cgatgtattt gatgactatg
      181 aaaaaacgtg tcttgaaatg ggacagaaaa aatatgccga atatcgaaat catctgccgc
      241 cggatgcgga tgatatcaat gaattgctta tcaggggaga ggagcagagc aaggtgctcc
      301 caatgccgga aaagcgctat caggatatta agctgacaga atcggtctgc atgctgcaat
      361 cgccggggaa gtcggtaaac gagctgcttg cggatgccct ggaccttttt attgccggcg
      421 attatcagaa ggcatttgat atttattgca gactgcggga agaactgaca gaagatacgg
      481 accattattc atgggtacag tttcagaccg ggaaattgtt ctgcatattt gatgacaacc
      541 aaaaggcgct tgaagcattg aactgctgca gaccggaacg atttggactt gtataccggc
      601 aggaggattt tttccttctt tatgagcatg taaagatcgt ctgtgatgat tttgaaagtg
      661 atgtcagata ccgcaagctg atacgcggga aatttgattt ttattatgcc cagtatgaca
      721 gggaatataa tcagatgctg cgggacagaa agctgatgca tgaattcggc cagtatgaac
      781 gggattgtat ggaaaatgct gccgcagcat ttttatctga acatggaatt gagacggatt
      841 gccaggagga ggcatcttcc ggcggaagag aaccgttatt ttttaaaaat atgttcaaat
      901 tttttaaaga aaataaataa ggcattttag ttattgcaga cgccgcacga caggaaagaa
      961 acagttctgt cgatgcggtg tttttttgta gggcagtttt tattgacacc aaaaaactga
     1021 gacgatataa taaatgaaca accgattgcg caaataaaca gaaacggagt tccggtatgg
     1081 atgaaaagaa aaatctgacg atcaatgata tagc
//
LOCUS       DAOSYE010000873         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_125749_length_1110_cov_3.053081, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000873 DAOSYE010000000
VERSION     DAOSYE010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125749_length_1110_cov_3.053081"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLU43_12260"
     CDS             <1..214
                     /locus_tag="PLU43_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29230.1"
                     /translation="GVIDHKRLYFKDAPIINSFMCGSIKYRPHFQMALSSFKNRITFS
                     SNLYGSRKDRKNILHFYSLLDRELDF"
     gene            286..471
                     /locus_tag="PLU43_12265"
     CDS             286..471
                     /locus_tag="PLU43_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29231.1"
                     /translation="MLSFPCRWKYDILRCMDYFASVQKEYDPRMEEALERIIRKKRRN
                     DRWPVQQKYTGLRYTGK"
     gene            678..>1110
                     /locus_tag="PLU43_12270"
     CDS             678..>1110
                     /locus_tag="PLU43_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HOO29232.1"
                     /translation="MQQIAKFYKVSYNQFEKDWLDSFGSTDEEIAEIYADIHLPKRAT
                     TGSAGYDFFSPISFNLKAGETIKIPTGIRVEMENGWVLQCYPRSGLGFKYRLQLNNTV
                     GIIDSDYFYSDNEGHIFSKITNDSNEGREVNVEAGMGFMQGI"
BASE COUNT          333 a          176 c          271 g          330 t
ORIGIN      
        1 cggagttatc gatcataaaa ggctgtattt taaggatgca ccaattataa attcatttat
       61 gtgcgggtca ataaaataca ggccgcattt tcagatggct ttgagcagtt ttaagaacag
      121 gataaccttc agcagtaatt tgtatggaag cagaaaggat cgtaaaaata ttttgcattt
      181 ttacagcctg ttagacaggg agcttgattt ttgaattttt aatgaccgga ccgtgcagtt
      241 ttttgcaatg aatgcaaggc agatttcatt gacaaaagga tgctgatgct gtcttttccg
      301 tgccgctgga agtatgatat tctgcggtgt atggattatt ttgcgtccgt acagaaagag
      361 tatgatcccc gcatggagga agcacttgag cgtattatcc gaaagaaaag gagaaatgac
      421 cgctggccgg ttcagcagaa gtatacaggc ctgcgctata cagggaaatg atttataaat
      481 aataaaggaa aatgagtatg cgtcggaagc caggcagagt tctgcctggc tgtttccatg
      541 tacatagatg ctgttatctg cggcaggttt ttcgactata caagaaaaac attttttggt
      601 atactatttc tggtagcgtt atttaccata tcacaattaa tattgtcgcc tgtgcagata
      661 aaggagtaga tcggaatatg cagcagatag caaaatttta caaagtaagt tataatcaat
      721 ttgagaaaga ctggcttgac agctttggat caacagacga ggaaatagct gaaatctatg
      781 cggatatcca tcttccgaag agggcgacga ccggttcggc gggatatgat tttttcagtc
      841 cgatttcttt taatctgaaa gcgggtgaga ccattaagat tccgaccggt attcgtgtgg
      901 aaatggaaaa cggctgggtc ctgcagtgtt atccgagaag cggtcttggc tttaaatatc
      961 gtctgcagct gaataataca gttggtatta ttgacagtga ttatttttat tccgataatg
     1021 aaggtcatat tttttcgaaa atcacaaatg attccaatga aggaagggaa gtaaatgtgg
     1081 aagccggtat gggtttcatg cagggtattt
//
LOCUS       DAOSYE010000874         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_125752_length_1110_cov_3.042654, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000874 DAOSYE010000000
VERSION     DAOSYE010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125752_length_1110_cov_3.042654"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1110
                     /locus_tag="PLU43_12275"
     CDS             <1..>1110
                     /locus_tag="PLU43_12275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOO29233.1"
                     /translation="LNEPVTVSRTMYWDVLRLFHEMKQGIIKAKLNGGFDSIAVDTWG
                     VDFGLIDENGNLLENPVHYRDARTEGMFEKAFSRFPKDEFYEITGNQFMEINTAFQLM
                     ALLEKRPEILKRADKLLLMPDLFNYFLTGIKSAEYSIASTTQLMDARNRKWSGKVMDS
                     LGIPRRIFPDIISSGTKLGAVSPEICAELGLDFVPEVVAIAGHDTQSALVSIPAAEED
                     FIFISCGTWSLFGTELAEPLINEESARLNITNEGGYGGRISFLKNIIGLWLIQESRRQ
                     WAREGKEYGFGELETMAKAAKPFQCFIDPDAPEFVPSGNIPERIREYCRRTDQKVPET
                     EGELVRCIDESLAFKYRDTFQEIRECTGKEYQTIYL"
BASE COUNT          315 a          204 c          320 g          271 t
ORIGIN      
        1 cttaaatgaa ccggtgaccg tcagccggac aatgtattgg gatgtgctcc gtctgtttca
       61 tgagatgaaa caggggatca taaaggcaaa actgaacggc ggatttgaca gtatagcagt
      121 tgatacatgg ggagtggatt tcggtttgat cgatgaaaac gggaatctgc ttgaaaatcc
      181 ggtgcattac agggatgcaa gaacggaagg aatgtttgaa aaggcatttt ccagatttcc
      241 gaaagacgag ttttatgaaa ttacgggcaa tcagttcatg gagatcaata cggcatttca
      301 gcttatggca cttttggaaa agaggccgga aatactgaag cgggccgata agcttttgct
      361 gatgccggat ttattcaatt attttctgac agggatcaaa agtgcggaat attcgattgc
      421 atcgacaaca cagctgatgg atgccaggaa tcgtaaatgg tccggtaagg tcatggattc
      481 gctcggtatt ccgaggcgaa tttttccgga tattatttca agcggtacaa agcttggagc
      541 agtgtcgccg gagatctgcg cggaacttgg cctggatttt gtaccggagg tagtggcaat
      601 tgccggtcat gatacgcaga gcgcacttgt cagtattccg gctgcggagg aagattttat
      661 ttttatcagc tgcggaacgt ggtcactgtt tggtacggag ctggcggagc cgctgatcaa
      721 tgaagaatcg gcgcgtttaa atattacaaa cgaaggcggt tacggcggaa ggatctcctt
      781 tttaaaaaat attatcgggt tatggctgat tcaggaaagc cgcaggcaat gggcaagaga
      841 aggaaaggaa tacggatttg gcgagcttga gacgatggcg aaggctgcaa agccgtttca
      901 gtgctttatt gatccggacg caccggagtt tgtgccaagc ggaaacattc cggaaaggat
      961 ccgggaatac tgccggagaa cagaccagaa agtacctgaa acagagggag agctcgtccg
     1021 ctgtattgat gaaagccttg cctttaaata ccgggacacg tttcaggaga tcagagaatg
     1081 taccggaaaa gaatatcaga cgatctatct
//
LOCUS       DAOSYE010000875         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_125939_length_1109_cov_3.288425, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000875 DAOSYE010000000
VERSION     DAOSYE010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125939_length_1109_cov_3.288425"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLU43_12280"
     CDS             <1..192
                     /locus_tag="PLU43_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOO29234.1"
                     /translation="LIVTITSLCLTLKTNIINVFTGAEGMGWSIIRIVVGGLLIILAV
                     ILAVDGIKTLTKQEKKVKA"
     gene            388..1017
                     /locus_tag="PLU43_12285"
     CDS             388..1017
                     /locus_tag="PLU43_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOO29235.1"
                     /translation="MNTIVTIGREFGSGGREIGEKLAAKLGIKCYDKELLKRAAKDSG
                     MCPELFENHDERPTNSFLYNLVMDTYTFGYSASTYVDMPISHKVFLAQFDTIKKIAAE
                     GPCIIVGRCADYALADYKNCIHLFIHGELEKRIQRIQEKYDLTYDKAKEMINKKDKQR
                     SNYYNYYSSKKWGLSESYHMSIDSSVLGIDGTVELIMQFIQDFEKGQES"
BASE COUNT          370 a          173 c          218 g          348 t
ORIGIN      
        1 ctgattgtaa ccattacatc tttatgcctg acactgaaaa ccaatattat caatgtattt
       61 acaggcgcag aaggaatggg atggagcatt atccgtatcg ttgtcggcgg attattgatt
      121 attcttgctg ttattcttgc agtcgatggt attaaaacat tgacaaagca agaaaaaaaa
      181 gtaaaagcat aggtattata taaaacaaaa tacctgttcg catatgttca ttcaaacagt
      241 tccatttttt gaatgcccgc ggacaggtgt tttatttttt tattccaaat catcgcaaat
      301 tgttgtataa ttattatata gaaaccatat atagtatgtt tttatttgat cgaaaataaa
      361 aaaagaaatg gaaaagaggt gttttttatg aatacaatcg ttacaattgg ccgggaattc
      421 ggcagcggcg gaagagagat cggggaaaag ctggcagcaa aacttggcat caaatgctat
      481 gataaagagc tgctgaaacg tgctgcaaag gacagcggca tgtgtccgga attatttgaa
      541 aatcatgacg aacgtccgac aaacagtttt ttatacaatt tggtcatgga cacttatacg
      601 ttcggatatt ctgcttctac ttatgtagat atgccgatca gccataaagt atttttagca
      661 cagtttgata caattaaaaa aatcgctgca gagggtccat gcatcattgt cggccgatgt
      721 gcggactatg cactggcaga ttataaaaac tgtattcatt tatttataca cggcgaactg
      781 gaaaaacgta ttcagcgcat tcaggaaaaa tatgatctga cttatgataa agcaaaggaa
      841 atgatcaata aaaaagataa acagcgttcc aattattata attattattc ctcaaaaaaa
      901 tggggattat ctgaaagtta tcacatgtct atcgacagca gtgttcttgg catcgacggt
      961 accgtagagc ttattatgca gtttatacag gattttgaaa agggacagga atcttagatt
     1021 atgaaatatc ctattgacaa aaaattcatt cattggggtc tgacattatt tttttcggtt
     1081 tgtgcctgta tttgctttta ctatatctt
//
LOCUS       DAOSYE010000876         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_125974_length_1109_cov_2.701139, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000876 DAOSYE010000000
VERSION     DAOSYE010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125974_length_1109_cov_2.701139"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLU43_12290"
     CDS             <1..314
                     /locus_tag="PLU43_12290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO29236.1"
                     /translation="FYSLADVYVNLTLEDTFPTTNIEALACGTPVVTYKAGGSAESID
                     DTCGIAVERNSVQGVIAAIDKIIALRGRLYTQEHCRKRAEIYDKNIRFLEYVQEVYEG
                     M"
     gene            347..>1109
                     /locus_tag="PLU43_12295"
     CDS             347..>1109
                     /locus_tag="PLU43_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29237.1"
                     /translation="MKETSSFKELCKKFERSVIFFFEFVMYAACFATFFLLFAIDNRE
                     IIGLSRTAAVTMSTYVIMLFLLTGIYGKYDVGMRKTKQIVFSLVLAAVFTDIITYFEL
                     TIMKTNEANNATFKIENVGIFFSVMIIQTLLIILVTYAGNAFYFWINKAERCLIITSE
                     EEDSIRVAHALEEFKKRYHVCGIVRYDTEDLTERIVDADTVVLYEVPVELRTDIVDFC
                     YQNLKNIYFNPHIADILEQNSKPVVVDDISFFTSRF"
BASE COUNT          303 a          188 c          285 g          333 t
ORIGIN      
        1 ctttttattc gcttgcagat gtttatgtga atctgacgct ggaagatacc tttccaacga
       61 cgaatatcga ggcacttgcc tgcggaacgc ctgttgtgac gtataaggca ggcggcagtg
      121 cggagagcat cgacgataca tgcggcattg cagtggagag aaacagcgtg cagggtgtga
      181 ttgctgcaat agataagatc attgcgctgc ggggccggct gtatacgcag gagcattgca
      241 gaaagagagc ggaaatatat gataaaaaca ttcgttttct ggaatatgta caggaagtct
      301 acgaagggat gtaggcttta taagagtatc tgatcgggag ataagaatga aggaaacgag
      361 cagttttaaa gaattgtgta aaaaattcga aaggtctgtc attttctttt ttgaatttgt
      421 tatgtacgcc gcctgttttg cgacgttttt tctgctgttt gcaattgata acagggaaat
      481 tatagggttg tcaagaactg cggctgttac aatgtccacg tatgtcatta tgctgtttct
      541 tttaacaggt atttatggaa agtatgatgt cgggatgaga aagaccaagc agatcgtttt
      601 ttcccttgtg cttgccgcag tttttaccga tattatcaca tattttgaat tgacgatcat
      661 gaagacgaac gaagcgaaca acgcgacgtt caagatcgaa aatgtcggta ttttcttttc
      721 ggtcatgatc atacagacac tgctgattat tttggtgaca tatgccggca atgcatttta
      781 tttttggatc aataaagcgg aacgctgtct gatcattact tctgaggaag aggattccat
      841 aagagtggcg catgcactgg aggaatttaa aaagaggtat catgtctgcg ggatcgtacg
      901 gtatgataca gaggatctga cggaacggat cgtggatgcg gatacggtcg tgctgtatga
      961 ggtgccggtc gagctgcgta ccgatattgt tgatttctgt taccagaacc tgaaaaatat
     1021 ttatttcaat ccgcatattg cggatattct cgaacaaaat tccaagccgg tcgttgttga
     1081 cgatatttcg ttttttactt cgagatttc
//
LOCUS       DAOSYE010000877         1108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_126205_length_1108_cov_2.326686, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000877 DAOSYE010000000
VERSION     DAOSYE010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126205_length_1108_cov_2.326686"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..971)
                     /locus_tag="PLU43_12300"
     CDS             complement(120..971)
                     /locus_tag="PLU43_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOO29238.1"
                     /translation="MKKAALVLEGGANRGVFSSGAVDYLMKKNVYFPYVVGVSCGACN
                     TVDYVSRQPERTKDCMIIKEKAYKTVTVKGMIKNKSLYDMDMVFDKFPNRYIPFDYDT
                     YFASAVKSELVVTNCLNGKAEYLNEKSDRKRLMDICRASSSLPLAAPIVFIDGIPYMD
                     GGMADSIPIVHSLHLGYKKNVIISTKEKGYRKKHVKRQELLVSTVYREYPMLVKTVLR
                     RPEMYNRTMELIEDLERGGHVFVLRPNGLHISHAERNMEKMQAFYELGYDTMEEEFDK
                     MMEYLER"
BASE COUNT          263 a          285 c          178 g          382 t
ORIGIN      
        1 tccttccgaa ttcagatcct ccacttttct tttctcaatc agctcatatg tttttaactc
       61 ttcgattttc atgtaattct cccgtctttt tattctgtac gtcttatatt tgtatgctct
      121 taccgttcga gatactccat cattttatca aattcttctt ccattgtatc atatccgagc
      181 tcataaaatg cctgcatttt ttccatattc cgttccgcat gagaaatgtg cagaccattc
      241 ggccttaaaa caaacacatg acccccgcgc tccaggtctt cgatcagttc catggtacgg
      301 ttatacattt ccggccgtct gagcactgtt tttacaagca tcggatactc tctgtaaaca
      361 gtactgacca gcagttcctg tcttttcaca tgctttttac gatacccttt ttcctttgtg
      421 gaaataatta catttttctt atatcccaaa tgcagggaat gcacgatcgg gatcgaatcg
      481 gccattccgc cgtccatata tgggatcccg tcaataaaaa caattggtgc ggccagcgga
      541 agactgcttg atgccctgca aatatccatt aaacgctttc tgtcactttt ttcattcaga
      601 tattcggcct ttccgttcaa acagtttgtt acaacgagtt cactttttac agcacttgca
      661 aaatatgtgt cataatcaaa cggaatgtac cggtttggga acttatcaaa taccatatcc
      721 atgtcatata agcttttatt ttttatcatt ccctttacgg tgaccgtctt gtaggctttt
      781 tcttttatga tcatacagtc ctttgtccgt tccggctgtc tggatacata gtctaccgta
      841 ttacaggccc cgcaggaaac cccgactaca tagggaaaat atacgttttt tttcatcaga
      901 taatcgaccg cgcccgatga aaatacacca cgattcgctc cgccttccaa aaccaatgct
      961 gctttcttca tactgattcc ttctattctg tttttttatc tgcttcctga tgctcatgcc
     1021 tgatataggt gaccgcattt cctgagaggc gcatactttc cgcaagctcc tcaatatcgg
     1081 cgtccagttc cttagccagt tcttcaag
//
LOCUS       DAOSYE010000878         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_126789_length_1105_cov_2.647619, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000878 DAOSYE010000000
VERSION     DAOSYE010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126789_length_1105_cov_2.647619"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1068)
                     /locus_tag="PLU43_12305"
     CDS             complement(97..1068)
                     /locus_tag="PLU43_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29239.1"
                     /translation="MAEQIYSVKHLKKYYQVNKSIRLSEIKYVHAVDDMSFDIYKGQI
                     FGVVGESGSGKSTVGRCILKLIDSTDGQIVYKGEDITGYTRKQMKSFRRDMQMVFQNP
                     FSSFNPRLSIGSSLKEIGHVYKMGVKETDDRIKELLDLIKLPSHVLDRGAGELSGGQL
                     QRLAVARALMLNPTFIMADEPVSALDVSVQSQILNLLLDLRDQLDLTIMFISHELTVV
                     HHVCDAVAVMYLGALVELAPADELFRNTLHPYSQALISAKPKDHPREQKERIVLEGDI
                     PNAIDIPKGCRFATRCPNFIHGECNMVAPMLEEVAPGHFVACHMINH"
BASE COUNT          297 a          279 c          202 g          327 t
ORIGIN      
        1 atactttcag caatgctagc aaaaccaagg tctacgaatt atgaaatttc ttcaaataag
       61 ttaatatatt gaatacagca acacactgtt tccaactcaa tgattaatca tgtgacaggc
      121 aacaaaatgt cccggtgcaa cttcttccag catcggagcg accatattgc attccccatg
      181 gataaaattc ggacatctcg tggcaaaacg gcatcctttt ggtatatcga tcgcatttgg
      241 aatatcccct tccagcacga tcctttcttt ctgttcccgc ggatgatcct tcggttttgc
      301 cgatatcagc gcttgtgaat acggatgcag ggtattcctg aacagctcgt ctgcaggcgc
      361 caattctacg agcgctccaa gatacatcac agctaccgcg tcgcacacat gatggacaac
      421 cgttaattca tgtgaaataa acataattgt caggtccagc tgatctctta agtccagcag
      481 caaattaagg atctgggact gtaccgatac gtccagtgcg gatacaggct catccgccat
      541 aataaatgtc gggttcagca tcagggccct ggctaccgcc agccgctgca gctgaccgcc
      601 gctcaattca cccgcgcctc tgtccaatac atgactcggt aatttaatca aatcaagcag
      661 ttccttgatc ctgtcgtctg tttcttttac tcccatttta taaacatggc cgatctcttt
      721 taaagaactg ccgatcgaca gccttggatt aaatgatgaa aatggatttt gaaataccat
      781 ctgcatatcc ctgcgaaagg atttcatctg tttgcgggta taacctgtaa tgtcttcacc
      841 tttatatacg atctggccgt ctgttgagtc gatcagtttc aaaatacacc ttccgactgt
      901 tgatttgcct gacccgcttt cacctaccac accaaagatc tggcccttat aaatgtcaaa
      961 gctcatatca tcaacagcat gaacatattt gatttcactt aagcgaatcg acttgttgac
     1021 ctgataatat ttcttcaagt gttttaccga ataaatttgt tccgccatgc tcttttcctc
     1081 cgcatattat tttgcattgt agatt
//
LOCUS       DAOSYE010000879         1102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_127399_length_1102_cov_2.638968, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000879 DAOSYE010000000
VERSION     DAOSYE010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127399_length_1102_cov_2.638968"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..799
                     /locus_tag="PLU43_12310"
     CDS             191..799
                     /locus_tag="PLU43_12310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO29240.1"
                     /translation="MFRRLFQNTRKPKGFWGRMMLRSMNGGHTPLSLWGLSLIHPAPN
                     ARILDAGCGGGANISRLMQLCPKGFVDGIDYSEESVAYSKKKNAASLGTHCDIKYGNI
                     EKLPYADQTFDLVTAFETVYFWPDPEKAFRQIIRVLKPGGQFLLVCEMDDANNTTWTN
                     LIDGMTIYAGEDLKNRLLHAGFTHVGLKRNHTSWIALLAEKE"
     gene            complement(818..>1102)
                     /locus_tag="PLU43_12315"
     CDS             complement(818..>1102)
                     /locus_tag="PLU43_12315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO29241.1"
                     /translation="RNSVREYDLVCRYGGDEFLILAEADEEDVAARISGMIGREFDVF
                     NYTEIKKYHLSFSIGYAYWEPAEGRSLSELICEADAKMYQDKGIYRAEEG"
BASE COUNT          313 a          266 c          226 g          297 t
ORIGIN      
        1 aatgctaatc aatgaatagt cccttccaca cttccatatt gacaacttat taaaagaatg
       61 ataatttatg gttagttgtt tctaacattt ttgaaacatt tatacagctg ttataaaagg
      121 aggactccca cacaacctta acaacagtaa aagccatggc ctgtttctgc tgccggaaag
      181 gagacacata atgtttcgac gactttttca aaatacaaga aaaccaaagg gcttttgggg
      241 ccgtatgatg ctccgcagta tgaacggcgg ccatacgccg ctttccctct ggggcctttc
      301 tctgatccat cctgctccca atgcaaggat actggatgcc ggatgcggcg gaggcgccaa
      361 tatttcccgg ctgatgcagc tttgcccaaa aggatttgtg gatggaatcg attactccga
      421 ggaaagcgtg gcatacagta aaaagaaaaa tgccgcatct cttggaacac attgcgacat
      481 aaagtacggt aatatagaaa aactgcctta cgccgatcag acgtttgatc tggtcacagc
      541 gtttgaaaca gtttacttct ggccggatcc tgagaaagca ttccgacaga tcatacgagt
      601 tttaaaacca ggcggacaat tcctgctggt gtgcgaaatg gatgacgcaa ataatactac
      661 atggacaaat ctgattgacg gtatgacgat ctatgccgga gaagacttaa aaaaccggct
      721 tttacatgcg ggctttacac atgttggtct gaaaagaaat catacaagct ggatcgccct
      781 gctcgcagaa aaagaataaa gcttcgtacg ctgttcatca gccttcctct gccctgtaaa
      841 ttcctttatc ctgatacatc tttgcatcgg cttcacaaat cagttcactt aaggatcttc
      901 cctctgccgg ttcccagtac gcatatccga tgctgaagct taagtgatat tttttgattt
      961 ccgtataatt gaatacgtca aattctcggc cgatcattcc gctgatccgt gctgccacat
     1021 cctcttcatc agcctcggcc agaatcagaa attcatcgcc gccgtacctg cagactaaat
     1081 catattcccg cacgctgttt ct
//
LOCUS       DAOSYE010000880         1101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_127562_length_1101_cov_3.208413, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000880 DAOSYE010000000
VERSION     DAOSYE010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127562_length_1101_cov_3.208413"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLU43_12320"
     CDS             <1..929
                     /locus_tag="PLU43_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO29242.1"
                     /translation="MTGYEVCRMLKAKEETKEIPVIFLTAMTDIDSKTTGFEAGAVDY
                     ITKPFDIMEVKERVKTHLLLRFTQYELEAQKQTLEEKVRERTQELLLTQEATIEALAC
                     LAEYRDPETGGHIKRTKNYIKVMAERLKEMPEYKEELTDEVIGLLYMSAPLHDIGKIG
                     IRDEILMKPGKLTEEEFEEMKTHCNIGYSALKSASKKLGENSFLKYAMEFSKFHQEKW
                     DGSGYPDGIAGKEIPLSGRMMALADVYDALISKRVYKPPYSHEKAVRIIQEEKGLHFD
                     PELVDIFMELQEKFREIAIEYADFTEEKLQLT"
BASE COUNT          380 a          179 c          283 g          259 t
ORIGIN      
        1 gaatgacagg gtatgaagta tgcagaatgc tgaaggcaaa ggaagaaaca aaagaaatac
       61 ctgtgatctt tctgacggca atgacggata ttgacagtaa aacaacaggg tttgaagcag
      121 gggctgttga ttatattaca aaaccgtttg acatcatgga agtaaaggaa cgggtcaaaa
      181 cacatctttt actgcggttt actcagtacg aactggaagc acagaaacag acacttgaag
      241 aaaaagtccg tgaacgcacg caggaactgc tgctgacgca ggaggcaacg atcgaggcgc
      301 tggcctgtct ggcagagtac cgggatccgg aaaccggagg acatatcaag cgtacgaaaa
      361 attatataaa agtaatggct gagagactga aggaaatgcc ggagtataag gaggagctta
      421 cggatgaagt gatcggactt ctgtatatgt cggcgccgct gcatgatatc gggaagatcg
      481 gaatacggga tgaaatattg atgaaaccgg ggaaactgac agaggaagaa tttgaagaaa
      541 tgaaaacaca ttgcaatatc ggctactcgg ctttaaaatc cgcttccaag aagctgggag
      601 aaaattcatt tttaaaatat gcaatggaat tttctaaatt tcatcaggaa aaatgggatg
      661 gctccggtta tccggatgga atagcaggaa aagagatccc attatccggg agaatgatgg
      721 cgcttgcgga cgtatatgat gccctgatca gcaagcgggt gtataaaccg ccgtattcac
      781 atgaaaaagc tgtccggatc attcaggagg aaaaaggtct tcattttgat ccggaactag
      841 tggatatttt tatggagctg caggagaaat tcagggagat cgcaattgaa tatgcggatt
      901 ttacggaaga aaagctgcag ctcacatgac atttatacaa agatcctgga cggacaggat
      961 acagtttgcc ttgacaatta cagaaacaca agtatataat attaacattg ttaattttat
     1021 atacttgtgt ttctgtttat aggaggaaaa atgagcggaa atcagaatct gaaaattgaa
     1081 agaatgaagt cttattttac g
//
LOCUS       DAOSYE010000881         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_128052_length_1099_cov_2.275862, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000881 DAOSYE010000000
VERSION     DAOSYE010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128052_length_1099_cov_2.275862"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..903)
                     /locus_tag="PLU43_12325"
     CDS             complement(118..903)
                     /locus_tag="PLU43_12325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO29243.1"
                     /translation="MSFFSDRMKVCFPDITYDFYRTYVKTNRIDFEQYPLDDDFQIRV
                     EDYINTDRDIILANPNNPTGQVLPLEEIEKIVASRPNRMVILDEAYVDYENESAVPLT
                     KKYANLIVVQTFSKSRNLAGARIGFAIASKEIIEDMNKIKFTFNPFNMGELAIAAGTA
                     AVGDDVYLKQCMHQILKARAYTKTELERLGFYVMESHTNFLLVKHPEISAAILCRALK
                     NRGILVRHYNEPRLVSYLRITIGLEKEMSQLIREIHAMMQEGI"
     gene            complement(900..>1099)
                     /locus_tag="PLU43_12330"
     CDS             complement(900..>1099)
                     /locus_tag="PLU43_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29244.1"
                     /translation="AWLLINEIANVLRASKDEIYLSMLKSYGQSSVVSVVEEAAEMFM
                     ASDMIRYLSETERMKYWALRL"
BASE COUNT          274 a          295 c          190 g          340 t
ORIGIN      
        1 cctccgtata gatccagctt cccgcagcgt ccgtcacacc gatcgtcgtc gcccgcttcg
       61 caacaggcgg caatactttt aagcttctct catataatgt catctcgcac ctctttttta
      121 tattccttcc tgcatcattg catggatttc cctgatcagc tgactcattt ccttttccag
      181 gccaatggta attctcaaat aggagaccag ccttggctca ttataatgcc ggactaagat
      241 ccctctgttt ttcaatgccc ggcacaggat cgcagcggat atttcaggat gtttcactaa
      301 aagaaaattc gtatgcgact ccataacata aaagccgagc ctttccagct ctgtttttgt
      361 atacgctctt gcttttagta tctgatgcat acactgcttt aaatacacat catctccgac
      421 tgcagccgtt cccgctgcga tcgccagttc tcccatatta aacggattga acgtgaactt
      481 gatcttattc atatcttcaa tgatctcttt tgaggcaatg gcaaatccga ttctggctcc
      541 tgccagattt ctcgatttgg aaaacgtctg cacaacgatc aggttcgcat atttttttgt
      601 cagcggcact gcggattcat tctcataatc gacataagcc tcgtccagaa tcaccattct
      661 gtttggtctg cttgcaacta ttttctctat ttcttccaga gggagcacct gtcctgtcgg
      721 attattggga tttgccagaa taatatccct gtccgtattg atataatctt cgactctgat
      781 ctgaaaatca tcatccagcg gatactgctc aaaatcgatc ctgtttgtct ttacataagt
      841 cctgtaaaaa tcatacgtaa tatccggaaa gcagaccttc atcctgtcgg aaaaaaaact
      901 cataaacgca aagcccagta cttcatccgc tccgtttccg acaaatacct gatcatatcc
      961 gacgccataa acatttccgc agcttcttct accacgctca ccacgcttga ttgaccgtag
     1021 cttttgagca ttgataggta gatttcatcc ttgcttgccc ttagaacatt ggcaatctcg
     1081 ttgattaaca gccaagcat
//
LOCUS       DAOSYE010000882         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_128781_length_1095_cov_3.110577, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000882 DAOSYE010000000
VERSION     DAOSYE010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128781_length_1095_cov_3.110577"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLU43_12335"
     CDS             <1..830
                     /locus_tag="PLU43_12335"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO29245.1"
                     /translation="GALNGLTRVRGFTQDDAHIICAPEQMLDEVISALKFSLYILRSF
                     GLHDYKVYVATKPKSKSIGSKEDWEHAISVLKDALQQVGLPFDIDEGGGAFYGPKIDV
                     KLKDALNREWQCSTVQFDFNLPQRFDMYYIGPDGQKHVPCMIHRALFGSVERFIALLI
                     EHYQGDFPLWLAPVQFAIVPIGEKHNDYCKKLEKTFKKQLLRVTANYEDLHMREKIKR
                     FEMEKIPFILIVGDKDIAADGFSVRSRQSGNLGFMTFEQLCGHIEPELEKGRPQYLL"
     gene            complement(825..>1095)
                     /locus_tag="PLU43_12340"
     CDS             complement(825..>1095)
                     /locus_tag="PLU43_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29246.1"
                     /translation="EFLYFYRLDQRDKASGLQDEEFELSKLFEPFLFFITKSCNKTQE
                     EAVLLIKSMIYMVHGLLTLHLSGNDELTSAAMEMDLKEIISMLLS"
BASE COUNT          304 a          254 c          262 g          275 t
ORIGIN      
        1 ccggtgccct gaacgggctt acaagggtcc gcggctttac gcaggatgac gcccatatca
       61 tctgtgcacc tgagcagatg cttgacgaag taatcagtgc actgaaattc tctctttata
      121 ttctgcgttc gttcgggctg cacgattaca aagtatatgt tgcaacaaag ccgaaatcca
      181 aatccatcgg ttcaaaagaa gactgggaac atgcgatcag cgttttaaaa gatgccctgc
      241 agcaggtcgg gcttccgttt gatatcgacg aggggggcgg tgccttttac ggacctaaga
      301 tcgatgtgaa actgaaagat gcgctgaacc gggaatggca gtgcagcacg gttcaatttg
      361 attttaatct gccgcaaaga tttgatatgt attacattgg cccggacggg cagaaacatg
      421 tgccctgcat gatccaccgt gccctgttcg gcagtgtcga acggttcatt gcactgctca
      481 tagaacacta tcagggtgat ttcccgctat ggctggctcc cgtccagttc gccattgttc
      541 ctatcggcga aaagcataac gattactgca aaaaacttga gaaaactttc aaaaaacagc
      601 tgcttcgggt aacggcaaat tatgaagacc tgcatatgag agaaaagatc aaacgctttg
      661 aaatggaaaa gatcccgttc atcctgatcg tcggagacaa agatatcgct gcagacggct
      721 tttcggtaag aagcaggcag tccggcaatt taggctttat gacttttgaa cagctgtgcg
      781 gtcatattga accggaactg gaaaaaggcc gtccgcagta tcttctatga cagcagcatg
      841 gaaatgatct ctttcagatc catttccatg gccgccgatg tcagctcatc atttccggac
      901 aggtgcaggg tcagaagacc atgcaccata tagatcatgg atttgatcag caaaactgct
      961 tcttcctgtg tcttattgca ggactttgtg ataaagaaca gaaacggttc aaataatttt
     1021 gaaagttcaa attcctcatc ctgcagcccg gaagccttgt cccgctgatc cagtctgtaa
     1081 aagtacaaaa actca
//
LOCUS       DAOSYE010000883         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_128822_length_1095_cov_2.525000, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000883 DAOSYE010000000
VERSION     DAOSYE010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128822_length_1095_cov_2.525000"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLU43_12345"
     CDS             <1..436
                     /locus_tag="PLU43_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO29247.1"
                     /translation="LLSLYEINPEWLEIEITESVFINDFDEVIEKINTLRKLGVKISL
                     DDFGTGFSSLSYLKVMPIDTLKIDKSFIDTAINDPSTNIITESVVTMARRLGLETIAE
                     GVETDAQYEYLKKIQCDSVQGYLFGRPMSRTDFEKLIVRQLP"
     gene            456..>1095
                     /locus_tag="PLU43_12350"
     CDS             456..>1095
                     /locus_tag="PLU43_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29248.1"
                     /translation="MISGRTMEKQYLENFYARSENEIVVLYGQENVGKTSLIRQFCKE
                     KDFFYYLARPCSEKEQIYLFGCELKEALPPGSDESDSCSGIWSRMLSVKAEKRVIVID
                     EFQNIVKNSPAFMQEIVRTARDKWSNQPVLFILCSSSVYWVENLMVKKLGEAAYEISG
                     LLKINELKFLDIVRKFKKYKLPGCVETYAILGGIPGFWEFWDDSVTVRENVCG"
BASE COUNT          328 a          175 c          274 g          318 t
ORIGIN      
        1 tctgctgtcg ctttatgaga tcaatccgga atggctggag atcgaaatta ctgagagcgt
       61 atttatcaat gattttgacg aagtcataga aaagatcaat acattgcgga aacttggcgt
      121 taaaatatcc ttagatgatt ttggcacagg gttttcgtct ctttcttatt taaaggtaat
      181 gccgatcgat acgttgaaaa tcgacaaatc ttttattgat accgcaatta atgatccctc
      241 tacgaatatt atcacggagt ctgttgtgac tatggcaaga cggcttggac ttgagacgat
      301 tgccgaaggt gtggagacgg atgcacagta tgaatatctg aaaaaaattc agtgcgatag
      361 tgtacaggga tatctgtttg gcaggccaat gtccagaact gattttgaaa agctgatcgt
      421 acggcagctt ccatgatcat ttaaggagag aatttatgat ttctggaaga actatggaaa
      481 aacaatatct cgagaacttt tatgcgcgca gtgagaatga aatcgttgtt ctgtacggac
      541 aggaaaatgt cgggaaaaca tcactgatca ggcagttctg taaggagaag gatttttttt
      601 attatcttgc aagaccatgt tctgaaaaag aacagatcta tctgtttggc tgcgaattaa
      661 aagaagcgct gccgccaggg tcagacgaat cggatagttg ttccgggatt tggagcagga
      721 tgctttccgt gaaagcggaa aaacgggtga tcgtaataga tgaattccag aatatcgtaa
      781 aaaacagtcc ggcttttatg caggaaattg taaggaccgc acgtgataag tggagtaatc
      841 agccggtgtt gtttatttta tgttcttctt ctgtctactg ggttgaaaat ctcatggtta
      901 aaaaattggg agaggcagcc tatgaaattt ccggtctctt gaaaataaat gagctgaaat
      961 ttttggatat tgtcagaaaa tttaaaaaat ataagctgcc gggctgtgtg gagacatatg
     1021 cgatcctcgg cggtattccg ggtttttggg aattttggga cgactccgta acagtcaggg
     1081 aaaacgtgtg cggac
//
LOCUS       DAOSYE010000884         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_128955_length_1094_cov_3.640038, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000884 DAOSYE010000000
VERSION     DAOSYE010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128955_length_1094_cov_3.640038"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..736
                     /locus_tag="PLU43_12355"
     CDS             98..736
                     /locus_tag="PLU43_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29249.1"
                     /translation="MRLPIFTTITVLSALTWLYMRRSNRKVGQDTNEFLAREQFANSI
                     RKQPLDSLSYITVPLDSFPFIEDTENEILQECQKTIRSLSEQKIVNLTGISNTDLKLQ
                     YGVANITVLSEYDQNFTVLARTLCAWGKELYGLGLKKEAAVVLEFGISCKTDIKSNYM
                     LLADLYIEDHEPDKIDALTETAKTLNSLMKNSILASLAEKRNASGEAPSEES"
     gene            complement(733..>1094)
                     /locus_tag="PLU43_12360"
     CDS             complement(733..>1094)
                     /locus_tag="PLU43_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO29250.1"
                     /translation="RTLHTEYHGEVPRTLEELTALAGVGRKTANVIRGNIFHEPSIVV
                     DTHVKRISKKLGFTKEDDPEKVEYDLMKVLPEDHWILYNIQIITFGRDICMARNPQCE
                     RCRLTKYCSFYKNERKN"
BASE COUNT          310 a          262 c          213 g          309 t
ORIGIN      
        1 cagtaccagt ataatgataa taatactgga aatgatcctt tttgttttgt tgttattaaa
       61 attcatgatt ctcacctctc ttgaaaggtt attatatatg agattgccga tttttacaac
      121 cattaccgtt ctttccgcac tgacatggct ttatatgaga agatccaaca gaaaggtcgg
      181 acaggataca aatgaatttc ttgcccggga acagtttgcc aacagcatca ggaaacagcc
      241 tttggattct ctttcatata ttactgttcc gctggattcc ttccccttta tcgaggacac
      301 ggaaaatgaa atcctacagg aatgccagaa aacgatccgc tcgctttccg aacagaagat
      361 cgtcaacctg accggtattt ccaacactga tctgaagctg caatatggtg ttgcaaacat
      421 tactgttctg tcggaatatg accagaattt cacagtcctc gcaagaacgc tttgtgcctg
      481 gggaaaagag ctgtacggac tcggcctgaa aaaagaagcc gccgttgtgc tggaattcgg
      541 catttcctgc aaaaccgaca tcaaaagcaa ttatatgctg cttgccgatc tttatattga
      601 agaccatgag cctgacaaaa tagacgcatt aacagaaact gcgaaaaccc tgaacagcct
      661 catgaaaaat tccattctcg catctcttgc ggaaaaaaga aatgcttccg gcgaagctcc
      721 gtcagaagag agctaatttt ttcgctcatt tttataaaaa ctgcagtatt ttgtcagacg
      781 gcagcgttcg cactgcggat ttcttgccat acagatatcc ctgccaaacg taatgatctg
      841 gatattatat aagatccagt gatcctctgg cagcactttc atcagatcat attcgacttt
      901 ttccggatca tcctcttttg taaacccaag cttcttggaa attctcttga catgcgtatc
      961 cacgacaata cttggctcat ggaagatatt cccgcgtatt acatttgccg tctttctgcc
     1021 gacacccgca agagcggtca gttcctcaag cgtccgcggc acctcaccat ggtattccgt
     1081 atgaagcgtc cgac
//
LOCUS       DAOSYE010000885         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_129024_length_1094_cov_2.438884, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000885 DAOSYE010000000
VERSION     DAOSYE010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129024_length_1094_cov_2.438884"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>1094)
                     /gene="ilvC"
                     /locus_tag="PLU43_12365"
     CDS             complement(81..>1094)
                     /gene="ilvC"
                     /locus_tag="PLU43_12365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOO29251.1"
                     /translation="KIFYQEDCNLSMLDGKTIAVIGYGSQGHAHALNARESGCNVIIG
                     LYEGSKSWDKAVKQGFEVYTAAEAAKKADIIMILINDEKQAAMYKKDIAPNLEAGNML
                     MFAHGFSIHFGQIIPPKDVDVTMIAPKGPGHTVRSEYQAGKGVPCLVAVQQDASGKAL
                     DKALAYALAIGGARAGVLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAGYD
                     ERNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYVTGPKLITEETKKTMKKI
                     LSDIQDGSFAKEFLLDMSSAGGQAHFRAMRNLAAEHPSEKVGEEIRKLYSWNNENDKL
                     INN"
BASE COUNT          273 a          283 c          194 g          344 t
ORIGIN      
        1 tgtcaataaa tttgatatca attcaaattt aaaaaacacg gacagctcca tatgagccat
       61 ccgtgttgac tttttcgatc ttagttgttg atcagtttat cgttctcatt gttccagctg
      121 taaagcttac ggatctcttc accgactttt tccgaaggat gctctgcagc aaggtttctc
      181 attgcccgga aatgtgcctg tccacctgct gaggacatat ccagaaggaa ttcctttgca
      241 aaggaaccat cctgtatgtc acttaatatc ttcttcatgg tcttctttgt ttcttccgta
      301 ataagcttcg gtcctgtaac ataatcgccg tattcagccg tattggaaat agaatatctc
      361 attcctgcaa atcctgactg ataaatcaga tcaacgatca gcttcatttc atggatacac
      421 tcaaaatatg catttctttc atcatagcct gcttcaacaa gcgtttcaaa gcctgcctgc
      481 atcagtgcac atacaccgcc gcaaagcaca gcctgctcgc cgaacagatc tgtttctgtc
      541 tctgttctga aagttgtctc caacacgcct gctcttgcgc cgccgattgc aagtgcataa
      601 gccagagcct tatcaagagc ctttccggaa gcgtcctgct gtaccgctac aagacaggga
      661 actcctttgc ctgcctgata ttcgcttctt accgtatgac cgggtccttt tggtgcgatc
      721 attgttacgt cgacatcctt cggcggtatg atctgtccga aatgaattga aaatccatgc
      781 gcaaacatca gcatatttcc tgcttccaaa tttggcgcga tatccttttt atacattgca
      841 gcctgttttt catcattgat cagaatcatg ataatatcgg ctttctttgc agcttcagct
      901 gctgtatata cttcaaatcc ctgctttact gccttgtccc atgacttaga tccttcatac
      961 agaccaataa tgacattgca gcccgactcc cttgcattca atgcatgtgc atgtccctga
     1021 ctgccatatc cgatgacagc aattgtcttt ccgtcaagca tagacagatt acaatcttcc
     1081 tggtaaaaaa tttt
//
LOCUS       DAOSYE010000886         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_129648_length_1091_cov_2.667954, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000886 DAOSYE010000000
VERSION     DAOSYE010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129648_length_1091_cov_2.667954"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLU43_12370"
     CDS             <1..531
                     /locus_tag="PLU43_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOO29252.1"
                     /translation="ARFILPLFLTEDPEKIILRLCGEFRWELCRRIQGARWNDIGERS
                     LTSDYCDYLQIYKRSRDLSSEIKEKIKLSYSKYRNSSKEMFVHDYMDYMQYESAGLLR
                     LNKLSRAILFQYCPFSSAVRETVSLNQFYKDLVDRYKIKRAHSIRLCDLSMQKIEKAG
                     HQIPHPIREHRRFLAL"
     gene            complement(534..>1091)
                     /locus_tag="PLU43_12375"
     CDS             complement(534..>1091)
                     /locus_tag="PLU43_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29253.1"
                     /translation="PVGEQLTQTSLFSRLFDIRLHSVFPAMKAYGDAYDEYNVPLAMM
                     KSSLFGEYNLAKEWTAITPFAVVLFISGVLLAIAAFAAMLYMLFSKRSSLQAEWKLFL
                     GVLYVSLLAFYCKFAFGSSNFSAQDFRYIALIIVPEAIFLGSFVDTLDFGKRQQKLIG
                     CTVMGAAAVFAASSFAVYMMLGFAR"
BASE COUNT          337 a          274 c          233 g          247 t
ORIGIN      
        1 gcacgtttca ttctgccgct gtttctgaca gaagacccgg aaaagatcat actgcgcctg
       61 tgcggggaat ttcgctggga attatgccgc cgtattcagg gtgcacgctg gaacgacatc
      121 ggcgaacggt cactgacaag tgattactgc gactacttac agatctataa aagaagccgt
      181 gacctttctt cggaaataaa agaaaaaatc aagctgtcct attcaaaata caggaacagc
      241 tcaaaagaaa tgtttgtaca cgattatatg gattatatgc aatatgaaag tgcaggcctg
      301 ctgcgtctga acaaactctc acgggcgatc ctgtttcagt actgcccatt ttcctctgca
      361 gtccgcgaga ctgtcagtct gaaccagttc tataaagatc tggtcgaccg atataagatc
      421 aaacgggcgc acagcatccg tctttgcgac ctgtccatgc agaaaataga aaaagcgggg
      481 catcagatcc cgcatccgat cagggaacac cggcgttttc tggcactgta agcttatctt
      541 gcaaagccaa gcatcatata tacagcaaaa gaacttgctg caaagactgc cgcggctccc
      601 atgaccgtac agccaatcag tttctgctgt ctttttccaa agtcaagcgt gtccacaaat
      661 gatccaagaa aaatcgcctc cggtacaatg atcagtgcaa tataccgaaa atcctgcgca
      721 ctgaaattag aactgccgaa agcaaatttg cagtaaaaag caagcaggga aacgtaaaga
      781 acccctaaaa acaatttcca ctccgcctgc aggctgctcc tttttgagaa aagcatatac
      841 agcattgctg caaatgccgc gattgcaagc agaactcccg aaataaaaag aacgaccgca
      901 aacggcgtga ttgccgtcca ttcctttgca agattatatt caccaaacaa agaggacttc
      961 atcatggcaa gcggcacatt atattcatca tacgcgtccc catacgcctt catggcagga
     1021 aatacgctgt gaagcctgat atcaaacagt ctggaaaaaa gagacgtctg tgtcagctgc
     1081 tctcctaccg g
//
LOCUS       DAOSYE010000887         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_129663_length_1091_cov_2.557915, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000887 DAOSYE010000000
VERSION     DAOSYE010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129663_length_1091_cov_2.557915"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLU43_12380"
     CDS             complement(<1..739)
                     /locus_tag="PLU43_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29254.1"
                     /translation="MVKYKALIAEDNLFNTDFLKAQLEALDVICDSAKNGLAALEMVK
                     KGSYDIVFMDCQMPVMNGITAAAKIRELGGRYLQLPIIALTADCSIEDEKTYLKEGMS
                     EVLRKPIELEDLGRILETCLHYMEPAETQPPKAEVLDLKENSLYFREAVEKLMRDMNF
                     SYEAAYGFICEYVDTITELLLKMEMAYEQSDYLKITNLAHQMSGISCNLRLKEFQAAA
                     KDIEIKSIHQELGVIEKIKHVKEMTALF"
     gene            complement(815..>1091)
                     /locus_tag="PLU43_12385"
     CDS             complement(815..>1091)
                     /locus_tag="PLU43_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29255.1"
                     /translation="AVYPTKLFEASLALIGLIPIIRIYLFRKKRIQKGVCFLLYAVWF
                     SAMRLAVLPLRSFPYEAVITHVFYPALYLTVIAAGSLLLWKKTRSVI"
BASE COUNT          325 a          250 c          181 g          335 t
ORIGIN      
        1 caaacagcgc tgtcatctct tttacatgct tgattttttc tattacgcca agctcctgat
       61 gaatgctttt gatctcaata tcctttgcgg ccgcctgaaa ttcctttaaa cgcaaattac
      121 aggaaatccc gctcatctga tgtgcaaggt tcgtgatttt taaataatca gactgctcat
      181 aagccatttc catttttaac aataattctg ttattgtatc aacatattca caaataaatc
      241 catatgctgc ctcgtatgaa aaattcatat cacgcattaa tttttctacg gcctcacgaa
      301 aatataaaga attttcctta agatccaata cctctgcttt tggcggctgc gtttctgcag
      361 gttccatgta atggagacag gtctccagta tcctgccaag atcctcaagc tctatcggct
      421 ttcgcagaac ttcactcatt ccttctttga gataggtctt ttcatcctct atggagcagt
      481 cagcagtcaa tgctataatg ggcagctgca aatagcgccc gccaagctct cgtattttgg
      541 cagcagctgt gattccgttc ataaccggca tctggcagtc cataaaaaca atatcatagc
      601 ttcctttttt gaccatttct aacgctgcaa gaccattttt tgcagaatca caaataacat
      661 ccagggcctc cagctgtgcc tttaaaaaat cagtattaaa taggttgtct tcagcaatca
      721 gtgctttata ttttaccata tttatcctca catctgcata taacatttac ctgttggaat
      781 tattatagca aacgtacggc acataatacc agtgtcaaat gacacttctg gtttttttcc
      841 ataataacaa tgatccagct gcaataacag ttaaatagag ggccggataa aatacatgtg
      901 ttataactgc ttcatacgga aaacttctga gcggaagaac agcaagacgc atggcactga
      961 accataccgc ataaagcaaa aaacaaacgc ctttttgaat cctttttttt cgaaaaagat
     1021 aaattcggat aatcggtatc agtccgatca atgccaacga tgcttcaaaa agcttggtcg
     1081 gatagaccgc g
//
LOCUS       DAOSYE010000888         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_129824_length_1090_cov_3.123671, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000888 DAOSYE010000000
VERSION     DAOSYE010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129824_length_1090_cov_3.123671"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLU43_12390"
     CDS             <1..617
                     /locus_tag="PLU43_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HOO29256.1"
                     /translation="LDMKHYPRQGQFDYFCNMAYPYVGMTVNLDITAFMAYIKKEKLP
                     FYLSMLYAAADTANAIPEFRQRIRGDQIIEFDHCTPSYILALDNGSYCYCSANERLPF
                     PKYLEDALYLQEEAKKMTTINEEENAEKLLFFSTIPWASYTAIVQPVPTPADSNPRIT
                     WGKYFKTEGRLKIPVTVLANHALMDGYHISKFFQELQQRFDNTD"
     gene            complement(618..>1090)
                     /locus_tag="PLU43_12395"
     CDS             complement(618..>1090)
                     /locus_tag="PLU43_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOO29257.1"
                     /translation="KVRAFFKKYDMEERIQEFTVSSATVELAAAALHCEPGRIAKTLS
                     FMVDGKAMLIVTAGDVKIDNPKYKAQFGTKAKMLTPENAEALIGHAVGGVCPFAVNDG
                     VPVCLDVSLKRFETVFPACGSSNSAIELTPEELEKYAAPVSWIDVCKGWAQNQS"
BASE COUNT          293 a          286 c          221 g          290 t
ORIGIN      
        1 gcttagacat gaaacactac ccgcggcagg ggcagtttga ttatttttgc aatatggctt
       61 atccctatgt tggaatgact gtaaatctgg acattacagc ttttatggcc tatatcaaaa
      121 aagaaaaact gccattttat ttgtcaatgc tgtacgctgc cgctgatacg gcaaatgcaa
      181 ttccggaatt tcgccagcgt atccggggtg atcaaatcat tgaattcgat cactgtacac
      241 cttcctatat tctcgccctt gacaatggaa gttactgcta ctgctctgca aacgagcgcc
      301 tgccgtttcc aaaatatctg gaagatgccc tgtatctgca ggaagaagcc aaaaaaatga
      361 ctaccataaa tgaagaagaa aacgctgaaa aactgctttt cttttctacg atcccatggg
      421 caagctatac cgcaattgtc cagccggtac cgacacccgc agacagcaac ccacgcatta
      481 cctggggaaa atattttaag acggaaggcc gtttaaaaat tcctgtaacc gttcttgcca
      541 accatgcact gatggacgga taccatatct caaaattctt tcaggagctt cagcagcgct
      601 ttgacaacac cgactgacta tgattgattc tgcgcccatc ctttgcagac atcgatccag
      661 gaaacaggag ccgcgtattt ctccagctct tccggtgtca gctctattgc actgttgctg
      721 cttccgcagg ccggaaacac ggtctcaaaa cgcttgagcg acacatccag acagacaggc
      781 acgccgtcat tgaccgcaaa aggacagaca ccgccaacgg catggccgat caaggcttcc
      841 gcattctcag gtgtcagcat ttttgctttt gttccaaatt gagctttata ctttggattg
      901 tcgatcttta catctcctgc cgtaacgatc agcattgctt ttccatccac cataaaagaa
      961 agcgtctttg cgatcctgcc cggctcacaa tgcagcgcgg ctgccgcaag ttcaacggtt
     1021 gcgcttgata cggtaaactc ctgtatacgc tcttccatat cgtacttttt aaaaaaagct
     1081 cttacttttt
//
LOCUS       DAOSYE010000889         1086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_130648_length_1086_cov_3.171678, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000889 DAOSYE010000000
VERSION     DAOSYE010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130648_length_1086_cov_3.171678"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLU43_12400"
     CDS             <1..582
                     /locus_tag="PLU43_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29258.1"
                     /translation="TLAMTYILKGIVQLYTNGQSIGGVDKIRWLGQGTIAGIPVPVII
                     MVLMVILIAILMKRTKFGTYIYAIGGSEKAAIASGINTNRNKRIIFALSGALTGLAGV
                     VLMARLMAGMPSVGDGYEFDAITAVIVGGTSFLGGIGTVTGAFIGSIIVGLINNILNL
                     LHVSTQYQLIVKGLMIAAAVIVDIKTKEDKKSR"
     gene            658..>1086
                     /locus_tag="PLU43_12405"
     CDS             658..>1086
                     /locus_tag="PLU43_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29259.1"
                     /translation="MKKKLLSMLLTAAVVSAMLAGCGTTASEESSEAVDADQETAQTE
                     EAADTEEESETASTDADFLVGFANNSDTYNYCAKFRTYLKEATEAKGISIMVTDAGGD
                     TNVQNGQIDDFIVQNANIVSAISNDLDGSVPALEAAKTAGI"
BASE COUNT          336 a          190 c          292 g          268 t
ORIGIN      
        1 acattggcaa tgacctatat tctcaaagga attgtgcagt tatatacgaa tggacagagc
       61 atcggcggtg tcgataagat cagatggctt ggacagggta cgatagcagg cattccggta
      121 cctgtcatca ttatggtatt aatggtaata ttgattgcca ttctaatgaa aagaacgaaa
      181 ttcggaacgt atatttatgc gatcggcggc agtgagaaag cagcgattgc atccgggatc
      241 aatacaaatc ggaataaacg aatcattttt gcactgagcg gcgcactgac cggacttgcg
      301 ggcgttgtgc tgatggcaag gctgatggca ggcatgccgt ccgtaggcga cggatatgag
      361 tttgatgcga ttacggcggt tattgtcgga ggtacaagct tccttggcgg gatcggtacg
      421 gttaccggtg cattcatcgg ctcgatcatt gtcggactga tcaataatat attgaattta
      481 ctgcatgtgt ccacacagta tcaattgatc gtaaaaggtc tgatgatcgc agctgccgtg
      541 attgtagata ttaaaacaaa ggaagataaa aagagcagat aaataatatt gatgcatgta
      601 cacgggacat gccttgatat aaatatttta tgtgtttaca cacagaaggg agaaaaaatg
      661 aaaaagaagt tattaagtat gttattaacg gctgcggtcg tttccgcaat gctggcaggc
      721 tgcggaacaa ctgcttctga ggagagcagc gaagcagttg atgcggatca ggaaacagcg
      781 cagacagagg aggcggcaga taccgaagaa gaaagtgaaa cagcatcgac agatgcggat
      841 tttcttgttg gatttgcaaa taatagtgat acgtacaact attgtgcaaa attcaggacc
      901 tatttaaaag aggcgaccga agcaaaagga atttccatta tggtaacgga tgcaggcggc
      961 gatacgaatg tacagaatgg gcagatcgat gattttatcg tacagaatgc aaatatcgtt
     1021 tcagcgatca gcaatgatct ggacggttcc gtaccggcac ttgaagcagc aaaaactgcg
     1081 gggatt
//
LOCUS       DAOSYE010000890         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_131887_length_1080_cov_2.930732, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000890 DAOSYE010000000
VERSION     DAOSYE010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131887_length_1080_cov_2.930732"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..>1080)
                     /locus_tag="PLU43_12410"
     CDS             complement(241..>1080)
                     /locus_tag="PLU43_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29260.1"
                     /translation="NVIYSEYNVRVRIICGSVVTGIRSFMSSYDEAMKYLKESIGSYK
                     MMMKPEINMLRDELFQSIFLELKNEILANVGNYPRIMHVFDVFVQDTVSYKLSVSEVR
                     EHCFELASALYYAYIAASGEMADNRLSQLITALSMTNAAEACKATSKFITEILVHEEE
                     INHEIIHKAKAYINQHLADDISVTVLAEQNFMSVSYFSRLFKKVVGIGCNEYIVQKRI
                     KAAENLLLTTPLPTGEIALRVGYKNKNYFSLAFKRKTGMAPTELRETSHYSHECQASD
                     DDL"
BASE COUNT          310 a          238 c          185 g          347 t
ORIGIN      
        1 aggtattctt tttggtcaat tcctttcact ctttaaagta tacaggaagc tcctaaaata
       61 attaaactta ccgtaagctt atgttgtaat cttacaccag gcaggcataa ttgaaaatgt
      121 attaatgtca tatttacatg gaaaaatgtt gtttttatca gtcatacttt tattcatacc
      181 tgctcccata tttctttctg gcttcctccg tacatgcagt tatgaacgct ccctttgctg
      241 ttacaaatca tcatcagatg cctgacattc atgtgaatag tgtgaagttt cccttaattc
      301 agtcggtgcc attcctgttt tccgtttaaa cgcaagggaa aaataatttt tatttttgta
      361 tccgacgcgc agtgcaattt ctccggtagg aagcggcgtt gtcagcagaa gattttctgc
      421 cgctttgatc cttttttgta caatatattc attacagccg attccaacta cttttttaaa
      481 caatcttgaa aaatagctga ccgacatgaa attctgttcc gcaagcactg ttacggaaat
      541 atcgtctgct aaatgctgat tgatataggc cttggcttta tggatgatct catgattgat
      601 ctcttcttcg tggacaagta tttccgtaat aaatttggag gttgctttac acgcctccgc
      661 cgcattcgtc atagaaagag ccgtaatcag ctgagacagc ctgttatctg ccatttcgcc
      721 gcttgctgca atataggcat aataaagagc agatgccagt tcaaaacaat gctcccggac
      781 ctcagacaca gacagcttat aggaaaccgt atcctgaaca aaaacatcga acacatgcat
      841 aatacgcgga taattgccga cgttcgctaa aatttcattt ttcagttcca aaaaaataga
      901 ttgaaacagt tcatcccgca gcatattgat ttcgggcttc atcatcattt tataggaacc
      961 gatcgactct ttcaaatact tcatggcttc gtcataggaa ctcataaagc tcctgattcc
     1021 cgtcacaacg cttccgcata tgatcctgac gcgaacatta tattcagaat aaatcacatt
//
LOCUS       DAOSYE010000891         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_132116_length_1079_cov_2.781250, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000891 DAOSYE010000000
VERSION     DAOSYE010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132116_length_1079_cov_2.781250"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLU43_12415"
     CDS             <1..401
                     /locus_tag="PLU43_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO29261.1"
                     /translation="SIHDEEQLRAMVSDHNRAILNSFVNIYIEKEKIFFDSRAKLLLY
                     LIGITNSVRQACDQMALSYGKAWNILNELEENLEYAIVERKQGGSRGGNTRLTQKGLA
                     FISAYQEFEENVIDYSHKEFERLFEKQSLL"
     gene            527..>1079
                     /locus_tag="PLU43_12420"
     CDS             527..>1079
                     /locus_tag="PLU43_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO29262.1"
                     /translation="MYHLNINGKEYVTAHDKSLLDYLRDDLSLKAAKDGCSEGACGAC
                     TVLVDGKKVKACVQKLSRFEGKKIITLEGIPSDEMKVYEHCFAAAGAVQCGFCIPGMI
                     ISAKSLLDVNLNPTRQDVKQAIKGNFCRCTGYQKIEDAILMSAEYFREGRAVPGEPET
                     LHMNERAKRIDVAEKVYGTGRFAD"
BASE COUNT          340 a          181 c          298 g          260 t
ORIGIN      
        1 tcagtatcca tgacgaagaa cagctgcggg caatggtgag cgatcataac agggcgatcc
       61 ttaattcatt tgttaatatc tatattgaaa aagaaaagat tttctttgat tccagagcaa
      121 agctgctgct ttatctgatc ggcattacaa attcagtacg ccaggcgtgc gatcagatgg
      181 cgctttctta tggaaaggca tggaatattc ttaacgagct ggaagaaaat ctggagtatg
      241 cgatcgtaga aagaaagcag ggcggatcca ggggaggaaa tacaaggctt actcagaaag
      301 gacttgcttt tataagtgcg tatcaggaat ttgaagaaaa tgtcatcgac tattcccata
      361 aagagtttga gaggctgttt gaaaaacagt cactgctgta attttgtata aaaaagaaca
      421 ggaaagagcg tatacttgcg aagaagaagt tgtaagaatg cgcttttttt tatataattt
      481 aaaataatag gatattgaaa acaaaacaca gggggcagta gaggaaatgt atcatctgaa
      541 tattaacggc aaagagtatg taaccgcaca tgataagagc ctgctggatt atctgagaga
      601 tgatctttcg ctgaaggctg caaaggatgg atgcagcgaa ggcgcctgcg gcgcctgtac
      661 ggtccttgtg gacgggaaaa aggtaaaggc ctgtgtgcag aagctcagca ggtttgaagg
      721 aaagaagatc ataacgcttg aagggattcc ttccgatgaa atgaaagtat acgagcattg
      781 ttttgctgcg gcaggagcgg tacagtgcgg attttgtatt ccggggatga tcatatccgc
      841 caaaagtctg ctggatgtca atctgaatcc gaccagacag gacgtaaagc aggcgatcaa
      901 agggaatttt tgcaggtgta ccggatatca gaagatcgag gatgcgattc tgatgtctgc
      961 agaatatttc cgcgaaggca gggcggttcc cggggaaccg gaaacgcttc acatgaatga
     1021 gcgggccaag cggatcgatg tggcggaaaa agtatatgga acaggcaggt ttgccgatg
//
LOCUS       DAOSYE010000892         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_132586_length_1077_cov_2.024462, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000892 DAOSYE010000000
VERSION     DAOSYE010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132586_length_1077_cov_2.024462"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1077)
                     /locus_tag="PLU43_12425"
     CDS             complement(<1..>1077)
                     /locus_tag="PLU43_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO29263.1"
                     /translation="SSDDYKPVRSVLEKDAMHGGNVSCETKLLCRDGSLVEAEMSVRM
                     AVYENVRFYYVVLINLTKSRRKEEILKKELSKYRFRASRDALTGIYNQETFFSETRKL
                     VEKYPGERFVIGEWNIDRFKAINELFGSHAGDQIICAFADHLQRTYIEKCTYGRMESD
                     HFVTCCPESFLEERSPEVEALLNGQMSWHTLNHPISLHAGFYRVEQGERDVAIMCDRA
                     GMALQQIKDSFIQREKYFNDDLLEALVKEQQMMRDADAALNNKEFFVMYQPIIDVKTK
                     KIVAAEALVRWKKRTGEIVPPGEFIPIFERNGFISKLDMYVWEEVCRYQGQRKAVGKK
                     LVPISVNLSRIDFSARHGIPSVAQ"
BASE COUNT          273 a          291 c          195 g          318 t
ORIGIN      
        1 cctgcgcaac tgagggaatt ccgtgtctgg ctgaaaaatc aatacgggac agattgaccg
       61 atatcggaac gagttttttt ccgaccgcct tacgctgacc ctgataacgg cagacctctt
      121 cccatacata catatccagt tttgaaataa agccgtttct ttcaaatatg gggataaatt
      181 caccaggcgg aacgatctca ccggttctct ttttccagcg gacaagtgct tcggccgcaa
      241 cgattttttt cgttttaaca tcaatgatcg gctgatacat cacaaaaaat tctttattat
      301 ttaaggcagc atccgcatcg cgcatcatct gctgttcttt tacgagcgct tctaaaagat
      361 catcgttaaa atacttttct ctctggataa agctgtcctt gatctgctgc agcgccatac
      421 cggcacggtc gcacatgatc gcaacatccc gttctccctg ttccaccctg tagaatcccg
      481 catgcagcga aatcggatga ttcagtgtat gccaggacat ctgaccgttc aataacgcct
      541 ccacctctgg cgaacgctct tccaaaaaac tctccggaca gcatgtcaca aaatgatccg
      601 attccattct tccgtaagta catttctcaa tatatgtacg ttgcaggtgg tcggcaaatg
      661 cgcagatgat ctgatcgcct gcatgacttc caaataattc attgattgct ttaaaacgat
      721 cgatattcca ctctccgatc acaaaccgtt cgcccggata cttttccaca agctttctcg
      781 tttcactaaa aaacgtttcc tgattataaa tgcctgtcag ggcatctctg cttgcgcgga
      841 atcgatattt actcagttcc tttttcagaa tttcttcttt ccgcctgctt tttgtcagat
      901 ttataagaac cacataataa aatctgacat tctcatacac agccatccgg acactcattt
      961 ccgcttcaac aagactgccg tccctgcaga gaagttttgt ttcgcagctt acgtttccgc
     1021 cgtgcatcgc gtccttttcc aatacactcc tgaccggttt ataatcgtct gaggaga
//
LOCUS       DAOSYE010000893         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_132846_length_1076_cov_1.843291, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000893 DAOSYE010000000
VERSION     DAOSYE010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132846_length_1076_cov_1.843291"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLU43_12430"
     CDS             <1..819
                     /locus_tag="PLU43_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29264.1"
                     /translation="ADDSTDAADTVSENTLSENNLSAVEVVSDNSISDNSVSDNDISD
                     AAVLDDSVSGNSVSENSVSENELTVSLIRVALPVKLDFAMNPDGFGGMGQILSPDYDM
                     VNYSDVPIEVEISDIYYEFADPENCVSLSVPYDKDDSAIKGKKAFYLYLGHADLKEEG
                     DSVRKYYDEYIDGQDAAWGSQTFMEGSSASGDDILITDEHLAEPVTLILEPSVYDEDG
                     ELISTPTSAMASFRFFGSMSTSSDVKWFSDNVSLKVVYKYKAVKSAESVNEGEN"
     gene            822..>1076
                     /locus_tag="PLU43_12435"
     CDS             822..>1076
                     /locus_tag="PLU43_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29265.1"
                     /translation="MSNKKSVKIEFMEATNYLLKIIVSGYVLVLFLALPLYYNNRYYN
                     IGDIKFLLYKNLTWLLFILMAAVLLLQGVYFLADKIKNKEN"
BASE COUNT          329 a          172 c          243 g          332 t
ORIGIN      
        1 gcggacgata gcacggatgc tgccgatacg gtttctgaaa atacattgtc tgaaaataat
       61 ttatcggctg tggaagtcgt ttctgataac agtatttcag ataattctgt ttccgataat
      121 gatatttccg atgctgctgt tttagatgac agtgtttccg gtaattccgt atcagaaaat
      181 tccgtatcag aaaatgaact gacagtcagt ctgatcaggg ttgctcttcc tgttaaattg
      241 gattttgcca tgaatccgga cggctttgga ggaatgggac agattttgtc accggactat
      301 gacatggtca attacagcga cgttccgatc gaagtggaga tctcggatat ttattatgaa
      361 tttgcagatc cggaaaattg tgtttccctg tcagtgcctt atgacaaaga cgattcggca
      421 ataaaaggga aaaaagcatt ttatttatat cttgggcacg cagatctgaa agaggaggga
      481 gattccgtca gaaaatatta tgatgaatat attgacggtc aggatgctgc atggggcagt
      541 cagacgttta tggaaggaag cagtgcatca ggagacgata tactgattac ggacgaacat
      601 ttggctgaac cggtcacgct catactggag ccttctgttt atgacgaaga cggagaacta
      661 atatcgacac cgacttcggc aatggcttcc tttcggtttt tcggaagtat gagcacgagt
      721 tcggatgtaa aatggtttag tgataatgtc agcttaaagg ttgtatacaa atataaagcg
      781 gtaaaatccg cagaatctgt aaatgagggt gaaaattaat catgtcaaat aaaaaatccg
      841 ttaaaatcga atttatggag gcgacaaatt atctgttaaa aattatcgtt tccggatatg
      901 tgcttgtatt gtttctggca ctgcctcttt actacaataa tcggtattat aacatcggag
      961 atataaaatt tctgctgtat aaaaatctta catggctttt atttatttta atggctgctg
     1021 ttttactttt acaaggtgta tattttctgg ccgataaaat aaaaaataaa gaaaat
//
LOCUS       DAOSYE010000894         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_132922_length_1075_cov_3.368627, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000894 DAOSYE010000000
VERSION     DAOSYE010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132922_length_1075_cov_3.368627"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLU43_12440"
     CDS             complement(<1..684)
                     /locus_tag="PLU43_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOO29266.1"
                     /translation="MLVPMAEILKKARKGGYGVPAVAAVNELSARACVAAAEETESPL
                     IMLCGYGHNPDMNYFGRILNDMATKTYVPTALILDHSATYEEAIKGIYAGFNTIMVDR
                     SKLPYEENVAQVKELVRVAHAAGIGVEAELGHVGVGENYAVDGVSALTVPEEAVKYVE
                     ETGVDCIAVAIGTAHGVYKGEPKLRFDLLTELAEKIPVPLVLHGGSGTGDEKLAQACQ
                     MGISKVNIAN"
     gene            complement(708..>1075)
                     /locus_tag="PLU43_12445"
     CDS             complement(708..>1075)
                     /locus_tag="PLU43_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOO29267.1"
                     /translation="TNDLTQYSLAVDRGNESVAQLYSYYHPAVLRLIKASIFGAHKNG
                     IPCGMCGEAAGDLLMLPVLIGMGLDEYSMTASSIPEIKERVGKLSVKGCEELAEKVLA
                     CSSKTEVERELKTFQSYIK"
BASE COUNT          250 a          318 c          197 g          310 t
ORIGIN      
        1 attcgcaata ttgaccttgc tgatccccat ttggcatgcc tgggcgagct tttcatcacc
       61 cgtaccggat cctccgtgaa gaacaagcgg aacaggaatc ttttccgcca gctccgtaag
      121 cagatcaaac ctcagctttg gctctccttt atatacgcca tgcgcagtac cgatcgccac
      181 cgcaatacag tccaccccgg tctcctctac atattttacc gcttcttccg gcacagtcag
      241 agcagacact ccatcaactg cgtaattttc accgactcca acatgtccaa gctccgcttc
      301 cacgccgatc cccgctgcat gagccacacg gacaagctct ttgacctgtg caacattctc
      361 ttcatacggc agcttggaac ggtctaccat gatcgtatta aatcccgcat agatcccttt
      421 gattgcttct tcatatgtag cactgtgatc cagaatcagg gctgtcggca cataagtctt
      481 tgtcgccata tcatttaaaa ttcttccaaa ataattcata tcagggttat gtccataacc
      541 gcataacatg atcaacgggg attctgtctc ctcggctgct gccacacatg cccttgcgga
      601 caattcattg actgctgcaa ctgccggtac tccatagccg ccttttcttg ctttctttaa
      661 tatttccgcc attggtacta acatattcat ttctccttta ctgctattta ttttatataa
      721 gactgaaacg tcttcagttc tctttccact tcggttttgc tgctgcacgc cagcaccttt
      781 tcagccagct cttcacagcc ctttacggaa agctttccga cccgttcctt gatttccggt
      841 atggaagatg cagtcatact gtattcgtcc agtcccattc caatcaggac cggaagcatc
      901 agcagatcgc ctgctgcttc tccgcacatt ccgcatggaa taccattttt gtgtgcaccg
      961 aaaatcgatg cttttataag ccgcagaaca gccggatgat aataactgta aagctgtgcc
     1021 acagattcgt ttcctctgtc caccgcaaga gaatactggg tcagatcatt cgttc
//
LOCUS       DAOSYE010000895         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_133160_length_1074_cov_3.320903, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000895 DAOSYE010000000
VERSION     DAOSYE010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133160_length_1074_cov_3.320903"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1074
                     /locus_tag="PLU43_12450"
     CDS             165..>1074
                     /locus_tag="PLU43_12450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO29268.1"
                     /translation="MKKKIKKVFGINIEQEIRADFDKEIIRANNVRCKIVLIILCVPM
                     IPYVIHLFLTSDPELPANCLILFIELIGLYLLFFHENAEYIGTLHILIMVVCLAWSQY
                     VNYMNDQSAVNYMPYLIGLMGLAAVAYLRPLQSVVLFGLNHLVFLLISFHVIKDGTIL
                     LENSVNSTIAVLFSILFSISNYHSKLRHSVNKKLLTDLHEANAKLTEYVHKDVGTGIN
                     NRRALNERLDTEWENALIFQKPLSFMMIDIDFFKQYNDTYGHSRGDVCLREVAQCISD
                     ITKKFNAYVARFGGEEFSVLLPDMNEE"
BASE COUNT          348 a          166 c          230 g          330 t
ORIGIN      
        1 atctggccgt atataaaatt gtacacaata aaaaacaata agatagaaga atttggaatg
       61 tcaatggctt gtaataacag aatattcgag atatactaat atatggtttc aatattaaaa
      121 acgtagtcta actatttatg gtgacataat aagggatagt atagatgaaa aagaaaataa
      181 aaaaagtatt tggaataaat atagagcagg agatcagagc ggactttgac aaagaaatca
      241 tacgcgccaa taatgtccga tgtaaaatag tactgatcat attatgcgtt ccaatgattc
      301 cttatgtgat ccatttgttt ttgacttcgg atccggaact gcctgcaaat tgtctgatat
      361 tgtttataga attgattgga ttatacctgc ttttttttca tgaaaacgca gagtatatcg
      421 gaacgctcca tattttgatc atggtagtct gccttgcctg gagccagtat gtaaattata
      481 tgaatgatca gtctgcagtg aattatatgc cgtacctgat cgggttgatg ggactggctg
      541 ccgttgcata tctgcggccg ctgcaaagtg ttgtcttatt tggactgaat catcttgtat
      601 ttcttctgat cagcttccat gtgataaaag acggaacgat cctgctggag aatagtgtga
      661 attctacaat agcagtttta ttttccattc tcttttccat atccaactat cattcaaaac
      721 tgcgtcattc cgttaataag aaattgctga cagatctgca tgaagcgaat gcgaaactga
      781 cggaatatgt gcataaagat gtcgggaccg gaatcaataa cagaagggca ttaaatgaaa
      841 gactggatac tgaatgggaa aatgccttga tttttcaaaa accactttca tttatgatga
      901 tcgatattga tttttttaaa cagtataatg atacatatgg tcacagcagg ggagatgttt
      961 gtctgcgaga ggtcgcacag tgtatcagcg atattacgaa gaagtttaat gcctatgtag
     1021 ccaggttcgg aggagaggaa ttttcagtgt tactgccgga tatgaacgag gaag
//
LOCUS       DAOSYE010000896         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_133186_length_1074_cov_2.803729, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000896 DAOSYE010000000
VERSION     DAOSYE010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133186_length_1074_cov_2.803729"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLU43_12455"
     CDS             <1..519
                     /locus_tag="PLU43_12455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO29269.1"
                     /translation="KIIQYAKKLEHLATIDTLTQLQNRRGMMNHLRKLADSYSSTNYP
                     FSLVISDIDLFKKVNDTYGHDTGDYVLANLAVILKDFMRDKGRVARWGGEEFLFVFEG
                     KNGDYVFEELNKLKFLIEKSIFTYKDYSFSITMTFGLEEFDSNTGIEKTIEKADEKLY
                     QGKEHGRNCVVY"
     gene            complement(533..1024)
                     /locus_tag="PLU43_12460"
     CDS             complement(533..1024)
                     /locus_tag="PLU43_12460"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOO29270.1"
                     /translation="MNVIVAADKNWAIGNKGGLLVSIPNDHKMFREETTGKVIVYGRK
                     TLETFPGGQPLKNRTNILLSKNPEYSVKGAVVLHSTEELLEELKKYPSEDIYIIGGDS
                     IYKQLLPYCNTAYVTKIEYVYEADAYFPELDLLPEWQMTAESEEQTYFDLEYTFQRYE
                     RNS"
BASE COUNT          315 a          236 c          203 g          320 t
ORIGIN      
        1 aagatcatcc agtatgccaa aaagctggag caccttgcca cgatcgatac gctgacacag
       61 ctgcagaacc gccgcggcat gatgaatcac ctgagaaaac ttgcggacag ctactcctca
      121 acaaattatc cgttttcact tgtcataagc gatattgatc tgttcaaaaa agtaaacgac
      181 acctatgggc atgataccgg tgattacgta cttgcgaatc ttgccgttat tttaaaagat
      241 ttcatgagag ataaaggccg cgtggcgcgt tggggcggag aagaattttt atttgtattt
      301 gaaggaaaaa acggagatta tgtgtttgaa gaactcaata agctcaaatt tcttattgaa
      361 aaatccatct ttacctataa agattacagc ttcagtatta caatgacctt cggtcttgaa
      421 gaattcgatt ccaataccgg tattgaaaaa acaatagaaa aagcggatga aaagctttat
      481 cagggaaaag aacatggccg aaactgtgta gtttactaat caatgcagtc tgtcagctgt
      541 ttctttcata tcgctgaaag gtatattcca aatcaaaata tgtctgttct tcactttccg
      601 ccgtcatctg ccattccggc agcagatcga gctccggaaa ataagcgtcg gcttcatata
      661 catactctat cttcgtcaca tatgccgtat tgcagtatgg aagcagctgc ttataaatac
      721 tgtcgcctcc gataatgtaa atatcctcag aaggatattt ttttaattct tccaaaagtt
      781 cctccgtact atgcaggacg actgcgcctt tcacggaata ttccggattt tttgacaaga
      841 ggatattcgt ccgatttttg agcggctgcc cgcccggaaa tgtttcaagc gtttttctgc
      901 catacacaat gactttccct gtcgtttctt cccgaaacat tttatggtca tttggaatac
      961 tgacaagcag tcctccttta ttgccaatcg cccagttttt gtcggctgcc acaataacat
     1021 tcatataagc ttctcctttg atcacactct tattatatcc aaaaatactg cagt
//
LOCUS       DAOSYE010000897         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_133594_length_1072_cov_3.810226, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000897 DAOSYE010000000
VERSION     DAOSYE010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133594_length_1072_cov_3.810226"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLU43_12465"
     CDS             <1..142
                     /locus_tag="PLU43_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO29271.1"
                     /translation="QSLLEKIPLKRIGQPQDIANAFLYLASDLASYVTGAVLSVDGAM
                     RT"
     gene            complement(240..>1072)
                     /locus_tag="PLU43_12470"
     CDS             complement(240..>1072)
                     /locus_tag="PLU43_12470"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO29272.1"
                     /translation="CIPFNFPELRTKAKFAAIPSTSGTATEVTAFSVITDYEKGIKYP
                     LADFNITPDVAIVDPELVENLPIKQVAYTGMDALTHAIEAYVSTLHCTFTDPLALKAI
                     QIVNSDLLKSYEKDMSSREEMHYGQCLAGMAFSNALLGIVHSMAHKTGAAFSTGHITH
                     GLANAMYLPYVIAYNAKDEKAADRYVDIANTMGITGTKEECILALRKKIRSMNDALGI
                     PNTLKEFGIVEAEFKEKIAVISKNAVGDACTGSNPRTIDPAAMEKLFTCIYYGTEVDF
                     "
BASE COUNT          289 a          242 c          218 g          323 t
ORIGIN      
        1 acagagtctg cttgaaaaga tcccgttaaa gaggatcgga cagccgcagg atattgcaaa
       61 tgcgtttctt taccttgcca gtgatctggc cagctatgtg accggggccg tcttatccgt
      121 tgacggcgct atgagaacgt aaaacaattc aaaaaaacgg gtgtttccca agtgatgaaa
      181 cgacttggaa aacacccgtt ttttatatct tcgtacaaat ttttcttctt gaaatttatt
      241 taaaaatcaa cttccgttcc ataataaata caggtgaaaa gtttctccat tgcagccgga
      301 tcgatcgttc tcgggttaga gcctgtgcag gcatcgccaa ctgcattttt agaaatcact
      361 gcaatttttt ctttaaattc tgcttctacg attccaaatt ctttcagcgt attcggaatt
      421 cccagcgcat cattcatact gcggatcttt ttccggagtg caaggataca ttcttccttt
      481 gttcctgtaa ttcccatcgt attcgcaata tccacgtagc ggtctgccgc tttttcatcc
      541 tttgcattgt aagcaattac atatggcaga tacatggcat ttgcaagtcc gtgcgtaata
      601 tgtcccgtag aaaaggcagc tcctgtttta tgagccatgg aatgaacgat tccaagcaat
      661 gcattggaaa atgccatacc ggccagacac tgtccgtaat gcatttcctc tctgctcgac
      721 atatcctttt cataagattt caacagatca ctgttgacga tctggattgc ttttaacgca
      781 agcggatccg tgaatgtaca atgcaaggtg gaaacatacg cttcgatcgc atgagtcagt
      841 gcatccatac cggtataggc tacctgcttg atcggaagat tttcgaccag ctcaggatca
      901 acgatcgcta catccggtgt aatattaaag tcagccagcg gatatttaat tcctttttca
      961 taatccgtaa taacggaaaa agcggtcact tccgttgctg ttcctgaagt agatggaatt
     1021 gcggcaaact ttgcttttgt tctcaattcc ggaaaattga acgggatgca aa
//
LOCUS       DAOSYE010000898         1071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_133880_length_1071_cov_2.523622, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000898 DAOSYE010000000
VERSION     DAOSYE010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133880_length_1071_cov_2.523622"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..73)
                     /locus_tag="PLU43_12475"
     tRNA            complement(1..73)
                     /locus_tag="PLU43_12475"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Thr,seq:ggt)
     gene            complement(160..333)
                     /locus_tag="PLU43_12480"
     CDS             complement(160..333)
                     /locus_tag="PLU43_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase"
                     /protein_id="HOO29273.1"
                     /translation="MEAHAEYIVEKIDGDYAYLKRIDKDMDDLNQVARALLPPEITEG
                     STLIKEWFEYRIK"
     gene            complement(372..986)
                     /gene="sigH"
                     /locus_tag="PLU43_12485"
     CDS             complement(372..986)
                     /gene="sigH"
                     /locus_tag="PLU43_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOO29274.1"
                     /translation="MNNEKYMDIPDEELIVRLRDGEREITEFIMDKYKNLVKNKAKAM
                     FILGADTEDLIQEGMIGLFKALRDYDLGRDASFHTFADLCISRQIYTAVQASNRKKHM
                     PLNTYISLYSNIAESEGEEAYLVEALQSAADKSPEELMIDKENVAGLEKAIEQALSPF
                     EKQVLDLYVTGMSYVQIARILDRDEKSTDNALQRIKAKIRRVTE"
BASE COUNT          239 a          295 c          207 g          330 t
ORIGIN      
        1 tgctgacgat gagaatcgaa ctcatgacct cctcactacc aatgaggtgc gctaccgact
       61 gtgccacgtc agctttttgt ctgtaaatca catcgacgaa agtcatttta tcacactgtc
      121 cgatcgcttg tcaatgataa ttgtgatccg gccggccgct tattttatac ggtattcaaa
      181 ccattctttt atgagagtgc tgccctctgt aatttccggc ggcagcagcg cccgcgcaac
      241 ctggtttaag tcgtccatat ctttgtcaat gcgctttaaa tatgcataat caccgtcgat
      301 tttttccaca atatattccg catgggcttc catactgatg catcctttcc tgccgtccgg
      361 ctgttaggct gttactccgt tacacgcctg atttttgcct tgatcctctg cagtgcatta
      421 tccgtagatt tttcatcccg atcaagtata cgggcaatct gcacgtaact cattcccgta
      481 acatacagat caagcacctg cttttcaaag ggactgagtg cctgctcgat cgctttctcc
      541 agacctgcca cattttcttt gtcgatcata agctcttcag gacttttatc cgcagcagac
      601 tgcaatgctt ccactaaata agcttcttct ccttcgcttt ccgcaatatt gctgtacagg
      661 gaaatatacg tattcagcgg catatgcttt tttcgattgg atgcctgcac cgcagtatat
      721 atctgcctgg aaatgcacag atccgcaaat gtatggaagc tcgcatccct gccgaggtca
      781 taatcccgca gcgccttgaa aagcccgatc atcccttcct ggatcaggtc ttccgtatcg
      841 gctccaagaa taaacatcgc ctttgccttg ttttttacaa ggttcttata cttatccata
      901 ataaattctg tgatttcccg ttccccgtca cgcagtctga cgatcagctc ttcgtcggga
      961 atatccatat atttttcatt attcatatcg atgctccggg ctatggggct atgacgccct
     1021 gttccagtta atatccgcgc tgccgtacga tctcataggc aagcacgcct g
//
LOCUS       DAOSYE010000899         1070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_134128_length_1070_cov_2.066010, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000899 DAOSYE010000000
VERSION     DAOSYE010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134128_length_1070_cov_2.066010"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1070)
                     /locus_tag="PLU43_12490"
     CDS             complement(<1..>1070)
                     /locus_tag="PLU43_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOO29275.1"
                     /translation="AIVSLNNELRELELREVKEIEIILASLSVKCAEYLTKIRQDLET
                     LTELDFIFAKAFLAMEQNAVCPLYNTEGRVHLRKARHPLLDKKTVVPIDIRLGETFNL
                     LIITGPNTGGKTVALKTVGLLSLMGQSGLHIPTLDRSELPVFSEVFADIGDEQSIEQS
                     LSTFSSHMKTIIRILQNADENSLCLFDELGAGTDPTEGAALAIAILTELHARQIRTIA
                     TTHYSELKVFALKEPGVENACCEFDIETLRPTYRLLLGIPGKSNAFAISGKLGLPDLI
                     IQTARAQLGKQEKSFEDVLSELETSRSTIEREKEEIAHYKKEIEALKTEFETKQEKLN
                     ASRNKILREANEKARDILQNAK"
BASE COUNT          240 a          260 c          261 g          309 t
ORIGIN      
        1 tttggcattc tgcaaaatat cgcgtgcctt ttcgtttgcc tcccggagga tcttattgcg
       61 ggaagcattc agtttttcct gctttgtttc aaattcggtc ttcagcgctt cgatttcttt
      121 tttataatgt gcaatttctt ctttttcccg ttctattgtg cttcggctgg tttccagttc
      181 ggaaagaaca tcctcaaaac ttttttcctg tttgccaagc tgtgctctgg ctgtctgaat
      241 gatcagatca ggaagcccga gcttgccgga aatggcaaac gcattgcttt ttcccggaat
      301 gccaagcagc aggcggtagg taggccgaag cgtttcgata tcgaattcac agcaggcatt
      361 ttcaacgccg ggttctttta atgcaaaaac ctttagttca ctgtaatggg tcgtggcgat
      421 cgtgcggatc tgtcttgcat gcagctccgt taatatggca atggcgagcg cagcgccttc
      481 tgtcggatca gtgccggctc ccagctcgtc gaacagacaa agggaatttt catctgcatt
      541 ctgcaggatc cgaatgatcg tcttcatatg ggaagaaaaa gtggaaagac tctgttcgat
      601 gctctgttca tcgccgatat ccgcaaatac ttcggagaat acgggaagct ctgagcggtc
      661 cagcgtcgga atatgcagac cggactgtcc catcagggaa agcaggccga cagttttgag
      721 tgcaacagtc tttccgccgg tgttcgggcc cgtaataatg agcaggttaa aagtctctcc
      781 aagccggata tcgatcggta cgacggtttt tttatcaagc agcggatgcc ttgccttgcg
      841 cagatgaaca cggccctccg tattatacag cggacagacg gcgttctgtt ccatggcaag
      901 aaacgctttt gcaaatataa aatcaagttc agtcagcgtt tccagatcct gtctgatctt
      961 tgtcagatat tccgcgcatt ttacactgag agaggcaagt atgatctcga tctcctttac
     1021 ttcccgcagt tccagctccc gcagttcatt gttcaggctg acaatggcag
//
LOCUS       DAOSYE010000900         1069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_134264_length_1069_cov_3.249507, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000900 DAOSYE010000000
VERSION     DAOSYE010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134264_length_1069_cov_3.249507"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PLU43_12495"
     CDS             <1..865
                     /locus_tag="PLU43_12495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOO29276.1"
                     /translation="AQGIFVNFKNVQRTSRKKIPFGICQVGKSFRNEITPGNFIFRVR
                     EFEQMEMEFFCEPDTDLEWFAYWKQYCIDFLQKLGMKEEDMRVRDHDKEELSFYSKAT
                     TDIEFLFPFGWGELWGIADRTDYDLTQHQNVSGQDMSYFDDEKKAKYVPYVIEPSLGV
                     ERLFLAVLCGAYDEETIDSGDTRTVLHFHPALAPVKIGVLPLSKKLNEGAEKIFTALS
                     RKYHCEFDDRGNIGKRYRRLDEIGTPYCVTYDFDSETDQAVTVRDRDTMQQERIRIED
                     LEKYFEEKFVF"
BASE COUNT          314 a          163 c          284 g          308 t
ORIGIN      
        1 agcacagggt atttttgtga attttaaaaa cgtacagaga acttccagaa aaaagattcc
       61 gtttggtatt tgtcaggtcg gaaagtcttt tcgaaatgag attacaccgg ggaactttat
      121 tttccgtgtc agggaatttg agcagatgga aatggaattt ttctgtgagc cggatacaga
      181 tctggaatgg tttgcatact ggaagcagta ttgtattgat ttcttgcaga aacttggtat
      241 gaaggaagag gatatgcgtg tcagggatca tgataaagaa gaactttcct tctattcaaa
      301 agctacgaca gatattgagt ttttgtttcc gttcggctgg ggagagcttt ggggaattgc
      361 ggacaggacc gattatgatt taacgcagca ccagaatgta tcagggcagg acatgagcta
      421 ctttgatgat gagaagaaag caaaatatgt gccttatgtt attgagccgt cgcttggtgt
      481 ggaaagatta ttccttgcgg ttttgtgcgg cgcttatgat gaggaaacga tcgacagcgg
      541 ggataccagg acagtgcttc attttcatcc ggcactcgca ccggtcaaga tcggcgtact
      601 gccgttgtcc aaaaagctga atgagggagc ggaaaagata tttacggcgc tttccagaaa
      661 atatcattgt gagtttgacg acaggggaaa cattggaaag agataccgca gactggacga
      721 gatcggaaca ccgtactgtg ttacgtatga ttttgattct gaaacggatc aggcggttac
      781 tgtgcgcgac agggatacga tgcagcagga aagaatcagg atcgaagacc tggagaaata
      841 tttcgaagaa aagtttgttt tttaatgtac tttaaaccat aatatgccgg ggtatcattg
      901 gatactccgg tttcttaata ttatagtttt attttattgg acgcggtcgt tgaaattagc
      961 gtgaagatat gttaaaatca agtttagttt taatttcgga ggcaaaaata tggcacagtt
     1021 atggggcgga agatttacaa aagaaacgga tgcgttggta tatcatttt
//
LOCUS       DAOSYE010000901         1069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_134292_length_1069_cov_2.720907, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000901 DAOSYE010000000
VERSION     DAOSYE010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134292_length_1069_cov_2.720907"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..915)
                     /locus_tag="PLU43_12500"
     CDS             complement(61..915)
                     /locus_tag="PLU43_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29277.1"
                     /translation="MNLTKKSLPDKFGIQTNDINVHTKSLPFYVKECGYSNERKFTVG
                     NKNEYSDILLLYCVSGIVRFDKHKTSHFLHPDSLVVSACSSPLTFIRASKEWSFLYVI
                     VSGSHAKQFYNNIRNNSSVHTANPLSCLLDQFIDLYNLHYSGDLLPNMEASLLIHHIF
                     CELYKLSLSIMDTKSLSPVQESVVNTALKYIGQNYMNFLDIDTICNEVSFSKYYFCKL
                     FKKQVGVSVHQYVSEFRITKAKELLTYSKLSIGAIATTVGYKNTLAFSRAFERSTHMT
                     PTEFRRYY"
BASE COUNT          344 a          198 c          247 g          280 t
ORIGIN      
        1 ggaatccatc aaactcaagt aaggctgcag ctgcaaaaag ggagagagcg gcacctgcct
       61 ttagtaatat ctgcggaatt cggtcggcgt catatgggtg gaacgctcaa atgcgcggct
      121 gaacgcgagt gtatttttat aaccgacagt agttgcaatt gcaccgatag agagcttcga
      181 gtaagtcaga agctcttttg cttttgtaat acggaattca ctgacatatt gatggacgga
      241 aacgccgacc tgttttttga aaagcttgca gaaatagtat ttggaaaagc tgacttcatt
      301 gcagatcgta tcgatatcca gaaagttcat ataattctgc ccaatatatt tcagggcggt
      361 attcacaaca ctttcctgca caggagacag tgacttggta tccatgatac ttagggaaag
      421 cttatagagc tcacagaaaa tatgatggat cagcaggctt gcctccatgt tcggaagcag
      481 gtctcctgaa taatgcagat tatacaggtc aatgaactgg tccagcagac agctgagcgg
      541 attggccgta tgaacgctgg aattgttcct gatattatta tagaactgct ttgcatggct
      601 gccgcttacg atgacataga ggaacgacca ttccttgctg gctctgataa aagtaagcgg
      661 ggagctgcag gcggatacga caaggctgtc cggatgcagg aaatgggaag ttttatgctt
      721 gtcaaaacga acgatcccgg atacgcagta cagcagaaga atatcggaat attcgttttt
      781 gtttccaacc gtaaattttc gttcgtttga atagccgcat tccttgacat agaaaggaag
      841 gctttttgta tggacattaa tatcgtttgt ttggatgccg aatttatccg gcagactttt
      901 tttggtcaaa ttcacaatga ggcctccgaa attgaaaaga aaaatataaa aaataaataa
      961 tattccggca aaagtccgga gagaatataa aattacacta cgaatcatac caaaaaacaa
     1021 aaaaaatagc aatcttgtat gaaaaagcaa gaaaagatat tgaaaaagc
//
LOCUS       DAOSYE010000902         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_134449_length_1068_cov_3.577493, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000902 DAOSYE010000000
VERSION     DAOSYE010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134449_length_1068_cov_3.577493"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLU43_12505"
     CDS             complement(<1..269)
                     /locus_tag="PLU43_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29278.1"
                     /translation="MINSNLLNQLRKISPEEQKLLDGEKNVQKNIYTNEQDFVVDSHK
                     MLARGKLIDIRLHTRFVHFPAHRHNYIEITYMCCGSTTHIINGRT"
     gene            413..>1068
                     /locus_tag="PLU43_12510"
     CDS             413..>1068
                     /locus_tag="PLU43_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOO29279.1"
                     /translation="MGDYFLAVDIGASSGRHILGTIKDGKMVIEEIYRFENGMIKKHG
                     HLYWDAAALANEIIAGMKKCKEIGKIPVTMGIDTWGVDYVLLDSAGEIAGNVYGYRDS
                     RTQGMDEEVCRLISPEELYMRSGIQKMPINTVYQLMAAKLAEPEVLKRAETFLMMPDY
                     FNYVLTGIKKSEYTNATTGQLVNAAAKDWDRELLDRLGLPEKLFLPLTMPGTDVGELK
                     P"
BASE COUNT          296 a          203 c          299 g          270 t
ORIGIN      
        1 tttgttctgc cgttgatgat gtgtgtggtg gagccgcagc acatataggt gatctcgata
       61 taattgtgcc ggtgggccgg aaagtgtacg aaacgggtat gcagacggat atcgatcagc
      121 tttccgcgcg ccagcatctt atggctgtca acaacaaaat cctgctcgtt tgtatagatg
      181 tttttttgga cgtttttttc accatccagg agtttctgct cctcagggga tatttttcta
      241 agctggttca acaagttgga atttatcatt ttgctcacct ctttctacag tttagcacag
      301 aaagggttcc gctgcaaata aggacggtaa attatgcaac tgccgtccta aaaatatact
      361 gctgcaaaga atatactaat agatagaaag tctgtgaaag gagaggacag gtatggggga
      421 ttattttttg gcggtagata ttggtgcctc gagcggacgc catattctag gaacgatcaa
      481 agacgggaaa atggtgatcg aggaaatcta ccgttttgaa aacggcatga taaaaaagca
      541 cggacatctg tattgggatg ctgcggcgct tgcaaacgag atcattgccg gaatgaaaaa
      601 atgcaaagag atcggaaaga taccggtcac aatgggaatc gatacatggg gtgtcgacta
      661 tgtgctgctg gattctgccg gggaaattgc aggtaatgta tatggttacc gtgacagccg
      721 tacacagggg atggatgaag aggtctgccg tctgatctcg ccggaagagc tctatatgag
      781 aagcgggatc cagaaaatgc cgatcaatac ggtctatcag ctgatggcgg caaagcttgc
      841 ggagcctgaa gttctgaaac gtgcggaaac gttcctgatg atgccggatt attttaatta
      901 cgtgctgaca gggatcaaaa agagcgagta tacaaatgca acgacagggc agttagtcaa
      961 tgcggctgca aaagactggg accgggagct gcttgacagg cttggacttc ctgaaaagct
     1021 gtttctgcct ctgacgatgc cgggaacgga tgtcggagaa ttgaagcc
//
LOCUS       DAOSYE010000903         1067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_134713_length_1067_cov_2.913043, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000903 DAOSYE010000000
VERSION     DAOSYE010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134713_length_1067_cov_2.913043"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLU43_12515"
     CDS             complement(<1..360)
                     /locus_tag="PLU43_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOO29280.1"
                     /translation="MNDHNNFTENTKKNKHFLHIAVLCMILTTATLCGCQADISGQHT
                     PISKSGFALDTFITVTIYDSTDTDLLDTCFSLCDTYEQLFSRTITGSDIDRINQAGGS
                     LTTVSGDTFSILQKSVYY"
     gene            473..>1067
                     /locus_tag="PLU43_12520"
     CDS             473..>1067
                     /locus_tag="PLU43_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29281.1"
                     /translation="MMANSFYNNDPEDSGAYRKMVMLCIAAASVVMLLFLVVLYVNSN
                     NKKTDTADTAAVESEESDEETEEESKIGKSNLTSSDLDFWDMYDEEADDEQLPEEEEP
                     AGVSAEFTPEKKTDEEDENKEEKDSMTKTPEEEPAADDEMNDGKHIAIADEDGNKVWY
                     EILEDVPKNTYKLDEYLKQDGEKLTYTESGKSSAFGID"
BASE COUNT          325 a          175 c          274 g          293 t
ORIGIN      
        1 gtaatagaca cttttttgca gtattgaaaa ggtatcgccg ctgacagttg ttaaagaacc
       61 gcctgcctgg ttgatccgat cgatatcgct gcctgttatt gtgcggctga acagctgttc
      121 ataagtatca caaagcgaaa aacatgtgtc aagcaggtct gtgtcagtgc tgtcatagat
      181 cgtgaccgta ataaacgtat ccagagcaaa tccggatttt gaaataggcg tatgctgtcc
      241 ggatatatcg gcctggcagc cgcagagcgt agcggttgtc agtatcatgc aaagtacggc
      301 aatatgcaga aaatgtttgt tttttttcgt gttttcagta aaattgttat gatcgttcat
      361 gctatagttc ctttcactga tcttatcaca ccagccattt tgtgacaaga aagatattta
      421 attttattgg tttttctgtt atactttttg taaaaataat agatggggtt ttatgatggc
      481 taattcattt tataataatg atcctgaaga ttccggggca tacaggaaga tggtcatgct
      541 ttgtattgcg gcagcttctg ttgttatgct gctgttcctt gtcgtattgt atgtgaattc
      601 caataacaaa aagaccgata cggctgatac tgcggctgtt gaatccgaag aatcggatga
      661 agagacggag gaggaatcaa agatcgggaa aagtaatctg acttcttctg atttggattt
      721 ttgggatatg tatgatgagg aggcagacga tgagcagctt ccggaagagg aggaaccggc
      781 aggtgtgtcc gccgagttta cgcctgaaaa gaagacggac gaagaagatg aaaataaaga
      841 agaaaaagac agtatgacaa agacgcctga ggaggaaccg gctgcagacg atgagatgaa
      901 tgacggaaag catattgcga tcgcagatga ggacggcaat aaagtatggt atgaaatact
      961 ggaagatgtg cctaagaata cttataagct cgatgaatat ctgaaacaag acggagaaaa
     1021 gctgacatat accgagtcag gaaaaagctc ggcatttggg atcgatg
//
LOCUS       DAOSYE010000904         1064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_135332_length_1064_cov_3.094153, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000904 DAOSYE010000000
VERSION     DAOSYE010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135332_length_1064_cov_3.094153"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLU43_12525"
     CDS             <1..645
                     /locus_tag="PLU43_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOO29282.1"
                     /translation="GPGYLGVAFHTAGLIELEAVVDMPGEFSVYNALAAIAVCRHFQI
                     QKEDITKALKIAKVRGRIEMIPISEEFTLMIDYAHNAMALESLLVTLKKYDPKRLVCL
                     FGCGGNRSRLRRYEMGEVSGKLADLTIITSDNPRFEKPADIIADIRTGIKKTTGAYIE
                     IPDRKEAIAYAIHHAKEGDVIVLAGKGHEDYQEIEGKKYPMDERVLIREILEEV"
     gene            complement(642..>1064)
                     /locus_tag="PLU43_12530"
     CDS             complement(642..>1064)
                     /locus_tag="PLU43_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOO29283.1"
                     /translation="VIASLHTASCRPGTEEENTLAYIYAMYHPCVKIIGHADDGNFPV
                     DYRELVLFAKEKDVLFEINNASLLPCSYRLNARANCITLLSYCMEFHQPVILSSDSHG
                     KAQIGDFDSANALLKELSFPESLLISDAAQLFRYLHIT"
BASE COUNT          298 a          209 c          296 g          261 t
ORIGIN      
        1 ggtccgggct atctcggcgt tgcttttcac acggcgggcc tgatcgagct ggaggcagtc
       61 gttgatatgc ccggggaatt cagcgtgtat aatgcgctgg cagctattgc ggtgtgcagg
      121 cattttcaaa ttcagaaaga agatattacg aaagcactta aaattgcaaa ggtcagggga
      181 aggatcgaga tgatcccgat ctctgaggaa tttacactga tgatcgacta tgcacataat
      241 gcgatggcgc ttgagagtct tttggtgacg cttaaaaaat atgatccgaa gcgtcttgtc
      301 tgtctgtttg gctgcggagg caaccgctcc agactccgcc gttatgagat gggagaggtg
      361 tcgggaaagc ttgcggacct gacgatcatt acgtccgaca acccccgttt tgaaaaaccg
      421 gcggacatta ttgcggacat tagaacaggt atcaaaaaga cgacaggtgc ttatattgag
      481 attccggaca gaaaggaagc gatcgcctat gccattcatc atgcaaaaga gggggatgtg
      541 atcgtgcttg ccggaaaagg acatgaggat tatcaggaga tcgaagggaa aaaatatccg
      601 atggatgagc gggtattgat acgggagatc cttgaagagg tctaggtaat atgcaaatac
      661 cggaacagct gtgccgcatc ggaaataagc aggctttccg ggaaggaaag ttcttttaga
      721 agcgcattgg cagagtcaaa atctccgatt tgcgcttttc cgtggctgtc acttgataaa
      781 atgacaggct gatgaaattc catacaataa gataaaagag ttatacagtt tgcccgtgca
      841 ttgagccgat aagagcaggg cagcagggat gcgttgttta tttcaaacag cacatccttt
      901 tcttttgcga aaagaacgag ttctctgtaa tcaacgggga aattgccgtc atcggcatgt
      961 ccgatgattt ttacgcatgg atgatacatg gcataaatat aagcaagtgt attttcttct
     1021 tccgtaccgg gccggcagga ggcagtgtgc agggaagcaa tcac
//
LOCUS       DAOSYE010000905         1064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_135402_length_1064_cov_2.235877, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000905 DAOSYE010000000
VERSION     DAOSYE010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135402_length_1064_cov_2.235877"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1064
                     /locus_tag="PLU43_12535"
     CDS             <1..>1064
                     /locus_tag="PLU43_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO29284.1"
                     /translation="DKFWNRVIFPIMDVNHRIIGFGGRVMGQGEPKYLNSPETPIFDK
                     GRNLYGLNHARTSRKKNMILCEGYMDVIALHQAGFGQAVASLGTAFTSGQANLLRRYT
                     DEVLLTYDSDDAGVRAALRAIPILREAGITAKVIDMRPYKDPDEFIKNMGAQAFEERI
                     DAAENSFYYEVRMLEADYDFKDPESKTKFGGEVAKKLLRFTEELERDNYIEGIAEKYH
                     MGFENLRKLVGKYALQGEGIRQYERPKSGISSKKSPDEVALKTQRLLLTWLTEEPQIY
                     PLIRTYVTADDFTEVLYCKVAAMLFEQLKADKLDPAGIISRFEDEKEQREVASLFNTK
                     LEEVESLAEREKALKELLVK"
BASE COUNT          291 a          209 c          322 g          242 t
ORIGIN      
        1 cggataaatt ctggaaccgt gttatttttc cgattatgga cgttaatcac cggatcatcg
       61 gtttcggcgg cagggtcatg ggccagggcg agcccaaata cctgaattca ccggagacgc
      121 cgatatttga taaaggccgc aatctgtacg gactgaatca tgcgcgtact tcgcgcaaaa
      181 aaaatatgat tctgtgtgaa ggctatatgg atgtgatcgc gcttcatcag gcaggctttg
      241 gccaggcagt ggcatcgctt gggacggcat ttacttcagg gcaggccaat ctgctcagac
      301 gctatacgga tgaggtgctt ctgacttatg acagtgatga tgccggtgtc agggctgcgc
      361 tgcgggccat accgatttta cgggaagccg ggatcacggc aaaggtcatt gatatgcggc
      421 cgtataagga tccggatgag tttattaaaa atatgggagc acaggcattt gaggagcgga
      481 tcgatgcggc ggaaaacagc ttttattatg aggtcagaat gcttgaggcg gactatgatt
      541 ttaaggatcc ggaaagcaag acaaaattcg gtggcgaagt ggctaaaaaa ctgctgcggt
      601 ttacggaaga actggagcgg gataattata tcgagggaat tgcggaaaaa taccatatgg
      661 gatttgagaa tctcagaaag ctggtcggaa agtacgcgct gcagggagaa ggtattcggc
      721 agtatgaacg gcccaaatcc ggtatcagca gcaagaagag cccggacgag gtggctctga
      781 aaacacaaag gcttctgttg acatggctga cggaagaacc gcagatctat ccgttgatcc
      841 ggacatatgt gactgcggat gattttacgg aagtactgta ttgtaaggtc gccgccatgc
      901 tgtttgaaca gctgaaggca gataagctgg atcctgcggg cattatcagc cgctttgaag
      961 acgagaagga gcagcgggag gtcgcttccc tgtttaatac gaagcttgaa gaagtagagt
     1021 ctttggcaga acgggagaaa gcactgaagg aactgcttgt caag
//
LOCUS       DAOSYE010000906         1063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_135548_length_1063_cov_3.410714, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000906 DAOSYE010000000
VERSION     DAOSYE010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135548_length_1063_cov_3.410714"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLU43_12540"
     CDS             <1..196
                     /locus_tag="PLU43_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO29285.1"
                     /translation="RAYSLQFTKRAMLSRGAAGIRKKTLIVNLPGSPKAVKECLEYIL
                     PELEHGIKILTGEAHDCARS"
     gene            209..637
                     /locus_tag="PLU43_12545"
     CDS             209..637
                     /locus_tag="PLU43_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOO29286.1"
                     /translation="MGIIRAVCISKKRGTVKQNIKTCKVIENSGLENDAHAGDWHRQV
                     SLLSYEKIEEFKKTGAPVTDGTFGENLIVEGIDLPRLPIGTKLQCNEVVLEVTQIGKK
                     CHSGCEIYQLVGDCIMPREGIFAKVLHGGTISAGDEIKCI"
     gene            complement(671..>1063)
                     /locus_tag="PLU43_12550"
     CDS             complement(671..>1063)
                     /locus_tag="PLU43_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO29287.1"
                     /translation="IEILVELSNVFARSVLGESIFGISTNANKKMQLLLSYLHSSYHK
                     KITGESIENALETNFDYLNRLFKKCMGITIFQYLNTLRINRAKELLVATDMKLYEIAP
                     LVGFKDEFYFSKLFKKYTGVSPSSYAKL"
BASE COUNT          333 a          196 c          275 g          259 t
ORIGIN      
        1 gcgggcatac agcctgcagt ttacaaagcg tgcgatgtta agcaggggcg cagcaggtat
       61 ccggaaaaag acactgatcg tcaatctgcc gggaagtccg aaagcagtaa aagaatgcct
      121 ggaatatatt ttgccggaat tagagcatgg aataaagata ttaacaggag aagcacatga
      181 ttgtgcgcga tcctgaaagg ggacaattat gggaatcata cgggcagtct gtatcagtaa
      241 aaaaagaggg actgtaaaac aaaatataaa gacctgcaag gtcattgaaa acagcggtct
      301 ggaaaacgac gcacatgcag gggactggca cagacaggta agcctgcttt cctatgaaaa
      361 aatagaggaa ttcaaaaaga cgggtgcgcc ggttaccgac gggacattcg gagagaatct
      421 gatcgtagaa ggcatcgacc tgccgcggct tccgatcgga acaaagctgc aatgcaatga
      481 agttgttctg gaggtgacac agatcggaaa gaaatgtcac agcggctgtg agatctatca
      541 gctggtcggg gactgtatta tgccgaggga agggatcttc gcaaaagtac tgcacggcgg
      601 tacgatttct gcaggagatg aaattaagtg tatctaaagg ctgttcctct gcggcagact
      661 ggaaaaacgg ttacagtttt gcataagagg atggagacac gcccgtatat tttttaaaga
      721 gcttactgaa ataaaattcg tccttaaaac cgacaagcgg ggcgatttcg tataatttca
      781 tatcggtggc aaccaaaagc tccttggcac gattgatcct taatgtattt aaatactgga
      841 agatcgttat tcccatgcat tttttaaaca ggcggttcag ataatcaaaa tttgtttcaa
      901 gggcgttctc gattgattct ccagtgattt ttttatgata ggaagagtgc aggtagctta
      961 aaagcagctg catttttttg ttggcatttg tgctgatccc aaaaatcgat tctccgagta
     1021 cggaacgggc aaatacattt gataattcta caaggatctc aat
//
LOCUS       DAOSYE010000907         1063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_135619_length_1063_cov_2.259921, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000907 DAOSYE010000000
VERSION     DAOSYE010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135619_length_1063_cov_2.259921"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLU43_12555"
     CDS             complement(<1..382)
                     /locus_tag="PLU43_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29288.1"
                     /translation="MEDYSNDFSKFVQNAVDKAKELGGIAKLHAQIKAEETKKQEQYY
                     KLGKKYYELYKDAPEAELEEYINKLAACDEKIREYRKELKKEQNAGYRDVYKAAAPEE
                     EAAKASEEESYDGVEEVTNNEKCDK"
     gene            complement(447..>1063)
                     /gene="holA"
                     /locus_tag="PLU43_12560"
     CDS             complement(447..>1063)
                     /gene="holA"
                     /locus_tag="PLU43_12560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO29289.1"
                     /translation="SGRAVEFARQEEAVLKRWILGQIKKEGKQISAGALQLFLQRTGN
                     DMENIASELEKLLCYTMTGDAVTTADVEAVCTVLITNRIFEMINAVADKRQKEALRLY
                     YDLLMLKEPPLKILSLLARQFNLLMQVKQLAGSGAALAVIAEKTGLHGFVVRKYIVQA
                     GKLSEKTLRNAVEECIELENAVKSGRLKDVMSVELVLMKYSQAG"
BASE COUNT          225 a          309 c          166 g          363 t
ORIGIN      
        1 atttatcaca cttctcgttg tttgtgactt cctcaactcc atcatacgat tcctcttctg
       61 atgccttggc tgcctcttcc tccggagctg cggctttata tacatcacgg tatcccgcat
      121 tctgctcttt cttcagctct tttctatatt ccctgatctt ttcgtcacag gcagcaagct
      181 tatttatata ttcctcgagt tctgcctccg gcgcgtcttt atacaattca taatatttct
      241 tcccaagttt gtaatactgc tcctgctttt tcgtttcctc agcctttatc tgcgcatgca
      301 gtttggcgat cccgccaagt tcctttgctt tatctaccgc attctgtaca aattttgaaa
      361 aatcatttga ataatcttcc atcttaattc ctccttgtca tgtatttaat atttttttaa
      421 ttgtcacgtc tgcaatattc cggatttcaa cctgcctgcg aatatttcat gagcaccagt
      481 tccacactca tgacgtcctt caatctgccg gatttgaccg cattttccag ctctatgcat
      541 tcttccacgg catttctcag tgtcttttcc gaaagcttgc ctgcctgtac aatatatttc
      601 cttacaacaa agccatgcaa tcccgttttt tcagcgatca ccgccaaggc ggcaccgctt
      661 cccgcaagct gctttacctg catcaacagg ttaaactgcc gggcaagcaa agacagaatc
      721 ttcaagggcg gctctttcag catcagcaga tcataataaa gccgcaacgc ctctttttgt
      781 ctcttgtctg ctaccgcatt tatcatctca aaaatacggt ttgttatcag aaccgtacag
      841 accgcttcca catccgctgt tgtgactgca tctcctgtca tcgtatagca gagcagcttt
      901 tccaattcac ttgctatatt ctccatgtca tttcccgtac gctgtaaaaa cagctgcagc
      961 gctccggccg atatctgctt tccctccttt ttgatctgtc ctaaaatcca tcttttcagg
     1021 acagcctcct cctgtcttgc aaactcaact gctctgccgc tgt
//
LOCUS       DAOSYE010000908         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_137096_length_1056_cov_3.600400, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000908 DAOSYE010000000
VERSION     DAOSYE010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137096_length_1056_cov_3.600400"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..553
                     /locus_tag="PLU43_12565"
     CDS             47..553
                     /locus_tag="PLU43_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO29290.1"
                     /translation="MTLDIKSVTLFINESQYQFSIGDMYGQVMPSETLSHTLRKRLGL
                     TGTKESCNQGACGCCTVIMDGDAVASCMLLTVDCDGKHITTIEGLKDPVTGKLDPVQQ
                     AFLDHYAFQCGFCTPGIIMTTKALLDKNPHPSKEEITEALSGNYCRCISHYHVVEAVE
                     DLAGKERE"
     gene            555..>1056
                     /locus_tag="PLU43_12570"
     CDS             555..>1056
                     /locus_tag="PLU43_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOO29291.1"
                     /translation="MFQTPKDPKDYKYVGKSTPRKDAVDIVTGKAVFLDDFNVADQLI
                     GKSLKSPYSHAMIKSIHTEKAKALPGIKAVLTYEDCNQDWKMGWPPQKPILDRHLRYI
                     GDSVALVAGETEEICNEALDLIEVEYDVLPAVYDGLSALEEDAPQLYDEFKNNIVTPG
                     YPPFQQD"
BASE COUNT          325 a          187 c          254 g          290 t
ORIGIN      
        1 ctctgaaaga aaaccactga attttaattt gaaattggtt aactaaatga cattggatat
       61 taaatcggtg actttattta ttaatgaaag tcagtatcag ttttctattg gcgatatgta
      121 tggacaggtt atgccgagtg aaactctttc gcatacactt cgtaaaagac ttggtctgac
      181 aggaacaaag gagtcatgta accagggcgc atgcggctgc tgcacagtta ttatggatgg
      241 agatgcagtg gcatcctgta tgcttcttac cgtagactgt gatggaaagc atattactac
      301 aattgaagga ttaaaggatc cggttaccgg aaagcttgat ccggttcagc aggcatttct
      361 tgatcactat gcattccagt gcggattctg tacccctgga attattatga ctacaaaagc
      421 acttttggat aaaaatccgc atccatcgaa agaggagatt acggaagcac ttagcggaaa
      481 ctattgccgc tgtatcagcc attatcatgt tgtggaagcg gtggaagatc ttgccggaaa
      541 ggagcgtgaa taggatgttt caaactccaa aagacccaaa agattataaa tatgtaggaa
      601 aaagtacgcc tcgtaaagat gctgttgata ttgttacagg aaaagctgta ttcctggatg
      661 attttaatgt ggcagatcaa ttgattggta aatcattgaa atccccatat tcacatgcga
      721 tgatcaaatc cattcatacg gagaaggcca aagcattgcc tggaataaag gcagttttaa
      781 cttacgaaga ttgtaatcag gattggaaaa tgggctggcc gccgcagaaa ccgattttgg
      841 acaggcatct tcgttatatt ggagacagtg tggcattagt tgccggagaa acagaggaga
      901 tttgcaacga agcactggac ttgatcgaag tggaatatga tgttctgccg gctgtctatg
      961 acggattatc tgcgttggaa gaggatgcgc cgcagttata cgacgaattt aaaaataata
     1021 ttgtaactcc cggatatccg cctttccagc aggacg
//
LOCUS       DAOSYE010000909         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_137107_length_1056_cov_3.358641, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000909 DAOSYE010000000
VERSION     DAOSYE010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137107_length_1056_cov_3.358641"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLU43_12575"
     CDS             <1..409
                     /locus_tag="PLU43_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOO29292.1"
                     /translation="TARALRPVEADVSPQKKLSNTVRKNRERASHMNPAYVVFLTLAM
                     LVTGYVCIQYIQLQSNITNSVKQISVLESQLNDLKAENDDTESRIKDSVSLEEIKQRA
                     MDELGMQYAKEDQIVKYDSDDTDYVRQYIDVDE"
     gene            458..>1056
                     /locus_tag="PLU43_12580"
     CDS             458..>1056
                     /locus_tag="PLU43_12580"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HOO29293.1"
                     /translation="MRKQNHREETTQRFSIKMQKKLAVLFFLILVAFISLNVRLYVIN
                     RDNGEQYKKQVLSQQQYDSVTLPAKRGEIVDSNGSKIAVSQKVYNVIIDSKTLNSEDG
                     KYLEPTLSALFSTQIDFNYTKSEIRNYITENPSSQYRIVAKKQEYDTVSPLIDILNNT
                     EENPNVDGIWLEDTYQREYPYNSLACDVIGFVQGDNEGA"
BASE COUNT          349 a          184 c          255 g          268 t
ORIGIN      
        1 tactgcacgt gcgctgcgtc ctgtggaagc ggatgtctct ccgcaaaaaa agctcagcaa
       61 taccgtcaga aagaacaggg aacgtgcttc tcatatgaat ccggcttatg ttgtatttct
      121 gacactggcg atgttagtaa caggatatgt atgtatccag tatatacagc tgcagtccaa
      181 tattacaaac agtgtcaagc agatttcagt attggaaagt caactgaacg atctgaaggc
      241 ggagaatgac gatacggaaa gccgtattaa ggattccgtc agcttggaag agatcaagca
      301 gagagcaatg gatgaactcg gcatgcaata tgcaaaagag gatcagatcg taaagtatga
      361 cagcgatgat acggattatg tcagacaata tatagatgtc gatgaataac tgattttccg
      421 gaaatttgat tgatcaggga caggcaggtg tggatttttg agaaaacaga atcatcggga
      481 agaaacgaca caacggtttt ccataaagat gcagaaaaag ctggcagtat tattttttct
      541 aatactggtg gcttttattt ctttgaatgt acgtctttat gtaatcaata gagataatgg
      601 agagcaatac aaaaagcagg tgctttcaca gcagcagtat gacagcgtta cgcttccggc
      661 caaacgggga gagatcgtgg attcgaatgg ttcaaaaata gcagtcagcc aaaaggttta
      721 taatgtcatt attgattcga aaacactgaa ttccgaagac ggaaagtatt tggaaccgac
      781 gctttccgct cttttttcga ctcagatcga ctttaattat acaaaaagcg agatccgaaa
      841 ttatattacg gaaaatccga gttcacagta tcggatcgta gcgaaaaagc aggagtatga
      901 tacggtcagc ccgctcatag atatcctgaa caatacggaa gaaaatccaa atgtagacgg
      961 aatctggctg gaggatactt atcagagaga atatccatat aattcgctgg cctgcgatgt
     1021 gatcggattt gtacagggag ataatgaagg tgcgta
//
LOCUS       DAOSYE010000910         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_137174_length_1056_cov_2.328671, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000910 DAOSYE010000000
VERSION     DAOSYE010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137174_length_1056_cov_2.328671"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..596)
                     /locus_tag="PLU43_12585"
     CDS             complement(42..596)
                     /locus_tag="PLU43_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOO29294.1"
                     /translation="MISREEIKNEAKQQIRGKIGMLFICMLLMVVIAGASDVIPLAVI
                     VIQPPMMLGSIYVYLDICAGGEVDINRLFAGFKRLGQSICLFLLISVYTFLWSLLFVI
                     PGIIKAISYSQSFYILAEHPEMTASEALRESMEIMEGHKWKYVVLYLSFIGWVLLGAV
                     TCGIAFIWVKPYMSATLVNYYHRL"
     gene            complement(621..>1056)
                     /locus_tag="PLU43_12590"
     CDS             complement(621..>1056)
                     /locus_tag="PLU43_12590"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOO29295.1"
                     /translation="GLGSEYKTGRADSQVISFRQDVYEFTGGVHPNNAAAGYNFSTET
                     GELLTLKDVVSDETAAKEFIADEILQQTKESIYSGMLFDDYEDEIADVFSDAVWYFSE
                     DGFHVICNEYILGPHFTGILEFVIPYDQFTLLNDEYLLRAEK"
BASE COUNT          316 a          273 c          177 g          290 t
ORIGIN      
        1 aataatctcc tctatgaaac aatattgtca gtcctgcagc ctcatagtct atgataataa
       61 ttgaccagtg ttgcagacat atatggcttg acccatataa aggcaattcc gcacgtgacc
      121 gccccaagaa gcacccagcc gataaaagac aggtacagta caacatattt ccatttatgt
      181 ccttccatga tttccatact ttcccgcagt gcttcgcttg ccgtcatttc aggatgttcg
      241 gccaaaatat aaaacgactg tgaataagaa atcgctttga taattcccgg aataacaaaa
      301 agcagcgacc ataaaaatgt atagacactg atcagcaaaa acaggcagat cgactggcca
      361 agccgtttaa atcctgcaaa cagccggttg atatccacct cgcctcctgc acagatatcc
      421 agatatacat agatacttcc aagcatcata ggcggctgaa ttacaattac ggctaatgga
      481 atcacatcgg aagcacccgc aataacgacc ataagcagca tacatataaa taacatccct
      541 atttttcctc tgatctgctg ttttgcctcg ttctttatct cttctcttga aatcattttc
      601 atttccctcc tcattttcta ttatttttcc gctctgagca gatactcgtc attcagcaat
      661 gtaaactggt cgtatggaat cacaaactcc agaatacctg taaaatgggg ccccaatata
      721 tattcattgc aaatcacatg aaatccgtct tcggaaaaat accataccgc atcggaaaaa
      781 acatcagcaa tctcatcttc gtaatcatca aaaagcattc cgctgtaaat ggactctttt
      841 gtctgctgca gaatctcatc ggcaataaat tccttcgccg ccgtttcatc ggaaacaaca
      901 tcctttaggg tcagaagctc tccggtctcc gtagaaaaat tatatccggc tgccgcatta
      961 ttcggatgta cgccgccggt aaattcgtag acatcctgtc tgaagctgat gacctggctg
     1021 tctgccctgc cggttttata ctcacttcca agacca
//
LOCUS       DAOSYE010000911         1051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_138183_length_1051_cov_3.413655, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000911 DAOSYE010000000
VERSION     DAOSYE010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138183_length_1051_cov_3.413655"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLU43_12595"
     CDS             <1..198
                     /locus_tag="PLU43_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29296.1"
                     /translation="GYMLQEGLQYLYTHTFLAISPAIFIIITVINLNVLSDNIQLYLD
                     PTRRKLPSFKKLEQMEEKRNE"
     gene            191..>1051
                     /locus_tag="PLU43_12600"
     CDS             191..>1051
                     /locus_tag="PLU43_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29297.1"
                     /translation="MNKDSVVNIKNLKVDLASINGIVHAVRDINLDIKKGEVHGIVGE
                     SGCGKSMTVKTIMRLHDEKRTRYHGEIIYNHKNILEMKKRELNAIRGRDISMIFQDPM
                     VGLDPIYSAGEQVGEMIREKKHMKKAEAKAQVLKLFEDVGIYPAEKRYAQYPFEMSGG
                     MLQRVMIALALSLEPQLLIADEPTTALDVTIQLQILELLKSLQKKNGMAIILVTHNLG
                     VIAEICDRVSVMYAGKILETSSVYDIFDNPCHPYTNALMDSSPKAGQKGKRLITIPGT
                     PPKLFGRLEGC"
BASE COUNT          346 a          184 c          261 g          260 t
ORIGIN      
        1 ggatatatgc tgcaggaagg tctgcagtat ttatatacac acacgtttct ggcgatcagc
       61 cctgcgatct ttattattat aacggtgatc aatctgaatg tgttgagcga caatattcaa
      121 ttatatctgg acccgaccag aagaaaatta ccatcattca aaaaactgga acagatggag
      181 gaaaaaagaa atgaataagg acagtgtagt caatattaaa aatctgaaag tagatcttgc
      241 ttcaatcaat ggtatcgtac acgctgtcag ggatatcaat ctcgatatta aaaaaggtga
      301 agtgcacggt attgttggtg aaagcgggtg cggcaagtcc atgaccgtaa aaacgatcat
      361 gcggctgcat gatgagaaga gaacaagata tcatggagaa attatatata accataaaaa
      421 tatacttgag atgaaaaaga gagaactaaa tgcaatcaga ggaagggata tctcaatgat
      481 tttccaggat cccatggttg gtctggaccc gatctattcc gcaggcgaac aggttggaga
      541 aatgatcagg gaaaaaaagc atatgaaaaa ggctgaagca aaagcacagg ttttaaagct
      601 ttttgaggat gtgggaattt atccggcgga aaaaagatat gcgcagtatc cgttcgaaat
      661 gagcggcggc atgctgcagc gtgttatgat cgcacttgca ctttctctgg aaccgcagct
      721 cttgattgca gatgaaccta ctacggcatt ggatgtaacg atacagctgc agattctgga
      781 attgctgaaa tcccttcaga aaaagaacgg gatggcaatt atactggtga cacataattt
      841 gggcgttatt gcagagatat gtgacagggt atcggtaatg tatgcgggta aaattcttga
      901 aacaagcagt gtatatgata tttttgacaa cccctgccat ccctatacaa atgcattgat
      961 ggacagcagt cccaaggccg ggcagaaggg gaagcgtctg attacgatcc ctggtacacc
     1021 gccgaagctt tttggcaggc tggaaggatg t
//
LOCUS       DAOSYE010000912         1049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_138797_length_1049_cov_1.738431, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000912 DAOSYE010000000
VERSION     DAOSYE010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138797_length_1049_cov_1.738431"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLU43_12605"
     CDS             <1..453
                     /locus_tag="PLU43_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29298.1"
                     /translation="GSHNPCAIPAIYRKFMKGNDIVIGSRYVKGGMTHDAKSSVLMSH
                     ILNGVMRVCIGVKAKDISTAYRLYDANLLKAVVLKEENYDVLQEVILKMRIEKRRQKK
                     GALRIGEIPIEFEKRMYGESKRRLLKFIFSYIGTVFKLLHVRIQAGKG"
     gene            455..>1049
                     /locus_tag="PLU43_12610"
     CDS             455..>1049
                     /locus_tag="PLU43_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29299.1"
                     /translation="MKKNDTVVLKQSQKQIYFILAAVSLTYFLYMLCFCNLRSLWVDD
                     LAQIEIAVQPSIIAMLKETIFLDNNPPLFHLISYFWIRLAPYGTGWLKLPGMVFASIG
                     LYVCGLTAYRLFGKPAALFCVAMGFLQKGLVSLAAYTFRPYGLYFCLLSFMLFFYVKS
                     RMQFTWKRNLLFMISLILAVYTHYFSILFCIVFFLFDL"
BASE COUNT          270 a          189 c          252 g          338 t
ORIGIN      
        1 ggttcgcata atccatgtgc aattcctgct atttacagaa agtttatgaa agggaatgat
       61 attgtgatcg gttcccgtta tgtgaaaggc ggaatgacac atgatgcaaa aagctctgtt
      121 ctgatgtctc atattctgaa cggggtcatg cgcgtatgta tcggagtcaa agcgaaggat
      181 atttccacgg cgtaccggtt atatgatgca aacctgctga aagcggtcgt tctgaaagaa
      241 gagaattacg atgttctgca ggaagtgatc ttaaagatga gaatagaaaa gcgcagacag
      301 aaaaaaggag cgctgcggat cggggaaatt ccgatcgaat ttgaaaagcg gatgtatgga
      361 gagtcaaagc gccgtctgct gaagttcatt ttcagttata tcggtaccgt atttaaactg
      421 ttgcacgttc ggatacaggc aggaaagggt taagatgaaa aagaatgata ccgtagtttt
      481 aaaacagtct caaaaacaga tatatttcat tttggctgcc gtttctttga catattttct
      541 ttatatgctc tgcttttgca atctgcgatc tctctgggtg gacgatcttg cgcagatcga
      601 aatagcagtg cagccgtcga ttattgctat gctgaaggaa acgattttcc tggataataa
      661 tccgcctctt tttcatctga tctcctattt ttggatccgc cttgcaccat atgggacagg
      721 ctggctgaag ctgcccggta tggtatttgc aagtattgga ctgtatgtat gcggattgac
      781 cgcgtatcgg ctttttggaa aaccggccgc attgttttgc gtggccatgg gatttctgca
      841 aaaaggtctt gtatcactgg ctgcttatac gttccgtccg tatggtctgt atttttgctt
      901 actttctttt atgctgtttt tctatgtaaa aagcaggatg cagtttacat ggaaaagaaa
      961 cctgctgttc atgataagcc tgatacttgc ggtgtatacc cattattttt cgatattgtt
     1021 ctgtatcgtt ttttttctgt ttgatcttt
//
LOCUS       DAOSYE010000913         1042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_140194_length_1042_cov_2.792300, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000913 DAOSYE010000000
VERSION     DAOSYE010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140194_length_1042_cov_2.792300"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1042)
                     /locus_tag="PLU43_12615"
     CDS             complement(<1..>1042)
                     /locus_tag="PLU43_12615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO29300.1"
                     /translation="SNEEVKKQRNRAAIKNKQYFLFPHRLKNGEIKFVDLYSSPIEIN
                     DRQYLFSIITDVTKREQNEIELYKEKELQRITMDSIADCVVTTDEQGRITYLNNAAAI
                     AAEWKQAEVIGKYFNEIFPMTNEYTQDTVPNIVEAVLKTSHKQELANHTMLHTKSGNK
                     IPIEDSAAPILDKNGKRYGAVVIFRDVTAAKEKKKKIKYLSYHDALTGLYNRNFYYDY
                     IDHSNNTGIYPLGYIIGDVNGLKMTNDVFGHTMGDNLLKTVSDILQKTADTNDRVIRW
                     GGDEFIIVVPHADRKHLESLIVKIKSDLKSITITDGIEASVSFGMSIKQHKWISTDDI
                     LKEAEEMMYQTKL"
BASE COUNT          259 a          208 c          199 g          376 t
ORIGIN      
        1 cagcttggtc tgatacatca tttcttccgc ttctttgagg atgtcgtcgg tagagatcca
       61 tttgtgctgt tttatggaca tgccaaaaga aacactggct tcaataccgt cggttattgt
      121 tatggatttc aaatcggatt ttattttgac gatcaaagac tccagatgtt ttctgtccgc
      181 atgcggaact acaataataa attcatcgcc gccccagcga ataactctgt cattggtatc
      241 ggcagttttt tgaaggatat cactgactgt tttaagtaaa ttgtctccca ttgtatgacc
      301 gaacacatcg tttgtcattt ttaaaccgtt tacgtctcct atgatatagc ctaaaggata
      361 aatgcctgta ttattggagt gatcaatata atcatagtaa aaattcctgt tgtataagcc
      421 tgtcagtgca tcatggtagc tgagatattt gattttcttc tttttttctt tggcagcggt
      481 cacatctctg aaaatgacaa cggcaccata gcgttttccg tttttgtcta aaataggtgc
      541 ggcactgtct tcaatcggaa ttttatttcc tgattttgtg tggagcatcg tatgatttgc
      601 gagttcctgc ttatgacttg ttttcagaac agcctctacg atgtttggaa cagtatcctg
      661 tgtgtattca tttgtcatag gaaaaatttc attaaaatat tttccgatca cctctgcctg
      721 tttccattcc gcagcaatgg cagccgcgtt attcaggtaa gtgatccggc cctgctcatc
      781 tgtcgtgact acgcagtctg caatagaatc cattgtaatg cgctgcagtt ccttttcttt
      841 atacagttcg atttcattct gctcgcgttt tgtaacatcc gtaatgatgg aaaataaata
      901 ctgtctgtcg ttgatctcaa taggactgga gtataagtca acaaatttga tttcaccatt
      961 ttttaaccga tggggaaata aaaaatactg cttatttttt attgcggccc ggtttcgctg
     1021 cttttttact tcttcattac ta
//
LOCUS       DAOSYE010000914         1040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_140650_length_1040_cov_2.623350, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000914 DAOSYE010000000
VERSION     DAOSYE010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140650_length_1040_cov_2.623350"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1040
                     /locus_tag="PLU43_12620"
     CDS             <1..>1040
                     /locus_tag="PLU43_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29301.1"
                     /translation="LKEEYSLNKMISDIRDIIALRAEESYLTFTIRWDPMLPDRLLGD
                     EGRVRQILLNILNNAVKYTDIGSVNLSVDFVLLKDNKINLSFAVTDTGIGIREEDQKQ
                     LFRMFSQVDAKKNREKEGTGLGLAISMRLAELMNGTITVDSTYGKGSKFTVTIEQEAV
                     DFTYAVYNEHPDKYHAYIFMREDEKWGFGRMLNNLQIQIEEEGAVLHAEDSDSTIIFF
                     DMKTYMDVYEATWRNKFSSARMIGLTEIKKAAEETEGIQVLRKPALPIEIMRLLAGDQ
                     ESDRQEKAGDLYGFEAPEAKILAVDDNRVNLKVITEILKRYEVQIFPVESAQEAIDQI
                     KSGDRYDLILMD"
BASE COUNT          332 a          163 c          275 g          270 t
ORIGIN      
        1 tctgaaagag gaatattccc tgaataagat gatttccgat atccgtgaca tcattgcgct
       61 tcgtgcggag gaaagctatc ttacatttac aataagatgg gatccgatgc ttccggaccg
      121 gctgcttggt gacgaaggac gtgtcaggca gattttgctg aatattttga ataatgcggt
      181 taaatatacg gatatcggct cggtaaattt aagtgttgat tttgtattat tgaaagacaa
      241 caaaatcaat ctttcttttg cggtgacgga tacagggatc ggaatcaggg aagaggatca
      301 aaaacagctg ttcagaatgt tttcgcaggt agatgcaaag aaaaatcgtg aaaaagaagg
      361 gacaggactc ggccttgcaa tttccatgag acttgcagaa cttatgaatg gcaccattac
      421 ggtagacagt acgtatggaa aaggaagcaa atttacggtt acgatcgaac aggaagcggt
      481 agattttact tatgcggttt ataatgagca tccggataaa tatcatgcgt atatttttat
      541 gcgtgaggac gaaaaatggg ggtttggccg aatgcttaat aatctgcaga ttcagatcga
      601 ggaagaggga gctgttttgc atgcggaaga ttcggacagt acgattattt tctttgatat
      661 gaaaacgtat atggatgttt atgaagcaac atggagaaac aaattcagca gtgcaagaat
      721 gatcggtctg acagaaatca aaaaggctgc tgaagaaaca gaaggtatac aggtacttag
      781 aaagccggcg cttccgatcg aaatcatgcg gctgcttgca ggtgatcagg aatcggacag
      841 acaggagaaa gccggtgatc tgtatggttt tgaagcaccg gaagcaaaaa tacttgctgt
      901 tgacgataac cgtgtgaatc tgaaagttat tacggaaata ctgaagcggt atgaagtaca
      961 gatttttccg gtggagagtg cacaggaggc aattgatcag ataaaatcag gcgaccggta
     1021 tgatctgatc ctgatggatt
//
LOCUS       DAOSYE010000915         1036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_141510_length_1036_cov_3.367992, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000915 DAOSYE010000000
VERSION     DAOSYE010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141510_length_1036_cov_3.367992"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLU43_12625"
     CDS             complement(<1..219)
                     /locus_tag="PLU43_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29302.1"
                     /translation="MKTKSEKKFQIFAHIVMIILSLTAVIPFLLLVISSFTDNDTLIS
                     DGYSFTPAKWSIYAYQYIFKTGNSVMHAY"
     gene            complement(243..>1036)
                     /locus_tag="PLU43_12630"
     CDS             complement(243..>1036)
                     /locus_tag="PLU43_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO29303.1"
                     /translation="KYNARKGIFGSEFVGLKNFKYLFATKDAFIITRNTICYNAVFIV
                     LNTVFAIFVAILLSEMTSKLKKTYQCLILLPYMISMVIVSYLVFGFLSTENGFLNNTV
                     LKSLGMEPVAWYMEKKYWPFILTFTNCWKVLGYNCVIYMATILGIDRSIFESAAIDGA
                     SKWQQIKSITIPLLKPTVIMLTLLAIGRIFYSDFGLFYQVPQNQGALFSVTNTIDTYV
                     YRGLLELGDISMSSAAGLYQSVVGFILVLGANLIIRKIEPDSAMF"
BASE COUNT          341 a          228 c          177 g          290 t
ORIGIN      
        1 gtatgcatgc atcactgaat ttcctgtctt aaatatatat tgatatgcgt agatactcca
       61 ttttgcagga gtaaacgaat agccgtccga gatcagcgtg tcattatccg taaaagaaga
      121 aataaccagc agcagaaacg gtatgaccgc cgttaaagat aaaatgatca tgactatatg
      181 cgcaaatatc tgaaatttct tttcactttt tgttttcata ctcaacccat gccctttatt
      241 atttaaaaca tcgcactgtc cggctcgatt ttccgaatga tcagattcgc gccgaggaca
      301 agaataaacc ctacgaccga ctgataaaga ccggcagccg acgacataga aatatctcca
      361 agttccagca atcctctgta cacatacgta tcaatcgtgt ttgttacgga gaaaagcgct
      421 ccctgattct gcggcacctg atagaacaga ccgaaatccg aataaaaaat acgcccgatc
      481 gccagcagtg taagcataat gactgttggc ttcagcagcg ggatcgtaat gcttttgatc
      541 tgctgccatt ttgacgcacc atcgattgca gccgattcaa aaatacttct gtcaatacca
      601 agtatcgtcg ccatgtaaat aacgcaatta tacccgagta ctttccagca atttgtgaat
      661 gtcagaataa agggccagta cttcttctcc atataccagg cgaccggttc catccccaat
      721 gattttaaaa cagtattatt caaaaatccg ttttccgtag ataaaaatcc gaaaaccaga
      781 taactgacaa taaccatgga aatcatgtaa ggcaaaagga tcagacactg atatgtcttt
      841 tttaattttg aagtcatttc ggataacaga atcgccacaa atattgcaaa gaccgtatta
      901 agaacaataa ataccgcatt gtagcagatc gtatttcttg taataataaa tgcatccttt
      961 gttgcaaata aatatttgaa atttttcagt cccacaaact cagaaccgaa aatacctttt
     1021 ctggcattat attttt
//
LOCUS       DAOSYE010000916         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_141748_length_1035_cov_4.170408, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000916 DAOSYE010000000
VERSION     DAOSYE010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141748_length_1035_cov_4.170408"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLU43_12635"
     CDS             <1..884
                     /locus_tag="PLU43_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29304.1"
                     /translation="TTQKASEYLKNAYNSTVVNGISYAVPFTSDPYVIFYNEDYLVRY
                     GAKMPERMEDFYELCNETSTLGTYNLGIAVKNKEDVTSCFLQMIYSSGGTIRNLDTPD
                     CMRLYGILGDMRDEDLISQDVINWNQKDLMEAFSKGLVNMAIAKLSSMSVLENKEMKF
                     NYKIAEIPYIQKQAYLLQGENIGITTAADYQEALKLTEYLTSEEVVKAYCEKTYCLSV
                     RSDITVNPAKNRGLSDAFVEKERNQNILKSSYTSWFIISDAIAENLADFFGDKTVTPD
                     QIGESMQEDVRSAIMER"
BASE COUNT          357 a          157 c          247 g          274 t
ORIGIN      
        1 tgacgacgca gaaagcttcc gaatatttga aaaatgcata taacagtacg gtcgtgaatg
       61 gaatttccta tgcggttcca tttacaagcg atccctacgt catattttat aatgaagact
      121 atcttgtcag atatggggca aagatgccgg aaagaatgga agatttttat gagctgtgta
      181 atgagacaag tacactggga acgtataatc tggggattgc ggtcaaaaat aaagaggatg
      241 tgacctcctg ttttcttcaa atgatctatt cctccggagg aacgatcaga aatctggaca
      301 caccggattg catgcggctt tatggaatac tcggggatat gcgggatgaa gatctgattt
      361 cgcaggacgt gatcaactgg aatcagaaag acctgatgga agcgttttcc aaagggcttg
      421 ttaatatggc gatcgcaaaa ctgagttcca tgtctgtttt agaaaataag gagatgaaat
      481 ttaattataa aattgcggag attccctata ttcaaaagca ggcctatctt ttgcagggag
      541 aaaatattgg gatcacaacg gcggctgatt atcaggaagc gctgaaattg acagaatatc
      601 tgacttcgga agaagttgtg aaagcatatt gtgaaaaaac atattgttta tcggtaagat
      661 cggacattac ggtaaatcct gcaaagaaca gggggctttc cgatgcattc gtggaaaaag
      721 agagaaatca gaatatcctg aaaagttcct atacatcgtg gtttataata tcagatgcaa
      781 tagcggaaaa tctggcggac ttttttggag ataaaacagt tacaccggat cagatcggag
      841 aaagcatgca ggaggatgtc agaagtgcga tcatggagag atgacctgaa aagatttgga
      901 agagattaaa attgtacccc ttttataaaa ttttggaata tggaatcaaa aacagctgtg
      961 ttatgatgtg cctatcaaag aaaagatagt gtcaaatgat ttatgaaaaa actgagtcaa
     1021 aaaaataaga cttat
//
LOCUS       DAOSYE010000917         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_141846_length_1035_cov_2.393878, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000917 DAOSYE010000000
VERSION     DAOSYE010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141846_length_1035_cov_2.393878"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLU43_12640"
     CDS             complement(<1..495)
                     /locus_tag="PLU43_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOO29305.1"
                     /translation="MPYIGPNASLPSADDSFHEVKSRETALFSFETYRNINLTGTLFI
                     TSHWYHEIKILCFQKGEADVYISGQTLHVHPGDVVFINQEELHQITSDDPDILYHACL
                     FPLEFLYFSRPDYTQNHYLTPLSNKELLLPHYMERGDSFTDAIYTELIDIIATFDHKE
                     AAYQL"
     gene            650..>1035
                     /locus_tag="PLU43_12645"
     CDS             650..>1035
                     /locus_tag="PLU43_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO29306.1"
                     /translation="MDKKMKVGIIGCGGIARGKHLPAIRKQGNIEVAAFCDLIEERAE
                     EFRVKFGWPDARVFTDYRELLKEDLDAVYVLTPNREHSFITIDALRAGKNVMCEKPMA
                     KTQEEAQAMLDAAKETGKLLTIGYQNR"
BASE COUNT          331 a          184 c          259 g          261 t
ORIGIN      
        1 gagctggtag gccgcctcct tgtggtcgaa tgttgcgata atatcgatca gttccgtgta
       61 gatcgcatca gtaaagctgt cgccgcgttc catgtaatgc ggaagcagaa gctctttgtt
      121 tgacagtggt gtcaggtagt gattctgtgt gtagtccggt ctggaaaaat ataagaattc
      181 aagtggaaag agacaggcgt gatataagat atcaggatcg tccgaagtga tctgatggag
      241 ttcttcctga ttgatgaata caacatcacc cggatgaaca tgaagcgtct gaccgctgat
      301 atagacatct gcttctccct tttggaaaca gaggatcttg atttcatgat accagtgcga
      361 tgtgataaaa agcgttcccg taagattgat attccggtaa gtttcaaaag aaaacagagc
      421 agtttcccgt gattttactt catggaagga atcatctgca gatggaaggg aagcgtttgg
      481 tccgatatat ggcataacaa aacagcacct tctttcaaaa tatagaaatt gtgttattaa
      541 aacgcgatat aatacaagaa atattaaaaa tatatagata taatacaatc ataacaagca
      601 gatccatatt tgacaagtaa taataaaact agaactggag gacctttata tggacaaaaa
      661 aatgaaagtt ggaattattg gctgcggagg aattgcaagg ggaaagcatc tgcctgcgat
      721 tcgaaaacag ggtaatatcg aagtggccgc attttgtgat ctgatcgagg agcgtgccga
      781 agagttccgt gtgaaatttg gctggccgga tgccagagtc tttacggatt acagggagct
      841 gctcaaagag gacctggatg ctgtctatgt gctgacacca aatcgagaac acagctttat
      901 tacgatcgat gccctgcggg ccggcaaaaa tgtgatgtgt gaaaagccaa tggcaaagac
      961 acaggaagaa gcacaggcga tgttagatgc agccaaagaa acaggaaaac ttcttacgat
     1021 cggttatcag aaccg
//
LOCUS       DAOSYE010000918         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_142078_length_1034_cov_2.343207, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000918 DAOSYE010000000
VERSION     DAOSYE010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142078_length_1034_cov_2.343207"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU43_12650"
     CDS             <1..886
                     /locus_tag="PLU43_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO29307.1"
                     /translation="YYQPEEADTGVYLNKIKAAVKNGAKLIVCPGYLPEEAVYKAQDK
                     YPKVHFILLDGTPHNADYSDTEINDNVMPILFEEDQAGFLAGYAAVRDGYTHLGFIGG
                     VAEAPVIRYGYGYVQGADYAAIEMGTSVDIKYCYSGTFIEDPDVQSMASSWYQDGTEV
                     IFACGAAMNRSVMSAAEGSLTDAKVIGVDIDQSAESPTVITSAMKMLSNAVYGGIKDF
                     YDDSFKGGEITTLTAKDGGVGLPMETSVFRSFTSADYDEIFTLLVEDVIVPYASTEVG
                     TTDDLTLVNTTVNYISME"
BASE COUNT          291 a          184 c          277 g          282 t
ORIGIN      
        1 atattaccag cctgaagaag cagataccgg cgtgtatttg aataagatca aggcagcggt
       61 caaaaacggt gcaaaactga tcgtttgtcc cggatatctt cctgaagaag cagtatataa
      121 ggcacaggac aaatatccta aggtccattt tattttattg gacggaacgc ctcataatgc
      181 agactattcg gatactgaaa tcaatgataa tgttatgccg atcttatttg aagaggatca
      241 ggcgggcttt ttggcaggat atgccgctgt gcgggacggc tatacacatc ttgggtttat
      301 cggcggggtt gcggaggcac cggttatcag atatggatac ggttatgtac agggcgccga
      361 ttatgcggca atcgaaatgg gcacgtctgt tgatattaag tattgttatt ccggaacatt
      421 tattgaggat cctgatgtac aatccatggc ttcatcatgg tatcaggatg gaacggaagt
      481 gatttttgca tgcggagcgg cgatgaaccg ttctgtcatg tcggcagcag aaggatcgct
      541 gacggatgca aaggtgattg gggttgatat cgatcagagt gctgaatcgc cgactgtgat
      601 cacgtctgcg atgaagatgc tttccaatgc ggtatatggt ggtattaagg atttctatga
      661 tgattctttt aaaggcggag aaataacaac cttgacagcc aaagacggcg gagtcggact
      721 tccaatggaa acgtcggtat tcaggagctt tacgagcgcc gattatgatg agatctttac
      781 actgcttgtg gaagatgtga tcgttccgta tgccagtacg gaggtgggaa cgacagatga
      841 tctgacactt gtaaatacaa cggtgaatta tatttcaatg gaataaccgt atttattaat
      901 acgaaaatgg agatggccat ggaaaacata caattgatca atgttgcatt tgaagcaagg
      961 aaagcagcat atactcctta ttccggatat cgtgtcggtg cggctcttct gacagctaag
     1021 ggagagatta tttc
//
LOCUS       DAOSYE010000919         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_142235_length_1033_cov_3.489775, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000919 DAOSYE010000000
VERSION     DAOSYE010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142235_length_1033_cov_3.489775"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>1033)
                     /locus_tag="PLU43_12655"
     CDS             complement(164..>1033)
                     /locus_tag="PLU43_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HOO29308.1"
                     /translation="RTALMLEPRGHKDMFGALITAPVHEEADLGVIFMDSGGYLNMCG
                     HGSIGTASMAVETGLVEVREPYTTVILDTPSGMIHTCVTVKEGKAEKVSILNVPSFLY
                     KEEETLEIDGFGKITFDISFGGSFFALVDARQIGLQLVPGCIAEITELGMKLREKINQ
                     EIPVIHPYLDITNVDLVEFYGDTQNPKADLRNCVIFGSAQSDRSPCGTGTSAKMAALY
                     AKGKLKLKEPFVYESIMGSLFTGRIVSETTVMDRRCIIPEITGSAYITGLNNWILDEK
                     DPLEAGFLPGKSR"
BASE COUNT          256 a          290 c          209 g          278 t
ORIGIN      
        1 gaaaaatcag ctgttcttca gaagtcactg ctgcacagct gttttatggc atgatccatg
       61 gatgtctgac atggaatgca cttcttacag acggagcttc tctgcaggaa gccgacggtc
      121 tggccgaaga aatcatcatg acctttttga cccgctacga cgcctagcgg cttttacccg
      181 gcagaaagcc tgcctccagc ggatcctttt catccagtat ccaattgttc agtccggtaa
      241 tatatgcact tccggtgatc tccggtataa tacaccgtct gtccatgaca gttgtttccg
      301 aaacgatccg tccggtaaat aaagaaccca taatactttc atagacaaat ggttccttca
      361 gcttcagctt tccttttgca tataatgctg ccattttagc gctcgtccct gtcccgcacg
      421 gagaccggtc tgactgtgcg ctgccaaaga tcacgcagtt tcgcagatca gctttcggat
      481 tctgcgtatc cccgtaaaat tccacaagat ccacatttgt gatatccaga tacggatgaa
      541 ttaccggtat ttcctgattg attttctccc taagcttcat accgagctct gtgatttccg
      601 caatacaccc cggaacaagc tgcagcccga tctgcctggc atcgaccagc gcaaaaaaag
      661 aaccgccaaa ggaaatatca aacgttattt tcccaaatcc gtcaatttcc aatgtttctt
      721 cctccttgta caaaaaagag ggaacattta aaatgctgac cttttccgct tttccttctt
      781 ttaccgtaac acaggtatga atcataccgg atggcgtatc caaaatgact gttgtatatg
      841 gttcccgcac ttctacaagt cctgtctcga ccgccatgga tgccgtaccg atgctgccat
      901 gtccgcacat gttcagatac ccgccgctgt ccataaagat cacgccaaga tctgcctctt
      961 catgcaccgg agccgtgatc agcgcaccga acatgtcttt atggccgcgg ggttccagca
     1021 tcagcgcagt acg
//
LOCUS       DAOSYE010000920         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_142372_length_1033_cov_1.948875, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000920 DAOSYE010000000
VERSION     DAOSYE010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142372_length_1033_cov_1.948875"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLU43_12660"
     CDS             <1..333
                     /locus_tag="PLU43_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29309.1"
                     /translation="WLAYVDVVLLAFTFITNGWSDRIAIIASLLNFILFFLGTRNYRR
                     VSPKEMHRRHSYKQEVHHADGITKHKCAVCGRTELDGDDLEFRFCSKCNGNYEYCQDH
                     LFTHEHIR"
     gene            362..>1033
                     /locus_tag="PLU43_12665"
     CDS             362..>1033
                     /locus_tag="PLU43_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOO29310.1"
                     /translation="MKNEQAFKKLLIRIRVQAKKQDGFILEDTVRQMLPDMELSEEEF
                     QLVIKYLGEAQITVVSGTEEDLDSIRKAAEAETEEKERPGSAAYDLYIKELGELGEIG
                     EEERLKKTEQALADRATVHETIPLLYLREVVDVAKLYGGQGVSAEDLIGEGNISLLTG
                     AKMLECCDTAKEAEEFLVKMIMDAMESLLMEKSSEDELDLKVLERVNDLNEKAKELAE
                     TVGREV"
BASE COUNT          313 a          176 c          292 g          252 t
ORIGIN      
        1 tggcttgcct atgtcgatgt ggttttgctg gcctttacct ttattacaaa cggctggtcc
       61 gacaggatcg cgatcatagc gtcacttttg aattttattt tgtttttcct tggcacgcgc
      121 aattacagac gtgtttcgcc gaaagaaatg cacagacgtc attcctataa gcaggaagta
      181 caccatgcgg atgggatcac aaagcacaaa tgtgcggtat gcggacggac agaactggat
      241 ggggatgatt tggaattccg cttctgttca aaatgcaacg gcaattatga atattgtcag
      301 gatcatctgt ttacacatga acatattcgt tagaaaagcg ggcggttagg gagaaaataa
      361 aatgaaaaat gaacaggcat ttaaaaagct tctgatacga atacgggtgc aggcaaaaaa
      421 gcaggacggc tttattttag aagatacggt cagacagatg ctgccggata tggaacttag
      481 tgaggaagag tttcagcttg tgatcaagta tctgggtgaa gcacagatca cggttgtttc
      541 agggacagag gaggatctgg attccatacg taaggctgcc gaagctgaaa cagaggaaaa
      601 agaacgaccg ggatccgcag catatgatct gtacataaag gaactcgggg aacttggtga
      661 gatcggtgaa gaagagcggc ttaaaaagac ggagcaggcg cttgcagaca gggcaactgt
      721 ccatgaaacg attccgctgc tgtatctgcg tgaggttgtg gatgtagcca agctttatgg
      781 agggcagggc gtttcagccg aggatctgat cggagaagga aatatttctc ttttaacagg
      841 tgcaaaaatg ctcgaatgtt gtgatacggc aaaagaagct gaggaatttc ttgtgaagat
      901 gattatggat gcgatggaat cgctgctcat ggaaaaatct tcggaggatg aactggactt
      961 aaaagtactt gaacgtgtta atgacctgaa tgaaaaggca aaagagcttg cggaaacagt
     1021 agggcgtgaa gtg
//
LOCUS       DAOSYE010000921         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_143905_length_1026_cov_3.913491, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000921 DAOSYE010000000
VERSION     DAOSYE010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143905_length_1026_cov_3.913491"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLU43_12670"
     CDS             complement(<1..597)
                     /locus_tag="PLU43_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HOO29311.1"
                     /translation="MKEAVAMLKKGEGRLLKAGGMWIFDNEIASVTGDFADGDLVAVH
                     DFDGYPLGKGFINRASKIRIRMLTRDAGRQINDDFLYERIRAAWEYRKMTVDTASCRL
                     IFGEADFLPGIVIDKFSDVLVVESLALGIDKLKIRLIGILKDVLKEDGIIIRGVYERS
                     DAKVRENEGMERTKGFLSEAFDTDVEIVENGIKYIVDVK"
     gene            complement(625..>1026)
                     /locus_tag="PLU43_12675"
     CDS             complement(625..>1026)
                     /locus_tag="PLU43_12675"
                     /inference="COORDINATES: protein motif:HMM:NF037720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29312.1"
                     /translation="QKELCEVLKDKADLGVRLRYTYQTKDRQGLLVLANETIPAVLKS
                     LDRFFAAFERQWMRENKSFGFEIQCLRIGGLSKRLEFIRRVLLSYLAGEQEMIEELEQ
                     MRKPFHYLNRTDIDTLRYHMWSDMVSAGVIG"
BASE COUNT          258 a          310 c          177 g          281 t
ORIGIN      
        1 ctttacatcc acgatgtatt tgatcccgtt ttcaacgatt tccacatccg tatcaaatgc
       61 ttccgaaaga aagccctttg tacgttccat tccttcattt tcacgcacct ttgcatcact
      121 gcgttcatat acacccctga taatgatccc gtcctccttc aggacatctt tcaggatccc
      181 gatcagcctg atcttcagtt tgtcgattcc aagcgcaagc gattccacaa caagcacatc
      241 ggaaaacttg tcgattacga ttccaggcaa aaaatccgct tctccgaaaa tcagccggca
      301 gcttgccgtg tccactgtca ttttccgata ttcccatgcc gcacggatcc tctcatataa
      361 aaaatcatca ttaatctgcc ggccggcatc ccgtgtcagc atacggatcc ggatctttga
      421 cgctctgttg ataaatcctt ttccaagcgg atacccgtca aaatcatgta cggcaaccag
      481 atcaccgtct gcaaaatcgc cggtaaccga agcgatttca ttatcaaata tccacatacc
      541 gcccgccttc agcagacggc cttcaccctt ttttaacatt gcgactgctt ccttcatctt
      601 cataccttcc ttttctgtca aagcttatcc gatcacacct gcggagacca tatcactcca
      661 catatgatag cgaagtgtgt caatatccgt ccggttcaaa taatggaacg gctttctcat
      721 ctgttccagc tcctcgatca tctcctgctc tccggcaaga taagagagca gtacgcgtct
      781 aataaactcc agacgctttg acaggccgcc gatgcgcaga cactgtattt caaatccaaa
      841 ggatttgttt tcccgcatcc actgtctctc aaacgctgca aaaaatctgt caagactttt
      901 cagtacggca ggaatcgtct cattggcaag caccagaagc ccctgtctgt ccttagtctg
      961 ataagtgtac cgtaagcgca cgccaagatc cgccttgtcc ttcagcacct cacaaagttc
     1021 cttttg
//
LOCUS       DAOSYE010000922         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_143942_length_1026_cov_2.921730, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000922 DAOSYE010000000
VERSION     DAOSYE010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143942_length_1026_cov_2.921730"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLU43_12680"
     CDS             <1..662
                     /locus_tag="PLU43_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO29313.1"
                     /translation="AGGLLFLCNPNNPIGNVIEKDLMLRIVKKAEERNLYVVIDECFL
                     PFLPEYASVSFRSAYQNHKNACVINAFTKLYAMPGLRLGFVLSADRELLTKLKEQLPA
                     WNVSAVAQAAGTAALLDPAYLEKTYQWITAERSYVGENLLLCKPRIKKVYCPAANFVF
                     FEADDMLKDHLLQRQIAVRSCGNFRGLKCGDYRAAIRSHAENKYLIEAIREEAHKWHE
                     I"
     gene            647..>1026
                     /locus_tag="PLU43_12685"
     CDS             647..>1026
                     /locus_tag="PLU43_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HOO29314.1"
                     /translation="MARNLMIQGTMSNAGKSIITAGLLRVLHQDGYACAPFKSQNMAL
                     NSFITREGLEMGRAQVMQAEAAGTEPDIRMNPLLLKPNSDMGSQLIINGEVYGNYEAA
                     QFFAMKKDLLPIVLQAYGELAREY"
BASE COUNT          294 a          203 c          277 g          252 t
ORIGIN      
        1 aagcaggagg tctgctgttt ttatgcaatc cgaataatcc cattggaaat gtgatcgaaa
       61 aagatctgat gctgcggatc gtaaaaaaag cagaggaaag aaatctgtat gtggtgatcg
      121 atgaatgttt cctgccgttt ttaccggaat atgcgtccgt gagctttcga tccgcgtatc
      181 aaaatcataa aaatgcatgt gttatcaatg cgtttacgaa gctttatgcg atgccgggtc
      241 tgcggcttgg atttgtgctg agtgcagaca gggaactgct tactaaactg aaggaacagc
      301 tcccggcatg gaatgtttcg gcagttgcac aggcggccgg tacggcggca cttttggatc
      361 cagcctatct ggaaaaaacg taccaatgga tcacagccga gcgcagctat gtcggtgaaa
      421 atcttttgct gtgcaaacca aggatcaaaa aagtatattg ccctgccgcc aatttcgttt
      481 tttttgaagc ggacgacatg ctgaaagacc accttttgca gcgtcagatc gcggtcagaa
      541 gctgcggaaa ttttcgggga ctgaaatgcg gtgattacag ggcggcgatc cgttcgcatg
      601 cggaaaataa atatctgatc gaagcgatac gggaggaggc acacaaatgg cacgaaatct
      661 gatgattcag ggaaccatgt caaacgctgg aaagagtatt atcacagcag gtcttttacg
      721 cgtactgcat caggacggtt atgcatgcgc gccgttcaag tcacagaata tggcattgaa
      781 ttcttttatt acacgggaag gccttgaaat gggacgtgca caggtcatgc aggcagaagc
      841 agcaggaaca gagccggata tcaggatgaa tccgctcctg ttgaaaccga acagtgatat
      901 gggttcacag ctcattatta acggagaggt atatggaaat tatgaggcag cccagttttt
      961 tgcaatgaaa aaagatcttt tgccgatagt tctgcaggcg tatggagaac ttgcaaggga
     1021 atatga
//
LOCUS       DAOSYE010000923         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_143989_length_1026_cov_2.354274, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000923 DAOSYE010000000
VERSION     DAOSYE010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143989_length_1026_cov_2.354274"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLU43_12690"
     CDS             complement(<1..298)
                     /locus_tag="PLU43_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HOO29315.1"
                     /translation="MIKWWKHFKTITEHRRMVRKGCFSVGLYWQGLTHDLSKYSPSEF
                     LVGARYYQGYRSPNNAEREVIGYSSAWLHHKGRNKHHYEYWIDYSATGTKGMVPA"
     gene            complement(315..>1026)
                     /locus_tag="PLU43_12695"
     CDS             complement(315..>1026)
                     /locus_tag="PLU43_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29316.1"
                     /translation="SDADEAVTDKLGELSEDYAKTLTGDIGDYAHIGENTTYAAMESE
                     FSVSLLQYEITTKQSNTLYCEFYVPLVANHLYYANDDYDDRGTVTEWIVLDVMGYEAG
                     NDELYDEFKDAFTVFMENTSVNRKFYYSNAMYGEEILKAVEEGETPDKLDVQKLKEYK
                     NSYQESADIGDLNESILEFGETMSADERIAEADSYSFCISKDILAAYYNEDTKKVFFS
                     PDETEYPGSGYIELEMQE"
BASE COUNT          238 a          278 c          183 g          327 t
ORIGIN      
        1 gtgccggcac catgcctttc gttccggtcg cactgtaatc gatccaatac tcataatgat
       61 gcttattgcg tcctttatgg tgcagccagg cactcgaata gccgatgacc tctctctccg
      121 cattattcgg gctgcgatat ccctggtaat atctggctcc gacaagaaac tcagacggcg
      181 aatacttgct tagatcatgt gtcaggccct gccagtacaa tcctacggaa aagcatcctt
      241 ttctgaccat tctcctgtgc tccgtaatcg tcttgaaatg tttccaccac tttatcatgt
      301 tcgatctcct tttgttattc ctgcatttcc agctctatat agccgcttcc cggatattcc
      361 gtttcatccg gtgaaaaaaa tacttttttc gtatcctcgt tataataggc tgccaatatg
      421 tcctttgaga tacaaaaaga atagctgtct gcttccgcga tccgctcatc tgcggacatc
      481 gtttcaccaa actccagtat ggattcattc aggtctccga tatccgcgga ttcctgatag
      541 ctgttcttat actctttcag cttctgtaca tccagcttgt ccggcgtttc gccttcctca
      601 acagctttaa ggatctcctc cccgtacatg gcattgctgt aataaaattt tctgttaacg
      661 cttgtatttt ccatgaaaac agtaaaggca tctttaaact cgtcatacag ctcatcattt
      721 ccggcctcat agcccatcac atccaaaacg atccattctg tcacagttcc cctgtcatcg
      781 taatcatcat ttgcatagta taaatgattg gcgaccagcg gcacataaaa ttcacaatac
      841 agtgtattgc tctgttttgt agtaatttcg tattgcagca gactgaccga gaactcggat
      901 tccattgccg catacgttgt attttccccg atatgggcat agtctccgat atctcctgtc
      961 agcgttttcg cataatcttc agaaagctct cccagtttat ctgtgactgc ttcatcagcg
     1021 tccgat
//
LOCUS       DAOSYE010000924         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_144603_length_1023_cov_4.389463, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000924 DAOSYE010000000
VERSION     DAOSYE010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144603_length_1023_cov_4.389463"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLU43_12700"
     CDS             <1..204
                     /locus_tag="PLU43_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29317.1"
                     /translation="PAIFLHLVYNAVPFVIQVLPEAVLLNTVFWLLTPFLLLLIGLFL
                     FRMFGFSFSLRKVSADVSDAVRQ"
     gene            376..1014
                     /locus_tag="PLU43_12705"
     CDS             376..1014
                     /locus_tag="PLU43_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOO29318.1"
                     /translation="MKEYRLYLFDFDYTLVNSEKGIVGCFEHVFWLNGITGIEKDDIR
                     RTIGMPLAEEFRVLTGVEDKHMLERFRREFSEMADEIMTDRTELFPETLPMLERLKKR
                     NARIGIVSTKQRPRIREAIDKFYLQELIDLVIGVADVTQTKPNPQGVLRAIDFFKIDK
                     ADTLYVGDSIIDAQTGMNAGIDFAAVLNGMTRREEFYEYPSVKIMDSFSELL"
BASE COUNT          294 a          164 c          249 g          316 t
ORIGIN      
        1 cctgccattt ttttgcatct tgtatacaat gccgtgccgt ttgtgataca ggtattgccg
       61 gaagcagttt tattaaacac tgttttttgg ctgctgacac catttttgct gctgcttatc
      121 ggtctttttc tgttcaggat gttcggattt tcttttagct taaggaaggt atccgctgac
      181 gtatcggacg cagttcgtca ataattgaaa ataatttcat accatttgtt tataagtgta
      241 ttgatcggct tatggacgaa tggtattttt ttatttataa ggcttcatgg aaattcctgt
      301 gtttcattta ttgcttcaaa ataaaaaatg tgatagcata ataaaaagat aagaagagga
      361 gttatatgat catttatgaa agagtatcga ttatatttgt ttgattttga ctatacgctc
      421 gtaaattcgg aaaaaggaat tgtcggctgc tttgagcatg tattctggct gaatgggatt
      481 accggaatag aaaaagatga tatcaggaga acgatcggaa tgccgcttgc agaagaattt
      541 cgtgttctta caggagttga agataaacac atgctggaac gcttcaggcg ggaattttct
      601 gaaatggccg atgaaatcat gaccgacagg acggagcttt ttccggaaac gcttcctatg
      661 ctggaaagac tgaaaaaaag gaatgcacga attgggatcg tatcaacaaa acagcggccg
      721 cgtattcggg aagccattga taaattttac ttacaggaat tgatcgatct ggttatcggt
      781 gtggcagatg tgacacagac gaagccgaat ccgcagggag ttcttcgtgc gatcgacttt
      841 tttaaaattg acaaggctga tacgctgtat gtcggagaca gtattatcga cgcgcagacg
      901 ggaatgaatg ccggtattga ttttgcagcc gtattgaatg ggatgacaag gcgtgaagaa
      961 ttttatgaat atccgtctgt gaaaatcatg gattcattca gcgagctgct gtgaggaagg
     1021 aac
//
LOCUS       DAOSYE010000925         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_145912_length_1018_cov_2.794393, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000925 DAOSYE010000000
VERSION     DAOSYE010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145912_length_1018_cov_2.794393"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLU43_12710"
     CDS             <1..608
                     /locus_tag="PLU43_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29319.1"
                     /translation="IFTFRRPVQRSGKSTLMNVIGALDRPTEGEYTLDGVNIFHAKDK
                     DLSGIRCKKIGFVFQTYNLVSRTSALKNVELPMLYSKMPRSARTKRAKELLGMVDMEE
                     RMKHSPDELSGGQKQRVAIARAMANDPAIILADEPTGALDSSTGRLIMDIFHKLNREQ
                     GKTIVLITHSNELAEETSRILTLKDGVIIGCRKGSGYDAFH"
     gene            592..>1018
                     /locus_tag="PLU43_12715"
     CDS             592..>1018
                     /locus_tag="PLU43_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29320.1"
                     /translation="MMLFIENVLLAFGSLLANKMRALLTMLGIIIGIGSVIAIVTVGN
                     SLTNSITASMESMGANNITVGLQQKSTAEETTDSGMTFRGPTWQKQATEDDYFTDEML
                     DELLEKFPDEIKDLSISETLGTGEVTIGTDYANVSVMGVN"
BASE COUNT          291 a          200 c          276 g          251 t
ORIGIN      
        1 gtatattcac cttccgtcgg cctgtccagc gctccggtaa atctacattg atgaatgtga
       61 tcggagcgct ggacaggccg acggaaggtg aatatacact ggatggagtg aatatatttc
      121 atgcaaagga taaagatctg tccggtattc gatgtaaaaa aattggattt gtatttcaga
      181 cttataatct tgtgtcaagg acgagcgcac taaaaaatgt ggaacttccg atgctgtatt
      241 ccaagatgcc gagaagcgcc agaacaaaga gggcaaaaga acttcttggt atggtagata
      301 tggaagaacg tatgaagcat tcaccggatg agctttccgg tggtcagaaa caaagggtgg
      361 cgatcgcaag agctatggcg aacgatccgg cgatcattct ggccgatgaa ccgaccggtg
      421 cactggattc cagtaccgga cgtctgatca tggatatatt tcacaaattg aatagagaac
      481 agggcaagac gatcgtactg attacgcatt cgaacgaatt ggccgaagaa acgagccgga
      541 tcctgacatt gaaggatggc gtgatcattg gctgcaggaa ggggagtgga tatgatgctt
      601 ttcattgaaa acgtacttct tgcatttggc tccctgcttg cgaataagat gcgcgccctg
      661 cttacgatgc ttggtattat cattggtatt ggttcagtta tcgcgatcgt aacagtcgga
      721 aattcgctga ctaattccat taccgcatct atggagtcaa tgggtgccaa taatattaca
      781 gtcggtctgc agcaaaaatc gactgcggaa gaaacaacgg acagcggcat gacgtttcgg
      841 ggccctacgt ggcagaaaca ggcgacagag gatgattatt ttacagacga gatgcttgat
      901 gagctgctgg agaagtttcc ggatgaaatt aaggacctat ctatcagtga gacacttgga
      961 accggtgagg tcacgatcgg caccgattat gcaaatgtat ctgttatggg tgtcaacg
//
LOCUS       DAOSYE010000926         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_145927_length_1018_cov_2.635514, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000926 DAOSYE010000000
VERSION     DAOSYE010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145927_length_1018_cov_2.635514"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="dprA"
                     /locus_tag="PLU43_12720"
     CDS             <1..904
                     /gene="dprA"
                     /locus_tag="PLU43_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO29321.1"
                     /translation="FQLSQVPNGNRKQDICFIPWYHESYPKKLRQISGYPFAVFVKGR
                     LPDEKAGSVAIIGARNCSEYGRSMARYFGEELARSGVQVISGMAYGVDGIGQMCALEA
                     GGYSCGILGCGVDVCYPASNQTLYARLAKQGGLLSEYCPGTPPVARQFPPRNRIISGL
                     SDIVLVVEARKKSGTLITVDMALEQGKEILVVPGRVTDPLSSGCNSLLKQGASPVTEP
                     SDVLALLEGKYEIKVKKTKKEKIMLESNEKLVYCCCGLYAKNLEDIASEVSCSYTELL
                     HTLVSLELKGYIREISKNYYVRTS"
BASE COUNT          304 a          182 c          283 g          249 t
ORIGIN      
        1 gtttcagtta agtcaggttc cgaatggaaa cagaaaacag gatatatgtt ttatcccatg
       61 gtatcatgaa agctatccga aaaagctgcg gcagatatcc ggttatccgt tcgccgtatt
      121 tgtaaaaggc agacttcctg acgaaaaagc agggagcgtg gcgatcatcg gcgcgcggaa
      181 ctgttccgaa tatggaagaa gcatggccag atattttgga gaggaactgg ctcgcagcgg
      241 agtgcaggtg atcagcggca tggcgtatgg agtcgatggg atcggacaga tgtgtgcgct
      301 tgaggcgggc ggctattcct gcggtatatt agggtgcggt gtggatgtct gttatccggc
      361 ttccaatcag actctttatg cgcgcctcgc aaaacagggc ggactgctgt cagagtattg
      421 tccaggaacg ccgcctgttg caaggcagtt cccgccgagg aacagaatca tcagcgggct
      481 ttccgatatt gtactggtag tggaggcaag gaaaaagagc ggaacactga tcacggtcga
      541 tatggcactt gaacagggaa aggaaattct tgttgtgccg ggaagagtga cggacccatt
      601 gagcagcggc tgcaacagcc tgttaaaaca gggcgcaagt cctgttacag agccatctga
      661 tgtacttgca cttttggaag gaaaatatga aataaaagtt aaaaaaacaa aaaaagaaaa
      721 aataatgctt gaaagcaacg aaaaattagt gtattgttgt tgtggtttgt atgcgaaaaa
      781 tttggaagat attgcatcgg aagtatcctg ttcctatacg gagctgctgc atacattagt
      841 atccctggaa ttaaaaggat atatcaggga aatcagtaaa aattactatg tccgtacttc
      901 gtaacagcaa actgtttcgg gaatctggag tatggtacgg tgtgggagga atttaaatgg
      961 caaaatatct ggtaatcgta gagtcgcctg caaaggtgaa aactattaaa aaattttt
//
LOCUS       DAOSYE010000927         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_147358_length_1012_cov_4.521421, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000927 DAOSYE010000000
VERSION     DAOSYE010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147358_length_1012_cov_4.521421"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLU43_12725"
     CDS             <1..303
                     /locus_tag="PLU43_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29322.1"
                     /translation="LSGGEQQMLAMGRALMSRPKILLMDEPSMGLSPIYVNEIFDIIR
                     EVSEGGTTVLLVEQNAKKALSIADRAYVLETGKIVLSGRAEDLLNNDSIKKAYLGE"
     gene            322..939
                     /locus_tag="PLU43_12730"
     CDS             322..939
                     /locus_tag="PLU43_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOO29323.1"
                     /translation="MEHVIVTIARQYGSGGKTIGQMLADDMNVSCYEREILHMASEDS
                     GINEYLFAQVDERIRTNTFFKMKHIYKGALVTPESDGFTSDDNLFNYQAKIIKKLAEK
                     DSCVIIGRCADYILKDYPNVVSIFVHADPQFCKKSALERVSVPEKDIMKYIEKTDKYR
                     GDYYKAHTGHEWHDLRNYDLCLNSASLGFEKCVEEIKAYIKIRFN"
BASE COUNT          333 a          194 c          232 g          253 t
ORIGIN      
        1 ctcagcggcg gagaacagca aatgcttgcc atgggcaggg cactgatgtc aaggcccaaa
       61 atccttctga tggatgaacc gtccatggga ctttctccaa tttatgtgaa tgaaattttt
      121 gatattatca gggaggtcag cgaaggcggc acaacagttc tgcttgtaga gcagaacgcg
      181 aaaaaagcgc tttccattgc agaccgcgct tatgtactgg aaacaggtaa aatcgtactt
      241 tccggccggg ctgaagacct gctgaacaac gattcgatca aaaaagccta tttaggcgaa
      301 taagaaagga aggcttcggt tatggaacat gtgatcgtta caatcgcccg ccagtatggc
      361 agcggcggga aaacaattgg acaaatgctt gcggacgaca tgaatgtcag ctgttatgaa
      421 agggaaatac tgcatatggc ttccgaagac agcggcatca atgaatacct gtttgcacag
      481 gtagacgagc gtattcgcac aaacacattt ttcaaaatga aacatattta taaaggggcc
      541 ctagtcacac cggaaagcga cggctttact tccgatgaca atctgtttaa ctatcaggct
      601 aaaattatca aaaaactggc tgaaaaagat tcctgcgtga ttatcgggcg ctgcgcggac
      661 tatattctga aggattatcc gaacgtcgta tccatatttg tccatgcaga tcctcagttc
      721 tgtaaaaaaa gcgcactgga acgcgtttct gttccggaaa aagatattat gaaatatatt
      781 gaaaaaacag ataaatacag aggtgattac tacaaagcac ataccggcca tgaatggcat
      841 gatctgagaa attatgactt atgtctgaac agcgcaagtc tcggatttga gaagtgtgtg
      901 gaagaaataa aggcatatat taaaatacgt tttaattaat gaagaaaaat ccgtattgtt
      961 ttcgatacaa cgcttgaatt tccatcctaa aaattatata ttagaaaagg tg
//
LOCUS       DAOSYE010000928         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_147395_length_1012_cov_3.076280, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000928 DAOSYE010000000
VERSION     DAOSYE010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147395_length_1012_cov_3.076280"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1012)
                     /locus_tag="PLU43_12735"
     CDS             complement(<1..>1012)
                     /locus_tag="PLU43_12735"
                     /inference="COORDINATES: protein motif:HMM:NF039205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOO29324.1"
                     /translation="LVGKLLSFARSGAKIELEGEAYDCVDLLILFVRRSLNLLSVLTS
                     PDQVEAMIFTVDTLEGRTIEVLDKIADAVPVARNKIFFQTYEESSYYYVLHQPRELWQ
                     NEVVIFDYSNQYMRAYELWMNRKTVPVVAFVDRMDFEEVKMPQLMMEGEPSREKEERL
                     DELILRTMHEFFSGKTIGTVFLIGSGFEESWCDKTLKYLCMGKRVFQGKNLYSKGACY
                     CAKDKITPCELNKNYVFLGKDKLKFNLGIRMYKQGQEEYIALADAGENWYDTTAQVDF
                     ILGNTYRVPLVITPLDGKEQQEIEIELTGLPERPPKATRLQMKVNFKSETGIFARIYD
                     LGF"
BASE COUNT          257 a          286 c          183 g          286 t
ORIGIN      
        1 cgaaaccaag gtcgtagatt ctggcaaata tgcctgtctc cgatttaaaa ttcactttca
       61 tctgcagtct tgtcgccttc ggcggccttt ccggaagacc ggtcagctct atttcgatct
      121 cctgctgttc cttcccgtca agcggcgtga tcacaagcgg aaccctgtat gtattcccaa
      181 gaataaaatc cacctgagcc gtcgtatcat accagttttc tcctgcatcg gccagtgcaa
      241 tatattcttc ctgtccctgt ttgtacatcc ggatgccaag attgaacttc agcttatctt
      301 ttccgagaaa gacataattt ttattcagtt cacacggcgt tattttatct ttcgcacagt
      361 aacaggcccc cttggaatac aggtttttcc cctgaaacac tctttttccc atgcacagat
      421 attttaaggt cttgtcacac cacgattcct caaatccgct gccgatcaga aataccgtcc
      481 cgatcgtctt tcccgaaaaa aattcatgca ttgtacgcag gatcaattca tcaagccgtt
      541 cttccttctc tcttgacggt tccccttcca tcatcagctg cggcattttg acttcctcaa
      601 aatccattct gtccacaaac gcaacaacag gaactgtctt tctgttcatc cagagctcat
      661 aggcacgcat atactggttg gaataatcaa aaataacgac ctcattctgc caaagctccc
      721 ttggctgatg aagtacataa taataggagc tttcctcata tgtctgaaaa aagattttat
      781 tccgcgcgac cggaacagca tcggcaattt tatcaagcac ctcgatcgta cgcccttcca
      841 gcgtatctac cgtaaatatc atcgcttcca cctgatccgg ggatgtcagt acggaaagca
      901 ggtttaagga ccttctcaca aaaaggatca gcaggtccac gcagtcataa gcctcgccct
      961 ccagctcgat cttggcgccg gatctggcaa agcttaaaag ctttcctacc ag
//
LOCUS       DAOSYE010000929         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_147688_length_1011_cov_2.996862, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000929 DAOSYE010000000
VERSION     DAOSYE010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147688_length_1011_cov_2.996862"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLU43_12740"
     CDS             <1..402
                     /locus_tag="PLU43_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOO29325.1"
                     /translation="DIPKELRSTLGKTTTQRLDRFIHDIIINSINKNDICMSDEIGEA
                     MMELRTFMFINVYQNPKAKGEEGKAERLVEALYQYFLEHLEELPAEYLHIMEVHKECM
                     DRVVCDYVSSMSDRFAIGIFSDIYLPDSWKD"
     gene            429..>1011
                     /locus_tag="PLU43_12745"
     CDS             429..>1011
                     /locus_tag="PLU43_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOO29326.1"
                     /translation="MYYPDELVEEIRLKSDIVEVISSYVRLQKKGSNYFGLCPFHNEK
                     SPSFSVSPAKQIYYCFGCGAGGNVITFIMEYENYTFVEALKFLAERAGVELPEISYSE
                     EARKKDDLKSRLLEVSKEAGRYYYYQLRAESGKQAYTYLSGRGLSDDTIKKFGLGFAK
                     TGRNTVYQYLRGKGYKDELLRQSGIFVFDERHGM"
BASE COUNT          298 a          177 c          269 g          267 t
ORIGIN      
        1 gacatcccta aagaactgcg cagtacgctt ggaaaaacaa cgacgcagcg cctggaccgg
       61 tttattcatg acattattat aaacagtatc aataaaaacg atatctgtat gtcggatgag
      121 atcggcgagg ccatgatgga actgcgtacc tttatgttta ttaacgtcta tcagaatccg
      181 aaggcaaaag gcgaggaagg gaaagcggaa cgccttgtgg aagcacttta tcagtatttt
      241 ctggagcatt tagaggagct tccggctgag tatctgcata ttatggaagt acacaaggag
      301 tgcatggaca gggtggtgtg cgattatgta tcgtcaatgt cggatcgctt tgcgatcggg
      361 atattcagcg atatttatct tccggattcg tggaaggact gattcacagc agacaaagag
      421 gggtttcaat gtattatcct gatgaacttg ttgaggaaat ccgtcttaag agtgatattg
      481 ttgaggtgat atcctcttat gtacggcttc aaaaaaaggg gagcaattat tttggactgt
      541 gtccctttca taatgaaaaa tcgccttctt tttccgtaag tccggcgaaa cagatctatt
      601 attgcttcgg atgcggtgca ggcggaaatg tgatcacttt tattatggag tatgaaaatt
      661 acacatttgt cgaggcgctg aaatttcttg ccgaaagggc gggtgtagag cttccggaaa
      721 tcagttattc ggaagaagcg aggaaaaaag acgacttaaa gtccagactg cttgaagtca
      781 gcaaggaagc aggcaggtat tattactatc agcttcgtgc ggaaagcgga aagcaggcat
      841 atacctatct aagcggcaga ggactttcag atgatacgat aaaaaagttc ggacttggtt
      901 ttgcaaagac cggcaggaat acggtatacc agtatctgcg cggaaagggt tacaaggatg
      961 aactgctgcg tcagagcggt atttttgtgt ttgacgaaag acacggaatg a
//
LOCUS       DAOSYE010000930         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_148204_length_1009_cov_2.796646, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000930 DAOSYE010000000
VERSION     DAOSYE010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148204_length_1009_cov_2.796646"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLU43_12750"
     CDS             complement(<1..353)
                     /locus_tag="PLU43_12750"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO29327.1"
                     /translation="MSRKKIIAGNWKMNMTPSEAVKLCGELKDLVKSDTVDVVYCVPA
                     IDLVPVVEAVKGTNVEVGAENMYFEEKGAFTGEISAAMLKDAGVKYVIIGHSERREYF
                     KEDDALLNKKVKKAF"
     gene            complement(398..>1009)
                     /gene="pgk"
                     /locus_tag="PLU43_12755"
     CDS             complement(398..>1009)
                     /gene="pgk"
                     /locus_tag="PLU43_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO29328.1"
                     /translation="GAKVADKLNVINNLIEKCDTLIIGGGMAFTFLKAKGYEIGKSLV
                     DNEKLDYCKEMMEKADKLGKKLLLPVDTTVAAEFPNPIDGPIEVSVVDADKIPEDMEG
                     LDIGVKTSALYAEAVKTAKTVVWNGPMGVFENPVLAKGTIAVAEALAETDATTIIGGG
                     DSAAAVNQLGFADKMSHISTGGGASLEFLEGKELPGVAAADDK"
BASE COUNT          241 a          279 c          165 g          324 t
ORIGIN      
        1 tcaaacgctt ttttcacctt tttattcaaa agtgcatcgt cttccttgaa atattcccgt
       61 ctctcggaat ggccgatgat cacatatttc acgcctgcat ctttcagcat tgctgcggaa
      121 atctcgcctg tgaaagcccc tttttcttcg aaatacatat tttcagcgcc tacttccaca
      181 tttgttccct ttaccgcttc aactaccgga acaagatcaa tcgcaggtac acaatataca
      241 acatcaacag tatctgactt tacaagatcc tttaattccc cgcacagctt gaccgcttca
      301 ctcggagtca tattcatttt ccagttaccg gctatgattt tctttcttga cattttcatt
      361 tcctctttct ttctgttaat aattataatt ttcgctccta tttatcgtct gctgcagcaa
      421 ctccaggcag ttccttgcct tccaggaact cgagcgaagc tccgccgcct gtggaaatat
      481 gagacatctt atccgcaaat ccaagctgat ttacagctgc agccgaatca ccgcctccga
      541 tgatcgttgt agcatctgtt tcagcaagcg cttccgctac ggcaatcgtt cctttggcaa
      601 gtacaggatt ttcaaataca cccattggtc cgttccatac aactgttttt gctgttttca
      661 cggcttctgc ataaagtgcg gatgttttca caccaatatc aaggccttcc atatcctccg
      721 gaatcttgtc agcgtcaacg actgacactt cgatcggtcc gtcaatcgga ttcggaaact
      781 ctgccgcaac ggtcgtatca acaggaagaa gcagcttttt gccaagctta tcggcctttt
      841 ccatcatttc cttgcagtaa tccagttttt cattatccac aagcgatttt ccgatctcat
      901 agccttttgc ctttaagaaa gtaaatgcca ttccgccgcc gataataagc gtatcacatt
      961 tctcaatcag attattgatc acgttaagct tatctgccac ttttgcacc
//
LOCUS       DAOSYE010000931         1008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_148550_length_1008_cov_1.883526, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000931 DAOSYE010000000
VERSION     DAOSYE010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148550_length_1008_cov_1.883526"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1008
                     /locus_tag="PLU43_12760"
     CDS             34..>1008
                     /locus_tag="PLU43_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOO29329.1"
                     /translation="MPGVSVHDNEWQFYCVSDCRHSLVLCLYENTGQLYAQIDMLPFK
                     IAGNVYSVRITGLEEKDLEYAYEADGRPCCDPYKKKSAGHSKWGEFDIDGENIRSGFY
                     RDEFDWGDDVPLKLPYNQIIAYSMHVRGFPKHSSSGVKAKGTYLGIIEKIQYLKELGI
                     NQIELLPIYEFYELDVEKELLPEGHPKYLKDRKEPPEAKINYWGFKYGSYYVPKASYA
                     YGEDSTAEFKTLVKELHKNGIEIILQFYFPNEINRTVILDCLKFWTINYHVDGFHIMG
                     EHLPIDFIASDPLLTDTKLYYAYYDKEHIFLGKYEHNHFLAEVNQEYMN"
BASE COUNT          316 a          184 c          238 g          270 t
ORIGIN      
        1 cacaacttac atttaaaaaa gggagacagt cttgtgccgg gcgtcagtgt tcacgataat
       61 gaatggcaat tttattgtgt ttcggactgc cggcattctc ttgtattatg tctctacgaa
      121 aataccgggc agttatatgc gcagatcgac atgctgccgt ttaaaattgc cggtaatgtc
      181 tattcggtca gaatcacggg acttgaggaa aaggatctgg agtatgctta tgaggcggac
      241 ggaagaccct gctgtgatcc gtataaaaag aaaagcgccg gacacagtaa atggggtgaa
      301 tttgacattg acggggaaaa tataaggagc ggtttctata gagatgagtt tgactggggg
      361 gacgatgtcc ctctgaagct tccttataat cagatcattg cctattccat gcatgtacga
      421 ggctttccaa agcattcgtc ttcaggagtc aaagcaaaag gaacgtatct tgggatcatc
      481 gaaaaaatac aatatttaaa agaactcgga atcaatcaga tcgaactgct tccgatttat
      541 gaattttatg aactggatgt ggaaaaggaa ctgctgccgg aaggtcaccc gaaatattta
      601 aaagaccgga aggagccgcc ggaagccaaa atcaattact ggggattcaa gtacggcagc
      661 tactatgtgc cgaaggcatc ttatgcctat ggagaagaca gtacggcaga atttaaaaca
      721 cttgtaaagg agcttcataa gaacggcatt gagatcatac tgcaattcta ttttccgaat
      781 gagatcaacc ggacggtgat cttagattgt ctgaagttct ggactatcaa ttatcatgtg
      841 gatggttttc atataatggg ggaacacctg ccaatcgatt ttatcgcttc agatccactt
      901 ttaacagata caaagctgta ttacgcctat tatgataaag aacatatttt tttgggaaaa
      961 tatgagcaca atcatttcct ggcggaagta aaccaggaat acatgaat
//
LOCUS       DAOSYE010000932         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_149487_length_1004_cov_2.473130, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000932 DAOSYE010000000
VERSION     DAOSYE010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149487_length_1004_cov_2.473130"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>1004)
                     /locus_tag="PLU43_12765"
     CDS             complement(64..>1004)
                     /locus_tag="PLU43_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOO29330.1"
                     /translation="LYQVGSFPSWNRFAEWNGKYRDDMRRFLKGDNGMAQAAALRLTG
                     SADLYPEKTRGRNASVNFLNCHDGFTLYDLYSYNEKHNEANGWDNTDGDNNGHSWNCG
                     TEGETDDAAIQGLRRKMIKNACVSLLCSRGTPMFYAGDEFGNTQYGNNNPYCQDNEIS
                     WLDWSLLKKNEDIYEFFKFMIHFRRRHAVLRKNLSVCSLGFPFVSTHGNQPFVAEFSY
                     ESKQVGVMFAGRSRGRDDIVYAAFNAYWGMETMILPDLKGSGCWQVVVDTAQEKPLKK
                     RILPDGRLTIGARSAVVLVYVPSGSYICQTYKNENS"
BASE COUNT          243 a          277 c          198 g          286 t
ORIGIN      
        1 gacttctcgt cgccggtccg atcgtacaga caatttttgt ttttctcatt ttatgattct
       61 cctctaactg ttttcgtttt tataagtctg acagatatag ctgccgcttg gtacatagac
      121 aagcacaacc gcgctccgtg ctccgatcgt cagtcggccg tcaggaagta tccgtttttt
      181 caaaggcttt tcctgagctg tgtctacaac tacctgccag catccgctgc ctttcagatc
      241 aggcaggatc atggtttcca ttccccaata ggcattgaat gccgcataaa caatgtcgtc
      301 tctcccgcgg ctgcgtcctg caaacatcac gcccacctgt ttgctttcat aagaaaactc
      361 cgctacaaac ggctggtttc catgcgtact gacaaaagga aatccaagcg aacagacaga
      421 aagatttttt cgaaggaccg catgacgtct tctgaaatgg atcataaatt taaaaaactc
      481 atagatatct tcgttcttct tcaaaaggct ccagtcgagc catgagatct cattgtcctg
      541 acagtatgga ttattattgc cgtactgcgt gtttccaaac tcgtcccccg catagaacat
      601 cggcgtacct ctgctgcaga gcagactgac acaggcattt tttatcattt ttctgcgaag
      661 cccctgaatc gccgcatcat ccgtttcccc ttcagtgccg cagttccagg aatgtccgtt
      721 attgtccccg tccgtattgt cccagccatt tgcttcatta tgcttttcat tgtaggaata
      781 cagatcgtat aaagtaaacc cgtcatgaca attcaaaaaa ttgaccgaag catttcttcc
      841 gcgcgttttc tccggataca gatcggcaga gccggtcagc ctgagtgcgg ccgcctgtgc
      901 cattccgtta tcgcccttta aaaaccggcg catatcgtct ctgtattttc cattccactc
      961 agcaaaccgg ttccacgaag gaaaactgcc gacctgataa agac
//
LOCUS       DAOSYE010000933         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_149488_length_1004_cov_2.472076, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000933 DAOSYE010000000
VERSION     DAOSYE010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149488_length_1004_cov_2.472076"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="flgK"
                     /locus_tag="PLU43_12770"
     CDS             complement(<1..684)
                     /gene="flgK"
                     /locus_tag="PLU43_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOO29331.1"
                     /translation="MSSTFFGLNIAYTGLQAASAALNTTGNNISNVETDGYTRQEVIQ
                     QAAAALRTNTTYGMAGAGVETTEITQIRNEYYDLKYWSNNADLGEYDIKQYFMEQIED
                     YFTETDTVEGFGTIFSDMFSGLTEVYNSSGDDTKVSAFLSLAGNLCEYFNAMSTDLTQ
                     LQEEANEEIKNKTEEINSIASQIATLNQQINVIEITGVTANELRDKRALLIDELSAIV
                     DVEVTETPIY"
     gene            complement(728..>1004)
                     /locus_tag="PLU43_12775"
     CDS             complement(728..>1004)
                     /locus_tag="PLU43_12775"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOO29332.1"
                     /translation="LNPNVTSTYDFADYYSAIVSQVANTGSVYNSISTSQEATVDALD
                     EARQSVMGVSSSEELTNMIKYQNAYNAASRYINAVNEMLGHIIEKLG"
BASE COUNT          239 a          253 c          183 g          329 t
ORIGIN      
        1 atagatcggt gtttcagtca cctccacatc cacaatggca gaaagctcat cgatcagaag
       61 cgctctcttg tctctgagct catttgccgt tacacctgtt atttcaatga cattgatctg
      121 ctggttcagc gttgcgatct gcgaagcgat cgaattgatc tcttccgttt tgttcttgat
      181 ctcttcattt gcctcttcct gcaattgcgt cagatccgtt gacattgcat taaaatattc
      241 acagagattt cctgccagcg ataaaaatgc agatactttc gtatcatctc ctgagctgtt
      301 atagacttcc gtaagaccgg aaaacatatc ggaaaagatc gtgccaaatc cttctaccgt
      361 atctgtttcc gtgaaataat cctctatctg ctccataaaa tactgcttga tatcatactc
      421 tccaaggtcc gcattattac tccagtactt cagatcataa tattcattac ggatctgtgt
      481 gatctctgtc gtttcaacac cagctcctgc cataccgtac gtcgtatttg tgcggagtgc
      541 ggctgctgcc tgctgaataa cttcctgtct cgtatatccg tctgtctcta cgtttgaaat
      601 attgtttcct gtcgtattca gggctgcgct tgctgcctgc agacccgtat atgcaatgtt
      661 caatccaaaa aatgttgacg acatcagatc acctctttcc gtttatttcc tctgttctgt
      721 tcatctatta accaagtttt tcgataatat gtccgagcat ctcattgact gcattgatat
      781 aacggcttgc cgcattatat gcattctgat atttgatcat attcgtaagc tcctcgcttg
      841 aagaaacgcc catgaccgac tgtcttgctt cgtcaagcgc atccacagtt gcttcctgac
      901 tcgtcgatat actgttgtat acggagccgg tattggcaac ctgactcacg atcgccgaat
      961 aataatctgc aaaatcataa gtgctcgtca catttggatt cagt
//
LOCUS       DAOSYE010000934         1002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate AMR_MDS_2093
            NODE_149926_length_1002_cov_3.559662, whole genome shotgun
            sequence.
ACCESSION   DAOSYE010000934 DAOSYE010000000
VERSION     DAOSYE010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864160
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (wastewater metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,539
            Genes (coding)                    :: 2,518
            CDSs (with protein)               :: 2,518
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 12 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149926_length_1002_cov_3.559662"
                     /isolate="AMR_MDS_2093"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..796
                     /locus_tag="PLU43_12780"
     CDS             92..796
                     /locus_tag="PLU43_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO29333.1"
                     /translation="MQLVICDDNKAVIKELEKKLKTRYADALSIHSFSTVFSALTYVH
                     DIAKGDVDMAILDINIEDGNGIELARKLQKLYPHIKVIFLTGYLHYAADIFQATPVYF
                     LVKPIQDERLFEAMDRAIVSVDKLRHDMLQVSIKGKICRVLMEDIRYIESEKRTLHIY
                     EKNGKISVLKQINAIEPELPEQFLRCHQSYCVNMNWIKEFASDIFLYSGERIPVSRKR
                     YKEAKQKFLRFIGDQI"
BASE COUNT          321 a          169 c          226 g          286 t
ORIGIN      
        1 cagtttgaag tcactcaata aacgatgttc tcgtcacgag tcacgagaat ctagggttta
       61 taataaataa aatatatggg aggagataga agtgcagctg gtaatttgtg atgacaacaa
      121 ggctgtcatt aaggaattgg aaaaaaaatt aaaaaccaga tacgcggatg ctctgagtat
      181 tcattcgttt tctacagtct tttcagcgct tacttatgta cacgatattg caaagggaga
      241 tgtggacatg gcgattctgg atatcaacat agaggatgga aacgggatcg aactggccag
      301 gaagctgcaa aagctgtatc cgcacatcaa ggtaattttt cttaccggat atctgcatta
      361 tgcggcagat atttttcagg caacaccggt ttatttttta gtaaaaccaa ttcaggatga
      421 aaggcttttt gaagcaatgg accgtgcaat cgtgtcggtt gataaattaa gacacgatat
      481 gctgcaggtc agtatcaaag ggaagatctg cagggttctt atggaagaca tccgttatat
      541 tgaaagtgag aaaagaacgc ttcatattta tgagaaaaat gggaaaattt ctgtcctgaa
      601 acagataaat gccatcgagc cggaactgcc ggaacagttt ttaagatgtc atcagagtta
      661 ttgtgttaat atgaactgga tcaaagaatt tgcttccgat atttttcttt attcaggaga
      721 aagaattccg gtcagcagaa agcggtataa agaagcaaaa caaaaatttc tccggtttat
      781 aggtgatcag atctgaaagg ctgtttatca aaatacgcca tcccctctgc ctgaagggac
      841 ggcgtatttt caatcatatg acagactcat taatgtgtat tattataaag tgtctgcaat
      901 tctgcacatg tgtggtggct gatcatttct tctgcctgcc cggtataatc ccaataggaa
      961 aggaaataat tccactgctc agtcgtaaag acagtcgttg tg
//
